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<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU18</accession>
    <name>A0A1E3NU18_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein L17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_81778</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL17 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454220">
        <property type="protein sequence ID" value="ODQ56669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035876.1">
        <property type="nucleotide sequence ID" value="XM_019185293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU18"/>
    <dbReference type="STRING" id="683960.A0A1E3NU18"/>
    <dbReference type="GeneID" id="30202539"/>
    <dbReference type="OrthoDB" id="275000at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.10">
        <property type="entry name" value="Ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000456">
        <property type="entry name" value="Ribosomal_bL17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036373">
        <property type="entry name" value="Ribosomal_bL17_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00059">
        <property type="entry name" value="L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL17M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413">
        <property type="entry name" value="RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01196">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64263">
        <property type="entry name" value="Prokaryotic ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ56669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="24583" checksum="97619DEA19867641" modified="2017-01-18" version="1">MPVRHFSRTGSHRRAMMRNLVTSLIEHESIITTHAKAKETQKLADKLISLTKKDNITQAKVDAGEDLFKLKTTIPKLFQDLRTRFAKREGGFTRVLRLEPRLGDRAEQSIVELVDGPKEMKLWMTARVVARLQLQNLPVDDLTQHNVNKLIQFRAGGQEEFDALVERCKKEFYRDGKSEDLKNLPPPAENMRKPYTGYKLVKGFKVMPRPTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU71</accession>
    <name>A0A1E3NU71_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_65912</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454250">
        <property type="protein sequence ID" value="ODQ56624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035831.1">
        <property type="nucleotide sequence ID" value="XM_019184023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU71"/>
    <dbReference type="GeneID" id="30201269"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ56624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="53" mass="5665" checksum="A11AA82C3F7D7ABF" modified="2017-01-18" version="1">METTASQRSSHVQGGITMENRGGNTTSGVEIGDPVLLFGKLWQELGCGERNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUD0</accession>
    <name>A0A1E3NUD0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Phosphatidylinositol transfer protein SFH5</fullName>
            <shortName evidence="16">PITP SFH5</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_86203</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Non-classical phosphatidylinositol (PtdIns) transfer protein (PITP), which exhibits PtdIns-binding/transfer activity in the absence of detectable PtdCho-binding/transfer activity. Regulates PtdIns(4,5)P2 homeostasis at the plasma membrane. Heme-binding protein that may play a role in organic oxidant-induced stress responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)</text>
            <dbReference type="Rhea" id="RHEA:38691"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:38692"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 16">Endoplasmic reticulum membrane</location>
            <topology evidence="2 16">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Microsome membrane</location>
            <topology evidence="16">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the SFH5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035945.1">
        <property type="nucleotide sequence ID" value="XM_019186011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUD0"/>
    <dbReference type="STRING" id="683960.A0A1E3NUD0"/>
    <dbReference type="GeneID" id="30203257"/>
    <dbReference type="OrthoDB" id="75724at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000114">
        <property type="term" value="P:regulation of establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017157">
        <property type="term" value="P:regulation of exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042938">
        <property type="entry name" value="Sfh5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47669">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN SFH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47669:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN SFH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12 16" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 16" id="KW-0492">Microsome</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="17">
        <location>
            <begin position="142"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ56738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="293" mass="34186" checksum="9D5695A960EB091C" modified="2017-01-18" version="1">MSNVSVKTAKFESDHQREKFDEFVDLHLPDILNQAQYDELFGYQLSSQGEYFNKDVFDALIFKYLKANDFQIQESKDQLLNTLIWRKNFNPLSAAFNETHDSKFDDIGVLTNYGNNTANTKIITWNLYGAGGNPKELFKDLEKFLRYRIGLMERSVQLLDFTNEENCFMTQVHDYKGVSFLRIDPDIKKGSKATIQIFQDYYPELLYKKFFVNIPSVLFWVFEFMKKLLPTETTKKFIILNHSTGIYKYLGDEVPSKNYGGKGKDLELQNVKEIKPPVYGAHLSQQLFTNEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUK4</accession>
    <name>A0A1E3NUK4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_23142</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036032.1">
        <property type="nucleotide sequence ID" value="XM_019180976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUK4"/>
    <dbReference type="STRING" id="683960.A0A1E3NUK4"/>
    <dbReference type="GeneID" id="30198222"/>
    <dbReference type="OrthoDB" id="2155283at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050897">
        <property type="entry name" value="SMAUG/VTS1_RNA-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12515:SF5">
        <property type="entry name" value="PROTEIN SMAUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12515">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="SAM" evidence="4">
        <location>
            <begin position="3"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ56825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="58" mass="6759" checksum="A66832E6D72D93B2" modified="2017-01-18" version="1" fragment="single">KLLNDIPAWLKTLRLHKYTSALQDVPWRELIYYDDQQLELKGVSAMGARGKLLKAFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV59</accession>
    <name>A0A1E3NV59_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_70942</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036181.1">
        <property type="nucleotide sequence ID" value="XM_019184225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV59"/>
    <dbReference type="STRING" id="683960.A0A1E3NV59"/>
    <dbReference type="GeneID" id="30201471"/>
    <dbReference type="OrthoDB" id="272512at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ56974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="279" mass="31916" checksum="055ECCCC4C4C5435" modified="2017-01-18" version="1">MERYTQFRDKATGISPFLPVLQTNVIGVNKRDDLLKTLKLDVALSFLIFIVKLVFVIPILILCLITLGQVHYLNRCLLFVMGLYSYELSIQNLKSRTNISKNFYPSKNEVFLVNFTSPLDLCVLKLISNDDFVILIPNSKGDLVEISYLEFIQQSLKGDVLDKIEKIDYNKITNKIIYIFPEGTTSNGKAILPFLLTQDSLNNFISNFNPLKIKTLGIKLYPSTLTTPLNYSIQSYLYKLLTLLSFEIKIRINSPNELDSKDSNLLKLRNSFTNNAKIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVA7</accession>
    <name>A0A1E3NVA7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ras-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_15045</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036242.1">
        <property type="nucleotide sequence ID" value="XM_019180626.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3NVA7"/>
    <dbReference type="GeneID" id="30197872"/>
    <dbReference type="OrthoDB" id="775356at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110085">
        <property type="term" value="C:mitotic actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903479">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21206">
        <property type="entry name" value="CH_IQGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12206">
        <property type="entry name" value="RasGAP_IQGAP_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000593">
        <property type="entry name" value="RasGAP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149:SF14">
        <property type="entry name" value="CALPONIN-HOMOLOGY (CH) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN WITH IQ MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03836">
        <property type="entry name" value="RasGAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143885">
        <property type="entry name" value="RGC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="3">
        <location>
            <begin position="5"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="2">
        <location>
            <begin position="760"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="642"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1397" mass="160467" checksum="44D3F96CFAF3611D" modified="2017-01-18" version="1" fragment="single">GYDYLCRIGEAKEWIEKCINEPIPSPTELATDALRDGVILAKLTQAFNPEIVRRIIPSGAKLQFKHTENINVFFHFLDFVKMPDLFRFELTDLYDKKNIPRVIFCIHALSFMLSHDSQAPLIEKLDGKLNFSDDEIKKSEHSLSGVSLPNFDSMNKQFGRELPKSPSKLTIPSLKENISTPLKSPAKVTSRFTNSPFFDQSEDTQQVPPKFSLTKKPSINVAKYSTPVEPVDLVEPVDDSFENSLVNGYNVSPSKNKRLSESEYLRNIIKFQAISRGSIFRYGMFVDRIILKSMSEDIVQMQSLVRRAITKRRYFTNTSSISNYEPEIVGLQSAIRAKLQKGRIYSVTKAQPDDKTVNLQSICRGKLIRDRLFDIKMALIRNEYRIIDVQAAARKRIVYDRVQKSVLSKEPIIKFQSQARRVLYSRKIKTSVSEAHSAEDVIAILQSVIRANRVRHEVDLKFTVLSNSTPQLLELQSIARGGFGRNRLNHILDVIDEQEPYLQELGALIKGKIVRRNVVADKAQLSRLSGSIVNVQSIVRGVLTRYGFDLFLDTLDMYTEETVGLQASIRGYLYRKSKNEVMEYYKQHLPAIIKIQSFIRGNYLGNAYKSLISMENPPLSVIKKFAYLLNNSDVDFEEEVNLTRIKEAINEKTASNESLEKHIGQLDLKIALLQKNKITLDELLLQKDTVLPNSDHNHSTVDVSSLNRKAKTRMELYEKFFYLLQTQPAYLTRLIQGDYRGVDVALKIFNNLRSEELPTREEYLFVSLIESTLKDFITKSHSIDDLQKTDKIGWQRLLKTFNETQKKPLVDMFGEMVENIMSDSQLDIESDPVLIYRIVHNTPRSIAVDKAIEDPDTKATFITNLQHLREFGSQIFRVLNDDYEKLPIHIRLLANIAYKASMKKYPTNLEQDHLANAGYVIINGFVKLILESPDIFKISPINVSHNARKNLNGISKLLTQLFTMKPFGSDSIYLQPLNKFINSSVDGVRSLIKSIIDVGEIDSVYNMTVYDDMTAHNRPKLTLRISDMLSIDELIRQEIKTVAPMKDDTLRDILHELKSNNANDISRISGLYTLSLNPSSIRSSTEETRIKALFMQAKRCVLYMIQVQDDKNDLYDLLRSKIDAIHEIKFKQIINNEKKDIQMNRASEYVNDGQGFLGDLSSITYHKLKQLSLERILELEAMGRLTRRDSFQILLNEIAYDIKTKHDQRISRKKQLSIAENTLIRLDEKEKFLQDQLNAYNHHIDKSMEELQSRPTRTKRIMPFTKQFFYERELKKSGKLPKFGAYKYSSKRLYEKGILVELRGLDQNLGSSGTSFFGGFNFPRIDFMFNCDKPGVFTISSKSGTLAVNGSLATLTIDELLEYQFENKSSIPLFDGMVKFDTNCLINFIFKKFYHSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVV9</accession>
    <name>A0A1E3NVV9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_71190</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036461.1">
        <property type="nucleotide sequence ID" value="XM_019184235.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVV9"/>
    <dbReference type="GeneID" id="30201481"/>
    <dbReference type="OrthoDB" id="3862662at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="5"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="108"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="61"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ57254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="825" mass="95447" checksum="934D817902A2A1EB" modified="2017-01-18" version="1">MKAPSCTSCRLRRRKCDKQTPICGSCKELGIPADLCTYKILKTNTKVKDLRTILDDSKLKNQKLITEKLVLKKALEDKKREIREQCTAIKRETGSDKHDQYEVNPANVGSVHHMSTPTSQISEVRSTSRSPFVVSRKKPHGDSISWASLMSMEPKMKVLLSQIDRVINEQKTQLESSKRELKDPLKKASKLSHLRTTGIAHALELDNTDICADIHELLEEIQTHLPEKKTWDRVLELQFHIVPPKYVGFLHLDHDTYQEILNRSVEFDNLGNPSLNITLPQEADKLRDLGFLISYLIFTAYHTTQVIGESFPMSPVMCSRYSETLLWYHRIMSVKLGNLEDAQITPEYLQALIYNEIFNRYTPHGEYIPRSYEDNAFCIRRLVSIGRVLYLNQNVDQVYMNRSEAYRSSLKSTWNFLAFFDILESLDNGFPPSIKPNELVRYQNFSDRYTESLIVLNKVLHTYIKLQCKEYNDAKFIQSIENDCIGELQELLATEYRPWDQDYQLFMSFTFEDSSRSREFTYESASFNMRFTIYSTIQIFYFLCFEKASKSEICSSKLARRLEILSLKYSYLLVLLINAYFEIYDKLITLPKSSENGVLEMMLATFPHLILALRKLQVCAGAKIFRSIPLRNVSAVKTFLNGTKIDEEAFLREVGEKKQSYKELFTIGELSDSSLDKDDDKIAAKYSPLMDFKFVIYVAGAITGELAERMFSEKSSLRMVKLNHVFFYLLKLSSFFMNCSFYSDGTPIENHKNYIKGLHRIKPQDSTSFSTEFDFQRLFEEAINTNSESFDFSGFFNISNGPFQNSDIDEFLSGYPSFHNDFGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVZ1</accession>
    <name>A0A1E3NVZ1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_36875</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035954.1">
        <property type="nucleotide sequence ID" value="XM_019182481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVZ1"/>
    <dbReference type="STRING" id="683960.A0A1E3NVZ1"/>
    <dbReference type="GeneID" id="30199727"/>
    <dbReference type="OrthoDB" id="5579281at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902657">
        <property type="term" value="P:protein localization to prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040345">
        <property type="entry name" value="Mug56/Spo71"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039486">
        <property type="entry name" value="Mug56/Spo71_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057379">
        <property type="entry name" value="PH_SPO71"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28076:SF1">
        <property type="entry name" value="PROSPORE MEMBRANE ADAPTER PROTEIN SPO71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28076">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN 71"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF15404">
        <property type="entry name" value="PH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH_SPO71"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="359"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="630"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ56747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="817" mass="95126" checksum="70C812B3AA604B9F" modified="2017-01-18" version="1">MENPKVRFQQSSTSTKPKGKSVSAQFLKPQPEENPIILPLDPRHERALNRIKAVASRTKRHVRNDLHNINQRRIFHKLLKNYKVGEIVKMERMLVLVKQAMDSSGMVLTTFTEVEPCDTRILERWKEYIVVARTTGDFDEPILIQFYTKREIPHDETSKHSDDDLDFTLNKCCLVNFYSSLDKTIAIVKKGKVFILRCQTPTSSIHWLTFLNETLGHSNPGKLIIQIPKLSVSINIRVTSEVYQKLTEEENAEYSSLIYKNEGYLVKELPILEYLVQKIKLNLVKSGYGSIVDDWNNKNLVLAFCWRHYDRLEWIFGETLSNLFWEQSMYSTHDLELRVSSHYPAEITNENNQKLEEPAPIEGFLARLSSRRGTHRNYIHKQIFKFSYFFTNNRLLFFSRSYKALPPIPEFEYLSDCKFLSNPENFEKIRHGIPPIYHHNPYRIDDAGHIDWLNPSISSEEFGNRDRFASYEYERRVASILRADAVIDMLEIVNVKAINADSLPVTVRAADIYAWEELTGKLKDESEEMKDSFFQIEFQNGSFFTLKAPSRTIRTEWVDRLNQLVTYWKLRVENDVEKIHEVKFSNINKLSIDDYMEANVGEATPKWENSRGLADPAIHNISSNAMFRPLVRCGLLYQKPKKHTVFKNYFVCLIPGFIILYKVFVRNLSGIALPTTHYKHYLTIPLDECYIYSGSTSGIDLLKRDSEIDNVNLGRNSLPRIYKDGWISSEDEASRCFTLWFGTKRAITGKMLMKSDEGNDFNEYGSQNPGIIKMVSRLGVTGRSVVFMCRSRQERDLWVNNIYTEVERFHKAEDVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW60</accession>
    <name>A0A1E3NW60_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_80699</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019185111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW60"/>
    <dbReference type="STRING" id="683960.A0A1E3NW60"/>
    <dbReference type="GeneID" id="30202357"/>
    <dbReference type="OrthoDB" id="3981267at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="405" mass="46076" checksum="D90BB3BB2B4C09DF" modified="2017-01-18" version="1">MVDESYLGNNMQLGEYDANNERISYMRTPIINNSKLWNSSYEPSPIAPNNLNSPAAANVTPNNITDSNYHESRSKLRQSFPLYSNITTGHEPNAVSTPILSYKHSHMQYNLNEQTASNNTPLLTAPVPPPGFFTEFDLNQQLTPNFTASGITFHKSQQQQQQGGVIPPEFLYSMPPQEQFPATFDPRSAANMPHKEKVNDWITKVPIYCLADGQLWYSECYPGVVPASSSISTEDESVDDLDIFNYNQRLLGNEFDYDEQLFVDNEDVLEFQARRITRYVKKIYKMDSSEHVVKGDKQYINNIDHTPLQLMLYDQSDSNIEITNTSYNLNLTDAFDQIHRKNLKYQYDEDGDLIEPDHKHVDLLKDHPPPTKSKAIFSEALAAMGAPRTRSTPRGISAGDSRYRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW71</accession>
    <name>A0A1E3NW71_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GID complex catalytic subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glucose-induced degradation protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_57746</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RMD5/GID2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036666.1">
        <property type="nucleotide sequence ID" value="XM_019182813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW71"/>
    <dbReference type="STRING" id="683960.A0A1E3NW71"/>
    <dbReference type="GeneID" id="30200059"/>
    <dbReference type="OrthoDB" id="1933281at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16652">
        <property type="entry name" value="dRING_Rmd5p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000143">
        <property type="entry name" value="Regulator of gluconeogenesis Rmd5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045098">
        <property type="entry name" value="Fyv10_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037683">
        <property type="entry name" value="Rmd5_dRing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044063">
        <property type="entry name" value="ZF_RING_GID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12170:SF3">
        <property type="entry name" value="GH10162P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12170">
        <property type="entry name" value="MACROPHAGE ERYTHROBLAST ATTACHER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51867">
        <property type="entry name" value="ZF_RING_GID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="CTLH" evidence="11">
        <location>
            <begin position="5"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="RING-Gid-type" evidence="12">
        <location>
            <begin position="185"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="RING-Gid-type" evidence="9">
        <location>
            <begin position="185"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00075398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00080744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ57459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="244" mass="28152" checksum="9933B3E3AE992D60" modified="2017-01-18" version="1">MNHILKKMLNERDLGDAIQWAIKNEAVLLTQMGSDLEFNLHKLQFLEYYNSGEIFKAYQYGKQWFPKFINTNSENLQSVSKLISSILFDSKDESSPYYKENQLSNSNFQEIGILFSKKFCSVIGFSFESSIFMILLCGYISFPTFLKFVKIKNLNNKLDWTSHNELPFEINLPDFLKKFHPIFICPVSKEETTMENPPMALPCHHIISKQSLNKLSRNGGSFKCPYCPTSSIPSKAKQVHFGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW92</accession>
    <name>A0A1E3NW92_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein bS6m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_34828</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036613.1">
        <property type="nucleotide sequence ID" value="XM_019182240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW92"/>
    <dbReference type="STRING" id="683960.A0A1E3NW92"/>
    <dbReference type="GeneID" id="30199486"/>
    <dbReference type="OrthoDB" id="10259681at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15465">
        <property type="entry name" value="bS6_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.60:FF:000007">
        <property type="entry name" value="37S ribosomal protein Mrp17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000529">
        <property type="entry name" value="Ribosomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035980">
        <property type="entry name" value="Ribosomal_bS6_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00166">
        <property type="entry name" value="S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS6M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01250">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54995">
        <property type="entry name" value="Ribosomal protein S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="115" mass="13198" checksum="25222D7FD3221ED3" modified="2017-01-18" version="1">MLYELIAIARVVNPIEHTREAKEVATTLGRLIIQNRGVVRKIQSMGNETLPKIMRKDQERHFQGHRFLMLFDSSAPVQSEILRTLKADPRIIRSYMQKVSDKNLDPGSSLDRAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWA2</accession>
    <name>A0A1E3NWA2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_96712</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036623.1">
        <property type="nucleotide sequence ID" value="XM_019186529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWA2"/>
    <dbReference type="GeneID" id="30203775"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="74" mass="8502" checksum="921CD73580CF21ED" modified="2017-01-18" version="1">MRSTKTSKCQRKNSNGDQILNLRWQKPKILSCRSFSGAHLEIPFALCRRSLTVTDLVLKGKHSSRKVNKHTSMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWL1</accession>
    <name>A0A1E3NWL1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">O-acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_36266</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Sterol O-acyltransferase that catalyzes the formation of stery esters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036279.1">
        <property type="nucleotide sequence ID" value="XM_019182413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWL1"/>
    <dbReference type="STRING" id="683960.A0A1E3NWL1"/>
    <dbReference type="GeneID" id="30199659"/>
    <dbReference type="OrthoDB" id="10039049at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034737">
        <property type="term" value="F:ergosterol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008204">
        <property type="term" value="P:ergosterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140042">
        <property type="term" value="P:lipid droplet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF23">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000439">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="265"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="407"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000439-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ57072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="427" mass="50644" checksum="545F8844D78EBAE8" modified="2017-01-18" version="1">MDHSATRTNRFAGFFVLFWLAVAVVVVDSVVNYIRAYGLESQIVTLLLTDLFDIAFMDLLMYLSIYFAFILHSFVAKGFLSWSKSGWIILSFYEFIFLVVFMSIAEHRNFPWIGKIFLFLHQLVLLMKMHSYGFYNGYLWRIYDELKFSRQFQKKNEDIEDDHVKNLINESIEFCEFELQSQSNIEPFPTNINLKNFFNYTMFPTLIYQIEYPRTERIRWSYVGEKLCAIFGIILLMIVIAQNYMYPLAIKALELRTLPLNERILHYPLLLLNIMPSFLLIYILVWYLIWDAILNCIAELSKFGDRDFYGDWWNCVSWDQFAREWNIPVHKFLLRHVYHSSISALRLSKTSATIFTFLLSSIVHEMAMYVLFHKMRGYLLLLQMFQLPLVAISKTKYLKERRTLGNIIFWVGIASGPSVMCTLYLTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWQ7</accession>
    <name>A0A1E3NWQ7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_35292</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036785.1">
        <property type="nucleotide sequence ID" value="XM_019182291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWQ7"/>
    <dbReference type="STRING" id="683960.A0A1E3NWQ7"/>
    <dbReference type="GeneID" id="30199537"/>
    <dbReference type="OrthoDB" id="3980796at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF11">
        <property type="entry name" value="ZINC FINGER PROTEIN YPR022C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="800" mass="93327" checksum="BC319431AC7828D1" modified="2017-01-18" version="1">MIKNVSNNSTAAVFKCPTCGKSYSRKDFLARHQLNHKNIRPFKCETCDLSFTRRDMLSKHCKSHSHQKMVEQKAKATTNNIEEDVDNNKVDQYQTSSEAVSPATFNEPNNENIDTNSLDESPKEFLSLLDMSGGFIDNLLWLFTDNFPEEMLNENNLVSHPESAVIDVDATTKFDIAPASYNVTDAHTHMFGSKRFKINETTRLKILEIFSDLESLKNVSLSRLDEYMDLYWFNFAQTFPIIHQVTFDPNKADIYLLISILIIGMAHTADRLEYELSIQINKNFRRRIKDVIDEDTQLSLPIMQALILHNFSAKNFGDTKLTQMSQIDHGSNLLYLKFSGFLENLSAEPVIHKSKDSTSEELYEQWQKWIYYETSKRTVFFEYICDTQHITFNRAELSQFDIKSELPCSDQVWNASTPEEFFAAYQIQPNSLVTRPKLNIHANDTTDNLQKHRTRPTRPVLGKWPTFLWTLKSFMTEYRENQREFSLNCYSLFSRYIILHSLIRVCWHARGHGLLDLGIISQNRLNEFFNKLELAFANWKGYFDLHIKLYDDQLNTVDPKKGQVLLNNYGPTNACWANLSFFYVGLFTLYADITSLSKFATEYNKPLSTKFHQDLKELEHERNIVLVEQWARSSNAEIAVIEACKYFTLVYKHEETINTYSHVPPSVLIATMIIWCFEKKNCFYDLERKLKIGSREQLNLKPSKYFDRRGDIIHSLAREQAHNYCELIAKQANDRDDDSQTDDDGKDVKSLKIQKIKEYKKRQEYTVGVVCYGLDLIRHCKWPHSIDIVEKLEHIVKSYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWT2</accession>
    <name>A0A1E3NWT2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_64818</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036677.1">
        <property type="nucleotide sequence ID" value="XM_019183814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWT2"/>
    <dbReference type="GeneID" id="30201060"/>
    <dbReference type="OrthoDB" id="3982939at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16448">
        <property type="entry name" value="RING-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133539" description="RING-type domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="113"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="176"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="296"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="386" mass="44794" checksum="BA180ED120380FD4" modified="2017-01-18" version="1">MRLGTLTIVVSGILLSTTLNPILGAPSAFPFFNYKTEPTNQQEIEEFFAKDDCTIYRKLGDIDWDLLTSEDLPPESYHPEFIAYVEKIKHLNGSSPCDSPRIGDDGKWMMRTFAFVVVGISVVFFSLFIAVALLEICALSFRRFRAKYRRLTRLVIRGDITKSQKFHDLFFSKDSLFFFSSCIISLTLGLPSIVIFLLTLFVVVTIEHIIGKQLFNDEENEAPAVIYVNGYNNQGIDRNKTFTRVTRKDVDSFVPAYNTPNARKFDYLIPELSFNKILKFKELMENYPEISKEPFCVVCLEEYLDDVNVLLMPCKHYCHYDCMEDMKYVTGRQNRIEGTSHLQRYSNVFNTRCPLCQLNLLRHFLFYKEYGLDPNEIEYLNTGYNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWX4</accession>
    <name>A0A1E3NWX4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_97044</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036855.1">
        <property type="nucleotide sequence ID" value="XM_019186555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWX4"/>
    <dbReference type="STRING" id="683960.A0A1E3NWX4"/>
    <dbReference type="GeneID" id="30203801"/>
    <dbReference type="OrthoDB" id="1703270at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11380">
        <property type="entry name" value="Ribosomal_S8e_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.168.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.168.20">
        <property type="entry name" value="Ribosomal protein S8e, subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001047">
        <property type="entry name" value="Ribosomal_eS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018283">
        <property type="entry name" value="Ribosomal_eS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042563">
        <property type="entry name" value="Ribosomal_protein_eS8_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_Se8/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00307">
        <property type="entry name" value="eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10394">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01193">
        <property type="entry name" value="RIBOSOMAL_S8E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="203" mass="22686" checksum="5728E36F5FFAB708" modified="2017-01-18" version="1">MGISRDSRHKRAATGAKRAQFRKKRKFELGRQGANTKIGTKRIHSVRTRGGNQKFRALRVETGNFSWASEGVARKTRIVGVVYHPSNNELVRTNTLTKSAIVQIDAAPFKQWYETHYGASLGKKKGQAKAADEEEVKKSRKLERKLASRSQQAKIEHAVDAQFGAGRLYAAISSRPGQSGRADGYILEGEELAFYLRRLSAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX59</accession>
    <name>A0A1E3NX59_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP synthase subunit delta, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">F-ATPase delta subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_97684</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase epsilon chain family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036374.1">
        <property type="nucleotide sequence ID" value="XM_019186605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX59"/>
    <dbReference type="STRING" id="683960.A0A1E3NX59"/>
    <dbReference type="GeneID" id="30203851"/>
    <dbReference type="OrthoDB" id="270171at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12152">
        <property type="entry name" value="F1-ATPase_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.15.10:FF:000003">
        <property type="entry name" value="ATP synthase subunit delta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.15.10">
        <property type="entry name" value="F0F1 ATP synthase delta/epsilon subunit, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00530">
        <property type="entry name" value="ATP_synth_epsil_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001469">
        <property type="entry name" value="ATP_synth_F1_dsu/esu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020546">
        <property type="entry name" value="ATP_synth_F1_dsu/esu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048938">
        <property type="entry name" value="ATPD_C_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036771">
        <property type="entry name" value="ATPsynth_dsu/esu_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01216">
        <property type="entry name" value="ATP_synt_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13822">
        <property type="entry name" value="ATP SYNTHASE DELTA/EPSILON CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13822:SF7">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT DELTA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02823">
        <property type="entry name" value="ATP-synt_DE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21334">
        <property type="entry name" value="ATPD_C_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51344">
        <property type="entry name" value="Epsilon subunit of F1F0-ATP synthase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="11" id="KW-0139">CF(1)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATP synthase F1 complex delta/epsilon subunit N-terminal" evidence="15">
        <location>
            <begin position="32"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="F1F0-ATP synthase subunit delta C-terminal" evidence="16">
        <location>
            <begin position="119"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF21334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ57167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="161" mass="16952" checksum="E8D668D503BA8342" modified="2017-01-18" version="1">MFRSNAFKSVARQTQLIARRTYADAASATDVLKLSLALPHETLFSSSEVTQVNVPGVNGDFGILANHVPIIEQLRPGVVEIIESSGTTKQFFVSGGFATVQPGSTLSITAIEAFEPSAFSAETIKSQLAEAQKNVTSSDEAVAAVAGIQIEVLEALQSVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX63</accession>
    <name>A0A1E3NX63_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ArsA/GET3 Anion-transporting ATPase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_85699</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3. Within the complex, GET1 and GET2 form a heterotetramer which is stabilized by phosphatidylinositol binding and which binds to the GET3 homodimer. Interacts with the chloride channel protein GEF1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Golgi apparatus</location>
        </subcellularLocation>
        <text evidence="11">GET1 and GET2 are required for targeting GET3 to the endoplasmic reticulum.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the arsA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036767.1">
        <property type="nucleotide sequence ID" value="XM_019185934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX63"/>
    <dbReference type="STRING" id="683960.A0A1E3NX63"/>
    <dbReference type="GeneID" id="30203180"/>
    <dbReference type="OrthoDB" id="1770at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02035">
        <property type="entry name" value="ArsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001359">
        <property type="entry name" value="ATPase GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03112">
        <property type="entry name" value="Asna1_Get3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027542">
        <property type="entry name" value="ATPase_ArsA/GET3_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="ARSENICAL PUMP-DRIVING ATPASE ARSENITE-TRANSLOCATING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803:SF3">
        <property type="entry name" value="ATPASE GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="12">
        <location>
            <begin position="22"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ57560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="343" mass="38119" checksum="27E4442310764766" modified="2017-01-18" version="1">MSDDFEAPDPNIKSIISHDTLKWIFVGGKGGVGKTTSSSSISIQLALNNPTKQYLLISTDPAHNLSDAFGEKFGKDARKVTGMNNLSCMEIDPSSTIQDLSANFDTAQSNDPLKNMMSDLTSSIPGIDEALSFMEVLKHIKNQSNKPDQDGDIETIQYDTIIFDTAPTGHTLRFLQLPQTFSKLLAKFDELSGRFGGLLNNLGGAQMGDINSKMSELKELTALINEQFTDPEITTFVVVCIAEFLSLYESERLIQELDSYGIDSSTIIVNQLLFTQNDDPCKRCQARSKMQNKYLNEMNELYEDFHLVKVPLVNTEVRGLEKLKKFSKFLVDPYVPGGSVDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXF8</accession>
    <name>A0A1E3NXF8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_33608</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037088.1">
        <property type="nucleotide sequence ID" value="XM_019182090.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="30199336"/>
    <dbReference type="OrthoDB" id="2018507at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967:SF65">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE AKL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="17"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="301" mass="34595" checksum="6334DA268589176A" modified="2017-01-18" version="1" fragment="single">MEKLSPGTKLQVGSHETEIVKYLAEGGFAHIYTVKQTPVEDGLEIACLKRVIVKTKPELNLLRKEVDTMKKLANNKYVVKYYDSNAQKLEDGTYEVLLLMEYCPNKSLLDLMNQHLKTKLSTDQILKIMFEISVAVSSMHYMNLIHRDLKVENVLINANNDFKLADFGSTSGFIAPPKSQEEFQAIAHDILYQTTPQYRAPEMIDLYKNVSIDYKSDIWALGVFLYKLMYYTTPFEAQGELAILHGFFQFPPAPKYPSRLKNLIIIMLQVDVNLRPNIYQVVYELCDILGKEVPIKDYYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXP4</accession>
    <name>A0A1E3NXP4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_80788</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036659.1">
        <property type="nucleotide sequence ID" value="XM_019185123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXP4"/>
    <dbReference type="STRING" id="683960.A0A1E3NXP4"/>
    <dbReference type="GeneID" id="30202369"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05471">
        <property type="entry name" value="pepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034164">
        <property type="entry name" value="Pepsin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133556" description="Peptidase A1 domain-containing protein" evidence="4">
        <location>
            <begin position="23"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="5">
        <location>
            <begin position="53"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="447"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="602"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="680"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="602"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="680"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="691"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="717"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ57452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="793" mass="82798" checksum="63B33618A4D46678" modified="2017-01-18" version="1">MKLGYNQLVVLITATLLGNASADILKVNLGGDKLVKRGFNQSSVEPITGKHIFEGKLEVGTPGQEVTVLFDTGSYTFALNYKDSEFCKQNGCSQYGSYDPSKSSSSVNTTDPYTASYLDGSSGGGHYFNDTLEIAGFTIDDYKFGLLEDTNSDSNIVGMALIAGDDKAPVLYNFLAHNGFTSRKVFSTYYSENSGAVVFGGLDQSKYSGTLEKIPVLAYNMYIVYLTGVSTDCGVVNKNGYPVILDTGSDGNVIPDELYHQLHQAIGGKVTKVPIQSYAEGPTGSIDSNYVLKCADAKPVQFDFSGKQVSYPPQHYLISLYNGDSPLTDSDGDTLCTTNFFNGGKSSDQSQFILGAQFLRNLLVVWDVDNAEVSIAQGASSGSEDLVEITGDVPKAVAAKDYNSPNSATTDTYYPSPSTESLSVTTILTSGYYSGSFQTAACTKASSSSSASSSSTTTSSLSSTSSSLSASSSSKFSSTIYSSAYSTVSSATCLQCSVSSNGSSTIYSSTYSTVSSPTCFSCAVVSSSSSSSVVLPSSVSTSGELTSVSSATASSVSSVSSEPVSASVSTYYQQQNSTIIKTITSCSDNACSKITTTSVFVPSSTQESASKTETIQSSLETPSLSTVQTSSAETTISKPSTIEAKQPSQSSELEIVSTTSSATANYTSDGTSQTELIPASSSVTTLDSQPSTTSSETTVVSSSIPIPSAESTTTNAPSSITQSITSTPIPSSSLVQSISSDEDTTVTFQTYVTVSQQSTSSLYNVSSVAISTFDAGANLVEVPFLFKIFSSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXX3</accession>
    <name>A0A1E3NXX3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nop domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_33636</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRP31 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037152.1">
        <property type="nucleotide sequence ID" value="XM_019182096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXX3"/>
    <dbReference type="STRING" id="683960.A0A1E3NXX3"/>
    <dbReference type="GeneID" id="30199342"/>
    <dbReference type="OrthoDB" id="4771285at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000002">
        <property type="entry name" value="U4/U6 small nuclear ribonucleoprotein Prp31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027105">
        <property type="entry name" value="Prp31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019175">
        <property type="entry name" value="Prp31_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13904">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13904:SF0">
        <property type="entry name" value="U4_U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09785">
        <property type="entry name" value="Prp31_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Nop" evidence="10">
        <location>
            <begin position="190"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="305"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="325"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ57945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="436" mass="48813" checksum="9E3DEC38806D21D5" modified="2017-01-18" version="1">MDKVTSQLHELVDSGKEVTTSDLLSRIDLSSIKDVTKISKLSHKVDPIIATIEKYTSQKVSKKEEYNFLITVNDLSVEITNEISLVHDFIKAHYRKRFPELESLIPNAIEYSKIVKVLQNNLNIDAENLSFLSKEKTLVLTMSSIQAKDASENIKEDELEKIVGACDLLLKLEDSRIKISDYVASRLSVFAPNLSATVGSYTAAQLMSVLGGLKGLAQTPSCNIASLGNKRAVGIGLGHSGVRQQGYLYYSDLIQSVYPDLRKQAMRIVSGKIILAARVDFSSSIPDGSQGQKWRRDIEEKLEKLAEPPENKAPKALPAPIDKSSKKRAGRKYRKMKQRFELSELRKAQNRMEFGKQENTVTDGFGEEIGLGMTGSLSNIPINTNTRAKVSKNMKNRLEKATQDSEFFNQSLLNFGEETKNDHRIQDGKETKRIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY22</accession>
    <name>A0A1E3NY22_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor SLT11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_16465</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in pre-mRNA splicing. Facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome. Binds to RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLT11 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037107.1">
        <property type="nucleotide sequence ID" value="XM_019180693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY22"/>
    <dbReference type="STRING" id="683960.A0A1E3NY22"/>
    <dbReference type="GeneID" id="30197939"/>
    <dbReference type="OrthoDB" id="10259600at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12265">
        <property type="entry name" value="RRM_SLT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034356">
        <property type="entry name" value="Slt11_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048995">
        <property type="entry name" value="STL11/RBM22-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF6">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21369">
        <property type="entry name" value="STL11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="182"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ57900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="287" mass="31990" checksum="665F341B131C40BF" modified="2017-01-18" version="1" fragment="single">AVCETCLGPNPYLRMMREREALECKLCTRPFTVFKWAPKKNGAFKKTIICLTCSRQRNCCQSCLMDLTYGIPIQLRDAALKMAGVQGITGDEPKNEISKLYVANNSQQFQTIGGASVTSNAERAKEILTKLSLALKNNKNPKKLNDKSDQLPSHISKIDVTKIISKLPFNGSLEPPKDETITTLFIFGIDESLPEYKITDYFEQFGKIKSFDCQHKARAGFITFQTRDNAEKATQSITSPQPQAPGLLIIENIPLRITWGKERPLGVSNAEKIKVGSVVGKILKKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYF8</accession>
    <name>A0A1E3NYF8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">REJ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_85261</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037360.1">
        <property type="nucleotide sequence ID" value="XM_019185866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYF8"/>
    <dbReference type="GeneID" id="30203112"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133566" description="REJ domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="90" mass="9494" checksum="82C3041007A4C814" modified="2017-01-18" version="1">MLFSVYFTILLSTIIAAVNGSPISLPSPSPSSTSVSTKPSTSASLTLSSLSTTTTLQPTKFPTLSIQQPVLSSNSNQFFTAVVIDKRQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYI1</accession>
    <name>A0A1E3NYI1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_34498</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the laat-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037476.1">
        <property type="nucleotide sequence ID" value="XM_019182192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYI1"/>
    <dbReference type="GeneID" id="30199438"/>
    <dbReference type="OrthoDB" id="8048523at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015101">
        <property type="term" value="F:organic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034488">
        <property type="term" value="P:basic amino acid transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034490">
        <property type="term" value="P:basic amino acid transmembrane import into vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000011">
        <property type="entry name" value="PQ loop repeat protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000012">
        <property type="entry name" value="Vacuolar membrane PQ loop repeat protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF35">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER YPQ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="203"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="309" mass="34524" checksum="A9B70D9466EE1004" modified="2017-01-18" version="1">MTNSTHASPHFSDVILDNKAISGITGSISISFWIIVFVPQIYENYVRKSSEGLSLLFVVLWLAGDVFNVLGAILQHVLPTMIILAIYYTLADLILLGQIFLYGDGSQVDPVHLSPANPLSENIFDEVLGDQESQSTTSIQYSQLEQGGVSNTKQSNKKSRKRTLIESSLIFFVILSGIIGWYISYNPDQDQDGDDDDELKFDFLAQFFGWLSAILYLGSRMPQILLNFKRKSCEGISFLFFLCACLGNLTYVISILSISVDPYFLLVQSSWLAGSIGTLSLDFVIFVQFFLYNRDGEDSDDSDIDEITD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYJ5</accession>
    <name>A0A1E3NYJ5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP synthase subunit 5, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_34688</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase delta chain family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037400.1">
        <property type="nucleotide sequence ID" value="XM_019182217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYJ5"/>
    <dbReference type="STRING" id="683960.A0A1E3NYJ5"/>
    <dbReference type="GeneID" id="30199463"/>
    <dbReference type="OrthoDB" id="1262810at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.20">
        <property type="entry name" value="N-terminal domain of the delta subunit of the F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01416">
        <property type="entry name" value="ATP_synth_delta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026015">
        <property type="entry name" value="ATP_synth_OSCP/delta_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020781">
        <property type="entry name" value="ATPase_OSCP/d_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000711">
        <property type="entry name" value="ATPase_OSCP/dsu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01145">
        <property type="entry name" value="ATP_synt_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11910">
        <property type="entry name" value="ATP SYNTHASE DELTA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00213">
        <property type="entry name" value="OSCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00125">
        <property type="entry name" value="ATPASEDELTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47928">
        <property type="entry name" value="N-terminal domain of the delta subunit of the F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00389">
        <property type="entry name" value="ATPASE_DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="206" mass="21858" checksum="4AB1012EDB8A78E7" modified="2017-01-18" version="1">MASRIFSRTLATAAASVKPPVQLFGLDGTYATALYTAAAKSTSIESAAKSLDSLKAKISKDSKIGEFLSNPALSSEDRAVVVDTLAKSTPSIDASVSNLLKVLGENNRLNLLEQVFTQFKVLTDAHHGIVEAIVTSSTKLDSKILKKIENSISKSKFVGSGKTLRLTNDVNPDILGGLIVEVGDRTVDLSIASKIARLNQTLKEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYR5</accession>
    <name>A0A1E3NYR5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_80307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037357.1">
        <property type="nucleotide sequence ID" value="XM_019185037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYR5"/>
    <dbReference type="GeneID" id="30202283"/>
    <dbReference type="OrthoDB" id="10510717at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133599" evidence="1">
        <location>
            <begin position="21"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="247" mass="26867" checksum="C2BC9CC14226B145" modified="2017-01-18" version="1">MFYKIITGLVLLLCASLSLADGSQNIGSLQVTPLDYDKYAETIDLKLLRFFDSNEVFFYDSEAKTYDSATQTVRNASILSTGQFTIGQEGKDYYLTINPVSGVVSVSKDSKTSNGLFYINDNYLYYQYHKLFVACKSTPDAHNYTLTWRGASSPAICQNDWLLIYLKPYGSSNGTAISSYYPNEYAASIASTATDNTDTSTVTAPVTTGSSKASANGANAHLVGIESGVSRYSGLSVLIVVLISFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYY3</accession>
    <name>A0A1E3NYY3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_80488</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037550.1">
        <property type="nucleotide sequence ID" value="XM_019185072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYY3"/>
    <dbReference type="STRING" id="683960.A0A1E3NYY3"/>
    <dbReference type="GeneID" id="30202318"/>
    <dbReference type="OrthoDB" id="5325318at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF4">
        <property type="entry name" value="CHAIN DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G08710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="255" mass="27542" checksum="854D0B9ABA45C630" modified="2017-01-18" version="1">MTFNKFDIKGRTALVTGGGRGIGLACAQALIESGCNCAIFDVIDSTIDVEELAKTNDVKVKHYICDISDEKALREGFAKFIQDFEGKLDICVPCAGVNKNLPFLDTTFEEFDQLTNINSRGAYFSAQLAAKQMIANKTTKGSIVFIASMGSYIAIRSQNSSAYCATKGAVRSMVAPISAELQKYGIRANSVSPGYIKTAMTAPFPDLIESWKDLTMNERVGDPEDIQGACVFLASDASTYMSGEDIIVDAGVTKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ06</accession>
    <name>A0A1E3NZ06_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription initiation factor IIA large subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_64494</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIA subunit 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037562.1">
        <property type="nucleotide sequence ID" value="XM_019183748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ06"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ06"/>
    <dbReference type="GeneID" id="30200994"/>
    <dbReference type="OrthoDB" id="6275927at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000979">
        <property type="term" value="F:RNA polymerase II core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07976">
        <property type="entry name" value="TFIIA_alpha_beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.100:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004855">
        <property type="entry name" value="TFIIA_asu/bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03153">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01371">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47396">
        <property type="entry name" value="Transcription factor IIA (TFIIA), alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="67"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="128"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="67"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="205" mass="22731" checksum="91DE1962B4E43BDD" modified="2017-01-18" version="1">MSNTEAARIYESIIDDVINDSRQDFEDSGIDEATLQDLRTIWRDNLTQTRVANFSWAPDAIVNNGLSTDPQNITEASSITSDVKKENSAVPIPQESTTGNFELELQGSNGDIAKSLQKQKENIEKKERTLASLDSDDINSDLDDSDEEDLGSDEDGDDAEGMIILCLYEKVLRVKNKWKCNLKDGVANINGKDYAFAKGTGESEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZB7</accession>
    <name>A0A1E3NZB7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">methylated diphthine methylhydrolase</fullName>
            <ecNumber evidence="6">3.1.1.97</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_96038</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>diphthine methyl ester-[translation elongation factor 2] + H2O = diphthine-[translation elongation factor 2] + methanol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42656"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="EC" id="3.1.1.97"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DPH7 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.97" evidence="6"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037571.1">
        <property type="nucleotide sequence ID" value="XM_019186510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZB7"/>
    <dbReference type="STRING" id="683960.A0A1E3NZB7"/>
    <dbReference type="GeneID" id="30203756"/>
    <dbReference type="OrthoDB" id="1930760at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061685">
        <property type="term" value="F:diphthine methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052415">
        <property type="entry name" value="Diphthine_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042:SF1">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="237" mass="26266" checksum="1F71521B2B6C9F0C" modified="2017-01-18" version="1">MFSQVHQDRILLTCTDGYAALVSLTQTSVSEPQFFGTQHDLECWTGAFGNMAELSNLVFTGGDDGSLIAHDTREPDSLSVFHARRIHEAGVVAIQTSTEGDPIRDGTGDWFSDKPYTLWTGSYDDNLRSLDLRVVPNVGIVSGIIPKVQEKVKLGGGVWRFTPSTIKNDNRLLTCCMYNGARIIGAKGDSEPIVERFFKQEHESMVYGCDWSPKGDQVATCSFYDCIVQVWSPDELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZF3</accession>
    <name>A0A1E3NZF3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_80223</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037269.1">
        <property type="nucleotide sequence ID" value="XM_019185021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZF3"/>
    <dbReference type="STRING" id="683960.A0A1E3NZF3"/>
    <dbReference type="GeneID" id="30202267"/>
    <dbReference type="OrthoDB" id="2449818at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.190.20:FF:000006">
        <property type="entry name" value="Ribosomal protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.790:FF:000002">
        <property type="entry name" value="Ribosomal protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.190.20:FF:000009">
        <property type="entry name" value="Ribosomal protein L10a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_uL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_uL1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ58062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="217" mass="24389" checksum="73B8BC28A0D2F059" modified="2017-01-18" version="1">MSKITNAQVRTHVKDILEYSNTTKKRNFLETVELQVGLKNYDPQRDKRFSGTLKLPQVPRPNMSICIFGDAFDVDRAKSIGADAMSVDDLKKLNKNKKLIKKLAKKYNAFVASDVLIKQVPRLLGPQLSKAGKFPTPVSHNDDLYAKVQEVKSTIKFQLKKVLCLAVAVGNVEMEEDVLVNQILMATNFLVSLLKKNWQNVGSLVIKSSMGPSHRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZJ2</accession>
    <name>A0A1E3NZJ2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_64604</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037675.1">
        <property type="nucleotide sequence ID" value="XM_019183770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZJ2"/>
    <dbReference type="GeneID" id="30201016"/>
    <dbReference type="OrthoDB" id="10592719at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133576" evidence="2">
        <location>
            <begin position="21"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="288" mass="30905" checksum="B6CE29E9042D49EE" modified="2017-01-18" version="1">MRFSTFVIFTAAVASSFVAGSDDDDDHKWVTYTRNLGSGRKLTLTRYQGSVPLVGSEYETKTIVVSNDDGSPRYTETVTGRREAVEERAATSEESSDPDWVTYTKNLPDTTVTVTRYQGSTPLTGTEYQTRTIVVSNEDGSPKYTEVITGRRDPATETAKASAASEESSSDPEWVTYTKSLADKTIVVTRYQGSTPLSGTEYQTRTIVVSNEDGSPKYTEVITGRRDPSTETEVAQSSSSKAEAKSSATSAESESSSSGRVSTYTGAAKTVGLSFGALAFGGVAAFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZQ4</accession>
    <name>A0A1E3NZQ4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-beta hydroxysteroid dehydrogenase/isomerase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_93029</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037792.1">
        <property type="nucleotide sequence ID" value="XM_019186393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZQ4"/>
    <dbReference type="STRING" id="683960.A0A1E3NZQ4"/>
    <dbReference type="GeneID" id="30203639"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002225">
        <property type="entry name" value="3Beta_OHSteriod_DH/Estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01073">
        <property type="entry name" value="3Beta_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133601" description="3-beta hydroxysteroid dehydrogenase/isomerase domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="3-beta hydroxysteroid dehydrogenase/isomerase" evidence="4">
        <location>
            <begin position="5"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ58585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="215" mass="24065" checksum="E9EB5E8EEA5B0081" modified="2017-01-18" version="1" fragment="single">MAKVLVTGASGFLALHIIGQLLSEGYSVIGTVRSQSKIEKLMSQFKTKYPEAVANLQFEIVPDIQLEGVFDKLFIEHKDIDYVLHTASPFSFGLEGSFDDVYLKPAVNGTLNVLKSIKNFASQVKKVVITSSMAAIDNMGDHELIHTEDIWNPLEWEQVNHEQLAYVASKKYAEKSAWDFIKDNAQQINFKLVTITPPFIFGPQFFDEDASSSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P002</accession>
    <name>A0A1E3P002_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Ferulic acid decarboxylase 1</fullName>
            <ecNumber evidence="10 12">4.1.1.102</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Phenacrylate decarboxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">FDC1</name>
        <name evidence="16" type="ORF">WICANDRAFT_93013</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the reversible decarboxylation of aromatic carboxylic acids like ferulic acid, p-coumaric acid or cinnamic acid, producing the corresponding vinyl derivatives 4-vinylphenol, 4-vinylguaiacol, and styrene, respectively, which play the role of aroma metabolites.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(E)-4-coumarate + H(+) = 4-vinylphenol + CO2</text>
            <dbReference type="Rhea" id="RHEA:33227"/>
            <dbReference type="ChEBI" id="CHEBI:1883"/>
            <dbReference type="ChEBI" id="CHEBI:12876"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="EC" id="4.1.1.102"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>(E)-cinnamate + H(+) = styrene + CO2</text>
            <dbReference type="Rhea" id="RHEA:46920"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15669"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:27452"/>
            <dbReference type="EC" id="4.1.1.102"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(E)-ferulate + H(+) = 2-methoxy-4-vinylphenol + CO2</text>
            <dbReference type="Rhea" id="RHEA:33807"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29749"/>
            <dbReference type="ChEBI" id="CHEBI:42438"/>
            <dbReference type="EC" id="4.1.1.102"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>prenylated FMN</name>
            <dbReference type="ChEBI" id="CHEBI:87746"/>
        </cofactor>
        <text evidence="12">Binds 1 prenylated FMN per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. May form higher order oligomers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the UbiD family. UbiD-like/FDC subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.102" evidence="10 12"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037777.1">
        <property type="nucleotide sequence ID" value="XM_019186392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P002"/>
    <dbReference type="SMR" id="A0A1E3P002"/>
    <dbReference type="STRING" id="683960.A0A1E3P002"/>
    <dbReference type="GeneID" id="30203638"/>
    <dbReference type="OrthoDB" id="4878259at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046281">
        <property type="term" value="P:cinnamic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033494">
        <property type="term" value="P:ferulate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1670.10:FF:000004">
        <property type="entry name" value="Ferulic acid decarboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1670.10">
        <property type="entry name" value="UbiD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01983">
        <property type="entry name" value="UbiD_FDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032903">
        <property type="entry name" value="FDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002830">
        <property type="entry name" value="UbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049381">
        <property type="entry name" value="UbiD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049383">
        <property type="entry name" value="UbiD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048304">
        <property type="entry name" value="UbiD_Rift_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00148">
        <property type="entry name" value="UbiD family decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108">
        <property type="entry name" value="3-OCTAPRENYL-4-HYDROXYBENZOATE CARBOXY-LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108:SF17">
        <property type="entry name" value="FERULIC ACID DECARBOXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01977">
        <property type="entry name" value="UbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20696">
        <property type="entry name" value="UbiD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20695">
        <property type="entry name" value="UbiD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143968">
        <property type="entry name" value="UbiD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 12" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="8 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 12" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like N-terminal" evidence="14">
        <location>
            <begin position="30"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like Rift-related" evidence="13">
        <location>
            <begin position="132"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like C-terminal" evidence="15">
        <location>
            <begin position="343"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="186"/>
            <end position="191"/>
        </location>
        <ligand>
            <name>prenyl-FMN</name>
            <dbReference type="ChEBI" id="CHEBI:87746"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="208"/>
            <end position="209"/>
        </location>
        <ligand>
            <name>prenyl-FMN</name>
            <dbReference type="ChEBI" id="CHEBI:87746"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>prenyl-FMN</name>
            <dbReference type="ChEBI" id="CHEBI:87746"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>prenyl-FMN</name>
            <dbReference type="ChEBI" id="CHEBI:87746"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF20695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF20696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ58570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="518" mass="58065" checksum="8ED19D0BF34D5226" modified="2017-01-18" version="1">MSKVITRTFHNTARASNGTLKPALRFRDFIQQLKNEGDLVEIEQEIDPILEVGAITRKVYEEKLPAPLFNNLKGHQENLFRILGAPGGLRSGKEDSHARISHHLGLPSNTHVKNIIDYLLEAKTKKPIPPHEVSNKDAPVKENLLQGDDIDLTKLPNPLLHPNDGGKYLQTYGMWVLQTPDKKWTNWSIARGMVVDERHISGLVINPQHIKVVADEWAKIGKGDKIPFALAFGVPPAAILVSSMPIPEGVNESDYIGALLGESLPVVKAETNELMVPATSEIVMEGTLDLNHLTPEGPFGEMHGYVFKGQGHPCPTYTVDSISYRNNSILPVSNPGLAPDETHTFIGTLVSAEAKQIAIEHDLPIIDVFSPYESQALWLVFKVDLKKLQALGTTPESFAKKIGDIYYNHKVASIIHEIILVGDDIDIFDFRKVIWAYTTRHTPHDDQYYFPDVRPFALAPFVSQSPRIKSMKGGKVVTNCIFPQQYEKDIEFTTCEYDTYDTEIKEKVEQNWKAYGYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P054</accession>
    <name>A0A1E3P054_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_32705</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038026.1">
        <property type="nucleotide sequence ID" value="XM_019181998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P054"/>
    <dbReference type="STRING" id="683960.A0A1E3P054"/>
    <dbReference type="GeneID" id="30199244"/>
    <dbReference type="OrthoDB" id="1650at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071034">
        <property type="term" value="P:CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22525">
        <property type="entry name" value="KH-I_Rrp4_eukar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05789">
        <property type="entry name" value="S1_Rrp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000063">
        <property type="entry name" value="Exosome complex component RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000038">
        <property type="entry name" value="Exosome complex component RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025721">
        <property type="entry name" value="Exosome_cplx_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026699">
        <property type="entry name" value="Exosome_RNA_bind1/RRP40/RRP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048565">
        <property type="entry name" value="S1_RRP4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321:SF4">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321">
        <property type="entry name" value="PNAS-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14382">
        <property type="entry name" value="ECR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15985">
        <property type="entry name" value="KH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21266">
        <property type="entry name" value="S1_RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0271">Exosome</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exosome complex component N-terminal" evidence="7">
        <location>
            <begin position="46"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="RRP4 S1" evidence="9">
        <location>
            <begin position="95"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="189"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF15985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="350" mass="38739" checksum="F096EF0B3CEA55D1" modified="2017-01-18" version="1">MDILSISKSSQLTYNDAEESDSDIELLSDHEEQKVQTDENGLKKSVVTPGELVTDDPIWMRGHGTYYLDNHTYSSVAGTITRTNRLLSVTPIRGRYQPETGDHVVGRITEVGQRRWKVDIGAKQDAVLMLGSVNLPGGVLRRKSESDELQMRNFLKEGDLLNAEVQSLYQDGSASLHTRSLKYGKLRNGIFLKVPSSLIVRAKNHSHQLPGNVSIIIGVNGYIWLRKTTEDLLTNNNSSTIGKNATAVNSKGQYNPGAGSVSITRLEEESSWEIYSDTNDQISSSIRNTITRYSNCIKALAFCELGITESRIVSAYEASMQYENAGQLVDIEVMESLGQGIITAEKYRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0A0</accession>
    <name>A0A1E3P0A0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">5'-deoxynucleotidase</fullName>
            <ecNumber evidence="8">3.1.3.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_34134</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the dephosphorylation of the nucleoside 5'-monophosphates deoxyadenosine monophosphate (dAMP), deoxycytidine monophosphate (dCMP), deoxyguanosine monophosphate (dGMP) and deoxythymidine monophosphate (dTMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'-deoxyribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18274"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65317"/>
            <dbReference type="EC" id="3.1.3.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the HDDC2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.89" evidence="8"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037579.1">
        <property type="nucleotide sequence ID" value="XM_019182151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0A0"/>
    <dbReference type="STRING" id="683960.A0A1E3P0A0"/>
    <dbReference type="GeneID" id="30199397"/>
    <dbReference type="OrthoDB" id="10254258at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002953">
        <property type="term" value="F:5'-deoxynucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009159">
        <property type="term" value="P:deoxyribonucleoside monophosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000011">
        <property type="entry name" value="HD domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039356">
        <property type="entry name" value="YfbR/HDDC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845:SF13">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13023">
        <property type="entry name" value="HD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0170">Cobalt</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="13">
        <location>
            <begin position="59"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ58372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="223" mass="25955" checksum="A3A923AD743FAD9E" modified="2017-01-18" version="1">MTASETQWTVESAIPQNVKDLLTPEAGETKINYVLVFLQIVSLLKSQRRTGWVDHGIPDCESISDHMYRMGVTSLLIKNKDVDTNKCVKIAIVHDIAESLVGDITPFDGVTKEEKHRRELDTIHYLSDLVKKYDEENGKEILELWLDYENIRTLEARYVKDIDKFEMLLQGFEYEKKFQGTKRLDQFWGARSSVKTDEVKGWTDDLFAKREQFWKKIEASKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0Z0</accession>
    <name>A0A1E3P0Z0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glycoside hydrolase family 13 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">AAM1</name>
        <name evidence="6" type="ORF">WICANDRAFT_93717</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038200.1">
        <property type="nucleotide sequence ID" value="XM_019186429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0Z0"/>
    <dbReference type="STRING" id="683960.A0A1E3P0Z0"/>
    <dbReference type="GeneID" id="30203675"/>
    <dbReference type="OrthoDB" id="1740265at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004556">
        <property type="term" value="F:alpha-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033934">
        <property type="term" value="F:glucan 1,4-alpha-maltotriohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004574">
        <property type="term" value="F:oligo-1,6-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004575">
        <property type="term" value="F:sucrose alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000025">
        <property type="term" value="P:maltose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005987">
        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11333">
        <property type="entry name" value="AmyAc_SI_OligoGlu_DGase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000064">
        <property type="entry name" value="Oligo-1,6-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.400.10:FF:000004">
        <property type="entry name" value="Oligo-1,6-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000087">
        <property type="entry name" value="Oligo-1,6-glucosidase IMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000007">
        <property type="entry name" value="Sucrose isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="entry name" value="Oligo-1,6-glucosidase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF179">
        <property type="entry name" value="NEUTRAL AND BASIC AMINO ACID TRANSPORT PROTEIN RBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0462">Maltose metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="5">
        <location>
            <begin position="16"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="570" mass="66497" checksum="74C1DE4DC07DB18C" modified="2017-01-18" version="1">MTISTYPWWKDSTVYQIWPASYKDSNGDGFGDIPGIISTLDHVKNLGTSVIWLSPHYASPQDDMGYDISDYEAIYEKYGTMEDMDKMIDEVHKRGMKLIFDLVINHTSSEHAWFKESRSSKDSAKRDWYIWKKPRYDEQGNRHPPNNWMSYFSGPAWEYDEATDEYYLCLFAKSQPDLNWENEATRDAIYKSALEFWFKKGVDGFRIDTAGMYSKDQRFLDAPIVFPDQEWQPSNDYTTNGPRIHEFHKEMFAKVTSNYDVMTVGEVGHCSKEEALKYVSAKEKEMNMMFLFDIVEAGCDTKDRFRYHGFKLKDVKDAIFNQCDFIKGTDAWSTVFIENHDQPRSVTRFGNTDNKLLLFKSAKLLSILQTTLTGTLFIYQGQEIGMTNLPRSWDISEYKDINTINYWDAFKKKYPNDPKKAEELMDLINLLARDHARSPVQWDDTLHGGFTTGTPWTRVNDNYPEINVKAQTGDPNSVLSFWQKSIKVRQFYKDVFIHGDFEILDYENEKTFTYVKTSPDSKAYIVLNFSNENVAFKPLIDGEFKLINSNVDDVKEEQLTPYEGRVYLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P153</accession>
    <name>A0A1E3P153_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_84813</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Substrate-binding subunit of tRNA (adenine-N1-)-methyltransferase, which catalyzes the formation of N1-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TRM6/GCD10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038388.1">
        <property type="nucleotide sequence ID" value="XM_019185784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P153"/>
    <dbReference type="STRING" id="683960.A0A1E3P153"/>
    <dbReference type="GeneID" id="30203030"/>
    <dbReference type="OrthoDB" id="10254665at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160107">
        <property type="term" value="F:tRNA (adenine(58)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017423">
        <property type="entry name" value="TRM6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12945">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12945:SF0">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT TRM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04189">
        <property type="entry name" value="Gcd10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038170">
        <property type="entry name" value="tRNA_m1A_mtfrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR038170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="449" mass="50311" checksum="D326455F52E0F5C1" modified="2017-01-18" version="1">MSKESTFIQRPTVIQFGLHCLIRLPSNGLKIAEIKEGSSVNLGKFGVFDLEGIVGYPFGQTFEVLDGRKVRPVRSTTYESENATPVPETLSEKIDYLKRLDIKSSETNRELVDIGNATQGLSMEEIETLKKTSKGANVGEAIIDKLIKSHVNFNMKTKHSQEKYLKRKQQKFLRRFTVEYLGSSQLLQYYLEKDSSKVLDMSEETLGLLLSLGNIQSGGRYLVIDETGGVVVSGLLERMRGSGEVVLVHENEHANLSALEHSNIPQNVQHKMIKTLNLLQFFEPETEKVEWNDLSEEELKEMKSNKKAHYHRRRQNAIDTNAAIENATRGKFDGLIVASTLFPPTLLDRLVEKVGGSRPMVVYSQFKELLVDTQLGFMNDLRILAPTIHESKARPYQTIPGRLHPMMSMRGGGGYLLSALRVFPIEQGVTAVGRGLSKKKQKTQASSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P171</accession>
    <name>A0A1E3P171_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA/pantothenate metabolism flavoprotein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_63580</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPC synthetase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038285.1">
        <property type="nucleotide sequence ID" value="XM_019183574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P171"/>
    <dbReference type="STRING" id="683960.A0A1E3P171"/>
    <dbReference type="GeneID" id="30200820"/>
    <dbReference type="OrthoDB" id="70224at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990143">
        <property type="term" value="C:CoA-synthesizing protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990181">
        <property type="term" value="P:acetyl-CoA biosynthetic process from pantothenate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10300:FF:000002">
        <property type="entry name" value="Phosphopantothenate--cysteine ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12290">
        <property type="entry name" value="CORNICHON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/pantothenate metabolism flavoprotein C-terminal" evidence="2">
        <location>
            <begin position="64"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/pantothenate metabolism flavoprotein C-terminal" evidence="2">
        <location>
            <begin position="188"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="340" mass="38890" checksum="0973AB071CF2A398" modified="2017-01-18" version="1">MPQNIRTSAPDTETAIDRTVPDFPVAESLDEDNYFKTNIPPPYLKDLCAEAEQFVQIHEKTGRKVVLVTSGGTTVPLENNTVRFIDNFSAGTRGATSAEYFLENGYAVIFLHREFSLLPYSRHYSHTTNCFLDYMTEIDGKLEINPKYSEQMHKVWKKYNDARDQNLLLLLPFTTVTQYLFSLKEISKILDKVGTRALFYLAAAVSDFFLPHSKLPEHKIQAQDHNGGKLVVNLDPVPKFLRRLVDSWAPGAMITSFKLETDKSILIKKANQALDRYNHQLVIGNLLQTRKKEVVFVSKNYQNDPVWYRLSDEDVAKGVDIESLIIPAVIKEHQEWIAIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1P7</accession>
    <name>A0A1E3P1P7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Impact N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_94348</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IMPACT family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038470.1">
        <property type="nucleotide sequence ID" value="XM_019186447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1P7"/>
    <dbReference type="STRING" id="683960.A0A1E3P1P7"/>
    <dbReference type="GeneID" id="30203693"/>
    <dbReference type="OrthoDB" id="69641at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140469">
        <property type="term" value="P:GCN2-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.30">
        <property type="entry name" value="Impact, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023582">
        <property type="entry name" value="Impact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001498">
        <property type="entry name" value="Impact_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036956">
        <property type="entry name" value="Impact_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020569">
        <property type="entry name" value="UPF0029_Impact_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301:SF17">
        <property type="entry name" value="IMPACT FAMILY MEMBER YDL177C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301">
        <property type="entry name" value="IMPACT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01205">
        <property type="entry name" value="Impact_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00910">
        <property type="entry name" value="UPF0029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Impact N-terminal" evidence="2">
        <location>
            <begin position="13"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="152" mass="16952" checksum="6413059A355F92E0" modified="2017-01-18" version="1">MINWNESEVLIDRKSKFQGRATIIKPGQDINKLLEDLVANDKSLQKASHKTMHAWRTGTEDVKLGKINPTNQGMSDCGESGAGVRIMTLLERSHLINVLVVVTRWYGGTALGPARFRHITTVAVESLRNGGFLKVEGDDHEKTTKKKSKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1X4</accession>
    <name>A0A1E3P1X4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Hydroxymethylglutaryl-CoA synthase</fullName>
            <shortName evidence="6">HMG-CoA synthase</shortName>
            <ecNumber evidence="6">2.3.3.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">3-hydroxy-3-methylglutaryl coenzyme A synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_63981</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form HMG-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-3-hydroxy-3-methylglutaryl-CoA + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10188"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.10"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the thiolase-like superfamily. HMG-CoA synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.10" evidence="6"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038696.1">
        <property type="nucleotide sequence ID" value="XM_019183648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1X4"/>
    <dbReference type="STRING" id="683960.A0A1E3P1X4"/>
    <dbReference type="GeneID" id="30200894"/>
    <dbReference type="OrthoDB" id="1269963at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00827">
        <property type="entry name" value="init_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000008">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000590">
        <property type="entry name" value="HMG_CoA_synt_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013746">
        <property type="entry name" value="HMG_CoA_synt_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013528">
        <property type="entry name" value="HMG_CoA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010122">
        <property type="entry name" value="HMG_CoA_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01833">
        <property type="entry name" value="HMG-CoA-S_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323:SF2">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08540">
        <property type="entry name" value="HMG_CoA_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01154">
        <property type="entry name" value="HMG_CoA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01226">
        <property type="entry name" value="HMG_COA_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase N-terminal" evidence="7">
        <location>
            <begin position="6"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase C-terminal" evidence="8">
        <location>
            <begin position="178"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="4">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00073381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="450" mass="49258" checksum="1FB3A40B2578236F" modified="2017-01-18" version="1">MTAAARPQNVGIKAIEVYIPSLAINQAELEKFDGVSSGKYTIGLGQTNMSFVNDREDIYSLSLTVLSRLIKNYNIDVNSIGRLEVGTETLLDKSKSVKSVLVQLLGENTDVEGIDTINACYGGTNAVFNAINWVESSAWDGRDAVVVAGDIAIYDKGAARPTGGAGTVALLIGPDAPIVFDNIRGSYFEHVYDFYKPDFTSEYPYVDGHFSLACYVKALDKSYAAYSKKALKAGVEGLTSKTVGATKRFDYNAFHVPTCKLVVKSYARLLYNDFRENPELYPEVDANLAKLSPEESLTDKTVEKTFVALAKELTKERVQPALQIPTNTGNLYTGSLYASLASLLYYVGNDKLQGKRIGLFSYGSGLAASLFSAVVKGDISHITNNLNFDKVLASRNYVSPEEYEAALALREAAHLKKDFKPTGSIDHIASGSYYLTEVNDKFHRKYAIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P250</accession>
    <name>A0A1E3P250_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Luciferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_30536</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the NtaA/SnaA/DszA monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038776.1">
        <property type="nucleotide sequence ID" value="XM_019181741.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P250"/>
    <dbReference type="STRING" id="683960.A0A1E3P250"/>
    <dbReference type="GeneID" id="30198987"/>
    <dbReference type="OrthoDB" id="5561043at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051260">
        <property type="entry name" value="Diverse_substr_monoxygenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016215">
        <property type="entry name" value="NTA_MOA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03860">
        <property type="entry name" value="FMN_nitrolo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011">
        <property type="entry name" value="ALKANESULFONATE MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011:SF16">
        <property type="entry name" value="C2H2 FINGER DOMAIN TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000337">
        <property type="entry name" value="NTA_MOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="6">
        <location>
            <begin position="37"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="493" mass="55453" checksum="B50351CF85F2B802" modified="2017-01-18" version="1">MTSDTKRHKPSKKLIINAFNPGYAAKWRHPRDKSREFTRDVDAWIEYAKLAEKGKLHAIFIADHLVLFDEFEGPANFKHPAKAGQAIPRVDPSAIIPAVAAVTENLGFGITFSTVSEHPYLFHRRLASLDHVTKGRVGWNIVSSYLNSIGRQLLNGAPLPEHDERYVKTEEYLDVVYDLLLSSWRDDALVNDRENATFIDPERIREINHKGKYFDVPGPAITEPSKQRFPVIIQAGTSKKGKELGAGNAEVVYLDDRDKPHLKEKIQSMKKLAKEKFGRSEDSIKFLIASYPIIGKTHEEAEKKLEEISAAAVPLSAEIGFSSISGIDLSKFELDGEVSLGGPNNNLRTTVEGITRQKVPGKSLTKREIIENYKARSGSLFGSYIEVADEIERWVRDYDIDGLNLGVSVYPEGLHDIVDLLVPELQKRGLFHLDYEVPGGTLRENLNGQKGQTFLAKDHPAYGLRWEAGVSLKDFEEQVEKTKKKRQDIRDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2T8</accession>
    <name>A0A1E3P2T8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_53305</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039008.1">
        <property type="nucleotide sequence ID" value="XM_019182710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2T8"/>
    <dbReference type="STRING" id="683960.A0A1E3P2T8"/>
    <dbReference type="GeneID" id="30199956"/>
    <dbReference type="OrthoDB" id="161814at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF115">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0187">Copper transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ59801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="121" mass="13912" checksum="2FBC792C3793D0C1" modified="2017-01-18" version="1">MLFTWDWKNLCVVFRWWHVRSLTSFIVTLVALVVFGIGYEFFKSRAKRYEAKHQGVTNPVKIGSVSRIQKSVYYGLLVGYSYMMMLVFMTYNGWAMIAVVLGAVLGNYLYGGEINDSMACH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P305</accession>
    <name>A0A1E3P305_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_62451</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039073.1">
        <property type="nucleotide sequence ID" value="XM_019183370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P305"/>
    <dbReference type="STRING" id="683960.A0A1E3P305"/>
    <dbReference type="GeneID" id="30200616"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19071">
        <property type="entry name" value="AKR_AKR1-5-like"/>
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    </dbReference>
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        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF13">
        <property type="entry name" value="ALDO_KETO REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="6">
        <location>
            <begin position="20"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="287"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="4">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="296" mass="33613" checksum="FA6CF2D0D6213DD7" modified="2017-01-18" version="1">MTLTKNSQYKLSNNKQIPVIGYGTYQIPPESAQSCTYHALKTGFRHIDCAQRYGNEAQVVQGIVQFLQENPNVSREEIFYTSKIDTPNHGYEITKKSLDLSLSSAKELGYIDLVLIHDPLSDKRGRLSTWMALQEYYKAGKVKAIGVSNYGLPHLKELYESEVLEVKPQVHQISINPWLYREELIKFSQDHGMLVEAYSPLTRGLKFDDEELVEIAKKYNATPVQILVKWSLQMGLLPLTKTLDPIAKGEEILGAFDVKELSKQDLDKLTHKDDYFLPQPQFDPPADTEHLGAKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3S7</accession>
    <name>A0A1E3P3S7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Purine permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_78572</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039155.1">
        <property type="nucleotide sequence ID" value="XM_019184751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3S7"/>
    <dbReference type="STRING" id="683960.A0A1E3P3S7"/>
    <dbReference type="GeneID" id="30201997"/>
    <dbReference type="OrthoDB" id="1641903at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042907">
        <property type="term" value="F:xanthine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006042">
        <property type="entry name" value="Xan_ur_permease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00801">
        <property type="entry name" value="ncs2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42810">
        <property type="entry name" value="PURINE PERMEASE C1399.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42810:SF2">
        <property type="entry name" value="PURINE PERMEASE C1399.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="316"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="403"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="462"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="501"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="546"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="569" mass="61076" checksum="DF95370BB0A7E94E" modified="2017-01-18" version="1">MSAIKSSVNRIIKKVTTKHGLLGDYDYKYLFTPKIPFVKKQSQIQPFFGLNSDMPLLLGFLLGLQHSLSMLAGVVAPPLIIASSAHLSGEASQYLVSVSLIVSGILSLIQITRFHIYKTKYYIGTGLVSVVGTSFATITIVQKAIPMMYANGECPVDADGNFLPCPDGYGAILGTGCVCALLEIFLSFMPPKILQKLFPPMVTGPVVLLIGVHLISSGFSDWLGGSGCSSGKTMCPSNTAPHPLPWGSAQFIGLGFLVFVVIIICEKWGAPIMKSCAVIVGLLVGCIVAAACNYFDRSGIDQSPAVTFIWVHTFKLSVYGPAVLPMLATYIVLMMEAIGDITATCDVSRLEVEGEVYDSRIQGGILADGFNGLLSCLCTMTPMSTFAQNNGVISITKCANRMAGYWCCFFLIVMGIFAKFAAALVSIPKAVLGGMTTFLFTSVAVSGLSIISTVPFTRRNRFILTASLVLGLGSTMVDGWFDKVFTYDGDNRSLKGFFNAIVLVLETGFAVTGFVGVFLNLFIGEEEEEDTAPMYLEGDEVHELDNQSAELEGSSASMSKEQVMNQTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3V8</accession>
    <name>A0A1E3P3V8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_52900</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038774.1">
        <property type="nucleotide sequence ID" value="XM_019182702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3V8"/>
    <dbReference type="STRING" id="683960.A0A1E3P3V8"/>
    <dbReference type="GeneID" id="30199948"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19071">
        <property type="entry name" value="AKR_AKR1-5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000015">
        <property type="entry name" value="Oxidoreductase, aldo/keto reductase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF13">
        <property type="entry name" value="ALDO_KETO REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="6">
        <location>
            <begin position="26"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="5">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="301" mass="34605" checksum="A28739F91E00E8C3" modified="2017-01-18" version="1">MSNVPAYVSRSGYKIPAIALGVYKTPVNEAPKLVHSALNIGYRHIDCAQFYNNEKEVLQGVAEWINENPKTNKRSDVFYTTKIFDQNHGYELTKAAIQQSLDNIKGISDYLDLVLIHSPQSNKEKRLGSYKALQEFAEAGFIHSIGVSNYGIHHLEELYAWEGLKIEPAVNQVELHPWLMRNELVKYCRNKGILLEAYSPLVRGQKFDKDENVMSLSKKYNKSQAQIMIRWSIQQGFVTLPKTSNPGRLASNFDVFNFDNPERLVSNFDVWDFEIKDEDMEILNMPNSYFVTGWDPTTFHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4B3</accession>
    <name>A0A1E3P4B3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_30793</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039345.1">
        <property type="nucleotide sequence ID" value="XM_019181763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4B3"/>
    <dbReference type="STRING" id="683960.A0A1E3P4B3"/>
    <dbReference type="GeneID" id="30199009"/>
    <dbReference type="OrthoDB" id="760868at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013713">
        <property type="entry name" value="XPO2_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF28">
        <property type="entry name" value="IMPORTIN BETA SMX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08506">
        <property type="entry name" value="Cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="7">
        <location>
            <begin position="24"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="680" mass="77234" checksum="AF47E1BA4E7E8495" modified="2017-01-18" version="1">MEAQLISNALQATLDPVPEARKQAEQQLAEFEKQPGFTVYCLDLAVDPSSSNTVKSSAAVYFKNRISKYWKDESDRAIKPEEQETIKTKLIESLVKCYDDSHIRPQLGLAVRNVLLRGAWSLNEPIHQLLNSKSDIAHVYTGLLLLFEATRSQRWAFTDRTLIDTYIEQTFPILEVLASEILNNTDYRSGDMLYLILKIFRYSTISALPKYFYDVNKLSTWISLHLAVVQRDLPKEILDLEPADRSFDKRVKAFKWGFGNLHKFYTRYGLPTSKNTSPEFINFFNASIVPEVLKVYFGIIEKWSGGLWLSDNSLYHLISFLEKCVMTTSWSLIEPHFEIILRHLVYPALCQDNLELFEDDPEEYIRRYFDIYRESSTADVAATDFLFVTSHHRTEKLSVVLNLLNEIFSSFSQDQSEVNALKAEGGLRILSSVSMQLSNEKSPVKDQVDQVIDGFVVPLLTNKHQFLRARACETISIVTCSYKNTSILSRVFEGVYNNFKDDSSLAIQVEAAAALKVLIVEPPVVEQISQDVPTIMQKLLHLSRSYELDMIGEVMESFVEEFSTQLEPFAHDLGSTLCDQFIRTATELLELQSTHSNGGGSDGETEKEYQAVGYINTMTTMTISMGKVDLEEVFAPAIKFVLHNAAISFLGEALELAESLAMARKTISPVLWDLYKEAID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4D3</accession>
    <name>A0A1E3P4D3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">60S ribosomal protein L8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_92657</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039548.1">
        <property type="nucleotide sequence ID" value="XM_019186376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4D3"/>
    <dbReference type="STRING" id="683960.A0A1E3P4D3"/>
    <dbReference type="GeneID" id="30203622"/>
    <dbReference type="OrthoDB" id="29563at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001921">
        <property type="entry name" value="Ribosomal_eL8_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00882">
        <property type="entry name" value="RIBOSOMALL7A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="7">
        <location>
            <begin position="121"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="258" mass="28295" checksum="6CB755E36DB75094" modified="2017-01-18" version="1">MAPKGKKVAPAPLAGSKVTKAKGSKNPLAESKPKKFGIGQNIQPKRNLSRFVKWPEYVRLQRQRKVLSMRLKVPPAIAQFQHTLDRNTAAQAFKLFNKYRPETAAQKKERLTKEAAAIAEGKSKQDASPKPFVVKYGLNHVVSLIENKKAKLVLIANDVDPIELVIFLPALCRKLGVPYAIVKGKARLGTLVHLKTAAVAALTEVKPEDEASLATLVSTVTANFNDKFEENRKHWGGHILGAKSQAKAAKRARASEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4E5</accession>
    <name>A0A1E3P4E5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_12972</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Mitochondrial peptide exporter (TC 3.A.1.212) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038969.1">
        <property type="nucleotide sequence ID" value="XM_019180553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4E5"/>
    <dbReference type="STRING" id="683960.A0A1E3P4E5"/>
    <dbReference type="GeneID" id="30197799"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18573">
        <property type="entry name" value="ABC_6TM_ABCB10_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000218">
        <property type="entry name" value="Multidrug ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF2">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002773">
        <property type="entry name" value="ABC_prm/ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="44"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="367"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="613" mass="66968" checksum="57A32E497E55373B" modified="2017-01-18" version="1" fragment="single">ESKKANQEHEKAKIEADKQKQGYALKDLSRLLSLAKYDWKLLTLALSLLLISCSIGMVLPKAYGLVLDAAKNVIDGELPPIIYGLSLYEFVGIVGALLVLGIATNYGRIILLRILGEKLVARLRANVLKKTISQDAEFFDIHKVGDLISRLSTDAFVVSRSITQNLSDGMKAVITGSVGIGAMFLISPQLGMVVIGFTPMVMIATAIFGNKVRKISRGIQQATGELTKIGEEQLSSVKTVQSFVAEQKELRKYNTGIRNLFGLARKEAKLNATFYAGTAVLGDAAFLVTLGYGALLVARGNISVGDLAAFALYADYANNAMYSLAGFYSELMKGSGAASRLFELIDRNPQINATVGEPVLSKPHGEIEFKNVSFSYPTRPKIQIFNELNFKIPANSNVCVVGPSGRGKSTITSLLLRYYDVTGGEILLDGKDISKYSVKSLRRHLGVVQQEPVLMSGSIRDNITYGIKSEHVSEEEIIEAAKKANCHDFIDKFPDGYDTIIGPRGALLSGGQKQRISIARALIKNPAVLILDEATSALDSKSEYAINQTLQQLMRDEKLTTISIAHRLSTIRKADMVIVLGYDGKVAEIGKFEKLYADRNSALYKLLNEEKHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4E8</accession>
    <name>A0A1E3P4E8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HTH APSES-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_62643</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039281.1">
        <property type="nucleotide sequence ID" value="XM_019183411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4E8"/>
    <dbReference type="STRING" id="683960.A0A1E3P4E8"/>
    <dbReference type="GeneID" id="30200657"/>
    <dbReference type="OrthoDB" id="3981239at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH APSES-type" evidence="3">
        <location>
            <begin position="18"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="423"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="505"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="744"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="796"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="666"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="505"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="554"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="848"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="868"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="898"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="908"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="947"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="963"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="987"/>
            <end position="1006"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1134" mass="129878" checksum="5898F5A844CAC646" modified="2017-01-18" version="1">MSKQTRYTQEPPFETYRPLESDIFENEEYYVYCHKDGKNAFEFVRRKVGGWVNLSHFYSLISEKKHEALDQEIENILPHHKRQQKSTPERFYGIWTTLENARAFAKKHGIYNALAPLLAFDRVALVNYMGYQYFEYFVCPTVDIAIRILRDTSKYIERVNMDQVFIAYKKYKGNANLQLQRSFYTYYKDIFKRGESTLLGGHWTDREAAAKFTRKHGFYQILKPILAFPLNDPSQQDPYPIDIVQANIMQYWKATSCDDSFYGFPETFKGGLAPKRPSKERRNRVFRFTDLTVEKRFSIETTKGDDAFKRNLTLLRRLSDGAVNATMLISICVLLKSNDLNLGADDPDFLIHNELYQRDKLENVPCETVTTHSSKYLNGKWCSAKDARFLFAQFNLVKKDKEKEAKEVDGLSQFLNDERFKSVEDQDEDEVLESNGVKPKQEEDDEFVMIPENDFRKAQNDAKKEAIYYDSKFFENANSSDEEELEAADFNDMEDAQGRVGEDVEEILDDDDEDFVPVKGSKPKRRRFSLANSSDVEGGSGNSKKRKTGGVFISEEEDDDDDDGDDDFKEDVDIEDETNSLFVDSQEGKEPQPDIDERLDELKRKEQTETVILALKALVANFDNNFKKNQKSYRIQKEFDGYFQDLQKFRAISTEIKIRSNLTQTLKKIKNFANNYNRKTEELERNWIDQERNGELPSKDQVIKLSGANPYAKLSANKNNNANHNNFINHQANGGANAFSRVHRTISPSHPQPPPPQPTWNQAPVGPNPHQYAPFQQQRNYNNFNNGVQQQQFPKQPVMPRYNPQPVPSQPHVQRHYPGNVPYGANNRQFGRPVPPPGQPHVQQQQQGPPPPVVPPAQRPSPTIRHAQINSPTFPAAQLQRSGGSSPNPYIVNPPNGPSSLGKKNSPIQPPPPPPPRVGVSPKTAQQPPIQPIARRAPQSSEGYVRSSSTTSGGSSSTGNEKFQNKYRERPTELKDSYVPSAYPNGARRSGATPSTSSSSASPRRVVVVPVSKSPINKNAPRIEYKIDKPLSPPSNYIQMNGEGLDYEDMDIGDYEDMLKSKQDEDEVAQKQNDPLEKYRRIVHFETRTKQDLFKRAKVFFQDDPRVGFSERLHAMMLEGYERRHGIKLNIKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4I5</accession>
    <name>A0A1E3P4I5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_84126</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039397.1">
        <property type="nucleotide sequence ID" value="XM_019185652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4I5"/>
    <dbReference type="GeneID" id="30202898"/>
    <dbReference type="OrthoDB" id="3980068at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.270.60">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="170" mass="19636" checksum="A61DD308938F926C" modified="2017-01-18" version="1">MAQHWKKSSIPYFVIVQDQSEVNQQSFTNARRNSSLLQNNSDSHINASNSSSSGNVLSSSKNYYPEIKYVFQDDPEYYDLENVIEENEEAIVLELDSKGEQVESFKSLTPDLQITDVSLKDRDMTKTISINTTRSELKKFDILKPDKNLNYIKQLVDLYRVRSQQLESLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4Q2</accession>
    <name>A0A1E3P4Q2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptide hydrolase</fullName>
            <ecNumber evidence="6">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_62414</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M28 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="6"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039034.1">
        <property type="nucleotide sequence ID" value="XM_019183359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4Q2"/>
    <dbReference type="STRING" id="683960.A0A1E3P4Q2"/>
    <dbReference type="GeneID" id="30200605"/>
    <dbReference type="OrthoDB" id="10013407at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007039">
        <property type="term" value="P:protein catabolic process in the vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000093">
        <property type="entry name" value="Peptide hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF17">
        <property type="entry name" value="AMINOPEPTIDASE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 6" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009027337" description="Peptide hydrolase" evidence="6">
        <location>
            <begin position="17"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="7">
        <location>
            <begin position="156"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="8">
        <location>
            <begin position="270"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="505" mass="54952" checksum="97CB26544BFBB2F4" modified="2017-01-18" version="1">MKLALSTLLSISAASAYLLPFSIPDAQQIFALKTPEAGDNIKTPYEELPLIETEALQDLITVEALNASAIELYKIANASLGQYGHPKRVNGSPGHWKTIAFIIEELKGLKDYFTFETQEFEALDGKVFNYSLSVNGSDIGTVKPFQLTPPVHYLHGELFAVKNNGCSEDDYEGLDPTKKQVALIERGVCAFGDKSSLAGKLGAKAVLIYDNESPTSIISGTLGEPTNTTVGTLGIQRGLALVLLQIIAAGEKVPVTVNINSYVGLIKTKNVVAETIYGDHENVVALGAHSDSVAAGPGINDDGSGTISLLTVAKELSKFQINNAVRFAWWAAEEEGLLGSNYYASNLTPEENHKIRLFMDYDMMASPNFEYQVYNGSNAVNPVGSEELKNLYIDWYEEHNKNWTLIPFDGRSDYVGFIENDIPGGGIAAGAEGKNSQNGKVLDKCYHLLCDDSTNLNWDAFFTNTKLIAHSVAVYAKDLSGFPARNTIEAKSIPEFKYRGSHLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4T4</accession>
    <name>A0A1E3P4T4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial distribution and morphology protein 32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_42876</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the organization of the mitochondrial membranes and the global structure of the mitochondria. Also required for mitochondrial distribution and mobility as well as for the maintenance of mitochondrial DNA nucleoids structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MDM31/MDM32 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039497.1">
        <property type="nucleotide sequence ID" value="XM_019182573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4T4"/>
    <dbReference type="STRING" id="683960.A0A1E3P4T4"/>
    <dbReference type="GeneID" id="30199819"/>
    <dbReference type="OrthoDB" id="17678at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006873">
        <property type="term" value="P:intracellular monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012571">
        <property type="entry name" value="Mdm31/Mdm32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31068">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31068:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08118">
        <property type="entry name" value="MDM31_MDM32"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="150"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ60290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="554" mass="63681" checksum="A5DEC79422119516" modified="2017-01-18" version="1">MPLINPFQPISRLSLSSRSLFSPLFKVVAARNPWNLHQHRDHSLLHHNLNTIVSNPSKSLIRYRQIHSINPIWRDSKALTNYEAPKLPATINQHNVAVGSTIAGGKVATTITKETLLSQANNPWERFAIRSKWFLIKGYRPFNIDEISAFFSWFILSHIIWVILGTTTFFSLLFYGLNSLFAKELVGKLTGKFISWLNPSFDIKFEDAVVPEWKDGMIDFKKVKITTVDDKGLKLDLKADHIKLTLSFKKYYHIKGMIENVEIHGLTGTIDRRDMNSNESEVDWFTNKNYELGNLKILDSFLKIYPKNGEKPLEFAIYNCELPKVRIQWLLVDFFNASTITGSVNNSLYTIHKRQHKYAYVSDIEDDLNPWKRITRLKLDPIDIDALGLNGTQFNWLVDGKADITADIMQPVIEDDEITSSKYIVVDFKIQFNDLKAKIPTEEPAFSNGEKLISMEDLKPIISYVNNKRTESFETSSPNILPPLNFRVVKKLDDLENVSTLSECKLLDLISTEIYIDWLKHVHEYEIEQRNKRIAMWSKTVASQLLVVGIGAMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4V5</accession>
    <name>A0A1E3P4V5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WKF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_16783</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039600.1">
        <property type="nucleotide sequence ID" value="XM_019180710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4V5"/>
    <dbReference type="STRING" id="683960.A0A1E3P4V5"/>
    <dbReference type="GeneID" id="30197956"/>
    <dbReference type="OrthoDB" id="10261563at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019327">
        <property type="entry name" value="WKF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22306">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22306:SF2">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10180">
        <property type="entry name" value="WKF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WKF" evidence="2">
        <location>
            <begin position="83"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="204" mass="23430" checksum="0B9E089FF0FDFD89" modified="2017-01-18" version="1" fragment="single">MTEHVPAWKRIGLKVKQDLEEDPLAITTHLDSGKVTAKQTKQLNKKKRAAQIGDGDGTKKPPKRQKLPKSERPPPPEKDQLVYLKTYSTDRENWKFSKQKQNWILKHIKVIEDKYEEYLINYLAGLQGGSKDRLIESLKKVQESWNASAAEDEEKTTEEETKESENKEVKNDAKNDLESVDSDYAIRARKIVETITGEKIKLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5A9</accession>
    <name>A0A1E3P5A9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dihydroorotase</fullName>
            <ecNumber evidence="4">3.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_31351</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class II DHOase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="4"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039299.1">
        <property type="nucleotide sequence ID" value="XM_019181824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5A9"/>
    <dbReference type="STRING" id="683960.A0A1E3P5A9"/>
    <dbReference type="GeneID" id="30199070"/>
    <dbReference type="OrthoDB" id="1670005at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00117"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01294">
        <property type="entry name" value="DHOase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000041">
        <property type="entry name" value="Dihydroorotase, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00219">
        <property type="entry name" value="PyrC_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004721">
        <property type="entry name" value="DHOdimr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00856">
        <property type="entry name" value="pyrC_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43137">
        <property type="entry name" value="DIHYDROOROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43137:SF1">
        <property type="entry name" value="DIHYDROOROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001237">
        <property type="entry name" value="DHOdimr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00482">
        <property type="entry name" value="DIHYDROOROTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="360" mass="39372" checksum="70D6CE2481C8AB4E" modified="2017-01-18" version="1">MTSIDLGVTADFHVHLRDGKMMELIAPTVREGGVSIAYIMPNLVPPITTIERVVEYKEKLQALAPKTTFLMTFYLSRELTPELIHEAAKIKAIAGVKCYPAGVTTNSSAGVDPNDFSDFYPIFKALEEENLVLNLHGEKPSSDKDGEEIHVLNAEPMFLPALKKLHNDFPNLKIVLEHCTTKAAVDTINEINQGNGSGPIKVAASITAHHLSLIVDNWAGNPINFCKPVAKLPQDRKALVAAATSGKPWFFFGSDSAPHPLHAKAVHVGVCAGVYTQSLAIPYIAQVFDKEGKLDKLKGFVSDFGKDFYEVSSDDLVNKDHCILLKKEQKVPDVLTQGDLQVAPFKAGESLAWSTEWKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5D2</accession>
    <name>A0A1E3P5D2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_62677</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039319.1">
        <property type="nucleotide sequence ID" value="XM_019183416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5D2"/>
    <dbReference type="STRING" id="683960.A0A1E3P5D2"/>
    <dbReference type="GeneID" id="30200662"/>
    <dbReference type="OrthoDB" id="27962at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058565">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058567">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058568">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058564">
        <property type="entry name" value="TPR_TRAPPC9_Trs120"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013935">
        <property type="entry name" value="Trs120_TRAPPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058563">
        <property type="entry name" value="Trs120_TRAPPC9_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512:SF5">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26254">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26280">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26282">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26283">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26251">
        <property type="entry name" value="TPR_TRAPPC9-Trs120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08626">
        <property type="entry name" value="TRAPPC9-Trs120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trs120/TRAPPC9 N-terminal" evidence="4">
        <location>
            <begin position="4"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 TPR region" evidence="5">
        <location>
            <begin position="336"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 first Ig-like" evidence="6">
        <location>
            <begin position="585"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 third Ig-like" evidence="7">
        <location>
            <begin position="891"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 fourth Ig-like" evidence="8">
        <location>
            <begin position="1051"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="190"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="304"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="304"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="317"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF26251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF26254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF26282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF26283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1169" mass="132591" checksum="0D397A0CA6E4669A" modified="2017-01-18" version="1">MEAHSYTAPARVKALLVPLGNISESAFKKYVGKVRSSFEVRLVDVTPTENSLFNPQGFPNGRVIYDFQLSLNDQESIFLHDFEPFRKTFVVIGITDDPNCSNDDIIKLSKLYKSSITHTAIVFSSKSENESNGGKIFYQSKTNIETILCDITRNFLNDLAAYLSSFQHITLRSPGTIGSNDRIKTMETKKKRISSGSSLSLSLGDSSNPKLSLSSSTERRQSKVKGRQLKILGNLYLLAGKYNNALKEFNDAVPLLRTCSDYLWLGSTLEGIGVCLVMLTFLGVPYQMPSSLNSVLHTTLTIPTPSSTPASTPRNSMAINGNSATPSSDISRFSTPELIHHLTVKVLYYYEISQNDHEDYVPQLVYCESILRFVKFMCVVNIGGGFNNSTIDHIVRGKKIYPKLQNSLFDKMDILKFSNKILTVQLKDMDILSQAKIYSSLASIYGDLNLLRKRSFILRTLFVSLIPELSNNDHSTKTYELSELLDTILSIYGIGRSPETALEDSFASRWVQLHKSVIQLCIIICSKVGDNRRVILFKSLLLTRYSDSLTDNEQSRLLTEIQSICRANDLPFTYFDPFLVRNIEIIKPINTPKKQKQASITNNKEDPFIYNPYAKPISSDGVYLVQDEFVEFQISIQNPFAFQLNINDIQIDQFETFRNHFVIQPKAINTLSVLAKPLKSGTAQISSINIKVFDCQLQSFKIAKTLKSEVVRKLKNDKSFHSKVLEEYEENLTKNRVDGKVNTDELTLNVISAQPNIQILSKPDHLMLLEGGSEKLQLTIKNNSPVPANHLEFSVWDSTIDPLKNVLNNKGLPANEVYEIEFFLFKNPLKIINEIAEIKPYETITLDLEIQGKRGMHQVNLILDYGNKVEDEIYTRRVEVPIRVMVNPSTDLAGCDVIPLVQQVDSKRGDVWEYINSQKDCNITDFALFLVDLRNVWNKTLNVNIDYDGFGVKESIVPLETKRILIPIRRIQIQDDDNLKMVPNLSNRQYILPKITKAEDQFIREAFWYREEILKKLRGAWSVGDVKGEIEFRGIRLSQRMLSVLKVDKVDIKVDVEEAVDKKGLHHVLQTDEFFNVSIEIRNNSSNDIGGILRNIPIASNYGSIDRRLLFNGVLQQPLKNSIKPGETVKILIGAVILEKGEYEWGVTLDEFDTEQSHIARNPLRIRAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5M9</accession>
    <name>A0A1E3P5M9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_20802</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039958.1">
        <property type="nucleotide sequence ID" value="XM_019180886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5M9"/>
    <dbReference type="STRING" id="683960.A0A1E3P5M9"/>
    <dbReference type="GeneID" id="30198132"/>
    <dbReference type="OrthoDB" id="3863715at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051714">
        <property type="entry name" value="Znf_CCHC_NABP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23002">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="4"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="48"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="65" mass="7432" checksum="87510835542ABF89" modified="2017-01-18" version="1" fragment="single">KRTCYKCGNLGHHAEDCSSGERLCYNCKKPGHESSKCPNDRSSESKQCYFCKEIGHIQSDCPKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5Q2</accession>
    <name>A0A1E3P5Q2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Metal resistance protein YCF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_28407</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Cooperates for the ATP-dependent vacuolar transport of bilirubin and glutathione conjugates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039817.1">
        <property type="nucleotide sequence ID" value="XM_019181482.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P5Q2"/>
    <dbReference type="GeneID" id="30198728"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015431">
        <property type="term" value="F:ABC-type glutathione S-conjugate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18595">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18603">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000020">
        <property type="entry name" value="ABC metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000450">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000001">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056227">
        <property type="entry name" value="TMD0_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF443">
        <property type="entry name" value="MULTIDRUG-RESISTANCE LIKE PROTEIN 1, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24357">
        <property type="entry name" value="TMD0_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="434"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="459"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="536"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="572"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="947"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="989"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1077"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1179"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="313"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="635"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="949"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="1268"/>
            <end position="1508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ60610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1513" mass="170621" checksum="898C14FB49765433" modified="2017-01-18" version="1">MSFQFFNQDKLEHLTGATDIYNQEHEGISRIVEESTSKTPLNKTIQAFNYYCNSREGWGPISKYADFTLCFINGVFFTFANLILILFGAAEIYKIQSTKSSVPKFRKGWVFHSRMILVLFQIGLSIATAIFVGAHDYKDVAFIQPVLQAVALIVGLHLHYLDYIYNQITSSNLLMYWLFSTIFSLLKVSHLTIRKDFDHYFIVSLFTAINSVLILLLTWLPSKPTLGYNALADDVSPFDVANIFSILTFSWMTPLMKKGHEQYLTEEDLPRLPHSFESSYIQSYFEKKWDKQLNKTSPSLALALLQAFGPSMLLGGFFKAVQDVLQFSQPQMLKFLIQFVTEYNDGAEQPLVKGFMIVVGMFSISVIQTAFLHQYFLKCFNVGMNLKSSLTATIYKKSLLLSNEERGNKATGDIVNLMSVDTQRLQDLTQFGSILWSGPFQIILCLVSLYQLLGNSMWVGVFIMIIMIPLNSIIMRYLKKLQKIQMKNKDERSRVISEILNNIKSLKLYGWEIPYKEKLNDVRNNKELKNLKKMGVVTAFANFQFNIAPFLVSCSTFAVFVLTQDKPLSTDIVFPALTLFNLLSFPLAVVPMAITAFVEASVAIGRLYNFLTSEELQPDAVNHLPKANKVGEVTVKVNDGTFVWQRKPEYKVALSNVNFTAKKGEIACIVGKVGSGKSALIQSILGDLYRVEGSVDIHGNVAYVAQVPWIMNGTVKDNILFGHKYDPVFYEKCVKACALTVDFAILTDGDSTLVGEKGISLSGGQKARLSLARAVYARADVYLLDDVLAAVDEHVGKHLVDHVLGPNGLLNTKTKILATNKISVLTIADSITLLSNGKITEQGTYDEVTKNSSTALYALIKEFGNMKETAPEFKEENVNVDDLISSEEASDVEITSDANSLRRASFQSLRPMRFDDDAKDTRREHREQGKVKWDIYLEYAKACNPRYVVLFIVFIVISMLLSVFGNIWLKHWSEVNSRLGYNPNVGKYLGIYFAFGVSSALATLFQTVILWVFCSIEGSKVLHSAMTNSVLRAPMQFFETTPIGRIMNRFSNDIYKIDEVLARTFSQFFANSIKVSFTIFVICYSTWQFIFIIIPVLFLYSYYQQYYLRTSRELRRLDSVTRSPIYAHFQETLGGVATIRGFAQEARFLFINQSRIDNNMSAYFPSINANRWLAVRLEFLGSIIIIGAAGLCILTLKFGAITAGMVGLSVSYSLQITQSLNWIVRMTVEVETNIVSVERVKEYSELEPEAPEFLEPKPPAHWPSKGEIIFKDYSARYRKDLGLILKKINLKINPKEKIGIVGRTGAGKSTIVTSIFRLIEAAEGEIIIDGIPIQKIGLQDLRHKLSIIPQDSQVFEGTIRGNIDPTNIYTDEEIWNALELSHLKQHVIKMNEDEDKDLGLDVRINEGGSNLSVGQRQLMCLARALLIPSSILILDEATAAVDVETDKVLQETIRKEFKNRTILTVAHRLNTIMDSDRIIVLDKGEVKEFDSPENLLKNKEGLFYSLVNAHEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5U9</accession>
    <name>A0A1E3P5U9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Glycoside hydrolase family 16 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_29776</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKN1/KRE6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039519.1">
        <property type="nucleotide sequence ID" value="XM_019181650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5U9"/>
    <dbReference type="STRING" id="683960.A0A1E3P5U9"/>
    <dbReference type="GeneID" id="30198896"/>
    <dbReference type="OrthoDB" id="412647at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015926">
        <property type="term" value="F:glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02180">
        <property type="entry name" value="GH16_fungal_KRE6_glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005629">
        <property type="entry name" value="Skn1/Kre6/Sbg1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361">
        <property type="entry name" value="BETA-GLUCAN SYNTHESIS-ASSOCIATED PROTEIN KRE6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361:SF1">
        <property type="entry name" value="BETA-GLUCAN SYNTHESIS-ASSOCIATED PROTEIN KRE6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03935">
        <property type="entry name" value="SKN1_KRE6_Sbg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GH16" evidence="9">
        <location>
            <begin position="37"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ60312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="466" mass="53180" checksum="60366BB337E04F36" modified="2017-01-18" version="1">MLLISLIIIISVLSFIVPFIARSNRFPHHEAPPLVFEHISDYIYPQLSGIRTNMVDEDTPREAYVRKNRDGEDWELVFSDEFNAEGRTFYPGMDQFWEAVDLHYAATRDLEWYDPDAVITSNGTLNLRMDVLQNHGLFYRSGMLQSWNKMCHSEGIVEISAKLPGSSLTAGLWPGLWTLGNLARPGYMATTDGVWPYSYDECDAGITANQSSDDGLSQLPGQKLNKCTCKGEDHPNRGVGRGAPEIDLLEGAHSTKVIFGVASQTLQVAPFDIWYQPDYDFIAIHNLSKSNQNSFRGTPFQEMVSTITTINETWFQYVVDPDDPNIILDDQTYFQKYAVEYLSKVQRKEDAYVRFFLGDEPTVTIEGSAMHPDGNIGWRDMPKEPMSMVLNLGLSTAWSQIDWFHINFPVTFEIDYVRIYQPKLKTQLTCDPGDFPTGDYIERHKNAYMNPNLTTWEQAGYEYPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5W0</accession>
    <name>A0A1E3P5W0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="10">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_92613</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the phage and mitochondrial RNA polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="10"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039529.1">
        <property type="nucleotide sequence ID" value="XM_019186375.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P5W0"/>
    <dbReference type="GeneID" id="30203621"/>
    <dbReference type="OrthoDB" id="276422at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001018">
        <property type="term" value="F:mitochondrial promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006391">
        <property type="term" value="P:transcription initiation at mitochondrial promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1320.10:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000041">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.260:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.280:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1320.10">
        <property type="entry name" value="DNA-directed RNA polymerase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024075">
        <property type="entry name" value="DNA-dir_RNA_pol_helix_hairp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046950">
        <property type="entry name" value="DNA-dir_Rpol_C_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002092">
        <property type="entry name" value="DNA-dir_Rpol_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037159">
        <property type="entry name" value="RNA_POL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029262">
        <property type="entry name" value="RPOL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00940">
        <property type="entry name" value="RNA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14700">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01311">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00900">
        <property type="entry name" value="RNA_POL_PHAGE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00489">
        <property type="entry name" value="RNA_POL_PHAGE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase N-terminal" evidence="11">
        <location>
            <begin position="336"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ60322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1299" mass="148055" checksum="B29F1225869C9CF3" modified="2017-01-18" version="1">MLRAITKNRYASRLNPSLKSVIKRANSTEASLIHRNIIHELRHIPAANDMENRTGAPLADAHQESWSSPYDKVFRQSHSKDVMRLWSLLDVCLDSQQFDRAENILEALYQLSIDNDFSFMDDLNKFLWKRSQDATQRNLDDYIKTWSKNFHVSPNARTYAILISAAMNNGAPYRKYSNQLIGKADEMTKVLGNVDVFGVKNLKAFVSENAISPRLVPQEYRYLLEDVRTDDGEGKQNFDYFEEDSTELPPTLAKANAKELVAVDTFGMKVIRHTLLSLKPHLQTKLFDDLNEKLEGKFNFLTSTSEDTTNFFNIYKTLSPEHQRIFESALDDFNVERQSQLESRSFDAAQERWRHDFEELTQHGTLNLSKNLNARLWSWYEAMKPIVKEEIELCKEVDNVNVKDLSRVAKAQHNERVKYAPYLRLLDPNKTTVITILELLKNNSTGGIHEGMRTARAVVSVAKALELEFRSDQLLNSEKNMFKGYDKKTLARADLKKYAATMTAFKSEKLGNMNIVWPQDVRVKLGSVLVSILMHVAKVEVTGVDPITKNPVKGEAPAFYHSYQYHNGAKIGILKIHKALGAHLSADKLVGAVQPQMLPMLVKPRQWTAWNNGGYYYSQNLLVRSRDSPEQLAYLKASTDRLDLVYEGLNVLGETAWTVNERVYKVMSEIWNTGETFLDIPKDQEEMNVPDAPPKNSDPSVINDWKQKVRRLQNEFATNRSMRCDANYKLEIARAYLGEKFYFPHNVDFRGRAYPLSPNFNHMGNDLSRGLLIFWKGKELGDEGLRWLKIHLSNLFGIDKAPLEERVAFADENLENIKKTAEDPMANKEFWTKADKPWQALATIYDLADAYKLEDPTKHISHQPVHQDGTCNGLQHYAALGGDVEGATQVNLVPADRPQDVYTHVAGLVTKRLEAAAAKGDEMAETLKDKIKRKVVKQTVMTNVYGVTYIGATHQIHKQLQDVFDDTESYKLSTYLAKHVFACMRELFEGAHLIQDWLALCAKMISRSIRIDVDTYEVINQKNKPAFMASVIWTTPLNLPIVQPYRAVVKKQVSTNLQTVFISDPFEMHQVDSRKQMAAFPPNFIHSLDASHMLLSAISCRKEGLEFAAVHDSYWTHASDVKTMGNLLREAFIGLHEVDLVSKLKQEFDQRYHGFLQLVEIPLKSSVGAQIMEQRKKLSKELGRPVTLGDEVYIERKRQNLLNSTEPEEVEEGKAMVTTVSLIEDVDVEKLATVKELNKIENSIGTEDEDLSIVKESAVSSGKQGVSKIRVFAPLRLPEIPPKGQFDVQDLRESKYFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5Z5</accession>
    <name>A0A1E3P5Z5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_78876</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039474.1">
        <property type="nucleotide sequence ID" value="XM_019184803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5Z5"/>
    <dbReference type="GeneID" id="30202049"/>
    <dbReference type="OrthoDB" id="10530078at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="329" mass="36792" checksum="F3777DEC7AF105CF" modified="2017-01-18" version="1">MQHLQASSFPQAATPSNSSPFKSPILIKPWGDEISYGGQWSLPGVSSKFGQTRGPIIDINEISFQLLDELDPSFWSLENYLKAVQTFSSPYYQELSLDQLIKSYAASLCLQKIQKDNHYYEKNTLFESPNSSSELGNCQKQLMESNGKFTSNDKMLQLISTLHKLSEISNQIKIFQGFDLSALYQSSVWPNESSISTLVHFFPVFNDLFPLKNTNEKAEICLVNLKSELESATNDKGTNFLSLGSQGFEGFINTVSNEVNSWYSGNLETPTTPRKRSFDLVDANFNNIEETSSLSPIAAAAATNPVNLSSPLVTRTKSQMRKRRRFSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P612</accession>
    <name>A0A1E3P612_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF3533 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_89968</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039975.1">
        <property type="nucleotide sequence ID" value="XM_019186201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P612"/>
    <dbReference type="STRING" id="683960.A0A1E3P612"/>
    <dbReference type="GeneID" id="30203447"/>
    <dbReference type="OrthoDB" id="2140105at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022703">
        <property type="entry name" value="DUF3533"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053001">
        <property type="entry name" value="MNNG_permease-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34814">
        <property type="entry name" value="NITROSOGUANIDINE RESISTANCE PROTEIN SNG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34814:SF1">
        <property type="entry name" value="NITROSOGUANIDINE RESISTANCE PROTEIN SNG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12051">
        <property type="entry name" value="DUF3533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3533" evidence="2">
        <location>
            <begin position="3"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="355" mass="40606" checksum="028C9610F09D2D26" modified="2017-01-18" version="1">MKQYGTWNIKNQTDFLSLATSHNNTIREEVIRQVHHQKYWGGIYVPQDITSNYYQALKTGDNFTDSIEFIYESGRNPQGVPSYVLNIIQSLEKQFVSIGSNSIINNLISELSNDEVSNLLTSNPQLLSSLWWSYFDNRPVFGSIVLGVVQIGLLYLLVLSFHQFNFAASTHQLIAKKLHTRQYLLYHMVASQSAYLVMSLVYSLMTLAFQVPVNATFGRSGFLVEWAFVFLTMSALGSINENAAIQIFARYRPAIGFWIVFYMVTNVSVIFSPLVVMNNFFRYGYAMPMYQGNELLKVVFLNTYKGHMGRNIGILIAWIVVMNIILPFNLRNVKKYVRNVEAKAKAALEKSKEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P623</accession>
    <name>A0A1E3P623_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HAD family hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_16610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039599.1">
        <property type="nucleotide sequence ID" value="XM_019180700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P623"/>
    <dbReference type="STRING" id="683960.A0A1E3P623"/>
    <dbReference type="GeneID" id="30197946"/>
    <dbReference type="OrthoDB" id="10255128at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050849">
        <property type="entry name" value="HAD-like_hydrolase_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28181:SF1">
        <property type="entry name" value="COLD TOLERANCE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28181">
        <property type="entry name" value="UPF0655 PROTEIN YCR015C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12710">
        <property type="entry name" value="HAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="26727" checksum="2894B4B5A09514DF" modified="2017-01-18" version="1" fragment="single">IVMDWDDTITNKDTIQLVAEAAYITTPKFPRDWSHFAELYYSNYKLYTENFGVRNTLKDEFEFQKGLKQIELSSVNEYVGLQLFKDVTLKTLEDQASKVEIKPNFFQVFKKLYDSKLPVIILSCNWTSVIMSKIFKDHGFEQDENFQIITNEFEHTNDKLTGQVKEDVSIRTGADKVEHVRKLLKELNNSGIQDAVYYIGDSATDILPMLEVDYGIIIGDGSALKTLQKLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P632</accession>
    <name>A0A1E3P632_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein bL27m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">54S ribosomal protein L2, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_67552</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL27 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040106.1">
        <property type="nucleotide sequence ID" value="XM_019184092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P632"/>
    <dbReference type="STRING" id="683960.A0A1E3P632"/>
    <dbReference type="GeneID" id="30201338"/>
    <dbReference type="OrthoDB" id="1867012at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000042">
        <property type="entry name" value="50S ribosomal protein L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001684">
        <property type="entry name" value="Ribosomal_bL27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018261">
        <property type="entry name" value="Ribosomal_bL27_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041244">
        <property type="entry name" value="Ribosomal_bL27m_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00062">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15893:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL27M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15893">
        <property type="entry name" value="RIBOSOMAL PROTEIN L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01016">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18471">
        <property type="entry name" value="Ribosomal_L27_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00063">
        <property type="entry name" value="RIBOSOMALL27"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00831">
        <property type="entry name" value="RIBOSOMAL_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Large ribosomal subunit protein bL27m C-terminal" evidence="10">
        <location>
            <begin position="147"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="382" mass="43889" checksum="C70494585FBDBAEA" modified="2017-01-18" version="1">MGFWSANKNVAFIKSAFGLVSNSFPRASVINGPIIQVRTATKRAAGSRTNMKDSAGRRLGSKKGEGEFVRPGQILMRQRGTKFFPGENVGIGKDHTIFALEPGYVRFYLDPFHPRRRFIGIALKKHSRLPTPHFEPRARRFGYVPITDPSKAKFEEGTLKRKQFLMKPILKKEVKAREEKRIQNTALYGEQLIKVVPELTEEEIKIGSERLLAIRNHVKNGLSNEEAKATTTMIYLQNLKLSLKRNETTQEEWTTAQQKYLNLSSKLEKAVTFDNKFNIIKYRSEDERRSLVAQLESTIKELYSKFDKKNTSEIKKLLFNSDVLTQSELIKFKRTYLKAVLPEDIAVTDTKDKKAAIVKRWNYIREKVDVVARSKGAFVTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P657</accession>
    <name>A0A1E3P657_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="23">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">WICANDRAFT_67486</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 23">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphocholine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38583"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:38584"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(out) + ATP + H2O = a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66036"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:66037"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 22">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="23">Membrane</location>
            <topology evidence="23">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 23">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="23"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040025.1">
        <property type="nucleotide sequence ID" value="XM_019184087.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P657"/>
    <dbReference type="GeneID" id="30201333"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070867">
        <property type="term" value="C:mating projection tip membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990531">
        <property type="term" value="C:phospholipid-translocating ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140351">
        <property type="term" value="F:glycosylceramide flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090554">
        <property type="term" value="F:phosphatidylcholine floppase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140346">
        <property type="term" value="F:phosphatidylserine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090556">
        <property type="term" value="F:phosphatidylserine floppase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099040">
        <property type="term" value="P:ceramide translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000048">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000108">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF180">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE DNF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 21 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10 22 23" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 21 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 23" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="581"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="625"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1217"/>
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            <begin position="1245"/>
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            <begin position="1296"/>
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            <begin position="1335"/>
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            <begin position="1362"/>
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            <begin position="1402"/>
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        <location>
            <begin position="213"/>
            <end position="266"/>
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</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P660</accession>
    <name>A0A1E3P660_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 9">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="7 9">2.1.1.216</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_67511</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43140"/>
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            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.216"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ60843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019184089.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140691">
        <property type="term" value="F:RNA folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160104">
        <property type="term" value="F:tRNA (guanine(26)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160103">
        <property type="term" value="F:tRNA (guanine(26)-N2/guanine(27)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:tRNA N2-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="tRNA (guanine(26)-N(2))-dimethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00308">
        <property type="entry name" value="TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631:SF3">
        <property type="entry name" value="TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1 9" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ60843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="497" mass="55315" checksum="F1B2B08E8F053A43" modified="2017-01-18" version="1">MSEINSSDFNIVKEGKAEILFPKKEDVFYNPVQQFNRDLSTAAIRAWSELYGEKNLNKKRKLNSDEPANTEIKPYINILEALSATGLRAIRYAKEIPNVKQIIANDFSQEAVNSINRNIQYNELKSILKSNKGDANAYMYESKANNRLFHVIDLDPYGTATPFIDAAIQNIASDGLLLVTCTDLAVLAGNGYPEKCYALYGGTNLTGDAVHESALRLVLNMIGTTAAKYKKTIEPLLSLSIDFYVRIFIKVKTSPIEVKNLASKTMISYVCSGCGAHHNQPLGKHTTRENGTGKYSLAQGPPVGPHCPYCGFTNHLTGPMYSGPLHNKEFVSKILDINEKQLDDETYGTRKRIEGMLTLALNEVEEPFYFKPEKIASVLKCQVPPLKIIIAGLGNLGYDASLTHATPSAIKTNANWDVLWAVFKEYIKTQAPNDVNKLNQNGAGYKILTNESIKLEGKEISFVKNELSGKIEKLRKLKIVRYQENPTKNWGPKAKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6D5</accession>
    <name>A0A1E3P6D5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Complex 1 LYR protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_27933</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I LYR family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040031.1">
        <property type="nucleotide sequence ID" value="XM_019181426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6D5"/>
    <dbReference type="STRING" id="683960.A0A1E3P6D5"/>
    <dbReference type="GeneID" id="30198672"/>
    <dbReference type="OrthoDB" id="275715at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990221">
        <property type="term" value="C:L-cysteine desulfurase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd20264">
        <property type="entry name" value="Complex1_LYR_LYRM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045297">
        <property type="entry name" value="Complex1_LYR_LYRM4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051522">
        <property type="entry name" value="ISC_assembly_LYR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13166:SF7">
        <property type="entry name" value="LYR MOTIF-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13166">
        <property type="entry name" value="PROTEIN C6ORF149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="2">
        <location>
            <begin position="11"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="104" mass="12453" checksum="D3114A58ECB4E000" modified="2017-01-18" version="1">MSSITPPAKEQVLQLYRQFIRASSRFNNYNFRHYFIRRSRDAFKESKDVKDPEQVHSLLSKAREELQVLQRQSKISQMYSFDKLVVEPLDRHHRNLNETKEGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6H5</accession>
    <name>A0A1E3P6H5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Temperature shock-inducible protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_61599</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040242.1">
        <property type="nucleotide sequence ID" value="XM_019183210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6H5"/>
    <dbReference type="GeneID" id="30200456"/>
    <dbReference type="OrthoDB" id="4069694at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000992">
        <property type="entry name" value="SRP1_TIP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00660">
        <property type="entry name" value="SRP1_TIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133696" description="Temperature shock-inducible protein 1" evidence="1">
        <location>
            <begin position="18"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="95" mass="10365" checksum="A6F6CF8DB75E5FAA" modified="2017-01-18" version="1">MKLSIITLASLATLATAIDQEEVAAVTALYGDINGHLSDYLNYIQQGNALPDGLLNLYQEAQTYKDDSYTTLLSTVDFNAVEGFVTDILSWDRGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6L2</accession>
    <name>A0A1E3P6L2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_54438</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039694.1">
        <property type="nucleotide sequence ID" value="XM_019182737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6L2"/>
    <dbReference type="STRING" id="683960.A0A1E3P6L2"/>
    <dbReference type="GeneID" id="30199983"/>
    <dbReference type="OrthoDB" id="10267969at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF50">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YOR292C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="290" mass="33960" checksum="49C71ED2AB75F972" modified="2017-01-18" version="1">MSIRLDDSEDNISTHRRGSTLDIESQSVSTESNSRSFQDTYYYKIIKFFSKFKILYVPILNWALLSFFITMLYKLILKYNTMYETNVLLTTITANVLLYGLSDTLAQNISCFLAMVQQKNNQRTREGSIGSIQLGNYTNAFSDQPDELALYTDFDEDIFNFHRFVGFMFWGFVMAFVQICWYWVLNHFYTQEPTFVSVLERVMSDQLVFSPISLFCFFSYSNFVLEGGNKYTLSEKIRKIYFSTLIANYMVWPLVQFINFLVMPKQFQVPFSSSIGVIWNCFLSMRNAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6L7</accession>
    <name>A0A1E3P6L7_WICAA</name>
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        <recommendedName>
            <fullName evidence="1">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">WICANDRAFT_30632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="38"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="264" mass="30231" checksum="C49DC07C21457F5E" modified="2017-01-18" version="1">MSSTDQTYYRDGHDASISQTHSWRNVKNSCAYMLNFIKPTDKILDVGCGPGTITYDLGKYVPKGEIIGIEPTKELIDEALGDETTKKSQPNVSFEVCSVYSLPYPDSCFDIVHAHQVIVHLENPLKALQEMKRVLKPGGYLCCRDGDLQACIFYPEVFIEPLFYYLEKATTEFTCWYGGSRLKELFIGVKFKPDKIIHTTSTWCISSRADRELFSNMYIKRLDSIKYNSSDKYTKSQLKEAWLQWANDDKASWVQIHGEVIAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6P8</accession>
    <name>A0A1E3P6P8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_61531</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ60970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6P8"/>
    <dbReference type="STRING" id="683960.A0A1E3P6P8"/>
    <dbReference type="GeneID" id="30200441"/>
    <dbReference type="OrthoDB" id="2534600at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052783">
        <property type="entry name" value="Metabolic/Drug-Res_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47655">
        <property type="entry name" value="QUINIC ACID UTILIZATION ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47655:SF2">
        <property type="entry name" value="QUINIC ACID UTILIZATION ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="35"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="155"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="201"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="600" mass="67928" checksum="5F09F9F26C3B5A36" modified="2017-01-18" version="1" fragment="single">MCSSQKQGVSSSARRSGSAAGESQQSIKRRRISKACDNCRSKKAKCNGKNPCTSCILNEETCVYSDSNKKRGIPSGYTKELETKNFLLQLLLTKLFTEDDSVLLKLTNLLTDPEVKNEFFSQINDLNTTWHESSNIKPLFTHCLLNDLPSLNLDLKDPNLKPLKTPNKEGGSESSPERLSKRHSNAKEEDPTIGGEELERSNSSNDTKSPTHSRPNIDAALNFNKIALQYKGLSSSNSTFSPTAIQHYVQTLPPTSKSPFRVGSLFNIKSTSKNSLPTEILQFPLNTRKLVDSYFQVVHPWLPMVNRFAIVRYTHKSNVKDPNIDGNFIGLIWAILALGQFKFNGFINEESKILSLNCARNSVIALETTTTSTIETIQSMLLLGLFYYEIGEWDSAWVLTSSASRMAVDTRLMINNNQKLMEHEIKLRERTWSGVYLLNLLLSLRLGRSPVVRKADYPIPKISEDDWEEWDGWTEFQIENYENLTLANKKPLKLESARCLSVHNKLLELASLLNLIVTSYIEYNENLDQTGFYKNDHHRLLNTNPEVFTFGTYTINPNETIAVSAIEDELLEWFKSLPPHCQINDDTSNGEEENGKIPHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P879</accession>
    <name>A0A1E3P879_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sister chromatid cohesion protein PDS5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_83371</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040359.1">
        <property type="nucleotide sequence ID" value="XM_019185532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P879"/>
    <dbReference type="STRING" id="683960.A0A1E3P879"/>
    <dbReference type="GeneID" id="30202778"/>
    <dbReference type="OrthoDB" id="200660at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035808">
        <property type="term" value="C:meiotic recombination initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140588">
        <property type="term" value="P:chromatin looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007130">
        <property type="term" value="P:synaptonemal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19953">
        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039776">
        <property type="entry name" value="Pds5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663">
        <property type="entry name" value="ANDROGEN INDUCED INHIBITOR OF PROLIFERATION AS3 / PDS5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663:SF0">
        <property type="entry name" value="PRECOCIOUS DISSOCIATION OF SISTERS 5, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20168">
        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1170"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1182"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1233"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1247"/>
            <end position="1272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1272" mass="145312" checksum="98F632D76A8815D0" modified="2017-01-18" version="1">MTTNRLPKLKFKKSIVSSPKESIPLKELLDRLDALSTELSKVDQEKFDVNSLNNIKDDLINKKLINHKDAGVQSLVACCISDVLRIYAPDAPYTDSELTNIFKLFIKQFKKLADQGNGYYTQQVYLITRLAEVRSIILITDIDDNTALIESIFELFYDSNNNFHKKLEPVIADVLIEIISEWDQISSNVLKLILNRFLTNTSREGASITSNLSSSSASPFNFTLSICNANPDRLARQLTKFFSEVLFENNEGEDSEVQFKNLRKLHNLSVEIWKHVPEILGSVMGLIDNELNADNEKYRILATETVGKILACQSRLNFVHTHRETWISWLKKTLDISPHVRTRWVEEASKALINRTDVLAEISNGLAKTLIDTDERVRLVSTRAISKIPSSVLANKVNNKSILNGLIQLAREKHPEIRQESINTLACLFDDCYQDIFYNDASEGDEVYKIINQIPNHILNLLYINDKNINYLVDKVLVEKILPFESDELKRVERMLNVFQTLDDKGRASFIAFNRRQQQLSGVLTKLIEFSEAFNGGHDGDADVEKKINKTIDWLSVSLPDRYNPKKSLERFTKINNRRLYYLTKLTISPDSDYETIRNSSKELFTRLQDSKVVNSVEDESSYPINDIFQTFKILIYRASLNFYNRSNISSLLKLSENSKYSKIAQELVDNVSTITPAAFKNQIQDLVKLIKDSVPGVGESKVNTLRALFHIFHKMKDYLDDDDSEFFDKILDFASKGTPLEAKFAVKLISLSKHEDALSVQLFDSIYPFDGDSEYFTSHLAVLSQLFLTQHSIVEEKANEITPFLIKNVLLTNEVVGKESDPVWISDEELEKGNDLNLSAKSLALRIFTNRLRSLSEADEESDNSVSSIAEHVLKLLISLIGNGGEIVSSKSGAYPTPRHYQTRLRLDAGLNLLKLAKIPRYNKLIKPTTLNRLVLLIQDEEENVRSIFIHKLTSLLKTESISLKFLPLVYFIAYEPNDSLKNEIRTWIKFSFSKTSSAIANRAQKSIVFEKSITGLLHSIAHHSEFLEYIEQKDDSEEDEGALKAFTFALEYITFFLDTIATEQNISLLFYFATRIKQYRDALFQENDFKERNDAIYNIYRVSDLAQLSIKELQDHKNWTLTTYPGKVDLSSELFASMKSSEEGQKIVSTSFIPESITKALKAKVRQKISSISTSKRGRPHQEPKENEAPKSKKIKADSVKPKKRKKAAKARDEDDSGNYNKGKDSSPNKPLRKSSRAKRISYAQDEEEEEEEEIEEEESDEEEESDDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8A5</accession>
    <name>A0A1E3P8A5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_86568</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040853.1">
        <property type="nucleotide sequence ID" value="XM_019186052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8A5"/>
    <dbReference type="GeneID" id="30203298"/>
    <dbReference type="OrthoDB" id="3982608at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="387"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="439" mass="50600" checksum="1A7AECDBA147F900" modified="2017-01-18" version="1" fragment="single">MAKNLGQCNKILAPDEAGESAFINITALEETSLLVFHVTDIEKTNIPLYFNDQTSLMNIEDGFYLCTKELFDDGHCSNVGEFVIRYENGGPQEEIINKLLKKGDKVSYNVTDDGVYCIYTHAASDGVQYKITQPYGNLSIDEKQELNVLLFSLLPLDVILLVFWGWKWWRLEKQEQKFTSIQKTLCKLCLFTYFYHENHRLVLDYINRNDMTSQSLLWRVLLVLCANGSIWLMITIFWLYHLWINSPSGVIVKEDKKTGTAFLVLLGLYGVVSEFFAIYPTQDVTPVPLLHLVSVFHYGAKILKVFIWTFLLVNLGETIKKSNDSKVKRKFEISKQLSILIPVFIIFTSIFCQFVTSFDTFARSLKKSQSNESVIVNALENSVHYGFVFGLVQNMAYFIIPLINISLIYIWREDKVNENEDINEIELGNFNDRSDYAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8F4</accession>
    <name>A0A1E3P8F4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_59675</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040797.1">
        <property type="nucleotide sequence ID" value="XM_019182856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8F4"/>
    <dbReference type="STRING" id="683960.A0A1E3P8F4"/>
    <dbReference type="GeneID" id="30200102"/>
    <dbReference type="OrthoDB" id="10249311at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990145">
        <property type="term" value="P:maintenance of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000019">
        <property type="entry name" value="40S ribosomal protein S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000530">
        <property type="entry name" value="Ribosomal_eS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047860">
        <property type="entry name" value="Ribosomal_eS12_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11843">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00972">
        <property type="entry name" value="RIBSOMALS12E"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01189">
        <property type="entry name" value="RIBOSOMAL_S12E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="5">
        <location>
            <begin position="26"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="143" mass="15447" checksum="B240CD0BA01B5DD5" modified="2017-01-18" version="1">MSDTEQVEQVEEVVVEEQAGSITIEDALKSVLRTALIHDGLARGLRESSKALSRGEGQLAVLCESVTEASIVTLVEALCNEPEEKIPLIKVSDAKLLGEWAGLGKIDREGNARKVVGASVVVIKNWGADSEERNILLEHFANQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8M0</accession>
    <name>A0A1E3P8M0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_59800</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040925.1">
        <property type="nucleotide sequence ID" value="XM_019182876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8M0"/>
    <dbReference type="STRING" id="683960.A0A1E3P8M0"/>
    <dbReference type="GeneID" id="30200122"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051945">
        <property type="entry name" value="RRM_MRD1_RNA_proc_ribogen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48039">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN 14B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48039:SF5">
        <property type="entry name" value="RNA-BINDING PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="58"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="151"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="333"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="364"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="385" mass="42484" checksum="B2B9254AD8402CE4" modified="2017-01-18" version="1">MPESESISPSAPEALLSATPSELVGNTAVVSVDGTVDEPLTTATVNEEQYEGAKVNPKRLYISNVSYQTTEDDLKDLLKEYEIISIIVPSQTVRGFKSFHQKSLGIAYIEFTSEEDAELAKTEINGKFVDGRALRVKSFVEYSPGNKVIKRSRSRRGPFGKSKDDVKSVPEKIDEEEATLDTGAAEGNEQQEASDNIEGDEPALCCEEASGESEETKSQSPNVEEREVSKDTVFISRLSPKATDGDLRLFFHDYAPTDVYIFKNRFSKKQHPLRFHQRYVSALVTLTVEDGVPKAIEALSNEKVKGKPVIVRAAYVSKIEDVKKAAAARQKKNEKLEQEANAAAQAAVEKSEADNHRDSINVVQEDDEGFEGFENEKPAVQTVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8S0</accession>
    <name>A0A1E3P8S0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerases I, II, and III subunit RPABC3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_24889</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the eukaryotic RPB8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040975.1">
        <property type="nucleotide sequence ID" value="XM_019181068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8S0"/>
    <dbReference type="STRING" id="683960.A0A1E3P8S0"/>
    <dbReference type="GeneID" id="30198314"/>
    <dbReference type="OrthoDB" id="20018at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000191">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005570">
        <property type="entry name" value="RPABC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03870">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000779">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00658">
        <property type="entry name" value="RPOL8c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="143" mass="16002" checksum="844BC976132EB164" modified="2017-01-18" version="1">MSNTLFDDIFNITEVDAARYNKVSRIVGTSSTSSDIKITLDINSDLFPVNVNDALTITLASSLSLEEENKDTTGSWRAPRAGEKSLADDYDYVMYGTVYKFEESSDDKISVYVSFGGLLLCLQGGYRNLSNLKQENIYLLLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8U5</accession>
    <name>A0A1E3P8U5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_59905</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Lipid-anchor</topology>
            <topology evidence="12">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041036.1">
        <property type="nucleotide sequence ID" value="XM_019182900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8U5"/>
    <dbReference type="STRING" id="683960.A0A1E3P8U5"/>
    <dbReference type="GeneID" id="30200146"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000032">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF4">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 12" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009027341" description="1,3-beta-glucanosyltransferase" evidence="12">
        <location>
            <begin position="21"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ61829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="495" mass="54143" checksum="652DFB068D2ECBAB" modified="2017-01-18" version="1">MKITTTTFIQLLLFICSADALIPITVKGKRFIKPNSDASTQGEVFFIKGIDYQPGGSSGYDSATGVDALSNPDACYRDAYAFQQMNINTLRIYSLNPDTNHDECMTIFNNAGIYLILDVNSGEYGGSLNRADPSGSYNANYLNRVFKFIEAFKNYPNVLGFFSGNEVINDQSNYAEITPNYIRAVQRDMKQYIAKHANRTIPVGYSAADSVTLRRATFEYLQCFIDGEENDVSRSDFFGLNSYEWCSPSTWESSGYGELNSTMSNTTLPLLFSEFGCNKNSPRTFNEISDGLFGGLVNTFSGGLVYEYTEEASHYGVVELNSDDSITYREDFENLKNEYANSSIPTILEDDVEKAHIVTCDADIVTNIYSEFGANFTLPAQPKDIADLIEHGVNATNVGKLLDGIEAKKSNHTIYDDSNNEVKDATVVFEASNEINTQSGITASSRVAPVSTVASSGSSSATASSSHSSKGGAHMNSAAGFSFWSVFVVAVSYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8W1</accession>
    <name>A0A1E3P8W1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_86760</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGG/PIGN/PIGO family. PIGO subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041015.1">
        <property type="nucleotide sequence ID" value="XM_019186068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8W1"/>
    <dbReference type="STRING" id="683960.A0A1E3P8W1"/>
    <dbReference type="GeneID" id="30203314"/>
    <dbReference type="OrthoDB" id="272139at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16023">
        <property type="entry name" value="GPI_EPT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037675">
        <property type="entry name" value="PIG-O_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039524">
        <property type="entry name" value="PIGO/GPI13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23071:SF1">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23071">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="421"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="486"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="514"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="543"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="574"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="709"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="749"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ61808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="770" mass="87519" checksum="3DC997FFA3CC4A17" modified="2017-01-18" version="1" fragment="single">MAILYVIIFFSIAVLQGIAIAFFKNGFLLSRQVLDNFSTLEDPIFVDQSLQLKAFDKAVVVIIDALRFDFTIPDESNDVYYHNNFLTPYKLSLDHPENSVLLKFMADPPTTTLQRLKGLTTGSLPTFVDAGSNFDGDVIEEDNFIKQFYQHNRSTTFVGDDTWQAVFGPFLNVSHPYDSLNVWDLHTVDNGVIEHMIPFLDNQKTEKWDLLVGHLLGVDHCGHRYGPDHFSMKDKQIQMDAFIKDVIERIDDKTLLVVMGDHGMDRTGNHGGDSIDELEASLWLYSKKKSFHAIPDRSLYDISNGGKNYRSVDQIDLVPTLSLLMGLPIPYNNLGKPIEEAFKTRDSFKLANPDDTFYEKVLEYQHDSLERCKDLWARFDLLSISIGILLLAFSLVILIIYSKLIPSVVITQLTEEIVPSIIAMVSIFSVIFVAISLILKPYFLSLTWFTLLGVAIGIIFGFSIPVFDRYSLKWLFLSLLDKFNGLWSLVAITFMVLHAALFASNSFTIWEDRIVAYLLISFGFLATLKSLQAQKKVDRVMGVYHSLFFMITTRLVSMITICREEQGDQCVPTFKMTWMTIAGLFLIAFFMPSIIKAFFNITSSYQSAAPLWNNMLRIILCMNAIYWGFEYLEHNQSEINISFVRNMDLKSLHYVKLTVARIIAGITLVAANIGWSRGPLCVKLDIKNNETSSREACILGYQNVYGSQYFLLIMNFAAAIMLFTKPLGQISIFVLIYQILSLFELVDILNVRANLISPVVLGLLGFLHFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P928</accession>
    <name>A0A1E3P928_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Nitrogen permease regulator 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3 4">Required for meiotic nuclear division protein 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_26784</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Mediates inactivation of the TORC1 complex in response to amino acid starvation. Required for meiotic nuclear division.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Vacuole membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the NPR3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041017.1">
        <property type="nucleotide sequence ID" value="XM_019181299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P928"/>
    <dbReference type="STRING" id="683960.A0A1E3P928"/>
    <dbReference type="GeneID" id="30198545"/>
    <dbReference type="OrthoDB" id="18648at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056603">
        <property type="entry name" value="HTH_NPRL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005365">
        <property type="entry name" value="Npr3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13153">
        <property type="entry name" value="CGTHBA PROTEIN -14 GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13153:SF5">
        <property type="entry name" value="GATOR COMPLEX PROTEIN NPRL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24064">
        <property type="entry name" value="HTH_NPRL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03666">
        <property type="entry name" value="NPR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0469">Meiosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GATOR1 complex protein NPRL3 C-terminal HTH" evidence="6">
        <location>
            <begin position="613"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="49"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="108"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="557"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="126"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="562"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU368069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="679" mass="77890" checksum="65757ADA1D5CB0C8" modified="2017-01-18" version="1">MSLYLPNPCLQGILLAISTHNGPQLVYHYPPRPRSYGFKATPFTVDNDLLSSEDSSSDSSDSDSSIDDSQEPSSKILGKKLSQKLNADNIANYTSGQTLLDLLDEQDQRREAREKRRRHQSRKKGSISSNSLKRSTNNSEVNSLMNHHHHQQQNDKVFGFDVEFLSEFVSPPKQLCNSRFELTVDDMAFLGLPIHVNDEGRWRSHNKKPNPENTEDEEHTDTGSMSMFHIVFVMNPPMVEYNYRIDEMFHYVVSRLSLVLRYEQAKSNYVWKEVKKILKIKDNNENLSIEELYVKLKSESSLANAIAQCFDSISTSDIANLEINDKLISLQIPIKNQFSSLLPKTTPVLPGSYLSSTTKYNEEEMDHNMAYMALLLQDEPEKIIVDLKTEPLSPLANFIRNIHPMMSLNKLSMINQLDLGQVKSFANHLIYWRRARAIIPLQVKSVFIVSPMAPMEYIDEGMKIFKHEFPSLPSLPSFLSLLSSSKPRQYMTIIPSKDHKDIYLDALAWLIRHGYMTQLMTFVWLKISNTIKLAVEEDLEREGISKRNKDKVKFTDISTDNTSKDKDGKSGEKVAGDGESTHSGRITTSNRREIEIEEEEEEDTILLDPERATAVERRWISKCIKGQPSDLVALFHKMLKYFNGKTPLELIIVRENISRHDLKRLLNGISEHIISVKHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P936</accession>
    <name>A0A1E3P936_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_52490</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040594.1">
        <property type="nucleotide sequence ID" value="XM_019182688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P936"/>
    <dbReference type="STRING" id="683960.A0A1E3P936"/>
    <dbReference type="GeneID" id="30199934"/>
    <dbReference type="OrthoDB" id="28208at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF458">
        <property type="entry name" value="SODIUM-COUPLED NEUTRAL AMINO ACID TRANSPORTER 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="142"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="257"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="336"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="381"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="405"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="67"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ61387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="477" mass="52199" checksum="4D76152931E27762" modified="2017-01-18" version="1">MSKIAYSNIPQHNDDDEDIVNSRDNDSLELSELNSLDYEEATIPNATQDTSTLDSESLAEIPAQLNKSNLKMAFMNMANSILGAGAIGTPFAMSNTGIVGGVFALILLTLLVDFTIRLIVINLKLSGKTGYQDTVDHCFGRVGKVIIIAAQALFAFGGSIGFCIIIGDTIPHILRSFFPDYQNNEFLKFLFARNTIIVFVTLTVSFPLSLSKDISKLAKASALALVSMLVIILIVLIKGPELSPEYKGVLNTKNTIVTPRIFQGISVISFALVCHHNTSFIFHSLKKPSLNRFSLLTHISCFVSMLALGFIGFGGFSVFKDKTKGNILNNFPGNDFVVNIARFCFGFNMLTTYPLEIFVLRDVLKDLYYINYDTPQELSKKSHIIFTTALVLITMTISLTTCNLGALFELIGASTASIMAYILPPMCNLKMTGSTKTLKEKLPYLGCIAFGFAVMFISSTQTIINAIHEKDGAHCSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P973</accession>
    <name>A0A1E3P973_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Morphogenetic regulator of filamentous growth protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_60037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041170.1">
        <property type="nucleotide sequence ID" value="XM_019182926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P973"/>
    <dbReference type="STRING" id="683960.A0A1E3P973"/>
    <dbReference type="GeneID" id="30200172"/>
    <dbReference type="OrthoDB" id="774557at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029005">
        <property type="entry name" value="LIM-bd/SEUSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10378">
        <property type="entry name" value="LIM DOMAIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01803">
        <property type="entry name" value="LIM_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="631"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="175"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="257"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="324"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="692"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="717" mass="79544" checksum="E04F62357C57101F" modified="2017-01-18" version="1">MSTKKAQKINPLAKPNGSQTSTPNNNGGNIDDNNNHLPNQQQMNPQQQHHPQINPSAAMGNVNGGMPPGGMMGNNNMPMNAMNFGMNNPNFRAMPNANIHPQQMAALQAQAHYANMQDPNRNNPQYQQYLQQLQQQNQMYMQMQNQQLQQQQQQHPHQNPQASGINAAIAAPQNQMPQQFQGQIPQQQQAIPQQQQQQFPGQQPNQQQQQQQHPQQQQQLQGAHPFQQPQSQHPTPLQQHQVQPGRQPQQPTPQQQPTPQQAATPQQLPQQAQMGAQQGPKLDNKGSRMPSVAQGQMPQQRPVTAGASMINRGPQGVINTGNIPQQGQAPQQQSQQQPQPQQAPGPQQQQNPQAPGSAGSTGSQGPLNQATLNIMNFRKQMSNLATLKFLDFCELIGVRSDSTKNILYWRKIITDYFSEIGVLRYSVKSGVDSRQFEFSVPIIPRFFFSIIQSGVVRMEIQPGLLRTQVLANGTTYLESSRCCFTHYYSDGSYVNIHGNIRGILNQNLKLDWLDFQTHVFIPGVEWPSLEKILSDEAKVKDLFANQNDNSSAKSAESKRLQLLTNFRSNYKVFHSMSNFGLQESVMRVMQVSDVMAHLKSLMLFSITSDTTGPLNSLDAFVSQSLLANKGNKSEQDLKNPNSRPASSKPGGADDKKSPLNMANSPVPRGAGKVPPLDMSQSTLTKRRRKSNLDNGLSPKSTADSPKSGAKANKKTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P997</accession>
    <name>A0A1E3P997_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_27123</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041192.1">
        <property type="nucleotide sequence ID" value="XM_019181339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P997"/>
    <dbReference type="STRING" id="683960.A0A1E3P997"/>
    <dbReference type="GeneID" id="30198585"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000017">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004762">
        <property type="entry name" value="Amino_acid_permease_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00913">
        <property type="entry name" value="2A0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF1">
        <property type="entry name" value="GENERAL AMINO-ACID PERMEASE GAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="106"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="342"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="401"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="425"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="472"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="507"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="11">
        <location>
            <begin position="78"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ61985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="580" mass="63530" checksum="41778A27D68F41C7" modified="2017-01-18" version="1">MSDLKNKEVVNSTDPEVGDSYSVTHNEPLESGSRWQRFKDSFKTMEDEEIDSSNMTDLEKAIAKTAKSPLHKSLKNRHLQMIAIGGAIGTGLFIGSGGALKTGGPAALLIGWGLVGTMIYATVQALGELAVTFPISGAFNVYSTRFIDPSWGFAMGWNYALQWLVVLPLELVAASITIKYWNSHINPVAWVAIFYVLIVAINFFGVKGYGEAEFVFSFIKVVAVIGFIILGIILNCGGGPQGGYIGGKYWHDPGAFANGFKGVCTVFVTAAFSFAGTELVALASSESANPRKALPRATKQVFWRITLFYIISLTLIGLLVPYTDERLGQGSYNAAASPFVIAIRNAGISGLPSVINVVIMIAVLSVGNSSVFGCSRTITSLAAQGFAPSFFTFVDRKGRPIFAVLLSLFVGLLCFVSASDKQGDVFDWLLALSGLSSVFTWGSACFAHIRFRAALKHQGRDPHTELAYTSQCGVIGSWYGFILNVLILIAQFWVAAFPLKSKSTADGFFKSYLAFPVVIAFYIFHKIWKKNWRLYVKVSEMDLDTGRAEHDLELLKVEIAEEKAYIASKPFYYRAYRFWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9H9</accession>
    <name>A0A1E3P9H9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Cysteine dioxygenase</fullName>
            <ecNumber evidence="2 9">1.13.11.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_19555</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-cysteine + O2 = 3-sulfino-L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20441"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:61085"/>
            <dbReference type="EC" id="1.13.11.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="9">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.20" evidence="2 9"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041081.1">
        <property type="nucleotide sequence ID" value="XM_019180829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9H9"/>
    <dbReference type="STRING" id="683960.A0A1E3P9H9"/>
    <dbReference type="GeneID" id="30198075"/>
    <dbReference type="OrthoDB" id="543511at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017172">
        <property type="term" value="F:cysteine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019448">
        <property type="term" value="P:L-cysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10548">
        <property type="entry name" value="cupin_CDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010300">
        <property type="entry name" value="CDO_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918">
        <property type="entry name" value="CYSTEINE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918:SF1">
        <property type="entry name" value="CYSTEINE DIOXYGENASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05995">
        <property type="entry name" value="CDO_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="3 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0883">Thioether bond</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="cross-link" description="3'-(S-cysteinyl)-tyrosine (Cys-Tyr)" evidence="7">
        <location>
            <begin position="89"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ61874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="163" mass="18727" checksum="EE2C7C528F9ED01E" modified="2017-01-18" version="1" fragment="single">NSLKNLIQDLSSSIQETRTENNVKIREVMNLLRYYQSKEEQWTKYALRDKDNNYTRNLVTNIKTHSNLLVLVWEPGKQSNIHGHPNTHCFMKILKGQLQEKLFDPTSSESDRVLKRTTTLPTNSVAYITDELGLHQMKNPTADIAVSLHLYVPKYEAGSGVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9L1</accession>
    <name>A0A1E3P9L1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_76261</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041288.1">
        <property type="nucleotide sequence ID" value="XM_019184352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9L1"/>
    <dbReference type="STRING" id="683960.A0A1E3P9L1"/>
    <dbReference type="GeneID" id="30201598"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF1">
        <property type="entry name" value="PROTEIN YIPF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="8">
        <location>
            <begin position="177"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="318" mass="35407" checksum="95EF142118828D06" modified="2017-01-18" version="1">MSKWKPVNVDPNEADDFDLEPDLIEPDDATTPINKASSKQQYSSSNQPQQNQAQGSSSQANQSSFFNFTTFTKPNIDLSNSLNPILNNEQVKERQFSGGDTLDEPVWQTLQRDLSAIGLRLFSVIWPASLSKLAKVQQHNLIIAARNSGINIGINYDQIEQDIEQDLQNQPIDDSNLKKLDWDLWGPLIFILIFSLILGYLSPKTNSSEVFSSVFALVWLTFAVVAINIQLLGGTISFFSALSTQGYALFPLVLSATISIFIKWSFIRFIIYVLFVAWAIYAATINLKVSGVLPGRVFLAIYPVGLVYATLGWLCVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9N8</accession>
    <name>A0A1E3P9N8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein bL32m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_12946</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040804.1">
        <property type="nucleotide sequence ID" value="XM_019180550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9N8"/>
    <dbReference type="STRING" id="683960.A0A1E3P9N8"/>
    <dbReference type="GeneID" id="30197796"/>
    <dbReference type="OrthoDB" id="2014905at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051991">
        <property type="entry name" value="Mitoribosomal_protein_bL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002677">
        <property type="entry name" value="Ribosomal_bL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21026">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21026:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL32M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01783">
        <property type="entry name" value="Ribosomal_L32p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="137" mass="16323" checksum="542876C238A223C8" modified="2017-01-18" version="1" fragment="single">LRQKLQDYLENKKEQLEDAFSIDNGILRAAPKQKVSPQKRKQKLYGPGSKQLKHLHNLNRCPSCGHYKRAHTLCMHCVSQIQRVWRKDLHATEKPKVVEQELDELDKRILYPGKVESEYSKMLKKRREYVDRRTRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA07</accession>
    <name>A0A1E3PA07_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sas10 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_25894</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041453.1">
        <property type="nucleotide sequence ID" value="XM_019181201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA07"/>
    <dbReference type="STRING" id="683960.A0A1E3PA07"/>
    <dbReference type="GeneID" id="30198447"/>
    <dbReference type="OrthoDB" id="1924577at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007146">
        <property type="entry name" value="Sas10/Utp3/C1D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018972">
        <property type="entry name" value="Sas10_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237:SF8">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09368">
        <property type="entry name" value="Sas10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04000">
        <property type="entry name" value="Sas10_Utp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Sas10 C-terminal" evidence="7">
        <location>
            <begin position="481"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="280"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="400"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="493"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="311"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="342"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="400"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="413"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="426"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="508"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="554" mass="63506" checksum="E12187E45B5BB22A" modified="2017-01-18" version="1">MAKRGQAKSRAQTDEDFGLDEVDSFHAEREKILLNEAGPSRQRDEDEDSDEEVLGVSDASDSEEDDEAEFFGKKDEEDVEDEEDEGAWGSTKGAYYGADDLDDEESAKLIEEEALRQQKRHLEELNMDDFVDEEVEEEWTKSAKKHDFGSTDASSNKTLQTFQSISQLDPKAKRKYISTSHPEFIPLSKELSKLKPTLDGLKERKDENEVLNVKFTALSAYLGAISSYFAVFLALLKDEEPFTMKEHPVMECILSSKEVWRQASELYEDDVSLSDDEKYNTKALDNSENNSSEDASDNDEDVFDSASESMGEAEQDEEESEVEIDISKPRNFKKVKSQAQEDFAEGEIADVDAEEKKTRKRTLRFYTSKIDQQAKKNDERYTGDLDIPYKERLFERQQRLIEEARKRGQHDDNGEDLDAENDYDSQDEKDAREVNADNDSYYNTIKSSKIKEKTQRIEAHNQAVKAAKEGKLAELQENLGEDGKRALNYQILKNKGLTPHRNKDNRNSRVKKRKKYEKAQKKLKSTRAVYSQPSGPYEGEKTGIKKNLTRSTKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAC7</accession>
    <name>A0A1E3PAC7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Genetic interactor of prohibitin 7, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_60425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041572.1">
        <property type="nucleotide sequence ID" value="XM_019182991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAC7"/>
    <dbReference type="GeneID" id="30200237"/>
    <dbReference type="OrthoDB" id="186013at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="264" mass="30833" checksum="8DFBD11DE9A03B9E" modified="2017-01-18" version="1">MIRSRPIISRCIRSYSTTSTSSSNSTAPPPSTRPPPKQREQVTQRQKIELAQQGLKDIFDGFLTTEETFEPIDTKPILANPKSYDTLTDFHRNQVFEEVDKKLRRNWNDVSQDIKRMAYYISYGNYGPRHDLKNNLFRDVVPEDLPFNTPSIAKIGKVGDKVKQLPPVDLEQINAQRKENWNRMTRRLDPFTKFIVYLAAFISLGALYRDKFVEDSKVEVPENSLVLADIQRELESQAKKEEKELNAMKELKKDGKKWYYLWLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAL5</accession>
    <name>A0A1E3PAL5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_82119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041189.1">
        <property type="nucleotide sequence ID" value="XM_019185336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAL5"/>
    <dbReference type="GeneID" id="30202582"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="77" mass="8891" checksum="97D6D646A2AABC09" modified="2017-01-18" version="1">MCTRLYLNLDIEKLLLSAAIFTNDQCQFVVLNHDLQSYVLQKLERHTGLPRHSLIHLYEKDSPIDCTSEYKGTTVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAL7</accession>
    <name>A0A1E3PAL7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial group I intron splicing factor CCM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_66560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041481.1">
        <property type="nucleotide sequence ID" value="XM_019184049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAL7"/>
    <dbReference type="GeneID" id="30201295"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051114">
        <property type="entry name" value="Mito_RNA_Proc_CCM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47934:SF6">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47934">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN PET309, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="783"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="867"/>
            <end position="895"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="895" mass="105465" checksum="70525BC5D29E8942" modified="2017-01-18" version="1">MSSLVKLPSSINLIPNVKKIYTLPLLKSIPRGKLRRNPHDRPRVQSPKFPKRYSKSANILIKKRSIQKLEAMNIDVNKFHHYVAVEKDKMIKEQGFTNTTERQHLNSLQLLNTFKFNTPSYKRFTAFYNTIKNIDDTSVIVTKKFKFLLEEVKREVKEPNADEITPEYLRDFAPQNKEEYQYVLEKQLRRAAGLQPVNARYKCSAELLNVLEYLQATNSILKVSVNEFKEVFEASLFIQDDDLASAVRIIAGRALFALGKIDDLSLDKHYNLITALIENDQLGDAQALIERNPVERPMWYHLRASLAIKCGNMDAAENWIEKTKKLFKVKFVQSDIYVLIIKRYVFGDNFERADYWRKEFEACIEQRGFTRDNYKLPYFVAPPKEKCEYLDKFHPLGGITYLIIIGFYIALKPLNHKVQEMIEFYLKQPNTKPKDLENVILSFRYELSTKIKPHLEKLKNRQAEKYLLSFIEQFQKQHPLVALQEEFKDELLNELAELGGFQTITHIIETTIEQKQKPKDTHFTSLLTALLKRDKVEQAFKILEALESSHLIVQSDPQNIELRQDQLLPSVNAHHYAAFLKYYSNNRDFEGFNSVIERFKEVHLVYNTVFLNQILTTLWKNDKIEEAFKYVDEILLENIHLQDPTPPVHGYLRLYTTVWKLIKVLSFMSLKQNVEVPNIRYVFMKMIHDNVIPTDNLYDTILSVFINEKDYYGAICVLQYLGKVHRTLPHKYTCAKIKKLCKFVKFDNNARNVIPAIDPNKRFEVGHQFQRYQESFEQIKQKTSRKVSLDPFAPQSEGRSPLSGHPRDMERYNDPADQAWKILLYQMMTVVRSQYHFDNEELIRTHDDFGLDYEADRIVKEQEEIQKVKHERSRRKKELKEQQHLKMQHMANLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAP3</accession>
    <name>A0A1E3PAP3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 45</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_82511</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041692.1">
        <property type="nucleotide sequence ID" value="XM_019185390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAP3"/>
    <dbReference type="STRING" id="683960.A0A1E3PAP3"/>
    <dbReference type="GeneID" id="30202636"/>
    <dbReference type="OrthoDB" id="10266265at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="604" mass="69384" checksum="3542035D4F2431B9" modified="2017-01-18" version="1">MNLFEVSNFYLGRIVGGDQQTAPKNENSVLPGSRIKVLLLDEYTTPIISLNSTQSELLQHEIYLINRIDNFNRDKMRHLKCICFLKPTEESINYLLDELRNPKYSSYELYFNNFVTKTQLERLAESDDLEVVTKVEENFQDYLTINNDLFSLELKSPSHRIYGDSLNSWHGAAFNRTVEGLTSLLLSLKVRPVIRYEANSKMAAKLSKELMYGIEKTNASLFDFKMKDSPPLLLILDRKNDPITPLLVPWTFQSMVHELIGIENNTVDLSNTPGITDELKKIVLSAKQDPFYEESMFLNFGDLSDKIKDYVFQYRNKTKTNRTLDTIEDMKRFIEEFPEFKKLSGNVSKHMTLASELDRRINKLRLWEVSELEQNISSHDQHNLDLSEIEKLLLNKPEATGKPPSPPISEDSKVRLVALYALRYETNSNNQTNRLKEILKQQGVPLYKINVIDYLLRLCGVSQRLDDEQTIFDKATSNLITGFKTNHQTNNIYMQHIPRLETILSKAARGKLSEKNYPILSPYQGVYANLATEKAQDIIVFIVGGATYEEARIVADLNQINKNVRIILGGTAIHNTETFINEIEDAGSRWPKTTPGERLHSRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB38</accession>
    <name>A0A1E3PB38_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SMP-LTD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_87980</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041651.1">
        <property type="nucleotide sequence ID" value="XM_019186132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB38"/>
    <dbReference type="STRING" id="683960.A0A1E3PB38"/>
    <dbReference type="GeneID" id="30203378"/>
    <dbReference type="OrthoDB" id="3980760at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21673">
        <property type="entry name" value="SMP_Mdm34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027536">
        <property type="entry name" value="MDM34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058825">
        <property type="entry name" value="MDM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28185">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28185:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26545">
        <property type="entry name" value="Mdm34_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="11">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="279"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="319"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="432" mass="49271" checksum="9856A4F67343800B" modified="2017-01-18" version="1">MSFLFNWLDLSVLDLKSSINSILSATTIPQLPHKVSISSLSFGEKPPKFEVLDIIDLKEDKFKGLFKFNYDGELNVAFNTDYEANAIKLIDFDPFTKPNFVLTDKSTVLPVEFKIKNIKIDAILTVVYNGRVTVVFNDDPFIDLDIETSLDEFLDDDLFEMIKQDTLNLVTEMLKQDLPEMLHSLTLDDASTSNVSALLEQDINSPTLKRSISSLSLSEQPMNFKLQESLFDTLSLQPQGFTDVIQRVSLSKIEYQNLESTQAHHRLEERKKRRVIKMNKKEKKATKVQKDQKSSSPSLEIPRIEEEVLPEEEASYARTNSNSETSSTTLIDTSFGSSTNSLDDYLNSETLVVKELDPYESLIHPMDLLPFYKNDQNNLKISTNLSRPRGPSSNYINLNNDFKDFIIQKKDLMMTSSQVVDDSDDDDVFYDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB50</accession>
    <name>A0A1E3PB50_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ribose-phosphate pyrophosphokinase 1</fullName>
            <ecNumber evidence="5">2.7.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Phosphoribosyl pyrophosphate synthase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">WICANDRAFT_82624</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">5-phosphoribose 1-diphosphate synthase involved in nucleotide, histidine, and tryptophan biosynthesis. Active in heteromultimeric complexes with other 5-phosphoribose 1-diphosphate synthases (PRS2, PRS3, PRS4 and PRS5).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="5"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041846.1">
        <property type="nucleotide sequence ID" value="XM_019185411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB50"/>
    <dbReference type="STRING" id="683960.A0A1E3PB50"/>
    <dbReference type="GeneID" id="30202657"/>
    <dbReference type="OrthoDB" id="413572at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000017">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000043">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000051">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF57">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="19">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="207"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="213"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00077829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ62639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="426" mass="46772" checksum="F1A0E6ECF4B334A9" modified="2017-01-18" version="1">MRKCKIFVGNSHPELGQHICAKLGVEPAPCTLKKFSNGETSVQIGVSVRDEDVYVIQSGSSTVNDHIMELLILVSACRGGSAKKITAVLPQFPYSKQSKMKKHRGAITARMLANLLIMAGADHVISMDLHASQMQGFFSKPVDNLYGAPSLAKWIRENVEDYKDAVVVSKNPGGTKRVTALADSLKINFAMIHTDRRRNADIYSQNRTQAQLKQRKQSMSRRRPAMPKNDEDEDEEENIILSNGLQTARVVQGHVVDDDFVPESSGPESVISNDGSIPNDQTSYALGGTYDAADSDEDDEPASVSQEKLITLVGSVQGRSAIILDDLIDRPGSFIAAAEHCRLNCGAKKVYVVATHGLFIDDCLEELNDSPYIDKIVVTNTYPLSEEKKKTEKLVTIDVSPIFAECIRRDHYGESISVLFDSLASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB62</accession>
    <name>A0A1E3PB62_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Guanosine-diphosphatase</fullName>
            <ecNumber evidence="11">3.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_88363</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">After transfer of sugars to endogenous macromolecular acceptors, the enzyme converts nucleoside diphosphates to nucleoside monophosphates which in turn exit the Golgi lumen in a coupled antiporter reaction, allowing entry of additional nucleotide sugar from the cytosol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GDP + H2O = GMP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22156"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.1.42"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.42" evidence="11"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041838.1">
        <property type="nucleotide sequence ID" value="XM_019186147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB62"/>
    <dbReference type="STRING" id="683960.A0A1E3PB62"/>
    <dbReference type="GeneID" id="30203393"/>
    <dbReference type="OrthoDB" id="6372431at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004382">
        <property type="term" value="F:GDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045134">
        <property type="term" value="F:UDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009134">
        <property type="term" value="P:nucleoside diphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24040">
        <property type="entry name" value="ASKHA_NBD_GDA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.150:FF:000009">
        <property type="entry name" value="Guanosine-diphosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF83">
        <property type="entry name" value="GUANOSINE-DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01238">
        <property type="entry name" value="GDA1_CD39_NTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="58"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="140"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="184"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="361"/>
            <end position="365"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ62631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="635" mass="68465" checksum="10B91690008F14C9" modified="2017-01-18" version="1">MSGGLRITNAVRNSRFIIGALVIFGIVIFLVSSPSNVGEGLSKAKLGGLDFTSNNNNAGNGAAAVAGSDDDKPVYPPSPKGDGAIGNSPQYADDSIDAPKKPKEQAPKADPGTSPQFADDNAKDVKGSKEKAADQSTPKEAPKPKINEKAPIKGEFDAIKDSNKAYEGVPDKEKVALADDDDSNISNEVSSKPKQQNVANCKKDPEFVVMIDAGSTGSRVHIYEFDICYSPPKLNKETFEMLKPGLSSFDTDTSGAAKSLDPLLKIALDTVPEAKRGCTPVAVKATAGLRLLGEEKSNNILKAVRAHLENDYPFAVVDGDGVSIMSGDDEGVYAWITANYLLGNIGSSEKTPTAAVFDLGGGSTQIVFEPDFKNGEKMIDGEHKYEINFGNRQFTLYQFSHLGYGLMKGREKINTLIVEAAIKDGKLTKGDSEKKTLQSPCIPPGLSVKDVKVTLDNGDKYTVDLKGSDTPAGAQCRFLAESILNKDADCKQSPCSFNGVHQPSLVRTFQKTSDLYIFSYFYDRTNPLGMPSSFTLEELSELAKTVCNGETLWTSAFSAIDGSLKELKDEPQWCLDLSFQVALLHTGYEIPLHRELRTAKKIANNELGWCLGASLPLLDKTTGGWKCRVKEVTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB77</accession>
    <name>A0A1E3PB77_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HIT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_25900</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041835.1">
        <property type="nucleotide sequence ID" value="XM_019181202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB77"/>
    <dbReference type="STRING" id="683960.A0A1E3PB77"/>
    <dbReference type="GeneID" id="30198448"/>
    <dbReference type="OrthoDB" id="3979969at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23024">
        <property type="entry name" value="zf-HIT_ZNHIT2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51083">
        <property type="entry name" value="ZF_HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HIT-type" evidence="3">
        <location>
            <begin position="6"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="143" mass="16324" checksum="2A61D8C8633E5484" modified="2017-01-18" version="1">MLLGTCTICTSEQAKYKCPKCDVQYCSIKCFKSPNHKHDETTPDSKPTQTSAPSLEPSNDSKYSYIFNDEKVKYMLSFKSLQIHLQSIYSIMKNESIPFEKKIDQANEKLTSLRSTGSQENELVEEFCQYITHLLNNKSTSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBM4</accession>
    <name>A0A1E3PBM4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fork-head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_18369</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042023.1">
        <property type="nucleotide sequence ID" value="XM_019180782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBM4"/>
    <dbReference type="STRING" id="683960.A0A1E3PBM4"/>
    <dbReference type="GeneID" id="30198028"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000221">
        <property type="term" value="P:negative regulation of pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00059">
        <property type="entry name" value="FH_FOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22701">
        <property type="entry name" value="FHA_FKH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000030">
        <property type="entry name" value="Forkhead box protein K2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881">
        <property type="entry name" value="CHECKPOINT SUPPRESSOR 1-LIKE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881:SF1">
        <property type="entry name" value="FORK HEAD PROTEIN HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FHA" evidence="8">
        <location>
            <begin position="56"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Fork-head" evidence="9">
        <location>
            <begin position="247"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="247"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="162"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="216"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="176"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="216"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="234"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ62816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="40702" checksum="2EEC7999A4DD565B" modified="2017-01-18" version="1" fragment="single">DQNELINAVISVLAIPENKTVVAEKYANDKNQATEVQAYAKIAGNNWTFYVKSLSISIGRNTEPAPNQPPIPKESTIDIDLGPAKVVSRQHASINYNLNSRMWELFVSGRNGAKIDGSRIPCGPDSTPSPLYSGAVVDIGGTQMMFILPDAQPKISRTILDSVNSKLPKPKNKRLSSIGFNSNNSNSTNNGTTATFTTSMSSNSNSNLKGFQMYNNIDSESDNLNNNGGMGSKQDNDLSKDESRDIKPPYSYATMITQAILSNTEGVLSLSEIYDWISSHYAYYRFSKSGWQNSIRHNLSLNKAFEKVPRRANEPGKGMKWQIADSYKDEFMKKFQNGSLSKVRRGSSVSRQLQLHLAMHNDLPASQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBR3</accession>
    <name>A0A1E3PBR3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cell division control protein 42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_60793</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041947.1">
        <property type="nucleotide sequence ID" value="XM_019183058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBR3"/>
    <dbReference type="STRING" id="683960.A0A1E3PBR3"/>
    <dbReference type="GeneID" id="30200304"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005940">
        <property type="term" value="C:septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007118">
        <property type="term" value="P:budding cell apical bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007119">
        <property type="term" value="P:budding cell isotropic bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071763">
        <property type="term" value="P:nuclear membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045921">
        <property type="term" value="P:positive regulation of exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000222">
        <property type="term" value="P:positive regulation of pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022604">
        <property type="term" value="P:regulation of cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007096">
        <property type="term" value="P:regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060178">
        <property type="term" value="P:regulation of exocyst localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032889">
        <property type="term" value="P:regulation of vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031106">
        <property type="term" value="P:septin ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01874">
        <property type="entry name" value="Cdc42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000236">
        <property type="entry name" value="Cell division control protein 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037874">
        <property type="entry name" value="Cdc42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ62740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="193" mass="21378" checksum="427CED08E6F7CC04" modified="2017-01-18" version="1">MQTIKCVVVGDGAVGKTCLLISYTTNKFPADYVPTVFDNYAVTVMIGDEPYTFGLFDTAGQEDYDRLRPLSYPSTDVFLVCFSITSPASFENVREKWFPEIHHHAPGVPAIIVGTQVDLRDDQIIIERLRRQGAAPIKPEEGEKLAKQLGAVKYVECSALTQRGLKNVFDEAIVAALEPESVAPKRKKKCVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBS0</accession>
    <name>A0A1E3PBS0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">4'-phosphopantetheinyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_25781</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042070.1">
        <property type="nucleotide sequence ID" value="XM_019181190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBS0"/>
    <dbReference type="STRING" id="683960.A0A1E3PBS0"/>
    <dbReference type="GeneID" id="30198436"/>
    <dbReference type="OrthoDB" id="15433at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="entry name" value="4'-phosphopantetheinyl transferase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="4'-phosphopantetheinyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="4'-phosphopantetheinyl transferase" evidence="2">
        <location>
            <begin position="6"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="137" mass="15714" checksum="5A56BA17B822E69E" modified="2017-01-18" version="1">MSKVLGIGTDIVKLARFKSIVLRNGLKSRQTETFAMKILHPEQELPLFQECVAKDQLDRTIRLLAGSWAMKEAVFKCLDDKVQQSQKFKQWYKYNADNGRPMVGGLYVQNNPKEEFLLSISHDDDTLIASVLRQLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBV9</accession>
    <name>A0A1E3PBV9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_26127</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 12">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042110.1">
        <property type="nucleotide sequence ID" value="XM_019181230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBV9"/>
    <dbReference type="STRING" id="683960.A0A1E3PBV9"/>
    <dbReference type="GeneID" id="30198476"/>
    <dbReference type="OrthoDB" id="10056816at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055029">
        <property type="term" value="C:nuclear DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10507">
        <property type="entry name" value="Zn-ribbon_RPA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019761">
        <property type="entry name" value="DNA-dir_RNA_pol-M_15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034004">
        <property type="entry name" value="Zn_ribbon_RPA12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF14">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01030">
        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="6 8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133768" description="DNA-directed RNA polymerase subunit" evidence="13">
        <location>
            <begin position="20"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS-type" evidence="14">
        <location>
            <begin position="80"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="10">
        <location>
            <begin position="10"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ62903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="123" mass="13485" checksum="9DE541DEC4CB93EA" modified="2017-01-18" version="1">MSVVGSLIFCLDCGNLLDAATSDKSITCGDCERSYNAKDFANLKVVTTSSDDAFPSALRLKKSTVKTTLKNDELGEGATIKEKCPQCGNDEMQYHTLQLRSADEGATVFYTCAKCDYRFRTNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC47</accession>
    <name>A0A1E3PC47_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_66719</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0507 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041664.1">
        <property type="nucleotide sequence ID" value="XM_019184051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC47"/>
    <dbReference type="STRING" id="683960.A0A1E3PC47"/>
    <dbReference type="GeneID" id="30201297"/>
    <dbReference type="OrthoDB" id="7464126at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097422">
        <property type="term" value="C:tubular endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045022">
        <property type="term" value="P:early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051248">
        <property type="entry name" value="UPF0507/Ank_repeat_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24170">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24170:SF1">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G09870)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="2">
        <location>
            <begin position="284"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1137" mass="132531" checksum="66D7C9655FADAB70" modified="2017-01-18" version="1">MPTHLPLLLNPLLNAVFNNPQPSKSPLKDIYTNLSKGWEDGTVILVPDLNILLNYADKESGIPYRELCYSEEFLKDHIITTNELRNARLFTTFSKKEVLVKKDILFTGKGFRRSLRLKIHSSEYFHNFNDYFEKGRKFMIMHIEAPLYGTPERLNTLDLITPSNNAAERVVLNDISETIQNEENETEVRNSLDSFVQNETLKFEQILRTFPLVSKSIGEKFETLFRKFNVKDSPDADSLIEVFTETISLSSHIFQLLSPDLIDAILDMSPSLNLNECIFNYVELNLCDKIWNRLCEINGDFLIEHYDDLEFLSLNQRISQAITEFKKLELAVTTVFKIKILVNTINLLSNIESVTVDADTLVGLLLMVIVHSKVTNLESHLFYIQNFSFQNIDLGFVGYSLSTLEGVLYYLKDIENIRTLKKYSQNNQRVISYVETKELISLKEIADTYLGNPDSSLKSRTINGESVLMLAVMSNDYDTWKLLIDYEKVNTLEDILSDTTINGASLLNVALNFENYEIVDDLIEILLSSCTVTELIGYLNMKDIHSRTIGHYLFHYKEIIPKVGFLINWLEKDLNGQTPLFSVCRSYDTPKYPEMIRSVFDTVDKWYTQNSSQFDYEDHIDNRGNTLLHILNSQVDVLLKYQRINLNESNRKSYTPLMIYAKYNRAENIEKIIQDERVNILKPDKNNFTCMDQSKNEQVSELLEQQILLKNSPLVSGKQIGVIRIKLENNEWSIVIRGQINGAYYTTTHSFTDFKNLIELIEIEFPNTFLPLEFVSKNFKVNLNMPSFNKLKINKIIEQLNIFLGSLLSNKPFVDHELLWEFLVQKDFNYQSLLNRTQSKFFNFKQDLLNNLPKANDTFEHQKDQLLLQPEEISEMNFFLKFSTKEISKLRSTYDKLLKVVNFMFVKTDELNQSIELFTEESSIGMKELDFLKKVWKSSTKEFSTDEDLCNTVLYLSLSADEIVKKSNILLNEKIYKWWKLYGELIELNNNYKRFKYVEVKKANKHEQVEVEGLVDVNEESFNNFTNDTSFTLNDNGSQAKKQKPSFLTNFIESKRNKYETKLLAGLHDVKKKLYELNKDMKFSHETLAMEMNNFLIFKTEHLKATMKLYVRKQIKDLKFNNVMLQRGLDDLQSLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCD0</accession>
    <name>A0A1E3PCD0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_50852</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat SWD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042147.1">
        <property type="nucleotide sequence ID" value="XM_019182659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCD0"/>
    <dbReference type="STRING" id="683960.A0A1E3PCD0"/>
    <dbReference type="GeneID" id="30199905"/>
    <dbReference type="OrthoDB" id="27537at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042800">
        <property type="term" value="F:histone H3K4 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031126">
        <property type="term" value="P:sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037867">
        <property type="entry name" value="Swd2/WDR82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861">
        <property type="entry name" value="WD40 REPEAT PROTEIN SWD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="107"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="328" mass="36638" checksum="F9FA50A46325A1D4" modified="2017-01-18" version="1">MSVPLTPETYSKFRVSKVFESTDPEACITSLDFDDSGQFLISTLTNESINLYDAFKGTYSKPIYSKKYGCHLAKFTHQQKNCVYASTKEDNTIRYLSLNDNSYIRYFRGHKGLVTSLEVSPLQDVFCSASLDDTIKLWDTRTSSFQASIPSEKPSYVAFDPSGLVIAVASEATGKVELRDAKQFDAMPFQTCQLPKGFRFNKIEFSNDNKYFLLSSTNQKHIILDAFDLFVHTELGETEPIPPRAWPDTGSACFSPDGRFVFAGNGDGKVALWDLNVEPGQKLPPVLPPISKLIGDTKPRMCAFNPKHLELITADTQLVSTNKPFILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCL5</accession>
    <name>A0A1E3PCL5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SMP-30/Gluconolactonase/LRE-like region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_66864</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMP-30/CGR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041834.1">
        <property type="nucleotide sequence ID" value="XM_019184061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCL5"/>
    <dbReference type="STRING" id="683960.A0A1E3PCL5"/>
    <dbReference type="GeneID" id="30201307"/>
    <dbReference type="OrthoDB" id="423498at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004341">
        <property type="term" value="F:gluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013658">
        <property type="entry name" value="SGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005511">
        <property type="entry name" value="SMP-30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907:SF47">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08450">
        <property type="entry name" value="SGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01790">
        <property type="entry name" value="SMP30FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SMP-30/Gluconolactonase/LRE-like region" evidence="4">
        <location>
            <begin position="18"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="324" mass="36521" checksum="5701157D773C99B4" modified="2017-01-18" version="1">MVKETVIEKPFLNYFGRLSEGPTYNPSNNTFLWVDIIAGQLHRVSLANTTSEEYTSDELLKTQESHEAFATPDEYFGVVYLTNKDEIVLIGGRRGIAEYNFNTKKFQYKFKYDEITHGKLRSNDGNIDPNGDIWQGLMGSFEYGPIDEGKLVKIDSKTGEITTKVDHVLISNGINWSKDGKTLYWTSSLEFIIYKFDYDLKTGELSNKTPFINIKDFLPNFESPEPDGFAITETGDIYTAVFSTSLVLHFNSKGELVEQFKFPAKRITAVTFGGVEHDEMFVTSANLHLDDPTKLDAEPDDLGGAIFRVKLPGAKGVLKPQFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU66</accession>
    <name>A0A1E3NU66_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Replication factor A protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_81566</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the replication factor A protein 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035938.1">
        <property type="nucleotide sequence ID" value="XM_019185257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU66"/>
    <dbReference type="STRING" id="683960.A0A1E3NU66"/>
    <dbReference type="GeneID" id="30202503"/>
    <dbReference type="OrthoDB" id="188186at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04479">
        <property type="entry name" value="RPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013970">
        <property type="entry name" value="Rfa2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08661">
        <property type="entry name" value="Rep_fac-A_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ56731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="12609" checksum="1C4C1CB29EF9DBF1" modified="2017-01-18" version="1">MSDHSTPRIDGSMLEQYTNRTVRVIGKVEDIQGSNLKLYTPSDNQKDLTITVSTTPDLNFEKGYWYEIIGRVMDNLSLRAIDIQNFGENINEKAVAGLVKYSQKASVIFYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU81</accession>
    <name>A0A1E3NU81_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_22666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454217">
        <property type="protein sequence ID" value="ODQ56688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU81"/>
    <dbReference type="STRING" id="683960.A0A1E3NU81"/>
    <dbReference type="OrthoDB" id="2278877at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043457">
        <property type="term" value="P:regulation of cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044792">
        <property type="entry name" value="TAR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47188">
        <property type="entry name" value="PROTEIN TAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47188:SF1">
        <property type="entry name" value="PROTEIN TAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ56688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="59" mass="6525" checksum="A6D47F3407449580" modified="2017-01-18" version="1" fragment="single">SFAPIPKFDDRFARQNRYEPPPEFPLASPYSGIVHHLSGPNSYALTQIHPKTSGSVDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU83</accession>
    <name>A0A1E3NU83_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_65901</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454243">
        <property type="protein sequence ID" value="ODQ56633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035840.1">
        <property type="nucleotide sequence ID" value="XM_019184014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU83"/>
    <dbReference type="STRING" id="683960.A0A1E3NU83"/>
    <dbReference type="GeneID" id="30201260"/>
    <dbReference type="OrthoDB" id="3980873at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="127"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="16"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="61"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ56633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="252" mass="28771" checksum="70D09A8F3E035E80" modified="2017-01-18" version="1" fragment="single">MNSYIQNYGDQPLDLSTAPSSSSNVSSYFMNQHHSQSSNNGNNNNNGISNQFWRKPNTANTTSSSTFTPITSANNNNNNTANSSTSTIPPQYSNQQRSFNNDRHFNQIPTNNNNNNFDYPSNNNEKEERLKIDLRIKESQIESLENEIQRLSESMNLIDSKNNNSLLSNNQQIPKNINEIFNKLTNNYTILENELNDTKKRLESLITSITLNPTNSITNNGRYDELEISHKIILKLDLLKQANEEMLKQLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUQ8</accession>
    <name>A0A1E3NUQ8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_98097</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036021.1">
        <property type="nucleotide sequence ID" value="XM_019186631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUQ8"/>
    <dbReference type="STRING" id="683960.A0A1E3NUQ8"/>
    <dbReference type="GeneID" id="30203877"/>
    <dbReference type="OrthoDB" id="10250504at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041178">
        <property type="entry name" value="RPA43_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF5">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17875">
        <property type="entry name" value="RPA43_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RPA43 OB" evidence="7">
        <location>
            <begin position="60"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="197"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ56814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="234" mass="26090" checksum="3AA5C3C8B03BD52B" modified="2017-01-18" version="1">MIMKHFAPANGVVIGYDNLKLSEEHYGKDENDDQVVVAKLNAHSPFAFFWINVELLVWCPSVGDVVEGLIYMQSPSHIGLLINDTFNASIKKNFIPENWTFIPNQADEFEENNNNNEDDQNTGSKKFQSLGQWVDENEIPIDGKIKFTIRRIHTSGRVVSVEGSLIKPGSELEALPVTPGLNKKIVFEDSALDTIVPDEVKEEVPTYDNANSSDEDDEVVAQNSSSEDDDSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV60</accession>
    <name>A0A1E3NV60_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_85976</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036235.1">
        <property type="nucleotide sequence ID" value="XM_019185977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV60"/>
    <dbReference type="STRING" id="683960.A0A1E3NV60"/>
    <dbReference type="GeneID" id="30203223"/>
    <dbReference type="OrthoDB" id="2241241at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000044">
        <property type="entry name" value="Hexose transporter Hxt3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF50">
        <property type="entry name" value="HEXOSE TRANSPORTER HXT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="49"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="415"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="460"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="483"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="52"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="8"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ57028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="536" mass="59512" checksum="C0C46F7600C4BAE3" modified="2017-01-18" version="1">MDYTKPTNEQNELLNNNSESSGLNNKIDDGDSSNDDTIGNQSLWQLKSVIILCVANTFTGFLFGWDTGTIGGITNMTGFKRHFAPQQSDGSYQFSTITQGLIVSLFNVGCAVGSTFISKLGDYKGRRFAIFISLIIYIIGLVVQMSSVSSAKWYQLMVGRIITGLAVGATSVLTPMFISESAPLKIRGAMVCVYQLMNTLGILFGNVTDYGCKTYHPDTNAEWLIPIGLGYVWAALALLGLFFMPETPFFLAFKNDREGAIKSVAKLNKLPPDSPLVHNEVEKFMARSEKMKEETESVKWTEFITGSPMLGFRLAIGMFIMAMQQLTGANYFFYYGTTLFKSVGLKDTYITSIILATVNCAPTFFSSYLVERFGRKQCLMVGSVWMCMCMYIYASVGGFGLYGSNGEANYSAGCAMIVFSCFFIVGFATTWGPVAYVLVSELYPIRVRATSMSIATAMNWIWNFLISLFTPLITKAIDFKFGYVFAGCLTAAIFLVYLFVPETKGLTSQQIDEIYEDGHIFHLKKHKKPEDEENTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVJ4</accession>
    <name>A0A1E3NVJ4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_47577</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036331.1">
        <property type="nucleotide sequence ID" value="XM_019182603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVJ4"/>
    <dbReference type="STRING" id="683960.A0A1E3NVJ4"/>
    <dbReference type="GeneID" id="30199849"/>
    <dbReference type="OrthoDB" id="1517790at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="523" mass="59627" checksum="C611A585BE67320A" modified="2017-01-18" version="1">MEKHNPLLPLWYDLSRTEDNNALNSAPRDRKTRDENAGGTMFHTRGSGSAILARKRVRDQDEGEHKRRTFDHSNNNSDAILTSDDFVENNPFTAIPNRLTKMSSHPSNPSSESVYFFNQKVPSSPPIHLSGQDLQSSPSKEFNDETMVFTSQATMIPNGTESDMNDSDDGDDSDDTIDFNINNLNRVRGYISSDFSQEDLHYKIDDEELNFKARTVIAKCIEDGNPKIRLDHLGLKNLPDDIEDLKNMVCIGPKGIEVPQIEVYASHNKLRVLPPNFFDVENVTVLSLRHNRMKKLTGKISKFKKLTDLSLASNELKVIPYQILKLANLTNFLVRPNPYLWELKDQEDDFAVQVAEDNECQEINTRRYVSRLKWDMAESVSPVPKLSELSLRVISRYRVSLSETKMWKRNVPTYMQKMIAKAIQKGAYEETCSVCEKVTVNPVAKSLEWWDIQSQKLVPLVRKFCCGNCVKHWLDEVDEAVENFQKGVERKESADCAAGEGVDGEGDMRVDEGTSELRFDEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVP7</accession>
    <name>A0A1E3NVP7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_98272</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454224">
        <property type="protein sequence ID" value="ODQ56657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035864.1">
        <property type="nucleotide sequence ID" value="XM_019186646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVP7"/>
    <dbReference type="STRING" id="683960.A0A1E3NVP7"/>
    <dbReference type="GeneID" id="30203892"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044283">
        <property type="term" value="P:small molecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209:SF812">
        <property type="entry name" value="2OG-FE(II) OXYGENASE FAMILY, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G14880)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209">
        <property type="entry name" value="OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="7">
        <location>
            <begin position="192"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ56657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="395" mass="45089" checksum="8EC50BF51D6F7E57" modified="2017-01-18" version="1">MTTSDVTDKFSELKVSSGESPSSIPTLDLNKATNPETKAEFLDELRNALFNFGFFYIKNPPFDVEKFKNIKEESLKFFEIPLEEKKKVAMVNSKHFLGYNGLGDEITAANVDWREQVEFATELPEPDIKNDDELYKNIEGPNLWPSGDFLPEFKPIVTDYIDELSKLSRQFTHWIEDALGLEYGALEAFFKEVQQAKLKIIKYPDLANLNDQVEAVEKEFANTQYKTKLNQGVGEHRDNDFLTFIYQASHHTSLQVKDFNGEWINVPPVGNTLVFAVGLTLEFITQGVCVSTVHRVLTPTPGEGDRLSISFFQTINIESKKTSIEVDQKAKDLVKLRDAKRTKNKIGFQFQVDETKPVGYSVFLNRVKSHPKVGERHYPRVLKYIQEEVAKASDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVX8</accession>
    <name>A0A1E3NVX8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">SSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_102182</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036357.1">
        <property type="nucleotide sequence ID" value="XM_019180346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVX8"/>
    <dbReference type="STRING" id="683960.A0A1E3NVX8"/>
    <dbReference type="GeneID" id="30197592"/>
    <dbReference type="OrthoDB" id="1914839at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032936">
        <property type="term" value="C:SREBP-SCAP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045540">
        <property type="term" value="P:regulation of cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032933">
        <property type="term" value="P:SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030225">
        <property type="entry name" value="SCAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46378">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN CLEAVAGE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46378:SF1">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN CLEAVAGE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="308"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="386"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="416"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="506"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="633"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="13">
        <location>
            <begin position="279"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1094"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1094"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1109"/>
            <end position="1120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ57150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1242" mass="140242" checksum="521ED3ADA1D0266D" modified="2017-01-18" version="1">MFEFLLFLRSLFQVISDYIYNHHISKLRKYYEKLSFNITKNQRAFIIWPVSIAFILSYPAIYSLYTSPDSLENSYITDLQNIGIGIQSTSFQDPESIDSNALLLKQLWIAPSNLKAFQNGYDIDSGALDKGFLVDALKLQTELLDGINQSEDSFAFVHTPFQYWDNDLERLKSDKFPLRVLQKNSAKTNSSGVSLSHNGLFSGIVKINGLIKSAQAIRILIFYKSGKQNNLDAGKIWDSNLKLIKESNTNSKFFLSEVGQDDHNVKTFLLKLTKMNSIDHFILTTSYVLIALYFVISLSNLRSVRSRIGLLFAFIVELTLAVLSSATLTTYFFQGVDFYQIPLQFLPFVVVVVGIENMFRLVTLLSETPGELSISSRMSKALSGSGITSTLIVFCDLVILSLIYPIVVPNTKQFCIFASFALIIDHILHLTYFAAVLSIDVHRLELHDLLNNDSHFKVTEKKRWYYSLFDTKRRTSLVDSLSENEDQITVSSVANSLKNFILKIKLPFSTTVTGTLVMIMFLIGTNIRWTDDSLKLPLVGKPKQDFGLSTSPQEFSTHIQTYTETQYDLVCFLDKKSFSTEIFKLLENVNQQDFSNVFIAVYNPVVALYGEGDKVKTMSEISFNLNTTYKFDLYYILEFLTSLAFILSIALVILRSLAPDSINDTGDELKKQVIEEDHTFQSKVLSGGHFLDIIKITTSRSPFIVSVGLDHKVLVWSPVSQPMPIPSQLPISSNIWPITHVVLSDHGNYISVFSKTGAIQCYSRLTMTWVWKIEIEELKNNQPLEAFFRHKTIPAFLQRKMAADLLQQARSTPSSRRNSMRSIASPKLGPTMAFANNAGVNKDLEELVIVLKSGQVAVVSCEDGSIKKEKLSAAPLVSSGKLVTPRVNDRLVSCTEDGKLLLSTAVNNKWKTRIVRVDEEKFNNPNITLSGSHTLPYIKEEETPEQSAKLNLEFRKSSIAIVPFVGFIVRTHGCCAEIIDVQTGILIKRFKIRQFKKNTLRVFHDQPTHCRFCGSVSISSFTVVYTTENNSVVMHTFNVDHRAKTSICLRVERDPREIRCVGFNSVTEHQHELGNAEGWTTTDKNQIVGIRRKTEEEVKESLNETRTSTLRKRRAQHHQHHSDENAPRLHDLWEGWAMSADGKVDYYDIPDVGNAGGLLVNSIGQINRFGHKSIVVAFGNIMQVLYLGNDDLIYNDEDKGATNEISGLSFVNKRRKNLKRTDMTSTNFSEVDAIPGISELSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWA3</accession>
    <name>A0A1E3NWA3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_23170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036169.1">
        <property type="nucleotide sequence ID" value="XM_019180978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWA3"/>
    <dbReference type="STRING" id="683960.A0A1E3NWA3"/>
    <dbReference type="GeneID" id="30198224"/>
    <dbReference type="OrthoDB" id="1158011at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="1">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ56962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="151" mass="16866" checksum="7BC88B39AB9C6E0A" modified="2017-01-18" version="1" fragment="single">KQEDPSFNVTPSVGGPLNWHFIINLGPSNSALYGQAQYHGVLFFPIEYPFKPPTIKMITPSGIFEPNTDLKQLMGISKENGDWKTPLNVIQLLQMLLDFMLIDAEIESTEPLFAGNIAGYISSTKMEKFSHASDTLAFNISNPIFKKVFPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWP2</accession>
    <name>A0A1E3NWP2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_64904</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036761.1">
        <property type="nucleotide sequence ID" value="XM_019183830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWP2"/>
    <dbReference type="GeneID" id="30201076"/>
    <dbReference type="OrthoDB" id="4036611at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="359" mass="40514" checksum="F9CE51DAE87C0222" modified="2017-01-18" version="1">MSDKETELTEEQIKEQKLEAARKKYEELKNKNKKKKKKTKKDKKEDEKDEGSGKEESVEPVEDGPKVDVNADAVTEDKSKDDINDEVKEDDQEEVKEEVKEEAKEAKEEAKDEPKEEPKEETTQEKAELENSSKGDLNDESNGEPTNKVNKSTKVPSSEATPNEEDTITQELGELKVTEPLTEEKPVTAATNESTTSDPTEDLFPDSGPSFMESLQQSKADEALLKSQKENELLKKELEKLKNENKSLKMLKLEHLDQLETLEAKVSDLESKLAKARVDSSNHNTLGPSAYDQEDTLSLSPTPSFQQNFPTSFSQFKPHNKNESQLNLSEIKARLSKWKGWNIDMTSWRSVGSGPIVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWW3</accession>
    <name>A0A1E3NWW3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">triacylglycerol lipase</fullName>
            <ecNumber evidence="1">3.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_70724</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.1.3" evidence="1"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036845.1">
        <property type="nucleotide sequence ID" value="XM_019184213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWW3"/>
    <dbReference type="GeneID" id="30201459"/>
    <dbReference type="OrthoDB" id="4059120at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051299">
        <property type="entry name" value="AB_hydrolase_lip/est"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46640:SF3">
        <property type="entry name" value="LIPASE LIH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46640">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G06510)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133562" description="triacylglycerol lipase" evidence="3">
        <location>
            <begin position="19"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="4">
        <location>
            <begin position="82"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="335" mass="37465" checksum="50E8C32463FF7515" modified="2017-01-18" version="1">MKSDIIIYLSILFIHVTAFSDSVYDLLVENAHYCTLAYCSSQSTFEPGTPLDQACPLVDFYDRISGFSYLSVDDVDKKVHSVFRGAHTEGDYLTLLGFAQCPYASVIKHGFKYDDFAEFKNDSTRLKNAILEKSGNDTVCEGCYVHCGTYLEFMKFIQEIYDSATPYLDQHYNLIVDGYSLGGIYALLTGVEFKTQGYNPLVLTYGSLKVGNPKFSKWVDQIFANEQALEIVQAGGDLPYPSYSRVFQTADFAPSLPPSIPILLEYQHAGLQFEITKILLPHPKSVVKFGGPSSSSADDEAILNEETIRDDDIDDDSGYPHLHMFIEFSNTCSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX46</accession>
    <name>A0A1E3NX46_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Palmitoyl-protein thioesterase 1</fullName>
            <ecNumber evidence="2">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyl-protein hydrolase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_34909</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the palmitoyl-protein thioesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="2"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036921.1">
        <property type="nucleotide sequence ID" value="XM_019182252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX46"/>
    <dbReference type="STRING" id="683960.A0A1E3NX46"/>
    <dbReference type="GeneID" id="30199498"/>
    <dbReference type="OrthoDB" id="10263094at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000107">
        <property type="entry name" value="Palmitoyl-protein thioesterase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002472">
        <property type="entry name" value="Palm_thioest"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247:SF8">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE/DOLICHYLDIPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02089">
        <property type="entry name" value="Palm_thioest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00414">
        <property type="entry name" value="PPTHIESTRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="298" mass="34204" checksum="1343B2805C47FA79" modified="2017-01-18" version="1">MHVLKLLTESLGFSALPQQESSKELRPVVFWHGMGDTYNSSAMQRVFSTIHSANPDLFIHSIYINEDESKDQQASLIGNVNDEVEIACAQLESIPELQNTQFDFIGFSQGGIFARAAIERCGLDVHTLITFGSPHAGISDLPRCDDSDWFCKRKNAILKRQVWKKNVQNSIIAAQFFRDPLDYDNYLENSIFLADVNNERFEKNMTYVENLKNLEKLVLVMFSQDETIVPKESAWFYDQDKSSGETIQFKDTGFFKQDLIGLKHLYDQDKIEFLTIDDRHMAIGDDFLKKITEKYLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXR9</accession>
    <name>A0A1E3NXR9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_35626</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036689.1">
        <property type="nucleotide sequence ID" value="XM_019182334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXR9"/>
    <dbReference type="STRING" id="683960.A0A1E3NXR9"/>
    <dbReference type="GeneID" id="30199580"/>
    <dbReference type="OrthoDB" id="2017893at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000786">
        <property type="term" value="P:positive regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14518">
        <property type="entry name" value="DSP_fungal_YVH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016278">
        <property type="entry name" value="DUSP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45848:SF4">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45848">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000941">
        <property type="entry name" value="DUSP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="77"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="5">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000941-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="325" mass="37010" checksum="38E518AB97F7E080" modified="2017-01-18" version="1">MDRVLGGLYIGSYLSLTGSEENFFEQNHITHIVSAFKGELSEDFQSKYTIKSIPVDDDDITNIIQYFDEACIFINQALFPDEIDEFSRGVKKPFKTCVLVICQAGVSRSSTILAAYLMKKYNLSPDQAIHAIQRKRSIAQPNDNFKEQLDLYHELDCQLDEKNPIYRQWLLQHSLKIDPTGQSILSKDSTYVDEGNEEDSKALTQLRCKRCRQKLALSTSFVDHIPPSEDSKQSQFIKKAANSRRIISIQAASNTCSHFFVEPLNWMKNELQGKAEMEGKFQCPKCETKVGGYSWKGSRCSCGKWMVPAIHLQRAKVDEIKLGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXU4</accession>
    <name>A0A1E3NXU4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">mRNA export factor MEX67</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_12836</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the export of mRNA from the nucleus to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NXF family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036719.1">
        <property type="nucleotide sequence ID" value="XM_019180547.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXU4"/>
    <dbReference type="STRING" id="683960.A0A1E3NXU4"/>
    <dbReference type="GeneID" id="30197793"/>
    <dbReference type="OrthoDB" id="25872at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042272">
        <property type="term" value="C:nuclear RNA export factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140602">
        <property type="term" value="C:nucleolar peripheral inclusion body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
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</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXX2</accession>
    <name>A0A1E3NXX2_WICAA</name>
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        </submittedName>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <sequence length="347" mass="35385" checksum="34982F55E3BEE8FB" modified="2017-01-18" version="1">MQLQNLLPAALAILPLVNAYSNVAVRYENLLKRSNETYSIAPVSGVSASSALAPSQDSVSTSELTTEIVTFAPTTDANGQPSSVLTTSTITYYSTVTQTQTPVSTAGGESVLTTESAVETTDDQDSTLTSFILTTVTLPGESSAVSTTSDAQNAVASSSSSADSLALDSNNKNLAPNAVASSSASPSTTDSPVSSSATAQGSVSTVTVTVTNDQCVPTTTTVITTKIPVTAQFTVTGSNGELYTVSTSTDIDQTTTQVVYLTQTTTLPSSSANGIVTTVSNADTTYTTTLASTSIAPSSTGVLYGNGTNASNSTGLSPSASQYSISASDYNSNSISKRSFLRRIIGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXY8</accession>
    <name>A0A1E3NXY8_WICAA</name>
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        <name evidence="4" type="ORF">WICANDRAFT_106380</name>
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        <name type="common">Yeast</name>
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        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
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    </organism>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
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                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
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                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
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                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
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            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
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        <text evidence="1">Belongs to the WAPL family.</text>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="WAPL_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22100">
        <property type="entry name" value="WINGS APART-LIKE PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22100:SF13">
        <property type="entry name" value="WINGS APART-LIKE PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07814">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Wings apart-like protein C-terminal" evidence="3">
        <location>
            <begin position="328"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="95"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="250"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="298"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="134"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="155"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="314"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="653" mass="73499" checksum="4014FA435BA38C07" modified="2017-01-18" version="1">MSSRITYGKKGRRLGTGAARLQPAVEFSPKKENNITDRPLTTSSSTQESDNAPLTSPFNSPTKRAPRKEIVPDVEDQGFDFGVFDLEEDDVKPAMPKKVNRTRTKLKKVESKNESTDTIPTSPSASLGSFFVESSAKPTWSPSKSTSASPKKENITLQKSPTLSFTKKINNSKVEPKKVVRKSLKRPLKLQDTEAAGDVWDLLSDIPQERPKKKTMVFKEKDVEMTDLSTTNSNTESQENELISFFNTQQTDTQPDDEPSTPAPEDTQLLRESTASPRISKTLKTYGLQRSFLLEKQNQDGQDQLGTQEEHDTTLTSEQDQDNTEGLKTISNLRAQGENAQFLDELNFIVEGIETPSSITSSLLELAIRLFDNEFLNLLKHHGLPSFHKDIDADKLLQVFVFGFIACKVLEGGNGKIMFDDQLVHIVKTLLSCNNEVEAIKESRATKAIFKEFKDKLGDIYTPAFLGLTFLIQNNLFINAEIFETLIELFMNLNSKNDQDIKMINMILLLFESFLGSTQAVDDKFEKIVPVLLELNIKDQNCRIALLKVLIVLSVKKFEPVFSEVLVDRSIESSIQNSNTSVGLLELGLLINLVEKDACCKRALNKANLKRLYRLQSKLQGESKDYYSLLIGIMLTKFPHHLIYKTSIRKETH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXZ6</accession>
    <name>A0A1E3NXZ6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 15">Alpha-1,3/1,6-mannosyltransferase ALG2</fullName>
            <ecNumber evidence="5 15">2.4.1.132</ecNumber>
            <ecNumber evidence="4 15">2.4.1.257</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_85178</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 15">Mannosylates Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;3)-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29515"/>
            <dbReference type="Rhea" id="RHEA-COMP:19511"/>
            <dbReference type="Rhea" id="RHEA-COMP:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58472"/>
            <dbReference type="ChEBI" id="CHEBI:132510"/>
            <dbReference type="EC" id="2.4.1.132"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:29516"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>an alpha-D-Man-(1-&gt;3)-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29519"/>
            <dbReference type="Rhea" id="RHEA-COMP:19513"/>
            <dbReference type="Rhea" id="RHEA-COMP:19515"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:132510"/>
            <dbReference type="ChEBI" id="CHEBI:132511"/>
            <dbReference type="EC" id="2.4.1.257"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:29520"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the glycosyltransferase group 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.132" evidence="5 15"/>
    <dbReference type="EC" id="2.4.1.257" evidence="4 15"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037245.1">
        <property type="nucleotide sequence ID" value="XM_019185856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXZ6"/>
    <dbReference type="STRING" id="683960.A0A1E3NXZ6"/>
    <dbReference type="GeneID" id="30203102"/>
    <dbReference type="OrthoDB" id="448893at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004378">
        <property type="term" value="F:GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102704">
        <property type="term" value="F:GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033164">
        <property type="term" value="F:initiation-specific glycolipid 1,6-alpha-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03805">
        <property type="entry name" value="GT4_ALG2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027054">
        <property type="entry name" value="ALG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45918">
        <property type="entry name" value="ALPHA-1,3/1,6-MANNOSYLTRANSFERASE ALG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45918:SF1">
        <property type="entry name" value="ALPHA-1,3_1,6-MANNOSYLTRANSFERASE ALG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="451"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="17">
        <location>
            <begin position="12"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="16">
        <location>
            <begin position="212"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ58038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="478" mass="55224" checksum="10498B4C70E0C5B0" modified="2017-01-18" version="1">MRIAFIHPDLGIGGAERLVVDAALGLQNEGHKVQIFTSHCDKSHCFEEVKNETLKTKVYGDFLPTNFLQKFKILFACLRQLFLVIMLVISGEISEYDYFIVDQLSICLPILHLFQEKRSKTLFYCHFPDQLLAKRTGLIRKIYRLPFDLIEQFTMSTADSIVVNSGFTKTVYDKTFTMIKDFNVPDVIYPCVDLSKETITKETQDVFNDIVGEKTEYFLSINRFEKKKNIELAIKSYKKFLEKNSNSQVKLIVAGGYDHKVEENKQYLAHLEKLSADLNLETLTIFNNEYSKYSNLSKNYNIIFLPSISSNLKELLLSQTKLLIYTPSFEHFGIVPLEAMKFGVPVIAVNNGGPIESIISIDENPELGTGWLEPSDPITWSEKLSKSLEIPKSKLIKNGSIQLNSKFSLKVMTESFEKNMLKTLKKPREKYTWETGMMLWKLPVFFILRKYFGMFQFVALVALVGFYKLRPDYFQYYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY24</accession>
    <name>A0A1E3NY24_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATPase synthesis protein 25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_80157</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial mRNA stabilization factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion inner membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
            <orientation evidence="1 7">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ATP25 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037202.1">
        <property type="nucleotide sequence ID" value="XM_019185014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY24"/>
    <dbReference type="STRING" id="683960.A0A1E3NY24"/>
    <dbReference type="GeneID" id="30202260"/>
    <dbReference type="OrthoDB" id="107372at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140053">
        <property type="term" value="P:mitochondrial gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048255">
        <property type="term" value="P:mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040152">
        <property type="entry name" value="Atp25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28087">
        <property type="entry name" value="ATPASE SYNTHESIS PROTEIN 25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28087:SF1">
        <property type="entry name" value="ATPASE SYNTHESIS PROTEIN 25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02410">
        <property type="entry name" value="RsfS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="573" mass="67220" checksum="F60C8A80A65601D3" modified="2017-01-18" version="1">MGGRLAAEAFRQEELIQDDEYSFEDDKVELANPEQFEHQQNKVETEQEEIEEGDTPWYLREDESSKLDTPIFKTEIPEIPKDAPSNIEPILKFAAEELGLDELKLFDIRNRQDIPASAHIDYMILSTGKSERHIQNAVDELSAFLKKEFKITPFVEGLLKANSIQRQKKRMKRKARKGTYIDLEYGVGPNSWVMVDTKTNGVCFHVLTQQRREAVNLEYLWCKPEEREQYKKIGVTKQVDDNVDSIFHGLQRRNYSTSRSEVVGDEMAIQQFQIGDLNSFDGLKLTNPTSFLKHVIDDLNSKSLEEAQQEYSQFDNAKVEFFNNAFPIIPTRQDFELRYEFFSILNKILPDFYPFDKLTTILMEQSSAGEFITKPQFDQYFQDLILVQQPPKGEIITQQEFNELFKWKFSNLTKILKIMDLQSNVEINNDFLKNLLLLSSQCYNEAHINDVPISRSFKILLDLYHNNQPDYTLIYLILSTFGKDQKYSNFQEYWENNLPIYNVQDNHIELDTRPWFILIQVLDELDKGKLNEWFINSQLPRLIENKVIIDTDMKQQIHKIMDSFGVPELKNTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY36</accession>
    <name>A0A1E3NY36_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ras modification protein ERF4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_34562</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Interacts with ERF2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERF4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037318.1">
        <property type="nucleotide sequence ID" value="XM_019182202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY36"/>
    <dbReference type="STRING" id="683960.A0A1E3NY36"/>
    <dbReference type="GeneID" id="30199448"/>
    <dbReference type="OrthoDB" id="5377273at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031211">
        <property type="term" value="C:endoplasmic reticulum palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019383">
        <property type="entry name" value="Golgin_A_7/ERF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051371">
        <property type="entry name" value="Ras_palmitoyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13254">
        <property type="entry name" value="GOLGI AUTOANTIGEN, GOLGIN SUBFAMILY A, 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13254:SF0">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 7_ERF4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10256">
        <property type="entry name" value="Erf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Golgin subfamily A member 7/ERF4" evidence="8">
        <location>
            <begin position="138"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="62"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="302" mass="35042" checksum="D24A09F01AF61AD2" modified="2017-01-18" version="1">MFFSTFFLFKKSNIALSPKHFKESKQTTNEQLQRLQRRLAGSTTINHTETQQSTATATVKYDTTNTPINKGTDATNQNDHNEPQQDKLLFFNYHEYMVNNYTDLRTGFHDIHDQAVVITHFPNYSVPEDSELNKETRIVRIPRIFGVKGIVYPEYSTLLPGNEAAAIQESIGGESNENDDLIKEFIPKGIYEGQLFGTTSISPLSKYFDDDEFTELMTTINSKIRNCYSSYHFWNILEFLLDMLTFWLLSDIFKFQSKRLLDELEQFIVETNKELEPRGVKIISPRRTAYLSIDFQIPKPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY73</accession>
    <name>A0A1E3NY73_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_95024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037147.1">
        <property type="nucleotide sequence ID" value="XM_019186481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY73"/>
    <dbReference type="GeneID" id="30203727"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011252">
        <property type="entry name" value="Fibrogen-bd_dom1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133561" evidence="1">
        <location>
            <begin position="24"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="77" mass="8640" checksum="28FFC2B79CE2D7CE" modified="2017-01-18" version="1" fragment="single">MLRNFSSFAGAFLVCLLARLAFAKQADEIFNSFNSIQLQSGNGYPMDLYRVVIGWSIDPSMEIGTGDKFSLDMPYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYC6</accession>
    <name>A0A1E3NYC6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamate--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Glutamyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_65048</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="3"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036909.1">
        <property type="nucleotide sequence ID" value="XM_019183852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYC6"/>
    <dbReference type="STRING" id="683960.A0A1E3NYC6"/>
    <dbReference type="GeneID" id="30201098"/>
    <dbReference type="OrthoDB" id="10250478at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017102">
        <property type="term" value="C:methionyl glutamyl tRNA synthetase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10306">
        <property type="entry name" value="GST_C_GluRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1160.10:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.800.10:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000037">
        <property type="entry name" value="Glutamine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000004">
        <property type="entry name" value="Glutamyl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000036">
        <property type="entry name" value="Putative glutamyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1160.10">
        <property type="entry name" value="Glutamyl-trna Synthetase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.800.10">
        <property type="entry name" value="Glutamyl-tRNA Synthetase, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02076">
        <property type="entry name" value="Glu_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004526">
        <property type="entry name" value="Glu-tRNA-synth_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020061">
        <property type="entry name" value="Glu_tRNA_lig_a-bdl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041103">
        <property type="entry name" value="GluRS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00463">
        <property type="entry name" value="gltX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF5">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18466">
        <property type="entry name" value="GluRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate--tRNA ligase N-terminal" evidence="17">
        <location>
            <begin position="23"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Glutathione S-transferase C-terminal" evidence="14">
        <location>
            <begin position="91"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="15">
        <location>
            <begin position="207"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib anti-codon binding" evidence="16">
        <location>
            <begin position="515"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA synthetases class I (E and Q) anti-codon binding" evidence="18">
        <location>
            <begin position="616"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF18466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF20974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ57702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="716" mass="81412" checksum="8E722E24F7BDA0DD" modified="2017-01-18" version="1">MSYTLTVAAKAPVVAYPALIAAAFVNSQTETAIKIDFVDEKAVDKAAVKLTKGDETFVDNQALEKLAADFPKVLLEGDASKEWIKFAAEELTIKDFKKLSVSLEKLDAHLNYRSYIVGYKPSLADIAVWGYLRSNGVIGSVIKNTVYINVSRWYTHLESNKTFGGISELFTKSVAELRKQAKVKPDGKKETHKANFDIDLKDAVDGQVVTRFPPEPSGYLHIGHAKAAILNKYFADQYHGKLIIRFDDTNPSKEKVEFEESIIEDLRLLGIKGDKQTYSSDYFDHMYDLAVKLIKEGKAYADDTPLEKMRQNRMDGIASDRRDRTVEENLKIFTEDMKNATEEGLKNCIRAKISVDNPNKALRDPVIYRCNLTPHHRTGSQWKMYPTYDFCVPVVDSLEGVTHALRTIEYKDRDAQYEWFLKALNIRKVHLWEFARVNFVRTLLSKRKLQWFVDKNYVSNWDDPRFPTVRGVRRRGMTVEGLKNFVISQGPSKNIINLDWSIIWAANKKVIDPVAPRHTAVVSPVKVHIVDGPAKPYTEKKPKHKKNPDVGEKDVIYASDILIDQEDADSFEDGEELTLMDWGNVIITKLVKEGEHVKSIEAKLNLDGDFRKTKHKVTWLADTPEKVSVDLVDFDHLITKDKLEDGDDFENFLTPKTEFHTEGLADVNVASMKVGDIIQFERKGYYRLDKQAEGGKPAVFFTIPDGKTVNKYGAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYD7</accession>
    <name>A0A1E3NYD7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">CSC1/OSCA1-like 7TM region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_34574</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037330.1">
        <property type="nucleotide sequence ID" value="XM_019182203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYD7"/>
    <dbReference type="STRING" id="683960.A0A1E3NYD7"/>
    <dbReference type="GeneID" id="30199449"/>
    <dbReference type="OrthoDB" id="1076608at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022257">
        <property type="entry name" value="PHM7_ext"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF139">
        <property type="entry name" value="PHOSPHATE METABOLISM PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12621">
        <property type="entry name" value="PHM7_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="374"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="420"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="465"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="513"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="558"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="583"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="625"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="652"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="11">
        <location>
            <begin position="13"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="12">
        <location>
            <begin position="186"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="9">
        <location>
            <begin position="372"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="10TM putative phosphate transporter extracellular tail" evidence="10">
        <location>
            <begin position="806"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="739"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="870"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="739"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="750"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="782"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="875"/>
            <end position="888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ58123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="904" mass="100971" checksum="4F04D7C902FDD78A" modified="2017-01-18" version="1">MAGGSDASSSTSAFVSALIFNGVIFLILILLFVFLRPKNKRVYQPKTLNLENVKPEERPPEVPSGPFAWVSFLLSRPQAYLLHYAGLDGYLFLRYLSIFAGLAFIGCIILFPILLPVNATNGNGLSGFELLAFSNVKNENRFFAHVFLSWIYFGLIIFVIYKELVYYVSLRHSIQTTPLYDGLLSSRTLILTDIPEDYLSENEIRRVFPIYSRLWFARDYTELSKLVQERTKLSNKYEGTLNSVISKGVKTRQKLIKKGEPLPEEPQGFIKKEPTHKLGKIPFVGEKVNTIDYSIDRIGELNTEIGDVQKNANVATQLHSVFIEFPNQLEAQRAFQAVPYTDLTKGSRIIGVPPDDIIWENLKMSKTSKLIKRILANTFLTLLIIFWAIPVAVVGCISNINFLIEKVHFLSFINNCPKVLLGLITGILPTVALAILMSLVPVIIKAVGKKSGFVTKQQVELYCQAWFFGFEVVQVFLVVTLASSASSTVTAIIDDPGSAMTLLANNLPKASNFYLSFYLLQGLTAPALALLQIGPLIVSKVLKFLQNTPRKKWEKYNVVPGLSYGVLYPAYQLLVVIMLCYSIIAPILLLFATFTLSLMYIAYIYNLVYVKGQESDMRGRNYPKALLQTFVGLYLSEICLLGLFIMSKSWGPVALEGIMIFVTVACHVLLKRKFQPLFDIVPVSGIYEARGDSGALYPKDQGSKEIKQVGQNYISDNEASGVSNIPNVAGDSSGKVGNFNNDLAGDNQTYESSSENKNIYNNKNITNDSNSSRNETVANDNTRTDTTKENVEQQAKQKPLVVLKRFFDPRSGYSFSLLRSQLPNIFNLSPNYSTEYLDNSYVDPAVTDQEPHIWIPKDPAGISQYQIAKTGGKVDVSDEHTEYNEKGSYEVTGPPPSYEESVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZK2</accession>
    <name>A0A1E3NZK2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA pseudouridine(55) synthase</fullName>
            <ecNumber evidence="3">5.4.99.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_85233</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pseudouridine synthase TruB family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="3"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037319.1">
        <property type="nucleotide sequence ID" value="XM_019185863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZK2"/>
    <dbReference type="STRING" id="683960.A0A1E3NZK2"/>
    <dbReference type="GeneID" id="30203109"/>
    <dbReference type="OrthoDB" id="9995526at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01080">
        <property type="entry name" value="TruB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014780">
        <property type="entry name" value="tRNA_psdUridine_synth_TruB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767:SF2">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE TRUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767">
        <property type="entry name" value="TRNA-PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase II N-terminal" evidence="7">
        <location>
            <begin position="65"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="345"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="345"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="366"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="377" mass="42281" checksum="6E0B5C05E777EE6C" modified="2017-01-18" version="1">MNGILAISKPQGITSSKFIAQLQHIFTDSPVFAKQLADIRGSKMQAIQETSGKKASRRQMRKHTKVKMGHGGTLDPLATGVLVVGVGNGTKKLQNYLSGTVKTYEAEALFGASTTSGDVEGEVLTKNASSHITKQDLEKIPEKFIGHLKQTPPIFAALKMDGKPLYEYARQNLPLPRPIEPREVQIYDLKIEDDSLTKDHDYEFIKSTEVEGDDGEITIEEALSKNPTLNDNKLYFSKEYAEKQGNAEESFEIEKPKPSDGSEKVENYKAPLLHFTASVSSGTYIRSLISDIGKAVQSSAYMVKLIRLQQAEWELEKNVFKLEDFTERDPEVWGPVLKQVFDKGEAEKEYKEKKAKQEEEEEEDGETSKKRTIDQVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0A4</accession>
    <name>A0A1E3P0A4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_100715</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037901.1">
        <property type="nucleotide sequence ID" value="XM_019180304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0A4"/>
    <dbReference type="STRING" id="683960.A0A1E3P0A4"/>
    <dbReference type="GeneID" id="30197550"/>
    <dbReference type="OrthoDB" id="16816at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050602">
        <property type="entry name" value="Malonyl-ACP_OMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13090">
        <property type="entry name" value="ARGININE-HYDROXYLASE NDUFAF5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13090:SF1">
        <property type="entry name" value="ARGININE-HYDROXYLASE NDUFAF5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="33934" checksum="2AE2DF8A79CD9F71" modified="2017-01-18" version="1">MAQLLSNTSKVEYLRNEVAKRTIERLSFIKRDFPKLMDLGSHSGNFAKVLNEGDDEDTRLVRNKIGEILMVDSSKEMLHRWDDDPFNTELKVRKLVVDEEELNHKELEANKFDGIVSNLSLHWINDLPTTLKKINNCLKPDGLFLGSMICEDSIFELRTSLQLAELERKNGMSMSRISPMIKVDDMTNLLKMAKFNMVTIDVEEIIVDYPDILAMMEDLQLMGENNANKQGFENLDRDVLISAQAIYSKFYGEADADVSHLPLTFRVMFMIGWKESGNQPQPLQRGSGELNLKDVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0A7</accession>
    <name>A0A1E3P0A7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="5">PPIase</shortName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_84519</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family. PPIL3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037992.1">
        <property type="nucleotide sequence ID" value="XM_019185722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0A7"/>
    <dbReference type="STRING" id="683960.A0A1E3P0A7"/>
    <dbReference type="GeneID" id="30202968"/>
    <dbReference type="OrthoDB" id="3977984at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01928">
        <property type="entry name" value="Cyclophilin_PPIL3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000012">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF2">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="6">
        <location>
            <begin position="6"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="169" mass="18578" checksum="9648C418D0A4A848" modified="2017-01-18" version="1">MSVTLHTDVGDIKLEIFCEECPKAAENFLAHCAQGTYDGTKILRSIKSFIVQFGDPTNTGKGGESIWGSYFEDEFNESLRHSQRGVLSMANTGPNTNGSQFFITYGKHNTLDGKYTVFGKTLIGAESSSDSTLSKLETLEVDKKKRPKSPVHIQKVTIHANPIAEKFGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0D5</accession>
    <name>A0A1E3P0D5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Putative peptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_64475</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037544.1">
        <property type="nucleotide sequence ID" value="XM_019183744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0D5"/>
    <dbReference type="STRING" id="683960.A0A1E3P0D5"/>
    <dbReference type="GeneID" id="30200990"/>
    <dbReference type="OrthoDB" id="4689212at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11307">
        <property type="entry name" value="M35_Asp_f2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029482">
        <property type="entry name" value="HRXXH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039124">
        <property type="entry name" value="PRA1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39399">
        <property type="entry name" value="PROTEIN ZPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39399:SF1">
        <property type="entry name" value="PROTEIN ZPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13933">
        <property type="entry name" value="HRXXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133598" description="Putative peptidase domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Putative peptidase" evidence="3">
        <location>
            <begin position="12"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="260"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="287" mass="31830" checksum="6B901BD660244CA6" modified="2017-01-18" version="1">MKFSTVLLSLLPALTLAQSQSSDSEQTSTIWVEASETSAYDWSKDWYKDFPVHTSCNRTKFNQLQAAFQDVKALAGQARDHTLRYGNESKFYQKYFGNATTGEVVGWFTNIVDQDKTGVLFRCDDIDQNCHQAGWAGHWRGENGSDETVICDLSFSSRLFLSQMCSQGYQVTKDKTTVYWAGDLLHRIWHTTKIGQDVVGHYADTYDECVELAESSPEEAVRNSASLRYYALEVYAYDVAVPGEGCSGDGESHDHDHASSSESATASTETSGGSECHTHANGETHCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0F9</accession>
    <name>A0A1E3P0F9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_33270</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019182050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0F9"/>
    <dbReference type="STRING" id="683960.A0A1E3P0F9"/>
    <dbReference type="GeneID" id="30199296"/>
    <dbReference type="OrthoDB" id="1935484at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000106">
        <property type="entry name" value="MFS transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF65">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="390"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="445"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="477"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="512"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="570" mass="65758" checksum="AE81D6ED3A25C610" modified="2017-01-18" version="1">MSFLTKWFNPTIIKNQERAESFNDAQSNFSTRNTLSKNETHHHTHEYSHDEISWDSESQLEHVFKDPAVAKYYKKVYESTNYECKEYFDPDYTWTAKEEKKLVWKLDWYVTFWAYIMFTALDFDRSNIQQAVSDNMLDDLGLTTEHYNIGNTINLVCFLASELPSQLVSKKLGADVWIPTQLVLWSAVSISQAAITGKHGFYATRALIGAFQGGFICDACLWMSYFYTSKELPFRLSLFYIANPLTSVFSALLAFALLRIKTSLLPHGWQWLFIIEGVFTFIIGLVSYFKMPPSAVQTKTWFRKKGWFSDHEEKIVVNRVLRDDPSKGDMNNRQPVSPKELLKTLCDFDLLPIYFVRILADIGTSPVSTYMTLTLKKMGFSTFNTNLLTIPYNILSIITMLLLGYFSEIFKSRALMIATTPIWILTLLFPLRFWPGSQKQIWPTYAILTLLLGHSPVWPLTISWCSANSNSVRTRAVSAAVVNIFSQAGSIISANIYRPDDKPAYKRGNTDLIGVAFGALAMCIVARQYYVLRNRYKEKKWNSFSEQEKLKYIEETTDEGNKRLDFRFSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0K9</accession>
    <name>A0A1E3P0K9_WICAA</name>
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        <recommendedName>
            <fullName evidence="10">Transcription elongation factor 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_79403</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Transcription elongation factor implicated in the maintenance of proper chromatin structure in actively transcribed regions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ELOF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019184885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0K9"/>
    <dbReference type="STRING" id="683960.A0A1E3P0K9"/>
    <dbReference type="GeneID" id="30202131"/>
    <dbReference type="OrthoDB" id="445983at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="Transcription elongation factor 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007808">
        <property type="entry name" value="Elf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038567">
        <property type="entry name" value="T_Elf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934:SF0">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05129">
        <property type="entry name" value="Zn_ribbon_Elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="79"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="107"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ58858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="121" mass="13622" checksum="3D96E5178FB26491" modified="2017-01-18" version="1">MGRKKSSRKPVKKVVQKLDTSFNCLFCNHEKSIICTLDKKNNLGSLYCKICGQNFQTTINSLSKPVDVYTDWYDATEEVNKGHAGSDSDDEGAGYDETTERAKDSNFIEDDDEEDDGDYSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0P4</accession>
    <name>A0A1E3P0P4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peroxin-20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_63420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038120.1">
        <property type="nucleotide sequence ID" value="XM_019183546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0P4"/>
    <dbReference type="STRING" id="683960.A0A1E3P0P4"/>
    <dbReference type="GeneID" id="30200792"/>
    <dbReference type="OrthoDB" id="5407351at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.230">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="35637" checksum="AAB06F13D9DC3116" modified="2017-01-18" version="1">MSLCGPTNALNKLSQHSQVDRSLHQDQQRRFAPSGVDAFKSTQQNNQLELEFQQQQQQHFQQSFNPIFANAFNKPLQRAQPQQAASSSSSWASDFTANQMNQPQQHQYSHGWNNQFQSFQAQHQAQAPAPSIATPRSFTNLAQLSNLSMTQASFNQASTPFRTEHQELHHTNGDLLANELESEFAKLEQEFQNTSLKEEAPVFKRSNSDFANAAMQVVDAMNTNNNEKFKQSKFLSLMNMVAQGKVELNEDETKLVDEAGQDISESTNSLPDPLAGIADGSLETPFQSAQMVGDNHHEVYTWDDVYDDYRHDDSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0Q2</accession>
    <name>A0A1E3P0Q2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Transcription elongation factor SPT5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_84518</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">The SPT4-SPT5 complex mediates both activation and inhibition of transcription elongation, and plays a role in pre-mRNA processing. This complex seems to be important for the stability of the RNA polymerase II elongation machinery on the chromatin template but not for the inherent ability of this machinery to translocate down the gene.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SPT5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037991.1">
        <property type="nucleotide sequence ID" value="XM_019185721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0Q2"/>
    <dbReference type="STRING" id="683960.A0A1E3P0Q2"/>
    <dbReference type="GeneID" id="30202967"/>
    <dbReference type="OrthoDB" id="28901at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032044">
        <property type="term" value="C:DSIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140463">
        <property type="term" value="F:chromatin-protein adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001042">
        <property type="term" value="F:RNA polymerase I core binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001179">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070990">
        <property type="term" value="F:snRNP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003711">
        <property type="term" value="F:transcription elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001208">
        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001209">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000232">
        <property type="term" value="P:regulation of rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090262">
        <property type="term" value="P:regulation of transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06081">
        <property type="entry name" value="KOW_Spt5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06082">
        <property type="entry name" value="KOW_Spt5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06083">
        <property type="entry name" value="KOW_Spt5_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06084">
        <property type="entry name" value="KOW_Spt5_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06085">
        <property type="entry name" value="KOW_Spt5_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09888">
        <property type="entry name" value="NGN_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="entry name" value="NusG, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041973">
        <property type="entry name" value="KOW_Spt5_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041975">
        <property type="entry name" value="KOW_Spt5_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041976">
        <property type="entry name" value="KOW_Spt5_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041977">
        <property type="entry name" value="KOW_Spt5_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041978">
        <property type="entry name" value="KOW_Spt5_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057936">
        <property type="entry name" value="KOWx_Spt5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005100">
        <property type="entry name" value="NGN-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039385">
        <property type="entry name" value="NGN_Euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039659">
        <property type="entry name" value="SPT5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022581">
        <property type="entry name" value="Spt5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017071">
        <property type="entry name" value="TF_Spt5_eukaryote"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11125">
        <property type="entry name" value="SUPPRESSOR OF TY 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11125:SF7">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23042">
        <property type="entry name" value="KOW1_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23284">
        <property type="entry name" value="KOW2_Spt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23291">
        <property type="entry name" value="KOW4_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23290">
        <property type="entry name" value="KOW5_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23037">
        <property type="entry name" value="KOWx_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03439">
        <property type="entry name" value="Spt5-NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11942">
        <property type="entry name" value="Spt5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036945">
        <property type="entry name" value="Spt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="NusG-like N-terminal" evidence="9">
        <location>
            <begin position="203"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="10">
        <location>
            <begin position="290"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="10">
        <location>
            <begin position="438"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="10">
        <location>
            <begin position="489"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="10">
        <location>
            <begin position="611"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="10">
        <location>
            <begin position="704"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="775"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="58"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="135"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="775"/>
            <end position="800"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020181"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR036945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1004" mass="109682" checksum="BE8B9554A325009E" modified="2017-01-18" version="1">MSDDSKEFEHDQANQELHDLEDVKREEDEDAAEEQELNPDEGVLAEHSEEKITGLEDQGNDEDNDDENALDEVDEDVDDDDEDDDEEDEEDRVRSRKRARRDRNQFVDVEAEVSDDDEEEDEDDEELAKEGFIAPEHDHGDEEPISRDDRLHRQVDRTHEKSTEEDAQRLAAQFKERYGRANKYRAEDSGAVSQRFLLPSIHDPSIWAVRCRPGKEKELLTLEGKPNALKILSVFQRDNFTGYVYIEANKLTAVDVAIKGITDFYGSQKVLVPVEEFPDLLRANKSSEIKLTPGSYVRIKRGKYKGDLAIVDSIEESGLEVTLQVVPRLDYKGADIDEETGKRKRVSSKFRPPQRLFSKKEALEFDPTNLTARSSNTFTYKGEEYIDGYLFKLFKVQFLETQNVQPSLEEVSKFNTGDSDDLDLSSIAQSLKNSSSVVFRTGDRVQVLQGEQQGLRGDVISSANDVVLIKPLSFSGGNLEFPTSNLRKIFTEGDHISVLKGKHNGHTGIVVSVNEDQVTFISDQSHKDVTVFANHLTKSTDSSTLIDGKYGLHDLVRINTTTVAVIIRADKDAFTVLTQDGKVSSVSPTAIISKVEVTRDQSFATDSTGEPIKIGDTVKENVGFKRQGVILHIYRSVLFMYSKETTENSGVFVNDISSVSGVASKNNFTEKQDKLDLTRMNPKFRAGGAMAPPPLPVKVQTGRDRTINQNVSVRLGEYKGYRGIVKDTNGDIARVELHTKNKILSISKSKLAFIDNSGRLIPYEEFINPKYRDFSSAASTGSQSSHAPSYSSGGSSSWGSKTPAWSSIGGGKTPIGNNGSRTAVGAHGAGGASAWGGGNNTAWGGNAGGASAWGGAGAGAVGGASAWGGAGGASAWGGGNNTAWGGNAGGASAWGGGQSARKPTTGYGWGGGDSAYGGNGSSHVARNNVLGELKLKLVGTLLHQVAIKLQRLQVITHLLRLQEIIKLKHLMKVPQLLVIMQLQRQETTLMMSDLEILSLGLLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P128</accession>
    <name>A0A1E3P128_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Golgi to ER traffic protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Guided entry of tail-anchored proteins 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GET1</name>
        <name evidence="11" type="ORF">WICANDRAFT_69485</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET2, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3. Within the complex, GET1 and GET2 form a heterotetramer which is stabilized by phosphatidylinositol binding and which binds to the GET3 homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Golgi apparatus membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the WRB/GET1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038312.1">
        <property type="nucleotide sequence ID" value="XM_019184167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P128"/>
    <dbReference type="STRING" id="683960.A0A1E3P128"/>
    <dbReference type="GeneID" id="30201413"/>
    <dbReference type="OrthoDB" id="69461at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03113">
        <property type="entry name" value="Get1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028945">
        <property type="entry name" value="Get1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027538">
        <property type="entry name" value="Get1_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42650:SF1">
        <property type="entry name" value="GUIDED ENTRY OF TAIL-ANCHORED PROTEINS FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42650">
        <property type="entry name" value="TAIL-ANCHORED PROTEIN INSERTION RECEPTOR WRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04420">
        <property type="entry name" value="CHD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="topological domain" description="Lumenal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="9">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="195" mass="22747" checksum="5B7FFF960BF3CEB7" modified="2017-01-18" version="1">MEPYSLLLSILTLLIYSSLLSHFGKQNIIQSIWLIYLKYSSNPKLKQLNKLKTTKKAVFIEKSSISPQDQYAKWTKLNRKFDELNKSIDSLELEIVEFKQNFEKPISLLLSSIYWLPMVWFRIFNRKIGVFWLPNGGFPYYLEKLLSWPSAPIGSIGLSQWCFLINAFLSGVLFIIKNFNVELPEKPTNKITTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1A7</accession>
    <name>A0A1E3P1A7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_63477</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038181.1">
        <property type="nucleotide sequence ID" value="XM_019183555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1A7"/>
    <dbReference type="STRING" id="683960.A0A1E3P1A7"/>
    <dbReference type="GeneID" id="30200801"/>
    <dbReference type="OrthoDB" id="4068815at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014839">
        <property type="entry name" value="Crt10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08728">
        <property type="entry name" value="CRT10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="578" mass="66454" checksum="6671693D8D283077" modified="2017-01-18" version="1">MDPFELLNTNPRQRQYIEHEFSTPPDPSLSPFPPGSDATEEDSPARTIKADKERRFLKYMFLKDTRGHASRHSRCEDADDFYNDNINESQENVAYFKDSYPRTTNSSDLGIEVGVLNAYDIFDVHIPHKLHDFDPESCTNELNYDDALSFKKIRHLEVSRFQSMRYKNNLVESYQDYLFIASKYRILVFKDELQVKTIDVTPSFTTVRHRSAATWSSSPHTINYLKVSKLAGRSVLSCAIDDGRVLIYDIHDFFIKSTHSVPKYELKLSSSVWGVDTYRNMVAVSDNSQTVTLFLFEESGIYHCTSNQIIHNIPTVSFVDIDERNTVYVSCVSISGEFIIFKFETFTNVGPVAQPPYEYSLSIDIVDVLLPMFSKYEFRCTILSRTVLQEDAWATHFIDDKYFKEVTTPDYLGSANYIDVEKILLYSKELNLMSDHLLTSDLGGGAWFEEIGLETPSQRGTATENRLNRFTDKFSRIRKLYFSTSFDEALSPIPKPPYHDKFLFVATASKIGLYRFGNLVCNATSDRLFSYRLSQDLRFSNRLSITKVIPELSAVIVASQAGFFAIFRLFNPMRQLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1D5</accession>
    <name>A0A1E3P1D5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">rRNA-processing protein EFG1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">rRNA-processing protein efg1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_31633</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EFG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037934.1">
        <property type="nucleotide sequence ID" value="XM_019181862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1D5"/>
    <dbReference type="STRING" id="683960.A0A1E3P1D5"/>
    <dbReference type="GeneID" id="30199108"/>
    <dbReference type="OrthoDB" id="47732at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019310">
        <property type="entry name" value="Efg1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050786">
        <property type="entry name" value="EFG1_rRNA-proc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33911">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EFG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33911:SF1">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EFG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10153">
        <property type="entry name" value="Efg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="198"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="227" mass="26090" checksum="96C29EE973F2D4CD" modified="2017-01-18" version="1">MPAIKNPQRRSNGVAVEVASVLGSGTSKISKKIRDIERLLSKRRDKLPANVIVENERALAALKIEKKNATKIQQVKKNAKKYHMVRFFEKKKALRKYKQAKKEYDQLISSNSEKKEIKKAKKKLSHCEIDLAYVVYFPKSCKYIALYPTNSSESEKDETLRKGLLKTEAERNQYKKQYEQMIKDGTILVEDILKGETAATQQSIQDQEDEAPDAAQEQDDAEDDFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1R5</accession>
    <name>A0A1E3P1R5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Large ribosomal subunit protein mL50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_63920</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the mitochondrion-specific ribosomal protein mL50 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038633.1">
        <property type="nucleotide sequence ID" value="XM_019183639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1R5"/>
    <dbReference type="STRING" id="683960.A0A1E3P1R5"/>
    <dbReference type="GeneID" id="30200885"/>
    <dbReference type="OrthoDB" id="3980895at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071986">
        <property type="term" value="C:Ragulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032008">
        <property type="term" value="P:positive regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001919">
        <property type="term" value="P:regulation of receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028209">
        <property type="entry name" value="LAMTOR1/MEH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018305">
        <property type="entry name" value="Ribosomal_m50"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15454">
        <property type="entry name" value="LAMTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10501">
        <property type="entry name" value="Ribosomal_L50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01262">
        <property type="entry name" value="LAMTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="82"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="365" mass="42255" checksum="9CA46F424231B858" modified="2017-01-18" version="1">MGICTSCLRGFTGDENEDTQINETSPLLNNERNYDKDAQLRQREEELNQIVNETNDQLIDISSFYNPNASLSELALKKSINTLKQQQQQQQTQPNDNESKANSQILTSDHNNTVNGEDKSTLNDMHKSMEELIEEDCKIEKRGPLIHTTTPSFGIMDWFDYKKEQKIKQESTKDIIKKVEDDKLEVKKVDKIEFIGVDLDPRDSMTLGERLKGFHIEHWLTKQKISNVESLDEILISTYKGLTNEEISQVSEIKLDDLKLRFDYTLKIQELTGYIIPDYILTKSFNGVILQKFFINEVFSGKIFKKDAESLSHKELQQMDFKSDNIYIHTPVSNTSAKKKYKSLLKRAKKDQELKSEKLIEEARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P242</accession>
    <name>A0A1E3P242_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">glucan 1,3-beta-glucosidase</fullName>
            <ecNumber evidence="9">3.2.1.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_79982</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1-&gt;3)-beta-D-glucans, releasing alpha-glucose.</text>
            <dbReference type="EC" id="3.2.1.58"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.58" evidence="9"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038682.1">
        <property type="nucleotide sequence ID" value="XM_019184982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P242"/>
    <dbReference type="STRING" id="683960.A0A1E3P242"/>
    <dbReference type="GeneID" id="30202228"/>
    <dbReference type="OrthoDB" id="62120at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF1">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE I_II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133627" description="glucan 1,3-beta-glucosidase" evidence="11">
        <location>
            <begin position="19"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="12">
        <location>
            <begin position="85"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="498" mass="58055" checksum="473D314257F0728B" modified="2017-01-18" version="1">MLFNILILSALSLQLCTCFHIKRNLNGSNDVIWDYYDDSKKVQGVSLGGWFVLEPYITPSLFEQFGDDEKKIPVDEYTFTEQLGKDEAQKQLDKHWATYFTESDFKDIKDYGLNLVRIPIGYWAFYLLEDDPYVQGQEPYLDKALEWAKQNDLKVWIDLHGVLGSQNGFDNSGKRGNVTWQDDEENIELSYKTLNYIFGKYGGENLTDTVIGIEIVNEPFHSKLNETDMLDFYYNSYYDFRIKHNSRNFFLIQEAFEPIGFWNTHLNNDYTNVSKPFLNDELLEEGVPKNYFHDIVLDHHHYEVFTVDQLDKSENARIQDIKNYGESVAKEQEYHPSLVGEWSGAITDCAKWLNGVGTGARYDGTFDESQLVRSNAINGTAESQFKFKDKKRSCENVTFVEDFSKQHKENIRKFIEIQLLTYENSNSGWIFWNYKTENAIEWDFKKLVEHKLFPHPFNEYKYFYENGTQIVESAASGNSQNLLFITLSALLVSLSTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2M2</accession>
    <name>A0A1E3P2M2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_63992</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038707.1">
        <property type="nucleotide sequence ID" value="XM_019183650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2M2"/>
    <dbReference type="STRING" id="683960.A0A1E3P2M2"/>
    <dbReference type="GeneID" id="30200896"/>
    <dbReference type="OrthoDB" id="300289at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041545">
        <property type="entry name" value="DUF5601"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045046">
        <property type="entry name" value="Vps9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101:SF25">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN AND VPS9 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101">
        <property type="entry name" value="RAB GDP/GTP EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18151">
        <property type="entry name" value="DUF5601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00167">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="1">
        <location>
            <begin position="120"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="262" mass="30910" checksum="30BE9C92ADB38347" modified="2017-01-18" version="1">MSSIQAYNPEEKPNKFDFQLFLKHLRSRKADPVLRYIRSFLKNFNQKQWTTDEQTKLVMDFKEFIAARMQEYEPFSFMDKHDFENSMFGVEKLVMTKLYEQTYSPEIPSNIIDETHKKDLEYDEILELNYKEYKDLTAKELDVDESIAAKGDYFIKIAGDELNKLNNFKSPRAKVICILNCCKILFELIKKNDQTQNADEFLPLLIYTVLKTQTSNLYSNLAFIERFSFNKSSEVQYYLVSLNAASEFIKNLSREADDNDNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P325</accession>
    <name>A0A1E3P325_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_62191</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038811.1">
        <property type="nucleotide sequence ID" value="XM_019183323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P325"/>
    <dbReference type="GeneID" id="30200569"/>
    <dbReference type="OrthoDB" id="1687175at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="632" mass="71900" checksum="DFF0F7DE0A902C6E" modified="2017-01-18" version="1">MTKVLNHLLENLDSTKTKSIRFPFVKSLHFNEARSINYNIGKFQFPQVSELYFYNFHDVSSFKAISNLELPLLQTMRMTDDSQTISNKKSILHAVKFDKYKRSNDIELVIYDKESNNDVIGSVFNCDLDGVSFDTGFLEVLKISKTLPIRLTLSKSLSSSGIADLMTIVSSTVESLCFVNTSWTSLVWCEGFDPRKLELPNIKTFAMEFSTLTNNSLTFGYPTFERILLSNYSKKVLPMIDYFPFDEYEHLKELVVGSVVQCITVDREIGTRVNPFGAVKSLFLRNVHALEMVNIFTIMNVDAPRLAHLKLEFSGKANTQIRLMNGNAPNLLTVSTSHPNCLSAKELEKLDEPKLEEVDIDETTLKDSSIVFQNLQSLKTCNYHLWHGKFNPSVLVNLEVVFPSATSRSGYAELVNTEFPSLENLDIDVDDAVDLLKFPFNYCFPKVSNLKLSIVYNLSIFFACQLSKFPNLKDLYVVTEDEHIKLDGCQFGLIETLNLFANHAKTFELTNCSFEKLNNFEFCSLLGRYSTHGKLEMYAPCLNNFAVDLDMESIDISKFGSKKLRTISLSGLITDIEHLQSLKIAQPFFKLNYGKEPYYLSSLRLPKSISSLLKDAIEKLEDLAKQKKQRSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P354</accession>
    <name>A0A1E3P354_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U1-C C2H2-type zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_106116</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038529.1">
        <property type="nucleotide sequence ID" value="XM_019180463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P354"/>
    <dbReference type="STRING" id="683960.A0A1E3P354"/>
    <dbReference type="GeneID" id="30197709"/>
    <dbReference type="OrthoDB" id="191651at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013085">
        <property type="entry name" value="U1-CZ_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040023">
        <property type="entry name" value="WBP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13173">
        <property type="entry name" value="WW DOMAIN BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13173:SF10">
        <property type="entry name" value="WW DOMAIN-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06220">
        <property type="entry name" value="zf-U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="U1-C C2H2-type zinc finger" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="74"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="78"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="138"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="166"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="178" mass="20843" checksum="257491B54CE7901E" modified="2017-01-18" version="1">MARYWCKQCEVFVDDNKIARKHHEDSAKHKNSLIRSISKLHQKNKDEHREKFLAEKELREINKELGIKPVFQKRPKVEKKETKSEVSEIEEKSQEPVIGEWSTVETSKEVTPVTPIEKEPENRNSIPKPSKKQSAGDLETKNWDLNTKKQIDTFSSDDEDKPKSTLFKKRKIKKTTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P386</accession>
    <name>A0A1E3P386_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_31346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038985.1">
        <property type="nucleotide sequence ID" value="XM_019181822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P386"/>
    <dbReference type="STRING" id="683960.A0A1E3P386"/>
    <dbReference type="GeneID" id="30199068"/>
    <dbReference type="OrthoDB" id="10257471at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="30"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="582" mass="66516" checksum="321D5CEE3DC3C669" modified="2017-01-18" version="1">MESDTSEFTIRDYNESKHERIQQEHGFEDTSSIHKIPPEVLLLIFSNISHKPDLVPLLLVCRRWANLIVELIWFRPSLMDKGALDGISKVMKMNRQETHWDYRLFIRRLNLSFIYDKVNDNFLNLFSGSTNLERLTLVNCSKLTHEPIVDILQGCEKLQSIDMTGVRDITDEIFAALADNCPRLQGLYAPGCPKVTNPVLLKIIDSCRMLKRVKISDCENLSDDSVLELTMKCKFLIEVDVHNCPNITDFALQRLFNELEQLREFRISHNPNVSDALFRSMAEDTYMERLRIIDFTGCLRITDRTVEKIVQCAPRLRNVVLSKCLNVTDTSLRALATLGKSLHYIHLGHCSSITDYGVSTLIKNCHRLQYIDLACCAQLTNISLVELSLLPRLRRIGLVKCANINDAGILALIQRRGYDDTLERVHLSYCTNIGLYPIYQLLQSCPKLTHLSLTGISAFLRQDITQFCRSPPADFTDHQKGLFCVFSGQGVKLLRDYMTRLFLAPNNMIIPNGLSALGQDGIQGAAQTHLQHFQRLNEFFRQAIPNRQAIDRIPDTETINEVEVPQANPIVQDEDEIMYESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3A7</accession>
    <name>A0A1E3P3A7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_79902</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038589.1">
        <property type="nucleotide sequence ID" value="XM_019184970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3A7"/>
    <dbReference type="GeneID" id="30202216"/>
    <dbReference type="OrthoDB" id="10639847at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="261" mass="29171" checksum="8E323C44C2B4CBF1" modified="2017-01-18" version="1">MSDLTRSLTKVKSKLSSRFKNTANGNDESNCNESQEDQLLAMKYDDFEKQVDNNPSQEMRTSKSFTSLNNIVEVEESEPNQVDDKLLPPVTLDPNTQPLSPSSTSINQMSISNSSPVKTSSLQEKEFSFLQQQQNSSNPTSPINSNMLRNRHDSIIDPLNTGLINEKLNSQILSPQPSSISTTTISSLNNTRRKSSNISNASLVQQPITPTNEVSINQFQLQQENLEFADAMALSILDKVDENIKIEQENQNCSNIDEWTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3B7</accession>
    <name>A0A1E3P3B7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_84976</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lcsJ thioesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038599.1">
        <property type="nucleotide sequence ID" value="XM_019185817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3B7"/>
    <dbReference type="GeneID" id="30203063"/>
    <dbReference type="OrthoDB" id="265761at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00586">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051490">
        <property type="entry name" value="THEM6_lcsJ_thioesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12475">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12475:SF4">
        <property type="entry name" value="PROTEIN THEM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13279">
        <property type="entry name" value="4HBT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="24569" checksum="6347701FF970AC9B" modified="2017-01-18" version="1">MYSIFKYLLYAYLLSSYKSLPLAYYVRFYWQAFKNLILPIYFPSFLDKNSSPFQTKTIHTYNSPFECDIYLHKSNSTYFEELDISRTKLMTLIFQKFFIKYKTESGTWAYVPVANVFTSFKKEIKPYQKYDVKSRILGWDSKWIFILSKFESGQGLHAVSITKYVLKDKRKTIAPVKALEFCGEESRKGVEMVDWFIETGDVEELEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3D6</accession>
    <name>A0A1E3P3D6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_62504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039132.1">
        <property type="nucleotide sequence ID" value="XM_019183384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3D6"/>
    <dbReference type="STRING" id="683960.A0A1E3P3D6"/>
    <dbReference type="GeneID" id="30200630"/>
    <dbReference type="OrthoDB" id="434695at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034044">
        <property type="term" value="C:exomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015374">
        <property type="entry name" value="ChAPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975:SF2">
        <property type="entry name" value="CHITIN BIOSYNTHESIS PROTEIN CHS6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09295">
        <property type="entry name" value="ChAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="688" mass="78265" checksum="F74BAEF1824D9B64" modified="2017-01-18" version="1">MRKIENQSVAPSIGSNTGIAPSGGGTSSPKKKKDSSHSRHHSIQKPIVILPYVSEHSFGGSIGARTANLPNFKELGPPDLVHISKYNKQSQKEEGQYHYITGLDVSSAVAPVAYLTTLNLNSTSSSSKHPNIYTYCSYNAFSRSDVRIRTEFPSSAPGAFQLQLIPSDRSNKILNEVTEEIWDELFVSAVVRSVLINLDFERKVPSLIEKPLTKSVQHSKNIITRLIKFIERGPLLGCNSLIQRPSIVQNYLIDALLKIVELTSLYEFTIDQLQSLTRKDVTLLIVKILFLADREVDAIKLLYEALKKNPRDALLLNEQVKFLISKEKFELALNPALAAVNANPVDFEAWYNLTKLHILNDNIPLALVALNSAPMYGIRPKDLIYIDPKDPITVPYPTEGKIKQVWDNVSQVYGPGCDNSIKFSPRFEVEACDPALIRTNSQQMRGTYRAAYELLILIINQIGWDKLLKSRSKVFIMDDEYKTLSNVSLAADKDSSDDIKKKRLCERWLDNLFLVLYEDLRVVLIVESELQNEKQLKHSGLEWQLIGLASYRTHHFKNAVAALRTQLSAKFDIIAAYKLLELWELNYTTKEFKIWNKTNKYGDFEITLDQILDVLVKCISYNIRFFNEFELPILLFLKKFLNKYDVDFIKNKIQVLFENDNKDYKNSGVIPPFDSLVADIQTLSPDCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3F7</accession>
    <name>A0A1E3P3F7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="16">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">WICANDRAFT_20922</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 16">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="16"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038997.1">
        <property type="nucleotide sequence ID" value="XM_019180890.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P3F7"/>
    <dbReference type="GeneID" id="30198136"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070867">
        <property type="term" value="C:mating projection tip membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990531">
        <property type="term" value="C:phospholipid-translocating ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030140">
        <property type="term" value="C:trans-Golgi network transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140345">
        <property type="term" value="F:phosphatidylcholine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090555">
        <property type="term" value="F:phosphatidylethanolamine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140346">
        <property type="term" value="F:phosphatidylserine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000090">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000172">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF174">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE DNF3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
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        <property type="entry name" value="PhoLip_ATPase_C"/>
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        <property type="entry name" value="PhoLip_ATPase_N"/>
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        <property type="entry name" value="CATATPASE"/>
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    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
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    </dbReference>
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        <property type="entry name" value="HAD-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14 16" id="KW-0067">ATP-binding</keyword>
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            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
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            <begin position="122"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
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            <begin position="71"/>
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        <location>
            <begin position="527"/>
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    <sequence length="1367" mass="155764" checksum="DCAFF0CC0F8B9262" modified="2017-01-18" version="1" fragment="single">RHHSTNKMYAGQRGVIKKKLINLKNFVFNIRPYKKSENGRKIPISIKKEYDHSEYLTLDHKGGELLLDERYKQPYLNNDITSARYSPYTFLPRQLYAQFSKIANIYFLTVAILQMIPSWSTTGTYTTIIPLLIFISISILREGYDDWRRHLQDNKENNQYSRLLQHKSCQRDLHYKDTESLYSLSRTASQNSVSRLDPDEYEQVYNEDLLNQPNTFTKENYLKQMDIMISKVKWKDIKVGDIIQLKQDEPVPADIFVLTSDGSNENEVFIETMDLDGETNLKSRIPHRKISSVMNSAIGLRDFKAGITIEDPNSDLYNFEGNVKIDGNTYPLGPENVIYRGSIIRNTKNLIGLVIFTGEESKIRMNAIKNPRIKAPKLQRYINMIVGFMVLVVLSLSLFSFMAQRLNLKSHWDTSWYLYNQEAGVAPTVMSFIIMYNTLIPLSLYVTTEIIKAVQAGFLHWDIDMYHEPSNTPCEARTATILEELGQVSYVFSDKTGTLTDNLMIFRKFSVAGTSWTHEIDEETDNTKVTTTSNESTSGDEDMNFAPVRSRPSVDIRKSNTVYTGRPSLASALTSNTSKSKTKKETFKTSLDLLKHIQRNPHSLFSKKAKFFILSLALCHTCLPKPVSDDDEEDIEYQASSPDELALVVAARDMGYVVFGKNHKDLTIRTYPEGLDGDPFDEVYQTLDVVEFSSARKRMSIIVKFPDDRICLICKGADNIIMERLKNSDMVTTKQQELYRNTSVRKANEAQVILQQRQSMDVPRKSVQFSRPSISAASVNSNVTSIDDFLHNVKRDEDEIDDVVNEARKSLNLQQKQKYNIDSVGDYLGNDKLVLNEEYVIERTLQHIEEFSTDGLRTLLYGYRWLDKQEYEKWSLDYASAKTSLSDRAKKIEQVGCQLENNLELGGATAIEDKLQDGVADTIEKLRRAGIKLWMLTGDKRETAINIGYSCKLIKDYSTLVILDSNDEDVIAKMNAAELEIKAGNVAHCVVVIDGATLADFESDPSILSIFVTLCTKTDSVICCRASPSQKAMMVSSIRNLNKSLVTLAIGDGANDIAMIQSADIGIGITGKEGLQAARTSDYSIAQFRFLQKLLLVHGRYNYVRTSKFVLSTFYKELLFYLTQAIFQRNTMFTGTSLYEPWSLSMFNTLFTSLPVLCIGMFEKDLKPATLLAVPELYAKGRLNQAFNLRIFIGWMVVAASTSVLICFTSWYLWGYTALIDNSLYPCGVLTFTAIVFTINIKLQFLEQHDRTSMNLASALISCLGWLLWCCLLPGLYKPETSKIYGVLKGLYHGFGSDVTWWASLLIIVSMPLLYDIILQVLRNWFFPTDSDVFRVLEKRTEIRRKIEIEAYDQMHQGWSWSKDESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3P8</accession>
    <name>A0A1E3P3P8_WICAA</name>
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        <name evidence="2" type="ORF">WICANDRAFT_91873</name>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
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            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
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    <sequence length="314" mass="34669" checksum="E415EAD790B5EBED" modified="2017-01-18" version="1" fragment="single">MSEEKRPLSNDDIIMDLISEIKVEKTLLQSNIDEIVDDGAGCIDPQLLSVNNEELVEDGDLTEGGNDLEVVHFAKVSENGDISKDGDDSTLDGTELYTALHFDGSNVDLKKPQILLPTTTLPLNNGLGNPTNDDGENDDDQSVSPSVSELFEWSLQEEAPLRLDIVQYDESEPEFEPESNEQPADSQFQEVLASQIDQNNSQRYHIDGSIGDQVFPDSFKISFSTNMNSNSSIGESILHRDGSLLGQDSVLEEELNSVSRGALMHDWNEDMGSLNQLKSIPTRRTVAYSKSNSPLEEPNQDNIKPQTSTPKLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3Q8</accession>
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            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
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        <lineage>
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            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
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        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of proteolysis"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46803">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46803:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHIP"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0808">Transferase</keyword>
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    </evidence>
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        </source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="292" mass="34043" checksum="EB032DC4C069B058" modified="2017-01-18" version="1">MSSSNKTAEKFKTKGNRAFASEDYDEAIKLYSSAIQHDSTKANYFTNRALARLKSSKAEQQYDLIAKDAEKALELTPDNSKAFFYLGIALTKLDNPELALKKLNKAYQLALETSNKSAQQISEQILETRKEIAKKEFNERISRTNPLYEKLYRLLQEFYWSKINYLRSRFNYKPFYEDDIDFQIEYKSLKDQYIRDLDDLRHTFEASLAKDRAPPAKEEAPEYILDPIGFNIFNDPVVTPSGFTYERSWIVEHLKTSPADPFTRAPLRESDLYPNLAVKKAADKYIEEHRHL</sequence>
</entry>
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    <accession>A0A1E3P3Y6</accession>
    <name>A0A1E3P3Y6_WICAA</name>
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            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">WICANDRAFT_62771</name>
    </gene>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="108" mass="12511" checksum="8821A12938F4C4F3" modified="2017-01-18" version="1">MSGNRLDGIDKERNIEILEQLNERLELNTRLLELQQEFSAIKEGLKNDVREYEEAERNLLESNKELEEHLQPQLDALELLIKANGGEVEIDDDESDENDESDENDADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P470</accession>
    <name>A0A1E3P470_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
    <gene>
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    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
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        <lineage>
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            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="53"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="320" mass="35788" checksum="964A8087C84F0266" modified="2017-01-18" version="1">MNFEQSYLDDFMNNDYINIDLDKFSIPLTGSEDLLSQSDFNNELYQIISNNSSISTPSSSGSFSSATSSNYNSLTRTSTTTPAPTHTLGFSEAENNIYSTKLEESLIDLNSMIKDSGSTLNYLDYSTYSSPLETNSIAASPANLLEFKFQTQQLSASRPQTPANIAMKVNNTPQTPALTHQDASIQSRNSSISSQSSSSQTHSSSSSKKNLDSRLSLQKLGELLNTSSIEETMKIEKFILDIFGKELGFPLGYKTWIRDTNEEYRKYLINELHTRASKVYPKLTKSLLETVIKRATYSMMQGRLRKERRAALKAKRSVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P498</accession>
    <name>A0A1E3P498_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aldehyde dehydrogenase 5, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_30064</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Minor mitochondrial aldehyde dehydrogenase isoform. Plays a role in regulation or biosynthesis of electron transport chain components. Involved in the biosynthesis of acetate during anaerobic growth on glucose.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039287.1">
        <property type="nucleotide sequence ID" value="XM_019181683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P498"/>
    <dbReference type="STRING" id="683960.A0A1E3P498"/>
    <dbReference type="GeneID" id="30198929"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019413">
        <property type="term" value="P:acetate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07091">
        <property type="entry name" value="ALDH_F1-2_Ald2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000011">
        <property type="entry name" value="ALD5p Mitochondrial aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000001">
        <property type="entry name" value="Mitochondrial aldehyde dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="54"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00053679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00069627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ60080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="523" mass="57235" checksum="7E8391ED073997EB" modified="2017-01-18" version="1">MFRFQTTKTALRNVSLLSLKASRNYSTIPLSVPITLPNGITYDQPTGLFIDGKFVASRQHKTFEVINPSTEEEITHVYEAREDDVEIAVEAAQRAFDSSWSTSDPEFRGRVLYKLAELIDQHAETLAGIESLDNGKSLLCARYDVALVAKYIRSCAGWTDKVYGRVIETGDSHFNYSRREPIGVCGQIIPWNFPLLMWSWKIGPALATGNTVVLKPAEATPLSALYVSKLVAEAGIPAGVVNIVPGFGKIVGEKISTHPKIKKVAFTGSTATGRHIMKAAAESNLKKVTLELGGKSPNIVFNDADIKQTVQSLITGIFFNSGEICCAGSRVYVQDGIYDEVLKEFKSFAEGLKIGNPFEDGVFQGAQTSQMQVDKILQYVDIGKSEGATLISGGERLNQKGYFIKPTIFADVKEDSRIVKEEIFGPVVTVSKFSTVEEAIKLANDTNYGLAAGIHTTDINKAIDVSNRVKAGTVWINTYNDFHQQVPFGGYGESGIGREMGADALDNYTQVKGVRIAIQKKQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4C0</accession>
    <name>A0A1E3P4C0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">General negative regulator of transcription subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_78666</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039251.1">
        <property type="nucleotide sequence ID" value="XM_019184769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4C0"/>
    <dbReference type="STRING" id="683960.A0A1E3P4C0"/>
    <dbReference type="GeneID" id="30202015"/>
    <dbReference type="OrthoDB" id="293823at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012270">
        <property type="entry name" value="CCR4-NOT_su3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007207">
        <property type="entry name" value="Not_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04065">
        <property type="entry name" value="Not3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005290">
        <property type="entry name" value="NOT_su_3_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0678">Repressor</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-Not complex component Not N-terminal" evidence="13">
        <location>
            <begin position="2"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="14">
        <location>
            <begin position="440"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="259"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="333"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="116"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="274"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="300"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="353"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="386"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ60044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="579" mass="65750" checksum="8158DF95FF7AD91A" modified="2017-01-18" version="1">MSQRKLLQEIDKVFKKVKEGLEIFDGYYDKLQNCESQSQKEKIEGDLKREIKKLQRQRDQIKNWLSGNEVKDKKNLLENRRLIENAMERFKTVEKDMKTKAFSKEGLSMQRIDPKQKEKNESSDFLHQMLEELERQCEKHEATIDQIQSSGKKGKKLDGAKQQEIEELTKKISRNHWHQEKMEQMLRLLANDDIECEQVNGLQEDIKYYVESNEDADFAEDEGIYDELGLEDIEDMYGHVGDYPQAAPEDEIEQEAPIVDPSPQKKPSVSSHPSATNVAASASSTSVNTISKLPPPGLPQPSTTSSTTNLKPSVTPLKTDLKYSSVLSAGVQSNNNTPIATQAPSISTPPISNAALNNNTNNNNFNHNNISSTAASSPQVAPPGLTKTSSITTDQSRETTQSPVLIPSGFQDLIPSFEKSKQNISKPPPLDEISKILETSLLNCPDSLDADKPKTYSPLNPHPTSVYYPQEPLPELSYSSIVSKLDLSTLFYNFYYEQNQYIQILSARELVKKGWAFDKLSKHWFQEVEEIQPPNIVNDQIIHSEKKIAWKYFDNDGVWLPRRKEDVFNPEQVQLETNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4I8</accession>
    <name>A0A1E3P4I8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL23m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_30363</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039331.1">
        <property type="nucleotide sequence ID" value="XM_019181725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4I8"/>
    <dbReference type="STRING" id="683960.A0A1E3P4I8"/>
    <dbReference type="GeneID" id="30198971"/>
    <dbReference type="OrthoDB" id="275582at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000614">
        <property type="entry name" value="Mrp20p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12059:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL23M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12059">
        <property type="entry name" value="RIBOSOMAL PROTEIN L23-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="247" mass="28625" checksum="0A3F8DD54418852F" modified="2017-01-18" version="1">MPRLINQLLAKTVFSSSAKRSLATVSPKTITTTKKPEQPSTTKNPTFPQKIYTLIQKSIAKGEAHFKVGGKKLYFPTARVVLLRPNAKHTPYQAKFIVPKSFNKMDLRDYLYHLYGIRAFKITTQLIAGKYTREGAIARYRTPQIKKMTVDLLEPFIWPEEPENKKEEFNIKFMEEMRLYGEESRRIGSDRFKPGKAFDGELGPYPHKPEPFVPKFLKKNLINERNKDQAKIDRADNEKLVSKYLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4M3</accession>
    <name>A0A1E3P4M3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Quinate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_92343</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039371.1">
        <property type="nucleotide sequence ID" value="XM_019186355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4M3"/>
    <dbReference type="STRING" id="683960.A0A1E3P4M3"/>
    <dbReference type="GeneID" id="30203601"/>
    <dbReference type="OrthoDB" id="508119at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000026">
        <property type="entry name" value="MFS quinate transporter QutD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF34">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0672">Quinate metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="458"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="22"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ60164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="560" mass="62164" checksum="2F07E9AB297DA8A8" modified="2017-01-18" version="1">MFAKHETRPTPQVVYNWRIYWAACLASWVAVIIGYDAGFVGGMIALKSFKKEFGFDKMTPSEQSWVSETVVSLFQAGAFFGAFLIYPLGSKYGRKISLAVASVLLIVGSAIQLASNSKTGLAAMYAGRVLTGLGIGAVSNLAPMYSAEVSPDAIRGRLVGLFEISWQIGGTIGFWINYGTSVNIPDDEAKQWQIPVALQLIPAGIFGIGLYTLVESPRWLFTVGKREQALKNLCYLRQLDPEDEYIKYEVAIMEEEVEQKRAKIGLGLWDPFIKLFRSSSLRYRLLLSTSTFIIQNTLAVNAVNYYSPRIFQTMGISSLDSSLLSTGIFGILKGVFCLVWSLFIVDRFGRRPSFIFGLVVCSLCMWYIGGYIKIGKPTSRGEDAGLDAGGKASLALFYIWTIAYAVSWSGTPWVWNTEVFPSNLKSATSSVNAASNWFWAFIMARFTNQMIDKMKYGIFFFFAAMMTVCLPIFFLLYPETKNIPVEYVDELFRHKAWRAHGEVMKLIAEGKENDLEEGQNVLMGVNASSRDLESKGIEKPDVEIVSMVGSSQGDAASKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4P1</accession>
    <name>A0A1E3P4P1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mRNA decay factor PAT1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_79047</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF09770">
        <property type="entry name" value="PAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="mRNA decay factor PAT1" evidence="2">
        <location>
            <begin position="7"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="148" mass="16309" checksum="B730B80A81D7C934" modified="2017-01-18" version="1">MSYRDFKNNKDVLQFEDTYEGLDEEDDALNDETFGVDVPVGQAFDFGNPSESQPQPQLNFAQAARAPPQQISIQEPLGDDPLKPIESLWGGAPPVAPQQQQQQQQPKVLSLDELESQLTQNPASSQVISYSPVNRSLFILVSSSSETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P588</accession>
    <name>A0A1E3P588_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_62630</name>
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    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039269.1">
        <property type="nucleotide sequence ID" value="XM_019183407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P588"/>
    <dbReference type="STRING" id="683960.A0A1E3P588"/>
    <dbReference type="GeneID" id="30200653"/>
    <dbReference type="OrthoDB" id="186013at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyt_c_oxidase_su4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036639">
        <property type="entry name" value="Cyt_c_oxidase_su4_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707:SF9">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
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    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="136" mass="15962" checksum="38F85C71C13C6059" modified="2017-01-18" version="1">MLMIKRSARVINSPILIRSLTTTQEYVPPDIKGLEKRWEKMKELDQADVIDYLNWKGQDDWRKLSDQEKKSWYYIYYGNWGPRSSTPQQSISGTVLRALFGGVLTIALGVSVMNYAYDLEREEKVKNLLERIEKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5T1</accession>
    <name>A0A1E3P5T1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome c oxidase assembly protein COX16, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Cytochrome c oxidase assembly protein cox16, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_28163</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the assembly of the mitochondrial respiratory chain complex IV (CIV), also known as cytochrome c oxidase. May participate in merging the COX1 and COX2 assembly lines.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the COX16 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040008.1">
        <property type="nucleotide sequence ID" value="XM_019181454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5T1"/>
    <dbReference type="STRING" id="683960.A0A1E3P5T1"/>
    <dbReference type="GeneID" id="30198700"/>
    <dbReference type="OrthoDB" id="5516033at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020164">
        <property type="entry name" value="Cyt_c_Oxase_assmbl_COX16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17130:SF14">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX16 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17130">
        <property type="entry name" value="MITOCHONDRIAL OUTER MEMBRANE PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14138">
        <property type="entry name" value="COX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ60801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="119" mass="14066" checksum="68E534E475614610" modified="2017-01-18" version="1">MGELDSKKFRSKRQQAAYDASLAGRYQKALKKNSFLMFGLPFLTFMGLASYWFSSFTAIRYEQRDKKVQEISEEEILNISSKRRTVDVKEEYYRLQGLGEEDWEPKRVPRMKGESENVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5T9</accession>
    <name>A0A1E3P5T9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartate aminotransferase</fullName>
            <ecNumber evidence="7">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_30060</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="7"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039509.1">
        <property type="nucleotide sequence ID" value="XM_019181681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5T9"/>
    <dbReference type="STRING" id="683960.A0A1E3P5T9"/>
    <dbReference type="GeneID" id="30198927"/>
    <dbReference type="OrthoDB" id="6752799at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006532">
        <property type="term" value="P:aspartate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032938">
        <property type="term" value="P:negative regulation of translation in response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000064">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000001">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF55">
        <property type="entry name" value="GLUTAMATE OXALOACETATE TRANSAMINASE 1, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="8">
        <location>
            <begin position="30"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="410" mass="45457" checksum="B2ABBA74661ABE93" modified="2017-01-18" version="1">MSHIFASIEQLPPDALFGLKARFVADSRPQKVDLGIGAYRDENGKPWILPSVKEADKLIHEDPAFNHEYLPIQGFASFVSAAAKVILGDESPAIKEDRLVSIQTLSGTGSLHIAAKFISKFYKKSQTVYLSRPTWANHFQVFESLGLKTASYSYWNDETKSLDIEGYLKDINNAPEGSIFLLHACAHNPTGLDPTREEWVKILDAIKAKNHLPLFDSAYQGFASGDLENDAWAIQKGVEVLETPILICQSFAKNVGMYGERIGAFHLVLPTPDNKPAILSQLQVFIRSELSNPPAYGAKIVSKILTTPSLRNQWKQDLVTMSQRIYSQRVALRDLLVKLGTPGNWEHIVKQQGMFSFTGLNKEQVARLESKHAVYLVSSGRASVAGLNSSNVEYVAKAIDEVVRNTSTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6A6</accession>
    <name>A0A1E3P6A6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_61451</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040095.1">
        <property type="nucleotide sequence ID" value="XM_019183177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6A6"/>
    <dbReference type="STRING" id="683960.A0A1E3P6A6"/>
    <dbReference type="GeneID" id="30200423"/>
    <dbReference type="OrthoDB" id="286814at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF36">
        <property type="entry name" value="PHO85 CYCLIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="27624" checksum="95D791BCA55EB2D0" modified="2017-01-18" version="1">MLNLTPENDSQVNHYQHHQNVQLITPPSSAKQSNISSEKSHKADIVNKYITEASVFILSFWKNPQQNSLFNTQTNLINQKDYERFTREVLKRSRCSLLGLQVAMYYLFKLQKNIKQRFISNDKSLMCPKRTFLICLILSFKFNYDSNYSFKSWSKISGLSIGEIKTLEFNILQSLDYDLNCNVETFEKWSLLLNQALEKIEKLIDNRNSNTTSLKRTCTSIEEENNNIKRVKIQTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6C2</accession>
    <name>A0A1E3P6C2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Calcofluor white hypersensitive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_100546</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039604.1">
        <property type="nucleotide sequence ID" value="XM_019180298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6C2"/>
    <dbReference type="STRING" id="683960.A0A1E3P6C2"/>
    <dbReference type="GeneID" id="30197544"/>
    <dbReference type="OrthoDB" id="68581at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000031">
        <property type="entry name" value="Calcofluor white hypersensitive protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019402">
        <property type="entry name" value="CWH43_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
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        <property type="entry name" value="GPI-anchor_lipid_remodeler"/>
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        <property type="entry name" value="PGAP2IP_TM_1nd"/>
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        <property type="entry name" value="PGAP2IP_TM_2nd"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PGAP2-INTERACTING PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="294"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="445"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="485"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="512"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="534"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="564"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="599"/>
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    </feature>
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        <location>
            <begin position="628"/>
            <end position="648"/>
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    </feature>
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        <location>
            <begin position="10"/>
            <end position="219"/>
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        <location>
            <begin position="272"/>
            <end position="425"/>
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    </feature>
    <feature type="domain" description="PGAP2IP second transmembrane" evidence="3">
        <location>
            <begin position="448"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="PGAP2IP C-terminal nuclease-like" evidence="5">
        <location>
            <begin position="663"/>
            <end position="905"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="933" mass="104984" checksum="DF780BE94D55A282" modified="2017-01-18" version="1">MSSSIISINAKYISWAHTICASSAFIAALIVGVCLHYHKIVENAHYGYPDEWFPSVSATIGDRYPERSIFQILIALTSGPRFLLLLLSYIRLYDENHKRFGIFGVVCGVVRTVTCGGWVYITSTDDHDAHDVFMISYIVLTIPWEYYVITSTPKRTSLRNYRFYTSVAFFTMLIPLIYFFIQHKVHRIAGAYSIYAYFEWSLIILDVAFDAWTAVDFQDLTITLSASKPISFSIDQNEGTIVDEIKENTDLKPKSRDDIPFLELIINTINSFIFWSVATSLLTTIWFFPLWNMGISGFELTILSFLFSFLLIIPPVKRCFASYPQISRLLTTLFGVGSFKVEDPDTRLLLIAVGSAFGAVSLANETHHYGSNNTRARYYSTTFTLGLILSSIAKFSNYTNNPIWPIMHAENGGWNNTGVLIGVIGSILTPRVNHVTSINSQKNKFGLITTGLGLGGLIFSLHAMLTDSSTIIIWNWEGYPINGPVPVPHGAITLIMMGVGSYLGLTQDLNTKLVSIGGVVSAIVLYIATEWIGYIGGVGYILFLTSVVPFFFNNASKYQPGVVFTLAFFVHIVLMLAHVWIVAYAFVPAGWLLRERTDIVLGVSTGLIVLGLISNFKDDVVVNFKPALYKIKRILIIFSITSVLIAYLRFPKTDPTPYHPNSKLITAGIWTIHFGLDNDMWASEGRMIDLIRDAELDIVGLLESDTQRIIMGNRDLTQQMSEELGMYADFGPGPNKHTWGCALLSKFPILNSTHHLLPSPVGELAPAIHATLDVYGELVDVVVFHSGQEEDAEDRYLQSMGVRDIMGSTDRPMILLSYLVTTPLEGNYNNYVSKESGMHDIDPSDWDRWCEYILYKNLKRTGYARISRGSITDTELQVGKFQLPNNKDEAKEYSQERIEEGKVDEDLRFPNKFYGDGVRDHRFHVFDEPRYFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6H4</accession>
    <name>A0A1E3P6H4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_104160</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ61019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_019180401.1"/>
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    <dbReference type="STRING" id="683960.A0A1E3P6H4"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061700">
        <property type="term" value="C:GATOR2 complex"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:positive regulation of macroautophagy"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of TORC1 signaling"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
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        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037590">
        <property type="entry name" value="WDR24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46200">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46200:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR24"/>
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    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0853">WD repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="181"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="270"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="37"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="449"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="707"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="54"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="452"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="470"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="716"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="773"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="963" mass="108153" checksum="E47A6B41668EC611" modified="2017-01-18" version="1">MSSYDNRYGTSEGSQFGSSSRHSNTLARLVNFYGNSGSTTTSSISPSRVPTPYTPSRQSPPVFTPYNESTSNYEDINQTRFKTTQELTSLSRINNDSSDSVVIAGKNLLQILKIENSTITPVNDLLSSSYYLKNKKVIGSILDVEAGYHNHSKFIATSTTTGSIFIFNNTSQSKNKLVLKYSDHTRAVNSISFSQLDSHSLLSGSQDGAMKLWDLRSNLTKPVFTMIGNSDAVRSLKFSPFHNKKMCAIFDSGVIQKWDLRVPTQFERKFNAHTGPGLSIDWHPELDYIVSGGRDKQVQVWNMNNNDARQLPEHVIYTAAPVNNVCFRPNSNGVSNIMNAEVATSFLTNDLSVQIYSLKRKYIPEYTIESHHAQISGLVFKDERTLLTCSKDKSLVRDDLNAYYHPIDNLPINATSWSVNDDLVFINQEREPKELEPILESNNQYSGFNPISSMMSTSPNNSPGNSAGTTAVNTPSVSSFPDLSPRPGISRSSSTALRPSVNRALSHGSHHNNVSNYSNPFVVPISLPISKSNASLKYLSDNYMFDPSHDLIMSCEINASVANDVDCLRDSQTWKVIKESLIWETQLENELKYELDGTTEVEINTTAGHDYETDNEKDYHYSTSNSTTNYGKSPALSDLSEFNSLNSKEMSLRSKEDLKITKARKSFDEIQEKGEEVSDDQEEVDKNKTVTKEDILKWDDLPVKQVTEKDDTVTKEEDDEEEQQGEEEEAIEFVESKATSKPISLPGKRKPRSSILEAILPPNSDESNKIGKSSSPSYSITSSFSNASNSLRPLTQSLAPLLHKSNFNKLTKQDSHKSELTRQLTNQKQEKEADNKLILPPYAPKFLIKKAIDFSLQQGDLIMSCTLLLLFNDRYKLYGDKIVKDMIHSYLEFLKKLQFFITFTKIISISKYNEFKEMSLNSTILRRFCSKCGVLIVNENLKEAVLKDPKIEFVDILDVSKHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6J3</accession>
    <name>A0A1E3P6J3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_77701</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040253.1">
        <property type="nucleotide sequence ID" value="XM_019184595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6J3"/>
    <dbReference type="GeneID" id="30201841"/>
    <dbReference type="OrthoDB" id="4067912at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="232"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="99"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="258"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="277" mass="31756" checksum="A90956C3A4D5AB62" modified="2017-01-18" version="1">MSVSENYIPQSENLEHFNNIVDELRSITDENTQLKNNILTKVDLLIKQLNHGHVDNSDVIRKLNGFIAQFGGDEVAKSNNGTGDVMDEKTQLLEQNKKLKDILASKIKYNDDIEQLNKEYQEGIDQVMRDVRIRKFHSDHLRIENFKKIHGSIAQLQDEEFQTYLILINHQEILSKIGRAISDVLKNLNQDSRDELELQKLLTTLELYKSSCLNKNVAAVTTQRFKTLTKKFKNNGGKNNSMQNLSNGNGYINYIPPNKRDPAPSFDKESEWPPLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6R1</accession>
    <name>A0A1E3P6R1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA-5-taurinomethyluridine 2-sulfurtransferase</fullName>
            <ecNumber evidence="3">2.8.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_28017</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5-taurinomethyluridine(34) in tRNA + S-sulfanyl-L-cysteinyl-[protein] + AH2 + ATP = 5-taurinomethyl-2-thiouridine(34) in tRNA + L-cysteinyl-[protein] + A + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47040"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11726"/>
            <dbReference type="Rhea" id="RHEA-COMP:11732"/>
            <dbReference type="Rhea" id="RHEA-COMP:11733"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:87171"/>
            <dbReference type="ChEBI" id="CHEBI:87172"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MnmA/TRMU family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.14" evidence="3"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040245.1">
        <property type="nucleotide sequence ID" value="XM_019181440.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6R1"/>
    <dbReference type="STRING" id="683960.A0A1E3P6R1"/>
    <dbReference type="GeneID" id="30198686"/>
    <dbReference type="OrthoDB" id="3685at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01998">
        <property type="entry name" value="MnmA_TRMU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.280:FF:000001">
        <property type="entry name" value="tRNA-specific 2-thiouridylase MnmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000115">
        <property type="entry name" value="tRNA-specific 2-thiouridylase MnmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.280">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004506">
        <property type="entry name" value="MnmA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046885">
        <property type="entry name" value="MnmA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046884">
        <property type="entry name" value="MnmA-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023382">
        <property type="entry name" value="MnmA-like_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001138">
        <property type="entry name" value="PRK00143.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00420">
        <property type="entry name" value="trmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11933:SF5">
        <property type="entry name" value="MITOCHONDRIAL TRNA-SPECIFIC 2-THIOURIDYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11933">
        <property type="entry name" value="TRNA 5-METHYLAMINOMETHYL-2-THIOURIDYLATE -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03054">
        <property type="entry name" value="tRNA_Me_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20258">
        <property type="entry name" value="tRNA_Me_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20259">
        <property type="entry name" value="tRNA_Me_trans_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA-specific 2-thiouridylase MnmA-like central" evidence="13">
        <location>
            <begin position="201"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-specific 2-thiouridylase MnmA-like C-terminal" evidence="12">
        <location>
            <begin position="296"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ61038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="360" mass="41345" checksum="96D0A5EFD8BEF7E5" modified="2017-01-18" version="1">MSSGVDSSVAAALYSKEYKNCTAIFMENWSQSDTSRCLEQDWKDAQAVAQQLDLPIEKTNFQKEYWIDVFEPMLGEYNKGITPNPDINCNRYVKFGKLYEYVSEKYKGQNWWLVTGHYSRILQRQDTGEHHLMRPYYKRKDQSYYLSQIDPSVMKRILLPIGHFTKPEVREMAEQLQLEVASKPDSQGLCFISQGEGKFNSFLKNFLNESPGNYITEDGKIWGKHEGLWSATIGQRCRLEMPQGDPNYKGTWFVGAKNFETNDITIVRGTDNPKLFKQIVYVKDFYPLDPNYDLVSNVPNLSVQYRSLQKAEKLDSIVKTDEGGLIFQLGEKRRAIAPGQYLAVYDGDRCIGSGVIEASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6X0</accession>
    <name>A0A1E3P6X0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA topoisomerase I</fullName>
            <ecNumber evidence="7">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">DNA topoisomerase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_99725</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6 7">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the type IB topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="7"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040372.1">
        <property type="nucleotide sequence ID" value="XM_019186695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6X0"/>
    <dbReference type="STRING" id="683960.A0A1E3P6X0"/>
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        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
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        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="TopoIB_AS"/>
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        <property type="entry name" value="Topoisomer_I_DNA-bd"/>
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        <property type="entry name" value="DNA TOPOISOMERASE 1"/>
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        <property type="entry name" value="EUTPISMRASEI"/>
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        <property type="entry name" value="TOPEUc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
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        <property type="entry name" value="Eukaryotic DNA topoisomerase I, N-terminal DNA-binding fragment"/>
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        <property type="entry name" value="TOPO_IB_2"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="DNA topoisomerase I eukaryotic-type" evidence="10">
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            <end position="726"/>
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    <feature type="region of interest" description="Disordered" evidence="9">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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        <source>
            <dbReference type="SMART" id="SM00435"/>
        </source>
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    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ61165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="754" mass="87423" checksum="856C43F6D641353F" modified="2017-01-18" version="1">MSDSEDDIVLARRTKKQPSLAANESDEDDVPLSQLKKESSPDDTSISSNSQKSASKNSKASKRKTPSTSSQPKKRVKKEVKDEEEDDDDDDKPAKAPAKKPVKKEETPNSDEPEEETYKWWEDQKDDDTFKWDTLEHHGVMFPPPYEPLPSHVKLYYDGKPVDLPPDAEEVAGFYAALVETDHGKNPVFQKNFFNDFLQVLSESGGCNVDIKEFEKCDFSKMYNYFELKKEEKKNVPAFEKKRIKLEKEKEEEPYKFCLLNGRKEQVGNFRVEPPGLFRGRGAHPRTGKLKRRVHPEDIILNLGENAAVPPPPEGHNWGEIKHDNTVSWLAMWRENVLNSVKYVRFAANSSLKGMSDYKKFEKARELKYHIEAIRKDYQSMLRDKLMLNRQIATATYLIDIFALRAGGEKSEDEADTVGCCSLRYEHITLSPPNTVVFDFLGKDSIRFYQEVQVDHQVFKNLKLFKKAPKKPGDELFDRLDPSILNKRFQNYMPGLTAKVFRTYNASKTMQDQLDLIPNEGTPAEKVVKFNAANRTVAILCNHQRSVAKGHEAAIQKITDRIKEMLWQKIRFKRMMLQLDKNLKKKKPKYFEELEDLTKEDELEIHKVIIERERDKQHKKFARDNEKRKEDGEKLLPESELKEALKKVDELEKEYAKEAKSGKPEVKPANTTVEKLEAQVEKLEQRIANTTLQLKDKEDNSEVALGTSKINYIDPRLSVVFCKKFDVPIEKIFTKTLRDKFKWAIESVDEDWRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7D2</accession>
    <name>A0A1E3P7D2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_18713</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ61336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001965">
        <property type="term" value="F:G-protein alpha-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043577">
        <property type="term" value="P:chemotropism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045141">
        <property type="term" value="P:meiotic telomere clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
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        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="129"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="513"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="597"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="670"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="740"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="815"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="895"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="964"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="1075"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="997"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1000"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1035"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1138" mass="125190" checksum="38E80F85C6407D1E" modified="2017-01-18" version="1" fragment="single">VAAPPPSINDESAFPALGGSAGSASGSNVIWGPSMKTPSSASSSDKVFSRSSSSRVQKSVQEAFNIKNNVSLNVSTVEFRKIISELKKTYDVSLESTVSTINKDRTFVVSGSIKNVAQVRKELVRRLTKPVQDRFKIPSRTRAAVIGSGGKNLRPIIESTGTRINIERNVTPGPQSSQSDEDDEIEVTIDGDIDGVTDARKLILAIVDEETKHLSTRVQVPDELIKFVKPFDINEENLKVSGPNKNGSISIQGLRDDVLIKKNEIVSQLNDLKIKIKTETKSIPKKVHQFIKPDEIYKQFNVVIEIPKGEIDDEELVSFVGLPSDINAAIQHARNTTSQFIIDSLDISRAHGGNVDHARALAAYFSHAGLLNKIGEEFNTITSAPPFDKLVSRNLKNVSIDISAPKTSTQSIKDSRKKIVELVNGLAPTRIRFVNDVVPFFAKRVASITESSSKSQNVHVVPLSFLAENKTNEIILVALDNEDDEFAPSQEEINARLDVVDHSLDELRKAQSELKSVVLDVENDKQQFIEGPNGTTLKTLLNSFNDGSSAIVLKLHYNGDLPSPDKVYIQGTKTDVGKVEKEIQALLKDAEDLKDIYSFKSEIHVPKNVLPRLIGKNGGHLNTLRDQFGVNIDVEKDPQGEKAALTITGYKFNVKEAEHDILQSSKRWADEVTKTVIVPQKYHGSIIGQGGQYVKRLQDKYNVRINFTQGVDDVVIRGPSRGAIKAEEEVKELVDYFIENGYTKEIQVPTKALSRIIGKNGETMNGIAADAGVEIDVKDNDDKESEFGSVLLTGSRKGLKEAETKILAIVKEFQDTITVELEVDPKYFRDILGARGSTKTAIIEKAGGADLDQQRRLLQIPDQSSESKIITSSGPKKVVESIIEQVKKIVQEKENSVTEKLDVSKDKHRLIIGTGGTVRRTLETGHKVSINVPKFASDSSEVTIVGLPENVEKAKAAILELTADDWKTVVEVPAYLHAAVAERGAFTRKIRSDHNVEVTHGNSSNRAYKLSSSHIPTPPSSASGSDEETFKFTTEDADSSADDDSDLIPWRLKGEDEDVAKVESLIKARIEQFKKDNTSGFLWYKDPSVFGRIVGIQGSRLNNIRKKSGAQIYIPRKDDTANDVIYLKGTKAALEKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7X3</accession>
    <name>A0A1E3P7X3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Transcription initiation factor IIA subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_28045</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">TFIIA is a heterodimer composed of the large TOA1 and the small TOA2 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TFIIA subunit 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040617.1">
        <property type="nucleotide sequence ID" value="XM_019181442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7X3"/>
    <dbReference type="STRING" id="683960.A0A1E3P7X3"/>
    <dbReference type="GeneID" id="30198688"/>
    <dbReference type="OrthoDB" id="586585at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000979">
        <property type="term" value="F:RNA polymerase II core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10014">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10145">
        <property type="entry name" value="TFIIA_gamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.190:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIA subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.18.10:FF:000003">
        <property type="entry name" value="Transcription initiation factor IIA subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.190">
        <property type="entry name" value="Transcription factor IIA gamma subunit, alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009083">
        <property type="entry name" value="TFIIA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003194">
        <property type="entry name" value="TFIIA_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015871">
        <property type="entry name" value="TFIIA_gsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015872">
        <property type="entry name" value="TFIIA_gsu_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10966">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02751">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02268">
        <property type="entry name" value="TFIIA_gamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009415">
        <property type="entry name" value="Hum_TFIIA_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47396">
        <property type="entry name" value="Transcription factor IIA (TFIIA), alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit N-terminal" evidence="10">
        <location>
            <begin position="6"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit C-terminal" evidence="11">
        <location>
            <begin position="63"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR009415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ61410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="111" mass="12701" checksum="8CDF16AB0C759722" modified="2017-01-18" version="1">MSSQGYYELYRRSTLGTSLTDALDTLISDQRIEPQLAMRILANFDRVVSDTLKENVKSKLTFKGHLHTYRFCDDVWTFVIKDVNVKLDQNETINAEKIRIVACNAKKAGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7Z1</accession>
    <name>A0A1E3P7Z1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_78157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040742.1">
        <property type="nucleotide sequence ID" value="XM_019184674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7Z1"/>
    <dbReference type="GeneID" id="30201920"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="282" mass="31435" checksum="B472B4C50B061E1B" modified="2017-01-18" version="1">MPKHSRKPQVGSIKPSTPVVSDAEESMEDQLEHESPSDLEFASPAKTISSSAAQQTSPLKKKKLDFKMTSPAKLSKRSEDKVELEALEGSNVSCATEDEPATAVGETSTTGGSEEEEKRRRKREKRERRERRRLAKAASNNQVPAPETSRDSIVSSKELCGAESIISNDTQMEIVELQEPTDKNGSSLMSPETAATQKHRSTNAEHLIDNEKAYEIILRGQAMREYDPHFVEVEGLKIDGIFTYIGCACCRRGEKTIRSKEHANSCAYRFQQDNCFNKKLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8E0</accession>
    <name>A0A1E3P8E0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FMR1-interacting protein 1 conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_77784</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040344.1">
        <property type="nucleotide sequence ID" value="XM_019184612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8E0"/>
    <dbReference type="STRING" id="683960.A0A1E3P8E0"/>
    <dbReference type="GeneID" id="30201858"/>
    <dbReference type="OrthoDB" id="273070at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019496">
        <property type="entry name" value="NUFIP1_cons_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10453">
        <property type="entry name" value="NUFIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMR1-interacting protein 1 conserved" evidence="2">
        <location>
            <begin position="96"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="211" mass="24393" checksum="7269F4A155CAEEAC" modified="2017-01-18" version="1">MEYIYGLPPPPPSSNNGNAKKDNNNNHMKPGKNQNNRRQQQNNNKQNSRPNPQNQAYLDLPTHLPNEVPKQQPALVHEQGEVIDEVNQTEAAKEVVPVFGTNIVLKTEEDIEEWVKQRKLNWMKKISNSRPEPESKPAPATSNQPPKRTQRTSDPRLNRNQTRVQKPQHKNNHNSNKTNLNNLIIQREIQHENKAILDVIKELFEVQILKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8M1</accession>
    <name>A0A1E3P8M1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-beta hydroxysteroid dehydrogenase/isomerase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_24923</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040771.1">
        <property type="nucleotide sequence ID" value="XM_019181074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8M1"/>
    <dbReference type="STRING" id="683960.A0A1E3P8M1"/>
    <dbReference type="GeneID" id="30198320"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05227">
        <property type="entry name" value="AR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002225">
        <property type="entry name" value="3Beta_OHSteriod_DH/Estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01073">
        <property type="entry name" value="3Beta_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-beta hydroxysteroid dehydrogenase/isomerase" evidence="3">
        <location>
            <begin position="8"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="338" mass="38512" checksum="9A6D91665D9B950A" modified="2017-01-18" version="1">MPSQTVFITGATGFIAQYIIDELLKQENKVIGSVRSIAKGDNLVKLFQNDKFSYVVIKDLIESKQDFDKVLKENPDIEIVLHTASPVTKTEGNVEETYLKPAINGTENVLNSILEFGTNVKRFVYTSSNSAVHSKNRESPKFVETEESWNDVTWDQAIADPSTYGYSASKTFAEKAVWEFVKSKTPTFETSVVNPVFVFGPQLFKESIENQLNFSNDFIYSFFRDGENYKKFQGSFIDVRDVAKAHLLAFQNENTINKRLILKESTFTEQTLLDLIHKEFPILNDKIPKGEPGSDEEILKKRVILDNSKTKEILGFKFKSLKQTIHDTVEQIIEVKGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8M7</accession>
    <name>A0A1E3P8M7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_59839</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040966.1">
        <property type="nucleotide sequence ID" value="XM_019182883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8M7"/>
    <dbReference type="GeneID" id="30200129"/>
    <dbReference type="OrthoDB" id="4069757at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="167" mass="19042" checksum="6F1E25485EA2FE4F" modified="2017-01-18" version="1">MQKTTIIISELSKPELLKVSITIKQYLVDYQKQNNITESQLVENWSELPTLSRLVIIFTEEKYATDIFQHLNTALPNHKVHLSESLLRRHKSFGSLEAPSNLEQAQSSLYQEPEPIKNNEIKLEENQPPRTLFKPDLSLNTEMNPKAPSPRGESPSSPTITLEECST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8N3</accession>
    <name>A0A1E3P8N3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_61868</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. ARD1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040519.1">
        <property type="nucleotide sequence ID" value="XM_019183261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8N3"/>
    <dbReference type="STRING" id="683960.A0A1E3P8N3"/>
    <dbReference type="GeneID" id="30200507"/>
    <dbReference type="OrthoDB" id="25586at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990189">
        <property type="term" value="F:protein N-terminal-serine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990190">
        <property type="term" value="F:protein-N-terminal-glutamate acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045047">
        <property type="entry name" value="Ard1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091:SF4">
        <property type="entry name" value="N-TERMINAL AMINO-ACID N(ALPHA)-ACETYLTRANSFERASE NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="5">
        <location>
            <begin position="3"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="212" mass="24280" checksum="BB5DFA3267E356AA" modified="2017-01-18" version="1">MAITIRLATIADVQGMQNANLHNLPENYQLKYYMYHILSWPEASYVATTTDGPSNGEEDQNSYTKDVKGDPSYVNKNEKIVGYVLAKMEDDPDAEDKTPHGHVTSLSVMRTYRRQGIAEKLMRQALYSLTETFKAEYVSLHVRKSNRAALHLYRDTLQFEVLSIEKSYYADGEDAYSMKKVLKLDELLPSTFQKSEFVDDLETDLLEGIEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8Q1</accession>
    <name>A0A1E3P8Q1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">IMS import disulfide relay-system CHCH-CHCH-like Cx9C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_25266</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040801.1">
        <property type="nucleotide sequence ID" value="XM_019181122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8Q1"/>
    <dbReference type="STRING" id="683960.A0A1E3P8Q1"/>
    <dbReference type="GeneID" id="30198368"/>
    <dbReference type="OrthoDB" id="276296at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2900">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052848">
        <property type="entry name" value="CHCH_domain-containing_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031731">
        <property type="entry name" value="CX9C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47106">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47106:SF1">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16860">
        <property type="entry name" value="CX9C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMS import disulfide relay-system CHCH-CHCH-like Cx9C" evidence="1">
        <location>
            <begin position="10"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="113" mass="12662" checksum="FEDFB96E625214DB" modified="2017-01-18" version="1">MSNILDQLLVEDIARYCPQNFLNFHKCISQNHANPQNCAPQQIELSKCIKSEVPSLQKVMTNCGHLLKEYETCLKTNIAEQGKCLPLLGQVRDCASESIDLSKNTMNNLNPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8Q8</accession>
    <name>A0A1E3P8Q8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GPI transamidase component GAA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_75989</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041002.1">
        <property type="nucleotide sequence ID" value="XM_019184306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8Q8"/>
    <dbReference type="STRING" id="683960.A0A1E3P8Q8"/>
    <dbReference type="GeneID" id="30201552"/>
    <dbReference type="OrthoDB" id="445301at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007246">
        <property type="entry name" value="Gaa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13304">
        <property type="entry name" value="GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13304:SF0">
        <property type="entry name" value="GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04114">
        <property type="entry name" value="Gaa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036762">
        <property type="entry name" value="GAA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="353"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="458"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="538"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="572" mass="64100" checksum="B4721CE92FE42591" modified="2017-01-18" version="1">MALLEKVHKTIVRLGLLPKMIRALPKISLFLTIIGVIWIAVLPMEGQYRGTYISENALMPSQAYSYFRESEWNLLRGYRTQLQDFQRNDTDYESNLKIMQGWLNDIGYKTDIQHSAQGSNLYGIWHAPRGDDTEAIVLGAAYHNSDGIFNLGGLSITLAMARYFHRWGVWSKNIIIVIPQNPNSALRSWVNAYHSTLDLTGGSIESAIMLDYPSASDNFDYIELYYEGINGQLPNLDLVNVAVSVSEHEGPRVSIQGTPQDELDKNDYWSRLRILGRGIVEMALAGVRPGHGNEAFSGWRVQAITLKAKGESGPYDITTFGRIPEALFRSVNNLLEKFHQSFFFYLLLAPRRFVSIGTYLPSAALITASYVVSSLDKVLNSGFETSYLIPYAIPAGGVMSIAIFVGLVISQLIPLLPQLFAYILIGVFMLFSGGSLFYKINLKNELKVILRSLALLYYSTVLSSLLVLNFSLTFAIGVLALPLTFIQDSLSDKRIMLCLLLSNPFIISLFIAQDFENGLSELLYGLTTSWKELNCHTWLVITIGWLPTWLTIAFTTLIKDDSRSETAEKKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8S3</accession>
    <name>A0A1E3P8S3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RTA1 like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_18955</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lipid-translocating exporter (LTE) (TC 9.A.26.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040917.1">
        <property type="nucleotide sequence ID" value="XM_019180811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8S3"/>
    <dbReference type="STRING" id="683960.A0A1E3P8S3"/>
    <dbReference type="GeneID" id="30198057"/>
    <dbReference type="OrthoDB" id="3358017at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        </location>
    </feature>
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        <location>
            <begin position="198"/>
            <end position="215"/>
        </location>
    </feature>
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        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ61710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="23968" checksum="D634C2B7228BB47F" modified="2017-01-18" version="1" fragment="single">FAPMIVGLAFEVVGYICRCLGHYNKESLGPYVIQSVFLLVAPSLIAATIYMIFGRLLILLDAEKSSFIPVRFLTSFFVCGDVLSFLLQAAGGGLMAKADFADTGSKIVVGGLVVQIVFFGFFLISELYFTIRIRSHPTSISQLLRESNERSGVSAKLVNWRMLNISLILASILIMMRCIVRLVEFAQGFTGYIMTHEVFIYVFDGLLMMFACVIVL</sequence>
</entry>
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    <accession>A0A1E3P8U9</accession>
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        <recommendedName>
            <fullName evidence="8">VASt domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="9" type="ORF">WICANDRAFT_61928</name>
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        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
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    </organism>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
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            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="2">Belongs to the YSP2 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="F:sterol transfer activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319:SF36">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1060"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="8">
        <location>
            <begin position="642"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="8">
        <location>
            <begin position="848"/>
            <end position="1017"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="1171"/>
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        </location>
    </feature>
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        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        </location>
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        </location>
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            <end position="625"/>
        </location>
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        </location>
    </feature>
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        <location>
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            <end position="828"/>
        </location>
    </feature>
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        <location>
            <begin position="1018"/>
            <end position="1030"/>
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    </feature>
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        <location>
            <begin position="1180"/>
            <end position="1190"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006582"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ61372.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1190" mass="131161" checksum="12E5B3302AC409F6" modified="2017-01-18" version="1">MPAQYGMNDNINNGYSRPKSPIMPEIMSIKSQRNSTDRSIFRMRSRKDKHSGDNGDSDGQAHSYSDSIKQSKFNSNEPALLSLPLVKSDSMQQDSHSNISQNHSRNSLHEPIAESSPNSRQRSSDIDTPTDVEAHMAATDSTLKNRPSIVNAEANSSGLFSSLVSAAHNAASHIVSSSRQPEEFKSHPPAEHSEAEGTSFMQHLDSLLFSQPKRASREASAESPTKSGADEDDDRASQISKPQSLAHDVVFQPIRKNALSTLGKGELTLEALGFTDASLHSERNSINRGSMNQVPQISIDAPADSKLNQSNSINRSRQLTESNGATTVTNGNGAAITKVVSGPSAFASDSIRGLGDDNNNKGRSSSPVSTFRRSLSPGNLAKMAKSTSAVPTQDTFTNGSAKKLSHRRSVSSGTENQSGINQTASIVSAETNNSTTPPYVPPISPAESLAELKDVNYASSKRNAEFHSMFKKIPKNEKLLEDYSCALQKDILIQGRMYITERHICFNSNILGWVTSIIIPLQEIVQLEKKSIAGLFPNSIVIQTLHQKYVFASFLARDVTFDLITNIWNKLVRGPNGNIDMQVDLDDDFSGSDMESDLDESDDYDSNDEFDTDFDGTSSDGEDEDVLTKHSPTEPEHKEVSGETKIRQGNIEAPLTKVFQTLFGDDTSNYKKIIESQKNRDISSIPKFQKNGDDVAKREYTYIKPLNGPVGPKETSCQVVETLDFKNFDSYVLVTQNTKTPDVPSGNSFSVQTQIYLSWGGNNSTDVTVYTSIQWTGKSWIKSAIEKGTIDGQKESIGILIDELQKMLSSSEGDDLSDSSDSNNVSSLPTVGPATHEPTDNEYKKTNGETQIADEILHAPLGTIYSLLFGDDSTYFKSILETQKNFDLTPIPNFEKDGDLKKRQYSYTKPLNGPIGPKQTRCNITETIEHFDVNKYILVSQVTASPDVPSGNAFTVVTNFYLSWAANNSTRLLVITSINWTGKSWIKGPIEKGTIEGQNESIAVLIHEIETILNKASSGKKGKKKTKSKSRSNTIKEPIKIPSQEMEAKNPLGMIGNLQIPYAFGGLIIVSILLWFIVRFFMVSNNSSSYTSIDHVQKVIIEGREYMIIPSVEQSFKDDLLKEQSEYEIWKWINERTKSDKIDGGEKNHENQDLNEMVRLAEQHLQVLKSQIAHDEEESSSSSSSSSLTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8W4</accession>
    <name>A0A1E3P8W4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_25156</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Tango6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041063.1">
        <property type="nucleotide sequence ID" value="XM_019181108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8W4"/>
    <dbReference type="GeneID" id="30198354"/>
    <dbReference type="OrthoDB" id="39591at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057407">
        <property type="entry name" value="HEAT_TANGO6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019451">
        <property type="entry name" value="Rtp1_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039600">
        <property type="entry name" value="TANGO6/Rtp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20959">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 6 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20959:SF1">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23565">
        <property type="entry name" value="ARM_TANGO6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10363">
        <property type="entry name" value="RTP1_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TANGO6 HEAT repeat" evidence="4">
        <location>
            <begin position="247"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase II assembly factor Rtp1 C-terminal" evidence="3">
        <location>
            <begin position="664"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="561"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="568"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="921" mass="105812" checksum="E25D368AD9C37EAD" modified="2017-01-18" version="1">MSERKGLNVPQLSRETALDKIFRELSEFITIDDKEPKDAIAQLYAKLDGDSNNRVDQINAVLAKSFEFVSKIQDYSLRKEAQNKASGDPEKVNLITISLHDMKYFNELMNLIIVQGIYPCLPTGVGIPLEQRRLKSFNIRSKLFRFEKVHNGLETLQKIVKYFEVVFDKGGDVKDLLLKGSGLTDILTALTVLVSVDPSFKSAFQKFENKSDTYNLFNIYTILLQTTRIPRYQAFLSERLTSITVHRENGVMSLIDFVVGIRDDEDVNIDKFNNVNRILIAKPKDMTSVEYFTKLFNQIYEILVFINRPVMSSVVVNFIRVLYSKNKRIIHDFLFKKIWNILSPDTSNFKQGETVVEAKELNDAINVLISLTKETSPEFLNEFFSKIILNLWAYMFYLKKKKLEYYSIISNIMVSFFTITSNNDFLENTLLNLIKSGGDDWKFETNLENKLSSIVKSSILDKNLSSEEIFDDLDIGLEIIVELFKNLNNDIVRTQFLTVLNRWISKQNNDNLLDQENPFIILIDLKFLEKINDQFRDALLNKPEDVLLVVRNLLTTRSTQNSLKKEETNEEVDSDDEDEDDEDIANPGNLNILLELLSAIISETNPIELTRHKHTLKEISQILIKFSSDKHCQALHKRIEDFLSDEKHTVNNGDDEKTKDKELLEKAITSMNDPLIPIRAHGLYLLRQLIVKRSPVISLDFVIELHIVQLHDSEPFIYMNVIKSLSELIEFDKDATVDFLCDFYTKKEEKLDDKLKIGEVILNFISKTGELFTGPLADKLMAKTLGVIQNFEEDNRVRMSAMSLIGQALRTNAIGLEKFIKDALDCSIGILELEKEVIMKRSAVVLISDLISFGGLDIVPRGYGEKLKTVLSYVKVNADDYLLVEQIDKVLEIIDELIRDRLKPSVNTQFDALKIKEISRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P948</accession>
    <name>A0A1E3P948_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Polyprenal reductase</fullName>
            <ecNumber evidence="5">1.3.1.94</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_49021</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Plays a key role in early steps of protein N-linked glycosylation by being involved in the conversion of polyprenol into dolichol. Acts as a polyprenal reductase that mediates the reduction of polyprenal into dolichal in a NADP-dependent mechanism. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a di-trans,poly-cis-dolichal + NADP(+) = a di-trans,poly-cis-polyprenal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80727"/>
            <dbReference type="Rhea" id="RHEA-COMP:19536"/>
            <dbReference type="Rhea" id="RHEA-COMP:19537"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:231623"/>
            <dbReference type="ChEBI" id="CHEBI:231637"/>
            <dbReference type="EC" id="1.3.1.94"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="5">
            <dbReference type="Rhea" id="RHEA:80729"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the steroid 5-alpha reductase family. Polyprenal reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.94" evidence="5"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041105.1">
        <property type="nucleotide sequence ID" value="XM_019182624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P948"/>
    <dbReference type="STRING" id="683960.A0A1E3P948"/>
    <dbReference type="GeneID" id="30199870"/>
    <dbReference type="OrthoDB" id="541710at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003865">
        <property type="term" value="F:3-oxo-5-alpha-steroid 4-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160198">
        <property type="term" value="F:polyprenal reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102389">
        <property type="term" value="F:polyprenol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043048">
        <property type="term" value="P:dolichyl monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016095">
        <property type="term" value="P:polyprenol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039698">
        <property type="entry name" value="Dfg10/SRD5A3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14624">
        <property type="entry name" value="DFG10 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14624:SF0">
        <property type="entry name" value="POLYPRENOL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="6">
        <location>
            <begin position="159"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="266" mass="31181" checksum="7B339704E5E3DFCB" modified="2017-01-18" version="1">MILKHVDIILQIFYAGAIISVLLAKFYPKLNRLLKYGKTLDGSTNKPIEKDNRLLDNLLQIYVPKYWFFHFYVVSLTLSAIFFALLHWGLKSKDAWSYFLILVHSFRRLVENLFILRHSSKSKMHLSHYLVGIFFYSAINLNIIINTSLGEDDLQEEPELDYKIFIPLFLFLIGFGDQFLNHFHLSKTIKYNLPSFGMFKLICCAHYFDEILIYTSVYLLLGNKTSLISLIWVIVNLTVSSIETRNYYKINNEGKIPKWSIIPFVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P984</accession>
    <name>A0A1E3P984_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Brl1/Brr6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_29292</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040644.1">
        <property type="nucleotide sequence ID" value="XM_019181592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P984"/>
    <dbReference type="GeneID" id="30198838"/>
    <dbReference type="OrthoDB" id="5961at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Brl1/Brr6"/>
    </dbReference>
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        <property type="entry name" value="Brl1/Brr6_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136:SF1">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10104">
        <property type="entry name" value="Brr6_like_C_C"/>
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    </dbReference>
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        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Brl1/Brr6" evidence="3">
        <location>
            <begin position="47"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="247" mass="27584" checksum="ACE6DC4588F2D21C" modified="2017-01-18" version="1">MHHHHYYPSNQSTPTSSSQYSPGNEVIPLPAPWSKFASPKAPTPYLISSYLQLLFNVLTSSIAIYILINAIKTVKSDINLKMEEYAADIAMEVSRCARSYVENRCAPETRAPALEVTCSELERCMNRDPTTHAGKASVSAETLGMILNSLIEPIGVKAIVVFGVGLISWAFTSNFIFGFVRAKSYYGWDSNRQDPQQQQQYQQIGWNQQSQQQFNPSTPALLPPGPPQPYMTPGAPQDWQYNYSPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P991</accession>
    <name>A0A1E3P991_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Programmed cell death protein 2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_52607</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ61447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019182694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P991"/>
    <dbReference type="STRING" id="683960.A0A1E3P991"/>
    <dbReference type="GeneID" id="30199940"/>
    <dbReference type="OrthoDB" id="443682at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140597">
        <property type="term" value="F:protein carrier chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007320">
        <property type="entry name" value="PDCD2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47524">
        <property type="entry name" value="20S RRNA ACCUMULATION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47524:SF1">
        <property type="entry name" value="20S RRNA ACCUMULATION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDCD2_C"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Programmed cell death protein 2 C-terminal" evidence="3">
        <location>
            <begin position="282"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="152"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="118"/>
            <end position="145"/>
        </location>
    </feature>
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        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="181"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="403" mass="45056" checksum="6DDD6CBC6DE575C2" modified="2017-01-18" version="1">MSSSGYSSDEEEPEIPKTSGVYLGFIDDDFENAGEEPTIEDSFVGGQPVWLHPNSKPSDDMLSCKNCAGPLSLILQAFSPLEGALYDRVIYVLACKKPSCSRKNGSVRVIRGISKDPKKIAEIEKEQQTELKNQLDEKLKLDEARNINLFEGDASANPFGGSGNPFGGSGNPFDYNPFESQNKEEKPKKVEDTKPNFASVAAKAAPPKQTKQKSSNIKKLPEWPGYFIFVDQEKFKKKNLPKLPEHLKISESALDTEAEVSSSSTTKDLSPEAQHLSNNLQDSVFQHFSDIVSYNPLQVLRYELGGKPLLFSSKDDVAAKVAKNLVPNPGFNPSSSRHFELQIMPKVIMDFERDDIDIVNGINWGTIFVYTDVEDYIPELDENYVGYVEEWAGVQWEEEVQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9A7</accession>
    <name>A0A1E3P9A7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L-serine ammonia-lyase</fullName>
            <ecNumber evidence="5">4.3.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_98602</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-serine = pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:19169"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="EC" id="4.3.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.17" evidence="5"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041202.1">
        <property type="nucleotide sequence ID" value="XM_019186661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9A7"/>
    <dbReference type="STRING" id="683960.A0A1E3P9A7"/>
    <dbReference type="GeneID" id="30203907"/>
    <dbReference type="OrthoDB" id="7773036at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000040">
        <property type="entry name" value="L-serine dehydratase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF2">
        <property type="entry name" value="CATABOLIC L-SERINE_THREONINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="11">
        <location>
            <begin position="11"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ61995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="342" mass="37419" checksum="1C93E1D992E683AA" modified="2017-01-18" version="1">MTIAKHTEVPYIKTPLVDSKELTKEAPCRIFLKQEFIQPSGSYKIRGLSNLIKTSLDEIKANPANNGKKIHVYAASGGNAGNAVSCASNFYNLESTVVIPKATSLKMRQKIERNGSTVLVKGENIGEAAEYLRDVLIPSLGEDVVPIYCHPYDIPAIWHGHSSMVDEIVDQLAARNELDKLKGVVCSMGGGGLYNGLVQGLRRNNLSNVPILTLETDSCPTFEESIKANKQVVIKSCKTIATSLACPYVSSKTLEYYNTHKTKNLLVTDSDAANSCIRFANDLNIIVEPACGVALCSVYNKLIEKNIDFFDDLKPNDIIVVIVCGGSATTVQDLNEYKQLYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9P8</accession>
    <name>A0A1E3P9P8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_17796</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ61607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019180760.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9P8"/>
    <dbReference type="STRING" id="683960.A0A1E3P9P8"/>
    <dbReference type="GeneID" id="30198006"/>
    <dbReference type="OrthoDB" id="4726at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12306">
        <property type="entry name" value="RRM_II_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF12">
        <property type="entry name" value="EUKARYOTIC INITIATION FACTOR 4B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="29"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="137" mass="15357" checksum="26E3B2C1BEE474AE" modified="2017-01-18" version="1" fragment="single">EAAKLRELHADLAKQESETQEEKSDVDARSVYVGNVDYDSSPEELQKHFQSVGVINRVTILLNKFTGQPKGFAYIEFAEPASVVSSLVLNDSVFRGRNLKVVPKRTNVPGFNRGRGRGRGRGGFRGRFRGRGRFNPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9T0</accession>
    <name>A0A1E3P9T0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">INO80 complex subunit B-like conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_66295</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041171.1">
        <property type="nucleotide sequence ID" value="XM_019184036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9T0"/>
    <dbReference type="STRING" id="683960.A0A1E3P9T0"/>
    <dbReference type="GeneID" id="30201282"/>
    <dbReference type="OrthoDB" id="2021186at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029523">
        <property type="entry name" value="INO80B/Ies2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006880">
        <property type="entry name" value="INO80B_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21561">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21561:SF12">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04795">
        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01406">
        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INO80 complex subunit B-like conserved region" evidence="3">
        <location>
            <begin position="280"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="230"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="276"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="239"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="366" mass="41781" checksum="038096424D392463" modified="2017-01-18" version="1">MVSVESDAEINEGKSVAQVKTAGNEEGFDDSIDDSVDEDDEEDEDNDNEDQGEEEDDDDEVDVEEDDGVGADEDELDEDDEEDEFASDAQEDDDDQALSDDDDDANFKINDVDDDDVDEPEDELQTPVPQKKRSLSLKIKLPTSKRERNPIKDDLDDDFDDEIEVKKPKKARKITQTKPDPPVSKRGVGRPKRVATNDVNYDDEDDIVSDSENVIPTPALDDELLLTDEETEYTPDVTKMTERQRAKLENEETGVTQLQDQQLLSLSNDVHKRRVLTDEENQLRKAEIARKRKNLTERKLEEEKQETINKLLKRRAGKVSAATLKEDEEVNLKEKPRRPQIQHEALFTWVSKKDTLALRVPEPLMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9T2</accession>
    <name>A0A1E3P9T2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_14521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041335.1">
        <property type="nucleotide sequence ID" value="XM_019180610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9T2"/>
    <dbReference type="STRING" id="683960.A0A1E3P9T2"/>
    <dbReference type="GeneID" id="30197856"/>
    <dbReference type="OrthoDB" id="94039at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050228">
        <property type="entry name" value="Carboxylesterase_BioH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43194">
        <property type="entry name" value="HYDROLASE ALPHA/BETA FOLD FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43194:SF2">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN LPX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="52"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="315" mass="36033" checksum="CF81D6DB4688B5E8" modified="2017-01-18" version="1" fragment="single">FDRFERIFDAQFPRFNKNATIPPNSNHLKIVVDHYKLRSSLKSPERKPINLYLLHGTGMTKAIWRYYAKRLYEFSETESPIWQIDNIIAMDLVTHGESAMFNMGKLGYEYDWRDGARDLVKIASELNLQGDNIAIGHSMGGFQSVYAAFLAPTLFKFVVPIEAVLNQSENSRKRFKGLLPALNKVITTEFRNEVEYETFMKKKSFYTGFNKEILDDFIKAEKWVHPDGTVSTKTSKQQQMLGYYVGGFITPIALEVVHNIFIPIVHVIGSEATWNEKESVELIRNGHINVLDAVDIKGGQHLVNGEQPDEVIKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9Y1</accession>
    <name>A0A1E3P9Y1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anaphase-promoting complex subunit 4 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_20004</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041317.1">
        <property type="nucleotide sequence ID" value="XM_019180853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9Y1"/>
    <dbReference type="STRING" id="683960.A0A1E3P9Y1"/>
    <dbReference type="GeneID" id="30198099"/>
    <dbReference type="OrthoDB" id="308449at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051959">
        <property type="entry name" value="PAK1-Kinase_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44675:SF1">
        <property type="entry name" value="P21-ACTIVATED PROTEIN KINASE-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44675">
        <property type="entry name" value="PAK1 INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="65"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="119"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="161"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="298"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="369"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="370"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="404" mass="45559" checksum="39788997788B28E6" modified="2017-01-18" version="1" fragment="single">KDPKSSSKPSVIKPTIIPPTSSTTSTSQAKTQFRIYVGSYEHNLLSLSLILEKEKTPVFQPIFHFQAHALSIKSIAAAKRYLVTGSNDEHIKIYDLQKRKELGTLLGHQGSITSLRFSHEGHTDGEEVLGSGASGKWLLSGSDDGKIIIWRTKDWEIFGTLKGHKGRINDISIHPSGRIAVSVGEDKSVRLWNLMTAKKAANLKIKNDWQSGEFVRWSLTGDYFFVGLLNKILVYKTSEAQIIKEVPFKSTLMHIEVYKIDDVEYAVVGLNNGSLEFYDVEDLTKVEDDKEPEPKFKLVGHTNRIKDFKLYRVNNETYLISISSDGNIVIWDLALKDQVAVYNTGERLNCVEVSSESVEKFETMKRRIDDIDDEEAASASESENEADQAELKRIMEGRKKKKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9Y5</accession>
    <name>A0A1E3P9Y5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_87397</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041385.1">
        <property type="nucleotide sequence ID" value="XM_019186108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9Y5"/>
    <dbReference type="STRING" id="683960.A0A1E3P9Y5"/>
    <dbReference type="GeneID" id="30203354"/>
    <dbReference type="OrthoDB" id="289314at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06867">
        <property type="entry name" value="PX_SNX41_42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044106">
        <property type="entry name" value="PX_Snx41/Atg20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051079">
        <property type="entry name" value="Sorting_Nexin_Autophagy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015404">
        <property type="entry name" value="Vps5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46979">
        <property type="entry name" value="SORTING NEXIN-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46979:SF2">
        <property type="entry name" value="SORTING NEXIN-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09325">
        <property type="entry name" value="Vps5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="11">
        <location>
            <begin position="103"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="427"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="488"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="67"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="427"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="563" mass="63305" checksum="BBE746BAD1435BF0" modified="2017-01-18" version="1">MSIQEEDNSPCAQSTVLQEDEFIDLPLSQDEDEDSQEPTHNEEELQQDNEPESDTNDVPPDSPTTLSTASQPQSAPAVASNTTTYDPEVYTSTTQLAFKDKKSLKIEIIDAGKSREGHSRGYIVYTIKCNDSLVRRRYSEFESLRNTLVKLFPTLIIPPIPEKHKISKYAAAPTKAKEDTRIIDHRRRMLSVFLKRLLEIDQVRESQVYQSFLDPNANWPEVLTSPPISDIPKNILQADPLDPAHSTAAHSYLPLPSSTVIVVKETQEDKKFNEVEANAKEYEAVISNGIDKANKRIVKHLSDMSTEFSEAGAGFNSFSLQENGKLASTLEKVGQVHDNCYLSTETLVNSLNYEFTEPLGESVQFASVVKDIIKFRQQKALQLDITARTLKHKSNHLNLLQKSEQESKRIDQALDAESGKTQQINFDRKQAAKDAQAKANQDAGLNQSVTPLEPKKSSKFKIPGISKISNVIKEAIESDPEVTRQHNLTKTKDDINQLKQTVTAAEEDLGKVTKSIKNELERFQISKEEDLKKMILAYSACVLDWSKKNLEFWEEAKADLQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9Z8</accession>
    <name>A0A1E3P9Z8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dipeptidyl aminopeptidase A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_59851</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S9B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040979.1">
        <property type="nucleotide sequence ID" value="XM_019182885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9Z8"/>
    <dbReference type="STRING" id="683960.A0A1E3P9Z8"/>
    <dbReference type="GeneID" id="30200131"/>
    <dbReference type="OrthoDB" id="16520at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF160">
        <property type="entry name" value="DIPEPTIDYL AMINOPEPTIDASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="10">
        <location>
            <begin position="254"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="9">
        <location>
            <begin position="699"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="40"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ61772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="901" mass="102662" checksum="7FCC4930F263AF93" modified="2017-01-18" version="1">MNGFFDRFKLGSQESYEMVDGSQLNDVDLERGVEAEAGQSDNRVSSESTRSSSSIDSQTSELLLSINKYSASNNGVEDFTSNPKFLAAKSGGIRRKFCLFIIIIGICLWALLLLAYSLGAERHYDYAKNFHKGNKTTNGSTIISSSTISSAVIRPTKSIIPLVEYDIHTSLEPIPTKSLGTDLLMDDVQFLQAGATKLSKKEDEGYYVLKQNNDFVIKKLSDRAYSQILMKDTTFKFREQEHIITSVTPNYNVTYGIVQSDFESVFRHSGKSKFWLYDFNAGEFTPIVGDLEDEKLSLAAWSPNFNYVTFVQANNLYIYDVREKKSTKITNDGSVDILNGRTDWVYEEEVFATDHAFWWAPDDSNLIYMRTDDSKVHTQDLEYFISNDQYPKVDKIKYPKPGSANPIVSLYQYNIKAGEVSKIDRAGSSLGDDFIVYNCQWIDETNFVIREADRESNILNYRVYNPEDKSSKVTYTVDAVKEYGGWIDKFGEIFPVPRNSSAGRETPGFIDIIVVDGYNHLAYFDSADSLNPIPLTSGQWEVTSDVLSFDSEENLIYFTANRQSNFENHLYSLNLQTKQLIAMSEIDEIGYFYASFSPSSRFSVLTKNGPSFPLMYAQNSLRKRGVLSFTGENKSFHKVQVDEDSDGNPVTVNVIEVLPKHFDENGKHPLLVNVYGGPGSQKVTSKFSVGFEDSISERFNAVILFIDPRGTGGQGWRYRSWSRGHIGHWEPRDITSLTKKWIDSRTYIDEEKTAIWGWSYGGFTTLKTLEFDQGNVFKYGMSVAPVTDWSLYDSIYTERFMDKPENNKQGYGESRINDVDGFRTVKRFLVMHGTGDDNVHIQNSLRLFDKFNLVDVRNYDMQLFPDSNHNINSHNSHIIIYKKLETWLINAFEGHFDEIAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAB0</accession>
    <name>A0A1E3PAB0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_82372</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041515.1">
        <property type="nucleotide sequence ID" value="XM_019185370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAB0"/>
    <dbReference type="GeneID" id="30202616"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="76" mass="9011" checksum="BE908A92871DFEA2" modified="2017-01-18" version="1">MNRLGTLNWSTQIEESQDHTNGLRGRENWSESLLFQEVSEHLKAFWVEESFCWLDAGEPVKYLAKQLPKNNRKFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAK9</accession>
    <name>A0A1E3PAK9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA ligase</fullName>
            <ecNumber evidence="15">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_27446</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="15"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041666.1">
        <property type="nucleotide sequence ID" value="XM_019181373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAK9"/>
    <dbReference type="STRING" id="683960.A0A1E3PAK9"/>
    <dbReference type="GeneID" id="30198619"/>
    <dbReference type="OrthoDB" id="151490at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032807">
        <property type="term" value="C:DNA ligase IV complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097680">
        <property type="term" value="P:double-strand break repair via classical nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043007">
        <property type="term" value="P:maintenance of rDNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07903">
        <property type="entry name" value="Adenylation_DNA_ligase_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07968">
        <property type="entry name" value="OBF_DNA_ligase_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044125">
        <property type="entry name" value="Adenylation_DNA_ligase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029710">
        <property type="entry name" value="LIG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45997">
        <property type="entry name" value="DNA LIGASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45997:SF1">
        <property type="entry name" value="DNA LIGASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 15" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="17">
        <location>
            <begin position="391"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="18">
        <location>
            <begin position="682"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="18">
        <location>
            <begin position="831"/>
            <end position="940"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ62459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="945" mass="109809" checksum="BD975559412EC468" modified="2017-01-18" version="1">MNQEGVKEELPKPWELTKYVDTDLEDEDAPKNFGGPLPSFKWLCEGLFDKLDKAFQKSNYYTNGRQTVTEQKQRIVDLFFQEWRSTVGPNIYPALCLILPHRDRSRIYNIKDYTLGRRILEVLKIPKNSDSTTVIMNWKRSRNAKGARMSEICIDVIKARRTDNKGGKISIGELNQILDKLARFDSKREEQIEILSYLIENMSFLELQYFFDILLKKNVVRNMENRILNSWHPDAQQYLSVVTSLKTVAYKLYDPSKRLNEHDLSINIGKPFAPQLSLKPHLTYAKVVQKLNNDFFIEEKMDGERIQIHYEDYGKVVHYWSRKATDYTYLYGESLEKGCISPHLKFVKAVKNCVLDGEMITYDPQRNAILPFGVLKGSAVTELQRLDKGEIEELSARPLMMVFDLLYLNGRSLARVPLEERKKWLSQILSPTPKFVEIIQVSKADNEEAIKRALTQAIERGSEGVVLKQTRSLYQIDKRTDQWVKIKPEYLEEFGENVDLVVIGRERSKKDMYFCGLRITDDDNEDEKSPKFHSFCRVANGFDKNEYREIERITKGKWKDYSKVKPPTSLLEFGKREPQEWIDPRESFVIEVKARSIDRSISKNYKTSTTLYNAYNRKIRDDKDWTTAATLDEYKAIKESRTSVNTTEQKLVTKKKRLIRKRKRESQDEEVWNDQDYLKISKNSNIFGDLTFMILSDAFYKNVRISINDLTELVHKNGGKITKNETKVPDLHNLRVISDKFTIQADALSSKGFDVLKSSWFFKCIELGVILRVEPKYCFKVSENLKSKVETRVDSLGDSFSVPVTEDELIKIFEDNKNVVHEKSEIDEQLMEIPLFGGLKVYVSELEDSSTHYLYQQAITKLKLFGAKITDELEDCNLVVIPEYIEHELVSNVAKDIRKKLSSLLKYTESSVTIARIVNDKWVSASIQQKTQVDAKDYPVIASRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAQ0</accession>
    <name>A0A1E3PAQ0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_13427</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EBP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041678.1">
        <property type="nucleotide sequence ID" value="XM_019180573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAQ0"/>
    <dbReference type="STRING" id="683960.A0A1E3PAQ0"/>
    <dbReference type="GeneID" id="30197819"/>
    <dbReference type="OrthoDB" id="443772at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000280">
        <property type="term" value="P:nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008610">
        <property type="entry name" value="Ebp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028">
        <property type="entry name" value="RRNA PROCESSING PROTEIN EBNA1-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028:SF0">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EBP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05890">
        <property type="entry name" value="Ebp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="295"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="227"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="29"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="71"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="305"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="366" mass="42370" checksum="7BFD44A64CDAA358" modified="2017-01-18" version="1" fragment="single">KKDEEYKSDVLSKKEQRKLQKAISKAQGEEDEDEEEESGSEEDDIEIAEDEDSEMEEYLLDLEKLARSDSESESEDEEDEEEEAQENEAEEEDDEEDDEEDDEEEEDIPLSDVEIDSDADIVPHTKLTINNVGALKNALTRIQLPWEKYSFQEHQSITSAQAVEPEIKDIYDDTQRELAFYKQALNAALQGRTKLLKLKVPFTRPQDYFAEMVKSDEHMDKLKFKLIKEASEKKASAEAKRQRELKKFGKQVQNATLQDRQKQKRETLDKIKGLKRKRQNEEISANDFDVAVEEATADIDERKDRNKRTKPNAKRMAKDSKYGQGGMKRFKRKNDAESSADISGFSQRKMKGKSAKRPGKSKRNNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAQ2</accession>
    <name>A0A1E3PAQ2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_60555</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041706.1">
        <property type="nucleotide sequence ID" value="XM_019183015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAQ2"/>
    <dbReference type="STRING" id="683960.A0A1E3PAQ2"/>
    <dbReference type="GeneID" id="30200261"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025928">
        <property type="entry name" value="Flocculin_t3_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13928">
        <property type="entry name" value="Flocculin_t3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133761" description="Peptidase A1 domain-containing protein" evidence="5">
        <location>
            <begin position="20"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="6">
        <location>
            <begin position="52"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="481"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="760"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="916"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1010"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="761"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="771"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1028"/>
            <end position="1045"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1068" mass="110358" checksum="B2C53436ACEDB7C4" modified="2017-01-18" version="1">MLSYKCLLGIASLLPQAFALLRVPVGPNGGLERLVKKDAFETDTSVQTYIIPGTDFYFGSEHQKVFVSFDTGSYTSYVQDSSISKNGFNGNDSTSLISLHKDYSVDYADGTSFKGTWVADNVSFDNENTHTANLTFGLVDDAADSTNIFGLGYYPDSPDPTFIDTLFKQEIISKRVFSVYQDSLTKGEFTFGGIDTSKFSGDLTAIPVIDAQSWNVKLFDFYHGCETLTNGNSYPILIDTGSSTGLYLPEDVYNKLINKLEAKYESTLKGYIFDCLKAQSLTLDFGGIQIEIPTSSFVQGVSGTDYCTVNWRVSSQGSFQLGWTFFNNFYFVFDAENKEILIGEPIKNSSDESVVEITGDVPSSVQAASYSQTDIVSTNSIDYTSASTVSIFASSSSLGDYYNGPTGLTTCTPTSSSSLSSSSSSSSASSISSAISSSSVISSSEAISSSESSSSLSVFSTSASSSDSSIESSSSAITSSINESSSSDASSAVESSVSSAEPSSSGITSSINELSSSAVESSSPVLSSVASIESSDVSNAASVSEYPSSFPSSFPTISATVPSSSSLLSSSPSALSSTFSGYFNSSSSVPVVSESSSALPSSSATPSINGSFVDGSPIWDVYIPFELGPWSLVEIYSAKEAEFTYSHIDLLVSGSKVSPSVFQQNGGSFQYNYDGEIEDGETLQAHIVAAPFTGQSATIDIVIAITDRNGAKLVKRATQSFTLSNTITASASSSLSSTSSSATTSVLSSLIASESPSASATVISSPSNSDTASTVEPVSSSSISEASISGTGDSSNVVAGELSTSTAVVENLQTTIATVTSCSGHVCTEEPTTVVVQTVTQTLGNIINVYTTYCPLSSDSSYSPSSVPNTVASTSETTPIAVPSTTTIKEESSATVASTSETTPVAATSVTTVKSSAEVTPGKSSISTSKASVSIGSGVESTSTQVVQVTSTLPDGKVTTIASTQESSAPASSGTPVSNGSALVSTQLVPTSTQGDSTLYVTHLVTQTTEPVSGGVSSSSIPKLLTQGGASNNGTNPQSPAQVSQYEGSGNRLVAGMLSALPIVVMFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB06</accession>
    <name>A0A1E3PB06_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">THO complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_16167</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the THOC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041621.1">
        <property type="nucleotide sequence ID" value="XM_019180679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB06"/>
    <dbReference type="STRING" id="683960.A0A1E3PB06"/>
    <dbReference type="GeneID" id="30197925"/>
    <dbReference type="OrthoDB" id="29024at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040007">
        <property type="entry name" value="Tho2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021418">
        <property type="entry name" value="THO_THOC2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021726">
        <property type="entry name" value="THO_THOC2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032302">
        <property type="entry name" value="THOC2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21597:SF0">
        <property type="entry name" value="THO COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21597">
        <property type="entry name" value="THO2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11262">
        <property type="entry name" value="Tho2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11732">
        <property type="entry name" value="Thoc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16134">
        <property type="entry name" value="THOC2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THO complex subunit 2 N-terminal" evidence="8">
        <location>
            <begin position="14"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunitTHOC2 N-terminal" evidence="7">
        <location>
            <begin position="498"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunitTHOC2 C-terminal" evidence="6">
        <location>
            <begin position="772"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunitTHOC2 C-terminal" evidence="6">
        <location>
            <begin position="970"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="200"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1084"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1084"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1105"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1131" mass="131402" checksum="81A7AFFBB121F991" modified="2017-01-18" version="1" fragment="single">QKNKTDHILGNYVEQKKYNLLMESSEGFAKVALLLFEAIDSEYSAFQIDYTVNAIDRLIGHFSLDPIRVLDVILDIFTNTILSNYRFCIDFLRKSQWWPKNNESDNSSLETLNKGGNKEAALLLGIKLKQYHDALSKDVPETFKILISILIKEGFISFGDVVKYLAPDDKVMNALEEQIKKNIDDEIFKASASALALSGPLADDEDDSAKKDGSKAKSTLSNNSKEDTEQKLKTLNQKLQMTKLFLTVGLYWPSIYMLTKYPYLVHADSDFIPLILKLFNVMVEPLKKKVCPLNAEETKELRTEKPTPFSRRTTANDAVEFEEYIYNHVELLRPLHHDHTNRKFHFFYHEWSTGLPSITTTEELFKCSAELLSFVGAKIGETPELITLLSRIGVDDLNTAEGSENYEEKLNLWFEYFRKYIFPASTALTENSITTYDIFELLKKFPLQKRYNLYGDMVNNISKNNNIVRLNYSKAEKQTKDVLKRITTENVKPMMRRFAKIAFANPIPSFLVIVTQVESYDNLSNLMVEAARYFTDYAWDVLPFVLLMRLTARRNFIQSDGMNEAQWLQSLATFIAKLGKNYAPMNLTPILTFIIKDLHKSNTIGLTILREIVNQMGGIQQSSNLSANQIKLLNSGESLQRSINKVIFDTREECVKPALRLLSHLINSNQLSELFILLCRFHSELIERSDDFAHYKILSNQSDEITSVLHTYIELINYFLRNDTELFKKNVIDLSELVTKYGVEVSWAFELWRKNVSLEIADQIEYADVDFETLNKDLYTSFWKLSLYDINYDKSLYDHEEQRLKDANTSIVDHLERLRKERFTLEVETKELKLKKEKRFNDEVLAKIPEDDEAHKTHSEEILKNIEERLGNWVNEENPSTEEIQAFYQSCVVPRAIHSETDAIFTGKFITSLLKVDVSQKFVDLFLTSEVLDTLIYTITPLEAENLGFFLAEIFRFYEDVRNSEGLSNEVKEVIYKWHTNALSSIKLNIESENYMSRRNAFTLLKNLIDVYPIVEDHAEQIVESIEKVSNSEERKDLKLASDAILAHIKSRSKKWIHLWDFYYFPEEEKEKVIEKRNLLKKEEKNEEKESVTIKKDEEEEEEEGVKAKKEIELNEKKEEKESIKEEEAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB25</accession>
    <name>A0A1E3PB25_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_50290</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041826.1">
        <property type="nucleotide sequence ID" value="XM_019182648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB25"/>
    <dbReference type="STRING" id="683960.A0A1E3PB25"/>
    <dbReference type="GeneID" id="30199894"/>
    <dbReference type="OrthoDB" id="201153at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13970">
        <property type="entry name" value="ABC1_ADCK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034646">
        <property type="entry name" value="ADCK3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051409">
        <property type="entry name" value="Atypical_kinase_ADCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851:SF3">
        <property type="entry name" value="COENZYME Q8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="5">
        <location>
            <begin position="221"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="560" mass="63610" checksum="4DA1BA642ACE1EB0" modified="2017-01-18" version="1">MLTREGVHEILTISKAAANVARASLDLVQTEACNFYKSSILTKNCRIHDEMYDELNSKSQSIRTGTTHSTIIQDDKRPKINPQTPSTSRNYSTKAVKPTIIPEITEKTRQPETRDPPAKVAAKILDESRYAKIPSTKLQRLFHYGHLAANIGLNTATDGLRAYASGEKTDLRKLALSSKNVSILGDKLRKMRGAALKVGQMMSIQDESFLPREISDLLKNVQNQGYFMPPSQLDRLMKLNLGEDWNTKHFQSFDEVPIASASISQVHKAVLKNGSPVAVKIQYPGVKDSIDSDMDNLLMFMTASRLLPRGMFPEKSIQNARTELKWECDFNREAENIKKFAELLGKDSVFQVPKVYDEVSNENILTMDYLKGVEVCRGSWDQSTNDWIASNILRLCLQEIAQFKFMQTDPNWANFLFNEDNKKIELLDFGASRDYNDEFIKNYVSCLRAAVDKDRELVKHYSQKLGYLTGLETEEMADAHVDSVVILGEPFCSDGLYDFSKQDVTTRVKAKMGIMLKERLTPPPEETYSLHRKFSGVFLLCTRLKAKVPCKELFDEYFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB27</accession>
    <name>A0A1E3PB27_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arf-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_82191</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041284.1">
        <property type="nucleotide sequence ID" value="XM_019185346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB27"/>
    <dbReference type="STRING" id="683960.A0A1E3PB27"/>
    <dbReference type="GeneID" id="30202592"/>
    <dbReference type="OrthoDB" id="10266696at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010512">
        <property type="term" value="P:negative regulation of phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08204">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051718">
        <property type="entry name" value="ARF_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705:SF9">
        <property type="entry name" value="PROTEIN GTS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="3">
        <location>
            <begin position="10"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="128"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="221"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="337"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="258"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="393"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="419" mass="47451" checksum="34B049E5575A8835" modified="2017-01-18" version="1">MSKRNQKRIEHELKDLINDRSNANKCGECGAGFPTWASVNLGVFLCGRCASLHRKLGEDVSFVKSLSLDKWDEEDLDSLSRIGNKKAKQIWNPKKVPFPFDDDDKDEIYQYLRQKYIQGKFRYDPIDEDDYNLRPKSRSHKSRSRSSTVNSRPSSRPSVSSRSIPRLSHRDISEYDRLKLRSLEKRLEEQGFTNTDDNYESLYLAKGDFNLAIDILSNSAADDRPEAKPALPRRPGTSVSNGGGSSQSISQPVSASSDWWNGASQQTPSPAIFDGTTGVNPQQATGLIQPPQQYLDPATGIIYVDPIQQEQYLQQQQQLQLQQQQTGFFQQTSQQQPQQTGVFQQPQQTGIFQQPQQQQQTGAFYQQPFQTGGVMPQRTGVDKNQLLSLYNTGGQPQQQSQQQQQGYQFNNGFNGYSYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB55</accession>
    <name>A0A1E3PB55_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_99052</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL31 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041853.1">
        <property type="nucleotide sequence ID" value="XM_019186678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB55"/>
    <dbReference type="STRING" id="683960.A0A1E3PB55"/>
    <dbReference type="GeneID" id="30203924"/>
    <dbReference type="OrthoDB" id="9739313at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00463">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.440.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00410">
        <property type="entry name" value="Ribosomal_eL31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000054">
        <property type="entry name" value="Ribosomal_eL31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020052">
        <property type="entry name" value="Ribosomal_eL31_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023621">
        <property type="entry name" value="Ribosomal_eL31_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956:SF0">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01198">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01380">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54575">
        <property type="entry name" value="Ribosomal protein L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01144">
        <property type="entry name" value="RIBOSOMAL_L31E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13702" checksum="56E39D80F84149F4" modified="2017-01-18" version="1">MSSNRSALKDVVTREYTINLHKRLHGVSFKKRAPKAVKEIKKFAQKQLGTNDVRLDPRLNQEIWKRGIKGVPFRLRIRISRKRNEEEDAEEKLFSYVEPVVVASAKGLQTVVVEEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB68</accession>
    <name>A0A1E3PB68_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Antiviral helicase SKI2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_82635</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041860.1">
        <property type="nucleotide sequence ID" value="XM_019185413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB68"/>
    <dbReference type="STRING" id="683960.A0A1E3PB68"/>
    <dbReference type="GeneID" id="30202659"/>
    <dbReference type="OrthoDB" id="64767at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055087">
        <property type="term" value="C:Ski complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070478">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000354">
        <property type="entry name" value="ATP-dependent RNA helicase SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000987">
        <property type="entry name" value="DEAD/DEAH box RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.30:FF:000001">
        <property type="entry name" value="Ski2 ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048392">
        <property type="entry name" value="MTR4-like_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016438">
        <property type="entry name" value="SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012961">
        <property type="entry name" value="Ski2/MTR4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048727">
        <property type="entry name" value="Ski2_beta-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040801">
        <property type="entry name" value="Ski2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE SUPV3L1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08148">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21408">
        <property type="entry name" value="MTR4-like_stalk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21409">
        <property type="entry name" value="Ski2_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17911">
        <property type="entry name" value="Ski2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005198">
        <property type="entry name" value="Antiviral_helicase_SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01142">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="298"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="576"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="495"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="511"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1251" mass="141392" checksum="8EB3BEBD4C28383B" modified="2017-01-18" version="1">MLNESLNTEIPNEPIDPTSKTKWTRPVKSDPDQDLKDRFLNPSNVLPWDLLDLIQDVPEQDPKEFYDALLSVPKTISRSSYRLKRKGIEGKVYNYAEEVNATDLQQSNASTSLSINRKYTSKESAIKGSTSFLPFQPGGIQMSNQLKDNTAMLHRDSDGLFDIAQGLSRGLNLGDSQSVDDLKKLEEVAENEEQPDHDGEIKGIESGDVLESLSSLTTEKSSNNTTVNSKFEKEIDELLPVDINFGRTLVAKEFETERRKDWAHVVDLDHKIENFHELIPNMAREWPFELDTFQQEAVYHLEQGDSVFVAAHTSAGKTVVAEYAIAMANRNMTKAIYTSPIKALSNQKFRDFKQDFPEVDVGLITGDVQINPEANCLIMTTEILRSMLYRGADLIRDVEFVIFDEVHYVNDIDRGVVWEEVIIMLPDHIKFILLSATVPNTFEFANWVGRTKHKDIYVISTPKRPVPLEINIWAKNKLFKVIDADKKFVESSFNQHKEHLEGPKASQNNARGGGAAGRGGRGGARGGTVARGGNRGGRGGNSNGGFGRGGSRGGGNTGNNFKRFQVDRPNKNTWIELVQFLNSNSLLPAVIFVFSKKRCEEYANTLGSVDFCNAKEKSQIHMFIDKAVARLKKEDRELPQILAVREMLSRGIAVHHGGLLPIVKEVIEILFSKTLIKILFATETFAMGLNLPTRTVVFSELRKHDGINFRDLLPGEFTQMSGRAGRRGLDKTGTVIAMAYNDPLSSLKFKDVTLGVPTKLVSQFRLTYNMILNLLRIEALRVEEMIKHSFSENTSQTLLPEHQSKLKELEKKLEEIPVSDEDLDPSSDIQQIHSAIIQYKRVTGQVMAEALTHPLLAKNFNPGRLIVFKDNKGISRLGFIIKQNRVSATPSFSVLTFDMGAAENEEPSGMPFLTQIPGFVQKHFKKIVYNGNIHIREVSVTDIELLCRVSLKARLNEILKSNQEEIKVLTGQLHVLLKFQHRLEEIDWQKFATLKLHELVRERQHVVHKIADITLRTGSSFDEKFKTINQRYEISQQIASLNALISDENLELLPDYEQRLEVLKDLEFVDNNLNVVLKGRVACEINSGWELVITELVLDNFLGDFEPEEIVALLSAFVYEGRVNEEEEAKITPRLEKGKQRIVDIMGKVLDVYTKYQVSLTSEEQEFLERKRFALVNVVYEWARGMTFKEIMELSVEAEGTIVRVITRLDEVCREVKSAALIIGDSALHSKMTHAQEKIKRDIVFCASLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBG0</accession>
    <name>A0A1E3PBG0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">(S)-ureidoglycine aminohydrolase cupin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_76916</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041960.1">
        <property type="nucleotide sequence ID" value="XM_019184467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBG0"/>
    <dbReference type="GeneID" id="30201713"/>
    <dbReference type="OrthoDB" id="4985585at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010424">
        <property type="entry name" value="EutQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36169:SF1">
        <property type="entry name" value="ACETATE KINASE EUTQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36169">
        <property type="entry name" value="ETHANOLAMINE UTILIZATION PROTEIN EUTQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06249">
        <property type="entry name" value="EutQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14036" checksum="7E4C53CE538FF708" modified="2017-01-18" version="1">MPLKHTPATEGFLVKQAPIPSPGNNTFVGDFFTSDAPEGQQMTCGFYQQSVGEPLEYEYEYDEMKVVLAVKADHFYVSDEAGQKIDAKEGDIFYFKKGVTIKFEVVGPEGSYAKNFFTAQRKPDSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBN3</accession>
    <name>A0A1E3PBN3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-isopropylmalate synthase</fullName>
            <ecNumber evidence="6">2.3.3.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_82757</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.13" evidence="6"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042040.1">
        <property type="nucleotide sequence ID" value="XM_019185433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBN3"/>
    <dbReference type="STRING" id="683960.A0A1E3PBN3"/>
    <dbReference type="GeneID" id="30202679"/>
    <dbReference type="OrthoDB" id="418791at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07942">
        <property type="entry name" value="DRE_TIM_LeuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000045">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.270:FF:000002">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00572">
        <property type="entry name" value="LeuA_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005668">
        <property type="entry name" value="IPM_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054692">
        <property type="entry name" value="LeuA-like_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039371">
        <property type="entry name" value="LeuA_N_DRE-TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00970">
        <property type="entry name" value="leuA_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002991">
        <property type="entry name" value="PRK03739.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46911">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46911:SF2">
        <property type="entry name" value="2-ISOPROPYLMALATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22615">
        <property type="entry name" value="IPMS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89000">
        <property type="entry name" value="post-HMGL domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="13">
        <location>
            <begin position="44"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ62833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="596" mass="66162" checksum="EC45CB9446281311" modified="2017-01-18" version="1">MFKNATKTKPYANMLKDPSTKYKPFPQVKLPNRQWPNNTIKKAPRWLTTDLRDGNQSLPDPMSVEQKKEYFHKLIDLGFKEIEVSFPSASQTDFDFTRYAVENAPDDVKIQVLVQSREHLIRRTVESVTGAKNATIHVYLATSDLFRDVVFNMSQQEAVEKAVEATKLVRSLTKDDPNQQATNWSYQFSPECFSDTPSEFAVEICEAVKAAWEPSVENPIIFNLPATVEVATPNIYADQIEYFATHISEREKVCISTHCHNDRGCGVAASEMGVLAGADRVEGCLFGNGERTGNVDLVTVALNLYTQGVSPELDFSDLESVIEIVEKCNKIQVPARAPYGGELVVCAFSGSHQDAIKKGFEVQKKRNAEGDYQWKIPYLPLDPKDIGRDYEAVIRVNSQSGKGGAAWVVLRSLGLDLPRQLQISFSTFVQSEADKVGRELKSEEIITLFKQQYNFNNDGAIKLADYDIVRDPENKDNRFFIGEISVNGEPVSIKGVGNGPISSFVDAMSNFINVRLEVVNYSEHSLGSGSQTQAATYIQLGYMNDENVKTSKWGIGVHEDVSNAAFTSVLSTVNSLVTKGDFELPEIDAQSAPAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBN5</accession>
    <name>A0A1E3PBN5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_88595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041995.1">
        <property type="nucleotide sequence ID" value="XM_019186169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBN5"/>
    <dbReference type="STRING" id="683960.A0A1E3PBN5"/>
    <dbReference type="GeneID" id="30203415"/>
    <dbReference type="OrthoDB" id="4092749at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="684"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="739"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="776"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="810"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="951"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="194"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="518"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="571"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="581"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="631"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="660"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="740"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="778"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="833"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="962"/>
            <end position="990"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1030" mass="112947" checksum="2C022B20D1245859" modified="2017-01-18" version="1">MGAGDNPKRRSLLFPFAKSTPKETKFEVVNRKKNPAANLNRPSAPSPFDSQPSRPPEKRTSSPSPASSASMLASSASRAKSPPASTTSPRSTSPHHIPLDSRAINPFKAHHHTSSSKKDAISQADLPSSNLDVNFNVPPVPDYNSTLHAPPVTIKGRTVSLTKKDAPPRRKPPTLGTVVDPQINNKSVSDLSIQSAGSDSYSYQSSGLDSRLANDQTNARSVEETTLEDDDENVLLPTIQKPEPAAPPSIVVTEELLSAVPKDTEDEELEQVEQKTADLKLSEPLDEEPEVPAKIETQVEELDDIETSSIQDEPELNIKPIAPFSDPSKNTSQVDSFTSASEFMDTEQVEQVPKVEPKNETQYDDDPDSSFEIKPLFFHKKSKSSASEGALRNDASIFTTEGVVTSPKPTHAKQLSLSDDILKDIEDFQSAIPSHVQDETDEVSPVSPLALQKDTRPTIGRTGLYDEISEDEDYSFEQDVPQRQLVVQNPDSEIEDTNESLFQDSSDEGHQPGNYNAFDDKEELKQSESYDYDHVGGMNLGSRGSSVQPEIDYHNNDNNAPKSDHQRIPLTDDETGEEEESSKVYENEFNKFADEAPDTTSSYEGRLAIPRSHSYHNIPSDVASHNEKESYNSNTGSSPLKSTTSEEDGDKLTFERRSVGNASSDQASSYNYYSQAAQTRKFHVVNTGDYSDEDQSSYNPSFDRDHPESLDHDFSVVHQQITNSSDRGRDTDEEDLLSFTAKSPQQTDFNFENKTTPQQMMGNNVSDVDLSQPAKITPVSNLDSPTSITSPVNSYDRREDTVAPVQYVSTYNGSSRRPPPLSDAPKTTRVARSLSTSVPPPVNYNTLQEGIAAAETIHPVETHEPEKSSYVEMLRMSSGTANSDVPASVWGLPIGIADIDHSKLTATASRTAYKRAQRTSKSDLKHGKIKPRLLASEVDDDDSTEMVSGIESQSIDLDKSRTPSIHSPTQSYLNFSPTRTPTNTGVSRSGTLIGDKIGTQLGRSGSVMSTVKPVNNMTLFIANPDIEEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC27</accession>
    <name>A0A1E3PC27_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HCP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_12042</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042173.1">
        <property type="nucleotide sequence ID" value="XM_019180517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC27"/>
    <dbReference type="STRING" id="683960.A0A1E3PC27"/>
    <dbReference type="GeneID" id="30197763"/>
    <dbReference type="OrthoDB" id="272077at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000707">
        <property type="entry name" value="Chitin synthase regulatory factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051726">
        <property type="entry name" value="Chitin_Synth_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430:SF1">
        <property type="entry name" value="CHITIN SYNTHASE REGULATOR SKT5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430">
        <property type="entry name" value="PROTEIN SKT5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="45501" checksum="3235A603A0BA5A99" modified="2017-01-18" version="1" fragment="single">QGHSSTSSKRHNRMSKSVDLSHLYLLDRNESTHFTLTNESLSDVSHNLIKQYLGENSSSSLLPRMKTIEMYRKNVKKSNDPKVLFQYAQYMLQTALTIDSIDNDTNQEEAQLKKQFLKEAVHYLKKLSDKGYTDAQYLLGDAYSSGALGKVENREAFNLFQIAAKHGHVESAYRTSYCYEEGLGVSRDSRRAIEFLKFAASKNHPASMYKLGVYSFYSRMGLPDNVNVKKNGIQWLTRAVTKANELTNGAPYELGKIHENGFLDIVIPDRKYAMELYIQAASLGHVKSAAILGKAYEIGDDIIPQDSDLSIHYYTQAALGGDSESMLSLCAWYLVGNPPNLEKDEREAFQWALRAANSNLPKAQFAMGNFYEKGLGCDKDIEMAKNWYKRAAENNDERAKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC59</accession>
    <name>A0A1E3PC59_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Maintenance of telomere capping protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_77143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042192.1">
        <property type="nucleotide sequence ID" value="XM_019184506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC59"/>
    <dbReference type="STRING" id="683960.A0A1E3PC59"/>
    <dbReference type="GeneID" id="30201752"/>
    <dbReference type="OrthoDB" id="5594977at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018814">
        <property type="entry name" value="DUF5427"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28265">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28265:SF1">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10310">
        <property type="entry name" value="DUF5427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="48792" checksum="D6F1BDB83711AB73" modified="2017-01-18" version="1">MTSTPKKSSEAEDVLEFLASLPESDQSKLKDLKDKKAKNGNADDSDILDFLDELEANDQKKKKTKDIKSEEKKDNNQQEPKQKEEKSEEKPEEETKGQTEVSKDTDNNNDDELPELNDPITSLSNWWSSSGSATVNSFWSNATQQANNFAQKATEEASKFAKDNNLDESFKKLQNLQIDEETRKKILNNNEEINLPDPSRAFGFLTKNLTNVLNSIVQTDEILKIQLIHDLENFNNLDQLLTKNFRKVIKQVQGGIKIELNKNSTLRNHNNRNLNLFNGKLIDGEKLAFANLENSINSFKQEEIEDEVRSSKLFISILPIHISSTSGNKDDIVPIDNQSSGSFSFTIILKDITHDITIITRSQAFPIKWAHWLDGSVNTDDSDQEKNEDENQDEDVDPSEWVKDWIDDGLSLTFGVLAQTYVVKRMGYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC72</accession>
    <name>A0A1E3PC72_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_99298</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042226.1">
        <property type="nucleotide sequence ID" value="XM_019186687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC72"/>
    <dbReference type="STRING" id="683960.A0A1E3PC72"/>
    <dbReference type="GeneID" id="30203933"/>
    <dbReference type="OrthoDB" id="2444812at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="7"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="74"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ63019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="186" mass="20453" checksum="AA35C1D9B2A7E801" modified="2017-01-18" version="1">MSGKGAPKVPDGWKAQFDDKYQTWFYVNLKSGKSQWEAPEGTTFEDDKEKVSPPPYAPGNQTSDPSKQQEPVSQQPSGYQRGPQQQYPQHPQAGYGYPPQQGYYPQQGYPPQQGYPPQGYPQQGYYPQQQQQQQQSTGLLGGMALSSMMQPDIVYAQPPPGDYGGDYGGDYGGDYGGGDDFGGGDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC77</accession>
    <name>A0A1E3PC77_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">Pyruvate kinase</fullName>
            <ecNumber evidence="6 17">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">WICANDRAFT_88059</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the pyruvate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="6 17"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041694.1">
        <property type="nucleotide sequence ID" value="XM_019186136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC77"/>
    <dbReference type="STRING" id="683960.A0A1E3PC77"/>
    <dbReference type="GeneID" id="30203382"/>
    <dbReference type="OrthoDB" id="108365at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00288">
        <property type="entry name" value="Pyruvate_Kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.33.10:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004491">
        <property type="entry name" value="PRK05826.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004978">
        <property type="entry name" value="PRK06354.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14 17" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0630">Potassium</keyword>
    <keyword evidence="15" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="18">
        <location>
            <begin position="25"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="19">
        <location>
            <begin position="386"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="20">
        <location>
            <position position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ62487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="455" mass="50115" checksum="C3E4BFB44922BF7A" modified="2017-01-18" version="1" fragment="single">MAYENSKIGWLASLNVSETPESQLRRTSIIGTIGPKTNTPEILVNLRKAGLNVVRMNFSHGSYEYHQSVIDNARKSEELYPGRPLAIALDTKGPEIRTGTTRGDTDYPIQPNHEMIFTTDEKYLKESDDKIMYIDYKNITKVIEKGRVIYVDDGVLSFEVLEVIDEQTIKVKSVNAGKISSHKGVNLPNTDVDLPALSEKDKADLRFGVKNGVHMVFASFIRTGDDIKVIREVLGEDGKDIKIIAKIENQQGVNNFDDILKETDGVMVARGDLGIEIPAAHVLVVQKQLIAKCNLAGKPVICATQMLESMTYNPRPTRAEVSDVGNAILDGADCVMLSGETAKGNYPIEAVTMMHHTALIAEKAIAYAPLYDELRRLTKRPAGTVETVALAAVNAAAENNAKAIVVLSTSATTARLVSKYRPDLPIIMVTRNPRAARFCHLYRGVYPFVYDKAPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCC2</accession>
    <name>A0A1E3PCC2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_82787</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042081.1">
        <property type="nucleotide sequence ID" value="XM_019185440.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCC2"/>
    <dbReference type="GeneID" id="30202686"/>
    <dbReference type="OrthoDB" id="3981165at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="278" mass="32396" checksum="980AA9AFC050D226" modified="2017-01-18" version="1">MQILENEIPRLSKESTFSDSEVRATTDRIKHFRENFENVQKVEPQTFGNADISLKDLEGFLKDAKKKQISRDEEHFREERAYEIAKLMNNKNARTLDKKNFFTPVDTSLTNKVSSTFRFKPDEKPPASSSKSDELFFPSIRSQKGLTHEFMVLTKSKKIVTEENPLGDDYHPRDLFTIFKTLGNPEKYLKQINKLEKNGGWKLIGSGGDAINKILVFERFIYVKDVLEDLPCHSLQCLCYFMDLVLILNINKKHSIRSMNLEKLSRVPEYLLRFEYAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUB6</accession>
    <name>A0A1E3NUB6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_98048</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035923.1">
        <property type="nucleotide sequence ID" value="XM_019186624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUB6"/>
    <dbReference type="GeneID" id="30203870"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ56716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="152" mass="17072" checksum="8A88CFE3066B3E17" modified="2017-01-18" version="1">MEDKFDTFVETSDNRDDLLIYSMNGVDSNFISDFDPLVVNSQFGILIKNNSSKINHLNVILDSNDQHSDSLILYLLKTFGANVKSINVSIVGENVYKSRLSQILSISVTVQEYDPEKELNGLVLKGLNIGTLADDIEQSKHCDYDLLLLKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUD7</accession>
    <name>A0A1E3NUD7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sorting nexin-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_98094</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Required for retention of late Golgi membrane proteins. Component of the retrieval machinery that functions by direct interaction with the cytosolic tails of certain TGN membrane proteins during the sorting/budding process at the prevacuolar compartment. Binds phosphatidylinositol 3-phosphate (PtdIns(P3)).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Prevacuolar compartment membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036018.1">
        <property type="nucleotide sequence ID" value="XM_019186630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUD7"/>
    <dbReference type="STRING" id="683960.A0A1E3NUD7"/>
    <dbReference type="GeneID" id="30203876"/>
    <dbReference type="OrthoDB" id="5227681at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034499">
        <property type="term" value="P:late endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07295">
        <property type="entry name" value="PX_Grd19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042138">
        <property type="entry name" value="PX_Grd19_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051074">
        <property type="entry name" value="Sorting_Nexin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45963">
        <property type="entry name" value="RE52028P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45963:SF2">
        <property type="entry name" value="RE52028P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="14">
        <location>
            <begin position="78"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ56811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="200" mass="23562" checksum="44351BFE1752C878" modified="2017-01-18" version="1">MSRVFKSFDTEIAENLTPSEPTKQQQQQNQQQQQQQQQQANQNDENTNTKIKNNDQGHQKKSAIKKQTFDELYGEPENFLEIEVRNPRTHGYGRGMFTDYEIICRTNLPAFRLRQSSVRRRYSDFEVFKSLLENENSRVVIPSLPGKIFTNRFNDEIIEQRRIGLEKFLKIVAGHPLLQTGSKILSSFIQDEIWDKGRYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV87</accession>
    <name>A0A1E3NV87_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_70981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036220.1">
        <property type="nucleotide sequence ID" value="XM_019184228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV87"/>
    <dbReference type="GeneID" id="30201474"/>
    <dbReference type="OrthoDB" id="3982491at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="241" mass="28221" checksum="7320AF17AD1C890B" modified="2017-01-18" version="1">MSLLNEKSQLVDENGLDIETLNILRRNSEIEMLNALELKKHELDKYKLKLITKTNQASNQILETRHSMTIENKILNFFIFAFTIFIVLQFSLISIMKFDIDWPSTFQFINFEEFYQISWLCILIIGKMLSYFCLLNFLIRCYIGVKIFKTSVKTNILIKLLNYQFMGFFKSTLITMGIISADIMMKNLLVSMDYVDNHERVNQVLDIVKQITSYGDVVLYSILIVGGIVSVIYRITRMIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVE4</accession>
    <name>A0A1E3NVE4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rad21/Rec8-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_36050</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036376.1">
        <property type="nucleotide sequence ID" value="XM_019182391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVE4"/>
    <dbReference type="STRING" id="683960.A0A1E3NVE4"/>
    <dbReference type="GeneID" id="30199637"/>
    <dbReference type="OrthoDB" id="10071381at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030892">
        <property type="term" value="C:mitotic cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039781">
        <property type="entry name" value="Rad21/Rec8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006910">
        <property type="entry name" value="Rad21_Rec8_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585:SF69">
        <property type="entry name" value="FI11703P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585">
        <property type="entry name" value="SCC1 / RAD21 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04825">
        <property type="entry name" value="Rad21_Rec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="96" mass="10722" checksum="0161F22965B016C7" modified="2017-01-18" version="1" fragment="single">MFYSENLLSKEGPLAQVWLAANLERKLSKNQFLQSNIVQSTKAIANASSQNDESEALALRLSGQLLYGVVRIYSRKAKYLLDDVSDALLKLKSAFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVL2</accession>
    <name>A0A1E3NVL2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hcy-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_35916</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036352.1">
        <property type="nucleotide sequence ID" value="XM_019182372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVL2"/>
    <dbReference type="STRING" id="683960.A0A1E3NVL2"/>
    <dbReference type="GeneID" id="30199618"/>
    <dbReference type="OrthoDB" id="261426at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008898">
        <property type="term" value="F:S-adenosylmethionine-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033528">
        <property type="term" value="P:S-methylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.330">
        <property type="entry name" value="Homocysteine-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017226">
        <property type="entry name" value="BHMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003726">
        <property type="entry name" value="HCY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036589">
        <property type="entry name" value="HCY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051486">
        <property type="entry name" value="Hcy_S-methyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007020">
        <property type="entry name" value="PRK09485.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46015:SF1">
        <property type="entry name" value="HOMOCYSTEINE S-METHYLTRANSFERASE-LIKE ISOFORM 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46015">
        <property type="entry name" value="ZGC:172121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02574">
        <property type="entry name" value="S-methyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037505">
        <property type="entry name" value="Betaine_HMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82282">
        <property type="entry name" value="Homocysteine S-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50970">
        <property type="entry name" value="HCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Hcy-binding" evidence="7">
        <location>
            <begin position="1"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR037505-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="300" mass="33343" checksum="D935D183BC4A4187" modified="2017-01-18" version="1">MSLRERLSKPQPLILDGALGTLLPDISQSHPLWSTHTVLTSPSTIKSIHESYIKNGAQLIQTSTYQTSERGLNDSEFDVSFSDALKRSIDLAYEAKGGRDDVWLVGSVGPYGASLANGAEYTGDYGDISDEGLINFHTERISVLCNDNRVDIIGLETMPNKRELRILLELMDSYDKDYYLSLSVNGEKLADGTTIEELLGLISKGKRLIAVGVNCLSLQSSLEWLKRLKESEKMLIVYPNSGEMYDGINKKWIENPNGSMSWKDYVQKLKAIGNVKIIGGCCRTTPNDIKQISDLVSELW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVM4</accession>
    <name>A0A1E3NVM4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_16339</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035949.1">
        <property type="nucleotide sequence ID" value="XM_019180686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVM4"/>
    <dbReference type="STRING" id="683960.A0A1E3NVM4"/>
    <dbReference type="GeneID" id="30197932"/>
    <dbReference type="OrthoDB" id="4089784at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013240">
        <property type="entry name" value="DNA-dir_RNA_pol1_su_RPA34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053263">
        <property type="entry name" value="Euk_RPA34_RNAP_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28155">
        <property type="entry name" value="ACR243WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28155:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA34.5-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08208">
        <property type="entry name" value="RNA_polI_A34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ56742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="139" mass="15995" checksum="171D76EA8921FBE8" modified="2017-01-18" version="1" fragment="single">SRYQPPKGYHKVKDLKTIPNLDKKEIWLIKTPKGFNFDKLQQLPISSNFKVDSKQFIIQEDLTGINSNSKASNSDKYSLIVPKSNKKAFKINNGLKINKFFNVKESVEIPNIQYGKVVKKRKDVRVIKGLKPQHFATGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVQ0</accession>
    <name>A0A1E3NVQ0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1,3-beta-glucan synthase</fullName>
            <ecNumber evidence="3">2.4.1.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">1,3-beta-D-glucan-UDP glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_36361</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;3)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;3)-beta-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21476"/>
            <dbReference type="Rhea" id="RHEA-COMP:11146"/>
            <dbReference type="Rhea" id="RHEA-COMP:14303"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37671"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.34"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 48 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.34" evidence="3"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036400.1">
        <property type="nucleotide sequence ID" value="XM_019182425.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3NVQ0"/>
    <dbReference type="GeneID" id="30199671"/>
    <dbReference type="OrthoDB" id="1880850at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000148">
        <property type="term" value="C:1,3-beta-D-glucan synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003843">
        <property type="term" value="F:1,3-beta-D-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006075">
        <property type="term" value="P:(1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051278">
        <property type="term" value="P:fungal-type cell wall polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026899">
        <property type="entry name" value="FKS1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056261">
        <property type="entry name" value="FKS1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003440">
        <property type="entry name" value="Glyco_trans_48_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741:SF48">
        <property type="entry name" value="1,3-BETA-GLUCAN SYNTHASE COMPONENT FKS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741">
        <property type="entry name" value="LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14288">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23605">
        <property type="entry name" value="FKS1_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02364">
        <property type="entry name" value="Glucan_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01205">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="471"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="516"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="558"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="587"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="648"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="706"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1384"/>
            <end position="1405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1471"/>
            <end position="1491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1497"/>
            <end position="1515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1587"/>
            <end position="1610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1622"/>
            <end position="1644"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1665"/>
            <end position="1687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1825"/>
            <end position="1845"/>
        </location>
    </feature>
    <feature type="domain" description="1,3-beta-glucan synthase component FKS1-like" evidence="13">
        <location>
            <begin position="325"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ57193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1900" mass="217764" checksum="633F25BD30D75B77" modified="2017-01-18" version="1">MSYNNDQNQHYYDQQGQYNPQQDAQYDQYADQYNQQYEDPNGQQPYYNDGYHASQEGLNNGEYYDPQNDASYYSQGYDPATGAAYDQGYNQEAYYDQPRGGANDHEAFSDFSYGAPNAPGTPGYDSYGTQYTPSQMSYGGPRSSGASTPIYGSNGAFDPSSIAVALPNEPYPAWTADQQAPIAIEQIEDVFIDLTNRFGFQRDSMRNMFDHFMTLLDSRSSRMSPAQALLSLHADYVGGDTANYKKWYFAAQLDLDDEVGFRNMNLGKLSRKARKAKKKSKKARKAVEEHGPENTEETLNALEGDNSLEAADYRWKAKMNNLTPIERVRHVALYLLCWGEANQVRFTSECLCFIYKCALDYLDSPACQQRVEPVPEGDYLNRVITPIYRFLRSQVYEVVDGRYVKRERDHNKVIGYDDVNQLFWYPEGIAKIVFEDGSRLVDLAPEERYVRLGEVSWDMVFFKTYKEIRTWFHLVTNFNRIWIIHGSVFWMYMAYNSPTLYTKNYQQLVNNQPVAAYRWASAALAGTLASFIQIVATICEWFFVPRNWAGAQHLSRRFWFLILIFAVNLGPIVFVFAYDPLTVYSKAALTVSIIMFFVAIATIVFFSVMPLGGLFTSYMKGSTRKYVASQTFTASFAKLHGLDMWMSYLLWTIIFFAKYIESYWFLILSLRDPIRILSTMEMTRCVGEYWWGSVLCKQQARITLGLMYMTDLILFFLDTYMWYIVCNCVFSVGRSFYLGISILTPWRNIFTRLPKRIYSKILATTDMEIKYKPKVLISQVWNAIVISMYREHLLAIDHVQKLLYHQVPSEVEGKRTLRAPTFFVSQDDNNFDTEFFPRNSEAERRISFFAQSLATPIPEPLPVDNMPTFTVMTPHYSEKILLSLREIIREDDQFSRVTLLEYLKQLHPVEWDCFVKDTKILAEETAAYENGDEEKDENGLKSKIDDLPFYCIGFKSAAPEYTLRTRIWASLRSQTLYRTVSGFMNYARAIKLLYRVENPEIVQMFGGNAEGLERELEKMARRKFKFIVSMQRLTKFKPEELENAEFLLRAYPDLQIAYLDEEPPLNEGEEPRIYSALIDGHCEILENGRRRPKFRVQLSGNPILGDGKSDNQNHALIFYRGEYIQLIDANQDNYLEECLKIRSVLAEFEEMNVEQTNPYAPGLKFEEQNKNHPVAIVGAREYIFSENSGVLGDVAAGKEQTFGTLFARTLAQIGGKLHYGHPDFINATFMTTRGGVSKAQKGLHLNEDIYAGMTALCRGGRIKHSEYFQCGKGRDLGFGSILNFTTKIGAGMGEQMLSREYYYLGTQLPLDRFLSFYYAHAGFHINNLFIQLSLQMFILTLVNLNSLAHESIMCSYDRNKPVTDILYPLGCYNLAPAIDWVRRYTLSIFIVFFIAFIPIVVQELIERGVWKATQRFFRHLLSLSPMFEVFVGQIYSSSLLNDLSVGGARYISTGRGFATARIPFSVLYSRFAGSAIYMGTRCMIMILFGTVSHWQPALLWFWASLSSLMFSPFIFNPHQFAWEDFFIDYRDFIRWLSRGNNKWHRNSWIGYVRMSRSRITGFKRKVLGLESEKAAGDASRAHRTNIFFAEIFPTVIYAAGCFVAFTFINAQNGVKHVDPVNSVLRVIICTLAPIVIDLGVLFLCMGLSCCSGPLFGMCCKKTGSVMAGFAHGVAVIVHIIFFIVMWVLEGFNFTRMLMGVITCIYCQRLIFKLMTALMLTRELKSDNANTAFWTGKWYGKGMGWAALSQPARELCAKVIELSEFAADFVLGHIILFVQFPLVLVPLMDRWHSVMLFWLKPSRQIRPPIYSLKQSRLRKRMVRKYLALYLLVLIIFAACIVGPAVAASHVPSNIGSSLTGVFANLVQPRHQWNNDTGPNATSLTGYYFTSTPSLSTWSTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVW5</accession>
    <name>A0A1E3NVW5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetylglucosaminylphosphatidylinositol deacetylase</fullName>
            <ecNumber evidence="2">3.5.1.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_65714</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIGL family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.89" evidence="2"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036039.1">
        <property type="nucleotide sequence ID" value="XM_019183972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVW5"/>
    <dbReference type="STRING" id="683960.A0A1E3NVW5"/>
    <dbReference type="GeneID" id="30201218"/>
    <dbReference type="OrthoDB" id="440160at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000225">
        <property type="term" value="F:N-acetylglucosaminylphosphatidylinositol deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993:SF11">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ56832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="293" mass="33549" checksum="C04E4FDAA77CE6B6" modified="2017-01-18" version="1">MVFKYLIRKTRRIVLLLFTSFLVWGLLATFFQQFPSSADSIATSSTTFTLNKIPKSLQSRTIQVLIAHPDDEVMFFAPSIVELSKPKYKNNITLTCLSVGNDQGLGETRYNELVRSLQILGIEDFEIVNDETKFKDSMELEWDADKIAEYISKDTDVILTFDKFGISNHPNHKSLYHGAIASKKPVYALKSWNMAEKYSSTLCTNAEIMLKLFDHSILYLVDAFHIESYLPVNLWAILHEVLYKNVHIYADLPSTILGIAAMSNAHQSQMVWFRWGWLSVSKYGNSNELIKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW74</accession>
    <name>A0A1E3NW74_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_64740</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPL family. K-HECT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036593.1">
        <property type="nucleotide sequence ID" value="XM_019183795.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3NW74"/>
    <dbReference type="GeneID" id="30201041"/>
    <dbReference type="OrthoDB" id="423283at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904855">
        <property type="term" value="F:proteasome regulatory particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010994">
        <property type="term" value="P:free ubiquitin chain polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035519">
        <property type="term" value="P:protein K29-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045322">
        <property type="entry name" value="HECTD1/TRIP12-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="8">
        <location>
            <begin position="1184"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="681"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1144"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="76"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="131"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="684"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="708"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1144"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="1503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1536" mass="174702" checksum="8EC2CBF47F336E3B" modified="2017-01-18" version="1">MARRKETGSPVDKRKTRSQGPAAAESCDLDNDMVMADGNEDNAENHQSDQDIEHHQVEEDEDEEEDEDASLVRLSEQYRRFSESQRELEQLENNTHENNDNDHHIEDEDDDDDHNDTDDNDEDEDDSQSEDMMRSIHRANEDHEDDYDHSDEDEEEGDLENELLQGAFRSFANRLQRSNESNDNPRGEARSNAANSLAENMSRMFPEAMGLFGGFGAGGAGGSGLRISGLIDGLKRRDDPYLVLETLNELSERLLMMNGLVAERGLPAHDLAHGLVDVLTDPFFMDQLELQLVACRCLYNFLEINNDYVHSAVHAGAIEALQSKLLEISYIDLAEQSLQTLEIISKISGTQIFRRNCLLSCLQYLDFFTIHAQRKAINITANSVKYIKESEFEIVEEIFPILQRIVVEFSDSQALNNSWLAISRIIKNFRSSPNLLTKLINTDLLNKMISVISNPDTNITTNLRLINTLSICARNDELSILILKGGKIGDCFKSSLSKYKKSNNQDDSVSIEALMAAPKDLIISILDLIINLLPSEKNSILTIQFVEKNYSSIQSFYEIFINEIFQLLINIYSSTVIFDIRRRVMISLIRIVSSLDDLTKLGNHSQVVNLLASIVIQNKSIIRQEKKDQKPYMVLLGSLILTQLLAEKDPEKYLLEFEREGLIADTSSLLEILRVDEKTVKKDDDDETMEEDTQQQDEEQRNSYPSSEVDEGVYDEEEDEEDEDEEFNNRQLFNVSDQAIFKNLTTPQIIKHLTKYASDIEQEYLSKKASGSSKLEHLRLLDEISAVLKDTEQIDSYNYNDWVQIWNNFKKALGDEKTDSTISSFELISSGFIEILSSLFSNDVTGDQKSICRNSFLDVFNHEAFELLIKKLQEALTRSESFEILSCGLKTGENRAASLGKQIKVKLIAEDPNDEQFEGMSAFIILVHAIASFNNINSFLKRRVETPFFFRQRNSNNRDENRRRSDWHIEFLIDDEVIPYDSTIYGALFKNSQAKNPNFDSSRFWFETHEIKFRKVEGVAPVADFDDRYPGLNAGEDEDEDDEDVENLKHPVTLSILSLLKILHDFDDSTSKSFLNFKLTAKLNRQLEEPLIVASGILPSWAINITRKFPFLFPLDTRIFFLQSTSFGYSRLIQLWTARANQDQNNAENDNNGNNNNGNGSQPPLGRPTRQKVRVPRNQLLQSSFKVLDKLGSHPSILEVEFTNEVGTGLGPTLEFYALVSKLYCQKSLGIWRQHDEIDGDDYVNNKNGLFPSPIVSDGKERLSSFKYLGKFVARALLDNRIVDFYFNKVFFQIAQSIINKEPIIKDLEVLKLVDEGLYNSLIYLQKNKDLVEGLEINFTLPGFPSIELIENGSDINVDSNNVDDYISKVVDFTIGLGVEKQVESFIKGFSEVFPYSSMLIFSPNELVELLGNANEDWSYETLISAIHADHGYTVDSPSVQNLITLMTKFSKQERRLFLQFLTGSPRLPIGGFKNLKPVFTVVLKHSEGDLKPDNYLPSVMTCANYLKLPNYSSSDILEERLMKAVNEGAGAFLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWJ1</accession>
    <name>A0A1E3NWJ1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit</fullName>
            <ecNumber evidence="4">2.4.1.198</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">GlcNAc-PI synthesis protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_85657</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalytic subunit in the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.4.1.198" evidence="4"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036713.1">
        <property type="nucleotide sequence ID" value="XM_019185922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWJ1"/>
    <dbReference type="STRING" id="683960.A0A1E3NWJ1"/>
    <dbReference type="GeneID" id="30203168"/>
    <dbReference type="OrthoDB" id="734129at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000506">
        <property type="term" value="C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017176">
        <property type="term" value="F:phosphatidylinositol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03796">
        <property type="entry name" value="GT4_PIG-A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000053">
        <property type="entry name" value="Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000026">
        <property type="entry name" value="Phosphatidylinositol N-acetylglucosaminyltransferase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039507">
        <property type="entry name" value="PIG-A/GPI3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013234">
        <property type="entry name" value="PIGA_GPI_anchor_biosynthesis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45871">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45871:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08288">
        <property type="entry name" value="PIGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="392"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="PIGA GPI anchor biosynthesis" evidence="13">
        <location>
            <begin position="42"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="12">
        <location>
            <begin position="187"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ57506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="439" mass="49477" checksum="3706BB05742530F6" modified="2017-01-18" version="1">MPYNIAMVCDFFYPQPGGVEFHMYHLSQKLIARGHSVVIITHSYNSRRGVRTLTNGLKVYYIPFLVFYRQTTFPTVFSAFPILRNIFIRESIDIVHGHASMSSLAHEAVFHGSIMGLKTVLTDHSLYGFADTGSILGNKALKFTSCCTDRVICVSNTCKENIVLRGAIDPLLVSVIPNAVISSDFLPDPSAPKKNRITIVVISRLFPNKGADLLAGMIPKICQADPNVNFLVAGDGPKFIDIQQTIEKNRLQDRVELIGGVKHEKVRDVMVQGHIYLHPSLTEAFGTVLVEAASCGLLVVTTRVGGIPEVLPSDMTIFANPAVDSLVESTLRAIKLIEERRVNTSKFHDEVKTMYGWEDVAMRTENVYDSIDEDYKLTTLEQFWKYYKCGAWAGKLFVLCFIVDIFLYALLEWWYPRENIDIAKKWPRKNKVQEKKSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWN0</accession>
    <name>A0A1E3NWN0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Short-chain dehydrogenase/reductase 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Retinal short-chain dehydrogenase/reductase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_35648</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036755.1">
        <property type="nucleotide sequence ID" value="XM_019182337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWN0"/>
    <dbReference type="STRING" id="683960.A0A1E3NWN0"/>
    <dbReference type="GeneID" id="30199583"/>
    <dbReference type="OrthoDB" id="10253736at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052650">
        <property type="term" value="F:all-trans-retinol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05339">
        <property type="entry name" value="17beta-HSDXI-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000131">
        <property type="entry name" value="Short-chain dehydrogenase/reductase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322">
        <property type="entry name" value="PKSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322:SF736">
        <property type="entry name" value="RETINOL DEHYDROGENASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ57548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="322" mass="36067" checksum="57B754A3E0C20B36" modified="2017-01-18" version="1">MHFALVIKAQLYRQIVHANWLISYLSDSLIGVVLERHRDVVLVTGGGSGLGKEIVKEFQKRRIQRLVVFDINIPNDDQKLEDVYYYKCDVSDKKQVMELSERVKNEVGIVTILVNNAGITSGKSLIDLSFEEIEKIIQVNLMSSFYLDKAFLPDMLMLKRGYIITVASVLGYMSPANLTAYGASKSALIALHESLTYELGPPLFSSTGIKTLLVCPGQLKTQMFKGVNTPSSILAPELDPQYVAQKVVRAVDYGRRGELKFPIYGNFLALFRSSPWPVTQFVRLISGMDESMKTYEQDKQDRINQKKANQAAILGEIISSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWR7</accession>
    <name>A0A1E3NWR7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Histone-binding protein RBBP4-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_34831</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036795.1">
        <property type="nucleotide sequence ID" value="XM_019182241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWR7"/>
    <dbReference type="STRING" id="683960.A0A1E3NWR7"/>
    <dbReference type="GeneID" id="30199487"/>
    <dbReference type="OrthoDB" id="427795at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033186">
        <property type="term" value="C:CAF-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990426">
        <property type="term" value="P:mitotic recombination-dependent replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="152"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="248"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="293"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="340"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="380" mass="43503" checksum="D0219F1CA1B7070B" modified="2017-01-18" version="1" fragment="single">YRRWKTNAVQLYDYLNTVNSIWPSLTVQFFPDVETVEENKKYKYRLLSGTFTSGQREDYLQLGSFTVKSEIEEVDISKYDQDLEEFISSNPISGKFNYDQKILHQGDINKARYMPQKPDLISTINNNGEIFIFDKTKHSSQPSDEFKFEIKLNSHKKEGFGLSWNNFKEGLLLSSSIDGVTKLWDITKYSKNCKIMDTPTNNFTTDSKGTNDVEWIPTHNSIFGTVGEDNIVKIFDTRTNSILKQNEPKHHTGGINALSFNHENQFCLATADSNGILNIWDIRDFSKPIFQTSKAHNGSISTISFNENKPNILATAGADDNLVKLWDFSKEEDNQLIFTHGGHMLGINDIAWNPHDDWMISSVSNDNTLQVWKPSKQLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWT7</accession>
    <name>A0A1E3NWT7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RP21</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">S13</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">YP28</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">YS11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_80925</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. uS4 is involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). Interacts with snoRNA U3. uS11 interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036801.1">
        <property type="nucleotide sequence ID" value="XM_019185144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWT7"/>
    <dbReference type="STRING" id="683960.A0A1E3NWT7"/>
    <dbReference type="GeneID" id="30202390"/>
    <dbReference type="OrthoDB" id="1697570at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000021">
        <property type="entry name" value="40S ribosomal protein S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_uS4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_uS4_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003139">
        <property type="entry name" value="PRK04051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF5">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 13" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 12" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="16">
        <location>
            <begin position="6"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="RNA-binding S4" evidence="15">
        <location>
            <begin position="107"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="162"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00075843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00077547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00080764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00081686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ57594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="192" mass="22122" checksum="22B0C6FFA8EB54EB" modified="2017-01-18" version="1">MPRAPRTYSKTYTVPKRPYESARLDAELKLAGEFGLKNKREIYRINFQLSKIRRAARDLLTRDEKDPKRLFEGNALIRRLVRVGVLSEDKKKLDYVLALRPEDFLERRLQTQVYKLGLARSIHHARVLISQRHIAVGKQIVNIPSFLVRLDSQKHIDFAKTSPYGGGRAGRVARRNKARKSEGGDEAAEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWV7</accession>
    <name>A0A1E3NWV7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Methionine aminopeptidase 2</fullName>
            <shortName evidence="9">MAP 2</shortName>
            <shortName evidence="9">MetAP 2</shortName>
            <ecNumber evidence="9">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MAP2</name>
        <name evidence="13" type="ORF">WICANDRAFT_64974</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9 10">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="9">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="9"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036835.1">
        <property type="nucleotide sequence ID" value="XM_019183842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWV7"/>
    <dbReference type="STRING" id="683960.A0A1E3NWV7"/>
    <dbReference type="GeneID" id="30201088"/>
    <dbReference type="OrthoDB" id="7848262at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01088">
        <property type="entry name" value="MetAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03175">
        <property type="entry name" value="MetAP_2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050247">
        <property type="entry name" value="Met_Aminopeptidase_Type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018349">
        <property type="entry name" value="Pept_M24A_MAP2_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777:SF2">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01202">
        <property type="entry name" value="MAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9 10" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="12">
        <location>
            <begin position="116"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="53"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ57628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="426" mass="48083" checksum="4511A5035A1AB92B" modified="2017-01-18" version="1">MSKVEAEGTNGDKPAESTEKIAENIEKLNVENEEDDGEEEEVNGEGAAPSGEKKKKKKKKNNKKKKKTIVPITQIYPDEKYPEGEWQEYKDTNNFRTTNEEKRYLDRQDDSRWNDFRKGAEIHRRVRKHLNNVLQPGLTTTEIAETIENSVRTFMNEEDTKKAGIGFPTGVSLNHCAAHYTPNAGDKTVLKYEDVMKVDYGIHVNGYIVDCAFTKTFDPKYDTLLKAVKAATNTGIKEAGIDVRLTDIGEAIQETMESYEVELDGETYPVKCIRNLNGHNIAPYHIHGGKSVPIVKNGDETKMEEGEVFAIETFGSTGRGYVIPEGECSHYAKNVDAPHIPLRLNKAKTLLANIDKHFGTLPFCRRYLDRIGEDKYLFALNSLVKAGIVQDYPPLNDSRGSYTAQYEHTILLHPTQKEVVSRGEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWW5</accession>
    <name>A0A1E3NWW5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">Serine/threonine-protein kinase Tel1</fullName>
            <ecNumber evidence="4 16">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">WICANDRAFT_97016</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates histone H2A to form H2AS128ph (gamma-H2A) at sites of DNA damage, involved in the regulation of DNA damage response mechanism. Required for the control of telomere length and genome stability.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Chromosome</location>
            <location evidence="2 16">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the PI3/PI4-kinase family. ATM subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4 16"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036830.1">
        <property type="nucleotide sequence ID" value="XM_019186550.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3NWW5"/>
    <dbReference type="GeneID" id="30203796"/>
    <dbReference type="OrthoDB" id="381190at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05171">
        <property type="entry name" value="PIKKc_ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038980">
        <property type="entry name" value="ATM_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044107">
        <property type="entry name" value="PIKKc_ATM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021668">
        <property type="entry name" value="TAN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11640">
        <property type="entry name" value="TAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="16" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 16" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="12 16" id="KW-0779">Telomere</keyword>
    <keyword evidence="7 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="18">
        <location>
            <begin position="1660"/>
            <end position="2254"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="17">
        <location>
            <begin position="2364"/>
            <end position="2671"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="19">
        <location>
            <begin position="2677"/>
            <end position="2706"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="20">
        <location>
            <position position="2706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU365027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ57623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="2706" mass="308783" checksum="94ABA4E952D9D3FB" modified="2017-01-18" version="1" fragment="single">MLRRLVVQHAKQMKFKLVKQLLNFCKEIIFGYENEIVKPLALDISKILNILVSIDQHRDHISSEDWVRQMDAILKVLNEQIKHNMTEKFVSELLGALKVLVVNPLIAIEDLGFDLSDFLVKYFKTMKKESSLTSVVLDITNNLIITLSTSKVRYSIKLVKSVLKIAGSLVNTNYEALREQILIFGLLSSDFICENLPKLIEVESSQGPSFDDDDGLRDDLIFWIDQWIQHVLRTPTDILSLQHIEFLNSKTENSWFNFGPIRLENSSPPKPWLSTLALTKLVNSLYYLKRPLVKNPEGPAKRQKLGSESYVTTIDTSESPFQLFHRFIQSTDVSMQKLGLQLLTFYVYNYKPVLTAVVFDEIKSYHDKPELAGFTSLLTKALIEAGWELDNTELSQLLRINLQLLKDRNLVNVASQTIIQVLNYPKYTVGENSLYLQMQSVFEISEINGPASVTNDSLRFWVAFSQHGKGFKSANKKVGDRIVDWLLTKWDRESVVEVSSFPYFISWLAGTDFTAEDDEVIYENIFNTFCAKWGQKSSLAKFILRSDSPVPINVSSQKLQKPKIMIKVDSLTPVIDQLLLDMESFDAKSKGYELLRLVRYGFLIYQNIQTLSRLESTVERLKYDIRRFLENLEIDKFDDYNDAVFQISEFKVVNGSGAFIFESLDAQEIAFNALSNNEKLHESMITGGNGPDSSIIMSDFGDFGQVRKETKDSYFQASTTYLDLELYSTFEQKVFKLLILLNSQFSSSKNLNTTLDQALRVLEKLDHNAFSSGLFTLLEYLEKVDVMKIQTVSISKLLRLLGHKLLSSYQTERSPTTIILASRLFTVFTKAWLLKITEKWAVDYSDMFKWIVSLAEKGLIAEEVSLYYFIESIFAKLGFEMDSASLDVAISLFHKCSNLNKVKLCEGIKKFIVILPPSEQTKCLEAIYSGFINPQVLDDSAATYTYCIGQLITVSYPLFIGGISQLLLNYGYLHFAPYIESLFLEIDLDILQQKFILLELLKFWYESRSDLTTFPAQLFGLKNQLEFYDTYKGLIYPILISHKGTPSQLILNNGIMGMSIDESDLLLETLSLSIPLSYTKNGRRSELLKIINETYDYSLIEHQTLLIVEKFMECCDFSKESDFEKYIGSIEKDQTLSLLFNHSSSVDNENSSYSRVNISVPFSVVIHQITQITASSADFWTPAAVLYLSRNVIKSLSSAISEGGRFVLLRRLKLIVTLGFQGFQNQDVAFAVIEFLYPYISDSSIHIDVCSILVVLLKLSKKAVTNTEHYFAVLLMVLHESVRYKIKGSSLFHLLESYLIEENAGIDFKGIWKMLFLPLTHILTQDSSSLEGSLIMDYDSQANLEYPRSEYIIIHDILKYGAIEKSQKMKKLIFNVLSDIFNEVPFLSEIDWRIKTARDVAILLLDFKDGESKEFNLFKARYLGRHYLLTGETLSDLEREIPVTIEYDLDLKTETLFGIFDELLKIRKASEDLKEIQLIDSVFGVAFFEHNRGALKDSPLVSFDQHLKDQVKVLPALDLFTFKLISGNHPAFYSWAQLAKDSEVDVKPTGIWCRDVTLGLLGTFVDHSPIIPALMTYVYRFPEISKSLIFEVLLFYIRCGRSAAEKLALNFINGIIEREDVYQLSNEKIEIIVKICYFLRINRDDIKPFNNVFRNLNLERLFKLACHIGLSKVALLFFELHYTDLSSNKAVKWTDDTKTLRKIYNNFDDADLLHGLPVDPTLGYAMDSFSRGNDSDWKSVIFNSANLDSSIALKEYSNFDVSSSRNMMLRSLVGNSLHGVSELLNDTMDPQKRLRNSYEWCWKLNQWDLPVPESPSTENEAIYKALKTIYDNNKKGQAICNSLLVDSLEVNGKVKSLPLFKTLSSLYSIEIVQSLNSFTIFEEAGNFTDCTEAWFPLVSFTEIENIMLSRRSSYGICGLSGKIGVSDGLVAEILEMKRYGYYARLHGEVQRATNASMWIDKRIQVVSEEDLKRYLTKISNFESACTLWNQGEAAISIAMLKDNLSKQYPTPPEITLASLEIHDSVLNAHLVKWTSESRQEKFETIMQTYVKASLKDIEKVSSAREKAGVYHILGYFCYKQTKLPGAGEEIERQEMRLKSKQAELKDLKVIIQNKSTPADEKKNAKRYFQRVQLQYESDKEAYNILCGNKSTCIEKALEFFMKAASIDDFYDDEDIDKICALWLEYSDDDSINKITRKEISSISSHKFIPWINQLMSRLSDDSTPFQHILLNILVAICLRHPFHSLYFVMNLRVHKLYQFARTDATINSRSAAAEKLWLSLTSQGENFARTVMKPIDTLSRNALSLATEKVPNNNVRKMNLENLKIGDFWINQVGDLRIPLPTINQLKISLDGNYSSVPRIISVDPVIQISSSGISLPKIMKVFLTDGSRHKMLLKGSSDDLRQDAIMEQVFEKVNTILRNDKETRKRNLKIRTYKVVPLGPQAGMIEFVANSIALSDILRPLHSEDSISFSEAREMMRGAQSLNNKERLNVYLKIAKATPPVFRVFFYDTFPNIDEWYRSRQVYTRGIATNSIVGFILGLGDRHLNNVLIDKRTGEPIHIDLGVAFDQGKLLPVPELVPFRLTRDIVDGLGITGVEGSFKKGSEHVYRVLRLNVEKILGILNVLKYDPLYSWAISPVRKKRLQEIENDNVSSKSNVEDDGSDAVRAVQGVEAKLNANGLSDEASVQELIQEATDYKNLGVIYLGWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX26</accession>
    <name>A0A1E3NX26_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_35373</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3NX26"/>
    <dbReference type="GeneID" id="30199542"/>
    <dbReference type="OrthoDB" id="3981051at2759"/>
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    </dbReference>
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        <property type="entry name" value="C630.06c-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09428">
        <property type="entry name" value="DUF2011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="236" mass="27085" checksum="8BA27ED408B532AB" modified="2017-01-18" version="1" fragment="single">RVSRHDLYNSESDIEPSDDGQTPILPNLEFEIIDKTQDDDQVMGDAQEEEEDFDFPLFSMGTTTTTAAKPEEQKDQEEESLRGRTQEKQNTMRITLRSPSPEVIIQERPRSYYFAENDEEVKAKFAQAAITGDDVIRQSLIPYGPIGKVINLNEHNAKIEKELEKINKKARPGKKKRLASIKATEHNKERLKVQKQNEQKAKAKLMKKIHHKRGGKKHKKKEGASDAQGKTKYRTE</sequence>
</entry>
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    <name>A0A1E3NX37_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">WICANDRAFT_97646</name>
    </gene>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ57147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX37"/>
    <dbReference type="GeneID" id="30203849"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="57" mass="6614" checksum="2440DD0C97B88C62" modified="2017-01-18" version="1" fragment="single">ELQLAWISKSILYLYVLCSDISDRIGKDLRQIIISTSLTPDFGLYVVVFKFTELCER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXF7</accession>
    <name>A0A1E3NXF7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase family 62 protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">WICANDRAFT_81446</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ANP1/MMN9/VAN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036434.1">
        <property type="nucleotide sequence ID" value="XM_019185235.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXF7"/>
    <dbReference type="STRING" id="683960.A0A1E3NXF7"/>
    <dbReference type="GeneID" id="30202481"/>
    <dbReference type="OrthoDB" id="204164at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mannan_Polymerase_Subunit"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083:SF6">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083">
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    </dbReference>
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    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
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        <location>
            <begin position="70"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037964"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ57227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="480" mass="55159" checksum="51E61AE15909D999" modified="2017-01-18" version="1">MARFNRPAILVLVLTLLAFTTYYVFEQQKYSLSDLSSFLETMDIGSVGGQGAPATQPKGKITNNNDPDILQKHKIEIQVNELLQKKLENELKYERESMDKKNKQLQEQNEALRKEKTKFEQENKSLSLKVQEAEKGKPVTVYKRERYSEEPSFIRKTFTFKTNPEFSPDVVIDGDKNEVVILASIIEKFDQQGQPFQRFLKLLQSIEYPHYKTSISLLISDNDEFKKFDEFVTDIFNKIEEVKDTSLLKDSFSKITLINADFIESQFKVDKGLRHDPRVQKKRRKLLAQIRNFLVFNGIGSEIYSLALDSDVIELPKNMLETFIESKKDIATIRIDIKNHEGKVVHQDYDLNSWAGDRRVPNAEQDAKLDTDPDFFYEPGPGPNNIQFSHIDKNLEKFGINKDDPKAAIELNSVGGAVLFVKTEVFKQGIMFPPFYLIGTKWNRNDGFDGIETEGLCYQAKTIGYKCWGFPNLVGFHAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXL2</accession>
    <name>A0A1E3NXL2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_80050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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            <taxon>Wickerhamomyces</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037090.1">
        <property type="nucleotide sequence ID" value="XM_019184993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXL2"/>
    <dbReference type="GeneID" id="30202239"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="574" mass="65929" checksum="64FE6A44FC47F98D" modified="2017-01-18" version="1">MLQLLNLPKDVWDLIIFEHGVSFDDLLNLLHTSQSFRPLIERYISVISNDISHPVNGISAKLNGLNANPPSLFTFDSENFDFIISSSVLIIFNIFLEQPNVQLDSFLKMIGQKCTQFRKFFKINLFLSEAYIVEECEFKYVVGDLQTLIHNKEFFNFFQPDSFLEYSVCELDPFDSCAQNRNNNGQIPWVESSYVDKAGWSLLKKVFIELHSNLDMELFKGGKKHPPFPFVLENAKFENLTCLWFSSTKLLFDSSSECYQSNFGFYSKIPACFKNCDFPELTVFWMNRTPTLSFKYACSIKIQSLINCHFDKLTLLKVNPDDFFIVRNVEMNNLQVINLGDSTTASSLFPYMTELTKKYSIDSNGCHNQSDPFYSNVATPKFSYFEVTNLDFGSMKNVVSLSLPSLGLFHDICRTISSLKLEDFKEIKISCGKNNLGTVILLKELNDANIKKVRFKSTTSVFNDSTTHEGREYDIVGLPRVGSIHPLGVRHTLPFRNISTALDCYYSAPGWTSRFSFVPYYSDLEIEVQWSAKRARYIKPPIVQVSPANANDQRIERGAGRVFHYRLEDSIYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY58</accession>
    <name>A0A1E3NY58_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_64081</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037137.1">
        <property type="nucleotide sequence ID" value="XM_019183669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY58"/>
    <dbReference type="STRING" id="683960.A0A1E3NY58"/>
    <dbReference type="GeneID" id="30200915"/>
    <dbReference type="OrthoDB" id="1749473at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050825">
        <property type="entry name" value="RBM42_RBP45_47-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640:SF11">
        <property type="entry name" value="RNA-BINDING PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="82"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="164" mass="18476" checksum="79EC8F5C7712670E" modified="2017-01-18" version="1">MSTQNNRVKKPRVTNFKSSDRRLAAGIRPESFLLSQQHQNVNISPAIQKHQPIIKGTNTKNNKNKKNHNTDPTLEEWDPAHFRLFVGNLGSDANDTILLKSFGIYPSVTKVKVPMDKNGANKGYGFVAFSDPNDYLKAFKELNGKYIGHNPCVLKKANDLKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY84</accession>
    <name>A0A1E3NY84_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Polyadenylate-binding protein</fullName>
            <shortName evidence="13">PABP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_97050</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Binds the poly(A) tail of mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036869.1">
        <property type="nucleotide sequence ID" value="XM_019186557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY84"/>
    <dbReference type="STRING" id="683960.A0A1E3NY84"/>
    <dbReference type="GeneID" id="30203803"/>
    <dbReference type="OrthoDB" id="19742at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140693">
        <property type="term" value="F:molecular condensate scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008428">
        <property type="term" value="F:ribonuclease inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060211">
        <property type="term" value="P:regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12378">
        <property type="entry name" value="RRM1_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12380">
        <property type="entry name" value="RRM3_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12381">
        <property type="entry name" value="RRM4_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000385">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000441">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000520">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006515">
        <property type="entry name" value="PABP_1234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034364">
        <property type="entry name" value="PABP_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01628">
        <property type="entry name" value="PABP-1234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 12 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="58"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="146"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="239"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="342"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="17">
        <location>
            <begin position="549"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="16"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="501"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="630"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="307"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="16"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="645"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ57662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="655" mass="71977" checksum="E256B4A6D3DD437D" modified="2017-01-18" version="1">MSTTVDAVDKTVNELENLNINKEESPVAAESSATPTSAEESTEDPSSGSSQTVAETTASLYVGELEPSINEALLFEVFSPIGQVSSIRVCRDAVTKRSLGYAYVNFHNVKDGEKAIDQLNYTPVKGQPIRIMWSQRDPSKRKNGEGNIFIKNLHPAIDNKALHDTFSAFGRILSCKVATDNFGNSKGFGFVHFESQESANEAIESVNGMLLNNNEVFVGPHVPRRDRQSKYEEAIKSFTNVYVKNIDLEATDDEIKDLFTPFGAITSISVEKDQEGKSRGFGFVNFEEHEAAVKAIEALNDQDFKSKKLYVGRAQKKSERLEELKKQYEAARIEKLSKSQGVNLFVKNLDDSIDDEKLREEFQSFGTIASVKVMTDDTGKSKGFGFVSFSSPEEASRAISEMNQHMLAGKPLYVALAQRKDVRRSQLEQQIQARNQLRMQQAAAAGGLPGQFIPTPFMYGQQPGFLPPGARGPIGGPTPQFMMQPRPGQGIPPQQAGQWAGARQLPNGQPMYGVPQNFEQFPQGRGPNRGGFRGNQRPRNQGPEGQGSNVGQLAQILPTLPEPQQKRVLGEELYARIVATGKAQDPESAGKITGMMLDLPNQEILSLLENDELFKQHFDDASQAYEEFKQAVGEQQAEDGEEEAAAAPAAESTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYQ9</accession>
    <name>A0A1E3NYQ9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sulfatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_65064</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036924.1">
        <property type="nucleotide sequence ID" value="XM_019183854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYQ9"/>
    <dbReference type="STRING" id="683960.A0A1E3NYQ9"/>
    <dbReference type="GeneID" id="30201100"/>
    <dbReference type="OrthoDB" id="3978690at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019637">
        <property type="term" value="P:organophosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16025">
        <property type="entry name" value="PAS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050738">
        <property type="entry name" value="Sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693:SF33">
        <property type="entry name" value="ARYLSULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693">
        <property type="entry name" value="ARYLSULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="5">
        <location>
            <begin position="8"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ57717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="570" mass="64410" checksum="91BACE542E6BB96F" modified="2017-01-18" version="1">MTAEKIRPNFLLIVADDLGFTDISPFGGEIDTPNLAKLARIGQSFTDFHTAPACSPTRSMLLSGTDNHIAGVGQMAEALRTFNEDLKDQPGYEGVLNQRVAALSEILKDEGYYTTISGKWHLGLTEESSPSARGFEKVNALLPGGSNHFKYFPLDPQGETPNNFLPPLFERDGKFFHAGKAKDQIPDDYYSTKYFTDNLLDQLKSKDRDGRPFFSLLTYTAPHWPLQAPEEFIAKYKGVYDKGPAQLRLDRLKRAAELGIIDKDTIPHDVKIKYQRWETLSEENKRREVKIMEVYAAMVDYLDKQIGRVLDELEASGEIDNTFIVFMSDNGAEGAIWEAFPMKTKIVSMVTSNYYNNDFDNIGKKDSFVYYSDEWAQAATAPNFLYKFYSTEGGINCPFIVSYPELINAERKNAGVISNSFLTVMDILPTVLRLADVKHPGKSFHGRAVVEPIGKDFTSVFFDPKHEIHTYEDSKAIGWELMANRAIRRGDYKLNCVPAPYGSGEWELFNVKKDPGETKNLSEELPEIYEQLIHDWAVYVAETGLVEVTTKASFSEAYDELEKVLPRVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ51</accession>
    <name>A0A1E3NZ51_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">coproporphyrinogen oxidase</fullName>
            <ecNumber evidence="4">1.3.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_33982</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aerobic coproporphyrinogen-III oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.3" evidence="4"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037084.1">
        <property type="nucleotide sequence ID" value="XM_019182138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ51"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ51"/>
    <dbReference type="GeneID" id="30199384"/>
    <dbReference type="OrthoDB" id="15318at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00322"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004109">
        <property type="term" value="F:coproporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1500.10:FF:000006">
        <property type="entry name" value="Coproporphyrinogen III oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1500.10">
        <property type="entry name" value="Coproporphyrinogen III oxidase, aerobic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001260">
        <property type="entry name" value="Coprogen_oxidase_aer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036406">
        <property type="entry name" value="Coprogen_oxidase_aer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018375">
        <property type="entry name" value="Coprogen_oxidase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003727">
        <property type="entry name" value="PRK05330.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10755">
        <property type="entry name" value="COPROPORPHYRINOGEN III OXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10755:SF0">
        <property type="entry name" value="OXYGEN-DEPENDENT COPROPORPHYRINOGEN-III OXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01218">
        <property type="entry name" value="Coprogen_oxidas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000166">
        <property type="entry name" value="Coproporphyri_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00073">
        <property type="entry name" value="COPRGNOXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102886">
        <property type="entry name" value="Coproporphyrinogen III oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01021">
        <property type="entry name" value="COPROGEN_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="320" mass="36876" checksum="B6BBD0866E857B63" modified="2017-01-18" version="1">MSFHNSAPNAPIRERMENLIRRKQQEITKGLETLEPTTRFFADSWSRGEKAGGGTSCVIQDGETFEKGGVNISVVYGQLPPAAVARMRADHKNLKAQDDGNVPFYACGLSMVIHPKNPHAPTTHLNYRYFETWNPDGTPQTWWFGGGADLTPSYLYEEDAKLFHQVHKDALDKHDSKLYPRFKKWCDEYFYIKHRQETRGIGGIFFDDFDEKDPEEILKIVEDCFDAFLPSYVPTITKRKDTPFTPEEKNWQQLRRGRYVEFNLVLDRGTQFGLQTPGSRVESILMSLPATASWAYDHHPEKGSREDQLLEVLKNPIEWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ62</accession>
    <name>A0A1E3NZ62_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_80449</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037511.1">
        <property type="nucleotide sequence ID" value="XM_019185064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ62"/>
    <dbReference type="GeneID" id="30202310"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="149" mass="17209" checksum="F9901FBF2DE06719" modified="2017-01-18" version="1">MNNDNSKTRLTANFMRVNMKTQNKSVYGETPGEVLNEIKFYCEQQCSIVEFNRRCKSLRTGDSVNKQSSESFIDSYEQILENYKMVENTTGVKFRRIGTFMRGLSPRYQGFRAHIQNYVNGQDEIANVYGVSSSKLVELFRNSVDDGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZA2</accession>
    <name>A0A1E3NZA2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">OPA3-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_17454</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OPA3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037700.1">
        <property type="nucleotide sequence ID" value="XM_019180743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZA2"/>
    <dbReference type="STRING" id="683960.A0A1E3NZA2"/>
    <dbReference type="GeneID" id="30197989"/>
    <dbReference type="OrthoDB" id="2129069at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010754">
        <property type="entry name" value="OPA3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12499:SF0">
        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12499">
        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07047">
        <property type="entry name" value="OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15653" checksum="4ADA5963C47E444C" modified="2017-01-18" version="1" fragment="single">MSGIGLKLGSLFIRQISKPIANILKAQAKEHDTFKKACISIAQRLHRTDVNLRMKLMGEKGAKVRPLNDKKAIENGANFLSETFVFAVAGSIILFESYRQRQKELTRRETVADDIKTLQYEIQYLKRKLQQYNVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZF2</accession>
    <name>A0A1E3NZF2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Nicotinate-nucleotide pyrophosphorylase [carboxylating]</fullName>
            <ecNumber evidence="5 12">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_43294</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>nicotinate beta-D-ribonucleotide + CO2 + diphosphate = quinolinate + 5-phospho-alpha-D-ribose 1-diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Hexamer formed by 3 homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the NadC/ModD family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="5 12"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037776.1">
        <property type="nucleotide sequence ID" value="XM_019182577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZF2"/>
    <dbReference type="STRING" id="683960.A0A1E3NZF2"/>
    <dbReference type="GeneID" id="30199823"/>
    <dbReference type="OrthoDB" id="10067394at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034213">
        <property type="term" value="P:quinolinate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01572">
        <property type="entry name" value="QPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000090">
        <property type="entry name" value="Nicotinate-nucleotide pyrophosphorylase [carboxylating]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.20:FF:000003">
        <property type="entry name" value="Nicotinate-nucleotide pyrophosphorylase [carboxylating]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="entry name" value="Quinolinate phosphoribosyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179:SF3">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 12" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase N-terminal" evidence="14">
        <location>
            <begin position="43"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase C-terminal" evidence="13">
        <location>
            <begin position="120"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR006250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ58569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="297" mass="32749" checksum="3B14A17618FE040A" modified="2017-01-18" version="1">MSANPNPTFSHLLPLNGEWKQDVTKWLSEDVPSFDYGGYVVGETPETATLWCKQEGVISGIPFSEEVFKQCKVEVEWLVEEGQYLSPNGKLAVAKIRGPARNILLAERTALNLLARASGIATQSFKTISLARKSGYKGIIAGTRKTTPGLRRLEKYSMLVGGCDQHRYDLSSMVMLKDNHIWSTGSITSAVKNARKVCGFAVKIEVECQTEQEADEAIEAGADVIMLDNFEGPDLKIAANNLKARWTGKKSFLLECSGGLRLDNLEGYLTNEIDVYSTSSIHQGVSYVDFSLKIDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZK7</accession>
    <name>A0A1E3NZK7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_64632</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in DNA replication and cell separation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the SDS23 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037705.1">
        <property type="nucleotide sequence ID" value="XM_019183778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZK7"/>
    <dbReference type="STRING" id="683960.A0A1E3NZK7"/>
    <dbReference type="GeneID" id="30201024"/>
    <dbReference type="OrthoDB" id="449052at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001211">
        <property type="term" value="P:negative regulation of isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031139">
        <property type="term" value="P:positive regulation of conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900735">
        <property type="term" value="P:positive regulation of flocculation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902472">
        <property type="term" value="P:regulation of mitotic cytokinesis, division site positioning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000035">
        <property type="entry name" value="Protein SDS23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016711">
        <property type="entry name" value="Ssd23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF36">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018148">
        <property type="entry name" value="UCP018148_CBS_YBR214w"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0129">CBS domain</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="9">
        <location>
            <begin position="190"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="9">
        <location>
            <begin position="266"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="381"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="438"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="452"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR018148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="476" mass="52929" checksum="4A96C7367C02BB45" modified="2017-01-18" version="1">MAPTPSKYQGSPFRQPNSPQPHHPSSRQSSIVEMLSTPPPLEANDNELEQPNALNLSRNPSQSSQSSLNTNSTNQTREHKDWQDIKLSELIESAKLIFIKSDLPVEDAFKLLTTHNLTSLPVEEYDNDINCLTFDYTDLNAYLLLVLGKLAVNEQQFAQNPYYKNPEEIKELITKAKRGQQVPVKFVCQLAPKNPFYKLPETESLSTVMEILGSGVHRIAITNESSTKITGILSQRRLIKYLWDNARRFPSLEPIFQFPISELNIGNSKVISIYGDEPLINALIKMNTERISSIAVVDHHMNLLGNISVTDVKHVTKSSQSPILHKSCLHFISIILNLRGLEDGKDSFPIFHVYPSSSLARTIAKLVATKSHRLWVVRPLNSSSTNSSASTPSGTNSAGTNESNPLIPEHEGRAGKLIGVVSLTDILGLIARKHGKFHVDPNQARKQRRRSSSSSTRSSGSLDQFRRSISGASDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZW7</accession>
    <name>A0A1E3NZW7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Low temperature viability protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_31672</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LTV1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037953.1">
        <property type="nucleotide sequence ID" value="XM_019181865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZW7"/>
    <dbReference type="STRING" id="683960.A0A1E3NZW7"/>
    <dbReference type="GeneID" id="30199111"/>
    <dbReference type="OrthoDB" id="5852896at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071986">
        <property type="term" value="C:Ragulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904669">
        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007307">
        <property type="entry name" value="Ltv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531">
        <property type="entry name" value="LOW-TEMPERATURE VIABILITY PROTEIN LTV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531:SF0">
        <property type="entry name" value="PROTEIN LTV1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04180">
        <property type="entry name" value="LTV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="236"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="373"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="244"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="390"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="408" mass="47383" checksum="D975034DB4065F63" modified="2017-01-18" version="1">MSTRKWIDKKQGKTYKVVHRAHDDPLYHDEDASEHVLVEVQNPNNRKIKTKAQLEEEFKEELEDMRENEGEAALYGITYDDSKYDYMQHLKPIGKSDGVFVEKDGKVQKKKGIVFKDGFELPEDVLPSKKTVTQTYQDQQNIPDEIAGFKPDMNPALREVLEALNDEAYVEEGDDIFQDLLQGGAEEVDEEDFEDQFDEWDLDNYEDEMAQYDEKAFEKEGDEGWEADFRKYKAVSKNKKNDWDSDDEFEDEEDVVPDLPSFDANSKSKSGKGSKRKQRQKKGAMTDTSSFSMSSSANFRNEGLTLLDDRFEKVMKDFEDEFFEEEDEEEPKEFDMKNERADFEGMLDDFLDNYEIEGKRRIVKKNPELDKLKKAADSASKGKLAQRRRKENDDGADPLKGKLGNLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P010</accession>
    <name>A0A1E3P010_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PIH1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_63250</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIH1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037950.1">
        <property type="nucleotide sequence ID" value="XM_019183516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P010"/>
    <dbReference type="STRING" id="683960.A0A1E3P010"/>
    <dbReference type="GeneID" id="30200762"/>
    <dbReference type="OrthoDB" id="5135119at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097255">
        <property type="term" value="C:R2TP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050734">
        <property type="entry name" value="PIH1/Kintoun_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041441">
        <property type="entry name" value="Pih1_CS_Ascomycota"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012981">
        <property type="entry name" value="PIH1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997">
        <property type="entry name" value="PIH1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997:SF0">
        <property type="entry name" value="PIH1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08190">
        <property type="entry name" value="PIH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18482">
        <property type="entry name" value="Pih1_fungal_CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIH1 N-terminal" evidence="2">
        <location>
            <begin position="10"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Pih1 Ascomycota CS" evidence="3">
        <location>
            <begin position="246"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="38189" checksum="339358D70ECBA27D" modified="2017-01-18" version="1">MLEKVPSKEDELLLRPKPSFVIKTKLDVSTQKPKREKGTKVFLNICSDDNVPLPEVGYDPSIIYPLIMDNKWEIPIVTSQERVDKDKKGQLAYVYDCIINTKAMRWVQRDKELREILVEWSLESAELRSGIQLSRESIATPKMVSKGEVQELTILKSELDINRLNKEFERINNMQNEPQSILEAQKIDDEEMEEEQNSIDIFNVNASADQSDSSITKPLIQEITNMSINPPPSKTKSQPQVITKEKLKYSTTFSKIDTNGYKLKIEIKSQNDSSLDYDMHLDKSNNTIILKNLNPKYSTTKDFELPLPTVFQNPSIKSFFVHDQSTLVIFVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P091</accession>
    <name>A0A1E3P091_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 13">Cytochrome c oxidase subunit 6, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">Cytochrome c oxidase polypeptide VI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_79404</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of a catalytic core of 3 subunits and several supernumerary subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion inner membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome c oxidase subunit 5A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038066.1">
        <property type="nucleotide sequence ID" value="XM_019184886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P091"/>
    <dbReference type="STRING" id="683960.A0A1E3P091"/>
    <dbReference type="GeneID" id="30202132"/>
    <dbReference type="OrthoDB" id="5778907at2759"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00923">
        <property type="entry name" value="Cyt_c_Oxidase_Va"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.40:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.40">
        <property type="entry name" value="Cytochrome c oxidase, subunit Va/VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003204">
        <property type="entry name" value="Cyt_c_oxidase_su5A/6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036545">
        <property type="entry name" value="Cyt_c_oxidase_su5A/6_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14200">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14200:SF11">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02284">
        <property type="entry name" value="COX5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48479">
        <property type="entry name" value="Cytochrome c oxidase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0349">Heme</keyword>
    <keyword evidence="8 13" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ58859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="158" mass="18303" checksum="3747F93463107582" modified="2017-01-18" version="1">MFSSSIKQILRTPVVKRSIISTVRPTSTTLRLTTLNRISAVRNYSGAHDEETFEEFTARFEKEFEEAYDLFEVQRVLNNAFSYDLVPSPAVIEKALRAARRVNDFPTAVRVFEGLKYKVENAEQYKAYLDELKTVREELGISLKEDLFPESVNEKHFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0A2</accession>
    <name>A0A1E3P0A2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Spliceosomal protein DIB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_33031</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">Essential role in pre-mRNA splicing. Also essential for entry into mitosis (G2/M progression) as well as for chromosome segregation during mitosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DIM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019182025.1"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="147752at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02954">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Spliceosomal protein DIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004123">
        <property type="entry name" value="Dim1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052:SF5">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEN 4A, 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02966">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="mRNA_splic_U5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="141" mass="16533" checksum="EC7100E4CFD585F8" modified="2017-01-18" version="1">MGSVFLPHLHTGWHVDQAILSEDDRIVVIRFGRDWDKDCMLMDEILYGIAEKVSNFAAIYLCNIDEVPDFNEMYELYDPMTVMFFFRNKHMMCDFGTGDNNKMNFVIDDKQEMIDIIETIYRGARKGRGLVVSPVQYTRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0J0</accession>
    <name>A0A1E3P0J0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_79327</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037987.1">
        <property type="nucleotide sequence ID" value="XM_019184872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0J0"/>
    <dbReference type="STRING" id="683960.A0A1E3P0J0"/>
    <dbReference type="GeneID" id="30202118"/>
    <dbReference type="OrthoDB" id="1099063at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031370">
        <property type="term" value="F:eukaryotic initiation factor 4G binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061752">
        <property type="term" value="F:telomeric repeat-containing RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000805">
        <property type="term" value="P:negative regulation of termination of RNA polymerase II transcription, poly(A)-coupled"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006415">
        <property type="term" value="P:translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12339">
        <property type="entry name" value="RRM2_SRSF1_4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF62">
        <property type="entry name" value="SERINE_ARGININE (SR)-TYPE SHUTTLING MRNA BINDING PROTEIN NPL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="8"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="83"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="192"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="192"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="229"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="259" mass="28672" checksum="07DC87C75F02A4A5" modified="2017-01-18" version="1">MSEPLSETRLFIRPLPFDVREEEIKDFFGPIGEIKEVSIANGFAFVEYENADDAKKAVEELNNKQFIDAPLEIQFSRARKERFRVLVSNIPEGSAWQDLKDFVVDQGFEVTYANVHTRDNDGTGVLEFPTEDQLNDALAKLNGADFKGAALGLERDLNPPPIRSSRGRGGFGFRGGFRGDFGGRGGFRGRGGFGDRGGFRGRGGFGDRGGFRGRGGFGDRGGFRGRGGFRGDRGGFRSRDEGRDFGERSFNRDRSPGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0N4</accession>
    <name>A0A1E3P0N4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_79434</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038095.1">
        <property type="nucleotide sequence ID" value="XM_019184892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0N4"/>
    <dbReference type="STRING" id="683960.A0A1E3P0N4"/>
    <dbReference type="GeneID" id="30202138"/>
    <dbReference type="OrthoDB" id="410651at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042805">
        <property type="term" value="F:actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080135">
        <property type="term" value="P:regulation of cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051510">
        <property type="term" value="P:regulation of unidimensional cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="2">
        <location>
            <begin position="194"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="366"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="512" mass="59313" checksum="94CBAD322D685AE9" modified="2017-01-18" version="1">MRIEFHAEKAFFKTDTIDPFTNLPVYVFDSNYLPNFEDANLDRKTFEQLIVKAFKRIITRIPKHPYVLTCFTSGFKNFNHTNNSWITCLKCYQLLPDELRSHLKKAYIVHESWLVRSMIQVLQNVLRIGFFKTNSINGEKYDRLIYCNNLTELSRHIDITKIRISLDVYLYDSQLEDRLIIPYTTKHNSSDYRKYKEQIFERITTRLLLEGAEFELVFTKPGNQKKLSILNEAIDRGNYLDVSQWDIYVMGSLYLSILRNEIPTLIPIDSIQLPISDDYQYTLSTFQKIIRANNQFDIIYKFFEILCTFLRNSTKTKHDIKTLSKSITPTLSQEKVSLKNNDRIAIGQRFIKNLLDNWDSIVRDITSSSKPVPQTPAPRKVSFHKRVPSIQRTEPKALPDTPNPKRHASTSSMISISSEFSSSSDENIIIETEPIETTKTTSGNELKVLADVTSSLNKSTNGPMINRKKQSKVVSKFSDGYSSIEGQKKVNQLAKLYEERLMGLQIMKDIDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0W7</accession>
    <name>A0A1E3P0W7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_79580</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038252.1">
        <property type="nucleotide sequence ID" value="XM_019184916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0W7"/>
    <dbReference type="STRING" id="683960.A0A1E3P0W7"/>
    <dbReference type="GeneID" id="30202162"/>
    <dbReference type="OrthoDB" id="2382881at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF9">
        <property type="entry name" value="CARRIER PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G06390)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="32"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="130"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="237"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="36053" checksum="2696DC4034543CC1" modified="2017-01-18" version="1">MTKQSNPPPITSTPSSSSQALIILPQSLQPWKPTFLSYTASLGSVAFGYPLDSIKSRMQTHGFNSITKCFLSTLKNEGVRGLFRGISSPIFSIAFSRSINVSVFSIVKPYSSKLSLNLQSNSPILNVLANNLPRSFAAGYCAGFVTSLWACPFEFVKLFSQISFLENKSKIGNIEALRQILNSQGYKGLYTGYKFQFLRDTFGAAVYFSSYESFKLILNSMMLKEDDTWSILRDFSNNWVSIALSGGLSGSLCWLTIFPFDTIKSLQQKALISTIVDKEHKTFETLKLRRNIYRGVSASVLRSFFTSMVFFSTYEYLVKIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0Z9</accession>
    <name>A0A1E3P0Z9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Regulatory protein MIG1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_32733</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in glucose repression of glucose metabolism genes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the creA/MIG C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038210.1">
        <property type="nucleotide sequence ID" value="XM_019182002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0Z9"/>
    <dbReference type="STRING" id="683960.A0A1E3P0Z9"/>
    <dbReference type="GeneID" id="30199248"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000433">
        <property type="term" value="P:carbon catabolite repression of transcription from RNA polymerase II promoter by glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000089">
        <property type="entry name" value="DNA-binding protein creA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000072">
        <property type="entry name" value="zinc finger protein 143 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051007">
        <property type="entry name" value="creA/MIG_C2H2-ZnF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428">
        <property type="entry name" value="REGULATORY PROTEIN MIG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428:SF1">
        <property type="entry name" value="REGULATORY PROTEIN MIG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ59003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="91" mass="10682" checksum="130E55C8DD1F28BF" modified="2017-01-18" version="1" fragment="single">MTESNNPGQEPTTVKDDTKTKQPKKQANKSDKQELPRPYKCPICDKAFHRLEHQTRHIRTHTGEKPHSCTYPGCFKKFSRSDELTRHLRIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P103</accession>
    <name>A0A1E3P103_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_69024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037804.1">
        <property type="nucleotide sequence ID" value="XM_019184154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P103"/>
    <dbReference type="GeneID" id="30201400"/>
    <dbReference type="OrthoDB" id="3982967at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06293">
        <property type="entry name" value="Kdo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="721" mass="83658" checksum="88644678B62A50C6" modified="2017-01-18" version="1">MSIEKLKDSYVRDYFQLILHNVLEELKNNTIWNINYHNFYKDVPKVTKYPTDVTKVDRLLLVKTIQTLGMRYNYEAHSYQDTAALAKILLILPFMGLEIDGYNPESPSTAQEDSKKSNTSPSTGSNNSLAHQIKSSNIKLSAKDTKENEVDSWLHERLETIDQDLMRNIQNACSADHKEVWRNLNYIWNCAGRPIEYDNREDDITLALYSCLIDPVTRLFNIVYNMRLNARVNKPLKDAVEFESPKFGEHIRSDLGVWSNSNGKAGIHLVLETKEPFLSPFFNSKERGFDEKYRDFYCQLLSYLMISPTVRKGLLTDGVSMRFTHFNQIDIVNGNMNSVQVDESSTNNFIDEKSKTIDMWKIYSQDSTPQDRVTAAKIVMCILYDSCCGLINGSNSQLERSDEDILTISSRCEFDTSTSPLLLQTSSGSLDVNISLSKDQYSLPTTAARRNQFTNQPLNLKSVPEAEIMVKIDNLGECIQEYDIDSPIEVYKMSAENYYLNFKPKNPVQGIPIQDEQSLKDGNVILKLYDEEKIEKYFKEEGMSKYYTPTNEDGGSPLFDEERYNSDFKAYEQRILNRYLNEIEAYYRIQNFNETCLHPKHKINSPSLIGYGKFKQHSSGPFIYYMAISEIPFVDRKPHNYDELFFKGCNEIKKLHSLGIIHRDIMRCNICFDEQTSSIWIFDFNRCLLFDPSQEIEQKLLNKDFIDLHDYEFSYADIYEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1K9</accession>
    <name>A0A1E3P1K9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">SWI5-dependent HO expression protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SHE3</name>
        <name evidence="13" type="ORF">WICANDRAFT_94628</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Required for the delivery of cortical endoplasmic reticulum into the emerging bud.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the SHE3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038548.1">
        <property type="nucleotide sequence ID" value="XM_019186454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1K9"/>
    <dbReference type="STRING" id="683960.A0A1E3P1K9"/>
    <dbReference type="GeneID" id="30203700"/>
    <dbReference type="OrthoDB" id="6088208at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048309">
        <property type="term" value="P:endoplasmic reticulum inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031398">
        <property type="entry name" value="She3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17078">
        <property type="entry name" value="SHE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="315"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="378"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="74"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="315"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="346"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="385"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="400"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="428"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="478"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="510"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="536"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="562"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="602"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="655" mass="75125" checksum="8B2DF10E3C174F19" modified="2017-01-18" version="1">MNNKENEPAMDQELSTTGIEDHKLARPESETSSPTKSSPTRTPLNASNMISPSKDIRSSVISPRKATNSSTRVIESLHKEIDELKSEVLKLKSSNEELKKSNELVKKRRDQLMEQLSNSKHENETVNSLLQRKQRRINDLESQLNEITSSNDDLKFKFQSLETRSEKLRQSEASSVAEYERMKIAYETIVTSQKEYRDHYSKEIATLKERLNEFVKNKEDQINRNITLIAKSDSTLGRSVKSITNKANDLEKLYEDRNLKLEEKIDNIASTHRNNNDQTAFLLQTSKDLFNEIADKLEIDKEELLKTYLEQDSSRVDPFKEEEEVKSTPTQQPIQIKKRNERKTTSSRSTSSSSSTNGRIPSVEERVLSLDKELNKSIPLKDRITPNTSNVSRSKSMRGSRNSSHNNSQVNNSNNSSNPLFSRITPNDAKRPTRNYEDRDSNNQRNTSRNDSRRTSRNFDEHNNKNRSTNTSRRSSRVFDSNQQVTETGNSENKEAGAGESNADGAEAPKRKRRRRRRRRGRKGEHQQADENNNNSEDDDDEEDDNEEGGDDQDVSIIESDVETKDLEKKETIDTEVLNEIKEESEEKFDELTLQDQKESVEIEDTEDTEKELEPQTGDTQPILKDGIAEESEPKINASKSTQLEDTLEEKDNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1V6</accession>
    <name>A0A1E3P1V6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_85020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038676.1">
        <property type="nucleotide sequence ID" value="XM_019185827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1V6"/>
    <dbReference type="GeneID" id="30203073"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="155"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="291" mass="33821" checksum="03741FD0E9DF180C" modified="2017-01-18" version="1">MILEQSPKTIEKTDVFKATEERDLMRLTVEEKNRQIGELTEQIEVTVQENIDNYEKILKAEELIKDKLESLNDKKIQLAQSKDKLSSFKNSDPSLPKLQDLFRNVSILSENVDESVLNSIEGMQRLIDEHLGKIHSIQKQIMDAEIALSKEQLRINNEKLKYDELFKQNESLTKQIESIKQSLNDENNSTPEKLKLQNDEEKLKTLMKIWLKITNIEDFKIDDVDGSLKFRFKDYPDHDCLISVDKNHKIAKIHYELVGDEVLNEMVIKSNKAKDPVESVVMNLYKTISDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1W3</accession>
    <name>A0A1E3P1W3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4 15">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_55151</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Endoplasmic reticulum membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4 15"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038104.1">
        <property type="nucleotide sequence ID" value="XM_019182755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1W3"/>
    <dbReference type="STRING" id="683960.A0A1E3P1W3"/>
    <dbReference type="GeneID" id="30200001"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF50">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="199"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="598"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="628"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="656"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="685"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="336"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ58897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="735" mass="84142" checksum="3B8300380C74D43F" modified="2017-01-18" version="1">MAKKKVITPPGAKKVQINNTTTTSSSSELPPNYKKGIKRPYFSTIIPEKDILDAKSTKFDYIIVTLLTILGFYIRIIGINKPNSIIFDEIHIVRHVNDYLNGTFFLDITPPFSKMFYAFIARVLGYKGDSTNLTTPGQSFQNTDFPFIGLRTVSAILGAGVVPLTFFTLRSTGSRPLIAGFGSLLVVFENSFIVSSRLFMADSISLFLLALGIFAFKKFQLTSPFTKDWARFLVLTGISLGLGISTKWIGFSTIVWVGIITVQQLWYLFGDIEVSNCKLVKHVSSRISLLLISPIVIYLGVFAIHILLLNQTTPDSSLLTTHFQRTMSGNDIYDLPKYVTFGSTVTLRHSESLGGFLHSHPYPYKSGSHNQQVTVFDYKDYNNEWIIEPTGNVDENDKRLRGDTVVKLRHKNTGALLRVDNFKPPISEQEYDFEVSCFGDYEYQGNDTDVFLLKIDDDSKSKDFVKSSETQFRLWNKKKRCTVISHDLKLPEWGFGQQEVLCIESPTVARTLFTIETVKYPQDVDFTGNPELLEKVEEIKPSFWSKFLELNTKMMRIFQSIKPKNNNSPDPSSWPMLPSGLRYFTRPKSAIYFIGNPIIWWLSIMLMPSFIFIRIVDFFRIDNNTNSIRYKAHTLTFLVGWFLHFIPYVINEGEFFISYYIPSLYFGILIIGSTFEYIYFKNAKLGNTLIVITILFAYYFYSTLTPITYGSNWDVTSCQQSKLLDTWNYSCDIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P209</accession>
    <name>A0A1E3P209_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sulphur transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_31716</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038708.1">
        <property type="nucleotide sequence ID" value="XM_019181871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P209"/>
    <dbReference type="GeneID" id="30199117"/>
    <dbReference type="OrthoDB" id="10254418at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046513">
        <property type="entry name" value="DUF6691"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp_TsuA/YedE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YEDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574:SF1">
        <property type="entry name" value="SULPHUR TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20398">
        <property type="entry name" value="DUF6691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="278"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="35199" checksum="AE7A692D3E084C6C" modified="2017-01-18" version="1">MFSIIEASVGALFIQLASTNYYWQLGSTIGFSSAIYNTIFEKATKNIPLLTGVLCSSLFIKEYAPSFLPDFPTSNLITNFGIDKLVLFSIAGALVGLGTKIGAGCTSGHMIGGLSRLRVRSMAAVATFSAAAIGLNKLLDLGLHSAVEPNYTLVLSWGFFKENKLITGLLIAGFIQVYGLLPYLSQKAKGFYKCLLGNYIRFLSGFLFGIGLHVSGMVNVTKTIGFLSILDWKKFDPSLAMIIVFAVIPNVIIWRNIKKPLLTGNFDLAMSTEIPPRFLIGNVLFGLGWGILGICPGPGLANSIHFRDVNIWLISFLTGHGIGGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P222</accession>
    <name>A0A1E3P222_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutaminase</fullName>
            <ecNumber evidence="2">3.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_31482</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaminase PdxT/SNO family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="2"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038718.1">
        <property type="nucleotide sequence ID" value="XM_019181838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P222"/>
    <dbReference type="STRING" id="683960.A0A1E3P222"/>
    <dbReference type="GeneID" id="30199084"/>
    <dbReference type="OrthoDB" id="2039at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903600">
        <property type="term" value="C:glutaminase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008614">
        <property type="term" value="P:pyridoxine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01749">
        <property type="entry name" value="GATase1_PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01615">
        <property type="entry name" value="PdxT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002161">
        <property type="entry name" value="PdxT/SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021196">
        <property type="entry name" value="PdxT/SNO_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03800">
        <property type="entry name" value="PLP_synth_Pdx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01174">
        <property type="entry name" value="SNO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005639">
        <property type="entry name" value="Glut_amidoT_SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01236">
        <property type="entry name" value="PDXT_SNO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51130">
        <property type="entry name" value="PDXT_SNO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="78"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="170"/>
            <end position="171"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="248" mass="27883" checksum="66D7A1A38C3E752D" modified="2017-01-18" version="1">MTQNYPILNLARIILFIKDMRNQKKYTVGVLALQGAFKEHIHLLNQATNNYDSYEISYIEVRNQQQLSTCDALIVPGGESTSMSLIAERSGMLEPLANYALLKKPIWGTCAGLIFLSKQVINGSPGQKLIGALDIQVKRNAFGRQLDSFEQNLDFTSFIPGVNDFPSIFIRAPVVSKILKTSSSDEIITMKETIKKVNESPVKILYQLDNGLIVAVRQGNILGTSFHPELSDDCRFHKWFIDEFVLQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P254</accession>
    <name>A0A1E3P254_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor 1A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Eukaryotic translation initiation factor 4C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_85031</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 9">Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the eIF-1A family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ59485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:ribosomal small subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:formation of translation initiation ternary complex"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:formation of translation preinitiation complex"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="S1_IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000071">
        <property type="entry name" value="Eukaryotic translation initiation factor 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00216">
        <property type="entry name" value="aIF_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018104">
        <property type="entry name" value="TIF_eIF-1A_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00523">
        <property type="entry name" value="eIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="EIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01262">
        <property type="entry name" value="IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1-like" evidence="11">
        <location>
            <begin position="22"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="104"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="124"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025502"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00032507"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00067293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ59485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="152" mass="17320" checksum="BFB5A5405C937EB0" modified="2017-01-18" version="1">MPKSKGKGGKNRRRGKNDNEGQKRELTYKDEGQEYAQITKMLGNGRVTASCFDGVERMAHIRGKLRKKVWMGQGDIILVSLRDFQDDQCDVIQKYNSDEARALKSQGELPENAKINETDTFGHEEEEDVNFEFGAADDDDEEDDEELDIDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2E5</accession>
    <name>A0A1E3P2E5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_19493</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019180827.1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33875">
        <property type="entry name" value="OS09G0542200 PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF13462">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="2"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="186" mass="21512" checksum="D97DBFE4727CD8E4" modified="2017-01-18" version="1" fragment="single">PPNTIEVYLDYACPFSGKLYKNWYDNLFPLLETKYKGKFQFIFRNYVQVWHPTSNLLHEASLVFAQLDPSNFLKFSYVLFELIEEFYDTATAHLSRNEIYEKIYELAVAPHFSTTVDKSKFLKSLVIKKSEDHHSNSGNKATNDLKYFTKVGRQNGIHVTPTVLINGFKDGSIESSTPIDQVQEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2L4</accession>
    <name>A0A1E3P2L4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Autophagy-related protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_94836</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in cytoplasm to vacuole transport (Cvt), pexophagy, mitophagy and nucleophagy. Recruits mitochondria for their selective degradation via autophagy (mitophagy) during starvation. Works as scaffold proteins that recruit ATG proteins to the pre-autophagosome (PAS), the site of vesicle/autophagosome formation. Required for the Cvt vesicles completion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Preautophagosomal structure membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Vacuole membrane</location>
            <topology evidence="1 8">Peripheral membrane protein</topology>
        </subcellularLocation>
        <text evidence="8">During pexophagy, accumulates in the vacuolar membrane region, where the peroxisomes contact the vacuole.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ATG11 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038651.1">
        <property type="nucleotide sequence ID" value="XM_019186463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2L4"/>
    <dbReference type="STRING" id="683960.A0A1E3P2L4"/>
    <dbReference type="GeneID" id="30203709"/>
    <dbReference type="OrthoDB" id="447953at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903599">
        <property type="term" value="P:positive regulation of autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034517">
        <property type="term" value="P:ribophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040040">
        <property type="entry name" value="ATG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019460">
        <property type="entry name" value="Atg11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045326">
        <property type="entry name" value="ATG17-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222:SF1">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10377">
        <property type="entry name" value="ATG11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04108">
        <property type="entry name" value="ATG17_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="Autophagy protein ATG17-like" evidence="11">
        <location>
            <begin position="129"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy-related protein 11 C-terminal" evidence="12">
        <location>
            <begin position="1072"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="599"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="641"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1078"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="599"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="616"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1192" mass="136972" checksum="0CA1D9EB939059E5" modified="2017-01-18" version="1">MNDNDIQVFNAHSGESIKINRYYFVTLEELKRFCSNQFNIPSNQLFLISPFGIKIKRTTSLEETSEIYVFDKNLFTTNKSDEYIRSYISSQYAENHDNLIQPINSPLLDVNLEKVASSKNTRQMIGLLTTNLGWVAAIESDSALFHKKIIAMGEKIRILFKALTVATQYIDSYCLDVKKNYDSSIDFVISIQNHSLNLTWKDHYKRLKYIPLVDGEEKPLSSLLDQRELENQARECIKLNESINSELMGYKSKIESSKKSRGEVEKDIGVLESETKKVVEHLESEEDLKELKLLSDKVQADTKHLLNKTKNKLDEIDIELILETLQTHKGDFVSKIFKLSLKLYKIFLNWNDLGAKLQVDLTKQLQRLSGAQSEIISVKEALKNISGKIEQVQNLESSLSHTVDLPLLYGLYIVEDIRRNEWLLEMKSLSSKTVENFASLREKEIKLRSQWVRNFGTILKLLDYNIASFNSRNIANIDLNINEDKSLIQSGKFTKEDALNYVDKLKASGVNQDTINVITKAINDLPLRKSRIKLNDQDTTDANSSDQVIKGYKARIKKLESLLHQEQFKHFSHWPSGASGESKFPGLSRYSLVFDKSQNRSQLRPASGTGQLIGDTKFESQTRDRSLSPTADTKIIEPEELASLRNEKTDLLNKMRKMKDTINIINDELKSIKKDFSSKESENQILLKQLDLVRDENLANLAKLQLQITSKEKEKSQLDELTSKQKQELSNLEKLLKERDGIVDKLKSSHATEVDSLKKELTKKDAEFKEYESKNDIRDELEALRSELEAQKAENSSLKDQLDVRERDLEDIEEKSKKQNEELEKLKKQGLSVKDLESQRDKLVNVNIDLNKDIDKWKSNYNTLSSMKNDLLENMSNRESEFAKEKKSQQEEIESLKMKLEDMDRLQDESHKGDEGHNGNQTILQLVIIINSLIIKSRDLSEILYNNYGLFCSTLRSIGLLAVKLESGEINIIRVKGLRKLGDNNDLSNTIDMSNGAKPDLEKDVEKYLAWTELPDSKLIADLSSTQSEDDQSKSLDLVCETLIENYNLTNFEEKYLSFVNEVTNLLPSFKASVSKRFREVENLAKRELKENKRFKENERQKISIRDFKVDDLVLFLPTRSNIGSSNEDEARSNNWAAFNDMGDMKFLLKNDLKIPPNKQWIIGRISSIEEVENDSKEFLITAEEAVLNSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2X2</accession>
    <name>A0A1E3P2X2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="9">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_30222</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 16">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="9"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039043.1">
        <property type="nucleotide sequence ID" value="XM_019181702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2X2"/>
    <dbReference type="STRING" id="683960.A0A1E3P2X2"/>
    <dbReference type="GeneID" id="30198948"/>
    <dbReference type="OrthoDB" id="1470711at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17932">
        <property type="entry name" value="DEXQc_UvrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.486.10">
        <property type="entry name" value="PCRA, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070:SF2">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE SRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="12">
        <location>
            <begin position="16"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="13">
        <location>
            <begin position="295"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ59836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="644" mass="73091" checksum="9FA4FF699906B46D" modified="2017-01-18" version="1" fragment="single">MLYSGSKDVLQRILSGLNDAQRAAVTSSPNGQLQIIAGPGTGKTKVLTSRVAYLMIEHNIRPEHIIVTTFTKKAANEMKERLSQMLEGTNIQLNKLVIGTFHSICMRILMQYGYVINLKGFKIAADRDWDEVMKSLIDPITDDIADIRDLKLRKQISKLKSKGIYPDDYSLQKDYNKELHIIFKLFQEKLESSGLLDFDDLLVKTSQLLSKRNCVSKIKHVLIDEFQDTNTIQLQLMVKFAMGVPGNCNNITVVGDPDQSIYRFRDAVALNFELMRKKYPGCEIIKLNENYRSTSDILDFSEAVMRQQNSRTSKNLSSQFSHTFPAVHQSFGSREEEAVNIAQEIKFLKLLPKLFQNSDFAILIRAAYQSRALERALIEKKIPYRIVKGRAFWERKEVEQIVDFLRLVAYDDDKAAFLRTLKSCTSGIGPVTIKKIDAVIDEESKKGFTAISALYKIARGDFKGVGSSKNKDQIQLFLEMVEKARNFVANDNYSPNQLSLLFEFLKSQEPLASLIKEEERAQNIEEVKSQLVGFVPTEVEPINSEDSAPLNEDTFLQSFLSSIQLYSVDDKEEDNEDGVVCISTIHSAKGLEWPVVFIPGLTEGLLPASFAKKDENEEEAIDEERRIFYVSTTRAKQLLYVTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2Z0</accession>
    <name>A0A1E3P2Z0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Cysteine proteinase 1, mitochondrial</fullName>
            <ecNumber evidence="2 9">3.4.22.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_31371</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Has aminopeptidase activity, shortening substrate peptides sequentially by 1 amino acid. Has bleomycin hydrolase activity, which can protect the cell from the toxic effects of bleomycin. Has homocysteine-thiolactonase activity, protecting the cell against homocysteine toxicity.</text>
    </comment>
    <comment type="function">
        <text evidence="7">The normal physiological role of the enzyme is unknown, but it is not essential for the viability of yeast cells. Has aminopeptidase activity, shortening substrate peptides sequentially by 1 amino acid. Has bleomycin hydrolase activity, which can protect the cell from the toxic effects of bleomycin. Has homocysteine-thiolactonase activity, protecting the cell against homocysteine toxicity. Acts as a repressor in the GAL4 regulatory system, but this does not require either the peptidase or nucleic acid-binding activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.</text>
            <dbReference type="EC" id="3.4.22.40"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homohexamer. Binds to nucleic acids. Binds single-stranded DNA and RNA with higher affinity than double-stranded DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.40" evidence="2 9"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038920.1">
        <property type="nucleotide sequence ID" value="XM_019181828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2Z0"/>
    <dbReference type="STRING" id="683960.A0A1E3P2Z0"/>
    <dbReference type="GeneID" id="30199074"/>
    <dbReference type="OrthoDB" id="2666448at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070005">
        <property type="term" value="F:cysteine-type aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043418">
        <property type="term" value="P:homocysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00585">
        <property type="entry name" value="Peptidase_C1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004134">
        <property type="entry name" value="Peptidase_C1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363:SF2">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03051">
        <property type="entry name" value="Peptidase_C1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005700">
        <property type="entry name" value="PepC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0788">Thiol protease</keyword>
    <feature type="active site" evidence="10">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="416"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005700-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="496" mass="56460" checksum="051B191A2C50E89D" modified="2017-01-18" version="1">MGANTSRESTVRDNRDIEKLSYRDDESYEEVLNEKFASVSLKPQVNESKTKSAEAISLESIDKWEASLLSDKKNLLTINALAHNPIVNVIKNHKQITKDSVDIWNTQASLEGSPITSQNSSGRCWIFASTNVFRTFYQKKFDIESFELSQAYLFFWDKLEKSNYLLNNIIETADEKLESRVVQTILANGLSDGGQWDMIVNLVNKYGLVPKSVYNDDYHASNSAALNYVLVNKVREYALELRRLINDEKVSKEEVTHVKDKQIHEIYNIIALLLGPPPKADEEFTWEYKDKSGKAHSIKNSPLDFASLVDLQINETFSLINDPRNDYDKLYTVDKLNNVVGGKPIDYVNVKSIVQKKVIIDSLKDNQPVFFGCDVGKFQDNGVLDTEAFDYELAFNTKLNLTKEQRLKVGSSAMTHAMVITAVHVIDGEIQRYKIQNSWGETAGNKGYFVATDKWFDEYVYQVVTSTKYAPKYLTEIWKKGDYSVLPIYDPMGALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2Z2</accession>
    <name>A0A1E3P2Z2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peptide transporter PTR2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_83892</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039063.1">
        <property type="nucleotide sequence ID" value="XM_019185613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2Z2"/>
    <dbReference type="GeneID" id="30202859"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071916">
        <property type="term" value="F:dipeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000085">
        <property type="entry name" value="MFS peptide transporter Ptr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01023">
        <property type="entry name" value="PTR2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="495"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="526"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="556"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="643" mass="71203" checksum="B4393C8592C608F8" modified="2017-01-18" version="1">MSFNIKEKISSRLRSNDEEVSSINSSEAKLDKFDELAEYNEKYPEPTAEDYATLPKVLGHAPYATYLICLVEFAERASYYGVKDRLNNFIQLRLPTGGNGAGAPPRGTQQNAGALGLGLQTASAITLLLTFLAYLTPLYGGYISDKKLGRMKTIWYGVWIGAISHIILIIAAIPSVIQGGHALAPTVISIICLAFGTGFIKPNLLPLLFDQYPHQRDIVETREDGKKVILGREATLERMTLVFYWAINIGAFMSLATSYSAKDVGFWLAYLIPGIVYIIMIPTLMLLSPRLKKETPNGISIIEETLKVIKFSLKGGWIKRLRTGQFWEFAKPSNIAARGEVQAVEAVNKKGKKKVSWTDQFVQDVKVTVDACKIFLFFVIYNINDGGIGGIQNSQANTMTTNGVPNDLINNFNPLTIIVLIPILDYGIYPILRKFKIEFRPVYRIFFGFILAALSSVAGAIIQWQVYETSPCGYHATDCEIGTGVSPISVWAETVLYILGASSECFANTAAYEIAYTRAPDNMKGLVMALFLFTTSISAAISEACTSALIDPYLVWPFVATAIAGVLAAFWFLWLYRDLHKVMEAERIAKEEKVREEYAEYLAEKGVNVKSSDSIDRIADQQTSIKEETDGLYAIGSALSHRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2Z3</accession>
    <name>A0A1E3P2Z3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eisosome protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_62445</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EIS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039068.1">
        <property type="nucleotide sequence ID" value="XM_019183368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2Z3"/>
    <dbReference type="STRING" id="683960.A0A1E3P2Z3"/>
    <dbReference type="GeneID" id="30200614"/>
    <dbReference type="OrthoDB" id="4070583at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024527">
        <property type="entry name" value="Eisosome1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28298">
        <property type="entry name" value="EISOSOME PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28298:SF1">
        <property type="entry name" value="EISOSOME PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12757">
        <property type="entry name" value="Eisosome1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="377"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="605"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="690"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="498"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="605"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="690"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="753" mass="83850" checksum="43CADCA6CADE73B1" modified="2017-01-18" version="1">MSLANSQISNKSKHSSVYQTNGKPLSPEALYKAKLKYGIYNNPTTQSIGVPSSSAASDTAALLANSTDLSIEPYKRQLAADAQTAALFARKDTAPQKWSKDYIDHDAASAAISAKSLKTQIIKKPSDNSSNAAAASVLSKDSKSLASSNLASLYEFDAVRNGTKNSHASLNIEKLSKASKERADTLINSRINPTRDSSRLGLASKETNNDHLEKFAAVGALASQNFKPPQDPNLEAKAIYKNSLVDPKVLAKARLNADRTLTGIDKHLGEQDLFLNPEFNKIAIQIAQSHSTKRIASSDKINLGGGLFMTQEELDSIASKIVNPVLEDISAKAKKQREKDLEIQKKKEEQIKLHRQVKLQQQAKKLEEKRLKEEAKLNRRKEMEQEKEKQKQAKLELEKAKKEELSKHQDILTAKQKEEERLKKELLAKKQAEEERIQDESTKAEKQRSKELQDAKEERDLKLAPILDQLKIETDKLAVLNEEKQAIQDITDSQVKNTENARNLLIASQTELTNAENQLELIKQDIAKSNDESETLIKESELKKQEAEVALKKSNEIEAEALLKQAEIDKEKAIVENERLKLELELENYKIEILEQEKKIAEILPPTKKEEVKSKDVKPETATKGATATESSPKETSELPTTIVTGTGTVDISKNFQSQDPSTKTIVDASGAITPASKQHQHVKSLVDEVVPKDSQKDVKDEDDHELKKTFTGFSQGSGVEKENLESTPEDVVVDDADLSKEDKHKSFFKEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P328</accession>
    <name>A0A1E3P328_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cystathionine gamma-lyase</fullName>
            <ecNumber evidence="4">4.4.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Gamma-cystathionase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_78354</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.1" evidence="4"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038925.1">
        <property type="nucleotide sequence ID" value="XM_019184709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P328"/>
    <dbReference type="STRING" id="683960.A0A1E3P328"/>
    <dbReference type="GeneID" id="30201955"/>
    <dbReference type="OrthoDB" id="3512640at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004123">
        <property type="term" value="F:cystathionine gamma-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047804">
        <property type="term" value="F:cysteine-S-conjugate beta-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019343">
        <property type="term" value="P:cysteine biosynthetic process via cystathionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904828">
        <property type="term" value="P:positive regulation of hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000043">
        <property type="entry name" value="Cystathionine gamma-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000008">
        <property type="entry name" value="Cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF15">
        <property type="entry name" value="CYSTATHIONINE GAMMA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="5 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="389" mass="41459" checksum="5668479F45AF7B1C" modified="2017-01-18" version="1">MSSYNFSTRAVHVGSSPDPTTGAVIPAISLSTTFAQTAPGKPIGEFEYSRSSNPNRANLEQVLASLENGKYGLAFSSGSAVTATILQSLPIGSHAISVGDVYGGTHRYFTKVANTHGITTEFAHDIVNDLPNLIKKDTKLVWIESPSNPTLSITDIAKVKEVISKSSNPDIVLVVDNTFLSPYISNPLNFGADIVVHSATKFINGHSDVVLGVAVTNSEDIYQKLTFLQNALGAIPSPFDSWLAHRGVKTLALRVKQAATSAQKIAEFLSTSPNVVAVNYPGLPTHKGYEIVQKQHRDSLGGGVISFRVKGGQDAASKFTQNTKLFTLAESLGGIESLLEVPAIMTHGGIPVAEREKSGVFDDLVRLSVGIEDTEDLIQDIKQALEAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P349</accession>
    <name>A0A1E3P349_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosomal protein L43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_30207</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL43 family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ59773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_019181700.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3P349"/>
    <dbReference type="GeneID" id="30198946"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.30:FF:000002">
        <property type="entry name" value="60S ribosomal protein L37a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_eL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050522">
        <property type="entry name" value="Ribosomal_protein_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
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        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003058">
        <property type="entry name" value="PRK03976.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ59773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="91" mass="10107" checksum="D6346F3B2DCDB69A" modified="2017-01-18" version="1" fragment="single">TKRTKKVGITGKFGVRYGSSLRRQTKKLEIQQHARYDCSFCGKKTVKREATGIWNCHSCRKTVAGGAYTVSTAAAATVRSTIRRLRELAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3K8</accession>
    <name>A0A1E3P3K8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_92188</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039283.1">
        <property type="nucleotide sequence ID" value="XM_019186351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3K8"/>
    <dbReference type="STRING" id="683960.A0A1E3P3K8"/>
    <dbReference type="GeneID" id="30203597"/>
    <dbReference type="OrthoDB" id="2275718at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034063">
        <property type="term" value="P:stress granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045117">
        <property type="entry name" value="ATXN2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009604">
        <property type="entry name" value="LsmAD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12854">
        <property type="entry name" value="ATAXIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12854:SF7">
        <property type="entry name" value="ATAXIN-2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06741">
        <property type="entry name" value="LsmAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01272">
        <property type="entry name" value="LsmAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="2">
        <location>
            <begin position="75"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="503"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="678"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="453"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="503"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="521"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="678"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="728"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="742"/>
            <end position="758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="783" mass="86984" checksum="D8610F194E392BDF" modified="2017-01-18" version="1">MKQTGANSTGSTGRNNSFEKKHNRSTSKNSNNGNSKKLESNSSSASNSSPSNGNAVTSQLNLLNDEELQAHLNDRLLYLIVNSIGSQVNILTNSGTIVKGVLQNVDPKSMKIIVKNDGEGQFTSFNYEDLLEFELENVDLFNNSYVSNKASSGFRTDQDIGFKNSNAPRQEMEKWVPDAALDVSLDQLTFDENDPNGNVKSWDQFETNEVKFGVKSEFDEELYTTKINKNDPNYQKNLEKATKLAAEIESQSYNGNIHLAEERGLKFDDSGVDEEDKYSGVDRSVSVDAKGDALFQSLINKDSKNFQFGDEKSKYVPPSQRSKIQNIDPSIVSATNNGTKPTPAPKQSLPSKPQTQAPAPVSEKIKNDTSVPPKPPVKEVQKPETSSKQEKKSVPEHLKKLNTINEINALKEFSQTFKIPSKFPQDLLPILAKDKSKQEEIIKKQDNSKKDVASSTTSSSNVASPKPKTATPKLSSVAPTKKVYDPAKSQPFKLNPKAAAFTPTTANTTPSTASNVSTPVFNKNSPRLASNQPTPNNYNRKRFNATPASFFGNDRVPSLEKQSKKLYNDFNFFKGCLAEFEKKKSGENAELNKDLKFFLEKPFFTPPTWTKDESEKPYKSFFPALEAIKFQRMMPTPLPPNPMQQFQPIPMMQQPGPFFYPPQPFIPQHQNFIQLSPQAQSPALHSGSISPSNSPFQQQRPMYGQHHHHNYHPAASMPYQPQRYAPNTPIPPPTGYNPNYPPGSSGIPTPGIPMTPINQGPPPPQFQPPSAGSHHQNRGYKHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3V1</accession>
    <name>A0A1E3P3V1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_78776</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039366.1">
        <property type="nucleotide sequence ID" value="XM_019184787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3V1"/>
    <dbReference type="GeneID" id="30202033"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="542" mass="62032" checksum="C34471B4DD902538" modified="2017-01-18" version="1">MEVVPKLIEMLNMVLNIFFHSPGPVEGSTLGTPSPDAPVEVFYQIINSWVNMFYASFKLAISAISPYIATAAIIINHSFKLLSTGGNIIGKTHQTFSILPLSIRELTVVTLIGSWCLFRFGVKSRKSNKQVKVDVHVHNHNKQITQVANNSSTLFDRSFFQFNRTNQAQSRRKPIRYFIPTITLNGDRATVWKKVSTATKHKQFNNDSFIDAVFRAAGEGSNAQVLNELEDREFQSFHQAKDFILSFSVHTKEFNQHREDAVNQLLTHEPVYTVEEFKLFLVKLSEAYYLSYLDSTRLLSALLTIGCLRTLKEKALLEQLRAKLTSLDINEFAEQLLSQFNELQLKQHCISRRPKAHQVQQPEPFAKEQATVKEEANTDQPRGSYSLENRNDGNGNVITQSSTPSSVTNEPERIQHHRIYHTRLTVFSKEVVRRVKDRYQHKENLSRQSLDTEIICSDGKVINEKLAEQLWEAIVADDTMSTTSEGTIENDVVSSNLQSKIAESEIYKDEEAFQGYESIDKEWQSNIQNENYLPQLWINSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P421</accession>
    <name>A0A1E3P421_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Shikimate dehydrogenase substrate binding N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_62729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039372.1">
        <property type="nucleotide sequence ID" value="XM_019183426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P421"/>
    <dbReference type="STRING" id="683960.A0A1E3P421"/>
    <dbReference type="GeneID" id="30200672"/>
    <dbReference type="OrthoDB" id="204377at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019632">
        <property type="term" value="P:shikimate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022893">
        <property type="entry name" value="Shikimate_DH_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089:SF1">
        <property type="entry name" value="BIFUNCTIONAL 3-DEHYDROQUINATE DEHYDRATASE_SHIKIMATE DEHYDROGENASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089">
        <property type="entry name" value="SHIKIMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shikimate dehydrogenase substrate binding N-terminal" evidence="1">
        <location>
            <begin position="24"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="315" mass="35332" checksum="97648FFF5C259C67" modified="2017-01-18" version="1">MTISKTLLAPNFEEQKSQPHTFYLFGEHLSKSKAPFLHNALFKSLGYDEWDYKIYETSYISEISHKINSEKLFGSAVTMPNKVKAVELVDLLDDIGKGCGSINTIYTRTDPNNGRALKIGTNTDTVGIKESFVQNFPIETEQLSSSPGLVYGGGGACRSAIFALYKLLNVPKVYIINRYESEVEEVKVSMKLNGFEGEIIHVKTAEQAREMDPPKLVVLTVPNFEPQTTEEKIARNTLDVFINQKSPGIVLEMCYHPVIETQLFHNFLNAGWKVISGVEAMIYQGIAQQVLWTGIPIDKIPTKKIKEQVYSSINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P465</accession>
    <name>A0A1E3P465_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycogen [starch] synthase</fullName>
            <ecNumber evidence="9">2.4.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_29895</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Glycogen synthase participates in the glycogen biosynthetic process along with glycogenin and glycogen branching enzyme. Extends the primer composed of a few glucose units formed by glycogenin by adding new glucose units to it. In this context, glycogen synthase transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;4)-alpha-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18549"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9587"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:18550"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyltransferase 3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.11" evidence="9"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039486.1">
        <property type="nucleotide sequence ID" value="XM_019181664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P465"/>
    <dbReference type="STRING" id="683960.A0A1E3P465"/>
    <dbReference type="GeneID" id="30198910"/>
    <dbReference type="OrthoDB" id="6335297at2759"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004373">
        <property type="term" value="F:alpha-1,4-glucan glucosyltransferase (UDP-glucose donor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000014">
        <property type="entry name" value="Glycogen [starch] synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000045">
        <property type="entry name" value="Glycogen [starch] synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008631">
        <property type="entry name" value="Glycogen_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10176:SF3">
        <property type="entry name" value="GLYCOGEN [STARCH] SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10176">
        <property type="entry name" value="GLYCOGEN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05693">
        <property type="entry name" value="Glycogen_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6 9" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 11" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="629"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="684" mass="77697" checksum="EA0DABDEF3D8B11F" modified="2017-01-18" version="1">MTRDIKNHLLFEVATEVANRVGGIYSVLKSKAPITSSEYKDRYTLIGPLNYDSAAVEVEELKFTDPAIKATLDSMGSRGIRYLYGRWLIEGAPRVLLFDVGSAGHHLDEWKTDLWNISGIPTPNGDTETNNAILLGYLVAWFLGELVYHDRERAVIAHFHEWLAGVALPLCRKRRIDVTTIFTTHATLLGRYLCAGSVDFYNNLANFDVDAEAGKRGIYHRYCIERAAAHSADVFTTVSLITAYESEHLLKRKPDGVLPNGLNVVKFQAVHEFQNLHAIKKEKINEFIKGHFYGHYDFDLENTLYFFISGRYEYRNKGVDFFIESLARLNHRLKESGSKTTVVAFIIMPAQTHSYTVETLKGQAVIKALENTVKEVQNSIGHRLLEHCSRYQQHGKEVPDLDDLISSSDRVLLKRRVFALKRDGLPPIVTHNMSDDANDPILNQIRRVGLFNNPSDRVKIIFHPEFLNANNPILPLDYDEFVRGCHLGVFPSYYEPWGYTPAECTVMGVPSITTNLSGFGGYMENLIENSSDYGIYIVDRRMKSVDESIDQLTNQMFDFCQKTRRQRINQRNRTERLSDLLDWKRMGLEYIKARNLALRRAYPNLFTDSNGGSGSESLFNSKIKISRPLSVPGSPRDRNNIMTPGDLGSLQDAAVTDDYFQLATNEDEDQYGQYPLSLGEAEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4D5</accession>
    <name>A0A1E3P4D5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_78389</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038959.1">
        <property type="nucleotide sequence ID" value="XM_019184716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4D5"/>
    <dbReference type="STRING" id="683960.A0A1E3P4D5"/>
    <dbReference type="GeneID" id="30201962"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="70"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="215" mass="24632" checksum="9E6C44172E444ECD" modified="2017-01-18" version="1">MSLIPSPPDLKPTIIQMQNSSENITPSEESVDPKFVPPNNSQPSEQPKRKRRRSSASLISQNEIEKRRREHKTAHSIIEKKRRIRMNREFEALKFIVPACRNNLSTSNNNGEGMYKLTILQATVDYIKYLHQVINIQQDEINKNQSYPNISDQDLDFAKIDVDTDSYRNLDIEYDFNKLFKDFQSQASSSSSSSSNSRSSSDSASSKKNRNQTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4E6</accession>
    <name>A0A1E3P4E6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP-2 complex subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_30314</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <location evidence="1">Coated pit</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039558.1">
        <property type="nucleotide sequence ID" value="XM_019181717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4E6"/>
    <dbReference type="STRING" id="683960.A0A1E3P4E6"/>
    <dbReference type="GeneID" id="30198963"/>
    <dbReference type="OrthoDB" id="28053at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050840">
        <property type="entry name" value="Adaptor_Complx_Large_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017104">
        <property type="entry name" value="AP2_complex_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003164">
        <property type="entry name" value="Clathrin_a-adaptin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009028">
        <property type="entry name" value="Coatomer/calthrin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22780">
        <property type="entry name" value="ADAPTIN, ALPHA/GAMMA/EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02296">
        <property type="entry name" value="Alpha_adaptin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037091">
        <property type="entry name" value="AP2_complex_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55711">
        <property type="entry name" value="Subdomain of clathrin and coatomer appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0168">Coated pit</keyword>
    <keyword evidence="3 7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin adaptor alpha/beta/gamma-adaptin appendage Ig-like subdomain" evidence="9">
        <location>
            <begin position="681"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="48"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037091-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ60351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="922" mass="104271" checksum="F7917FC7135EEE47" modified="2017-01-18" version="1">MKGLQQFIADLRTLNAKEEQDKRIKSEIVNIQKQFSSGSKLTGYQRKKYISKLIYIYLTSSCNTSNLDFQFGHNQILELLSSKVFSEKFIGYLSISLLISSNNKSESEADFLNLIINSLKNDLNSHDIEANLLALQCIATLGNETWANFLAEDVFQILRSPTSSPLLKKRSSLALLKLVKASPEVLARHNPWIPRILALCDDSDLGVVLSVLPLIQFIAKEIDFAQAQQIIPTLAKKLNLLIMESNNVPDDYFFGEIANPWLIVRICGLLETLIPDVQHLNIDLNTLRTLRACVSKTIEFTTKKTNDLAVRNAHNSVLFSVIGLASHLDPSPEALSSSIDAITKLLDSNETNTRYLALNSLIALCSQGGALSLQTSRKHLLKLFHLLRDQDVSICRKSLDLIYTLTDSESIEFVVDELLKTLKGSDHVLKSEIAIKVSVLSEKFAKNPLWFVTTMLELIDHVGLTLNESIWERVVQIVVNNENLHKFTCAELMRYIMKPNFAEPTVKIAAYLLGEFGYTIKDEFPIHQQFDILSTIYFYVSNSTRAMLLTTFLKFQKVEIEEKQLKSKLIKFLRIEINSVDSELQQRAVEYLAMIQKLNSEKGKVLFNIVFDESPVFNSRQNPLLLRLGNSTELNKSALVKSTDNLTQKLTTKAPNPFKQNSPVKKSAGAPLTPNWDKGFYRIFEFNQGVFFENSLVKVILRINKNNNKKDLLSFTLTFTNKSPRPLSSFITTLTEYKTVKPNIIVNLLELPDTDIDIDHKTSSIIEVLLRSPFSMEDIPNLRIQFNSGGGFNNIRLKLPIFLTKYITSTALPFPQFLQHWKQIDSLGESGESIKQIYSDRAGDKQWLLRFVEKLGFAIVEQQSELFIFGAGILHTSSSGNFGTLMKLKLIDQGTFEIRIRATSPNLGELIANSIVSYFEHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4F0</accession>
    <name>A0A1E3P4F0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4112 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_30141</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039430.1">
        <property type="nucleotide sequence ID" value="XM_019181691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4F0"/>
    <dbReference type="STRING" id="683960.A0A1E3P4F0"/>
    <dbReference type="GeneID" id="30198937"/>
    <dbReference type="OrthoDB" id="2103474at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025187">
        <property type="entry name" value="DUF4112"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35519">
        <property type="entry name" value="MEMBRANE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35519:SF2">
        <property type="entry name" value="PH DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13430">
        <property type="entry name" value="DUF4112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="61"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="21850" checksum="4A51D6478DBEBA55" modified="2017-01-18" version="1" fragment="single">KEDPFFEEIPNEELKFYQRHGARRKKALPLGLSHNDQKILKSVRRRAYHLDLSLSCCGFRFGWAGIIGIIPWIGDFFAAYLALLLLKKAQQIDGGLPSYIQTQMSANIAFDFGIGLIPIVGDIINIAYKANSRNCLLLESYLTHKYNKKHKHETSAETEGRISDIPESQPGPNNAKPKIPDYQKQPPHPPPRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4F7</accession>
    <name>A0A1E3P4F7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_92448</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039440.1">
        <property type="nucleotide sequence ID" value="XM_019186365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4F7"/>
    <dbReference type="GeneID" id="30203611"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="54" mass="6222" checksum="278793FCC5F81080" modified="2017-01-18" version="1" fragment="single">LSIISYEDPVVPAECAPLRLAPFEKKYCEAHDQIAGFHGSKRYNFSVFLKYRCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P506</accession>
    <name>A0A1E3P506_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_27900</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039753.1">
        <property type="nucleotide sequence ID" value="XM_019181423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P506"/>
    <dbReference type="STRING" id="683960.A0A1E3P506"/>
    <dbReference type="GeneID" id="30198669"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010255">
        <property type="term" value="P:glucose mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000115">
        <property type="entry name" value="High-affinity glucose transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF17">
        <property type="entry name" value="HEXOSE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="289"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="419"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="456"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="12">
        <location>
            <begin position="29"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="520"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="532"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ60546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="548" mass="60081" checksum="33DE1EBA1DD67A8A" modified="2017-01-18" version="1">MGLEQNWLVRRYINIDEKSEGSTTMAIFTGLFVAFGGVLFGYDTGTISGILAMPYVLKHFPEDEPGVDGGEGVFTSSEHSLIVSILSAGTFFGALLAPLISDTLGRRWTLIISTTIVFNLGIILQTAATNINLLVAGRVFAGLGVGLLSACVPLYQAETVPKWIRGAITSSYQFAITIGLLLAAIFNQATHKIDSTASYRIPIALQFLWSIILTVGMLFLPETPRFYVSKGRNEEAAKSLARLRRLPADHPALVDELGEIIANFEFESVHGKSGWLDCFRTSNHQLKRLITGIFLQAFQQLTGINFIFYFGTTFFKNSGIKNEFLIQLATNIVNVGMTVPGILLVELLDRRTLLLGGAAGMCVSEMLIAIIGVSTKSDAANKTMVAFTCIFISFFASTWGCVAWVVVGELFPLRTRAKSVAMSTASNWLWNFAIAYATPYLVDDAPGSARLQSKVFFIWGGCNFLCFFFAYFFVYETKGLSLESVDELYDTVPSAWKSRGFVPTDHKFTRELGHRTGGSVVASEDEELGGEKQQTTNVERLPTGQESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5D3</accession>
    <name>A0A1E3P5D3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cell wall protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_78659</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039244.1">
        <property type="nucleotide sequence ID" value="XM_019184767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5D3"/>
    <dbReference type="STRING" id="683960.A0A1E3P5D3"/>
    <dbReference type="GeneID" id="30202013"/>
    <dbReference type="OrthoDB" id="4068457at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133689" description="Cell wall protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="22302" checksum="D7629E8C7717ACE0" modified="2017-01-18" version="1">MKLSFLLSFLSTSAIVLANTQNFTLKCFAPNTVLEDANLHVDEDNKIYIANVSETATGQLLNNGSLAIGDTIMGIGKNYLSLEADSTSFEYVNSWSIENGYLKLYGSDFHAVPSGMDGIYVLGSINAAAGRDDVIPIAIKAEDSSGATIADFNPESGSSSSVLSVLGSQTFDHSSASMTALKSSVSSSNLGSENFVKLFGALGSGLVALIMMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5E9</accession>
    <name>A0A1E3P5E9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein ARV</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_28954</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediator of sterol homeostasis involved in sterol uptake, trafficking and distribution into membranes.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Regulates also the sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Golgi apparatus membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ARV1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039878.1">
        <property type="nucleotide sequence ID" value="XM_019181560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5E9"/>
    <dbReference type="STRING" id="683960.A0A1E3P5E9"/>
    <dbReference type="GeneID" id="30198806"/>
    <dbReference type="OrthoDB" id="2192830at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035621">
        <property type="term" value="P:ER to Golgi ceramide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097036">
        <property type="term" value="P:regulation of plasma membrane sterol distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035376">
        <property type="term" value="P:sterol import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007290">
        <property type="entry name" value="Arv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467">
        <property type="entry name" value="ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467:SF0">
        <property type="entry name" value="PROTEIN ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04161">
        <property type="entry name" value="Arv1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 10" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="300" mass="34657" checksum="DE4239FCDA6DB718" modified="2017-01-18" version="1">MICVECNAPVDSLYVKYANDYIKLTDCPSCSKVADKYVEFDNVILFIDTLLLKPQAYRHLVYNLMGNAPNDLKQSSQRSLKGWFLKNKKVNRIRLLMILFKIYLTWAYEEKNYNFEESINPFLIMNSILKKNAVTQYLYFSLKCISDDVITHTFIPFFMFNVVGWNPNGYVSKDSHNKRYYKMALSLTILISGATNLFPILMLIWPYNNITITNIISSVADLSLIEALRIVTDCSIPSAISVFLLNTLIKFIVTRMLLIVILTKGNESLAYSIANHEYANFVQRIMGLRQILERSLKPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P624</accession>
    <name>A0A1E3P624_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatidylethanolamine-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_62861</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039504.1">
        <property type="nucleotide sequence ID" value="XM_019183450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P624"/>
    <dbReference type="STRING" id="683960.A0A1E3P624"/>
    <dbReference type="GeneID" id="30200696"/>
    <dbReference type="OrthoDB" id="2506647at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF82">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133684" description="Phosphatidylethanolamine-binding protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="27980" checksum="9D68856377FE66CE" modified="2017-01-18" version="1">MVHLLSTVIWLLILSPFGFSIQPETVQNLYQILPKFKFKSIGIKETLEESDILKDYLYHWKPPGELNIEFFEIYSKPVTYGNYISPLDCLEPPVEIEFVSFFSLSPNFVNELYTLVLNGVDSDGFETCQAIWVNIPFNDENYEIIEHVNGKESFKTKLDLRGGDDLMKYYGPRPKSGSGIHQYVFVLFRQPHGWFPPHKLKFRENWGSKHPAHHGVVSWANYYALKPLGINFFTSSNENLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P646</accession>
    <name>A0A1E3P646_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Membrane protein TMS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_83084</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039957.1">
        <property type="nucleotide sequence ID" value="XM_019185486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P646"/>
    <dbReference type="STRING" id="683960.A0A1E3P646"/>
    <dbReference type="GeneID" id="30202732"/>
    <dbReference type="OrthoDB" id="5963193at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005016">
        <property type="entry name" value="TDE1/TMS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383">
        <property type="entry name" value="SERINE INCORPORATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383:SF9">
        <property type="entry name" value="SERINE INCORPORATOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03348">
        <property type="entry name" value="Serinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="440"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="469" mass="52253" checksum="22107EC72C507C4E" modified="2017-01-18" version="1">MGAIVSLPFQIAGTWVASCCGSMCFSLVSKSFKSIGSSFSTRLSYAFLFLLNTILSWVMLTDFARSKLEKLSKFQCEGVECGFFAVHRINFSLGIFHVILAAMLVGVHSTTNPRSKIQNNFWAPKILLWLVFIVVSFLIPDKFYIGWSTYVSVFCGALFLLIGLILLVDFAHEWAETCIEHVENEDEYSSVWKTILISGTSLMYLGSLVMTILIYVFFCKSGCSMNQAAATVNLILSIIITVLSLNRTIQEYNPNCGLAQAAIVSVYCTYLTLSACASEPDDKQCNPLIRSRGTRTASVVLGAIFTFITIAYTTTRAAANSAFNGNNGGGAITINYDDPVSTEPIISTQPSARNEMRLQAIREAVAVGTLPESALHDQSWLYDDEDDEDEEKVSTKYNYALFHIIFFFATQWIAVLLTMNVQQDDFGDFVPVGRTYFYSWVKIVSAWICYLIYGWSLVAPVLMPERFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P650</accession>
    <name>A0A1E3P650_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UEV domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_23560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040123.1">
        <property type="nucleotide sequence ID" value="XM_019180994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P650"/>
    <dbReference type="STRING" id="683960.A0A1E3P650"/>
    <dbReference type="GeneID" id="30198240"/>
    <dbReference type="OrthoDB" id="306304at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11685">
        <property type="entry name" value="UEV_TSG101-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052070">
        <property type="entry name" value="ESCRT-I_UEV_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008883">
        <property type="entry name" value="UEV_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23306:SF3">
        <property type="entry name" value="TUMOR SUPPRESSOR PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23306">
        <property type="entry name" value="TUMOR SUSCEPTIBILITY GENE 101 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05743">
        <property type="entry name" value="UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51322">
        <property type="entry name" value="UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UEV" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="89" mass="10070" checksum="F34ADE6EFB4A1F12" modified="2017-01-18" version="1" fragment="single">LQSYPSLKPRTRVYTSETGESQLLLCLYGSLPSPIGGRVYKIPIELWIPHEYPIAAPFVYVVPTEKMTLQPGNHVDNSGRCYSPYLANW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6F5</accession>
    <name>A0A1E3P6F5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">RNA cytidine acetyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">18S rRNA cytosine acetyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NAT10</name>
        <name evidence="17" type="ORF">WICANDRAFT_89433</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">RNA cytidine acetyltransferase with specificity toward both 18S rRNA and tRNAs. Catalyzes the formation of N(4)-acetylcytidine (ac4C) in 18S rRNA. Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis. Catalyzes the formation of ac4C in serine and leucine tRNAs. Requires the tRNA-binding adapter protein TAN1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>a cytidine in 18S rRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in 18S rRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51424"/>
            <dbReference type="Rhea" id="RHEA-COMP:13575"/>
            <dbReference type="Rhea" id="RHEA-COMP:13576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a cytidine in tRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in tRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53876"/>
            <dbReference type="Rhea" id="RHEA-COMP:13670"/>
            <dbReference type="Rhea" id="RHEA-COMP:13671"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with TAN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
            <location evidence="1 11">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the RNA cytidine acetyltransferase family. NAT10 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="11"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6F5"/>
    <dbReference type="STRING" id="683960.A0A1E3P6F5"/>
    <dbReference type="OrthoDB" id="10067491at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990883">
        <property type="term" value="F:18S rRNA cytidine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051392">
        <property type="term" value="F:tRNA cytidine N4-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016556">
        <property type="term" value="P:mRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904812">
        <property type="term" value="P:rRNA acetylation involved in maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051391">
        <property type="term" value="P:tRNA acetylation"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="RNA cytidine acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="tRNA(Met) cytidine acetyltransferase TmcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03211">
        <property type="entry name" value="RNA_acetyltr_Nat10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033688">
        <property type="entry name" value="NAT10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007807">
        <property type="entry name" value="TcmA/NAT10_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032672">
        <property type="entry name" value="TmcA/NAT10/Kre33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013562">
        <property type="entry name" value="TmcA/NAT10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027992">
        <property type="entry name" value="tRNA_bind_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925">
        <property type="entry name" value="N-ACETYLTRANSFERASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925:SF5">
        <property type="entry name" value="RNA CYTIDINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13718">
        <property type="entry name" value="GNAT_acetyltr_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05127">
        <property type="entry name" value="NAT10_TcmA_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08351">
        <property type="entry name" value="TmcA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13725">
        <property type="entry name" value="tRNA_bind_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TmcA/NAT10 N-terminal" evidence="14">
        <location>
            <begin position="10"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="TcmA/NAT10 helicase" evidence="13">
        <location>
            <begin position="281"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="15">
        <location>
            <begin position="534"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="domain" description="Possible tRNA binding" evidence="16">
        <location>
            <begin position="770"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="435"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1010"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="439"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1017"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1034"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="635"/>
            <end position="637"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="642"/>
            <end position="648"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="736"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00052133"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00068357"/>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_03211"/>
        </source>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05127"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF08351"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF13718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF13725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ60532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1043" mass="117653" checksum="1B5589ADE2D2CC16" modified="2017-01-18" version="1">MGKKAIDARIPALIRNGVQEKERSFFIIVGDKARNQLPNLHYLMMSADLKMNKSVLWAYKNKLLGFTSHRKKREAKIKKDIKRGIREANEQDPFESFISNQNIRYVYYKETEKILGNTYGMCILQDFEALTPNLLARTIETVEGGGIVVLLLKSLTSLKQLYTMTMDIHKRYRNDNHGDVIARFNERFILSLGSCENCLVVDDELNVLPLSGGKHVKALPPKDEDELSPKAQELKALKSSLEDVQPAGALVSLAKTVNQAQAILTFVDAISEKTLRSTVALTAGRGRGKSAALGISISAAVSHGYSNIFVTSPSPENLKTLFEFIFKGFDALGYQEHLDYDIIQSTNPAFNKAIVRVDIKRDHRQTIQYISPSDSHVLGQAELVIIDEAAAIPLPVVKKLLGPYLVFMASTINGYEGTGRSLSLKLIQQLREQSVTSGRNDDTEVVSRDSKNSNESEVLGRTLREVTLDEPIRYAPGDPVEKWLNKLLCLDASLSKNLKFASRGTPHPSQCNLFIVNRDTLFSYHPVSENFLQKMMALYVASHYKNSPNDLQLMSDAPAHQLFVLLPPIDESSGRIPDPLCVVQVALEGEISKDMVRRELGRGKRSDGDLIPWLMSQQFQDEDFAGLSGARVVRIATNPDYSSMGYGSRALELLRDYYEGKFTNISESSQEKDYTIKRVSDKELENTDLKKDDVKLRDAKTLPPLLLKLSEKSPHYLDYLGVSYGLTSKLHKFWKNNKFTPVYLRQTANELTGEHSCIMLSPLENRESKWLFEFAKDFHKRFLWLMSYDFKKFTAVQALNVIESAKVNDSQEVEQLTRSELDKLFTPFDLKRLESYSNNLLDFHVIVDLLPVIAGLFFSGRINEVKLSSVQSAIFLAIGLQHKSMDDISKELNLPDNQTIAMFSKIIRKVSTYFRGLVSRSIEDTMPELKDETVAEMDGEEPITRFEEIEDTMEEELTADGKEAVKALREKQRELINSLNLDKYNIDDTNVELNEKDLSKAAVSGKVVALKGKAPKRKQESAEDVYKQEMGSLKKAKKGKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6I1</accession>
    <name>A0A1E3P6I1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RFX-type winged-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_90191</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040117.1">
        <property type="nucleotide sequence ID" value="XM_019186218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6I1"/>
    <dbReference type="STRING" id="683960.A0A1E3P6I1"/>
    <dbReference type="GeneID" id="30203464"/>
    <dbReference type="OrthoDB" id="10056949at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000422">
        <property type="entry name" value="DNA-binding protein RFX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003150">
        <property type="entry name" value="DNA-bd_RFX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039779">
        <property type="entry name" value="RFX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057321">
        <property type="entry name" value="RFX1-4/6/8-like_BCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619">
        <property type="entry name" value="RFX TRANSCRIPTION FACTOR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619:SF5">
        <property type="entry name" value="TRANSCRIPTION FACTOR RFX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25340">
        <property type="entry name" value="BCD_RFX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02257">
        <property type="entry name" value="RFX_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51526">
        <property type="entry name" value="RFX_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RFX-type winged-helix" evidence="3">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="471"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="471"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="524" mass="58345" checksum="4192FA14B92467D1" modified="2017-01-18" version="1">MAITVIQRCESKNSAVVPRNRVYARYVGICADNGLKPLSPASFGKLVRVVFPNLTTRRLGMRGQSKYHYCGIKLLGDNNQPSPSVSSASTPLHNPSFDSSFLPGTPYESNSPNSFHSQASTPLPSNSTASTHVTVISSFINDHVAPDLKFVPDLLQSINNQNTDLDSPLMLPNLKPFLPPSTDLDIADTLYGLYKAHCTSVFESLRYMQLKKLFSLLSSFHGTLTAPVLKLYVSSSLHPWVIASDSVMYKAIIKMLANLALQEIPTHVLQQLKQVAQNYTEKLSISIQHLPVKLVVSKLKLGKEFCQLISRLIRVAETAQSANKVLSHDFDRDLMEKDWIKYVDIDLIASKELPCEGDNLKKAIEILKVKVPKLLKNQDNSKELIINEWANFIAELPQQFKEVPPRLFLLCISALLTSALREISLAGGAGFGAWWVVRCWVDEWVGWCAELGGFVSHQPFDITVEERRSSISDKEKVNGKQDNNKANEESEVPVDLLDGQFGENREVLNVSEEGKESNGEPEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6M5</accession>
    <name>A0A1E3P6M5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit E N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_61635</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040276.1">
        <property type="nucleotide sequence ID" value="XM_019183224.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6M5"/>
    <dbReference type="STRING" id="683960.A0A1E3P6M5"/>
    <dbReference type="GeneID" id="30200470"/>
    <dbReference type="OrthoDB" id="417252at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016650">
        <property type="entry name" value="eIF3e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019010">
        <property type="entry name" value="eIF3e_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10317">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09440">
        <property type="entry name" value="eIF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01186">
        <property type="entry name" value="eIF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Eukaryotic translation initiation factor 3 subunit E N-terminal" evidence="4">
        <location>
            <begin position="16"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="161" mass="18977" checksum="DF369B9219FED906" modified="2017-01-18" version="1" fragment="single">MSELGAAELEIARKYDLTKKVIPFLDRHLIYPILESLRSEDLYDDKAITQLTFDLFKETNMISFVKEQWKTLNPNAQVPKELEEKEAKVDDIFNKLNNETKETLDILNLPEVQDHLKQDKQFNREYLEKNHGITESKINALYEFGQFQYNRGDYVMASDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6Y7</accession>
    <name>A0A1E3P6Y7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Biogenesis of lysosome-related organelles complex 1 subunit KXD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">KxDL homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_61703</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the biogenesis of lysosome-related organelles complex-1 (BLOC-1) involved in endosomal cargo sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the KXD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040340.1">
        <property type="nucleotide sequence ID" value="XM_019183234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6Y7"/>
    <dbReference type="GeneID" id="30200480"/>
    <dbReference type="OrthoDB" id="4089816at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051390">
        <property type="entry name" value="BLOC-1_subunit_KXD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019371">
        <property type="entry name" value="KxDL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37787">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT KXD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37787:SF1">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT KXD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10241">
        <property type="entry name" value="KxDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="KxDL" evidence="10">
        <location>
            <begin position="58"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="75"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ61133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="176" mass="20238" checksum="B4BE51D48781DE36" modified="2017-01-18" version="1">MATRHRRQPSINSQTFAVQAPDVSDQDDEDMSTDSYQDSLEDESANREFDPVEYLTDSLNSALASMEMNRSIVIQAQTSGALKGKEMELEQLQKEAQERLLQFQSRFKEGLKTLKQVTKDLKWTQQHVNSLKKHVEAKHPIEYSQSKDKVLSRAINIDEITNSKDKSNDDDEEIYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7D5</accession>
    <name>A0A1E3P7D5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein mS41</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein FYV4, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_17117</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS41 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040532.1">
        <property type="nucleotide sequence ID" value="XM_019180726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7D5"/>
    <dbReference type="STRING" id="683960.A0A1E3P7D5"/>
    <dbReference type="GeneID" id="30197972"/>
    <dbReference type="OrthoDB" id="18595at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039603">
        <property type="entry name" value="Ribosomal_mS41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019083">
        <property type="entry name" value="SAM_Ribosomal_mS41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28235">
        <property type="entry name" value="PROTEIN FYV4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28235:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09597">
        <property type="entry name" value="SAM_Ribosomal_mS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01238">
        <property type="entry name" value="IGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small ribosomal subunit protein mS41 SAM" evidence="6">
        <location>
            <begin position="22"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="110" mass="12835" checksum="785C8A2673485505" modified="2017-01-18" version="1" fragment="single">ATKAANPNNINYPLPTSEIPNAEAFLQKIGRNCIEHKEHFPNWHLLFNTTSRQMKEKGIDAQTRKYLLSQLEKYRNGEKILEIKKGKKSYFGSEYKRKETVAKMHAAERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7H8</accession>
    <name>A0A1E3P7H8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Condensin complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_27867</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the CND1 (condensin subunit 1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040391.1">
        <property type="nucleotide sequence ID" value="XM_019181421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7H8"/>
    <dbReference type="STRING" id="683960.A0A1E3P7H8"/>
    <dbReference type="GeneID" id="30198667"/>
    <dbReference type="OrthoDB" id="436262at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043007">
        <property type="term" value="P:maintenance of rDNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010032">
        <property type="term" value="P:meiotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051307">
        <property type="term" value="P:meiotic chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903342">
        <property type="term" value="P:negative regulation of meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044804">
        <property type="term" value="P:nucleophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070550">
        <property type="term" value="P:rDNA chromatin condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007130">
        <property type="term" value="P:synaptonemal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070058">
        <property type="term" value="P:tRNA gene clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000272">
        <property type="entry name" value="Condensin complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026971">
        <property type="entry name" value="CND1/NCAPD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032682">
        <property type="entry name" value="Cnd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007673">
        <property type="entry name" value="Condensin_cplx_su1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024324">
        <property type="entry name" value="Condensin_cplx_su1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222">
        <property type="entry name" value="CONDENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222:SF2">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12717">
        <property type="entry name" value="Cnd1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12922">
        <property type="entry name" value="Cnd1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017127">
        <property type="entry name" value="Condensin_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="6 10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Condensin complex subunit 1 N-terminal" evidence="13">
        <location>
            <begin position="65"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Condensin complex subunit 1 C-terminal" evidence="12">
        <location>
            <begin position="333"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Condensin complex subunit 1 C-terminal" evidence="12">
        <location>
            <begin position="915"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="794"/>
            <end position="823"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR017127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF12922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ61184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1099" mass="124228" checksum="1A443FF3318195F6" modified="2017-01-18" version="1">MDFNLSESLTAFQEADKDSFDISNADNLLDQVTGSLAVSLEAISNSSIYESLEELVHAYPKLNKKEQKQVIYLVTSSLHNQAENSSNIIENGETELFVAHKQYLEAYAYLAHCLLTYLGLEHIPSSNTNSKKVTNQALEVFKNNCTQLENIMDAICAVFKLHLSKLFTTTPEKDLFVSSFTRPIYILMENEPRVKVVGVKMYMFKVIAMAVKFHGHASAAQSAIVQNLTYFSHLNSMMAELLQILNDQYDHFQLTEDVLREVSNKEFNSNDNNGPKQISLFIVKVSELIPRILLKQMTLVSQLLNNSSYTLRCAVVEACGNIIIEISKDEDELERYKNQVSGLLDLLIERFLDQNPYVRTKAIQSILKLCEVDAKFTKYRQSFVEIAVRSLEDKSSLVRRNAVKLMSRLVLTHPFGALHGTQLKLSVWEKRLADAEKELESLEPADTTQAVIERNLEQDSDEEEELPSMIEKESNSNAVFKIKLTIQYYNDAIKFIKLIQHGVEIASQLLYSKNKNEVIETMDFFVLADAYSIENVDTGIRRMLHLVWMKSNNDEGTNVATHLVECYKNLFLLAPDDSSELEAAALIAKNLISLTYSATVPELASLEKLLGLMYEASLINETVVKVLWQIYSFQSQNFSKKQRRGAIIVLGMLALSDNDISMKGIDALLNVGLGEKGKEDLVLAKYSCIALNRVIPQDKKHDTFKVPREKEAIDKLANVLIEYNSNSEYYSVAEQAINTIFNIASRPEDVATEIIKQKTKLTFGSSHENESQVVSLSQLLFIVGHISIKVIVHLEALEAQFKKKKIEAEITKAKGDKNEEEANELEMIGGTSEDDFADAVAFIKEKELLYGEHSLLSKFGPLVKEICSNNLKYDNKILQRSAVLCMEKLMCVSSKYCEDNLALLITIMERSEDPIIRSNAVLGLGDMAVCFNNLVDENTDFLYRRLHDDDLMVKRTCLMTITFLILAGQVKVKGQLSQMAKCLEDPDQGISDMCKLFFTELATKDNAIYNGFIDIFSGLSNDENLNKDAMKRIIKFLLSFVEKERHQKQLSDKLVQRITKVENEKQWNDVAFVLGALPIKSDVITQILETGYTMVAART</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7K7</accession>
    <name>A0A1E3P7K7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_104310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040411.1">
        <property type="nucleotide sequence ID" value="XM_019180411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7K7"/>
    <dbReference type="STRING" id="683960.A0A1E3P7K7"/>
    <dbReference type="GeneID" id="30197657"/>
    <dbReference type="OrthoDB" id="3980800at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012491">
        <property type="entry name" value="Red1/Rec10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07964">
        <property type="entry name" value="Red1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="724"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="778"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="727"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="788"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="922" mass="103143" checksum="25A0C6A7BDC21EA6" modified="2017-01-18" version="1">MVKESIQYYITILEDLSQLCSNGKLGEFAKIITAEKRKKFIAFISDGANVVNDKFRLSFNRFLDSIHNKINPKIFEIILKIGVNFMKDDDSFWFHLINILDYGATFDQYELLDLLIEQILKEDENSWKATCLLCIMIQDYPSLRSPCIKSLSKVHEELLAVLSHVKNFTTSSYLVELVSQLEDDALVSLIFKQNPELYEKFPENAYANDPTCLKFLQLTKFDPSIFKFKSFSFGTGLKSGASYCQLTSKYLITLVEHNDFAAPIQFDLSNIRNLQSVGNQARFNLARAAKKALINIDSSESTVIVLTFDSEKTCKGFMSKYQDFAGQITLSTPRKISVVQDFIALSGGFGDMDYDEDIYDGPSQNDEIIEPSAMNNEVSNSGIIDETESLFGSPINTTKDTSVSKKLGRAVSVIVSESVDKGSKMVNPQEPDLFSDDGYQQVPDSLDQAFETFHNPPVDRGPSDVVVESNQPEDSKPAKATQKSDIWDFESDPDVTLNNPQATSTVNKKYRSKAMKCLTNQINSIDKKRTGKKNEPKKKTEVKSKVNDKLEKFNKSGVDPVKDIPSSLDSIDQHGGSFLPLTSPMASAHERARTRALSKALINSEHEAPGVTENKNMGKVETNVGKRIEKKIAKKIDKETEKKPVKRGRTRAKDPEVESDVEYIEKKPSLKKGKVTTSKAVKKVVATKAAPRVTRGKKKQSEAVEVEPVTEIKLVNALPSKPKAQDTFKTVNKSSKVNAPEKSDKSMSAIFAKPDAPKGVAFDKNHPVMESTRLIDLKDKDKTQTDTHASTPAAQTHRPQTNLEQHFSLMSSAFTAPIAADTILSEAYTNTLQRQIFESITSFSNQLVKKIHIINDEINKKVMTDLTSKYETLFNELRDSFQSDVDEMCGFITDVKGLLNLPEKELTDYIKQKKFGTIASKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7M8</accession>
    <name>A0A1E3P7M8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-type proton ATPase subunit G</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_78071</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the V-ATPase G subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040648.1">
        <property type="nucleotide sequence ID" value="XM_019184656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7M8"/>
    <dbReference type="STRING" id="683960.A0A1E3P7M8"/>
    <dbReference type="GeneID" id="30201902"/>
    <dbReference type="OrthoDB" id="3980648at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2950:FF:000001">
        <property type="entry name" value="V-type proton ATPase subunit G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005124">
        <property type="entry name" value="V-ATPase_G"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01147">
        <property type="entry name" value="V_ATP_synt_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12713:SF11">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12713">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03179">
        <property type="entry name" value="V-ATPase_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="115" mass="12751" checksum="C274FBC09EB80C5E" modified="2017-01-18" version="1">MSNQSGIATLLRAEKEAHEIVSKSRAYRTSRLKAAKTDASSEIAAYKQKKEQELKQYEAEHSGLNETADKEAEAQVKEELEKIQKTASEKQAEVVKLLINAVTKPTPELHINASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7T6</accession>
    <name>A0A1E3P7T6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-dependent helicase IRC3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_28919</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040587.1">
        <property type="nucleotide sequence ID" value="XM_019181553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7T6"/>
    <dbReference type="STRING" id="683960.A0A1E3P7T6"/>
    <dbReference type="GeneID" id="30198799"/>
    <dbReference type="OrthoDB" id="16911at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036121">
        <property type="term" value="F:double-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061749">
        <property type="term" value="F:forked DNA-dependent helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032042">
        <property type="term" value="P:mitochondrial DNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905082">
        <property type="term" value="P:regulation of mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18032">
        <property type="entry name" value="DEXHc_RE_I_III_res"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18799">
        <property type="entry name" value="SF2_C_EcoAI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050742">
        <property type="entry name" value="Helicase_Restrict-Modif_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47396:SF1">
        <property type="entry name" value="ATP-DEPENDENT HELICASE IRC3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47396">
        <property type="entry name" value="TYPE I RESTRICTION ENZYME ECOKI R PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="2">
        <location>
            <begin position="68"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="3">
        <location>
            <begin position="284"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="435" mass="48789" checksum="C63E13A2BC4454E0" modified="2017-01-18" version="1" fragment="single">MALRLITGCIPGAIQRHLFQPAYNKVLVRTFKTSSRLTKQFHIEEPATPRPKFQLREYQQRCIDQCVDALENDRRRIGVSLPTGGGKTVVFSNLIDQVKPKNGHGDKVMILAHRKELIFQAAATCKSVHPDKIVDVEMANYHATEDADIVIASVQTLMRGRLEKFNPLDFKMIIVDEAHHAAADSYVSTFNYFGITEPDSDVALVGFSATLQRDDDKHLADVIDEVVFHMDTEQMVTDKHLCDAKFTTVKLVNADLSGVTTRGGEFVIPKLAAAVNNPENNELVLRTYFHFVNTQNVRSALLFGVDIKHVRSLAALFKERGVDADFVTSETKAPVRKERIDKFKKGELSVLMNCGIFTEGTDIPNIDCVLLVRPTRSKNLLIQMIGRGLRLHHLKEFCHIVDFVGVNNANVISVPCLRGLPNDDQINDLTLEEME</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P873</accession>
    <name>A0A1E3P873_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNA polymerase II degradation factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_83556</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040621.1">
        <property type="nucleotide sequence ID" value="XM_019185561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P873"/>
    <dbReference type="STRING" id="683960.A0A1E3P873"/>
    <dbReference type="GeneID" id="30202807"/>
    <dbReference type="OrthoDB" id="5396806at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="494"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="637"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="178"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="227"/>
        </location>
    </feature>
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        <location>
            <begin position="258"/>
            <end position="271"/>
        </location>
    </feature>
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        <location>
            <begin position="306"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="381"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="504"/>
            <end position="527"/>
        </location>
    </feature>
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        <location>
            <begin position="550"/>
            <end position="581"/>
        </location>
    </feature>
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        <location>
            <begin position="637"/>
            <end position="662"/>
        </location>
    </feature>
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            <begin position="663"/>
            <end position="681"/>
        </location>
    </feature>
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        <location>
            <begin position="682"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="733" mass="80742" checksum="2FAB6D3B086EA238" modified="2017-01-18" version="1">MSESTTQKSQQSRRPRGGFRNQSKRFNNNPSSSSSSSKYSDEIKSKLSPLNELFPDWSNEDLVDLINEFDVLEVIIEKITTGAVSKWDEVKKPTKKEKQVVSDEPKHIINKENGSHPKKEFKQRSATTPKEKGLKKTSAPKSTKPTSAPANASSGKPSSSSWAAALANSDPKAKASKQQEQPQESTQESQQSEEQEPQQQQPEKVEEEVKQEQPKEEQKETPKEVKKPAQTPATSNKSWAAIAAPPAPKKKQTPKPKPQAQPQAAPAVVEQPEQKDVEKVVEVTKGVETVSVEDKQESKPEVAPTAVPAVEEQEQQAAPASIPKQQEVSAEIPVTEQPQQKTQRRLQQDAPVVLPSSANQVGNVGGISFGSSLLQEDEKPAQQQQQQQQSAQPQQQQQRQYNNLYNNQQQQQQLPRQYQQAQQQQNNLYQQQYQQQQPQAQAQQQQQPQSQQAGQQQQPPKGAYDQYYAGYQSHLPGYNYGGLDYQYDRNSLYNQQRSAGGSGSYDQSSAASAQQQASAAQQAQTSQFGHQQSPYGIPPQIPGTGIPMNELSTASPVTSSVQPNTAQPTTTGQASAGQQPNVANIPYQTPAFFQNMPYMYYPQQYAYFQQQAAAAQQQQYPPYNQYTAAQQQQQQQQQQQQQQQPKAYGYGVPQGQQQAGQATASNPASQGGQSGTSAPGSQQQGDVDGQQAQGQGQPQQAVQGQAPYYQGVQPSYYGAYANADFSANGRGWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8A1</accession>
    <name>A0A1E3P8A1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_28327</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019181473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8A1"/>
    <dbReference type="GeneID" id="30198719"/>
    <dbReference type="OrthoDB" id="196103at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010291">
        <property type="entry name" value="Ion_channel_UNC-93"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051617">
        <property type="entry name" value="UNC-93-like_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294">
        <property type="entry name" value="ET TRANSLATION PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294:SF59">
        <property type="entry name" value="UNC93-LIKE PROTEIN C922.05C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05978">
        <property type="entry name" value="UNC-93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="449" mass="49604" checksum="E44943ADFBBBC193" modified="2017-01-18" version="1">MLWCSAIVGCTAGLYCATNGLGAGGGKPGYAQVANIDTAIGDVLWLFSSLFAGTLINKLGPNVCMVLGSLAYPMYISGFWFYDKLGIGYYSYLVSGVAAGILFGFLWTTQNYLVTCYATENHKGFFVACNWGVINLGAALGSVIAIGVSKDKTERSAGVPWYLYLIFIIINLGGAVMAIFLKHPKNVVRNDGSKIAVFQNRTLKENLVALKQSLMERRIQLMILPLFSAETILAPAANLNGFMFNVRSRTLNNFSFWAVQIPGAFALYFLLDQKGKSRKMRGYMGLGLTYFFVNCFFIGFYIMYGAWGKAKFDPASPESELPDLDWSMKTYAGPYVWYVCGGIAFGLFLDMRLWIIGAFSNDPQKLSLYSGVIPACQAGGIALAFGLNATSLSFTSIMAIWYVFYMASLPCLILCIKWYITDTNYFAEQDVIVPQEAILDQKEVRFRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8E5</accession>
    <name>A0A1E3P8E5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent DNA helicase CHL1</fullName>
            <ecNumber evidence="18">5.6.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">ATP-dependent DNA helicase chl1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Chromosome loss protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19 20">DNA 5'-3' helicase CHL1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">WICANDRAFT_40995</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">ATP-dependent DNA helicase important for chromosome transmission and normal cell cycle progression in G(2)/M. May have a role in changing DNA topology to allow the loading of proteins involved in maintaining sister chromatid cohesion in the vicinity of the centromeres. Has a specific role in chromosome segregation during meiosis II.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family. DEAH subfamily. DDX11/CHL1 sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="18"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040429.1">
        <property type="nucleotide sequence ID" value="XM_019182560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8E5"/>
    <dbReference type="STRING" id="683960.A0A1E3P8E5"/>
    <dbReference type="GeneID" id="30199806"/>
    <dbReference type="OrthoDB" id="267079at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045005">
        <property type="term" value="P:DNA-templated DNA replication maintenance of fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034085">
        <property type="term" value="P:establishment of sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031571">
        <property type="term" value="P:mitotic G1 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18788">
        <property type="entry name" value="SF2_C_XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001372">
        <property type="entry name" value="ATP-dependent DNA helicase chl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013020">
        <property type="entry name" value="Rad3/Chl1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00604">
        <property type="entry name" value="rad3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF41">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE DDX11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="23" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="24">
        <location>
            <begin position="9"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00045008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00045702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="ODQ61222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="810" mass="92705" checksum="F0675736F2003041" modified="2017-01-18" version="1">MENEDTEANKKKFYHPYDPYDIQIDFMKALNETLTGGYKIGIFESPTGTGKTLSLICSTMSWLRSRRTESEEANDEDDDDEPAWIKESYSNLLSKKNNDAAQAYEAHLTEIANNSKIRQVQVLKESNKKCKRFKKIDAEVQEEDSFLPNDYNSDGDDELNSQRAENLKEKNDALNSEIQKLLKNIDRKDKDEAEGLSKDVKIFFSSRTHSQLNQFSSQLRLPNFPSLVDHQEEHLKYLPLGSRKQLCIHEKVSQLKDVSLLNEACLDMQKQDSKKKCDFYFTQKDMEKQDRAIEFRDTLFSKIHDIEDLNTIGKDFGVCPYYSVRKGIPIAEVVTLPYQLLLQKSSREALKISIKDCVVVIDEAHNLLDTIISLHSVSVSIDDFTVCKQALKKYLSKFSRKLNGGNRINIMKLVKIIDIFLKYVESCGKKSPGKEINLLDVFQGTTGDLLNIKKLEKYLTVSKIAFKIESYMEKENQSSTPTLFKVVEFLKAVSNPSREGKFFFDLKNHVTSLNYMLLDPSEVFKEIVEDARCVVLAGGTMEPIDDYTNYLFPYIDESKIKKFTCGHVIPKENLDVFVVGGKTREFDFSFDKRNDSVMIKDLGSAILSLIKTIPAGVVIFFPSYKFLSDIVNVWKSCSVWDDLNNVKQVFSESSSNNILVDYSLAISQGKGAVLLSVVGGRLSEGINFSDDLARAVMMVGLPFPNVFSGELISRRKYIEEQVWSKTGNRNLAQEATKVFYENICMRAVNQSVGRSIRHKGDYSTIYFFDKRYHTSRIKLKLSNWIKERINDSVSFDQVVLKTVDFFQNRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8I5</accession>
    <name>A0A1E3P8I5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_65985</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040827.1">
        <property type="nucleotide sequence ID" value="XM_019184025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8I5"/>
    <dbReference type="GeneID" id="30201271"/>
    <dbReference type="OrthoDB" id="4046837at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="531" mass="60822" checksum="725C97D4ED1EF4F6" modified="2017-01-18" version="1">MLKQRQLLRQLPHIRSLNFSQRHYSTEISVREKLLKLKESKDIPPSVYSNLLNHNDIITGKEVEIVDEIIDLAKNDSNVTNESRVQLHNFVLKNLVKYDYSLSVVHQSELSDLKGVVDLDSMVEIIKYNPGRVKSSWELFETTMSTIEPDSSEEYQELLSTVLSKLVHGDASEQEDGYIMNESALLRSVFLLKCLKAPSQDLLEKVLARALETDNYQLIQLIGLQSFEQIKNLEPNTNQFVQLWSIWNPSPSSLSKEEIENNLDLWKKLLWRTSEKKESTSPKDNSSHQESFSYLKQLQEQFPKLQVDLTVPKKTDQDSTFQSLANILEESGIFKNPEPKYTPLRVTLLKSYGMSKDGIKTAFQLYHDFISLDKQNVDLYMTTMATSSFYLAVKNNDSRHLMIGEALIPQPIPYKALQAQILGHSSFDIQKSLNLFNDHIKDVSKKVNELGISPATSLIESLITSYLSKKDLQFAHLIHDGAIMNNIITTETAKNRMKAIFKRYGEINGEEDNDKMAVMLKDQVLESLREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9D7</accession>
    <name>A0A1E3P9D7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">mRNA 3'-end-processing protein RNA14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_52687</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="4">Nucleus and/or cytoplasm.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040704.1">
        <property type="nucleotide sequence ID" value="XM_019182697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9D7"/>
    <dbReference type="STRING" id="683960.A0A1E3P9D7"/>
    <dbReference type="GeneID" id="30199943"/>
    <dbReference type="OrthoDB" id="26282at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005848">
        <property type="term" value="C:mRNA cleavage stimulating factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180010">
        <property type="term" value="P:co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072423">
        <property type="term" value="P:response to DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
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        <property type="entry name" value="Rna14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008847">
        <property type="entry name" value="Suf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19980:SF0">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19980">
        <property type="entry name" value="RNA CLEAVAGE STIMULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05843">
        <property type="entry name" value="Suf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="2 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Suppressor of forked" evidence="6">
        <location>
            <begin position="43"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00026188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="659" mass="77797" checksum="107DC29BDBC98C6E" modified="2017-01-18" version="1">MSTVGTPEIEPTPEIGSTSIPSATTSINNKSLKNKKTRLASDVIGQLEDKLKENDRDISVWLELISKIELKDKTQQVRETYDKFFKKFPFFAKQWINYINYELNRGEFDKVENIFKRCLYNTSSVLLWKTYVNYIRRKNNLITGGEEARRVIFQAYEVAINKVGFDPESGSLWDDYFSFIEDWKPVSTWDGQQKMDMKRKLFKKALNIPLNNLENLWGIYTSFETELSPNTARKFIGELSGQYMNSRSWFKEWSNLTKGLIRSDDFVPKQDEYDSFQLSKWLDWVNWEKSNKLELDNDKLIERIEFVYKQAIQHLTFYPELWFEFANFYKENQQHYSSINFQELLNEAILVNPLSFALNFKLIESYEVENNTVLVGKKFSSLIEHLTQIHDGKSLEIEELKNELFAKLSKTISLTYCQYIKSMKRLSGIKEARQIFGLARASKKVLTHHVFVEVANLEHFATNTKTAIKVFDLGLKPAFFSNDGEYIYKYLKFLIKINDDTNSRSLFETTVNVKGKFIESQWLKRIFKIYMSYELNFGQISSVDKLEKKYFEYFPEDSKILVFADRYTFADIQDCDDVSDDLIRYNDLKYSELVPKKSVSSKRRLEDDEQVENENEDYKIKQYVTDEVYNLLRVLPNSSNFQQPVFNNTKLIQLLNRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9H1</accession>
    <name>A0A1E3P9H1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Membrane protein PTM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_12717</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LU7TM family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041266.1">
        <property type="nucleotide sequence ID" value="XM_019180540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9H1"/>
    <dbReference type="GeneID" id="30197786"/>
    <dbReference type="OrthoDB" id="19932at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053937">
        <property type="entry name" value="GOST_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009637">
        <property type="entry name" value="GPR107/GPR108-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053938">
        <property type="entry name" value="PTM1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229:SF1">
        <property type="entry name" value="GH17801P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229">
        <property type="entry name" value="LUNG SEVEN TRANSMEMBRANE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06814">
        <property type="entry name" value="GOST_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21902">
        <property type="entry name" value="PTM1-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133746" description="Membrane protein PTM1" evidence="8">
        <location>
            <begin position="20"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="252"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="363"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="407"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="PTM1-like N-terminal" evidence="10">
        <location>
            <begin position="29"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="GOST seven transmembrane" evidence="9">
        <location>
            <begin position="177"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="465" mass="53192" checksum="DD0F451A31373F03" modified="2017-01-18" version="1" fragment="single">MKLSSIILPLISLSSLAIANRVTLDESYSQSCTGMYDKQAWGGSKNPFISFNLQKYQSSSSESDPKLSVIIFEYQDFDLIGHLLENGHHKYICDDDSISKNYCNESSKGEFIIDTNNNISEILTFETNQLGLQQLVYPIVKTGYYCVASFPLVNDAKFKAQVNFRNSFGEIAASEFPKLTLYGALAIMYAVSMAYYGFNFWKHKHELLPLQKNFLFFFLFLTLETVFVWGYYDLENRKGENDAGVKVYMTFTSILSGFKVSFSFYLLLVISLGYGVVYPKLDKKLLLRCRILTGVHFFFVVFFVVSNYLTSPESQSLLVGIPAIPVAISTAIFYAMVLKSLSTTTALLHSQRQMIKLKMYKNLFRIIFFSLLVLVFGIIVSSFIFIGMSTTELIEQHWKSRFFFLDFWPSLVYFVIFNLIAFIWRPTDTSYMLAASQQLPTDPENAADFELDDIQSLQNDDDDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9K6</accession>
    <name>A0A1E3P9K6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_76160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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            <begin position="1"/>
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    </feature>
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            <begin position="50"/>
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            <begin position="209"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="427" mass="48523" checksum="00218852A04D772F" modified="2017-01-18" version="1">MAKYHSDTESESYRYDDSNNSSDESVTDLDIPDFNDKNAIQIAEYLDSKLHNVNKRSSGVSTSTDSGTKHCQSPPPERLWTPLSQSTPADIPPNKFQPKDILKAKSKLSKLSANANETIQETDDEHEDNDYTNQSTRGPSFQDETPSKAQKKSPAPVNLVEKQNKADETELQELRELKKYLQNLQNGSVILESFKQSNKNIKILTKEQFEKGEQDLKSLEADITMVKKQNSELKEEISILKDSNLSLKEVISLKSTTIEELNTLSEESKSKFKEVQKIKEEKEEIIQTLEKKLSDLIGDTSEEITPERFPSLSFKGSNGKSVYDTLELGLVDSLNLSKSHNIIKNILINLLVPFSETKETIPKIAKLLESEDVLCEFSNKLHKLIYEQDINISKFQNEQNGNDHLRQCTSQMYKNVETLFKELKPNV</sequence>
</entry>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
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    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ62101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="23301" checksum="7EE1EB90AA834854" modified="2017-01-18" version="1">MAIKYKDLLPVANGLIIAGVFFFLGLLMGNFPYDCKTLFTQEVTQEAFDNSLRHYQTWAEIPRIPLHILHFFIGLGFIDTKYFEYGSLLLYVLAVCVYLTNLKTGAESALYGDWGEVDSNTGINVIAASSAMIIFLLGGIITLQAGLFYGEWDYQQRLEVFNREEAERAAAEASGPVKTSTPKNGKSGSKAENNAAKASGSSVKSSVSKKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9U7</accession>
    <name>A0A1E3P9U7_WICAA</name>
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        <recommendedName>
            <fullName evidence="1">DRBM domain-containing protein</fullName>
        </recommendedName>
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    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
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            <taxon>Eukaryota</taxon>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="527" mass="59874" checksum="F33DB434C0170880" modified="2017-01-18" version="1">MTDLKHMNDDQISSFIIDVSRRRIRDAEMESTADDIASIAFNAGQVSQSIKRIIANPSKQQIEQFKEATTDKRVLYLLESHDVSLGLELKNQYDLGKLDIFDEILNYQHNFVEVEDDLIMDNFIKNSEYFLPPIPDIEDQELKEDIEGNRGLVNYSDLGRSYYENFVNEYLMHRFGDRDIKKLAVYANNHKVASRWARLYELPLDLKVIPYETFFAYVGALISQFGFNSNDLEFEIKNWLTILLEPILMQFDDKIQLKINAKDEIRSLLGHGNIEFRHVFTSTHFEPIHLVQIYANENMLLATSSSDSLANSEARASTIALSNTKLIDRAQKSIKKANESRNKASLSPHVPDPNIQHRYPPQPTYPTYGSYHPAPAPMAGPNMAGPNLAMGHSPYMDQSKAVVQAGHGAPAAAYPQGLVNQDYQRQLQQASDSVFECPLVTDEAAIDTSSKEKLNQLLIKSRINPAEYKTDKLNNSDVQVTCYLDNVPIAKALSTNKKKAGQICAQFILNHWNFFHDRFNKFKPGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA48</accession>
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        <recommendedName>
            <fullName evidence="7">FAD dependent oxidoreductase domain-containing protein</fullName>
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    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_86782</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DAMOX/DASOX family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019186071.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3PA48"/>
    <dbReference type="GeneID" id="30203317"/>
    <dbReference type="OrthoDB" id="2015447at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003884">
        <property type="term" value="F:D-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023209">
        <property type="entry name" value="DAO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530">
        <property type="entry name" value="D-AMINO ACID OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530:SF26">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE SUPERFAMILY (AFU_ORTHOLOGUE AFUA_5G13940)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000189">
        <property type="entry name" value="D-aa_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="7">
        <location>
            <begin position="4"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="44"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000189-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="346" mass="38991" checksum="1ED5C9DCBC986DCC" modified="2017-01-18" version="1">MSEIVVVGAGVIGLSAAQALCEKGYKVKVVAAHFPTDPLNSRYTSPWAGAHYRPFPSKSPKEFEESQLTRKTLEYFEKLAVEEPQSSVKFIDGIDYLEEKGLYDVKAKGYYEDIQYLKEIPKNQLPKGVEFGAKYRTWVLNSPLYIQYLQRKLTFKYGVEFIRTELTSLKQVSQSFPNTIIVNASGLGLQYNGGYDPKSYVIRGQTLLVRAPEGHPYEKQTITHQSKDGLWTFVIPRPLDGGLIVGGTKQVNDFQNTPKDEDTKQLIERSRALYPEIFIDGELDIKNINVGFRPAREGGIRIEIEKVGKQYIVHDYGFGGMGYELSRGSAEEVLKLVESIPTVSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA78</accession>
    <name>A0A1E3PA78_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial group I intron splicing factor CCM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_66133</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040994.1">
        <property type="nucleotide sequence ID" value="XM_019184030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA78"/>
    <dbReference type="STRING" id="683960.A0A1E3PA78"/>
    <dbReference type="GeneID" id="30201276"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051222">
        <property type="entry name" value="PPR/CCM1_RNA-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47942">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="237"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="599" mass="68582" checksum="7B6865B8930F1A92" modified="2017-01-18" version="1">MITSFKSLSRAGTSNIRLFSTSSCSRGKIRKFLKLAARDDIYIKPTSNFEHHQKKARKVKAGEERTAADLRKQKAATILQKVYNEDVDHKKLGPTSNEDLKYFQHADRKTLYTLLGVTEDQILDSVVVTKSVRAFLKRDQTEKAMTLAKLAQSKGIVSMNFIVAYFVQQGKINAALDLHTMRKKWNVSLNEQSMTMLFDEISAYRPVDDQQPLTDAQAKRVLEIYLASTRKSDYKPNLIHFNTCISALFRANDQSHAMNLFKQMEDINIRPDVKTYTIVLQGLAHAKNDIWAVVNAIDIFPKLLTFPEHKLDNHVLEALVRVFTNREKLYLIKRGIQIAQSCFQIPDGVPKIDNFKRLAPSQKGEKLQIPDVFTIENKIEPTASLIDTIMLAASKIGDDKLAIKFFETFKEEGYTSSIDRALVHRYLTVSQLEDKKNAGENSIKIYKTFTQSDLFRHIKLNDQTKYIVFNGLARQVNSTYEEKKQSLRNNKKANDALGFVEEFMEMVEKPYSLRVFSGYLKAVHKLSSSGKRTSMVKERLQVMRKYVSGKSFKDLNCTKPELDAFIKHLNEVEKRFPREPKTDKMITITGNDLTATQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAB2</accession>
    <name>A0A1E3PAB2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_25656</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041566.1">
        <property type="nucleotide sequence ID" value="XM_019181170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAB2"/>
    <dbReference type="STRING" id="683960.A0A1E3PAB2"/>
    <dbReference type="GeneID" id="30198416"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015193">
        <property type="term" value="F:L-proline transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015812">
        <property type="term" value="P:gamma-aminobutyric acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015824">
        <property type="term" value="P:proline transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000006">
        <property type="entry name" value="General amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF36">
        <property type="entry name" value="PROLINE-SPECIFIC PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="56"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="343"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="388"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="417"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="459"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="487"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="10">
        <location>
            <begin position="56"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="558" mass="61285" checksum="8542C1B814595076" modified="2017-01-18" version="1">MSTHLRHTSSNKDEKQVYDIPNVETGESSTGFVDDLESQNSHDLADDLHQGLKARHIQLIALGGCIGTGLFVGSGSTLATCGPAGLLTAYLIMSTVIYFIMNELGEMVCYLPTKGGAISDIAVRYVDESLGFATGWIYYYTFVILLCTEITAAAIVIEYWTDKVHIAVWITIFLVVIIGLNFLAVKYYGEAEFWFASLKIFCIIGLIIAGIVIFFGGGPNQHKVRGFQYWNNPGAFVEHLSHGNTGKFLDVWTGVIKSGFAFIVGPELVAIAAAETENPRRNIAKAAKRFVYRLMFFYVAGSLVIGIMVPSNNPNLLNGSSSAAASPFVIGIRSVGIKGLNHVINAAILTSAWSSGNSFFYAASRTLLSLAKEGKAPKVFLKINRNGVPYVACALTALIGVLSYLNVSSGTSKVFNWFSNISTISGFIGWIVIGIAYLRWRKAVIYNGLWDRVPFKTILQPYGTYYSVGFITLICLTNGYATFFGKFAAADFVAAYITLPVFFLLYFGHKIFTKNWKWAYPVSEIDVLSGLEHVEKLTETAPTPNPTTWYGKVWAWAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAF9</accession>
    <name>A0A1E3PAF9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_24853</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041565.1">
        <property type="nucleotide sequence ID" value="XM_019181065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAF9"/>
    <dbReference type="STRING" id="683960.A0A1E3PAF9"/>
    <dbReference type="GeneID" id="30198311"/>
    <dbReference type="OrthoDB" id="5419460at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032185">
        <property type="term" value="P:septin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36414">
        <property type="entry name" value="PROTEIN SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36414:SF1">
        <property type="entry name" value="PROTEIN SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="92"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="266" mass="29649" checksum="CC90D93CF7BFE8F9" modified="2017-01-18" version="1" fragment="single">LLLILIVLSGSIDHFPINKFYWIEADTSGMGNAPSLSRWTFWGLASEQNGKNSKETLSPAYPFSPVDNFDTTENVPSDFVSNRDTYYYLTRFAFAFFWIALAFVGVSLLISIFSVFSYSIIKVNSWLTTLALIFDAGAVAFMTAAIVLGRSAFKSENRYVHLGPALLGIAWASVAVLIILFFVSWGELISASYKKHKSRVRAEKGEEAYIPQNQQNEPIPAPPAAGQTDYTAEDPYAQQKQGTLNNESGIKFFKIRRHNKEDGESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAH4</accession>
    <name>A0A1E3PAH4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_60428</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041575.1">
        <property type="nucleotide sequence ID" value="XM_019182992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAH4"/>
    <dbReference type="GeneID" id="30200238"/>
    <dbReference type="OrthoDB" id="10589159at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031511">
        <property type="term" value="C:Mis6-Sim4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025207">
        <property type="entry name" value="Sim4_Fta4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13093">
        <property type="entry name" value="FTA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="196"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="230" mass="27167" checksum="4D81BD41CA8E5D20" modified="2017-01-18" version="1">MSNSYQSLNKQIKTHVKKLETPFKLDDTLLEICEESLSNNNNESRPLKPRELSKILKDANIILGNGYHRYYSTQAISQLTEQIFNLREQQQLDQQDLIDFLENLELPINLESDFKVYSIKDIIDQIENIPEIPQQQDEENEQFTQYNTKRQEILNKISQLENLKKKTGLVKNYKNLINLKIGERSNIQKNIQSSKNTELSNEITKLRINLERYKTLNDAQKEKFKQLLHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAJ4</accession>
    <name>A0A1E3PAJ4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_60447</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DCP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041595.1">
        <property type="nucleotide sequence ID" value="XM_019182995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAJ4"/>
    <dbReference type="STRING" id="683960.A0A1E3PAJ4"/>
    <dbReference type="GeneID" id="30200241"/>
    <dbReference type="OrthoDB" id="440673at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098562">
        <property type="term" value="C:cytoplasmic side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098745">
        <property type="term" value="C:RNA decapping complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010334">
        <property type="entry name" value="Dcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290:SF0">
        <property type="entry name" value="DECAPPING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290">
        <property type="entry name" value="TRANSCRIPTION FACTOR SMIF DECAPPING ENZYME DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06058">
        <property type="entry name" value="DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="19253" checksum="72FE333524B0557B" modified="2017-01-18" version="1">MSEIPSTTPTPPPATETILDNEFSLYTKTLTFNVIGRYDPKIDRLLYHTSHCQMYKFGETQEWEKIDYSGVLAIYSRKQNEDEVDLDDIYDHGIIILNRTSPENFSVGLLSKNQSKNLGISEVKVEYQSEYIMLKNVEGDIFGFWVHEEKDRESVYSLIKAIVNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAM1</accession>
    <name>A0A1E3PAM1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_76609</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041648.1">
        <property type="nucleotide sequence ID" value="XM_019184419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAM1"/>
    <dbReference type="STRING" id="683960.A0A1E3PAM1"/>
    <dbReference type="GeneID" id="30201665"/>
    <dbReference type="OrthoDB" id="4020988at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990811">
        <property type="term" value="C:MWP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990810">
        <property type="term" value="P:microtubule anchoring at mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052778">
        <property type="entry name" value="Centrosome-WD_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16220">
        <property type="entry name" value="WD REPEAT PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16220:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN WRAP73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="527" mass="59834" checksum="2E4717BD5EF1B184" modified="2017-01-18" version="1">MSLVDESARFTYSGHKPVPSPCGKYIAYLNGSKLVIRKSDLLTLDRLIKIKTKNSNGFLRISQITWEPLYNGQSNKVALLIDNENTVKIYDMNDEKVDISIIEDDVFGIEKFQWLPKGEESIETAYDGSKQIVVFTKNELYAKIYSLDYSQVLISIEKPKFNKVLIKPNSNIFSIITSKINGYVVRNYLNNGSSVAHFNNIDIDSVLNLTDSINWSPNGQWLLCNDSVIGETKVSVYSLFNEVLSQSEAIFRYEDVSDPLGAIDVKWGEGNEHVLISDHHEQIHCLDVYTGLNIQYTLQHKPQTETSKIWKQDNINWKYVRKQTSYKFPQIGNLPLHLSGVTKFLVNGNYTFVTVKSMPGVVFIWDVNGEDSNEPKEVLVTNTRIKDMIVANENKDLLLVVNEDSITLWHTEWSSPIVIPWEDDEGSSLKGACFINATRGSARIMLWTSNLFEVVQIKLSSTSEFEGMDSEQIDVIRNGSPALIDDLTHVVELANGVQQSEWGHNNTNTIQLDDTFMSKKQHKSIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAP6</accession>
    <name>A0A1E3PAP6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Tethering factor for nuclear proteasome STS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_60496</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in ubiquitin-mediated protein degradation. Regulatory factor in the ubiquitin/proteasome pathway that controls the turnover of proteasome substrates. Targets proteasomes to the nucleus and facilitates the degradation of nuclear proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Binds the proteasome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the cut8/STS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041645.1">
        <property type="nucleotide sequence ID" value="XM_019183004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAP6"/>
    <dbReference type="STRING" id="683960.A0A1E3PAP6"/>
    <dbReference type="GeneID" id="30200250"/>
    <dbReference type="OrthoDB" id="10061064at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071630">
        <property type="term" value="P:nuclear protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031144">
        <property type="term" value="P:proteasome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1590">
        <property type="entry name" value="Tethering factor for nuclear proteasome Cut8/Sts1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013868">
        <property type="entry name" value="Cut8/Sts1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038422">
        <property type="entry name" value="Cut8/Sts1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28032">
        <property type="entry name" value="FI02826P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28032:SF1">
        <property type="entry name" value="FI02826P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08559">
        <property type="entry name" value="Cut8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU368013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="337" mass="38986" checksum="BA3A96CEB27777F2" modified="2017-01-18" version="1">MSSVLPSSFSWGFDPKIEDNSQQNEQSSKDSSRQHRINQEILANLQSSMGTPMKSKRKHQDEEQEVTPRKNVHNLRKVDKFRVSKKEVSHKRSRVQRIIGQPLPTNRIIEVLDKKNLQHLLTNLINLHPEITHDVYRCAPKPTVSGSIEVLSNKIDVIINNLPYKVDASSEYSYMRVKPLIEDFLNALSDYTLHFLPPVETQPSNSLDFLDQATDLLHKLPNFTKLSNNYFKELAYEQLSHTWCICLKEFIKNPSTTNNAGLLLLINNNWEMKLKKHNEESNNKLESVVEFFKDEISWLDDELNEKDGPSKLANLNNFSPHNSPLHGFKTNLLDNFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAW9</accession>
    <name>A0A1E3PAW9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_17209</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041760.1">
        <property type="nucleotide sequence ID" value="XM_019180732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAW9"/>
    <dbReference type="STRING" id="683960.A0A1E3PAW9"/>
    <dbReference type="GeneID" id="30197978"/>
    <dbReference type="OrthoDB" id="5778525at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057072">
        <property type="entry name" value="bHLH_INO4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052207">
        <property type="entry name" value="Max-like/E-box_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15741">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX ZIP TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15741:SF27">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23181">
        <property type="entry name" value="bHLH_INO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="12"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="86" mass="10067" checksum="F7907EB40CE5CBD7" modified="2017-01-18" version="1" fragment="single">KTKKTVFLTEEEKKKHHIESEHKRRQAIRDAFSRLVELVPELKPSDNRSEILILNKSADYLDALLEEQKSLVGQLEKKGVEVEERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBC0</accession>
    <name>A0A1E3PBC0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_60761</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041912.1">
        <property type="nucleotide sequence ID" value="XM_019183057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBC0"/>
    <dbReference type="GeneID" id="30200303"/>
    <dbReference type="OrthoDB" id="4073891at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="412"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="456"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="504"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="533" mass="60157" checksum="3527CD576610229B" modified="2017-01-18" version="1">MSDDQDPEEADASILRESENAPLPSHPEFPLIEASVFPQSSKDNEKEVDTKNEPDTQLSEIYGESRFQAKYRQLLNNKQDFMSFFGKSAKPKSPVSSYNSPEPLLPHHATSNPLILQDPEHLLFDQEIANLTEDGSLKDNSSTINGNVAPNTTERDLTGLKLLEARSNSIDEVISQLYQKENDSTDEFNHVRDAGILDKPHTEKESFSMLLKDKRGPMKYLMAIFLLILSLVCALIFSSFPLFSCQHKNGACFPQLTVQITDKSPAAKTALVTVKEALKVLTYLAMDFGDSSSELDLINSRLDTYYQEKVIDTFSVNTLYQLNFLGYCRISSRKGRNFCMKSFGLDLLSVFVRDAGVQLGELTRTNVDIMGDSFAIAYELAITGFNQFTNKENHSVEYIDYAILLQKFSKGLGFLTVSQFVIGCVLLAFAILLLVLKYKFVLKRLSMSQSYIVKKWFLISMVSLSFLNLFTGFLICSLTFEFILRLSEVGQNVGIANVNRASGFSFIWITFVFEVFTCCFVIYLANGFRKRMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBI5</accession>
    <name>A0A1E3PBI5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_88522</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041948.1">
        <property type="nucleotide sequence ID" value="XM_019186159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBI5"/>
    <dbReference type="STRING" id="683960.A0A1E3PBI5"/>
    <dbReference type="GeneID" id="30203405"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="119" mass="13103" checksum="13FE9EBB38F8BDD5" modified="2017-01-18" version="1">MVNTKVALQHLKRLGYKADSAIHGVEALAKCAKKIKDTGRNYDLILMDIQMPLKDGIETSKDLEELYGGTDLLPIIVALTANVEMEDRDRCISCGMSDFISKPILPEKLAKVLRTAGQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBJ6</accession>
    <name>A0A1E3PBJ6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Coatomer subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Beta-coat protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_50568</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
            <orientation evidence="1 10">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasmic vesicle</location>
            <location evidence="10">COPI-coated vesicle membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041996.1">
        <property type="nucleotide sequence ID" value="XM_019182655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBJ6"/>
    <dbReference type="STRING" id="683960.A0A1E3PBJ6"/>
    <dbReference type="GeneID" id="30199901"/>
    <dbReference type="OrthoDB" id="10261439at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011710">
        <property type="entry name" value="Coatomer_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016460">
        <property type="entry name" value="COPB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029446">
        <property type="entry name" value="COPB1_appendage_platform_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10635">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10635:SF0">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07718">
        <property type="entry name" value="Coatamer_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14806">
        <property type="entry name" value="Coatomer_b_Cpla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005727">
        <property type="entry name" value="Coatomer_beta_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5 10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="12">
        <location>
            <begin position="17"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer beta subunit C-terminal" evidence="13">
        <location>
            <begin position="661"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer beta subunit appendage platform" evidence="14">
        <location>
            <begin position="798"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="486"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF14806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ62789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="940" mass="105609" checksum="D4503815625739DF" modified="2017-01-18" version="1">MSEAAYTLVFDPNPASKVSVQEYRTLLEKGKDESKIDAMKQILIAMLDGNPMPELLMHVIRYVMPSRNKELKKLLYFYWEIVSKLDSDGKLRQEMILVCNAIQRDLQHPNEYIRGNTLRFLTKLKEPDLLEQLVPSARACLEHRHAYVRKYAIFAIYSIYLVSDHLIPDAPEIINDFLITETDPTCKRNAFIALGNLDREAALRFIQENVAALDTIDPLLQLAFIEFIRKDNAPELRTRYINLLSELLESTSNTVIYEAANTLTVLTSNPSFISLAANKFIELAIKESDNNIKLIVLERIHDIHKKNAGILEDLTLDILRILSTPDIDIRKRAIEIALDLISSRNVDEVLKLLKKELQKTVTSDADKSVEYRQLLISAIHQTAIKFGQIAASVVDLLLEYIADLSSTSASDVLSFIKEVVEKYPDLRSSIVHRLLLSLDNIRSGKVFRGALWIIGEYSLEEKDIQEAWKHIRSSVGEIPIIQSERKRIGEPEGEEKPEEHKANGSKPVVLPDGTYATESALTSEKKSTKEEVRPPLRQFILDGEFYTSAVLASTFVKLILRFQEISQNAQILNALKAEGLLILVSILRVGETSYVSKKIDEDSAERILTAITYIAEGKESKLLEIAFLEATRDAYKVQVAAEDKRKAKLDEAEIAKNSQQVDDVIQFRQFGKSEGTDVIEEDLTLATGVKVEEDLSSRLNKIVQLTGFSDAVYAEAYVKVHQFDVVLDVLLVNQTTETLRNLHVEFATLGDLKVIDKPTTQNIPPHGFFKVQTTVKVTSADTGVIFGNIVYDGGHGFENKVVILNDVHVDIMDYIKPAKCSENKFRTMWNEFEWENKIVIKSNLPSLKDYLDELLKGTNMTCLTPGAVIGEECQFLSANLYSKSSFGEDALANLCIEKKTDGPIVGHVRIRSKGQGLALSLGDRVAAIARKTNKAKLTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBK2</accession>
    <name>A0A1E3PBK2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Regulator of rDNA transcription 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RRT14</name>
        <name evidence="9" type="ORF">WICANDRAFT_76965</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Involved in ribosome biogenesis, probably through modulation of rDNA transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Nucleus</location>
            <location evidence="2 7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the RRT14 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042010.1">
        <property type="nucleotide sequence ID" value="XM_019184478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBK2"/>
    <dbReference type="STRING" id="683960.A0A1E3PBK2"/>
    <dbReference type="GeneID" id="30201724"/>
    <dbReference type="OrthoDB" id="3981070at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031404">
        <property type="entry name" value="Rrt14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17075">
        <property type="entry name" value="RRT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="190" mass="21476" checksum="CEAD7FEFC58973AA" modified="2017-01-18" version="1">MSITFKNPSKSQKTVNKLLSRVLPGHTATSTTPSSSSSNLSSTSQQLTSSHNSNTSHKVLKQQKKKQKQAIKISESQRQKLMAEAQRRVLKEHSTTGKLTPQEALELKKYVKKNVVDITSWHNEDDELDEVQQEILELKQKEPRFRGKRVSNKKSAKKQMDGIYNKKEMIRYPGLTPGLAPVGMDDEDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBP8</accession>
    <name>A0A1E3PBP8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_60871</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042032.1">
        <property type="nucleotide sequence ID" value="XM_019183071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBP8"/>
    <dbReference type="STRING" id="683960.A0A1E3PBP8"/>
    <dbReference type="GeneID" id="30200317"/>
    <dbReference type="OrthoDB" id="21467at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12307">
        <property type="entry name" value="RRM_NIFK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46754">
        <property type="entry name" value="MKI67 FHA DOMAIN-INTERACTING NUCLEOLAR PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="154"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="83"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="127"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="256"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="279" mass="31735" checksum="4F9581408E419245" modified="2017-01-18" version="1">MGTKIIKKSSIKPKKQGSIARPKKESKKVIIKAKKATEPEFDQDEESEEEITHIPSDLSGSESSGSESEVEEDAAENSKSAEAESEDEEDLGGFSSTDDEQDEQDDDEDSEEEEEKEEPKKSKKQEKKKESHEVKKLPKVDSNVSKKSKKAKKGVIYIGRLPDGFEEKELSKYFNQFGEITNVKLARNKKTGKSRHFAFLEFSNVDDAKIAQDTMNNYLLMNHQLKVELVQEDFKSSKKSKKSYFRVSKLKKDVKSLNEKDQAKREKRKEALKAAGINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBZ4</accession>
    <name>A0A1E3PBZ4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Signal peptidase complex catalytic subunit SEC11</fullName>
            <ecNumber evidence="4 15">3.4.21.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_77093</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalytic component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Specifically cleaves N-terminal signal peptides that contain a hydrophobic alpha-helix (h-region) shorter than 18-20 amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the signal peptidase complex (SPC) composed of a catalytic subunit SEC11 and three accessory subunits SPC1, SPC2 and SPC3. The complex induces a local thinning of the ER membrane which is used to measure the length of the signal peptide (SP) h-region of protein substrates. This ensures the selectivity of the complex towards h-regions shorter than 18-20 amino acids. SPC associates with the translocon complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the peptidase S26B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="4 15"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042140.1">
        <property type="nucleotide sequence ID" value="XM_019184499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBZ4"/>
    <dbReference type="STRING" id="683960.A0A1E3PBZ4"/>
    <dbReference type="GeneID" id="30201745"/>
    <dbReference type="OrthoDB" id="10257561at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009003">
        <property type="term" value="F:signal peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019756">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015927">
        <property type="entry name" value="Peptidase_S24_S26A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02228">
        <property type="entry name" value="sigpep_I_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF6">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00717">
        <property type="entry name" value="Peptidase_S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00728">
        <property type="entry name" value="SIGNALPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00501">
        <property type="entry name" value="SPASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Peptidase S24/S26A/S26B/S26C" evidence="16">
        <location>
            <begin position="21"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ62933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="166" mass="18815" checksum="AD4A80DE7BC0E96B" modified="2017-01-18" version="1">MNLRLQLAQVLNLIYVVTSAYMFWKGLSVATNSSSPIVVVLSGSMEPAFQRGDILFLWNRNERSKVGDVVVYEINGKSIPIVHRVLREHHNDKKQLLLTKGDNNPVDDLGLYNYNQNYLDKEKDIVGTVKGYFPKVGYVTILLTENQYFKYGLLGLMGLSSLLSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC31</accession>
    <name>A0A1E3PC31_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_89157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042135.1">
        <property type="nucleotide sequence ID" value="XM_019186176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC31"/>
    <dbReference type="GeneID" id="30203422"/>
    <dbReference type="OrthoDB" id="2253354at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="121" mass="13824" checksum="6037C14B074E6C3A" modified="2017-01-18" version="1">MALVNKLFQRKNPKDYLKSTSDKFGNQFGGLKKNELGLDDQFSEDALNKFLGLDPTPAEASKSQFNEPQPIEKLSHEDIDFQVKNEKELLNYKLDPHKHVVVENPGDYVDAAKDTKLTRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC95</accession>
    <name>A0A1E3PC95_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Lysophospholipase NTE1</fullName>
            <ecNumber evidence="3 14">3.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Intracellular phospholipase B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_21795</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the NTE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="3 14"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041799.1">
        <property type="nucleotide sequence ID" value="XM_019180919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC95"/>
    <dbReference type="STRING" id="683960.A0A1E3PC95"/>
    <dbReference type="GeneID" id="30198165"/>
    <dbReference type="OrthoDB" id="421051at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034638">
        <property type="term" value="P:phosphatidylcholine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071071">
        <property type="term" value="P:regulation of phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000007">
        <property type="entry name" value="Lysophospholipase NTE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000013">
        <property type="entry name" value="Lysophospholipase NTE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001423">
        <property type="entry name" value="LysoPLipase_patatin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056556">
        <property type="entry name" value="NTE1_P-loop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF29">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE SWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24179">
        <property type="entry name" value="NTE_Ploop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01237">
        <property type="entry name" value="UPF0028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 13 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13 14" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="11 13 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="16">
        <location>
            <begin position="441"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="16">
        <location>
            <begin position="549"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="17">
        <location>
            <begin position="920"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="257"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="13">
        <location>
            <begin position="924"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="13">
        <location>
            <begin position="951"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="13">
        <location>
            <begin position="1071"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="282"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="13">
        <location>
            <position position="953"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="1071"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="1224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ62592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1224" mass="137124" checksum="A5DAD9DD4A176564" modified="2017-01-18" version="1" fragment="single">DNRKRVGSYLDEFLTAIKIFGYLEKPVFNELTRSMRTQKLDADEVLYVDESLGFAIVVEGNVQIYTKVNNQTNNDTSFSMDDDQSEGSSEIFVLNEERYQLLNEVKTGNPLSSLVNILSLFTDEDDANGIQSPTIRQRLPDGSFAQLHPDVDIPTLSLDHEDVFPTTPNIIARATSDSTIAIIPADAFRRLKRKHPRSSSHIVQMILTRLYRVTFQTAHNYLGLTREIFNTEIKLNSEAKLELPSYLHDGVKQRFKKGKHSRGGAINERGRPTNKTPKLGNRSESSTSTLMTPSTKLEARPKVKRIESRHVVLDSRDSFNPGDLLSNVPLSRKELSYQAIAEDDVMNRSFSAEEETEDTSLRIALVEGMFKFLGIDKESIIPRRPSASVSAISSPMPQGLGLFPTFPIETYSSRAPKTRTMSTTSSRLSFCESPSEHVDFEEAKTDFANEIEILQIEQGTTIIEQNSRNNGLYYVVSGTLEVTYTDPSSNVEHVLYTVNPGGVAGYLGALVGYKSFVSLRAKTETYVGFLSKKDLERLCEKYFMIYLSIAKSLIKSLSKKILKLDSALEWIQLDAGETLFKQDTDANGIYIVLSGRLRSLSENETTNDVKILAEYGQGESFGEVEVLTAAKRSSTFVAIRDSETARIPRTLFEILALENPAIMIKVSRIVARTIKAESSESFSPNTHVPSVTKFKNPNSNYRTVTILPVNHGLPVREFASKLIQAFKNVNRSVIGLDQASTLNYLGKHAFDRLSRLRQSGFFSDLEERYQIVVYIADTAVNSSWTSTCIQQGDCVLLLADALGDTNIGDYERLLMKTRTTARTELILLHPEKYVEPGLVNRWLKNRVWVHSHHHIQFNLQRTGNAHPERGQNSFASNIRTKVENLRSKYVGPKKYYTSSLPHKNDFMRLARLLSGQAVGLVLGGGGARGFSHLGVLKALQENGIPIDMIGGTSIGSFIGGLYARDYDLVPIYGRAKKFSGRISSVWRTLSDLTYPTTSYTTGHEFNRGIWKVFGDSRIEDFWIQFYCNSTNITNSTMDIHSSGYAWRFIRASMSLAGLLPPITDNGSMLLDGGYVDNLPVGEMKSRGASIIFAVDVGSVDDRTPMKYGDSLSGLWIMFNRWNPFSKHPNVPTMSEIQMRLCYVSSVNALERAKNTPGIIYLRPPIENYATLDFGKFEEIYNVGSIYGHMVLKELAETGKLPQIAGANYRLYNKEMPALLLHRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCB7</accession>
    <name>A0A1E3PCB7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">beta-glucosidase</fullName>
            <ecNumber evidence="4 11">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_25399</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Glycan metabolism; cellulose degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="4 11"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042137.1">
        <property type="nucleotide sequence ID" value="XM_019181139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCB7"/>
    <dbReference type="SMR" id="A0A1E3PCB7"/>
    <dbReference type="STRING" id="683960.A0A1E3PCB7"/>
    <dbReference type="GeneID" id="30198385"/>
    <dbReference type="OrthoDB" id="416222at2759"/>
    <dbReference type="UniPathway" id="UPA00696"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.300:FF:000002">
        <property type="entry name" value="Probable beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050288">
        <property type="entry name" value="Cellulose_deg_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019800">
        <property type="entry name" value="Glyco_hydro_3_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715:SF2">
        <property type="entry name" value="BETA-GLUCOSIDASE F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00775">
        <property type="entry name" value="GLYCOSYL_HYDROL_F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 11 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 11" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133784" description="beta-glucosidase" evidence="12">
        <location>
            <begin position="18"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type III-like" evidence="13">
        <location>
            <begin position="785"/>
            <end position="856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ62930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="868" mass="94198" checksum="C1C9A3CA257D2983" modified="2017-01-18" version="1">MLLPLYGLASFLALSQAALVNNSAPQASDDDPFNHSPSFYPTPQGGRINDGKWQAAFYRARELVDQMSIAEKVNLTTGVGSASGPCSGNTGSVPRLNISSICVQDGPLSVRAADLTDVFPCGMAASSSFNKQLIYDRAVAIGSEFKGKGADAILGPVYGPMGVKAAGGRGWEGHGPDPYLEGVIAYLQTIGIQSQGVVSTAKHLIGNEQEHFRFAKKDKHAGKIDPGMFNTTSSLSSEIDDRAMHEIYLWPFAEAVRGGVSSIMCSYNKLNGSHACQNSYLLNYLLKEELGFQGFVMTDWGALYSGIDAANAGLDMDMPGEAQYFGGNLTTAVLNGTLPQDRLDDMATRILSALIYSGVHNPDGPNYNAQTFLTEGHEYFKQQEGDIVVLNKHVDVRSDINRAVALRSAVEGVVLLKNEHETLPLGREKVKRISILGQAAGDDSKGTSCSLRGCGSGAIGTGYGSGAGTFSYFVTPADGIGARAQQEKISYEFIGDSWNQAAAMDSALYADAAIVVANSVAGEEIGDVDGNYGDLNNLTLWHNAVPLIKNISSINNNTIVIVTSGQQVDLEPFIDNENVTAVIYSSYLGQDFGTALAKVLFGDENPSGKLPFTIAKDVNDYIPVIEKVDVPDPVDKFTESIYVDYRYFDEYNKPVRYEFGYGLSYSNFSLSDIEIETLQPFSENAEPAANYSETYQYKQSNMDPSEYTVPEGFKEFANYTYPYIHDASSIKANSSYDYPEGYSTEQLDGPKSLAAGGLGGNPHLWDVGYVVTAKVTNQGPYAGGFAPQLYVGYPDNDEFPTPPVQLRGFEKVFLDKDYSETVSFELTRKDLSVWDTKTQSWIVLRGDYKIYVGQSSRQLDLVTTLTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUJ1</accession>
    <name>A0A1E3NUJ1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">orotate phosphoribosyltransferase</fullName>
            <ecNumber evidence="5">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_86244</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="5"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036003.1">
        <property type="nucleotide sequence ID" value="XM_019186021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUJ1"/>
    <dbReference type="STRING" id="683960.A0A1E3NUJ1"/>
    <dbReference type="GeneID" id="30203267"/>
    <dbReference type="OrthoDB" id="5553476at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046132">
        <property type="term" value="P:pyrimidine ribonucleoside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000008">
        <property type="entry name" value="Orotate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46683">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46683:SF1">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="9">
        <location>
            <begin position="53"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ56796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="221" mass="24087" checksum="B044D93651204D0D" modified="2017-01-18" version="1">MQDYQKNFLDLAVESKALRFGEFTLKSGRISPYFFNLGLFSTGKLLSNLASSYAIAIISSGLEFDVIFGPAYKGIPLAAITASKLAEIGGSKYEKISYAFNRKEKKDHGEGGSIVGANLEGKRILIIDDVMTAGTAINEAFDIIKAENGNVVGTIIALDRQERTVDSQLSATQVVAKKYDIPVLSIVSLTDIINYLSGRISDEEKSKIEQYRAEFAPANIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUL5</accession>
    <name>A0A1E3NUL5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Initiator tRNA phosphoribosyl transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_36715</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036033.1">
        <property type="nucleotide sequence ID" value="XM_019182461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUL5"/>
    <dbReference type="STRING" id="683960.A0A1E3NUL5"/>
    <dbReference type="GeneID" id="30199707"/>
    <dbReference type="OrthoDB" id="45256at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043399">
        <property type="term" value="F:tRNA adenosine(64)-2'-O-ribosylphosphate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019988">
        <property type="term" value="P:charged-tRNA amino acid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007306">
        <property type="entry name" value="Rit1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033421">
        <property type="entry name" value="Rit1_DUSP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033449">
        <property type="entry name" value="Rit1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31811">
        <property type="entry name" value="TRNA A64-2'-O-RIBOSYLPHOSPHATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31811:SF0">
        <property type="entry name" value="TRNA A64-2'-O-RIBOSYLPHOSPHATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04179">
        <property type="entry name" value="Init_tRNA_PT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17184">
        <property type="entry name" value="Rit1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007747">
        <property type="entry name" value="Ribosyl_Ptfrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rit1 N-terminal" evidence="2">
        <location>
            <begin position="20"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Rit1 DUSP-like" evidence="1">
        <location>
            <begin position="341"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ56826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="50082" checksum="BE9B5113DD676328" modified="2017-01-18" version="1">MDKQDLELLRESFNDISKQVRKSSRSVKNRLHSILLDSQWVAQVQSAYNRPLIPNERCGLWYVPPELIKETAYFKSTDGHTSQWSFSTRRLNFHLFDIMVENQGIIIVDSTRRGKTMPDALSKTIPIWCAVLNFIMFGEQEENGNNWCFLPLQIISKNEANSIIKLIPKFAQTLIDLKIINKEQLLTKFKGKYLRPLWMYPGMSPPIEPPIFEDFIPIINCVASFRADDGSKTMNGFTYVQGAADDHELWAENLNPQLFWENVDVLRNMELSDEQLLNIIDSLKIKKIGTSSLENVVELTDELSIGKVVDNIKLADVGKFDSIVVFSPNFTIEENESMKFHHYPFACDKKGSNELRKSIPSIIPSLSGRILVLCESGSDLSVGIALVLLSTRYNLDWEKVDTQPLTTKDTIKKHLAKVLNHKKVNPSRATLQAINSYLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUP2</accession>
    <name>A0A1E3NUP2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_59492</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FMO family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036106.1">
        <property type="nucleotide sequence ID" value="XM_019182838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUP2"/>
    <dbReference type="STRING" id="683960.A0A1E3NUP2"/>
    <dbReference type="GeneID" id="30200084"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00370">
        <property type="entry name" value="FMOXYGENASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ56899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="468" mass="53377" checksum="E718EF31AF8B372B" modified="2017-01-18" version="1">MNPSKIKDIAVIGAGPCGAGFTKALLAENAFSKIKVYERREKFGGLWNYTQLKSKCKEQVVKIPSINPNESVTKINAEGESFFESPVYKYLDANVPKDLMAYNNFPFDDNVPLFPRHDQILNYIERYSKEITDNVSFNEEVTSVKFIKEIKKWQVISNSLKNQEKTTELYDAVAIATGSYDKPMIPDVPGLKEWSEKYPSSVLHAKSYDEPAQFLKDGTILVVGNSASGADIAYQLATGLDRTIYKSIRSENPLPAGKDDRIKDVPDLSSFDPISKTVHFKDGSKLENVDRIIFATGYLKSIPFIKDEPLITDGQKVHGLYKHLIYYNNPTLAVIGLPRFVLPTRLAETQGCWLARIWSGRIELPPLEEMIKFDKSITCEDRKYHDLNYPKDVDYSNELNEQVKKAIGDYGYFAVHWDEEQTKIRSAIKQIKEGYIKYMKDKGRPAYSLKELEDAKYFEYPDPEAEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUR1</accession>
    <name>A0A1E3NUR1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_86288</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Spindle pole body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036073.1">
        <property type="nucleotide sequence ID" value="XM_019186033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUR1"/>
    <dbReference type="STRING" id="683960.A0A1E3NUR1"/>
    <dbReference type="GeneID" id="30203279"/>
    <dbReference type="OrthoDB" id="205662at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990498">
        <property type="term" value="C:mitotic spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099070">
        <property type="term" value="C:static microtubule bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061863">
        <property type="term" value="F:microtubule plus end polymerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030951">
        <property type="term" value="P:establishment or maintenance of microtubule cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990571">
        <property type="term" value="P:meiotic centromere clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000022">
        <property type="term" value="P:mitotic spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000019">
        <property type="entry name" value="Cytoskeleton-associated protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057546">
        <property type="entry name" value="HEAT_GCN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048492">
        <property type="entry name" value="Stu2_CTS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045110">
        <property type="entry name" value="XMAP215"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12609">
        <property type="entry name" value="MICROTUBULE ASSOCIATED PROTEIN XMAP215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23271">
        <property type="entry name" value="HEAT_GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21042">
        <property type="entry name" value="Stu2_CTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="7">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="7">
        <location>
            <begin position="279"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="232"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="512"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="728"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="830"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="630"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="693"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="247"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="512"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="551"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="604"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="736"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="761"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="800"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="837"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ56866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="850" mass="94008" checksum="ED1F307A26285A95" modified="2017-01-18" version="1">MSDEQPDYSKLPLEERLVHKVWKARLEAYQEITKDFEKSPNEESDVFTPFLSNPELFKKIVTDSNVVAQESGIAALNAFLEFGGPRGAMKLRSQVVTGLCEKGLSSSRAGTKQKTIDALLWLIELDIPDPVIEQMIPSLSARLPKLVSGTVKALADIYTAFGAQTISPKLVLPSLPKLFSHADRTVRAETSNLTVELYKWMGPALEQVLFAELKPVQQKDLAAAFEKVKDDKPTQTRLLRSQREAMESQTAQKESNNEGADDDIVMGDTEEAGQLDAYDLMDPVDVLSKMPTDLATKIAAPKWKDRLDVMNEVHQAINVIKLADDDYLELVRIFAKCLKDVNIQVASVAANCIDCLAKGLRKKFHKYYQIVLNPLLERSKEKKQSVLDALNGALDAVFESTSLSDILTDTLEAMKHKTPQVRLASSQYLIRCLKETKTAPTKPEIESIITTSIKLVGDTLPGVRTNGFEVVGTLMKITGERELNPYLEKVDDIKKNKIKEFYDVAEVKSKVTAPKPKAAAPSKPTSSTSSSKTPSKAASIKRKSLVAPKASSISKSTLGSTSSLQQKKKPLTGSSTIPSKRGPTSPLKNDPASTRIGALGNRGLTGRSLQSSTSSTLNNVPSIKQDSGLNDAERAELEDLRIQKQSWLKEREKLNWQVQESIAESSRLMTEIQTISNNSDRLNEQHTNDVMTIKSKETQLQRANSDLEISRLKISQLESEIMLLKKQRSSTMNTFSTSNGLDLSSSSSLENERPRQYAPLQESNGVRSSVNSEFSAKSPTLDNIDQKVGNLSIDNDQKENNGLSRTMLDTNDESWRRAAEVTSQLKARIEKMKAKSRNTNNIPSSSGYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVE6</accession>
    <name>A0A1E3NVE6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_97580</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036271.1">
        <property type="nucleotide sequence ID" value="XM_019186594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVE6"/>
    <dbReference type="STRING" id="683960.A0A1E3NVE6"/>
    <dbReference type="GeneID" id="30203840"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990145">
        <property type="term" value="P:maintenance of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002109">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000008">
        <property type="entry name" value="Ubiquitin-40S ribosomal protein S27a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002906">
        <property type="entry name" value="Ribosomal_eS31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038582">
        <property type="entry name" value="Ribosomal_eS31_euk-type_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01599">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01402">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="152" mass="17289" checksum="884E0F9A043E5157" modified="2017-01-18" version="1">MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGGKKRKKKVYTTPKKIKHKHKKVKLAVLTYYKVDKDGNVERLRRECPATTCGAGVFMANHGDRQYCGKCHLTFKAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVS3</accession>
    <name>A0A1E3NVS3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aldehyde dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_81433</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036421.1">
        <property type="nucleotide sequence ID" value="XM_019185232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVS3"/>
    <dbReference type="STRING" id="683960.A0A1E3NVS3"/>
    <dbReference type="GeneID" id="30202478"/>
    <dbReference type="OrthoDB" id="440325at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000004">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF16">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE TYPE III, ISOFORM Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="5">
        <location>
            <begin position="10"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR036492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ57214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="519" mass="58187" checksum="3B547D0147EC36D1" modified="2017-01-18" version="1">MSLPYTPLDQIPSIVSSLHESFHSHAKLDSIQYRLKQLRILYHAIHSNEDAICEAIKLDLNRPIQDTKFLEFDVFYSEILDIIANLESWSKPTPVDDVPKIISFASPKIERIPLGTVLIISPFNVPMLLALQPIVGALCGGNTMVLKQSEFVPNTALILSKILTRALDSDIFKVVNGAIDETTVLLEQKFDKILYTGSSQVGRIVAKAASKNLTPCLLELGGKTPVYVGASVEDSLEVVTKRIMFTKFINAGQVCLAADYLLVDEKIYDKFIKTLLKTVDEYFKGVDSKNFTHIIHERSWERLNSMIKSTKGQIIFGGKNSNRALKYIEPTIIKNVDFNDSTMKEEIFGPILPIIKISNIFETIDEIKSRHDTPLSMNIYSHDASEVHYLRTHIRSGALIINDGMMHVAIHSLPFGGIGESGYGAYHGIHSFKSFTHERAILEHPFWCDKFWTDKFIKIVDIENTWRGSKHLPQLPGKDVDFDANGEVIKKPIDEKVWKLGAVGVVVAGVVAYCYGLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVT9</accession>
    <name>A0A1E3NVT9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_65515</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036465.1">
        <property type="nucleotide sequence ID" value="XM_019183925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVT9"/>
    <dbReference type="GeneID" id="30201171"/>
    <dbReference type="OrthoDB" id="1919336at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF3">
        <property type="entry name" value="HALOTOLERANCE PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="18"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="825" mass="96358" checksum="1EE0E7E2A1389DBB" modified="2017-01-18" version="1">MQTPNFQIVEKKERKHTACLGCRIRRRKCDNVKPICSGCQEINIPADLCLYHGARVGFKVNGSKKTLEKLRDKKTQLLKEQAALTRVGQRRSQYQGYEAYYLPSYHNNQFKKNPLMCTMGDVSGTLTHYGPISWFALISVQEPLKKLSTWFINVMKFEKDRYNKSKFEKKVEEDNVLRTLNKLLIEFSLGCMEIPDKYSLLNQILSVIVSKLPIQSVSEELLKFYFHLNQQRAVGFVYLDKDSFYEKLEKYISFDDKGKAHLTMSLPKDQDGLSFIMLYLSMLTYQAFFASTLNTSSYPQVNHLQMLEYLETLLLASEVLQSDESFGISPFLDPETLLAIIHVCVFQRCTPHGNISRGIIGGNDVLIVRQLIVYARILRLDEDIDVAYQTKTPEYRKSLKSIWYCLMYFDISEGLETGIPLKIKPKELERYKNENLYIRSMIVINDVLNQFNDNVLMVDKLKLIEWIQNTLAPQLDNFLKKKFPSAEEHIEYLEKFDFDNINDSHKYVWHLQMAAIRILIYSTSASFYHFCATVTDQIGVESKKFRYIAMKRMGALVVFIRNVLNAVKILSEHKNSSYFSLVSSLLVISRQMTFALRRVSIYIGGMVVRNYDQQYSLQFLSTLMNDSDRYAKTLLDTLIKTSDYHSFSFSLDSLENFEIEKNFDELDKYYFRLDDYKFLAFENCRILQQMMKSLENKKYNLNFLRLDYIFFYGLTASNLFLALAFSSDEIKDNAKVAGKIPDAHDDNGNNIETEHQTEISDASRNNDFDFNDLFERAIDTNSERFDFKSFFTTSNDLYQHEDIEQFLENLTMPLSDYYSNNSPYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW48</accession>
    <name>A0A1E3NW48_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_46375</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036563.1">
        <property type="nucleotide sequence ID" value="XM_019182594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW48"/>
    <dbReference type="STRING" id="683960.A0A1E3NW48"/>
    <dbReference type="GeneID" id="30199840"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF281">
        <property type="entry name" value="BOX A-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="GATA-type" evidence="7">
        <location>
            <begin position="62"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="98" mass="11247" checksum="DFC268C726950819" modified="2017-01-18" version="1" fragment="single">MTKCANCEAEKTPMWRKTLDIETKAVLTLCNACGIYYKTKGCHRPKLLISKKNILSDLNIIRCSNCENINTSIWRRDDNGKSICNACGLFFKKKGYHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW76</accession>
    <name>A0A1E3NW76_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_36389</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036457.1">
        <property type="nucleotide sequence ID" value="XM_019182429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW76"/>
    <dbReference type="STRING" id="683960.A0A1E3NW76"/>
    <dbReference type="GeneID" id="30199675"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000935">
        <property type="term" value="C:division septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000196">
        <property type="term" value="P:cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050850">
        <property type="term" value="P:positive regulation of calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000263">
        <property type="entry name" value="MAP kinase skh1/pek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050915">
        <property type="entry name" value="MAP_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47448">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE DSOR1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47448:SF5">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINAE MKK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="36"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="319" mass="35697" checksum="2C5B2591B14CE6B0" modified="2017-01-18" version="1">MGNYEDEGEGTLQVQHKDVNELDEEGWRIVSKKGEIIELGVLGEGAGGSVSRCKLKHGSTVFALKTIITDPNPETQKQILRELQFNKSCKSPFIVKYYGMFLKEETASICISMEYMGGRSLDAIYKKVKLRGGRIGEKVLGKIADSVLKGLSYLHEHRIIHRDIKPQNILLDSDGNIKLCDFGVSGVVVNSLATTFTGTSYYMAPERIQGHPYSVTSDIWSLGLTLLEVAKGQFPFEMDTTNTSPIEVLTLIMTFTPRLEDEDGIVWSGAFRSFIDYSLKKNSSERPSPRQMLSHPWVVGQSKKSVNMGKFVRQCWDDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWN2</accession>
    <name>A0A1E3NWN2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Clathrin/coatomer adaptor adaptin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_35540</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036617.1">
        <property type="nucleotide sequence ID" value="XM_019182323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWN2"/>
    <dbReference type="STRING" id="683960.A0A1E3NWN2"/>
    <dbReference type="GeneID" id="30199569"/>
    <dbReference type="OrthoDB" id="10254310at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030123">
        <property type="term" value="C:AP-3 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="7">
        <location>
            <begin position="38"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="681"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="732"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="749"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="782"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="791" mass="89699" checksum="7ADAA49AF9A981C2" modified="2017-01-18" version="1">MSDSISRITSMLESARDLTLEAAAAASAKLAETPDHIRPKEVSTLLNSRSERDVLKGLKYVISLISSGEDASQYFTDVVKNVTSSSLKIRKLVYTYLLRYADKENDVALLSINAIQKSLGDKNDQVRALAIRVMSEIRISSIYHIVQLGIKKCASDPSPNVRKAAAISLVKVYTNHGHSSVQELTEHLKTLLKDRDVNVLSSAILSFRIICPENFDLIHGHFRRFCSIITELDEWAQIYLVELLTNYSRVFLPKPRIFNKATNESEFINLPDNYNEIPYPVYDVEFDSDLKLFLDSIKYLTYSRNEAVVLAVGRAFFHLSPPKTFKESQISASLVRILQTSIPETQVFILQSILYMAAHEPTLFVQYEKRFFLFPNDEILSAQYKLKILSIICNENNIKSITTELQYYALNSSDPRIAVESVKALGVCSQISQFWSAKTLKWLLSQITTLGQDRTVTAEFLTVIRYLVQQNPENNIKTVVKLAYLLDGDSLLESSAKESVIWLVGEFSGIEPKIGPDVLRKLVKGFAYEEPNVRYQIILLAAKIYSYDLENYKNSSGDLDLEKYGFDGIIPKLFSHVINLGKYDDVYDIRDRSRMLHSLLSSTTTHLATLILQAPKPVPLVTLRNVSDVSPDFIEFKDDDWRSLCIDDLVKNYNLLPPWSSKDLPDSSIRNAIEVVEAPKNFSQQTFPNQKSNSFKDPLTSFQPPVREKKYKLQSLDDFFAEDNSKPLRNSTVTKKKVVIQEESGSSSEDGESSDEEDSSSGEEESSEEEEVSDEEDEHDNNEDYIKKPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWP3</accession>
    <name>A0A1E3NWP3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Catalase</fullName>
            <ecNumber evidence="12">1.11.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_85728</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 13">Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2 H2O2 = O2 + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:20309"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="EC" id="1.11.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the catalase family.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.6" evidence="12"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036808.1">
        <property type="nucleotide sequence ID" value="XM_019185940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWP3"/>
    <dbReference type="STRING" id="683960.A0A1E3NWP3"/>
    <dbReference type="GeneID" id="30203186"/>
    <dbReference type="OrthoDB" id="6880011at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08157">
        <property type="entry name" value="catalase_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.180.10:FF:000001">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.180.10">
        <property type="entry name" value="Catalase core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018028">
        <property type="entry name" value="Catalase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024708">
        <property type="entry name" value="Catalase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024711">
        <property type="entry name" value="Catalase_clade1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011614">
        <property type="entry name" value="Catalase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002226">
        <property type="entry name" value="Catalase_haem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010582">
        <property type="entry name" value="Catalase_immune_responsive"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020835">
        <property type="entry name" value="Catalase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465:SF9">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00199">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06628">
        <property type="entry name" value="Catalase-rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038928">
        <property type="entry name" value="Catalase_clade1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00067">
        <property type="entry name" value="CATALASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01060">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56634">
        <property type="entry name" value="Heme-dependent catalase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00437">
        <property type="entry name" value="CATALASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00438">
        <property type="entry name" value="CATALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51402">
        <property type="entry name" value="CATALASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 12" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="7 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 12" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Catalase core" evidence="14">
        <location>
            <begin position="12"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038928-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038928-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ57601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="511" mass="57867" checksum="30C90032CBC659A9" modified="2017-01-18" version="1">MSGKDNKCPRVTTSQGFPLPNAFETQRVGEHGPLLLQDFNLIDSLAHFDRERIPERVVHAKGSGAYGYFEVTDDITDICGSAFLDTIGKRTRTMTRFSTVGGEQGSADAARDPRGFATKFYTEEGNLDWVYNNTPIFFLRDPIKFPHFIHTQKRNPQTHLKDPNMFWDYLCENQEAIHQFMILFSDRGTPADYRHMNGYSGHTYKWSNKKGEWFYVQVHMICDQGIKTLNNEEAGSLSGSNPDHAQEDLFSAIAKGDSPSWTCYVQTMTQEEASKLPFSVFDLTKVWPHKDFPLRRFGKLVLNENPQNYFAEVEQAAFSPSHTVPYQEASADPVLQSRLFSYPDTHRHRLGTNYAQIPVNCPYAKTFNPIIRDGAMTVNGNYGAAPNYLSTISNPVQYTPQPKVNYQQNQEVWTGPAQPFHWKVEGDIDYVQARALYKVLGKQPGQQRNLAHNISVHVAGAIPSVQDRVFELFAKVDSKLSNDIKKEVLELSPRDQSSSGKCPYKGANVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWS8</accession>
    <name>A0A1E3NWS8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable vacuolar protein sorting-associated protein 16 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_106973</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Essential for vacuolar protein sorting. Required for vacuole biogenesis, stability and to maintain vacuole morphology.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the VPS16 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036667.1">
        <property type="nucleotide sequence ID" value="XM_019180489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWS8"/>
    <dbReference type="STRING" id="683960.A0A1E3NWS8"/>
    <dbReference type="GeneID" id="30197735"/>
    <dbReference type="OrthoDB" id="1792at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.780">
        <property type="entry name" value="Vps16, C-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016534">
        <property type="entry name" value="VPS16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006925">
        <property type="entry name" value="Vps16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038132">
        <property type="entry name" value="Vps16_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006926">
        <property type="entry name" value="Vps16_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04840">
        <property type="entry name" value="Vps16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04841">
        <property type="entry name" value="Vps16_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007949">
        <property type="entry name" value="VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vps16 N-terminal" evidence="4">
        <location>
            <begin position="4"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Vps16 C-terminal" evidence="3">
        <location>
            <begin position="515"/>
            <end position="828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR007949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="851" mass="98806" checksum="3297E118C9C74F7A" modified="2017-01-18" version="1">MSNPSLGWERLRDVYYRNRELYSITWNIKNINNYIIAISKYTKAIASYKFQNDVNELKDQNTQDIEIYSGSGNFITSINWNNHVNGRIIDMKWTIDEKLCVLLQNGLIRVYYDFEGNFNEYEIGYDSQLVGIKSFLVHGNVLIILLNDFKIVYIDLDNTISVNSKLFKDLKIELPEESIHFWNWDVLPSSSSFQILISINKAIYAIPKYGKIIQKSIDGPFQGVHVSENHEFIALYQEGSKKIYIVTANFNKALFDFKLDSAPPPRQIKWCGSDAVAVAYDDYVKLIAPGGSLNFFINDDFKISSEIDGLTILTNVSLEFLSKVNNDTVDCFKIGSTSKSSILIDSIDKLNKHSPKANENLKIIGSDIFLNQAVQTCIRAAFEEFDPYWQKKLLKAASFGKSSLELNSSFKNSIKFVEACDQLRIINNIRSNEIGIFLTFTQFQQIGIEKLIKLLVKRQEFYQSFQISKFLKLPIDLIFIDWACCKIKYNPNLNDEELSQLIITKFQNINNNSYISFEKISTIAFQEGRLNLAKILINFESLFTKQIPLLLTMEEHELALKKSVESQDVDLILETILKLKETLSLPNFFKILNNSKIASNCFEYFHHDDIKLIHDYINQADRLIDIANFEIDQYIIHGDQEDYSKKLTVLQNSFDTYNLMRKNSNDSKQIEKQLKLVKLQEIYTNDFNIEFNNLSIVETLEKLILLNQNNKINQFLKDFKINDKKFQYIKLNFLIKEKKFDELFEWGMSLKKPIIGYEPFIQKLISQNENSMASKFLSKANLSYSDKIEYLIKLKDFKTVIEEAYHKKDLSTLDKIEPMISTPRLKELCNEYQNSLQQTSSATTTSTTRFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWX2</accession>
    <name>A0A1E3NWX2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TFIIS N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_97030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036843.1">
        <property type="nucleotide sequence ID" value="XM_019186554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWX2"/>
    <dbReference type="STRING" id="683960.A0A1E3NWX2"/>
    <dbReference type="GeneID" id="30203800"/>
    <dbReference type="OrthoDB" id="62853at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="37348" checksum="F5AAD80DCA9203E2" modified="2017-01-18" version="1">MLQDFRAESKAGTHMSSDDPVMEKAFSDAVAYQALNSKALEKLLRENNLLSKDEEYIEYESWLEEKKKPETVKKSGRKSSVAMSKESTEEPPRKKKVKQPTPEKKKPTFEELANRAVWFRSKLQRGLIQRRTDPTEAELSSISNVFKDLENFSDSINSDLLKVSKIHKVLKAITRVETLKRPDDYKFHDRSIDLILKWEPYIAELKSEKSNGDLNDTPNSTFEVHDHSSSPKKENGDKKEILKEEGNGNEAEKTLPKGEVKEESTDKSKETKEDSKQQINEKSKEEVKEESNELAKEALNEQEEKKEGGEAIAETDGNESNKPVESGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX40</accession>
    <name>A0A1E3NX40_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_102233</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036429.1">
        <property type="nucleotide sequence ID" value="XM_019180347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX40"/>
    <dbReference type="STRING" id="683960.A0A1E3NX40"/>
    <dbReference type="GeneID" id="30197593"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000006">
        <property type="entry name" value="General amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004762">
        <property type="entry name" value="Amino_acid_permease_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00913">
        <property type="entry name" value="2A0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF9">
        <property type="entry name" value="DICARBOXYLIC AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="370"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="400"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="442"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="472"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="34"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="552" mass="61355" checksum="3BB1F8B534F38618" modified="2017-01-18" version="1">MSYEKDNIDVEKFASPSEVVQEGYRLQQHLKARHVSMIAIGGALGTGLLIGSGSALSSSGPAPLFIGYTFIGFIIYIVMCCLGEMATYIPLPDGFSGYASRYCDPALGFAVGYSYLFKYLIVTPNQITAGALVLQYWVSRDKVNPGVWITIFIIVILLINIFGVKIFGEFEFWLSCLKVLIVLGLIILLFIIMLGGGPNHDRLGFRYWKDPGAFKPYKGIESVSKGKFVSFVNVLVTAVFAYSGTELVGITVAEAENPRKSIPKAIKLTFYRIVVFYICSVLLLGMCVPYNDPLLISATKAKTSANASPFVVAIKNAHIKGLDHLINACILIFIFSASNSDLYIGSRTLYGLACNGAAPKIFAKTNKYGIPYYALAVCTAFCALAYMSVSSSSQEVFGHFVNVVSIFGLLTWITILITYIYFSRAFKAQGISKSTFAYTAPMQSWAPYLALALCIIVAFIKNFTVFINGFNYKTFITGYIGLPVYVIAYFGYKIIKKSKIRRPEDVDLYTLKHYIDEEEELGKIKDAERKEQMKNGRKDATWFYEKLLGWLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX61</accession>
    <name>A0A1E3NX61_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_85867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036978.1">
        <property type="nucleotide sequence ID" value="XM_019185961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX61"/>
    <dbReference type="STRING" id="683960.A0A1E3NX61"/>
    <dbReference type="GeneID" id="30203207"/>
    <dbReference type="OrthoDB" id="10264707at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052742">
        <property type="entry name" value="Mito_N-acetyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43138">
        <property type="entry name" value="ACETYLTRANSFERASE, GNAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43138:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE ACA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="53"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="208" mass="23511" checksum="6D6F6E5F39253A97" modified="2017-01-18" version="1">MAPTSTGPLLRDPLGKITEPVIVELKDGSTGTIYPIYSVDDVPESLLRFMHKEMNDEIERGNTYPIYDIFSQKDFADYWFFSFVAIMIKGENPGLQEDTNWESEFLGTYYVKPNYPGRCSHICNAGFVVNPRIRGQSIGTTLGKNYLNWAPILGYTYSVFNLVFETNVASSKIWDNLGFERIGYVKKAAVLKGYDEPIGVIIYGKDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX65</accession>
    <name>A0A1E3NX65_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-histidine N-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_35260</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the methyltransferase superfamily. METTL18 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.85" evidence="3"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036996.1">
        <property type="nucleotide sequence ID" value="XM_019182288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX65"/>
    <dbReference type="STRING" id="683960.A0A1E3NX65"/>
    <dbReference type="GeneID" id="30199534"/>
    <dbReference type="OrthoDB" id="1723750at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018064">
        <property type="term" value="F:protein-L-histidine N-tele-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045903">
        <property type="term" value="P:positive regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF39">
        <property type="entry name" value="HISTIDINE PROTEIN METHYLTRANSFERASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="360" mass="40157" checksum="D16A6A2A86DAC6A1" modified="2017-01-18" version="1">MSFSFGFTQDNFSDDELIDAENQNSIAQVDVSQGVNPLDSSNKDAKPPIHEDFLKLQQSLINIRVTFERYTTPSGQVVFRRELFDVKHQLMSEEGGENQDEEFGVLMGTDTTDLQKNVYEGGLKSWECSIDAVDKLSTLSDSEVFGQGDVVELGCGTSLPSTFLFQRALKTGTTNLKFKLSDYNPVVLRLVTLPNLIVSWASTLPQEQLLELQRSEDPEIPILDDELQLTPALIQAFKETLVNKNISIELFSGAWNREFFNLITKDTNRIGLIITSETIYSPETLPIIGELLIELIMASKNSTGNIPTALVAAKDIYFGVGGSLIDFENYLQNKIQQGISIEFQTEKVKAGLQRSIVIIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXJ8</accession>
    <name>A0A1E3NXJ8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_80745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036609.1">
        <property type="nucleotide sequence ID" value="XM_019185116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXJ8"/>
    <dbReference type="GeneID" id="30202362"/>
    <dbReference type="OrthoDB" id="3980887at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="458"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="458"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="507"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="601" mass="68567" checksum="1216AEF039AC8870" modified="2017-01-18" version="1">MSTAYTRRVSFDSLNPSIQVEPDEPIHIPPQYSSRAKTTSSKRTLDLNFGYEPSSKHQDLYKVFDSFQEINNKGYKSNLSTTRRKIPKLPPPPNKSILKRSVDDVQDQGAAIPKSFNENDQQNSFASVLPSQQRKKSLVEMTAEELLAMDAQFRTGVNLDEFKFDGDNYLPFHNLSSIDSKKPKNGLPNIDKLVGNIVTVNNRKLLKTNSINFNSYTVSFRHRLMTKFIDQTYESFETDEEEDEEEDDERPESLRELIVYISGRRHTWDSINWVLKNFLVDGDHLVIISQLPPENNSNVDYKDELIDCDFIKSKADALSSYIFKAIELLNKQDLKISITIEFIKDSSVKNLIKNSINLYNPNFFIISSLISNRLSIRFENNHVKLPYYLMKNLNLSTIIIPDLLIEKNLISLDQNIPEINIETDDDDYITTSYFLHTLNQISDAYKSIELNQLKSDISLSSTSNSNSNSRSDSRSTNQANNQEYADTPTNYYPKIKFSDNLSIPKYNNSNPSNSSSPFSSRRNSNNSSGIYKVKSLLDDPIPNNIQRSKSYNSTPITNTISNVSNSEKKLPSNVVKKSKSIDEGSIKKSGFFSKFGFKKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXN4</accession>
    <name>A0A1E3NXN4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_85864</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036957.1">
        <property type="nucleotide sequence ID" value="XM_019185959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXN4"/>
    <dbReference type="STRING" id="683960.A0A1E3NXN4"/>
    <dbReference type="GeneID" id="30203205"/>
    <dbReference type="OrthoDB" id="3981238at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025928">
        <property type="entry name" value="Flocculin_t3_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13928">
        <property type="entry name" value="Flocculin_t3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="299"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="299"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="499" mass="49406" checksum="6783C327E81D6727" modified="2017-01-18" version="1">MSSSNPSSSGSLSAPYMTGFFQDGQPHWNIVVPGTLGPWSSVEVRASDDGTHYDLTDLQMLVDGVVVSGPSNLTKNSYDASYEDAIESSSNLVFSVFAQAIAGKNYTNSVEVAINTIDGKKLVKRAVLKFLLSATITTDASSIGSSSSAPSSGGSSSASETLSSGSASGVASDSVSMTSGGSFSLTTTVDQQSTTVITVTSCSDDKCSSVPITTGVTVVTSTVEGIVTTYTTYCPLTSVDHGSSAVHIEAPSTTVVTITSCSSHVCSKVTATTGVTVVTETNEGTETIYTTYCPLSSIASPENSSPGQSGNVPNASNSKGSTAANVSSTGGASNVPQGGKPESGAGSSPSKSGPNGNNAQSGTAISHGNTGTSSNAAAATAAAAGSNTGATAVGGGSASNAGTGAQGSYNSNTAIDSTSTQTVLATATVSYDQVSAGKIISSSEGAAVRGSAEGVRTLISSSTTLIPDISNTYEDHAVKKSAMASSAIGSFALCILLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXN6</accession>
    <name>A0A1E3NXN6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_35584</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036649.1">
        <property type="nucleotide sequence ID" value="XM_019182330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXN6"/>
    <dbReference type="STRING" id="683960.A0A1E3NXN6"/>
    <dbReference type="GeneID" id="30199576"/>
    <dbReference type="OrthoDB" id="432719at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02094">
        <property type="entry name" value="P-type_ATPase_Cu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000068">
        <property type="entry name" value="Copper resistance-associated P-type ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF32">
        <property type="entry name" value="COPPER RESISTANCE P-TYPE ATPASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="419"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="468"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="507"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="548"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="706"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="749"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1106"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1139"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="11">
        <location>
            <begin position="242"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ57442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1182" mass="129919" checksum="8FDD2C10D9ADFB16" modified="2017-01-18" version="1">MEVFNVTVSNIHCEDCRQSIITSLSDIFQISDHVPELNAPGDNPFAIVRIIDDAELNILAAKGSINSQLQKIVLAKLEGLGFIIKDYSLHDVQETQYLHTKYKTHQKLLSKYIPWLCHTVERKHLEHCTKCRDEKKTTSDADSELTKVVTMSPLEYRAVFTIGGMTCSACAQTVSQAFSESLVKTQNNEDDEAKFSVDPVSNTAIAIIPNKQIANNIIANVRESGYSATLVELLPVERETKYRVNAAIGGISCAACASSITNAVSNLSFVVESAISIVSKSGIFILDTDEESKLNELKDAIENCGFDFELVEIKKVNHATTKKQSRTVNLKVSGMFCEHCPEEINEILNSHGNAIVIDDPITLDHPYVKFTYIPSPPNLTIRSVLQEIKDAGKFEVEIIEAVSLDEHLRRIARLETLKIAYRLAITTVLAIPTFVFGIVGMSFLKKSNGFRQWLDEALWTGNVSRMTWILFFLSTPVYFFAADIFHIKAIKEIRTLWKKHVPWKRRLFRFGSMNLLMSLGTSVAYFASIALLGLAAHSQKMGHAFTTTYFDSVVFLTFFLLIGRLLEAFSKSKTAEAITNLGSLKPSSATLLAKLGENEYGDDVKTDLKLLEVGDFIRIVPGESPPVDCIIVQGKTDFDESALTGESIPVEHTVGEQIFAGTVNKGPQSIIAKLSALDGTSLLDHIVNTVRDGQMRRAPIERAADVLTGYFVPLITLLAIITWIVWLSLGYSGALPDSYLDIDIGGWAIWSLEFAISVFVIACPCGIGLAAPTALFVGAGLAAKNGILARGGGSAFQEGSRVNVVCFDKTGTLTMGGEPKVTNYAIYQDKVIKQFALQVAKDLEITSSHPLAIAVKAFIEEVSIDQNVKLGRVKVPEVEEIPGKGLKGDIIIDQDDSDAKNWNKFKPSKVLLGNESLMTESKAQFTSQQKRLLNEWKIQGKSVVIVGLQCFEFFKTDNFIPVLLMAARDEIRPEAKNVIKLLKNEGITTWMISGDNSITANAIAKELGIDNVVAEVLPEEKAEKIKWIKRTNYDNKKEPIVAMVGDGINDAPALSTADVGIALASGSDLALTSSDFVLLAPKHPLITLLTLLKLSKTVFNRVRFNFVWAIVYNCIGIPIAAGVIYPYKNSRLSPVWASAAMAASSISVVLSSLALRLFKKPKVVSEQSKNAELNQKALEEKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXP8</accession>
    <name>A0A1E3NXP8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pre-rRNA-processing protein RIX1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_80116</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA and the nucleoplasmic transit of the pre-60S ribosomal subunits. Regulates pre-60S association of the critical remodeling factor MDN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RIX1/PELP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037158.1">
        <property type="nucleotide sequence ID" value="XM_019185008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXP8"/>
    <dbReference type="STRING" id="683960.A0A1E3NXP8"/>
    <dbReference type="GeneID" id="30202254"/>
    <dbReference type="OrthoDB" id="20900at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012583">
        <property type="entry name" value="RIX1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34105">
        <property type="entry name" value="PROLINE-, GLUTAMIC ACID- AND LEUCINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34105:SF1">
        <property type="entry name" value="PROLINE-, GLUTAMIC ACID- AND LEUCINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08167">
        <property type="entry name" value="RIX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-rRNA-processing protein RIX1 N-terminal" evidence="7">
        <location>
            <begin position="9"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="468"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="679"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="754" mass="84561" checksum="0DB3A36FAD34EC52" modified="2017-01-18" version="1">MASVSAEIPLQVVLSLLDAPQPATLSYVLKSLYLSKYTITKASKADINHLIAKINNLLNSKDNFKKWYGSHVVQVIGLNPVILTNGGSTFIVGLLKTINDTSVPAVTFKNAALALDQLISGIRGKPVLTRELLTPNLPNIISSLLDNLNRDIYVVLPILKNLLLKNTTTFKPFVKKFETKLVDLLLKNFNSFNKQLKKDICQSYAYLNLVKPTQNTNQGGQNVQALPDDQWRAKIFKLLGELKSVIVIYDDLVELKNEKDSAELLNQLPNSDAKDFIFPFLKVDLSEPISIIQISQRIEVLTQLIIAFLTSPTPFPLRIPLGLIIQSSELLLGISPNYIPLKKELGRNEDLKKIIVNDISKTQAHGAFILYHITKHYKKLVLPHLSSVLSSLEVAIPIKNDKGSKVIKINDDSCLAIESELITIIKTTLEILKLFQGLTDLDLINKLIDVSILFLQKRTPLDGLLDKQEVKKSQQQQQGQKNKKKGKSKDSTPLADILSHAELFEVEPPKETISIILSFFEVVIKRIPTLSPNYRIKIVKYVISTAVKESSTKSHVLDRTKKLLEAVVLYPSNGEIYSILPIAKRLLSNNEVLSLLTNPRFPPLETKFKPQLQNSAIEDDVEDEEEETQELSNVPEVEEKPIEIGEVVEQNIKAREVPIPEQPSIVFKTDEEVKEQNVLTFADDKQPTEIEDESSSNKTRSLEDDEDTPSKRIKIVSEGTKTVQIPADKIGQEEDSDSDFEIPNIEVDSDEEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXU0</accession>
    <name>A0A1E3NXU0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA polymerase II transcription factor B subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">RNA polymerase II transcription factor B 38 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">RNA polymerase II transcription factor B p38 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_33648</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037183.1">
        <property type="nucleotide sequence ID" value="XM_019182097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXU0"/>
    <dbReference type="STRING" id="683960.A0A1E3NXU0"/>
    <dbReference type="GeneID" id="30199343"/>
    <dbReference type="OrthoDB" id="5963at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070985">
        <property type="term" value="C:transcription factor TFIIK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061575">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001174">
        <property type="term" value="P:transcriptional start site selection at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16573">
        <property type="entry name" value="RING-HC_TFB3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000037">
        <property type="entry name" value="Cdk-activating kinase assembly factor MAT1, centre"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004575">
        <property type="entry name" value="MAT1/Tfb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015877">
        <property type="entry name" value="MAT1_centre"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00570">
        <property type="entry name" value="cdk7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12683">
        <property type="entry name" value="CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12683:SF13">
        <property type="entry name" value="CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06391">
        <property type="entry name" value="MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17121">
        <property type="entry name" value="zf-C3HC4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="30"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="177"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ57976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="334" mass="39060" checksum="71D8003F1FCDD5AA" modified="2017-01-18" version="1">MAIELGVYDTRNTWALKTNISTEERQQDMCPICKTDRYLSPDMKFLVNPECYHKMCESCVDRIFALGPAPCPYPGCDKTLRKNKFKTQIFDDITVEREVDIRKRVSQIFNKTYEDFNDLDKYNEYLEEVETIIFNLVNKIDVEETEAKLSVYEESNKSKILENNNSREKEYEDFKKQEELEKELRLKKIMLEKQIEQEEKEMNEAARREILNKLSGSQDPTQMISQVKKSMLKKSSARRKQLDEIMKSLNSKQSGNEQQDTNSVPFTPFNGDRVLAKDFSISQEYYDPFIDELQKKKDYIAAGFKAEQVYERVLSEAFIGLGCFIEEEKASAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXX5</accession>
    <name>A0A1E3NXX5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_80219</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037266.1">
        <property type="nucleotide sequence ID" value="XM_019185020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXX5"/>
    <dbReference type="GeneID" id="30202266"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="373" mass="42416" checksum="3D3EAF859836C95A" modified="2017-01-18" version="1">MCAHYAFRDKNGFSIQSTSMLLKRPCGLGYDTATSWCFEGMRFIRLDEGGFQKRDTDCKPFRILDSTPRSLGLIHYFEPPSIFKDQGFLLDDEMIKCPIPKNLPNFQEYVIHHGRTFIEENSANVARNHWRPGADVVDNTENNPKQLAGFEVAYYRLVNVDVQTSILKILNGLRMNNALATGTTTFERLDDFTDPTLSFEKFQKHGFCLQVESTGKLAITFERVEKLLLFMIEDLFAKDHNEEYIDKKVAEQRASIAFCRLKCRKGAPETTCPITIVKHLTASLRDLLWNLTKTLMHNDDMILSDIGSRNIYTGRVISKKKLTLYMYPDLVLNSARNSVITEPSEATSVTSNAPLLVSPFVPPPSYEESLNNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXZ2</accession>
    <name>A0A1E3NXZ2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyltransferase family 32 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_80098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037141.1">
        <property type="nucleotide sequence ID" value="XM_019185002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXZ2"/>
    <dbReference type="STRING" id="683960.A0A1E3NXZ2"/>
    <dbReference type="GeneID" id="30202248"/>
    <dbReference type="OrthoDB" id="409543at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039367">
        <property type="entry name" value="Och1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834:SF1">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="373" mass="43550" checksum="0C47CE06CA254DDD" modified="2017-01-18" version="1">MQLYKTKFIPLAAILTIVLINLGLYKFHAPTNQTIDELWVKYTFKSVDQFRISQIYKPDDLRLKLKTNDVSTLDQGLQILIKTWDDQDGFIHKIIPNHENDQVVDDIFINEIPEISKAFKKMPLVILQSDLIRYLILFAKGGVYSDIDTSLKSNLSNWLSYNNTMIQDPSNNKIGLTIGIESDRDDKAWIVYMPRRLQFCQWTLQAKPGHPFFRELIYRIADLSLNHYDETTKILTKNGKKFDFNDGSSTKYDGVMEWTGPAMFTDTVFDYLNEVYKTTEKLNPGVNFKQDNIINRNPLSLQKKIKYAKPKDNGHMSSYDPIHRPIGWQNLTLLQYPILFDEDVLLLPPAYLADKEHDPRQYVFHHFKGSWKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY77</accession>
    <name>A0A1E3NY77_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquinol-cytochrome c chaperone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_80213</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBP3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037260.1">
        <property type="nucleotide sequence ID" value="XM_019185019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY77"/>
    <dbReference type="STRING" id="683960.A0A1E3NY77"/>
    <dbReference type="GeneID" id="30202265"/>
    <dbReference type="OrthoDB" id="10253878at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061671">
        <property type="term" value="C:Cbp3p-Cbp6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005761">
        <property type="term" value="C:mitochondrial ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070131">
        <property type="term" value="P:positive regulation of mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021150">
        <property type="entry name" value="Ubiq_cyt_c_chap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007129">
        <property type="entry name" value="Ubiqinol_cyt_c_chaperone_CPB3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12184">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX ASSEMBLY FACTOR 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12184:SF1">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME-C REDUCTASE COMPLEX ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03981">
        <property type="entry name" value="Ubiq_cyt_C_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquinol-cytochrome c chaperone" evidence="3">
        <location>
            <begin position="140"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="329" mass="38138" checksum="10EC2C1057D484B5" modified="2017-01-18" version="1">MLRVPTICKAYAVPAARIATRSYATSRIPQPKDVKKIDDRSPSQIATSSTLNIEIPENKAPVKPKTKRPMLSDSKYEKEGYELSGFMKNIGESVISILRLDMDKIRAGPIAGSVYYAECKKQGLQFENEELSESAAFFYEDLKLPRTFSQWFQITALHQWMLSVRMRAMPFKYGRNYQQKLVDRFFRDMELRLNEEMNVNSSRITDQYLKDYNSQLRGLVCSYDEAFVTDDVTLAQALWRNLFNAQKNVDMMHLEAMVSYVRGQLYVLSKMSDRDFAFGKFKFVSPNEVVKRFTAEQEAEILKKAKEEYEPKEGKKILPSERSSLSYEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY87</accession>
    <name>A0A1E3NY87_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_80224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037270.1">
        <property type="nucleotide sequence ID" value="XM_019185022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY87"/>
    <dbReference type="STRING" id="683960.A0A1E3NY87"/>
    <dbReference type="GeneID" id="30202268"/>
    <dbReference type="OrthoDB" id="3980263at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20558">
        <property type="entry name" value="CYCLIN_ScPCL7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF32">
        <property type="entry name" value="PROTEIN KINASE COMPLEX COMPONENT, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G07660)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="263" mass="29881" checksum="21AC135D5DFF042B" modified="2017-01-18" version="1">MDTFRDAPAEPPSATLMSPSQSKIADIDVDEFFQQDDFNLTEVDSMMALTILNYLIRLLVIRCQDECESPVDALSDLPSPPLGPEEIDDCPLSPRKTNSRSSDSQGETQFLENTPDVNESNKNPTSKLMQNKILAKRFSLKSNPPVTVEEYLERINQYCGLSTAVYLTTSLYLFKLVRRNEVLKLNDLNIHRILIAALRISCKTVEDMNHRQTFIAKIGGVNNKDLLSLEISFLFLLKFECQANETILTTFLRDMKILHQESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYA7</accession>
    <name>A0A1E3NYA7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_65028</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036889.1">
        <property type="nucleotide sequence ID" value="XM_019183850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYA7"/>
    <dbReference type="GeneID" id="30201096"/>
    <dbReference type="OrthoDB" id="10519086at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="317" mass="35117" checksum="60A2902D3E531D4C" modified="2017-01-18" version="1">MTAPGANPIYQFSLGRKPTLRLCSGSPRLSVFERQLSLMKTPTIMQNNLLNSPNSGNRSILTNSQGSSPISDLQSSSNSSEATLIASGNNLDNLNPMKYIGINSSKSSIPSNSPVPFKKQNSIQRHKETSSTNKSIAFKIPIQEIINYKNSLEHFINEGLDLVYSITRNDVDLFLDYLSELVIGVFVVFGAEILKLNKDGFLVLVQFVTASVIRIGFKNGEYDSLLLQSPIVPILEGSNLPVEFKRSVSHLITTLQFYILFSTLRITAIFKNNYCIEDSRIDSLSIMGLGAVMILLCLNLIINSLPASRRYNSELEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYE9</accession>
    <name>A0A1E3NYE9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">type I protein arginine methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.319</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_85162</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:48101"/>
        </physiologicalReaction>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="1"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037227.1">
        <property type="nucleotide sequence ID" value="XM_019185851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYE9"/>
    <dbReference type="STRING" id="683960.A0A1E3NYE9"/>
    <dbReference type="GeneID" id="30203097"/>
    <dbReference type="OrthoDB" id="7848332at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035242">
        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035241">
        <property type="term" value="F:protein-arginine omega-N monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060567">
        <property type="term" value="P:negative regulation of termination of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.160.11:FF:000001">
        <property type="entry name" value="Blast:Protein arginine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000003">
        <property type="entry name" value="Blast:Protein arginine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF53">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="7">
        <location>
            <begin position="58"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="8">
        <location>
            <begin position="160"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="341" mass="39343" checksum="40E77E9925616E03" modified="2017-01-18" version="1">MASDSATDKASLPFSEQHYFSSYDHFGIHEEMLKDQVRTLSYRQAILKNKSLFKDKIVLDVGCGTGILSLFAAQAGAKHVIAVDMSNIIEMAQKIVDLNGYSDKITLVRGKLEDVKLPYPEVDIIISEWMGYFLLYESMLDTVLYARDHFLKKGGLIFPDKATMRIAAIEDGHYKDDKIHYWENVYGFDYSPFIDIAMCEPLVDIVENTAVATTSALLTEIDINTVKIEDLAFFTKFKLKAVRDDQIHGLVAWFDIWFPCDEEKNRVYFSTGPHANYTHWKQTVVYLDQVLDVKKDEEIEFTLANRPNAVNPRELDIEVNWDLKVDDEYRSTKGKYNYFMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYK8</accession>
    <name>A0A1E3NYK8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_85327</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019185878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYK8"/>
    <dbReference type="STRING" id="683960.A0A1E3NYK8"/>
    <dbReference type="GeneID" id="30203124"/>
    <dbReference type="OrthoDB" id="1284551at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045159">
        <property type="entry name" value="DCAF7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19919">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="180"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="287"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="325"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="325"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ58256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="393" mass="43150" checksum="32D6014EC47C65B2" modified="2017-01-18" version="1">MYLCKIHIQIVLGLCYTFHNGLKPWHDSTGTETVALSTYREDSINKIEILDGSSIMIEHANGVTSESVNFTKISEANVQLPVTKLQWDPSGTERLITTNDRLKIWEIDYNNQLVERLNLVNGVQHGHNGHSGTTNKPLSLPPLTSFDWNKVKPNIVITSSIDTTCTVWDILHPSSPKTQLIAHDSEVFDVQFIKNSAEIFASVGNDGSMRVFDLRSLEHSTIIYEPPVIPGVSSTTSLPSSTTGPSNALLRLATSNVDPNVIATFAAKSESIIILDMRYPGVPTTVLNGHNGPINSIKWHPTQQKLLSGGDDCQALIWDLTSAKPHPHLHHHHHQQQNSSNSNSAPKNGGGVVEYPDFAYNDTLEINNVTWNSEGDWIGVVSGKGFQGVKTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYR3</accession>
    <name>A0A1E3NYR3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_85389</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037533.1">
        <property type="nucleotide sequence ID" value="XM_019185885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYR3"/>
    <dbReference type="STRING" id="683960.A0A1E3NYR3"/>
    <dbReference type="GeneID" id="30203131"/>
    <dbReference type="OrthoDB" id="5215911at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF2">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G08910)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="357"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="432"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="467"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="498"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="80"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="522" mass="58000" checksum="73F16B6FAD89D93C" modified="2017-01-18" version="1">MQSGESIEKPAVDHADWADYNSAESESAANHHEIIFHRKHITPEHEAFLLKHHGRTDLNPLPSQDLNDPLNWPEWRKNAQLALVAFHSFSTTFIASGVIPAYSNFAEDYGVSLHACTYFTSCQILMLGCIPIFFTPIMVKYGQIKIFLISTLGSCVFNIGCIFCKTYGAQLACRIMCAVFISPGVAMGGTIVHDVTFAHERAAKTGWWSLLYTLGVPAGPLFMGFVVTQTGHWQYMFVVFAATNFIQFLVYLFAGDETAYDRSKVQTDEVIEAKGYWSNLVTKLYKSHNYHPEMNLLSLKVYLHPIILSIKNYKVSVAAVSYGIIFGYSNVACSVELPSVFVTKFGFDAQQIGLQNIAVIIGSFIGEIIGGKLSDIFMAYCVKKRSGGDKEIVHKKIEDRFWIAYFGYVMAVVGLIVFGVQTQNARQDHWNVTPLVGLCLTSLGNQVVTTTMITYAIDSDTANATHISLFIVWLRQVIGFVGPFYFTAMFNNLGFATSFGIMAAIEGFVALGIVIVTHIINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYR8</accession>
    <name>A0A1E3NYR8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carbohydrate kinase PfkB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_14781</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037059.1">
        <property type="nucleotide sequence ID" value="XM_019180620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYR8"/>
    <dbReference type="STRING" id="683960.A0A1E3NYR8"/>
    <dbReference type="GeneID" id="30197866"/>
    <dbReference type="OrthoDB" id="198885at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004730">
        <property type="term" value="F:pseudouridylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1790.10">
        <property type="entry name" value="Indigoidine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01876">
        <property type="entry name" value="PsiMP_glycosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022830">
        <property type="entry name" value="Indigdn_synthA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007342">
        <property type="entry name" value="PsuG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42909:SF1">
        <property type="entry name" value="CARBOHYDRATE KINASE PFKB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42909">
        <property type="entry name" value="ZGC:136858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04227">
        <property type="entry name" value="Indigoidine_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110581">
        <property type="entry name" value="Indigoidine synthase A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="6">
        <location>
            <begin position="325"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="662" mass="71392" checksum="59082936E58C29E7" modified="2017-01-18" version="1" fragment="single">FSSLKLSEELKWALQEKKPIVSLESTIITHGLPYPQNLQMAKRVEQEIRTHGAIPATTAFINGVPKVGLTDFELEMLANSSNATKVSRRDIPYVMSQRLNGGTTISGTMILSNRAGIKVFATGGLGGVHRDGENTMDISADLDELGKTPVAVVCAGPKSILDIERTMEYLETKGVFVGTYGPEGTNVPGFYTRDSGVASVYNFQDFQMAANIIKQGESLQLNSGLLFCIPPPKEIAMDGEFINKIIDQANYDAVKLGIKGKKITPFMLSRIAEATKGESVKTNIEFVLNNARAATNIAIEYSKLNGGNVDSLNPYETKPSTTAQSMVIGSVALDTSCLSNSESIMLNDSNPSTIKSSIGGVGFNVAMESIKSGNDSLKFVSSVGDDAQGDKILKSIDMNHAIEVQHNSSTSQYISFHDGKGDLVVAAADMGIIETISNDHIEKQLKDTKPVVLLMDANISSSSMQKIMNLSQDHSFKVIFEPTSSPKAKRLSEVELKPFPECSILLSTPTFAELRSIYDSFEESGKFELDNWFPIIDALGVDKTLKHKVESASMRNPFYKSIIKDGILQTGVSLLPFIKHLVIKDGANGVFHLSIHEDIQQITPNPAADLSLSSKGQNNLGVLLEHYSVPSVLDNLSNVTGAGDALAGSLLSALSVNSDVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYT6</accession>
    <name>A0A1E3NYT6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histone deacetylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_16617</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037563.1">
        <property type="nucleotide sequence ID" value="XM_019180701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYT6"/>
    <dbReference type="STRING" id="683960.A0A1E3NYT6"/>
    <dbReference type="GeneID" id="30197947"/>
    <dbReference type="OrthoDB" id="5232919at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053244">
        <property type="entry name" value="HDAC_HD_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47558">
        <property type="entry name" value="HISTONE DEACETYLASE HOS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47558:SF1">
        <property type="entry name" value="HISTONE DEACETYLASE HOS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="1">
        <location>
            <begin position="62"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Histone deacetylase" evidence="1">
        <location>
            <begin position="361"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="490" mass="54228" checksum="75BB6782379BF4E4" modified="2017-01-18" version="1" fragment="single">DSLENFKSQFIEFLSNNPHIVTNSKFVPSEEKALIILSPLSVKHSFGRTWVTKSYVSTIVERPQRLLACSLGIAAALSMYPSNYTLLSSTKKSPLHSPHVLKIHGKNWPADLEKICKESEAKLAKGEVEVPADWNSGDIYLTKDTLDAISGVIGSVETAVDHIFKQDGETPKRAFVAIRPPGHHSHPCVPSGFCLLNNAQIAIEYAASSHDVTHAVILDIDLHHGDGTQDICWQKAGFEPDFGESAAGYVDEEDDGIKKEANDPKVGYFSLHDVNSFPTELGYATSGNIKNASTCIMAHDLAIWNVHLQPYDKEEDFYIAYQEKYTQLLKKADEYLSNAKLDYERSKSKSHGKEPHPPFKALVVISAGFDASEYEVHTMQRHGVHVPTSFYSKFTSDAVKLANKHSNGVLLSLLEGGYSDGALTSGVFSHLIGLQCQKWDHGWGNQEVVKELVKGCKPKWTPLKTPNTDIKQWANQVCKLGRSMLPEAII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ22</accession>
    <name>A0A1E3NZ22_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_85470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037643.1">
        <property type="nucleotide sequence ID" value="XM_019185894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ22"/>
    <dbReference type="GeneID" id="30203140"/>
    <dbReference type="OrthoDB" id="3997851at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="100" mass="11453" checksum="6A864D6D638C0466" modified="2017-01-18" version="1">MQGVKTKSGLPQPYNMKPKNKGFLTTPMKKLIAYIFVLLIFGYIIYEISPKPEKEDPKYELDSQARVQENQKSAKNVIDKEKLGDIDLADAHMDSNHKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ37</accession>
    <name>A0A1E3NZ37_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_70275</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019184198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ37"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ37"/>
    <dbReference type="GeneID" id="30201444"/>
    <dbReference type="OrthoDB" id="2962993at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000013">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF9">
        <property type="entry name" value="MAJOR FACILITATOR-TYPE TRANSPORTER HXNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="483" mass="54250" checksum="21A2A33EE50906ED" modified="2017-01-18" version="1">MSLDKELGVTAVKSSSASAEEGALTESYYIDPALQRKYVREVDFKILPVIAVMYFCSAMDRSNISNAFSDGMTKDLNFEGQEYSLLLLLFYIPAGIFDLPFNILTKKYGAKWVLPSLMISWGVLTMITAACKNFAGTLVVRLLIATAESGFFSGCVFYMTLFYTRVELAFRIALFVTASVLAAAFTGLLAYAVFQIESTALHGAATVAISLFAYWWLPSTPSTCRWFSPEIKEVARLRHLKNGSNQVDSKFSWKECKERIMTWQWWVYFCISFTYPLPWTTASLFLTQIIGRFGFHTVKTNLWTVAPNCVGAVAVLCIAYSSDHFRERSYHLCFVLSLSLVGLIILATVDGYEHKAVSYFACFLLCCGAYCPSFLVHSYHNNNNLSESSRAFDSGVFVGLSNLASITASATFRTEYAPNYTPTIIATCVSAFICFCLTFFLGTWQKLENKRRNKEQGVELRAEDVDTNQLVNGTADPRWRYFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ39</accession>
    <name>A0A1E3NZ39_WICAA</name>
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        <recommendedName>
            <fullName evidence="2">Pyrroline-5-carboxylate reductase catalytic N-terminal domain-containing protein</fullName>
        </recommendedName>
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    </gene>
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        <name type="common">Yeast</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Phaffomycetales</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_019037179.1">
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    <dbReference type="AlphaFoldDB" id="A0A1E3NZ39"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ39"/>
    <dbReference type="GeneID" id="30202257"/>
    <dbReference type="OrthoDB" id="550646at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
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    </dbReference>
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        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
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        <property type="entry name" value="STEAP_metalloreductase"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="4"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="271" mass="29504" checksum="C368EE150D2E8157" modified="2017-01-18" version="1">MSETIGFIGVGQIGGGVAQLAVDAGYKVALSNSRGPESLKDVVNKLGPLATATTSEAIAKDSNIKYVVLSVPLVIVPTLLPKINLENKIILDTSNYYAQREGKIESLEDHTLTTCEYVSQYLDKSNKLVKVFNNIDSVHLLVDATKEVSKQTTLPIAGDDSDAKAAVTKILNNIGFQALDYGLLKDSWRSEPGEPIYGIPYMPVTPEGLEKAAEKKYFLETPAEPLSKEAAQKFIDDAKVLTPVGGSIKDFPKIWIEIIMEKYAQQQQSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ75</accession>
    <name>A0A1E3NZ75_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_64497</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_019183749.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3NZ75"/>
    <dbReference type="GeneID" id="30200995"/>
    <dbReference type="OrthoDB" id="444255at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="5">
        <location>
            <begin position="294"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="585" mass="68584" checksum="A39680207DDF2A44" modified="2017-01-18" version="1">MNEPSLRGDSVKQLSSKDAKAFGYKLEDTLAIYISYLKVCLENGNFNPVLPFSWSDWYQLNYNYELGDIQDVTNNFNFEVAESQEIKAKRGLEYIQFLNYSIERLIFMDSESTRNFVVRVDNDSSGKSSVRDSVSGLLVYLQSKGIYSTPINYQSDLRLISRIQQNNERFLELPKFETRSNMDYDSFDGDEMFINLHEFDFDFNLTQRTEELRNFVSDRNSNELVKLYYNNIHFVEMLAETGNFEKFFHEVELTEDPSLAGSHYDWRFFKHVLQEKQKYFVLHHLIRTWQEFANKEKLIYWLSHGNLLSWRWSGSSFIWDHDCDIQLPIKHLEFLAINFNGSLIIEDPKYGFNRYLIEVNPYFADRLNVDGKNSIDGRIIDLASGLYIDLTGMSTIDDDTGIISDKHIHRYSLQSLSPLRATLYEGKQTWVPHDVDYILTQEYPTGLFDTQYHDYQYLPNFKGWFKNPDDCLEKSLLSVRAFHIPSVDSRDHCIDFTFAQLNHLMKFWNLKAIEEELLCFNKIHCKLPLDQEGLNSIENSTYIEASSNIQKWDLDDFAAEGLRKLVNSFTLGPIPSTDMTVNCFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZC0</accession>
    <name>A0A1E3NZC0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_85542</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037746.1">
        <property type="nucleotide sequence ID" value="XM_019185906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZC0"/>
    <dbReference type="STRING" id="683960.A0A1E3NZC0"/>
    <dbReference type="GeneID" id="30203152"/>
    <dbReference type="OrthoDB" id="7722975at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019677">
        <property type="term" value="P:NAD+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07407">
        <property type="entry name" value="MPP_YHR202W_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000043">
        <property type="entry name" value="Ser/Thr protein phosphatase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.780.10">
        <property type="entry name" value="5'-Nucleotidase, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036907">
        <property type="entry name" value="5'-Nucleotdase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006179">
        <property type="entry name" value="5_nucleotidase/apyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053828">
        <property type="entry name" value="Nucleosidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014485">
        <property type="entry name" value="Pesterase_C1039"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041823">
        <property type="entry name" value="YHR202W_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575">
        <property type="entry name" value="5'-NUCLEOTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575:SF22">
        <property type="entry name" value="ADL392WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21953">
        <property type="entry name" value="NadN_nucleosid_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017316">
        <property type="entry name" value="Pesterase_C1039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55816">
        <property type="entry name" value="5'-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="28"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Putative 5'-nucleotidase C-terminal" evidence="2">
        <location>
            <begin position="350"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="586" mass="68146" checksum="249A10C247FAED15" modified="2017-01-18" version="1">MHLSWILQPYISPPKNSYFRELVWGDLNFIHTTDTHGWYAGHLNQRAYSADWGDFISFTENLHELAKKRHADILLVDTGDKHDGNGLSDATNPNGDLSTPIFNEQNYDLVTLGNHELYQEDYSTQEFQITVKNFGEKYVSSNVEYKLGNEEWVPFGNKFRYFETTNNKYKVLALSFLFNFPRYNSKTRVFPIKEAIQQDWFKNLTSRYSEDDIDVLVVFGHIPVTGFEEEEIIHLHVALRRLYPNLVIQYFGGHSHIRDYAVYDHKATGLQSGRYCETAGWASLNFTGEYKLKPKFSRRYIDFNRESFIHHSNSDKDNFDTEKGLSISKKIRKTRLDLNLTVSHGYVPRNYLMNEHPYGHPSNLYTLLNSTILPLLESSIDRQTEKYNRIILINSGSIRYDLYKGNFTTDTEFTISPFQNDWNYIKLPKALALRLKHYLNQQEFFIKSGTDLAGPEKRGVLNKMAREKQFKSLTTSDRLSCSFVKDPNFRKGQTTSDDHGCDGDDVFHNSVLYYPSPNVVQTYQEFNKESDEVDVVFYSFIKPYIISAINALNNHNGKNFTNDDVEYYGGESTGSLLRTYIKENWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZV4</accession>
    <name>A0A1E3NZV4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamine-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="8">6.3.5.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">NAD(+) synthase [glutamine-hydrolyzing]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_31601</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>deamido-NAD(+) + L-glutamine + ATP + H2O = L-glutamate + AMP + diphosphate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">In the C-terminal section; belongs to the NAD synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.1" evidence="8"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037948.1">
        <property type="nucleotide sequence ID" value="XM_019181856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZV4"/>
    <dbReference type="STRING" id="683960.A0A1E3NZV4"/>
    <dbReference type="GeneID" id="30199102"/>
    <dbReference type="OrthoDB" id="2020662at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00334"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07570">
        <property type="entry name" value="GAT_Gln-NAD-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000036">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.110.10:FF:000003">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02090">
        <property type="entry name" value="NadE_glutamine_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014445">
        <property type="entry name" value="Gln-dep_NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090:SF9">
        <property type="entry name" value="GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006630">
        <property type="entry name" value="NADS_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8" id="KW-0520">NAD</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="9">
        <location>
            <begin position="5"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="715" mass="80420" checksum="450FE1F03F90C114" modified="2017-01-18" version="1">MGHLITVATCNLNQWALDFEGNRDRIVESIRIAKERGATLRVGPELEITGYGCLDHFLENDLYLHSWEMYASILTNKSTHGILLDIGMPVVHKNVKYNCRILSYDGKILFIRPKIWLANDGNYREMRFFTPWQRPRHVENYVLPRMIQKITGQESVDFGDAVISTLDTVIGAETCEELFTPNAPHVGMSLDGVEIITNSSGSHHELRKLNTRMNLIKEATTKCGGVYLYANQKGCDGDRLYYDGCALIAVNGEVVAQGSQFSLEDVEVVSATIDLEDVRSYRSSISRGLQATSNQAYKRIKVPIELSPTSMAFDPKIAPTKAQEIFYHTPEEEIALGPACWLWDYVRRCNGAGFFLPLSGGIDSCATAVIVHSMCRLVAQAAESGNEQVISDAKKVANVKDEEAKDWKPTDPVDFASRIFHTSYMGTVNSSKETRSRAKELSEKIGSYHVDLNMDTLVAAVVNVFEVATGKKPIFKILGGSQTENLALQNIQARLRMVLAYLFAQLLPWVRGRQTGGLLVLGSANVDECLRGYLTKYDCSSADINPIGGISKTDLKKFIAYARDEFGLPVLQEFLEATPTAELEPITKDYVQSDEADMGMTYEELSVFGRLRKVQKCGPYSMFLKLYHEWTPRLTPSQVAEKVKKFFFFYAINRHKQTVLTPSYHAEQYSPDDNRFDLRPFLINPRFAWASKKIDEIVKQSEKGQTEDLEELTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZW5</accession>
    <name>A0A1E3NZW5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_79193</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037852.1">
        <property type="nucleotide sequence ID" value="XM_019184851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZW5"/>
    <dbReference type="GeneID" id="30202097"/>
    <dbReference type="OrthoDB" id="10620288at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="244"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="413" mass="46664" checksum="314E2F00C9C1F0E0" modified="2017-01-18" version="1">MEPGPVVDINKLRYLTKSLPQSPRLRNSIDFSDFAAKNFEKSTGVTQPPKKEHVRKQSKWVYNEVQRNSINFGISVEDMIAYHQQKKFEQLRNEDVQHGSVNVGQNKENHDPFNEQPALKSPFAIPKNSPQFERAENLPRIPRIAPPRPKTRPPTLNIGTPSGIEFASANRTPPAPPEEKKETEIGTPTRPQQHHMEDSQALTRSSSSKILKRKSSASSKHNSFIDTGDFKSKLSPSNLRRRSSQVSSRSSLNSRPNSIPTQRECHSVEETLLADEEFNDYTVPSLKSRTSSYFAKTNEFNFEKPLSRTSSLGSFRSPDLNRSSSSQFKTLPTSPKTPTRQPRQRHSVSDFQRTDESSPGLRSTRSFSTNDTFGSSPVRYSPRMSLRTSMSDLKASPIEKISEHEILHFGSPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZY5</accession>
    <name>A0A1E3NZY5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_93134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037872.1">
        <property type="nucleotide sequence ID" value="XM_019186403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZY5"/>
    <dbReference type="GeneID" id="30203649"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="293" mass="34200" checksum="AA924DAFD93080D4" modified="2017-01-18" version="1" fragment="single">MELARPQILNPKNPAPYTTGEPVAEENEGMLHCLKIKRITQINYAQDIFSSFASKDISPKHVHGNMFISVRLIQSLCPYWYKLFIVIPSKLVTILRKPIITVPRFVLFPPKSPVNEDVDMDEVSHPQRDPNPVSYQMRAFDELTIQFNENDVYDYEKELNAIDKVIFEKVSKGKAVCPFAIYTNLFEVKYHPTPYSNFYQIPEDDLEEAFKSVVQKFDWFFDTCLKYKISILPEQFLIPTSHPRFSSLGSDLKDCLNHTQPPSLVYCCNNNFMLFEFTQDDLENMMKSYFDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZZ1</accession>
    <name>A0A1E3NZZ1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dipeptidase</fullName>
            <ecNumber evidence="1">3.4.13.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_79107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an L-aminoacyl-L-amino acid + H2O = 2 an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:48940"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="ChEBI" id="CHEBI:77460"/>
            <dbReference type="EC" id="3.4.13.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. Peptidase M19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.13.19" evidence="1"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037767.1">
        <property type="nucleotide sequence ID" value="XM_019184841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZZ1"/>
    <dbReference type="STRING" id="683960.A0A1E3NZZ1"/>
    <dbReference type="GeneID" id="30202087"/>
    <dbReference type="OrthoDB" id="445695at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070573">
        <property type="term" value="F:metallodipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01301">
        <property type="entry name" value="rDP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008257">
        <property type="entry name" value="Pept_M19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10443:SF12">
        <property type="entry name" value="DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10443">
        <property type="entry name" value="MICROSOMAL DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01244">
        <property type="entry name" value="Peptidase_M19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51365">
        <property type="entry name" value="RENAL_DIPEPTIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU341113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="412" mass="46914" checksum="45482B5F3AC5AD89" modified="2017-01-18" version="1">MTVEDIQKRFEALAAKVPIVDTHNDFPYTLRVQLHNEIYNDPNFDFDKILTSHTDLVRMKQGKIGIQFFSCFIECKDPDYLYEDFNKANSAVRDTLEQIDVTKRLVDEYSQHLSFVNTADEAMEVFQKQDGKIAITLGVEGLHQCDLSLAVVRQYFSLGVRYITLTHNCDNPFATAASSVDAGKKDNGLTKYGVDCIKEMNRLGMIVDLSHVSHQTMLDTLKVTKSPVIFSHSSVYSLTKHERNVRDDVLLKVKENGGVVCINFFPLFLRKEGESSVTIENAVEHIKYIIDLIGWDHVGFGSDFDGIPSGPSGLEDVSKYPDLVVKVWEATNATEEDIAKMMGLNVVRVWKECELVSKSLKSSKPFESNWEKRRWVFEEYCKEFPAMFPGAYEYKNNIYKSDQKLDNSTGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P018</accession>
    <name>A0A1E3P018_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DUF3955 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_69208</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC35F solute transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038003.1">
        <property type="nucleotide sequence ID" value="XM_019184159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P018"/>
    <dbReference type="STRING" id="683960.A0A1E3P018"/>
    <dbReference type="GeneID" id="30201405"/>
    <dbReference type="OrthoDB" id="1436450at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025016">
        <property type="entry name" value="DUF3955"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009262">
        <property type="entry name" value="SLC35_F1/F2/F6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051:SF0">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35, MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13127">
        <property type="entry name" value="DUF3955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06027">
        <property type="entry name" value="SLC35F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="484"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="520"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="554"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="589"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="618"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="644"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3955" evidence="10">
        <location>
            <begin position="331"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="129"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="215"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="672" mass="75717" checksum="B19482043BD4F445" modified="2017-01-18" version="1">MMGFFTKTHAVKKSVSSINSGDADLTSLDPPMLDIPMLHGNQPRRGSFASQLSQMKIITEQNSARFQTNAQKFTSPTDSEFSVKNMTLVSNGLKGEQERNMILEQQVRMLSHQAAMALDRLSDANKENQQLKDENETLKKRASHHRFSFGRRQSSYSSTSSTSSATNQSMFSNNKAVNYRSKNSSISSIQTNIDQLKSTNDSFFSEIHQNYGKEINTMRTELLSTQQELEQSKIEISQLKQQIQELLSKISQLQKENKSLLSKLSFKKQKQDVEKQFLTTQIDLLSTQLALKTDRAVLLASRINSYSNTDQIPVMTSLVNENRSHRRQRWTLGLIMLAVVVLSWVLSSFLISDIFENDLYRKPFFITYINTATFIFYLIPYLKKVIKGYIENGSLKYKEIEEDFLDSSDEELALNAESEYNAKLSLSETIKLSAQFCVVWYLANLATNASLSYTSVGSQTILSSTSSFFTLLIGALAKTESFSNVKIFGLVLSFIGVIVITKSDSDTVSSDIERTPLSIFIGNSLALAGALLYGVYATLLKYRIRDESRINMKVFFGFVGVFNLFFLWPSLIIFHYTGVEKFELPSGTFVISIILINCLITFISDFCWVKAMLLTSPLIVTVGLSTTIPFAMIGDIIFKGEKITVLYVIAASMICISFFVINKDAENDKHIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P021</accession>
    <name>A0A1E3P021_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription elongation factor Eaf N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_69039</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EAF family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037821.1">
        <property type="nucleotide sequence ID" value="XM_019184156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P021"/>
    <dbReference type="GeneID" id="30201402"/>
    <dbReference type="OrthoDB" id="3981290at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032783">
        <property type="term" value="C:super elongation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003711">
        <property type="term" value="F:transcription elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027093">
        <property type="entry name" value="EAF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019194">
        <property type="entry name" value="Tscrpt_elong_fac_Eaf_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15970">
        <property type="entry name" value="ELL-ASSOCIATED FACTOR EAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15970:SF2">
        <property type="entry name" value="ELL-ASSOCIATED FACTOR EAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09816">
        <property type="entry name" value="EAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription elongation factor Eaf N-terminal" evidence="9">
        <location>
            <begin position="13"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="134"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="416"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="152"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="223"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="240"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="257"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="302"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="342"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="426"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="437" mass="47742" checksum="72ED5D856768F130" modified="2017-01-18" version="1">MSHMELGIPDGEYELELSDVLQNQLGVSQDHNNGSSGPVRPIQRVNDEILSIRYGFRPDSIDDTQPAELTRDEKPSFQLKSQSTEHDQGIVFEGTSIQPKDLECILVFNPNSKSFELQKLDLQLRTNSIIKNRTLSNNNLKKPTKDQSPQPSETKSLSPSTTSSTALRKRPAKEPSIFQPKPRAKTPLSKPPVSGKSSTSKTSSETQPTGKSSDEDEVPLSKLTASKRSTVSKQSPKVNPSIKKPSSDSSTSPQKSTQNSSKISQKRTSPTPSVSPEKKSKPNVTKKSSTNSKLTEKKTISKSEPSTIHDTQNKAPTSRNTTPQPRSNSNNNTLKPLPNQVSSKKPPSTISTPTIQQESPSDDDEFGSLVDELEEELLSDDNFITIEDGSKDTSLSTSGDKTYDFGVKNGNGPINFRHLAGMSSKDEDDLSSSSEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P078</accession>
    <name>A0A1E3P078_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calcipressin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_63362</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RCAN family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038063.1">
        <property type="nucleotide sequence ID" value="XM_019183534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P078"/>
    <dbReference type="STRING" id="683960.A0A1E3P078"/>
    <dbReference type="GeneID" id="30200780"/>
    <dbReference type="OrthoDB" id="17212at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008597">
        <property type="term" value="F:calcium-dependent protein serine/threonine phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006931">
        <property type="entry name" value="Calcipressin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10300">
        <property type="entry name" value="CALCIPRESSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10300:SF14">
        <property type="entry name" value="PROTEIN SARAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04847">
        <property type="entry name" value="Calcipressin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="27608" checksum="F30B57E9726FF89B" modified="2017-01-18" version="1">MVELEDITNTLILAGIPLEKLEDNQFIVPLQHEIIANIPNTDLVQFIILKTFKRILIIFNTKQSSIKVYDHFKTTHDDFTTGYSLHDYSSFDINDNYLELPDNSRMFLISPPASPPIDFNYDQVEEEPNRLQIYTHEELQEIMKDNNTDFENLVTENLKKTKYSNNETSSSKRSIMDFAKEIILNDSNDDSKPKIILNPINGENDHLIGRAIQSFRTSLPPKSVFDELDDDIIDDGDYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P124</accession>
    <name>A0A1E3P124_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small ribosomal subunit protein uS2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RPS0</name>
        <name evidence="12" type="ORF">WICANDRAFT_32328</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. uS2 is required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with RPS21.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P124"/>
    <dbReference type="STRING" id="683960.A0A1E3P124"/>
    <dbReference type="OrthoDB" id="414863at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000461">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000010">
        <property type="entry name" value="40S ribosomal protein S0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03015">
        <property type="entry name" value="Ribosomal_S2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027498">
        <property type="entry name" value="Ribosomal_uS2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005707">
        <property type="entry name" value="Ribosomal_uS2_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01012">
        <property type="entry name" value="uS2_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 9 10" id="KW-0689">Ribosomal protein</keyword>
    <feature type="initiator methionine" description="Removed" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="208"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="231"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="modified residue" description="N-acetylserine" evidence="9">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ59028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="263" mass="29018" checksum="F5CEC6E6D76BD14D" modified="2017-01-18" version="1">MSLPASFDLTPEDAKLLLAANVHLGSKNVQVHNEPYVYKTRPDGVNVINIGKTWEKIVLAARVIAAIPNPADVVAVSSRTFGQRAVLKFAAHTGATAIAGRFTPGNFTNYITRSFKEPRLIIVTDPRTDAQAIKESSYVNIPVIALTDLDSPSEYVDVAIPTNNKGKHAIGLIWYLIAREVLRLRGSLPNRAEPWTIMPDLYFYRDPEEAEPTPEEAEEEQVEEQTEETQDWAAQPADADWAAETTEATADWTATEGTAQATW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P155</accession>
    <name>A0A1E3P155_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_84722</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038270.1">
        <property type="nucleotide sequence ID" value="XM_019185763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P155"/>
    <dbReference type="STRING" id="683960.A0A1E3P155"/>
    <dbReference type="GeneID" id="30203009"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140602">
        <property type="term" value="C:nucleolar peripheral inclusion body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140453">
        <property type="term" value="C:protein aggregate center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072380">
        <property type="term" value="C:TRC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:'de novo' protein folding"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:cellular response to heat"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:cellular response to starvation"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:cytoplasm protein quality control"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:protein aggregate center assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:protein targeting to mitochondrion"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:tRNA import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000048">
        <property type="entry name" value="DnaJ family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000001">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000036">
        <property type="entry name" value="Type I HSP40 co-chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="6"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="9">
        <location>
            <begin position="130"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="6">
        <location>
            <begin position="130"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="375"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="408" mass="44388" checksum="3EBB615AEAFAEF24" modified="2017-01-18" version="1">MVKETAFYDLLGVSPSASEAEIKKGYRKAALKYHPDKNPSEEAAEKFKEISAAYETLSDSQKRDIYDQYGAEGLASGGPGGMGGMGADDIFSQFFGGGMFGGAGGAPRGPQKGADIKHSISATLEELYKGRTAKLALNKTILCKSCEGRGGKAGAVKKCTSCGGRGVKFVTRQMGPMLQRFQTTCDTCSGSGDIIDPKDRCTSCHGKKTQSERKILQVHIDPGMKNGQRIVFKGEGDQEPDIIPGDVIFVVDEKPHELFKRKGDDLIYEAEIDLLTALSGGDLHIKHVSGEYLKVTIIPGEIISPGQVKVIENKGMPIYKLGGYGNMFVKFTIKFPENNFTSEENLKKLESILPPRRKENVPKDAVVDDVILTEYNEHKHESRRRPNYAHDDDSDDEGAGPGVQCASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1J6</accession>
    <name>A0A1E3P1J6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_32424</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038327.1">
        <property type="nucleotide sequence ID" value="XM_019181958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1J6"/>
    <dbReference type="STRING" id="683960.A0A1E3P1J6"/>
    <dbReference type="GeneID" id="30199204"/>
    <dbReference type="OrthoDB" id="2427554at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016287">
        <property type="term" value="F:glycerone-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07992">
        <property type="entry name" value="LPLAT_AAK14816-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052744">
        <property type="entry name" value="GPAT/DAPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31605">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31605:SF0">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="405"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="456"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="487"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="3">
        <location>
            <begin position="50"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="583"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="624"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="647"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="668" mass="75556" checksum="5ECD0E9BB46B6A58" modified="2017-01-18" version="1">MSSDQYEEPNFITKLGYDLMLWLLSSVFNCFFREIRPRGAFRIPKSGPCIFVAAPHANQFVDPVILMGQVKNETNKRVSFLIAEKSMKVVGVGHIARFAMPIGVKRPQDNLKTATGEISIDPSNPLKVIGHGTAFTKEATVKGLIGLPNSKGNAEIAEILDDSTLLIKKEFKKVAMDILKSPTKYKLADKVDQRQVYEKVFAHLSHGHSIGIFPEGGSHDRTDLLPLKAGVAIMALGAMQHNPQTDVKIIPCGMNYFHPHKFRSRAVVEFGHPITIPKDLVDRYNDPETSKAAVQELLDTITNGLKAVTVTCPDYETLMVVQAARRLYTNEKTLPIPLIVEMNRRLVIGYNHYKENPEIIELREKIMHYNENLKRLHLPDHEVNKAAQFNFLKNLSILIYRSLKLVLLLTLALPGIVLFTPVFIVGKMYSNKKKREALANSLVKIKANDVVATWKILIGIGFAPLLYIFYSCVGLYYFRDFKTINKFFLFWLIYISGATVTYSALIFGDTGMDILKSIRPIYLSLVTPQVLSELKIERKELAEKIVEIVNKFGPELFPDFDHLSKIQFDETFEDKKTEELRKRRRLKRKQERAKAKESLPSSDSDAESLSNAPIFANSSDAESEYSVTSTSETEGEQTINSTGISSKIRDTLRERRIHDENVNDVDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1J8</accession>
    <name>A0A1E3P1J8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Cyclin-dependent kinases regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_94595</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CKS family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038538.1">
        <property type="nucleotide sequence ID" value="XM_019186453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1J8"/>
    <dbReference type="STRING" id="683960.A0A1E3P1J8"/>
    <dbReference type="GeneID" id="30203699"/>
    <dbReference type="OrthoDB" id="440676at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061575">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.170.10:FF:000002">
        <property type="entry name" value="Cyclin-dependent kinases regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.170.10">
        <property type="entry name" value="Cyclin-dependent kinase, regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000789">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036858">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23415">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01111">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00296">
        <property type="entry name" value="CYCLINKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01084">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55637">
        <property type="entry name" value="Cell cycle regulatory proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00944">
        <property type="entry name" value="CKS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00945">
        <property type="entry name" value="CKS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 5" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00068939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU311113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="103" mass="12307" checksum="B474AF76EDC55334" modified="2017-01-18" version="1">MSFRGRQLTDSERVRVFEFQDSIHYSPRYSDDEYEYRHVMLPKAMLKVIPSDYFNSETGTLRILTEDEWRGLGITQSLGWEHYEIHAPEPHILLFKRPVNFGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1L7</accession>
    <name>A0A1E3P1L7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_32629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038440.1">
        <property type="nucleotide sequence ID" value="XM_019181987.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1L7"/>
    <dbReference type="STRING" id="683960.A0A1E3P1L7"/>
    <dbReference type="GeneID" id="30199233"/>
    <dbReference type="OrthoDB" id="566238at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01519">
        <property type="entry name" value="RHOD_HSP67B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE RDL2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086:SF10">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE_RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="92"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="202" mass="22615" checksum="E3AFCEC8F7371CB9" modified="2017-01-18" version="1">MLAATKKAVWKQVVVLPRIAAIPARSIFTSSSLYSKPSAITKSPIFLQAQRFQSSSAEKAAQVPEEVRKQLGQETPAKIYTYEEIKNLVTNPDPNKLLVDVREPSEVQEYAIPNSINIPYKSTPGALDLSPEEFEDVFKFEKPDKDKELIFYCVAGVRSTAAAELAQIFGYNHLGNYVGSLNDWKAHENIDLEHKDTISEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1Q9</accession>
    <name>A0A1E3P1Q9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_79863</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038552.1">
        <property type="nucleotide sequence ID" value="XM_019184965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1Q9"/>
    <dbReference type="STRING" id="683960.A0A1E3P1Q9"/>
    <dbReference type="GeneID" id="30202211"/>
    <dbReference type="OrthoDB" id="3163292at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="97"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="267"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="707" mass="80833" checksum="E0A19261653EA6C1" modified="2017-01-18" version="1">MAKKQIPIQPKSLNNNTGAGHRVTKLPTSASGQRSNSSTSSILSSSALNSPLSTTSSSSSSPIQNNAALLEPMISNSVFNLPLIPESTLPKPKILKSCIRCRKHKTKCDASEKTPNPCSSCAKKGIQCKIDYVLPPQRSDNLKNLVGQVETIKTNYELLEDYYTKISNRLNIKIESFEEWKLQNAQSTRDELIDNNNEEEDSEQKSSYNLIKLSDGSFISMNLVEDNQLLINNKLIQIDQFQEKLNKLSKRLTYLFQATLSQTKQQTDPSTPDNNSSTDDNSSIASPISAIDQEIINDVCQFDTGITLPQNNNIMQFLITPENHLATPPSSSVEILPDFKLFDLKFSVLDLFKDNKFLLILLVGFFYPDAYNSINSLNYNLFLSDYLNSYYQIMENQNKALNSSTYSSFLINSINPSYQLTFNKDQLKDLIDCEIFNSELYLKKIIQILFLNVLIYGLDEHFKILKDFIQFVKININSNRKKINFEKNVDLKFIDHYIKLFETFGESSEESTATTAVSSNSPIENFKQLLNFNLSFTNDYQLKKNQSLFNQTISNPSNDSLNSLKGCFKKFISNWSNYFQFYESELGLFIDDFIIEIFIINCLIFINEGHQDFNSILLDCLTSFKINNNESICELIEFFKTSKDELIQPNIEHFNFSCFQSQHIIMNLILNKKNSIDPKMNDIQMILENVDWKKETPDDVLKKIGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1S5</accession>
    <name>A0A1E3P1S5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_63369</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038069.1">
        <property type="nucleotide sequence ID" value="XM_019183535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1S5"/>
    <dbReference type="STRING" id="683960.A0A1E3P1S5"/>
    <dbReference type="GeneID" id="30200781"/>
    <dbReference type="OrthoDB" id="9986881at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140115">
        <property type="term" value="P:export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042908">
        <property type="term" value="P:xenobiotic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000082">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF7">
        <property type="entry name" value="DRUG_PROTON ANTIPORTER YHK8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="340"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="445"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="508"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="110"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="548" mass="61160" checksum="9484B4DC057CC49A" modified="2017-01-18" version="1">MSSSENSDHLKLEKSETHEEDKSITSTNNQHPYSFQPDQVDIDALSRQASRIASEQSRSCQDLQASSEDTVLNDNNNLSIEKDPNEVIFDGMDDKENINAKMSFFRKWSIVLVLCFATAMVTMLSSAWSYCEPHIIKEYHVGKEVGVLGISLIMFGLGIGPMLLSPLSEFYGRKPVFVGGLMLSCCFQFLTAFAPNLGGILFGRFMSGFFGSAFLSVVNGTFTDIFDKSQISLPIAFFGVAPFVGPSIGPLFSAGINKYLFWRWVFYIMLMLHGVALAFVILFVPETYVPVLLVKKARRLRNQIGNDKLYAALEKDQRSVIMTLLKAPNRPFLLLLRDPMIAVLCFYSGLVLAIIYLFFVAFPYVFQTVFGFDEVSQGLSFIGLLVGLCFGAATAPSFQNYYNKIVEKNGVAYPEDRLYSLMIASIILPISMFIFAWTSYPQVHWIGPIIATAIYGYGCSLAFTGIIGYTADGYKLYSASAMACNSLVRSCMAGGFPLFGLQMYKKMGVNWASCFLAFLFVLMIPFPIIFYKYGKVLRSKSPYAWSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1X2</accession>
    <name>A0A1E3P1X2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_79861</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038550.1">
        <property type="nucleotide sequence ID" value="XM_019184963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1X2"/>
    <dbReference type="GeneID" id="30202209"/>
    <dbReference type="OrthoDB" id="10627126at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="184" mass="20119" checksum="7D97541738CFA33C" modified="2017-01-18" version="1">MFLIKYKPRIYIKDIQSLVQIVIMVAIKNTIALASVAALSSAAAIPDAHFGHGWGGDPGWGEGPRPTKSWGHFPPTNGSFPTFSHHPKPTGWGHHPFPTHQWPTAWPTEWSFSWPTAFPTDWPTEWSAAFPTGWPTSCPDYFPDFKNLESAPSQPTISAAPTVYSAPTEPASGSPSGESQFIYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P247</accession>
    <name>A0A1E3P247_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_31518</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038179.1">
        <property type="nucleotide sequence ID" value="XM_019181844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P247"/>
    <dbReference type="STRING" id="683960.A0A1E3P247"/>
    <dbReference type="GeneID" id="30199090"/>
    <dbReference type="OrthoDB" id="2098326at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF9">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="87"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="28466" checksum="57CBA542B8366227" modified="2017-01-18" version="1" fragment="single">LEKSRAHRILWLLEELELEYELKLYHRDPATGLAPKELSDIHPLGKSPLLELDYRDGKETKKIAESGLIITYLIRHFDTDGKLKPINEEDEEEAEYYLHFSEGTLQPLLTYTLLHEIAYSRTRESAKPAIDTFLDRVDGSYTTPNLSKAFDSLEQKLKKRCEELEYTSSSKEIFFVGHKLSAADIILEFNASLAFRDPELIKPIHRSKYVYLSKWLSQIKQRPAFVRSVDKIQKMGNDEYTIYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P298</accession>
    <name>A0A1E3P298_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SVP1-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_32070</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038229.1">
        <property type="nucleotide sequence ID" value="XM_019181916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P298"/>
    <dbReference type="STRING" id="683960.A0A1E3P298"/>
    <dbReference type="GeneID" id="30199162"/>
    <dbReference type="OrthoDB" id="1667587at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="42656" checksum="339FA4F9E7FA98C1" modified="2017-01-18" version="1">MNIRKAINEPDTTGSSKLLCASFNQDQSCFAVGYENGFRVYNTDPMDLKVKRELGSNGGIGLIRMLYRTNYLALIGGGKNPRFPLNKAIIWDDLKTKDALNLNFYSPVLNIFLSRTRIVVVLNNKIYVHGFNSPPKNIAQYETFDNPLGIAALSPGAYQDQASNLQILAFPARVMGQIQIVDISSSGQERNLVSIIKAHKSKIRCLALNKAGTMLASASETGTIIRIHSTQTCSLLYEFRRGLDRAIIYSMEFSQNGTKLAVLSDKQTLHVFNITNNQQQVNKHHLLKNLPGLLRPNYFNSTWSFCSVHLKDEETDSDLDDRGVLGWSSESSIIILWKMKGKWEKYVIVEKDEVNSGGESFKKFELVREGWRSFGDLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2B3</accession>
    <name>A0A1E3P2B3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_79990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038690.1">
        <property type="nucleotide sequence ID" value="XM_019184984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2B3"/>
    <dbReference type="STRING" id="683960.A0A1E3P2B3"/>
    <dbReference type="GeneID" id="30202230"/>
    <dbReference type="OrthoDB" id="413993at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053044">
        <property type="entry name" value="Metallo-hydrolase/TatD-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47345">
        <property type="entry name" value="CUT9-INTERACTING PROTEIN SCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47345:SF1">
        <property type="entry name" value="CUT9-INTERACTING PROTEIN SCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR005902-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="319" mass="36678" checksum="E8C908388E7B39EC" modified="2017-01-18" version="1">MLIDAHCHVSTECDPLRIDELVKEYAQFEDCLKLILMSTNHIDYQYVDKLAQGLSVMVTPCFGVHPWYSHLFSLDGIIGKEEHYRQVFGIKEESNLDDEILDILPDPIDFNQHLQILRNLINKYDIVVLGEIGLDKLFRIPTSGFYGNPKFKESKDVVGLTNYKTTMEHQKRIFIEQLKIAVELNLPISVHNVKTSGVIHDIISKELLKSDYSSSVCLHSYTGSIETLELFLKSFKKSKASFYVSLSNFINNSEAKKQELEGILRVMPRGNVLTETDMTIDNMNPIEQLKSIRGSVLRRDDEDDDSLIEGNYHRYLDNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2E6</accession>
    <name>A0A1E3P2E6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ERCC1-like central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_19970</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERCC1/RAD10/SWI10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038705.1">
        <property type="nucleotide sequence ID" value="XM_019180848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2E6"/>
    <dbReference type="STRING" id="683960.A0A1E3P2E6"/>
    <dbReference type="GeneID" id="30198094"/>
    <dbReference type="OrthoDB" id="10262814at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905348">
        <property type="term" value="C:endonuclease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070522">
        <property type="term" value="C:ERCC4-ERCC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006277">
        <property type="term" value="P:DNA amplification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000736">
        <property type="term" value="P:double-strand break repair via single-strand annealing, removal of nonhomologous ends"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000710">
        <property type="term" value="P:meiotic mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000715">
        <property type="term" value="P:nucleotide-excision repair, DNA damage recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22325">
        <property type="entry name" value="ERCC1_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000015">
        <property type="entry name" value="SsDNA endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047260">
        <property type="entry name" value="ERCC1-like_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004579">
        <property type="entry name" value="ERCC1/RAD10/SWI10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00597">
        <property type="entry name" value="rad10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12749:SF0">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12749">
        <property type="entry name" value="EXCISION REPAIR CROSS-COMPLEMENTING 1 ERCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03834">
        <property type="entry name" value="Rad10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC1-like central" evidence="8">
        <location>
            <begin position="86"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="59"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="197" mass="22331" checksum="6C1B7F456B3AFED7" modified="2017-01-18" version="1" fragment="single">QSNTDTTSFASILAGVQKLRGEPSSNDSNSAPIAQQSPVTRQPQAQVQQPAPSSKKRTNAFNQDRASYNSPKTQPFQKSGSPKPSTISVNKTQTGNPLLQHIINVNWTYVESRNTYDYLVKNRQVIFLSLKYHKLHPEYIQNKMKPLLKKNALLLTVVDVENSESILREINRICLFNEFTLLLAFNFEQAAKYLTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2H6</accession>
    <name>A0A1E3P2H6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_30760</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038735.1">
        <property type="nucleotide sequence ID" value="XM_019181757.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P2H6"/>
    <dbReference type="GeneID" id="30199003"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18597">
        <property type="entry name" value="ABC_6TM_YOR1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18606">
        <property type="entry name" value="ABC_6TM_YOR1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000010">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000997">
        <property type="entry name" value="Multidrug resistance-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="356"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="389"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="747"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="785"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="872"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="969"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="135"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="456"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="753"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="1065"/>
            <end position="1305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ59528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1313" mass="146698" checksum="11C0F1193AF8DA08" modified="2017-01-18" version="1">MTRDLDRAHEDILVDEKTVKPVKRFLTPILTKKVPLVPDESERKTLPLYSSNILSRIFFVWLIPLFNRGYKRTLDFNDLWKLDDRLSIHTTYPKFQHHLNEIITKYRLKTGEKNKFPKFAILQALFLTFKYECGLAVGLSMLHYGAQVVSPILIKKLINVVEMRVLVPSSPINDGIGYAIGVTLLVAISALAQNHSLQNSKLVGSHARTILTKALLEKSLNADSKTKHNFGKGKIFTLMSSDLAKIDLGLSYLPGALSFPIPSVVAIVLLIINLGVAALSGIGVFVLTVSLFVFPGILFMKYKEKSSLHTDKRVSLMREIINAMKMIKFHAWEGSYEKKVIEERSKESSYIFKCEVLINIMFGAVFNLSLTSSMTAFLVLYAIRFNHGNVGSIFASLALYNIFTMAVSEFPMIIGQCTSGWVSLNRVREFLESSIENSDTNLSEKSSINYKSDSAINITNGEFEWEAFNDDDDDFKLKNINLNIQQGEFIVITGPTGSGKSSLLQAINGEGINKLQGSVSINGSSIFYEMPWIQSATVRDNITFGKHFDAILYRKVIKACALESDIALLQAGDLTEVGERGVTLSGGQKARISLARAIYADKDVYLLDDVLSAVDSKVGAHIVDHCFMDLIASKTKVLVTHQLSLIYKADRVVFLNHDGSIDVGTVNQLMNSNPKFVTLMQHSQIKSTKLKKSTSKVITNSIDAEDYDNFANGESGSLYGLEEKAVNAIPLKIYNQFISAGGGRYPYLTITCLVLITSLTVFCTMFANVWLSFWIDQKFSKPGPFYIGIYIMLTFCSLIFVIFELIILGSISVNAGKNLNLQAVKRLLHTPMSFIDTTPSGRILNRFTKDTNSLDNEIGNQLKKFIHISATIIGILILCVVYLPWFAIAIPLLFLIFFTVTNYYQASSREVKRLEALSRSLVCNNFNEVLNGIDTIKSFGSEHRFISKNDKLVDRLNEAYLVTVSNQRWIGISLDLIGTGLVFIVVMLALTRQFNISAASTGLITTYVLELGNEISDALVSFSEVENEMNSVERVCHYANELDQEAAYKVVGLVHAPEWPQNGVVELKNVSLRYREGLPLVLKSLNFKVGSNEKIGIVGRTGSGKSTIVNALFRILEIAGGKIEIDGLDIKKLGLYDLRSKLSIIPQDPVLFEGSIRKNLDPFNERSDMELLDALRRSGLIADGELKNIQRNHKFHLESVVEDEGSNFSLGERQLLALARALVRRSRILIMDEATSSVDYETDALVQKTISQEFKDCTVLCIAHRLKTILKHDRVLVLEKGELVEFDEPKALFNRNGRFREMCDSSDITLSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2J7</accession>
    <name>A0A1E3P2J7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Proteasome alpha-type subunits domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_31657</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038334.1">
        <property type="nucleotide sequence ID" value="XM_019181864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2J7"/>
    <dbReference type="STRING" id="683960.A0A1E3P2J7"/>
    <dbReference type="GeneID" id="30199110"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03754">
        <property type="entry name" value="proteasome_alpha_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000026">
        <property type="entry name" value="Proteasome subunit alpha type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034642">
        <property type="entry name" value="Proteasome_subunit_alpha6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="8">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="244" mass="27071" checksum="6DF6ABC821817715" modified="2017-01-18" version="1">MSGAAGFDRHITIFSPEGRLYQVEYAFKAINSSNLNAMGVTGKDSVVIISQKKVPDKLLDPKTVSYIFPITPKIGLVATGSIADARSQALRARAEAAEFHYKNGYQMPVDALSKRMANLSQIYTQRAYMRPLGVALTFCSVDDEFGPLLYKTDPAGYYVGSKANATGPKQQELTTALEKKYKKKDHVEGDWKQVVEFAIITLSNTLNTEFRKNDIEIGVATPEKFFTLTPDEIDERLVSIAEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2L9</accession>
    <name>A0A1E3P2L9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_84783</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038349.1">
        <property type="nucleotide sequence ID" value="XM_019185778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2L9"/>
    <dbReference type="GeneID" id="30203024"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="59" mass="6408" checksum="81D848E3AEFA5F4A" modified="2017-01-18" version="1">MACEISDLGAGKGDFCMTRVVLIGKMCFRVFGLVGNLFFTLILAHLQGVSRLFPSLAEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P314</accession>
    <name>A0A1E3P314_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_33228</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038484.1">
        <property type="nucleotide sequence ID" value="XM_019182046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P314"/>
    <dbReference type="STRING" id="683960.A0A1E3P314"/>
    <dbReference type="GeneID" id="30199292"/>
    <dbReference type="OrthoDB" id="27218at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071528">
        <property type="term" value="P:tRNA re-export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000490">
        <property type="entry name" value="CRM1p Major karyopherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041123">
        <property type="entry name" value="CRM1_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041235">
        <property type="entry name" value="Exp1_repeat_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014877">
        <property type="entry name" value="XPO1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040485">
        <property type="entry name" value="XPO1_repeat_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF2">
        <property type="entry name" value="EXPORTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08767">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18777">
        <property type="entry name" value="CRM1_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18784">
        <property type="entry name" value="CRM1_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18787">
        <property type="entry name" value="CRM1_repeat_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01102">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="6">
        <location>
            <begin position="34"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1081" mass="122720" checksum="29B4A4ED9FCF2F33" modified="2017-01-18" version="1">MDSILDFTKDLDIQLLDKVVETFYAGSGPDQKVAQDVITKFQEHPDAWQKADQILQFSSNSQTKYIGLSILDKLIQTKWKMLPDEQRKGIRNFIVNMIIALCDDDDQFQSQRSLINKCDLTLVQILKQEWPENWPTFIPEIVQSSRAGFNVCENNMVILKLLSEEVFDYSAEQMTQAKIKNLKTRMGEEFSEIFKLCYEVLDKATRPSLIVTTLKALLKYLQWVPLGYIFETDLLGLLTNKFLGPADTRAITLKCLTEVASLTSPNYDAKFVEMFKNSISQIANVVPPGSDLRRSYQVANSNDQAFLQDLAMFICTFLANHVLALEANEADRELLTTAHEYLIQLSKIDERELFKTCLDYWGKLVSSLFAEIQQLPANELSPLMQLGYSQALSSSSAGGAPNPELLNKYPLRKHIYNNILSNLRLVIIESMAKPEEVLVVQNDEGEIVREFVKESDTITLYKTMREVLVYLTHLDVVDTEQIMNEKLAKQIDGSEWSWHNINTLCWAIGSISGTMNEEMEKRFLVSVIKDLLALTEMKRGKDNKAVVASNIMYIVGQYPRFLKAHWKFLKTVINKLFEFMHETHEGVQDMACDTFIKITLKCRRHFVVQQQYETEPFIDEIIRNIQSITEDLQPQQVHTFYEACGYIIGAQTSVPVRDRLLGDLMHLPNLAWTTIVEKAGQDPTLLTNPETVKIIANIIKTNVAVCSSLGPGFYAQLGLIYENMLSLYKAVSSMISDSVAADGLIATKTPKVRGLRTIKKEILHLIEAYISKGENLEQIVGTLVEPLLAIILEDYKSNVPDARDAEVLNCLTTVVSKVGHMIPEGVVLILQNVFEPTLGMITNDFTEYPEHRVEFYKLLREIDLKAFNALLQLSGDAFQLLINAILWAFKHNNREVESSGLSLAIELLKNVEKLGTTPFTTAFYQNFYFPILADTFYVITDSDHKAGFKSQSQLLSKLIELVEGGHIKEPLYSESQAPAGTSNAVFLHEYLANMLISAFPQLQQDQVENFIKALIAQHRDQVKFKGTLRDFLVQIKQYGGDATDYLFAEDREYELAEKQRLEREKASKVGGLLKPSELDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P371</accession>
    <name>A0A1E3P371_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinetochore protein Spc24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_78337</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
            <location evidence="10">Centromere</location>
            <location evidence="10">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the SPC24 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038907.1">
        <property type="nucleotide sequence ID" value="XM_019184707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P371"/>
    <dbReference type="STRING" id="683960.A0A1E3P371"/>
    <dbReference type="GeneID" id="30201953"/>
    <dbReference type="OrthoDB" id="3344830at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:Ndc80 complex"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ndc80_Spc24"/>
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        <property type="entry name" value="Spc24_Fungi_globular_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22142:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN SPC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08286">
        <property type="entry name" value="Spc24"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Kinetochore globular domain"/>
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    <keyword evidence="8 10" id="KW-0131">Cell cycle</keyword>
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    <keyword evidence="4 10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
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    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="20"/>
            <end position="123"/>
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        </source>
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        </source>
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            <dbReference type="RuleBase" id="RU368011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ59700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="193" mass="22716" checksum="F427DA2B1E648F33" modified="2017-01-18" version="1">MMLQLDPAELLRETLEGCDIETDLDTIDRIAENLKQLQDERNQNIITEQQELQELSEKLADEQQNIESLENSSTRQEIKSKLKTNQSLELSMNKNLKELTSIKQDLSTNLSNLVDDYNSLDKQIEELDFIEDDEDKDAIVLKLMVYRKFGLKIDIPSNTLIIYNKEKNLTDFLNYGDEKYSDYFISNYIWDRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P389</accession>
    <name>A0A1E3P389_WICAA</name>
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        <recommendedName>
            <fullName evidence="5">DOA4-independent degradation protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_83797</name>
    </gene>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
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    </dbReference>
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        <property type="project" value="EnsemblFungi"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
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        <location>
            <begin position="179"/>
            <end position="227"/>
        </location>
    </feature>
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        <location>
            <begin position="15"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ59720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="26024" checksum="39ECDF824B3643A5" modified="2017-01-18" version="1">MQLFEWAFGKKLTPQERLRKNQRALEKTQRELEREKNKLEQQQKKLIAEIKKSAKNGQMGACKIQAKDLVRTKRYIEKFNSMKTSLQAISLRIQAVRSSDQMTQSMKDATRLLGSMNRSMNLPQLQKIAMDFEKENDIMDQRQEMMDDAIDDAIGDEYEDDEEADEIVNKVLDEIGVDLNSQMKDTPQGLDTGVAERERVPQAVGANGEGDGEDDDLQARLNSLKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3A4</accession>
    <name>A0A1E3P3A4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_16864</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019180713.1"/>
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    <dbReference type="STRING" id="683960.A0A1E3P3A4"/>
    <dbReference type="GeneID" id="30197959"/>
    <dbReference type="OrthoDB" id="10070851at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd13280">
        <property type="entry name" value="PH_SIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="BAR_dom"/>
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        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Sip3_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14248">
        <property type="entry name" value="CYCLIN Y, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF16016">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="952"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="983"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="278"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="8">
        <location>
            <begin position="727"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="683"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="683"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1123" mass="128476" checksum="983A1CFE8D9EA99D" modified="2017-01-18" version="1" fragment="single">IKLISIKLKEAALDSPTFRTGVNYFNSQIDHTEQWIEGLLKSLRKYPQQFNDFKEVNNVLFNQLIPDFLKDGLINQDNSSSILDTSKTNLSSLWEGNLKLLDVKETEIVQLLTDLQRTHLKHYREIRKNFEFFQNKFDSLLSKYSSQSKTKEPSALREDAFQLFEVRKSYLNASLEICVEISKLHQSLDSCLVELAELLFKDKDSFDEKYFEKVKAWSGAVADSVSTLLGDMIQSKNQIEDATISQFAPSRDLIQHNPALINPNTLTDPDPENPDDKSYEKHGWLFMKTTVGKPARQIWVRRWVFVKSGIFGLFSIAPSKNFVQETDKIGVLLSNIRYAPDEDRRFCFEIKTIDATIIFQAENLKELKSWLRVFSLEKQRAIENSDSEAGEYAFGRYPPLLYEFASTSLTSVDLELTTSRAENTRNDDSQTTVVSSYLSQLVGGNENLDFRYSGSAGEFQEPDINTPITTKMSKCAILAHAFLDSTVIPTAVTANIWGSVNWGMYYVSDSSVQPNNKAKAIQSRENNLGSLSKAERYPHYYPQHLKNYDVQLKALFDTGIPKEELVVTHFSCLWSLNPKQELSGRCFVTTQNCYFYLNSTGFVALLKKPLVELVAVDVTVEKDWDTLKVYDIEGLNMKGRIFLDDGRLIQKKIDLLINNLAKDKPKPQSEIIPLLESIESEELQKSERKQSLRRKDGADITPPLNSELQSFQPFSGVVSHNKSMKTNYYNEFDQSTVKTLDAPAKAVFHMLFGDHSSILREAITFLIHDEFITTPWYQNGSELTRNITFKMRLSKKLVSIRDSDGSVTSGYIQSIEDFVDGKYYRIREQRIPVKLASGDSIQLIKKYVIIEVDARTSKLFIFSKVAHTQSSYKSFLASFIKSSTKRLVKDEDKLIIDSVANSIQRLGSHGKVIKAIRTYGNLSKAEGKYTEPNTSMFEFTFNMMLNFYLKRFFYVFAAGILEFSKLFFSGCFRLIKALSMNKLILLLLGISVFFNIFLSGRSTVSFWTAKRAEHLVKDFAHELSINKMERSIYLNDLDALSGFNFNESSECFDKFMSSKIRDDSGSQTDSRFREARHQLAVKRNELLVELKILEKVEKELVAGSWKNFLLSELHLCKVSLDDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3B1</accession>
    <name>A0A1E3P3B1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-dehydropantoate 2-reductase</fullName>
            <ecNumber evidence="4">1.1.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ketopantoate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_78377</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16233"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.169"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.169" evidence="4"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038947.1">
        <property type="nucleotide sequence ID" value="XM_019184713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3B1"/>
    <dbReference type="STRING" id="683960.A0A1E3P3B1"/>
    <dbReference type="GeneID" id="30201959"/>
    <dbReference type="OrthoDB" id="3609at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008677">
        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000017">
        <property type="entry name" value="2-dehydropantoate 2-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003710">
        <property type="entry name" value="ApbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051402">
        <property type="entry name" value="KPR-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00745">
        <property type="entry name" value="apbA_panE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708:SF30">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708">
        <property type="entry name" value="PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0521">NADP</keyword>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase N-terminal" evidence="5">
        <location>
            <begin position="8"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="6">
        <location>
            <begin position="197"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="340" mass="37837" checksum="8ED586D4BFC05775" modified="2017-01-18" version="1">MSSPRSKVLIVGLGGVGVLAAYALEYNKKAEVTALIRSDYDRVIEKGYEINSVDYGHIEGFRPTHVVKTIEDAKKYGEFDFILVTTKNIPDIHVVEDAFADAVSDKTTIVLLQNGFGIEKATFERFPGHIVLSGVSMISSANFHGVVEHVGTDSLGIGYFDNGVSSKEEQQAKTKQFIELYENDAIDCHIDEDVKFSRWRKLVYNASFNTIGALVDLDVGRIQEAGGTDTLIKPTMREIFRVAASDGVTLDESLMDTMIHSDDGNWYAPSMLVDVRKGNQIELEIILGNVLEIAKQNNVEVPILSVLYNLLHLVQFRLKEQNGLINLPEERPLKKQKTSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3G1</accession>
    <name>A0A1E3P3G1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein-sorting-associated protein 24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_63911</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038624.1">
        <property type="nucleotide sequence ID" value="XM_019183636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3G1"/>
    <dbReference type="STRING" id="683960.A0A1E3P3G1"/>
    <dbReference type="GeneID" id="30200882"/>
    <dbReference type="OrthoDB" id="2329734at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904669">
        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070676">
        <property type="term" value="P:intralumenal vesicle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="26013" checksum="271143E1EDE71CEA" modified="2017-01-18" version="1">MNYLKKAIWGPDPKEQHRKCKQLVRRNGRQLEKSIRELGSLEKKTESLIKGAARKEDYKTVKIYARELYGIKKQKSRLHKSKAQLDSVGMQIDESFGMLKLQENMKLSSDIMKEVNNLVKLPEITGTMRELSQELVKSGIMNEMVGDTVDMLDENEDEFEDEEVDEEVNKIVSEFTKDKFNKVENAPDSKLEVPIQPVEEEEPVEEDDEHVLNEMRERLKALQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3G5</accession>
    <name>A0A1E3P3G5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_92024</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039162.1">
        <property type="nucleotide sequence ID" value="XM_019186334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3G5"/>
    <dbReference type="STRING" id="683960.A0A1E3P3G5"/>
    <dbReference type="GeneID" id="30203580"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="673"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="705"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="744"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="779"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="809"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="904"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="333"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="958" mass="107474" checksum="EE685AAF698B25BC" modified="2017-01-18" version="1" fragment="single">VIQALIAGSLYYGTPATTRGSFSRSGSLYFMILYFSLMGLAEVSGQFADRPILLKQKSYSFYHPSAETISATLTKFPFKLLSVTAFYLITYFLSNLTRDAGKFFLTYLFLVVSSETITAMFQMVAALSQNVAGANAVAGILMLAISLYSCYMIQLKSMHPWFKWISYINPIRYGFENMLADEFHGRKMDCGGSLVPSGPGYENVDSANQVCAFVGSVPGQSWVSGDSYLKVQFNFKYTHIWRNFGILIGFFIFFLGVNAICTEFKRPVKGGGDHLYFKRGGKPSDEVLLSSDAQAADIEAGTSDRPIQDHDLKSGSSSTDNDVFEGLGSKSVFAWQNVDYVIPYKGGSRKLLDNVQGYVKPGTLTALMGESGAGKTTLLNTLAQRIDMGTITGSMLVNGKPLDTSFKRSTGYVQQQDLHIAEMTVRESLQFAARLRRPKSVSDSEKLDYVEKIIKILQMEAYSEAIVGTLGSGLNVEQRKKLSIGVELVAKPSLLLFLDEPTSGLDSQSAWAIVHLLRQLAQAGQSILCTIHQPSATLFEAFDRLLLLKKGGQTVYFGNIGENSRILLDYFERNGARKCEKHENPAEYILESIGAGATASVHEDWYEKWANSQEYLDTTREIEILMRETNEKLGDQDQASNKELQSTFALPYLDQLYYVTRRTGIQFYRDPQYIGAKFSLMITGGLFVGFTFWSLKETIIGMQNGMFVVFMAIIISAPAINQIQERAVASRELFEVRESKSNTFHWSTLAAVWYLNYSIIYQFYYISLGLFIVYMSPDLASSTVITGLFLTMMISFCGVVQPMSLMPGFWTFMWKVSPLTYVIQTLLSLILHERKIRCGKDEYNYFEPPSGMTCQDFAGPFVNHVSGYLQNPSATSNCAYCQYSVGDDYLKTVSVSFGNRWRNFGFMWVYIIFNIFTMCAVYYIFRVANISPLGFIKEKLEASKRKREEKKGQNVSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3P6</accession>
    <name>A0A1E3P3P6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_78171</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038729.1">
        <property type="nucleotide sequence ID" value="XM_019184676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3P6"/>
    <dbReference type="STRING" id="683960.A0A1E3P3P6"/>
    <dbReference type="GeneID" id="30201922"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045290">
        <property type="term" value="F:D-arabinose 1-dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052588">
        <property type="term" value="F:diacetyl reductase ((S)-acetoin forming) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045149">
        <property type="term" value="P:acetoin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="23"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="4">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="324" mass="36592" checksum="4EA3F522F6126256" modified="2017-01-18" version="1">MPTHQLAIDHKVKLNDGYSIPAIGLGSATPKDDHSRLEEAVKSAIRAGIRHIDTAWYYGSEPAIGKALKELFDNGEVKREDLFITTKVWPALWDRAEKSLNESLKSLGVDYVDLLLQHWPVCYDTDENGEPNNPYDENGVLHFAKNGDYVETFKQIVKLRDTTDKVRSVGVSNYTEKLLQRAIDETGVAPVLNQIELHPRIPQKKLVKFFESKGVKIEAYSPFGAQGAPLLEEPKVKALAEKYTVSPNVILTSYHILSGRIVVARSSNKDRVKDFINLAELTKEEIEELDQIGIENPQRFINEGWGIGLGFEHWKTTPSWEEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4V2</accession>
    <name>A0A1E3P4V2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phytanoyl-CoA dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_54120</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PhyH family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039517.1">
        <property type="nucleotide sequence ID" value="XM_019182733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4V2"/>
    <dbReference type="STRING" id="683960.A0A1E3P4V2"/>
    <dbReference type="GeneID" id="30199979"/>
    <dbReference type="OrthoDB" id="445007at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008775">
        <property type="entry name" value="Phytyl_CoA_dOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20883">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20883:SF15">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05721">
        <property type="entry name" value="PhyH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="33041" checksum="9D0BC388053B6316" modified="2017-01-18" version="1">MFTKEQADQFDKEGCICIPDYLSEEDISKLLNYTHKLIDEFDASSHPLTKFTTGDNDNEGHVGDDYFLNSSDKISFFMEPGAFNERNELTKPKDKAINKIGHGLHLDPVFYPYTINPTTKEIVDKLGFQDPRVLQSMIICKQPEIGGEVPSHQDGTFLFTKPQSAIGFWIALEDCDENNGCLSYLPGSHKLVPIQKRFVKKEGGGTGFIYFNEEQRDEYDNDEKDYVKIKCRKGSLILIHNSVLHRSNLNFSDSSRYAYAFHVIDGAAEYDELNWLQVPPSGGANFTKLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4W6</accession>
    <name>A0A1E3P4W6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_84205</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039527.1">
        <property type="nucleotide sequence ID" value="XM_019185664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4W6"/>
    <dbReference type="STRING" id="683960.A0A1E3P4W6"/>
    <dbReference type="GeneID" id="30202910"/>
    <dbReference type="OrthoDB" id="421009at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090083">
        <property type="term" value="P:regulation of inclusion body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd15844">
        <property type="entry name" value="SNARE_syntaxin5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021538">
        <property type="entry name" value="Syntaxin-5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF3">
        <property type="entry name" value="SYNTAXIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11416">
        <property type="entry name" value="Syntaxin-5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="9">
        <location>
            <begin position="219"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ60320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="310" mass="35847" checksum="821675FD687D9201" modified="2017-01-18" version="1">MELETAQDIRNRTVEFQQCVTTFNKRINKSQHQNNQQVAPPNIKRSEFQQRASKIAKDISHTSELLGKLALLAKKKPLFDDKPIEISELTYVIKQDIVKIEKNLKNLQDYLKTGNETSNEELKTNSKNIVQLLNTKMKNVSGNFKEVLETRQKNELENKSRKEKFFSTLQQQQQLTNHATTVASDNPFLMDTPSNGGENNALLSLPQDQQLQLLEEQSSQYMQDRNLAVETIESTINEVGNLFQQLATMVNEQSEVIQRIDTNVEDIDLNITGAQRELLKYFNSINQNRWLYLKIFGVLIIFFILWILVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5D8</accession>
    <name>A0A1E3P5D8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_23284</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039896.1">
        <property type="nucleotide sequence ID" value="XM_019180983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5D8"/>
    <dbReference type="STRING" id="683960.A0A1E3P5D8"/>
    <dbReference type="GeneID" id="30198229"/>
    <dbReference type="OrthoDB" id="2573941at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070274">
        <property type="term" value="C:RES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000384">
        <property type="term" value="F:first spliceosomal transesterification activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000349">
        <property type="term" value="P:generation of catalytic spliceosome for first transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051237">
        <property type="term" value="P:maintenance of RNA location"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12411">
        <property type="entry name" value="RRM_ist3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051847">
        <property type="entry name" value="RNA_proc/Spliceosome_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045844">
        <property type="entry name" value="RRM_Ist3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45880">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN, X-LINKED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45880:SF1">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN, X-LINKED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="32"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="107" mass="12271" checksum="83229D1B2EE8A1FE" modified="2017-01-18" version="1" fragment="single">SQVRNIERLNQRELEAGISIEASWHNEFKDSAYIFFGSLPYELTEGDILIIFSQYGIPVHLKLVRDKETGKSKGFGYLKYEDQRSTILAVDNLNGTKVLGRIIRVDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5M5</accession>
    <name>A0A1E3P5M5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">12 kDa heat shock protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_18578</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039751.1">
        <property type="nucleotide sequence ID" value="XM_019180794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5M5"/>
    <dbReference type="STRING" id="683960.A0A1E3P5M5"/>
    <dbReference type="GeneID" id="30198040"/>
    <dbReference type="OrthoDB" id="2348401at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007250">
        <property type="entry name" value="HSP9_HSP12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04119">
        <property type="entry name" value="HSP9_HSP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002590">
        <property type="entry name" value="HSP9/HSP12_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="11388" checksum="F7637345F6AB1473" modified="2017-01-18" version="1" fragment="single">MSDLGRKDFTDKVQEKVTPQDQKSYIDQAKEGITDAADKASRNLQPEGAKSDTQKLGDSVQKGHDDAKSANAKPLSETAGEYVESAKQGLNQAAEYISNTLTGAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5S4</accession>
    <name>A0A1E3P5S4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cohesin loading factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_77274</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SCC4/mau-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039801.1">
        <property type="nucleotide sequence ID" value="XM_019184529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5S4"/>
    <dbReference type="STRING" id="683960.A0A1E3P5S4"/>
    <dbReference type="GeneID" id="30201775"/>
    <dbReference type="OrthoDB" id="3981211at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019440">
        <property type="entry name" value="MAU2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10345">
        <property type="entry name" value="Cohesin_load"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="62"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="102"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="151"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="73"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="161"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="255"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="274"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="970" mass="110834" checksum="AB9B556AA0AC2A4A" modified="2017-01-18" version="1">MDRGYYQGQGYPNYNNSQWQNQQQQQQQNNAQFNNAQYQQPQYPFNYNQQNNVQYQGQENAFPHYNQQPIPPYQVNYPQQQPQQQHQFHQPVPQNQYYNQYQEPLNPYPQQTPYQFDSSSSQPSVPTQQPYRLQQPLSQPVLPPNYHQYQQQYSNTSSPAPQFAPTFQQSSPSSAPQPIPYTEPEVIAIPSPSPPYVAPVPAPVAQQRAPNIKPQVPQHVNTPPKPPQNQTASKVQAPVYSQPKHHQLPPPKVDQTPRQEKRKAEEQLTPLKATVNSSQQPTPSFNAAPMEPKLLSSRGFSFDYSNAKDNKSSPAQTKKSLKDSSQTKAPSQAELVSLLNNDKVRSQQLLDLSDQFYSHAVSQMKNIKTKKNLQEFYTLIQLSISCLEKILEMKNKVSLALRIKCHNKLGFIKNNNCNIDQDSSTLEHHYDQVIMLCGNNFEFKREKCLAQAGIIISLIDTKKYRQALKFVNETIEEWGLENASLLVYFKLQILLLSEPSQALNFLQNYISDNKTTSGITSFFKIIEICLSLYRGMKPMSTLESIDNTDWADSKETSDILLLFTKILVQINHRDLDSSEKLESLFKTAAGSINALDPEKTFTVNFQIPGGDILPIGLEPITCENLNILTCLLYGITLLNSTYEKRKPLKLFDRALGMIDSALFQINDHQKSTTSISNRVLKLNYLKNLTIFYKLMESFIMEPYSSTLVKEYEKSTASIPKYLQESGQLRTLFVLGLQAQLNSDLSRAKNYYNELLRKHEQGENLKNASPDHAGIGNDHFAPKTIYSELYILSCLNLLLIIESNLHYFTRTGKTNKVVESSNERKEILVKLENITKAKNNSIKEKYLIDTTISFLHLVYNYDNLSILDINVKVSEILNNFEALKRFPLLCSILLYIKSDSLNSNSDQKQKSSQVSFNLAKLGYSPMIRYLSGILNGKNATIARNLQQAEIQSQKLKKIKKGFSHRYDKIHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P679</accession>
    <name>A0A1E3P679_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Lipoyl-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_31030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039659.1">
        <property type="nucleotide sequence ID" value="XM_019181792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P679"/>
    <dbReference type="GeneID" id="30199038"/>
    <dbReference type="OrthoDB" id="196847at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050709">
        <property type="entry name" value="Biotin_Carboxyl_Carrier/Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45266">
        <property type="entry name" value="OXALOACETATE DECARBOXYLASE ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45266:SF3">
        <property type="entry name" value="OXALOACETATE DECARBOXYLASE ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0092">Biotin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="2">
        <location>
            <begin position="63"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="140" mass="15705" checksum="0CA874287E6C4DAF" modified="2017-01-18" version="1" fragment="single">FDFAKYSKWLDTVKDEAAALKRLKLDAIDGLTKEEEESLIKWEQEKAQAKESKGSANDFLNDPKAIKVTSHMTANVFKINFKKGENVSLDDTVVILEAMKMEIAVRVKSNKKTKFEVLQVVVDEGDMVNPGDVLMLVKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6D9</accession>
    <name>A0A1E3P6D9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_83213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040125.1">
        <property type="nucleotide sequence ID" value="XM_019185506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6D9"/>
    <dbReference type="GeneID" id="30202752"/>
    <dbReference type="OrthoDB" id="10644340at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="306" mass="36410" checksum="A859A4D44DA63851" modified="2017-01-18" version="1">MAELKYIELKNDVISWTLFISGQLFRYIQFTTSYSLSLIYILIKQFYGIILFSCFPIVKILFLTIKTIWLCLSFTVFIIFEPFNLFIRIIEFNVRKFIDFWLSLLFNTKLEDIFTIEYGKLQFKILVQWVRFINVMIITGLILGILTYYTSVRIIYWMTQVEKKKKKEAEEEKDQGGNVIDDRIRETIFGTFDIDKLEEEHQEQVVDQVDKFWRDEEVERTRLFQDINSTLNDRHEEDDVYNYESLNSLKPQGINESTRSRAGQLFDSMGRESIDSNQGTGTTSIFSRLNLTPITTIEEEEEEQYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6G4</accession>
    <name>A0A1E3P6G4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_28061</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040216.1">
        <property type="nucleotide sequence ID" value="XM_019181444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6G4"/>
    <dbReference type="STRING" id="683960.A0A1E3P6G4"/>
    <dbReference type="GeneID" id="30198690"/>
    <dbReference type="OrthoDB" id="2595934at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR OF PROTEASES PRTT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="18"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ61009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="571" mass="65159" checksum="56AD42EFC2E7CD44" modified="2017-01-18" version="1">MTEPTQIKHVHVPRAAKACLACRRQKTRCFPSNDSNSCLRCQSLSRPCSFLKDGGAESSGGGNASTGSSSNSNRKRSITVIDTSTTIDGVNIIELDKNVKKILTLLQPKTHPITSLNRGEDDSTTTKHKSPFQTSPFTLISNSVSSQSSTIRQLFNPTNIKPTPPISVLTLEIITMKEAITLVDKFKECYGRWISLPNNITTEELINSIQISSPILLTSICVTSLRYQKQSNHDNKLYLDLIKQLKLELFQEISNPINSLEFLKALVILSVYGYSLSVSDFTIDPWFLSGIAVQKFLTLDVDSELLNLDNLHESLDLEQECDKLSNFRIWNHICLVHLVNCVFSSRMCVLDEIRLDQSRKSLDLSQATNFDGRIVSEISLQLLLYNYLQSGSTTNDQVNQFNTDFESFEQELNLWHEQWNYLFRQPTLQFVEFGYHYVYLVAMYHYQFGGDKKIDELDQEDEIKDKEVLKKFMYHSIRLIDHINGIKDIVYFLCLSDQIHLCTMYTTLLFIKLIKWSYKYAGLGQIDMLDLISKAKLIKEKFNQVSRETNDLAFTFATSIQEAIDSVLAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6H6</accession>
    <name>A0A1E3P6H6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_28788</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040236.1">
        <property type="nucleotide sequence ID" value="XM_019181538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6H6"/>
    <dbReference type="STRING" id="683960.A0A1E3P6H6"/>
    <dbReference type="GeneID" id="30198784"/>
    <dbReference type="OrthoDB" id="9994419at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="442" mass="50974" checksum="CD1576F37DFF42DF" modified="2017-01-18" version="1" fragment="single">FKTPQTFDIFLNSLIKNDKLGEYVKVLDFQEFTSIGLGRTGKMNKEIQMVTSTTILNCLKKTPNLIEFLASENIKDDLDSNLLNYLFNQMGNLKAIDFCGCSGEVFVSNFDQLIIKKPGNEYITDVSFHDCTDLHPWILEKIIINLPNLRKLDLTHTQITSTILNLIPYTAQLTHLSLSYCIQLTTRELINFFINHPAITCNKLVWLNLQCDSSTSSPLNNNQLTFLLKMFDCDLVYLNLGGLEISFENLMLIKKKFLNLKSLCIANTSIDIESLVEFLKPPNDEKLITSQRYQQLEFLDLTANRFINRWSIDNPHFLNCCPSLVAFEFSIKIINELEQIGGKLITRNDYNERVVWKTFDSLGRRGWVFKVDDFKNQDNLSKIGLTEYDIDTGRKIFKILKQPDFLKNVNKKISVSKGVLFNADEVFGDLERGIYKYYGMKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6P4</accession>
    <name>A0A1E3P6P4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Guanine nucleotide-exchange factor SEC12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_13167</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Guanine nucleotide-exchange factor (GEF) required for the formation or budding of transport vesicles from the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Golgi apparatus membrane</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the WD repeat SEC12 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040302.1">
        <property type="nucleotide sequence ID" value="XM_019180562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6P4"/>
    <dbReference type="STRING" id="683960.A0A1E3P6P4"/>
    <dbReference type="GeneID" id="30197808"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003400">
        <property type="term" value="P:regulation of COPII vesicle coating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045260">
        <property type="entry name" value="Sec12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284:SF0">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 10" id="KW-0813">Transport</keyword>
    <keyword evidence="2 10" id="KW-0853">WD repeat</keyword>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ61095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="335" mass="36675" checksum="15ABE4A8A3AADBFF" modified="2017-01-18" version="1" fragment="single">VKIESKTHDVGFPVYGAKFFDDKTLVVTGGGGEGNNGIPNKITVLNVDYADSVQLESKKSFEIEGKNDSPTCLDISGNTVLIGCNENSTNIKNGQNKHLRKFKLTEEKEITFDSSADIEETKDPNDYQKFVTISKDGSIAAVVSSKVPSTIKVIDPQTLKVLDVIDEKDEINDVHISPNSKQIAYITDSSLVVVENSEKPILKYDRFAPTYNLTKVKFTKDGDLIVAVNLRNKLGVLLAHVIFEDDALRVKKARVVNSKTQKITALDVSDKLVAVSGNDSSIIIAGLEHFDVYKTLKQVHTFAITKVIFSPNGKYLASTSVANTVNVIKIPEDIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6R6</accession>
    <name>A0A1E3P6R6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_61681</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040318.1">
        <property type="nucleotide sequence ID" value="XM_019183230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6R6"/>
    <dbReference type="STRING" id="683960.A0A1E3P6R6"/>
    <dbReference type="GeneID" id="30200476"/>
    <dbReference type="OrthoDB" id="3980599at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133702" description="Secreted protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="193"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="27012" checksum="5CD44345490382CD" modified="2017-01-18" version="1">MLYKSLLLASLISLSLANPFAEADPLAWADANPYAEAAAEAYAKALAEAMAIAHPDPEAYALAASADDCATIGCHAACGYLIIDGSACSENKTDSYSGPYNTTCLCASDGHFMNRYPSCMECGWTLWKYYGPYVTSALEACSTLSTEPTGTLRCSTTLSQSYTKDANAGCAFGGACVSSSTAGVSNATVTGISNSSSNNTSPSSNGSNGSSVARATSGGDGSTAASSTGSSSGSSASSSASSSGSSSSATNGAQMLVAGTSIFGGLVLALLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6T5</accession>
    <name>A0A1E3P6T5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription initiation factor TFIID subunit 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_17083</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TAF13 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040346.1">
        <property type="nucleotide sequence ID" value="XM_019180725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6T5"/>
    <dbReference type="STRING" id="683960.A0A1E3P6T5"/>
    <dbReference type="GeneID" id="30197971"/>
    <dbReference type="OrthoDB" id="10266074at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07978">
        <property type="entry name" value="HFD_TAF13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003195">
        <property type="entry name" value="TFIID_TAF13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380:SF5">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID/SUPT3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02269">
        <property type="entry name" value="TFIID-18kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="103"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="132" mass="15124" checksum="4FD05368379A430A" modified="2017-01-18" version="1" fragment="single">RKRKKTKFFTNDVRSLLYAFGDVANPLPETVSTLEDILISYVIDTCHEASAYAKTTRRQKIKVEDFKFVLRRDPVKHGRVGELLSLQKVIADARKQFDNSEGKSLKNLNYEKDDDDDKDLDDLDDTANPGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P712</accession>
    <name>A0A1E3P712_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_51876</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040221.1">
        <property type="nucleotide sequence ID" value="XM_019182682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P712"/>
    <dbReference type="STRING" id="683960.A0A1E3P712"/>
    <dbReference type="GeneID" id="30199928"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF15">
        <property type="entry name" value="GENERAL AMINO ACID PERMEASE AGP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="412"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="460"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="492"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="10">
        <location>
            <begin position="54"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ61014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="563" mass="62637" checksum="F5CE5914BCADB5B9" modified="2017-01-18" version="1">MVFSRYSSSKQLDIENEKKADELSITNQSSSDDQGSSTSIHSTHRTLNSRTLNLIAMGGSIGTGLFVTISTGLLYGGPLGLFLSFFIWTGVIFCVTASIGEMVCYLPITAPFIQMAGRCVDEAFEVIVGFNYFIMISVYIPFEITAVNGMIHYWRDDYSPAITFCLQIFIYTMLNLFAVRVFGESEFYLTIGKVALAIGLIFFTFITMVGGNPQHDAFGFRYWQNPGPLVEHLTGGSMGRFHGFMAALNKACFTIVGPEYLSMVAGEAGQETRKVLAGTFRTVFYRLTVFYILGALSVGILVASNDPTLQHLAASDSTSNGSFSPYIIAMNNLKIKVLPHIVNSLCVLSAFSAGNSYVYCSSRALLSISQRGHAPKFFQYCTKSGVPIFCIAVSFMFSLLSLLQLGDSAAKVLTWIVNLVTGAQITNYFFMCITYLGFWNACRVQGLNRSKFQYTSWFQPYTAIFALVLTACMVGAIGYTCFMPGKFTVQDFLTNYLMILLDIVIYIAYKLIKRTKFVNPAEADLVTGLEEVEQHEMNYLEEQERKGKLNRDGWRSKVSGWIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P744</accession>
    <name>A0A1E3P744_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_90591</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040453.1">
        <property type="nucleotide sequence ID" value="XM_019186245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P744"/>
    <dbReference type="STRING" id="683960.A0A1E3P744"/>
    <dbReference type="GeneID" id="30203491"/>
    <dbReference type="OrthoDB" id="1662883at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:supramolecular fiber organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000003">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="15">
        <location>
            <begin position="47"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="16">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="429"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ61246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="449" mass="50021" checksum="5B083E51B531724E" modified="2017-01-18" version="1">MREIIHLSAGQCGNQIGAAFWETICGEHGLDDNGEFHGTEEIQRSKLNVYFNEASSGKYVPRSVNVDLEPGTIDAVKTSKIGNLFRPDNFIYGQSSAGNVWAKGHYTEGAELVDSVLDIVRREAEGCDSLQGFQITHSLGGGTGSGMGTLLISKIREEFPDRMMATFSVVPSPKVSDTVVEPYNATLSIHQLVENSDETFCIDNEALYDICMKTLKLPQPSYDDLNHLVSSVMSGVTTSLRYPGQLNSDLRKLAVNLVPFPRLHFFMVGYAPLTSLGSQSFRSLSVPELTQQMFDSKNMMAASDPRNGRYLTVAAFFRGKVSVKEVEDEMYKVQTKNSSYFVEWIPNNVQTAICSVAPKGLDMSATFIGNSTSIQELFKRVGDQFGAMFRKKAFLHWYTSEGMDEMEFTEAESNMNDLVSEYQQYQDATVEDDEELDYGEEQSLDEVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P779</accession>
    <name>A0A1E3P779_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cation efflux protein transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_90549</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="ODQ61208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_019186241.1"/>
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    <dbReference type="GeneID" id="30203487"/>
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    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098771">
        <property type="term" value="P:inorganic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Putative Cation diffusion facilitator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="entry name" value="Cation efflux protein, cytoplasmic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050291">
        <property type="entry name" value="CDF_Transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840:SF4">
        <property type="entry name" value="CDF DIVALENT METAL CATION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="353"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="397"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="8">
        <location>
            <begin position="252"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="539" mass="60764" checksum="15724C5E58DAC3B6" modified="2017-01-18" version="1">MSKGDHNSNSVVGAGDESTPKPHYSIDPNDGKTKNQNNIHQHSYHALHEYNDNDDGGSPLLKRIKTNERVNSADSHNWGNRRPSFLGLGSSLNLSKENDKLLNYIEKSSRLDTVQELRHLSLIGHIGRYFDWSESYKTDDELKALKNRKLRNFYEDQNELIEKYQEVDKLLDSGIQINMIRNYSDRNDHGEVHRPIPNETYGSIEHEDDDIEPPTTPLHRKDSAVPGNIDLETGLLLGYNKADEIALINFAILVNFAANIALLLGKVVVAVLTSSISIVASVIDSALDFLSTLIIYFSNKLANTKNPKRFPIGRARLEPIGVLIFSIIIIISFLQIGIESIQRLIYPPEAGEIVAIGFTSTVIMVVTILVKVVCYFWCKSIKSSSVAALAQDALVDVVFNTFSIIMPVLGYYFQLYWFDPLGALLLSIYIVVQWSYTCFEHIIHLTGKSADIDDQRVILYLCSRFAEKIHKIKTLNCYHVGDSLNVEVDLILDSNLSIRDSHDLAEALQYTLEALPIVNIERAFVHIDYTVTNYRGHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7G1</accession>
    <name>A0A1E3P7G1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Swi5-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_61925</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SWI5/SAE3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040576.1">
        <property type="nucleotide sequence ID" value="XM_019183277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7G1"/>
    <dbReference type="STRING" id="683960.A0A1E3P7G1"/>
    <dbReference type="GeneID" id="30200523"/>
    <dbReference type="OrthoDB" id="3978967at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032798">
        <property type="term" value="C:Swi5-Sfr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034974">
        <property type="term" value="C:Swi5-Swi2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010772">
        <property type="term" value="P:meiotic DNA recombinase assembly involved in reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000709">
        <property type="term" value="P:meiotic joint molecule formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010760">
        <property type="entry name" value="DNA-repair_Swi5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28529">
        <property type="entry name" value="DNA REPAIR PROTEIN SWI5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28529:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN SWI5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07061">
        <property type="entry name" value="Swi5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="98" mass="11218" checksum="217C2B911B5DCEBC" modified="2017-01-18" version="1">MYRARSQSATSPKAIQERINTKSKELEELQAESANLILELEHQTQLDPEEIVKAHILGLKKYNELKDVALGLITMIADQRQLRMADILKEMNIEGDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7S3</accession>
    <name>A0A1E3P7S3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal protein L37</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_62029</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the eukaryotic ribosomal protein eL37 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040678.1">
        <property type="nucleotide sequence ID" value="XM_019183298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7S3"/>
    <dbReference type="STRING" id="683960.A0A1E3P7S3"/>
    <dbReference type="GeneID" id="30200544"/>
    <dbReference type="OrthoDB" id="10259236at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000448">
        <property type="term" value="P:cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.30:FF:000001">
        <property type="entry name" value="Ribosomal protein L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00547">
        <property type="entry name" value="Ribosomal_eL37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001569">
        <property type="entry name" value="Ribosomal_eL37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018267">
        <property type="entry name" value="Ribosomal_eL37_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003214">
        <property type="entry name" value="PRK04179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10768">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10768:SF0">
        <property type="entry name" value="RIBOSOMAL PROTEIN L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01907">
        <property type="entry name" value="Ribosomal_L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01077">
        <property type="entry name" value="RIBOSOMAL_L37E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ61471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="95" mass="10813" checksum="B595CB96FB6B6A57" modified="2017-01-18" version="1">MSSRIPIIKKGTPSFGKRHNKSHTLCNRCGRRSFHIQKKTCAACGYPSAKIRSHNWALKAKRRRTTGTGRQSYLKNLPRRFKNNFQTGVAKPQSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7T0</accession>
    <name>A0A1E3P7T0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">KRR1 small subunit processome component</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">KRR-R motif-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_28011</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. Essential for vegetative growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Interacts with snoRNA U3. Interacts with MPP10, KRI1 and with ribosomal proteins RPS1A, RPS4A, RPS4B, RPS8A, RPS8B, RPS11A, RPS11B, RPS13, RPS24, RPS25, RPL4A, RPL7B, RPL8, RPL23, RPL25 and RPL28.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
            <location evidence="1 11">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the KRR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040670.1">
        <property type="nucleotide sequence ID" value="XM_019181438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7T0"/>
    <dbReference type="STRING" id="683960.A0A1E3P7T0"/>
    <dbReference type="GeneID" id="30198684"/>
    <dbReference type="OrthoDB" id="441223at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22393">
        <property type="entry name" value="KH-I_KRR1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22394">
        <property type="entry name" value="KH-I_KRR1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000011">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000014">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041174">
        <property type="entry name" value="KRR1-like_KH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048550">
        <property type="entry name" value="KRR1-like_KH1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048548">
        <property type="entry name" value="KRR1-like_KH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048549">
        <property type="entry name" value="KRR1-like_KH2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024166">
        <property type="entry name" value="rRNA_assembly_KRR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581">
        <property type="entry name" value="HIV-1 REV BINDING PROTEIN 2, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581:SF0">
        <property type="entry name" value="KRR1 SMALL SUBUNIT PROCESSOME COMPONENT HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17903">
        <property type="entry name" value="KH_KRR1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21800">
        <property type="entry name" value="KH_KRR1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006515">
        <property type="entry name" value="KRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 11" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="6 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 11" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="K Homology" evidence="13">
        <location>
            <begin position="122"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="285"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="285"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="345"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ61463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="357" mass="41988" checksum="5366076A76CD64B2" modified="2017-01-18" version="1">MPSTHNRDKPWDTPDIDKWKIDEFKPEDNISGLPFAEESSFMTLFPKYREIYLKQIWPDVTKALDKHHIACTLDLVEGSMTVKTTRKTFDPAIILKARDLIKLLARSVPFPQAVNILRDDIACDVIKIGNFVTNKDRFKKRRQRLVGPSGQTLKALELLTECYILVQGHTVSAMGPFKGLKEVRRVVEDCMKNIHPIYHIKELMIKRELRKNPELANEDWSRFLPQFKKRNVARKKPKQVRDDMEKKKEKKVYTPFPPAQLPRKIDLQIESGEYFLSKKEKRVKELDAKKEMQEEHKITREEERAKDYVAPEEDTYENPLLKEKKSKKSKKRSSEDNDDEDGEKKKKKKKKSKHSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7W0</accession>
    <name>A0A1E3P7W0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA repair protein RAD5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_91048</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040713.1">
        <property type="nucleotide sequence ID" value="XM_019186273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7W0"/>
    <dbReference type="STRING" id="683960.A0A1E3P7W0"/>
    <dbReference type="GeneID" id="30203519"/>
    <dbReference type="OrthoDB" id="2801544at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042275">
        <property type="term" value="P:error-free postreplication DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010994">
        <property type="term" value="P:free ubiquitin chain polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014905">
        <property type="entry name" value="HIRAN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF22">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08797">
        <property type="entry name" value="HIRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00910">
        <property type="entry name" value="HIRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="512"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="877"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="963"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="369"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="40"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="58"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="369"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="388"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ61506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1131" mass="129780" checksum="815D3C598158FFC3" modified="2017-01-18" version="1">MEDESRFFGDSSAGEIQVKREHLDPIEHHKELLRQSSANSTLQSEPTSSSSQPESGKQDNPNEVNISSKQSLFVANDEEEYQSDPEEDAYMFFESQVQAIIEGITPPEVQQLYIKFKDHEQPVISAVNQYYDGIVFDPEPKPQAQPQPRRTISQQPTITSAVRSNQTNLPQRKRRSVVPTQTKELSPSVKWRRFIGSLQVVGWATRPTVKPLPYGTQLTIVKNTSQSTSKKRRSGGTGSNVSNIKLVQDYHDPHKAKEIGRVPEDIAAILFPLVDQDIVDFDVTVVLCDGRLSTGDSFIVQLDCYLTSKIFEKWKNAASFEDELKQMKRKQSSFYNVGYETEVEQHVRQRQIGLFKLFNKLDFQVRTTDDDEEEDEVIDLDDDDSMDNITPTQQNNGEPKPADELNLNQLKEFYRITQTADVLTTLPETETKENFKLNLRPYQKQGLTWMLRGEREIINEDEGEQMNPLWKEFKWPKERSWAVMRNESLRQSFDTNVFYANLYSGEFSLEKPILKTMCRGGILADEMGLGKTVSTLALIHNAPFDKDYDVQNAIEERYASRTTLIVVPTSLLSQWQDEFNKANNSDGSVFVYYGTESGKDLKAQLCGDSPPMVVLTTYGTIQHEWSKLIDYASKQDNVLPKLGLFSVRFFRVVLDEGHNIRNRMAKTTKACYDLQSSRKWLLTGTPIVNRLDDLYALIKFLELEPWSNISYWKTFVTIPFENKKYKQALDVVQSILEPILLRRTKSMKKDGVALVELPPKEVVIERIKFSPKEHKLYSWFLAKASSSVKASIAKGDILKRYTTILVHILRLRQICCHMDLINGGSDEMDEDISTKQVQQIEVPEELLKRSETFTINDLNLASSNIYNMFDKIDDLECSICTNQPIPIDQLAFTECGHPFCVSCILEHCDYQEMKHNEILCPNCRQEISSAKLISVKNIPSVLSNKSYTLSKFDNSLRSSKLNALLSHLRIIRDTTSNEKVVVFSQFSTLLDIMERELHMENGLTVFKFDGRLSMNNRSNILKEFKQPRVGVTVLLLSLKAGGVGLNLTEASRAFMCDPWWSPSIEDQAIDRIHRIGQENNVKVVRFIMEGSIEEKMLKIQERKRTIGEAVEAEEEERRKRRVEEIEMLFEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P828</accession>
    <name>A0A1E3P828_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_75777</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040773.1">
        <property type="nucleotide sequence ID" value="XM_019184259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P828"/>
    <dbReference type="STRING" id="683960.A0A1E3P828"/>
    <dbReference type="GeneID" id="30201505"/>
    <dbReference type="OrthoDB" id="3980749at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061499">
        <property type="term" value="C:outer plaque of mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902412">
        <property type="term" value="P:regulation of mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031028">
        <property type="term" value="P:septation initiation signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052574">
        <property type="entry name" value="CDIRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47566">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47566:SF1">
        <property type="entry name" value="PROTEIN NUD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="705" mass="80764" checksum="75F8C4148D6ABBDB" modified="2017-01-18" version="1">MTTMQQSPTKQITERLQRLNLYSGDEPQSRNVRSQSETLTSAKNHLHIPKLIFNQDFADESRVGENTDIFRSPDSGAADIDSDATVDEGDTVVHNKENADTTLPDWMAVKLKMQSSTTTKKNPAKPKLINRNKEFRSTHLKPKLVSSVTDSESESEIFKNETWEEMKKQYDIGGFDDDDDDKSIEESGGVDDTITEARNLNDTSAYSFQDMNNLRIISDAQTSSNGTKKVTSFKSDYIDSPMAAPKILKSFKQQTKPSYTETDDKANDKVEIKLITPVEMNMKFDPDDGKWVSPEQVHEPKPKYDDILVIEEEIKKPVIKILEPTPDNTMKTTLDIDPQQITNVSQLDLSFSESRKTLISALTSVIKPKTDWDKVDEVDLSHKRLITVKSLEEFLPKLLEINLNFNNLTTIEGLSSNLQSVSISHNKISDHFLNFKQFQYLHKIDISFNKLTNMKLFQNLRNLRELNLSNNDIKDFVELPSLQVLNVSNNEINGELNFDNFKFPNLEVLDLSGNKLNKVDMTGLKQLRILKLKNNENLQHLEIEENSTKLKKLNLVGLKKLSKFECLDKLQSLRSLSIEGTTLVKGNFPRLETLKVINDKKLENLFNGSIKVNKHLLSITLEECSIDSKVLLNMNKFHSNFPHLQRISLENNEIKDNYLNLVVFFQKFKNLQKIKLDNNPIVDELKNEQDKRLFKLMIKKLTSDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8G1</accession>
    <name>A0A1E3P8G1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histone deacetylase complex subunit SAP30 Sin3 binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_27779</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAP30 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040364.1">
        <property type="nucleotide sequence ID" value="XM_019181407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8G1"/>
    <dbReference type="STRING" id="683960.A0A1E3P8G1"/>
    <dbReference type="GeneID" id="30198653"/>
    <dbReference type="OrthoDB" id="510958at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061188">
        <property type="term" value="P:negative regulation of rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061186">
        <property type="term" value="P:negative regulation of silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016479">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000219">
        <property type="term" value="P:positive regulation of invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024145">
        <property type="entry name" value="His_deAcase_SAP30/SAP30L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038291">
        <property type="entry name" value="SAP30_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025718">
        <property type="entry name" value="SAP30_Sin3-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13286:SF6">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT SAP30L-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13286">
        <property type="entry name" value="SAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13867">
        <property type="entry name" value="SAP30_Sin3_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase complex subunit SAP30 Sin3 binding" evidence="8">
        <location>
            <begin position="107"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="8"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="162" mass="17999" checksum="67FD67555589C903" modified="2017-01-18" version="1">MAIRGSSKEAPSASESEAKNSTSHNNNNSTNSRASTKARNAAAQAAQAELLARHIHSNGPKEQKSVDPLNFESLPDITLRKYRDHYNLPAKSSLSTDGYLLASEIGKKSYSYKNRDRVTKPELASAVKKHFMSQTVKESEIITNFIYKVNNQDKAFKLNLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P986</accession>
    <name>A0A1E3P986_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase PEP5</fullName>
            <ecNumber evidence="9">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_37852</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex. Component of the class C core vacuole/endosome tethering (CORVET) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Vacuole membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the VPS11 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="9"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041158.1">
        <property type="nucleotide sequence ID" value="XM_019182529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P986"/>
    <dbReference type="STRING" id="683960.A0A1E3P986"/>
    <dbReference type="GeneID" id="30199775"/>
    <dbReference type="OrthoDB" id="26184at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033263">
        <property type="term" value="C:CORVET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036205">
        <property type="term" value="P:histone catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035542">
        <property type="term" value="P:regulation of SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032889">
        <property type="term" value="P:regulation of vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099022">
        <property type="term" value="P:vesicle tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16688">
        <property type="entry name" value="RING-H2_Vps11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057308">
        <property type="entry name" value="CHCR_PEP5_VPS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057307">
        <property type="entry name" value="PEP5_VPS11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016528">
        <property type="entry name" value="VPS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024763">
        <property type="entry name" value="VPS11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323:SF24">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23341">
        <property type="entry name" value="PEP5_VPS11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23356">
        <property type="entry name" value="TPR_PEP5_VPS11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12451">
        <property type="entry name" value="VPS11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17122">
        <property type="entry name" value="zf-C3H2C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007860">
        <property type="entry name" value="VPS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0926">Vacuole</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="CHCR" evidence="11">
        <location>
            <begin position="397"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="870"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="604"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="827"/>
            <end position="861"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR007860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ61951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="962" mass="108855" checksum="0CCE114D9BA8E8DA" modified="2017-01-18" version="1">MSWRQFQFFEAIPIRDPNLGSESPLYSDPSLTATCATPDYLIISTNNSVIKFIDKDLKLIKSFTAYEKGWSITFIRYIEGTNFFTTVAERQGQPSILKLWSIEKIITKQPDKVNDSSYHSIVHIKNGSNSYPITSFTFSSNFSILAFGFANGNVVLVRGDILRDRGSSQTIAYRSQDPITGLELVANNNYDPLLYITSTSKILSVPTTGGNNNRVEKWLEKSKGADLGTVDIDGDQLIVGRTEGIVFYDTNGSSYTIALDLPKRLIHKFGKYIVIVTTNNTTSSSLVLNGYSEPTKIIIANIEQKLISFSYTISSTVSDIFSLWNDIFLLSSDGILYRFHEKELQEQLNIVVKRDLYPVAIELAQNNIDDQVVLDIKRKFGDYLYSRNETTEAMDQYIDTLSLGKTSEIIKKFKDSKEVSNLARFLEEMLKQGLCTKEHVTLLLCTYCKLKDIEKLNSFIDKYDQETNELVKVEFDLEVVVELCRETKFLQQASKLAQNSGYSSLAVDILLRDLRDAPAAIQYIKTLPINETLRILVEYARLLLESIPNATTALLIDVFTGKYKPEASTSNTLLGEEKTDTPVLLQSYKAFVNYMSTAASTITASSSEDDLQNGDLNGSEPTYQPPKPRVIFPSFVNRPNEFVIFLEACAVTYDAFEGNEKDKADILNTLYELYLTLGTNEEKEEDREAWETKAIELAKSDPKLIDPTSILLISNIFDFNDGQILAREGPGFEIDLFRSCVASGDVEGAIDTLQKYGDNEPELYPLALMFYTSSENIFEKVGEEKFKFVLDKIKKDRILSPLEIIQALSVNKIATIELLKDYILEFVETEKKEIEINEKLIDSYRQEANKKKTEIHKLESSPKTIQPNKCDSCSNKLDSETVHFMCSHSYHKHCLAEVDKCPKCTPARETVRNIREGQRIIGERNDLFNATLEDTDNRFKVVTDFFGKGAMDHANYVISNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9S0</accession>
    <name>A0A1E3P9S0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Ribonuclease H</fullName>
            <shortName evidence="12">RNase H</shortName>
            <ecNumber evidence="5 12">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_87375</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 12">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the RNase H family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="5 12"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041370.1">
        <property type="nucleotide sequence ID" value="XM_019186105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9S0"/>
    <dbReference type="STRING" id="683960.A0A1E3P9S0"/>
    <dbReference type="GeneID" id="30203351"/>
    <dbReference type="OrthoDB" id="407198at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.970.10:FF:000002">
        <property type="entry name" value="Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.970.10">
        <property type="entry name" value="Ribonuclease H1, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_bL9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050092">
        <property type="entry name" value="RNase_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017067">
        <property type="entry name" value="RNase_H1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011320">
        <property type="entry name" value="RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037056">
        <property type="entry name" value="RNase_H1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10642">
        <property type="entry name" value="RIBONUCLEASE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10642:SF26">
        <property type="entry name" value="RIBONUCLEASE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01693">
        <property type="entry name" value="Cauli_VI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036852">
        <property type="entry name" value="Ribonuclease_H1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="L9 N-domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="13">
        <location>
            <begin position="159"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR036852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ62163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="330" mass="37524" checksum="4366D5C5DE4FA2E4" modified="2017-01-18" version="1">MKSNSKKAFYAVAKGQKTGIYDSWDRCKAQVSDYEGAKFKKFDTQHEAKDFIEKNKETGSISKTEAGRKLAQAKEKTSYYGIVYKNGESKIVKTWAECAKATKGIQGAAFKRFNSLKDAEAFVSQGENGQPPLSPNSREVRTKLFLEHNSKQLLKEQQSLKPQIIFCDGSFIPQRSGKDKAGFGVYYGPGDSRNVAVPITTEPKNSFISEVKATAHVLKQICKEIDRYKKEDLEIIPKYSISSDSETVKNILHQYADTWTDKDYEKHREVPELKEMVHDYKKVRSFYNDNSPIFDDHKFGITWVKGHVGIEGNEQADRLAREGAEQSIRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9T6</accession>
    <name>A0A1E3P9T6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SURP motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_75853</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040854.1">
        <property type="nucleotide sequence ID" value="XM_019184272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9T6"/>
    <dbReference type="STRING" id="683960.A0A1E3P9T6"/>
    <dbReference type="GeneID" id="30201518"/>
    <dbReference type="OrthoDB" id="447637at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.790:FF:000002">
        <property type="entry name" value="Splicing factor 3A subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
        <property type="entry name" value="Surp module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045146">
        <property type="entry name" value="SF3A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022030">
        <property type="entry name" value="SF3A1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15316">
        <property type="entry name" value="SPLICEOSOME ASSOCIATED PROTEIN 114/SWAP SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15316:SF1">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12230">
        <property type="entry name" value="PRP21_like_P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="SURP motif" evidence="8">
        <location>
            <begin position="20"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="SURP motif" evidence="8">
        <location>
            <begin position="115"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="378"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="378"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="401"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="418" mass="48527" checksum="DA8CA5F8FC697512" modified="2017-01-18" version="1">MSADEIPDDVIIPPPAIKEIILKTATYVHRNGKEFETKIRENESNNNKFTFVNENDPYNKYYNFVLDNIETFIEVSGENGSADAEEQHEDEQVEPPLELDFLVELPPITSKDLEIIKLTALFVAKNGENYINQIKNKYKDQTAQFSFLNDSHSFHQLFLSFLKQYRKLINSKEDIDLDHDVLEESYKRAKFNELNKINTVKSKELEEAKKLKYASIDWQDFIVVETINITDIDSHTEFPLPLTIADLQYRSLEQKSQDISIPSVTPIADTKETSAKPMKIRSYGETRLKRGQQSQGEKLVECPITGKLIPESNFERHMQILLRDPKYKQEKLNYESKIKNSNLSTSEVVNNIKNLFKPKNNGDRENSTKRQKVQWDGYQSSVNFVKQTNHNDGLSNDEVEELKRKRQELENKIGPSRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9X8</accession>
    <name>A0A1E3P9X8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_102633</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040959.1">
        <property type="nucleotide sequence ID" value="XM_019180362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9X8"/>
    <dbReference type="GeneID" id="30197608"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="486" mass="57629" checksum="CB81325DE680A9FF" modified="2017-01-18" version="1">MALGNYPVHLSELAGYSKEELSTMLVEFSRSNARMKDEIASLNQNLQNAVIYQQQREANFGLIVRSFEYTLQFRDQWIRELRAERWKQQCRLDELCFVAAFSSDDESRDDDEYLNIHKRRKMREEHHQSSNDEAIHSTVGKESEDEEDVVRHEREKENEVEQEVEEVENEVDVEEEEEHEVEEDEEEVVEVEEDEEDEEEVVEVEEDEEDEDEKEEEEEEEEEIQIHGYGICHEEVVVPMLKDISHIKDLVKSGTHFPNLKDCLLNCFTYYGLYLKFPFFCDQKSRTANEKNQTISNTRYYSCCCCRSRLFLVTIPKKEAGCTLTLLSERKHECDMEDLYTNYRDYYTNKKKLAPKTIENKVELLVDMGDAICDEITYIWAFSILEKFIPPELDFQLEQIINIVKFHKELFISDHISSDEQIEAIKDVSLDVFLSINSLDDHELFRCTNRVFCRSNSCEQTKENYKQQAQKIFAKLLHLNMLRKHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA02</accession>
    <name>A0A1E3PA02_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Srp40 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_25991</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041337.1">
        <property type="nucleotide sequence ID" value="XM_019181214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA02"/>
    <dbReference type="STRING" id="683960.A0A1E3PA02"/>
    <dbReference type="GeneID" id="30198460"/>
    <dbReference type="OrthoDB" id="5599646at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039191">
        <property type="entry name" value="Nopp140-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007718">
        <property type="entry name" value="Srp40_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23216">
        <property type="entry name" value="NUCLEOLAR AND COILED-BODY PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23216:SF1">
        <property type="entry name" value="NUCLEOLAR AND COILED-BODY PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05022">
        <property type="entry name" value="SRP40_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Srp40 C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="55" mass="6069" checksum="12CCDBAF3234DF98" modified="2017-01-18" version="1" fragment="single">DNTYKGAAGTWGEKANERLGMVRGKDFTKNKNKMKRGSYRGGSITMASGSYKFTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA63</accession>
    <name>A0A1E3PA63_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Complex 1 LYR protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_24941</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the complex I LYR family. SDHAF1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041512.1">
        <property type="nucleotide sequence ID" value="XM_019181077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA63"/>
    <dbReference type="STRING" id="683960.A0A1E3PA63"/>
    <dbReference type="GeneID" id="30198323"/>
    <dbReference type="OrthoDB" id="273010at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd20268">
        <property type="entry name" value="Complex1_LYR_SDHAF1_LYRM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045295">
        <property type="entry name" value="Complex1_LYR_SDHAF1_LYRM8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13675">
        <property type="entry name" value="LYR MOTIF-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13675:SF1">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="5">
        <location>
            <begin position="13"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="82" mass="9801" checksum="3CF8F90BE27F4C14" modified="2017-01-18" version="1">MAAKIRKLSGLQKEVLKLYRLCIRASYTKPKENRQHFVSYTKNEFAKFQQLPKKEFSTIEHLLRTGYKKYEMFSAPEIKDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAD1</accession>
    <name>A0A1E3PAD1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Probable electron transfer flavoprotein subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_26509</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </cofactor>
        <text evidence="7">Binds 1 AMP per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="7">Binds 1 FAD per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ETF beta-subunit/FixA family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041586.1">
        <property type="nucleotide sequence ID" value="XM_019181271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAD1"/>
    <dbReference type="STRING" id="683960.A0A1E3PAD1"/>
    <dbReference type="GeneID" id="30198517"/>
    <dbReference type="OrthoDB" id="276685at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000011">
        <property type="entry name" value="Electron transfer flavoprotein subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000049">
        <property type="entry name" value="ET-Flavoprotein_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294:SF8">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01065">
        <property type="entry name" value="ETF_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="8">
        <location>
            <begin position="27"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00070315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="255" mass="28073" checksum="6C6781449B1930C3" modified="2017-01-18" version="1">MSTKLNILVPVKRVIDFAIKPRINKTNTGIEKAGVKFSINPFCDIAVEEAVRIKEANKSLVNKIHVVSIGPTKSQDILRQSLAKGADSSTLVDVGEEEIEPLSVAKILKQVVEKNQSNLVILGKQAIDDDSNHTGQMLAGLLKWPQATNAAKVELKGEEVFVTREIDGGEETVKAKLPLIITTDLRLNEPRYATLPNMMKAKKKPLEKLKLSDFDVDVTKRLEVLKVEEPPVRNAGIKVGNVDELVSKLKDLKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAE4</accession>
    <name>A0A1E3PAE4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YAP1-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_60421</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041568.1">
        <property type="nucleotide sequence ID" value="XM_019182988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAE4"/>
    <dbReference type="STRING" id="683960.A0A1E3PAE4"/>
    <dbReference type="GeneID" id="30200234"/>
    <dbReference type="OrthoDB" id="5396786at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013877">
        <property type="entry name" value="YAP-bd/ALF4/Glomulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040347">
        <property type="entry name" value="YBP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28020">
        <property type="entry name" value="YAP1-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28020:SF1">
        <property type="entry name" value="YAP1-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08568">
        <property type="entry name" value="Kinetochor_Ybp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="633" mass="73110" checksum="687237D1EE169C7C" modified="2017-01-18" version="1">MSDEEQLPVDTVCEYIENAIVELKETGDYLSYATLLDIHLSDPERYNDEEKLQLLNKLHDILSENLDVVREIGWDLPELLINFYDLEWDYTGSLLRETGLIKAIMDNFDLVAKNGNAKELFLKSIELLTTLEDFDASNEKSFKIADIKLHTLIELLNTSLKRINTIYPSRFLSMATASLLRAITMYCTKSIHTRFVLRRLYAFARDYMAPLKPNDYLSKHGITQEEADKIEDDENFLQRKLLQSFLTQVSEITFLQNSFDLSVELYKSLQYKCQKKLPPPNESDEEAITSSRGLFTRVTQLALSFDLDLADDFEKLKKSSVDLFEKIDLKKDDDEKMQDILKFAVNDKISHLYHPESKEIPFNLAGELNLITMYTREISHVFPITIREAVAITLRLLSPGLISEKFQNKGSIDTCLFWCWVAIISSKQKDFQLIPHHELILFLQILIYYASTHDRPSVRLVLYTLLTRVLTTIDEDTSYNFLVDTLTTAPFLNAKAAVVTILKDLIVRVKPTDIDALTDELEKSHLENDERKPKLPERHYIELKNDRIDDIYALIRESIDETFHGEELNPASFKVTLGYLNLLITIKDRFPDDKIKEIVKVTKKNIENLKDEQSSDDPTVNVNFISMALESLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB44</accession>
    <name>A0A1E3PB44_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_26377</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041304.1">
        <property type="nucleotide sequence ID" value="XM_019181254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB44"/>
    <dbReference type="STRING" id="683960.A0A1E3PB44"/>
    <dbReference type="GeneID" id="30198500"/>
    <dbReference type="OrthoDB" id="167718at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF51">
        <property type="entry name" value="NUCLEOLAR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="74"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="168"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="208" mass="23937" checksum="893CD29A7B7CD118" modified="2017-01-18" version="1">MAEQEVKLTKKQKKALEFRNKTKKDKTEESKPENKDDQIEKVPSNETNEDGEPKKKRKTRRGKKGKGSNSAPRFLLFVGNLPYDINETELLAHFAAANPDRIRIRTEKGIAFLEFDGSGRDIQHKMEVALRMHRSTLRNRKINVELTVGGGGNSNNRLEKIREKNTKLLDERKQRLKEERAEKDKKKAAPTPEEMGNVHPLRAKMIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC29</accession>
    <name>A0A1E3PC29_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_26843</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041644.1">
        <property type="nucleotide sequence ID" value="XM_019181307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC29"/>
    <dbReference type="STRING" id="683960.A0A1E3PC29"/>
    <dbReference type="GeneID" id="30198553"/>
    <dbReference type="OrthoDB" id="6339427at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015798">
        <property type="term" value="P:myo-inositol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015791">
        <property type="term" value="P:polyol transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000134">
        <property type="entry name" value="MFS sugar transporter protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="379"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="437"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="471"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="498"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="97"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="561" mass="61740" checksum="D9632BFC22A74A59" modified="2017-01-18" version="1">MSSGLFEKTTEKGVEDGQVSYNTSDSPSVQPKDVQIDSTHDKITKLQLDPNFNFDKELEELEILAANYKADRTALQRLCGLEVELNFKNKKNMVILLGVFSAAAGLLSGLDQSIISGASIGMNKMLHLTEDEASLVSSLMPLGAVLGAIMMAPLSTYLGRRTALIISCIWYTIGSALCAGSMSHHMMYAGRFLLGIGIGIEGSIGMYISESCPARVRGNIVSLYQLNIALGEVIGYAVAAMFYEVHGGWRFMVGSSLLFSTAVFIGLFFLPESPRWLVHKGRTGEAFAVWKRLRDINDPQNILEFLEVKQAVAQEHEREQHETTLQRWSELFLVPRNRRALFYAVVMILLGQITGINAILYNMSSLMSKIGFDEKKSVFMSLVGGGALLLGTIPATVTKKGRRIWSMNLAGCFVGLALVGGGYLIDPIKHKSAALGVYLSGLILYMSFFGSYACLTWVVPSEVFNTKTRSIGMSICSALLYLISFAVTYNFAKMKKAFTYTGLTLGFYGGIALIGLIYQIIFMPETKDKTLEEIDDVFSKSPFTIARENIVNIKRKIGKQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCF6</accession>
    <name>A0A1E3PCF6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">beta-glucosidase</fullName>
            <ecNumber evidence="3 6">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_51037</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Glycan metabolism; cellulose degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="3 6"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042248.1">
        <property type="nucleotide sequence ID" value="XM_019182660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCF6"/>
    <dbReference type="STRING" id="683960.A0A1E3PCF6"/>
    <dbReference type="GeneID" id="30199906"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="UniPathway" id="UPA00696"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.300:FF:000006">
        <property type="entry name" value="Beta-glucosidase H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000495">
        <property type="entry name" value="Periplasmic beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050288">
        <property type="entry name" value="Cellulose_deg_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019800">
        <property type="entry name" value="Glyco_hydro_3_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715:SF27">
        <property type="entry name" value="BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07691">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00775">
        <property type="entry name" value="GLYCOSYL_HYDROL_F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA14" evidence="7">
        <location>
            <begin position="395"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ63041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="836" mass="91949" checksum="17280CFCE062FD0B" modified="2017-01-18" version="1">MAEKFDVEDILEKLTTNEKIGLLSGVDYWSTYPIHRLNVPSIRTSDGPNGIRGQHHFNSTPTAAIPVGTALASTWNTELLHQVGEFLDLEARAKGVHVLLAPTVNIQRIPNGGRGFESFSEDPLLSGKLAASYINGFQNKGLAATIKHFVGNDQENKRFSSDSVISQRALREIYLKPFEIAIEESNPKALMTSYNKVNGVHVSEDEYLLKTILRGEWNWNGLVMSDWTGTYSTSDALNAGLDLEMPGPGRWRGERVSHSLLSNKILESTLDERALNVLNLVKLVKDSGVPENAPEAERNAPEDRDFLRKVAGEGIVLLKNDQNVLPFQKKRTLVIGPNALYAAFSGGGSALATPYYAVSPIQGIKEKIGDENVVFNIGAYNHKFLPSLKHELYHEGVNGTLFKIYNEPPEDTSRVPFDTLVLDDVNYFRMNDYVHSKLKSDKFYAELVSSFIPAFDGVYEFGLAVFGTAKLFIEDEIVVDNATKQELGESFYGLGTPEIKGKFKVTSGKHYKLVIQYGSGATSDVAKGANKTFYGGGGLRFGGALVIGPEKEIEKAAANAKLFDQVVIITGLNKDWESEGGDREDLKLPGYSDKLIEAVLDANSNSVVLIQAGTPVELPWVSKASTVVYTSYGGDETGNAIADVLYGDINASGKLPLSFPTRFEDTPSFINNTTNNGKVLYGEDIYVGYRFYEKVDKAVVFPFGHGLSYTRFELSGLKLNIVEDQLSVSLTIKNVGSYGGAEVVQLYVGSKKSNIQKPVKELKAFTKVELKQNVKSDISFKLNLGKSTAFFDESKNKWVSEKGEYFVYVGTSSSDLKALKADFSIEKTTSWLVPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCU6</accession>
    <name>A0A1E3PCU6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_67213</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042241.1">
        <property type="nucleotide sequence ID" value="XM_019184074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCU6"/>
    <dbReference type="GeneID" id="30201320"/>
    <dbReference type="OrthoDB" id="2213137at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF315">
        <property type="entry name" value="TRANSPORTER MCH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="52"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ63034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="480" mass="53105" checksum="B4260FE1DD832012" modified="2017-01-18" version="1">MPSMNPTTSPMTIKDGKENEVSTTTIEQSRAQEFNGAFNEDDYVDVPPDGGYGWLCCACVTAMNFATWGVNCSYGIFLSFYLKNDVFPKATPTDYALIGGLIVFLTLILIPYSAVSLIKFGYRVTASIGIIIQLAGYIGASFSTTIGQLYVTQGILIGISYGFIFGANSIVLPSWFLKKRALANGISHFGNGLGGVVFTLAINKLIIETGDQKWALRVLAIVSFVICTVAMLLVKIRIPQNSSFKEPNNNTTMKIFRNIFDYKVWKSFPLQLATIWTSIGTTGYVICLYSLSNYTLSMGFSQHQAMVVTVVLNASQAVGRPMIGVLSEKCGRVNFTTFATFFTCIIVFVFWINLKSYGALIIFTILLGFFVGISSVNVVPLVVDVVGLNSFASGLGWANSFAAIMSLVSEVIALNLRDYSLVDRNPYFHCQIFVGAMYFVAFLVLIPYREWRIKRMINQLKNSKNLTESEREQIFTKSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PDF5</accession>
    <name>A0A1E3PDF5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein YAE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Protein yae1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_60953</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the YAE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042114.1">
        <property type="nucleotide sequence ID" value="XM_019183085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PDF5"/>
    <dbReference type="STRING" id="683960.A0A1E3PDF5"/>
    <dbReference type="GeneID" id="30200331"/>
    <dbReference type="OrthoDB" id="20086at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019191">
        <property type="entry name" value="Essential_protein_Yae1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038881">
        <property type="entry name" value="Yae1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18829">
        <property type="entry name" value="PROTEIN YAE1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18829:SF0">
        <property type="entry name" value="PROTEIN YAE1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09811">
        <property type="entry name" value="Yae1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Essential protein Yae1 N-terminal" evidence="9">
        <location>
            <begin position="57"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
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    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007096"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00017286"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF09811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ62907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="166" mass="18822" checksum="F7935106251BF40E" modified="2017-01-18" version="1">MGKKEAQDSEISDSEIWADSDEEFRPNVPDDEFYGEKSGVLENSEISALRRVHAKKGYLDGLSTAKEDSLQEGFDKGYPLGASIGIDVGKVISKLQFISTMNEIDPKLKDQAKTYLESAKEELLIQKVLNRRYFDGDLKLPEDLHSLLQRWKSDIDDLQAQIKLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTY6</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">WICANDRAFT_65892</name>
    </gene>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454236">
        <property type="protein sequence ID" value="ODQ56639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019184010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTY6"/>
    <dbReference type="GeneID" id="30201256"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="453"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <end position="53"/>
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            <end position="451"/>
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    </feature>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="549" mass="61624" checksum="09C29A01164C23EC" modified="2017-01-18" version="1">MAVTRNSNRRNTSSTSSTSSTTRVTKTRKPARKTKANPRVTAIENNISASHAASTSENTPNSTTSEENFSTNGAQQAALSAQNENFSNSTETDHETNGSNQNRTFFPPPGLPQPNSAPLFPPGLSAPSSLPPGLSNNTQLGTNSTNSNANAATNVLNSMDPQTLAQLLQLLQAVQQPLQQQNHNDSPINIITTLKDKCFKQIPNGFSQAWYYCLVNKLPTSDSEWLNLPNPFYTPLDINTITKLYPAAYEIINKHLITTLSSTLNFQYLYNKLGRDTHPSKAQEYLDLVATVHVPNVIHPIYNSAVKLTNNFEIPNDFLSFLKSREKSIEEFKTKHQNNLVAALETVITLMLFNQERKFFPESDKFILKFIKSIFHEKGEITYGLLLEKFLHEGKENVLNVNRNNDMVKINTVQVDFETPVSYDKNQNKSRQWTSSKFSDQKPASSTTKPFTNPKFANLIRTEGPEGGNYFKYYHSIPSDIGHSSIGDKAPYCHHCGIRHEINQHVFDLTGKVSSLEKFQQQLTQLLKYKPQGHQETIAKTLKLINENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU46</accession>
    <name>A0A1E3NU46_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_107855</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ56648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3NU46"/>
    <dbReference type="GeneID" id="30197758"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
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            <end position="89"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        </location>
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        <location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
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        </location>
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        </location>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
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            <end position="319"/>
        </location>
    </feature>
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        <location>
            <begin position="364"/>
            <end position="386"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ56648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="386" mass="41593" checksum="EFFA4A022BAE673F" modified="2017-01-18" version="1">MDDLSETDETKLDTGATEDSLDVDATLDSLFWYVGTNDEEKEVSELDETLEEEADEPTDEAAEDSLEAKLEAEDSLEADVAIDEAAEDSLEAKLEAEDSLEADVAIDEAAEDSLEAKLEAEDSLEADEAIDEAAEDSLEAKLEADEAIDEAAEDSLEDSLEAKLEAEDSLEADEAIEEATDDSLEAKLEAEDSLEADEAIDEAAEDSLEAKLEAEDSLEADEAIDEAAEDSLEADEAIEEATDDSLEAKLEAEDSLEADEAIEEATDDSLEAKLEAEDSLESDEAAEDSLEAKLEAEDSLEADEPIEEATEDSLDELMIDDGPTWDLLKDEVEEELEIMDGSVAEGPSEIVVVSEQSIKEAKPEQSNSRALENTGQSPNDTTPQTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUD2</accession>
    <name>A0A1E3NUD2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Actin cytoskeleton-regulatory complex protein PAN1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Actin cytoskeleton-regulatory complex protein pan1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">WICANDRAFT_81571</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Actin patch</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PAN1 family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ56736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035943.1">
        <property type="nucleotide sequence ID" value="XM_019185260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUD2"/>
    <dbReference type="STRING" id="683960.A0A1E3NUD2"/>
    <dbReference type="GeneID" id="30202506"/>
    <dbReference type="OrthoDB" id="2015333at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0006897">
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Actin cytoskeleton-regulatory complex protein PAN1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="DUF1720"/>
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    <dbReference type="PANTHER" id="PTHR11216:SF173">
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        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF1720"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="2"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
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    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="92"/>
            <end position="168"/>
        </location>
    </feature>
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        <location>
            <begin position="123"/>
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            <begin position="593"/>
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        <location>
            <begin position="594"/>
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        <location>
            <begin position="1"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="17">
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            <begin position="213"/>
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        </location>
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            <begin position="984"/>
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            <begin position="246"/>
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        <location>
            <begin position="999"/>
            <end position="1019"/>
        </location>
    </feature>
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        <location>
            <begin position="1023"/>
            <end position="1044"/>
        </location>
    </feature>
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        <location>
            <begin position="1049"/>
            <end position="1065"/>
        </location>
    </feature>
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            <begin position="1066"/>
            <end position="1084"/>
        </location>
    </feature>
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        <location>
            <begin position="1107"/>
            <end position="1126"/>
        </location>
    </feature>
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        <location>
            <begin position="1128"/>
            <end position="1143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00009351"/>
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            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Proteomes" id="UP000094112"/>
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</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUK2</accession>
    <name>A0A1E3NUK2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear speckle splicing regulatory protein 1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_65688</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
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    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NSRP1 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183968.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Nsrp1"/>
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        <property type="entry name" value="NSRP1_N"/>
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        <property type="entry name" value="NUCLEAR SPECKLE SPLICING REGULATORY PROTEIN 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NSRP1_N"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="39"/>
            <end position="145"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
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            <begin position="132"/>
            <end position="172"/>
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            <begin position="9"/>
            <end position="18"/>
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    </feature>
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        <location>
            <begin position="140"/>
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    </feature>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF09745"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ56808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="218" mass="25773" checksum="174F243DCABCCF82" modified="2017-01-18" version="1">MSRKALGFDDSESEDEAEVVAPKRPGTHRDLIAESLKNQAKVKKYEESTPDIYQYDEVYDDIEAERESKKPKKQDDGGSKYIERLMEAKKQRNMDKLYVQDLKVQKERELEGDEFKDKESFVTSGYKEFREERMKEQENENMGNKSQGNFRESILKKNEPLKSSPNVSKPKRIITHELAKPQFVEEPVTKSRYLPKLTTDEIEQYRMRYFERKKTAII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUL6</accession>
    <name>A0A1E3NUL6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_65657</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035981.1">
        <property type="nucleotide sequence ID" value="XM_019183960.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3NUL6"/>
    <dbReference type="GeneID" id="30201206"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000065">
        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002058">
        <property type="entry name" value="YML081W-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF13">
        <property type="entry name" value="RESPIRATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="759"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="804"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="194"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="97"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="255"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="322"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="528"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="635"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="334"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="533"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="587"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="635"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ56774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1303" mass="147213" checksum="7134B58192E4B785" modified="2017-01-18" version="1">MALRNYMYYKHEDELKDKIAHGLNVTLNVNTTTSTPNTSPVIPRKPKRLTPTQIRKLERLKLEQQNESDNDANSSCDNLSSAVETAVVLMLGMPTPPSVASSISPLNNNDSLGSVSASQSPPIGDNNSNSGSRKRSSLKQYQQSVQGFEPIPKKSAIIKTDKPRPHVCTTCTRAFARLEHLKRHERSHTNEKPFQCAACGRCFARRDLVLRHQQKLHTSLTTNNTNSKDFKKGENENIITNYGNKEVSLPTKNGVVIDEDPRSNTKRESVSSVGSDHHQHQLIPPTLKSFNLNNNFPTKSTIESYNEHSTLFKHAENLRHSSISHDNSDTASHTTHHSAHPPQKRHRHNSFSASSAISYTKNDQEAIHNEHELPEAPHQVGFATPQLTAQELTSKAIMSGFDLNALGLQYEIDDLNLDINGQPSTGQTTGVANTPVAVAAASGSSQQQRSFQQLHEQQDYFHSQNGSRAVGSAAHTQNPSRVSTPYQFNFTPGGNLIDMPILNEYLHLGGAGGGAGFMPKNPNVNMNLFNHNSVAPSPSQQHSQVHHSDDHTTNADAGSSSIIQSHPLQHHQHSHPQSGGTVHDDTTNTSNSIHDNSSTPDDWLCEFINTPFDQNFTMSSNAKNFNSIGFVPSVSPSNSTGSAPQIENPLPQQQLQKQQKQKIDPNQLFNNDIPSLFRSRQIDLFKKEIENNEEIKSSVHLFNKQLRDEILTLNKLNDSQFPSLDELNQYFTLFKNEFNKYFPFIHLPTLQASTDNYPLLLSIASIGALYGFHSTHAMLLFNISRFRIHEFLEHEKYFKNNNNIPIWVYQSMVLLIFIGIFNNDLSITKTMTIMLNSLIELVKLTSLNLPLENFLTPPPIINNKSSGGYDDVLEQQNFNYFILAQTRIRTCHTILLISNLFTSLIGLDCSLHSIDVKCGIHCKLESLWECADHKEWNEILHREHYVIDSKFALIELSNGNDSYNHCLNYLSMNNYILNEDDQLNKKFQMKTLLSLLMSIHEKVYLERTTLKDEKNGVIKATKWRMNSRPVLESLIKSWESLYIKNGGVLYLQDSNLSLIYNKDPVLRLILPLLSFVKIRKCIHLTPIIEKIWLKDWDGMNECLKQLSDDPEALKDATNYALDIVKLWIDYVSIVNDAEKTSIRTPIFFIICMFTSLLIIAEFLLSVEKWAHNFDEENPTTYLNAIERTLWLRSEMIFKKVESNLILNENNKTYAEFLRLEAKGALDVKTLDDELAKRAMNPNTEIKETLQVIKDARLSSRGLYMGVRILADAPIWPIALLFAQALKSRAIYISKSQPSSPSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV42</accession>
    <name>A0A1E3NV42_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MINDY deubiquitinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_18769</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036276.1">
        <property type="nucleotide sequence ID" value="XM_019180806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV42"/>
    <dbReference type="STRING" id="683960.A0A1E3NV42"/>
    <dbReference type="GeneID" id="30198052"/>
    <dbReference type="OrthoDB" id="10261212at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016807">
        <property type="term" value="F:cysteine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990380">
        <property type="term" value="F:K48-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007518">
        <property type="entry name" value="MINDY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033979">
        <property type="entry name" value="MINDY_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18063">
        <property type="entry name" value="NF-E2 INDUCIBLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18063:SF6">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04424">
        <property type="entry name" value="MINDY_DUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MINDY deubiquitinase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="307" mass="34547" checksum="10F32E06320095AD" modified="2017-01-18" version="1" fragment="single">EIVFKTKTIDFDGRRNILIQNENGPCALLALVNYILLTVDHLSAPEIHDLVALVNSSNEIPLSGLLNYLQQIVTANQRTYGIPPEEVQTTLSFLPSLHEGLNINPQFDGTFSPSHELALFRDFNVPLVHGWLVDPSAPEYRGVIKYNSYETAQNVLVEAYDVEQKANKDSVDLEIIDDSRQIKSFFARTATQLTDYGLHYLRDNLQNNSIAVLFRNDHFATIIKKDNDIYALVTDVGFATKKSFVWESLLSINGANNSFFTGNFSPILEDSSNPFDDSNVDNDDLNSAEERDRALAIAMQEEEDNRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVA5</accession>
    <name>A0A1E3NVA5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Required for respiratory growth protein 9, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_19185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ57056.1"/>
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    <dbReference type="Proteomes" id="UP000094112">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_mL58"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR28266:SF1">
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
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            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="15773" checksum="3EE1859A111D84E4" modified="2017-01-18" version="1" fragment="single">LIHHPPPSAPSSTSEIPDLFLPNDDPRKGLNLSGEQIDINNAPPLSTPSEKKYHLSPKDVEEIQRLRASEPYTYTKKVLAEKFNVSPFTISLVSDVSKERKQDMDDRLAQIKQGWSKGKAQARLDRKKRQQYWYRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVC4</accession>
    <name>A0A1E3NVC4_WICAA</name>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycetes</taxon>
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            <taxon>Wickerhamomyces</taxon>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ56980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="30201122"/>
    <dbReference type="OrthoDB" id="3980661at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF10224">
        <property type="entry name" value="DUF2205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ56980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="139" mass="15224" checksum="0889AD86C442FF3D" modified="2017-01-18" version="1">MAATTPESSIEKFSDTLNNNQETIPSHETGGQSIPSAIQDESTATQPQSGNDDSIVAQQATIPETTITAPAAAATTSDEEMKPFSKYEQAVQLRDKLDSLIRRTVQTKKKCDKLDHDNKYLQEYVGNLMNSGDFLSKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVP8</accession>
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            <fullName evidence="3">PH domain-containing protein</fullName>
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    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ57241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019186000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVP8"/>
    <dbReference type="STRING" id="683960.A0A1E3NVP8"/>
    <dbReference type="GeneID" id="30203246"/>
    <dbReference type="OrthoDB" id="2264563at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046868">
        <property type="entry name" value="BAR_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046869">
        <property type="entry name" value="SLM1/RGC1-like_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941:SF15">
        <property type="entry name" value="ACTIVATOR OF SKN7 PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941">
        <property type="entry name" value="CYTOSKELETAL SIGNALING PROTEIN SLM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20400">
        <property type="entry name" value="BAR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="339"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="498"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="593"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="523"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="599"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="659" mass="76147" checksum="92D196B8E0EB763F" modified="2017-01-18" version="1">MSYYHYIDDETILPKTNYNSPFYSSVPLHSQPTDILSKRFELWRQIIKSLINYFKSIAIANQQYSKINDNLIQTINFPFFTNIFPKVGGVAMENNKIFEPHVEDLKKQTYFASFGNGSIQDIQILLKKYHLNLGQQQRTISIELINTLVPRLEELQKDLFIKIREIKNLNSDFKNSLNQEIAITGQCLNDYIDVVKMYKNGVIESGSGKKDPYLLRLKLEIQLKQQLYQENYLEEAFINLQITGLELEKIIFQEIQNSLNRYNELISQEIFVMYNDLINELHQGIISKKNFFEWDESIKKDSSAEFKKHFLTILHDDQLPIPRKLSSIKYPYNKSIISKCFRSGYLLKKSKLLKNYSKSFFILTINYLHEFKSSNLMESPTPINSINLNDAILTESNDMKFQLHVKHIIGPDNKIKTHNYVFKRSLNEMDEYQFKKWIIDLKNMTSFNSFNERFKYINGKLSNSNSRSNTPMFEISNPMDNIDKVNEKLQNLQITSITNEANEGREGEEQQEQEQEPEPQAPSPSQLSPSPNEQQDIPQNFTQFAQNIKPTLPKLSLSSLPKVQIQEPSPIHPSTTTSTSSEDLIKLAIQHNQQARPASITSSTNTSSLGSSSISAIGVPTLTKQKSHIDLNGSLYKDEDKKEEDDELNKVLDALKNEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVU1</accession>
    <name>A0A1E3NVU1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SIS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_65585</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036536.1">
        <property type="nucleotide sequence ID" value="XM_019183943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVU1"/>
    <dbReference type="STRING" id="683960.A0A1E3NVU1"/>
    <dbReference type="GeneID" id="30201189"/>
    <dbReference type="OrthoDB" id="1872003at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05014">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="SIS_Kpsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38418">
        <property type="entry name" value="SUGAR ISOMERASE, KPSF/GUTQ (AFU_ORTHOLOGUE AFUA_6G08860)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUGAR ISOMERASE, KPSF_GUTQ (AFU_ORTHOLOGUE AFUA_6G08860)"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SIS domain"/>
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    </dbReference>
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        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="55"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="378" mass="41732" checksum="FE368356A4872C87" modified="2017-01-18" version="1">MTKQPAIHHDSHVQKSLETFGSILQNQTSAIHHLVNQYKHSQFSQNQLKKSLNILNTALLKNGKIVISGIGKSYKIAAKTVATMNSLGMHSALLHPSEALHGDLGILRKENNDCLIIISSSGKSPEILQLLPHISYEIPIILLTNKKNSILSSHPQVKSLLYAELPSQLTEQSIYGLTAPTISTTLCLTLADAVSIALAELHVADLSERKRIFGERHPGGAIGEAYGNVLSSHNSSMLNTPSYSSSSLSSGTGSNSNFSPALNPTNSISSDDDEPKLEDLPKVVFDIGYENDVQGQELIRAAKESKNVGQISGFFKSEEEILQSIVLYDFVVFSQSFVIASDRLKEVYKLVHEHQYENSTDKFEELKWRIKENLSRYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVU2</accession>
    <name>A0A1E3NVU2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_15349</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036442.1">
        <property type="nucleotide sequence ID" value="XM_019180642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVU2"/>
    <dbReference type="STRING" id="683960.A0A1E3NVU2"/>
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    <dbReference type="OrthoDB" id="2011986at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="UPF0662"/>
    </dbReference>
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        <property type="entry name" value="CU(2+) SUPPRESSING AND BLEOMYCIN SENSITIVE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UPF0662 PROTEIN YPL260W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10303">
        <property type="entry name" value="DUF2408"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="431"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="153"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="432"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="459" mass="53299" checksum="80B3793F9398B35A" modified="2017-01-18" version="1" fragment="single">HTNEIPKNEQDIFNQLTEIRTKLSILKKDRSQYLNSKDVLSIYTNVLTLVKNLNNIRHQVEDRISNRVDQVVDDVFQLLSLFFITVGLSKTAPATYASLSTVQRLLEHLNESRVYTHHDLKPIKERLSEIEEIIEENKNPVDEEEDFLLKRKLKECQLEYNEVENKVEDVDNSLNSLLEELITTRRKLLSIAARPDFKTQELTPLKLKLQELGKHRDLNGKFTSLETGKFEENGQSVLNGLYDDCNILIKDLEAHKFELDDNLKPIYDRLINLKTTLEDLLVTRRWTLRETDLYTYQKNLQELDDLRVDGKFPTKDESKSNKGQSILLYLLRRCYAIIYKLLESSEPVSEALQPIHNQLSTVRRCLMEVRRMGGINSARELYPYQMKLASLDNLREDGKFIVDGQIPEGQGTLNALLAECYDICQEMKVEAEENETEEQGVSDDVDDGTNLYDHDDEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWC0</accession>
    <name>A0A1E3NWC0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_106934</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
            <location evidence="1">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWC0"/>
    <dbReference type="STRING" id="683960.A0A1E3NWC0"/>
    <dbReference type="GeneID" id="30197733"/>
    <dbReference type="OrthoDB" id="248495at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051754">
        <property type="term" value="P:meiotic sister chromatid cohesion, centromeric"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13981">
        <property type="entry name" value="STKc_Bub1_BubR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.430:FF:000003">
        <property type="entry name" value="Checkpoint serine/threonine-protein kinase BUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015661">
        <property type="entry name" value="Bub1/Mad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013212">
        <property type="entry name" value="Mad3/Bub1_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012572">
        <property type="entry name" value="Mad3/Bub1_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14030:SF4">
        <property type="entry name" value="BUB1 KINASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14030">
        <property type="entry name" value="MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08311">
        <property type="entry name" value="Mad3_BUB1_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08171">
        <property type="entry name" value="Mad3_BUB1_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00777">
        <property type="entry name" value="Mad3_BUB1_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51489">
        <property type="entry name" value="BUB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0137">Centromere</keyword>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BUB1 N-terminal" evidence="6">
        <location>
            <begin position="50"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="540"/>
            <end position="833"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="833" mass="95849" checksum="3698DB12399CD7D0" modified="2017-01-18" version="1">MEIIDFESIEQQKENIEPLNEGRSAAALKGLFSVPLGDLKKQQLDERSKIEAELESLEELDDPIQPYLDYIQWIKTNYPTGKSVESGLIQVLERCTSQFRDYDFYKNDPRYLKVWLTYAKYSDNPRDIFVYLARKEIGTSLALYYEEYANFLETNGRFAQAEQVYQEGLSVKARPLARFQRRFDEFKIRFQHRPVSENIEPSSPVFPVRSALSLKSGTLFASHDTARPQKKRLEIFNDTEADNGIRQDGWDILGSNKFRNKENILEAKPWAGEVLRQDNVKKTNCNKVSVFQDGNDYPSGPVYKIIEIPGKKPEKIDLNFDLIYQEGEELCIQEVLARLRGLNKQNETNFSTALSTPVMTSLIDEKKATTVDSTIHLNKNDLKRPSSPTATFFTNAAKNEVYSMFNQNPVKEKQSQKEEDEEELIYDDYTEQLTRPNMNDLTERMVDVGLQQNDDLNNTEGRNDTEISNDAHAIDFPTVDPADESLKAALLKEIMPPLSSYFGLFEYNETLEMCSTLKKSLNNKLSKHVFLNLKGSESTFNLRSLLGEGGFASVYLAESMNGQLKAIKAQKPSSAWEFYILKQIEQRLKGKDILDSIIMSEEIHLFRDESYLVLEYEKQGTILDIVNLYRSTGRNVDEVLAIFLTIELLKVIEAIHNIGIMHGDLKPDNCMLRLENCEVGTYKRNGGNSWSKKGIKLIDFGRSIDMSLFPPNIRFKSHWKTDNQDCPEMRNDQLWTYQADYYGVAGIIHSMLYGSFIETVLVNGKYKISNPMKRYWHHELWNPLFDILINSADYGNLPITNKLKEERERLESWLEVNGSSLNGIIRDIETGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX01</accession>
    <name>A0A1E3NX01_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_35367</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036870.1">
        <property type="nucleotide sequence ID" value="XM_019182295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX01"/>
    <dbReference type="GeneID" id="30199541"/>
    <dbReference type="OrthoDB" id="2797145at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="68" mass="7336" checksum="73788B6A13B2B637" modified="2017-01-18" version="1" fragment="single">RTVPGNFGAIFWGFMEMFNFGTSKSLSAGMAPANNLQNVVLPVPFSPIITKISESLKSPFSICNLKSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX44</accession>
    <name>A0A1E3NX44_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ADP-ribosylation factor-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_34862</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036747.1">
        <property type="nucleotide sequence ID" value="XM_019182245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX44"/>
    <dbReference type="STRING" id="683960.A0A1E3NX44"/>
    <dbReference type="GeneID" id="30199491"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031503">
        <property type="term" value="P:protein-containing complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04151">
        <property type="entry name" value="Arl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000510">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="25"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="127"/>
            <end position="130"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ57540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="20192" checksum="301F2A406A028628" modified="2017-01-18" version="1">MGNVFSGIFGKLWGVNKEIRILILGLDGAGKTTILYKLQIGEVINTKPTIGFNVETLSYKNLKLNVWDLGGQTSIRPYWRCYYANTAAVIFVVDSTDKERIEIASKELHTMLSEEELADSALLVFANKQDQPGALSAAEVSKALNLVELKDRSWSIVASSAIKGEGLTEGLDWLIDVIKDEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXJ6</accession>
    <name>A0A1E3NXJ6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">VLRF1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_17169</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The VLRF1 domain mediates binding to the 60S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ANKZF1/VMS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037118.1">
        <property type="nucleotide sequence ID" value="XM_019180729.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXJ6"/>
    <dbReference type="STRING" id="683960.A0A1E3NXJ6"/>
    <dbReference type="GeneID" id="30197975"/>
    <dbReference type="OrthoDB" id="429841at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047139">
        <property type="entry name" value="ANKZ1/VMS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041175">
        <property type="entry name" value="VLRF1/Vms1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16036">
        <property type="entry name" value="ANKYRIN REPEAT AND ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16036:SF2">
        <property type="entry name" value="TRNA ENDONUCLEASE ANKZF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18826">
        <property type="entry name" value="bVLRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52044">
        <property type="entry name" value="VLRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="VLRF1" evidence="12">
        <location>
            <begin position="199"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="369"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="519"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="377"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ57911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="600" mass="68620" checksum="36FE7C8509E0FC0C" modified="2017-01-18" version="1" fragment="single">FQQDETLVYDLSDRVLRSLQLMHFDSSTTEEVLPQQKLTSEALSHIDSQSSNKNQHEVKDKDYYKSDLHRFNLKRDLKGLPRLTEEEFEELAGDLDESISGSDSSDSEEEGEGERKDIYAYKKDKLDTIFEKNIKQLEDLKLQESDRVVSHLATRSPYILFKSDLLDQDQCFGVYKSLFSIKELEEPIEALKSWKSKSSSGKSALLMIGGGHFAGAIINHKPKSTKGNAIKPGESILEQGVDIVAHKSFHRYTTRRKQGGAQSASDNARGKANSAGSSLRRYNEVALQNEVRELLQSWKKELDECDSIFIRANGSSNRNIIVGYERAVLRNDDERIRSFPFTTKRATTSELKRAWVNLTYLNITSRPKVDEKKLKQKQQQEQIQQSRIQKEKELKEISPEEKHTAELVGYLKKSRGPVLITYLKKHKLSPNFQLKPESEYFNTPTLLHFAAAHGVKNLISILIKTAKADPTITNNNGKTPFDLSANKQTRQSFQITRFDLGEDSIDWEKAHVGKAISREDVEKQEADEKKKEEENKQKLIKEELTKKEENIAASKENKRLGGVNLSEANLNSLSDEQKMRLMREQRARAAEARFKQQQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXP0</accession>
    <name>A0A1E3NXP0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="5">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_80041</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037081.1">
        <property type="nucleotide sequence ID" value="XM_019184992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXP0"/>
    <dbReference type="STRING" id="683960.A0A1E3NXP0"/>
    <dbReference type="ESTHER" id="hanan-h6uwn2">
        <property type="family name" value="Fungal_carboxylesterase_lipase"/>
    </dbReference>
    <dbReference type="GeneID" id="30202238"/>
    <dbReference type="OrthoDB" id="6846267at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009027329" description="Carboxylic ester hydrolase" evidence="5">
        <location>
            <begin position="18"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="6">
        <location>
            <begin position="31"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="573" mass="63271" checksum="AC5A03F9BDA24127" modified="2017-01-18" version="1">MLLKSFALIAISTLASSAPIFSKRADDQAHEPIVQVLNGTLQGSYLSNFHQDAFLGIPFAEPPLDELRFQPPQTIKHKYNEQLKVVNQYGHSCMSTSVSADNITQSEDCLTLNVIRPSGYENQSLPVAIWIYGGGFYEGSTAMPAYNLSYIVQNGVEIDKPFIGVSINYRLTGFGFLASQQVVNKGYSNVGLRDQLKAIQWIHENIAMFGGDPDHLVIWGESAGALSVSKLLVSGHLQDYVKGAIIESGSVLFPNTTSGAVEYHQDDYDTLVDHFNCSQASDSLECLQKINVTDLLEVFNLSNNVLTTGFIYPYIDGDILTKASYNTLLDGEFNKVPLLIGTNTDEGSLFIGSGVNTTEEFEYYMKMALPSLTNKSIATLNELYPVNDSSINVPLDPTYNSSAIVYPDSLGKQFPRLATVFGDLAFIAGTRIMSRVYANNSVPVYKYRFNIPDLALSLAPYYGAGHAQELVYVFDNNKALTNTSEAISAGFNPSPKSANISESMSKMWANFITELDPNVNHDKVPMEDVPEWPEYGDEAKNMVFDLNGFYLEDDDFRSKQIDFIESIMGQLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXY0</accession>
    <name>A0A1E3NXY0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_80120</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037162.1">
        <property type="nucleotide sequence ID" value="XM_019185009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXY0"/>
    <dbReference type="STRING" id="683960.A0A1E3NXY0"/>
    <dbReference type="GeneID" id="30202255"/>
    <dbReference type="OrthoDB" id="306876at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="47"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="183"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="362" mass="40820" checksum="4DAAE4F922BBE96E" modified="2017-01-18" version="1">MSKVKEFYKNHIQRDIGLYSVIGSEFCNSWMLVSCKLLETSDPDSPIHPFQILFVRMVITYAACILYVLLRNVENAPFGPKEVRWALVLRGFFGFFGVIGLYYPLMYLSVSDVIVIQFLAPTATALIAYLFLREYFTKLEALSGLISLGGVLLIAKPTFLFGDSANSYIDDKVESSDPTQRLIAILVSLVGVVSGASSLVTIRHIGDRANTVIMISYFALITAILSFFTIILTPSLSFKMPGSASQWGLLLLIGVSGFFMQFLMTFGVQYEKASRAASIMYVQIIFGIAWEFLIWHHLPNILSWLGIIIILSTSFCVFYFKKREDDDIPHRVLDEEEDFDSSVIESHEEFDIPMKKLHKVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYE6</accession>
    <name>A0A1E3NYE6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_95417</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037291.1">
        <property type="nucleotide sequence ID" value="XM_019186491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYE6"/>
    <dbReference type="GeneID" id="30203737"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="70" mass="8056" checksum="A4B7F822B6997B3F" modified="2017-01-18" version="1">MINFLLALFRLELDRLDEGLLVLFRGTSFYKSITYLLSLLDHWFCAGLNGSTTITSTSSFDKIKQKHLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYM8</accession>
    <name>A0A1E3NYM8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_95589</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037381.1">
        <property type="nucleotide sequence ID" value="XM_019186498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYM8"/>
    <dbReference type="STRING" id="683960.A0A1E3NYM8"/>
    <dbReference type="GeneID" id="30203744"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004491">
        <property type="term" value="F:methylmalonate-semialdehyde dehydrogenase (acylating, NAD) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006210">
        <property type="term" value="P:thymine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000002">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase (Acylating)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010061">
        <property type="entry name" value="MeMal-semiAld_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866">
        <property type="entry name" value="MALONATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866:SF3">
        <property type="entry name" value="METHYLMALONATE-SEMIALDEHYDE DEHYDROGENASE [ACYLATING], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="333" mass="35621" checksum="C2FE20350E00F4D1" modified="2017-01-18" version="1">MIPLWTIPYIIATGNTTVLKPSEMCPGTGAILAEIAAEAGVPEGVINIIHGKAPTVNKIVSDPRIAAVTFVGGNNAGKYVHSEATKNGKRAQVNLGAKNHVVVLPDADKDTVLKAVVTGAFSSTGQVCLSTDILFLVGEARGLIPDIVKEVEKLQAKPGFQDGDFGPVVTKESLQRLEAVIQDAVDKGAEVLVDGRGKKPQGFEDGYFIGPTLLRNVKKGMRCVDEELFGPIFTIAEAETLDETIDYCNNNEFGNSVAVFTNSGPDANKFAKQINIGQVGINVPIPIPLAQFGFTSNKSSFLGDLHFYGPTAFKFLTQPKTITTSWKNIKTKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYP0</accession>
    <name>A0A1E3NYP0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_64254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037317.1">
        <property type="nucleotide sequence ID" value="XM_019183698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYP0"/>
    <dbReference type="STRING" id="683960.A0A1E3NYP0"/>
    <dbReference type="GeneID" id="30200944"/>
    <dbReference type="OrthoDB" id="4067208at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072380">
        <property type="term" value="C:TRC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000753">
        <property type="term" value="P:cell morphogenesis involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.70">
        <property type="entry name" value="Get5 dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040474">
        <property type="entry name" value="MDY2_C"/>
    </dbReference>
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        <property type="entry name" value="Mdy2_get4-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16843">
        <property type="entry name" value="Get5_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18514">
        <property type="entry name" value="MDY2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="70"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="206" mass="22670" checksum="596D77DAE14F8C15" modified="2017-01-18" version="1">MSVATSATQSEREFASNYLTLLSVSDSKIVIPADYRKDLKDISTLGIKLPKLPVSKKQVNGDSNDSDQIIDVTFKSIKPPRFNTSFKSSTSNTLFEVKTLLVKNEGNLQGITQSQIKLLVKGKVIQDSVLLGSVANCEEHVTFTVMINKDKTSDSTPVSTPEPSTITPITKEIELPWDEIKVLLQEKGIDASSAITRLQKGWELTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYU4</accession>
    <name>A0A1E3NYU4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_65102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036964.1">
        <property type="nucleotide sequence ID" value="XM_019183857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYU4"/>
    <dbReference type="GeneID" id="30201103"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="108" mass="11253" checksum="4A174441BC65185F" modified="2017-01-18" version="1" fragment="single">MDKLSWSNFIWYCFRYPTTGTDGVVGTGTTTIPFPGASTSHSWLVTTSTWTNSAGLTSSGIVSEYPTTGTDGVVGTGTTTIPFPGASTSHSWLVTTSTWTNSAGLTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ13</accession>
    <name>A0A1E3NZ13_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Agglutinin-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_80106</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037149.1">
        <property type="nucleotide sequence ID" value="XM_019185005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ13"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ13"/>
    <dbReference type="GeneID" id="30202251"/>
    <dbReference type="OrthoDB" id="3981162at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2430">
        <property type="entry name" value="Agglutinin-like protein, N-terminal domain, N2 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024672">
        <property type="entry name" value="Agglutinin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043063">
        <property type="entry name" value="Agglutinin-like_N_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033504">
        <property type="entry name" value="ALS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011252">
        <property type="entry name" value="Fibrogen-bd_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793:SF2">
        <property type="entry name" value="AGGLUTININ-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793">
        <property type="entry name" value="ALPHA-AGGLUTININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11766">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01056">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="Bacterial adhesins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133578" description="Agglutinin-like protein N-terminal domain-containing protein" evidence="13">
        <location>
            <begin position="24"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Agglutinin-like protein N-terminal" evidence="14">
        <location>
            <begin position="54"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ57942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="341" mass="37266" checksum="E4368977DCB3B64F" modified="2017-01-18" version="1">MSLSSRFLGLWVLFSIFVHQVYAKQVNGIFTSINSIQLYQGNGYPFDFYKVNVGWKITQSQNLQTGDTFTLNMPNVFEVRLTQNGGFGNYFDIVLDNGQHIASCTFDQAGGRASSTSVNCQLTADISNYDTLNGDLDFDIIFDGGGRIETTSAASRWTSGTNTITFNNNLSKTVSFNTPNTDPAQFISRYTMKGDVFYYYLAPKNLCYGAGVRSGTFTFSVGSGPGDFGKLDQSLTETYSTGNISPFGYPKSYSALGGANPSYSSNGRTFTTTFNSINSGERFWFSGFSKYTYISGTYAVSYDLRVNCNNGWTDESANTINLQIVQGNAGSGGDGTGKFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ70</accession>
    <name>A0A1E3NZ70_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA damage-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_57231</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DDB1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RSE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037602.1">
        <property type="nucleotide sequence ID" value="XM_019182795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ70"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ70"/>
    <dbReference type="GeneID" id="30200041"/>
    <dbReference type="OrthoDB" id="436637at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001143">
        <property type="entry name" value="Pre-mRNA-splicing factor rse-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000031">
        <property type="entry name" value="Splicing factor 3b subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="10">
        <location>
            <begin position="23"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="11">
        <location>
            <begin position="433"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="9">
        <location>
            <begin position="782"/>
            <end position="1087"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ58395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1120" mass="124383" checksum="350088A48C1B8D85" modified="2017-01-18" version="1">MSLDASICLYNLTLQKQSASINSCVGNFSGKKSQEIITATSNSLTVYKPDVDTGKLVNVLTTQIFGIIRSVANFKIAGAGKDYLAVTSDSGNLSILELDLPNGQFNALFNEPYHKSGIRRLSPGQELAVDPKGRAILATAVEKNKLCYVLNRDLENNLTISSPLEANRSKILTINTVGLDVGYENPLFATIEIDYSDYEYNEVTEYEKYLTYYELDLGLNHVVRKNSEKISPTSNLLLQVPGGNDGPSGVLICSQNMISYRGLEGEKVSINIPKLKNDQANVFIVSGVLHKMKNQFFFIVQSNNGDLFKVELLEDGSIAISYFDTIPLTTSLIILRSGFMYADTQYSSKAFYQFEKLGNENPFTSSSDEPIEELTFERTEELENLLLVDIQESLNPILDSKLVSDDAFTKIYAASGYKDSAQLKVLQYGLPINELVESDLPGVANKVWTTKLHKADQFDRYLVISFTDTTLVLSIGESVEEITDSGLALNEETIGVQQIGAHSIAQVHSNGVRNIKNGQVVSEWFPPAGIKIIVSSSTNRQIAVGLSNNELVYFEVDERDRLIEYGERKELHTRTVSLSLGDISEGRLRSPFLVVGCDDSTIRVLSVDPKSTLESLSLQALSSAPNDILTCTMNGQLFVNIGLENGVYVRTLLDERTGQLSDTRTKYLGNKPVVLSKVVVSGINVVLALSNKTWVVHETKNGAFKINSLLINPLKFGFIFNSEECTDGIVGIYKRNLIIFTIDDLVHDFNITSSELKTTPKNLLRYKESTIVAQNSSEASFIEKFDESLELVKSEQLAPNERIITSGVVHFQSKDASYVVVSVSKTVESSTEYYINTYDIDLNFKHATKIADAAYSIVEFQGKVLIGVGNHLRLVDIGLKQLLSKANSRIESVSQIVRIESQGNRVVIGDIRESVTFLIFKPNMNEFLPFADDVLPRHITSIKLLDYNTVIGGDKFGNLFVLRATEEASKVSDTNSTFLITQDKVLNGAPYKLSNICNFYIEDIPTSFSKGSFTIGGRDIVIYSGLQGTIGALIPLLTKSDIKFFHNLEKAMRTYKEDLLGRMNLMFRGYYQPVKNVIDGDLIETFNTLSDDLKIQIANELDKTPREVSKKIYEVRTMSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZG8</accession>
    <name>A0A1E3NZG8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_80534</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037597.1">
        <property type="nucleotide sequence ID" value="XM_019185081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZG8"/>
    <dbReference type="STRING" id="683960.A0A1E3NZG8"/>
    <dbReference type="GeneID" id="30202327"/>
    <dbReference type="OrthoDB" id="444255at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="6">
        <location>
            <begin position="423"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="875"/>
            <end position="932"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="956" mass="111457" checksum="C1EF8B8F6B035856" modified="2017-01-18" version="1">MLFRKTRLLVILSVIILANLGLLSILNYDKERILALTSLKSVLRTSTDLKNEDLDFEFVKNNFLGTEIVSKYYIPDYLYNSKSKTYNHDPRLTSGIYLNHITSEFKKHNDLANVKVPFSWYDWTDLSKDLNKLINLPQKFKPDCSYVCTNYFNKQLQKELEEKIKGTFEEKEAIDPNNDGKVNKNGRKRKSTRYQMQKYSYCRDDNSENQPGFRIFKNHLKSRPDVKALQSKSYLYSEASAPVSLVFLTKNGNLQIEVLTNEESENKQDYGLLKNKMIQNYADSFRANNPKEEVYVDSRIELGELFETIGNIDPVSTPLSLKTYQLDLNHSQFEFDGPQYLNSSPTSSLSKHQTNFQNSIKNSLQTKELLVPKYFEEVLLSRTRASLQGGHFDWRFFSGDVPLDQRQSILSRLLQNWFKLTTNAGLHSWIAHGNLLAYSFNGLNFPWDDDIDVQMPFNDLAKFAERYNGSLIVEDPEEGFGRYFIDVTSSLTHRLKGNGNNHIDARFIDVDTGLFIDITGLAVSASDTSQRFQNRYEKMIRKGEAVEYSDPNYYEGIYEDISRDDMRKLAKENLVLYKQLRQFERENSRSRGNLLKDKSSEERYDINYKLQTYNCRNDHFVSFDELSPLKRSFFEKIPIFVPNNVSAILLDEYKPQRKLSAEHSGFCYLPQIRLWVPRKTVANIVRNEDKMANLNLLTQNDIEKLLEDESIFAEYFKTFDATLVREKEKEIINDESIEQKDQLLSKLVLGKRFPSLRKDVHMLRLENKVAEKFNIKFSKEDISDQIISQLRSEYSKTVDYNILGAGSPQDINFNNYGQLYKDMLKKAQDENYEPDFEMKKEELQQALYRARNNIEREQKQKLLDQLLDTEIKEKFDSNIPKPPLLQKGQVQGVERPNNRPMDEEDDPGVVNDAQNDEKSPMAQEVNQLPRLDVEVDEQKEAEKLKQFEAIHGEMIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZI0</accession>
    <name>A0A1E3NZI0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="8">ATase</shortName>
            <ecNumber evidence="3 8">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_84367</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="3 8"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037780.1">
        <property type="nucleotide sequence ID" value="XM_019185700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZI0"/>
    <dbReference type="STRING" id="683960.A0A1E3NZI0"/>
    <dbReference type="GeneID" id="30202946"/>
    <dbReference type="OrthoDB" id="191723at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11907">
        <property type="entry name" value="AMIDOPHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="11">
        <location>
            <begin position="2"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ58573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="416" mass="46167" checksum="1BA92ECF97D67B77" modified="2017-01-18" version="1">MCGILGLCLADKTANAAPELFEGSLFLQHRGQDAAGITTSGPRGRFFQCKGNGMARDVFTPQRLSGLVGNFGIAHLRYPTAGSSAGSEAQPFYVNSPYGISLSHNGNLVNTLQLRKYLDESVHRHINTDSDSELLLNVFASELDKYNKARVNNEDIFNALKGVMDMCRGAYACSGLLAGYGLFAFRDPNGIRPLLFGERVNVNGKTDYMVASESVVLKAHNFKKFRDILPGEAVIVQKSGHVEFRQLVPIQNYTPDIFEYVYFARPDSVLDGISVYHTRLAMGAKLAENIKGIFEGKDISKEIDVVIPVPDTSRNSALECAVTLNLPFREGFVKNTYVGRTFIMPDQKKRVSSVRRKLNAMESEFKDRNVLLVDDSIVRGTTSKEIIQMAREAGAKKVYFASAALNLNQYHRYDYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZV8</accession>
    <name>A0A1E3NZV8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_79165</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037825.1">
        <property type="nucleotide sequence ID" value="XM_019184845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZV8"/>
    <dbReference type="STRING" id="683960.A0A1E3NZV8"/>
    <dbReference type="GeneID" id="30202091"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008079">
        <property type="term" value="F:translation termination factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006449">
        <property type="term" value="P:regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18626">
        <property type="entry name" value="CD_eEF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000076">
        <property type="entry name" value="Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.990:FF:000002">
        <property type="entry name" value="mRNA export factor elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000193">
        <property type="entry name" value="Probable Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015688">
        <property type="entry name" value="eEF3_ABC2_chromodomain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047038">
        <property type="entry name" value="eEF3_chromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056891">
        <property type="entry name" value="NEW1_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF14">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24984">
        <property type="entry name" value="HEAT_EF3_GNC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24988">
        <property type="entry name" value="NEW1_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="531"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="773"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1084"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="78"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1122"/>
            <end position="1139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1158" mass="128430" checksum="418C54466AE721CF" modified="2017-01-18" version="1">MPPKFNPYSAGSFVPGSGADPQQQYGGNQYSNDRSRGGSSYQGFQRKPMGYDQYIQQQSAGLNRAPRQNFQQQGQYDSTPSSISSTPLNSNLNSPSQSTTSLTSLNQTLDKLEISPITNNLQALKEAKTISDVKTEINSIVDSINENGISSLEEWKIEEIISKLIKPKNPALVREGGFVMISSIVRKFSGQTPKEAYFLTFLSIALDGLADKENNVKRASQAAIDSIYGSYPIEALASIVLVEILKYLKSSAKWQSKVGALKLVDKIVEDSPGDLIELKFKEAVPVLTDVSVDFKPELAKVGLKTMSTFVKVLDNLDLQPRYDMIISTLADPNKVPDCIKSLSSVTFVAEVTEPALSLLVPILVRSLNLSSSSQEQLRQTVIVVENLTRLVNNKFEIESFIPQLMPGVQKVVDTAALPEVRELAEKALKVLKDDSIDDGKFSGRLPHEEGKNYLSADLVNNDETVYEYLISILVADANVNDWNRLKEYLNLALPQYIEDESEISKIAEETVTKLRALFTPDAVASNEDDGIEIVNSDFSLAYGGRMLLNKTNLRLLKGHRYGLCGRNGAGKSTLMRAIANGQLEGFPDKSELRTCFVEHKLQGSEADMDLVSFIGSDPELAQVSKEEIAKALEEVGFDQERREQHVGSLSGGWKMKLELARAMLMKADVLLLDEPTNHLDVGNVKWLQDYLVSHDEITSLIVSHDSGFLDTVCTDIIHYENKKLAYYKGNLSEFVKVKPEGKSYYTLTDSNVKMAFPPPGILTGVKSNTRSVARMSNVTFAYPGAAKPSLSNVSCSLSLSSRVAILGPNGAGKSTLIKLLTAELIPNEGKVEKHPNLRIGYIAQHALQHVEMHKEKTANQYLQWRYRFGDDREVLLKESRKISEEEKEQMAKEIDVGDGRGPRQLEAIVGRQKLKKSFQYEVKWKFWLPKYNSWVSKELLIENGFDKLVQKFDDHEASREGLGYRQLIPSVIRKHFEDVGLDGDIADHTPMGSLSGGQLVKVVIAGAMWNNPHLLVLDEPTNYLDRDSLGGLAVAIRDWSGGVVMISHNNEFVGALCPEQWIVENGTMVRKGFAAVDGTSFEDGGSGAVSKDGSPAPSVPQTSNDDDSPANIKVRTRKKKMTRNEKKLREERRRLRHIEWLSSPKGTPKPVDTDDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P001</accession>
    <name>A0A1E3P001_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_69022</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037801.1">
        <property type="nucleotide sequence ID" value="XM_019184153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P001"/>
    <dbReference type="STRING" id="683960.A0A1E3P001"/>
    <dbReference type="GeneID" id="30201399"/>
    <dbReference type="OrthoDB" id="3980478at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF50">
        <property type="entry name" value="HEXOSE TRANSPORTER HXT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="68"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="322" mass="35654" checksum="F72FC4975B9E46C4" modified="2017-01-18" version="1">MTKPTGFKESSIPIPPPLTMELQDNRPPLYSLESDMDLINANNQPPISPTLLQVDLKLIWSFRKVIFLSLLISFSGFLFGYDTGVIGGIINMKSFLHRLGHLHYNESTDTMEYYLESYKTGLLVSSYHIGCITGGFTIARLADTLGRKLPLLISMMVYIIGIIIQITSQLSGKWYQFLIGRATSGLCIGSVAVLAPMFISETAPTVIRGSCTTLYQLSICIAILTGSIVVYACKEMYNNQAEWIIPLSIGIGAALAVCLGIISTPESARYLISIGEYEKARISLKKVEDPDIEGTLQILLEKLSIEHNASKIPFWEIFKKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P053</accession>
    <name>A0A1E3P053_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_79167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037827.1">
        <property type="nucleotide sequence ID" value="XM_019184846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P053"/>
    <dbReference type="STRING" id="683960.A0A1E3P053"/>
    <dbReference type="GeneID" id="30202092"/>
    <dbReference type="OrthoDB" id="284473at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="496" mass="56958" checksum="BABBB81E2E111116" modified="2017-01-18" version="1">MSQNLTEYLSRRANGNGNVKSEQKTQVKEQEGNDDNGSSVVLEKNNGNKNGWESLEVLTKNFHDTKTLPPPLSPTLPNVYGGDELKLEIPKMLSPTLPDIFNEKKSNGSSIKQPVPQRPSGKIRIEDDEDEDSASSEEINEIKVEEKIPNPPVKRKSFEKIEHFGVHSYKLTETTNSKSLLLTLHIPSYYKNKRQKVSKPIGLGIKRVPEKSSSVVTEKSNNDHQQKVKKASIFKTSSKDPSPPTSASHKDSTKDSKLDHTSLMAKNEEYLLIAKEKKHRGDEQRTKNLKFSLACFLDSIIIYLVGFNYEDQSRRLLKKLYNDKTWLSLISLIDHALKLSTDNGIVDVAGLCLQIKAVVYEHVCKILDEFIQLNNVKRRKAKTDADILKIDDNLVNHFKNFSRYKELSKRSFIQSERYLSVFKIQRKYPSVFEFFENQPERNRLIKDVDFFLDPTRDSIQLPINTGVSLKEICAYSLKVIKQWCQDEQVKYNDWTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0I5</accession>
    <name>A0A1E3P0I5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_34659</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037624.1">
        <property type="nucleotide sequence ID" value="XM_019182214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0I5"/>
    <dbReference type="STRING" id="683960.A0A1E3P0I5"/>
    <dbReference type="GeneID" id="30199460"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03048">
        <property type="entry name" value="GST_N_Ure2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF8">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE GSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01151">
        <property type="entry name" value="Main.2:_Nu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="19"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="112"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="26724" checksum="131DB404168DF6CC" modified="2017-01-18" version="1">MSSTNLDLADPEHPKDLKAPYLKLYSLETPNGQKATLYLKLLGIDYTLRRLDIRTNIQKEPWFIAINPNGRIPTLSDVDAQGKQTILFETGAILLYLGEKYDKERKYYYSLNDEHYWDQIKWLTFQIASHAPYQGQANHFQHYAPEKIEYGIKRYKAETERVFGVYELRLKENNGWLVGDHLNIADIAAFPWVKIYAYNDIDIDQFPHLKAWIEKIKNIDGVQAGLDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0K7</accession>
    <name>A0A1E3P0K7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glycosyltransferase family 15 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_64599</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037669.1">
        <property type="nucleotide sequence ID" value="XM_019183768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0K7"/>
    <dbReference type="STRING" id="683960.A0A1E3P0K7"/>
    <dbReference type="GeneID" id="30201014"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF6">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="402" mass="47521" checksum="F34D80D4631CBD1A" modified="2017-01-18" version="1">MLKIQSTSSNLFRLKLNERIIRIAVFSAGIIGLIFLLSNTTSGPTYVRSVKKPTTHPASNAQSGTGDHLINIPLAETTYVRENATFVSLARNEDLWELVHAIRSVEDRFNRQYKYDWVFLNNEEFNHEFKTVMTTLVSGEAKFGLIPKDHWSYPSWISTEKAAKTREDMKNIIYGSSEPYRHMCRYESGFFYRHPLMLDYKYYWRVEPSTKLHCDVKYDVFKFMKDNNKDYGFTITIHEFEETIKTLWKTTQDFIKEFPQYIAKDNLEKFVSNDGGKTYNLCHFWTNFEVGNMDFWRGEAYSKYFEYLDKNGGFFYERWGDAPVHSIAASLFLPQDRIHFFKEIGYFHGPYHNCPIEDDVRDENKCMCNPNDDFTWKDYSCTRLYYDVKGLQKPKGWDKHTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0R3</accession>
    <name>A0A1E3P0R3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Maintenance of telomere capping protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_63531</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in telomere capping.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the MTC6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038238.1">
        <property type="nucleotide sequence ID" value="XM_019183566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0R3"/>
    <dbReference type="STRING" id="683960.A0A1E3P0R3"/>
    <dbReference type="GeneID" id="30200812"/>
    <dbReference type="OrthoDB" id="5573651at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051008">
        <property type="entry name" value="Telomere_Capping_Maintenance"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057530">
        <property type="entry name" value="TIM-barrel_MTC6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35518:SF2">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35518">
        <property type="entry name" value="MAINTENANCE OF TELOMOERE CAPPING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25506">
        <property type="entry name" value="TIM-barrel_MTC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133632" description="Maintenance of telomere capping protein 6" evidence="11">
        <location>
            <begin position="18"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="485"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="MTC6 partial TIM-barrel" evidence="12">
        <location>
            <begin position="22"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="534" mass="60022" checksum="20BA081E5755AEB1" modified="2017-01-18" version="1">MLLLPFILLLLLRSVFAQSNWPGLSSDKVIAIRSQRDVAANITIDQLSLTGVRLSKVLFDREGYVNDTLIDVRNLLNVGVQTLVIDAYWNENLATFQLCPVDMSSNSTTSSYGNSVMYYEDTQLSYKCDSILTLKGLIDIVDNFASSTDTNLSANLIVLIFNIHQLKTRNSHISNDPISTNDTLYSTLTRSLGNKLYTPLNLDSDRENNRTLLSNEGPGKGFPSSQYFLFNNERRVISTIWQNNLPSNSTYNTTADFNVIFTPKSLNSSFTSLTNTTIPLTTTQYIARSNESWRFAYDDQQQPFTNTSIKRLISEGYSPILNSPISSNSEISNIFSMSLWSWNTSEPLQPEQARQISADGDNTRNTQSAYKCAALTQDGWVVSNCYQDKYVVCRKNSRAYEWEISDSKGVYFHAEDACPGDTVFSVPRTALQQTSLINYLGRLGSNYSVWIDMNSIAISDCWVTGGPYASCPYQKVSSSRNFVQMLAPAAAFCGFILFGILLLRLKRVPVQDNRKHWKKLLANYTENEYEGVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0T3</accession>
    <name>A0A1E3P0T3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MOSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_63506</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038212.1">
        <property type="nucleotide sequence ID" value="XM_019183562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0T3"/>
    <dbReference type="STRING" id="683960.A0A1E3P0T3"/>
    <dbReference type="GeneID" id="30200808"/>
    <dbReference type="OrthoDB" id="17255at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005303">
        <property type="entry name" value="MOCOS_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03476">
        <property type="entry name" value="MOSC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133619" description="MOSC domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="MOSC" evidence="2">
        <location>
            <begin position="210"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="379" mass="43864" checksum="D3EF07E99F4CC7B2" modified="2017-01-18" version="1">MILVALLFFSALVSFICLPQLIKALNLSTTAPNDQLSNLLRRLLNLRFYFIAKILFFKAPTRAGKIDKIYVYPLKSVSYISPTKWEIDEHGLKHDRQYMIGFWDSKANCYQAYTLRNAPRLSLVSIDYDLEENWFKFTYPKLDGSKSFFKLPCNVTDEFLAKHIVNIDESDQPSRKITDLWGIKFDSINLGSNLIPQDFYDSMGLNRDGTTLLYSSKDRTVKTAHPKDLKQMRKVLFQDYFPIHIISQSSIDELNQRMKKSGVKDRIVEPLQFRPNVVIDGNAIELDYWYKVFINGHLWSIVQKTPRCSIGNVCLDKGEFDKSNSVTRTLRSYRRIDPGDKNGFFLGDDAIHHDSGYFINVGDEFYLKQQKISTSLPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0V5</accession>
    <name>A0A1E3P0V5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Hyaluronan/mRNA-binding protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_32373</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038316.1">
        <property type="nucleotide sequence ID" value="XM_019181948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0V5"/>
    <dbReference type="STRING" id="683960.A0A1E3P0V5"/>
    <dbReference type="GeneID" id="30199194"/>
    <dbReference type="OrthoDB" id="2122308at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006861">
        <property type="entry name" value="HABP4_PAIRBP1-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04774">
        <property type="entry name" value="HABP4_PAI-RBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hyaluronan/mRNA-binding protein" evidence="2">
        <location>
            <begin position="30"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="110" mass="12698" checksum="7364E5A275C9E822" modified="2017-01-18" version="1">MTRTQKWTYHEKRAEPHMFTHNGHPDQNPQHTKKNGAGRGNWGSPGDEINDLIDDGEIPPVFNKRRGSNASAHEVKFDAIQHSHDHIDEDDEDDYYDEEEQSTNESDESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P127</accession>
    <name>A0A1E3P127_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_32035</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038358.1">
        <property type="nucleotide sequence ID" value="XM_019181912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P127"/>
    <dbReference type="STRING" id="683960.A0A1E3P127"/>
    <dbReference type="GeneID" id="30199158"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14127">
        <property type="entry name" value="STKc_CK1_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000160">
        <property type="entry name" value="Casein kinase I 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="16"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="311"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="334"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="421" mass="47788" checksum="43ABB798AD56A71C" modified="2017-01-18" version="1">MSSSQAQSANVIGIHYRVGKKIGEGSFGIIFEGVNLLNNQQVAIKFEPRRTEAPQLRDEYRAYRILAGSQGIPRAYYFGQEGLHNILVIDLLGPSLEDLFDWCGRKLSPKTVAQVAKQMITRVQTIHDKDLIFRDIKPDNFLIGKAGSTNANTIHIVDFGMAKYYRDPKTKQHIPYRERKSLSGTARYMSINTHLGREQSRRDDLESLGHVFLYFLRGSLPWQGLKAPTNKQKYEKIGEKKRNTPVSELVQGLPSQFGDYLTYVRNLTFEQNPDYDYLRALMDAAIESVGETADGYYDWMSLNGGKGWDAQINKKPNLHGYGNPNPRANQTNPLQGQQQAQQAQQQQQVQQAHLKQKVQSLNNSRSRTPKVNQTNYLLNSSQYAINSNKASDQQQNLINDQTDIRSTTSEKGFWSKICCCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P147</accession>
    <name>A0A1E3P147_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Glycosyltransferase family 71 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_13272</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038332.1">
        <property type="nucleotide sequence ID" value="XM_019180566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P147"/>
    <dbReference type="STRING" id="683960.A0A1E3P147"/>
    <dbReference type="GeneID" id="30197812"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF1">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="451" mass="51771" checksum="D3D5ADB23DF908DE" modified="2017-01-18" version="1" fragment="single">QLFSKLFQIINEADPKIPRLDTYKTDQRVKNIRFDEETDLVFTKEYLQGFLDVKPEEVEALQKSHSQVVANFPKHANQLYKGEGIVYVGGGKFNWLSLMSIRTLRNIGSTLPVEVLIPTNEDYEQELCEIVLPSMNAKCLLLPDVLGEETMNKFEIGGFQYKALAILVSSFETVLLLDADNIPAYIPDHIFHQDPLKSKGLVIWPDFWRRATSLDYYKIANITVDENTRVMNGVTEKEKLQTDIPFHHFKGAIPDPTSESGQVLINKTMHLDTMFLSLYYNIYGPLYYYPLLSQGGLGQGDKETFLSAAVASNKPFYQMHENLVALGRHDDEGNFHGVGMGQFDPVQDFNKHETGQDDGTDPRILFIHANFPKLDPVKLENEKTMFDNDKRVRMYGPNMASRIGYDFEMFQWNNMHYLLCESHLNLKLFEKENITQVCANIQVHQDFLKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P196</accession>
    <name>A0A1E3P196_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">mRNA m(6)A methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.348</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_33207</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an adenosine in mRNA + S-adenosyl-L-methionine = an N(6)-methyladenosine in mRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55584"/>
            <dbReference type="Rhea" id="RHEA-COMP:12414"/>
            <dbReference type="Rhea" id="RHEA-COMP:12417"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74449"/>
            <dbReference type="EC" id="2.1.1.348"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MT-A70-like family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.348" evidence="1"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038305.1">
        <property type="nucleotide sequence ID" value="XM_019182043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P196"/>
    <dbReference type="STRING" id="683960.A0A1E3P196"/>
    <dbReference type="GeneID" id="30199289"/>
    <dbReference type="OrthoDB" id="10262526at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001734">
        <property type="term" value="F:mRNA m(6)A methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902974">
        <property type="term" value="P:meiotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000221">
        <property type="term" value="P:negative regulation of pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829:SF7">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="343" mass="39449" checksum="9173BE93C2BEDC13" modified="2017-01-18" version="1">MENPNLTKLSSLLNYESSTVQLSREKLRFTTPKSPFIETCNNPQHLQYHKSFDHCFQTKIHFIPIITNQTDPSLGDCSYLDTCHKMDSCRYVHYTKHIPKNFAQNEEQICNSFNNQLLNNEKISYWNINGSISTISRCQLSPQWINCDVRKFDFTILGQFAAVIADPAWNIHMNLPYGTCNDTELNSLPLSSIQEEGIICLWVTGRTIEIGKKALSNWGYKVQNEIIWIKTNQLGRTICTGRTGHWLNHSKEHLIVGVKGTPKWLNKNIEGDVLVSGTRETSRKPDELYGMVERLVGPHARKLEIFGRDHNTRPGWFTIGNQLKGNQIVEYDVLERYNASMNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1G2</accession>
    <name>A0A1E3P1G2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">cAMP-dependent protein kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_79764</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Tetramer, composed of 2 regulatory (R) and 2 catalytic (C) subunits. In the presence of cAMP it dissociates into 2 active monomeric C subunits and an R dimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038452.1">
        <property type="nucleotide sequence ID" value="XM_019184945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1G2"/>
    <dbReference type="STRING" id="683960.A0A1E3P1G2"/>
    <dbReference type="GeneID" id="30202191"/>
    <dbReference type="OrthoDB" id="417078at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046580">
        <property type="term" value="P:negative regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046827">
        <property type="term" value="P:positive regulation of protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097271">
        <property type="term" value="P:protein localization to bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010603">
        <property type="term" value="P:regulation of cytoplasmic mRNA processing body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd12098">
        <property type="entry name" value="DD_R_ScPKA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000039">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000118">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012198">
        <property type="entry name" value="cAMP_dep_PK_reg_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003117">
        <property type="entry name" value="cAMP_dep_PK_reg_su_I/II_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02197">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000548">
        <property type="entry name" value="PK_regulatory"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00394">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47391">
        <property type="entry name" value="Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0114">cAMP</keyword>
    <keyword evidence="4 8 9" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="11">
        <location>
            <begin position="224"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="11">
        <location>
            <begin position="342"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="69"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="133"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000548-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ59245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="456" mass="50411" checksum="FE58AE378F74F23F" modified="2017-01-18" version="1">MSLPKEYINELNELNKEIQRKNPQDILQFCFNYFQSRLEEQRKQLWSQEAKARAAGISLFPSRKLNSIQDEDAKGSSPLFKNSFVNDDPHSKDLVGHDHDASTSSSGDNNIFKSGFHVGEENKSNIANTVDPIAAPAQGRRTSHVVGHSPSSNTSTPPPSASSNHHHSQQLHNIPSTFNANRRTSVSAETLNPNRLKDDWKPPIIAKTPDQLSRLSNSVVKNFLFSSLDEDSLKTVIGALEEIKKPAGSEVIKQGDEGDFFYVVESGIVNFYVNGDKVNSSGPGSSFGELALMYNSPRAATATAETDLVLWALDRLTFRRILLAKTSKKRQLYESFLKEVPVLSRLSLFERSKLADALETESYKAGDVIIKEGEVGENFYLIETGEANIVKNEGGLIGSVKRGDYFGEVALLNDLPRQASVIAKTDVNVATLDKRGFQRLLGPAVEVLKRQDPTKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1J3</accession>
    <name>A0A1E3P1J3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_63290</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037989.1">
        <property type="nucleotide sequence ID" value="XM_019183522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1J3"/>
    <dbReference type="STRING" id="683960.A0A1E3P1J3"/>
    <dbReference type="GeneID" id="30200768"/>
    <dbReference type="OrthoDB" id="759142at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133614" description="GOLD domain-containing protein" evidence="9">
        <location>
            <begin position="20"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="10">
        <location>
            <begin position="33"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="211" mass="23826" checksum="012861F3ACEDB9F4" modified="2017-01-18" version="1">MRSTGYLLFLLSIIASAVALHFEIDAVEETRPPMCIRDFVSEGQLVVVNIHSSGSVGDGQVLNFNIRDSVGNEYRKKKDFAGNVRVAFTAHSSAAFDVCFQNVLQQRGRKLSRELEVDIEAGSAARDWNAIQAAEKLKPVEVELRKVEELTDEIVGELEYLKAREERLRDTNESTNRRVRNFSIVIIIALISLGGWQIRYLGNYFKAKHII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1M0</accession>
    <name>A0A1E3P1M0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8032 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_63315</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038014.1">
        <property type="nucleotide sequence ID" value="XM_019183525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1M0"/>
    <dbReference type="STRING" id="683960.A0A1E3P1M0"/>
    <dbReference type="GeneID" id="30200771"/>
    <dbReference type="OrthoDB" id="5599902at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058345">
        <property type="entry name" value="DUF8032"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22949:SF0">
        <property type="entry name" value="RE27538P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22949">
        <property type="entry name" value="WHITE COLLAR 2 PROTEIN WC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26087">
        <property type="entry name" value="DUF8032"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8032" evidence="2">
        <location>
            <begin position="286"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="214"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="397" mass="44552" checksum="85A4FF9E25598FDA" modified="2017-01-18" version="1">MNYDQLNLDSSVGGSGGFNQLQTYQTYSNNSGAGQVDYNNHETQYQGYFVETFNSQMNYSNLHPSTFQSSAIYENLTSATTTPLTEFPIDTSAFDKYEEEFVPVSNTQNDDNGFASFQLNKKVNDFDQFPTISTEYSSESCSISKTASSSSSCQPLLSSCTQQNDINSYFITSAPTTSFNSPLSTPLASPTSTQFSRLNHPLKSPTSPRKSRPATTATSSISSRRSSITSNKVCKTYREKSASLSSPSSHSHTSHHFHHSLQHTTQPGPIPASKPQLTTDLTTGEELLTFSYSKQKIIRNFTIKCPPPNPSEALSELSQQFLLENCVYPRAMCSFEDYKGNRYQYEKECNEIGWSLASLNPEIRDHRGLIQRAVDSWRNTRKDKKLRSRRVRKSELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1S3</accession>
    <name>A0A1E3P1S3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA repair metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_79672</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038351.1">
        <property type="nucleotide sequence ID" value="XM_019184930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1S3"/>
    <dbReference type="STRING" id="683960.A0A1E3P1S3"/>
    <dbReference type="GeneID" id="30202176"/>
    <dbReference type="OrthoDB" id="262529at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16273">
        <property type="entry name" value="SNM1A-1C-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011084">
        <property type="entry name" value="DRMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF6">
        <property type="entry name" value="DNA CROSS-LINK REPAIR 1A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07522">
        <property type="entry name" value="DRMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA repair metallo-beta-lactamase" evidence="7">
        <location>
            <begin position="403"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="21"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="130"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="544" mass="63149" checksum="63681ADC0E8E756A" modified="2017-01-18" version="1">MGQSNLLDLLLLSKRNQRSNKRNYDGEMIDLTSDGEDECSMANGDEEVIQDEETEKGEKLFVKEEEDGTKENSVGLDQVTPEVQESDPTPDLNETIPCPVCQLNISKLTLSDRESHVELCLLQPTTHKSIVAPSPKKSKPKQESSKKPRQPKPRTKPIPDFKILTFKNNHKINVDGFQFMDHPEVDQYFLSHYHSDHYIGLGKNWNQGKIYTSKTTSNLLQTVMKIPQDKIVALPFNEPFKIHENIEVILYDANHCPGGAIFLFQEFDPEKKIIKRILHTGDFRVSLNHINLFKSIFIDEIYLDTTYLNPVYTFMPQRKVVSRSSNFIANIINQTIKKNSILNYFKESNDYLIIIGAYSIGKEKLYINLAKTIKSKILITKDRYKMLSCIDGFTDLDIFQFEESKDCKIQIVSINKLTDLKFLSKFQNKKIIKIKPTGWSFNTFKKDTNIKDLSFDEILQKTFINNEFKDFENQLSNQFQKSSSLSIPYSEHSSFKELSIFASFLKWGEIIPTVNIGNEYFPKWFEIWKNYELDESVVNEYFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P215</accession>
    <name>A0A1E3P215_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_23253</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038733.1">
        <property type="nucleotide sequence ID" value="XM_019180982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P215"/>
    <dbReference type="STRING" id="683960.A0A1E3P215"/>
    <dbReference type="GeneID" id="30198228"/>
    <dbReference type="OrthoDB" id="3981033at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071248">
        <property type="term" value="P:cellular response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071467">
        <property type="term" value="P:cellular response to pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000181">
        <property type="entry name" value="C2H2 type zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF44">
        <property type="entry name" value="ZINC FINGER PROTEIN 271-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="82" mass="9580" checksum="D51C74E82BE19147" modified="2017-01-18" version="1" fragment="single">PHCLKIFSRLYNLKSHLKTHSDEKPFNCSYCDRKFARNHDRKRHELLHQGEKKFQCGGILGDKVTKWGCGKKFARADGLGRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P240</accession>
    <name>A0A1E3P240_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">TrmE-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_63908</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038620.1">
        <property type="nucleotide sequence ID" value="XM_019183634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P240"/>
    <dbReference type="STRING" id="683960.A0A1E3P240"/>
    <dbReference type="GeneID" id="30200880"/>
    <dbReference type="OrthoDB" id="188276at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070899">
        <property type="term" value="P:mitochondrial tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04164">
        <property type="entry name" value="trmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14858">
        <property type="entry name" value="TrmE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.120:FF:000007">
        <property type="entry name" value="tRNA modification GTPase GTPBP3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.430">
        <property type="entry name" value="tRNA modification GTPase MnmE domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00379">
        <property type="entry name" value="GTPase_MnmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031168">
        <property type="entry name" value="G_TrmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018948">
        <property type="entry name" value="GTP-bd_TrmE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004520">
        <property type="entry name" value="GTPase_MnmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027368">
        <property type="entry name" value="MnmE_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025867">
        <property type="entry name" value="MnmE_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00450">
        <property type="entry name" value="mnmE_trmE_thdF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003661">
        <property type="entry name" value="PRK05291.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42714">
        <property type="entry name" value="TRNA MODIFICATION GTPASE GTPBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42714:SF2">
        <property type="entry name" value="TRNA MODIFICATION GTPASE GTPBP3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12631">
        <property type="entry name" value="MnmE_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10396">
        <property type="entry name" value="TrmE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="GTP-binding protein TrmE N-terminal" evidence="9">
        <location>
            <begin position="26"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="MnmE helical" evidence="10">
        <location>
            <begin position="149"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="G" evidence="8">
        <location>
            <begin position="249"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="54742" checksum="CD028AC1D6581BC5" modified="2017-01-18" version="1">MFTIKRLIQKGKPSLVRYSSTSHLPTVYALSTHFARSAIGVVRISGTHSQFVLEQLTKQALPKPRIASVRKLYQPNTSNLLDESLAIFFKSPKSYTGEDLVELHLHGGTAVIQAVLKSIESLHTVERPIRYAEAGDFSKRAFQNGRFDLTEIEGIRELIDAETESQRRGVMSSVTGGNKDKFLQWREKMVDSIGLLTAIIDFAEDAEIEDIEKIYNNVDSKIKELDSDIKEFLERTQRSEVLLKGIKLTLLGPPNAGKSSILNKLANEEAAIVSDIAGTTRDIINVPLNIQGYKVVVGDTAGIRDLAKADRIEVEGIKRAKMQAQIGDLVVVVLGTDNMKIEDELADMVKLLNSEKKVLVVLNKCDLVKDDEVSTLIKNISSRLNVPMENFLPVSCIDSKGLEQLSSQLVSEFKRISFTETTEPLIITQRVKDILINDVLMGFEEFQRYKDLDDVVIASESLVQSVEGIGKITGETVGVEEVLGVVFSSFCVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P267</accession>
    <name>A0A1E3P267_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_62177</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">G proteins are composed of 3 units, alpha, beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G protein gamma family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038796.1">
        <property type="nucleotide sequence ID" value="XM_019183321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P267"/>
    <dbReference type="STRING" id="683960.A0A1E3P267"/>
    <dbReference type="GeneID" id="30200567"/>
    <dbReference type="OrthoDB" id="3980512at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120171">
        <property type="term" value="C:Cdc24p-Far1p-Gbetagamma complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031680">
        <property type="term" value="C:G-protein beta/gamma-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000003">
        <property type="entry name" value="G-protein complex gamma subunit Ste18/GpgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041848">
        <property type="entry name" value="Ste18_fungal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28189">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28189:SF1">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="12">
        <location>
            <begin position="31"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="112" mass="12518" checksum="2EC420C6BC3CC1CF" modified="2017-01-18" version="1">MSEPSTPLTNSGSFSQHSSPNKKSAIQQKLLILKLKRINELNNRLKDTLSKDRIYASNASYSIIDYTQSHKDYAIPEIWGNLQPGENPFRNNRFQQKTGGGGQDQGGCCTIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2D6</accession>
    <name>A0A1E3P2D6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_69830</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038695.1">
        <property type="nucleotide sequence ID" value="XM_019184184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2D6"/>
    <dbReference type="STRING" id="683960.A0A1E3P2D6"/>
    <dbReference type="GeneID" id="30201430"/>
    <dbReference type="OrthoDB" id="2095648at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052301">
        <property type="entry name" value="SCF_F-box/WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14381">
        <property type="entry name" value="DACTYLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14381:SF1">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="9"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="572"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="583"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="629" mass="71302" checksum="587A4D630887D05A" modified="2017-01-18" version="1">MDQVTKPEMTSFLKLPAEIVIKILSYLDVSDLYNIAETCQSLNHIINDEELWKNLFMKRFHTNHFSSSSNSFRYSVELFERNEIIHRWKKSTGMHRIFTINSTAIEKVVLNYPKLLSFSDQGDINISSLERAKTETTIPMTTPNGCTSYSFNTSASVFGRIDGKIFGKLLATRSFLSSMSEFNRAHEGMVTTIHNDDSMCYSGDEKGKLFTWDLKNGEFIQEFDVSNEAIISVKGFNNLIVALDSQNVYIIESDTVRSIPHSSGSDFFEVDFSGGLVIVGNHHELSVYSYHRSSFGRQTKLSVGEQDEIWKVALETKTISSRDLKIAGYDGCNFGVVTKLGRVMTFNIRDLRLKASTGLLQPQCEIVPIFDTLRIPDGIPPISSISINSTVVLLGSYNGFAAVYEVLTGEFIKLVSNRIPKRHLPLTQPPYLIPVKFVELSPKNQTNGVLIVNNVVQYFQFGKALHDVQNSQKKKKLLTGVLSDRKVKLKKKIKHEVDELDYEAHEQYKKDQLLDKYNGVDLTEWEEMELAMVLNHSLNDQSHNTGKELEDEDSDEVDAELSRALELSKLEHKNDNWDDGGASSDNYTGAIEQSSVTKREPIESNATRVIEDDDFEKQIQEALRRSLYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2F2</accession>
    <name>A0A1E3P2F2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">beta-glucosidase</fullName>
            <ecNumber evidence="4 11">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_29918</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Glycan metabolism; cellulose degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="4 11"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038730.1">
        <property type="nucleotide sequence ID" value="XM_019181666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2F2"/>
    <dbReference type="STRING" id="683960.A0A1E3P2F2"/>
    <dbReference type="GeneID" id="30198912"/>
    <dbReference type="OrthoDB" id="416222at2759"/>
    <dbReference type="UniPathway" id="UPA00696"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.300:FF:000002">
        <property type="entry name" value="Probable beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050288">
        <property type="entry name" value="Cellulose_deg_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019800">
        <property type="entry name" value="Glyco_hydro_3_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715:SF2">
        <property type="entry name" value="BETA-GLUCOSIDASE F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00775">
        <property type="entry name" value="GLYCOSYL_HYDROL_F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 11 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 11" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type III-like" evidence="12">
        <location>
            <begin position="728"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="811" mass="90764" checksum="15A966F8BDC60DB0" modified="2017-01-18" version="1">MTVLEKVNLTTGIGWGSGPCIGNTGGVPRLDIPPLCLQDGPNGVRFTDFVTSFPSGLATGATFNKDLMHRRGKAIGREAKAKGVHILLAPTIGPIGYKAQGGRNWESFGSDPYLQGIAGALTVQGIQEEGVVAVARHFIGNEQEHFRQENEWTGGDWDRLTDSIDSQIKDRVMHEIYLWPWADVVHAGVGGVMCSYNRVNETYACENSYLLNYLLKEELGFQGFVVSDWGAQHSGVESALAGLDMTMPGEIFGDWCSGKSYWGPLLTRSIYNDTIPQSRLNDMVTRILAAFYSIDIKLPSEQREEDLPNFSSWSFHTYDQEFPFQSYGPIKQTNYHIDARDDFTEKTALQVAREGIVLLKNDAHHLPICTNDGVRRLLVAGKGAGPDTKGFNSKDQQSADGALFSGWGSASVNAPYGITPWEAIAKKAREREMVIDYTSDEYEFDHVDEIAEYADMCIVFGFADSGEGYIEVDGNFGDRKNLTLWQNTEELIQHIANRCHKTVVVVTATGPVNLESFVDHENVVAVLYTPPLGQFFGAAIADVLFGDDNPSGRLPFTIARKDDQYVDIIDKIPLKGKPQDDLNRLLDYRYFDENGIRPRYEFGFGLSYTKFSLSELSIKEILPPTTDLPCPAPYLDEYVYAEEKLKDVDDALFPFRDFTPVPGYIYPYLFNEKIYSVDEYEYPEGYTEEDYYEEPLAGGSLGGNEALWNVLYSVKVEVTNIGDSEGAYVAQLYIDYPNGYGDIKTPRQLRGFDKVYVKPGETAKIHFDILRRDISVWDTEDQSWVINPGTYKLYVGSSSRRLELAGEVEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2I2</accession>
    <name>A0A1E3P2I2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">COX assembly mitochondrial protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_17719</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for mitochondrial cytochrome c oxidase (COX) assembly and respiration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the CMC family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038906.1">
        <property type="nucleotide sequence ID" value="XM_019180758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2I2"/>
    <dbReference type="STRING" id="683960.A0A1E3P2I2"/>
    <dbReference type="GeneID" id="30198004"/>
    <dbReference type="OrthoDB" id="6224010at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013892">
        <property type="entry name" value="Cyt_c_biogenesis_Cmc1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08583">
        <property type="entry name" value="Cmc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="90" mass="10768" checksum="663365F82E4570A6" modified="2017-01-18" version="1" fragment="single">IMNSHNAKLPVWMLTPMEEKEARKRWKEYAYKQCDEVVQKFAKCSKENGVGVLWNCRSARDDMNKCILRLQKPEELDRQRELLIAEKIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2J1</accession>
    <name>A0A1E3P2J1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_84758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019185774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2J1"/>
    <dbReference type="GeneID" id="30203020"/>
    <dbReference type="OrthoDB" id="4074030at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="61"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="85"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="177" mass="20642" checksum="0A8F7C5EC5B398B5" modified="2017-01-18" version="1">MPRLRKSIQKINQLDSDFPIDEDEQEELLELLKTDNIQTNKLFINIFTIIYLIPIPLFIHLKYFRTSSTISFLSIVSLVLSLIKIRYLHVINFANFESSIVQWLSILYKPLVFNVLNLIISSIVLMVRYEQGVGDLNLLYFIPFLASVSSILLMYWILELDNGITSLSKLKYKYKSA</sequence>
</entry>
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        <name evidence="9" type="ORF">WICANDRAFT_63756</name>
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        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="GeneID" id="30200850"/>
    <dbReference type="OrthoDB" id="2802411at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659:SF57">
        <property type="entry name" value="PLASMA MEMBRANE PROTEOLIPID 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
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        <location>
            <begin position="21"/>
            <end position="137"/>
        </location>
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        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
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        <location>
            <begin position="96"/>
            <end position="137"/>
        </location>
    </feature>
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        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="123"/>
            <end position="137"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="15502" checksum="2C171EF2D944A656" modified="2017-01-18" version="1">MCDCILVLLALVFPPFPVWVKCGLCSAESLISIALSILGYIPGVIYALYVIAKYPIRYVVIDEEAQVYRCHPSHHRVQQGPPRVIIRERVGTPSSEQIISNSNNQFYGSTSNDNPPDYEEVLQQQQHQQQQHPPRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P365</accession>
    <name>A0A1E3P365_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peptide transporter PTR2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_78488</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
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    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ59855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_019184733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P365"/>
    <dbReference type="GeneID" id="30201979"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071916">
        <property type="term" value="F:dipeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="MFS peptide transporter Ptr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01023">
        <property type="entry name" value="PTR2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="382"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="420"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="503"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="534"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="563"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="651" mass="73093" checksum="6941598699009051" modified="2017-01-18" version="1">MEITNIKEKGFNLKEKISERLRPTDVEVSSNNTEENNADRYDELVEYNEKYPEPTAEDYTTLPKVLGHAPYATYLVCLVEFAERASYYGVKDRLNNFIQLKLPKGGNGAGAPPKGTQQNAGALGLGLQTASAITLLLTFLAYLTPLYGGYISDKKLGRMKTIWYGVWIGAISHVILIIAAIPSVIKGGNALAPTIISILTLALGTGFIKPNLLPLLFDQYPHHRDIIETKNDGKKVIVAREATLERMTLFFYWAVNAGAFLSLGTSYCAKRVGFWLAYLIPGIVYIIMIPTLMLLSPRLKKETPNGISILEEAFKVIRYTLKGGWIKRIKENQYWEYAKPSNIVARGEVESTEAVNKKGKKRISWTDQFVQDVKVTIDACKIFLFFIIYNINDVGIGGIQNSQANTMTTNGVPNDLINNFNPLTIIVFIPFLDYILYPLLRKLKIEFRPVHRIFTGFIIASFSSVAGAIIQWKVYETSPCGYYATNCEIGTGVSPISVWVETVLYVLAASSECFANTAAYEIAYTRAPDSMKGLVMALFLFMQSISAAISEACTSALIDPHLIWPFVATAIAGFISAFIFLWLYRDLHKVMHAERLEKEDKMKQEFLQYHDMKFNNENELEAVVSQKLSMEEERKQEQSVEGTVFVNSLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3H5</accession>
    <name>A0A1E3P3H5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_56178</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038659.1">
        <property type="nucleotide sequence ID" value="XM_019182772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3H5"/>
    <dbReference type="STRING" id="683960.A0A1E3P3H5"/>
    <dbReference type="GeneID" id="30200018"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06609">
        <property type="entry name" value="STKc_MST3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000499">
        <property type="entry name" value="Serine/threonine-protein kinase KIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046409">
        <property type="entry name" value="PDC10_dimerisation_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF10">
        <property type="entry name" value="FI20177P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="13"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="362"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="374"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="459" mass="52520" checksum="236198103E6DCF83" modified="2017-01-18" version="1">MGLEDTIEPTELYEIEECVGRGNFGDVYKAVDKKSSKVVAIKVVNLEETDDEIDVLVQEISFLSQLRSNYITNYYQTFVRDVCMWIVMEYCGGGSCADLLKCHKFLNEDVVCFIVRETLKGLEYLHSERKIHRDIKAANILLTSSGNVKLADFGVSGQITATHVRKDTFVGTPFWMAPEIITRKTGYNEKVDIWSLGITTIELVTGSPPYSDHEPMKILFQIPKNPAPLLTGSTYSDYLKEFVRFCLIKNPSHRPSASILLKTKFIRQCRRHINLVPLIQQKDKWMQTHRPNAKKPRYHIDDKLYTKSEASFKWNFTNTKRQKRASHTNSDLENIYKELMESSRPNSAGDSTVDEISPVTCVDSSETDLTSPEYNKEEENGQKDQADKLQQIDYLNEVVLHCFKRVHHRAKTSDTKDAVSNLGKQFVLAERTQPGLAEALSEEIWLRMCRLRGDSNSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3L1</accession>
    <name>A0A1E3P3L1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Meiotic sister chromatid recombination protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_31834</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038699.1">
        <property type="nucleotide sequence ID" value="XM_019181886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3L1"/>
    <dbReference type="STRING" id="683960.A0A1E3P3L1"/>
    <dbReference type="GeneID" id="30199132"/>
    <dbReference type="OrthoDB" id="2527403at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990166">
        <property type="term" value="P:protein localization to site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018803">
        <property type="entry name" value="Ish1/Msc1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10281">
        <property type="entry name" value="Ish1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133644" description="Meiotic sister chromatid recombination protein 1" evidence="1">
        <location>
            <begin position="19"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="498" mass="57413" checksum="840714D1BF11D3E8" modified="2017-01-18" version="1">MKVINLLSVLLLSTSSIALFSSTVLDSWDLNDLKEYLSDNSVPFDEKKATVEQIKDLSQKHWELQHSKSQSSWFDPNVVKQKVLNYYNGATDSLTSNPATSSSSVDYSNLKDWVFATWGIDQLKSTLKKSDIKFDPEAKRDDLVKLAKSNYNKIAKKYNASGNYPGDWLYSNWDKKSLKKWLDQYGIDYSSFRDSQKDLIRKVRQNSYQAAQVAEEERQGILESLDLSSKALFDKAGTVRDDIFNTWSSSQLYKWLKTHQVDVEESLKTNKEELALLAQKHKDNLKDDIEYWSGKASKSASPFLTKSSDKVDNVINDTFFVGVESWSRDRLKAFLAARGVSVPIFATKYELIQLVKDNKFKPIQNFNNDYFFQGWSKENIQKWVDEQNDAASKTSKASGYANQASNHFSTARDSFFDYWSDVELREYLSSFGIYDTKNFRHDQLLDLAKTNTRWFISGANYDTGSAIANAKLTGVNLWNKIVYYVKTWYNTIYYTFNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3L8</accession>
    <name>A0A1E3P3L8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_29884</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039288.1">
        <property type="nucleotide sequence ID" value="XM_019181663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3L8"/>
    <dbReference type="STRING" id="683960.A0A1E3P3L8"/>
    <dbReference type="GeneID" id="30198909"/>
    <dbReference type="OrthoDB" id="10258445at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090324">
        <property type="term" value="P:negative regulation of oxidative phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052000">
        <property type="entry name" value="ETFRF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21024:SF0">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN REGULATORY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21024">
        <property type="entry name" value="GROWTH HORMONE-INDUCIBLE SOLUBLE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="66" mass="7976" checksum="696F047ED36D9936" modified="2017-01-18" version="1">MDPRVRNLYKRLLWIGRDYPQGYDYFKGKLRKGFIKNRQLEGQEVEKALEKGEFIYKGESNCFLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3N3</accession>
    <name>A0A1E3P3N3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Heat shock protein SSC1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_68486</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039125.1">
        <property type="nucleotide sequence ID" value="XM_019184120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3N3"/>
    <dbReference type="STRING" id="683960.A0A1E3P3N3"/>
    <dbReference type="GeneID" id="30201366"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11733">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000014">
        <property type="entry name" value="Chaperone protein DnaK HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000003">
        <property type="entry name" value="Heat shock protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000007">
        <property type="entry name" value="Heat shock protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000004">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000003">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="605"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="613"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="649" mass="70449" checksum="21A317791AD11B1E" modified="2017-01-18" version="1">MLANSFMRNSLRNTTRRLPTLSRFNSNGKVQGQVIGIDLGTTNSAVALMEGKVPKIIENAEGARTTPSVVAFTKEGERLVGIPAKRQAVVNPENTLFATKRLIGRRFEDVEVQRDIKQVPYKIVKHSNGDAWLEARGQSYSPAQIGGFVLNKMKETAEAYLGKSIKNAVVTVPAYFNDSQRQATKDAGQIVGLNVLRVVNEPTAAALAYGLEKAETKVVAVFDLGGGTFDISILDIDNGVFEVKSTNGDTHLGGEDFDIVLVRHIVEQFKKENGIDLGNDRMAIQRIREASEKAKIELSSTVSTEVNLPFITADASGPKHINLKISRSQFEGLVDSLIKRTVDPVKKALKDANLTTSDVGEVILVGGMTRMPKVVETVKSLFNKEPSKAVNPDEAVAIGAAIQGAVLAGEVTDVLLLDVTPLSLGIETLGGVFTRLIPRNTTIPTKKSQIFSTAAEGQTSVEIRVFQGERELVRDNKLIGNFTLSGIPPAPKGVPQIEVSFDIDADGIINVSARDKASNKDASITVAGSSGLSDSEIEKMVNDAEKFAEQDKAKREAVENANRADQAVTDTEKSLKEFEGKVDQAKADEVQKQITTLRELIARTQAGEEVDAEELKTKTDELRQSSMKVFEDLYKNQGEGESKPEDKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3N5</accession>
    <name>A0A1E3P3N5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">FACT complex subunit POB3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_91746</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the SSRP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019186316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3N5"/>
    <dbReference type="STRING" id="683960.A0A1E3P3N5"/>
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    <dbReference type="OrthoDB" id="498543at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:histone binding"/>
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        <property type="term" value="F:nucleosome binding"/>
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        <property type="term" value="P:DNA repair"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
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        <property type="term" value="P:DNA-templated DNA replication"/>
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        <property type="term" value="P:nucleosome organization"/>
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        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="GO" id="GO:0031508">
        <property type="term" value="P:pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045899">
        <property type="term" value="P:positive regulation of RNA polymerase II transcription preinitiation complex assembly"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13230">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13231">
        <property type="entry name" value="PH2_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13229">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000001">
        <property type="entry name" value="Fact complex subunit ssrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000098">
        <property type="entry name" value="Fact complex subunit ssrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.220">
        <property type="entry name" value="Structure-specific recognition protein (SSRP1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050454">
        <property type="entry name" value="RTT106/SSRP1_HistChap/FACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048993">
        <property type="entry name" value="SSRP1-like_PH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000969">
        <property type="entry name" value="SSRP1/POB3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035417">
        <property type="entry name" value="SSRP1/POB3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024954">
        <property type="entry name" value="SSRP1_DD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038167">
        <property type="entry name" value="SSRP1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849:SF1">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21103">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17292">
        <property type="entry name" value="POB3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03531">
        <property type="entry name" value="SSrecog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00887">
        <property type="entry name" value="SSRCOGNITION"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="12">
        <location>
            <begin position="357"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="462"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="480"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="525" mass="59421" checksum="0E82F8F3EE34503D" modified="2017-01-18" version="1">MSNLDFERIYLNQSKANGRFRIAESGLGWKAASTGGSANKTQPFLLPSDELSSAQWSRGSRGWELRIQTKNKGVAKLDGFDQQDFNQLKNELQRLFNIQLDHKEHSLRGWNWGNTDLARNELVFNVSGKPAFEISYNQINNSNLAAKNEVAIDMNLINENNDTAGDELVELRFYIPGTVPYEDDDVAEEDREEKTSAAAFYDQLKEKADIGQVAGEALASFTDILFLTPRGRFDIDLYPSYLRLRGKTYDHKIQYRQVERIFSLPKPDDIHHLMVIHVNPPLRQGQTPYPYLVLQFAKDEELEVEINVSEEEFKEKYEGRLKKRYDQQTHLVLSHVFRGLTERRIIIPGSFTSKHGQAAVSCSLKVNEGYLYLLEKCFLFVTKPTVYVPYNDVQSVSISRAGDSSTSNRTFDLEVTLRSTSASHTFANISKDEQSTLESFLKEKGLRVKNDDIEQQARINAVLANEMDSSDDDVRMGSASDDESPDEDFQGESDDSDSDLAEEFDSDAPGSDDEAEGPPSKKPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3N7</accession>
    <name>A0A1E3P3N7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U3 small nucleolar RNA-associated protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_85038</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019185829.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="30203075"/>
    <dbReference type="OrthoDB" id="277439at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006709">
        <property type="entry name" value="SSU_processome_Utp14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14150">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14150:SF12">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 14 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04615">
        <property type="entry name" value="Utp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="133"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="398"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="585"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="46"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="149"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="409"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="437"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="464"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="591"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="623"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="638"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ59497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="850" mass="98099" checksum="592A3151FC5C4A69" modified="2017-01-18" version="1">MSKRMQKMAQNAFEIAQQQERARNGDSDSSDDDGAIINPRKRFGNEDGEDNNSNSEFEDEELDSDEALGSDDDFDILNTKFSQTIRDKQKKIKKGEIEEGDDDEDEGCQSVDESQFLPLSAVWDLDDKDLIDSKDTKDVILDDNWQSESESEDETSDESEDEEEEFPFENQSGDEDEDEVELTNLKKQLTKKDKRTKSQYQDLHIQENELALPGGGNEITLADMLSVVDNDASKKAMLIDKEETEESGKSLAIPLPKRIQDRHERKAAYEISKDEVNKWKETVKQNRESEVLSFPMNKQIEHNAPSTFQETPALTDLEQKVNSVLNQSSLLDDKKESTFEEIATAKMSVEELRKRQNELRKMRELMFRNEREAKRIKKIKSRSYRRIKKKEMLKNKSLIEGEESDDDEHDSKRAQERMSLKHKNNSAWSRSMIKSGLSKDQEAREEMEEMLRRGESLKSKILDRDEDEDEFGDSNDLNLDKDVDYDSETEARENLGKTGVLNMAFMKNAEARERTRNEETKNNLRRLHENEDIEEFDESERNNAINVHINPGRRIYTPGSGKSNAEMKELSQTVLDEDEIDNSKNLTARLSKSDKKQDSLLNREAEKQKISTKENADEDESNPWLTVSNNVQKSRKIHVTDKESSKLEKNESKMSKQRKRKAKEIEEPEEIIDVNETLKIVDPHGDDSDAGSDVDASKIHMFKQQDLIKQAFAGDDVVEEFEAEKRQVIRDEGDQVEDLTLPGWGDWAGGKNKKPKKNKIVKRTKGVDFDKRKDKNLKNVIINEKVNKKNAKYQSSAVPFPFESREQYERSLRMPIGQEWTSRETHQRLTMPKVTIKSGVVIDPLKAPFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3R9</accession>
    <name>A0A1E3P3R9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ornithine carbamoyltransferase</fullName>
            <ecNumber evidence="3">2.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Ornithine transcarbamylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_29613</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="3"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039200.1">
        <property type="nucleotide sequence ID" value="XM_019181631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3R9"/>
    <dbReference type="STRING" id="683960.A0A1E3P3R9"/>
    <dbReference type="GeneID" id="30198877"/>
    <dbReference type="OrthoDB" id="10252326at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903269">
        <property type="term" value="C:ornithine carbamoyltransferase inhibitor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019240">
        <property type="term" value="P:citrulline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000017">
        <property type="entry name" value="Ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000009">
        <property type="entry name" value="Ornithine carbamoyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001986">
        <property type="entry name" value="PRK00779.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF3">
        <property type="entry name" value="ORNITHINE TRANSCARBAMYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase carbamoyl-P binding" evidence="10">
        <location>
            <begin position="16"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase Asp/Orn-binding" evidence="9">
        <location>
            <begin position="168"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="331" mass="36729" checksum="809D7EAAAA5BE7AE" modified="2017-01-18" version="1">MFPKRYFSTTGAKSLRHLVSISDLTTKEFQTLVTKAETFKQLYKNGDSKSFVTNNQKLLGKTVALVFSKRSTRTRISSEGAAAFFGAQPMFLGKDDIQLGVNESFYDTTKVISSMVSAVFARVNSHSEIQQLAEHSSVPIINSLCDTYHPLQAIADLLTIKESFKETKGLKLAWIGDSNNVINDLAIASLKFGINVSIATPNGIEMDDKILKTAQKVSKETGATFQVSHDPKLAAQNANILVTDTFVSMGQESETIAKLKQFEGFQISEAHTEVANKNWRFMHCLPRHKEEVTDDVFYSKNSLVFEEAENRLYAAIAALEVFVVNNGDFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3V2</accession>
    <name>A0A1E3P3V2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_78788</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039378.1">
        <property type="nucleotide sequence ID" value="XM_019184788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3V2"/>
    <dbReference type="GeneID" id="30202034"/>
    <dbReference type="OrthoDB" id="5299849at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018815">
        <property type="entry name" value="Incr_loss_mito_DNA_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28029">
        <property type="entry name" value="PROTEIN ILM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28029:SF1">
        <property type="entry name" value="PROTEIN ILM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="121" mass="13415" checksum="E66877E3472489CC" modified="2017-01-18" version="1">MGNYTALVRIAGIAYLVYKLIYDSRELGRLISSYTGSKIIFTESESSLFAVLLAVIGVTDLVPYLEDNSDYFDSVVPIRFFAFFILAVVSYLGDFALLNSPLVLGYSILEAIVNGLMMIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3W8</accession>
    <name>A0A1E3P3W8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_62165</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038784.1">
        <property type="nucleotide sequence ID" value="XM_019183319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3W8"/>
    <dbReference type="STRING" id="683960.A0A1E3P3W8"/>
    <dbReference type="GeneID" id="30200565"/>
    <dbReference type="OrthoDB" id="24581at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042736">
        <property type="term" value="F:NADH kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.30:FF:000014">
        <property type="entry name" value="Mitochondrial NADH kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF26">
        <property type="entry name" value="NADH KINASE POS5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="420" mass="46860" checksum="79093C1DE0C05257" modified="2017-01-18" version="1">MSLRLLQGSLFRPSSKAQICHRLLSTSAPRYITEPIITPNKFVKIKPVTNLRSRSHPEFIQAHNSKLENLVWRNPLQNILIVKKPWSQHVRDSMVQFVTFLHDHYPEINVMVAKDVAEEISQDFNSYPKQTSSSPHILYTGEMEDIVPRTDLLVTLGGDGTILRGVSLFSNGRVPPVLSFSLGTLGFLLPFDFNHHEQAFREVYTSTAKVIHRTRLECHVIRNNSAPATNNQGMIHAMNDIVLHRGDTPNLTTLDIYIDGEFLTRTTADGVCLSTPTGSTAYSLSSGGSIVSPLVPAIMITPICPRSLSFRPLIVPLSSHIKIKIISRQNGENDVRFSIDGVPQDCLKVDDEIHVVNEIGTIFVDNVKIPGSTGQQRAIMKKKQIETGVYCVAKSENDWVRGINELLGFNSSFKNQAKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3Z6</accession>
    <name>A0A1E3P3Z6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_29937</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038829.1">
        <property type="nucleotide sequence ID" value="XM_019181668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3Z6"/>
    <dbReference type="STRING" id="683960.A0A1E3P3Z6"/>
    <dbReference type="GeneID" id="30198914"/>
    <dbReference type="OrthoDB" id="408373at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF33">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G14860)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="24"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="281" mass="31754" checksum="FEDBEF5E63CD9703" modified="2017-01-18" version="1">MLGRVNIGGAELEYLLEGEGLGDLIITLHGGRGFGSKESDHSVYKELALSGYRVLSFDFRGHGNSSFTPPFTFKRIVDDIEELRQHFTKDKVIIIGGSFGGFIAQQYAITYPNSLKALVLRGTAPSHHHEADAFKVLEKRLDKAPNVSVEMLRKVFSSFDSDEEFRLTIFAMLPLYSETYDANQGLKSALKTKYRAQSHNELYSEQEKYFDYREGLKQLNVPTLIIVGDKDWICPPKQSQLIHSLVKDSELLEVEGANHSAHLEKRALVLDKISSFLRDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P409</accession>
    <name>A0A1E3P409_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycolipid transfer protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_54012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039428.1">
        <property type="nucleotide sequence ID" value="XM_019182728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P409"/>
    <dbReference type="STRING" id="683960.A0A1E3P409"/>
    <dbReference type="GeneID" id="30199974"/>
    <dbReference type="OrthoDB" id="205255at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902387">
        <property type="term" value="F:ceramide 1-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902388">
        <property type="term" value="F:ceramide 1-phosphate transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3520.10:FF:000001">
        <property type="entry name" value="Pleckstrin domain-containing family A member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3520.10">
        <property type="entry name" value="Glycolipid transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036497">
        <property type="entry name" value="GLTP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014830">
        <property type="entry name" value="Glycolipid_transfer_prot_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10219">
        <property type="entry name" value="GLYCOLIPID TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10219:SF25">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY A MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08718">
        <property type="entry name" value="GLTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110004">
        <property type="entry name" value="Glycolipid transfer protein, GLTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glycolipid transfer protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="198" mass="22484" checksum="92242518F3F88139" modified="2017-01-18" version="1">MSTFFDEQKKSFVDVPVTDGKISTAEFLDASESLIKLFDLLGSTAFTVVKNDLQGNVTKVRTRLLETPDKSATLQDLVLNEIAEKKSKTATQGLLWLSRGLQFTAQAMSETVDNKDKELKKTFTDAYNKTLVKYHSFVVKPIFKLAMNACPYRKDFFEKLGQDQEKVLKQLIEWLEALESIVKIIFEFFESGNYGKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4L4</accession>
    <name>A0A1E3P4L4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cdc23 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_53346</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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            <taxon>Wickerhamomyces</taxon>
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    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ59822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:cyclin binding"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
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    </dbReference>
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        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04049">
        <property type="entry name" value="ANAPC8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TPR_16"/>
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        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cdc23" evidence="8">
        <location>
            <begin position="11"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="312"/>
            <end position="345"/>
        </location>
    </feature>
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        <location>
            <begin position="346"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="380"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="507" mass="59183" checksum="5899515C7EA2B976" modified="2017-01-18" version="1">MISIDHIPKYKLQVSKSEQDVFALAKTYFDCKEFDRCAHVLKNCKSQDVLFIRLYAMFLSGEKKKEEESEGVLGLHDHSSVNTSVSLILKELESILEQEPNGLKSPFLNWLHGVVLVKHKSMAAAKDAFIRSLAQYPYNWSCWAELLFCLPTFGEGMDVLSSLELQFQDNFAELIMLKFAKVVINQQCFQQTAELKTMLDSLIVKFPKFSFLKTQHALICYHALKYNIAEELFDEILVNDPLRLDEMDIYSNILYVMDKRSKLAFLAQFACAIDKFRPETCCIVANYYSMKFEHEKAIMYYRRALTLNRNSLSAWTLMGHEFVELKNTHAAIESYRRAVDTNYKDFKAWYGLGQAYEILDMHLYSLYYYQKAASLNPLDQRVWEALGNCYEKLDKIRDSITCYQKAINLNEVKNVSHLYKLALLYEKLQDPAKTYDYMFQCYQEEINEGVRNDETAKARLWLAKYETRNQNWEAAHKYAVDLTHGTGQEIEEARAVARETRNRINLQ</sequence>
</entry>
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    <accession>A0A1E3P4M6</accession>
    <name>A0A1E3P4M6_WICAA</name>
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            <fullName evidence="4">RING-type domain-containing protein</fullName>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4M6"/>
    <dbReference type="STRING" id="683960.A0A1E3P4M6"/>
    <dbReference type="GeneID" id="30200698"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="mRING-HC-C4C4_Asi1p-like"/>
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    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="56"/>
            <end position="75"/>
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    </feature>
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        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
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        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="249"/>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="ODQ60303.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="647" mass="73075" checksum="074CD46ADB148B2E" modified="2017-01-18" version="1">MSSTTLNNITTTEFNTSYINKSSQSWFVPRIFNSFTKAVEQQAKDAIPDRNATLSFGYLVSPYAGACMTMAIILNRTVVFASSRRNINRLSTVPKIALRIISIAVLIKALWDIFMQFTVITPDVRKFVGAFVSGEVPEVNIFLWRIFISLCISQFIETFVSITSNQSALNDTGLTLFEHSLAFQECQFLSIPSSQLLFICLFSIINQLVIHTLGIANLKKYQLIPSTVLGVSFLIFYCYNIYTGGLVFFPTVVIVSIIPQFLVFSIIFLCLLIYFLAVLVNQDSSGLTYTTMVRNLKSSINLQLNDDFHSALMRFGYIMFNAVEKEEYVNELSNLTLPSTTYLESNGHLVSGYLNNVQSNPELSSQQSQKVVDDDEFKNSNFARRMVNCAKLMGGIVRLVFKKKKKPTNDLPASISDQPKAFSNYEINSDITPSDYKLFLSNDSKSLLPENDSSEDFVYESESDIDPLDFESDIEDIDEIESPGYLSSAAMARLYDSGNLAQSIATESPTKQRSHSKAIDELIGSNDLLELITPKNIEQVSFLNSLRTHMIEDKRLTRSSYSEINQDELLREVILQNRTDHHNHSHHEEPQELACVVCQTNNREIIVWPCKCFALCENCRISLGVRGFSICVCCRRKVDGYSKIYIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4Q8</accession>
    <name>A0A1E3P4Q8_WICAA</name>
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        <recommendedName>
            <fullName evidence="3">Lactam utilization protein lamB</fullName>
        </recommendedName>
    </protein>
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        <name evidence="1" type="ORF">WICANDRAFT_30822</name>
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    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019181767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4Q8"/>
    <dbReference type="STRING" id="683960.A0A1E3P4Q8"/>
    <dbReference type="GeneID" id="30199013"/>
    <dbReference type="OrthoDB" id="5295431at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005501">
        <property type="entry name" value="LamB/YcsF/PxpA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30292:SF0">
        <property type="entry name" value="5-OXOPROLINASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30292">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YBGL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03746">
        <property type="entry name" value="LamB_YcsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="263" mass="29115" checksum="E3AD2A2B17A137A6" modified="2017-01-18" version="1">MSTSKLSGSAALNSKLLSKHEINCDMGEGFGRYQIAPDDEIMPFIDRANIACGFHAGDYNIMLKTVQTAKKYGVKCGSHPGLPDMIGFGRRPWSIPPEEIYKLILYQTGALKAFLDAEGVELSHVKPHGELYFYIERDEAVMRAALKAIKTFGVPVVGAKNAHYEKIAKEEGVDFIQELYVDCDYDAKGALVPPAKSRPKNPDIIHDAIYVAGSTDTITDQNDNKMDLHFEKNPISVCLHSDMPNSLNNVKKAREAIDKLNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4Y4</accession>
    <name>A0A1E3P4Y4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_83923</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039114.1">
        <property type="nucleotide sequence ID" value="XM_019185621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4Y4"/>
    <dbReference type="GeneID" id="30202867"/>
    <dbReference type="OrthoDB" id="3981238at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133658" evidence="2">
        <location>
            <begin position="20"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="406" mass="40961" checksum="40D1303D5B38E403" modified="2017-01-18" version="1">MVSARLLVTTLLSAALTSAQYFNSSSSISSFSSSSLSSEVSSSSSAGNSSSIVPSSSSSELPPSSSSSTPEETPFITGSFVDGVLQWEIFVPGSLGPWTQVSIAVSGQDYTVESVDVLVDGEATQADVATDGPSISASSGEQAPIGSYLSFLYRASRNSNARQFTTDAILTITTPDAVKLVRRAVITYEFSSTITVPDDASSLSSSSVTSGTTVPTSSGSTSEEVTTTIWGTSVVTTTKEGTVTSYTTYCPIATETCVLSTVTVSVESCPGVTKCHVGTTTAPGGGEHTFTYTESVVTITKGPGHGPGGEETTKVPGAPVTTITLGGESGKPTTGGAVKTSTNEEGSTVVVVYETSKVHGGEGSTGAPTGAPTGGSTTPAISTFEAAGHKLASGLGLLLPAFVFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P590</accession>
    <name>A0A1E3P590_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase family 20 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_89759</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039802.1">
        <property type="nucleotide sequence ID" value="XM_019186190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P590"/>
    <dbReference type="STRING" id="683960.A0A1E3P590"/>
    <dbReference type="GeneID" id="30203436"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005946">
        <property type="term" value="C:alpha,alpha-trehalose-phosphate synthase complex (UDP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000036">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase subunit Tps2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1020:FF:000002">
        <property type="entry name" value="Trehalose-6-phosphate synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1020">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase related protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF123">
        <property type="entry name" value="TREHALOSE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="712" mass="80450" checksum="81C6C6EB0F56EE5E" modified="2017-01-18" version="1">MGSASDGKEESQWWYDYVKFNEAYATKIVQTYKEGDIIWIHDYYLLLLPQLIRMHLPNAIVGFFLHCPFPSSEYFRALPKRQQLLDGLLGANKVTFQNYSFARHFISSCKRLLGSETTPNTVSVYGNTVSVDSLPIGIDVAKIEYDAFNKEIDTKVQAIKELYGHKKLIVGRDRLDSVRGVVQKLQAFEAFLEMYPEWRDKVILIQVSSPSYLHSQKIENQVSELIGQINGKFGSLNHTPVHHYQMRIDKSEYFALLRVADLGLITSVRDGMNTTALEYVVAQKFNHSPLILSEFSGTASVLKDALIVNPWDSVAVAKTIHEALNFDDVRKNHLQTNLSKDVVSIQNWTNQFLKSLLQGIEGDNKRKLTPALNRPLLLEHYKHSNRRLFLFDYDGTLTPIVKDPAAAIPSARLISILNKLSEDPKNQIWIISGRDQKFLEKWLGQNPHLGLSAEHGCFMKDINSSWVNLAASVDMSWQQKVEGIYTKFTDRTPGSNIEKKKVALTWHYRGADPELGEFQARELVKELEETIAKEYEVEVMTGKANVEVRPSFVNKGEIVKRLTLTRHGAPQKNGNVDDLNHKHEELPDFVLCVGDDATDEDMFKKLEAIEKDWEADEEFQKHAKFGNFGFYPVKVGPASLPTAATAHLSDPNQVLETLGLLVGQVSLFESAGTVELDDRGHIKNSEQSQRSEAAIVAARLKKTNSTSSRTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5U6</accession>
    <name>A0A1E3P5U6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_92436</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039424.1">
        <property type="nucleotide sequence ID" value="XM_019186361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5U6"/>
    <dbReference type="GeneID" id="30203607"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133674" evidence="3">
        <location>
            <begin position="20"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="67" mass="5918" checksum="C094DD421A995F04" modified="2017-01-18" version="1">MQLSQLVVFAATTFAVVSAQSSNSSSGGGGSSGSSGSGSIGSNAAGLNAAGAGAIGAAVAAGVALLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
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    <name>A0A1E3P628_WICAA</name>
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        <recommendedName>
            <fullName evidence="6">Protein RER1</fullName>
        </recommendedName>
    </protein>
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        <name evidence="8" type="ORF">WICANDRAFT_90178</name>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
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        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
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    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the retrieval of endoplasmic reticulum membrane proteins from the early Golgi compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the RER1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019186215.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3P628"/>
    <dbReference type="GeneID" id="30203461"/>
    <dbReference type="OrthoDB" id="448250at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:COPI-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN RER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10743:SF0">
        <property type="entry name" value="PROTEIN RER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03248">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AtER_Rer1p"/>
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    </dbReference>
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    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR016013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="183" mass="22048" checksum="BDEDFE06A175BE9D" modified="2017-01-18" version="1">MEDISLDESKINVINQNFNKFKVTYQRLLDQSTPHVKYRWLSFGFLLAVFLARIVFSQGWYIVCYGLSIYLLNLFLAFLQPKLDMTIEQTEQYENMEAGEMSQEFRPFIRRLPEFKFWHRATVATVLSLFLSLFPFTDVPVFWPILLIYFIILFTLTMRRQIQHMIKYRYLPFDFGKKKYGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6C1</accession>
    <name>A0A1E3P6C1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_83209</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat LST8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040120.1">
        <property type="nucleotide sequence ID" value="XM_019185505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6C1"/>
    <dbReference type="STRING" id="683960.A0A1E3P6C1"/>
    <dbReference type="GeneID" id="30202751"/>
    <dbReference type="OrthoDB" id="400at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030950">
        <property type="term" value="P:establishment or maintenance of actin cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031930">
        <property type="term" value="P:mitochondria-nucleus signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000339">
        <property type="entry name" value="WD-repeat protein pop3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037588">
        <property type="entry name" value="MLST8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842">
        <property type="entry name" value="G BETA-LIKE PROTEIN GBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842:SF0">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX SUBUNIT LST8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
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    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
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        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
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        <location>
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            <end position="112"/>
        </location>
    </feature>
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            <begin position="112"/>
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        </location>
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            <begin position="155"/>
            <end position="196"/>
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        <location>
            <begin position="246"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="303" mass="33902" checksum="D002F4DE409BDDA3" modified="2017-01-18" version="1">MSVILASAGYDHTIRFWEALTGVCSRTIQHADSQVNRLEITSDKRFLAAAGHLHVRLYDIRTTNPNPVTSFEGHTNNVTSIAFQSDNKWMVSSSEDGTVKVWDVRAPSVQRNYKHNCPVNEVVIHPNQGELISCDQDGNVRIWDLGENQCTHQLIPEDDVPVHSVSVASDGSMLVAGNNKGNCYVWKMSNQKDSTSLQPVTKFRSHSKYITRVLLSSDVKHLSTCSADHTARIWNVEDGFSLETTLQGHQRWVWDCAFSADSAYLVTACSDHYVRLWDLSTSETVRQYNGHHKGAVCVALNDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6H2</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ61026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="30203469"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="214" mass="24496" checksum="4F2B3604EFE0639E" modified="2017-01-18" version="1" fragment="single">MVLMKNLSQSTDDMIHPIEIRGSKIHLWNLPIQSFFAPEVKYLKISSAHFLPKVGRPIVTPNLEKMELIETSILECPELLTFPMTTKFKDCFFDAQMLQNDCYFEPFKNVKTLEILSCAGFKKMLGLEFPSLERLIIINDLGTVQISNIKAPKLGLLSVRSLEETVRIEDCQFGRLERFEFSGSAIDSCKKIWAPNLKVMEIAILFDNDDDDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6J8</accession>
    <name>A0A1E3P6J8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exosome complex component RRP45</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Ribosomal RNA-processing protein 45</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_67546</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040091.1">
        <property type="nucleotide sequence ID" value="XM_019184091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6J8"/>
    <dbReference type="STRING" id="683960.A0A1E3P6J8"/>
    <dbReference type="GeneID" id="30201337"/>
    <dbReference type="OrthoDB" id="10264038at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11368">
        <property type="entry name" value="RNase_PH_RRP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000005">
        <property type="entry name" value="Exosome complex component RRP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033100">
        <property type="entry name" value="Rrp45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF14">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0271">Exosome</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="11">
        <location>
            <begin position="32"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="12">
        <location>
            <begin position="190"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ60884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="304" mass="34016" checksum="D314AF0CD9F882DC" modified="2017-01-18" version="1">MAKGIEVSVNERDFVFEALKQGIRIDGRKLDQFRDVEITLGDEYGYVDVKMGKTRVVARISADIVSPFEDRPFEGLFQVSTEISPMASAMFENGRQSDDELLISRAIEKAVRRSGALDLESLCIIAGSKCWSVRADIHFLDCDGGFIDASCIAVMTGLQHFRKNDITVSGEQIIVHSTEEREPVELSILHVPICVTFAFFNPTDIEENIKGDSNSEISVVDATLQEELLKDGVLTVTINKNREICQITKAGGLPMDALTLMECSRKAYGIAEKLTKQIKELLEADYKKRNEGIQFRELRAENDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6R2</accession>
    <name>A0A1E3P6R2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_61136</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039774.1">
        <property type="nucleotide sequence ID" value="XM_019183115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6R2"/>
    <dbReference type="STRING" id="683960.A0A1E3P6R2"/>
    <dbReference type="GeneID" id="30200361"/>
    <dbReference type="OrthoDB" id="10255969at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43117">
        <property type="entry name" value="OSMOPROTECTANT IMPORT ATP-BINDING PROTEIN OSMV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43117:SF4">
        <property type="entry name" value="OSMOPROTECTANT IMPORT ATP-BINDING PROTEIN OSMV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="6"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="279"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="227"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="500" mass="56473" checksum="01EB8206DB602A9C" modified="2017-01-18" version="1">MSKPLIKLENALFKGLSAAKGSPTVFKNPISLEIKPEERWAVIGTFKTQLMKALASQYLPDPPLSRTYPFLNKSVWPNTVIQFLEFKGALPTAHLAARYEFFKDEFDETTRRFVVGNVNNSRIINEELVKDVFKKLKLEGLEDRWALGLSNGQTRRARLARALVREPKLLIVDDPFLGLDPTASSVVSDVLGNIPPNPHVVLGLRYQDVVPEWITHIAVVDENGLITTGAKNEVEEHLEELKAKDEELKKEQIALNNEKVENIKKLFSSKKVDYSVPLFEFKNISVAYRGQPVITDLSWKVLHGERWHVRGDNGTGKSTLLSLITAEHPQSWNSAIEMYGEPRRTGKQNFFDINAGIGFTSPELHAIFPQRLTAYEAIATGFVVGSFIPPKNLTQVEIERISAYFKEFNLESKKDTKFQDLSVSDQKLVLFIRAIIKNPDLVILDEALSVMDDARIEQCKGLLRHYPGTVLAIGHLESEVPDTDRYIRLLGPGKYEIELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P713</accession>
    <name>A0A1E3P713_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_83333</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL9 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040287.1">
        <property type="nucleotide sequence ID" value="XM_019185525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P713"/>
    <dbReference type="STRING" id="683960.A0A1E3P713"/>
    <dbReference type="GeneID" id="30202771"/>
    <dbReference type="OrthoDB" id="5555409at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.10">
        <property type="entry name" value="Ribosomal protein L9, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000244">
        <property type="entry name" value="Ribosomal_bL9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_bL9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020070">
        <property type="entry name" value="Ribosomal_bL9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036935">
        <property type="entry name" value="Ribosomal_bL9_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21368">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01281">
        <property type="entry name" value="Ribosomal_L9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="L9 N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein L9" evidence="4">
        <location>
            <begin position="30"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="221" mass="24470" checksum="D22570E5EC91E2B1" modified="2017-01-18" version="1">MSSNLFKQIPFARVPLVRYSVASKRFNKAEVQLLKDFPSVGKRGQILKVSHSLMRNKLHPHNGACYIVPGQGPRIPIYKEPKKIIRVAPKPSKVGTPNAPAPKKISIDIEGLLLNIPKDNSKASSRIQPSKASYSLFNLSVNLGDLKFKVQEEDGSLKTPIDKSAIAAQIKKMVNISVPESDITLKIKRDIISEITKTGEYRLEIGTSEKATKKIFVDAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P778</accession>
    <name>A0A1E3P778_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear pore protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_28239</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the nucleoporin interacting component (NIC) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040430.1">
        <property type="nucleotide sequence ID" value="XM_019181464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P778"/>
    <dbReference type="STRING" id="683960.A0A1E3P778"/>
    <dbReference type="GeneID" id="30198710"/>
    <dbReference type="OrthoDB" id="1918363at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044612">
        <property type="term" value="C:nuclear pore linkers"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044615">
        <property type="term" value="C:nuclear pore nuclear basket"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007231">
        <property type="entry name" value="Nucleoporin_int_Nup93/Nic96"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11225:SF4">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11225">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP93 NUCLEOPORIN NUP93 DEAD EYE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04097">
        <property type="entry name" value="Nic96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="4" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="841" mass="95553" checksum="A07006684DEA78A6" modified="2017-01-18" version="1">MTKEAETTFAPVSNGKRLLNELLESSRNLPNRDSDLGSIQLGINELRKKAHELRKNKVEVQQGDHFTKAHYLLAGKVSIDDVASSIKLLESNNVLEPRSKTSTQQGDLDSYLRSKKDENILSAIEQSLSSAARDFDNFVNQNISLDWKQRRDEIRESFGIVLNRQTAHTDSKAAKSNGADSKSISWGKSLKLLEDNSSKLNVNANYLTRRKFERYAKVINELNNTRQSSPNIDNEDAEFDLANEFINIYQNGSDSKSRQLLESWQIIANMKKGSRKGIDSKGFKNQIVSKSKTFLELQFFEYADSIYRKKVSEKGLPTIINKIKSFIDSQKLNANGNLLMVNGTPIWALVFYLLRAGCQEEALNLVLEHQAVFKKVEQSFVPYFKAYVNSPDKRLPQPLVTKIQNEFNHHIKNSIDGDPYRYAVYKILGRCQLSRRNISNIALTIEDWLWIHLMLVREDSQVEYDPAYEKYSLADFQEIILSYGVNKFSTNYLQVLLLSGLYDQAGKYALEINEIDGVHLTIGLAAYGLLQQSTQDTVNSSKGSHFSKILGSYIQSFKLSDPRIAVEYLILICLFPDQTETCHEALRELVLETREFSILLGKVNAAGVRVPGKIEQRKSLIALENDFLNKITEQAAITADKEGRIYDGLLLYQLSEEYEIVVSIVNRLLGEFLSNTELEQSLVDGNSETNPILIAENFLSLYQDISVKNKEICKKLLRIAEIRQIYLEKDWDLCLKNIEELDILPVNANTDLGSTRLKAEELAYLNESLLRNVPNLLIITMTCVAHKNEQINNSAFNGSIKEEKLKELRLIAKNCVVFAGMVQYKMPRETFSLLIGLEALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P781</accession>
    <name>A0A1E3P781_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Superoxide dismutase</fullName>
            <ecNumber evidence="3 10">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_29175</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="3 10"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040498.1">
        <property type="nucleotide sequence ID" value="XM_019181582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P781"/>
    <dbReference type="STRING" id="683960.A0A1E3P781"/>
    <dbReference type="GeneID" id="30198828"/>
    <dbReference type="OrthoDB" id="239262at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.990:FF:000001">
        <property type="entry name" value="Superoxide dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.55.40.20:FF:000002">
        <property type="entry name" value="Superoxide dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050265">
        <property type="entry name" value="Fe/Mn_Superoxide_Dismutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019833">
        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11404">
        <property type="entry name" value="SUPEROXIDE DISMUTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11404:SF6">
        <property type="entry name" value="SUPEROXIDE DISMUTASE [MN], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000349">
        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase N-terminal" evidence="11">
        <location>
            <begin position="27"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="12">
        <location>
            <begin position="116"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000349-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ61291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="221" mass="24824" checksum="5F1F24B86F3A9092" modified="2017-01-18" version="1">MSFLSTTTRALRTQASRAPLLYRTKVTLPDLDYDYGALEPYISGQINELHHKKHHQTYVNGYNAAIEQHAEATEKGDVLKTIELTQHIKFHGGGFRNHSLFWKNLAPTSQGGGELPSGELASKIEEQYGSIDKLIGLTNSKLAGVQGSGWAFIAKNNENGAIEVVQTYNQDTLGSKYTPLIAIDAWEHAYYLEYQNVKADYFKAIWNVINWKEAERRFASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7A1</accession>
    <name>A0A1E3P7A1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Very-long-chain 3-oxoacyl-CoA reductase</fullName>
            <ecNumber evidence="12">1.1.1.330</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">3-ketoacyl-CoA reductase</fullName>
            <shortName evidence="12">3-ketoreductase</shortName>
            <shortName evidence="12">KAR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Microsomal beta-keto-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_28709</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the microsomal membrane bound fatty acid elongation system, which produces the 26-carbon very long-chain fatty acids (VLCFA) from palmitate. Catalyzes the reduction of the 3-ketoacyl-CoA intermediate that is formed in each cycle of fatty acid elongation. VLCFAs serve as precursors for ceramide and sphingolipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a very-long-chain (3R)-3-hydroxyacyl-CoA + NADP(+) = a very-long-chain 3-oxoacyl-CoA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85440"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="EC" id="1.1.1.330"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Endoplasmic reticulum membrane</location>
            <topology evidence="12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.330" evidence="12"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040470.1">
        <property type="nucleotide sequence ID" value="XM_019181529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7A1"/>
    <dbReference type="STRING" id="683960.A0A1E3P7A1"/>
    <dbReference type="GeneID" id="30198775"/>
    <dbReference type="OrthoDB" id="5545019at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045703">
        <property type="term" value="F:ketoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141040">
        <property type="term" value="F:very-long-chain 3-oxoacyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030497">
        <property type="term" value="P:fatty acid elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05356">
        <property type="entry name" value="17beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000317">
        <property type="entry name" value="Very-long-chain 3-oxoacyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03107">
        <property type="entry name" value="3_ketoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027533">
        <property type="entry name" value="3_ketoreductase_fungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43086:SF2">
        <property type="entry name" value="HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43086">
        <property type="entry name" value="VERY-LONG-CHAIN 3-OXOOACYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000126">
        <property type="entry name" value="11-beta-HSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 12" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0521">NADP</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ61263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="344" mass="37635" checksum="B07B7CBDFFD5A0E1" modified="2017-01-18" version="1">MSFFNEIQSLAGNCKILNVLLYAALSVGVLKITTSFLSFAAVVLDTLVLPPVNFKKYGAKSNKWAVVTGASDGIGKEYSLQLAKKGFNIFLISRTQSKLEVLATELETKYKVETKTLAIDFASDKPSNYELISQLTKNLPITILINNVGQSHSIPVPFVETEEDELRNIITINNTATLRATQIIAPIIADTVKSNKGVRGLILTMGSFGGLLPTPYLATYSGSKAFLQSWSAALAGELASSNIDVECVISYLVTSSMSKIRRASLSIPNPKTFVSSVLRSVGRRSGAQERYATSTPYWVHAFMHFGIESTVGVYSKLANTLNLKMHKDIRVRALRKAARQQKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7F6</accession>
    <name>A0A1E3P7F6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leo1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_61917</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040568.1">
        <property type="nucleotide sequence ID" value="XM_019183273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7F6"/>
    <dbReference type="STRING" id="683960.A0A1E3P7F6"/>
    <dbReference type="GeneID" id="30200519"/>
    <dbReference type="OrthoDB" id="20844at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990269">
        <property type="term" value="F:RNA polymerase II C-terminal domain phosphoserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001209">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090262">
        <property type="term" value="P:regulation of transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009302">
        <property type="term" value="P:sno(s)RNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007149">
        <property type="entry name" value="Leo1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23146">
        <property type="entry name" value="LEO1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23146:SF0">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN LEO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04004">
        <property type="entry name" value="Leo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="433" mass="50268" checksum="962B0E9783140E70" modified="2017-01-18" version="1">MDNKRKKFRIESDEEDDIPVNNDNNTEEQQQHEDEQPQSKPEDSAPSPQPGNTNEEEEEEGDMDNLFGDDDDEEEDKAIDSRPADENGIVEDEEDEDDFRRNDGMEFAVDETKPQRELRSINLDVVRHPPKFHHEDGKLYDAKIPNFLEVDPTRFEGKAYLEQLVEDSKTLTKDEIELNRLKAENTLRWRYAKDSNGELINESNAQVVEWSDGSLSLKLGDEYFDVIQSQLTDTFLATYSEESGLLTTDGIFTNSMKIVPTSTNSKIHKKLTTAIQARQQTKPGAQSVYVDKDPEEEARALERQQEQLIRERRRKELKERKENEKYDSDSTPRPGQRATRESYYAQPRIRDEYEEDAFMVDDDEDVEEDDEEDDLEAAERLRRVKNEGAAKYNNEDEDDDEEDGDEGEDEDDEDGTAKARKKRRVIESDEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7G0</accession>
    <name>A0A1E3P7G0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase M24 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_61721</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M24 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040361.1">
        <property type="nucleotide sequence ID" value="XM_019183236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7G0"/>
    <dbReference type="STRING" id="683960.A0A1E3P7G0"/>
    <dbReference type="GeneID" id="30200482"/>
    <dbReference type="OrthoDB" id="5876363at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01089">
        <property type="entry name" value="PA2G4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000029">
        <property type="entry name" value="Proliferation-associated 2G4, a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047113">
        <property type="entry name" value="PA2G4/ARX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804:SF11">
        <property type="entry name" value="PROLIFERATION-ASSOCIATED PROTEIN 2G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804">
        <property type="entry name" value="PROTEASE FAMILY M24 METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="2">
        <location>
            <begin position="24"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="385" mass="41950" checksum="1E790A9202F93DB8" modified="2017-01-18" version="1">MSTAATTTKTTEPDYSLNSSDVVEKYRTAGSISQKVLDHVKSLVKDGAKIWDIAQEGDALIEEETSKIYNSKKNKVESKGIAFPTSLSPNNIADHLSPINAEDAQSNLTLKTGDVVKVHLGTQIDGFASIVSETVVVGASAQEPVTGKKADLLTAAYKASEAALRTIKPGNKNWDVTKVVAQVAKEYGVVPLEGMLTHNQERNVLTGPKEIIINPNEEQKGQVDTFEFEEGQVFGLDILMSTGEGKVKASEARTTIYKLTGNNYQLKLKSSHLVLNEIKRKASHFPFSIKNLDDPTKGRTGLKENCNHGVMIAYDVYQEKEGEQIAQVYVTVALTKNGLVKLTSSPFDESVVKSEKVLGDDLNELLSKPLKVNKKKSKAAKEEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7H4</accession>
    <name>A0A1E3P7H4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GPI-anchor transamidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_83540</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C13 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040588.1">
        <property type="nucleotide sequence ID" value="XM_019185560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7H4"/>
    <dbReference type="STRING" id="683960.A0A1E3P7H4"/>
    <dbReference type="GeneID" id="30202806"/>
    <dbReference type="OrthoDB" id="192611at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003923">
        <property type="term" value="F:GPI-anchor transamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000003">
        <property type="entry name" value="GPI-anchor transamidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028361">
        <property type="entry name" value="GPI_transamidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001096">
        <property type="entry name" value="Peptidase_C13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48067">
        <property type="entry name" value="GPI-ANCHOR TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48067:SF1">
        <property type="entry name" value="GPI-ANCHOR TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01650">
        <property type="entry name" value="Peptidase_C13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500138">
        <property type="entry name" value="GPI8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019663">
        <property type="entry name" value="Legumain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00776">
        <property type="entry name" value="HEMOGLOBNASE"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027610328" description="GPI-anchor transamidase" evidence="6">
        <location>
            <begin position="24"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR019663-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="306" mass="35411" checksum="440615BF9C474AB5" modified="2017-01-18" version="1">MLIPFHSLIRLFLGISLISSVLAAEESTQNESSSRHTNNWAVLVSTSRFWFNYRHMANVLSMYRTVKRLGIPDSQIILMLSDDVACNPRNLFPGSVYNNMDRAIDLYGESIEVDYRGYEVTVENFIRLLTDRWSPDHPRSKRLLTDENSNIFIYMTGHGGNEFLKFQDAEEISAWDIADAFEQMHEKKRYNEIFFMIDTCQANTMYTKFYSPNILACGSSELDESSYSHHSDVEIGVAVIDRFTYFNLEFAEKIDKNSTLTLQDLFDYYSLEKIHSHAGVRTDLFKRDPKDVLITDFLGNVQSVMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7Q6</accession>
    <name>A0A1E3P7Q6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_78090</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040668.1">
        <property type="nucleotide sequence ID" value="XM_019184657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7Q6"/>
    <dbReference type="GeneID" id="30201903"/>
    <dbReference type="OrthoDB" id="5340910at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="365"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="312"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="315"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="439" mass="48648" checksum="BF8FE3FE4D04D4E7" modified="2017-01-18" version="1">MTTTIITELSTTITSTLTSTQTAIVKGVLDLSIQTSIPVNGDNGITFAGKAANPGTTVSTITSSSTTTSSASSSKPSSSHINFGNNLLTQGTSASNGSNPKIGMAIGIPVAIIASVLIIVAFYFYLRYRKNYAHSQDAKLQRFYDDKYNYRISPLNESTSPYNLKTVYQLKTQPGDQFYRSNFDNQSSTIVPTTHNSKVPSIKEIPQGYDNSTKWATPIHKWFNNMSKSAMSSRASLSTGIFSPKTPVLREFNLKKQSNDEFNEKSPILPSFPEKSYGGHDHSFHSIESSPNGSIKQQVVRADTTRREIILQHPSKLKNKKKSKPLPKVPQSSTESSIKSLAGLEQIVMERMIPTTPKVDFAKYKNDKVYMVIKSYERNLADELSIKVGEYIRILARHTDGWCLAEKCNQNGEVLYQDINNYINEGRGVIPELCLQGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7S7</accession>
    <name>A0A1E3P7S7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UNC-45/Cro1/She4 central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_104494</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040625.1">
        <property type="nucleotide sequence ID" value="XM_019180418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7S7"/>
    <dbReference type="STRING" id="683960.A0A1E3P7S7"/>
    <dbReference type="GeneID" id="30197664"/>
    <dbReference type="OrthoDB" id="5574718at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024660">
        <property type="entry name" value="UCS_central_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45994">
        <property type="entry name" value="FI21225P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45994:SF1">
        <property type="entry name" value="FI21225P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11701">
        <property type="entry name" value="UNC45-central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UNC-45/Cro1/She4 central" evidence="3">
        <location>
            <begin position="165"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="673" mass="76614" checksum="1B87717163F123BD" modified="2017-01-18" version="1">MSIQRLQELFEVEDVGTLDTNTIEKELEAGAKTSKDEFRSLALKNPDRAVELSKKCGKPLYLISLATEDEPLQPIFDIFKNIAKISKKEDELYFALDAISLYVTFNKIPFQQVEFFVGACVPCLVKSEVCWAAFSRFYARYSMLDPTGLYEAVEQQIDILNAREDYGPIFKIVGHAFVIKKDQGKKFYDLESVQDSIRNVNEFSSPTTIKEGLSLLSLLCSDEELRKEIAVKFISVLTHGIKSNDIDIQELSTLVIIKTWSFTQVELEKSISIDELYNTITKKFTKTTIEGLAYLSIKPDVRKRIRNDDEVVFDLTKLLDKSQNDSQEDLYGVLVVLANLTTIEEKSEVSELKKHAQKGLESNIEEDPLEIVEFQADLLERNILGLISGHEVSPNSMIQIVRLIFNLSTSKDNRPQILAQGGLKILLKFLNLSTFSEETKIIAYRAVSNLLLTTDPKTIFELRQAQYALFLFLKTEELPLKDQLQALISLTNASSIDNGGLPEDQWDLIDHFLNHENTLIRRSAIELVCNLVQHKDGVAAVYNFAKQSSKKRYDLLVQYTLLKDVKSQCSAVAALSFGVTFPFIAEEVVKDKTLLDNLFTILNEQCKEEDLLERVLFVLYYLVFNSEENTAIMERLATNSQFKKGIETVLRNVKRGTEPFEMALEILKIVKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7T1</accession>
    <name>A0A1E3P7T1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">TBP-associated factor 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_24652</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040672.1">
        <property type="nucleotide sequence ID" value="XM_019181045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7T1"/>
    <dbReference type="STRING" id="683960.A0A1E3P7T1"/>
    <dbReference type="GeneID" id="30198291"/>
    <dbReference type="OrthoDB" id="308861at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09839">
        <property type="entry name" value="M1_like_TAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000011">
        <property type="entry name" value="Transcription initiation factor TFIID subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057345">
        <property type="entry name" value="Ig-like_TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037813">
        <property type="entry name" value="TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057991">
        <property type="entry name" value="TPR_TAF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25316">
        <property type="entry name" value="TAF2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25577">
        <property type="entry name" value="TPR_TAF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 Ig-like" evidence="9">
        <location>
            <begin position="556"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 TPR repeats" evidence="10">
        <location>
            <begin position="735"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ61465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1137" mass="130206" checksum="3B25ACB987A0AE78" modified="2017-01-18" version="1" fragment="single">RNYKVAHQKISIDVDLENNAIHGSTELIVIPTEPILKQIQLDCRQLEVHDVFVNNRRAVFSYDDPLHSEYSDFVNHDSNPIFNDISQHHFYKNKFEKMYSGENTEELTISMPEKLKLSLHDPTTIHSLTPSYKDSPGSHKMSTATDAVYTPVNVKIEYSLKNPRTGLAFVGGKGSQISKSEWHAYTVNSDYGVSTSSWVPCVDSLWEKSTWELEISVPRTIKDIGVTRLIGSANTEPEDDEDGDESNDIVVVGGDFSNQKESAHPIDMAKKLVSYSIFNPTAAQHIGWSVGPYVQVPLINLQEEEEPELMEEKDTTAVPASIYCLPSQKDDVVNTTIFLYKAIDFYSREFGSFPFTSYALVFVSDIDADVCGFAGLSILSKRLVYAPNVIEPIYTSTYALSVALSEQWSGINVTPKTFNDSWVTIGIAHYMAQQFIKKLMGSNEYKYRIKKQADKVCDEDIGKRPLAEPFLQFPVSTHDFKFIELKAPIVLFILDRRMTKTDKSFGLSRVIPKIFLQAMSGDLANGCVGTNHFQHVCEKVNHNRLDSFFRQWVYGSGVPIFRVTQRFNKKRMFIEMGIRQVQHQETAKIHPSPESFITEARRDQEGAYAPSSPTLFTGPMTIRIHEADGTPYEHIVDLKEGFTKLDIQYNTKYKRLKRSQKQQKSDKERNEVDEEETVLLHCLGDILQSEQEIQDWDLTEWTKEEEEKMNNEAFEWIRVDADFEWICKIHINQPDYMYASQLQQDRDVEAQLESINYFQTANPNTLYSSILLRTLLDSRYYYGVRVEAALGIAKFAKEKSNWIGTKHLLTAFKRLFCFENSTIPVPNDFSDFASYFLKKAISEALSTIRDQNSDCPIAIKRFLLDMLKFNDNLNNPYSDSFYVGHLITCLVNSICANALNDSVSKLNDVDKDFLVDAISEISRHQKMDEWIPSYNSLVGVTAFKEKLKLARRGLFTIDPDDLFEHTRSNYYEDVRVTAFEGLFQSGGLKNSEILRYFFLLATFEKSPYIRYKLIRAFAHSFGTVIIDGSSIDLDEEELNHDIEIEKKTRETDTGMVVIDEGAEDQFNSRKDTFAKATLKGAVDLLRRDYSKFEPLRKELWNALRIPLLSVSEKRTLFDVVSVLVPAYDFLISKFQIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7U6</accession>
    <name>A0A1E3P7U6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_52339</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040501.1">
        <property type="nucleotide sequence ID" value="XM_019182686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7U6"/>
    <dbReference type="STRING" id="683960.A0A1E3P7U6"/>
    <dbReference type="GeneID" id="30199932"/>
    <dbReference type="OrthoDB" id="498204at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000237">
        <property type="entry name" value="FAD dependent oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF150">
        <property type="entry name" value="OXIDOREDUCTASE TDA3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="3">
        <location>
            <begin position="21"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="3">
        <location>
            <begin position="219"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="475" mass="51703" checksum="3639487241E98998" modified="2017-01-18" version="1">MSDYLTFSATPSGKAEGKTHIIIVGAGIIGVCTAYYLTKHPDFSPETHHITIIESKRVAGGASGKAGGLLALWAFPQQIVPLSFKLHEELAKEYNGEDEWGYRRLTTVSLEGNIQNLLNTDDDSSSSLDDPKKDTQHEIKTSSPPPKTESSEDDDSTTTKSTSVESLDLKNPPPKKRTVTKNSIKNEHDSSSTTDLPKDLNWIRSEVIQDWSVLGGRDSTAQVHPYKFTNYILKKAMETGAVDLILGKVDEIQFSEETGSATGITYIPTKTLGKPNEQHVSLKGDQIVLTMGPWTSKILPDCPISGLRAHSITIAPHNGTVSPYAIFTELKIGRAKYVSPEMYARKDEVYVCGEGDTLVDVPETTDDVEVIKEKCDQLFHYVGKLSPNLSKGHILKRQACYLPVLDVPSSSGPLIGETNVENLYLASGHSCWGINNAPGTGKIMAELLLEGESHSADISALDPSLYFDASVLVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P838</accession>
    <name>A0A1E3P838_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_102473</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040776.1">
        <property type="nucleotide sequence ID" value="XM_019180354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P838"/>
    <dbReference type="STRING" id="683960.A0A1E3P838"/>
    <dbReference type="GeneID" id="30197600"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17327">
        <property type="entry name" value="MFS_FEN2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000064">
        <property type="entry name" value="MFS allantoate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF1">
        <property type="entry name" value="ALLANTOATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="303"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="402"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="462"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="73"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ61569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="524" mass="59422" checksum="E6CB46049A0FDB07" modified="2017-01-18" version="1">MSEKKTDQNNINQVNSNSATGDYEDYELGINGPKIQKDNNADIALQYAQENDLDFVMDPAYEKKVLRKIDWMLIPLLGLLQSVQLLDKTTNSYSSIMGLREDLNMTSYQYSWVGSIFYFGYLFFEYPANILLQKFPLAKTVSTAVIIWGIILMCHAACQNAASFLVCRFLLGVFESFMNPAFMMLTSQWWKQKEHFMRSCLWFGFQGFGNWMGAGISHGLATYRGVDGHSFESWRLLHIITGVMTIFLGIVSILHIPDIPTKAWFLNEKDKKYVVERIRENQQGFGNKKYKKYQVLEAFRDPATYLAFIYAFTYATANGSFTNFGTILLNQDFGFSTTTSLLMGMPGGALDIIYSPFLAYVNYKFMNNRRLISCAITNCITIIGMSLLNFTDHRGSRLAGYYTFYITTAVMSGMLSIVSSNVAGHTKKSTVTTLFFVGYCVGNISGPQTFKGSEAPNYHSAKATLLACYIIGTICILGLFALYQHRNKIRDQKKAELGDKYIVPENVEFSDLTDKENLEFRYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8B4</accession>
    <name>A0A1E3P8B4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Class II aldolase/adducin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_61754</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040399.1">
        <property type="nucleotide sequence ID" value="XM_019183240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8B4"/>
    <dbReference type="STRING" id="683960.A0A1E3P8B4"/>
    <dbReference type="GeneID" id="30200486"/>
    <dbReference type="OrthoDB" id="3238794at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000009">
        <property type="entry name" value="Class II aldolase/adducin N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004855">
        <property type="entry name" value="PRK06208.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF25">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 14 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="1">
        <location>
            <begin position="65"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="298" mass="33268" checksum="35676C414E6B2438" modified="2017-01-18" version="1">MSATSTTESKQTATLKLGSKDDKAYLKKENYGEAGVHNIAQGEYEYAHIKPKFEDKHKERQWLLEHMAGVFRVFGRRGYNEGAAGHISVRDPVDPETFWINPLGRHFSLMRVSDLVHVDKEGKVIGGNKAAVNAAGFQIHSELHKARPDVHAACHTHSVYGKTYSTFGKELEMLNQDVCMFYKSHSVYTDFGGVVLEKEEGQRLAKALGNGKAIILQNHGLLTVGSTVDEAGYLFTLMERCCEVQLLADSVKGYEKKIIGDDEAAYTEHMTGDPETLYSEFQPDYELEVELTNGAFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8H1</accession>
    <name>A0A1E3P8H1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Iron transport multicopper oxidase FET3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">WICANDRAFT_91052</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040721.1">
        <property type="nucleotide sequence ID" value="XM_019186274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8H1"/>
    <dbReference type="STRING" id="683960.A0A1E3P8H1"/>
    <dbReference type="GeneID" id="30203520"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901684">
        <property type="term" value="P:arsenate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010106">
        <property type="term" value="P:cellular response to iron ion starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033215">
        <property type="term" value="P:reductive iron assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046688">
        <property type="term" value="P:response to copper ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13851">
        <property type="entry name" value="CuRO_1_Fet3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13877">
        <property type="entry name" value="CuRO_2_Fet3p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13899">
        <property type="entry name" value="CuRO_3_Fet3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000024">
        <property type="entry name" value="FET5p Multicopper oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000025">
        <property type="entry name" value="FET5p Multicopper oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044130">
        <property type="entry name" value="CuRO_2_Fet3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF361">
        <property type="entry name" value="IRON TRANSPORT MULTICOPPER OXIDASE FET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0186">Copper</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0410">Iron transport</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133726" description="Iron transport multicopper oxidase FET3" evidence="18">
        <location>
            <begin position="22"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="565"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="21">
        <location>
            <begin position="41"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="19">
        <location>
            <begin position="161"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="20">
        <location>
            <begin position="367"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="612"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="ODQ61514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="24">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="643" mass="71952" checksum="43D9133CDE5C0B0A" modified="2017-01-18" version="1">MVSLTNVLTSTILFASTLVAAETHVFNWTTGYGERDLDGTGNMRRIITCNGEFPWPDVQVQKGDTVQVYLTNGFDDKNTSLHFHGLFQPGTNQMDGPEMVTQCPIGPGQTFLYNFTVTEQHGSYWYHSHTSGQYMDGMRGLFIIHDPDYPYKQGKDYDQELAITLNEWYHKTSYELLPTFLNKFNPTGAEPIPQNFLMNNTRNVTWEVQPDTTYLVRLINIGGFVSYYFYIEDHELEVIEVDGVPVEKNSTNMIYISVAQRYTFLLRTKSDTSKNYAIMQLCDVGMLDLQPADLMVNATNTLSYSSSNEEVSEVTIDDYDDHLDDFYLTPVNKTELYDEPDLTITLEVVMDNLKNGINYAFFNNLTYTAPKVPTLMTVLAAGDLATNAEIYGSNTHAYVLGKNDIVEIIINNNDTGRHPFHLHGHVFQLIDRDTYSGTEDATDIVPYNADDHPEFPHYPMMRDTVHVNGHSNMVLRFKADNPGVWFFHCHVDWHLEQGLGLILIESPADIQNNPSQQLTENHKAVCENLNISINANAAANTNFLDLTGENVQAPAVPTGFTAKGYVAFVFSCVAGVLGLITISIYGLIDISNVEEKVARELDVDIHKLAAEEDDSLAGSNEKNGASHGVGVVDDNSSDSAPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8R3</accession>
    <name>A0A1E3P8R3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Genetic interactor of prohibitin 5, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_75903</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Essential for respiratory growth and required for maintenance of mtDNA. Required for cell survival in the absence of prohibitins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GEP5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040907.1">
        <property type="nucleotide sequence ID" value="XM_019184285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8R3"/>
    <dbReference type="GeneID" id="30201531"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031455">
        <property type="entry name" value="Gep5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17053">
        <property type="entry name" value="GEP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="301" mass="35209" checksum="0D05F495412160BF" modified="2017-01-18" version="1">MKAPFKVPQGLSSTFWNKIQHLEIDTRTKDRLIKDAVKIGTKNPEDEKLLQLETMVDNVKNHQKKSLHPILARYYLDKGYSENRHINYFMKNHIDRWLLESLVTRYTNEKDSLYVQAKSNTRLNSLKLPREERQLPSVMELLTGNLQKNNEEKKFAIKSSELKTKGGIVINSRLSKNPKVVGKRTTNDAVEEAEEKEEILPLEFELPDNEQKLEQMDIMVRQVIKHMNFLSSHKYFHTRKLASPIVQTPATLLGEDIPEFRKKNLTQKKLTYIRGIFQLYPPIRIDDSNYLTSMVEVQKVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9E6</accession>
    <name>A0A1E3P9E6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_22463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041195.1">
        <property type="nucleotide sequence ID" value="XM_019180946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9E6"/>
    <dbReference type="GeneID" id="30198192"/>
    <dbReference type="OrthoDB" id="60822at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011893">
        <property type="entry name" value="Selenoprotein_Rdx-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02174">
        <property type="entry name" value="CXXU_selWTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36417">
        <property type="entry name" value="SELENOPROTEIN DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G05220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36417:SF2">
        <property type="entry name" value="SELENOPROTEIN DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G05220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10262">
        <property type="entry name" value="Rdx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="92" mass="10643" checksum="37D1564E8CF6609A" modified="2017-01-18" version="1" fragment="single">VTIKFCTKCKWNLRAAWYLQELLSTFGDKLGEVALIPYESGVFRVEVQKDTNSPSVQVWDRKENDGFPDSKILKQKIRNEVFPDEVLGHVDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9M0</accession>
    <name>A0A1E3P9M0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_24730</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041298.1">
        <property type="nucleotide sequence ID" value="XM_019181050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9M0"/>
    <dbReference type="STRING" id="683960.A0A1E3P9M0"/>
    <dbReference type="GeneID" id="30198296"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033567">
        <property type="term" value="P:DNA replication, Okazaki fragment processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000146">
        <property type="entry name" value="Ubiquitin-specific protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF758">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="38"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="394"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="444"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ62091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="493" mass="55434" checksum="DE18AFE44A154301" modified="2017-01-18" version="1">MDVTDFYGIEESPADRGSNGSKRLVKNWGAQFSTQKPLGLLNHGVTCYTNAAVQAMIHIPAVQHYLVDVLQGKYKDTINPRSVSQVLAETSARMWGLEKIAHKKGGPKFINPKKLIGRLDDINCMMSEWQQEDSHEYFMSLLSRLQEDSTPKGHKLNESIIYDIFGGSLDQLVTCKNCGHVSKTQQEFYDLSLHLGTSRKNSQTEPIDKEELSAIQQQQSLDEAESKHRFSINKSIRDFFSPELIKTDRSDKSGYVCEKCKKRTNAIKRSTIDRAPETLAVHLKRFRFNGNSSSKVKQGVSYPLILDLSQYTTRHEPVLYQLISVVVHEGRSVSSGHYVSHCRQPDGVWATYDDEYINIIPEKQALRDPSAYYLIYTRLTHKDVKQGSDVVKKATNMKPTPVPSSKAPTPKSSQPPKSLSPTTKKMKSLRAGIITPSVPDPSLVQKSPQAPTSRKQKFKGLKPTNNLGNLGGKQQKRYAKDSSGNASKRFRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9V4</accession>
    <name>A0A1E3P9V4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_59750</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040874.1">
        <property type="nucleotide sequence ID" value="XM_019182867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9V4"/>
    <dbReference type="GeneID" id="30200113"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="13"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="163" mass="18359" checksum="B18AB8DFA3A66C7D" modified="2017-01-18" version="1">MSDQPPSYNEKNQPYNQPGYNPQPVQQQQDYHQPQPQQPIQEQQPQQSSQDQYKQSQQQSQGQGQGQGQPYVVVQHGNIPPEPKKFGGCAKFGLYLLCFIFPPISTLIVSPKGSDTIINILLLFLVGIGSIIHGIYIVYKYTSGEGSYDAWEEKYGKRKPTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA21</accession>
    <name>A0A1E3PA21_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_82180</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041271.1">
        <property type="nucleotide sequence ID" value="XM_019185344.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3PA21"/>
    <dbReference type="GeneID" id="30202590"/>
    <dbReference type="OrthoDB" id="6287725at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007118">
        <property type="term" value="P:budding cell apical bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025614">
        <property type="entry name" value="Cell_morpho_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025481">
        <property type="entry name" value="Cell_Morphogen_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039867">
        <property type="entry name" value="Furry/Tao3/Mor2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029473">
        <property type="entry name" value="MOR2-PAG1_mid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295">
        <property type="entry name" value="FURRY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295:SF30">
        <property type="entry name" value="PROTEIN FURRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14225">
        <property type="entry name" value="MOR2-PAG1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14228">
        <property type="entry name" value="MOR2-PAG1_mid"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14222">
        <property type="entry name" value="MOR2-PAG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cell morphogenesis protein N-terminal" evidence="2">
        <location>
            <begin position="195"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="762"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1232"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1531"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis protein C-terminal" evidence="3">
        <location>
            <begin position="1733"/>
            <end position="1983"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2166"/>
            <end position="2221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2233"/>
            <end position="2255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2166"/>
            <end position="2184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2206"/>
            <end position="2220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="2277" mass="257993" checksum="21A09AFE3B6747B0" modified="2017-01-18" version="1">MNATTTTPTQKESFQSRRLTSNNATIDEINTIRKRLATDSKTPTEYTLHILFTQFVRHAERKLNVCLTYPLNTEPPIVDLLAEGVDPQFDKIIASLGYISKNNPKPVIDSVMFWRKSKSEVAAMAADAAEKAIIAAKEHKESTTFRKHTHSSSSVSRFSHKRNNSSKSTVNVTDSRSGYLEQQVQSSRETAIQGDRKSLISIYILCRVLIEVVKQLNSDSTGEDLGDKLEEIVFSQLRSTDPILISSSIIRSANWNLFAELLGYMSDKRFLSVSDRFIADLEKIPAVVPKDIEPSIHLLIHGMRYLKLKNYPLEDFEESADFIQSIAKFFSKTKSHSIKVAYSQVLSHILLPLAESLTAEVNHPTWAEAVNTIYKTCETIELNPKSWLTNLTLITSVLCVSPSDLFLSQWYDLVESNIRNLKSKHATLEERVTITTCIARLIWVYIFRCTETLNNTTRKIESIMKSMFLGKGKQSWLVSDASLILPCVQIFRAIGFAAYNQTVENFVIPLLRSAFSGHSLDSVQHEKVVLAVKAYTAMLADKERPNFPEDNDSIAQPDVDIDIKSGNHDEICKYVDSLLFLLDSSIGASYDQQSEQQQTPSTPFYYSTKSPFSSFRFGSEMSVQLSKSLHIELFAVVLESVPWCASGPSRKTVELLCRNSLNSDFRVSSAASSSLKILASRKSPNGLITSFAKFAFNFSERSGYSQSHVISKELLRLYLDLLQCWLSSIQKRKEEDGDGMDDCENKIVDELQWKNTITVIEEVEGHGLFFLASQDFEIRKLGLDILRITESFDQALYEQTKKTKDTHSRSHSKFAADIGTRVIFILQTTDFFKLISSQKQYLSVAERSRLSKLNQKQRRDVLIRLAESDYGVDSALWFRVFPKFLNVCFERCPITMALCRSIVCTRLVQIHEFILDFAETPRTSQKLTYGLPEVLIEQWKLYLIVACTSLTSTSEQRLHIPANYSHGKKKSQQLLTIQHSTITSARSVFKLTLNLLNGYNQAVRDAIITGLSCMNINIFRTFIECVEPIFAQWEQELGKQIHSDDSKLRIEVTHVLNIVSRFMEEDVIAQDEWIVDKLVSFIKIIKSFLSQSEVQHSFEFQKLRRYFCGLLENVYNGIQKTGNSGKWLPFEARVACFTYLEEWCGYGKYSAISKQRYAQMSKHVTNSQEHAALNSAIEFERQILSIAATNAMVALLNGKIHELRGQTMLKFDLPGLLSWIDLLFGSSNERIYSLAKKALHSLFKFNTSDTELYHEVFKKCIFDQKISIIENYYVIITEALLEANEYPIKRHQVLLLGFYGCGCNNYEIRTAAMKLLIDAEKKLFGTELVNSFTERVMNKNKTIYKRASLDISAYFASSYPEENIFLISELTMYFHIVSFESRRDILTVLIPWLQTVELHVADDDIDIQSYMVLSNLFEITIRFSDSIPNEIEALWISLGNGANFANIDAILRFSVRASLLKRNPLFVEFASKVLVYLSGTSLNLADSLVSFIEPKAMVPPQISNLELPTNDIGLPHVANIWKTLNYSGKDSVFSLGQLSLIFLSDLLVIPKESLTPKIPLLLHTSFVLLDHYLPVVQESAAKVIIYLLSTVNSHSKLSETKALIRKNHKSLWIYDDLNHDKSGARSPKDMDLLVRNTLEIFGATYPNIQQEWGRVALSWATTCAVRHIACRSFQVFRSLLSFLDQSMLKDMLHRLSNTISDETHDIQGFAMQILMTLNAITSELDAEKLIDFPQLFWVTVASLNTINEHEYVELLSVLSKFLSKIDLDSEDTVSCLVSTFPSNWEGKFEGLQQIIMIGLRSSVSLDASLQLLDRLNKLKDSEVIAGESRLLFALLANIPRFSHALEEAELTPQILESAKTLSEMALAKNQPNLARIIDSFAKNRFRSKNDFLSQTINFISENFLGEYSGQTVIFLLGLLSNKIDWVKIETMKVLKLLFPKIDLSEPQFQGCGADLISPLLRLLLTPYAQQALEVLDETTSISGSKLDKDILRMSLGNKTIRKDYEKTATLFGIPEETGWSIPMPAITAATTRNNVHAVFSTCRTVVESEIPETEQQQEQIKFHMEDYAYGQSSSNNEFQDSASVAEENDGSLSHMWAELDNLDSFFTKDNFSENTSNNGAGHHFRSTSVDTRYSNNDQLAPYESAPQIYDKKVSVILNRSLARTQSNTSFKTSLADSFGNSPISENRHLSGISSREALMRSRGEMSSGPNSGSSTPTSPNFSKEDLFRFEGLLAQPPPKPKKRLSNNKQQQKQWPILRMQHKLDIQLYNEELLKLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA81</accession>
    <name>A0A1E3PA81_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Pyruvate decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_25906</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041508.1">
        <property type="nucleotide sequence ID" value="XM_019181203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA81"/>
    <dbReference type="STRING" id="683960.A0A1E3PA81"/>
    <dbReference type="GeneID" id="30198449"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050177">
        <property type="term" value="F:phenylpyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004737">
        <property type="term" value="F:pyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000949">
        <property type="term" value="P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000950">
        <property type="term" value="P:branched-chain amino acid catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000951">
        <property type="term" value="P:L-methionine catabolic process to 3-methylthiopropanol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006569">
        <property type="term" value="P:L-tryptophan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02005">
        <property type="entry name" value="TPP_PDC_IPDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07038">
        <property type="entry name" value="TPP_PYR_PDC_IPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000019">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000024">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047214">
        <property type="entry name" value="TPP_PDC_IPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047213">
        <property type="entry name" value="TPP_PYR_PDC_IPDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF3">
        <property type="entry name" value="TRANSAMINATED AMINO ACID DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036565">
        <property type="entry name" value="Pyruvt_ip_decrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="13">
        <location>
            <begin position="28"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="11">
        <location>
            <begin position="236"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="12">
        <location>
            <begin position="434"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="479"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="506"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="508"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ62301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="599" mass="67627" checksum="03149F49D3711601" modified="2017-01-18" version="1">MTPVSLEPNQQSTTTTITTSNSSQLPFGEYLFRRLEQVKNVKSIFGVPGDFNLGLLEHLYKTQLNWIGGCNELNSGYAADGYSRYTNSLGVLITTYGVGELSALNAIAGGFAEYSKILHIVGIPKTSVLNNEVEKAKHLHHLVPNIDSLKRSDHYVYTKMVKDVSCISKVLDDSKTIVEDLDQVIQAIFKTSRPGYIFLPLDMADALVDASKLSQTPADYHLIKDNHPLKTEEITQKILELVYKSKNPSILADVLVSRYHNATELLREFVDLTQFNNYTTFMGKSILDETNPNFVGDYIGLESNIGIKENMEASDLILYFGSYINEINTGHYSFDFSEDQLILLHPDYIKIGSEFYHGVNFVNVLSSLLIHLDSSKVPTNPPLQYEIKLRFPYLPSKASISQSHMLEKFESFVQPNDVIVCETGSFMFGIPDLKFKSNVKYIAQGFYLSIGMALPCSTGVGIAMKDQNSSSRLILVEGDGSAQMTIQEISSFVHYGLNPIIFLLNNSGYTVERIIKGETRDYNDIKQWNWSKAFEFFEFGDEKNSEVKIVSNEQELTKAFASTELNPTKLRFHEVILDKMDCPWRFHHMNGYRHLPATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA90</accession>
    <name>A0A1E3PA90_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_23980</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ING family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041530.1">
        <property type="nucleotide sequence ID" value="XM_019181009.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA90"/>
    <dbReference type="STRING" id="683960.A0A1E3PA90"/>
    <dbReference type="GeneID" id="30198255"/>
    <dbReference type="OrthoDB" id="5411773at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15505">
        <property type="entry name" value="PHD_ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059153">
        <property type="entry name" value="NSD_PHD-1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23011">
        <property type="entry name" value="PHD-1st_NSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="10">
        <location>
            <begin position="7"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="7">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="7">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="7">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="7">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="60" mass="7195" checksum="A8DD09B3EAB5949A" modified="2017-01-18" version="1" fragment="single">DEDNDNHLYCICRRTSFGEMIGCDNPKCKYEWFHYSCVGLTRAPRGRWNCPLCKNRKKHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAH6</accession>
    <name>A0A1E3PAH6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Splicing factor subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_60473</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the SF3B5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041622.1">
        <property type="nucleotide sequence ID" value="XM_019182999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAH6"/>
    <dbReference type="STRING" id="683960.A0A1E3PAH6"/>
    <dbReference type="GeneID" id="30200245"/>
    <dbReference type="OrthoDB" id="274726at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009846">
        <property type="entry name" value="SF3b5/RDS3-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017089">
        <property type="entry name" value="Splicing_factor_3B_subunit_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20978">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20978:SF0">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07189">
        <property type="entry name" value="SF3b10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037010">
        <property type="entry name" value="Splicing_factor_3B_subunit_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR037010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="86" mass="9799" checksum="0DC1DA0AD8F021F9" modified="2017-01-18" version="1">MVSADKVREQQVYDQLKSRHIGLGDSNTTKHEWMTNVHRDSYASVVGHPALLEYLAIAHDKPKVQLKEELIMKMVKPCAPITKSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB41</accession>
    <name>A0A1E3PB41_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_60228</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041369.1">
        <property type="nucleotide sequence ID" value="XM_019182957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB41"/>
    <dbReference type="GeneID" id="30200203"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="309" mass="34944" checksum="1993FEAAEA560557" modified="2017-01-18" version="1">MVLISTQSRFIEMRVSRQLAKQLTNVRKLYGSSAAKSAYGSFDKKAHDSSASDFDESKHFLTYFKTGGAKVYSQAELIKAVNEITGTVIKPFNSAAEAQEHLASNPPQVKSEILQRVLDLNQEDVIRQQRESGVVKLYTNAFNKRVSQYSFTGYSVFAEDNSIVISESIEKKTKKSLEKAHLEAVYHASLAIYDQIMDLKTGEPPTAVGKYEIWTSAPQIHLYLSRVEKNEEKWMKSLEPEDLESLKRLLFIRDFYKANADLFEGFEFKIVSIGDMDLRSDKMFIESKRLAVDGAADGAPGNLRHKVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBC2</accession>
    <name>A0A1E3PBC2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Ornithine aminotransferase</fullName>
            <ecNumber evidence="4 9">2.6.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_66945</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 2-oxocarboxylate + L-ornithine = L-glutamate 5-semialdehyde + an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:13877"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="EC" id="2.6.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.13" evidence="4 9"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041920.1">
        <property type="nucleotide sequence ID" value="XM_019184066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBC2"/>
    <dbReference type="SMR" id="A0A1E3PBC2"/>
    <dbReference type="STRING" id="683960.A0A1E3PBC2"/>
    <dbReference type="GeneID" id="30201312"/>
    <dbReference type="OrthoDB" id="10261433at2759"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00358"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004587">
        <property type="term" value="F:ornithine aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019544">
        <property type="term" value="P:L-arginine catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010121">
        <property type="term" value="P:L-arginine catabolic process to proline via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006591">
        <property type="term" value="P:ornithine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000011">
        <property type="entry name" value="Ornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000152">
        <property type="entry name" value="Ornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010164">
        <property type="entry name" value="Orn_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01885">
        <property type="entry name" value="Orn_aminotrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF18">
        <property type="entry name" value="ORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ62713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="430" mass="46872" checksum="F89F9AF03E85887C" modified="2017-01-18" version="1">MNFNISSKEAQQLEEEYSAHNYHPLPVVFHKAKGAHVWDPEGNEYLDFLSAYSAVNQGHCHPKIIQALIDQAQNLTLSSRAFSSDVFGLFAKYVTEFFNYEMVLPMNTGAEAVETGLKLARRWGYVKKGIKPDEAIILAAENNFHGRTLGIISMSTDPDATTQFGPFLPKTGPLIPGTNKTLRYGVIEDVELAFKNAGDKIAAILLEPIQGEAGIVVPPPGYLERVSQLCKEYNVLFICDEIQTGIARTGKLLCYEHSQNVKPDIVLLGKAISGGVLPVSAVLSSKEIMLTLEPGSHGSTYGGNPLSSRVAIAALEVVKDEKLVERSAELGEFLKQELIKLQKESNGIISEVRGVGLLSAIVIDSSKANGRTAWDLCLLMKDHGVLAKPTHDHIIRLAPPLVISKEDLLKGVESIRSSLVKLPTVEKASH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBZ7</accession>
    <name>A0A1E3PBZ7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nucleolar pre-ribosomal-associated protein 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_12604</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042143.1">
        <property type="nucleotide sequence ID" value="XM_019180537.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3PBZ7"/>
    <dbReference type="GeneID" id="30197783"/>
    <dbReference type="OrthoDB" id="72892at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059018">
        <property type="entry name" value="HEAT_URB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039844">
        <property type="entry name" value="URB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032436">
        <property type="entry name" value="URB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021714">
        <property type="entry name" value="URB1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13500:SF0">
        <property type="entry name" value="NUCLEOLAR PRE-RIBOSOMAL-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13500">
        <property type="entry name" value="NUCLEOLAR PRERIBOSOMAL-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26140">
        <property type="entry name" value="HEAT_URB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16201">
        <property type="entry name" value="NopRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11707">
        <property type="entry name" value="Npa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="URB1 N-terminal" evidence="1">
        <location>
            <begin position="34"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="URB1 central HEAT repeat" evidence="3">
        <location>
            <begin position="535"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="domain" description="URB1 C-terminal" evidence="2">
        <location>
            <begin position="1376"/>
            <end position="1578"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1635"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1635" mass="185848" checksum="79B5B0DFBD0A3B59" modified="2017-01-18" version="1" fragment="single">SGSVDNGVLEKVGTLLKEESLNEISQFLSKGFGNQLLQSWSYFAQVNDHRKFTTSTISLSKLLGFISNNDELQEYGVQLIKDILTSYIKTIYRGLNSMRASLTNPIIRLLNEIVIFKNGALIDDFVALFDFTLPVLPKLLNPSKSELSNIEGTKEKEHLSMRYCFIRFLLNLLKYSAPLLRKDLLLSNSKIMINWFKHICTIDSDGLIGLTISVWDEKIIKEQSFKKATKIKVFNEWNLSKLLPIYYTKDKELRSVFDKFILSLATDSKYGLRFNDDEAWFLQTSGSGTISVNGKSFKTHNKLLYGLITNLKPWDDDLQLNTTIQILKASPEMIPPFMNYLASRGLHDPKLSSYWLGQTLLLSKVICLDIPADIESHDAMVPPSTNIMIELVVPCVLNRAVLARCLISDSFLIRQLSSQLIINSILKLEQIISLYSKKGWDEAKIQLLNKVRLSLPEISTVLNSLTDSYNKKSENKILLLTLLSVVNGYMKLFSESLNFSQQLSKPYSDIINEKQFKSIDFVLLDRFFQLQEGDSTQLKWWNKTDKSISLFTSLLKLASSNDSTNSAVSAKITNLLCGLVKQTVVFNHEEAKVNQIELLVHSLQLVTSIGGIDNPQIEKIWKLLDESVSRCVRAPFKYLDSSKDLQRISPLLVTIFEQWKFVDKETPYDLTSKWFAIFLRLLVIAGEPISAIEKLLEGVDLDCSISDYLPSGPYEENLQKLQNDPILKVEPNSSFFDSITTATLNKIQNNVKLPTSHLDLVGALFRVLQISNDNTLKLNQSGLEKIIVDILSKVGNYLIGNTEATKEFSNKKYWLPLFVTEKKNDKTAFISCTLAEIFSQLPEFNNFDFKAVINELLEKKTSSDEDSVIAACLWALDDSTLLKVLENDSLLLKQEALSLLSKRKVSLSSINIASLAQEPALLPLIEHFVSLNLVAFDNLEPLFTTLSSDHSRFSLFNLLSQNLDIIPQLLEFVILKDDPVLTCYVCSSLSAAAVSSIDNGTFMKLIEVAELYAIEHIKKEDFEIISFPQLLNIFVHSTSNIDAEVQQLILSYALTKYSNKYIQQIAEIIERFNCFQDDLVKVWINKSILFMTKVFSEVKQLPASFIAFLKSFKSLLSKSGSIQVANKSNLNTLLEAIFTRWVADETPLEFAAMIIVVASKASLETTKLLQIFIANDDIAFLNKNHATSRSRFLSALILWRLFEFDVSKNSSLSLQEKILMFYNGTNNAEDQLLYKILEKIETRLNSSWVDLVYTWDFLDSLTDEETELIGETKFIEKKKEGFIVTLSKTKITNTIENYCISNFEVPSLNSSGFGNWNKIQSFYEEVESAVSKKDSYNPLFLSLLIINNEELMTSSTDGEDQSPKVNMRKLVESELFAFILMNLSSQDKNLLQVTLNILSALLHSLQGDSSFKDKHIFELFLSKVVYTFKKTSELGERTNPQEFSPLIYSMISKLSIVLNNPGHFLYEKAFRWILKSPVIRKNEIPLFNEISTVLVTNDDASHYYKQLVWLIEGFVTGVKNKDDVNLLRNKNVFEWILNLESSPYITSQLKFLILDFIKSVQDVEDGADMLVTRYGGLANIEQQLSVDEHNGELITINNELVKNKQEVLNLKELGLRYSIIGQNKKRIANWTHDDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC23</accession>
    <name>A0A1E3PC23_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Actin-related protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_61013</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the actin family. ARP2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042176.1">
        <property type="nucleotide sequence ID" value="XM_019183095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC23"/>
    <dbReference type="STRING" id="683960.A0A1E3PC23"/>
    <dbReference type="GeneID" id="30200341"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051654">
        <property type="term" value="P:establishment of mitochondrion localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10220">
        <property type="entry name" value="ASKHA_NBD_Arp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000050">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000005">
        <property type="entry name" value="Actin-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="388" mass="43841" checksum="D2F83FCA8FB823F8" modified="2017-01-18" version="1">MSEQPIVLDQGTGFVKIGHGGSNFPDHTFPSIVGRPILRAEERTGDIEIKDIMCGDEASAVRSALQVSYPMENGIIRNWEDMEHLWDYAFYERLQEDTTGKKILLTEPPMNPLKNRERMCEVMFEKYNFGGVYVAIQAVLALYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLTRRLDVAGRDVTRHLIDLLSRRGYAFNRTADFETVRQIKEKLSYVSYDLELDAKLARDTTTLVESYELPDGRVIRVGSERFEAPECLFQPSLVDVEQPGVGELLFNTIQAADLDIRSSLYKAIVLSGGSSMYPGLPSRLEKELKQLWLTRVLHNDASRLDKFKVRIEDPPRRRHMVFIGGAVLANIMADKDHMWISKQEWEEQGPSILSKLGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCD4</accession>
    <name>A0A1E3PCD4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_26962</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042245.1">
        <property type="nucleotide sequence ID" value="XM_019181321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCD4"/>
    <dbReference type="STRING" id="683960.A0A1E3PCD4"/>
    <dbReference type="GeneID" id="30198567"/>
    <dbReference type="OrthoDB" id="429143at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF279">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="1">
        <location>
            <begin position="40"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ63038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="467" mass="52488" checksum="B9939E9FC3270D71" modified="2017-01-18" version="1">MTSNTYLPVPNPTKSFWLSLQESDSFSTYKSSESVPKKTDILIIGSGYSGASTAYNLLKEDPTLDITIFEARTVCSGATGRNGGHLKPYHHRMYYEYEKKHGIKVAAQVVNSEVEHLYTIKDLIEKEKIDCDFVLTRACDVYTDDQRLQNDYLSYRKLLENPFVNDEVKKTIQYLEGAECEVLSKVPNVKACFTAPAAHLWPWKFVTGLLKSCVTKGLKLYTNTTVTKVRKDELSGDFIVEAATGTTISKKVVFCTNAYTKAILKEFKDTIVPSRGVVTHIKPISTPIPQFPNTYLLFEKFDMNSDYLINRADGSVVVGGTDSLFIGLDGNYTDLYDTVDDSQIPYGAVEYFSGYMEQKFSTWNLVETVNDYTWCGILGFTKDSFPFVGELDFLNMKNAYISAGFSGHGMPRVFLSGKALARCILTGKSIQEVGGIPECYYASALRLQERGHSYKEEIEEYIESIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PD58</accession>
    <name>A0A1E3PD58_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_82831</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042099.1">
        <property type="nucleotide sequence ID" value="XM_019185447.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3PD58"/>
    <dbReference type="GeneID" id="30202693"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015440">
        <property type="term" value="F:ABC-type peptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000770">
        <property type="term" value="P:peptide pheromone export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000604">
        <property type="entry name" value="ABC transporter B family member 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001471">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF15">
        <property type="entry name" value="ALPHA-FACTOR-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="238"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="723"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="765"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="845"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="870"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="949"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="986"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="12"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="351"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="725"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="1048"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="673"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="684"/>
            <end position="700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1289" mass="144326" checksum="6A86439B129AF421" modified="2017-01-18" version="1">MLFSRSDIPWLIMGSIATIIGALAPAISSILMGRVFQVLAKFMKGEYHGFGDYMQAIRLSTMSLVIMGASCVPFVWTTISCWMQIGESQGLKARTNILETYITKPISWYEENDKVMGDLTQINRCVEELRAGTSEATALFLQSIVCMGALLGTSFYYSWSVTLVMLASSPLIVVFAYVFARLTEKFTKQENTETAFAAKILDWSLISSKLVRLFSTQSLEFNKFETSVLKCKRAFLKLILVTSANMGLMRFIMLCMFVQGFWFGSSQVRKGKVEAGHVLTCFTSCLMLGETLKSVLPQIVTIQKAKVAIRKIQQFVQIRKSKTMVSKFKTQIALDDTKNISLYPESCRGDIKFKSVEFAYPTRFDTKVLKNVTIHFPAGKTTFLVGRSGSGKSTLSSLLLNFYAPNKGRVEIDGFSVSNLNSRWLTDNITLVEQTCTLFKDTIRNNILMGKISSGVENVTEEQLNEACQMALLNEVIRDLDEGLDTVVGPNGIALSGGQQQRVALARARVRDTPILILDESVSALDIVLRELVIAAIKKWRVGKTTIILTHEYSQIGKDDYVYLMESGVVAEHGVRKELEQADGLFQRLTSLQDTTVEKLQDGKLEDLPESKPAFKSWEEEQRKTFLNQVGSRVSTQMFSTINPLSFLGQNLEHEVDTREILDRKIQRRRRLGSPEVTETLHTPIEKDLEKGEDKKDADERPDITPILTIIKKMYQSVDQKPILFVGLLFSILNGAVNPIFSYTFSKLLSGIVPQNDDVGSPRYLLKWSLVVIFLALFDGISTFVKEFLLHYSSEIWIFSLRKRAFRAISDQGLSWFGEKTNAPAEVSALLLNDARDLRSLVSQFLGIVSTASILASLGLIWALVSGWKLSLVCIAFLPAFVITSGLYAGFLQSSENEYKNAVAELENQTYETVKGIKTIRVLRLEKYFINKFHERAAELQRVSRKRSILTGMGVAVTSLLTFVVQGTLLYFGMRLVGKGEYTTDMLMQTFVLLIFSIMSCVQLINEIPDISRGQRAATYIFRLLDLEPSKTETIGSVIPEAEEKNIINFDHMNFCYPSLPDHKVLKNLSLNIKQGEHVAVIGESGSGKSTLTLLLTRLFDVPENSLFFEGVDINKIDIKWLRSAIALVDQKATFFDGTIRENITYGLEEVTDSELIDSLKLANIYDFVVSLPEGLDSRIDTSLMSGGQAQRLSIARALVRNPKLLILDECTSALDSEGASKIADLIKNNLKNRKDMTILMITHSEDMMKVSDKVVTMKAGTICEEGSFNELFNRRGELFRIVTAGMEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PDS3</accession>
    <name>A0A1E3PDS3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_82900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042224.1">
        <property type="nucleotide sequence ID" value="XM_019185459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PDS3"/>
    <dbReference type="GeneID" id="30202705"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ63017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="80" mass="9237" checksum="517AD7F3ADF4775C" modified="2017-01-18" version="1">MNVELLKFELFHQLNRDHTMDRLIRFRHGCKQYCPTMKTTVKAFFLSTNTIPWNTQFALVFPISSVLAKLGPERSSGCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU35</accession>
    <name>A0A1E3NU35_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_37061</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454216">
        <property type="protein sequence ID" value="ODQ56695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035902.1">
        <property type="nucleotide sequence ID" value="XM_019182509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU35"/>
    <dbReference type="STRING" id="683960.A0A1E3NU35"/>
    <dbReference type="GeneID" id="30199755"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF11">
        <property type="entry name" value="AMIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="5">
        <location>
            <begin position="73"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="3">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="228"/>
            <end position="231"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ56695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="549" mass="61677" checksum="0B9E6FADD61167FC" modified="2017-01-18" version="1">MEPWKQTARNKKNFVLSKIPEGWILQEIKTPDEEPNVCDYLDKLLDEREVYITNLTLKQLLSEQLSGKLSALEIAKAFTHRAALTHQITNCCTEVFFEQAYEQAEKLDNYLSKTGKLIGPFHGIPISLKDQLNIKGVATSIGYVHRLGNIIENDDDEAVLVKQLRGQGALFYMKTVVPMGMMLCDTFSGAYGPTQNALNRHYSPGGSSGGEGVVVKAKACILGIGTDIAGSLRTPAIYNGIWSIRPTINRFSYLNVQNSFANQDIIASTIGPMANSLEDLEYFGEHLLGVDYQWIHDPKVPPIPWKTSIELPSKLSFGFMKWNKITMPHPPIIRMMETVKKSLISQGHEVIEWEPPISHQELLDFSVKVFGVDGYQEIVKEAELSGEPIIPELLYLCGGEFPPPINSIESYWELAGQRYKYHQQYIKYWLSTKDKTSTGRPIDALLTPAWNCCAVPRGRMAKLNADYTVQFNVLDLPAIPMPLGKADSKLDKKDPNYKPVNKCDQEVYDSYDADFYDGANAAVQIVGLQKFTEERLIKLAKVVRDSVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU76</accession>
    <name>A0A1E3NU76_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL14m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_81564</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035936.1">
        <property type="nucleotide sequence ID" value="XM_019185256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU76"/>
    <dbReference type="STRING" id="683960.A0A1E3NU76"/>
    <dbReference type="GeneID" id="30202502"/>
    <dbReference type="OrthoDB" id="274765at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000005">
        <property type="entry name" value="50S ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005745">
        <property type="entry name" value="Ribosomal_uL14_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_uL14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01067">
        <property type="entry name" value="rplN_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L14/L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ56729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="132" mass="14338" checksum="E12D3DA9AD78DF40" modified="2017-01-18" version="1">MLYLKSMVKIIDNSGGLMAECIKVLRKSPMNYASVGDKVVVVVQKARPVNSQITGASASNRVKKGDIVHAVVVRTKQNVQRPDGSVMKFDDNACVLLNKNGEPLGTRISSVVSRQLKDKNYNKIVSLAPKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUI3</accession>
    <name>A0A1E3NUI3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2</fullName>
            <shortName evidence="8">Oligosaccharyl transferase subunit OST2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_86285</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Endoplasmic reticulum membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DAD/OST2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036068.1">
        <property type="nucleotide sequence ID" value="XM_019186032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUI3"/>
    <dbReference type="STRING" id="683960.A0A1E3NUI3"/>
    <dbReference type="GeneID" id="30203278"/>
    <dbReference type="OrthoDB" id="445566at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003038">
        <property type="entry name" value="DAD/Ost2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10705">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10705:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02109">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005588">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ56861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="119" mass="13549" checksum="BD7962B7A5C4D4B2" modified="2017-01-18" version="1">MAPKASKQTEFNNSIEKSIHSYFETLTPRLKLIDIFLAFLVIVALVQFAFVILVGSFPFNAFLGGFISTVGQFVLTVSLRLQSVPQNKEFFKRISPERALGDYIFASLILHFIIYHFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUM4</accession>
    <name>A0A1E3NUM4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_36961</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in cell wall beta(1-&gt;6) glucan synthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KRE9/KNH1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035937.1">
        <property type="nucleotide sequence ID" value="XM_019182496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUM4"/>
    <dbReference type="STRING" id="683960.A0A1E3NUM4"/>
    <dbReference type="GeneID" id="30199742"/>
    <dbReference type="OrthoDB" id="2432613at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042546">
        <property type="term" value="P:cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045328">
        <property type="entry name" value="Kre9/Knh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018466">
        <property type="entry name" value="Kre9/Knh1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008659">
        <property type="entry name" value="Kre9/Knh1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28154">
        <property type="entry name" value="CELL WALL SYNTHESIS PROTEIN KNH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28154:SF1">
        <property type="entry name" value="CELL WALL SYNTHESIS PROTEIN KNH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10342">
        <property type="entry name" value="Kre9_KNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05390">
        <property type="entry name" value="Kre9_KNH1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133527" evidence="5">
        <location>
            <begin position="20"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1-like N-terminal" evidence="7">
        <location>
            <begin position="26"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1 C-terminal" evidence="6">
        <location>
            <begin position="166"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="130"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="147"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="260"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ56730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="275" mass="29326" checksum="38CA922C6EDA91C5" modified="2017-01-18" version="1">MRFTKIFTLLFLLVASVVGDVSISKPTKGSSYTVSGGNAQVEISWEESNATPKLSDIEEYSFTICTGPNTDINGLQTIKKITASDVKGNSYTLSIPSNVGADGSYYVQVYATTKNGYTIHYSQRFTLQGMTGSKKPSGGDEVAPPAGQTSVNAGDQTSTLAPSDLSKSHQLFYTSQTGLTRYAPMQTQPGSTITASTWSRRYQTSSVSYFSTLKHSAQVLSTKTQDWSYTMSSAVNYASPAPDPSVAGWYNPSSKITPASLSSFSSGSDASSSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUU3</accession>
    <name>A0A1E3NUU3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_36883</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019182482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUU3"/>
    <dbReference type="STRING" id="683960.A0A1E3NUU3"/>
    <dbReference type="GeneID" id="30199728"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051880">
        <property type="term" value="F:G-quadruplex DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Euk_RNA-bind_prot"/>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
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        <location>
            <begin position="3"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="106"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="72"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="180"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="77"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ56908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="238" mass="26261" checksum="2D771D31032EA89E" modified="2017-01-18" version="1" fragment="single">EPATLFVGRLSWSVDDEWLKREFEPLGGVLSARVIFERASGKSRGYGYVDFESKSAAEKALKEYQGREIDGRPINLDMSTGKQHTSNNKAGDRASKFGDTPSAPSDTLFLGNLSFNANKDNIFNTFSEYGSVISVRIPTHPDTEQPKGFGYVQYSSIDEAKAAHQALNGEYIEGRPVRLDYSAPRDNSNRSFGGNDRKSFGGNDRKSFGGRERKSFTPTGSNRAPVTADFKGTKKTFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVG4</accession>
    <name>A0A1E3NVG4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_81339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019185219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVG4"/>
    <dbReference type="GeneID" id="30202465"/>
    <dbReference type="OrthoDB" id="3981276at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="127"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="209" mass="24764" checksum="93A544A70FB15AA3" modified="2017-01-18" version="1">MPDHDCISITEKTFDLSLDDTQIDDDDLSGDEQPLNSQILLDNTYDSLRNGKVQNKFKTVITNKADDDRITINRAVNGTIQYNEKKEQGNDHYLCKSLQVLQRISGTDIKINEEETNQISKLIYELSENSELKIQFYEKNYKFLENLYETLQKSNERVKIENKELRTQNESLKLRLDEMKNTRDQDEVVLRQEKEIRGYKRFIKELMER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVI0</accession>
    <name>A0A1E3NVI0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_71461</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454216">
        <property type="protein sequence ID" value="ODQ56702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035909.1">
        <property type="nucleotide sequence ID" value="XM_019184247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVI0"/>
    <dbReference type="STRING" id="683960.A0A1E3NVI0"/>
    <dbReference type="GeneID" id="30201493"/>
    <dbReference type="OrthoDB" id="410044at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070063">
        <property type="term" value="F:RNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.440">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002713">
        <property type="entry name" value="FF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036517">
        <property type="entry name" value="FF_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045148">
        <property type="entry name" value="TCRG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15377">
        <property type="entry name" value="TRANSCRIPTION ELONGATION REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15377:SF3">
        <property type="entry name" value="WW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01846">
        <property type="entry name" value="FF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00441">
        <property type="entry name" value="FF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81698">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="WW" evidence="3">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="132"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="156"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="173"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="216"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ56702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="472" mass="54562" checksum="8A8A3C005FF14503" modified="2017-01-18" version="1">MDAERGSGKLDNKQHDSRPKNQGANAKQKSHKGQKYVPDKRQKVEDIIRPTFKIDLAGTDWVLVFTNKGTHFFYNKTTKESHWRVEDAELNNSMKEIEKDNLLLLIAKSRGLKLDDDVEEKLQHELKAKEEFVEEPGQDDNDLIKNLVGGREDSTDQVVMKSTTPLVSGYSSSDDDSDEDEEKANEDEGEKGQQINVDPKDNSIDDINQIERITNDEETPLEDGESSEEENAFDLADLEGLSSDEEEKTSTQDSELLFLQLLQESSLNPYSPWELESLKIINDSRFHEIDSNKERKRIYDQWASAAVQQKEINALAQETAEEAEAEEEPLVEYIKFLETKELERLYVDFKKKWKKQLKKLKLGDKDKEKIYKEYIIYNKKSEDEKAGIFKSFLLGAKIFKKNVQEASDLSILQQFDDNKVKELDFNSAYKALHQLETQLNVSDSLKYNTKYYIVEIRKRISLLVEVAKLYTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVL3</accession>
    <name>A0A1E3NVL3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_36060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036408.1">
        <property type="nucleotide sequence ID" value="XM_019182392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVL3"/>
    <dbReference type="STRING" id="683960.A0A1E3NVL3"/>
    <dbReference type="GeneID" id="30199638"/>
    <dbReference type="OrthoDB" id="10256179at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053245">
        <property type="entry name" value="MitoProcess-Associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47563">
        <property type="entry name" value="PROTEIN FMP25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47563:SF1">
        <property type="entry name" value="PROTEIN FMP25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="380"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="601" mass="67751" checksum="DEB040D326D192A6" modified="2017-01-18" version="1">MLRTIRARPLIRNQVSRFHSSALRLNDSKKKPEQIDEAEMFADRLNTQPYYAKRDPSEYPEGEAPLSQEQIKKQNQKLEAMKGLIQGVLVIGAMFGGFELYQKWPNVKSWWANLNKEDEGFVPPKKNKPVVELPVITNKNDSSVPGLYIWGDNSNGITGDKNSISTKWPLRHPWFDGKLLKKVTIGEHSALAIDPKGDLIQWGNGYKRNSDPEYTIKGEGLQFAKISNGAIYALNKNNEILVIPESSKDQQDFKGLQRRNWAFISSTKNFTKVPLDLLQKGETIKDFETGAEHLVALTDKGRAFSAATGFNQLPKSQGQYGLPEYSHFNKPPAPNTLHEITLLNNSIEKNKKGKVDHIEPRFIKKIAVGNYHTLALDENGEVFSFGQNTFGQLGQPVNYDTEFITIPKKVELLSKHVKRDLFPDVIDIHAGGDTSFCTINPVKMFRLIRGKNNKVEEDEDALIDAFTVGFGNNLKGQIGNGHYVHAQFDPVKIKELSKFEDYNEETGKIEKINIDSWSTGFDHTFIKLGNKDVLFWGGNDNGQLGNGKKNRIPKPTPLPAIVEPSFTTETYKNLTFVNRLQLRENQQIVASANASAIYYTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVM3</accession>
    <name>A0A1E3NVM3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">25S rRNA adenine-N(1) methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">BMT2</name>
        <name evidence="5" type="ORF">WICANDRAFT_47655</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(1) position of an adenine present in helix 65 in 25S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the BMT2 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036370.1">
        <property type="nucleotide sequence ID" value="XM_019182604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVM3"/>
    <dbReference type="STRING" id="683960.A0A1E3NVM3"/>
    <dbReference type="GeneID" id="30199850"/>
    <dbReference type="OrthoDB" id="5954793at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016433">
        <property type="term" value="F:rRNA (adenine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03044">
        <property type="entry name" value="BMT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021867">
        <property type="entry name" value="Bmt2/SAMTOR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21008:SF1">
        <property type="entry name" value="25S RRNA (ADENINE(2142)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21008">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SENSOR UPSTREAM OF MTORC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11968">
        <property type="entry name" value="Bmt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="318" mass="36318" checksum="F439BA4A760D56BF" modified="2017-01-18" version="1">MGKRTGFLKKSKSITGKSFTKPSIKPSNTRKIIRRYHLLLNKKDIIYQKLSHIHQKDINDSNIGDFIKTRPKATTSGAINIPTEQELLSASNETSLPKLYLLLEKINYEIIEKGGLSKYQIASIYGQESKRGSDSSKWLFETIPELKEMKDTLKVLEIGSLSTQNVVSKYANSITRIDLNSNEPSILKQDFMERPLPKNNNEKFNLISCSLVLNFVPTAKGRGEMMQRFEKFLDKEADSPLVFIVLPLSCIKNSRYCDKAHFEKISNSLGYELIKYSEAKKLVYMVLKWTGKLEPKSFPKKKLHDGPAMNNFSIIIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVZ0</accession>
    <name>A0A1E3NVZ0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_19331</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019180825.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVZ0"/>
    <dbReference type="STRING" id="683960.A0A1E3NVZ0"/>
    <dbReference type="GeneID" id="30198071"/>
    <dbReference type="OrthoDB" id="2195431at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:maintenance of DNA trinucleotide repeats"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034398">
        <property type="term" value="P:telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF3">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY PROTEIN 18 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="115"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="636" mass="73453" checksum="E3277AFC1A72CF58" modified="2017-01-18" version="1" fragment="single">KKNSADALDALKKEKENYGFNINELYAKLEVQRLKDKSGSSKKESYIPKNDVLWAEKWRPKSFFELIGNDDTNRRMLKWVKEWSRVVFDKNYKPITTDDSSATNSKFIDPFGRPNKKIMLIHGPPGLGKTTVAHVIAKQAGYEIMEVNASDERSGQRVRDKIVNSLASQTFSGKPVCLIADEVDGAAEFGFIKVLVDLINEDQKAIYKFQHSDGDRSSKEKSKKKPKFLLRPIIAICNDLYAPSLEKLRMHSEIIAFKAPSERDLRERLRDICKHEKISLTNQQLQEIVLLTNRDIRSCLNILQFGGGLNSSNDLRKKDSQVSWFAIVNEIFRRRRDLKKGVQFKELANIISINNNYDKIINGCFQSYHDITYNDSGMSKPAMISDWLYFGDIMGKTTYESIGDLSYYSSQVALQFFNQFSDLGNRETIMIKSDYEHFESRRANFNLLRLLHSNVNVHLRTFLKLQQLPTEVLPLLDYIITPELKSLNNIKDYDKRKLHDAIDSLQGFGLRITEAKDEAFNEIYTTYPQFSSISKFDTLSTKKLTQKRTQLFPVLQKEMDLVRVKKRAFSEVEKDAEAKSSKTGADFALLKNQYEKIAEAELKAKKLKTEVKIWVKYHEGFSNAVRKPVKWKSLWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW69</accession>
    <name>A0A1E3NW69_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor BYE1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_64732</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Negative regulator of transcription elongation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BYE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW69"/>
    <dbReference type="STRING" id="683960.A0A1E3NW69"/>
    <dbReference type="GeneID" id="30201039"/>
    <dbReference type="OrthoDB" id="79252at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001139">
        <property type="term" value="F:RNA polymerase II complex recruiting activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031440">
        <property type="term" value="P:regulation of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.30">
        <property type="entry name" value="Transcription elongation factor S-II, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012921">
        <property type="entry name" value="SPOC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036575">
        <property type="entry name" value="TFIIS_cen_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477:SF11">
        <property type="entry name" value="TRANSCRIPTION FACTOR BYE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477">
        <property type="entry name" value="TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07744">
        <property type="entry name" value="SPOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07500">
        <property type="entry name" value="TFIIS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00510">
        <property type="entry name" value="TFS2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46942">
        <property type="entry name" value="Elongation factor TFIIS domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="9">
        <location>
            <begin position="54"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS central" evidence="10">
        <location>
            <begin position="246"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="116"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="424"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="129"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="171"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ57378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="724" mass="83859" checksum="461082CA9338AFC2" modified="2017-01-18" version="1">MAEIRRSSRANKGTNTRLSEQIKYEELIAKKSKKARRNSSGDEEVGDEEDEDGAVQCLVCGTTDDNYDEEQDDRDMIQCDNCQTWQHTECILGKQSKVIPDEYKCNVCDPANESYKNLSFKIDPSTIKPKPKTIKKIKKKSRRNSSNGKDEDEEEDDEFKDNDKVDDDDKDFSADDAEFKVKSERKQRKRSRSKSTDHNNEDDDDDDYERKKKSKSPKHRGRQSISEINAAESKAREIDAKTKDAVKRRFQTMFLKLLPGSNVNLKDENDDENTIESLSLKWATKLEDELYKVYHDPETLKLTPAYKEASARIYSNVIDVKNSKLRLSIINKVLPFDKLVRMPVQELLNPDLRKEKEEAIKESMTLATLEAPTVSNIRRTHKGDILLEKDDSNAQTQYDFNVGMNIDNDEKKFTKSKNTVINERKESSIYNNNGNNNDEDDEFDNSFDKDDNGDGTQETYSRDRSHSLQDDDAILQILGETEKEVKQQQDESNSFNYETPIWSGQTVFVDVTNFYSVVKLSKTNLKFKDYNKISEILIDHDLPLEIQGRLDLRKADDYIRKISDSRKLILLELENNESNDKNFNKLFHYFYSRLKVGVVKFRSSFIKDSYLIATKDDHVPRFFENVLNKTDKSSTPRLFIVFVVKQELIEKIDVNRDSKSASSLNTPPNTSTTTATVPSFEFLSRLNPHELDLVNKIFDERPETRVDVNLLISYLQQKLKRQTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWA1</accession>
    <name>A0A1E3NWA1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_96705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036611.1">
        <property type="nucleotide sequence ID" value="XM_019186527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWA1"/>
    <dbReference type="STRING" id="683960.A0A1E3NWA1"/>
    <dbReference type="GeneID" id="30203773"/>
    <dbReference type="OrthoDB" id="3972203at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="139" mass="16170" checksum="C12AA76F4C5687BA" modified="2017-01-18" version="1" fragment="single">MNTTNSTNPAIGRPSSSNLSEEDLKFDQHIGGFDTNFNIFQHFNHNQRPIPKFLIYQSPALKAAPIKQDEWDKSNQQEMFRLEQNTSDVSTLYEDFQKMRENERKVMEQKGLVDKEDTRKTLEDAISFQGSCLEMCPVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWF6</accession>
    <name>A0A1E3NWF6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_81245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036219.1">
        <property type="nucleotide sequence ID" value="XM_019185201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWF6"/>
    <dbReference type="GeneID" id="30202447"/>
    <dbReference type="OrthoDB" id="3982491at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="229" mass="26675" checksum="CFBCB3758C3FDEDA" modified="2017-01-18" version="1">MSFNEEKQDGVDIEDLTSRKNSETEVLKALELREAELDEYKLKLITRTNQASNQILENHHSMTVENRILNFFIFAFTIFIVLQFSLILIMKLDIDWPSLLQFISFEDFYQISWLCILVIVRCYIGVKIFRRLVKTNILFKLLNYQFMGFLKSTLMTMGINILVSMDYIGNHEHVNRVLDIVKQMSTVYKKINCLFVKSSKVTIFAVFNNSAGTIGDEDSPDVHDLQSFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWG4</accession>
    <name>A0A1E3NWG4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein L10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_12264</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036683.1">
        <property type="nucleotide sequence ID" value="XM_019180528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWG4"/>
    <dbReference type="STRING" id="683960.A0A1E3NWG4"/>
    <dbReference type="GeneID" id="30197774"/>
    <dbReference type="OrthoDB" id="360689at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05797">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047865">
        <property type="entry name" value="Ribosomal_uL10_bac_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11560">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="21987" checksum="83569FBAF015D6C5" modified="2017-01-18" version="1" fragment="single">SRKTYLINRYKTLLQDTPIVLFAHHNNLVKTDENTFRNQIREVGGEFTVIRNNLLLAYLKAENEKYPSSAEAYKKTRFLDHPLKPFLVGPTAMITVQENNPQVVSKILKTLKTANEKLFVVGARIENRVFDLPQVNNFKDLPSKEQLHSELAGMLTVLSGAGLVQTLQSASQHLYLTLESHRKNNDPSEKKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWN3</accession>
    <name>A0A1E3NWN3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_85688</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the YIM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036751.1">
        <property type="nucleotide sequence ID" value="XM_019185932.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWN3"/>
    <dbReference type="STRING" id="683960.A0A1E3NWN3"/>
    <dbReference type="GeneID" id="30203178"/>
    <dbReference type="OrthoDB" id="3509362at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050700">
        <property type="entry name" value="YIM1/Zinc_Alcohol_DH_Fams"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695:SF294">
        <property type="entry name" value="RETICULON-4-INTERACTING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="4">
        <location>
            <begin position="15"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="352" mass="39112" checksum="486EF317AA0AF4BD" modified="2017-01-18" version="1">MSKSLTFKRLAYNNGCSMKIQEKTFNLPTDLASDEIIVKISNAALNPVDLLLYHTSRYIFFKRGVKGVGRDFSGTVYATGSNISEYKEGDLVSGLYMPVYGEQGTIAQYLKLKPKENPMGKIPSTLSLQQASAFPLVFATAVSILRKFHVPDENSRVLVIGGATSVGQYVLQLLKNHHHAKSIISVNSESSSSLTKSFGADTVIDYNKQDVAQTALSLVQNDFGGEKFDLIIDCVGNNDIFPVINYVLKPKTEKAGYVTIVGDQIADYNSSIFSFLKVANLGFFSKMVPYFRSYNYGMVATAEDFYPLAKKLFEENKLKVVIDSTFTWSDYEKAYEKLSAHKARGKIVLNFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWW0</accession>
    <name>A0A1E3NWW0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_64965</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036823.1">
        <property type="nucleotide sequence ID" value="XM_019183841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWW0"/>
    <dbReference type="STRING" id="683960.A0A1E3NWW0"/>
    <dbReference type="GeneID" id="30201087"/>
    <dbReference type="OrthoDB" id="3365616at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22952">
        <property type="entry name" value="ART10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="36274" checksum="54F44699C65B9FFB" modified="2017-01-18" version="1">MLPGKYEYDFEIPLPQVNYCSKSKDNAFVRTPKHDEYPLPPTVDDINQLANVAYEVTAILNSSKFFSMNAKTSLTVRLGTPMSDLLVRKSKSISYWRDSVSFEKKLPSGEVLNIPVFLEIRSNGFCETVGEMPTFKLFVLSEIQPENYRSMELEDAGCVYLRSIEVLIKKHTKVRAYEATEEEIKEYNICSVEGLSHRLDLSHAHRSNAVEKNDGSSLYELEIPSSIYQNVAIPKSFTPTFEFCNIKRDYEITIKVGLSGTAKAWEPEVFELQRELTLLSGVCDQIDKSEKELLMEQQRNPQLMEELPPPSYEKIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX70</accession>
    <name>A0A1E3NX70_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_81061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036940.1">
        <property type="nucleotide sequence ID" value="XM_019185167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX70"/>
    <dbReference type="STRING" id="683960.A0A1E3NX70"/>
    <dbReference type="GeneID" id="30202413"/>
    <dbReference type="OrthoDB" id="9974981at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051609">
        <property type="entry name" value="NmrA/Isoflavone_reductase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47706:SF9">
        <property type="entry name" value="NMRA-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47706">
        <property type="entry name" value="NMRA-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="297" mass="33243" checksum="39781DC07CB0671D" modified="2017-01-18" version="1">MTTIAVFGLNGFLGKPIIEALLREPFISKIKTPIRLLTSSDKKPIESSKVQYYNVKEVGYDKPLDGVDVVVNLGSVPGDPKDDAFLKSIISHKVKLYIPSQFGTDLDATSETLPNFLQPKTTHSKAARDAGIKTVDIITGLFINEDGFLPFAPLYVLGHDVEKTKEATIRGDKNTLINPSYFKDIGNTVAALATKDNFASIPDKVRIYSDRILATELIKHYEKVNNVQLTTKYVSTADTVKEAKEKYSKGFKFDDFVFYLITLLAEGEGKGLIFEKENEKEFVNPKESLFKWTKFQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXS7</accession>
    <name>A0A1E3NXS7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_96635</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the neutral sphingomyelinase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036574.1">
        <property type="nucleotide sequence ID" value="XM_019186523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXS7"/>
    <dbReference type="STRING" id="683960.A0A1E3NXS7"/>
    <dbReference type="GeneID" id="30203769"/>
    <dbReference type="OrthoDB" id="387657at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052714">
        <property type="term" value="F:mannosyl-inositol phosphorylceramide phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004767">
        <property type="term" value="F:sphingomyelin phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072711">
        <property type="term" value="P:cellular response to hydroxyurea"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090266">
        <property type="term" value="P:regulation of mitotic cell cycle spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030149">
        <property type="term" value="P:sphingolipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000073">
        <property type="entry name" value="Inositol phosphosphingolipid phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038772">
        <property type="entry name" value="Sph/SMPD2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320:SF24">
        <property type="entry name" value="PHOSPHODIESTERASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320">
        <property type="entry name" value="SPHINGOMYELINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="15">
        <location>
            <begin position="33"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ57367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="442" mass="49981" checksum="7270F55E51A4E743" modified="2017-01-18" version="1">MDSARSSKGSKMEWSIDVESTPAPTGPPQVKFLTLNTWGLKFVSKHRKERLKAIGDKLAHEQLNYDIVALQEVWCKEDWDYIDQVCQEFFPYRRIFYSGIIAGPGLAILSKIPIDSSFLYRFPINGRPSAFWRGDWYVGKSVAVTLLRPLNDESFPLAILNSHMHAPYSLEGDAAYSCHRSCQAWDFAKISNMLSKAGYAVVIVGDLNSRAGSLPHKFLTLETTLVDSWEQLNGTQDLNKIKHLSPKLQVEVGGCTCDSILNTWRAHRDPTEACRLDYALVDSSKLKTISASVQFTEQIPNIGSYSDHFAYSCTLQFLSRNSYKEVIRSNSLTVNSVEYYNEMLELLDDYKKTALWQKKWRGWHFVLSILAIIAIHVVVTFTANIAGWSSIFWVFGAVVIGITGVVDGLISYLFGRSELRALEEVKQEVLDSKRSLQTVVYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXU2</accession>
    <name>A0A1E3NXU2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_101755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036594.1">
        <property type="nucleotide sequence ID" value="XM_019180333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXU2"/>
    <dbReference type="STRING" id="683960.A0A1E3NXU2"/>
    <dbReference type="GeneID" id="30197579"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="456" mass="50407" checksum="1D2C54C954F2F930" modified="2017-01-18" version="1">MSDETSTVDTSFHSTFSSTSQDNKSIDHELHSKKSSLLLKDTSSNHLTTPRVSRTLDNQTSPGSLPRYPGTLHVNALTSADNSLKDPNEEGLSQQDNDNNDDEANDSNNSKTLDPGKPSNNTNQRVNELINSLDEPIFFKRVSTMSTDSTNSTNSAQSYESLPNNSNTNDDITVGVVNTATATTFQKGTPTTRQVNNKSPELKSTDDEIGEEGSNTLGQDDQIPDLKKELANLEVPSEAKETGLQQPVQIEPLQRSSKVESTETTTKKEETDPIETSSLRSEEMETEKPIIQSSSHNVSSQELLTHDLKIQNPTLTTSSFVSTDPGSNFENKIIDDFIGSSTERLPPSSKYSENFDNEKFEQFEKPIAASSNINISSLGSSTTNLSEEIGDLTIQEEDVQQPQTGNNTSIMLQKLQIILQILHQYPLQISLHLKSHKKDQLQTLSLQRFPYCHNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY11</accession>
    <name>A0A1E3NY11_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small COPII coat GTPase SAR1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Small COPII coat GTPase sar1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">WICANDRAFT_34391</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPII-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 21">Belongs to the small GTPase superfamily. SAR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037263.1">
        <property type="nucleotide sequence ID" value="XM_019182180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY11"/>
    <dbReference type="STRING" id="683960.A0A1E3NY11"/>
    <dbReference type="GeneID" id="30199426"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <location>
            <begin position="27"/>
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            <name>GTP</name>
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    <sequence length="189" mass="21473" checksum="66A67105A458DAA1" modified="2017-01-18" version="1">MWIFDWFQDVLASLGLWNKHAKLLFLGLDNAGKTTLLHMLKNDRLATLQPTLHPTSEELAIGTVRFTTFDLGGHQQARRVWKDYFPDVNGIVFLVDCADPARFEESKAELDSLLGIEELQKVPFLILGNKIDSPRAVSENEVRQALGLYGTTGKGKITIENQRPIELFMCSVVMRQGYGEAFRWLSQYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYL1</accession>
    <name>A0A1E3NYL1_WICAA</name>
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        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL23</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">60S ribosomal protein L25</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_64430</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ58291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_019183734.1"/>
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        <property type="term" value="C:cytosolic large ribosomal subunit"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:structural constituent of ribosome"/>
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    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:translation"/>
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        <property type="entry name" value="60S ribosomal protein L23a"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_uL23_A"/>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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        <property type="entry name" value="Ribosomal_uL23-like"/>
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        <property type="entry name" value="Ribosomal_uL23_CS"/>
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        <property type="entry name" value="Ribosomal_uL23_N"/>
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        <property type="entry name" value="PRK14548.1"/>
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        <property type="entry name" value="60S RIBOSOMAL PROTEIN L23A"/>
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        <property type="entry name" value="Ribosomal_L23"/>
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        <property type="entry name" value="Ribosomal_L23eN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00050">
        <property type="entry name" value="RIBOSOMAL_L23"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL23 N-terminal" evidence="10">
        <location>
            <begin position="4"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00075004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="142" mass="15833" checksum="BDA80D46478F2AE0" modified="2017-01-18" version="1">MAPSTKAQAAKKAVVKGTNGKKTLKIRTNTTFRLPKTLKLARSPRYASKSVPHYERLDSYKVIVAPIASETAMKKVEEANILVFQVDLKSNKHQIKKAVKELYEVDVLSVNTLVRPNGTKKAYVRLTADYDALDIANKIGYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYP7</accession>
    <name>A0A1E3NYP7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF155 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_22922</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037327.1">
        <property type="nucleotide sequence ID" value="XM_019180967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYP7"/>
    <dbReference type="GeneID" id="30198213"/>
    <dbReference type="OrthoDB" id="18302at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF15">
        <property type="entry name" value="SPORULATION PROTEIN RMD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="DUF155" evidence="3">
        <location>
            <begin position="135"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="357" mass="41513" checksum="0B49ABAEF987082B" modified="2017-01-18" version="1" fragment="single">LGPQRSSRTTQKLKLLPEEPITDTVDGSRDVYSQVNRITDTPARRDAERLGKNQRSLLPRVTAYCTASSYKMKDLIRWLKDNKRTFESHPKLFDECLYTPFVYKDWRGDKRMDKDLIRLDDEGGEITIDDRHTDVFIFEYGVVVLWGFTLREEQAFLNDLEKFENEKLAQEDIQIEEFNYYITKSYQPRIYNDFITLRDGSNYMLKLSISHAISQSVKISLFEELVDNTIEDTQDIPQQIALAGKVEMNKDEIMKSIGELFILRININLHGSVLDSPEIMWAEPHLEPIYQATRGYLEINQRVALLNQRLEVISDLLQMLKEQLGHSNEEYLEFIVIILVGVEVLVSVINIVVDIIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYQ8</accession>
    <name>A0A1E3NYQ8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycoside hydrolase family 76 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_64288</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037347.1">
        <property type="nucleotide sequence ID" value="XM_019183701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYQ8"/>
    <dbReference type="STRING" id="683960.A0A1E3NYQ8"/>
    <dbReference type="GeneID" id="30200947"/>
    <dbReference type="OrthoDB" id="9984024at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005198">
        <property type="entry name" value="Glyco_hydro_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053169">
        <property type="entry name" value="MUG_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47791">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 191 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47791:SF3">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 191 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03663">
        <property type="entry name" value="Glyco_hydro_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="417" mass="47313" checksum="16F5C4D5F7DBE811" modified="2017-01-18" version="1">MSGPHPPVSNPNLEGYNVVATFWKRFWNSNKDTFTTPRPKCSGVYGDEGKFNVWSVAVATQAVVDGARIYPQELGPLIDPCIKALYKYRSPQFYGYCAVENFQGNNDIYYDDDAQVAFAIIAAYEVTGNKEYLDSGRELVRYLMGGLNNDSKAKVKGGILWHRDKPYISAISTSEVALAALRIAKFIPNEEKIYVDFAAKTTDWLLDVLLDKGDYLILDGIDKNSDGANGMKWTYNTGTALSAASLLYEYTKDKKWLKHANELAAAATNRNKSLFCRDYNEMDRRYWRDPSYFIQLLFEGFADYLLVVGNEAPESTANAIKRELKRHLEFFRKYHYDPNDGLYFQMFEAHRISKEIWELYCEQFGSTKGFDPNNEERAQEGDGGPGDKPLVKTLIGSGAAARIWFQAARVIPDIPYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYX4</accession>
    <name>A0A1E3NYX4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Protein transport protein SEC24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_35193</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SEC23/SEC24 family. SEC24 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036994.1">
        <property type="nucleotide sequence ID" value="XM_019182280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYX4"/>
    <dbReference type="STRING" id="683960.A0A1E3NYX4"/>
    <dbReference type="GeneID" id="30199526"/>
    <dbReference type="OrthoDB" id="49016at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990753">
        <property type="term" value="C:equatorial cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01479">
        <property type="entry name" value="Sec24-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000049">
        <property type="entry name" value="Vesicle coat component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041742">
        <property type="entry name" value="Sec24-like_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803:SF39">
        <property type="entry name" value="SECRETORY 24AB, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="13">
        <location>
            <begin position="157"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="14">
        <location>
            <begin position="231"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="16">
        <location>
            <begin position="479"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="15">
        <location>
            <begin position="572"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="12">
        <location>
            <begin position="702"/>
            <end position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ57787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="845" mass="92899" checksum="063A4647794C2DC8" modified="2017-01-18" version="1">MNIAGAPQYGQPAYGQQYPQVGAAPIGTPNAGGAYPGAAGGYNYNGVTSPHDATAATRQALPLNQLYQTDLLKELPPPIHDLTLPPPPLVIPADTSLIPNNPNANTPPDFIRSTLNAIPTTHSLLKKSKLPLAVVLRPYISLQDETNPVKLVSDTVIARCRRCRTYINPFVTFTEQNRRWRCNLCSLQNDVPKAFDVDDHTGLITNRYERNELNHSVVEFIAPPEYMVRAPQPLSYVFVIDVSIASVKSGLLATVATTILETLDRIPNSDSRSRIAIIGVDSTLHYFNIPEDSEQGECSLFVVPDLDEPFVPSPDSLLVNIKAARQNIEKLLSNLHNLFADTINPSFALGPALKSAHNLTQSSGGKVVAFASTLPNVGVGKLVQRDEESVSDKPKEASALLSANDSFYKSFAVDCNKSQVSVEFFLTSGTYQDVATLSNLPRFTAGQTHYYPSWTANNIEDVTKLSKEISNTLSSDIALDAVLRTRGSSGLRMSAFYGNFFNRSSDLCSFPTFPRDQGYVIEISIEENITKPVVYLQTAVLHTTNFGERRIRVITTAIPTTSNISEVYASADQLALTNYYTQKAVEKVYSSSLNDARDLLSKYLLDLLNVYKKEVVAGNLGGASPLMFSTNLRMLPLLLHSLSKHIGLRPGKVPSDHRANALNLLASLPLPQLVRYIYPTVYSLHDIPDEAGLPDSETNQIVLPNAINSGAEHFERYGLYLINNTTELFLWIGGDAVPDLVNDVFGVPDIFQVPIGKYELPVLDNEFNEKVRNIIAKVREGDDTVLYQNLYIVRGGSANEPINAANAREVSSLRLWAASQLVEDRINNQPSYREFLSATKDKLAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZN7</accession>
    <name>A0A1E3NZN7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">prephenate dehydratase</fullName>
            <ecNumber evidence="2">4.2.1.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_34599</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-phenylalanine biosynthesis; phenylpyruvate from prephenate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.51" evidence="2"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037691.1">
        <property type="nucleotide sequence ID" value="XM_019182206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZN7"/>
    <dbReference type="STRING" id="683960.A0A1E3NZN7"/>
    <dbReference type="GeneID" id="30199452"/>
    <dbReference type="OrthoDB" id="983542at2759"/>
    <dbReference type="UniPathway" id="UPA00121">
        <property type="reaction ID" value="UER00345"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04905">
        <property type="entry name" value="ACT_CM-PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13532">
        <property type="entry name" value="PBP2_PDT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000254">
        <property type="entry name" value="Prephenate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008242">
        <property type="entry name" value="Chor_mutase/pphenate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001086">
        <property type="entry name" value="Preph_deHydtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008865">
        <property type="entry name" value="PRK11898.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022">
        <property type="entry name" value="PREPHENATE DEHYDRATASE P PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022:SF19">
        <property type="entry name" value="PREPHENATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00800">
        <property type="entry name" value="PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001500">
        <property type="entry name" value="Chor_mut_pdt_Ppr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51171">
        <property type="entry name" value="PREPHENATE_DEHYDR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate dehydratase" evidence="7">
        <location>
            <begin position="7"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="8">
        <location>
            <begin position="207"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="296" mass="33540" checksum="99BB027245616AF1" modified="2017-01-18" version="1">MSSSDIKVAYLGPEGTYTHQAALQQFGEKTTLLPQKTINDCFQKVNDDEVDYSVVPFENSTNGQVMYTYDLLRDWLIPAKKDSNSTFKVVAEQFVSIHHCVLTHAKDISQVDKILTHPQAWGQVTDWLISEGKHLERIDSSSTSRAAEIASNEGVQSAAIASEYASKVHNIPILYKNIENNKENTTRFLILSKKDTKIIDTKYITLLAFTVEHDDAGALVKALDILSKHDINMTSITSRPSLNKLWQYVFFIEFWGDSYEDENVKAAVKEFNERSLSSVVIGTFPRNKRYYGEQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZN8</accession>
    <name>A0A1E3NZN8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_63086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037782.1">
        <property type="nucleotide sequence ID" value="XM_019183487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZN8"/>
    <dbReference type="GeneID" id="30200733"/>
    <dbReference type="OrthoDB" id="4063132at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018304">
        <property type="entry name" value="Rtt102"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09510">
        <property type="entry name" value="Rtt102p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="251" mass="28038" checksum="E0797C44E25A40D8" modified="2017-01-18" version="1">MSVRQSLYGQSLVRNANALNFNNDSSQEKHWKRDWYSPSKNIPQSTSITPSATPTPFGDERDTPSLEQQTFNFKVKAWTYDNDYKPIVDGEEEDLLDLEEFSIERKRDANGLTDDAIRGAVGSGESIPGISNGNNTKTEDKSEKDPKVEAPAVESNQPEQQQKTIEENSEAINPIQEQEKRESGASKSEGETAVATDIQTPAQPTLQEPQEQQEQQEIQEPEVNTTVPEAKEDEKINDEPKKDSEGDVVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZP0</accession>
    <name>A0A1E3NZP0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">5-demethoxyubiquinone hydroxylase, mitochondrial</fullName>
            <shortName evidence="8">DMQ hydroxylase</shortName>
            <ecNumber evidence="8">1.14.99.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Ubiquinone biosynthesis monooxygenase COQ7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">COQ7</name>
        <name evidence="9" type="ORF">WICANDRAFT_32664</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the hydroxylation of 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) during ubiquinone biosynthesis. Has also a structural role in the COQ enzyme complex, stabilizing other COQ polypeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 5-methoxy-2-methyl-3-(all-trans-polyprenyl)benzene-1,4-diol + AH2 + O2 = a 3-demethylubiquinol + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:50908"/>
            <dbReference type="Rhea" id="RHEA-COMP:10859"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:84167"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="1.14.99.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="8">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion inner membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the COQ7 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.60" evidence="8"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZP0"/>
    <dbReference type="STRING" id="683960.A0A1E3NZP0"/>
    <dbReference type="OrthoDB" id="275371at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008682">
        <property type="term" value="F:3-demethoxyubiquinol 3-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160224">
        <property type="term" value="F:3-demethoxyubiquinone 3-hydroxylase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01042">
        <property type="entry name" value="DMQH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01658">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011566">
        <property type="entry name" value="Ubq_synth_Coq7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11237:SF4">
        <property type="entry name" value="5-DEMETHOXYUBIQUINONE HYDROXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11237">
        <property type="entry name" value="COENZYME Q10 BIOSYNTHESIS PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03232">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="238" mass="26911" checksum="14CDCA06F56E8642" modified="2017-01-18" version="1">MPSIKTLPKNCKFSTLSQLRAQINTQPVPPPSSPKQYEYSSLSPHQKAFLDRVIRVDQAGELGANYIYAGQYSILQHTRPELKTILKHMWEQEIHHHNTFNKLQVENRVRPSLFTPLWKLGAYSIGVGTALIGKEAAMACTVAVETVIGGHYNDQLRVLMNKFSDIQAKDLNTGEIINGLESEELQNLKNTIKNFRDDELEHLDTAVENDAEAAVPYWLLTEGIKLTCRLAIWTAERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZU1</accession>
    <name>A0A1E3NZU1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_34741</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037717.1">
        <property type="nucleotide sequence ID" value="XM_019182223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZU1"/>
    <dbReference type="STRING" id="683960.A0A1E3NZU1"/>
    <dbReference type="GeneID" id="30199469"/>
    <dbReference type="OrthoDB" id="3364892at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF26">
        <property type="entry name" value="CARRIER PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G07710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="176"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ58510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="48916" checksum="6A43860C5818D42C" modified="2017-01-18" version="1">MVVTGAHLNSPSSSNGQSNTPRLAGPSFLGTSYIINSSLEEEDAQHIQPELSNKGTRVVGFGSAGIRALMYQLTSLYLRTPPAKLFRPTRIDYMAITRLFVHGSMTQTTKRPYNFYKDSSIGVFVSAIKKFGWRFIPDTILPPLIANSATGVVLYTTYLVSLQHFNGEKKNFLEDPSPIDTLRAGFVAGTFQSMAAAPIDAIYTRSSANELLKGQHENLWKFGLKKLHEIGVSGVFAGYALSFMKESIGFAIYFCSFETIKNQGYHFTRDVFINFKYYKEKVMSPYQFKLSKEEYTKKVEQEKKFKFLKTSFVFLAGMTASLSLQIIQYPITKIQNIHLSRLEAFDIYGTSLPNRGKPITRSRMLTIYYNTYLDTLEHVLFIQSNSKLSWYDWLYRGFRRHALSTIPATTIGLVVLEALRNKLAVEIEPLAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P008</accession>
    <name>A0A1E3P008_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_63294</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL33 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037993.1">
        <property type="nucleotide sequence ID" value="XM_019183523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P008"/>
    <dbReference type="STRING" id="683960.A0A1E3P008"/>
    <dbReference type="GeneID" id="30200769"/>
    <dbReference type="OrthoDB" id="5368485at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.120">
        <property type="entry name" value="Ribosomal protein L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038584">
        <property type="entry name" value="Ribosomal_bL33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016445">
        <property type="entry name" value="Rog1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF20">
        <property type="entry name" value="LIPASE YDR444W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005412">
        <property type="entry name" value="UCP005412_abhydr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="DUF676" evidence="7">
        <location>
            <begin position="223"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="467"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="614"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="467"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="486"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="540"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="568"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="615"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="862" mass="98414" checksum="78BAEA83894C5BD9" modified="2017-01-18" version="1">MEPSKWKIGSTFSSISSSSSPKSPSGSYFQNQQHQQIQSRLINENVHYRNQGKIGIGEINRFTVQYEASIDNGNVLEDDLGEYLWLRVRNIEMQVFRPIYFTGPFSFYIDVTPYNFDHKKDFDEPIEFNNDIKPGQSFKAKLRLNKNSLVRDRVYFWTIDVVAQLAMTTKFTINYDFMIGYDYKLLRKVTSDTVPDEAKLKMINVVRQDTKELWQKPPRRPDDHVHLVVVTHGIFSNVGADMLYIKEQIEKMCDLNEASNVIVRGYDGNVGKSEKGIRYLGKRVAKFVLDLCDASKYKIDRISFVGHSLGGPVQAYAISQIVSMRPEFFNAIQPINFISLAVPFLGILSDFPVAVSWALDVGALGRTGRDLTLSHRFPSFVKKFKRDADPDEPDTNTKITAKPILEVIPLGPAHYVFESFQNRTVYANAINDGIVPLRTAALLYLDWRGLGDLQKLKKKYEELTLKDDNGQHGERARKGSVGEIPEDSKEDKRHIVANDQKQNKDQSHNVSETELKRQEGENNGTVTFAESKVARAENPENVSDTNLIKSSRSGELASSAKTTVDTGSSGVNNSSSSKTNSSSIFKYPTLIFKEQPKKSKRKKLKKYIRTQTKTSTKDFEDQEDQNHNSDWVDVNDGDEETQDEMSEFNIPPAASTFLSAANVMMSPIPKEDYLMHPGQRIPTIFHDKRYTFEDLPPPHFAKKLKNAKAFKSFIKRDKNVIEERIAREWHWKMEWRKVLVTLRPDAHNNIAVRRKFSNAFGWGVIDHLVENHFGEEAIEREIKFMDNVRTSAKAKVKQTVVKLVSTAFTGFCRQVSVARGAPIVKQVRYDPIAKRHVVFQEAKKRKIAERKPISFSKAPKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P049</accession>
    <name>A0A1E3P049_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="8">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_84414</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the peptidase C12 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="8"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037851.1">
        <property type="nucleotide sequence ID" value="XM_019185706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P049"/>
    <dbReference type="STRING" id="683960.A0A1E3P049"/>
    <dbReference type="GeneID" id="30202952"/>
    <dbReference type="OrthoDB" id="427186at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09616">
        <property type="entry name" value="Peptidase_C12_UCH_L1_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.532.10:FF:000006">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF17">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00707">
        <property type="entry name" value="UBCTHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7 8" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UCH catalytic" evidence="10">
        <location>
            <begin position="4"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="119"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="7">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="231" mass="26131" checksum="5ED169DE256B06BE" modified="2017-01-18" version="1">MSQSVIPLESNPEIFTQFAHKLGLSQSFVFHDVYSLTDKDLLSFIPRPSPAIILLFPITPDYEKARLAEEEKSKGSTDDENVVWFKQTVKNACGLYALLNSISNLDEVSYEPNSRIVEFKKSYKDISKLEQLVENLKKDYTDAATSGETQAPDAEEDTSLHFIAFIKKDGHIYEMDGRRKGPLDLGEAQESNSEDVLGESTVVERFQRYIELSDDENKLNFSLMALAPSFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P066</accession>
    <name>A0A1E3P066_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_33803</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Protein modification.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037464.1">
        <property type="nucleotide sequence ID" value="XM_019182114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P066"/>
    <dbReference type="STRING" id="683960.A0A1E3P066"/>
    <dbReference type="GeneID" id="30199360"/>
    <dbReference type="OrthoDB" id="406833at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="6">
        <location>
            <begin position="2"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="120"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="121"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00043952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="152" mass="17582" checksum="DD073F5B6957C5E1" modified="2017-01-18" version="1">MSIRQRILKEYRTLEYNKLPGIEVILTENLHQFYADLKITDNDLYPNTYRLKISIPEDYPFVAPVVVFVRDEDEGINEIPIHPHIYSNGHICLDLLGPGWVPVQTVSSLLLSIQSILASNDRNERPPDDERYCKGAPKNPTKTRFTYHDETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0B9</accession>
    <name>A0A1E3P0B9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_16241</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038118.1">
        <property type="nucleotide sequence ID" value="XM_019180682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0B9"/>
    <dbReference type="STRING" id="683960.A0A1E3P0B9"/>
    <dbReference type="GeneID" id="30197928"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061245">
        <property type="term" value="P:establishment or maintenance of bipolar cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244">
        <property type="entry name" value="KELCH REPEAT DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244:SF456">
        <property type="entry name" value="MULTIPLE EPIDERMAL GROWTH FACTOR-LIKE DOMAINS PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="376"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="546"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="833"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="624"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="729"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="381"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="427"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="438"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="899"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="899" mass="101936" checksum="105C5CD7F5C68994" modified="2017-01-18" version="1" fragment="single">NFKSPWTRSKLLISPFPRYRHAASSHANEKGEIYVMGGLHNTSVYGDTWILKPDLESRQFQSFQVDIYDNSPAPRVGHASTLCGNAYVIFGGDTVTNEAGEIDNDLYLFNMNSHAWTVPKPTGKKPSGRYGHTIGVIAITNFDSKLYLYGGQLDDVVYNDLCVFNLSSFRRPDVHWEWVHPSDHIRPPPLTNHSMDVYDNKLWLFGGSTGKKLTNELWCFDPQMERWDKIKTLGNQRPQPVEEHASVIYKDLMIVYGGKNSDGDALNDLYFLNLITKTWFKFPTNFPLEPQGKYGHTLSILKNDKLLILGGHLPDYANLGENLEVSNIDNGVGTILNLLDLTNLPKLVPGLQQYSTPNRFQKSHTPRQYSAEDVFQREQRQAPSSQNTPPHANRLNVPQTQPQQFSSPEEELPLQIPSPIKTENDVPGSFPSSGPSGIDRELKESREPSNQYPEVPKTQVEDLTKDIPGGFNDEFKTPNASPSKTSLNENGQHLQPQIYKNDEAKNSDFLDSYVNAPTSVNDNEEVKKIIASLSQELEDLKLSTNSQIKEASSKVRDLEEENSSLKKQLGETGESGDRDLDENSYKRKHIKLNTDYQILNKNHSQLKSKLDEIEPVFSNNLLDLQKLNNIIKQQSLTIEETQRSLSEQEDYKLKFSDLQGKYQALEESYKELSESRSKDFNETHGDVKQLSTNLDSFLAKYLVTDSEGATGTRSLSGSGVNNGQDDDVTTSLQAQIDELLKENEELHGQHKSLKDQYSSLESSQGGELEALRAKVTELSKIEENYKDSLHSVKNTSRALQVSQSELNKQKEVTLKLQQELDELKLFRANRKSSRNTTPIVNEFSKQETPKQPITTDEEEEDIESAHYNLKIRDLQAELFITKQERDELKHDVLELKKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0J2</accession>
    <name>A0A1E3P0J2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Peroxisomal hydratase-dehydrogenase-epimerase</fullName>
            <ecNumber evidence="5">1.1.1.n12</ecNumber>
            <ecNumber evidence="6">4.2.1.119</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Multifunctional beta-oxidation protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">WICANDRAFT_80566</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Second trifunctional enzyme acting on the beta-oxidation pathway for fatty acids, possessing hydratase-dehydrogenase-epimerase activities. Converts trans-2-enoyl-CoA via D-3-hydroxyacyl-CoA to 3-ketoacyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a (3R)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32711"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57319"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="1.1.1.n12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a (3R)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:26526"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57319"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="EC" id="4.2.1.119"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.n12" evidence="5"/>
    <dbReference type="EC" id="4.2.1.119" evidence="6"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037634.1">
        <property type="nucleotide sequence ID" value="XM_019185086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0J2"/>
    <dbReference type="STRING" id="683960.A0A1E3P0J2"/>
    <dbReference type="GeneID" id="30202332"/>
    <dbReference type="OrthoDB" id="3592703at2759"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003857">
        <property type="term" value="F:(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018812">
        <property type="term" value="F:3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03448">
        <property type="entry name" value="HDE_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05353">
        <property type="entry name" value="hydroxyacyl-CoA-like_DH_SDR_c-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000410">
        <property type="entry name" value="Peroxisomal multifunctional beta-oxidation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000185">
        <property type="entry name" value="peroxisomal multifunctional enzyme type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054357">
        <property type="entry name" value="MFE-2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051687">
        <property type="entry name" value="Peroxisomal_Beta-Oxidation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024">
        <property type="entry name" value="DEHYDROGENASES, SHORT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024:SF2">
        <property type="entry name" value="SCP2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22622">
        <property type="entry name" value="MFE-2_hydrat-2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0456">Lyase</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ketoreductase" evidence="21">
        <location>
            <begin position="9"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00055743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00073871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00081853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="ODQ58427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="889" mass="98155" checksum="2CFF5BB84156CCBE" modified="2017-01-18" version="1">MSELRFDNKVVVVTGAGGGLGRQYALEFGKRGAKVVVNDLGGSLKGDGTSSKAADVVVDEIKKAGGQAVANYDSVEFGDKIIKTAVEAFGTVHVVINNAGILRDSSFKNMSEKDFKLVYDVHLNGAYKLTRAAWPYFRKQKYGRIINTASPAGLYGNFGQANYSAAKSALVGFGETLAKEGVKYNIHANIIAPLARSRMTEDVLPKPMLEALDPIRVVPLVVYLTHEDSKASNQIFEVAAGYYGQVRWQRSSGILLRLDDTYTPEAILNQWNGIFEFEDKPFNKVTYPSHVSDFMTLIESQKSQPPNKQSKEKVSLKGKVVIISGAGAGLGRSHALTFAKAGAKVVVNDFKNPDDVVNEIKKAGGEAVGSKANVVTEADQIVKTALDTYGRIDILVNNAGILRDRSFGKMTDDEWFSVQDVHVMGTYRLTKLVWPIFLKQKSGNVINTTSTTGIYGNFGQANYSAAKSAMLGFSKTLAQEGLKYNIRVNVIAPHAETAMTATSFKENEFNKFDPSQVSPFYVVLASDHVKTTGETFEVGAGWIGNTRFQRSWGALSKDKNPSAEFVRDHFNEITDFTKFQLIKSPKESYLGIFERVGAEDEKDEDEDEDEEDTEVFKYTDRDAILYNLGLGANAKELKYVYEQHPDFQVLPTYGVIPCMSTNSLDYSGLLKNFDYNYLLHGEQYLKINKFPIPTAADLKTEAFPIAVLNKGKKAAIVVAGFKTFDKNTGEQIFYNEFTSFIRKAQAAKEPKVYKDRNSFATAANTIPSREPDFETTVKTSPDQAAIYRLSGDYNPLHIDPKVGTKAGFPQPILHGLASFGISAKQLYEKFGMYGEIKARFTNVVFPGEHLKVRAWKEGNKVTFQTLSVERNVVVINNAAINLVGDKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0L3</accession>
    <name>A0A1E3P0L3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_33395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038017.1">
        <property type="nucleotide sequence ID" value="XM_019182065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0L3"/>
    <dbReference type="STRING" id="683960.A0A1E3P0L3"/>
    <dbReference type="GeneID" id="30199311"/>
    <dbReference type="OrthoDB" id="40579at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003850">
        <property type="term" value="F:2-deoxyglucose-6-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051806">
        <property type="entry name" value="HAD-like_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43481:SF9">
        <property type="entry name" value="2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43481">
        <property type="entry name" value="FRUCTOSE-1-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="230" mass="25045" checksum="2293864A0E54F4AB" modified="2017-01-18" version="1">MSKKVTLNTDIVLFDLDGTLVYTTDVVEALWSEICDQYGVDKTNFFEHSHGSRTSESFAKFFPMIDNTDNKAVLKLENAIPDLHGDKVRVISGAIELLNGLDREKWCIVTSGNNYIAHSWFNSILKTVQKPTTFITAELVSKGKPDPEGYLKGTEILSKKLSLSSPKSIVFEDAPVGVKAGVAAGATVIGVSSGFDAQLLYDSGASYVVKDLTQVKVIDGDKITLEVAYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0L7</accession>
    <name>A0A1E3P0L7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rap-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_33156</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038226.1">
        <property type="nucleotide sequence ID" value="XM_019182038.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P0L7"/>
    <dbReference type="GeneID" id="30199284"/>
    <dbReference type="OrthoDB" id="19311at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033596">
        <property type="term" value="C:TSC1-TSC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032007">
        <property type="term" value="P:negative regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11210:FF:000007">
        <property type="entry name" value="Tuberous sclerosis 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11210">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000331">
        <property type="entry name" value="Rap/Ran_GAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018515">
        <property type="entry name" value="Tuberin-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027107">
        <property type="entry name" value="Tuberin/Ral-act_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024584">
        <property type="entry name" value="Tuberin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10063">
        <property type="entry name" value="TUBERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10063:SF0">
        <property type="entry name" value="TUBERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11864">
        <property type="entry name" value="DUF3384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02145">
        <property type="entry name" value="Rap_GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03542">
        <property type="entry name" value="Tuberin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50085">
        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rap-GAP" evidence="3">
        <location>
            <begin position="1151"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1391"/>
            <end position="1435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1400"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1415"/>
            <end position="1430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1461" mass="165863" checksum="047A1B5BE50512E6" modified="2017-01-18" version="1">MSSSQNVSTNNSNKSFGSGFLKSLASTFKTSNSDRNKIDASLITPDQQKQELFNEVRTGSLSSRAKAVEQISNCLYEFETSSIPDIWYCARDLISSNIQHSVRKSGLILLNACVDLDTEDSASRFAYFNDAILNCHFTKDKIDPEFESFFPIIKKLTDDGKFIHHHQESFKKPLVQFFINALSASELAKNKSVLPLLNYMNDCIKSGLLDVADFQQADTKDAELLLHQIMKLCLKTSDKEILELCLYSIDTLVSHGSIPEALYYETLEILAGSATLSLNFYDICCEVTTNFINEVEFQKVFQTLINIIQSKNSKSDKNITASVGAIKLVGFTTLSYAYQINMKDLLDALKTVISWNRNNINVAVLQFINDRLLSDTALSQISFQYWDSDDVSILGLLQDLSKNINKNDEIELYKLTLVHLGSLAESSRYFGNTEKLVRFFIDNNKYLSTKISVYVLKYFSLENLCSPLHEEWIKNCTIILNTFYYDHSKDLTVRLTTLKLVKDIFDSSLQVFKKDEAVLMKLGDFLFKSLESEENSEIIKTLVDYLIEISTSLSPDIYQNLLDVFILPGFNNSRERKSSLASFTSSDKSDQKKLFNKETGKLVAKSMATIFAKSLKSDGTKASMTYEALIKIANFSLAKKDVDLLLIVSRFFVRIRASTDKQIYLSNPTDMDGLSAAFSRNLNIRKKKEPEMSALSLDESEKWTYPEEITYIPQEFLDIPSDDLTIFDSGSFKLSLKPTIDIEKWLSVVIQILEACPHWEIYSFIWAHFCPQLSNIKLFETCGEGIRKLRSLACDQLSLKLPSNLKLPENVSKHDLQVAIVRNFTPLISYHDLFSKQDEDQIIGALVLGLSGWEKTAIPCVHILTVCCYEIPLSIKKYLSVILTKLQTRISSAFASAHILEFLLSLSYIPSLTSNFTVDEFKRAFGITFKLIQYAHEISETKNEQHQGILNHGQELAAENSPSTENLEVTPTISIYLLTLSYDVIANWYLNMKMTDRRQLSSFIIKNLIISQSNRGTDISHQNMAFLDLISRFTYSDLELKFSPINPQTFSSDDPVHSSKWVYGSSIVGIDTHAQTGESIISIRRASGSTVFRVTPDESMIPHFTNQLHAKEDPDDFTSNYMLLQLIVHTDPENTSKPIPIPEDPAITRSIANFDRIPVVEFHKIGLLYIGANQKTETEILSNSSGSLEYQKFLSQFGRLIKLKGCKSFYVGGLDTENDVDGEYAYGWNDKILQLIYHTTTLMPNQKDDPNFSAKKRHIGNNYVNIFFDESGLAFDFNVIKSQFNFLNIVIQPHSVSFGNDHKATENRIQKYKVKIHRRSGVPGLFATCHFKIVSEENLATFVRTLALIADQFAQIWHSNGNYASSWSHRMRQIGMIKDKAMKYHNEMKIKQNNDDSKSNEPSAKTGQSFLEQLSPSNNNSQVSRSNSISRPKVTNTNQYEYVDSEENQIFKNLEFTSFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0S8</accession>
    <name>A0A1E3P0S8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aminopeptidase</fullName>
            <ecNumber evidence="11">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_33117</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="11"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038021.1">
        <property type="nucleotide sequence ID" value="XM_019182034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0S8"/>
    <dbReference type="STRING" id="683960.A0A1E3P0S8"/>
    <dbReference type="GeneID" id="30199280"/>
    <dbReference type="OrthoDB" id="10031169at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000001">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF171">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="14">
        <location>
            <begin position="16"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="12">
        <location>
            <begin position="255"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="13">
        <location>
            <begin position="549"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ58814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="887" mass="99035" checksum="E82B6E7993DB0397" modified="2017-01-18" version="1">MSEAIPKRDVLPGNVKPVHYDLEVSNIQVNNNSFEGKVKIELDIKEDTDQIVLHALDIKFKQTELSVTKTESVVEIKQVSYDSKAQTATIQLGETLKAGPSIKAYLTITFDATLRDNMGGFYKSTYKDINGNDQVLLSTQFEAIEARTAFPCFDEPNLKATFSFSITVAEDYTALSNTPVLSSKVLDDGKKKGAVEASGLKLVKFQKTPIMSTYLVAWIVGKLDYVESTTERSYNGKKIPVRVYTSEGNSAKGKFGLEVATKVVDYFSEVFDIDYYLPKLDLIAVPTFASNAMENTALITFRASALLFDEASSDPKYKAKVAYVVSHELAHQWFGNLVTMDWWDELWLNEGFATWVGFLAVDKLYPEWNTFATFSSSTLQTALELDALRGSHPIEVPIQSASDIDQVFDHISYNKGGSVIHQLASTLGIDVFLKGVSHYLKKHQYGNATTNDLWSSVGEVSGVDVNSLADNWVRKIGFPYVEVKVENGNVNFKQNRFLSTGDVTEEENGTKWWIPLNISTGKEDSDRIKDLSFSEKEATIENLAKENSFFKVNKNSVGVYRVKYDDETFKTIVENIDKLSSTDKVGLIADSTVLSVAGLSKTSKALDLISAFKGESEYVTWLELIKRLGSITSVWFEQSEEVQAGLKKLAQELVSPTALSLGFESSSSQDFLTTQLRVELLSAAVSAGVPQIIEEAKSLFTKLQKNEEIDPSLRSVIFAAVISDPNATEEDFKFVYDTIKNSKALDSNEIALTALGTATNPDLIQKALNLILDPEIPIMDVSFISISLSSNKKARWAFWNYLKENYDAIYNRLNANRIVFDRFIKFTLGKFSSDEAYEEIKSFFASKNTYGFERSIDQVLDGIKTNSSWVSRDKDDVKEWLASRGYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0T4</accession>
    <name>A0A1E3P0T4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PDZ GRASP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_84619</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038135.1">
        <property type="nucleotide sequence ID" value="XM_019185744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0T4"/>
    <dbReference type="STRING" id="683960.A0A1E3P0T4"/>
    <dbReference type="GeneID" id="30202990"/>
    <dbReference type="OrthoDB" id="3318at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000183">
        <property type="entry name" value="Golgi reassembly-stacking protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007583">
        <property type="entry name" value="GRASP55_65"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024958">
        <property type="entry name" value="GRASP_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893">
        <property type="entry name" value="GOLGI REASSEMBLY STACKING PROTEIN GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893:SF0">
        <property type="entry name" value="GRASP65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04495">
        <property type="entry name" value="GRASP55_65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51865">
        <property type="entry name" value="PDZ_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ GRASP-type" evidence="6">
        <location>
            <begin position="44"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ GRASP-type" evidence="6">
        <location>
            <begin position="168"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="286"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="286"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="303"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="357"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="384" mass="42428" checksum="36F9A1813E7F9831" modified="2017-01-18" version="1">MFAFAQKFVKSIEQSAETIISGGANDESILENDAYFSNTKFNKYGLRVISVKPESKASELKFESWFDYIIGINGHEIPTGYNEQQQLVPNYEYLIKEIENCKNRSVIFEVWNAKGGVLKNIEVNIKELSSSSNLDDIPIDQKPNNIIQQSFEKIGVSIQWTPLITATFVYHVLEIHPNSPAERAGLIEHSDYIIGAQDGLLATGGEDLLGRLITSKKNQSLILYVYNHDYDVVRPVTIQPFENWGGAGLLGCGVGYGFLHRLPQAILNFEPGSILFDDNMTPNYTPNFSPSHNLSQESFNPVNPLPPVASIPPPPTSKQGSVSTPSPLSAGAPPPIHGRKKKQGAVKSDLLDYLQEEGEKSRELDNQGRPSHETDAALPPPPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0T6</accession>
    <name>A0A1E3P0T6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_31549</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038296.1">
        <property type="nucleotide sequence ID" value="XM_019181847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0T6"/>
    <dbReference type="STRING" id="683960.A0A1E3P0T6"/>
    <dbReference type="GeneID" id="30199093"/>
    <dbReference type="OrthoDB" id="438545at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005290">
        <property type="term" value="F:L-histidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015189">
        <property type="term" value="F:L-lysine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015194">
        <property type="term" value="F:L-serine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043937">
        <property type="term" value="P:regulation of sporulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF224">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="2"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="408" mass="44114" checksum="D8FA1A324BD96BEE" modified="2017-01-18" version="1">MASVQSSVINLTKTIIGAGLLAIPFAFKADGIVLGISLILIAGIASSYGLYIVGKVSAKVPGDTSYFALCAITYPKLTLLFDFSIAIQCFGVALSYLVLIGDLVPSLIGDVISRNQAILFSLVIIIPLVSFRKLDSLKIGSLIGLLAIGYLTVLVISHSIWDDWSKTQGHVKIVEIGSISDILSTFSIIIFAFTAAQNICSIINEINDKSRLNFVIGAANIIASVLFISVGIAGYLQFGDNVKGNVILEYKSKLLSSKVGKFALVLMVTLSYPLMFHPCRLSLNNMVHWIELEVQKEQSPINESTALLNDEEHNSNNQIVPFSTNRFVVITVLLTLATYGLALSITSFELVLSLVGATGSTLICFILPGLFGYKLFDDKDFNKSASLTLTIFGLVVMITSVFATLRYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0Z1</accession>
    <name>A0A1E3P0Z1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Malate dehydrogenase</fullName>
            <ecNumber evidence="3 12">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_79674</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3 12"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038353.1">
        <property type="nucleotide sequence ID" value="XM_019184931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0Z1"/>
    <dbReference type="STRING" id="683960.A0A1E3P0Z1"/>
    <dbReference type="GeneID" id="30202177"/>
    <dbReference type="OrthoDB" id="4069699at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01337">
        <property type="entry name" value="MDH_glyoxysomal_mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000013">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000009">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010097">
        <property type="entry name" value="Malate_DH_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01772">
        <property type="entry name" value="MDH_euk_gproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540">
        <property type="entry name" value="MALATE AND LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540:SF16">
        <property type="entry name" value="MALATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="13">
        <location>
            <begin position="2"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="14">
        <location>
            <begin position="152"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="8"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="122"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ59146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="342" mass="36741" checksum="FA526BB40E974235" modified="2017-01-18" version="1">MVKVTVLGAAGGIGQPLSLLLKLSSYVDELSLYDIRLSKGVATDLSHINTKVKVTGFEPSSKEDQSAIKQALQGTDVVIIPAGVPRKPGMTRDDLFNINASIIQSLATAIATTAPNAFVLVISNPVNSTVPIVAETFKQHGVFNPKRIFGVTTLDIVRANTFIKEYDSSLNGVEDVKVDVIGGHSGETIVPIYSTSEKGSKIFQKLSKDQKKALIKRVQFGGDEVVKAKNGAGSATLSMAYSGYKFAEALIRALKGERGVVEDSFIYLDSSLPGVSKVKQRTNVEYLSLPIRLGPEGVDTVESGILDKLDSTEVELFKIAVNTLEQNIKKGVLFANKLKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P157</accession>
    <name>A0A1E3P157_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Glutathione reductase</fullName>
            <ecNumber evidence="3 16">1.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_32786</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 16">Catalyzes the reduction of glutathione disulfide (GSSG) to reduced glutathione (GSH). Constitutes the major mechanism to maintain a high GSH:GSSG ratio in the cytosol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 21">
            <text>2 glutathione + NADP(+) = glutathione disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="13">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 15">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.7" evidence="3 16"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038426.1">
        <property type="nucleotide sequence ID" value="XM_019182004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P157"/>
    <dbReference type="STRING" id="683960.A0A1E3P157"/>
    <dbReference type="GeneID" id="30199250"/>
    <dbReference type="OrthoDB" id="5956163at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="Glutathione reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutathione reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006322">
        <property type="entry name" value="Glutathione_Rdtase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046952">
        <property type="entry name" value="GSHR/TRXR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01421">
        <property type="entry name" value="gluta_reduc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004776">
        <property type="entry name" value="PRK06116.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 13 15" id="KW-0274">FAD</keyword>
    <keyword evidence="5 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-0520">NAD</keyword>
    <keyword evidence="16" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 15" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="18">
        <location>
            <begin position="8"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="17">
        <location>
            <begin position="350"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="450"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="185"/>
            <end position="192"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="14">
        <location>
            <begin position="45"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012607"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00017111"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="RuleBase" id="RU365016"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="461" mass="50586" checksum="26B7BB102F24B257" modified="2017-01-18" version="1">MSPIPKHFDYLVIGGGSGGVASSRRAAQYGAKVLLIEGQHIGGTCVNVGCVPKKVMWYASDVAGKIAQSKEYGFDVDPNLSKTFNWEYFKPKRDAYIKRLNGIYERNLSKEGVELKYGWAKFNAKGEVEVHKSDGSDVETFTANHYLIATGGTPITPDVPGAELGIDSDGFFKLEKQPKRVGIVGAGYIGVEFTGFFHGLGSETHMVIRGDTVLRKFDDSIQTTITNHYEKMGVHFHKGSTVKSLEKLENDAIKVNLSTGDSFEVDQLIWTMGRKSLLGLGLEHTGVELEGDKIKVDEYQNATVPNYYSLGDVVGKVELTPVAIAAGRKLSNRLFGPEQFKNDKLDYENVPSVVFSHPEAGSIGLTEKEAIEKFGQDQIKIYKTNFTPMYYAMLEEKSPISYKLVCQGPEEKVVGLHLVGDSSAEILQGFGVAIKMGATKKDFDSCVAIHPTSAEELVTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1E2</accession>
    <name>A0A1E3P1E2_WICAA</name>
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        <recommendedName>
            <fullName evidence="6">Phosphoglycerate mutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_84847</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1E2"/>
    <dbReference type="STRING" id="683960.A0A1E3P1E2"/>
    <dbReference type="GeneID" id="30203041"/>
    <dbReference type="OrthoDB" id="354304at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045820">
        <property type="term" value="P:negative regulation of glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043456">
        <property type="term" value="P:regulation of pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051695">
        <property type="entry name" value="Phosphoglycerate_Mutase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517:SF1">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="2">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="17"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="24511" checksum="02C6F236210EABF9" modified="2017-01-18" version="1">MSEINTDDKVIRVFVVRHGQTNHNVAKILQGHLDTELNEQGLAQAELAGEKFKDYPIDAIYSSDLKRCKGTASTIQKHLSSPVDVTFSSNLRERNMGVIEGMKMQDAIEYAKKDGKSSYKEYGESSHALVSRIQKQVNKILEENKHHKNILLCSHGGTIRTLLKILKYNEDIIVYNTSVTIIDFNKEDFNDFKIIKIGDTKHLGEGEFKVSDTRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1F0</accession>
    <name>A0A1E3P1F0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_79685</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038365.1">
        <property type="nucleotide sequence ID" value="XM_019184933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1F0"/>
    <dbReference type="STRING" id="683960.A0A1E3P1F0"/>
    <dbReference type="GeneID" id="30202179"/>
    <dbReference type="OrthoDB" id="15688at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005848">
        <property type="term" value="C:mRNA cleavage stimulating factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="RRM_CSTF2_RNA15_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.70">
        <property type="entry name" value="Transcription termination and cleavage factor, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025742">
        <property type="entry name" value="CSTF2_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026896">
        <property type="entry name" value="CSTF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038192">
        <property type="entry name" value="CSTF_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735:SF2">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14327">
        <property type="entry name" value="CSTF2_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14304">
        <property type="entry name" value="CSTF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="8"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="89"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="261" mass="28657" checksum="3A0C610E51C11AF0" modified="2017-01-18" version="1">MSEKVPGKVIYIGSIPYDQTEEQVLDIAKSIGPVVNLKMMFDKETGKSKGFAFVEYKDVETASSAVRNLNNYSIGNRSLKCDFSNENSLSGSNGTGNNSGKGNNRSEDTLPPLPPGRSLRPGESYSDAISQTLQSLDPTRLNNLIRDVKQMSQKNPILMEELLAQCPQLSYALVETLLITQKASPEDITQILLGSAIQENQTNGDVPQVQEEEQIDEEKLALIKQVLEISDQELAQLPEDQRASILQIKENAQNGVYGGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1I2</accession>
    <name>A0A1E3P1I2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_84905</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038518.1">
        <property type="nucleotide sequence ID" value="XM_019185806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1I2"/>
    <dbReference type="STRING" id="683960.A0A1E3P1I2"/>
    <dbReference type="GeneID" id="30203052"/>
    <dbReference type="OrthoDB" id="1594986at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120171">
        <property type="term" value="C:Cdc24p-Far1p-Gbetagamma complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000935">
        <property type="term" value="C:division septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120157">
        <property type="term" value="C:PAR polarity complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000282">
        <property type="term" value="P:cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043577">
        <property type="term" value="P:chemotropism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072697">
        <property type="term" value="P:protein localization to cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007096">
        <property type="term" value="P:regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031106">
        <property type="term" value="P:septin ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05992">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13246">
        <property type="entry name" value="PH_Scd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010481">
        <property type="entry name" value="Cdc24/Scd1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033511">
        <property type="entry name" value="Cdc24/Scd1_PH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053026">
        <property type="entry name" value="CDC42_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47339">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47339:SF1">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06395">
        <property type="entry name" value="CDC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15411">
        <property type="entry name" value="PH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="3">
        <location>
            <begin position="239"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="438"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="4">
        <location>
            <begin position="664"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="626"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ59311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="746" mass="84816" checksum="7B1A7350E9909625" modified="2017-01-18" version="1">MLMNKQVTTSDSLYYICLRLKKRLESVSQLKPYLNLAYSSAEVLSENQALLLSQKSTTSSSSSNQHHSFNSSNGSNFRVSALSNFSSTSDDTSTNSNSTSQLNSPTSGAPGLINTFCAGVLPATVNCDPVTQLWALFQQGSPLCVIFNIIKPAYSLPVISSDDMKTCKKSIYDFILACKNYLNFDNSELFTISNVFSNETEDLIKIIKVITQVIDLAPSIFKNVDLTEEFKQAAAVTDSKSKVLKELVETERKYVNDLETLVTYKEQLLNFNIISSEDLYMLFPNLNDILDFQRRFLVSLEINNLIDPKYQRVGSVFIHAQSFFKSYEPWSIGQKAAIDFINTSSMKLKNNSLIIGNELELQSFLIKPVQRLCKYPLLLRELVKYTDPNTSNNYNELNLALEISKKIANDINENQRRVENVEVLKKLYDKVVDWKGYNVNSFGELLFFDKIVVKDNSKSSEEREFQVYLFENIIIFFKELLPQQKKSSIASLKKKSQTNLLSNVNQEALGFELKGRISIANIYNISTVNPYLLNISWSGMKDTGSFLMKFKNEEIRTNWESCIRKLVANLQDDYNNSNTSTLNNLNLNERRSNGISMGSNRSSYISRTNSESTTFQALSNNYQLHSRQQSEIPISSSKRSTQDSIQSTKRSISESYKNQLPNNSVIVKLMYEKDLFTLMTPLDISVDEFLSKIEKKIKQCGGVMKGKVKYQDEDGDFIVIQSDDDWSLVKEMMKEVGDKVLNVWVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1L1</accession>
    <name>A0A1E3P1L1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Glycosyltransferase family 62 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_31881</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ANP1/MMN9/VAN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038502.1">
        <property type="nucleotide sequence ID" value="XM_019181894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1L1"/>
    <dbReference type="STRING" id="683960.A0A1E3P1L1"/>
    <dbReference type="GeneID" id="30199140"/>
    <dbReference type="OrthoDB" id="3979765at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000017">
        <property type="entry name" value="Mannan polymerase II complex ANP1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052086">
        <property type="entry name" value="Mannan_Polymerase_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083">
        <property type="entry name" value="MANNAN POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083:SF2">
        <property type="entry name" value="MANNAN POLYMERASE II COMPLEX ANP1 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03452">
        <property type="entry name" value="Anp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="491" mass="56757" checksum="6BE1ADFC6DBBA750" modified="2017-01-18" version="1" fragment="single">MSRQRNIASSGGEGKPELSSSDEELQIDVKEKYNHHSQDSIFTSLNLDESSIIDGAFELFKLLIQTIYSVLPFFIIFYLYSIYQTNEEHFYAPDFPNSLHSSSIDSLNDIGVEFYDLADFKSTGNGWQNNERVLFLVPLRDASAHLPMFFSHMKNLTYPHNLIDLAFLISDTSDSTIPDLKSHLKTIQADSDKSKRFGRIDIYEKNFGQIVGQSFSDRHGFAAQGPRRKLMAIARNWLLTTALRPDHSWVYWRDADVETVPATIIEDLMHHDKDVIVPNIWRPLPDWLGSEQAYDLNSWQESEGGVELAEQLDEDAVIVEGYPEYATWRPHLAYLRDPYGDEEVEIELDGIGGVSILAKANVFRQGAMFPAFSFKKHAETEAFGKLCKTMGFNVIGLPHYVIWHIYEPSTDDLKHMEWLANEELRQREYEKNKVVYDKAWKLAFEDVSDQWREEKFNVFKNTDLSRLKIGSVDWDEVDEYLAEVDEFEDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1U3</accession>
    <name>A0A1E3P1U3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP synthase F(0) complex subunit e, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_79896</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Subunit e, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ATPase e subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038582.1">
        <property type="nucleotide sequence ID" value="XM_019184969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1U3"/>
    <dbReference type="STRING" id="683960.A0A1E3P1U3"/>
    <dbReference type="GeneID" id="30202215"/>
    <dbReference type="OrthoDB" id="2125027at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008386">
        <property type="entry name" value="ATP_synth_F0_esu_mt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05680">
        <property type="entry name" value="ATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4 11" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="105" mass="11402" checksum="99466CA75AA28DF5" modified="2017-01-18" version="1">MSTVNVFRYSALAAGVFYGAYHTYSLKSAATKAEEARVYAKKEALILEAKEKFAELKNKSQPKKAEAVTSAPASSSPAEINLDDDKLDFAKVILSAVENLEKAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P200</accession>
    <name>A0A1E3P200_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RFX-type winged-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_94880</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038698.1">
        <property type="nucleotide sequence ID" value="XM_019186470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P200"/>
    <dbReference type="STRING" id="683960.A0A1E3P200"/>
    <dbReference type="GeneID" id="30203716"/>
    <dbReference type="OrthoDB" id="338531at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009303">
        <property type="term" value="P:rRNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052406">
        <property type="entry name" value="Chromatin_Remodeling_Comp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22970">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22970:SF14">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="505" mass="57725" checksum="8D1DC2B4409C1FE4" modified="2017-01-18" version="1">MSVPQLPQITPTAIIVPSSDQQQSSIYNNYAQLNQNLFSHPGSNFQGSDLMTHLQLNLKAGINLDWCSRTILHFSNSAPYLVKHQWILDYLLNDLYDNFNKTSALSLRNLSQDGDFTQLLALHPKTKKILYEVLSHFETDDQPSPLIDDVENTSKYSNLDNELLMYTVDIVESVSSYIAPAPKDDDLFVCLTKIFQTHNDRSLLISIMRSFARFLVRSELSVENCSSNLTSNILDKIVSFLLTNDYDLILTSLDILYQFALPGNLRINALLKSSTRQEILKLKLPQLLTYQLNIKGNPNDFSQLDYLKLIKRTKPPMPTSPPVLSPEHYKDIIILNEPLRATSWMRCSYKAVEDGEVTQISLWKAYEKQFEKEAVQNKKNYYQLAMVLNLPSGQRKFIIKGIEPRFQSVDISTGEYEAFNQIEKSQNHDLNLLNPLDSKQEPLPIYNPPQQLNDVNKSSSMLLTSLTNNSIGKELFKPVEEEIFKKIEAAPVLFDELADSLKYLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P213</accession>
    <name>A0A1E3P213_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">thioredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="3">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Nuclear thiol peroxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Thioredoxin peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_30192</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peroxiredoxin family. BCP/PrxQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="3"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038736.1">
        <property type="nucleotide sequence ID" value="XM_019181697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P213"/>
    <dbReference type="STRING" id="683960.A0A1E3P213"/>
    <dbReference type="GeneID" id="30198943"/>
    <dbReference type="OrthoDB" id="338622at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03017">
        <property type="entry name" value="PRX_BCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000157">
        <property type="entry name" value="DOT5p Nuclear thiol peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050924">
        <property type="entry name" value="Peroxiredoxin_BCP/PrxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801:SF23">
        <property type="entry name" value="PEROXIREDOXIN DOT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801">
        <property type="entry name" value="THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="15">
        <location>
            <begin position="52"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ59529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="198" mass="21909" checksum="E46712D7FD28CE07" modified="2017-01-18" version="1">MTQLRRSARVASKKPESETKIESLKTSKVTKPKKEVKKKDLKEVSATESKELEVGDEIPDITLLNQDEESISLKGAASKNKIILIFAYPKASTPGCTRQACGFRNNFNDLKEKALILGLSADSPKAQKNFQSKQGLQYDLLSDPKKELIGVLGAKKSPSGIKRSHWIFKDGKLAVKRVQISPEVSIEDGEKEVIELSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P232</accession>
    <name>A0A1E3P232_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">DNA-directed RNA polymerase III subunit RPC3</fullName>
            <shortName evidence="8">RNA polymerase III subunit C3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_52808</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 8">Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038728.1">
        <property type="nucleotide sequence ID" value="XM_019182699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P232"/>
    <dbReference type="STRING" id="683960.A0A1E3P232"/>
    <dbReference type="GeneID" id="30199945"/>
    <dbReference type="OrthoDB" id="272392at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055207">
        <property type="entry name" value="POLR3C_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013197">
        <property type="entry name" value="RNA_pol_III_RPC82-rel_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008806">
        <property type="entry name" value="RNA_pol_III_Rpc82_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039748">
        <property type="entry name" value="RPC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949">
        <property type="entry name" value="RNA POLYMERASE III DNA DIRECTED -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08221">
        <property type="entry name" value="HTH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05645">
        <property type="entry name" value="RNA_pol_Rpc82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20912">
        <property type="entry name" value="RPC3_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22536">
        <property type="entry name" value="WHD_POLR3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase III subunit RPC82-related helix-turn-helix" evidence="10">
        <location>
            <begin position="15"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase III Rpc82 C -terminal" evidence="9">
        <location>
            <begin position="151"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase III subunit RPC3 winged-helix" evidence="11">
        <location>
            <begin position="425"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ59521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="586" mass="66241" checksum="6F51922F17090145" modified="2017-01-18" version="1">MLASESAKSQSPESFVYTGLIESYLGKRAALISSVLISYGRLSARELSKKSKLSPSTVKKTLVSLIQLNCVSVWVETTLKSETTYYYFKEDGALALLYSGEIIGYVDKLYNNDSLTQIVSNFLSLGNLSINDYLSSFGSDDKEEVHQLEKHFASLVTDKFLIPISKTHFNPLKDLWLQTYKKAYGRIPKTSTLSELKRKAEAKAFAKLEFLKIVDYQPESLIIKDKSTSSSKINPNISLSFNIQRFLKYKRSVQLTHFCCHRIGKVTSVIYESALLATDKNTPPVINPLHQIGLSNEEFEDASAYEPKNSCTFNARDVLKHLPTDFILNGTILNGPTKRKASDSDTRSSSKRVKLEEGFSVPELNGEDHSDDEVEDSNTAKLAIVEQHLKILTNSTIPFLKKAQNGLYYVPFPEVLTLLKRSVYDSVISSTLGAPCARILRCVRDNRLVSEKLINSVALLREKDARSLTATLVKYNSLQIQEIPKSADRAASKSVFLYRFNEKHSFDFLKSNVCWNMGQLLEKIDCLKQENSGLITKINRDDVKGKEMDYLLPSELTQLKELKDRELDGVIKINRLTSIWEVFKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P269</accession>
    <name>A0A1E3P269_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Retrograde transport protein Dsl1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_62164</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038783.1">
        <property type="nucleotide sequence ID" value="XM_019183318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P269"/>
    <dbReference type="STRING" id="683960.A0A1E3P269"/>
    <dbReference type="GeneID" id="30200564"/>
    <dbReference type="OrthoDB" id="534815at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021876">
        <property type="entry name" value="Dsl1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021875">
        <property type="entry name" value="Dsl1_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046362">
        <property type="entry name" value="Zw10/DSL1_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11989">
        <property type="entry name" value="Dsl1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11988">
        <property type="entry name" value="Dsl1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrograde transport protein Dsl1 N-terminal" evidence="2">
        <location>
            <begin position="46"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Retrograde transport protein Dsl1 C-terminal" evidence="3">
        <location>
            <begin position="503"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="334"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="683" mass="79928" checksum="F55EB5CD2B31AA4F" modified="2017-01-18" version="1">MEVIDGDLDLRIDQLNQKIDGIIDPANLRAQVENVGGVSKALSFDELSKLQQRDYEITQELQQLRELWVISTLLKETEVNLELFEFENVFDSLRNFQKKIKSDSLTSSLIIVDKLQNEHDRCYDLAIEKIKQCWNELIHVDNDSIEFNAEVEINGNLVQFENISDVIKTNNLQTKIPNSNTSHLIDQKLLNSLTESKSLNLSDNKISINNDHESIQDQIQSISNLVKFISFIPDDQQIINYISPRFFEWLKELITINIDLVYSDKSLQNQFLELDSMLKSQNFKRNDLQNWIKNELNEIAVENYLDLHIDQARKLVYSLDKSVFQNLMTREYKESVESKPAPKAEPVPQPPVDEPEDDWGWNDEDINVDDDQDPQQEEVQDDWGWDDDEEEQPKPKKKLVSKLAKKSHSNEPSRESTPIPQTSITYQTTKLPEEVNKIIKSYLSTQSKLPSQYHDLHYSKLNYLITGFYILISQEFKTRTKSSLLLYNDLTYLNQLSKFSRIVELKEQYLSNYIKDFQNDIDEHYSKLQGLDPSISSNETYKIIDDIQLIFTMFFKKLEIIPSSKYQEILYSAIESFYNQIIESVFAKTNIGEIESEYLNLILTRFLQLKFPVDHNKLKNHSKLQHVAFIVNSHLKDIMESFYNAEFYDLSTMELIALIDKLFADSDLKRRSIEDIREIREAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2C8</accession>
    <name>A0A1E3P2C8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_104643</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038762.1">
        <property type="nucleotide sequence ID" value="XM_019180423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2C8"/>
    <dbReference type="STRING" id="683960.A0A1E3P2C8"/>
    <dbReference type="GeneID" id="30197669"/>
    <dbReference type="OrthoDB" id="5572782at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133647" description="Fibronectin type-III domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="40"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="157"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="469"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="569"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="689"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="300"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="174"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="471"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="495"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="569"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="696"/>
            <end position="763"/>
        </location>
    </feature>
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        <location>
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            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="796"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="873"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="918"/>
            <end position="931"/>
        </location>
    </feature>
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        <location>
            <begin position="946"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="989"/>
            <end position="1017"/>
        </location>
    </feature>
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        <location>
            <begin position="1019"/>
            <end position="1033"/>
        </location>
    </feature>
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            <begin position="1054"/>
            <end position="1063"/>
        </location>
    </feature>
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        <location>
            <begin position="1064"/>
            <end position="1094"/>
        </location>
    </feature>
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        <location>
            <begin position="1112"/>
            <end position="1124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ59555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1124" mass="124846" checksum="5349318FCE934ED9" modified="2017-01-18" version="1">MYWTLTVLSLACLWLAHRLYVLAKIPVPELIKTLNIDIPKASKLSVDNITCSKVHIHWDLPLLEHKIVSFVLYVNGKQASTLNGNVSQCCLSSLFPNTKYKIDLISINSNGYKAKSESIFIKTKPEKLQDYNILLENPENLFRLLTDNPDVSTVHKLTTPSTITSNGEARAAGRSRSNTVNSVNGNGTTAPQTNGLASYAASTATNNSFLPNPKSMDDIEELRYYLESGQEELHTILNQQTQALKDFKEQEYILIEERDKLRDRKKLEDTNRQAIKTEMNLLDDSRRLTELKKSKQESSLIAKRKNIQKMESDLNNWNLKIDEFEKHKNDLTESEDRVHSEIDSDIAIKKSKIKELQDAISKIDEDIKAINSKKKQKEQLKPQFIKIFKTLGDHTDIGGCLDKEGTKTLEQLKSLDIEVHQKIQKEIESDARLEAEWRAQQQREVNNCLRISGVKEALKRENVNLKVALNPSSSHSSTPPSVHSQPSNLVASQIPQHQQNQSSLHQPQQRDISPPNPNAFPFAVPNAFNYNNVQNGSSPSLSSYQVTQNSTPPAFTSSSLWNTFTIPNGSNQGNSSDGFAFNTTLPNNGTLQRPEFEPEVDDSEVLPNAETSAQHLLPKYLIDEDNDFMNLVKGVQGDLSLENSSNSNHFQQGPNSHFGMNNAFSNVHNDSSANLSTKSIFHNEFGIPTQPRFSLDNPSSPPQSFNAELLSNQTSPSNANNNDIHSVFSGNHNELLQNSFQPQPPTQVAQQSSKDTNHISSFSPRRLSNVFSFGRRNNDVNDNQNNNTNEPPASAPSLSKSQQQHSKFFNKNAVTSNANNGDITGTPQSHAHSGSNPLFTFSPEVSNQKNSTPSKFILDSVWKSPGESDNSHNRNVSMNSAHSNNTSFEKEQSFSKFFPGPSVQNNDFLTAPTKESHNNNIGSNVSFSSDAMSDHVNMETGGPIQLSATNKSSNTTSIKSLKSSADNASNVSSSPSFFRKNKFLMNPFGGNNNQANELGNKSPSKQHSTLFRTSSPTKIDAHNTEDSDIEEFHPGSNNNSATTPKRLFSRNRKSSSSNIRKQSFASSHNNDTDSNYNATDGSIQSTNTSASNGKSLVKKFFGKNKGEPKENDLDEQIIEEDIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2R8</accession>
    <name>A0A1E3P2R8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_78334</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ59695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038902.1">
        <property type="nucleotide sequence ID" value="XM_019184705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2R8"/>
    <dbReference type="GeneID" id="30201951"/>
    <dbReference type="OrthoDB" id="410267at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF177">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER MCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="346"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="384"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="420"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="5">
        <location>
            <begin position="255"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="453" mass="49901" checksum="5E4C58A813A72988" modified="2017-01-18" version="1">MNDPEKQSNRVHVGEADEEEFPEGGKGWLVLLGCGLTSIFVFGMINSYGVFQTHYETVLFPNVSSSKLSIIGATQASLCYFLTPWTVPLVHAFGLRQVLSVGASFLIISFFGLATTTPSQLWKCYIFQGLFFSIGAALQFGPAMTVPSEWFKKKRGTAFGISAGFVGVGGVVWPILFKQMINKHGFKWTVLTIAFVYIPLSVGSILLVPQHLEDRYNHKGKCFSNAVWNNENIKNLPQSFKNILIEWSHVIKNPRYSIILLANLLVSFGSYPVIFYIDFFGHTIGGEAKIVSYLTMIFVVMGFPGRAIPAMIADKIGRINVLIVCSAVLFISIFALWIPSIQYNLLGVYIGFVIVFGFAVGPLFSLFPASLGQLFGIRGNEARLSLFLFSATPGPILGCLIAGSFIPIDSTDTEKIVHSFYKVVIFSGIILTVCTVLLTGVRLSISRKPFVFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3H7</accession>
    <name>A0A1E3P3H7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_29914</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3B4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039110.1">
        <property type="nucleotide sequence ID" value="XM_019181665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3H7"/>
    <dbReference type="STRING" id="683960.A0A1E3P3H7"/>
    <dbReference type="GeneID" id="30198911"/>
    <dbReference type="OrthoDB" id="10259687at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048026">
        <property type="term" value="P:positive regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12334">
        <property type="entry name" value="RRM1_SF3B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000895">
        <property type="entry name" value="Hsh49p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000505">
        <property type="entry name" value="Splicing factor 3B subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034158">
        <property type="entry name" value="SF3B4_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052084">
        <property type="entry name" value="SF3B4_spliceosome_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030:SF3">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="14"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="104"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="210" mass="24282" checksum="D50E1F2014453571" modified="2017-01-18" version="1">MLKRGIESDRNQDCTVYVGNLDEQVNEGLLYELFLQIAPIKNIHLPKDRILRTHQGYGFVEFKNVKDVEYAEKVMSGTKLFGKNIKVNKVGNNAANNDNLDIGSTLFIKNLDELVDENLLFTIFKQFGNFFKNPTIVRDDQGNSKKHGFIYYKNFKDSDNALSKMNNQFILNKRVQIDYAHKKKNGKLVKHGDETERLLLTEAEKNNYNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P406</accession>
    <name>A0A1E3P406_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase C-like 1</fullName>
            <ecNumber evidence="2">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">WICANDRAFT_29827</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="2"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038839.1">
        <property type="nucleotide sequence ID" value="XM_019181656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P406"/>
    <dbReference type="STRING" id="683960.A0A1E3P406"/>
    <dbReference type="GeneID" id="30198902"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000935">
        <property type="term" value="C:division septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032186">
        <property type="term" value="P:cellular bud neck septin ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902660">
        <property type="term" value="P:negative regulation of glucose mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000425">
        <property type="term" value="P:pexophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903139">
        <property type="term" value="P:positive regulation of cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010606">
        <property type="term" value="P:positive regulation of cytoplasmic mRNA processing body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090334">
        <property type="term" value="P:regulation of cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000769">
        <property type="term" value="P:regulation of establishment or maintenance of cell polarity regulating cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070610">
        <property type="term" value="P:regulation of fungal-type cell wall (1-&gt;3)-alpha-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060211">
        <property type="term" value="P:regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034063">
        <property type="term" value="P:stress granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd20822">
        <property type="entry name" value="C1_ScPKC1-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20823">
        <property type="entry name" value="C1_ScPKC1-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08689">
        <property type="entry name" value="C2_fungal_Pkc1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11620">
        <property type="entry name" value="HR1_PKC-like_2_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05570">
        <property type="entry name" value="STKc_PKC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000101">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000103">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000014">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000034">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037778">
        <property type="entry name" value="C2_fungal_PKC"/>
    </dbReference>
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        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037312">
        <property type="entry name" value="PKC-like_HR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
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    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
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    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02185">
        <property type="entry name" value="HR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM00742">
        <property type="entry name" value="Hr1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
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        <property type="entry name" value="REM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16 18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="REM-1" evidence="24">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="24">
        <location>
            <begin position="109"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="20">
        <location>
            <begin position="192"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="22">
        <location>
            <begin position="404"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="22">
        <location>
            <begin position="471"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="698"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="23">
        <location>
            <begin position="958"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="66"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="324"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="568"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="620"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="327"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="337"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="570"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="646"/>
            <end position="663"/>
        </location>
    </feature>
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        <location>
            <begin position="664"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="727"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022741"/>
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            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
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    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="ODQ59632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1025" mass="116907" checksum="D2802FA935DBA84F" modified="2017-01-18" version="1">MSDSVSEDIKKKILRERNIINGAANLKQKTTNPAVIQKCNSNIQEAQQNIDYLSETLNQLRFQEPVTIGSGPTTESIGGDNANENGASLSKKPSASPDVGNYSRLDLMKYDCPSLGQRIQYMINQLNTKLKVEQKYYEANEKLSKIYQLDGDRRISSVAEDGRVQSNHKIQLLKKSLKRYEDIHVNLDQVTPETDPMNNVKFRRKPLGGTLTIGISSLRDVDHIASPLFSRKTESYISIKVDDDEKAKTKGSRNDRWNEEFNIHIDKGNEVEVTVYDKIGNQHVPVAIAWFLVSDIAEEIRKKKVGQSGVGWVSASRIQNGRRVANPNVSNDNFTLNEKESPNSENSRDPIRSSSWYILEPAGQILLTIGFTKSTTSNKKTLLGGLHRHGAIRKRDEEIYEQHGHQFAQKQFYSIMRCALCGEFLPYNGYQCQDCKFLCHKKCYQKVITKCISKSSTDIDPDELKLNHRIPHRFEHVSNRGTNWCCHCGYILPWGRKNVLKCSECDTMCHSQCSHLVPDFCGMTMETANKILSTIKSTEGSRKKQIQTYLSSQSSATTAVNTIDEKETLPSIQPSYQTLEPPQTQPRLPSLPPSRQVEEERLLQEQQFAEERRRQLLEEQNLRQQQQQQEYEEQQLSNRQSLHSELSGKLDPRHSLDSDKSHEGSTQADSQYQEVSSKSDKSHHSHKRHRPKVGLDDFNFLAVLGRGNFGKVMLGRSYRTKQLCAVKVLKKDMIIDNGEIASMHAEKEVFLVANREQHPFLINLHCCFQTANRIYFVMEYVPGGDLMWHVQKATFSQRRAKFYAAEVLLALKYLHENGIVYRDLKLDNIMLSVDGHIKIADYGLCKPDMWYGKTTNTFCGTPEFMAPEILREQHYTRAVDWWAFGVLLYQMLLGKSPFRGEDEDEVFNAILTDEPLFPIQMASEAVGLLESLLTKDPNVRLGSGPRDAEEIMEHAYFRNINFDDILNLRVPPPYIPEVKAEEDVSFFDKDFTTEVPRLTPVNSVLNATNQAQFRGFSYVSDSSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P445</accession>
    <name>A0A1E3P445_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tryptophan--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Tryptophanyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_30156</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="3"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019181693.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3P445"/>
    <dbReference type="GeneID" id="30198939"/>
    <dbReference type="OrthoDB" id="15808at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070183">
        <property type="term" value="P:mitochondrial tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000082">
        <property type="entry name" value="MSW1p Mitochondrial tryptophanyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000002">
        <property type="entry name" value="Tryptophan--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050203">
        <property type="entry name" value="Trp-tRNA_synthetase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43766">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43766:SF1">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ60259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="370" mass="41622" checksum="396E66EEF6212A41" modified="2017-01-18" version="1">MLSVLRRSQLLKSCATRQIRCNSTVKTTSYDPKDLSIPEHSSIFSLIQPTGKFHLGNYLGAIRVWNDLCEKAPDSSRVIYGVADLHAMTIPKPAKQLKTYRYEAIASILSSGIDSDKAIIYHQSAVSEHSELYWILSCHAGVGYLNRMTQWKSKSEGLDSASLGLFAYPVLQAADILLYKSTHVPVGDDQSQHLELTRHLATQFNKHFKKTFPIPTTILAPTKKILSLKDPSKKMSKSDPDQNGTLYVNDTPEVLQKKIKKAVTDSISTHFNYGPINRPGVSNMINTISGIQRKSIEEVERDIAHFNDHQTFKNYVTDVITEEFTKPRKLFDELMKDPEYLHSVVEKGNSKAREIASKTLKEVKKNAGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P462</accession>
    <name>A0A1E3P462_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_78891</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039493.1">
        <property type="nucleotide sequence ID" value="XM_019184804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P462"/>
    <dbReference type="GeneID" id="30202050"/>
    <dbReference type="OrthoDB" id="3980402at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="270" mass="31784" checksum="990528A3A18E0ABF" modified="2017-01-18" version="1">MSRLSKINLESPNAVGSFVQFARLLSNPLVYDKINDFITNNKDSVFDIVQYSAILISEIIRNYPKLKHLIRKLLFRFKLIKVESNEKVDDQEPQLTETQQYYSTVFRSMYGYISDIRIAQGIFAIFPYISTFLKDYNSLEKLKNINTKDKLFDFTTWAIFAIYTVFENLGFLLSHDFIHDNFNHLLLPQHRKDKGLSSSDWYYVYSCRFWCLWNAIEVLKDLKNGVQFNLLCDLILSYHWSTTTGALDKTSFAIIGFANSFTKFRRLLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P471</accession>
    <name>A0A1E3P471_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_105248</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039488.1">
        <property type="nucleotide sequence ID" value="XM_019180442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P471"/>
    <dbReference type="GeneID" id="30197688"/>
    <dbReference type="OrthoDB" id="3980805at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="152"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="176"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="215" mass="23948" checksum="5E8D6BA6846ABD35" modified="2017-01-18" version="1">MTLPQASAVPITNKGSLETITLKNLVALDLIESNENLLKAFRLFDDSKTKISLGDNLTKEQLQNLIKQKQNEINTQQDELKTLKHQISQDMDKDNQLINSLASEFLKDEIAQDDTDQLIAEFEEKNDVKVQFNENGDHFKEFSLDLGINAATKPKDESTDSQIPEQSSQPFPPSSNATIQVPTSAPESTTINNDTTQPPSEQAASQQDNDTVMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P475</accession>
    <name>A0A1E3P475_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ferric-chelate reductase (NADPH)</fullName>
            <ecNumber evidence="4">1.16.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">WICANDRAFT_31099</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>2 a Fe(II)-siderophore + NADP(+) + H(+) = 2 a Fe(III)-siderophore + NADPH</text>
            <dbReference type="Rhea" id="RHEA:28795"/>
            <dbReference type="Rhea" id="RHEA-COMP:11342"/>
            <dbReference type="Rhea" id="RHEA-COMP:11344"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.9" evidence="4"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039496.1">
        <property type="nucleotide sequence ID" value="XM_019181799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P475"/>
    <dbReference type="STRING" id="683960.A0A1E3P475"/>
    <dbReference type="GeneID" id="30199045"/>
    <dbReference type="OrthoDB" id="4494341at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF9">
        <property type="entry name" value="FERRIC REDUCTASE TRANSMEMBRANE COMPONENT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-0408">Iron</keyword>
    <keyword evidence="17 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="22" id="KW-0732">Signal</keyword>
    <keyword evidence="9 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133656" description="ferric-chelate reductase (NADPH)" evidence="22">
        <location>
            <begin position="19"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="23">
        <location>
            <begin position="427"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="622"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="626"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="ODQ60289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="717" mass="81678" checksum="923BA84F38E229E9" modified="2017-01-18" version="1">MKLTSILSWLHLFIIVSAVEKFRYSHDELVVIGCKYALTHTALFCEDDNDQSYSCQCLDPAAKASLMNCIYEKTSINNTKAEYILIQYCENLNVTLDSADLRQAYKNGTKYMVKTTDISGFNISSVIKSPIDHYNSTAYDSAYGTYWAFYGMIADSIYFGGGIMGYWAVIFLGAALANLTSKMAFASGHFNYSLVNKIRKNITMPAMFSNKKHTQAVYPFGRRLSMLSGLLPTRGESIVIFGFFLLNVIFEGARYHFVQRNTFFPNQTSQMSVYIGIRAGILALYGFQLTYLFAGRNNFLIWLTGWKQSTFITYHKWISRFNFLLIVVHAASLHIQSKASGRFEQRIATHWYKWGIVAGIVGGIIMVSAIHWTRTHYYELFLLIHIAMAVLFLTASWIHIHNFKYSEFAYSMAAIWAFDRAVRIGRLFSFGVRTATVTVVSDETLKIRIPKPKYWNAFPGSFGYIHFLRPTTFFQSHPFTLVSDNENEVCVYAKIKGGVTSQVHKDLASQSNKTGKIKVTVEGPYGDPKPMKHYDTCLLYAGGNGIPGIYAYAKKLAQISNKHIKLYWVIRNWHSIDWFYEELLTLKSLPLDVIIYITKADSSLGSKFGSISSESYFNEKEDASINDMSQRNSNKKLPQDSTPHEIVSRYLDFVEFRFGRPNIDQLIREDLEEAEENSVGVMTCAHNAMVDGIRDAVSQNLDATKGRVDYFEELQTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4B8</accession>
    <name>A0A1E3P4B8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">OBG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_29866</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in ribosomal function.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038939.1">
        <property type="nucleotide sequence ID" value="XM_019181661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4B8"/>
    <dbReference type="STRING" id="683960.A0A1E3P4B8"/>
    <dbReference type="GeneID" id="30198907"/>
    <dbReference type="OrthoDB" id="603at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17230">
        <property type="entry name" value="TGS_DRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000003">
        <property type="entry name" value="Developmentally-regulated GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000740">
        <property type="entry name" value="Putative GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1070">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="65"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="5">
        <location>
            <begin position="291"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00059727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="368" mass="40541" checksum="333811C1B5CC63FE" modified="2017-01-18" version="1">MSTTVEKIKAIEDEMAKTQKNKATSFHLGQLKAKLAKLRRELLTPSGSGGGGGGLGFDVARTGVGSVGFVGFPSVGKSTLLSKLTGTHSEAAAYEFTTLTTVPGIIRYKGAKIQMLDLPGIIEGAKDGKGRGKQVIAVARTCNLIFIVLDVNKPLEHKRIIEKELEGFGIRLNKEPPNIIVKKKEKGGISITNTVPLTHLDNDEIRAVMSEYKLNSADIAFRCDATVDDLIDTLEASQRKYIPAVYVLNKIDSFSIEELQLLTRVPNAVPISSGKEWNLDELLEIMWDRLNLVRVYTKPKGKLPDFNEPIVLRSNRCTVEDFCNQIHKSLVSDFRTAVVFGSSVKHQPQYVGLNHVLEDEDVVTILKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4L9</accession>
    <name>A0A1E3P4L9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_79020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039628.1">
        <property type="nucleotide sequence ID" value="XM_019184821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4L9"/>
    <dbReference type="GeneID" id="30202067"/>
    <dbReference type="OrthoDB" id="3969899at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="113" mass="13333" checksum="E2378B3C8731A8CE" modified="2017-01-18" version="1">MLRSQLIRASSINKKIYIKPIRFYASSSPSDYLKWKELKDDDKRDFISKFVESYRSKHKNTKNYYKQLASDMDVHGDIPSVFGLLYNDLIEKSVNNEVTSEFDADFFGLLYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4M9</accession>
    <name>A0A1E3P4M9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycosyltransferase family 34 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_84115</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 34 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039381.1">
        <property type="nucleotide sequence ID" value="XM_019185651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4M9"/>
    <dbReference type="STRING" id="683960.A0A1E3P4M9"/>
    <dbReference type="GeneID" id="30202897"/>
    <dbReference type="OrthoDB" id="407658at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007114">
        <property type="term" value="P:cell budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008630">
        <property type="entry name" value="Glyco_trans_34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306:SF5">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05637">
        <property type="entry name" value="Glyco_transf_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="385" mass="45392" checksum="C7B60F064263A006" modified="2017-01-18" version="1">MSQLQRYFSRIKNRATANIYLTLTIAILSLYFVKTVLIDEDSSYLFDLSDGPGTSGFGITKGGTGNQGYQKDHRTGGLPPTARKIYENKDITTYEEYGIDGNQQHNEFFNKDKNKIIIILGANIEGGVNKWKGPNEWSIERSSILNKKHYAKKHGYELIIKDYTKSKKYSNDYREGWQKFDIIKHAFDQYSSGDWFWYIDLYTLVMEPQISLENLIFKKINETIERDLNYFNPNQLELDVPFTNYDEPLNLILTQDCGGFNLQSFLIKKTEWSITLLDILFDPVHYLQNIATWKNGEKNALEYYYNKFGWIRSRVGFLPTRTISSLPKGACPDFEGDERFFYNESSRDFLVNMMGCEYNRNCWDEMEFFKQLSKDLHEKWYQKWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4U6</accession>
    <name>A0A1E3P4U6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Luciferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_29768</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the NtaA/SnaA/DszA monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039590.1">
        <property type="nucleotide sequence ID" value="XM_019181648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4U6"/>
    <dbReference type="STRING" id="683960.A0A1E3P4U6"/>
    <dbReference type="GeneID" id="30198894"/>
    <dbReference type="OrthoDB" id="5561043at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051260">
        <property type="entry name" value="Diverse_substr_monoxygenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016215">
        <property type="entry name" value="NTA_MOA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03860">
        <property type="entry name" value="FMN_nitrolo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011">
        <property type="entry name" value="ALKANESULFONATE MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011:SF16">
        <property type="entry name" value="C2H2 FINGER DOMAIN TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000337">
        <property type="entry name" value="NTA_MOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="6">
        <location>
            <begin position="41"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="497" mass="55994" checksum="E8F4168F2144F81A" modified="2017-01-18" version="1">MTQPTSKKQKVAHSKKTWIINAAGNASAGKWRHPQDRSSELGYSIDAWIEYAKIAERGKLNAIFAADHLALFDNYKGPNNYQLPAKEGFAIARIDPFSLVSALATHTKNIGFGITASTVSEHPYHFARRLATADNLSKGRVGWNIVSSYLSGVGRQLLNGELPDHDERYVKTTEYLDVVYDLLLSSWRDGSFVNDPKRGVYVEPDGIREINHTGKYFNVPGPANFHYTPQRFPLIIQAGSSSKGIEFAAENAEVVFLNSHDPNIIAKSVANVKKLAKEKYGRDPNHLKCLVPANVILGKTNEEAIEKLKLYEKYTNEDAQQASFSSVTGIDLSKFELDDVVTIDKKSNAMRSATDSLLNKGQKLHTKREILKTYNQYGLFGKAFAGTAKEVADQLEQWVNDYDIDGFNFGFGAVFPQFLEDIVDLLIPELQERGVFWKDYPVNGGTFRENVFGVKGQNFLHEDHPAYKLRWEAGISKEQFEKNLEEYQKIRLGRREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4U7</accession>
    <name>A0A1E3P4U7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">6-phosphogluconolactonase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_84296</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May be involved in regulation of tRNA subcellular distribution.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039642.1">
        <property type="nucleotide sequence ID" value="XM_019185684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4U7"/>
    <dbReference type="STRING" id="683960.A0A1E3P4U7"/>
    <dbReference type="GeneID" id="30202930"/>
    <dbReference type="OrthoDB" id="432544at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01400">
        <property type="entry name" value="6PGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000017">
        <property type="entry name" value="6-phosphogluconolactonase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005900">
        <property type="entry name" value="6-phosphogluconolactonase_DevB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039104">
        <property type="entry name" value="6PGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01198">
        <property type="entry name" value="pgl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054:SF0">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="8">
        <location>
            <begin position="102"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="54"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="63"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="326" mass="35334" checksum="9BD22C24C35D1002" modified="2017-01-18" version="1">MTTTVPKIYSFSEFAGVADAVANHVINAQNQALANSLPSNNSTTSVQNLQTLTSHSIPRQDPSTESSNSNSNLNTTATTTPSASSTNLASSGSGVTKTSKKAKEKAINKRFKIAISGGSLISVLAQGLLKRDDVVWDKWDIYFADERLVPFNDSNSNYGLAKMEIFDKLKELGKPQPKVYHIDESLLDDAQAVADAYEKVLIKGFANKDSVRLPLFDLFLLGCAPDGHIASIFPNDHEVLREKFAWVVPVENAPSGPSRRITLTIPVICHSHRVSFVVEGAVKASIVKVIMERPEKGLPASIVNEGSAGRIAWFVDKDALAETYFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4X6</accession>
    <name>A0A1E3P4X6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_92021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039159.1">
        <property type="nucleotide sequence ID" value="XM_019186333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4X6"/>
    <dbReference type="STRING" id="683960.A0A1E3P4X6"/>
    <dbReference type="GeneID" id="30203579"/>
    <dbReference type="OrthoDB" id="3980503at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019436">
        <property type="entry name" value="Say1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF31">
        <property type="entry name" value="STERYL ACETYL HYDROLASE MUG81-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10340">
        <property type="entry name" value="Say1_Mug180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="340" mass="37634" checksum="8411CEB2006B2B8B" modified="2017-01-18" version="1" fragment="single">LLAIVNLGGSVPIVPFPSITVGGDIIKYTEALVAAFVSLAKSKLPAAKILGLLPTLSLDFNLKVVEPFAKDLPNYNTEFPVKGLDEEKVKWLVEADNRTKEDPVLLYSHFGAYILGVLPVSPYLWTRLYQALENDRLSILLLDYTTTANGAKYPTQLKEIASTYNQLAKTSDNIIVGGDSAGAHLSLSLIRHSELPISGVPDVDGESLTGGIFISPWLNIYPDDTTGSHEYNNGKDFMISKFLREAGLEFVSSKDLLTAPETNFYNDTEADWENILPKNVFSSYGEREILLTDDVSWYKLAGLSGDQVFEDPNAIHDAIIFTPEKSDVFPKIVEFFKRNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P532</accession>
    <name>A0A1E3P532_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_29594</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039209.1">
        <property type="nucleotide sequence ID" value="XM_019181630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P532"/>
    <dbReference type="STRING" id="683960.A0A1E3P532"/>
    <dbReference type="GeneID" id="30198876"/>
    <dbReference type="OrthoDB" id="19329at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007117">
        <property type="term" value="P:budding cell bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041661">
        <property type="entry name" value="ZN622/Rei1/Reh1_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040025">
        <property type="entry name" value="Znf622/Rei1/Reh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13182:SF21">
        <property type="entry name" value="CYTOPLASMIC 60S SUBUNIT BIOGENESIS FACTOR REI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13182">
        <property type="entry name" value="ZINC FINGER PROTEIN 622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12756">
        <property type="entry name" value="zf-C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="4"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="393" mass="46353" checksum="9FCD3946A969C05F" modified="2017-01-18" version="1">MYTCNSCSLQFRTPEDQRSHMKSDWHRYNLKRRVANLPAIDEELFNEKVQKLSLEEENANETKGKKVSKKDQRRKEKEALLEKKKQLLEIAKQNMLKSMNSSNTGDKVEESKEQQEEIKEQVEQVQEDIKQENDEELTEEQQAEKIMEEKLKNKVEIPLEVCLFCNKKFPYFEECLNHMFKKHGFYIPEQKYLVDKEGLVKYMAEKVGLGNMCFVCSFQGRSLEAARAHMISKNHCKIPYESEDEKLEISEFYDFTSTYDDYKQVEGDENEDEWEDVEGEEGEEGSDDEEEELPQEVSYNDGIELHLPSGVKIGHRSLARYYRQNLKPETVLTEGQGTVIAAESRHLATVHDRAQLLEKKRVWKNEIHHKDRDDRRAAKFINNQPHYRDQLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P542</accession>
    <name>A0A1E3P542_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Adenosylhomocysteinase</fullName>
            <ecNumber evidence="7 11">3.13.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_62533</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 11">
            <text>S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine</text>
            <dbReference type="Rhea" id="RHEA:21708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.13.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="10 11">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <dbReference type="EC" id="3.13.2.1" evidence="7 11"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039164.1">
        <property type="nucleotide sequence ID" value="XM_019183391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P542"/>
    <dbReference type="STRING" id="683960.A0A1E3P542"/>
    <dbReference type="GeneID" id="30200637"/>
    <dbReference type="OrthoDB" id="10007170at2759"/>
    <dbReference type="UniPathway" id="UPA00314">
        <property type="reaction ID" value="UER00076"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000004">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000004">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00563">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004005">
        <property type="entry name" value="PRK05476.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF0">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10 11" id="KW-0520">NAD</keyword>
    <keyword evidence="4 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-adenosyl-L-homocysteine hydrolase NAD binding" evidence="13">
        <location>
            <begin position="194"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="160"/>
            <end position="162"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="225"/>
            <end position="230"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="302"/>
            <end position="304"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ59957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="450" mass="49032" checksum="A3F1DA557D6EE479" modified="2017-01-18" version="1">MSAPAVNYKVADISLAAFGRKEIELSENEMPGLMEIRRQYGAEQPLKGARIAGCLHMTIQTAVLIETLVALGAEVTWSSCNIFSTQDHAAAAIAAAGVPVFAWKGETEEEYLWCIEQQLFSFKDGKKLNLILDDGGDLTSLVHEKYPEMLEDCFGLSEETTTGVHHLYHMVRDATLKVPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVMLAGKVAVVAGFGDVGKGCAMALRGMGARVIVSEIDPINALQAAVEGYQVAPLDEVASIGQVFVTTTGCRDIITQTHFEQMPEDAIVSNIGHFDIEIDVAWLKANAVDVVNIKPQVDRYLLKNGRHIILLAEGRLVNLGCATGHSSFVMSCSFSNQVLAQIALFKANDEAFRSKHVEFGKSGPFEKGTVAVLPKVLDEAVARFHLKHLGVKLTTLTTKQSEYLGIPAEGPFKADHYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P585</accession>
    <name>A0A1E3P585_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_82994</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039836.1">
        <property type="nucleotide sequence ID" value="XM_019185470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P585"/>
    <dbReference type="STRING" id="683960.A0A1E3P585"/>
    <dbReference type="GeneID" id="30202716"/>
    <dbReference type="OrthoDB" id="5419315at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF32">
        <property type="entry name" value="ARGININE METABOLISM REGULATION PROTEIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="16"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="430"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="453"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="654" mass="75648" checksum="E26FD54749C0A89B" modified="2017-01-18" version="1">MPPKKRSKVYQRTFIGCWTCRRRKIKCDATRPHCLRCAKSKLECEGYDVKLNWSTPLVVKNGEMIFNDDENELDEPPVTFQRSNIALCKWNIYNYYEDIDDDLENLESLENNAKIGPFTVFRYKSISQSSSPILIQSIPQSRSTSFIPTTSPIRSKTPTRIPTRQQQHHLIHQSLIKFAKISIIAKGLDESTPLLFPLPSIASTSINIDPNRVLQPLIKNIDNSIMITSYFKLSINHFMTLIPQIFHNKKPDFINYIQSTIQTSLGQFILHNNDQDLQLLLNVTTVSLLHKLDLNNLSSLTEITQLFKMTSHLPITTNSDLTTLSLILQIMIATTLGIYNPLFFKQSPILQSPISSIYKYLKLSNILLNPSQFEMDPKYSKIYEDLNQSYNLLKNIKTKPKQKDLKNGYGMILIQSKETPRDQIIKVPKYIDDDDEGPPPPSFVVNFSHAEDESSEDDDESSEESLDDDHHQQSMTLFNLDDVPDLLKESSFGVSNCLIFIFEELVKLINHKRIFNFLKITSRNFPKVCADFEDILHKSNDLIIDSKCHNDLKFYNCLVLLYYKLVMNYPSHLLNYHFNALKNINPDVDLGLIWFVKHALDGHSSKNVELGQFDKVWLNNWESKQLIYELKLKNNNGDWANLMNLEKFNDFFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5G5</accession>
    <name>A0A1E3P5G5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_61252</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039892.1">
        <property type="nucleotide sequence ID" value="XM_019183143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5G5"/>
    <dbReference type="GeneID" id="30200389"/>
    <dbReference type="OrthoDB" id="3980104at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="760" mass="89073" checksum="453FECDB46398F9B" modified="2017-01-18" version="1">MVDRKLVAISLDPEDEDEEIVFCTYNLEGQFQKIRDQDSKFNETDFKSALKKFFQLDLPYIVYHDANPFELLDDECEVLNTRIFKPNDGVLERDTKIGNEIGYKFKKERVERVHDHSYLPGKPDMIYESYPFFKPDIDESYEFANEEFVLKFISKFFKSLFQSKNEIKATESLQYIRDLITFQNPVEYQNYFTIHNGEFYDVFQERIFQTILQIYEKFIFKNSAKLRKFFNDTDSSYHPILNSMDNISLKSIGSVATSPFAFGISDEDTKFLNALLVHVELVNMNLGIGEGYELYFNSKKQNFANQELIGTNSTKIDNFLKYFTKCVSICLNSKTNRFIITDYYWISVFEIEDVKDGEIEDVKRLISKVKVRHFSFSNFEKSGNIDENLTIRSIIASFFNVSPTSYKKNLKEMETLNKKVIKDWELFKKTESHSHVVTKSNNRKRLKFSPFTYSTPTGIQLQKQKVDLEKSLIKFIAIGYEWSCQTLSVDLKKLFQDKLHYSQLNFDKAGANKKGKEVLLSIYDAQYDSDHFEFDEYYRSIESKKSKASFDSILGLVKPDQSYFRWLSNVEVYEKLVGLQGDVIAKIIDYGYLEDKAYNDDELHFKTDGFYMIYEPISPNFQDMTPVDFKNEEHYQLAKETLSKIHEYGVSFHPRTKLTLEILRYYKGKVYVINLEEASSVPTKSSKNKDLIDLDRIFNKSTGNLEGGKERKRSRQHAHGHSHQHIFDFLAACEEDDYRPDYGSWSVSESEEGDEDGVMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5K4</accession>
    <name>A0A1E3P5K4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_14992</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039419.1">
        <property type="nucleotide sequence ID" value="XM_019180623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5K4"/>
    <dbReference type="STRING" id="683960.A0A1E3P5K4"/>
    <dbReference type="GeneID" id="30197869"/>
    <dbReference type="OrthoDB" id="2262349at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009074">
        <property type="term" value="P:aromatic amino acid family catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052780">
        <property type="entry name" value="AAA_Catabolism_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31644">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR ARO80-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31644:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR ARO80-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="7"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="45"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="127"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="730" mass="82575" checksum="39C7126568DB5F81" modified="2017-01-18" version="1" fragment="single">FRRNYKACLNCRTRKVRCDLGPVEAPHDPPCARCRRERKECVFADSRRGGSHGGGASSAGNSIGSSVNKKRKTESTPTPEVRSIPHTRQNSTEVQVTTLQGALVFLARAAGTIAKADDRDNISAREIHEKLEDQEDGISSTSNLSTRENSNTNILNQTQGSNDYENFAPLVRPQGKGPIMMPPVSKSANVHPNPSKKLSDIEYIGPNKMLTEEEAENLINLFFLTMHPFFPHIPTQLHDPQTLAQYPILLCAILTISSRYHPFENQPSHDINKNSRNIQLHERLWIYCQRLISQTVWAEASTRSIGTVLAFLLFTEWNPRAIHWRWSDYANNKDDMGPEGPPGLGGNSDEEDSLAGLGAMRRSDRMAWMLVGTSVRLAQDMGFINSSSKIFIATHIAETQTAMNLGQRSMLSQSLGEIDLDNDEDDDEVVDSKKQYQHDEFILSLDEECLRNRHFKKNNLKFSIMQKAKLELLKITSLAYETIYCGKYQVDGYNQFQNLSVLSILSPLIENWYKTYKKLLKPSNPKNFNHHCNLKNATNTKHVNEITKQVDKESLICDYYYCQLYIYSLALSAENPLNTNPSDVKKPLNNLKLDEISKAAKYVELAYNAAKEILVAAIRVHKLKMLKYMPVRWVTRIVRSVAFIVKCFLTLTNGAQQQLSSPLQQASQASTILTLSIIPTEEIIQTIQKAAIVLREASPDELHLCTRYSTILMYLCSEMKYRSKLSINPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5R3</accession>
    <name>A0A1E3P5R3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Altered inheritance of mitochondria protein 21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_77264</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in mitochondrial migration along actin filaments.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Actin patch</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIM21 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039791.1">
        <property type="nucleotide sequence ID" value="XM_019184526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5R3"/>
    <dbReference type="STRING" id="683960.A0A1E3P5R3"/>
    <dbReference type="GeneID" id="30201772"/>
    <dbReference type="OrthoDB" id="3981315at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021582">
        <property type="entry name" value="Aim21"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11489">
        <property type="entry name" value="Aim21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="650"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="226"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="269"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="331"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="390"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="499"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="517"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="541"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="565"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="584"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="652"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="687"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="714"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="760"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="789" mass="87969" checksum="1E12CA84F9B62F03" modified="2017-01-18" version="1">MSNLADIISQKREDSDPETNSFDEADDGFESETPQQHHVEEAPRPPISNPSSSSSIPKIPQRPVKKQTSESSSTPTPVIPKRPTRSQSPIVPPHPKRSESPIIPPRPKKSSESSKKEQSSDETEKTVPDLLKSTIEKIPGIGNLTKDENNEDDNTLKNDKESVPEPSKGEIKPEEIVIPPRPKKSSSSEEETAKPIEESKSTNTPEIPARPTHRPKSKSVKAEEEPKNEEDAQFPEEEKKTPLASTSEESTKADLIVEDNSRDIPVISNDKEKVEEPITEAKQEDVSESPKDEIVEVSKPVQDIKTESEAFDVSPVEIEDKPGEIITEADVVEHSHDTVKDGSEAPKSIETEADAIEKLEEQPKVEEAKEPKEESPLPKVPQRPSKRPTKITTPSVQEASVTSEDKADNVEESTDDIISKTNEELQNIQAHFSEKKSSTSSIPPRPIKKASTSSSNEIFGLYTSDSQDDSTKPKPKSTKAPPPPVPKKPSSKIAAFQEMLKRNQEQERQQQSQSFQRSERSDSNDSTDRSKFKQNLNGLFALPGMARPGAPMPGMFKQEESESKNDEEETEDEPKEVKQDVRRSRAKGPRGKRTAKVEKIEIPDHRVIKVIDIWSSGLESDAKVTYENTKESETKSDVITDDIDDIELINPVEEKDKVDESLKPNDEDLKGYEKGLKDDTETEVEELETKSTITKEEETPAPSEVKKEDKGYESDNEDVIKDEEDDEEDNVELNPSIQSIKSKGDSIPDASIRSIKSETLELDDDGENDETYEKVETPEAIDEFVDAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5T6</accession>
    <name>A0A1E3P5T6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Palmitoyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_14664</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="11"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039847.1">
        <property type="nucleotide sequence ID" value="XM_019180616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5T6"/>
    <dbReference type="STRING" id="683960.A0A1E3P5T6"/>
    <dbReference type="GeneID" id="30197862"/>
    <dbReference type="OrthoDB" id="9909019at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031211">
        <property type="term" value="C:endoplasmic reticulum palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF43">
        <property type="entry name" value="PALMITOYLTRANSFERASE APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="12">
        <location>
            <begin position="135"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ60640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="317" mass="37023" checksum="442D4867805F63A5" modified="2017-01-18" version="1" fragment="single">LLNWLITDPITPKDESLPVARNYQGWNYGNLVFLAGGRFRYVKSRPISFATGFLVVLPGILFCIFDASWCWHNISPAVVITFLYTWLICISSFIKAATSDPGIIPRNIHITDEPYNLPDEYFNTITLPSYKEKSIEVKYCLTCRDWRMPRTFHCSRCDQCISIHDHHCIWINNCVGERNYRYFFAFLLFGCICSVYLSVLAYYHIFIYQKTHSLSFKQTLDHVPMSFFNAIYGNLILLYPLLLLLYHMFLVSTQQTTREYLRSLKTKNNRNPYSLGNIVQNIITSLCKPRGYSFISTRASFEPGDRRFVRYPAATQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5U5</accession>
    <name>A0A1E3P5U5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CCA tRNA nucleotidyltransferase, mitochondrial</fullName>
            <ecNumber evidence="7">2.7.7.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">CCA-adding enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA CCA-pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA adenylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">tRNA nucleotidyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_28504</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Nucleotidyltransferase that catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs, which is necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded. The structural flexibility of RNA controls the choice between CCA versus CCACCA addition: following the first CCA addition cycle, nucleotide-binding to the active site triggers a clockwise screw motion, producing torque on the RNA. This ejects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a second CCA catalytic cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14433"/>
            <dbReference type="Rhea" id="RHEA-COMP:10465"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="EC" id="2.7.7.72"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72" evidence="7"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040023.1">
        <property type="nucleotide sequence ID" value="XM_019181493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5U5"/>
    <dbReference type="STRING" id="683960.A0A1E3P5U5"/>
    <dbReference type="GeneID" id="30198739"/>
    <dbReference type="OrthoDB" id="445712at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052929">
        <property type="term" value="F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052927">
        <property type="term" value="F:CC tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:CCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000019">
        <property type="entry name" value="tRNA nucleotidyltransferase cca2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3090.10">
        <property type="entry name" value="cca-adding enzyme, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13734:SF5">
        <property type="entry name" value="CCA TRNA NUCLEOTIDYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13734">
        <property type="entry name" value="TRNA-NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81891">
        <property type="entry name" value="Poly A polymerase C-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly A polymerase head" evidence="14">
        <location>
            <begin position="45"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA nucleotidyltransferase/poly(A) polymerase RNA and SrmB- binding" evidence="15">
        <location>
            <begin position="214"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00050431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF12627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ60816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="510" mass="58563" checksum="E2CCBCA89BF7222E" modified="2017-01-18" version="1">MVFTSAFDVKNPTVQLNETEQKISQLLKDFSNDYNSQNDGEKLELRITGGWVRDKLLGDESNDLDIAINKLTGEEFGENLRQFLIDNFDTYQISPKNVHKIDKNPAKSKHLETATTKLYGLDVDFVNLRSEEYTEDSRIPIIKYGTPQEDALRRDATLNALFYNIQKDQVEDLTERGLEDLRDGVLRTPLKPLKTFLDDPLRVLRLIRFASRFGFSIEEETFNAMKEESIKAALIHKISRERVGVEIEKTLQGKNPVYGLELINELKLMRSIFNFGPFEDVICGGNDKSQVDSIFGEVEKQASNSIAVVKNFQSETKLDSVFNNVIQDKELKMLFWLSIILRPWRNTKVHVKKPIFATEIIIKEGVKLSKLDADVVTKLVSTSEEYQSVVNQLPGFKRSTIGVLLKQYGENLNLSIMFNFFDELIKTNDTESTISKYESFYHVILKENLQDSYKLKPIVDGKTLIKKLNTKPGPWMSKITEEIMVWQLDNPEKSQEDCISFVASILEQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5X8</accession>
    <name>A0A1E3P5X8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_62809</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039454.1">
        <property type="nucleotide sequence ID" value="XM_019183442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5X8"/>
    <dbReference type="STRING" id="683960.A0A1E3P5X8"/>
    <dbReference type="GeneID" id="30200688"/>
    <dbReference type="OrthoDB" id="10530688at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133718" evidence="2">
        <location>
            <begin position="19"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="193"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="250"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="315" mass="31580" checksum="638570F2E6D4F8F8" modified="2017-01-18" version="1">MKFQILASIVAGLSLVSASPVAEALPWAQANPQAAAAANAYADAYAEAVALAHPDPEAYALAASADDCASISCHASCGLLIIEAQSCSQNKENSYSGPYNETCLCSSDSKFIQYYSPCMDCGWTLWKYYGVYVTSALEACKTLSTEPTGTSRCSTTLEGTYTPDYNIQGCSYLGNCESTTSAAAAESSAEQSTSTSAAASSTAAEQSTTSAAAQETSSTAQQASSTGAAQTTSAAETTSEQLSQSAAPTSEAQGQSSAAAAGSSEAQGSAVVAQSSSASAGAGASSTPVVQTFEAAAVKVGATSAVFAILFSLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6U9</accession>
    <name>A0A1E3P6U9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_28423</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040227.1">
        <property type="nucleotide sequence ID" value="XM_019181484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6U9"/>
    <dbReference type="STRING" id="683960.A0A1E3P6U9"/>
    <dbReference type="GeneID" id="30198730"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF81">
        <property type="entry name" value="PROTEIN ABHD13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="88"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="294" mass="33256" checksum="79BD2B322AFFEC22" modified="2017-01-18" version="1">MSSLLKMFLATTASVTTASLLLLYKYQLKLIYPSGLNDARAPGAFHSTPQEYEIPYEDLYLETEDGVKLHAFLCLQDPKSPDYQNKTVLILNPNAGNIGLAVPTVYNFYQLGFNVFIYDYRGYGLSTGESTEIGLKKDTETVMEYINEHDQLNKSLVILYGRSLGGAVAIHIASKNYELVKGVILENTFLSLRKNIPHVFPFLKYVATLCHQLWDSETAISKIDPNVKMLFLGAELDEIVSPDHMKKLYELANVGDRYEKFVLFQGFHHNDTTLAPGYWDIISDFIDLIDSKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7W8</accession>
    <name>A0A1E3P7W8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_61575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040219.1">
        <property type="nucleotide sequence ID" value="XM_019183202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7W8"/>
    <dbReference type="GeneID" id="30200448"/>
    <dbReference type="OrthoDB" id="4026150at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="313" mass="36741" checksum="F03BC656428B06F5" modified="2017-01-18" version="1">MSKLVSRWADDPELVEEAKKQDHSKSSPNKSQIEDSKWASPIPSQKGKALESKWASPVTSEKGKPLVSKWADAPEEEEISPTKNQNKHPKKHTPRKHHNKQEDHVDLSQKFEKNLQLEEEEEDDRVEMSPEARKFASRLGISTDNERSSVASKPKIQFKKEPRYQPESEGDDWEDEEEEELEAKPVPPTKAAKDFASRLGVTIDDKKAIRNKREEERNRLKNERINACREWDHGKNSHTKPDQRSKRESPRKHNRFTKPRAEQTQSKPEPEYYEPEPMTEAKRKEQQEHMERLDYFIANHQDLDWADFDDDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7W9</accession>
    <name>A0A1E3P7W9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_83586</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040665.1">
        <property type="nucleotide sequence ID" value="XM_019185566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7W9"/>
    <dbReference type="STRING" id="683960.A0A1E3P7W9"/>
    <dbReference type="GeneID" id="30202812"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032126">
        <property type="term" value="C:eisosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000006">
        <property type="entry name" value="General amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004762">
        <property type="entry name" value="Amino_acid_permease_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00913">
        <property type="entry name" value="2A0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF19">
        <property type="entry name" value="LYSINE-SPECIFIC PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="442"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="475"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="40"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ61458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="537" mass="59690" checksum="BB1C2FC4DC7821D0" modified="2017-01-18" version="1">MKSENYLEKSIPSKESTLEIQEKHGHVKETQVKRALKPRHVSMIALGGTIGTGLFVGIGTPLSLAGPVNALIAYLFMGSIVYLVTQSLGEMCTFIPVTSAFTVFNARFLSPALGVANGYMYWFNWATTFAVEISVVGQVIEYWTDAVPLGAWIAICWFIMTVANFFPVKFYGEIEFWVSFVKVVAIVGFILYSFIMVCGAGDQGPVGFRYWRNPGPWGPGHLVESVPTGRFLGWVSSLINAAFTYQGTELVAISAGESKNPRKTVPRAINKVFFRILFFYILSLFFVGLLVPFNEPRLNSTKAVISSSPFIIAIENSGTPILNHIFNAVIVITVMSAANSNVYVGSRILYGLGISGNAPKQFTYVTKQGVPYLGVICTSLMGFLAFMVLSDSAAVGFNWLVNITTVAGLFAWLFISICHIRFMQCLKSRGISRDELPFKAHFMPWGAYYAAFFVFIIIFIQGYAAFFSFTATKFFTAYVSVIIMAVLWIGCQIFYRGPLFLSLDDIDIDTDRREIDAIVWEDDAPKNIWEKFWAAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7X4</accession>
    <name>A0A1E3P7X4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_27879</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040728.1">
        <property type="nucleotide sequence ID" value="XM_019181422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7X4"/>
    <dbReference type="STRING" id="683960.A0A1E3P7X4"/>
    <dbReference type="GeneID" id="30198668"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF13">
        <property type="entry name" value="SUBTILASE-TYPE PROTEINASE RRT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="6">
        <location>
            <begin position="72"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="333" mass="35394" checksum="9BE527AEDCB78216" modified="2017-01-18" version="1">MGLFKGIMGEFNADMINSLYYDKNIASISIDRELVLAEVQENAPKHLARLSQKEALKNNQKFDFVYDPSGGIGVDVYILDSGIHALHSDFSRRVTKIADFTGEGGGQEDSVGHGSYMAGVIGSETYGVAKKSNLFDVKVTDKKGRTKLSAVIGALSLILADSKVTNRPTLVVVPLIMKKNAILNGAIESLVKEGIPVIVPAGNDGKQSCNYSPASAKGALTVGSIDVETDSIASFSNWGACVDVFAPGTNIKTTSNHKNEVVTKSGTSLSTGITAGLVAYFMGMGDSGKEAVQRVIQYSNADSIPEESLNFKPLTANRIVFNCEGNPNWGPSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7Y6</accession>
    <name>A0A1E3P7Y6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">assimilatory sulfite reductase (NADPH)</fullName>
            <ecNumber evidence="5">1.8.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_27211</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>hydrogen sulfide + 3 NADP(+) + 3 H2O = sulfite + 3 NADPH + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>siroheme</name>
            <dbReference type="ChEBI" id="CHEBI:60052"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.2" evidence="5"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040746.1">
        <property type="nucleotide sequence ID" value="XM_019181346.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P7Y6"/>
    <dbReference type="GeneID" id="30198592"/>
    <dbReference type="OrthoDB" id="1688044at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009337">
        <property type="term" value="C:sulfite reductase complex (NADPH)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050311">
        <property type="term" value="F:sulfite reductase (ferredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004783">
        <property type="term" value="F:sulfite reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000097">
        <property type="term" value="P:sulfur amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.413.10:FF:000003">
        <property type="entry name" value="Sulfite reductase [NADPH] hemoprotein beta-component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.413.10:FF:000004">
        <property type="entry name" value="Sulfite reductase [NADPH] hemoprotein beta-component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000016">
        <property type="entry name" value="Sulfite reductase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="entry name" value="Sulfite Reductase Hemoprotein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045169">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006066">
        <property type="entry name" value="NO2/SO3_Rdtase_FeS/sirohaem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010029">
        <property type="entry name" value="PRK13504.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493:SF47">
        <property type="entry name" value="SULFITE REDUCTASE [NADPH] SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493">
        <property type="entry name" value="SULFITE REDUCTASE [NADPH] SUBUNIT BETA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00397">
        <property type="entry name" value="SIROHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="Nitrite and sulphite reductase 4Fe-4S domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="Nitrite/Sulfite reductase N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00365">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="14">
        <location>
            <begin position="663"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ61539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1423" mass="159810" checksum="CBB906B2B0D94EB8" modified="2017-01-18" version="1">MVLEDFYSYVSANAGAGEFVVYSTLESKSQKTIIPSDHVLLNFSDPFQSIIESQESQESASIIFTNDQTVLKALPHLQSLRNNTIINIALSNYQSVIALKDLNFTTLISSTKEDVLNALTLSHILVNSTNSPILHFINESLVEQSPNVKISKSLSKILETQATDLVDGLNILNSNGMKYNYVDFSKGSRNSKVALVNLGSHYNDLKSSGLPTINIKIFKPFEISQILGNLTSSVEAIAILEDSNSKPLTKFSPLLLEFFQDPTYLVDNGILKITSSSIGDYDDDFNKLAQIVTSNLSLDYPIQNLYFGNESQIELSDIKVNEIEKISKLESSYLTILDQVFGSNLKIYNQFNSENINASSPEFGFGSYIYLNEQKQKLIKLIHASLDTPDEKFQTPKLNELVEKLSKWLVYHRELEGLSQDKINTYNQLSIEIIKLLKEDDSETSKNLLSLENSFFINSNWLIGSDSWAYDLGTSGIHHVLESNENINVLVIDNEPYSTKTTKTKSLRKKDIGLYAMNYGNAYVGSIAIYSSYTQMLTTLIEAAQFQGPSIILAYLPLKNEFDTPLDILTNTKNSVESGFIPLYRYNPFLDSKDSFKLDSTVIKEQLKNFLDRENKLTLLSAKNPQFARSLKQSSTSIIKQKQDKKAQQAYDQLLEGLSGPPISIYFASDGGNAENVAKKLGKRASARGFKSTVLSMDDIILEDLVTEELILFITSTSGQGEFPQNGKNFWDSIKNSGDLDLEFSKISVFGLGDSEYWPRAQDKHYYNKPAKDLHSRLLFLNAKELIPLGLGDDQDADGFQTGYKEWESQLWKALGVDDIKTADEPPPITNEDIKIASDFLRGTIADGLKDESTGAIASSDQQLTKFHGIYMQDDRDLRDSRKAAGLEPFYIFMARVRLPGGITTPDQWLMLDRLADERGNGTVKLTTRATYQLHGIVKHNLKPSIRAMNSVLMDTLGACGDVNRNVMVSALPSNAYIHGEVNNVAKRISEHLLPQTTAYHEIWLKGDDPEDDEDANWPETFEQRKDGPKKIKTLVAGNALVDTEPFYGPTYLPRKYKINITVPPYNDVDVWSSDVGLITIIEDNEVVGFNVLVGGGMGSTHNNKKTYPRTGSSFGFVPTDQAHLVCEKIMLVQRDHGNRKDRKNARLKYTVDTLGVEVYKEKVEELWGQKFQPERAYEIKDNIDHFGWVTDELDQHHFTCFIENGRVEDTPELPQKTGLKKIAEYFKSNPKSKGTFRLTGNQHVLISTVSNEELPTVKQLLHDYKLDNTDFSALRLSSAACVAFPTCGLAMAESERYLPVLINKIEEALEEYGLRHDSVVMRMTGCPNGCARPWLAEVALVGKAYGVYNLMLGGGYFGQRLNKIYKQSIKEDEVLSILKPLFKRWALERQDGEHFGDFLVRVGVIKPTLEGKYFHDDIPEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P866</accession>
    <name>A0A1E3P866_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glucosidase II subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_27703</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism; N-glycan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040274.1">
        <property type="nucleotide sequence ID" value="XM_019181401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P866"/>
    <dbReference type="STRING" id="683960.A0A1E3P866"/>
    <dbReference type="GeneID" id="30198647"/>
    <dbReference type="OrthoDB" id="1334205at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017177">
        <property type="term" value="C:glucosidase II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090599">
        <property type="term" value="F:alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06603">
        <property type="entry name" value="GH31_GANC_GANAB_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033403">
        <property type="entry name" value="DUF5110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF54">
        <property type="entry name" value="BCDNA.GH04962"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17137">
        <property type="entry name" value="DUF5110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133747" description="Glucosidase II subunit alpha" evidence="11">
        <location>
            <begin position="20"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 N-terminal" evidence="13">
        <location>
            <begin position="87"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="12">
        <location>
            <begin position="367"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="15">
        <location>
            <begin position="700"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5110" evidence="14">
        <location>
            <begin position="805"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ61067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="913" mass="104722" checksum="31F4B29772367EC5" modified="2017-01-18" version="1">MKLFQFFILLTIYLQGAFAVKDYLFKKCAQSGFCNRNRHFAGEVSKNPDYVSKYSIDESTLNYIESNSTILGDIYKKVNDAKSVRLPFQINILKNNNIRLRVDEDRSQIEWNNKFLNKERFADAWKYAFRGEPVVSQNYKFNDLQNGKISVIYGDKDQYELEIFTQDFKVVVKYNGETQLVLNDRSFLNIEHFRKEDENEANLTPEESSFNSFHDDFQDSKDDSVPFGPESVALDVTFKGFKHVYGIPEHADSLSLKDTSESEPYRLYNVDIFEYETDSRLPMYGVIPFMVAHKVGVSAGIFWVNGADTYVDVKKDQESQDEQVLSSKTDSKTHWISENGILDVVIIIGETPTEVTQSYASLSGNVQLPQLFSLGYHQCRWNYNDEEDVLTITENMDKFEIPYDVIWLDVEYTDAKKYFTWKKELFPDPERMLNKLDETGRTLVAIIDPHLKTGYEVSKDVESKDLALLNKDGDIFKGHCWPGESVWIDTFNPKSYDYWTYLFRNSTGLAGDATNFHIWNDMNEPSIFSGPETSSPKDLVNYGGLEIRSDHNIYGLTFHEATYQALIDRYVNKRPFILTRAYYSGSQRSAAMWTGDNMSKWEYLKISLPMILTSNIAGMPFAGADVGGFFGNPSKELLTRWYQAAVFYPFFRAHAHIYSRRREPWIAGEPYTSYMRDAVKIRYSILPLIYTKFYESSKTGAPVINPLFYETPSNPKTFEIEDEFFLGGLLVKPVTDEGAESVEIYLSDEEIYYDYETFESLQGEGDHKVDAPLGKIPIFIKGGSITPRKDRYRRSSKLMKYDPYTLVIALDTNKSAKGSLYIDDGETFNYENGEFLYIDFSLQDGELQAAVKDGSLSVPNSIEKILIVGLESAPASVQVSQGSEWNADVIQKTNYAIIKTPGVDLSKDWSIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8C7</accession>
    <name>A0A1E3P8C7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein L34e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_37783</name>
        <name evidence="4" type="ORF">WICANDRAFT_61764</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
            <strain evidence="4">NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL34 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040409.1">
        <property type="nucleotide sequence ID" value="XM_019183242.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041108.1">
        <property type="nucleotide sequence ID" value="XM_019182528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8C7"/>
    <dbReference type="STRING" id="683960.A0A1E3P8C7"/>
    <dbReference type="GeneID" id="30199774"/>
    <dbReference type="GeneID" id="30200488"/>
    <dbReference type="OrthoDB" id="277449at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.370.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008195">
        <property type="entry name" value="Ribosomal_eL34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038562">
        <property type="entry name" value="Ribosomal_eL34_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018065">
        <property type="entry name" value="Ribosomal_eL34_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10759">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01199">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01250">
        <property type="entry name" value="RIBOSOMALL34"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01145">
        <property type="entry name" value="RIBOSOMAL_L34E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="13953" checksum="9D813CED9671BA37" modified="2017-01-18" version="1">MAQRVTYRRRNPYNTRSNKIKVVKTPGGVLRAQHVKKSGTRPKCGDCGIALAGISALRPREYAQVSKTHKTVSRAYGGSRCANCVKERVVRAFLIEEQKIVKRVLKEQAEKEQKAEKKASKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8K3</accession>
    <name>A0A1E3P8K3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_25282</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040953.1">
        <property type="nucleotide sequence ID" value="XM_019181124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8K3"/>
    <dbReference type="STRING" id="683960.A0A1E3P8K3"/>
    <dbReference type="GeneID" id="30198370"/>
    <dbReference type="OrthoDB" id="10263353at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF16">
        <property type="entry name" value="U4_U6.U5 TRI-SNRNP-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="6">
        <location>
            <begin position="20"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="5">
        <location>
            <begin position="142"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="436" mass="51266" checksum="D419A441C793A514" modified="2017-01-18" version="1">MSRHSSDEEPTAKRAKLPTTDEIYLDTIDRKRLDFDFEKICSVTLSNINVYGCLVCGKYFQGRSHSSIAFNHSINEDHHVFINFNNLKIYVLPENYEVKSTMLNDIKYQVLPSYTEQDLKKFQDEGFKSVDLNGNDYIPGYIGLNNMKANDYSNVVIQALFHVPLIRDKLLLSSLDNFKNEMNKRVIILIKKLWSSRLFRNHISPHELLQYISILSNKKFNINEQKDPKDFLVWFLNSLGKSFKEDGEGLISKSFQGKIQIQSKKENDDQVKEVVTKFWLLNIDLPPIPVIKDNHEAKHIPQVKLTKLLDVYQKGEEVLLANGEVRKYKILKLPKYLILHFDRFKDKALNLDLSVKDRNQTMVEFHLEIEILGVKYKLISNIIHDVSESQDEDQSDWKVQLKHHSEWFEIENLIVKPKEKEFLFLNETYLQIWERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8L5</accession>
    <name>A0A1E3P8L5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dolichol phosphate-mannose biosynthesis regulatory protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_28825</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Regulatory subunit of the dolichol-phosphate mannose (DPM) synthase complex; essential for the ER localization.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the dolichol-phosphate mannose (DPM) synthase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DPM2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040499.1">
        <property type="nucleotide sequence ID" value="XM_019181541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8L5"/>
    <dbReference type="STRING" id="683960.A0A1E3P8L5"/>
    <dbReference type="GeneID" id="30198787"/>
    <dbReference type="OrthoDB" id="311279at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033185">
        <property type="term" value="C:dolichol-phosphate-mannose synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180047">
        <property type="term" value="P:dolichol phosphate mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009914">
        <property type="entry name" value="DPM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15039">
        <property type="entry name" value="DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15039:SF11">
        <property type="entry name" value="DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07297">
        <property type="entry name" value="DPM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="81" mass="9543" checksum="D9DFCB201D5FC38D" modified="2017-01-18" version="1">MLVWFTLLFVYYLSWLFLTPFLDQDSIIQNLYPPIDYAIKIPLFLLLIAFVTIGTYVSLLLINSNQPREHDALRPKGQKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8L8</accession>
    <name>A0A1E3P8L8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NIF system FeS cluster assembly NifU N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_20776</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; iron-sulfur cluster biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the NifU family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040962.1">
        <property type="nucleotide sequence ID" value="XM_019180885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8L8"/>
    <dbReference type="STRING" id="683960.A0A1E3P8L8"/>
    <dbReference type="GeneID" id="30198131"/>
    <dbReference type="OrthoDB" id="1925777at2759"/>
    <dbReference type="UniPathway" id="UPA00266"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1010.10:FF:000005">
        <property type="entry name" value="Iron-sulfur cluster assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011339">
        <property type="entry name" value="ISCU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01999">
        <property type="entry name" value="iscU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIF system FeS cluster assembly NifU N-terminal" evidence="10">
        <location>
            <begin position="2"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ61755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="126" mass="13759" checksum="65D7687AC71C43CD" modified="2017-01-18" version="1" fragment="single">RKYHPKVIDHYQNPRNVGSLDKTLTTVGTGLVGAPACGDIMRLQIEVDDKSGIIKDVKFKTFGCGSAIASSSYVTELLRGKTLEEAAKIRNNEIAKELSLPPVKLHCSMLAEDAVKAAIKDYKTKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8T3</accession>
    <name>A0A1E3P8T3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GYF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_76009</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041026.1">
        <property type="nucleotide sequence ID" value="XM_019184309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8T3"/>
    <dbReference type="STRING" id="683960.A0A1E3P8T3"/>
    <dbReference type="GeneID" id="30201555"/>
    <dbReference type="OrthoDB" id="48509at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051640">
        <property type="entry name" value="GRB10-interact_GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003169">
        <property type="entry name" value="GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445:SF36">
        <property type="entry name" value="FI03272P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445">
        <property type="entry name" value="GRB10 INTERACTING GYF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02213">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00444">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50829">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="2">
        <location>
            <begin position="225"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="641"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="795" mass="87123" checksum="FBA0A18A1BD99FA9" modified="2017-01-18" version="1">MSQSPSLNFGPAWMRPANSRTSSQDPNVSNLKGVKAWGLQDQTQQQQQQQQQQHLATSLNDPTFQQPSLQQPSSFVTQAQKNGNGSRVYSFEDLLKIWQEIQEKIKNGESVESYRSLSPIKSLVEQVERDRSFQSDLKKDDPQVSEITEKLDEITRWAKNEKPTPSSTTTAGGLASLLNKDTSTASPFLAQKSLLNDHPLHSPYGNTSADVPLAQNSLSLLQPHQIKWFYLDQTGNQQGPFDGNLMQNWYMSGYLAPDLNLRREDEYTYYSLQNFILSVNSYQQPFLVSLPDLTPKPQSVPFQHSLSFEQQLPGFFNQGIPQAQSQFGQVQNVQQNWSTNSGHSSPWVSQTNLASEVPIATQDFRIGTQSPFMNTSFNNQSTSSLARASVDTASNEHDELLDQIHSKVLNNVLEDEIEPQTVEPVVEPKVEESIKVPEPKETAPIAATQKAVGTPSNATQQQTKPASNGHVATASASETAKLLKPEAPVAQQTPKLAPWANKSVSNAPQLTLEQIQRLEAEESEKQAKAKAKQDKILAAQLLANTEKTIKETEASKKILPSTSSWGTPTKATPAPTKTLLDIQREEEAAAVAQAAQAAAALAASTSSSSGSTPMKKAPSAKGSFASIATSSAPPANAWTVVSSNRKPAPKPAPKITTAPKPITPEVLRNVSASTAAQVQPSSTYTKTISPRQDFLSWCRTSMKLNKGVNKDEVLQIMLMLPVGSESSEIIADTIYSNSSTMDGRRFAQEFMKRRKAVEDQAHDGLSWNEALRISAENDDDGWDFQVVGKKKKGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8V1</accession>
    <name>A0A1E3P8V1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Branchpoint-bridging protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_19002</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Necessary for the splicing of pre-mRNA. Has a role in the recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract and the 3'-splice site at the 3'-end of introns.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the BBP/SF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041005.1">
        <property type="nucleotide sequence ID" value="XM_019180812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8V1"/>
    <dbReference type="STRING" id="683960.A0A1E3P8V1"/>
    <dbReference type="GeneID" id="30198058"/>
    <dbReference type="OrthoDB" id="6777263at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045131">
        <property type="term" value="F:pre-mRNA branch point binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02395">
        <property type="entry name" value="KH-I_BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032570">
        <property type="entry name" value="SF1-HH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047086">
        <property type="entry name" value="SF1-HH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF45">
        <property type="entry name" value="SPLICING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16275">
        <property type="entry name" value="SF1-HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9 13" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="13" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="15">
        <location>
            <begin position="250"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="288"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="340"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ61798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="405" mass="43700" checksum="552B78BFEE3F3FDB" modified="2017-01-18" version="1" fragment="single">NLPTVINGILTPEQLDAYQIVYRIEEITQKISTNDIAPPKKLSRSPSPPPQYDASGKRTNTREARYKKSLEDERHHLIDIAARLVPHYVAPTDYRRPKSLTQKYFIPVKEHPELNFIGLLLGPRGNTLRKLQETSGAKIAIRGKGSVKEGKNRGITQQQNNLEDPLHCLITGDTEEKVLAGIEACKDVVNRAISTPEGQNDLKRGQLRELAALNGTLREDDDRPCTICGLRGHKRYECPNRTNYTSQVVCRACGNTGHFARDCKQRNTGGARGPFDVDQEFDNMLKDINVNNYTRNNEDEQPYKRRNINPQSPFPGKPPAAATSQPPGSFAPPGVSAPPGLTPPPGLAPPPGVSAPPGLAAPPGLSAPPGLSVSPSFPSIGPAPQGMTPPPGISAPPGIGAPPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8Z6</accession>
    <name>A0A1E3P8Z6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_59962</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041092.1">
        <property type="nucleotide sequence ID" value="XM_019182909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8Z6"/>
    <dbReference type="GeneID" id="30200155"/>
    <dbReference type="OrthoDB" id="5328412at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="500" mass="57899" checksum="E85F80B13C7118A9" modified="2017-01-18" version="1">MPPKKTPKIPKFPKKSSPSPSAPTLITANDYLDHGVEEEESGDRWIISDLSKSLRFYQRAYENYIEAINKDSTLVDAYYNANRLLYHVWEVYNTVPPSHLKNVDGCDVVKYSLREIKDKHEFVVNKFDEAGLTWEMYYNLILINTELIENEDVVGIDAVLKSCEDATVLMGKVLDYQLIELDSFLKRLENPEYEEEGKEDENEDNFDVVEQITPSVVLDSIITGAKFVQASYEFCDNYIQINALKNFFQSSGFEARLQQVLQLLLSKFTVENNAQDEFNLNFKNEEIESVKLVNHSISAFFINDFEQLVNHWKTLGNELDKSPSRFMAEADCFQSFKDRIDSITSEQQWVLINHINANLKKAQELVKEELTIHIQDKTNEVSSKVSKIIEILLSRADNELLRSSLQDLESSAKNRDVLRQNTVNLLKSGLNMGNTNCGLRETISDKLKREKLRRECVVRLVVLTKEQFTEQDLIRNLGMDHYMKEVDDLKNNELYRAYFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9B9</accession>
    <name>A0A1E3P9B9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_98331</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040759.1">
        <property type="nucleotide sequence ID" value="XM_019186649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9B9"/>
    <dbReference type="STRING" id="683960.A0A1E3P9B9"/>
    <dbReference type="GeneID" id="30203895"/>
    <dbReference type="OrthoDB" id="2962993at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000034">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000068">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF18">
        <property type="entry name" value="NICOTINIC ACID TRANSPORTER TNA1, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G03820)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="396"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="431"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="458"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="51"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="520" mass="58107" checksum="6DBF6E2FCB1AF260" modified="2017-01-18" version="1">MSFHSLDKPEAEIVNRSDSDSRNGVLTNNQEEDDWSDIDEKKVLRKIDRNLLPLISVLYLLAYLDRGNIGNAKIEGLVEDLGLSTNEYNLCLTIFFLTYSFFEVPSNMILKKIGAQSVYIPSIMLGWGIVMVCMGVVKDFKGLFVTRLFLGVFESGLFPGVAYCLTLYYCKGEMQYRQALFYSAASIAGAFSGLLAFAIAKMDGVGGYRGWRWIFILEGLLTVVVAVSAYFLMPDYPDTAKFLTPREREFVQWRLDHDNNFKKNQVSLENTEKVQRAEPNFEGFNDSNDVSLKTALFLVFKDWQILLHVLLYWGISVPTYGVSLFLPSVIKGLGYTSSIAQLLTIPIYVTASICSLIQAYFSDKAGIRSIFIGTNLFFVMIGFAMAIAGTDRDVPGVIYAGCFIATIPLYMAFPGIIAWNSNNLSNAKKRAVGMALHIGLGNLGGAFASNFYKPDNYIMGHGLVMGFATMGLLSCGLLVAGYKASNKKREERLIEGKYDEVSDEEIFRMGDKSPYFRYRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9K9</accession>
    <name>A0A1E3P9K9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_76278</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041306.1">
        <property type="nucleotide sequence ID" value="XM_019184355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9K9"/>
    <dbReference type="GeneID" id="30201601"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="125" mass="12611" checksum="8DBB149F0A41E876" modified="2017-01-18" version="1">MNLLSFSSSSINHQKSSNFSLSFITTTTITMQFQLVALLSIAALASAQNASTVIVDNESTITSSIEDCGPSVTNCPAKSTAHVNSTSSNGTVAPPTVSTFEAGAGRAIYAQAGVAALAGVAALLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9P6</accession>
    <name>A0A1E3P9P6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LAA1-like C-terminal TPR repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_75878</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HEATR5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040879.1">
        <property type="nucleotide sequence ID" value="XM_019184281.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P9P6"/>
    <dbReference type="GeneID" id="30201527"/>
    <dbReference type="OrthoDB" id="3980435at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040108">
        <property type="entry name" value="Laa1/Sip1/HEATR5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046837">
        <property type="entry name" value="Laa1/Sip1/HEATR5-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057981">
        <property type="entry name" value="TPR_LAA1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21663:SF0">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21663">
        <property type="entry name" value="HYPOTHETICAL HEAT DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20210">
        <property type="entry name" value="Laa1_Sip1_HTR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25808">
        <property type="entry name" value="TPR_LAA1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LAA1-like C-terminal TPR repeats" evidence="2">
        <location>
            <begin position="1740"/>
            <end position="1892"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1906" mass="216830" checksum="0B303F955310774E" modified="2017-01-18" version="1">MDNFKFEALVSSIETGQVARTLLSYHINLYKFIINKSSPIDSNSQTFILQQLESQIDYFQDDKNKEKLDLVSQPLRVAIAKNYVTILGLNKHKLFDTTNRFVTLFADISKKKSHYTALKNLSCVVLSFIYESFGHELLSFVALLNSTILRYLKKNQESKTIIADYSLNLSNLLSVILRNGGIAELDESITTKFLKLFKHTINDDSSSVQLIANLYESWGIISTHKKPEHSVYQLAHSSAILAGLGSKEKIIRVSCAKALAESLKLVSFKLKTVFSIYIELYIEGNLFAKVGITESIIQFLHLHVAEEYEYFLNHFEEVLSLLLSIFDHERVHSASRNCTSRIINQIDYIFETLLKLTGESGQRMIFDKLFKSYLNSEEKEHPFKIITVLKIVKKVLSNLSSLSEADLEYYQITMMKLCTSPNFEIRIHSVEIIKTFAAGFPHLINELLDTSFNELANNYKQASEKRLNFVKTHGLSLIIANLISLADKDYVPFELILNIWDLVTSKLRAKNQSLKDSVVYYTILTSWIVMCGLFNYQDPEFLESKKVEFISLWENLDFIKPDVTLPDELFKALEVESHSLTALLNFLSNINIDSKIALDFAGFLSKRKTIISTIIPETKEIENLLNLVDQRILEIYLKIIHLIKNDVNSLVLIQAIKNFANLPNYEVVKNEKLDLWQIDDGFSNGLTSKFRGYEIDELNIKYSTPQSTKINSTDYSIDTVALPPGSIELNSVKSWLTTSTWIDELELTTLQPISPSLTNDFFITIYGDSQYSLKNNYSLPPSTVIVDTSIEILSLAFPYLSTKVQLSLIENMRSFILSKNINEATKTTVSINCSVAIHGFLSVAQSEGLKFDQSVGSSVLDVLRTLYETYPSPYLLNLNSDSIGIIISKTSLSEQIPIFIKKIVDDQQSGSRSFSSLVLAAIYKYNSSHFSQIFEILMKLASDPHPTVHSWTFDALSTLIEKHVAMNVGKAKEMISMLEMFFLDDNYGAFNKSAAMSNLNVNVDSNKVLAKLLRHIINSLGPGVKDLDEVSKQTLQNLINGLLFYYNDEVIQSEIIKLVQELIIYDINAIDKQILIDLIEFNISNNLVNVIGTGLTLPLYGEKNEIFPVTSSFKVFGVSLDLLFQIVKTSEDKQFLSQVEYLIWIALETYPESTLLNKMIKEWLDSTYDISWFAKLQRLFNVSKRTLHEKLFNNYKAILSKYTHKKVEVDVNDEEAQSIAKKGEGEEEDSKSNESVNWKFKIVLLGYIRQLLGYTQRDSKLYTQLSSKVSDLIKISFSSSTSTINELRLLGIELLGDVINNYASAKDPIYPTMSLLEQQQAQITSALVPAFHYDSTPIIASKAITVVANFIGSRIVKINRLGRILKILTDSLDDLHESDDFKLQDVHITSIRGQRRVKLSVLNAWAELKILATSNEDAELNALIEEHLDVLLPLWVATLKEFAIIKHGSGDEHAEHDIELYERCWINFVDVVGCIVEVDETKIVDLLQNDSFGFFFMLYAQAIHSMIGHDHMKVRTLKALSKVLSYKKLVELILQDEIFSESIEVFERLILTGNIEEQSEILNITSKIFLNFFESSLSREELESYVDKLFELVRVNIQSIIRLVPYLQPENNRISTEVEELNPSEQQLLKKSFALINKMLARFPDMIKVDLYSSLLKLVLLVYEDHDRLVLRNQVIPSILPILKSLLQELVDMNDMVTINNFYQSIRPHLKVSQDVTSNINSLLSMGVLLSTSNDILRLNDDDIDFIATNLANGLIDDNTVAIATQSIKSVISKKSKLNEAIVKSLIPKILEHLLKGTISDPRLSIELFILTIKQVQSEEKQIPLFTILIPTLIFISNKSPDYTTYLHKKMISLIQLNPQVFKTVVNEILTPDQRVETEKLVKLEGQATSLEQVEHEQIRLKTFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9Y7</accession>
    <name>A0A1E3P9Y7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_15717</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019180656.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9Y7"/>
    <dbReference type="STRING" id="683960.A0A1E3P9Y7"/>
    <dbReference type="GeneID" id="30197902"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF33">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="AMP_BINDING"/>
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    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
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        <location>
            <begin position="70"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ62229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="666" mass="74714" checksum="6E02A437ABBDAC9C" modified="2017-01-18" version="1" fragment="single">QAVKLPNSKEEGHSSIYRSSHSPSQLVSTAHPRLNTAFNIFEVACELYGNQELFGTRLFNSETGKYDDFYTFQTYPQVRKRRNDLGSGIMNLFPNKRDFVVSIFSPNRPEFLITEHAASAYSLPMTSLYDTLVPKTTEYILNLTESPILVCPIDKILKILSIVASHDIPHLKYIISMDPINFEKDFGIIQLAKENHISIMSIDQLEAIGAKNPIPFIPPKPETLFAISFTSGTTGNPKGVKLSHKSVSAAIGGCISLISKPLSTLKEGEVPTAYSFLPLAHIYELLTGNLSIIRGFRTCFPNRPSDPANFVENMKICKPHFINLVPRVLNRFESVLKQGLSSTMQGRYLLSLLMGDSIPDWLRKSLQTKTRKTLGFDRVSFTICGSAPISPHTITFLNKILGIGYRQGYGMTESFSFTSASSVADPDYLNCGGVSITQELRLKDVPDMGYLTVDENGNELEEPRGEILLRGPQILDGYYKNPEATKETLNDGWYSTGDVGHVDKLGRIYVIDRVKNFFKLSQGEYVSPEKIESVYMSECDYLSQIFVAGVSIERYLVGVVGIDPIAIRKIFKKLSHVHGDNELVTKINKSTELKKDILHLMNKNVSHELQGFEKIHNLIVAIEPLKIEDETITPTFKVKRPNANKFFKKQINDMYSQGDLLRGEKI</sequence>
</entry>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="179" mass="19319" checksum="EEC18F303C8E9973" modified="2017-01-18" version="1">MGGRKYSGGSEGRPSRKRFSDQNGSTNGGGRFQRRGSKGGKRSDTSAGLKQEKHSPEACSRSSTSSQGSVDFAAPEKLEDQVDNGGAKAEYFRLFKIIKRSKSIPLKGDVCFELRPNRSFLMLVQLCTSGAREVVNHSNGLPRCCFEGEIFGDNSLASGSNTVQAIGLGIRLKEMLAHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAK8</accession>
    <name>A0A1E3PAK8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
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    <gene>
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    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Wickerhamomyces</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        </location>
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            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="19196" checksum="A6093A142F9A4626" modified="2017-01-18" version="1">MKAVYVALCCFVINVLANTEAFYVKLPNVQLESKQSYEQITIDEVNKVKFTVPSPNSQNHSYKWFKISGGNPGDIYNARFSWSALDPINVVHFDFETLNEHNESGLFLYLETKYDCYSKDQQFMNSEHNVEAKISYMVMCLTSSNIVEELYPKIEKVFTETTIVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAS9</accession>
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            <fullName evidence="12">Thioredoxin domain-containing protein</fullName>
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    <organism evidence="13 14">
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        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Wickerhamomyces</taxon>
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    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the SCO1/2 family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ62400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019185378.1"/>
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    <dbReference type="OrthoDB" id="270009at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:mitochondrial inner membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:copper chaperone activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:copper ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02968">
        <property type="entry name" value="SCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000013">
        <property type="entry name" value="Blast:Protein SCO1 homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003782">
        <property type="entry name" value="SCO1/SenC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017276">
        <property type="entry name" value="Synth_of_cyt-c-oxidase_Sco1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151:SF5">
        <property type="entry name" value="AT19154P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151">
        <property type="entry name" value="ELECTRON TRANSPORT PROTIN SCO1/SENC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02630">
        <property type="entry name" value="SCO1-SenC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037736">
        <property type="entry name" value="SCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="12">
        <location>
            <begin position="48"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="10">
        <location>
            <begin position="86"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010996"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR037736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037736-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR603782-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ62400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="234" mass="26685" checksum="B7430F75AE91F223" modified="2017-01-18" version="1">MKSSGNIEFASWKAAVLMVTTGGILYYFFTREKRRLEVQQQAEANRGYGKPLVGGPFELIDQDGKPFTQENLKGQFNILYFGFTHCPDICPDELDKLGLWLDKLKSQGIKVQPVFITCDPNRDTPKVVKEYLADFHPDIIGLTGTYDQVKNTCKQYRVYFSTPPNVKAGQDYLVDHSIFFYLMDSDGEFVDALGRNYDQDTGPVKIKEHVDAFVPKAEREKRKNGGSWFSSFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAV0</accession>
    <name>A0A1E3PAV0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_60600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ62546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAV0"/>
    <dbReference type="GeneID" id="30200268"/>
    <dbReference type="OrthoDB" id="5427212at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="360" mass="40531" checksum="66C4E4FE34BA44CE" modified="2017-01-18" version="1">MSGPEEAPYPKRQKRNSDGDHSVEQDVDQEPVVAQSKPSATTIKKANEIFDIMRTKRLSMADFVASLPYVSGNKRRQEVIVQSLFQNEEVLMELLFRNNFDVDFNPLCKVIGVKILKELGNIQQSNKLFGKFQVGDVENKDQLKSKILQSLEIDNNSVETLYKQAPILTNLFKELLPSRPEGETNSTTSVSNIDNRLILILSVICYSRNKKRCTNLPVLFGLYLNSLGLTTKRGLQVFNKFGISVNQKSIIDILDELSRLETARKDQGSKDQTVNSQVPNPENNSNTTRPPLNGPLVLPFTQNIISSTTDAYKPTSSEVLKISDAKFDQLFDQYTNNTTTDNIDHIDPKISNEMQQSKSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAZ8</accession>
    <name>A0A1E3PAZ8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_26287</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041803.1">
        <property type="nucleotide sequence ID" value="XM_019181242.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAZ8"/>
    <dbReference type="STRING" id="683960.A0A1E3PAZ8"/>
    <dbReference type="GeneID" id="30198488"/>
    <dbReference type="OrthoDB" id="6407164at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000400">
        <property type="entry name" value="Negative regulator of differentiation 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018885">
        <property type="entry name" value="mRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF10">
        <property type="entry name" value="RNA-BINDING PROTEIN NAB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10567">
        <property type="entry name" value="Nab6_mRNP_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="116"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="259"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="350"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="447" mass="50269" checksum="C4AE59A1EC738756" modified="2017-01-18" version="1" fragment="single">MATRVSNFQVSRQPTRSILLLNVNQDLSLTEFLDTITFGPIENVRIVENYPDEDHNSIALAFLKIETCLNFYNNILTYLSKFKKANKSPDLSINFINTKKLLPFISNAINSDGATRNVYVGNLKSLDKEIDEDFLYQEFSKFGIIDKIDLITKKTDEKTDQEAEEVEDEQNDQFAFIHFTNISSAIKAVEQLSLSSNWQDVKVFYGTDRCSINSTSSLIRYGSQQFMTDEDISNALNQKNLTAQAVATNAGGANNVGNRTVYLGNLDPKTKPEDICNVVRGGVLQYIKHFSTKKICFLTFIEPVAAAQFYANANIDGVTLHGRKLKIGWGNHSGPLPNPIALAVTIGASRNVYIGIKDQDLSDESLKLPSEEILREDFSKFGEIEQINFFKNDRCVFLNFLNISNAIKVVDDANGNNTEKFHDYFNGKYRNFKISFGKDRCGNPPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBA0</accession>
    <name>A0A1E3PBA0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Amine oxidase</fullName>
            <ecNumber evidence="11">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_50413</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="11">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <text evidence="10">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="10 11">Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the copper/topaquinone oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="11"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041892.1">
        <property type="nucleotide sequence ID" value="XM_019182652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBA0"/>
    <dbReference type="STRING" id="683960.A0A1E3PBA0"/>
    <dbReference type="GeneID" id="30199898"/>
    <dbReference type="OrthoDB" id="5379943at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009308">
        <property type="term" value="P:amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.20:FF:000001">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.20">
        <property type="entry name" value="Copper amine oxidase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000269">
        <property type="entry name" value="Cu_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015798">
        <property type="entry name" value="Cu_amine_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036460">
        <property type="entry name" value="Cu_amine_oxidase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016182">
        <property type="entry name" value="Cu_amine_oxidase_N-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638:SF33">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638">
        <property type="entry name" value="COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01179">
        <property type="entry name" value="Cu_amine_oxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49998">
        <property type="entry name" value="Amine oxidase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54416">
        <property type="entry name" value="Amine oxidase N-terminal region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0186">Copper</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 11" id="KW-0801">TPQ</keyword>
    <feature type="domain" description="Copper amine oxidase catalytic" evidence="12">
        <location>
            <begin position="236"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via topaquinone" evidence="9">
        <location>
            <position position="391"/>
        </location>
    </feature>
    <feature type="modified residue" description="2',4',5'-topaquinone" evidence="10">
        <location>
            <position position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ62685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="664" mass="75308" checksum="437C475D0EB89D97" modified="2017-01-18" version="1">MAALHPFDPITEAEIRLATKLVKDLNGSNYVHFTQLDRVDPPKKETLKYLDIERYGGGELPDIPRRVYAYYYLNDQMPVYKALVNVSYGHIITNQAIGDGACGPQLPDDLKDMEAVLYDHPAVQAEIKKLRLPSHIHVCCDTWMYGTDSDDVEVPLIQGYMYLKVDHPDANHYSIPLSFSPVFELLSKKFVRMDFLPAGFDEKTIGTQPWLEVKAVEYHPDLNGESTLRDIKPLIVQQPEGASFTIEGSKVKWQGWEFRVATNAREGVVLYDVHFKGRSLFYRIALNEMTVPYGDPRAPYHRKQAFDLGDCGFGVNANQLSLGCDCLGVIKYLDTLRVNRDGEPVLLNNTVCIHEQDYGILFKHMNYRNGNSIVTRRREFVIQTIATVGNYEYIINFVLDQVGAITVQVRATGILSTMPIEPGVVVPWGTNIGDGVMAANHQHLLSFRFDCRLDGEQNTACYDDYVPMEENTSLNPYNVGYVGKRTILEKSGHIEQSPFTNRTYKVINENVINRVTQKPVGYKFEMPARQMMIASPNSYNTKRAKFATKQFWVTKYQDDQLYAAGEFTNQSRTDTGLSKWANGEDSVRNTNVVVWPTLALTHPPSTEQFPVMPSDFMQFLVSPSSFFERNPALDVPLATNSFNKSKYYEQGAQEQDKSCCKTNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBL8</accession>
    <name>A0A1E3PBL8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Casein kinase II subunit alpha</fullName>
            <shortName evidence="11">CK II alpha</shortName>
            <ecNumber evidence="11">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_26686</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Phosphorylates YTA7 during S-phase to promote transcription of histones.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="11"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041559.1">
        <property type="nucleotide sequence ID" value="XM_019181289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBL8"/>
    <dbReference type="STRING" id="683960.A0A1E3PBL8"/>
    <dbReference type="GeneID" id="30198535"/>
    <dbReference type="OrthoDB" id="10254671at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007535">
        <property type="term" value="P:donor selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006356">
        <property type="term" value="P:regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14132">
        <property type="entry name" value="STKc_CK2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000459">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000088">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF27">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="43"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ62352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="333" mass="38887" checksum="C247642FE1233E0A" modified="2017-01-18" version="1">MSYPKAERVYSVSKVYADANEKRPKEYWDYENFEIEWGLISNYEIVSKIGRGKYSEVFQGLNIVNNQPCVIKVLKPVKIKKIYREVKILQNLTGGPNVVALLDLVRDAHSKIPALIFEEVKNVDFRTLYSKFKRSDIQYYFTQLLTALDYSHSMGIIHRDVKPQNIMIDPFNKKLRLIDWGLAEFYHPGVDYNVRVASRYHKGPELLVNLNQYDYSLDLWSVGAMLAAIVFKKEPFFRGDSNFDQLIKIAKVLGTDELYSYLKKYGLKLSKEYNDVLLPCPRRPWSSFVTDSNKYLVDDEIVDLIDHLLRYDHQERLTAKEAMAHPFFKGTPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC67</accession>
    <name>A0A1E3PC67_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_25004</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041684.1">
        <property type="nucleotide sequence ID" value="XM_019181084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC67"/>
    <dbReference type="STRING" id="683960.A0A1E3PC67"/>
    <dbReference type="GeneID" id="30198330"/>
    <dbReference type="OrthoDB" id="422637at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015867">
        <property type="term" value="P:ATP transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015916">
        <property type="term" value="P:fatty-acyl-CoA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042758">
        <property type="term" value="P:long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF69">
        <property type="entry name" value="PEROXISOMAL LONG-CHAIN FATTY ACID IMPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="439"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="703" mass="79796" checksum="928724AF32FD88BC" modified="2017-01-18" version="1">MSLALGNKGFDYRALGKFYSRNKNSILKASYLLLLFLSFNTSRPSRKKSTEKVDEAKETEIKKKTKLDNATVKRIMKAIIPDFKSPVIFYFILQLLLLAARAVLTLKVAALDGLLVSSMIRKKTSKFLQLLVKWMLLGIPASFTNSFLDFTTNQLALNINKNLTNNLLELYLPSEKKHEETLVKKNPNYYTLIHMSSDDKVDDPNQRITTDVNNLSSALASLPNHLLKPSLDLILCSFELAKSGSAEGTLILGVIAHLTSLLLKILSPNFTKIAIERSRLEGLLRSLHSRVVLFSEEVGFLRGHSRELDILDRSYYELEKFTNYEIRARAVYDTATSFIVKYTWGAAGLVLCSTPIFLNKFAGIIDHNSSATFITNRRLLLSASSSLGMLIRSRKEIQQVVGYFKRLTEFKKTLIEIDTAAASTESSTKGVVKYDDNKIEFKDIPLITPAKITLAESLSFTINHGDHLLIAGPNGCGKSSLFRILGGLWPVTKGELTIPHFENVFYLPQRAYLSKSSLKDQIIYPSTEYKYKTNKELLEILENPYLEVKDIDPFATIRNWPEELSIGVQQRLAMSRLYYHKPRFAVLDECTSAVSPQMEQLMYAHAQSLGISVLSVAHRSSLWHFHNYILKFKGDGTYSFTKLDAKQRLEWENELIELNKFLRDVPSLESRLKDLRIAQESQEFKRTQSQLSMRKLSSAQLAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC89</accession>
    <name>A0A1E3PC89_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flavodoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_27497</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WrbA family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042195.1">
        <property type="nucleotide sequence ID" value="XM_019181379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC89"/>
    <dbReference type="STRING" id="683960.A0A1E3PC89"/>
    <dbReference type="GeneID" id="30198625"/>
    <dbReference type="OrthoDB" id="504689at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032126">
        <property type="term" value="C:eisosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031934">
        <property type="term" value="C:mating-type region heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160020">
        <property type="term" value="P:positive regulation of ferroptosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000001">
        <property type="entry name" value="NAD(P)H dehydrogenase (Quinone) FQR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010089">
        <property type="entry name" value="Flavoprotein_WrbA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01755">
        <property type="entry name" value="flav_wrbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002999">
        <property type="entry name" value="PRK03767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546">
        <property type="entry name" value="FLAVODOXIN-RELATED PROTEIN WRBA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546:SF23">
        <property type="entry name" value="FLAVOPROTEIN-LIKE PROTEIN YCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="2">
        <location>
            <begin position="5"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="200" mass="21337" checksum="B38C451371D3885B" modified="2017-01-18" version="1">MTKNVGIIIYSLYGHIVKLAEAAKAGVELTGAKATIYQVEETLSPEILAKMHAPPKPDYPIATPETLTEYDAFLFGIPTRYGNFPAQWKTYWDKTGGLWASGALHGKVAGVFVSTGTPGGGQEVTVLNSLSTLAHHGIIYVPLGYKNTFPLNTSFEVVHGGSAWGAGTFAGADGSRSPNEVELEMAKIQGKTFAETVTKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PD78</accession>
    <name>A0A1E3PD78_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_25804</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042044.1">
        <property type="nucleotide sequence ID" value="XM_019181194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PD78"/>
    <dbReference type="STRING" id="683960.A0A1E3PD78"/>
    <dbReference type="GeneID" id="30198440"/>
    <dbReference type="OrthoDB" id="1724687at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000554">
        <property type="entry name" value="Ribosomal_eS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047861">
        <property type="entry name" value="Ribosomal_eS7_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11278">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11278:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01251">
        <property type="entry name" value="Ribosomal_S7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00948">
        <property type="entry name" value="RIBOSOMAL_S7E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="189" mass="21420" checksum="BF4F1D2C87EF6E4F" modified="2017-01-18" version="1">MSAASKIISENPSELELQVAQAFVDLEGNSPELKADLRTLQFKSIREIEVTGGKKAIAIFVPVPSLSAYHKVQTKLTRELEKKFPDRHVVFLAERRILPKPGRRSRQTQKRPRSRTLTAVHDKILEDLVFPTEIIGKRVRYLVGGNKIQKVLLDSKDSTSIDYKLDSFQSVYSKLTGKQVVFEIPGENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PDU2</accession>
    <name>A0A1E3PDU2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_61079</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042244.1">
        <property type="nucleotide sequence ID" value="XM_019183108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PDU2"/>
    <dbReference type="STRING" id="683960.A0A1E3PDU2"/>
    <dbReference type="GeneID" id="30200354"/>
    <dbReference type="OrthoDB" id="3941538at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003939">
        <property type="term" value="F:L-iditol 2-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006062">
        <property type="term" value="P:sorbitol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05285">
        <property type="entry name" value="sorbitol_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000068">
        <property type="entry name" value="Sorbitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045306">
        <property type="entry name" value="SDH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161:SF4">
        <property type="entry name" value="D-XYLULOSE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="8">
        <location>
            <begin position="14"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ63037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="363" mass="39629" checksum="13E48E0DEA82EFB1" modified="2017-01-18" version="1">MCQICLTNPSVQVTLDHRIEVKENSIPELKPGEVLINPRATGICGSDIHLWKHGQIGELKLHGNLVLGHEAAGDIVGVGDGVHNVKIGDRVAIEPGIPCHECFLCRQGDYNLCQSVKFIGIYPNPGSMQRYLVHDARYVYKIPSNMSYSQGALVEPVSVAYHGVTRAQLVLGQGVLIAGAGPIGLVTLLLTNASGCTPIVISDLSEKRLSFAKRLVPHAKTYKFDPKITSQLNAANIRNLFGPGEENAPSHVLECTGVESSIITGAYATRRSGTLVIIGVGKDIINNFPFMHLSFAEIEKFINRYHDTWPTVIKMISKKIINVDQLVTHRFPMEKVQEAIELAGNPSKGSIKVIIEDNANLHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUX6</accession>
    <name>A0A1E3NUX6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_65262</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036206.1">
        <property type="nucleotide sequence ID" value="XM_019183882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUX6"/>
    <dbReference type="STRING" id="683960.A0A1E3NUX6"/>
    <dbReference type="GeneID" id="30201128"/>
    <dbReference type="OrthoDB" id="2014654at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000791">
        <property type="term" value="C:euchromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051219">
        <property type="term" value="F:phosphoprotein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035753">
        <property type="term" value="P:maintenance of DNA trinucleotide repeats"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031930">
        <property type="term" value="P:mitochondria-nucleus signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000191">
        <property type="entry name" value="Retrograde regulation protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050273">
        <property type="entry name" value="GppA/Ppx_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003695">
        <property type="entry name" value="Ppx_GppA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057512">
        <property type="entry name" value="RTG2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30005">
        <property type="entry name" value="EXOPOLYPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30005:SF0">
        <property type="entry name" value="RETROGRADE REGULATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02541">
        <property type="entry name" value="Ppx-GppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23566">
        <property type="entry name" value="RTG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ppx/GppA phosphatase N-terminal" evidence="1">
        <location>
            <begin position="35"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="RTG2 C-terminal" evidence="2">
        <location>
            <begin position="350"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ56999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="568" mass="62948" checksum="9DA7BFF23A6987FB" modified="2017-01-18" version="1">MSSISPNDTEDEADVVGRALIGIVDIGSNGIRFSISSKASHHARIMPCVFKDRVGISLFDAQYDTNSTEKKPIPKDTINDVCAAMKRFRLICEDFGVQESGVRIVATEATREAVNSQEFRDAIYDSTGWEVELLNKEDEGRIGSYGVASSFDSLSGLFMDLGGGSTQISWIQCINGEITLSSNPVSLPYGAAALTRRLQFEDKKSLYFEIQNAYKEAFKKIDVPPQLLEEAERNRGFDLYTCGGGLRGLGHSLIADIKEYPIPTIINGFSCSFNDFIGISNYLFLKDSLPQKESIFRVSERRRSQLPAVGLLVSAACDSLPKIKTVHFAEGGVREGALYSMLPREIRAQDPLIIASRPYAPLLANKYLELLKTALPEKYVPKEVRERVAPALCNLAFVHCAYPKELQCTAALHVATTGIIAGSHGLSHRTRALIGVALCDRWGGDLPESEEKFKTSLCDLVKRDGNKIEMERIVWWTKYCGTIMYVICGVHPGGNIRDGAFNFKIVNGDMEVKDEDEKRKFDVIVEISNDDIKTSASVRGRILTLQKKVRKLSRGSSEKVKIGVQIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUZ3</accession>
    <name>A0A1E3NUZ3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Magnesium-dependent phosphatase-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_36037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036226.1">
        <property type="nucleotide sequence ID" value="XM_019182389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUZ3"/>
    <dbReference type="STRING" id="683960.A0A1E3NUZ3"/>
    <dbReference type="GeneID" id="30199635"/>
    <dbReference type="OrthoDB" id="2865258at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030946">
        <property type="term" value="F:protein tyrosine phosphatase activity, metal-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010033">
        <property type="entry name" value="HAD_SF_ppase_IIIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010036">
        <property type="entry name" value="MDP_1_eu_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01681">
        <property type="entry name" value="HAD-SF-IIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01685">
        <property type="entry name" value="MDP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17901:SF14">
        <property type="entry name" value="MAGNESIUM-DEPENDENT PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17901">
        <property type="entry name" value="MAGNESIUM-DEPENDENT PHOSPHATASE 1 MDP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12689">
        <property type="entry name" value="Acid_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01131">
        <property type="entry name" value="C1.5.2:_MDP_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="20552" checksum="5EF4C0AA6C7F07DF" modified="2017-01-18" version="1">MTQYPKVVVFDLDYTLWLANYIQKCWCDTHISPPIKAKSTKTLVDKYGFQISFYKDVEEIFKELKENNVYIVAASRTSAPALARKMLKSLHLNGEPAINHFDALEFGTFSKKNHIKSAMSSLDQEIELKHVILFDDELRNKDVETLGVQFAHIFDEDRGLTKQIFQNAIAKYNNRLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV48</accession>
    <name>A0A1E3NV48_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tafazzin family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_36009</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1'-[1,2-diacyl-sn-glycero-3-phospho],3'-[1-acyl-sn-glycero-3-phospho]-glycerol + a 1,2-diacyl-sn-glycero-3-phosphocholine = a cardiolipin + a 1-acyl-sn-glycero-3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:33731"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="ChEBI" id="CHEBI:62237"/>
            <dbReference type="ChEBI" id="CHEBI:64743"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:33732"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:33733"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Mitochondrion outer membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the taffazin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036182.1">
        <property type="nucleotide sequence ID" value="XM_019182386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV48"/>
    <dbReference type="STRING" id="683960.A0A1E3NV48"/>
    <dbReference type="GeneID" id="30199632"/>
    <dbReference type="OrthoDB" id="193467at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035965">
        <property type="term" value="P:cardiolipin acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097250">
        <property type="term" value="P:mitochondrial respirasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000872">
        <property type="entry name" value="Tafazzin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12497:SF0">
        <property type="entry name" value="TAFAZZIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12497">
        <property type="entry name" value="TAZ PROTEIN TAFAZZIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00979">
        <property type="entry name" value="TAFAZZIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="71"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010524"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00024323"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047906"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU365062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ56975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="407" mass="46954" checksum="6BF5E940E8D40D6B" modified="2017-01-18" version="1">MSLQAVLERGDDILGQFPRDSSFWNYASQATCVLAVGWSKLVLQTCYRPRITGVEKLDRALERSQRENRGLMTVMNHMSVVDDPFVWGFLPWRFYRDVDAIRWGLGAHNVCFQNKFLTCFFSLGKILATERFGVGPFQGSIDAAIRIMSPDDTLDLVYNGDDDETNSNWLDPNHYKSELNKLTPLAQKINNDYIPPIKRSRPSWIHVFPEAFVLQLHPPHANSMRYFKWGITRIILESTRQPIIVPIFATGFEKIAPETAAGGSLIERFLPANFGCEINVTIGDPIDDSIMENFRKEWLDLVKSQKTSMDLNDELKFGEKAQLLRSKVASELRKAVADIRHQIGFPDEDPRFKDHKFWHEFTKTEGKSDPNVQFVGQNWAIRRLQSFLKEHDEYELNKNHENGEEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV81</accession>
    <name>A0A1E3NV81_WICAA</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_65371</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036318.1">
        <property type="nucleotide sequence ID" value="XM_019183900.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="30201146"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="123" mass="13781" checksum="A1543805A5F37691" modified="2017-01-18" version="1">MSSDLSSEEDHFDNVKKMYNQALNKVVEGDDETDDSDYVPGDELQIDEDSIDSYDYHLSSKKRPWSDVNEPEEIEESKEVNYKVLHKKQRTEGQLKRTLIAGTVGAATGAVATFFGLVFAGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVR4</accession>
    <name>A0A1E3NVR4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_97795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019186608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVR4"/>
    <dbReference type="STRING" id="683960.A0A1E3NVR4"/>
    <dbReference type="GeneID" id="30203854"/>
    <dbReference type="OrthoDB" id="3981296at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044615">
        <property type="term" value="C:nuclear pore nuclear basket"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031990">
        <property type="term" value="P:mRNA export from nucleus in response to heat stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034398">
        <property type="term" value="P:telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034432">
        <property type="entry name" value="Nup60"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28284">
        <property type="entry name" value="NUCLEOPORIN NUP60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28284:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="447" mass="50041" checksum="B2BC5FA5F1914EE3" modified="2017-01-18" version="1">MFSPSAWLSENESTIGDDSDASFASELNSIPPSSIQQTPKNPIDEFHRMQHQKIKDSPNARLADFFAKKGTEPLSEIELEGVRALLEKSNSVQTSPKTPRNESFNSEDQIEILKPKNTESAVKNTPSFKATYNTTVSAENSFDNSVASSNITSSRKRRVFDFSGFPSPYRTSRLKESSFMLEEDKPEVSTEANVEPPKEEKKLSNTASALLSFIENHETTEESNEKKQDTQSKKKVDYSNPYATISTSHKKTKKPASRKPTAFEQLEKSLSAAPQPELPNINKYKPAKSSSLRESISLNDTTDQSVEEIEVDEPEQEALKPQVPSFSFKAPEPPKEQDKPPIPTENPSEPKPTFNSKPLFSFAPKPTTASFSPESTIQTENTQLQKRDTQISPRPKQFSFSGSVVNQDTRPPSSDFTFRFPNAEPLGYKSADINEQKVAHFKTYFTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVR6</accession>
    <name>A0A1E3NVR6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_65496</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036445.1">
        <property type="nucleotide sequence ID" value="XM_019183921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVR6"/>
    <dbReference type="GeneID" id="30201167"/>
    <dbReference type="OrthoDB" id="3980711at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="139" mass="15766" checksum="74B31D7135ED986E" modified="2017-01-18" version="1">MSLLNSLYSLVHPGQERSISFLSHNNTPVNNFCSIIPIVQPSLISTSFGQSIITLSILLLYLSLYLSYANTHKGVEFRILTSLILGNLVFTILSFNDWFNQFYSIVFCNDYKNGVLNLYQVSELIETYKLGNLIKFIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVR9</accession>
    <name>A0A1E3NVR9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">asparaginase</fullName>
            <ecNumber evidence="1">3.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_18382</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036468.1">
        <property type="nucleotide sequence ID" value="XM_019180785.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVR9"/>
    <dbReference type="STRING" id="683960.A0A1E3NVR9"/>
    <dbReference type="GeneID" id="30198031"/>
    <dbReference type="OrthoDB" id="542841at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009066">
        <property type="term" value="P:aspartate family amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500176">
        <property type="entry name" value="L_ASNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="L-asparaginase N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="3">
        <location>
            <begin position="185"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="298" mass="31589" checksum="CFF97496F2AA7230" modified="2017-01-18" version="1" fragment="single">GIGIGSLLESAGLDNVATLSYEEIANVSSADLNSTYLIDLYNKVNDDLSSDEFDGAVIVQDSQYLEDTAFFLDLVVNSSKPIVLTKDVSISNLLTEGASNLYDSVLVAASDLAANRGTLVVTQKRIASGFYAKRINFDSIDAITSNPQGLIGNIYEGKVQWYYDESDLLTSDLDFTLDEVDSLPDVVVLQNYQGFSSGIISALGESSIDGVVLGSSQSTDSFSKDVEKAIEGLAQSVPVVLSNDQPGSTVSPDDLFSNVTIAGGILDPVKSRILLQVSLAAGYNSTEVKDIFSTVFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVS2</accession>
    <name>A0A1E3NVS2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aldehyde dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_86140</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3 6">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036422.1">
        <property type="nucleotide sequence ID" value="XM_019186001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVS2"/>
    <dbReference type="STRING" id="683960.A0A1E3NVS2"/>
    <dbReference type="GeneID" id="30203247"/>
    <dbReference type="OrthoDB" id="440325at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000004">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF16">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE TYPE III, ISOFORM Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="7">
        <location>
            <begin position="11"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="active site" evidence="4 5">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR036492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="468" mass="52099" checksum="C928C1C4D61B1537" modified="2017-01-18" version="1">MSNVLEYTPVDDIPSIVTSLHKSFRANPNRSLEYRLNQLRNLYYAIKDNEELLVRVLKKDLNRPEGDTQLLEITPLYIEILTTINNLSKWMKPTSAKNVDLLYYFTSPKIAKIPLGTVLIIGPFNFPYLTTLAPLVGSIAAGNTTVFKQSEQIPYTSQTLGTILTDALDPKIFQIVQGGIEETTVLLNQKFDKIIYTGSGKVGRIIASKAAETLTPTILELGGKSPAFVTPSVQPKDLKTVCKRIAFTRFANAGQICVSTDYCIVHDSLYDEFVDMMKLVIKELYDVDETNSTKLVNKNAWDRVMNIIRNTKSDVVLGGDGDREARFIQPTLLTNVSFDDASMKEEIFGPVLPVIKYSDLQHSIDNVLDIADTPLALYIYSKSSKEIETISSQIRSGALVVNDGVIHGGLACVPFGGVGESGYGSYHGKWSFDAFSHERAVLKNPFWTDILFESKYPPITKMDLKLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVW0</accession>
    <name>A0A1E3NVW0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_58788</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036337.1">
        <property type="nucleotide sequence ID" value="XM_019182831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVW0"/>
    <dbReference type="STRING" id="683960.A0A1E3NVW0"/>
    <dbReference type="GeneID" id="30200077"/>
    <dbReference type="OrthoDB" id="3978754at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19079">
        <property type="entry name" value="AKR_EcYajO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000004">
        <property type="entry name" value="Oxidoreductase, aldo/keto reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050523">
        <property type="entry name" value="AKR_Detox_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364:SF15">
        <property type="entry name" value="ARYL-ALCOHOL DEHYDROGENASE AAD16-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364">
        <property type="entry name" value="NADH-SPECIFIC METHYLGLYOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="24"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="362" mass="40770" checksum="39E28CAD8A3FF0F8" modified="2017-01-18" version="1">MSSETTTVVPWKNLGKSGLKISNIIIGCMGFGSKKWDPWVIEDKQEVFKILKKAYDHGIRTYDTADNYSNGLSEKILGEFLKEYKIQRDKVVIMTKFWGPTDDAYPEGYKGIFVDYIGSPIDKINFLNNRGASRKHIMDAAKNSSERLGTYIDVYQLHRFDKDTPIEETMKALNDVVNAGYTRYIGASTMRAVDFVEMQHVAEKNGWHKFISMQSLYNLLQREDESEMNYYCDKTGVGLIPWSPLMGGVLARPVPKDESEKTERIKTSMFTVFFKSADLTIGTKGQDSDIEIVNRVGEIAKTKGTSMAAVATAWVIAKGAAPIIGFTTVERVDDAVEGANLKLTEEEIKYLEEPYLPRARVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVX2</accession>
    <name>A0A1E3NVX2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RTA1 like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_59028</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lipid-translocating exporter (LTE) (TC 9.A.26.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036470.1">
        <property type="nucleotide sequence ID" value="XM_019182833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVX2"/>
    <dbReference type="STRING" id="683960.A0A1E3NVX2"/>
    <dbReference type="GeneID" id="30200079"/>
    <dbReference type="OrthoDB" id="3358017at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007568">
        <property type="entry name" value="RTA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465:SF1">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04479">
        <property type="entry name" value="RTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="34645" checksum="555FC01E2F5071AE" modified="2017-01-18" version="1">MATERIVWRNYWYNPSLAGAIIFIILFFALTLIETFQAYKTSQATAQYNGSYRFVLILIPFHLGLYCEFLGYIGRTISHGNIEDQHSYIMQTLLLLIGPPFFSASIYMVFGRIVTNILHNENYLLLYPKYITKVCVVGDIISLLLQAAGGGIMANTKKNINNFALGENVILGGLAVQILFFGFFVIIEATYYIRLHKNMQSSEYNSRVINQEIRHFPNKFNDWKTILLTLFLCSIFILIRSIYRVVEFSQGNNGYIARHEWFLYFFDALMMFFNALLFVSQDVSNYFLKTIPLTLEVAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW15</accession>
    <name>A0A1E3NW15_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_80742</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036606.1">
        <property type="nucleotide sequence ID" value="XM_019185115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW15"/>
    <dbReference type="GeneID" id="30202361"/>
    <dbReference type="OrthoDB" id="4021219at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="375"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="283"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="333"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="433"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="454" mass="52282" checksum="B019C5B8B64B2533" modified="2017-01-18" version="1">MESLTVSEYVDPVLSVVGKEEKINPQTIQSLTSNFPSYYSKITSFCSSALKIYKNLLNLEYELNSWEFMTLKASININFNHNFSTTTRNFDIKIAKKVIDRCNFIDDEINKLLNYKLKIINNFISSLTIQEYLSDPGCLLLKIYFKIINLKNSIVEKISISYTKSKLLIISFELKQITDMIEKEENPIVEDNTDFQSTLEGYKDFVTVLINQLDQAVQEKDSDQIQECLEVLNDVEKMYESVRLNFFINEEYAEWEQDNYYIQSNRERELEQELAKERELSRSRSISPSPSSQTSLNEELLSSSTSPPSNSSSKFLNLHSSQELPGHHHLSRRGSISSTTSSVATLNRQPTTISEELPYLLQAFDEAKQLEQELSTYQTPRTPTSSQQQQQQQAMKKLQPIHNQDKRRSNLQTFNIPNVGFNNNLLNSIYGLTPKSSTPSTPSHLNHHHHNEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW70</accession>
    <name>A0A1E3NW70_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Bicarbonate transporter-like transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_64718</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036572.1">
        <property type="nucleotide sequence ID" value="XM_019183790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW70"/>
    <dbReference type="STRING" id="683960.A0A1E3NW70"/>
    <dbReference type="GeneID" id="30201036"/>
    <dbReference type="OrthoDB" id="1735926at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080139">
        <property type="term" value="F:borate efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050801">
        <property type="term" value="P:monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000003">
        <property type="entry name" value="Anion exchange family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF82">
        <property type="entry name" value="BORON TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="453"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="477"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="8">
        <location>
            <begin position="27"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="8">
        <location>
            <begin position="204"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="518" mass="58140" checksum="2722EDFB4A08E1B1" modified="2017-01-18" version="1">MIEDIEEKPQAEESKPTLKWKDRYKIRLFRGIILDLKDRLPLYWSDWTDALNYRVIPATILTFFTNIFPAIAFAQDMFDKTDNSYGVNEVLLSSALAGIVFGIFGGQPLTIVGVTGPITIFNYTVYEIMKPHDIPYFPFMFWIAIWGMIFHFIISITNCVSALRFVTQFPCDIFGLFINVVYLQKGVQILTRQFKPEDNGASGFASCTLAISMGVLGFAGNCFNETKYFNHHIRQFIVDYTTPLAMVFFSGFIHFGGYLNNVEFERLPITKSFHPTSRLHWIDVTGISVGSIFLALPFGIILAILFYFDHSISALMAQDSKFKLKKPAAFHYDLFLLGIVTGIAGVLGIPCPNGLIPQAPLHTESLVVRNRANVILGTVEQRFTNTVQGLMLLGTMTRPLLVCLGLIPQCVLAGLFFIMGAKGLHGNVIINRIRFIFSQDHSSDLASVPKKKLYLFICFSLIGFVAEFGIVNTKGAIGFPLVLLLSVIGTFWFKWIFPKEELDILDGPVAHDNTLKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW72</accession>
    <name>A0A1E3NW72_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Maltose/galactoside acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_34896</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036581.1">
        <property type="nucleotide sequence ID" value="XM_019182250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW72"/>
    <dbReference type="STRING" id="683960.A0A1E3NW72"/>
    <dbReference type="GeneID" id="30199496"/>
    <dbReference type="OrthoDB" id="25818at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03357">
        <property type="entry name" value="LbH_MAT_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051159">
        <property type="entry name" value="Hexapeptide_acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024688">
        <property type="entry name" value="Mac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416:SF54">
        <property type="entry name" value="ACETYLTRANSFERASE, CYSE_LACA_LPXA_NODL FAMILY (AFU_ORTHOLOGUE AFUA_2G08430)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416">
        <property type="entry name" value="SIALIC ACID SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12464">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01266">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Maltose/galactoside acetyltransferase" evidence="3">
        <location>
            <begin position="29"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="223" mass="25159" checksum="83F8772FAA603D12" modified="2017-01-18" version="1">MTELFKKNQELIDYVYANFNGVPKGSDDYEKMISGMRYRCWVPELKYARMRRHERALKYGNISIDDFETPDEYEQARLDYLKTVFGNIKGEAFVEAPFYVDYGFNIEVGDKFYANFNLTILDGSIVKIGHGVALGPNVTLTCATHPLDPYMRVDDHYEWSREIIIGNRVWLGANVTVLAGVHIGDNVVVGANSVVTKDIPANSVAVGIPARVVKKLEVVERGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW81</accession>
    <name>A0A1E3NW81_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_65719</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036044.1">
        <property type="nucleotide sequence ID" value="XM_019183973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW81"/>
    <dbReference type="STRING" id="683960.A0A1E3NW81"/>
    <dbReference type="GeneID" id="30201219"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000001">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF3">
        <property type="entry name" value="AMINO-ACID PERMEASE PB1C11.02-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="366"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="395"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="10">
        <location>
            <begin position="48"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="8"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ56837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="539" mass="61043" checksum="8D4AA2F844FCA6C0" modified="2017-01-18" version="1">MDPKKQEILSTTVDSDLEKASTTSTNSKQNGDVENIKELNRSLSTRQVSMIAIAGVIGTGLYLGTGKSLAQGGPLSLLITYSIMSLEVYLMMLALGEMSAYMPIAGSFCTFAKKFGSESLGFAILINYWLNDAVSVASDLTALQLVMKYWTDFHFWIVSLIFWVLLLFLNIFHVRFYGETEYWLALLKVLSIIAFFIVSIVVNVGHNEQHEYIGFKYWSMGDAPFVDGFKGFAKLFVTASFALGGTESLTLTSGEMTNPVRSTRIITKTVFWRIVIFYIFSVFFIGMNVPYNYPNLLTKSVVTSPFTIVFQQAGSKSAGSFMNAVILTSVISAGNHALYAGSRLAFTLGTEGYLPKILARKNRFGIPYVAVLITWFAGGLCFGSSFIGAGDLWSWLQNLVGVSNQISWLCIGITSIRFRKGLAKQGRLHELQYKNWTYPYGPWFVIIFTSIILLVQGWTSFAPWNVSDFFSFYLELFVFPVCLLFWWVFHRKDRFKKAQEMDFDTDRYIESDEERELREKLDSLKGWAKVKRILQDFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWA6</accession>
    <name>A0A1E3NWA6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_21686</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion inner membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036487.1">
        <property type="nucleotide sequence ID" value="XM_019180912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWA6"/>
    <dbReference type="STRING" id="683960.A0A1E3NWA6"/>
    <dbReference type="GeneID" id="30198158"/>
    <dbReference type="OrthoDB" id="7813104at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042719">
        <property type="term" value="C:mitochondrial intermembrane space chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140318">
        <property type="term" value="F:protein transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="3 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0811">Translocation</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Tim10-like" evidence="9">
        <location>
            <begin position="29"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="93" mass="10208" checksum="4CC593952857F004" modified="2017-01-18" version="1" fragment="single">LFGGSTSTTPVTSSTVSSNSQVKDQLKAQIGQELAIANATELVNKITDNCFQQCQKQPFATGEENCTDRCLEKYMRAWNAISKTYIARIQQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWD7</accession>
    <name>A0A1E3NWD7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_80771</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036642.1">
        <property type="nucleotide sequence ID" value="XM_019185121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWD7"/>
    <dbReference type="STRING" id="683960.A0A1E3NWD7"/>
    <dbReference type="GeneID" id="30202367"/>
    <dbReference type="OrthoDB" id="428974at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF33">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G14860)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="109"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="390" mass="44436" checksum="35C64385FD5ACB9A" modified="2017-01-18" version="1">MSLEQYQQLAKQEQDEIPKSLIPKVIHNLKLLGHFLFNSKHTELETLLINDESTFASDLTQTYGFTQHYEQYGEIFCLKIPHPLRTQLNNSAISKEVVNELESKINEIPVIVFIHGLGGNLQQFDEVVRKFAGLTDIFAVDLPGSGKSKAKQGLKLTLENFTNFVKESLDTNGFIGREIIIVAHSYGTQVALKLAEINEINLKGLVLLAPPKIPLKRTWKENFFLGLFLKIPWLFEFFRKLDRFNNIQSLSMKRLFSNPETPEFLRLKQFRFNLLTNSTNFLNHAKSWIPLTISEVIDASQTISNNNGSILIVDGSDDKVTHDGGASYHDLLGNNISQYQILENAGHNFILESFETLNPLLEKFFVSLDSRLDSDYPNKLRSEFSKRSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWH6</accession>
    <name>A0A1E3NWH6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histidine kinase/HSP90-like ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_36584</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036239.1">
        <property type="nucleotide sequence ID" value="XM_019182449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWH6"/>
    <dbReference type="STRING" id="683960.A0A1E3NWH6"/>
    <dbReference type="GeneID" id="30199695"/>
    <dbReference type="OrthoDB" id="28737at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140453">
        <property type="term" value="C:protein aggregate center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16927">
        <property type="entry name" value="HATPase_Hsp90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.790:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.80:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11260:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000001">
        <property type="entry name" value="Heat shock protein HSP 90-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="entry name" value="Heat shock protein 90, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
        <property type="entry name" value="Heat_shock_protein_90_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003555">
        <property type="entry name" value="PRK05218.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="HSP90 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="10">
        <location>
            <begin position="27"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="215"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="673"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="518"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="676"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="693"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="100"/>
            <end position="101"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="120"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR002583-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ57032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="703" mass="80684" checksum="2F4829F29BEDBC2C" modified="2017-01-18" version="1">MSKAESYEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYEALSDKSKLETEPELFIRITPKPEQKVLEIRDTGIGMTKADLVNNLGTIAKSGTKSFMEALSAGADVSMIGQFGVGFYSLFLVADRVQVISKNNDDEQYIWESNAGGKFTVTLDETNERIKRGTILRLFLKEDQLEYLDEKRIKEVIKRHSEFVSYPIQLLVTKEVEKDAPEAEEEKKDEDDKKPKLEEVDEEEDKEKKEKKKVKETVTELEELNKTKPLWTRNPSDVTQDEYNAFYKSISNDWEDPLAVKHFSVEGQLEFKAILFIPKRAPFDLFESKKKKNNIKLYVRRVFITDEAEELIPEWLSFVRGVVDSEDLPLNLSRETLQQNKILKVIRKNIVKKLIETFNEIAEDAEQFDKFYTAFGKNLKLGIHEDSQNRTALAKLLRYNSTKSTEELTSLSDYVTRMQEHQKNIYYITGESLKAVEKSPFLDILKAKNFEVLFLVDPIDEYAFTQLKEFEDKKLVDITKDFELEETEEEKAQREKEEAEFENLAKALKEILGDQVEKVVVSHKLVDAPAAIRTAQFGWSANMERIMKAQALRDTSMSSYMASKKIFEISPRSPIIKELKNKVEADGAQDRTVKDLTTLLYETALLTSGFTLEEPTSFASRINRLISLGLNIDETEETSAAAEPEASTQAPSEEQVAETEMEEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWI4</accession>
    <name>A0A1E3NWI4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">leucine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Leucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_85649</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="2"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036705.1">
        <property type="nucleotide sequence ID" value="XM_019185921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWI4"/>
    <dbReference type="STRING" id="683960.A0A1E3NWI4"/>
    <dbReference type="GeneID" id="30203167"/>
    <dbReference type="OrthoDB" id="10249672at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903432">
        <property type="term" value="P:regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.740.10:FF:000001">
        <property type="entry name" value="Leucine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004493">
        <property type="entry name" value="Leu-tRNA-synth_Ia_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055416">
        <property type="entry name" value="RBD_LARS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00395">
        <property type="entry name" value="leuS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008957">
        <property type="entry name" value="PRK12300.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794:SF1">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24810">
        <property type="entry name" value="RBD_LARS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="11">
        <location>
            <begin position="55"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="11">
        <location>
            <begin position="198"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="12">
        <location>
            <begin position="806"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine--tRNA ligase RagD-binding" evidence="13">
        <location>
            <begin position="949"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="127"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF24810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ57498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1087" mass="123944" checksum="FC8B05D35922EAC2" modified="2017-01-18" version="1">MADAKTIVLENTARRDVLIDIEKKYQKIWADEKIFELDAPSLEEETVDIDIDELHAKYPKFMSSMAYPYMNGVLHAGHCFTLSKVEFSVGFERMRGKRALFPLGFHCTGMPIAACADKLAREAEQFGDDYTGAPPAEEEEEEVAAPKPQAKSEDVSKFKAKKSKAAAKQGRGKFQFEIMLQLGIKREDVVKFADANYWLEYFPPLCKKDCDSFGARIDWRRSMITTDVNPYYDQFVKWQITRLKDIGRIKFGERYTIYSEKDGQACMDHDRQSGEGVTPQEYTGIKIEVTEFADPSVAENLDKSKKVYLIAATLRPETMYGQTCCFVSPKIDYGIFDNGDYYFITTERAYKNMSYQKLTPKRGDYASVAVVNGSKLIGSKISAPLSIHKELRVLPMETVLATKGTGVVTCVPSDSPDDYITTKDLQNKPEYYGIQKEWVNEPVPLIRTEKYGDMCAEYLCNELKIQSPKDALQLAKAKEAAYKEGYYNGTLIYGPYTGEKVEVAKPKVKADLIAAGQAFSYNEPEAPVVSRSGDSCIVSLEDQWYVDYGEESWKADALEALHDMNTFTTETQNAFEGVLDWLKNWAVCRTYGLGTKLPWDEKYLVESLSDSTIYQSFYTISHLLHKDFYGREEGELGIKAEQMTSDVFDYIFLRKEDIKTDIPIEHLQRLRREFEYFYPLDVSVSGKDLIPNHLTFFIYCHTALFPKRYWPQGIRANGHLLLNNNKMSKSTGNFMTLEQIVEKFGADASRIALADAGDTVEDANFEESSANAAILRLTTLKEWSEDAIKRVDTFRTGEFDFFDIAFENELNELIEETYKQYDESNYKAALKTGLFDYQTARDYYRESCEVMHRDLVLRYIETQILLLTPIVPHFAEYIYREVLGKNTTVQDAKFPRATKPVDLSISEGLEYIRYLTRSIRETEGALMKKKKGKPSELDSSKPVKLTIFVSESFPEWQNNYIELVRELFEKQSLNDNNVIKQKVGKDMKRAMPFISNLKQRLTKESPSVVFNRELNFSEVETIKNALDNIKRASSIVKTKEVQVVSFKNGASKGKDVLTGEEVPILITGKIIDSAVPGQPGLLMTNID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWJ7</accession>
    <name>A0A1E3NWJ7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aprataxin C2HE/C2H2/C2HC zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_35671</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036702.1">
        <property type="nucleotide sequence ID" value="XM_019182342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWJ7"/>
    <dbReference type="STRING" id="683960.A0A1E3NWJ7"/>
    <dbReference type="GeneID" id="30199588"/>
    <dbReference type="OrthoDB" id="3512845at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033699">
        <property type="term" value="F:DNA 5'-adenosine monophosphate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120108">
        <property type="term" value="F:DNA-3'-diphospho-5'-guanosine diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019002">
        <property type="term" value="F:GMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905108">
        <property type="term" value="F:guanosine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990165">
        <property type="term" value="F:single-strand break-containing DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000012">
        <property type="term" value="P:single strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032566">
        <property type="entry name" value="Znf-C2HE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486:SF4">
        <property type="entry name" value="APRATAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486">
        <property type="entry name" value="APRATAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11969">
        <property type="entry name" value="DcpS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16278">
        <property type="entry name" value="zf-C2HE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aprataxin C2HE/C2H2/C2HC zinc finger" evidence="1">
        <location>
            <begin position="134"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="208" mass="24621" checksum="C35036358F7523F8" modified="2017-01-18" version="1">MSFRFALERYIKDPDSQGDAIIYKDEKYVVIKDKFNKSVIHYLLLPRDKNLTKLNPLSAFEDDILKKEVEELIRKVKVMILERLQREFGIEETEEFIKVGVHSVPSMANLHVHLLTKDMNSERMKHKKHYNSFTTKFFVDFDELPLKADDKSSEAHVMENLTKTSDLKCIYCGENFSNKFAQLKKHLTKEFEKKCRSKKRKIEVIEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWP4</accession>
    <name>A0A1E3NWP4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_81336</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036319.1">
        <property type="nucleotide sequence ID" value="XM_019185216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWP4"/>
    <dbReference type="GeneID" id="30202462"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="535"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="560" mass="63114" checksum="9B5BD39DC1221D7A" modified="2017-01-18" version="1">MTMILIGRKKTDKDCGERDQDDSSNGPIGSVVVDEDDLFTSTEQLTRDDIERVEETNQQADDSMITVESDEEAPEESPILKKSMGDADKDSDVEIIEDNNVQEIKKDNKKQTKAQNSNGIENGFDDYDEDEDEDYDYENEDSNHSKSQLFSSDEESDITPNHEPHVQLTADEITADLLKYVEMCSEKCGEALDDCSEAVLTSGETSDEDEEDHGYGFTTDLDLKTYDWNEEDEDADGDYHPYLPQDKYLDDSKEAVYSEGETSDEDTEDHGYVGHSSNVDLQNVDWDEDEDQDHPDEDYLPALPGDKILDDSQEAVYSNGETSDEDQEEHGFVRDDVNLGNYDWDEDSEEENADYDYLPALPNEHLLVDSQDAVYSDGETSDEDEVDHGILIDDNISLSALNNDIWDDDSDGSDFEYLPELSNIKVIKELDAESEVSMSSDLSSEEDHFDNVKKMYNQALNKVVEGDDETDDSDYVPGDELQIDEDSIDSYDYHLSSKKRPWSDVNEPEEIEESKEVNYKVLHKKQRTEGQLKRTLIAGTVGAATGAVATFFGLVFAGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWX6</accession>
    <name>A0A1E3NWX6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_21584</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036896.1">
        <property type="nucleotide sequence ID" value="XM_019180908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWX6"/>
    <dbReference type="STRING" id="683960.A0A1E3NWX6"/>
    <dbReference type="GeneID" id="30198154"/>
    <dbReference type="OrthoDB" id="4207594at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd21615">
        <property type="entry name" value="RRM_SNP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000132">
        <property type="entry name" value="Small nuclear ribonucleoprotein U11/U12 subunit 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051183">
        <property type="entry name" value="U1_U11-U12_snRNP_70-35kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022023">
        <property type="entry name" value="U1snRNP70_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952:SF5">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12220">
        <property type="entry name" value="U1snRNP70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="104"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="204" mass="23504" checksum="5A8963D59E0089FA" modified="2017-01-18" version="1" fragment="single">DTSKFSADVAKLFEPRPPLRYVEQIDTPPSDRQTAHISGIGSFLDKLKEYKQVEFPKEEFIETPQLKEQRLKREKQLLNKKKLDEDFANWDPSTDPHIKGDPYNTLFVGRLDYEVTEIDLQKEFSKYGPIERVRVVRDKETGKSKGYAFIVYERESGTRAAYKEANGLKIKGRSILTDVERSRTSKSWVPRRLGGGLGGRGYTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX08</accession>
    <name>A0A1E3NX08_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">High osmolarity signaling protein SHO1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">High osmolarity signaling protein sho1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 12">Osmosensor SHO1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_35226</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SHO1 family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ57625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019182285.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044697">
        <property type="term" value="C:HICS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:osmosensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:osmosensory signaling pathway via Sho1 osmosensor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:signal transduction involved in filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="SH3_Sho1p"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="High osmolarity signaling protein SHO1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035522">
        <property type="entry name" value="Sho1_SH3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF20">
        <property type="entry name" value="HIGH OSMOLARITY SIGNALING PROTEIN SHO1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="9" id="KW-0346">Stress response</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="254"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009739"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00016255"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ57625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="314" mass="35216" checksum="8CD1AF78767B5180" modified="2017-01-18" version="1">MGAFQKPQSSRSNFQFSNISTDPFAISTISIALISWIIAFGGAIAASLVKDPNNSFPNFSWWGIVFELVLLIMLFCFYTFDLIDQYKIFLTSAFGIAFVYTTNSATSLVYSSGSGKAAASAGVILLSMVNLIWVFYYGGDNTSPVNQWIDSFSLRGPRSSNNPFAHHNQLHGNYNGFGVSNNNVDDYRGSTVSNQRFVSSTRLNGLENLSERNTDTNNTPMYENIIPENHDDNNDNINTNISNEQFDTNLMIDDFPYTARALYSYESSPEDENEISFEKGEILKVNDIHSRWWQAKRSNGEIGICPSNYVELIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX72</accession>
    <name>A0A1E3NX72_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_34902</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ57570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="30199497"/>
    <dbReference type="OrthoDB" id="2121326at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.470">
        <property type="entry name" value="PITH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010400">
        <property type="entry name" value="PITH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037047">
        <property type="entry name" value="PITH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46115">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06201">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="PITH" evidence="3">
        <location>
            <begin position="134"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="323" mass="35996" checksum="C933D272FF498E47" modified="2017-01-18" version="1">MSIPYIASPDQFQSALQRSEALIVYFTAVWCGPCQAIAPIVEKLYTSFTNIDVFKVDLDSNKALASQQSISAVPTFILFHKGKEISRIQGANIQALNEGFKKLSDLAPNAKRQAVSSTNESSSSSGGSNIDATIAKYVPKGFQILNDSIFFGDFESLNTVAYDHSNKDLKNFIRSNPDTESSNSIISDADEQLLLHIPLTNLAKIYSILIKFKSPVKKGEVEIDEVQKPKNIKVWNNRTSIISFEDVDSFISQHEEELLDNGWDSNGWYEIKLKYVRFQKVSSIDLFIDGDDDELHTLVDKVVLIGVDGESKNQGKIEKLGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXV4</accession>
    <name>A0A1E3NXV4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">WICANDRAFT_33513</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP = N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13501"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hydrogencarbonate + acetyl-CoA + ATP = malonyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11308"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037228.1">
        <property type="nucleotide sequence ID" value="XM_019182080.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3NXV4"/>
    <dbReference type="GeneID" id="30199326"/>
    <dbReference type="OrthoDB" id="14612at2759"/>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004075">
        <property type="term" value="F:biotin carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.460.10:FF:000001">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000005">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000010">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.460.10">
        <property type="entry name" value="Biotin dependent carboxylase carboxyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1770.10">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049076">
        <property type="entry name" value="ACCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049074">
        <property type="entry name" value="ACCA_BT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013537">
        <property type="entry name" value="AcCoA_COase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728:SF3">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08326">
        <property type="entry name" value="ACC_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21385">
        <property type="entry name" value="ACCA_BT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0092">Biotin</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="19">
        <location>
            <begin position="58"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="18">
        <location>
            <begin position="216"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="17">
        <location>
            <begin position="694"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="20">
        <location>
            <begin position="1496"/>
            <end position="1832"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="21">
        <location>
            <begin position="1837"/>
            <end position="2153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1204"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="1242"/>
            <end position="1269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="ODQ58021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="2245" mass="249631" checksum="9EF81F82E8F38305" modified="2017-01-18" version="1">MSEENLSEVAAPQTTTNIPDFSALHKSIPQHFIGLNTLEVAEEGPVKDFVREHGGHTVISKVLIANNGIAAVKEIRSVRKWSYETFGNERAIQFVAMATPEDLEANSEYIRMADQYVEVPGGTNNNNYANVDLIVEIAERTEVDAVWAGWGHASENPLLPEQLAASKRKILFIGPPGSAMRSLGDKISSTIVAQHADVPCIPWSGTGVKDVELDPKTNLVSVNDTIYAKGCVNSPQEGLEAARKIGFPVMIKASEGGGGKGIRKVEDEKDFVSLYHQAANEIPGSPIFIMKLAGNARHLEVQLLADQYGKNISLFGRDCSVQRRHQKIIEEAPVTIARNDTFGEMEKAAVRLGKLVGYVSAGTVEYLYSHSDDKFYFLELNPRLQVEHPTTEMVTGVNLPSAQLQIAMGIPMHRIRDIRLYYGVDPHTSTDIDFNFENEGSLTSQRRPVPKGHCTACRITSEDPGEGFKPSGGSLHDLNFRSSSNVWGYFSVSNQGGIHSFSDSQFGHIFAFGENRQASRKHMVVALKELSIRGDFKTTVEYLIKLLETSDFEDNTITTGWLDELISKKLTAERPDPNVAVLCGAVTKAHIISEEARQQYISSLEKGQVSSKDLLKTIYPIDFIYEGYKYKFTAAKSANETYTLFINGSKVEVGAKPLSDGSLLVALGGKSHTVYWKEQVGATRLSVDSKTCLLEVENDPTQLRTPSPGKLVKYLVENGEHVNAGQPYAEVEVMKMCMPLIAQENGIVQLVKQPGSNVQAGDILAILALDDPSKVKHALPFEGIMPQFGAPTVQGTKPAYKFKSLVSTLQHTLGGYDNQVVMNATLQNLIEILRDPSLPYSEWNQQVSALHSRLPQKLDSDLSSLISRSEQRSAPFPARQLTKLLEKAATAPEVDGLFGQVIAPLLDITQRFSNGLEAHEHSVFASLLNEYYNVESLFTGPNNREEDVILKLRDENKNDLSKVVATVLSHSRVSSKNNLILAILKHYQPLCQQNSEVADALRSSLKQIVELESRATAKVSLKAREILIQCALPSIKERSDQLEHILKSSVTQTRYGEIGASHREPQLEVLKDVIDSKYVVFDVLTQFLVHPDPFVASAAAEVYIRRAYRAYNVIGSLVYHTTNDLPIVEWKFQLPSLSTAAYNAVPQSKNLLGKSPMGRAVSVSDLTFISTDDSQPLRTGILVPANHLDDVEDALVRGLEKIPHRHSSVSESRGPAPDRSNPQTNAVLSNIASVVVANADGFESEAEILAKLNEILAETKEELVATSIRRITFIFGSTDGSYPKYYTFRGPNYEEDETIRHIEPALAFQLELGRLSNFHIKPVFTDNRNLHVYEAVGKNSAVDKRFFTRGIIRTGRIRDDITIQEYLTSEANRLMSDILDALEVIDTSNSDLNHIFINFSAVFNVLPEDVEAAFGGFLERFGRRLWRLRVAAAEIRIMCNDPKTGVPFPLRAMINNVSGYVIKSELYIEVKNSQGDWVFKSIGTPGSMHLRPISTPYPAKEWLQPKRYKAHLMGTTYVYDFPELFRQSTLAQWKKYAPDAKVPDDFFNSVELILDDSDDVTEIQREPGANNIGMVAFKVIVKTPEYPRGRQIIIVANDITYKIGSFGPQEDYFFNKITELARKLGIPRIYLSANSGARIGIAEELVPLYQVAWNEPGNPEKGFQYLYLTQGGLDELAKNENSKSVVTERIVEEGEVRHVIKAIIGAEDGLGVECLRGSGLIAGATSKAYKDIFTITLVTCRSVGIGAYLVRLGQRAIQVEGQPIILTGAPAINKLLGREVYSSNLQLGGTQIMYRNGVSHLTASDDLAGVEQIMNWLSYVPAKRNMPVPILESQEDSWDRDVDYAPKRGEPYDVRWMIAGRQGEDGSFESGLFDKNSFQETLSGWAKGVVVGRARLGGIPFGVIGVETQTVENLIPADPANPDSTEVLVQEAGQVWYPNSAFKTAQAINDFNNGEQLPLMILANWRGFSGGQRDMYNEVLKYGSFIVDALVDFKQPIITYIPPTGELRGGSWVVVDPTINADMMEMYADVESRAGVLEPEGMVGIKYRREKLLATMARLDKTYGDLKRQLGDSSLSAEEHSAISAKLAARERALLPIYQQISVQFADLHDRSGRMVAKETIRKELEWVNARRFFFWRIRRRLNEEYLIKRIGEELKHATRIEKVARLRSWYPSTVDFEDDRAVATWIEENHEQLDLSVKQLKDEAFAQNIAQLIRTNHDSAISGLAEVIGLLSTEDKEKILKNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXX4</accession>
    <name>A0A1E3NXX4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial import inner membrane translocase subunit TIM16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Mitochondrial import inner membrane translocase subunit tim16</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 13">Presequence translocated-associated motor subunit PAM16</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_85160</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, it is required to regulate activity of mtHSP70 (SSC1) via its interaction with PAM18/TIM14. May act by positioning PAM18/TIM14 in juxtaposition to mtHSP70 at the translocon to maximize ATPase stimulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM16/PAM16 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037223.1">
        <property type="nucleotide sequence ID" value="XM_019185850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXX4"/>
    <dbReference type="STRING" id="683960.A0A1E3NXX4"/>
    <dbReference type="GeneID" id="30203096"/>
    <dbReference type="OrthoDB" id="10262892at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000006">
        <property type="entry name" value="Import inner membrane translocase subunit TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005341">
        <property type="entry name" value="Tim16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388">
        <property type="entry name" value="MITOCHONDRIA ASSOCIATED GRANULOCYTE MACROPHAGE CSF SIGNALING MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03656">
        <property type="entry name" value="Pam16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="108"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ58016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="131" mass="14573" checksum="CEC1AD9D652247B5" modified="2017-01-18" version="1">MAHRLLVQVIFTGAQVFGRAFTEAYRQAAQQTAKQSASSAGKAAQNFGITLDESAKILDVDLKNVSMDKVDERYNYLFDVNSKEKANSFYLQSKIYWAAERLKSELKEKDAAAKGEKSEAKTEGGEQQTKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY56</accession>
    <name>A0A1E3NY56_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FMP27 GFWDK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_58053</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036839.1">
        <property type="nucleotide sequence ID" value="XM_019182815.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3NY56"/>
    <dbReference type="GeneID" id="30200061"/>
    <dbReference type="OrthoDB" id="1562405at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019441">
        <property type="entry name" value="FMP27/BLTP2/Hobbit_GFWDK_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019415">
        <property type="entry name" value="FMP27_SW_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019449">
        <property type="entry name" value="FMP27_WPPW_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045167">
        <property type="entry name" value="Hobbit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678">
        <property type="entry name" value="ANTIGEN MLAA-22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678:SF15">
        <property type="entry name" value="PROTEIN FMP27, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10344">
        <property type="entry name" value="Hobbit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01214">
        <property type="entry name" value="Fmp27_GFWDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01215">
        <property type="entry name" value="Fmp27_SW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01216">
        <property type="entry name" value="Fmp27_WPPW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMP27 SW motif-containing RBG unit" evidence="4">
        <location>
            <begin position="664"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27/BLTP2/Hobbit GFWDK motif-containing RBG unit" evidence="3">
        <location>
            <begin position="785"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27 WPPW motif-containing RBG unit" evidence="5">
        <location>
            <begin position="1197"/>
            <end position="1672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="597"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2008"/>
            <end position="2030"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1370"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="2020"/>
            <end position="2030"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ57632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2146" mass="247373" checksum="1C6D62ECF3DC8CCE" modified="2017-01-18" version="1">MTSFPKLWPKVELKLSIENPIFLVKSPLLQQFYKILVLKFSLISVEFKTTRTLEDDQMFYSTSQVVNLANTKLKYIDTHDELDETALFWDDIDLKQTVDLVPQLQVSTFLISQSSILDLSNLKVLHGINSLIGELYQMTKPLFDQYKHDLVACGCIEKEQQKVQTLDTSSSDGEICDENTVTNDSLADLIPQWISQIVYVSNNTSIILGSRSLLIPKDLVKNIDPLSKNDLINGDLRKIAVTIEKWSISLLSQAGKIELENTTSSNSSLEDDDKGLLDSFSDEVSVKEGWKLKFESTKLLGKVVSESSKKHALADKVFLKIPICSILVGPMVEDVEQLSIDFNLEKIDIFYSVMTHFIIISSLHLLRNTIFEFLQYSEKSDVPKTPSSPEKKAGIEELLDRVHLNFNIDYLDLIFVAPDRMKLKCEVNRASAIVLPKSPILLNSHYMRLCAESPTTPGYWSRILTAVNGSTQVNIQDILANNDENTWFNLANESCHLTIPHQFIIYKVFENISVMAKTLRQLHHSLKYNTDEIVINPKAKPAHKIPRTNLKSNRLLFSMDDDPFEAELNMIFQIGLVEQKMRLEKTKMFDARISEELQNQRQKAPKSATFPTSNIGHDVNSDLPNISNKRKFLKGKPARFSSNSSAKLAPDFNKIFDVRQNALEKLHKMREGFSKSWIARIRAYKKNQEREFQQNFKYLWGNLNMDHLPQDFNKKVLDFVTSPSLFILALEDVDLNIAKPSFGIEGIPDFIYDVGKGKPKDSQYSTLIPLFFDLKLGELRCHLRDYPLPFIYMPRMTKAQDKNLSAVRIHGDVVIGENTIKSHKEVREVYVPLVPGCREFDEDNLYSIEVPKTLTSIKFYTKLDWDLNSADTTKVTWGTSYQPCIQQIMLNLDNFTKPPIDPSEKVGFWDKLRANFHARFSFHWKQNGGFDVIFKGSKNPYDICGIASGFLLGFKDGVTLSVNEKDDPREFLVANSNIVMFAVPNHLAEPLLVWCRETEKSIFLANQSTNFQGSTYGYYFNVDEIPDAAQVDIMAEHYLEKVAIKLSGGVQFNLAFFFERKTDDGNERTSDFISHDEVLLCNPKYVEDRDNYDAYYQFRSHYIHMGLTLVSKSEDAYNTIQLTPAMFEHFFKWWHMFSNTFPVRHGKLFGPEKATKKFARHLYTIKYQAIADPLFITHVYHDHDLKRQDQDKLSSVGIKSRASNFVLDLHQRKELTYEHNPKLDVTKKIMRMGFNIGQVSMTDLDMRTMNGLFELDKDLNMARKRATGEKTSHNHHVEVFDGDKSWFDIRDFTEIGTSTLDGYKSSVQIHPLMFTPSFMYFKRNDHGDKFQINIDTGERIRPFGKEKFHNCLFSGHDSNKVQQDSFTTRIDELTSKRKEKLAQVEQMETTFPQTPTAHRDISQLQLEIRKLNHAIQFVRDLAMANEARSPTEVIENSSFDFNINDDFEQSPFRNKFIIHNMLLKWNDKNRDVVYKYVYLFEMTSQFSKFMQHKSLGQIEDIIEAQARRAEHSRFSRQATHVTQATYESKGLAETETHPYDKSVNETRASEKLKNFEDELYEIPIDFSYITQENYLLKLISPQIQLQSENSKDSAVMIVAPNIELKVLAFDVNDTDNEYNENIVEQRFGAVLTDASAFVFYDDDIQSRHNVIFSNSNYGSTTSWPPWVGIELCYDGAFLKNHMLMEKTSVVLRYDKPDESYFVNDKENSINKMSCDLSQAVFKCDARQYYSLYTMIMSLLIYNEPRNVKLKEKESKMLLRLDVNDISSLKDRILKLQESIRQMKKIENNLTGRRSILDDVERNDLLVVSTKKEEAMEEVYLLMHVALLGGRQAKASDDYLEWDIRADDIKLHMLDDERLPFLDVLLTNSHFKRVEGSDRSNRNIVVIETVEVLNLEKDTQLPVLFAPYENHKRRKDSNKKSKGHYNVKQQPPPMIHVIWKMDNPVGGIRVIRKFEVGLEPLQLNVDQFTGEKIMKYAFPDSIKSSINGNQPSAKKSIFSFKSKDGSVNGNGSASHIIHGLDEEDDDQDDDEEIEEMIRRASNYLSIVSFKFKSCVISVTFRGHGSKRLVNVTDFALTLPEMTFTNQTWTLLDLTMEIKKFAIKALLSHTGSILGNKMVNHGRKRFDRPNRDNIRRSTSRISSFRSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY80</accession>
    <name>A0A1E3NY80_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_19313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037333.1">
        <property type="nucleotide sequence ID" value="XM_019180824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY80"/>
    <dbReference type="GeneID" id="30198070"/>
    <dbReference type="OrthoDB" id="3980689at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020999">
        <property type="entry name" value="Chitin_synth_reg_RCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28187">
        <property type="entry name" value="PROTEIN RCR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28187:SF1">
        <property type="entry name" value="PROTEIN RCR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12273">
        <property type="entry name" value="RCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="98" mass="11101" checksum="38F6CEBAA9D1FF72" modified="2017-01-18" version="1" fragment="single">SSSSWVWGRWILFVLFIVAILCVVLAAIRINKRRGQEGREPIRGTAWITPPSYVQSQHQTNTQQPFVPPYSEQANDNDAGYYDNQGVFHPNLKAQEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYC9</accession>
    <name>A0A1E3NYC9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_24037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037207.1">
        <property type="nucleotide sequence ID" value="XM_019181013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYC9"/>
    <dbReference type="STRING" id="683960.A0A1E3NYC9"/>
    <dbReference type="GeneID" id="30198259"/>
    <dbReference type="OrthoDB" id="48317at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08249">
        <property type="entry name" value="enoyl_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047122">
        <property type="entry name" value="Trans-enoyl_RdTase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348">
        <property type="entry name" value="HYPOTHETICAL OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348:SF2">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C2E1P3.01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="11"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="329" mass="37256" checksum="2F8172E46812BF9A" modified="2017-01-18" version="1" fragment="single">SDSQTAVVINSFDEDFTIDYNYPIPELGEYEILIENKYIGLNPVDWKGKKYKFGIYSFPWIQGRESSGIVQKIGSKVSNLTKGDKVFIASTSYRDLRTSTFQNFTIFDSRLVWKLPENISLKESCTIGVGLITAGSVLEELNVNLFDHEEFDKAKLLHQRENLLIWGGSSGVGSYIIQLAKVAGFKKIITVATLKHTDFLHKIGATHVLDRFKSKEDLIKDLKEIVPEGLQIGIDVVGKETTDTVIEFLNPSKDLDSKLLENIEVKQVLIKKFHENLKFGENLVAKTWELLSNEQIKPQKNLKIYKGFEGILNGLKDLEKFGASNEKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYF3</accession>
    <name>A0A1E3NYF3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_97145</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036939.1">
        <property type="nucleotide sequence ID" value="XM_019186568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYF3"/>
    <dbReference type="GeneID" id="30203814"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="152" mass="17774" checksum="98844BC72428D7F9" modified="2017-01-18" version="1" fragment="single">MVNIKSIHPVTTHIPKSFVQKKTEDMERYISEVIKNIPDRIVIRCISKSLKNIEGGYKIGRQTNKADIEELFRRQIIGPIMKFFEMIWDYDSTSNENALRCCKHFSFARMANPEPSYGADRRLRNNVELITPDFSFINTTPSTYSRGYNLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYG0</accession>
    <name>A0A1E3NYG0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_70122</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037365.1">
        <property type="nucleotide sequence ID" value="XM_019184193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYG0"/>
    <dbReference type="GeneID" id="30201439"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="361" mass="41528" checksum="CCE05FFEC9277C85" modified="2017-01-18" version="1">MSIPLTIEDLPAHIFETVVLFGVSPVEMMPVNRHFLNIFGPILYRSVHCIYSADFAWTKKVAPPCEDEVRDGYHKRRGTMMEMMPVSETKVTLISKCPQLIYFVFDVLIEGHLMKKFIKNLSFDVLIVEDDECLDMNSKLLKDTYGIVDIDDVSNGYRSTLITALQDYVPFRKLIERDFSIFSTRSSSLEAKRASPNEVHFHNEAHKQRSIYQSNQLTLPMQIVYNYLTGKRFTRDPFQEYNSIFKDVQIRHYDFTTLFSTNGLTLLPSYTKRMRKMSSLIDLQLSPEMITNTMARSQVYEILKSIIIGLYSLSPSCKGEFCGILVKSRDGRSSGRTSRNSPDIITSQRAKLVGFKVGPQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYH9</accession>
    <name>A0A1E3NYH9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_64300</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037362.1">
        <property type="nucleotide sequence ID" value="XM_019183705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYH9"/>
    <dbReference type="STRING" id="683960.A0A1E3NYH9"/>
    <dbReference type="GeneID" id="30200951"/>
    <dbReference type="OrthoDB" id="2985014at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17327">
        <property type="entry name" value="MFS_FEN2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000068">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000018">
        <property type="entry name" value="MFS transporter permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF24">
        <property type="entry name" value="NICOTINIC ACID PLASMA MEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="290"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="354"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="414"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="57"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="499" mass="55221" checksum="F559EE853C4C9FFE" modified="2017-01-18" version="1">MSSQEKKPVVQEEITETQLESQTSVLDFKSDSLIDEYEGQKNPQLEKKMLRKMDMVILPIIGWVYLIAYLDRSNIGNAASAGLIEDLNLSPSNYSAAISLFYVTYILVETPCTILFKKVKPAVMLATLTFSWSLVGIFSGFVKNFSGIVVIRLLLGLIEGGLFPCLTLYLATVYQRNELAYRVSYLFSAAALAGAIGGLLAYGLLQMDGLHGLKGWQWLFIIEGCISITGAILVLGALPNNMEKAWFLNDEERAYYSMRKRVHEHNLLKDQEKEKEGSQDVKAAFKDPKVYLSAISQFCAEVVFYGFSTFLPQIVKAMGYTTLKTQLLTIPIYVVGSISFIMIATVSDYFKRRLPFLILAACFPIIGYIMLLASNSNSVRMCGVYILTVGAFAGPGLNLSWLNNNTAGHLKRATSVGIQLSLANVAGVVIGQIYRSNDAPEYKLGHGFSLGCISFAIISYAAQGLYLRSKNIEKRDRLLNGTSKPDARGDNSDSYTYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYL0</accession>
    <name>A0A1E3NYL0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">1,4-alpha-glucan-branching enzyme</fullName>
            <ecNumber evidence="4">2.4.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glycogen-branching enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_106540</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Glycogen-branching enzyme participates in the glycogen biosynthetic process along with glycogenin and glycogen synthase. Generates alpha-1,6-glucosidic branches from alpha-1,4-linked glucose chains, to increase solubility of the glycogen polymer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.</text>
            <dbReference type="EC" id="2.4.1.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.18" evidence="4"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037361.1">
        <property type="nucleotide sequence ID" value="XM_019180477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYL0"/>
    <dbReference type="STRING" id="683960.A0A1E3NYL0"/>
    <dbReference type="GeneID" id="30197723"/>
    <dbReference type="OrthoDB" id="196493at2759"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11321">
        <property type="entry name" value="AmyAc_bac_euk_BE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="E_set_GBE_euk_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="1,4-alpha-glucan branching enzyme"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037439">
        <property type="entry name" value="Branching_enzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF3">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000463">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="12">
        <location>
            <begin position="158"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000463-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ58154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="687" mass="78319" checksum="77265A2BEB8A1648" modified="2017-01-18" version="1">MSNIPPSVKGAVDADPWLTPFANELAKRRALADDWLTKFDQNEGGLTKFADSYKKYGFQIDPKDGSINYKEWAPNAVEAYLVGDFNNWDFTHKLTKDSFGNFDIHLSKGSIPHDSRVKIVLILPSGEKLYRLPAWITRATQPPKESKEVAFEARFWNPSTPYVFQNKRPIPSQSLRIYEAHIGISTPDPKIGSYKEFTQNVLPRISKLGYNTVQLMAIMEHAYYASFGYQVTNFFAISSRFGTPEDLKELIDTAHGLGITVLLDVVHSHASKNVDDGLNNFDGTDYQYFHSGGKGSHPLWDSRLFNYGKYEVLRFLLSNLKFYLDVYQFDGFRFDGVTSMLYLHHGVGAGGAFSGDYNEYLHENSAVDYEAITYLMLANDLINLVAKENSTNFVSIAEDVSGMPTLGTPRIDGGVGFDYRLAMALPDMWIKIIKEQKDEDWDLNKIVHTLTNRRYTEKAIGYCESHDQALVGDKTLAFWLMDAEMYTNMSVMSPLTPVIDRGLALHKIIRLLTHSLGGEGYLNFEGNEFGHPEWLDFPNVNNGDSYHYARRQFNLVDDDLLRYKFLYKFDSAMQHLEAKYGWLHKKGDTFISLKNEQDKVLVYEKAGLLFIFNLNPTQSFTDYRVGIETGGVYKIVLNSDIGEFGGHDRVDNDTRFFTTDFPWNNRKNFLQVYIPSRTVLVLALESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYL5</accession>
    <name>A0A1E3NYL5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_85287</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037402.1">
        <property type="nucleotide sequence ID" value="XM_019185869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYL5"/>
    <dbReference type="GeneID" id="30203115"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="80" mass="9144" checksum="D368346652B881F7" modified="2017-01-18" version="1">MNAFKVRENIADLQENIAKTTLKDVQLPKFVQQLDEINKFYPFIMSSGLVIKSSTSVLYLSLSLMMLNLDYFVSSTFVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYW0</accession>
    <name>A0A1E3NYW0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alcohol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_70287</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037566.1">
        <property type="nucleotide sequence ID" value="XM_019184199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYW0"/>
    <dbReference type="STRING" id="683960.A0A1E3NYW0"/>
    <dbReference type="GeneID" id="30201445"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000947">
        <property type="term" value="P:amino acid catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08297">
        <property type="entry name" value="CAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000002">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="10">
        <location>
            <begin position="39"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="372" mass="39789" checksum="6B032AB0F02A4A79" modified="2017-01-18" version="1">MSLRTIMGTITPKLSTATRGLATQAIPKTQKAVIFYEHGGPLHYQDVPVPTPGPNDILVNIKYTGVCNSDYHAWKGDLDKKPMLPLIVGHEGAGVVVAKGDNVKNFQIGDYAGIKWLNSTCNSCDFCEESNEANCPEQKNSGYSVQGTFQQYCIGDATQAAKIPQGTDLSKVAPILCAGLTAYNALKHANIKTGQWVTIIGAGGGLGSLAVQYAQVMGFKILGIDGPNKEEFVLSKGADAFIDFSKVTDTGEAVREITGKDGPHAVINFSNSLKAYNDSVEYVRATGTVVLVGFPGDNAALSSNIYSHINRAINIKGSVVGNRADTREAIDFFVRGKVEIPVKIAGLSEASNVFKKMEEGQILGRYVLDTSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZE5</accession>
    <name>A0A1E3NZE5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_63098</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037793.1">
        <property type="nucleotide sequence ID" value="XM_019183489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZE5"/>
    <dbReference type="STRING" id="683960.A0A1E3NZE5"/>
    <dbReference type="GeneID" id="30200735"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000702">
        <property type="entry name" value="ABC transporter (Adp1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF2">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="715"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="748"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="791"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="830"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="858"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="915"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="944"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="43"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="328"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ58586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="967" mass="108734" checksum="6998C24C4BE6B35C" modified="2017-01-18" version="1">MSSNTEDDKCPPCFNCMLPAFECKQFSKCNEFNGQCECIDGFGGQDCSEPLCGSLTKDSNRPIRDGDSCDCDDGWSGINCNVCKEDSVCDKFMPEGLSGTCYKNGMLVNKFHQMCDVTNKKIVEILNGKKPQVTFSCDKKESTCNFQFWIAEKESFYCDLSTCDYQFDLKSNASHYKCKDVSCKCVPGRMLCGESGSIDISDFLTETIKGPGDFSCDLDKRNCKFSEPSMDDLILSVFGDSYITLHCDSGECIHYSEIPGYDIPKKPKFTLKSLFNVVAVIIGVVAVLVGAVLTIRKSPLFSGAQISLDDYSDEDDQDSLLNYTPVTLTFEDVSYSVGSKAILNKVTGIVKPGEMLAIMGGSGAGKTTLLDILAQKNKNGVVTGDIKVNGNSVSRHDLRKIIGFVDQEDYLLPTLTVYETVLNSALLRLPRNMSFKAKERKVYEVLRELRILNIKDRTIGSDFERGISGGEKRRVSIACELVTSPSILFLDEPTSGLDANNASNVIECLVRLSRHYHRTLVFTIHQPRSNIVSLFDKLVLLANGELIYSGEMIQCNDFFYNNGYKCPKGYNIADYLIDITFSEKHSIGRPNQFQVAGIDEENADYGDDQDIHIPRRSSSTSDTQREWEHFALHRDDAGFNKAVKNHPEEVRVDTKIYDMFQNSQNFEMLKDEIENAKVNSSEIVFKGNYQKASFLNQFIILSSRTFKNLYRNPKLLLGSYILSVFMGFFCGFLYYNVDNDISGFQNRLGLFFFFLSFFGFSTLTGLHSFSIERIIFLKERSNNYYHPLSYYISKIICDVLPLRIFPPILLTSIAYPLIGLNLENNGFIKCLLILVLFNLTVSIEILIIGILVKDSSNATMIGVLTLLFSLLFSGLFINRDSLKFGFLQYLSMFHYGYEALAVNEVKTLILREKKYGLSIEIPGATILSTFGFNVGGLVKDVISLGLWNLLFLVLGYVSLYFYVVEQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZW4</accession>
    <name>A0A1E3NZW4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Elongation factor 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Eukaryotic elongation factor 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_34195</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037364.1">
        <property type="nucleotide sequence ID" value="XM_019182163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZW4"/>
    <dbReference type="STRING" id="683960.A0A1E3NZW4"/>
    <dbReference type="GeneID" id="30199409"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="UniPathway" id="UPA00345"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002182">
        <property type="term" value="P:cytoplasmic translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18626">
        <property type="entry name" value="CD_eEF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1390.20:FF:000001">
        <property type="entry name" value="Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000076">
        <property type="entry name" value="Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.990:FF:000001">
        <property type="entry name" value="Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000193">
        <property type="entry name" value="Probable Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015688">
        <property type="entry name" value="eEF3_ABC2_chromodomain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047038">
        <property type="entry name" value="eEF3_chromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040533">
        <property type="entry name" value="EF3_4HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047036">
        <property type="entry name" value="EF3_4HB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF5">
        <property type="entry name" value="ELONGATION FACTOR 3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17947">
        <property type="entry name" value="4HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24984">
        <property type="entry name" value="HEAT_EF3_GNC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <feature type="repeat" description="HEAT" evidence="15">
        <location>
            <begin position="91"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="15">
        <location>
            <begin position="171"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="17">
        <location>
            <begin position="428"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="17">
        <location>
            <begin position="670"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="978"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="990"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1023"/>
            <end position="1034"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ58157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1047" mass="116058" checksum="2C96DC00D0F9EDA9" modified="2017-01-18" version="1">MSDSKQSVEVLNELFTKLSVATPEEREAAATNVASFLNGSIIEHDVPEQFFAELKKSVENKKSAANAIEAVAHIASENNLSPSVEPFVVDLVPAICAKSGDKDNTIKDIAAKALVAISKAVNPHSIKALLPHLTKALAETNKWTEKIAILSAITAVVESAEEQVALRMPELIPVLSEAMWDTKPEVKKAATATITTSTNTVHNKDIEKFIPKLIECIGKPSEVPETVHILGATTFVSEVTTATLSIMVPLLSRGLAERDTSIKRKAAVIVDNMCKLVDDPQVVAPFLDKMLPALKSNYQTIADPEAREVTLRALNTLRRVGAIGADDVIPEVSHAGATEVTKKELTNIFAESKTEVAPRFQLVLEYASAIAGDLIDERIIDQQAWFEHLTPYLTVFLHEKQAKADIDEWRKRAVDNIPQPPQFEDEDDEGEDLCNCEFSLAYGAKILLNKTQFRLKRGRRYGLCGPNGAGKSTLMRAIANGQVEGFPTQEECRTVYVEHDIDGTEADTSVLDFVFGDGALGTKEAITDKLREFGFSDEMIAMPIQSLSGGWKMKLALARAVLKNADILLLDEPTNHLDTVNVAWLVNYLNTCGITSIIVSHDSGFLDNVVQYIIHYEGFKLRKYKGNLSEFVKRVPSAKSYYELGASDLEFRFPEPGFLEGVKTKQKAIVKVSNMTFQYPGTSKPQIQDINFQCSLSSRIAVIGPNGAGKSTLINVLTGELLPTIGDVYVHENARIAYIKQHAFAHIDSHLDKTPSEYIQWRFQTGEDRETMDRASRQINENDEEGMKKIFKIEGTPRRIHEIHARRKFKNSYEYECSFLLGENVGMKNERWVPMMSVDNAWLPRGELVETHSKLVAEVDMKEALASGQFRALTRKEIESHCAMLGLDAELVSHSRIRGLSGGQKVKLVLAACTWQRPHLIVLDEPTNYLDRDSLGALSKALKAFEGGIIIITHSAEFTKDLTEEVWAVMDGRMTPSGHNWVSGQGAGPRLEKKDDEGDKFDAMGNKIASAKKKTKLSSAELRKKKKERMKKKKELGDAYVSSDDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0G7</accession>
    <name>A0A1E3P0G7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pre-mRNA-splicing factor CWC22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_57269</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC22 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037619.1">
        <property type="nucleotide sequence ID" value="XM_019182797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0G7"/>
    <dbReference type="STRING" id="683960.A0A1E3P0G7"/>
    <dbReference type="GeneID" id="30200043"/>
    <dbReference type="OrthoDB" id="3938623at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000004">
        <property type="entry name" value="pre-mRNA-splicing factor CWC22 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050781">
        <property type="entry name" value="CWC22_splicing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034:SF3">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC22 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MI" evidence="8">
        <location>
            <begin position="376"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="574"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="574"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="591"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="611"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="728"/>
            <end position="740"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="767" mass="88516" checksum="79C54B9C0C55FC28" modified="2017-01-18" version="1">MSETQRFLDQVVKDLDKSAPSNGLSEPEESKRIDPLEEYKRLQELRSGGRYVPPAKLRALLDQINVSKGSKDYQKMEWEKLKKNINGLVNKANTGNIKVIVVALFNLNLHKGLGLLIRSVMKAQSLALTFTPVYAGLIAVLNSKLPQIGDLLISRLVIQFRKAFKRNNKALCISSTTFLAHLCNQQVCHEILALQILHLLLNNPTDDSVDIAVGLLKQIGQYLSEITPAATSDIFEKLRQILQEGVLEKRTQYMIEVLFHIRRDGYKEYPIIPEGLDLVEEDDQVTHKVGLDDKLKAQDGLNVFQFDEQYEEHEEDYAKIRIEILGEDEDDEEEEYEDDEYEEVAAENVSNDENQDEDNDVGKLEIKDMTETNLNNFRKNIYLILKGSMSADEAVHKLLKLQVKVEDQDKVVDMVVKACSQETTYSKYYGVSGEKLCRQGRTWSDAFKKIFTENYNTIHRFESNPLRNVSKFWGHMLSSDALDWEVLEVIHLTENDTTSAGRIFIKFIFQELVEELGIPGLKERLDEEYIQPFLHGIFPVENADDLRFSINYFTAIGLGVLTEVMRETLQDLTNKKREEEESRGRSKSRSRSSSYSRSYSRSRSYSDSRSRSYSRSRSYTRSPSYSRSRSYSRSRSYSGSRSRSPRRYSRSRSYSRSPPRRNYSRSPSYSRSPPRRVYSRSRSYSRSPPRRYSRTPSRTPSRTPPRKSSRTPSRSPPKRFPRSPLRSQGSSYRDSYNNNGRAGFNTRGRGNYRGRGRGRGDYTSYRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0I6</accession>
    <name>A0A1E3P0I6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Vacuolar ATPase assembly protein VMA22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_63402</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038102.1">
        <property type="nucleotide sequence ID" value="XM_019183539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0I6"/>
    <dbReference type="STRING" id="683960.A0A1E3P0I6"/>
    <dbReference type="GeneID" id="30200785"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990871">
        <property type="term" value="C:Vma12-Vma22 assembly complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040357">
        <property type="entry name" value="Vma22/CCDC115"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31996">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31996:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21730">
        <property type="entry name" value="Vma22_CCDC115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00093634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="201" mass="23308" checksum="307C6D7AC5D62F5B" modified="2017-01-18" version="1">MPGKDPEITEKLESLTLSDDLLPNYTTVLETTSEKDQLLIKYLSLLDQYNQAVEQLNHEFKEGYINLSRANYANTSVVKPYGRDCWDDRVKAIKKVSIGDKIELIDVEEKVDKVKDDDEKDQSIRNRSKKVEETSTKKKKKYNPINMFGVLVPLQLRQSQTHFNKSVIQMISIVNLRNELISMEDELKIKNIDLEKKVDCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0K0</accession>
    <name>A0A1E3P0K0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_93510</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038055.1">
        <property type="nucleotide sequence ID" value="XM_019186417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0K0"/>
    <dbReference type="STRING" id="683960.A0A1E3P0K0"/>
    <dbReference type="GeneID" id="30203663"/>
    <dbReference type="OrthoDB" id="29851at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101031">
        <property type="term" value="C:protein folding chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006452">
        <property type="term" value="P:translational frameshifting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000010">
        <property type="entry name" value="heat shock 70 kDa protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="463"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="463"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="473"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="545" mass="58706" checksum="29DB73DBECFE27C2" modified="2017-01-18" version="1">MSTVIGISFGNTTSSIAAPAQDGKGVEVIANPDGERAIPSSLAYVGDDEYHGGQALAQLIRNPRNTIINFRDYLGQAYSKVDPTVTERAAHAADLDGKIGFEITKTSESGEEKVEKITAEQATARHLGKLKVAAEDYIGKKVEGAVVAVPTDFTEEQKAALVRAGDAADLKVLQIINEPSAALLAHASTEDRLEQDKTYVVADFGGIRSDAAVISVRGGILTILATSHDYELGGDKLDAALAEFIAKEFEKKYKVDPRKTARSLAKLKDAAILARKTLSNVQSSTISIESLAEGIDYHSTINRLRFELVARNVLSQFTGFVESVVKKANLDVLDIDEVLLAGGVSNTPKLASNVELLFPESTIVVAPSLDSKVPYPEELVARGAALQASLLENFDENEIKESLSPVIINTQHLSKAIGIVNGAGEFVPILKSEATYPIRRSIQLDAPKGDVLITVVEGEHHVKETVLDPPAKDEDDEDDYSDDEPEVVREKHYLPGTKLAELAVRDVHEGSKVEITININKEGTLQVSAREARQGSVAFKGELSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0Q3</accession>
    <name>A0A1E3P0Q3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_34447</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-5/BimC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037704.1">
        <property type="nucleotide sequence ID" value="XM_019182187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0Q3"/>
    <dbReference type="STRING" id="683960.A0A1E3P0Q3"/>
    <dbReference type="GeneID" id="30199433"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000235">
        <property type="term" value="C:astral microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005828">
        <property type="term" value="C:kinetochore microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098653">
        <property type="term" value="P:centromere clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000073">
        <property type="term" value="P:initial mitotic spindle pole body separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045144">
        <property type="term" value="P:meiotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061805">
        <property type="term" value="P:mitotic spindle elongation (spindle phase three)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033047">
        <property type="term" value="P:regulation of mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000019">
        <property type="entry name" value="Kinesin-like protein KIN-5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047149">
        <property type="entry name" value="KIF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970:SF12">
        <property type="entry name" value="KINESIN FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 9 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="7"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="898"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="409"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="98"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ58497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="931" mass="105522" checksum="09F3F54897A1D3E7" modified="2017-01-18" version="1">MSSENENIQVVVRCRGRNAKETSIKSTVVVNVEDEEGYEVALNPSNDTGVSAQLNSKTYAFDKVFGPMSDQQVIFDKVATPMFNEFLKGYNCTMLVYGMTSTGKTYTMSGDDKLVDGELSDKAGVIPRILFKLFGVLNESKEQLNEDYIVKCSFIEIYNEELKDLLTDETNKKLRIFDQKAVPGNASPIPGQSNNKSTIHIQNLEEVILRNANHGIKTLQKGLKIRQVAATKMNDVSSRSHTIFTLSLFKKIGESGDLFRISKMNLVDLAGSENISRSGALNQRAKEAGSINQSLLTLGRVINLLVEKNQQHIPYRESKLTRLLQDSLGGRTKTCLIATISPAKINYDETLSTLEYANKAKSIENKPQLGSSIGREILVKELSIELSKVKSDLTSTRLKDGIYMNNDNYEEFMNDIQEYKTEINETKRTSELLRKQNESLKKKTQLLEFQNNEYMTMINTLNATVESLHNKIDKQKQNEENLMMSSSKFKHIINSINSNLMIFKKYEGDAKSEINTINEVYIQKIVEKIKNTIEENSKSPNIDSIIPNIQHEFHQMIIDLQRATYDACKNVSESILSKFPDLFDDFEGNIEELRSLIKIFEVNVSEEFSKLSKQNNDLKSTLNESLFNNHDEFLNSVVNKASAQIEDDSNDLLNKFVELIRENNFKQQKALVDNIRSSAKHIVSLEKAQLQPAQTKWEHATCEIINKCDSQTNMLVQQHETKLHEISNSLKETNKFTTTSKAAIDNELTKINQLTSGFQNNSKVNLNLTEIKSKFEELETQKINFINSLNLTKDDTLKIRDHLIEKIVNNDEISNLEVKDVDKVLNELDTEQKEIKKALLPTGKTPLRQTLKIPKKLDERASEVLNRSPPKFQLIGQRFSGGSKDLVAVDNKENTVSNKRKFNSNGASELDSKKIPKLSGGGRIPLRRHKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P192</accession>
    <name>A0A1E3P192_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_93166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037894.1">
        <property type="nucleotide sequence ID" value="XM_019186405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P192"/>
    <dbReference type="GeneID" id="30203651"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="133" mass="16050" checksum="FD81B487EF51A211" modified="2017-01-18" version="1">MEMEYTGCESEVTQALYHCLIYPLTKLFNIVYNMTLKASYGIRLEDTMIPEFPRFERTKHGSTVFSDVKGKRGVHLVLEAGKPFLSQLFNSKERGFNRQYRDFYDCKERFAYRWCINAFYPFQSNRYRKWKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1B2</accession>
    <name>A0A1E3P1B2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alcohol acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_79254</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037914.1">
        <property type="nucleotide sequence ID" value="XM_019184864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1B2"/>
    <dbReference type="STRING" id="683960.A0A1E3P1B2"/>
    <dbReference type="GeneID" id="30202110"/>
    <dbReference type="OrthoDB" id="3979966at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052058">
        <property type="entry name" value="Alcohol_O-acetyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010828">
        <property type="entry name" value="Atf2/Sli1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037:SF1">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07247">
        <property type="entry name" value="AATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="481" mass="55265" checksum="AFD817FD1AA0249E" modified="2017-01-18" version="1">MEQPIFKYMVNQDEKSKKELELKIQNGHARPMGHVENLFGIYQREKIYKSFSIICKYNKVINDEALLFHALRPILVEYPLLSAAIVEQKIEDHVKPRPHDYTKVVDKVQLSDVMYELDEEIKALKGCELLEAMNSIVLPYGDDQLTWRLAIIDEFTLAYISNHVLADGITGKNLFQDLQIQFSKLYGSEIPPISPKSLLFNYNTDSTNLTHLPDPSDSVIDYAPPYSFIPEYLLNRFIISKFCPNSHASPGVSINYQTIHISASELSRIKQDLIEHEQDKKITLTPYIQAAWLNALYKTGVYNSKVTNFLLAVDARQYFPQENIDQYKYGLNTSATNKYFHNIKDFTWSWVGYLNDYIKWCVNTKRPLYLLGFLTLDKVVGNKNLDVEVRKTKHDKIRTNTLFSNLGVVESNRKEVVDIEDCIFTQHFNGSFYDFSINAITTRKGGLNFVISSTGNASFSKAKFNEAVLAFKENLIGECCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1C5</accession>
    <name>A0A1E3P1C5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="3">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_31623</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 14">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase delta/II small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="3"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037924.1">
        <property type="nucleotide sequence ID" value="XM_019181861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1C5"/>
    <dbReference type="STRING" id="683960.A0A1E3P1C5"/>
    <dbReference type="GeneID" id="30199107"/>
    <dbReference type="OrthoDB" id="3763at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016035">
        <property type="term" value="C:zeta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006278">
        <property type="term" value="P:RNA-templated DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07387">
        <property type="entry name" value="MPP_PolD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.430:FF:000002">
        <property type="entry name" value="DNA polymerase delta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040663">
        <property type="entry name" value="DNA_pol_D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024826">
        <property type="entry name" value="DNA_pol_delta/II_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041863">
        <property type="entry name" value="PolD2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416:SF0">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18018">
        <property type="entry name" value="DNA_pol_D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA polymerase delta subunit OB-fold" evidence="11">
        <location>
            <begin position="31"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="10">
        <location>
            <begin position="184"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ58717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="441" mass="49483" checksum="128B566716D9AEE2" modified="2017-01-18" version="1" fragment="single">QQSRSSIQVPSDYKSKDLFALDFNDRPYGKQFASIYYNRLSHLRPRILHNANKKWGKEVINGKKVVKKDKVLDIKSDEPSWVVGTVYCEMKYKPNILEEVSKGIQGANTSVKSYTDPDLDQIMLEDESGRVLLEGELLKNTILVTGTVVGVLGMESKPGVFQVVDLVYPFTSAQKPTRNASSKIALVSGLHFNGLDSVKYELLKDYLTGELGIESSRSISRLIIAGNSVLVSGEPEVDEKNKYGAKNKSNYNVDSVTQLDNFLVDLLQSIPVDIMPGESDPAETSLPQQPLHKAFFQKSKAYLNSSSFRTLTNPSWIQYDELRLLGTSGENINDIYKYVIPDSIKDTRIGMLEATIVWQNIIPTAPDTLWCYPYSDKDPFTLNETPHVYFVGNQPKYETKTLEIDHGMKVKLIAIPTFSETGQFVLLDTNTLETEIVSLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1E7</accession>
    <name>A0A1E3P1E7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_93268</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037944.1">
        <property type="nucleotide sequence ID" value="XM_019186411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1E7"/>
    <dbReference type="GeneID" id="30203657"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="485" mass="55711" checksum="A1B46EC90286C2A0" modified="2017-01-18" version="1">MSQQSKSREQRFKKYTNRHQVPVHPLESDTSTTIYHDALELQGDNSDTANSDTTITETSNHPTEFTSSNFETTPTTETEMPQSTTADALANLFQQVQSLTLQLSEVRESVPINEIQPDITSTLKRFFPSLIIEENKVLGTKLLSEISGSQIVVKENELIRQFDSNICQQLLNFQTRAGFLQLSFSQYGNFLYQFLDSYNKAVINKNTNIQDEERNYNYLQMLKTLLQDYDFHREEITRFQNLLQCEPVIGQTVSGYFETLFQMSRDVHSFSSYSVIQFRVIKILDLIIPNLNLHQFFIIQNRNEFYTLLSRYNLSQHKFTAELISQLNSSPQLVNTSTTAPPTSFPNSAITYAVNPSIKRFNKSNNNKSTEPSKRKFKNYFCKRCSCSRCEINKKLYDDYIKRKPASNAAVNVIYQDDLLDAEIELIPNDQLHFEEADDVLGIEEITPPSDAASGLDDETQEQQVLYEITANAGLTSNTNYRPIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P272</accession>
    <name>A0A1E3P272_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_63498</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038204.1">
        <property type="nucleotide sequence ID" value="XM_019183560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P272"/>
    <dbReference type="STRING" id="683960.A0A1E3P272"/>
    <dbReference type="GeneID" id="30200806"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000254">
        <property type="entry name" value="MAL31p Maltose permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF5">
        <property type="entry name" value="ALPHA-GLUCOSIDES PERMEASE MPH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="190"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="406"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="434"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="474"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="508"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="83"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="590" mass="65829" checksum="D06898D6D265EC0D" modified="2017-01-18" version="1">MNFTHDFVELDNKKDELITQITEEPIVNKQTSGSTSGGYTDNQIDEYLSRFLDISSNAQGNEHSEKQMSLKDGIKTFPKAAAWSFVLSSSIIMEGYDTNLLNSFYALDGFNRKYGQYYESIGKWQIPAKWQTSLSMAVFVGEIFGLFIAGIVADKIGYRKTLISALSVVTAFIFIVFFSINVQMLLAGEILLGVGWGMLQTLVVTYATDVCPLVLRVYLTTYVNACWVIGQLISSGILRGIVSSSDPHAYRIPFGIQWVWPIPISIGIYLAPESPWWLTRKGKAAEAKRNIKRLLTANSKTPDLDLLADAMFNKMQLTIKEEETMTAGVSYIDCFRGENFRRTRIAALIWLCQNITGSAFMGYSTYFYEQAGLPDTMSFTFSIIQYVLGLIGTLGSWFLSQKLGRFSIFFGGLVCQCIVLIVTGAIGFSNSEGASWAAGSLLLVFTFIYDLTVGPITFCAVAEMPSVRLRQKTVIIARNVYNIAGIVTQIVTPYMLNPTAWNWRAKTGLLWAAFALGAAIWTWFEFPETKGRTYAELDVLFHDGVPARKFKYTEVESFNTQEMIDKIGENGIRKIVDAAGVEKAHEVEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P291</accession>
    <name>A0A1E3P291_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b-c1 complex subunit 2, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Complex III subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Core protein II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_91130</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038722.1">
        <property type="nucleotide sequence ID" value="XM_019186278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P291"/>
    <dbReference type="STRING" id="683960.A0A1E3P291"/>
    <dbReference type="GeneID" id="30203524"/>
    <dbReference type="OrthoDB" id="6369905at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030061">
        <property type="term" value="C:mitochondrial crista"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000021">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF209">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="13">
        <location>
            <begin position="28"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ59515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="370" mass="40429" checksum="186C44B7C2FB68A1" modified="2017-01-18" version="1" fragment="single">MLSRRLARSIPSSTRGYATKVQATDFEAGKVSSLTVKVNAGSRYAPKDGLAHLLSRFNFQNTRSKSALRLTRETELLGGQFYSKVDRDAIYLTANFLKENLPYFVNALGDVLYKTSFRPHEFPEVVLPAAKYDLATANADPIFKASELLHSITYRGDLGKPLYYDGVQNVTLDDVKAYADQVYVKENIEIYGKGINPKDLDNFYKDSSLYGLPEGKSLLATSAPKTYEGVESRLRATGASVAAIAIPVKAADFATYEVLTNFLSSPLSGLSELISEVKFNKYAQSGLFTLAVKGDSSTVSSNIKQIVSSLKKSTDISVANNYTNAQIAFANDTAITPLSLDVSKVKDFKLGKFNYVAVGDVSNLPYADEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2E3</accession>
    <name>A0A1E3P2E3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_104603</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038720.1">
        <property type="nucleotide sequence ID" value="XM_019180420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2E3"/>
    <dbReference type="STRING" id="683960.A0A1E3P2E3"/>
    <dbReference type="GeneID" id="30197666"/>
    <dbReference type="OrthoDB" id="2162994at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051140">
        <property type="entry name" value="GATA_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658:SF18">
        <property type="entry name" value="PROTEIN GAT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="6">
        <location>
            <begin position="309"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="70"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="236"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="17"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="236"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="377" mass="42313" checksum="6C6C4660DBFF3EC2" modified="2017-01-18" version="1">MTDSKTTGKEIKKENLPSFTELCSSSKIQTPHQISRASDPLSSGSGTNKDAIKTLLLAFSQEIGTSIPEPNNRNYILSKSESPHETTSTRESTSNESLSNCFQPKNLALSESLIEVLHSIERSNHAHRELFPFINRIYLDLMEYVNRTKVQQEPQELFNLLFQYESLNGDPIASKISRADLNAFLQTIPIQALEDCIQNAELVKKGLCDWLQFRLNEKEKELQRLLPTPVYRHSSTPFQSTVHNSGHKPSSDYNASTTNSVSESASSSNRSSTSPERRKVDLIDEINAEKKKEKLASLGIDVEKIPTSTVVGQCCLQCDSDDTPEWRRGPYGPRSLCNACGLFYGKLVKRFGKDQAAQVMERRKSQGNGKDRRIPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2I7</accession>
    <name>A0A1E3P2I7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_30740</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038888.1">
        <property type="nucleotide sequence ID" value="XM_019181755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2I7"/>
    <dbReference type="STRING" id="683960.A0A1E3P2I7"/>
    <dbReference type="GeneID" id="30199001"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006873">
        <property type="term" value="P:intracellular monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF137">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE HRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="42"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="448" mass="50644" checksum="B76D6A8D87AC7F63" modified="2017-01-18" version="1">MKKHSSPPSKSSINIPSSSTISLSKTSTDPLAQDGILVQKYGKLGKILGSGAGGSVRLLTRPSDGLTFAVKEFRPRKSGEQLKDYAKKCTAEFCIGSTLHHPNIIKTLDIAHENGHYFEVMEYCPIDFFAVVMSGKMTRGEINCCLRQITEGVKYLHEMGLAHRDLKLDNCVVTEQGILKLIDFGSAVVFRYPFEENIVKAHGVVGSDPYLAPEVLTKREYDPQPVDIWSIAIIYCCMTLKRFPWKAPKLSDPSYKLYCMPDDNPHDYELSAKQHKELLAKKREEKLRQINAESSSNNGVEKITEVLNETTIAHDKHDEKTNDTEKSIHSTIPVSHDEKDLIHTQNDKKPIPPGEQKPHHRTIHGPYRLMRLLPHASRPIISKMLTVDPNFRATLDDVFNDEWFSSITPCTMNSKGETIHASGHHHTFVKEEEAHLQDYKDQKKPVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2Q7</accession>
    <name>A0A1E3P2Q7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Mannan endo-1,6-alpha-mannosidase</fullName>
            <ecNumber evidence="4 12">3.2.1.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_32429</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for normal synthesis of the cell wall.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>Random hydrolysis of (1-&gt;6)-alpha-D-mannosidic linkages in unbranched (1-&gt;6)-mannans.</text>
            <dbReference type="EC" id="3.2.1.101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyl hydrolase 76 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.101" evidence="4 12"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038681.1">
        <property type="nucleotide sequence ID" value="XM_019181959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2Q7"/>
    <dbReference type="STRING" id="683960.A0A1E3P2Q7"/>
    <dbReference type="GeneID" id="30199205"/>
    <dbReference type="OrthoDB" id="4187847at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008496">
        <property type="term" value="F:mannan endo-1,6-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007117">
        <property type="term" value="P:budding cell bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000006">
        <property type="entry name" value="Mannan endo-1,6-alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005198">
        <property type="entry name" value="Glyco_hydro_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014480">
        <property type="entry name" value="Mannan-1_6-alpha_mannosidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145:SF21">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DFG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03663">
        <property type="entry name" value="Glyco_hydro_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016302">
        <property type="entry name" value="Man_a_manosd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 12 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133622" description="Mannan endo-1,6-alpha-mannosidase" evidence="15">
        <location>
            <begin position="20"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="429"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="390"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="391"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR016302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ59474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="451" mass="49360" checksum="6CF6DE440129AD60" modified="2017-01-18" version="1">MRISQLFTVFAVVFTGSFAIELDIKDKDSVCSAAALVADGMLNYYEGTKYGGTVGMFSQPYYWWESGEAFGGLIDYWYLCQNDTYEQLIYDALIAQKGSKNDYIPSNQSTTEGNDDQGFWGITVLEAAERNFTNPTHGTPGWIALAQAVFNTMWARWDKDYCGGGLRWQIFTWNSGYSYKNTISTACLFNIAARLGRYTGNTTYFDIAEDAYQWLVDVKYIVEGDSAQIFDGAEITDNCTDITTIEWSYNYALMLGGSAYIYNATEDSAWLQRTEQFLEGAKVFFNDSIMYERACQGAGSCNNDQRSFKAIFSRCLGQTSVLVPSTADTIDPWIQASAEAAAKSCSGGSDGMTCGLNWFLGNWDGKYGLGEQMSALEVIQNLLIHDREAPYTNQTGGTSQGDAAAGTSGNGQSANLNSDDLTITGKDRAGSGIITAVILAVLVGGSVWMVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2U6</accession>
    <name>A0A1E3P2U6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AMP-activated protein kinase glycogen-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_91302</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRP1/MDG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038787.1">
        <property type="nucleotide sequence ID" value="XM_019186283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2U6"/>
    <dbReference type="STRING" id="683960.A0A1E3P2U6"/>
    <dbReference type="GeneID" id="30203529"/>
    <dbReference type="OrthoDB" id="3981019at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050827">
        <property type="entry name" value="CRP1_MDG1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF81">
        <property type="entry name" value="CRUCIFORM DNA-RECOGNIZING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="4">
        <location>
            <begin position="14"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="108"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="154"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ59580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="229" mass="25264" checksum="BC1D14CE0F853B07" modified="2017-01-18" version="1">MPGYTIINFPAESSNQDVQIAGNFTNWQPQVMKYNDSVSRFEYKVNDLVDGSTTGKYNFKFIINGEWRVDKEYPSEFDPSGNENNVILYDYTDDKSASDEYISKLKTEVTQDNEEGPKTPDPTPYNLSTTTTNVQTNSTSDATTATAIATGILPVTEPPTESKTLPAHDKESTVASKAVPKTEPSNGSSQFIPLTETSTEQAPKNESLSIPEPEPHLNLNLELTKNPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2Y0</accession>
    <name>A0A1E3P2Y0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">rRNA adenine N(6)-methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_31387</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>adenosine(1779)/adenosine(1780) in 18S rRNA + 4 S-adenosyl-L-methionine = N(6)-dimethyladenosine(1779)/N(6)-dimethyladenosine(1780) in 18S rRNA + 4 S-adenosyl-L-homocysteine + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42780"/>
            <dbReference type="Rhea" id="RHEA-COMP:10234"/>
            <dbReference type="Rhea" id="RHEA-COMP:10236"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74493"/>
            <dbReference type="EC" id="2.1.1.183"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8 9 10">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="10"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038972.1">
        <property type="nucleotide sequence ID" value="XM_019181829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2Y0"/>
    <dbReference type="STRING" id="683960.A0A1E3P2Y0"/>
    <dbReference type="GeneID" id="30199075"/>
    <dbReference type="OrthoDB" id="74991at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052909">
        <property type="term" value="F:18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.480:FF:000002">
        <property type="entry name" value="rRNA adenine N(6)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000007">
        <property type="entry name" value="rRNA adenine N(6)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF7">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 10" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 9 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 9 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA adenine methylase transferase N-terminal" evidence="11">
        <location>
            <begin position="44"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ59765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="323" mass="36388" checksum="B033BA6F93C7C343" modified="2017-01-18" version="1">MGKVVNKKKYSSASDNQVSAEKHMNSVFKFNTDLGQHILKNPLIAQGIVDKANIKPSDIVLEVGPGTGNLTVRILEKARKVVAVEMDPRMGAELTKRVHGTPVEKKLEIMLGDFMKTELPYFDICISNTPYQISSPLVFKLINQPRPPRVSILMFQREFALRLLARPGDSLYCRLSANVQMWANVTHIMKVGKNNFRPPPQVESSVVRIEIKNPRPAVDYNEWDGLLRIVFVRKNRTIAAGFKSSTVLEILEKNYKTYLATMDGDAMMDVDSSKKGPMIDVVKQKIEQVLTDTGLGEKRAGKCDQNDFLRLLYAFHQVGIHFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2Y1</accession>
    <name>A0A1E3P2Y1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_29749</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome b560 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038910.1">
        <property type="nucleotide sequence ID" value="XM_019181642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2Y1"/>
    <dbReference type="STRING" id="683960.A0A1E3P2Y1"/>
    <dbReference type="GeneID" id="30198888"/>
    <dbReference type="OrthoDB" id="588261at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03499">
        <property type="entry name" value="SQR_TypeC_SdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1300.10:FF:000008">
        <property type="entry name" value="Succinate dehydrogenase cytochrome b560 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018495">
        <property type="entry name" value="Succ_DH_cyt_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014314">
        <property type="entry name" value="Succ_DH_cytb556"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02970">
        <property type="entry name" value="succ_dehyd_cytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10978">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE CYTOCHROME B560 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10978:SF5">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE CYTOCHROME B560 SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01001">
        <property type="entry name" value="SDH_CYT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0874">Quinone</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ59703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="171" mass="18766" checksum="FC14E2AF5B3CDC11" modified="2017-01-18" version="1">MLSTRLGLGLIRPSSRSLISKSTITRSLQTITTTHEREQEILVAQRKNRPSSPHLTIYQPQLTWYLSSLHRVSGVLLAGAFYGVTVTYALGSILGLGIDSTVIADSFHSLSSSTQYALKGIAAFPFFFHFGNGIRHLIWDAGKELTLKGVYRTGYAVLGFSALAGAFYTFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2Y7</accession>
    <name>A0A1E3P2Y7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">General negative regulator of transcription subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_84865</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038459.1">
        <property type="nucleotide sequence ID" value="XM_019185797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2Y7"/>
    <dbReference type="STRING" id="683960.A0A1E3P2Y7"/>
    <dbReference type="GeneID" id="30203043"/>
    <dbReference type="OrthoDB" id="293823at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012270">
        <property type="entry name" value="CCR4-NOT_su3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007207">
        <property type="entry name" value="Not_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04065">
        <property type="entry name" value="Not3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005290">
        <property type="entry name" value="NOT_su_3_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-Not complex component Not N-terminal" evidence="12">
        <location>
            <begin position="3"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="249"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="40"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="117"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="249"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="278"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="291"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="385"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="412"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="438"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="573" mass="63169" checksum="57625F61BCF6DAE0" modified="2017-01-18" version="1">MAHRKLQQEIDRVFKKINEGLEIFDTYHERHENSTNPSQRDKLESDLKREIKKLQKLREQIKVWQTQNEVKDKDKLLEYRRLVETAMEKYKVVEKGSKVKAYSNMSLKQGADLDPEEQEKLDTINFLQESIDDIERQYQVVEVEIEKLSGKKSKKNSSANESKKEELKELQTRYRWHQQQLELALRLLENDELQVADILEIKEDLIYFLQSNRDPNFIDNEYIYESLDLESNELLSNEVVSSFNGLSVQEEKVEEKKEKSISPKPAKSVTANTPEKVSPAPSSAPALSGLPPAPPASAPAQGPPGINPIRRSETPKKKFVSLSTNSNTSTPTSLSSTLKPAAPPSQPPSAMKWSILASGGFLNNNGNGNGDSEAVPSSPAPPSTAPAQITTATSSNATEVPITPSKSIPPATTTTTTTTTTSTTAGVPSSIGLERPATSVSPSEGVSNSLNGNVSIGDTARSSSSNLPISPVNQVEYQQDVNLLKNPPGINAMIASSISSRTSSNNGEFPKSTANVRDLLRLPRDYMVSQVEPSQLTTGEYQRVAQLYEDARHKLSMTNNFDAVLTLHQLNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3I4</accession>
    <name>A0A1E3P3I4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ESCRT-II complex subunit VPS25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_31311</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS25 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039182.1">
        <property type="nucleotide sequence ID" value="XM_019181818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3I4"/>
    <dbReference type="STRING" id="683960.A0A1E3P3I4"/>
    <dbReference type="GeneID" id="30199064"/>
    <dbReference type="OrthoDB" id="245150at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904669">
        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000141">
        <property type="entry name" value="vacuolar protein-sorting-associated protein 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.570">
        <property type="entry name" value="Winged helix' DNA-binding domain. Chain C. Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008570">
        <property type="entry name" value="ESCRT-II_cplx_Vps25-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014041">
        <property type="entry name" value="ESCRT-II_cplx_Vps25-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13149">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13149:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN-SORTING-ASSOCIATED PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05871">
        <property type="entry name" value="ESCRT-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ59975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="189" mass="21643" checksum="900F90E2D4623C21" modified="2017-01-18" version="1">MPNYTFPQIYNFPPFFTRQPNEQTWHAQRSSWIQLILGYSRANRVWILNNKGQVLSQGNVDEGATITKESLFANDRIQRSLSIETVDEIFSEMVKSEDAFYNDNHSSVFVNFYRPEDWAAMVLEWVESTGQSGGVLTMYEIANGELSASQEFHGIHPVILEKALNVLVKRSRAQLLKDQDNRIAGVKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3I6</accession>
    <name>A0A1E3P3I6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_62607</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3I6"/>
    <dbReference type="STRING" id="683960.A0A1E3P3I6"/>
    <dbReference type="GeneID" id="30200649"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133661" description="Hyphally-regulated cell wall protein N-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="249"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="34492" checksum="03E0C663F4E27515" modified="2017-01-18" version="1">MKFSTRFFISLSSLANIIIAASEEFTLKLQADGTALAGDNIHISSDNEFFISDTDQTATGQIEDDGTLKINGYTVGVGKNYLSLTADSSSFMTAEPWSIVGGQLKLYGSNFHAVPSGQNGIYVLGTINAASGRSDVINVAIEAQGSNGKSVADYDASVKSSSVQSSSSSSTEASITPVSSAEQTTTSTSSSIVESTNKKETSSTVASSVKTSVFSSEPEPSSEGTTSAVPESSSQLPASSSTQEEVPKSSTAAAPSSTDMSSSTIIDSISSAIESTSSAVGSTGSPAITSSSASVARSATFSTVSNSNLSSSAVSIQTFDNAGMKKGASVFAAVVAGIALF</sequence>
</entry>
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            <fullName evidence="6">B30.2/SPRY domain-containing protein</fullName>
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    </protein>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
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            <taxon>Saccharomycetes</taxon>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ60059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3P3K6"/>
    <dbReference type="GeneID" id="30202017"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="B302_SPRY"/>
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    </dbReference>
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        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="2">
        <location>
            <begin position="173"/>
            <end position="376"/>
        </location>
    </feature>
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        <location>
            <begin position="472"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="632" mass="71189" checksum="1D613D5B43C319DE" modified="2017-01-18" version="1">MESSTSNAAVAAAAAAAELNQQQPQQEFHLPSYLLKTAYGEELQKKLATPSTLQNDRSITTKLSALGIMGNMPSRYLNADYDSELASIFSATTNSQSTNPSRDRNTTFDSYEDDDVIHDDEDEEDDDDDEEEEEEEVRNIHYADEMDIDYEDAGIRRSISSRSISRRNSRKDSRSSRLQSISSTKNPFTNFKYSLPTKWEPSSNKSLVVANNGLTLKIDNNSNNSATASISKNYDYLVTKTDNSIPVNVGIFYFETKILTVTNNNLKNNSDVTIGFMHSSLGNVSKMPGLENGSYGFNGSDGSIIYSQSTSKKYNCKFGQGDIIGTGINFATKSIFYTKNGVYLGVAFTDINRALTPIIGLKNGNSVSSNFGQNEFIFDIDGYIDDEKLKIYKKVFSFTKDNKCDAVNRINLNTDKEIPNVLQKLVSSYFNHLGYIDISKTFLQEIKNEQVDKTLIKNFNKIETISTIDENNLKIRQQIRKYLISGDIDSSIKLTNLNFPKVFSKNEEILFKLNCNEFIQLIKKAKIDEAIEFGQSLRSKYSENSKFQEFLNDIFSLLAFENPKESEFGYLLNDDEILKISDELNSEILKSLGKSSISNLEKLIKHNKSMLHILNDNNQLDSLLITSDDFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3M7</accession>
    <name>A0A1E3P3M7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2-(3-amino-3-carboxypropyl)histidine synthase subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_30883</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase. Facilitates the reduction of the catalytic iron-sulfur cluster found in the DPH1 subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the DPH1/DPH2 family. DPH2 subfamily.</text>
    </comment>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11860:FF:000001">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010014">
        <property type="entry name" value="DHP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00272">
        <property type="entry name" value="DPH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF2">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01121">
        <property type="entry name" value="Diphthamide_biosynthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00408">
        <property type="entry name" value="Diphthamide_biosynthesis_famil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="418"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="427"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="547" mass="61192" checksum="87FCB7DAA5CF75C5" modified="2017-01-18" version="1">MTEIVAPALSTHQDDNTFTYDKYQDKQKDRSYLGPNWNDEQSLREKLIEFYSIDELVVHLGQRASDGSFKFKKITLQFPDSLVADAAIVVQLLQEKLGNASKAQDNTQQVWILADTAYSPCCIDEVAAEHVLSDIVVHFGDACMNAIQKLPAVYVFGKPYLDIDTIVGKFKATYPNESSKVVLMADTSYSRHLPELFNILKETYKDVAYADISPERASNAKIIGFKSTNQEGSIRFSNRKLITSSDEALEDENLQDYALFHISTPEPPHLLYLSTKFGSVTLFDTINNSINQGPFPSLMKRYRYMHIARTAGTIGILVNTLSLRNTTEVINKVSRKIKEADKKYYMFVVGKPNVAKLANFESIDVWCILGCGQGGIIVNNNNDFFKPIITPYELEMALSPEVQWTGKWVTEFENVINDEESNEDSEASTSLDDTNSPTLEGEDEAPEFNAVTGKYVSNSRPMRQIQHLEIDAVDSTVEQENQLVKKFSQAVAIKGTVSTSAIHLQNREWTGLGSDFVDNDVDDEGALVEDGRGGIARGYDFDVKGSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3N0</accession>
    <name>A0A1E3P3N0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_56243</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the THADA family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038694.1">
        <property type="nucleotide sequence ID" value="XM_019182777.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P3N0"/>
    <dbReference type="GeneID" id="30200023"/>
    <dbReference type="OrthoDB" id="73997at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002130">
        <property type="term" value="P:wobble position ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056843">
        <property type="entry name" value="THADA-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056842">
        <property type="entry name" value="THADA-like_TPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019442">
        <property type="entry name" value="THADA/TRM732_DUF2428"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051954">
        <property type="entry name" value="tRNA_methyltransferase_THADA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387:SF0">
        <property type="entry name" value="DUF2428 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387">
        <property type="entry name" value="THADA/DEATH RECEPTOR INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10350">
        <property type="entry name" value="DUF2428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25150">
        <property type="entry name" value="TPR_Trm732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25151">
        <property type="entry name" value="TPR_Trm732_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like TPR repeats region" evidence="4">
        <location>
            <begin position="226"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2428" evidence="3">
        <location>
            <begin position="576"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like C-terminal TPR repeats region" evidence="5">
        <location>
            <begin position="794"/>
            <end position="927"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1327" mass="152647" checksum="1BD066B1626C6117" modified="2017-01-18" version="1">MAGDMISLENPPCEGVQELTIDELTSLRRFFIAQKSVQVDHYDDFLIKTFPLLIRSLKSATGVGNYRITCTDTIAIWLLRASQLCSKCPKFEKTIGDMIFANDDSTFLFRYVVDYCNETGAPLSNSLRDVFTKLLTLLKRYNPQELFRSWADETLGISHGRRVQYYLVELLCKEIEQNDYFIIKDPSFVSDTIQLMSSNALANPIGKAVSAIYKKLYTDEDHAEEWVSLYWSPIKQGIKNPALRRGIEQYILPNLFKFSKTAFKVFVNDLQDDIDLFITCLNIGQTLAIEEDPFPNLVSLDVVNKLLVNDAYKIKIFQMLTYSPKGSRPISSEVFDIMRNNLDVYFTDCEVETRNEFHSLFKQFIFRIKDSAYALHRDATKLEKKSMLKEAQLKFEQVNIIKSFLKWLLSFIKEQIKPGAQYQRVSTGFKILQFLADTGLDSKISVKLDIYYPFNIEMFDESFIRLFFDNILSTYEDTRMNAVSLLVLSKITLDEKHKCILIEKGIHMLGDYTKSEAGAMILECLYLLQNDDLILKNLISNIPLNTDIKQAVYSPVHGYFEAMSLILKRMNKFSDCEQVIQIVEKNWETVREILSHDSPEGCDQYGVGSAQLVLSYAWRATKESTILLQRLLDFELTKSQLLRVGELALDQLSTVRHRGAFSAVYPTFIKVASICQKMVTDQSKKWLDFNISLIQTKTQLITRRSGGLPFLITAIVTVERDLLRYTFDRLLEIAREPVDEKAESEKMDIAQVHAFNCIKTLFIESQLATSVAPYIYEGLELALSTFSSKIWSVRNCSIMLFTALQNRLFGRKKMSARVFFSRFKGIREILIKILKSSIAEGNLETLFPVLTILSKLEANPGYDGLDEFKPLLRVCFATKYWKIREAAARAMPALITDPNQEAVSLLETCSIKNKNKMHGHLLAIKSLNVYSEELVKQLYLRFDEFFIHNKCYSTSMIYVQLLSAACKEFPNEEITERTRIWFSENNSQFEIDGSRHLLMKEVFKFISSNDVDLLNQGLRSPFFEVQLEAIDFCAKSKAQLPALSEIANDDDCWTYVRSKAIPLVEDYSTNKIFEFVEKQDVYGEDIKRSSLELLGSIAAKSHNCQIYESWYKLAVKNADDNEPYQIRLSALKSVLRFLNERKTSDVLWLLYNFLSDDDDEIRELASTHFGGNSVSWYDGDQFSQNFGKDELSAHVVLHQALKFNPSFSPTSGNDKVLFNVEKANFYRNKSELHTQLARMVQNSIEYLADHDTKLLKSHFSEMSDKLVKFMKEKGYDGILGWSTEEEVFEDIYGVISYLKALNMDTSNILLTAKQVQLHDYLIKLLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3W6</accession>
    <name>A0A1E3P3W6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_18875</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN12 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038799.1">
        <property type="nucleotide sequence ID" value="XM_019180809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3W6"/>
    <dbReference type="STRING" id="683960.A0A1E3P3W6"/>
    <dbReference type="GeneID" id="30198055"/>
    <dbReference type="OrthoDB" id="10252687at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045114">
        <property type="entry name" value="Csn12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732:SF0">
        <property type="entry name" value="PCI DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732">
        <property type="entry name" value="UNCHARACTERIZED PROTEASOME COMPONENT REGION PCI-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="2">
        <location>
            <begin position="155"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="345" mass="40290" checksum="03AE9456D30795C9" modified="2017-01-18" version="1" fragment="single">SFNTGNELLKNLNRFAEQKTNWIVKSLIVITKNLITYAIKADEYIEKNPTVYLSRKNLGDFEHESCLIKAARTIHNSFKLCLNDRNEEIYQSRRNSVYFFVGQELKIYFNLQNRDLAKNMEKVLISKQQDLPDLSSIDKSQAITYLYYSGAIACGEGDFKTAYLKFKYAFQLCSKNDLKHKESILIYLIPLKFLITKKYPNLIKLRLFRKNYEIYQPILSSLLIGDLKNFDKNFNQFEKFFLKKNLYLVLEKLRTFVVLKLIKKIYRLEGSSSHLKIPIIARGLEFSINHINNENLTRKKNAISIFSTDEAECILANLIYSGYMKGYLSHTNGILVLSKKDPFPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P411</accession>
    <name>A0A1E3P411_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ER lumen protein-retaining receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_92071</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ERD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039187.1">
        <property type="nucleotide sequence ID" value="XM_019186337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P411"/>
    <dbReference type="STRING" id="683960.A0A1E3P411"/>
    <dbReference type="GeneID" id="30203583"/>
    <dbReference type="OrthoDB" id="7694678at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045015">
        <property type="term" value="F:HDEL sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000133">
        <property type="entry name" value="ER_ret_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10585">
        <property type="entry name" value="ER LUMEN PROTEIN RETAINING RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00810">
        <property type="entry name" value="ER_lumen_recept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00660">
        <property type="entry name" value="ERLUMENR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00952">
        <property type="entry name" value="ER_LUMEN_RECEPTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ59980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="148" mass="17680" checksum="C362D86E399D9291" modified="2017-01-18" version="1">MFFIGSSIYIVYLMLFKFKNSETQKIDNFKIEYLIGGSFLLSLIFNYGFQFTEILWSFSLWLESVAILPQLFMLQRTGEAQTLTTHYIFALGIYRALYIPNWFYRWFAEGRFDALAVFTGIIQTLIYSDFFYIYYNRVLKGLKFELPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P418</accession>
    <name>A0A1E3P418_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_78265</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038834.1">
        <property type="nucleotide sequence ID" value="XM_019184692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P418"/>
    <dbReference type="STRING" id="683960.A0A1E3P418"/>
    <dbReference type="GeneID" id="30201938"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45776">
        <property type="entry name" value="MIP04163P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45776:SF2">
        <property type="entry name" value="MIP04163P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="213"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="38"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="120"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="295"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="54"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="120"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="205"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="352" mass="39536" checksum="926E3BDF1863B591" modified="2017-01-18" version="1">MTDEYLNFNSGKINLDDFSFNINNDASSTIPENELLDFTTGNPNNNNINNSHNLHQGSHGTTHLNPTQLHSGSLTHNDPSNVQNSFHNNSYLSPVNSPYTNPNHLSTSFVSSRKNSVYDTLESVASPQQHQSTLNSQYFSPSQKPMSFSNNTASRQIPDISSINSPGSINDQLSPYPSSFDTFKSPSVRSQGLSPATSSGPKQQLTKEDKLRRRREFHNQVERRRRDLIKERIKELGVIVPPSLLLVDSDGKEIKPSKSVIINKTVEYLIHLHKVMKSQDERLADLVAKIEELEKLPDQNPVPQGNNESSYQNSPANIQTFGSDNQQHELDFDVNEFLKDHKTDANWDGLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P444</accession>
    <name>A0A1E3P444_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Centromere protein H C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_62551</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the CENP-H/MCM16 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039181.1">
        <property type="nucleotide sequence ID" value="XM_019183394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P444"/>
    <dbReference type="GeneID" id="30200640"/>
    <dbReference type="OrthoDB" id="2274804at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008426">
        <property type="entry name" value="CENP-H_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05837">
        <property type="entry name" value="CENP-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centromere protein H C-terminal" evidence="8">
        <location>
            <begin position="100"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="252" mass="29097" checksum="FCD566D9CBB2A820" modified="2017-01-18" version="1">MSKELKNSVLKLLKESGVLEITDDPAIKEIQTINNDDISTEIKHTQVYRDLVDNLDRLMVIEQSQKGMYDSVKDELMKSGRLAHLIQDGELKDEELLKYLKQIQQIRRLVIESSTMTLPVLRSVHQVEGLTTKEAGIVSKIEKRDELTDKIIKLQFKINKNTEKLIKLTNENLSKFETSKQLTSLLIETKRNDNTPQDPKQKAILEKNLAEAKEKSYNLAEQIQAFIVENGLNWADDEQLREIVLYCGEIYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4Z3</accession>
    <name>A0A1E3P4Z3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_83970</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039179.1">
        <property type="nucleotide sequence ID" value="XM_019185631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4Z3"/>
    <dbReference type="STRING" id="683960.A0A1E3P4Z3"/>
    <dbReference type="GeneID" id="30202877"/>
    <dbReference type="OrthoDB" id="191150at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903147">
        <property type="term" value="P:negative regulation of autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF9">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="23"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="309" mass="35201" checksum="70A1E15CB17F623F" modified="2017-01-18" version="1">MNILRQSIRRPFPRPSLTNLIKPRFYSPLPRSSRSSGYRGGRFPEQQTVEDIGTEPDVNRYSFDSYSGTVPNPGGLLRETDGISKILTEPTIVIERFEQANQYVIMDALGNKIGYMSERDFGIGKMIMRQITRLHRPFIVDVFDNFGNPVLTIKRPFSFINSHIKAILPGFEIEDDGIIGESVQSWHLWRRRYNLFKAHSNNEYDQFGAIDSGFLAFDFPVRDENGRVIGAVDRNWVGLGRELFTDTGVYIVRMDPQSFAGMGELYPEVSGPLTLDQRAILLANAVSVDFDYFSRHSNRGGLFSFGDYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P537</accession>
    <name>A0A1E3P537_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">MATE efflux family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_84265</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039597.1">
        <property type="nucleotide sequence ID" value="XM_019185681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P537"/>
    <dbReference type="STRING" id="683960.A0A1E3P537"/>
    <dbReference type="GeneID" id="30202927"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048279">
        <property type="entry name" value="MdtK-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="453" mass="50021" checksum="C9CAC983CE50A738" modified="2017-01-18" version="1">MIIGKSAIPLMFTMFLQYSINAVSILSVGHIGENELAGVSLANVTFVVTSSIFIGMATCLDTLCPQAYGAKKYHLVGTYLQRCVTLCFIISIPVIIVFYFSGSILKHVVPNEELAYLAQSYLRIVSIGLPGYILFECGKRFLQAQGNFFGGQYTLFFCAPLNAIMNYLFVFKLGMGYCGAPIAVALNFWIMAILLFSYCVLIDGNQCWYGFQWKQCFKGWKAMIDLALPGILMIEAEFLAFQIITLSCSRFGTTALAAQSVVSSMASLSFQIPFSISIAASTRIGSLIGSNSKEASKISVNVTLFYSLFSGLWNFSILLVFRSQIAGIFTSDPIVISKAVYVFPIVALNQFYDCFNVLAAGCLRAQGRQKIGGYLNLAAYYLIGLPLAMLLGFKYGYEIRGLWGGLGVGIFVLALLEIFYIYTSDWDRIIETSIQRHKQAESDKIIDENTTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P545</accession>
    <name>A0A1E3P545_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 18">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="6 18">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">WICANDRAFT_68891</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines. Negatively regulates the biosynthesis of acetyl-CoA from pyruvate in the mitochondrion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 19">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="6 18"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039607.1">
        <property type="nucleotide sequence ID" value="XM_019184143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P545"/>
    <dbReference type="STRING" id="683960.A0A1E3P545"/>
    <dbReference type="GeneID" id="30201389"/>
    <dbReference type="OrthoDB" id="275353at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00318">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000003">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000019">
        <property type="entry name" value="Phosphoglycerate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="entry name" value="Phosphoglycerate kinase, N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406:SF0">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="11 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="24"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="63"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="371"/>
            <end position="374"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00016471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ60400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="415" mass="44618" checksum="94B7A61ABD13B464" modified="2017-01-18" version="1">MSLSSKLSVTDLNVENKRVFIRVDFNVPLDGTKITNNQRIVAALPTIQYVAEHKPKAIILASHLGRPNGERNEKYSLKPVVAELSSLLGKQVTFLNDCVGDEVEKAVNGATDGQIFLLENLRFHIEEEGSKKTSEGKVKASKEDVEKFRKQLTSLADVYVNDAFGTAHRAHSSMVGFDLPQRAAGFLMAKELTYFAKALENPVRPFLAILGGAKVSDKIQLIDNLLDKVNILIVGGGMAFTFKKVLDNTKIGKSLFDAEGAKIVPQLIEKAKKNNVEIVLPVDFITADDFNKDANTGYATAEEGIPDEWQGLDGGPKTRELFAAAVAKAKTIVWNGPPGVFEFEKFAEGTKSLLDAAVKSAANGNTVIVGGGDTATVAKKFGAADKLSHVSTGGGASLELLEGKELPGVTYLSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P567</accession>
    <name>A0A1E3P567_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="14">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_29087</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="14">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="14"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039816.1">
        <property type="nucleotide sequence ID" value="XM_019181575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P567"/>
    <dbReference type="STRING" id="683960.A0A1E3P567"/>
    <dbReference type="GeneID" id="30198821"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001100">
        <property type="term" value="P:negative regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007231">
        <property type="term" value="P:osmosensory signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016241">
        <property type="term" value="P:regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033262">
        <property type="term" value="P:regulation of nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051403">
        <property type="term" value="P:stress-activated MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07856">
        <property type="entry name" value="STKc_Sty1_Hog1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000049">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000050">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008352">
        <property type="entry name" value="MAPK_HOG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038783">
        <property type="entry name" value="MAPK_Sty1/HOG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01773">
        <property type="entry name" value="P38MAPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="22"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ60609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="352" mass="40652" checksum="4AC387EA62EE36AA" modified="2017-01-18" version="1">MSQEEFIRTQIFGTVFEITNRYTDLNPVGMGAFGLVCSAKDKLTNQNVAIKKIMKPFSTPVLAKRTYRELKLLKHLRHENLITLEDIFLSPLEDIYFVNELQGTDLHRLLTSRPLEKQFIQYFLYQIIRGLKYVHSAGVIHRDLKPSNILINENCDLKICDFGLARIQDPQMTGYVSTRYYRAPEIMLTWQKYDTEVDIWSAGCIFAEMIEGKPLFPGKDHVHQFSIITELLGTPPDDVIDTIASENTLRFVKSLPKRDPIPLTERFKNVEPDAVDLLEKMLVFDPKKRITAADVLGHPYLAPYHDPTDEPVAEEKFDWSFNDADLPIDTWRVMMYSEILDFHQVDNNGGVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P594</accession>
    <name>A0A1E3P594_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRELI/MSF1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_40009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039846.1">
        <property type="nucleotide sequence ID" value="XM_019182549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P594"/>
    <dbReference type="STRING" id="683960.A0A1E3P594"/>
    <dbReference type="GeneID" id="30199795"/>
    <dbReference type="OrthoDB" id="407630at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037365">
        <property type="entry name" value="Slowmo/Ups"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11158">
        <property type="entry name" value="MSF1/PX19 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="232" mass="27267" checksum="7B8157F378F928EA" modified="2017-01-18" version="1">MKIFSNSCEFYYPWEQVTAANWRKYPNEMSTHVVAVDVLRRELDPTCKILTTERLITCKQSIPSWLLCMVGGKEVSYVREVSTVDMNTKTLTLRSINLTLNNLLKVYETVTYKPDQTDPFKTCFNQEAQITAYATFQRICNKIEDWSVERFGENAKKGKIGFDSVLKIFNEQWERRDELVDEISDKVVKTYDDLKIEIVKETDKILNEAEHVKKSILIEYYDIIKKAFKRDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5A8</accession>
    <name>A0A1E3P5A8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Chromatin assembly factor 1 subunit A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_61226</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039866.1">
        <property type="nucleotide sequence ID" value="XM_019183133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5A8"/>
    <dbReference type="STRING" id="683960.A0A1E3P5A8"/>
    <dbReference type="GeneID" id="30200379"/>
    <dbReference type="OrthoDB" id="79480at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044389">
        <property type="term" value="F:ubiquitin-like protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048800">
        <property type="entry name" value="Cac1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021644">
        <property type="entry name" value="CAF-1_p150_acidic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022043">
        <property type="entry name" value="CAF1A_DD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050899">
        <property type="entry name" value="DDRGK_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48176">
        <property type="entry name" value="DDRGK DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48176:SF1">
        <property type="entry name" value="DDRGK DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21796">
        <property type="entry name" value="Cac1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11600">
        <property type="entry name" value="CAF1A_acidic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12253">
        <property type="entry name" value="CAF1A_dimeriz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromatin assembly factor 1 p150 subunit acidic region" evidence="2">
        <location>
            <begin position="103"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Chromatin assembly factor 1 subunit A dimerization" evidence="3">
        <location>
            <begin position="310"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Chromatin assembly factor 1 subunit Cac1-like C-terminal" evidence="4">
        <location>
            <begin position="463"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="350"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="434"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="61403" checksum="EA593A0061A1D8C8" modified="2017-01-18" version="1">MADTSQPSISQLLMQSSTERQPEDVVELLSSQPKEEVNIEPVVPDARAESPTKLPDYIQATPAITPSKVPIQIINPVTASSGDAKSPAPTTPAKTPKQKQKEEEKLQRMKQRELEKQEREKAKEEERLLKEKLRKEKEEERKRINDEKERIKKEKREKLLEEKKERDRKKEEERLKKEEEKQKKLDEKRKAEEQKKKEEEAKQKKATKLQISNFFKAKTTLKSTIAPAAVDTSDADYKIAFKNFYLRDNLSLKVSPTLCDVEDFDEQLKTSKGQGLKEWFKQRENSNVQESITAKYIFTHQLDSAPLNFKFIRFYENIKTYIGTYTKDPTTGISIDPFILTDAFIDWDEEENENEEGEGEDIDDEDDEEEEDEDMGDLSDFLDEDGEENKNKKKTLGPLIPQIHWKDITEYSIEVLKPDLITINPFEDYWKTTDTKDSGLPGDQDQSPKPKKSKSLITSRDDLEKFAKQVHDSDFTVPTMVELLKKQLPTYTKLTIENTLKNYAKKVGPKATEKKWEVDTELLKKAMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5F8</accession>
    <name>A0A1E3P5F8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_77352</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039882.1">
        <property type="nucleotide sequence ID" value="XM_019184542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5F8"/>
    <dbReference type="STRING" id="683960.A0A1E3P5F8"/>
    <dbReference type="GeneID" id="30201788"/>
    <dbReference type="OrthoDB" id="3251668at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052360">
        <property type="entry name" value="Transcr_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36206">
        <property type="entry name" value="ASPERCRYPTIN BIOSYNTHESIS CLUSTER-SPECIFIC TRANSCRIPTION REGULATOR ATNN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36206:SF12">
        <property type="entry name" value="ASPERCRYPTIN BIOSYNTHESIS CLUSTER-SPECIFIC TRANSCRIPTION REGULATOR ATNN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="449"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="290"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="326"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="383"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="290"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="331"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="351"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="405"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="533" mass="60489" checksum="9BC1D5D2C5D2A344" modified="2017-01-18" version="1">MSSTSFGTTSKKNKNKSNPAITDYDRSKLKAHREQLQKEIDRIAGLLKGKDVHNSPIDSLASIASGAPSNTPVHSPKRYSYSSSKNYITGRPLRSQVKPFQLQASLQEDESHNLQLDDNHRRSSNSKFLSINDLLSSENNFSTLHHESSPLGDKTLDFINQTSVTANNTLFHNYSGNVQVDSTKKTDVGETSNTIVPIDVLEDRKSHVAKGVDISITSSQNRSRFDPNYSSPFVSRTSMKRETFFTNNYSNVLDYEVSKNLLALSSSPMPKFETRFPDPRPIHELFESVPNMDDHLREDDERTNSVLPSDEEEEFNIKEVDIENLDDDHEDFGFKSDKIDEDLKKPDKNPVYSSSPQGPKFEVSSNIRLALRKRKPVNYCIESTEGDKLGDSPKTSRLKRRTLRSLKQTKQQVSFASSSPAKRTSSSGSNKSSCYHRRKKSFLPRSKSGCWTCRIRKKKCTEEKPSCIQCENLGLKCDGYSDEKPLFMHNAQLQRKRLDEIKDHTSKRKKIGIKKAKFTLGNDNNYDGDENYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5I4</accession>
    <name>A0A1E3P5I4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_89870</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039923.1">
        <property type="nucleotide sequence ID" value="XM_019186199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5I4"/>
    <dbReference type="STRING" id="683960.A0A1E3P5I4"/>
    <dbReference type="GeneID" id="30203445"/>
    <dbReference type="OrthoDB" id="10067394at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="22873" checksum="2832A5333F18EC21" modified="2017-01-18" version="1" fragment="single">MIPNQNLRDSQHWYFLAEKFWDLLDLESSLQAMVLLSGSLGPGGYVQQAIEGSERLLRLMNFNKVLTTLQIDTDYESYAQVATKRQMLLREDLLRTIWGSWKLNVFLRINRGFPFNQISDQLLGFEPNLQFPSSDSAYNVNLSLFDTNDNISPNSLKYRTWKDIEEELRLASTNSAVYKSFNDADLVIMAIKTVEDIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5J9</accession>
    <name>A0A1E3P5J9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_68730</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039409.1">
        <property type="nucleotide sequence ID" value="XM_019184136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5J9"/>
    <dbReference type="STRING" id="683960.A0A1E3P5J9"/>
    <dbReference type="GeneID" id="30201382"/>
    <dbReference type="OrthoDB" id="432719at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000001">
        <property type="entry name" value="ATPase copper transporting beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000005">
        <property type="entry name" value="Copper-exporting P-type ATPase A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006122">
        <property type="entry name" value="HMA_Cu_ion-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00003">
        <property type="entry name" value="copper ion binding protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46594">
        <property type="entry name" value="P-TYPE CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46594:SF4">
        <property type="entry name" value="P-TYPE CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="3">
        <location>
            <begin position="2"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="3">
        <location>
            <begin position="79"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="3">
        <location>
            <begin position="169"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="337" mass="36012" checksum="05CFCDDE03D6EE9F" modified="2017-01-18" version="1">MVNTVLTVSGMTCGACSSAVTNALTSIDGVQDASVSLITEEANVKHSSSVSATDLQTAIEDCGFDAIVISSEDETPRVFETKLSVKGMTCGACTSSITSMIEPKAGVQKFDISLVTEEAVVKHDDTIKPSQLKEWIDDAGFDAEILESREITNGTSGSLPNKPTGLEQAETTISIKGMTCGACTSSVTNVLNDIEGVLDASVSLVTEEARVKHFKTLNPSVLKDAIEDCGFDAEIVDTVTQNNQSPLIETTKFTVDSDLDLVDLEQQMSQFKGYISSSLIDEYSIAVTYDSAKTGVRFLVRDFQSIGVIAQPQNIMKIGLESKRLIKGIMTLIFASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5X9</accession>
    <name>A0A1E3P5X9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GTP:AMP phosphotransferase, mitochondrial</fullName>
            <ecNumber evidence="7">2.7.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Adenylate kinase 3</fullName>
            <shortName evidence="7">AK 3</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ADK2</name>
        <name evidence="10" type="ORF">WICANDRAFT_83158</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a ribonucleoside 5'-triphosphate + AMP = a ribonucleoside 5'-diphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:13749"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon GTP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent GTP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adenylate kinase family. AK3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.10" evidence="7"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040042.1">
        <property type="nucleotide sequence ID" value="XM_019185499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5X9"/>
    <dbReference type="STRING" id="683960.A0A1E3P5X9"/>
    <dbReference type="GeneID" id="30202745"/>
    <dbReference type="OrthoDB" id="439792at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046899">
        <property type="term" value="F:nucleoside triphosphate adenylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006172">
        <property type="term" value="P:ADP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046039">
        <property type="term" value="P:GTP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046041">
        <property type="term" value="P:ITP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000106">
        <property type="entry name" value="Adenylate kinase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03169">
        <property type="entry name" value="Adenylate_kinase_AK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007862">
        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028586">
        <property type="entry name" value="AK3/Ak4_mitochondrial"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Adenylate kinase active site lid" evidence="9">
        <location>
            <begin position="138"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMPbind" evidence="7">
        <location>
            <begin position="38"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="7">
        <location>
            <begin position="137"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="17"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="65"/>
            <end position="67"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="96"/>
            <end position="99"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="147"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ60835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="227" mass="25349" checksum="16100E8885589CCB" modified="2017-01-18" version="1">MSLPSRPLRLLLLGAPGAGKGTQTSKLLEHYTGLLPISSGDLLRREIQNKTEIGSYASGIIAKGGLLPDFVISGLIFNELKDKSLLNSKASWLLDGFPRTLPQARLLTEDLTKKDANLNLVVELKVPEKVILERIENRWVHIPSGRIYNLQYNPPKQFGIDDVTGEPLSKRPDDNAEVFQQRLNAYHETINPVKDFYKGLGILHAVEGETSDIIFPKLLDLIQKNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P673</accession>
    <name>A0A1E3P673_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CN hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_89988</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039997.1">
        <property type="nucleotide sequence ID" value="XM_019186204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P673"/>
    <dbReference type="STRING" id="683960.A0A1E3P673"/>
    <dbReference type="GeneID" id="30203450"/>
    <dbReference type="OrthoDB" id="10250282at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050152">
        <property type="term" value="F:omega-amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006528">
        <property type="term" value="P:asparagine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07572">
        <property type="entry name" value="nit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045254">
        <property type="entry name" value="Nit1/2_C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001110">
        <property type="entry name" value="UPF0012_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088">
        <property type="entry name" value="NITRILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088:SF30">
        <property type="entry name" value="OMEGA-AMIDASE NIT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01227">
        <property type="entry name" value="UPF0012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="2">
        <location>
            <begin position="11"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="293" mass="32424" checksum="3F438311AC7D49A2" modified="2017-01-18" version="1">MTTTTPLTKKLHLALLQFAVSSDKLANFHKVESLVSKAVKAHPNLDLIVLPECFNSPYSVKLFKKYGEDIPNGETTKFLSSLANKYQINVIGGSYPEAHHNQVFNTSTVFNKSGDLIAQHRKAHLFNIDIPNKITFQESRVLNAGDKHTQFALPGFGSLGLGICYDLRFPELAMMAARRGAFAMVYPGAFNTITGPLHWEALARARAIDNQLYVVLCSPARDLSAKYHAYGHSLVVDPMGKVIVEAGEGEETIYCELEPELIAETRRSIPVNEQRRFDIYKDVGDGGEIGDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6X7</accession>
    <name>A0A1E3P6X7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone acetyltransferase type B catalytic subunit</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_61616</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040257.1">
        <property type="nucleotide sequence ID" value="XM_019183214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6X7"/>
    <dbReference type="STRING" id="683960.A0A1E3P6X7"/>
    <dbReference type="GeneID" id="30200460"/>
    <dbReference type="OrthoDB" id="10253098at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.360.10">
        <property type="entry name" value="Histone acetyl transferase 1 (HAT1), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019467">
        <property type="entry name" value="Hat1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037113">
        <property type="entry name" value="Hat1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017380">
        <property type="entry name" value="Hist_AcTrfase_B-typ_cat-su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013523">
        <property type="entry name" value="Hist_AcTrfase_HAT1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12046">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE TYPE B CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21184">
        <property type="entry name" value="HAT1_C_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10394">
        <property type="entry name" value="Hat1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histone acetyl transferase HAT1 N-terminal" evidence="9">
        <location>
            <begin position="8"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ61050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="389" mass="45636" checksum="2BE768B6B15F6937" modified="2017-01-18" version="1">MSYNPEVWTISSNEALKVSIADDQGYVSFPPLFTYPIFGDSEQIFGYKDLKIDLVFDSITFLPFLKVSYKEKLNDEVDDITAKLLEFLPKSTIVDDEVKWVDAFKKEQEELIDYNKEFELINTYNVKNEEFSIFKTSFQTSEGEKTKAVDLMKRLQIFVLLFIEAGSYIDTSDELWDLFILVSNKTKKILGFSTAYSYFKYNGAIEFDSNPNVKKRNKISQFVVLPPYQKTQHGSSLYSSIISYWNGNELVEEITVEDPNEKFDDLRYRNDFKRLFEEGFINQLPKNLKTIDNKWFITNANKFKIELNQFKKLVEIGYLYTENIKLARLLIKKRLYEKNRDGLVELELAIRNDKLQTAYESIKQEYDRLIDSIRVRAVNDIGSSKKQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6Y6</accession>
    <name>A0A1E3P6Y6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Lysophospholipid acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_28897</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040393.1">
        <property type="nucleotide sequence ID" value="XM_019181548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6Y6"/>
    <dbReference type="STRING" id="683960.A0A1E3P6Y6"/>
    <dbReference type="GeneID" id="30198794"/>
    <dbReference type="OrthoDB" id="286734at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044233">
        <property type="term" value="C:mitochondria-associated endoplasmic reticulum membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071618">
        <property type="term" value="F:lysophosphatidylethanolamine acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030258">
        <property type="term" value="P:lipid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036151">
        <property type="term" value="P:phosphatidylcholine acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090640">
        <property type="term" value="P:phosphatidylcholine biosynthesis from sn-glycero-3-phosphocholine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF4">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="417"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="453"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="516"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="581" mass="67712" checksum="1F4345A3719308B9" modified="2017-01-18" version="1">MLNPISSQIDLWAEAIGLDEATMKLAICLFGSFPLNAILKRLPDNNIRLKNIYIISISGFYLFGIMSLFSGFRTLFISTMFTYLCTKYLKSDFMPFINFAFVMGHLAMNHFYLQFYNEYDSTKIDITGAQMVLCMKLTGFAWNVQDGRKPESELSTFQKNKAIKSHPPLLNFLSFSFFYPSLLTGPSFDYIDYESWLNSKMFTDLPESKKPGKKRKRNIPKSGKVSFYRSLQAVGWLLLWQKSGEYFSVETTFTPWYTNKNFLFKLWYLYVLAFTYRLKYYAAWTLSEASCILAGLGYNGYDKETKTIRWDRVQNIDIFAFESAQNIHGALEAWNQNTNKWLKNYVYLRVSKKGKKPGFKSTLFTFLTSAFWHGTRPGYYLSFATGALLQTCGKVYRRNFRPIFIENDGVTPKRTKMFYDIVCYIVTQSALAYLVQPFVILDFKPSLEEWKTVYFYCHVGIGLTLFLFQGPFSAQVTAFFKQYHPVKPKEPRLTDIIKDKQDYESSENMHLGIPSADFEKADLNKIVEELEDFKKDVKDWQQQNGLKTEEKAIKEAYNEFSKDLETLKQKGPNFKKNNKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P721</accession>
    <name>A0A1E3P721_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_77795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040355.1">
        <property type="nucleotide sequence ID" value="XM_019184613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P721"/>
    <dbReference type="GeneID" id="30201859"/>
    <dbReference type="OrthoDB" id="10491621at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="330" mass="38147" checksum="7C4F05CE5C692B7F" modified="2017-01-18" version="1">MFKGQKYQPNTTFQFVNYEQSSSGVFREPKRQKLNIPNTMKFQLANTPPKSTSPDPNLTTISTTVPVSHTHTFVADNLTNSPKTCRKASYTILTTDQKTKLIELIQRFRVYSSKPKLDAWNEVVKSYNAHYNLDDREGLTIQHARIKLNQIITTAENKDPESLSKPERMALDYNKERLEFKNDVNKDLKLDKRYENKNPKLTKVYELILKLQQQLEKARDSNSGSDSDNSFKTELWLLHKRVLDLKTIEEQYQILEQVDYSKEGARVKVSKEMRDELLAKAEDIQLSLDKLLSGLETTEKNGDGEQKESPEVVNENDVVDIELLNQKESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7L0</accession>
    <name>A0A1E3P7L0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Chromatin modification-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_17685</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Component of an histone acetyltransferase complex.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair. Involved in cell cycle progression and meiosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of an histone acetyltransferase complex. Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="16">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the ING family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040628.1">
        <property type="nucleotide sequence ID" value="XM_019180755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7L0"/>
    <dbReference type="STRING" id="683960.A0A1E3P7L0"/>
    <dbReference type="GeneID" id="30198001"/>
    <dbReference type="OrthoDB" id="5411773at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16858">
        <property type="entry name" value="ING_ING3_Yng2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15587">
        <property type="entry name" value="PHD_Yng1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000436">
        <property type="entry name" value="Chromatin modification-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024610">
        <property type="entry name" value="ING_N_histone-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
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        <property type="entry name" value="CHROMATIN MODIFICATION-RELATED PROTEIN YNG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
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    </dbReference>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7 16" id="KW-0156">Chromatin regulator</keyword>
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        <location>
            <begin position="231"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="154"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="163"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
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            <begin position="182"/>
            <end position="197"/>
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        <location>
            <position position="234"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
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        <location>
            <position position="236"/>
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        <ligand>
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        <location>
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        <location>
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            <label>1</label>
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        <location>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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        <location>
            <position position="274"/>
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        <ligand>
            <name>Zn(2+)</name>
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            <label>2</label>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
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        <location>
            <position position="233"/>
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            <position position="256"/>
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        <location>
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        </location>
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        <source>
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    </evidence>
    <sequence length="289" mass="32353" checksum="744BA485D155F96F" modified="2017-01-18" version="1" fragment="single">MDTAAILEQYTQDISNLPAELQHLLSELGTGDSNLYEIRRKILQKDSTIHKFIKQHGSLTKNPKENQLYPKIREDFRKAEQLQREKCITANTALFLTAKHLIKLESDIEKLRSEGLLALDDLDIDSDDELFSSRGASRSTTAGVSLTSNVISNGNGAGHDLPSNVSSVNAAAASTMKKQSRKSTGPKSVTPGASSVASRPLKRHRTEESKTPDPKIETVVLTTVANEEENELYCFCQRVSFGEMVGCDNDDCKYEWFHYECVGLKEPPKGKWYCPDCIERQKQKENKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7M9</accession>
    <name>A0A1E3P7M9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="9" type="ORF">WICANDRAFT_27986</name>
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    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
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        <text evidence="7">Belongs to the protein kinase superfamily.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="C:prospore membrane leading edge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="EnsemblFungi"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="51"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
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            <position position="84"/>
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        <ligand>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
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    </evidence>
    <sequence length="412" mass="47081" checksum="58423BB797EF9A6A" modified="2017-01-18" version="1">MGILKSENTVNLHNNIASQVQPKKPPPQPARFSKPGYVIYEEANFYIDSRFQIQSVLGKGSYGTVCSALDISNKDKPVSMAIKKVSNIFNKEILLKRAVRELKLMRFFKGHKNIVNLYDLDIVYCTPYDGLYCFQELADYDLARVIHSSVQFSEHHIQSFLYQILCGLKYMHSADVIHRDLKPGNILVTIQGTLKICDFGLARGISKEFLKQKPHSITNYVATRWYRAPELLLSKKTYSKSVDMWAVGCIFAELYGRRPIFIGNDQIHQATEIVKILGSPPRDVLLKYGSGLIWELFNSPNPQYKPIPWSQVYPFASKAAHDLLSHLICWHSDSRYNVEEAISDPYLKSVRTPDDEPIARTPFNFEFETLATNLNDYKILIHEEVQQFKIDRLGRLNASQNEGGLSNLLNAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7R3</accession>
    <name>A0A1E3P7R3_WICAA</name>
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        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_28726</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the V-ATPase e1/e2 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040124.1">
        <property type="nucleotide sequence ID" value="XM_019181534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7R3"/>
    <dbReference type="STRING" id="683960.A0A1E3P7R3"/>
    <dbReference type="GeneID" id="30198780"/>
    <dbReference type="OrthoDB" id="1508846at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008389">
        <property type="entry name" value="ATPase_V0-cplx_e1/e2_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12263:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12263">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05493">
        <property type="entry name" value="ATP_synt_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="5"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ60917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="69" mass="7969" checksum="EA339CC0D747BD95" modified="2017-01-18" version="1" fragment="single">YNVIIALFAVVAVSVVFWIFAPKENQTVWRSTVILTLAMCYLMWAITYLAQLHPLVVPRRSDLRPEFAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P821</accession>
    <name>A0A1E3P821_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_59640</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mutarotase converts alpha-aldose to the beta-anomer. It is active on D-glucose, L-arabinose, D-xylose, D-galactose, maltose and lactose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>UDP-alpha-D-glucose = UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:22168"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="5.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">In the C-terminal section; belongs to the aldose epimerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">In the N-terminal section; belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040763.1">
        <property type="nucleotide sequence ID" value="XM_019182846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P821"/>
    <dbReference type="STRING" id="683960.A0A1E3P821"/>
    <dbReference type="GeneID" id="30200092"/>
    <dbReference type="OrthoDB" id="9402762at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052574">
        <property type="term" value="P:UDP-galactose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09019">
        <property type="entry name" value="galactose_mutarotase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05247">
        <property type="entry name" value="UDP_G4E_1_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047215">
        <property type="entry name" value="Galactose_mutarotase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005886">
        <property type="entry name" value="UDP_G4E"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01179">
        <property type="entry name" value="galE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007956">
        <property type="entry name" value="PRK10675.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725">
        <property type="entry name" value="UDP-GLUCOSE 4-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725:SF47">
        <property type="entry name" value="UDP-GLUCOSE 4-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="12">
        <location>
            <begin position="3"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ61556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="654" mass="73311" checksum="05DC71C1007C4BDF" modified="2017-01-18" version="1">MSILVTGGAGYIGSHTVIELIQNNYSVIILDNLSNSSYDAIARIEFIVGRSIPFYNIDLRDHDKLSQLFQSNDIKSVIHFAALKAVGESTKIPLTYYDNNINGTINLLKIMNQFNVKSIVFSSSATVYGDATRFENMIPIPESCPNDPTNPYGQTKYTIEQIIHDLYKSDPTWKAAILRYFNPIGAHPSGLIGEDPLGIPNNLLPYLAQVAIGRREKLSIFGNDYDSHDGTPIRDYIHVVDLAKGHISALNYLEQNQSGLFREWNLGTGKGSTVFDVYNAFCKAVGKNLPYEVVGRRDGDVLNLTADPKRANNELKWFAKLSIQDACADLWKWTIENPFGFNIEGYKWEQFGETRLHHVEIKDFKISIMNYGATVQDLKIGEESLVLGFNDFQSYKSNGNPYFGATIGRYANRISNGEFKLNGKVFKTDVNENSNTLHGGANGFDKQHWLGPIAQISGDSTILQFKLIDQEQSNGFPNQVETIVKFIVGYKSLEIEYQANSNGPTPINLTNHSYFKLGNDIDINLSTKKYLETSNGLPTGEILEIPSQNFKLEDRKFDDCFILNESSSIDTRSNQLIEIFKASTPSHSLTIQSTEPSFQFYTGDGVNIQNFHSRSGFAVEPGRFIDAINSPIYSNQVVLNKDETYGSKTKYIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P827</accession>
    <name>A0A1E3P827_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_59650</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040772.1">
        <property type="nucleotide sequence ID" value="XM_019182848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P827"/>
    <dbReference type="STRING" id="683960.A0A1E3P827"/>
    <dbReference type="GeneID" id="30200094"/>
    <dbReference type="OrthoDB" id="4476201at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF6">
        <property type="entry name" value="GABA-SPECIFIC PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="391"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="425"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="481" mass="52584" checksum="C85B2941CDFC0C8E" modified="2017-01-18" version="1">MMAQENAQVQLAEIGYKQELKRTFNTIQVFGIAFSIMGLLPSIATTMSIGLTSGSIGLVWGWFLGGAFILTIGIGMSELSSSIPTSGGLYYWTHYYGPIKFKNLLSFVIGCSNTIALAGSTCSITYGFAHQVLAAAFLGSNGSFEITDGEVYGVFAAGIFGEISSFNNATYIFGHFENHSDWNDGWTFMQFGLLPAIWSIGAFDSCLHMCEEVKTPSKSVPIGIIGSITVCWILGFLIMIVISACMTQDVESLINTDLGQPLAQVFYDSMGAKWAIAFMSLCAFCQFLMGASILTAISRQVWAFARDDGLPFSSIVKVVNKKLAVPLRAIVFSAIISLLLGLLILAGSIAANALFSIAIVGNYAAWAMPQILRFTSGRELFKPGYFYLGKIFSPLINFISIIFQGFMMILACFPDNKKVKDAKSMNYAVVVNCGVWILSIVYYYAYKKRVYCGPKSNLGEDQLIEAAMLTKEEIEMVDHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8V7</accession>
    <name>A0A1E3P8V7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_77942</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019184637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8V7"/>
    <dbReference type="STRING" id="683960.A0A1E3P8V7"/>
    <dbReference type="GeneID" id="30201883"/>
    <dbReference type="OrthoDB" id="418495at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="term" value="F:glutathione disulfide oxidoreductase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694:SF18">
        <property type="entry name" value="GLUTAREDOXIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="1">
        <location>
            <begin position="21"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="111" mass="12823" checksum="C8FD77590292AC0E" modified="2017-01-18" version="1">MVSANYLKKAQAFIDSNKYFMISKSWCPDCVYAENIWKKYGVSSKVEILELDKLADQKEALELEKAFTEIAGRKWVPTIWFNGSRFGTEQDLKRLESEDATEKEFKNAGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P937</accession>
    <name>A0A1E3P937_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_68045</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid/choline transporter (ACT) (TC 2.A.3.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040669.1">
        <property type="nucleotide sequence ID" value="XM_019184109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P937"/>
    <dbReference type="STRING" id="683960.A0A1E3P937"/>
    <dbReference type="GeneID" id="30201355"/>
    <dbReference type="OrthoDB" id="2417308at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015101">
        <property type="term" value="F:organic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000046">
        <property type="entry name" value="Amino-acid permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF7">
        <property type="entry name" value="CHOLINE TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="156"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="244"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="307"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="397"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="443"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="496"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="522"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="556"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="592"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="51"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="52"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="634" mass="70035" checksum="70D3F90D95C94D7D" modified="2017-01-18" version="1">MAKPSKGIKKKSSKTSSKILKKNSKSTSSKVDKLDSKIDVSEITLLQLNQSNDEKKKSILDHKSLKKDHDKDVETKENEKKNEGDLMKHLKNRLISTLKQNTSSNSISKMSDSEKLKSVNIDADSASVQTGSQMNVDDLKLAEHGYKPELRRNFSIWSLLGVGFGLTNSWFGISASLVAGISSGGPLLIVYGIIVVACMSSCIGISLSELTSAFPENSGGQYYWTFQLAPKQYKRFWAYMCGSYAWFGAIFTSASTTLSVASAIVGMYVLGTGDPNREVQRWEVFVCFEIMNAFLIIFNVWDKPLPYISSVALWTSISSFVVITITVLICSRGHYQTASFVFVEFNNATGWSSKAIAFIIGLQSPNWAFSCLDASCHMTEEIASPEREIPKAIMSTVAIGFATSLIYSISMFFSITDLSAILASNTGVPILDIFDQALKNRHGALFLESLVILTGFGCTIAAQTWQARLCWSFSRDQGLPGSRYWSRVNSKTNLPINANLISVACSALIGCIYMGSTTAYNAMVTGCITFLLLSYAIPICCLLYKGRSNIKHGPFWLGKFGFAANILTLCWTVFAIVFFCFPYTIPVTKGDMNYASVVLVGFTIYAIIYWFARGNKVFAKNHDIEKEVEQDLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9K0</accession>
    <name>A0A1E3P9K0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_82062</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041101.1">
        <property type="nucleotide sequence ID" value="XM_019185326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9K0"/>
    <dbReference type="GeneID" id="30202572"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="59" mass="6822" checksum="C2E7BFA1136A2DA9" modified="2017-01-18" version="1">MESREINIWLICCLDSLPRIIHVVFLTERVLFKVSTVVNISNSPWITDALKKTFAIAFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAJ2</accession>
    <name>A0A1E3PAJ2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Luciferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_66702</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the NtaA/SnaA/DszA monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041646.1">
        <property type="nucleotide sequence ID" value="XM_019184050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAJ2"/>
    <dbReference type="STRING" id="683960.A0A1E3PAJ2"/>
    <dbReference type="GeneID" id="30201296"/>
    <dbReference type="OrthoDB" id="5561043at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051260">
        <property type="entry name" value="Diverse_substr_monoxygenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016215">
        <property type="entry name" value="NTA_MOA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03860">
        <property type="entry name" value="FMN_nitrolo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011">
        <property type="entry name" value="ALKANESULFONATE MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011:SF16">
        <property type="entry name" value="C2H2 FINGER DOMAIN TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000337">
        <property type="entry name" value="NTA_MOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="6">
        <location>
            <begin position="49"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="503" mass="56960" checksum="DABF173374ED0EE1" modified="2017-01-18" version="1">MTTGEHISKKQKTEQKREPLILNFFEALTPVLHAPGQWRNPRDKSREYYKAEYWVKLAQLAERGKLHGLFIGDSLAIYGGYKGPFNFSEAAKSGNNFPKCDPTAYLAAIAISTKNVSVGLTCSTLSEHPYHFARRIATLDHISGGRVGWNVVTSLLPSAGRQLTDDNILPEKTLRYEKTDEYLQVFYELLLSSWRDDALVYDKEKGIFSDPEGIREINFDGKFFRVPGPQITEPSPQRIPLIIQAGSSPKGQLLGAKHGEVIFLIGDTPEDAKEKIDTVKKLAKEEYGRNPDHLKFLTNIRVVVGKTHEEAVKKAEEIESYKDLDGTLVQLGGGGLDLSKWDWDEDLTNVDDPAVKGLANSFLKKPFHKEGEIVTRRYIAANFFHSNYFIGTPEEVADQIEDYVERSTINGFNFSNVVFPETFEDIVDLLIPELQKRGLAQKEYAVEGGTYREQVYRTKGHTFVPEDHYAYGLRWKAGVSKEEFEKNLEALKEKQKELKITSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAK0</accession>
    <name>A0A1E3PAK0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_76617</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041656.1">
        <property type="nucleotide sequence ID" value="XM_019184420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAK0"/>
    <dbReference type="STRING" id="683960.A0A1E3PAK0"/>
    <dbReference type="GeneID" id="30201666"/>
    <dbReference type="OrthoDB" id="159449at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="143"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="507" mass="58802" checksum="5F85F2F4AD6A2CE8" modified="2017-01-18" version="1">MDSTTFQQLLELESNLSKAQQFLNTSNQTALLLSHLENLNNHSTAHTGFDGVEIKDIESLLQQTGSFDLAISCSSCNQLIYENDECAKNQIHKDNLQPCCMKMLKNLKQDDELEITFWWFFINNFSNCILTLPNYTRFMLTNGIPTSLRDRIWGLLTFSNNSKGENDEYLLNLYESLNKDISPDIRIITKDVNRTYPQLSMFTEFETKSKFEKILNAYSIFDSDMGYCQGLQFIVAPMLFHFDKDDFKTFNSLVKFFEINKLRNIYDNQMSGLNLWFYQFEEIFKHELPELSTYLIDTLNVDLKVFLSQWFLSFYSITIPFNFLIRVFDIMFLEGVKETLLRVGIVILSKNSKLLQSIDESELVYQHLLSENCWGVFQNNVDSFINEIMNLNIEQYTEENLQQLSTNYSQSTTKEEQDISPNSFISKMFTNLKYNAESILSSPIDSISSSKSSLFSINSESESSNTSIFESDFDLVESLYKMCLEKGLDGPILDKVKDRLYQTTTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAT0</accession>
    <name>A0A1E3PAT0_WICAA</name>
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        <recommendedName>
            <fullName evidence="4">Protein STB3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_103301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ62526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:cellular response to glucose stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000432">
        <property type="term" value="P:positive regulation of transcription from RNA polymerase II promoter by glucose"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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            <end position="65"/>
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    </feature>
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            <begin position="193"/>
            <end position="350"/>
        </location>
    </feature>
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            <begin position="27"/>
            <end position="37"/>
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            <end position="63"/>
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    </feature>
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            <end position="350"/>
        </location>
    </feature>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="ODQ62526.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="391" mass="43637" checksum="757F6A2420F0F63B" modified="2017-01-18" version="1">MSLKSTTSPDSLAPTSPFSNYSNLSTSSISNHSTNVSPNFTTTTHKEKKSAQKHQTQLSTSSPAGIAVASKVTPERLAHLLLETGPLAIRHITSNLAKQIQGFEKLSLSKQRRLIMSSLDQGFEEESIIFEKIGWGQWSAKHVEPDNFIKERNAIRIANAKVKDGEHNNATARRESITKNKYEIDHHDKFLKKHGSNSTLTSKTAGKSTNSMMMDDDYAIASDDDEEEEEDLANGASSTSDFYDDDDEAENGVFKFDDEEQIPPLKLNSRLVSPPYQTRSSRSSFSRGISKPKFKSVRSNSQTPTSPELLYYQKIEKSKNSTAINRRHSSIRSTLNNDQNDESDTDEEDWQSIGALKLRKNSVAPPEISIPEHLQKDEQDAAFALVNLRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAX0</accession>
    <name>A0A1E3PAX0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein BOI2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_76734</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ62565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019184441.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3PAX0"/>
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    <dbReference type="OrthoDB" id="73680at2759"/>
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    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001881">
        <property type="term" value="P:receptor recycling"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAM_BOI-like_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3_BOI"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Boi1/2_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045188">
        <property type="entry name" value="Boi1/Boi2-like"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22902:SF27">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY A MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="domain" description="PH" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="805"/>
            <end position="924"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="102"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="359"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="398"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="515"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="553"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="586"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="616"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="811"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="837"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="857"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="890"/>
            <end position="901"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="950" mass="104308" checksum="3941190E5E02FB06" modified="2017-01-18" version="1">MSSNELRQQLPSIKTTNLEENATGAIYQVIKEFNARLEDELTLKVGDKIEVISDDKEYNDGWYMGKSLSTGSVGLYPKSFTQLIPSLSRPTLLRSRSRRIASPNSNNQSPVSLPTSSAANTPATVKTQRQFIPEEATETTKETIDHKKPNSVTRALSDIDKALEELRSEASPNPNNDVNSSGFGAGANGKDKHRSFSIQSEDSLNPELVESWSPEQVTAYFSYLGFDVNSAGQFARHKITGAILLELELAYLKELDISSFGTRFEMFKEIEALKELAKQKEIDQSSAEDSPYRLLAPAELHSSRNVSKTNQLDDDYDVFTRPLGHARKKSQSLDDIPEVTRQQQQQDNRHPLRPLSVHPPSSQVPDLPVIQQQFASPRRAPNPPSYPSPIQLKEDNNSNHLTPTVNRTSRNSYIELSHSRKPSYEVNTAYQVPRSGNHSRNNSANFKSQSVSDLLSTTHLDNGRPASSIYMPEPQHSRTSSANFRRGHIRGASDISVANSTKASKEERAHRRHSSVFSFLSNANFNNGGSISPTRKSPTRRNSVINTDGAVSPSRNGFRSQDAQMTPQLQSTPQLQAPDKKRRSVSAKEADKEESPAKSPAGKRSVSEAVRAKTLRNISSSTTLRSRNTKKDTSAFMEGIRNTNPADSIKTADCFGWMSKRGGIAVGTWKQRYFTLHGTRLSYFTSLSDTRERGLIDITSHKVLPAKDDDKFVSLYAASTGHGKFCFKLVPPAPGSRKGLTFTQPKIHYFAVESKEEMRTWMAALIKATIDIDESVPILSSCATPTVSLQKAQALLVQARENAKKREDAYRQTQEQLNQPQHQAGKLAPNGSLNDVSGTSSISPDSGSFDSNIPAHSPNTTATTSEMTPIMQNDNGFSSPYNIASGFISPKSTPSSANPSPIDTEFKSKPYSAGAIRHPSQRVASSASAAAAAAVAHAGKQRSSEPSNRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAX5</accession>
    <name>A0A1E3PAX5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA (guanine(10)-N(2))-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.214</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_60630</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM11 methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.214" evidence="9"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041783.1">
        <property type="nucleotide sequence ID" value="XM_019183028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAX5"/>
    <dbReference type="STRING" id="683960.A0A1E3PAX5"/>
    <dbReference type="GeneID" id="30200274"/>
    <dbReference type="OrthoDB" id="296065at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043528">
        <property type="term" value="C:tRNA (m2G10) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160102">
        <property type="term" value="F:tRNA (guanine(10)-N2)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000260">
        <property type="entry name" value="RNA methylase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016691">
        <property type="entry name" value="TRMT11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059073">
        <property type="entry name" value="TRMT11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370">
        <property type="entry name" value="RNA METHYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370:SF3">
        <property type="entry name" value="TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25904">
        <property type="entry name" value="Tmrp11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017259">
        <property type="entry name" value="tRNA_mtfrase_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51627">
        <property type="entry name" value="SAM_MT_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 10" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA (guanine(10)-N(2))-methyltransferase TRMT11 N-terminal" evidence="12">
        <location>
            <begin position="3"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA large subunit methyltransferase K/L-like methyltransferase" evidence="11">
        <location>
            <begin position="185"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ62576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="440" mass="50445" checksum="E209574F41AAEA13" modified="2017-01-18" version="1">MAKEYLVFLAQQHPTFRVPELESLGDIHGIKVDFSGHDENTPFMIITLDNDQDAKNLISRAVLAKGIYELWGIGDTEEELHEDVKRRSSDKFELYKECSFKFDVIGYMGKRNNSEKIKLIESFKYVEFEGPIRMKNPDETFTVLEHYVPPTENDSAVIPTKLYFGRQIELSARERGILDKYDLRKRRYIGTTTFEAELALVSCNLGQVDTGKIMYDPFVGTGSFLVAGANYGALTVGSDIDGRLIKGKGNNTIRANFKQYNTSSLFMDVLTMDFTHNAFRSNMKIDTIVCDPPYGVREGLKVLGAKNPDRFAGKEEVEIDGKKAFLRKDYIPTKKPYELDALLCDLLQFGVDRLPIGGRLCFWMPTANDEYVPTIIPKHRNLELKYHLVQEFNKWSRRLLVYINRGDDYNGEDNMGATSIPEFRKKYFTTPSREQKNQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAY2</accession>
    <name>A0A1E3PAY2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PQ-loop repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_38748</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041657.1">
        <property type="nucleotide sequence ID" value="XM_019182540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAY2"/>
    <dbReference type="GeneID" id="30199786"/>
    <dbReference type="OrthoDB" id="407617at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF37">
        <property type="entry name" value="PQ-LOOP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="191"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="263" mass="29380" checksum="5F222CB2423A03B1" modified="2017-01-18" version="1">MTSETPVANILGTIGTVCWCIQMIPQIIHNYRNKNCEGLQPLMLFLWTASGVPFSIFFVSRDASIPIQIQPQCFTFLCLVTWAQTLHYPPVSQSKKKTILYVGLFLIISIALEVGFIIPLKRLTAKNIMWPSLIFGILASILLAVGLLPPYFELAKRQGRVVGINFVFLSLDFSGALFSALSVAFAREVDVLGIVLYCIVGFMELGIFSSHLIWYLRIGRKMKKDDNRESEDELATSITEVDDLKKAESNETTKSCDTADTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBQ4</accession>
    <name>A0A1E3PBQ4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyltransferase family 32 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_25303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041599.1">
        <property type="nucleotide sequence ID" value="XM_019181126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBQ4"/>
    <dbReference type="STRING" id="683960.A0A1E3PBQ4"/>
    <dbReference type="GeneID" id="30198372"/>
    <dbReference type="OrthoDB" id="409543at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039367">
        <property type="entry name" value="Och1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834:SF1">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="406" mass="47450" checksum="1803ABB9DBAAD219" modified="2017-01-18" version="1">MLIIGALTLYTYVLFNTEYIYPSIINNTLKSINLLNDQAEQVLGKTSDPIAALQSTIASTLQNNDDSTFEDYVQQYAPEDLRSRLAKKYPYSRESTIPNEIWQTWKEPIEKISNTNLLKYIDSWESQKDYNYHLITDDKIDSTITEMYVEFPEILDTLQKLPKNILKFDFLRYLILFAKGGVYSDIDTINLKSLSQWADTPQECSNYFDQSPTVNNNIGFVVGIEGDYDRADWKYKMARRVQFCQWTFKSKVGHPILRELIYSIVDLTLNNYNERLNHVKIGSDYHTLHSIYTILNWTGPGIFTDTLFDHLNKIFKNSKIQNKNPIPNIKHPTFEYYHKERNEIDVEGGKYVGWSNFTKISSPILYDDVLVLPINSFNAFNEDLEIKPDDKLTYVKHDFRGTWKSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBS4</accession>
    <name>A0A1E3PBS4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Long chronological lifespan protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_25071</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LCL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041957.1">
        <property type="nucleotide sequence ID" value="XM_019181095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBS4"/>
    <dbReference type="GeneID" id="30198341"/>
    <dbReference type="OrthoDB" id="2234316at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23996">
        <property type="entry name" value="LCL2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034543">
        <property type="entry name" value="LCL2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38425">
        <property type="entry name" value="LONG CHRONOLOGICAL LIFESPAN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38425:SF1">
        <property type="entry name" value="LONG CHRONOLOGICAL LIFESPAN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133771" description="Long chronological lifespan protein 2" evidence="4">
        <location>
            <begin position="18"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="127" mass="14482" checksum="14AEF26D12412EDA" modified="2017-01-18" version="1">MVIQFLITLLLAFQANAQFFNFFGGQHQQQAQKQSYEDEFLNNKCGDYLCPDTQECVKTKLDCPCPFPKSQLKCVLPNKQNFVCISKPATNDERLQQLYDDPVKGPKAKTDGLRDCGWVEQAWNGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBT5</accession>
    <name>A0A1E3PBT5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HPP transmembrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_76590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041629.1">
        <property type="nucleotide sequence ID" value="XM_019184415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBT5"/>
    <dbReference type="STRING" id="683960.A0A1E3PBT5"/>
    <dbReference type="GeneID" id="30201661"/>
    <dbReference type="OrthoDB" id="2016548at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007065">
        <property type="entry name" value="HPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058581">
        <property type="entry name" value="TM_HPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33741">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN DDB_G0269096-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33741:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN DDB_G0269096-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04982">
        <property type="entry name" value="TM_HPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="HPP transmembrane region" evidence="2">
        <location>
            <begin position="49"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="295" mass="33185" checksum="362CD31D95F6F516" modified="2017-01-18" version="1">MPFSIDNYLNPYYPSSIIPQIPSKLVQRVLGHHKDLKSKDSSILLYLDILIGSFVSILLIEGVFKHSHVFIQHHSPIIIASYGASAILLFNANTAPLSQPRNIIMGHFLSALVGVILQKLFELSSNGRDHLYIGGALSVGISSVVMSIFNCVHPPSGASALMPLVDEGIREMGWWYLPAHLISSVLMVCVACITNNILRKYPQYWWTSYKKEAIKDDEEMQEIKKIEPERSNNETIAVKSNDKQLKTSNFGKIEITSHELNIPDELALTFEELDLLHGIRNRLASHHHNKNQPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBV3</accession>
    <name>A0A1E3PBV3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-deoxy-7-phosphoheptulonate synthase</fullName>
            <ecNumber evidence="4">2.5.1.54</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">3-deoxy-D-arabino-heptulosonate 7-phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">DAHP synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Phospho-2-keto-3-deoxyheptonate aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_60500</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-I DAHP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="4"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041649.1">
        <property type="nucleotide sequence ID" value="XM_019183005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBV3"/>
    <dbReference type="STRING" id="683960.A0A1E3PBV3"/>
    <dbReference type="GeneID" id="30200251"/>
    <dbReference type="OrthoDB" id="3980943at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006219">
        <property type="entry name" value="DAHP_synth_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE DAHP SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225:SF12">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE, TYROSINE-INHIBITED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP synthetase I/KDSA" evidence="12">
        <location>
            <begin position="152"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ62442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="460" mass="51169" checksum="2A315BEACFAEE396" modified="2017-01-18" version="1">MMKESSKFSTVCSSQRFKTLRWDSLAEAVGDLSTRSDECLNERDQVALTQPDVVKEFLCPVSSTLKSSIIQFRESFSNDIHNPIPSSRFIITGPREVNDHHQAIACSYWLLNKSNISTSDSIINTKKLPDKVQKLYSSNNPPLKNLKLAMNLNISGEIKTSNSSYMTQTVFHGVFACRVLLMTLASQIPIIGEIGDPIGLEYFKDLFGCGMVGPGTIESQVHRELASGVPFSIGFSASSFRSVFSSDILNHKVQKAIDAILASADPHYFLSVTKLGNVAITSSSGNKDSFLVIPIQYNSIVLEFNYVVGVVDGLLEGVHTPKIVLDVGKVSSVNLREVLELLEQILRALTLRNFIIGFIIDSGDRYVPDNIEGEPTTHQITQDDYESLDEKENFSVMSENIGIKRPSMRNKIFTSVKSSYSKIRSTLTDKRNSDIGQYQDLIYADILINEIEQLIENLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBZ5</accession>
    <name>A0A1E3PBZ5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alkyl hydroperoxide reductase subunit C/ Thiol specific antioxidant domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_60876</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042037.1">
        <property type="nucleotide sequence ID" value="XM_019183072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBZ5"/>
    <dbReference type="GeneID" id="30200318"/>
    <dbReference type="OrthoDB" id="3997115at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="189" mass="21780" checksum="9A4C1CAB60482E7A" modified="2017-01-18" version="1">MKRRYSNCEDCLPSPEYSEPEYPILGLNTKKTRITQTSHEFIEAIDDSILSFINRDGFISQKKFIVKDKPTNLVLVFFGVIDDFLEPISNAHDKLSQIFNAEIIGISSNISRNHHIFPLINSSKIPKKFNVLDPVGGGVYPRDVVIIFDKTGRRRVEIPVKLYNRYLLDRPITDVLMDYLEELESESML</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC12</accession>
    <name>A0A1E3PC12_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_82845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042153.1">
        <property type="nucleotide sequence ID" value="XM_019185451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC12"/>
    <dbReference type="GeneID" id="30202697"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="53" mass="6163" checksum="4F61FBAE11AF0429" modified="2017-01-18" version="1">MIVVYNGSRSRDWIFSLPCCVRPFEELNDQTRKNKERKFNVNESEGPEEGGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC76</accession>
    <name>A0A1E3PC76_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_89371</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042223.1">
        <property type="nucleotide sequence ID" value="XM_019186180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC76"/>
    <dbReference type="STRING" id="683960.A0A1E3PC76"/>
    <dbReference type="GeneID" id="30203426"/>
    <dbReference type="OrthoDB" id="9626941at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013945">
        <property type="entry name" value="Pkr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28251">
        <property type="entry name" value="V-TYPE ATPASE ASSEMBLY FACTOR PKR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28251:SF1">
        <property type="entry name" value="V-TYPE ATPASE ASSEMBLY FACTOR PKR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08636">
        <property type="entry name" value="Pkr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ63016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="119" mass="13433" checksum="DB5CA82E67E3B809" modified="2017-01-18" version="1">MDFVNELWKSVLTPGTSPALILATHLTFASLVITLSFFALYSKSIHLINLLVISILLWITLTWFINEINSLEKSDLKDNNELAEEKTDTETKNQAVESKSSSSEPKKVIQPVQRKSKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCA2</accession>
    <name>A0A1E3PCA2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase</fullName>
            <ecNumber evidence="6">2.1.3.2</ecNumber>
            <ecNumber evidence="5">3.5.1.2</ecNumber>
            <ecNumber evidence="20">6.3.4.16</ecNumber>
            <ecNumber evidence="4">6.3.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="ORF">WICANDRAFT_61036</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="32">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="25">Multifunctional protein that encodes the first 2 enzymatic activities of the de novo pyrimidine pathway: carbamoylphosphate synthetase (CPSase; EC 6.3.5.5) and aspartate transcarbamylase (ATCase; EC 2.1.3.2). The CPSase-function is accomplished in 2 steps, by a glutamine-dependent amidotransferase activity (GATase) that binds and cleaves glutamine to produce ammonia, followed by an ammonium-dependent carbamoyl phosphate synthetase, which reacts with the ammonia, hydrogencarbonate and ATP to form carbamoyl phosphate. The endogenously produced carbamoyl phosphate is sequestered and channeled to the ATCase active site. ATCase then catalyzes the formation of carbamoyl-L-aspartate from L-aspartate and carbamoyl phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">In the 2nd section; belongs to the CarB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">In the 3rd section; belongs to the metallo-dependent hydrolases superfamily. DHOase family. CAD subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">In the C-terminal section; belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">In the N-terminal section; belongs to the CarA family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="6"/>
    <dbReference type="EC" id="3.5.1.2" evidence="5"/>
    <dbReference type="EC" id="6.3.4.16" evidence="20"/>
    <dbReference type="EC" id="6.3.5.5" evidence="4"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042198.1">
        <property type="nucleotide sequence ID" value="XM_019183100.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3PCA2"/>
    <dbReference type="GeneID" id="30200346"/>
    <dbReference type="OrthoDB" id="1924069at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005951">
        <property type="term" value="C:carbamoyl-phosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004087">
        <property type="term" value="F:carbamoyl-phosphate synthase (ammonia) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01423">
        <property type="entry name" value="MGS_CPS_I_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000002">
        <property type="entry name" value="Aspartate carbamoyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000025">
        <property type="entry name" value="Bifunctional pyrimidine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000005">
        <property type="entry name" value="CAD protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000011">
        <property type="entry name" value="CAD protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000004">
        <property type="entry name" value="Carbamoyl-phosphate synthase (glutamine-hydrolyzing)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1030.10:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000036">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1380:FF:000009">
        <property type="entry name" value="Carbamoyl-phosphate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="entry name" value="Carbamoyl-phosphate synthase small subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="entry name" value="Carbamoyl-phosphate synthetase, large subunit oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CPSase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CPSase_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058047">
        <property type="entry name" value="CPSase_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002032">
        <property type="entry name" value="PRK00856.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003671">
        <property type="entry name" value="PRK05294.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009455">
        <property type="entry name" value="PRK12815.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009475">
        <property type="entry name" value="PRK12838.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF5">
        <property type="entry name" value="CAD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25596">
        <property type="entry name" value="CPSase_L_D1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00101">
        <property type="entry name" value="ATCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="Carbamoyl phosphate synthetase, large subunit connection domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="Carbamoyl phosphate synthetase, small subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-grasp" evidence="29">
        <location>
            <begin position="558"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="29">
        <location>
            <begin position="1096"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="30">
        <location>
            <begin position="1353"/>
            <end position="1504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="1877"/>
            <end position="1903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="28">
        <location>
            <begin position="1877"/>
            <end position="1887"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00043968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00043984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00044063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00058513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00081752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="EMBL" id="ODQ62991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="2221" mass="245078" checksum="83E92E207F4ED8F0" modified="2017-01-18" version="1">MASLTPSAPISPPMESTGNRLMALELKDGSVLEGYSFGSEVPTAGELVFQTGMVGYPESITDPSYEGQILVITFPLVGNYGVPDRELKDDDVPELPKYFESSKIHIAGLVVAHYTEEYSHFLAKSSLGQWLKEQNIPAIYGVDTRALTKHLRESGSMLGRIGVQNDKASVEEAASENWKSHFDIPEWDDPNVKNLVAKVSTPKPILYTPSAEDAIKGPDGKTLRILAVDVGMKFNQIRCFVRRGVELKVVPWDHDITSEEFDGLFISNGPGDPSVMDSTVEQLSKILASKKTPVFGICLGHQLLARASGASTIKLKFGNRGHNIPCTSTISGRCYITSQNHGYAVDTTTLASGWKELFVNANDGSNEGIYHTELPYFSVQFHPESTPGPRDTEFLFDVFIKSVLEHKTSKTLKAVEFPGGKIEDNLAKSPRLHPKKVLVLGSGGLSIGQAGEFDYSGSQAIKALKEEGIYTILINPNIATIQTSKGLADKVYFLPVTADFVRKVIKHEKPDAIYATFGGQTALSVGIELKDEFESLGVQVLGTPIDTVITTEDRELFARAMDEIGEKCAKSQSASSVDEALAAVRDIGFPVIVRAAYALGGLGSGFANNEEELIDLCNKAFAASPQVLVERSMKGWKEIEYEVVRDAFDNCITVCNMENFDPLGIHTGDSIVVAPSQTLSDEDYNMLRTTAVNVIRHLGVVGECNIQYALNPDSKEYCIIEVNARLSRSSALASKATGYPLAFIAAKLGLNIPLNEVKNSVTKVTCACFEPSLDYVVVKIPRWDLKKFTRVSTLLSSSMKSVGEVMSIGRTFEEAIQKAIRSVDYHNLGFNQTEALMSIDIDSELQTPSDQRLFAIANALHSGYSVDKVWRLTNIDKWFLNKLNGLINFAQHIESFGSIENLTPLVIREAKQLGFEDRQIAKFVGSNELAVRRVRKEFGIIPFVKQIDTVAAEFPAFTNYLYITYNADSHDVKFDDHGVMVLGSGVYRIGSSVEFDWCAVTAVRTLRANGTKTIMVNYNPETVSTDYDEADRLYFETINLERILDIYEIEQSSGVVISMGGQTSNNIALPLHRNNVKILGTSPEMIDSAENRYKFSRMLDRIGVDQPAWKELTSIDEAEAFADDVGYPVLVRPSYVLSGAAMNTVYTKDDLASYLDQAVEVSRDYPVVITKYIENAKEIEMDAVAKDGNLIMHVVSEHVENAGVHSGDATLIVPPQDLDPETVKRIVVATAKIGQALNITGPYNIQFIAKDNDIKVIECNVRASRSYPFISKVVGTNLIEMATKAIMGFPVEPYPLNKLPEDYCGVKVPQFSFSRLSGADPILGVEMASTGEVATFGHNKYEAYLKSLISTGFHLPKKNILFSIGSYKEKLELLPSIRRLSELGYNLFATAGTADFIQEHGIPVQYLEILNDEETQRSEYSLTQHLANNLIDLYINLPSSNRYRRPANYVSKGYRTRRMAVDYAVPLVTNVKNAKLLIEALARNISLDVSNIDAQTSHRTVSIPGLVNISSFVPSFETSQDFEQATSASLVSGFTYNQILPISTEGLTISDAKSLNEVTDLLADSGYTDYTLSTAAIKGNEAQVSQVANSVGSLFLSFNEFSNGHVSDVSGHFASWPESKPIITDAKSTNLASLLLLASLNNRKIHVTGVQSKSDLEIIRLVKEKSAHVTVDVNIYSLFVSQEDYPEATFLPTKEDQEALWAGLEHIDAFSVGILPSVLAKFLGKPVTAGLGITDALPLLFSAVTDGRLTIEDIVDRLHTKPVEIFSLPEQDSLVELDLDRLASTLHSANIWSPFEGKLKGGVERVHLHGQTVCLEGELVINQALGVDNSAFKKRMGSIVQTPSFNNDVALAQSPQVRRARFSFSADAQHRPSFVEEIPSPEKETRGTELVSSRPPRELSPPGAIATHIRGNNPFLRRSILSVNQFGRSDLHALFSVAQEMRLAVERKGVLDLLQGRLLTTMFYEPSTRTSSSFDAAMQRLGGRTIAVSAASSSVKKGETLQDTIRSLASYSDAIALRHPDEESADIAAKFSPVPILNGGNGSREHPTQAFLDLFTIREELGTVNGITVTFMGDLKYGRPVHSLCRLLRHYQVRVNLVSPKELRLPEHLRQELIDANMLASESEKLTKDIIAKSDILYCTRVQQERFEDQEQYERLKDSYIVDNKILSYAKQHMAVMHPLPRVNEIREEVDFDQRAAYFRQMRYGLFVRMALLAMVIGVDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCC3</accession>
    <name>A0A1E3PCC3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flavoprotein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_89369</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042218.1">
        <property type="nucleotide sequence ID" value="XM_019186179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCC3"/>
    <dbReference type="STRING" id="683960.A0A1E3PCC3"/>
    <dbReference type="GeneID" id="30203425"/>
    <dbReference type="OrthoDB" id="1532798at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990143">
        <property type="term" value="C:CoA-synthesizing protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF6">
        <property type="entry name" value="PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="4">
        <location>
            <begin position="315"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="143"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="246"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="504"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="23"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="143"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
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        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="504"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="521"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ63011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="583" mass="64128" checksum="4974BAA6F7A86B32" modified="2017-01-18" version="1">MPDQSEQQQQQHSGVASQDSDPRSPQAQNKPALSILNKSNSSPVKSQIANENQIKRSNNLKLNTTSSGPANSSTISTPDMITPTRPSVVPTQFPTVASDVPGSNKYGSVSFTVPDQTEKSHRGSISARPSSVQINTLGQPIQTQKVTQPVQQHHPTSATTIKTPTATTTTNETLSKVSSPSQGNVSRQPSITNSIPQVVSSRNPSISSISNPSANSSRQNSNTNTDFINFTANTFSNYHNYPQHNITRVKSPESINRNQSTSPTSHERGPSPPVPQPIAIPKKPKEKQVKAVIGTPTTQVPFYEFFQKQDDQKIHILIGATGSVATIKVPLIIDKLYKTFGASKVSIQLVVTKAAEYFLKGLKLNSEVKIWRDEEEWFGYKKIGDPVLHTELRKWADILLISPLSANTLAKISNGICDNLLTAIIRSWNPATPILVAPAMNTFMYTHPVTKKHLNSIQEDSPWIEILKPVEKVLVCGDIGMGGMREWSDIVEILVKKLKEIQKSRADDDSKKAQTENGENNGEDEDEDNEDEDDDEDDEDDDDDDDDDDDDDDDDDEDEDAVQELPNDETLQGLRDNGTSISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCS7</accession>
    <name>A0A1E3PCS7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_99295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019186686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCS7"/>
    <dbReference type="GeneID" id="30203932"/>
    <dbReference type="OrthoDB" id="3993678at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ63014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="222" mass="24556" checksum="792B13889C3ADF23" modified="2017-01-18" version="1" fragment="single">MIQELWGNPYLEARDPRAMSLQGNKNPYGPDPRAMSLQGRNPYQEMNNDPRARFMNGQPRPNLTQPRTNSLTNQPYQQGSPRAYSLTTNPYQQVNNPMARTPINNGELQAPNQHFIQSGANNSPKFGSQPSLVPNRNSGSGIPQSLQQKRQVPLQNGFQPPKPQNMANSFGGYNPSTPTIEEAPSLQDTESQYSEPPSATTTVASRKPPPVSGDFSHHYLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUJ6</accession>
    <name>A0A1E3NUJ6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial</fullName>
            <shortName evidence="8">Glu-AdT subunit B</shortName>
            <ecNumber evidence="8">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PET112</name>
        <name evidence="10" type="ORF">WICANDRAFT_59353</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Subunit of the heterotrimeric GatFAB amidotransferase (AdT) complex, composed of A, B and F subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="8"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036022.1">
        <property type="nucleotide sequence ID" value="XM_019182837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUJ6"/>
    <dbReference type="STRING" id="683960.A0A1E3NUJ6"/>
    <dbReference type="GeneID" id="30200083"/>
    <dbReference type="OrthoDB" id="1722066at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00121">
        <property type="entry name" value="GatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004413">
        <property type="entry name" value="GatB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00133">
        <property type="entry name" value="gatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004012">
        <property type="entry name" value="PRK05477.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="GLUTAMYL-TRNA GLN AMIDOTRANSFERASE SUBUNIT B MITOCHONDRIAL AND PROKARYOTIC PET112-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659:SF0">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="GatB/YqeY motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Asn/Gln amidotransferase" evidence="9">
        <location>
            <begin position="348"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ56815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="506" mass="57587" checksum="AA952E0C42DFD2EC" modified="2017-01-18" version="1">MLRCYRSFSTSGIIRKKYQLTPGFDLKCGLEIHSQLDTTHKLFSLSKTSFQSSPNSKISYFDASLPGTQPTLNPEPLLFALKAAKALNSDINTNSTFDRKHYFYGDQPMGYQITQHYQPYAKNGFLKLLPHDGVQKEKIINIEQIQIEQDTGKSVYTEFDNQAFSKIDLNRTNTPLIELVTKPDFTNPEEIRIFIKKFQNLVRHLKICTGDLETGAMRVDVNISINNGHRVELKNLSTTSAVIQAVKYEYQRQIDLVKSGESVVQETRGWNGAKTVRLRTKEAALDYRYMPDPELASICLEDDIETKLVLPKLPDALLDELRNDPYNLSVKDATILTHDLQLLDYYFALFGKTVLGDVSVESKLPGNWLIHELLGGFTKLNLPFDQNLIPVDKLSELLIAIQLEKITKSSAKLLLQHLMNNPQDLETPILNLIQEFDLGKQDTVDLNEIDTIVQDVMTQHENIVQDLQNGKKLNSIKFLIGQCMRQSQGKIKPNIFENRLKEIIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV20</accession>
    <name>A0A1E3NV20_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Secreted protein CSS2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_107313</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036152.1">
        <property type="nucleotide sequence ID" value="XM_019180498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV20"/>
    <dbReference type="GeneID" id="30197744"/>
    <dbReference type="OrthoDB" id="5059029at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046624">
        <property type="entry name" value="CSS2_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20521">
        <property type="entry name" value="DUF6736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133534" description="Secreted protein CSS2 C-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Secreted protein CSS2 C-terminal" evidence="2">
        <location>
            <begin position="68"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ56945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="221" mass="23663" checksum="31E4E250D77AD291" modified="2017-01-18" version="1">MFKILFQLLAISAAFVLTAPVNNSTTGISKLNSQQYNVVPFGHVDLDDYLSNSTTFANHTYLSAPGSQNGLERRGSVHACQGSFTKKLCITGSNAANLVGALCHSISVALEIQSGNHNCADTGVHSVSDQTQGTVYYKYKTTGKGCTTTAEPGTIEGALHKFIKEKNQDKICDFVCIRLTHGGNWSGYVGLGTSQDAVKYSRCDHEIGSWSACVNGDKGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVI6</accession>
    <name>A0A1E3NVI6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_81338</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036322.1">
        <property type="nucleotide sequence ID" value="XM_019185218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVI6"/>
    <dbReference type="STRING" id="683960.A0A1E3NVI6"/>
    <dbReference type="GeneID" id="30202464"/>
    <dbReference type="OrthoDB" id="79687at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14011">
        <property type="entry name" value="PK_SCY1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF6">
        <property type="entry name" value="SCY1-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="622"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="692"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="636"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="696"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="786" mass="89188" checksum="4165F4A69907A3F9" modified="2017-01-18" version="1">MFKWKTGIQGNYSIASNPSFHSDLWAIYPARHKSTDRQASVFIFDKKKFESLLLKNNIIKKHDKQVINDAYEILRNQVSNLAKFRHPNILTLIEPLEDHKSNFIFVTEYVTACLNSVPNDLDDVVIQKGLLQISNALNFLHCTAKFIHLSIQPSSIFINDNSDWKLSGFGHIQPIELEEFFIPQYDPRMPNFVNINLNFTAPELVYDNKIYPASDIFSLGCVIHYLYNHGEKNLLNCSNSTSYYKDEFNKFEKLLKQNNLKSFFKKVPDSLLEILPMMLSRYPNQRLTIQDFLSSGYFDNPLIKTMIFLDEFPTKNDDEKLIFLKSLVNLLPLFPTSILQKKIMPILLELLNNGPKLITSLIQVSLTIVLLIGDTLSQLTFHDKVFSTISNESLISTSEAQIVLLENLKILQKKIKNDEFKKLLIKLSEKTLDLKANSQIQTKSLENIDFFLQSIDFPTIKNNIFPKICSIFAKTTSLTVKVQTIYSFNTLIERKGIDKFTINETLLPLLKSMKTRELKILEAILKVYSTSSEILDEEHVVELIIPQLWLLSISSNLSTSLYQNYNDVINGISQRIQSNHMKKLKSLEGSKPKDDEDDGEKFRHLLYGKEDHTEQPAKLHIGLRDTPLSPTTNNTKSKPAAKPLSLKPKPHKTDLNFGSNSNGNIPKAVQNLRNTSSLSNNFDDDQFDDFVSAPSTRNTTPTPNTGIDWSSESGKIGGYGVLQPKSQSPSMKTPTMNTSMNTSMNTSMNTTSNLPPGFSTQLMQPMKRGNSVNKSNNGWNGGDSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVI9</accession>
    <name>A0A1E3NVI9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_65408</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036353.1">
        <property type="nucleotide sequence ID" value="XM_019183906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVI9"/>
    <dbReference type="STRING" id="683960.A0A1E3NVI9"/>
    <dbReference type="GeneID" id="30201152"/>
    <dbReference type="OrthoDB" id="3648309at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015123">
        <property type="term" value="F:acetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051633">
        <property type="entry name" value="AceTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047622">
        <property type="entry name" value="GPR1_FUN34_YAAH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038013">
        <property type="entry name" value="AceTr_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123:SF1">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01114">
        <property type="entry name" value="GPR1_FUN34_YAAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="277" mass="29942" checksum="A5D019476E2CEF33" modified="2017-01-18" version="1">MSDIEKQATPPIHRDSNTPTYAGNNPGFNDAEPEVSKISHGGENNEYIFIGNTKVLKSELVSAFGGTLQPGLAAPSIHKFANPAPLGLCGFALTTFMLSMYNARAMGITTPNVVVASAFWYGGLVQLLAGMWEIALENTFGGTALSSYGGFWLSYAAINIKWFGIGEAYEDKQELANAVAFFLLGWVIFTFGILLCTLKSTVAFFSLFFFLEITFLLLTIGEFSGKVGVTRAGGVFGVITAFIAWYNAFAGIANRENSYIIAKAVPMPGSMERLKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVL0</accession>
    <name>A0A1E3NVL0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">21S rRNA pseudouridine(2819) synthase</fullName>
            <ecNumber evidence="7">5.4.99.43</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Pseudouridine synthase 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Pseudouridylate synthase PUS5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Uracil hydrolyase PUS5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_36064</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Pseudouridylate synthase responsible for the pseudouridine-2819 formation in mitochondrial 21S rRNA. May modulate the efficiency or the fidelity of the mitochondrial translation machinery.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>uridine(2819) in 21S rRNA = pseudouridine(2819) in 21S rRNA</text>
            <dbReference type="Rhea" id="RHEA:42556"/>
            <dbReference type="Rhea" id="RHEA-COMP:10113"/>
            <dbReference type="Rhea" id="RHEA-COMP:10114"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.43"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.43" evidence="7"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036385.1">
        <property type="nucleotide sequence ID" value="XM_019182394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVL0"/>
    <dbReference type="STRING" id="683960.A0A1E3NVL0"/>
    <dbReference type="GeneID" id="30199640"/>
    <dbReference type="OrthoDB" id="428658at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160143">
        <property type="term" value="F:21S rRNA pseudouridine(2819) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004730">
        <property type="term" value="F:pseudouridylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluA-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF81">
        <property type="entry name" value="21S RRNA PSEUDOURIDINE(2819) SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="12">
        <location>
            <begin position="15"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ57178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="238" mass="27207" checksum="6AAF81147732C4B9" modified="2017-01-18" version="1">MSVKSILPVLFENSHYIVVNKPAGIHSQHSKASLKARAQDEVVPLLIDQYPDLKPVHRLDLRVTGGLLIARGRHDARMFSNNLRSGGDKGYKFKRRYIGITRAISEFKPIENVDDPYGPFSTTKFIRPESGIREDLQLIIMQLETGKKHQIRKHLSEVLSLPIVNDKKYGSSIIRGNDQQLGLHSSFIYTRVGNQLNKHFIPIQQGADDLWKGFVNEDGYFNEDINDILENFDERLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVN0</accession>
    <name>A0A1E3NVN0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_81386</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036372.1">
        <property type="nucleotide sequence ID" value="XM_019185225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVN0"/>
    <dbReference type="STRING" id="683960.A0A1E3NVN0"/>
    <dbReference type="GeneID" id="30202471"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990439">
        <property type="term" value="F:MAP kinase serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071852">
        <property type="term" value="P:fungal-type cell wall organization or biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000054">
        <property type="entry name" value="Protein phosphatase type 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF837">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="6">
        <location>
            <begin position="15"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="279" mass="31507" checksum="F795F567D98E9EC4" modified="2017-01-18" version="1">MSHSTSNKDYKLSYSVGVAEDVNKIYRPTMEDVHTYVENFVERLDWGYFAIFDGHAGKQAAKWCGQNLHSIVEKKLIEDENEDVRDILNESFLEADENIVKTVEGSSGCTAAVAILRWELPENKTASELTDLSQHKRMLYTANVGDTRIVLFRNNKAIRLTYDHKASDKHEINRIEKSGGLIMKSRVNGMLAVTRSLGDQFFKDLVIGSPYTTSVELNKDTDKFLIIACDGLWDVISDQQACELIRDIEDSTLAAKKLVEHALNKLTTDNITVMVISLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVT1</accession>
    <name>A0A1E3NVT1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_36177</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036526.1">
        <property type="nucleotide sequence ID" value="XM_019182404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVT1"/>
    <dbReference type="STRING" id="683960.A0A1E3NVT1"/>
    <dbReference type="GeneID" id="30199650"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000017">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004762">
        <property type="entry name" value="Amino_acid_permease_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00913">
        <property type="entry name" value="2A0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF17">
        <property type="entry name" value="GENERAL AMINO ACID PERMEASE AGP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="113"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="432"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="479"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="515"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="85"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="588" mass="64900" checksum="FA628E079545E8DF" modified="2017-01-18" version="1">MVDNIKKGGVDIYPEVSDKAESSSFQEAHFVNYNNSNESKWQKFKDSFKRLEDDIDPNLSEAEKAEIRRYRNEGEKLNKSLKTRHMILIAIATGIGTGLLVGSGSSLNKGGPAGLIIGTVIVGSMLVNVMEAAGEMALTYGDLTGGFNAYTSFLVDESFAFAVSWNYCIQWLCVMPLELVTASMTIKYWTTTINPDAFVAIFYVLIVVINFAGSAGYGEAEFFFNSAKSLMLVGFIILSIILSVGGAGDHHYYGAHYWHDPGAFANSFKGVCAVLINMAFALGCTEANAFMSSEAKNPRRAIPSATKQVVYKVVFFFIVPLFLIGLLVPYNSPELLGSSGSKTHASPFVIAVSKVKVVPHIVNAVILLAVLSVGNSALFIASRTLQSLAEQGFAPSWFNYIDRTGKPLRALAVSSIIGLFSFIAAYDKQETVFNWLLSISGLSQIFTWTGICLGHIRFRAAFKAQGYSTNELGYKSKTGVWGSIYGVVINFLILIAQFWIALWPIGGDGKANANSFFQNYLGAVVALVFYVAHKLWHRNWKLWIPADQIDLRKGRKIFDADVLAQEDMEETQRIKNSPFYVRAYHFWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVT2</accession>
    <name>A0A1E3NVT2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial Rho GTPase</fullName>
            <ecNumber evidence="15">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_36764</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 15">Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Mitochondrion outer membrane</location>
            <topology evidence="2 15">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the mitochondrial Rho GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="15"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036009.1">
        <property type="nucleotide sequence ID" value="XM_019182471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVT2"/>
    <dbReference type="STRING" id="683960.A0A1E3NVT2"/>
    <dbReference type="GeneID" id="30199717"/>
    <dbReference type="OrthoDB" id="10020961at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015886">
        <property type="term" value="P:heme transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055091">
        <property type="term" value="P:phospholipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010821">
        <property type="term" value="P:regulation of mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01892">
        <property type="entry name" value="Miro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000011">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000127">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000553">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000572">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013566">
        <property type="entry name" value="EF_hand_assoc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013567">
        <property type="entry name" value="EF_hand_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
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        <property type="entry name" value="Miro"/>
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        <property type="entry name" value="Miro-EF-hand_domain"/>
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    <dbReference type="InterPro" id="IPR020860">
        <property type="entry name" value="MIRO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819:SF1">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08355">
        <property type="entry name" value="EF_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Mt_Rho_GTPase"/>
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    </dbReference>
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        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51423">
        <property type="entry name" value="MIRO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="13 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 15" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Miro" evidence="17">
        <location>
            <begin position="3"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="186"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="306"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Miro" evidence="17">
        <location>
            <begin position="427"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ56802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="639" mass="71701" checksum="31D2A81CD4BAF1F3" modified="2017-01-18" version="1">MSKDTIRVVVCGDEGVGKSSLITALIKDTFVPNIQSVLPAITIPRDFSSSPYSPNSTVLIDTNSQDVPALQKEIRKADVIWLVYSDHYTYERISLYWMNIFRSMGVNLPVVLCANKSDSQTSLDSERTISDEFIPILKEFKEVESCIRCSAKEKYNVSQAFYLCQRAVTHPVSPLFDAKEGNLKPAATDALARIFFLCDKDQDGFLNEDEFLALQRKCFGRSLDINDLAEIRETVNQVKAGCFGERGLTEDGFIILNKIFAERGRHETTWGILRTFHYTDSLSLNDKFLYPKLDVPTNASVELSPIGYRFFVDLFLLFDKDNDGGLNDAELESLFRPTPGMPQEWVETNFPQSTVRNEQGYVTLQGWLAQWSMTTFLDYKTTLAYLAYLGFESSTRNGGTTTALKVTKPRKERVRSGKNYRVPVNDRTVFNCFVLGGSGSGKSSLLEAFLGRQYSEVYNPTIKARIVVNSVELKGGKQVYLVLEELGELEPAILDNQSKLNKCDVLCLTYDSSDPNSFHYLVELASKHPLISSVPTIIVALKADLDRQQQRFDIQPEEYTTSLNIPPPLHVSSTWPSSLTELFVQLVESSHVPSLATPGLEPEVKSDNLQNVVLATSAVGFMSIITLWIWKMTLQQKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVU4</accession>
    <name>A0A1E3NVU4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Maltose/galactoside acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_65550</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036498.1">
        <property type="nucleotide sequence ID" value="XM_019183932.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVU4"/>
    <dbReference type="STRING" id="683960.A0A1E3NVU4"/>
    <dbReference type="GeneID" id="30201178"/>
    <dbReference type="OrthoDB" id="25818at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051159">
        <property type="entry name" value="Hexapeptide_acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024688">
        <property type="entry name" value="Mac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416:SF54">
        <property type="entry name" value="ACETYLTRANSFERASE, CYSE_LACA_LPXA_NODL FAMILY (AFU_ORTHOLOGUE AFUA_2G08430)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416">
        <property type="entry name" value="SIALIC ACID SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12464">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01266">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Maltose/galactoside acetyltransferase" evidence="3">
        <location>
            <begin position="29"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="219" mass="24890" checksum="86495FF561C2662F" modified="2017-01-18" version="1">MTERFEKDFALIKYAYENFNMVPKGNEEYERMISGMQYNRFVPELKVPRIKTHEKCQRYSSMKLDDFHNPQEFEISQKHQLKQIFGKIDNTSIIEPPFFLDYGFNIEIGSYTTIAPFLTIIDGGIVKIGSNVKLGPRVTFITTTHPLDTVKRVDEEYEWARGINIGDFVRIGANVTILAGVSIGDNVVIGENSVVMRNVPSNAVFSGIPARMVKKDGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVY0</accession>
    <name>A0A1E3NVY0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mannose-P-dolichol utilization defect 1 protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_18539</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MPDU1 (TC 2.A.43.3) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035944.1">
        <property type="nucleotide sequence ID" value="XM_019180793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVY0"/>
    <dbReference type="STRING" id="683960.A0A1E3NVY0"/>
    <dbReference type="GeneID" id="30198039"/>
    <dbReference type="OrthoDB" id="271506at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016817">
        <property type="entry name" value="MannP-dilichol_defect-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12226">
        <property type="entry name" value="MANNOSE-P-DOLICHOL UTILIZATION DEFECT 1 LEC35 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12226:SF2">
        <property type="entry name" value="MANNOSE-P-DOLICHOL UTILIZATION DEFECT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023381">
        <property type="entry name" value="MannP-dilichol_defect-1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ56737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="27830" checksum="D3294284ABCC03D8" modified="2017-01-18" version="1" fragment="single">LRPFTGALPPQVINYGRQLYTRPVFDTIILKLNLFSPESAPLLKEFISKSLGYGIVGSSGIVKLPQILSILSKGSVQGLSFFSILLETISNLITLSYNFRQNNDFITFGESAFLSVQNLLILLLILYFSGLSKYINGFVGLASLAFYSLFANPDGNPGVLSNVDVKNLTKLTIPLIILSKLPQISNNFRNKSTGQLSIVSVFAGLLGAIARVFTTLSSGIQDNLILFGFGASLLLNLVLFLQIVVYKNKPISKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW16</accession>
    <name>A0A1E3NW16_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_65564</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 14 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036512.1">
        <property type="nucleotide sequence ID" value="XM_019183936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW16"/>
    <dbReference type="STRING" id="683960.A0A1E3NW16"/>
    <dbReference type="GeneID" id="30201182"/>
    <dbReference type="OrthoDB" id="205099at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055122">
        <property type="entry name" value="Med14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013947">
        <property type="entry name" value="Mediator_Med14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12809">
        <property type="entry name" value="MEDIATOR COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12809:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08638">
        <property type="entry name" value="Med14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit MED14 N-terminal" evidence="10">
        <location>
            <begin position="21"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ57305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="820" mass="96114" checksum="8049D655C3B0DEAF" modified="2017-01-18" version="1">MESEIRSQQPPEIPHITTNIIPLSDILRKFSEFSYAELSHIIQIQPTNEAKKKKLLELIVYLRQEYVRLYVLTKWSKISAQDFSKLIDLLAWLREQTNYFTNLIWATKSINQSLLSAKLPNPDIITALEVFTEGRPTLPSHNLIESKITPQKILQTLKDLNVILSMKFALVENIPDKFLNYEIKDGRIFIYTDDYEFQVSVIDDNSPFFMIDFKFNFGDFEPSRKLLRISNDALRIGGFNELNKILTNYTNTMKLYMIHLKLNGLKNIKHIYHADKFQIVIHYWINSYVFKNSYIEIGLNKDNNIIYRWFKQAQFVETFQDIGFIDDFLNVIYYKHAVSILDQIDDITVNKSVLQINEKTGLFYFKNSTPLMNSFLKKLNTDDFKNINMNLKLLRMDHKYMEISTILSVTGWIQNDIIKLAPHEVNKLGQEKLLNYQQKENTVFLSRNLKFYTRKEWPSNWFLVLLIDTKIKSFIGNVRSIAGQWTISSLNELNIETFDYRTSKGLIDYVSKKIILHLITNELGSAVYKIIKDDTILIQTDSFISIPDTSNTLYLNFKLEPNKKNMMLKLKGKLNNSLSLEDFVIDKNGVFEVFETVEFKRFSILANIIAKLQKLSKVIGLINFLKLESLNLLNVKLDEIVFKYGSQICTLRDGFDIELPTTNPHNICLVSIKRYLHKRGIGKLFKYLQESNVLVEKLNELATQESTDKNDYDPSKLRYEVIPKNLNLFTVMYYNNYKPERQCLDLNIEMKIKNGKYCYLITFDRDSEFKSEFTMKGELYNSMKHKFKLVPLRNGIMCDESGIDQVLGYFHDKIMTKLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW57</accession>
    <name>A0A1E3NW57_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_35487</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ELP5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036561.1">
        <property type="nucleotide sequence ID" value="XM_019182311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW57"/>
    <dbReference type="STRING" id="683960.A0A1E3NW57"/>
    <dbReference type="GeneID" id="30199557"/>
    <dbReference type="OrthoDB" id="166907at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19496">
        <property type="entry name" value="Elp5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019519">
        <property type="entry name" value="Elp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15641">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15641:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10483">
        <property type="entry name" value="Elong_Iki1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="293" mass="33586" checksum="ED3A048DD96306F2" modified="2017-01-18" version="1">MSGVTQNSTVLLNRLFTLKESSPLLLILDTIAQSSFYLVQEFVHNAPDNTNIIFVSFETIHKPKFANVFIEAYDIPLKKLVETLNTHYDPSKKNLVIIDSINYIATEYLSQFITTIMKPNLTVITTLHTSLPEADVNPQFNNYPTSHKLLSYIASSIFEIIPTFEDDEELENQLNKLNIVKNMNNAVFQINLINRRKSGRSLSYKFEINTLNHEYNFVLPTDPNENNQPMDSQSLLKGLTTFNLTTSDKQKLAKDQVELPYLDAQSFNSGGAIVYEFEKDDDYDEEDPYEDPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW84</accession>
    <name>A0A1E3NW84_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_58458</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036149.1">
        <property type="nucleotide sequence ID" value="XM_019182820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW84"/>
    <dbReference type="STRING" id="683960.A0A1E3NW84"/>
    <dbReference type="GeneID" id="30200066"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000065">
        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF36">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G08340)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="462"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="76"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ56942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="508" mass="57148" checksum="C065CA5BE477BF5C" modified="2017-01-18" version="1">MSREVKTEVSHNLESIHDSLDLEDAIPTTIPFKKSLKLFFSTKSPEKEPRSKFFFWSPPGQTKFEKKLVFKLDTAILSYVCLSYFIRYLDATNIASAYVTGMKEDLSITGEQYVWLGQLFSSAYCFSGFFATIVLTRVRFSRMLPVLEIFWGIMTLAIYKSENFKTVAVLRFFQGLAEGAAWPSIHYILGSWYTKRELGKRVSIFTASGIAGVLLSGLIQSGLQRNLNGRHGLAGWRWLFIVDACLTFPVAFLGFFIFPDSPENAKVKFYLSEEEIQFAKNRIKVNGKDRVDNLDFSTFKRVFTGYQWYLFVLCWIFWGFAGQLSNYMGIVLKALGYNVYDRNNIPSGISGVGIVLAIATGFAVDIIGARIHVALSLTTIWIVGLAIIKAWDVPRGALIFGYMCLGAYTAVSPVIVGWCNELCKEDNQLRAATIGSLNLFSGLLGIPFSIELLNTDYAPKYTRAVTASLILAVMLFFYFFLVLAFDRYQGRKRDYIKSTTSSDAKSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWC4</accession>
    <name>A0A1E3NWC4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_85660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036716.1">
        <property type="nucleotide sequence ID" value="XM_019185924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWC4"/>
    <dbReference type="GeneID" id="30203170"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="282" mass="33130" checksum="4E693914A51CC1AB" modified="2017-01-18" version="1">MHLHNVHLRDSPIVLLKEALKSLYRKDKKEAVKSETPELLPKFFSGLRVWKHKAKKGECYILSTKTLNLAKTFKIISQGELQPSSPLKVKFFQEKLHWYSSNPVFFLSNTRYEYFDKDMMHYSFDSLRKDDSEELLGDQYELTSQKYISLHGMLTFAASESDEKLCEKIRYAIKKTLFDKACFKTTDSYNFCLMKVPCSCKLSSYRSYWPQKCDTNHVVGFIYRSGEGEKIFHKCISDRIENYGDTFRRFKTKFKVFNEGPFGCSDEYPAYCQTREEAKACK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX10</accession>
    <name>A0A1E3NX10_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_97120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036918.1">
        <property type="nucleotide sequence ID" value="XM_019186563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX10"/>
    <dbReference type="GeneID" id="30203809"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="58" mass="6719" checksum="57239FAA3814A68D" modified="2017-01-18" version="1" fragment="single">MSNEVSRSNCLRILRFNLVELNDELKTNRFSHRLSFLNNDNFFEIFSTTGSSTDTSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX54</accession>
    <name>A0A1E3NX54_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_81087</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036968.1">
        <property type="nucleotide sequence ID" value="XM_019185174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX54"/>
    <dbReference type="STRING" id="683960.A0A1E3NX54"/>
    <dbReference type="GeneID" id="30202420"/>
    <dbReference type="OrthoDB" id="282973at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00842">
        <property type="entry name" value="MPP_ASMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041805">
        <property type="entry name" value="ASMase/PPN1_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340:SF27">
        <property type="entry name" value="ACL091CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133574" description="Calcineurin-like phosphoesterase domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="4">
        <location>
            <begin position="286"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="665" mass="75661" checksum="6417401044A3BCF4" modified="2017-01-18" version="1">MKVSSMLLGTLAALSTVVNAVPLFNLELTLDTTVFTPSDAKLIVDFARQLTLIDQFNGTNTTKCDKCIKRLQLGKTITMTKPNLVYPVFTKWCIDNKVANKVTCETNYGRNTVDNSTFGTNFANMLYLMDPSSYDGQLYCHFQDTKSCPLPKTPKVDLSGYWPPKQPKHHVAPTPGNETYNVLHVSDFHIELNYQIGAEANCSRGMCCTPYSKNAAKLPQGYSFTANLTEDQIGNLSYTPAWYDEDNELQYGEPVDVFKNGSDVWYPANTFGHYECDAPPVLVNSSLNSVAKFQEELDLDFKFSIFTGDLVEHDEAQYKSHEFVIESEEIVFRDMKKTLKGIPVFSVLGNHDTFPYAQLAQEKSGFGNLFDWNADLMADMWEDYGWINSTTAQHVRKHYSGFAVTTSTDLKIIVLNSNAYYVKNLYMYWNATDIDSFGQFQFLIDELVDAEAKDQRVWIMAHIPFIKEALPLPAEVFTQIIKRFSPYTIAGIFFGHTHLDQFNLLYDGGVGNKTEQNLVANAWISQALTPLTENNPGWRYYSIDTKTHSVMNSFNYYTKLNDTFTNGNEEPIWEFEYSAREAYESVAEWPDNAPLNATFWHRVATGVNNTEEIAQTYSNYKKRFSPFTPNCIGTDECDGSWCFLSSFTAKDHQECMKMYGQPDIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXE3</accession>
    <name>A0A1E3NXE3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_57556</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036549.1">
        <property type="nucleotide sequence ID" value="XM_019182807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXE3"/>
    <dbReference type="STRING" id="683960.A0A1E3NXE3"/>
    <dbReference type="GeneID" id="30200053"/>
    <dbReference type="OrthoDB" id="2130629at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17476">
        <property type="entry name" value="MFS_Amf1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF1">
        <property type="entry name" value="LOW AFFINITY AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="303"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="468"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="34"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="496" mass="54062" checksum="ECDC4F7380E7C78A" modified="2017-01-18" version="1">MSESFTLDTEKVDPPKVDAPSPSPSFNEISTPRLIIFVMLMAISQIFTQAALAQTVVPLKFVSKSFDVESSGELSWFTASYSLTVGTFILIAGQLGDLFGYKKLYLIGYTIFGLFSLICGFATFSHSAIFFDVMRALQGLGLAIAVPNSLGLIGGYFPHGSHSKNIALSLFGGVAPMGFLIGAVFSSLIAQFSWWPWYFWITGIASCIVLILGYIFIPKGIRGQDDKGSWKDFDYFGSLFGVTGLVLFNFAWNQGPVVGWETPYVYILLIIGILAIVGFFYVELKVSKRPLVPREVLTGDTGFVLACIISGWSCFGIWIYYQFQFSEFILHQSPLLSTAQMVPTMFAGLIAGISTTYMLKYLSTSVVMTISLCCFLIGSCLMGTRPVTQMYWAQKFPSAILTPFGMDISFPAATIVLSHHLKKEHQGVAASVVATCVNYSISIGLGIAGTVQRYVDPQDLKTLEGMRAAFYTGIGLAGLGVVVGLAFIIKQRALTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXW6</accession>
    <name>A0A1E3NXW6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Serine/threonine-protein kinase BUR1</fullName>
            <ecNumber evidence="4">2.7.11.22</ecNumber>
            <ecNumber evidence="3">2.7.11.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_18821</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="4"/>
    <dbReference type="EC" id="2.7.11.23" evidence="3"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037256.1">
        <property type="nucleotide sequence ID" value="XM_019180807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXW6"/>
    <dbReference type="STRING" id="683960.A0A1E3NXW6"/>
    <dbReference type="GeneID" id="30198053"/>
    <dbReference type="OrthoDB" id="28397at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009891">
        <property type="term" value="P:positive regulation of biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF233">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="7"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ58049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="335" mass="38015" checksum="5D0E3F459E1B2DFE" modified="2017-01-18" version="1" fragment="single">VCRLDRYQIIKQLGKGTFGIVYKGIHKQTGYYVAIKKILIHKGADCFPVTAFREIDILKNLQSPECKHIIELIDMIRDRPPPNDEYTNYDSSKAPKSIKKSFYMVFPYMSYDLSGILGNPSVTLTEPDIKSIMKQLLDGINFIHASGYLHRDIKAANLLINWEGVLKIGDFGLARSYGGPRPTLDKAGGGNYPLTEVVMTRWYRAPEVLFGDKLYTTAVDIWGIGCVFGELYERKPILPGKSDLDQAYDIFKLVGEANENNVTHMDRYKFEEKNIILKPTKRTLEERFAKSISSEGVDLLAKMLALDPERRITGLKALEHPYFNIQPLPTETVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXZ5</accession>
    <name>A0A1E3NXZ5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Lon protease homolog 2, peroxisomal</fullName>
            <ecNumber evidence="9">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_33613</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">ATP-dependent serine protease that mediates the selective degradation of misfolded and unassembled polypeptides in the peroxisomal matrix. Necessary for type 2 peroxisome targeting signal (PTS2)-containing protein processing and facilitates peroxisome matrix protein import.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10 11">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="9"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037286.1">
        <property type="nucleotide sequence ID" value="XM_019182092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXZ5"/>
    <dbReference type="STRING" id="683960.A0A1E3NXZ5"/>
    <dbReference type="GeneID" id="30199338"/>
    <dbReference type="OrthoDB" id="2411602at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000021">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03121">
        <property type="entry name" value="lonp2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027501">
        <property type="entry name" value="Lonp2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008268">
        <property type="entry name" value="Peptidase_S16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="ATP DEPENDENT LON PROTEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01046">
        <property type="entry name" value="LON_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="2 9 10" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 10" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="14">
        <location>
            <begin position="22"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="13">
        <location>
            <begin position="770"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="362"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Microbody targeting signal" evidence="9">
        <location>
            <begin position="999"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="363"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="active site" evidence="9 10">
        <location>
            <position position="871"/>
        </location>
    </feature>
    <feature type="active site" evidence="9 10">
        <location>
            <position position="914"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="521"/>
            <end position="528"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ58079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1001" mass="110732" checksum="FE4998423328D0FD" modified="2017-01-18" version="1">MGLPQNNGAAGAPASPSTEITLPIYKLSKSPIVLLPGILYRVTFSNQDASNFLNFYKNPKLHNLLIANLPKQGLLGVDEKNAEKVSKDSIQGSRALRKFLNGNQGNEGAYDWLIVGLLSQLSNDKVATISRIVGIVNDSKSIVITFQALTRGEVLLDDIEDTEPGFPFEAKIKAISNHVDLGTDYLHEKVPVFNKVTDELFDKIENFMKEYSLVCTFEKSIPQGKKYALTLSPLASALYQQFSNDFKNSWQKLINLIEKFNGNLDKLKSIKNSETFLKLLDLTIAVLPTAKLEFLGTLTVETRFDKFVEILQNFIELFNVLDKSVEYVENYYSKASDLEKSKIISNQLKSIKFSLDSIKPEKSSNYSPGTNTPTNRSNPFSGASSSGNSEEAEELQIVDKFINNIPSDVHPDGRQSLTKDYKRLKRMPTQHSEYQVLRTYFDVILDIPFGQYINSKEIEISKAQKQLDDDHYGLHHVKKRLLQYLSVLKLHDKNNLDESPNSPNELVKKPSSSSPILLFVGPPGVGKTSLARSIATALGRKFQRVSLGGVRDESEIRGHRRTYVGSLPGVIISALRKAGSMNPVILLDEIDKVSGGTGSNGKISGDPAAALLEVLDPEQNCNFTDHYIGFPVDLSQVLFLCTANELATISPPLRDRVEIVEISGYTAEEKVNIGSKYLLPKQIKLNGLPEGEVLLNESIWKKIVLEYIREPGVRNLERTIAAICRGKAVEFVENEESNYSPNIDENSLVKYIGLPIHPISKDLILRPKLSNHYGVVNGLSYNSDGSGGVLVFEVISIPLHSENSKSPTLIITGRLGETLTESIKIGLSFIKSTISRNLIHNLNSTEVLKQFNNSELHLHVPMGGIPKDGPSAGITITLALLSIALSKPVDPTIAMTGEITLRGKVLPIGGVGEKLLGASLFGIKTVLIPKLNRKDVIEHFYNDDDTKLVEFLNKGTDEPEVKVKEKLGIEIIYVDTFWDVIKAVWGNEVELGVDQILESHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY30</accession>
    <name>A0A1E3NY30_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_64154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037212.1">
        <property type="nucleotide sequence ID" value="XM_019183676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY30"/>
    <dbReference type="GeneID" id="30200922"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="98" mass="10449" checksum="EAD922BD533140AD" modified="2017-01-18" version="1" fragment="single">MAVTRNSNRRNTSSTSSTSSTTRVTKTRKPARKTKANPRVTAIENNISASHAASTSENTPNSTTSEENFSTNGAQQAALSAQNENFSNSTETDHETNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY54</accession>
    <name>A0A1E3NY54_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_101452</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037346.1">
        <property type="nucleotide sequence ID" value="XM_019180322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY54"/>
    <dbReference type="STRING" id="683960.A0A1E3NY54"/>
    <dbReference type="GeneID" id="30197568"/>
    <dbReference type="OrthoDB" id="8883818at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
        <property type="term" value="C:t-UTP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046351">
        <property type="entry name" value="UTP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44163">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44163:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="154"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="206"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="387"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="771" mass="87670" checksum="1D2FA304961DEAC7" modified="2017-01-18" version="1">MTVDVHRCRFVDYQPHTITGLSFSHKSTKKFSPADLRLAVARNNGDIEIWNPKNKWIHETTLYGGKDRSIEGLVWSTRESEQPRLFSIGGSTVITEWDLKTGLPLKHFDCNAAIIWSISINESQDKIAVGCDDGTVVVVDISGGPGSIEHFAFLQRQGSRVLSVTWKEDNYIIGGCADGRIRVWNFGGDSQLHKYSLLSTMKVDKSKHESTLIWSVIYLPNQNQIVSGDSSGSVKFWDFSHFTLLQSFKVHAADILTLTTDLSNENVFTAGVDRKIFKFQLIETSTNTNKKQLRWSNVSNRLFHSNDIRSMTSYESKNFNFLLSGGVEKSIVISSLKSFTDGQYRKLSIIPQEQHVTMNKSQNLVVMWQEQTVKIWRLLKRKLIDESDDEEDERMEDADAQEEDEANYKETFELAAKLTLADDENITSVALSQDGTILAVGRLTTTKLFKLSPKSNSSKLKVSKIDEQQIQAQGSKHLLFNGSDSLILTTPDNEIYRFNLDEESTDEQVIEYELSDLPRSKSKLPYIENINHIKLTHNNEKLVVSRVSGAIDLIDLNNSDKIESTPFLRLSSYINRIEVSLKNTLIVITHENKIYEFDLNNESNSAHLTNWSKINSEFLPKQFTSLQESPLGIFLDVSNRDKIWIYGSTCLNIPINRSKKDKKRNRHGVQIGVNEDIAVEDEGLDESDDEQQIENELLQDQVTRLLERNDFIKEVATGDEDKNGVPFAITRKYKPILYANSLLNEGEIIVIERPQFALSQPPAFKLNQIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYF7</accession>
    <name>A0A1E3NYF7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Deacetylase sirtuin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_22059</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037342.1">
        <property type="nucleotide sequence ID" value="XM_019180927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYF7"/>
    <dbReference type="STRING" id="683960.A0A1E3NYF7"/>
    <dbReference type="GeneID" id="30198173"/>
    <dbReference type="OrthoDB" id="420264at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099115">
        <property type="term" value="C:chromosome, subtelomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031934">
        <property type="term" value="C:mating-type region heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005721">
        <property type="term" value="C:pericentric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032041">
        <property type="term" value="F:histone H3K14 deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141222">
        <property type="term" value="F:histone H3K4 deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046969">
        <property type="term" value="F:histone H3K9 deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046970">
        <property type="term" value="F:histone H4K16 deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031508">
        <property type="term" value="P:pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902794">
        <property type="term" value="P:siRNA-independent facultative heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007654">
        <property type="entry name" value="NAD-dep_histone_deAcase_SIR2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF9">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACETYLASE SIRTUIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04574">
        <property type="entry name" value="DUF592"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="15">
        <location>
            <begin position="126"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ58135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="406" mass="46135" checksum="750AB21E0B8200E5" modified="2017-01-18" version="1" fragment="single">NTNAVPSTTTTTTQSSSASSSTTPPKGSKAPKRSRIKKVDGKWILPQVTREQCQEARKLLKTQGMDKFLDYYLPDNPRGSDMLYVVVILGFYSIKFDDDIDDEQNFMKIVKLLQRAMNRVLYMRTRLDDIYTVEHALQQIEKASKILVLTGAGISTSLGIPDFRSSKGIYSQVKHLGLSDPQEVFDLETFREDPTIFYSVAHMILPPLGSYTPLHAFIKLLEDKGKLLRNYTQNIDNLEANVGISKEKTIQCHGSFAAATCQTCRWKIDGSKIFKYIKELELPVCPNCYKEREKRFNRDENYYPRSYGVMKPDITFFGEDLPKKFHDSIKLDVQECDLLICIGTSLKVAPVSEIVNMVPAEVPQILINKDLVEHAEFDVSLLGYCDNVAAYLSNKLGWKLNHPDYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYJ7</accession>
    <name>A0A1E3NYJ7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">tRNA:m(4)X modification enzyme TRM13</fullName>
            <ecNumber evidence="3 15">2.1.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_21551</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 15">tRNA methylase which 2'-O-methylates cytidine(4) in tRNA(Pro) and tRNA(Gly)(GCC), and adenosine(4) in tRNA(His).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>adenosine(4) in tRNA(His) + S-adenosyl-L-methionine = 2'-O-methyladenosine(4) in tRNA(His) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43196"/>
            <dbReference type="Rhea" id="RHEA-COMP:10401"/>
            <dbReference type="Rhea" id="RHEA-COMP:10402"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74477"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>cytidine(4) in tRNA(Gly)(GCC) + S-adenosyl-L-methionine = 2'-O-methylcytidine(4) in tRNA(Gly)(GCC) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43192"/>
            <dbReference type="Rhea" id="RHEA-COMP:10399"/>
            <dbReference type="Rhea" id="RHEA-COMP:10400"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 15">
            <text>cytidine(4) in tRNA(Pro) + S-adenosyl-L-methionine = 2'-O-methylcytidine(4) in tRNA(Pro) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32767"/>
            <dbReference type="Rhea" id="RHEA-COMP:10397"/>
            <dbReference type="Rhea" id="RHEA-COMP:10398"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the methyltransferase TRM13 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.225" evidence="3 15"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037395.1">
        <property type="nucleotide sequence ID" value="XM_019180906.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYJ7"/>
    <dbReference type="STRING" id="683960.A0A1E3NYJ7"/>
    <dbReference type="GeneID" id="30198152"/>
    <dbReference type="OrthoDB" id="258806at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106050">
        <property type="term" value="F:tRNA 2'-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007871">
        <property type="entry name" value="Methyltransferase_TRM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039044">
        <property type="entry name" value="Trm13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022776">
        <property type="entry name" value="TRM13/UPF0224_CHHC_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021721">
        <property type="entry name" value="Znf_CCCH-type_TRM13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12998">
        <property type="entry name" value="TRNA:M(4)X MODIFICATION ENZYME TRM13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12998:SF0">
        <property type="entry name" value="TRNA:M(4)X MODIFICATION ENZYME TRM13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05206">
        <property type="entry name" value="TRM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11722">
        <property type="entry name" value="zf-TRM13_CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05253">
        <property type="entry name" value="zf-U11-48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51800">
        <property type="entry name" value="ZF_CHHC_U11_48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 15" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="11 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHHC U11-48K-type" evidence="16">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ58188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="377" mass="43912" checksum="122F51BC500ED789" modified="2017-01-18" version="1" fragment="single">KKNRRCPLTRKKDVQFCAEHLKQKQDDVRIPCPVDPRHTIWEKDLKNHIEKCKAKATDATKVSWFKLNHNCEVPKEKAEEIVIDYKKWIPIVEKIYTNFDELKLIKLEHDGVKDRMEELGNQKHAIQQSSLISHLDNKELLSNELNYIEFGCGRAELSRYIAKSIMFKTSKTADFILVDRSPTRLKLDSKMVKDAEEHDLKTKIYRVKIDIKDLFIDDIVTKEFNNNKNFVGVSKHLCGAATDLTIQCILQNPILNSRFQGMIVAMCCRHCCDYDMLSLYSKQYLKKFGIEREGFKHLMKFASWAVNGRRPGMKDEDGSDHPSGLSIKEREDLGLKARRIIDESRKFALEQNGYKVELCHYVPREISLENTCMIVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYP2</accession>
    <name>A0A1E3NYP2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">precorrin-2 dehydrogenase</fullName>
            <ecNumber evidence="2">1.3.1.76</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_34734</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.76" evidence="2"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037450.1">
        <property type="nucleotide sequence ID" value="XM_019182221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYP2"/>
    <dbReference type="STRING" id="683960.A0A1E3NYP2"/>
    <dbReference type="GeneID" id="30199467"/>
    <dbReference type="OrthoDB" id="1721126at2759"/>
    <dbReference type="UniPathway" id="UPA00262">
        <property type="reaction ID" value="UER00222"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043115">
        <property type="term" value="F:precorrin-2 dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.110">
        <property type="entry name" value="Siroheme synthase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3280.10">
        <property type="entry name" value="Siroheme synthase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028161">
        <property type="entry name" value="Met8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028162">
        <property type="entry name" value="Met8_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028281">
        <property type="entry name" value="Sirohaem_synthase_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330:SF1">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13241">
        <property type="entry name" value="NAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14823">
        <property type="entry name" value="Sirohm_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14824">
        <property type="entry name" value="Sirohm_synth_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75615">
        <property type="entry name" value="Siroheme synthase middle domains-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Siroheme synthase central" evidence="7">
        <location>
            <begin position="159"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme biosynthesis protein Met8 C-terminal" evidence="6">
        <location>
            <begin position="190"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="269" mass="30956" checksum="ED25BDDF03E269EF" modified="2017-01-18" version="1">MSESEFPEIQPNGSLILAWQVKNRHVLVIGGGEVAASRIYHLLNANAKITVIAPTMNSEIEYRESKGLLHAVHRRQFQDSDLKLYEQTAPQLEKFDEEEYEKIDKYLRESRFELVLVAIDDPIESKKIYYKCKLQGLNVNIADVPPLCDFYFGAIFRKGDLQVMVSTNGKGPRMARLIKDKIADSFSDYEIDKTIKNIGDLRKLLRSKCSGSDNDSIKVRMEWMTKVTDSYTFKQWSDIDIRKVIEYFPGMPPSYDELKNNSLLATLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYY2</accession>
    <name>A0A1E3NYY2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RanBD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_85304</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037427.1">
        <property type="nucleotide sequence ID" value="XM_019185873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYY2"/>
    <dbReference type="STRING" id="683960.A0A1E3NYY2"/>
    <dbReference type="GeneID" id="30203119"/>
    <dbReference type="OrthoDB" id="2357150at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13179">
        <property type="entry name" value="RanBD_RanBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000312">
        <property type="entry name" value="Ran binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045256">
        <property type="entry name" value="RanBP1_RanBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF87">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE RANBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RanBD1" evidence="2">
        <location>
            <begin position="76"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="212" mass="24040" checksum="A080B1CD5514462E" modified="2017-01-18" version="1">MSDAKEETKPVATATEEAKPTAATTQNPPTANVFSMFGGASKKKEVKEDDEEKKDEKKDGEAAAEGDDAPESPDVHFEPLVHLEKVDVKTNEENEEVLFKVRAKLFRFHPDSKEWKERGVGDVKFLQHKETKKVRILMRRDKTLKICANHFISPEYQLKPNVGSDRSWVYNVTADVSEGEPEAQTLAIRFGNKENADKFKETFEKAQESNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ32</accession>
    <name>A0A1E3NZ32_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S20</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_64567</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037636.1">
        <property type="nucleotide sequence ID" value="XM_019183763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ32"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ32"/>
    <dbReference type="GeneID" id="30201009"/>
    <dbReference type="OrthoDB" id="10248551at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.600:FF:000004">
        <property type="entry name" value="30S ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_uS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018268">
        <property type="entry name" value="Ribosomal_uS10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005729">
        <property type="entry name" value="Ribosomal_uS10_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01046">
        <property type="entry name" value="uS10_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00361">
        <property type="entry name" value="RIBOSOMAL_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS10" evidence="6">
        <location>
            <begin position="20"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="117" mass="13128" checksum="8701293FC97B0ECA" modified="2017-01-18" version="1">MADVQKDKVTENQPQIHKIRITLVSSKVKQLEKVSANIIRNAETYDLIKKGPVRLPTKVLKISTRKTPNGEGSKTWDTYEFRIHKRVIDLQAPAAIVKKITQITIEPGVDVEVTVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ50</accession>
    <name>A0A1E3NZ50_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_96530</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037718.1">
        <property type="nucleotide sequence ID" value="XM_019186517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ50"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ50"/>
    <dbReference type="GeneID" id="30203763"/>
    <dbReference type="OrthoDB" id="270318at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="226" mass="26053" checksum="938B6C937253EE9F" modified="2017-01-18" version="1">MVFETNSILVQRVSIKSYQQGSTSYPNELCSYGEGLYSQLNYIVQNLLLSNQLSPSTIKLVEESSELQNLITEISAHIGQSTGIASIVIGTKYYAQNKNQINLPIDIMTKHDLSQESLLRLFQGHEDQKAEIEAKLKDVIYETCVVANDHLLTAREKLKAVKEEISKIISTTEDELILRKSKTWKKQVPDCLFLEKNDFDILSKNIENNYWKLVWNSYRNYQKRQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ77</accession>
    <name>A0A1E3NZ77_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_33504</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037723.1">
        <property type="nucleotide sequence ID" value="XM_019182079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ77"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ77"/>
    <dbReference type="GeneID" id="30199325"/>
    <dbReference type="OrthoDB" id="413460at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061635">
        <property type="term" value="P:regulation of protein complex stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18000">
        <property type="entry name" value="DEXHc_ERCC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000094">
        <property type="entry name" value="DNA excision repair protein ERCC-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058951">
        <property type="entry name" value="WHD_Rad26_CSB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25875">
        <property type="entry name" value="WHD_Rad26_CSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="321"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="651"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="946"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="53"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="270"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="953"/>
            <end position="968"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ58516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1057" mass="120964" checksum="47CD30F02B639E27" modified="2017-01-18" version="1">MSTSKEEAGGSDSSQVVDQPSENNELQNLGIKLVEQTSLEHKIQANANRLITQKEIESDLKRLEKSKQQLDRVKYELRKLNDRLNNPRTKISQKDEIRDQIKSMTELQLTPTENDIKDIEERLKKNENASEINEITEAGARLPNETEKDFLIRTGKITAFGNSAGFMVEEDKDHTEPGEVVSHQNLRIPGFSQVNQPIEVEDEDDVNEFGVKRKHETDDEFEDENEEEFKKPKLLTDDDIRNIDDGVESFYQDRLNTWVYRRSQLRSHKNHETNQDDNNKPEWLKPHPNLPDAVLNADFKVPGDIYPSLFDYQKTGVQWLYELYSQKHGGIISDEMGLGKTIQIISFLAGLHYSGKLDKPVLIVCPATVMTQWVNEFHTWWPPLRTMILHSIGTGMKGSFKEEDYEKLLLKEADEDGDEPSLRSLKANSNVNTIIDTLLTKGHVIITSYVGLRIYEEQLLNIDWGYVVLDEGHKIKNPNSNITILSKRLKTYNRVILSGTPIQNNLIELWSLFDFIFPGRLGTLPIFQEEFETPIKLGGYANANNLDVKVGYQKAVILKELIQPFLLRRVKMDVARDLPSKQEYVLMCRLTQYQKEKYQEFLKSTEFKVNSYLGAIDTLRKICNHPDLADVHFIEGQKGYGDPAKSGKLQVVKSLLTQWRQQKHKVLLFTQTKQMMVILEKFLNITFGDDYKYMKMSGETGIGKRQDMIYEFNNEDYDLFLLTTKVGGLGVNLTGADRVIIFDPDWNPSTDLQARERAWRLGQKKEVLIYRLIIGGAIEEKIYHRQIFKQLLTDKILKDPNQKRFFKNSELHDLFTLTDFDDNSESGNVFKTRNQTKKNRDDLANIQEVQGVTKLEKFKDDSQKETEDERLLSGLFKNTGAVTQAIKHDDVVKTHSKPKPDLFDREAKKAANDALEALKRSRRQVKKAGLGVPTFTGKFGLAGRSASPALGNRSRSGTPLNPKQSSTSILSNIRKNQEATSLNGNSSELSKNSKIIDEMRSYLVKQEGCYAKSGDILDGVSVQINDKKDLTMVRSMLKGIATWDTDRSGWVLKDEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZF8</accession>
    <name>A0A1E3NZF8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Multifunctional methyltransferase subunit trm112</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eRF1 methyltransferase subunit trm112</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_85472</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRM112 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037645.1">
        <property type="nucleotide sequence ID" value="XM_019185896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZF8"/>
    <dbReference type="STRING" id="683960.A0A1E3NZF8"/>
    <dbReference type="GeneID" id="30203142"/>
    <dbReference type="OrthoDB" id="2187549at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035657">
        <property type="term" value="C:eRF1 methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043528">
        <property type="term" value="C:tRNA (m2G10) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160102">
        <property type="term" value="F:tRNA (guanine(10)-N2)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070476">
        <property type="term" value="P:rRNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000021">
        <property type="entry name" value="Multifunctional methyltransferase subunit trm112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039127">
        <property type="entry name" value="Trm112"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005651">
        <property type="entry name" value="Trm112-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773:SF0">
        <property type="entry name" value="MULTIFUNCTIONAL METHYLTRANSFERASE SUBUNIT TRM112-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773">
        <property type="entry name" value="UPF0315 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03966">
        <property type="entry name" value="Trm112p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158997">
        <property type="entry name" value="Trm112p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00069342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00083044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="129" mass="14749" checksum="57ABD2CEB871C173" modified="2017-01-18" version="1">MKFMTTNFVKCSIKTCDSTTDSFPLKYQNCSLVQEEQEFNPEFIVSILDRLEWDATLKVAQDLGNTSLPPVKPENIDLESEESLPLLKDLHSLLLETNITEGEMICNNCQHIYYIKNSIPNFLLPPHLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZH4</accession>
    <name>A0A1E3NZH4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Asparagine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="2">6.1.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Asparaginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_57438</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="2"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037712.1">
        <property type="nucleotide sequence ID" value="XM_019182803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZH4"/>
    <dbReference type="STRING" id="683960.A0A1E3NZH4"/>
    <dbReference type="GeneID" id="30200049"/>
    <dbReference type="OrthoDB" id="43906at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070145">
        <property type="term" value="P:mitochondrial asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04318">
        <property type="entry name" value="EcAsnRS_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000016">
        <property type="entry name" value="Asparagine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003037">
        <property type="entry name" value="PRK03932.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF34">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="149"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="476" mass="54059" checksum="A7E798D09B5CED5E" modified="2017-01-18" version="1">MRSLFRTNVTLKRFQSTLPPSISQLFQNPPPLESELTINGYIKSVRSLKKVSFVDLSDGSSHQNLMCVLSPAQATGLKTGQSIELKGFWVKSKGKEQPFEFKVLPGEESLKIVGNVKDDFQLQKKAHSIQYLRSLSTLRWRTSYLSSILRFRSFVDLKLHDFFQNEGFFKTNPPILTGSDCEGAGELFQVESKSLSENKEKFFGKNAYLTVSSQLHLEVLSAALGKVWTLSPTFRAEASGTNRHLSEFWMLEAEMPYVEDIGQITGLTEQMIKHVVKDLVNDTNQVASNLLKSQRSVEGQEGKTDLTEKWKKLYETEWKTITYKEALQILESSGETFQHPVKYEDGLATEHEKWLAGEYFKSPVFVIDYPKDMKAFYMKVNPDNETVACFDLLVPEVGEVIGGSLREHNLEKLTSIINDRGMKLDELKWYLSLRENSTVPHGGFGLGFERLICYLADVENVRDVVPFPRSKGECPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZH5</accession>
    <name>A0A1E3NZH5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_63151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037848.1">
        <property type="nucleotide sequence ID" value="XM_019183499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZH5"/>
    <dbReference type="GeneID" id="30200745"/>
    <dbReference type="OrthoDB" id="3982967at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06293">
        <property type="entry name" value="Kdo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="624" mass="71525" checksum="FE97D4D09A6CBE4F" modified="2017-01-18" version="1">MPFMGLEIDGYNPESPSNAQEDLRNSNTSPSTGSNNSLAHQIKSSNIKLSAEDTKENEFDSWLHERLETIDQDLMRNIQNACSADHTEVWRVLNCMWVCAGIPTEYDNREDSITQALHSCLINPVTGLFNIVYNMRLKARVNKPLKDAVELESPKFGEQIRSDLGVWSGPIGKAKIHLVLGAKKPFLSPLFNSKERGFDEKYRDFYCQLLSYLMISPTVRKGLLTDGVSMRFTHFNQIDIVNENMNSVQVDESSTNVNSSDFVDEKSKTIEMWKIYSQNASPEERVTASKIVMCILYDGCSGLINGSNAELERNDEDILTISSRCEFDTSTSPLLLQTSSGSSDTISSSRDQYSLTSTAARRNQFTNKPHTLESIPEAEIMEKIDNLGESIQEYSIDTPIEVYKMSAENYYLNFKPKNPVQGIPIQDELKDGNVILKLYDEEKIEKYFKEEGMSKYYTLTNESGGSPLFDEERYNSDFKAYEQSIFNRYLNEIQAYQRIQNYNETRLHPKHKINAPYLIGHGKIKQRSSGKFIYYMAISEIPFVERKPHDYDELLFSACDEIEKLHSIGIVHGDIMRCNICFDEQTSSIWIFDFNRCSLFDPARGIDQKLLNQDFIDLHDYKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZH9</accession>
    <name>A0A1E3NZH9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phospholipase D1</fullName>
            <ecNumber evidence="3">3.1.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Choline phosphatase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Phosphatidylcholine-hydrolyzing phospholipase D1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_95214</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phospholipase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="3"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037214.1">
        <property type="nucleotide sequence ID" value="XM_019186487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZH9"/>
    <dbReference type="STRING" id="683960.A0A1E3NZH9"/>
    <dbReference type="GeneID" id="30203733"/>
    <dbReference type="OrthoDB" id="14911at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09141">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000011">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF76">
        <property type="entry name" value="PHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="12">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="384"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="561"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="748"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="417"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="442"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ58007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="857" mass="98134" checksum="3D3B44FC1A3920A4" modified="2017-01-18" version="1">MYDRDTVPRMPWHDVHMVTAGQPAADLGRHFVQRWNYLLRQKRPSRPTPILTPPSEFSPDNLERFNLTGKCEVQLLRSSGNWSLGLKKTEHSIQNAYLKLIETSEHFVYLENQFFVTASAFDGTVIENRIGDALVDRIIRAHNEKKKWKAVIMIPLMPGFESQVDEAEGSAVRVIMQCQYMSISRGSTSIFAKLKKMKINPYDYIQFFSLRKWGRIGPNRKIVSEQLYIHGKTMIVDDRVAIIGSANINERSQRGNRDSEVAACVRDTETVNTTMNGKPYKAARFAHTLRIRLMREHLGIDVDILEIVEKRFNKLEELARSTKVGQDSKTHNFNQKENEVTSSMVELASRDVLNLPNGTKKWQTFKKQAFGDANAAPKYDENYKDIDQQDNGQNLPPHLKEKDHKPAPLFYHSFNNRAREENTGIRDKKQFSTDSRVSNTTKHKDDIEGKGQDRMESKEYKAYKLSAAKTLKRWAKESLVNDTTDVFLPNVEQVLEFLENDDFIDNPSSLSDKEEFVLAERNQERWDLLKRIAYLQRVAAKQKVQNDDENEKRAKVGISSLDNTFTRSDHNGKTSAVDKVSKPKASQGADPKDIEANAGVDARPSTELQEVENLPGDNIPVVALDSQGIRDVLDSLSDSKQGDESKFNKFIDPYCFSDPIDDAFYEDVWFENALRNSKIFREVFHCQPDDAVTTWKEYKEFSRLSTAFGLAQEQEVHRREALFKSMSHSSNGEDEAETLANVRDRMERHNSVSAQDLDDTDGVLGKPPGRQTQNTAVDEGEDPDDQATYPRRGKSGAYGLRNKLMGEKVFDRETADRLLSEIQGHLVLFPVDWLYKEVESGNWFYNMDRLPPIDIYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P012</accession>
    <name>A0A1E3P012_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nitrogen regulatory protein areA GATA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_63114</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037811.1">
        <property type="nucleotide sequence ID" value="XM_019183492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P012"/>
    <dbReference type="STRING" id="683960.A0A1E3P012"/>
    <dbReference type="GeneID" id="30200738"/>
    <dbReference type="OrthoDB" id="5563539at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007039">
        <property type="term" value="P:protein catabolic process in the vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013860">
        <property type="entry name" value="AreA_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052292">
        <property type="entry name" value="Glucose_repression_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28051">
        <property type="entry name" value="PROTEIN MTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28051:SF1">
        <property type="entry name" value="PROTEIN MTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08550">
        <property type="entry name" value="GATA_AreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitrogen regulatory protein areA GATA-like" evidence="2">
        <location>
            <begin position="226"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="391"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="468"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="634"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="751"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="121"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="423"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="470"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="643"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="671"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="751"/>
            <end position="780"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="832" mass="93622" checksum="3D5F146D4ECA6AF2" modified="2017-01-18" version="1">MPSTNNLAQYFAQQQQKSSSNDSNSVPRPSINIPRSSKSPSPSPSSYSVHSKAVDAEDDDHFEKSTFQLKRTRSVGLLDEFIAPTRKLMEADQQVDGDETEEEEEEDNEEDGKYDIDYDDTNNNTSRNFNDNNNDNDDDENQFIEDVDDESFKSKPKNKKSPVYSYDDDYSYSSFDNEDENASMSPSPPETPDLYQPHDDNDIVYEPQRHVDYLSHNWKESDIQKSWRYIVLKRKDVANSARLENASWRTWAQAKYHLKTISPESVNWLKDSDVTWLYGPLYKEPKNFQALDGTSSSNATDKKYGSDSESAPITKKSIKQVPGSQPRIKGPKPILKKRTVSEMLSQPSLIKLPPHRHRGQSPIIESGHSLPSPPSIEDDFDVISSRVNAQYKKHPHHEESNQQQPHHDQQKNILASASSLDSIPKRHDDSKEQIASSSATPQLQPQQPPKRHIHFNDRVEQCMAVNYPDSGEDEEYDEDYEGDEELNESDSEDEEGGFFLQVRAPSTPQIHNKDLVLPLSLNEAKNLTQGEHPPRFQTIEILPCTTLKHGSDEEDEEDDNDDKYAVSHNVNTSRGFNYYYDYNSVYPSDQSIPHVHLGNNEYELDASAQHLEGVKLVDVPENIILGSNVEIEDTPKFAEPETTESIEQSTILQKPENKSNTSGAFVLHSDEEGDSSSEEDEEHDNDQQSMNPNTSSSSSSGGSKNFGENIGKISSSPSYASLSDVAAQGYISQSPNSNNLSNVISKNYNNANNNIKSNEPVINHNNNNSNSNDDNKIPSNAITSEYGNDDNDNNNFNKSDVNEVQKESGSASPSEEKTGFKHTFLNSWKRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0C0</accession>
    <name>A0A1E3P0C0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ras-GEF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_32666</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038096.1">
        <property type="nucleotide sequence ID" value="XM_019181992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0C0"/>
    <dbReference type="STRING" id="683960.A0A1E3P0C0"/>
    <dbReference type="GeneID" id="30199238"/>
    <dbReference type="OrthoDB" id="546434at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF354">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="93"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="8">
        <location>
            <begin position="586"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="7">
        <location>
            <begin position="867"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="42"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="59"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1144" mass="131358" checksum="69BE79ADD75D2F37" modified="2017-01-18" version="1">MKSRSLSTNNDSVDSPTSSGLQDQSFNYSFNDTTYSKESEYLNDLKKEKKEKQEKHNTANLLSGSAAGADSISSNPQDRKSLRYSSTPEDEDLVSLFLSALHGFDANSLELTSDSAICLSFEKNDVAFVHSVDSSGWGEVTMIKNFKRGWVPINYFTELVKPSDQPLPSAKSKSPLKLLLISSAKFLRSPAEDDGKSGFSINHINDIRDGVRYLLEKTDCLSRSTDIVKKKPIIRRIRKSLLADWYSLMIKADSYKHSTSVVHLETLQLMVLQVVRKSLAFLEIWGIENDLLQKENQERQLKAKAEKKNNIPYLGEQPYAKERLNEIHNLLFTYIGLILGRLDMIEHNPAGCQLLENLIHQMILLLREILFISKSSVMILNNRSTKVETKVDDNLDTLLSLVSELVSSVKMFVTKTIHEDYGSLNLTVKDGLYYYSPEGDDLIGIISRMTRSISASIENCYRYITLIGDFRLGEEKRFPDFQAIKITPDSFIKTCSEGLLKTLDKSKVDLKELKRSNIKRQSRYSMVRSGLNNEMGLTSSGTNLLQEFLPDSKSFIRNSVFEPYLNESNEEIEIEHDAKSEIVYDSNGKLIGASFKGLVFLLTDELNKPDQFLISSFFLTFKLYANGVELIEELIGRFNITNKFDAFDDYDETGEYSSYESRLKNRRRIICKMFQIWLQSYWDYRNDYNLLPTLINFFNEGVSQFLPIESKKLIELCSKLTGLTPSKIKASPTVTTITPILAHSSSSATIANVPSIVSINDELDNDDYIFEEYELAKMNTSNRNSMLLPLPSINFNNNSLLSKSQIHEIENLVLQYRRFLGTTLWEHNDYTHYYPIDVNSLFDKWYTSCQTPFSQVIGIDYNLVELNSFEFAKQLTLIESRIFLSMKSEELLSGNFASKKSKLNLSPNVSRSLLFTNLLSEYVLDSILLPELPMKKRILRFKNWLNVALSSYYLKNFNSLAAIIISLQNHILSRLDEIWIGLPDKYKNLFKDLMKIIHPDNNYKGYRYKISKILDENETKSPIPLVPYINLFLQDLTFIDEGNRDFRNSNSFLRSKIINIDKFLKISKIVSNMEYLQVGYDNMGPTKKSKRNSIFSFNSGSSLNDNVSPILPLQEFILLEFWRVHQANLKHDDRYWVLSKQIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0C3</accession>
    <name>A0A1E3P0C3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Nicotinamide-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="16">2.7.7.1</ecNumber>
            <ecNumber evidence="16">2.7.7.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_55064</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate to form deamido-NAD(+) (NaAD). Key enzyme in both de novo and salvage pathways for NAD(+) biosynthesis. Predominantly acts in the salvage pathways via NMN.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 16">
            <text>beta-nicotinamide D-ribonucleotide + ATP + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 16">
            <text>nicotinate beta-D-ribonucleotide + ATP + H(+) = deamido-NAD(+) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22860"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="EC" id="2.7.7.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; NAD(+) biosynthesis; deamido-NAD(+) from nicotinate D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the eukaryotic NMN adenylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.1" evidence="16"/>
    <dbReference type="EC" id="2.7.7.18" evidence="16"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038060.1">
        <property type="nucleotide sequence ID" value="XM_019182752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0C3"/>
    <dbReference type="STRING" id="683960.A0A1E3P0C3"/>
    <dbReference type="GeneID" id="30199998"/>
    <dbReference type="OrthoDB" id="422187at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00332"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004515">
        <property type="term" value="F:nicotinate-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09286">
        <property type="entry name" value="NMNAT_Eukarya"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000074">
        <property type="entry name" value="Nicotinamide-nucleotide adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051182">
        <property type="entry name" value="Euk_NMN_adenylyltrnsfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005248">
        <property type="entry name" value="NadD/NMNAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045094">
        <property type="entry name" value="NMNAT_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00482">
        <property type="entry name" value="nicotinate (nicotinamide) nucleotide adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12039">
        <property type="entry name" value="NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12039:SF0">
        <property type="entry name" value="NICOTINAMIDE-NUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 16" id="KW-0520">NAD</keyword>
    <keyword evidence="9 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 16" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6 16" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="18">
        <location>
            <begin position="176"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ58853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="408" mass="46425" checksum="63485E852AEFDE9E" modified="2017-01-18" version="1">MDPKRAPDFRPPSTKPSNDPPLAPSSQIPKAAPIQPYVLADYPNKIDAPIKPRKHKHHHHHHHHHHHPSSSSQQPTSAPISGETKDLQPLSSAENSSTDEEDDEKSENIDALLEEPQEDPMKPTIKDGIQAYQTADLEEVPHGIVRQAKRIESYEFPTHRLIPKLKNPNKHPLVIVACGSFSPITYLHLRMFEMALDAIREQTRFEVVGGYYSPVSDNYKKRGLASSYHRVRMCELACERTSSWLMVDAWESLQPTYTRTAKVLDHFNEEINIKRGGILTSDGSKRGVKIMLLAGGDLIESMGEPNVWADDDLHHILGRYGCLIVERTGSDVRSFLLSHDIMYEHRRNVLVIKQLIYNDISSTKVRLFIRRGMSVQYLLPNSVIRYIQEHGLYINDPEPVKQVLDSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0I3</accession>
    <name>A0A1E3P0I3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_101649</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037639.1">
        <property type="nucleotide sequence ID" value="XM_019180329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0I3"/>
    <dbReference type="STRING" id="683960.A0A1E3P0I3"/>
    <dbReference type="GeneID" id="30197575"/>
    <dbReference type="OrthoDB" id="5286008at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045290">
        <property type="term" value="F:D-arabinose 1-dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070485">
        <property type="term" value="P:dehydro-D-arabinono-1,4-lactone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19164">
        <property type="entry name" value="AKR_ARA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044480">
        <property type="entry name" value="Ara2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686:SF1">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="26"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="334" mass="37555" checksum="9726FA7360C20D21" modified="2017-01-18" version="1">MTIADLKPIGPANVPVSGKIKDLPPLLLGGAVFNQQFNDNPENLPAEEMLKIAFSKGISAIDTSPYYGPSEIILGNALKNIKPEFPRENYYIVTKAGRVKLNDFDYRPESIRKSVLRSLERLDTDYLDLVYLHDVEFVSEDKILKALVELKNLKSQGIIRHFGISGYPVEFLYHIAKTVVNIPEIGPLDAILSYSNFNLQNEILRDYIDKFYNEAKLTKLLTGSILSMSLLRSGSTHEFHPASKELRDKCQEIAKLTASKGVELADLGTRYAIREFKDKGPVVLGVSNIQELTNAIEQYWNVVDNTIDDTELVTEVKEAFGEHLNETWESGIPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0J8</accession>
    <name>A0A1E3P0J8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histone-lysine N-methyltransferase, H3 lysine-79 specific</fullName>
            <ecNumber evidence="2">2.1.1.360</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Histone H3-K79 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_32839</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60328"/>
            <dbReference type="Rhea" id="RHEA-COMP:15549"/>
            <dbReference type="Rhea" id="RHEA-COMP:15552"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.360"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.360" evidence="2"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037997.1">
        <property type="nucleotide sequence ID" value="XM_019182010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0J8"/>
    <dbReference type="STRING" id="683960.A0A1E3P0J8"/>
    <dbReference type="GeneID" id="30199256"/>
    <dbReference type="OrthoDB" id="443402at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140956">
        <property type="term" value="F:histone H3K79 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000033">
        <property type="entry name" value="Histone-lysine N-methyltransferase, H3 lysine-79 specific"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021162">
        <property type="entry name" value="Dot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025789">
        <property type="entry name" value="DOT1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030445">
        <property type="entry name" value="H3-K79_meTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451">
        <property type="entry name" value="HISTONE H3 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451:SF0">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE, H3 LYSINE-79 SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08123">
        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017570">
        <property type="entry name" value="Histone_H3-K79_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51569">
        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DOT1" evidence="15">
        <location>
            <begin position="1"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="130"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="216"/>
            <end position="217"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR017570-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ58790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="343" mass="38951" checksum="7E4542E00CDF15F5" modified="2017-01-18" version="1" fragment="single">EPYYLMESRDFELDPLEEIGKLMESLSITYFPEEYKLTVYNPLDPYNSIAGRLSKGMDDADQKVILDSIKEYNELVTKIHQENGFIKHLKEKKTISRAFVHELLNQMYSRAVSPSVQRLRKYEAFSNNVYGELLPNFLSKAFDQVGLSSKSTFIDLGSGVGNVTIQAALEYGCESYGCEVMENASDLADLQHQVFNERCKLFGLNPGKVGFFNHQSFVDNPEVEAVVSRCDVILVNNFIFTPDLNREVIKLFRNLKAGTKIISLKNLVPAGHVVEFDDIENIINKFKVECFALDNGSVSWTDRGGIYFVSEIMGEINPDFLTVYRGRRSNPKAESEELVVKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0X6</accession>
    <name>A0A1E3P0X6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Aconitate hydratase, mitochondrial</fullName>
            <shortName evidence="14">Aconitase</shortName>
            <ecNumber evidence="14">4.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_84649</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="14">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.-" evidence="14"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038185.1">
        <property type="nucleotide sequence ID" value="XM_019185749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0X6"/>
    <dbReference type="STRING" id="683960.A0A1E3P0X6"/>
    <dbReference type="GeneID" id="30202995"/>
    <dbReference type="OrthoDB" id="2224430at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01584">
        <property type="entry name" value="AcnA_Mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000002">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000003">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000004">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1060.10:FF:000001">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1060.10">
        <property type="entry name" value="Aconitase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050926">
        <property type="entry name" value="Aconitase/IPM_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015932">
        <property type="entry name" value="Aconitase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006248">
        <property type="entry name" value="Aconitase_mito-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01340">
        <property type="entry name" value="aconitase_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005558">
        <property type="entry name" value="PRK07229.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160">
        <property type="entry name" value="ACONITATE HYDRATASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160:SF3">
        <property type="entry name" value="ACONITATE HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9 14" id="KW-0408">Iron</keyword>
    <keyword evidence="10 14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="15">
        <location>
            <begin position="65"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="16">
        <location>
            <begin position="582"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ58978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="780" mass="85184" checksum="143FB27614E69F15" modified="2017-01-18" version="1">MLAARNSLKAPRFARSLATVSSDLTRDSKVGQNLLEDFSFIQYKKHLENVEIVKNRLNRPLTYAEKILYGHLDDPHGQDIERGVSYLKLRPDRVACQDATAQMAILQFMSAGLPQVATPTTVHCDHLIQAQIGGAKDLARAIDINKEVYDFLATATAKYNIGFWKPGSGIIHQIVLENYAFPGALIIGTDSHTPNAGGLGQLAIGVGGADAVDVMADLPWELKAPKILGVKLTGKMNGWTSPKDIILKLAGITTVKGGTGKIVEYFGDGVDTFSCTGMGTICNMGAEIGATTSVFPFNKSMVDYLNATGRGKIAEFASLYKNDFLKADEGAEYDQVIEIDLNTLEPYVNGPFTPDLATPISKMKEVAIENDWPLDVKVGLIGSCTNSSYEDMSRSASIIKDAASHGIKAKSLFTVTPGSEQIRATIERDGQLKTFQEFGGIVLANACGPCIGQWDRQDIKKGDKNTIVSSYNRNFTSRNDGNPDTHAFVASPELTTAFAIAGDLRFNPITDKLKGADGKEFLLKPPTGDGLPTKGYDAGENTYQAPPADRSTVQVQISPESDRLQVLTPFKAWDGKDAENLPILIKSKGKTTTDHISMAGPWLKYRGHLENISNNYMIGAINAENDKANCVKNLYTGEYKGVPDTARDYRDQGIRWVVIGGENFGEGSSREHAALEPRFLGGFAIITKSFARIHETNLKKQGLLPLNFVEPEAYDRINPEDTVDILGLTELTPGKNVTLRIHPKDGEAWETPLSHTYNSEQIEWFKYGSALNKMGFVNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P111</accession>
    <name>A0A1E3P111_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_79486</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038147.1">
        <property type="nucleotide sequence ID" value="XM_019184902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P111"/>
    <dbReference type="GeneID" id="30202148"/>
    <dbReference type="OrthoDB" id="4092221at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="549"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="570" mass="64993" checksum="AD1E1A9E800F9A0F" modified="2017-01-18" version="1">MIPWDNLKVDSGEVENQQMQFIERRGIPGVTSDEYHLGYFESSSSYKSRPPVHTKFDPLYETLCFFGVLIFGALIVTCITFGIREYTYSMRRRNRLFSAGSEWSINTAITIPEFSNSMIQSAILGPQGNHIYPNLLKNQQTNQSHLFSPSANSEVDETLFTDARETAKGLEGPSDFTPLEVVQQSPNYSSKSLEGFFSDNDLLKTSNVTKKIYSIKELFSLKPEDYHGRPLTKDGPVELTTLAKAVSLSIPLQTVSSQSAWTAEKTLLEICLYLTNEMIPPQPVHDNATKKIAVLRFLAIAANCSSLQNPNIFLSPLNRVMDALIDSGIIFEILNDSSPYISRSLIEVLLQYCFAHWHFPAREKNDHIIHKQFAKWKMNSPAVQPHHVLFRYLINLELGLYSVDNLDSAMLKAIESRIITLLIHLTGNADCNDYLGFIPILSQAIEFTERTYCQTLCYDLLHILVQKHSDCCMEEYLKDDKSDLKRIIQCGFWIKNDRLIRKFTERIHVIVLSKNPKAMSWWKEINGGRIYISPSPRELDLKCDTSDIRNSQRSITPGSWVNDRKPKTPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P126</accession>
    <name>A0A1E3P126_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Knr4/Smi1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_20710</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KNR4/SMI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038240.1">
        <property type="nucleotide sequence ID" value="XM_019180882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P126"/>
    <dbReference type="STRING" id="683960.A0A1E3P126"/>
    <dbReference type="GeneID" id="30198128"/>
    <dbReference type="OrthoDB" id="2305498at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070880">
        <property type="term" value="P:fungal-type cell wall beta-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032995">
        <property type="term" value="P:regulation of fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009203">
        <property type="entry name" value="Knr4/Smi1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018958">
        <property type="entry name" value="Knr4/Smi1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037883">
        <property type="entry name" value="Knr4/Smi1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051873">
        <property type="entry name" value="KNR4/SMI1_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47432">
        <property type="entry name" value="CELL WALL ASSEMBLY REGULATOR SMI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47432:SF1">
        <property type="entry name" value="CELL WALL ASSEMBLY REGULATOR SMI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09346">
        <property type="entry name" value="SMI1_KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017023">
        <property type="entry name" value="KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00860">
        <property type="entry name" value="SMI1_KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160631">
        <property type="entry name" value="SMI1/KNR4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Knr4/Smi1-like" evidence="3">
        <location>
            <begin position="98"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="33"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="186"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="359" mass="39845" checksum="421DAA760F64002C" modified="2017-01-18" version="1" fragment="single">MGFKEKLNQIFYALSTEDRYADYDSHNHHIRTAGNQEESASPAGIQLNDADGFQDDGGDDSGLHHSTEGVSETILAWRHIETWTSENHTDLNASLSDPCTRTDITKAEQDLGIALPPPVRASLRIHDGQEDLDSLTGIGGLFFGLQLMNLDEIVHMTKTWRLVADRINLDIEVKKNKAATESLISPAGSSTEVDKTKSTTSLNETHKGFKPVEGTDYKNLDPDLERKISSNNSKRFKSHIPKQFSCPPGAIHAVYAHSGWVPLVTDNSGNHIGVDLAPGKKGTWGQVILFGRDFDTKFVLAPNWGDFLLIFAKDLENGNYIIREEDDVFAGEGEVAFFDKKINREVPYLQVLAQRVINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P194</accession>
    <name>A0A1E3P194_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_79635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038310.1">
        <property type="nucleotide sequence ID" value="XM_019184925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P194"/>
    <dbReference type="STRING" id="683960.A0A1E3P194"/>
    <dbReference type="GeneID" id="30202171"/>
    <dbReference type="OrthoDB" id="3546279at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053157">
        <property type="entry name" value="Sterol_Uptake_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784:SF5">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="1">
        <location>
            <begin position="19"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="457" mass="53305" checksum="6CE023AC8D787983" modified="2017-01-18" version="1">MSDTNQITKRYKTKRSKNGCLSCKKLKIKCTEDKPTCEYCLHTNRECVYLNKPSFVKLKVVKTKQPPTSLNHDTVLKLNSTTNHLQISNFELKLMKFYLEFGADFFSFKVNLDNYKFWNEKIPNLWCCSDLIKLSIYSLSSARLLASYNESNSFTSVYLECGDDEDQEKTINLYDQVQHFKNKTIEMMAYSNFMIDSQIDSQEMIDIGGQLLVAKFIMTAVFAMLPDEDNDMNIVELNKFKIFKIFETTKDYQKILSNNFNLLQNSKYMKILPPTFAYVIDNSNDGFKFTQHLRSYILEKTNPLDMLQVSYLNFISSIESNCVKAIELNFPFALFLPISEISLDDDLILSLKQGGNHLALKLLFYICCIISIFDFKLARTGGVWNQVIEFYRKQCFHKFNNEFEDEFDQNVYDLVLARRVYKIPYDFNLIYDLGEPVQDFINGKIKLSPNEGPSVFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1D6</accession>
    <name>A0A1E3P1D6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_31761</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ59060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019181878.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3P1D6"/>
    <dbReference type="GeneID" id="30199124"/>
    <dbReference type="OrthoDB" id="2102561at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000140">
        <property type="term" value="F:acylglycerone-phosphate reductase (NADP+) activity"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05374">
        <property type="entry name" value="17beta-HSD-like_SDR_c"/>
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    </dbReference>
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        <property type="entry name" value="NADPH-dependent 1-acyldihydroxyacetone phosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
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        <property type="entry name" value="SDR_fam"/>
    </dbReference>
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        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169:SF6">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
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    </dbReference>
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        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GDHRDH"/>
    </dbReference>
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        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ59060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="292" mass="32787" checksum="8D92C6EDFE4B0275" modified="2017-01-18" version="1">MTRQKTALVTGASSGIGYAVSKELALKGYKVFAVARRTEPMESLKQYGVIPKSLDVTSQESVQLFKKYLLETLEDGKLDILYNNAGQSCTLPAIDVTDDQVEQVFQVNVFACIRLTRELSPFVIKAKGTILFTGSLAGIAQFPFGSVYSATKGAIHQYARVLHLEMKPLGVRVINVITGGVNTNIADTRKIPENSLYNTPEGAESFEMRQRLAKENTPMEASVYAKSIVKNIQSSRDPIDIYRGTYSTMVPFLIRWFPGWFVELVFIRRFKLGRMFDSMKKKNTDEFNLHLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1N1</accession>
    <name>A0A1E3P1N1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_94554</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="30203698"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="71" mass="8176" checksum="7A50963F57607826" modified="2017-01-18" version="1" fragment="single">MVIIIIIDDVNLIYSINNVKQKNLSRMQALINFPRLVAKQCQINNRKQGSGNFTRKAILLLKKDCTTKFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1Q8</accession>
    <name>A0A1E3P1Q8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_63770</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="30200853"/>
    <dbReference type="OrthoDB" id="3979747at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="404" mass="47352" checksum="D2F827E0B4453232" modified="2017-01-18" version="1">MIDSLPNEVLRQIVRQLDNKTLDHSISQIPVLRKLSTLKITDNDEIMVNEDTSDVDVITIQSIINHKLSLDSIFKQYHFYQIEIHNFSKDESIYMRFHKLLARLIRLKKIISIKYLGAEIDMNYLKMFKSLFNNHIDPSSTHPVSWFDITFENLKQVKSNCNHLLISPNVSYPFLESLNLYYCSPKFYFEILNYLDLQKFPSTFPNVTYLKLQDYMNEEEDIYSQHSNNYIKSRDDDDDGDNFDDYDDYDNNSDLDENEFYFDGLEIIQNYNLSKLQVLIIKNCNVSKIKKINAPNLDTFKIFELSNKHIDAYDLNLIDSSNIVKRSFISSAVSTSCCLQQNNFPNLRTLKIIGHLKLTDFSKNILDLDEIQQIIIKGCNVEIIDKDSFWDSYHGNNQIALEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2H4</accession>
    <name>A0A1E3P2H4_WICAA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_79919</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038611.1">
        <property type="nucleotide sequence ID" value="XM_019184977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2H4"/>
    <dbReference type="STRING" id="683960.A0A1E3P2H4"/>
    <dbReference type="GeneID" id="30202223"/>
    <dbReference type="OrthoDB" id="428895at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035426">
        <property type="entry name" value="Gemin2/Brr1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04938">
        <property type="entry name" value="SIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="237" mass="27113" checksum="0FD0AAD2961DEC1F" modified="2017-01-18" version="1">MGNKRSGNSQNRNNKRRRNGDDDEGSNSHQLDPVFGQYRALPISQEKLANVKPDTIPEDAETYLAMVRRQAETGSSVMFISKEINYDDDGEEQTKVSTAIQINSKTDEEIEYERSILEKFQRERESLPQAGELDIPSNFVFPENFTEWRRFVMQNQPTVSLISNFDHELTISTWIFALLVKLNDVLESSDSATVRELAQRAKKINAKGDVVSETTRATNDFVIIIVSRFFGQRDLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2J9</accession>
    <name>A0A1E3P2J9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Clp R domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_33284</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038687.1">
        <property type="nucleotide sequence ID" value="XM_019182053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2J9"/>
    <dbReference type="STRING" id="683960.A0A1E3P2J9"/>
    <dbReference type="GeneID" id="30199299"/>
    <dbReference type="OrthoDB" id="47330at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19499">
        <property type="entry name" value="RecA-like_ClpB_Hsp104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000017">
        <property type="entry name" value="ATP-dependent chaperone ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000120">
        <property type="entry name" value="ATP-dependent chaperone ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000025">
        <property type="entry name" value="ATP-dependent Clp protease subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000010">
        <property type="entry name" value="Chaperone clpB 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050130">
        <property type="entry name" value="ClpA_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638:SF176">
        <property type="entry name" value="HEAT SHOCK PROTEIN 78, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="52"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="449"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="Clp ATPase C-terminal" evidence="9">
        <location>
            <begin position="623"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="264"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="726" mass="81915" checksum="94000A8B7F1062C9" modified="2017-01-18" version="1">MNMNQGQQEPEKPALEQYGTNLTKLAEQGKLDPVIGRDQEIRRAIQILSRRTKNNPVLIGRAGVGKTSLIDGLAQRIVKGEVPESLKNKQLVALDLGALIAGAKFRGEFEERLKSVLEEIDKANGDIIVFIDEVHMLLGLGKTDGSMDASNILKPKLARGLRCISATTLDEYKIIEKDPALSRRFQPIQLNEPSVEDTISILRGLKERYEVHHGVRINDSSLVSAAVLSNRYITDRFLPDKAIDLVDEACAVLRLQHESKPDSIQILDRSIMTIQIELESLKKETDPLSVERREKLEEDLKLKQDELSRLTEVWEREKAEIDAIKNAKKDLEQAKVDLEIAQREGNYGKASELRYSRIPDLQKKVELSEQEENKPDSLLHESVTSEDISKVISKMTGIPIDTVVKGDKDRLLYMEDSLKRKVVGQDEAIHAISDAVRLQRAGLTSDKRPIASFMFLGPTGTGKTELTKSLAEFLFDDSSNVIRFDMSEFQEKHTVSRLIGAPPGYVMSESGGQLTEAVRRKPYSVVLFDEFEKAHPDVCKVLLQVLDEGKLTDSQGTQVDFRNTIIVMTSNIGQDILLHDEEIKNDTGKVSQSTKNKVIEAMKHNYPPEFINRIDDVLVFNRLSKEVLRNIVDIRLNEVQERLVDKRIVLDLSNEAKDWLTEHGYDNLYGARPLNRLIQKRLLNPMAMYLLKGQIGNNETVKVVVKDGDIVVEPNHNEGEVVEEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2K6</accession>
    <name>A0A1E3P2K6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_91311</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038790.1">
        <property type="nucleotide sequence ID" value="XM_019186284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2K6"/>
    <dbReference type="GeneID" id="30203530"/>
    <dbReference type="OrthoDB" id="10621330at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="374" mass="43053" checksum="C6CA1D853AD5FF4F" modified="2017-01-18" version="1">MTDGDSSFYGKLHKSDLGKSKHQKNTQSDNEWIILLLQSLFNQLPLKSIDTVEIVAKELSKDGETILEIEVKTKVGPSIYQTLGLLEFKALAESDNINYADELFEWSKIMSSTNKQLQKELVKTKARTKSLEVELKEMQKFQDDLVEESKVKDEIQLKVMTKLLNSKKEHYEKLLKGEITDDPDDFNLIAIKNIKDDFIHEQEKEEKPKPKKPRKAGAKRQPRGVKAAALLRGEKNITKEYPRIKDEDEDEFIPDSQDEAEVKKENDPVEKQMTDEPKKKKVKIEPKDSKIKKEQDEIPQAVKAEPEKSIHFKFNDEDATEDDEEVVEEESKTKDDEGDETVEDTDEDGTEEDLEMKDQDQDQETATDTDSDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2Z7</accession>
    <name>A0A1E3P2Z7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methylthioribulose-1-phosphate dehydratase</fullName>
            <shortName evidence="7">MTRu-1-P dehydratase</shortName>
            <ecNumber evidence="7">4.2.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MDE1</name>
        <name evidence="9" type="ORF">WICANDRAFT_83842</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5-(methylsulfanyl)-D-ribulose 1-phosphate = 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:15549"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="ChEBI" id="CHEBI:58828"/>
            <dbReference type="EC" id="4.2.1.109"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 2/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the aldolase class II family. MtnB subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.109" evidence="7"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038992.1">
        <property type="nucleotide sequence ID" value="XM_019185607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2Z7"/>
    <dbReference type="STRING" id="683960.A0A1E3P2Z7"/>
    <dbReference type="GeneID" id="30202853"/>
    <dbReference type="OrthoDB" id="191080at2759"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00875"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046570">
        <property type="term" value="F:methylthioribulose 1-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000003">
        <property type="entry name" value="Methylthioribulose-1-phosphate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03116">
        <property type="entry name" value="Salvage_MtnB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017714">
        <property type="entry name" value="MethylthioRu-1-P_deHdtase_MtnB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027514">
        <property type="entry name" value="Salvage_MtnB_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03328">
        <property type="entry name" value="salvage_mtnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10640">
        <property type="entry name" value="METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10640:SF7">
        <property type="entry name" value="METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="8">
        <location>
            <begin position="18"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="237" mass="26591" checksum="D0194B80AE5F87E4" modified="2017-01-18" version="1">MSQYDQLITSSDPSHPANLICELCKLFYNNGWVTGTGGGISIRDGDKIYIAPSGVQKERMVPENMFVMDFTTTKYLRTPKVFKPSACTPLFMSAYTMRDAGACIHTHSQAAVLVSLLYEKEFKIANIEQIKAIPKVTEPGYLSFFDTLTIPIIENTAHEEDLTETLQQAIKNYPGTTAVIVRRHGIYVWGETVWKAKVYNEAIDYLLELAVKLKGFGIPPHIGVGEEKNYVDPKIYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P368</accession>
    <name>A0A1E3P368_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TATA element modulatory factor 1 TATA binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_62239</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038861.1">
        <property type="nucleotide sequence ID" value="XM_019183336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P368"/>
    <dbReference type="STRING" id="683960.A0A1E3P368"/>
    <dbReference type="GeneID" id="30200582"/>
    <dbReference type="OrthoDB" id="74178at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052602">
        <property type="entry name" value="Growth_transcription_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022092">
        <property type="entry name" value="TMF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022091">
        <property type="entry name" value="TMF_TATA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46515:SF1">
        <property type="entry name" value="TATA ELEMENT MODULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46515">
        <property type="entry name" value="TATA ELEMENT MODULATORY FACTOR TMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12329">
        <property type="entry name" value="TMF_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12325">
        <property type="entry name" value="TMF_TATA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA element modulatory factor 1 TATA binding" evidence="6">
        <location>
            <begin position="690"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="144"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="600"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="656"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="227"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="490"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="701"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="60"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="600"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="671"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="808" mass="91785" checksum="1A152309762E516B" modified="2017-01-18" version="1">MAKKKNNKSKSAANKPLQDTVTKPQQDTISNPEPAIDENDDNNTTSKTVQEPTNDISQQEQKDIETIQEKDQVTKPVESPSLINSAEVETEKSPEPIEVSESIKIEPQQEDEPIVKEQPIEIVQEPIAPTVKKRLTLQERLALAAKSGKSKRSTSGANATDDKKLRVVSPEPSTRETSIEPQTSELSKVATPISEPISAVSTRDSSLDIKRSEPITLFPKDFKNLPVEELQKLITDAETKFSVLESKNESLTIEKQSLQEKIKNISSSISATSSSSASAQIIKEKDEKIAQLLKEGENLSMKELKYTNTIKRLKSIEADNDKEIWNLKNTVDSLEDEKKKFKEENKKLKDSEQQFSVKSKTLQIQLDNEKESLQEEINRNKELSKKIEELSQVLIQEKNQASNIINELKRSLEKEKQKAQTAQEESMAEINRLESKIEQLRYQSENAQAHTTTNDESYLKLIRQHDTLQNQYTSATENWQSIEASLMSKISNFETSLSSYQEKLKSSEEKNDILSNDLRSKTLEIENLTKDLKNLRLELSRCQNKLDQSTKDYEELQTTLESKEISFNKEKAELEYKVSNLERQFKDSIQPQHLTVQSSSNLGEFSPQMPQNSSTPSFRRGLSWDIGLGESSTTPRQSRKSSALYFPNYSQANGSFEEKSETASDFDDNDTVNSPVSQYHNGSRYDNNSSSALGGTSVQLLGKMSSQVRRLETELSTMKEELEKLSEDKKAANEEIVKLMKDNENVGFYKSKIEELEIQVDNYSKRHEKTLEILGEKSEQVEELKADVQDLKDLCRQQVQQLVDLQTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3C6</accession>
    <name>A0A1E3P3C6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exocyst complex component SEC15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_62438</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the SEC15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039060.1">
        <property type="nucleotide sequence ID" value="XM_019183366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3C6"/>
    <dbReference type="STRING" id="683960.A0A1E3P3C6"/>
    <dbReference type="GeneID" id="30200612"/>
    <dbReference type="OrthoDB" id="10267033at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.670">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.30">
        <property type="entry name" value="Exocyst complex subunit Sec15 C-terminal domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007225">
        <property type="entry name" value="EXOC6/Sec15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046361">
        <property type="entry name" value="EXOC6/Sec15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042045">
        <property type="entry name" value="EXOC6/Sec15_C_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048359">
        <property type="entry name" value="EXOC6_Sec15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042044">
        <property type="entry name" value="EXOC6PINT-1/Sec15/Tip20_C_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702">
        <property type="entry name" value="SEC15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20651">
        <property type="entry name" value="EXOC6_Sec15_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04091">
        <property type="entry name" value="Sec15_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025007">
        <property type="entry name" value="Sec15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC6/Sec15 N-terminal" evidence="7">
        <location>
            <begin position="54"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex subunit EXOC6/Sec15 C-terminal" evidence="6">
        <location>
            <begin position="415"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR025007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="792" mass="92735" checksum="B00E78CAFD95CA37" modified="2017-01-18" version="1">MSKVNDSSNFKQLSFLNESDPITLLNSNNYLDQLVPIIKSNLKSNTIPELLETLNTEGLKKDDELTSNINQNESTIRTTTSDISQISRNASTINEQILDINDHLAKTSNLTIQKKFQMLGLKKNINKINESVILINKILQILELTDRTHELIKNNKFFNALKNLNDLNALNKDFDKDFKFLNNINDSIPIMKELIRDESLSLVKRNLNSLDAKYDTISLAYYERFGHLISEWDNFKHHNRDFDKYKINSSVEISMRDSYASPESHLPHAEDFLNIGFIYDSLLIFKSLDQLQFLKHEFNKELNLRRDKILYPFLTNDMHNEQFKKYIVTNDNLKSFLARLIGFLIFHTLISEKLPNIVIQKTNDLWENLSAKIYPFLKDLVSNGLLEISRIIDFKTIIGNFYLILEHFNLNGDHFYNLLIITFKKYSQVATFNFKSEFQKLAEDDDSMPMVIYDLKLYKKIINLSWYEENRPEKEIEFPQVLPFSTIYPMTCAQVRVFINQQTQFLKDFYKYDSDSLNKLVVENVDNLLLTVVNKHFHEKLNLITREELSQNLINLEYFLIMSKEVLKQLNNTLHRNDQLKSIQAISETRKITETKLFEMVDGKIEDLIDFIDWDWQTEDFNNEPNFFIKDIGDFLKNMFNSTFSNLPYSVKTLLLYRVFDLLAIRFLENLNEQSSVSKASVSNFDTDISYMEAITQELNPSKGTSDGTSRESLQSMFLQLRQTINLLKSGHLDEYKDQTTRMRKFDQIKPDDAVNLIKKITPESRPQTPIDSPDPSLASKYSKFYKFRSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3I5</accession>
    <name>A0A1E3P3I5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">AdoMet-dependent rRNA methyltransferase SPB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_94855</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038669.1">
        <property type="nucleotide sequence ID" value="XM_019186467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3I5"/>
    <dbReference type="STRING" id="683960.A0A1E3P3I5"/>
    <dbReference type="GeneID" id="30203713"/>
    <dbReference type="OrthoDB" id="1287559at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012920">
        <property type="entry name" value="rRNA_MeTfrase_SPB1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024576">
        <property type="entry name" value="rRNA_MeTfrase_Spb1_DUF3381"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF13">
        <property type="entry name" value="PRE-RRNA 2'-O-RIBOSE RNA METHYLTRANSFERASE FTSJ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11861">
        <property type="entry name" value="DUF3381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07780">
        <property type="entry name" value="Spb1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="7">
        <location>
            <begin position="2"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3381" evidence="9">
        <location>
            <begin position="164"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA methyltransferase SPB1-like C-terminal" evidence="8">
        <location>
            <begin position="530"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="366"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="494"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="648"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="373"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="400"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="447"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="521"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="542"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="750" mass="86310" checksum="F16584445976F62F" modified="2017-01-18" version="1">MIIGVDIVPIKPMPNCITFQSDITTEDCRSRLRGYMKTWKADTVLHDGAPNVGLGWVQDAFTQSQLTLQALKLAVENLAPGGTFVTKIFRSKDYNKLMWVFQQLFEKVEATKPPASRNVSAEIFVVCRTFKAPKKLDPRLLDPKEVFEELADGPQNNEAKVFNPEKKKRSRQGYEEGNYILHNTMPIMEFVRDENPIDILGETSEFIIDQEDPDWKLVRKLKQTTNELLECFKDLRVLGKKDFRMILRWRKIARKLLDLDKEEEEIKVEVEPLNEEEQIDKELDQVRSRQLQKQKREKRQKNEQKQKEIVRMQMNMLTPKDIGIEAASIGADSLFNLKTAEKTGQLDDISRGKRRMIFDQNELYQGNDFQFGSDDEKEIGSEDEVDELEAQLDNMYAAYQERRSERDAKFRAKKARGDEDDEAWDGIKDDAKSDEEEEEATTTKDYNEDDEDISDSDDDQHINNLIAKLKPQNTKSGLSKKADLLFSNSIFDGVESEIPKVQEPTPIASSNNEESTTASLESDDSDEEMDSDSDFEVVARESEDEDDFNDSDDDDDVNLRNHQKDVDIATAQAMTLAHQLALGQRTKHDLVDDGFNRYAFRDQEGLPDWFIDDEKRHSKISKPITKEAALAIKEKMKQLNARPIKKVAEAKGRKKMRAMARLEKLKKKSDIINEDSDKSERDKAEEISKLMKKLTKKQKTKPQVTYVFAKGNNRGLSGRPKGVKGKYKMVDGVMKNEQRALKRIAKKHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3L3</accession>
    <name>A0A1E3P3L3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_92106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039212.1">
        <property type="nucleotide sequence ID" value="XM_019186338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3L3"/>
    <dbReference type="GeneID" id="30203584"/>
    <dbReference type="OrthoDB" id="3980139at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF12">
        <property type="entry name" value="REGULATORY PROTEIN CAT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="622" mass="72007" checksum="D563A6E03D25CFD7" modified="2017-01-18" version="1">MFDNFIEKENYVGLNCLNLLFNKMLKNFQLSPMEELKFPTIDFNNLNDFIITKFLIKFPSKTNLDLLISNHFENLNLIPILDEDLFFKNYKDIFNSIDNKTENLIHLLENNSHAGQEEIIFFIARLVLIIQLNCSIFNLHEIHKLITSLNLSINCSISKFQTTLLALYLFHNKNCYKDTVINLTNTSHSMVLNLGLHLNYNNLKQNCSDINLTKEERFKLHSVRLKLYWSFYILSTISSISFGLPQVEFFEKFEIPQLTSILNSNQNLKNCILLIDLLKNLENVNLLSVNQDLYMFKKIDEDLVNWRKDLKLQNLFHKIPMGSILSDPDSDKRPTKRLKKSKILSSHEENTKIQLNLFYIYFRMMIHLQNSYDDSIPSNISQSDYVLSNLSREFLAYIILLDSRKELNNLNNFEFHLIPINFLKMTLISMLNLTKISRDHFNKNAALFNDSNHILIRVLKIIKNRYPENFILFKQIVDFIKSEFQLFDSELDSSSPSINNLEINQTINNSTIASNGPPSTSSSSSGIDHNSLFFDIDSLNRNSSIASTESTISTTSAESISLDYNDTDSSTINDHLPDISSNLDLIFDWNSTTNPNIMKPLPNLQDPTIINGFNGSFDFKLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3S8</accession>
    <name>A0A1E3P3S8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_29623</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039210.1">
        <property type="nucleotide sequence ID" value="XM_019181632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3S8"/>
    <dbReference type="STRING" id="683960.A0A1E3P3S8"/>
    <dbReference type="GeneID" id="30198878"/>
    <dbReference type="OrthoDB" id="10260285at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007133">
        <property type="entry name" value="RNA_pol_II-assoc_Paf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23188">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23188:SF12">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03985">
        <property type="entry name" value="Paf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="129"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="378" mass="43055" checksum="E32742013FEE8ED6" modified="2017-01-18" version="1">MSKSQDYIARIRYQNDLPPPPLPPNLLNYKIPKDEEIGSSSLLSSLYRKENVNNLIKLNDDLGQSIDLIQVPDAFDRSKQDSKLYALSDNIKLHPNDRILLRDPGVDTVVGKQPNVAFLRRTEYIGSSRQNANATVQNSRLGSPQVSQDDNTPATQLRSIESTFTNSTKTLKNLTLLKHPLKKNLKAKKVWSLLPDTSRMDQSFSSIRMLGSASTSNRGTTSTEFHTSIFRPVELEQADWMSFYVTDEESSTSVKRTIDDLSENVPNDEIDENEGSRYKYLKKNDYDMKAIAVEGGIKDIALRFDHKENIAYYNPIQSKAELKRHRLHDSLKELVEQVDYDEVNLKIREPTNAELNSRNSIRHSHDPVNYEAVEVDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3X6</accession>
    <name>A0A1E3P3X6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondrial distribution and morphology protein 35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_62516</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRIAP1/MDM35 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039147.1">
        <property type="nucleotide sequence ID" value="XM_019183386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3X6"/>
    <dbReference type="STRING" id="683960.A0A1E3P3X6"/>
    <dbReference type="GeneID" id="30200632"/>
    <dbReference type="OrthoDB" id="19091at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990050">
        <property type="term" value="F:phosphatidic acid transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007918">
        <property type="entry name" value="MDM35_apoptosis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46403">
        <property type="entry name" value="TP53-REGULATED INHIBITOR OF APOPTOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46403:SF1">
        <property type="entry name" value="TP53-REGULATED INHIBITOR OF APOPTOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05254">
        <property type="entry name" value="UPF0203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="89" mass="10218" checksum="29E7538D87591752" modified="2017-01-18" version="1">MGNAMSSSFAPECTAAKKEYDDCFNNWYTEKFLKGKSLYNECEDLWYDYKNCVDLALAKKGVLPMLEEAREEAPFEKGGVLQDVKDEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P417</accession>
    <name>A0A1E3P417_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_78279</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038849.1">
        <property type="nucleotide sequence ID" value="XM_019184695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P417"/>
    <dbReference type="GeneID" id="30201941"/>
    <dbReference type="OrthoDB" id="10622678at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="296"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="314" mass="36676" checksum="1694347C2D7789CF" modified="2017-01-18" version="1">MTTQREYTPPPLPKSAPIQSELTNSAKSKRIPPFYKSHSKDSSSQKHKQLPELQTTTPSRSNNTQFAILPSIKLTPVGSDSYRHIKSASYDEKANGKKRLVDQFYDQLGTPPSSSTRLNTPVLTRNNTTTSFANESRSNLVLNKFEFDKKDVEVEQHNNGNIVEIGEEIEKLEMVIVKRIKEQVELNEDEFVDKIDDFQSVSQDILEIKNRVKQIINIIEDEKYKSFKQDYESFETETKKFIDFLKILESYELKINNSRDKMNQYKRKLFDIRKTIEFGEMMKVVEHKKLKSRNKWISITILFGFVLFILYKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P434</accession>
    <name>A0A1E3P434_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">37S ribosomal protein PET123, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_62678</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039320.1">
        <property type="nucleotide sequence ID" value="XM_019183417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P434"/>
    <dbReference type="GeneID" id="30200663"/>
    <dbReference type="OrthoDB" id="5223508at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23703">
        <property type="entry name" value="mS26_PET12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058940">
        <property type="entry name" value="mS26_fungi"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26163">
        <property type="entry name" value="mS26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="103"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="290" mass="33138" checksum="17AAD3B83CAE1F7E" modified="2017-01-18" version="1">MGKGALKFGGKSGVLPVVRQIFKQPIKPVERKIDPNVGYAENVLQPKGTSREQKLPPVYTVNDRIANTVKLPKKELATGELESLNAKQREKIEKSQLRRRYFIESLTKEEQKLETDKIKEERIKALKEAKEKANIVEETPAAKLTLPTIAKYLDGPLMRNRTPEEKDLLNAKRKANRLNREYNTKLNAASNLLKLYYSAENFITTETDLKKAVEDAFKPGNEIIALINPEQKTHNYNDKIGDALFGTMNARPGLPRIIEHMSGEHAEFHKKVQEADRLRKEQQRDEQLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P464</accession>
    <name>A0A1E3P464_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Purine nucleoside phosphorylase</fullName>
            <ecNumber evidence="7">2.4.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Inosine-guanosine phosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_100349</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Cleaves guanosine and inosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a purine D-ribonucleoside + phosphate = a purine nucleobase + alpha-D-ribose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19805"/>
            <dbReference type="ChEBI" id="CHEBI:26386"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PNP/MTAP phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.1" evidence="7"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039295.1">
        <property type="nucleotide sequence ID" value="XM_019180292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P464"/>
    <dbReference type="STRING" id="683960.A0A1E3P464"/>
    <dbReference type="GeneID" id="30197538"/>
    <dbReference type="OrthoDB" id="10261782at2759"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047724">
        <property type="term" value="F:inosine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070635">
        <property type="term" value="F:nicotinamide riboside hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004731">
        <property type="term" value="F:purine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046115">
        <property type="term" value="P:guanosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006148">
        <property type="term" value="P:inosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034355">
        <property type="term" value="P:NAD+ biosynthetic process via the salvage pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019358">
        <property type="term" value="P:nicotinate nucleotide salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09009">
        <property type="entry name" value="PNP-EcPNPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1580:FF:000004">
        <property type="entry name" value="Purine nucleoside phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011270">
        <property type="entry name" value="Pur_Nuc_Pase_Ino/Guo-sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011268">
        <property type="entry name" value="Purine_phosphorylase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01700">
        <property type="entry name" value="PNPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01697">
        <property type="entry name" value="PNPH-PUNA-XAPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006054">
        <property type="entry name" value="PRK08202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904">
        <property type="entry name" value="METHYLTHIOADENOSINE/PURINE NUCLEOSIDE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904:SF9">
        <property type="entry name" value="PURINE NUCLEOSIDE PHOSPHORYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000477">
        <property type="entry name" value="PurNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="9">
        <location>
            <begin position="32"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="90"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>a purine D-ribonucleoside</name>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>a purine D-ribonucleoside</name>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00000755"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005058"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006751"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00058131"/>
        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000477-2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ60088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="299" mass="32559" checksum="D47355C7D73432CF" modified="2017-01-18" version="1">MTTTQEVYDLLDKAKSFVCSKIPESLSKPKSLIICGSGLGGISSLLQDTTEISYSEIPGFSVSTVPGHAGKLVFGFIGSNKVPVVCMVGRLHFYEGYSFNETTFPVRLAKQLGVEIVVVTNAAGGINPNFKAGDLMIIYDHINIPGFAGWHPLRGPNLDKFGPRFQPLSDAYDLQLRQLFFQQKKKLGVERNIHEGTYFYASGPTFESRAESRVIRTLGGDAVGMSTVPEVIVARHCGLRVLALSLITNECVVDPPASAFDENPKPLDEGMASHAEVLEYANQASLDVQKIIEATVNEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4A1</accession>
    <name>A0A1E3P4A1_WICAA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">WICANDRAFT_78358</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ59722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="GeneID" id="30201957"/>
    <dbReference type="OrthoDB" id="10513439at2759"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
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        <location>
            <begin position="19"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="216" mass="24551" checksum="8AFF98E19679AA1E" modified="2017-01-18" version="1">MNQQSQFNRVNGFGGHTNGLLQVNSNKLQKGTGRSQVTRQTQPLSVSQLHNIQQHQQQQQFQGQEGSFGQGRSVKVQPITTEAQFARAKISSKKLFDFEDDLEFYPNPIETTRIRSQPQQANQQSAINSRMVMNGSPISPYNTPTKPVQFNQQQVHRMGYMSPAQVIGSPQQQQPQRVINHNQIFNSPQNMSNARRLDLSNISPMYQNSSSTTRHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4B5</accession>
    <name>A0A1E3P4B5_WICAA</name>
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        <recommendedName>
            <fullName evidence="12">Serine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.11</ecNumber>
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        <alternativeName>
            <fullName evidence="9">Seryl-tRNA synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Seryl-tRNA(Ser) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_100415</name>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction: serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="3"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019180296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4B5"/>
    <dbReference type="STRING" id="683960.A0A1E3P4B5"/>
    <dbReference type="GeneID" id="30197542"/>
    <dbReference type="OrthoDB" id="10264585at2759"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.40:FF:000003">
        <property type="entry name" value="Serine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000026">
        <property type="entry name" value="Seryl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="16">
        <location>
            <begin position="153"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="279"/>
            <end position="281"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="295"/>
            <end position="298"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="366"/>
            <end position="369"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="13">
        <location>
            <position position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ60183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="458" mass="52649" checksum="D4D0017181E299F5" modified="2017-01-18" version="1">MLDITQFIEEKGGNPEAIRKSQKARYASVELVDEIIADYKEWTKIRFDLDALNKEQNKIQKEIGLKFKAKEDASELVKKKDEIVEQKKQLTQKEQDADKQLKFKVNQIGNIVHESVVISNDEDNNELVRTWKPEGSAEIGKVATATGKEAKLSHHEVLLRLDGYDPERGVRIVGHRGYFLRNYGVFLNQALINYGLSFLAKNEYTPLQAPVMMNKDVMAKTAQLSQFDEELYKVIDGEDEKYLIATSEQPISAYHAGEWFESPAEQLPVRYAGYSSCFRREAGSHGKDAWGIFRVHAFEKIEQFVLTEPEKSWTEFDRMIGLSEEFYKSLGLPYRVVGIVSGELNNAAAKKYDLEAWFPFQQEYKELVSCSNCTDYQSRNLEIRSGIKKMNDREKKYVHCLNSTLCATERALCCILENYQTEDGLVIPEVLRKYIPGEPEFIPFLKELPKNSTSQKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4B6</accession>
    <name>A0A1E3P4B6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_84219</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039546.1">
        <property type="nucleotide sequence ID" value="XM_019185670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4B6"/>
    <dbReference type="GeneID" id="30202916"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="53" mass="6294" checksum="8568DBC9BCB4C420" modified="2017-01-18" version="1">MLLHLHTFLLDNLDKTNRYQQFNKKLVGIHGVQLYPGLMVTVHIHLRNQDEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4L0</accession>
    <name>A0A1E3P4L0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_30697</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PheA/TfdB FAD monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039351.1">
        <property type="nucleotide sequence ID" value="XM_019181754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4L0"/>
    <dbReference type="STRING" id="683960.A0A1E3P4L0"/>
    <dbReference type="GeneID" id="30199000"/>
    <dbReference type="OrthoDB" id="1716816at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012941">
        <property type="entry name" value="Phe_hydrox_C_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038220">
        <property type="entry name" value="PHOX_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050641">
        <property type="entry name" value="RIFMO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43004:SF4">
        <property type="entry name" value="FAD-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43004">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07976">
        <property type="entry name" value="Phe_hydrox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="5">
        <location>
            <begin position="18"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Phenol hydroxylase-like C-terminal dimerisation" evidence="6">
        <location>
            <begin position="420"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="606" mass="67952" checksum="A3EE2511C2BA9A70" modified="2017-01-18" version="1">MPSVNADSQAKPSTKDNVDVLVVGAGPAGLMQAVVLARCGVNVHVYDERTESTANGRADGLQPKTSETLTQLGLFRTIEAQAVKVYDITYWKPGPNSTLVRTGRASHYPSDIVDVRNPYILLVNQGVIEKTLIDDLATRDVDVKRSQKFITYTIDQGNEYPVQSEFKDITTGEHYVVGSKYIVGGDGARSAVRNAMPNSEFNADVTTLRWAVIDGVLKSDFPDFWNKTIIKSKSNGSILGIPRERGMTRLYIELPPEFQYEDLESVQPIIKDMAKRIVSPFTLEWESVEWFTIYRVRQGVATSFVDRGRAFIAGDASHTHSANAAQGMNVSMHDSWNLAWKLALAVKGFGSPDSLFTYQSERQKVGQDLIDFDKIHTSAYKVGDSVALSKNFEQNVKFIAGVGASYSYNVFNKGTRNEILEPGVLPPPVNVLRYVDSNPVQLETVTPVFGQFHICFGAKNFAKSQEFLQNFSAIVEQNQTKFSKIYHQTNVNESKTKLDSLNHKRYTPVSSLFTISFVTAPEKHNNEFELEELPNFFQPYRWSVYLDLQNSRSAFQTWFKNKNESEVGIIILRPDGYVGCSNSFKVTDSPQGVVDWLSDYFEVLDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P536</accession>
    <name>A0A1E3P536_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_15027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039778.1">
        <property type="nucleotide sequence ID" value="XM_019180625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P536"/>
    <dbReference type="GeneID" id="30197871"/>
    <dbReference type="OrthoDB" id="5398685at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="19"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="56" mass="6396" checksum="D11642DEEF979CDE" modified="2017-01-18" version="1" fragment="single">MTQEKAMSDAEFADTYKKLNEGEKTATKIEQMLDDIESKMDELIKEAENINADPQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P572</accession>
    <name>A0A1E3P572_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_27670</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039823.1">
        <property type="nucleotide sequence ID" value="XM_019181397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P572"/>
    <dbReference type="STRING" id="683960.A0A1E3P572"/>
    <dbReference type="GeneID" id="30198643"/>
    <dbReference type="OrthoDB" id="2436667at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13156">
        <property type="entry name" value="KOW_RPL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000014">
        <property type="entry name" value="60S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000915">
        <property type="entry name" value="60S_ribosomal_eL6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049633">
        <property type="entry name" value="Ribosomal_eL6_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041997">
        <property type="entry name" value="Ribosomal_eL6_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01159">
        <property type="entry name" value="Ribosomal_L6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01170">
        <property type="entry name" value="RIBOSOMAL_L6E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="176" mass="19828" checksum="5F36BE7B5CD8B23C" modified="2017-01-18" version="1">MSAVQAPKWYQAEDAPVPKNTRKTARPQKLRASLVPGTVLILLAGRFRGKRVVYLKNLKDNTLLVSGPFKVNGVPLRRVNARYVIATSTKVDVSSVDVSKFDVEYFAREKSSKGKSESKFFGEEQPKKEIKEERVSDQKSVDKQLLAEIKKTPLLKEYLSATFSLKNGDKPHAMKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5A3</accession>
    <name>A0A1E3P5A3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF202 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_84043</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039289.1">
        <property type="nucleotide sequence ID" value="XM_019185638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5A3"/>
    <dbReference type="STRING" id="683960.A0A1E3P5A3"/>
    <dbReference type="GeneID" id="30202884"/>
    <dbReference type="OrthoDB" id="2243669at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033254">
        <property type="term" value="C:vacuolar transporter chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016237">
        <property type="term" value="P:microautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF1">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="DUF202" evidence="6">
        <location>
            <begin position="24"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="122" mass="13538" checksum="628C8A5327834473" modified="2017-01-18" version="1">MSSNPLLQTTPGKRIALPTRVEPKVFFANERTFLSWLNFTVILGGLGVGLLNFGDKVGRVSAGLFTLVAMATMLYALVTYHWRAVAIRKRGSGPYDDRFGPTMLCFFLLVAVVVNFILRIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5K2</accession>
    <name>A0A1E3P5K2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zinc transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_89825</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Functions as a zinc transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039860.1">
        <property type="nucleotide sequence ID" value="XM_019186197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5K2"/>
    <dbReference type="STRING" id="683960.A0A1E3P5K2"/>
    <dbReference type="GeneID" id="30203443"/>
    <dbReference type="OrthoDB" id="78669at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904257">
        <property type="term" value="P:zinc ion import into Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000014">
        <property type="entry name" value="Cation efflux protein/ zinc transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045316">
        <property type="entry name" value="Msc2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45755:SF4">
        <property type="entry name" value="ZINC TRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="285"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="463"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="515"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="547"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="10">
        <location>
            <begin position="363"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU369017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="582" mass="64057" checksum="C96C158D654B3736" modified="2017-01-18" version="1" fragment="single">MAQEHQHAHSHSQDQSHSHSHEEKDNHHSHDHSHHRDEQPVECEQNSTLDKYIDIAHIPLLLAYPTSLLASSLIFQEGQLNTITDHNVSSINEFLLSILYIPVFLASILTIIYTYMGNGATATTPITEKSIEQNLKSNKVFLGLLGLVVANNFLGSVRTVVLSLVMIINVSFPYVLLSLAFDLYCLYLKGKLELLPSTLGTSQFYFIIFAYFILFQIKDSLRDFNQLQHKNDVTIPGVLLLLSGLGIIWNYTAVQLSMILVTLGCGIILNVTKLDLAVIHFKQGAPTVTGIISILLCFLSYNQDYISNKLLFAGEVLLILITGLIPVDHSLFKSSSHSTKSTVNEQQTILTQLLSHKDTRAIFSFLLLNASFMFVQFLYSFRSRSLGLLSDSLHMALDCTSLALGLVAGVLAKRPPSEIYPFGLARIETLAGFANGVLLIGIVAGIFIEGIERIINPVSLEKTGELLIVSFLGLVVNIVGIFAFNHGHEHGGHSHGHSHSHGDGDVHDNENMKGIFLHILADTLGSAGVVVSTLLTSWFHSNIFDPIASLFIAVMIFVSAIPLIRSSASNLLLSLDDKKDEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5W6</accession>
    <name>A0A1E3P5W6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">CSC1/OSCA1-like 7TM region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_27864</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039877.1">
        <property type="nucleotide sequence ID" value="XM_019181420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5W6"/>
    <dbReference type="STRING" id="683960.A0A1E3P5W6"/>
    <dbReference type="GeneID" id="30198666"/>
    <dbReference type="OrthoDB" id="1689567at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF5">
        <property type="entry name" value="RE44586P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="421"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="470"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="515"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="558"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="606"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="633"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="670"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="696"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="10">
        <location>
            <begin position="22"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="11">
        <location>
            <begin position="201"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="9">
        <location>
            <begin position="420"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="274"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ60670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="792" mass="90404" checksum="616BB7859D1811F8" modified="2017-01-18" version="1">MDILKSSDNIDLRKPSARVAKTQLLIAFILGLFAFLTFCILRNTWSKLYISRLTRRKGLPTIPTKSLFGWIPVIYNINEQDVLNHAGLDAFVFLGFFKMAIKLLSLCAFISIAIISPVRYHYTGRYDQGDGDNDGNNDGDGVEKKIGNEPDIPGGYKPYLWMYVGFTYVFTLLTFYMLIKQTKHVVQTRQQYLGNQNSITDRTIRLSGIPPELRDEESLKKHIEELNIGKVSSITICREWGQLNRLFKHRKQILLKLEDYWADTVQRPYHDAPSDDETENEEQAGDTTVESIIDEGSADSIRDYSTRNAAVTLGAQPYEHKRPKLKTGFLGLFGKEVDAIDHLTNQLDVLDEEILRARQRHFPATPTAFITMDTVASAQMAAQAVLDPHVHYLITRLAPAPHDIIWDNIVLSRKERIVKNYTITIIIGIISVSLIVPVGYLATLLNPKTIKKFWPGLGELLQNNEWAKNIVSGLLPTYIYTIMNFVIPFIYVWLSSKQGYISHGDEELSAVSKNFFYIFVNMFLVFTTAGTASNYWGFLSDTTKIAYQLAQSLNELSLFYVDLIILQGLGMFPFKLLLFGSILRFPFFKAGCKTPRDYRELYKPPIFNFALHLPQPLMILIITIIYSVFSTKILVSGLIYFIIGFYVYKYQLIYSVVHPPHSTGKVWPIVFRRIVVGLLLFQLTMAGTLAAFQDGYLLATVLTPLPLATISFLWNFQKNYLPLSFFIALRAIEREPESSQTLDETREQNQTYEYPFLVQPLDGPWISIEGDHAVVANADGTKRRRVNLLEWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P622</accession>
    <name>A0A1E3P622_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Luciferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_61452</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NtaA/SnaA/DszA monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040096.1">
        <property type="nucleotide sequence ID" value="XM_019183178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P622"/>
    <dbReference type="STRING" id="683960.A0A1E3P622"/>
    <dbReference type="GeneID" id="30200424"/>
    <dbReference type="OrthoDB" id="5561043at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051260">
        <property type="entry name" value="Diverse_substr_monoxygenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016215">
        <property type="entry name" value="NTA_MOA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03860">
        <property type="entry name" value="FMN_nitrolo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011">
        <property type="entry name" value="ALKANESULFONATE MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011:SF30">
        <property type="entry name" value="XENOBIOTIC COMPOUND MONOOXYGENASE, DSZA FAMILY (AFU_ORTHOLOGUE AFUA_6G01920)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000337">
        <property type="entry name" value="NTA_MOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="2">
        <location>
            <begin position="47"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00033748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="473" mass="53290" checksum="75FDD9F8B8B3B820" modified="2017-01-18" version="1">MSAEPQLKKQKPNGKKKLHFNAFEIFSPGHSNPGQWSNPKDLSSTKDTIEFWENNAKLLEKGKFTCYFLGDTLGGFEVYEGSRSPAIKIGTEFPVLDPFLIVPSMSRVTEKLGFGVTATTTFEPPFLLARRLATLDHLTKGRIGWNIVTSWNNTSAKALGYDKIMDHDERYQMADEYLELLYKLWESSIADDAVVIDKENQTFTDPSKVQVIKHEGNYFKCETPFIVHPSLQRTPFLFQAGTSKAGVEFASKHAEGIFMASHSPEVLAPKIQQLRNLAKEKGRDPQSIKVFPLFTPIVGKDDEDANLKYEEIKKYRSTEGGLIFFSALTGIDLSKYDLDEEINVEDSKVPQVVQSAVEHLFKEQKDIKWTPRKVGEELSLGGNGPVIVGGPEKVADYIENFVEIADIDGFNITPVVNPGSYEDIIEYLIPELQKRGLYWDEDEDPHGITLRSHILGQNHVEPGHPATNFKYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P662</accession>
    <name>A0A1E3P662_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_51626</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040060.1">
        <property type="nucleotide sequence ID" value="XM_019182674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P662"/>
    <dbReference type="STRING" id="683960.A0A1E3P662"/>
    <dbReference type="GeneID" id="30199920"/>
    <dbReference type="OrthoDB" id="540503at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF28">
        <property type="entry name" value="VERY-LONG-CHAIN ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="10">
        <location>
            <begin position="129"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="272" mass="31565" checksum="D1E6FA240D437F83" modified="2017-01-18" version="1">MTSIKVTSRSKNIKSGEFSTSSYKDFVSQLSKHSRLNSNRLRITNLDKKPINDDSILNESEVYVKDLGPQIGWRTVYLIEYLGPILIHFLVYQLASKPKDYELIYYMNLIHYGKREVENVFVHRFSNSTMPLFNLFKNSGHYWVLGGSLSLMYLGGFGNLPISLNLNKDYLFYIWCGCEFFNAISHIQLRLLGDKTVRKGKAREVPSGGLFELFISPNYTMEIYGWIIVFLLNPNVFTLVFLLVGATQMYFWSIKKQEKYGTKRAFLVPFVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P766</accession>
    <name>A0A1E3P766_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial carrier protein LEU5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_28300</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040473.1">
        <property type="nucleotide sequence ID" value="XM_019181471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P766"/>
    <dbReference type="STRING" id="683960.A0A1E3P766"/>
    <dbReference type="GeneID" id="30198717"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015228">
        <property type="term" value="F:coenzyme A transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000151">
        <property type="entry name" value="LEU5p Mitochondrial carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="354"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="36"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="135"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="296"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ61266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="43434" checksum="DC62EA6689A09E9B" modified="2017-01-18" version="1">MNQETKLSPIINNTKVHTDDSTPFEAKASLDKSSAEYIIKTGLAGGLAGSSGKTLIAPLDRIKILFQTSNPLYKQYSGSIFGMFKAGKKIYINDGIFGFFQGHSATLLRIFPYAAIKFVMYEQVRRILIPNDDYETSVRRLMSGSIAGLCSVFVTYPLDLIRVRLAFDTKHHSSTTIPIHHKNHIHNIRKGLLVSTIQQIYKEHPIVDKQFFLINMLKKNLPNKISNLTNFYRGFIPTLMGMVPYAGVSFWTHDFVHDVLRLPLIAPYTVDQTHPGHFQLMSNGSKPSTHEQRSPLNTWAQLLAGGIAGMFSQTAAYPFEVIRRRMQVGGVNNGNFIGIGSISKKIWKERGFKGFYVGLSIGYIKVIPMVACSFFVYERTKYHLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P768</accession>
    <name>A0A1E3P768_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_61774</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040420.1">
        <property type="nucleotide sequence ID" value="XM_019183246.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P768"/>
    <dbReference type="STRING" id="683960.A0A1E3P768"/>
    <dbReference type="GeneID" id="30200492"/>
    <dbReference type="OrthoDB" id="276721at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051207">
        <property type="entry name" value="ComplexI_NDUFA9_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126:SF16">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="262" mass="29317" checksum="7073931ADA84904D" modified="2017-01-18" version="1">MSKLVVLGGNGFLGRRICQAGIESGFRVTSLSRSGKPPRLTPKEDSSWIQKVNWRPADIFKPESYKEELKDATSVVHSIGILLENQNYKKTINSNSSILSEFSNFLKPSNPLTKSSFNSYESVNRDSAILLAETFQETTNSSDPAFVYISADKGFPGLPKGYINSKREAEQEIQVLEKLRPILVRPGFMYDEVSNQDNVRSSIKTFVDIANWGNSTILGNRVGLFNELIRPTVSTQRVAEAIIYRIQDKTFKGVVTLDDLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7D8</accession>
    <name>A0A1E3P7D8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_99841</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Protein modification.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040546.1">
        <property type="nucleotide sequence ID" value="XM_019186701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7D8"/>
    <dbReference type="STRING" id="683960.A0A1E3P7D8"/>
    <dbReference type="GeneID" id="30203947"/>
    <dbReference type="OrthoDB" id="19692at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010828">
        <property type="term" value="P:positive regulation of D-glucose transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23811">
        <property type="entry name" value="UBCc_ScCDC34-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000063">
        <property type="entry name" value="CDC34p Ubiquitin-conjugating enzyme (E2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="9">
        <location>
            <begin position="5"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="187"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="201"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ61339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="238" mass="27050" checksum="D5604A25806DA99D" modified="2017-01-18" version="1">MSSTSAASLLLRQFRELTDPKKAIPSFHIELEDDNIFLWNIGVMVLNEESVYHGGYFRAQMKFPSDYPFAPPTFRFTPAIYHPNVYRDGRLCISILHQGGDPTSDEPDAETWSPAQTVETVLISIVSLLEDPNPSSPANVDAAVDWRKRPDVYKKKVELEVERSKQDIPQGFVMPTSASAYLATHSAPVEDEVVDENFWYDSEEEESYDDDSIMDDVEGNEEEDEEEDEELSEEEGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7E9</accession>
    <name>A0A1E3P7E9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_90531</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycetes</taxon>
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            <taxon>Wickerhamomyces</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3P7E9"/>
    <dbReference type="GeneID" id="30203485"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="188" mass="20992" checksum="8A51048401AB3C48" modified="2017-01-18" version="1">MPQIEDQYIWDILQANTLNSSWTYGTSFQDQKTNNNPPEKEERKPINNTLGGGADEDDFEELASHSDDFEEEHQRGMDSLSIDEENTKSPGRNSPKKESDTDTGSSSENSKNTSKQASASNTPSPTKQENPSKEASSPSKSPSSSKESMKKETQTKPKRHFGAMSRKRKVHHSGFSPIKAQKQFHSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7H5</accession>
    <name>A0A1E3P7H5_WICAA</name>
    <protein>
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            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
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        <name type="synonym">Hansenula anomala</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycetes</taxon>
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            <taxon>Wickerhamomyces</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ61366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7H5"/>
    <dbReference type="GeneID" id="30203506"/>
    <dbReference type="OrthoDB" id="10537047at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="164" mass="18561" checksum="AC9B6C7EE476AD1C" modified="2017-01-18" version="1" fragment="single">MLKNIKTSSTLRAVSLSPKRAFSQYKPTRNEHHDHDHHHGETLNEEEKLSSSYIYGFIATGAVVAYWAINQSYAKSHNGESLTSKILTPVKQKEELIEDMKVYNEKLKKSSDLRDAMSIPAPNKLFHEFTSMQVIPSGSPSNYVPGSQLDLDNIGPRRQKKSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P862</accession>
    <name>A0A1E3P862_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxidation resistance protein 1</fullName>
        </recommendedName>
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        <name evidence="6" type="ORF">WICANDRAFT_77779</name>
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    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040339.1">
        <property type="nucleotide sequence ID" value="XM_019184610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P862"/>
    <dbReference type="STRING" id="683960.A0A1E3P862"/>
    <dbReference type="GeneID" id="30201856"/>
    <dbReference type="OrthoDB" id="26679at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032984">
        <property type="term" value="P:protein-containing complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF62">
        <property type="entry name" value="MUSTARD, ISOFORM V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="5">
        <location>
            <begin position="24"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="206" mass="23410" checksum="E377232EAD6058E0" modified="2017-01-18" version="1">MLNLDTPPLTPLELTGYSSSTRNRLLTPELGEEIRNLLPARLQIESKWKLLYSLEQHGASLKTLYNNTKNGSTSSNTGYDKSGYLLVIKDDLHDVFGGYVNEYFHPTDSKRYSGNGECFLWKSRKVKDDEGTHIQFQAFPYTGENDYLIYGTSEFLSIGGGEGRYGLWIDQNLLKGNSHHTKTFGNEQLSRKGEKFEVLGVEVWRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8E1</accession>
    <name>A0A1E3P8E1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_78110</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019184663.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3P8E1"/>
    <dbReference type="GeneID" id="30201909"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071232">
        <property type="term" value="P:cellular response to histidine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042327">
        <property type="term" value="P:positive regulation of phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071264">
        <property type="term" value="P:positive regulation of translational initiation in response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006448">
        <property type="term" value="P:regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000104">
        <property type="entry name" value="ATP-binding cassette sub-family F member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000882">
        <property type="entry name" value="Translation initiation regulator (Gcn20)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF117">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="199"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="532"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="283"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="752" mass="84734" checksum="C2C08339C535B35C" modified="2017-01-18" version="1">MSNIGLQIRQAAPSIDPIVADYAVGYINHVQHSTTDSVLSQQLNIEQEISFISDLLRNAGGDDTKISKLADTLTSKLGEELKQNQSKLELTGDTSKRLLDINLLKNHASKRNINTSLSLLGVTRDIEHTGRHVESKVDKKKLEKAERKIAAKVAKRNNQFVQYEASKLINDSQEENYDEFFLKINPIEFGSGAGKSKDIKIDTFDLYVGDGQRILSDAQLTLSYGHRYGLVGQNGIGKSTLLRALSRRELNVPKHITILHVEQEIRGSEITALQSVLDADVWRKQLLTEEKQHNDRIAEIENLRSQFEEESLEVKKLDNERDDLESHLQEIAEKLYEMESDKAESRAASILFGLGFTKESQQRPTNSFSGGWRMRLSLARALFCKPDLLLLDEPSNMLDVPSITYLANYLQTYESTVLVVSHDRAFLNEVATDIIHQHSERLDYYRGADFDSFYSTREERRKNDLREYENQMAYRQHLQTFIDKFRYNAAKSSEAQSRIKKLEKLPVLEPPEQDKAITFKFPDPEGLSPPIVQLKDVSFGYSSNDLLLNHVDLDIQMDSRVAIVGANGCGKTTLLKILMETLSPLDGFVSKNPRLRIGYFAQHHVDSMDLTTSAVDWMSKTYPGKTDEEYRRHLGSFGITGTLGLQKMQLLSGGQKSRVAFAALCLNNPHILIMDEPSNHLDTAGLDALSDALKNFKGGVLMVSHDVSVINTVCNEIWVSEDGTVKKFQGTIYDYKKYILAAADASGVVKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8E9</accession>
    <name>A0A1E3P8E9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_78118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040701.1">
        <property type="nucleotide sequence ID" value="XM_019184666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8E9"/>
    <dbReference type="GeneID" id="30201912"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="611" mass="69974" checksum="1F47BAEF20B2DE30" modified="2017-01-18" version="1">MLFSLTHKHFFKSSPKKLARLASSVRWKSTKLANLLNNSKEPPMLHYNQYLKKVDEPGDYAIGERYADFMDQLMLDENRKQLAFAKIALSTPSLLTGEETSSTTKPRYNSEVRDTKHEPVSGCSISSSKFKNTNSKTRVERIDYSSDPKNLEDTNKNYQNLKKHQNRTPSMERRMLSLLDIESRILLQSPQSEADVQYSFCAKVVQPICSMIEMVYGVTIQFNQQVYNKECKIFPGITIKSPNAKGSVVVEVKKHLPAANEFNKQKISELLKINGVQQVINSISSSGSRFGILTSFKTTLIVWINHDNVTFTDEECILDIKYLVIDHLEPYCTLKSIIHNLLIDQFTSPLDVQHERYEKSMKFKANFSTSPAIVEKKLELSRQYMKLQYDAKTLGGNEENIEVVIDSNVTPIGTLKDYDHMVRSIYLKKTHFNPDDKSEDSVFIKIWDPVTFGMNDDPTHNQEQMEFEYLNSVHNQTIVNKCIAGSPKYWKRGFLVVKDENLKILSLGKFMAFEVSNMSSQMKYQPMNVILPKALSQLEVLNTKGLGLYVNIEAEDDRFNPEKDMILQKNGELSIINVAGTGEHPRSDSGRKFDRRVLIDGMKMFYEGFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8V0</accession>
    <name>A0A1E3P8V0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_24870</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040851.1">
        <property type="nucleotide sequence ID" value="XM_019181067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8V0"/>
    <dbReference type="STRING" id="683960.A0A1E3P8V0"/>
    <dbReference type="GeneID" id="30198313"/>
    <dbReference type="OrthoDB" id="4078873at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010290">
        <property type="entry name" value="TM_effector"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF58">
        <property type="entry name" value="LOW AFFINITY HEME TRANSPORTER STR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05977">
        <property type="entry name" value="MFS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="129"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="390"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="476"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="551"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="64"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ61644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="669" mass="74440" checksum="E63EDA4F12CD58D1" modified="2017-01-18" version="1">MSVSDRKLDNSSLDQEVTNSHHVPVIDEEKITEPEYVSRGVRRMEKLKKVMSETKKGKHMRLLLCACIIITEFVVALDGSTTSSYQPYATSAFKHHSMLSTLSIATSIMSSVSQPIIAKIADVTSRPTCYILVLSFYTMGYIICAASSTISAYVIGSVFVTIGRSNIDMINTIILADIIPLKYRGFTFGLTASWYLITVWISGGIASHFITVNWRWGYGMFSILMPATIIPCIACMGYLEHYAQTVFPDNDHFDNENKTKKTPKDFAKLFWNYVVESDLFGLIMMAFGWSLLLLPFSLYSSAQGGYKNPSLIAMFVVGALLLIIYACYEVWIAPFPSVPKRVLTNRTFITAVTVDFFYLCGAMLLQLYLSSYVGVVKDWSYRNWSYFNNTQTLSICFFGVIVGLIQRVTHRTKFLQVGGLIIQVVAMIIALWARYGNASTGALVWVQILLGMGGAASNIGSQVASQASVPHQDVALAIALLLQWSTIGSAIGAAIGGALWENKLMKALRTYLPSSVSDDDVAEMYSDFSAIQKYPMDSDVRQGAILAYNHVVYVLFALALAFTALAFFASLFQKNYYLGDDQNAVEYANMVNRPTNRFARILDWIDNPFAYYKNKKAEKQAQDHTTGQSTGVIEETSEFNDDTNLQNRQVKEDSVVNTKAGVSEMVSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P965</accession>
    <name>A0A1E3P965_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_60027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041160.1">
        <property type="nucleotide sequence ID" value="XM_019182924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P965"/>
    <dbReference type="GeneID" id="30200170"/>
    <dbReference type="OrthoDB" id="2141050at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39218">
        <property type="entry name" value="OXIDOREDUCTASE 14 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12110)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39218:SF1">
        <property type="entry name" value="OXIDOREDUCTASE 14 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12110)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="86" mass="9736" checksum="2DC5CA9420CA0068" modified="2017-01-18" version="1">MVGTLSYVGWAGFGVLARGFQLGILRRPWGHFKMGYVYSAALWTGIGYFFYKVVDAEDEILNERVKKLHEARARRKQEAAVPAAEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P988</accession>
    <name>A0A1E3P988_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Guanine deaminase</fullName>
            <shortName evidence="8">Guanase</shortName>
            <ecNumber evidence="8">3.5.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Guanine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_60049</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>guanine + H2O + H(+) = xanthine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:14665"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Purine metabolism; guanine degradation; xanthine from guanine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the metallo-dependent hydrolases superfamily. ATZ/TRZ family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.3" evidence="8"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041182.1">
        <property type="nucleotide sequence ID" value="XM_019182930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P988"/>
    <dbReference type="STRING" id="683960.A0A1E3P988"/>
    <dbReference type="GeneID" id="30200176"/>
    <dbReference type="OrthoDB" id="194468at2759"/>
    <dbReference type="UniPathway" id="UPA00603">
        <property type="reaction ID" value="UER00660"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008892">
        <property type="term" value="F:guanine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006147">
        <property type="term" value="P:guanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000022">
        <property type="entry name" value="Guanine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014311">
        <property type="entry name" value="Guanine_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051607">
        <property type="entry name" value="Metallo-dep_hydrolases"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02967">
        <property type="entry name" value="guan_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11271">
        <property type="entry name" value="GUANINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11271:SF6">
        <property type="entry name" value="GUANINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="9">
        <location>
            <begin position="82"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ61975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="469" mass="52299" checksum="A60A73A24E652B41" modified="2017-01-18" version="1">MTVPIFNKQFTPAKATLYYGNFIHTPKLGAIEVFTNSGALVDKDGFISKFYADKTLNEILKLIDDTTADVVDISANAKSFFFPGFIDTHIHAPQYPNNGIFGYSTLLDWLTTYTYPLEASLKNLDKAELVYNKVITRTVANGTTCAAYYATIDTDATNLLTDLALHHGQRAFIGKVCMNDNAPDYYIETLEESKESTMEVIDHIKQVDPKNELVAPILTPRFAPSCTSELLNWLGDLKKEHHLHSQTHISENKGEIEWVAELFPNSKSYADVYNDHGLLDDKTILAHAIHLSDHEKDLIRSKGSGISHCPISNSAITSGEARIRWLLDNDIPVGLGTDVSGGFSPSVLSTARQALLVSRHLAMKTSDHNKLSVEDVLYLATVGGAKVVGLDDKVGLFKEGMKWDAQLIDLKAKDSQVDVFEWQVPEETESTSKDDFVKFQNMVAKWLFNGDDRNTTKVWVNGRQVHERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P999</accession>
    <name>A0A1E3P999_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_25680</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041001.1">
        <property type="nucleotide sequence ID" value="XM_019181172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P999"/>
    <dbReference type="STRING" id="683960.A0A1E3P999"/>
    <dbReference type="GeneID" id="30198418"/>
    <dbReference type="OrthoDB" id="1732682at2759"/>
    <dbReference type="UniPathway" id="UPA00528">
        <property type="reaction ID" value="UER00586"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042853">
        <property type="term" value="P:L-alanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000012">
        <property type="entry name" value="alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000151">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045088">
        <property type="entry name" value="ALAT1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751">
        <property type="entry name" value="ALANINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751:SF29">
        <property type="entry name" value="ALANINE TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="114"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="507" mass="56934" checksum="78E8CE87AD3C4B6B" modified="2017-01-18" version="1">MLHFQTTPSRALLVTRFIQHSKRHNSFFPAEKLTKKDLNPRVLGAEYAVRGLIPNKAEELREKLRHNPDSLPFKEIINANIGNPQQLRQRPLTFYRSVLSILQNPKLLETYHDYPKDVVHRARVLLSKIGSLGAYSHSQGVQYIREQIAHFITKRDGGAISLPENIYLTGGASDGVKAVFEVILDGERTSGVMIPIPQYPLYTAAITLNNATPISYYLNEHDNWSTNPHEIEELVQTSNKFGIKPKILVVINPGNPTGSVLSKTAIEDILTIAAKRGLVIIADEVYQENIFEGKFHSFKSVLSDLQHKHAHLYDNVQLVSLHSTSKGVTGECGQRGGYMELVGFKPEIHEMFLKLASISLCSVVSGQALVEMMVNPPKKGDESYELDQKERTAIHETYKSKANALYEAFSKLENIEVQKPQGAMYLFPKIILSESIIAHAKDLGLKPDEFYCLELLGNTGICVVPGSGFGQVEGTFHVRTTFLPPGQEWIDKWAKFHKEFFLKFRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9G7</accession>
    <name>A0A1E3P9G7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 subunit G</fullName>
            <shortName evidence="5">eIF3g</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 RNA-binding subunit</fullName>
            <shortName evidence="5">eIF-3 RNA-binding subunit</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Translation initiation factor eIF3 p33 subunit homolog</fullName>
            <shortName evidence="5">eIF3 p33 homolog</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TIF35</name>
        <name evidence="9" type="ORF">WICANDRAFT_76234</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eIF-3 subunit G family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041262.1">
        <property type="nucleotide sequence ID" value="XM_019184347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9G7"/>
    <dbReference type="STRING" id="683960.A0A1E3P9G7"/>
    <dbReference type="GeneID" id="30201593"/>
    <dbReference type="OrthoDB" id="639027at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006415">
        <property type="term" value="P:translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12933">
        <property type="entry name" value="eIF3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12408">
        <property type="entry name" value="RRM_eIF3G_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03006">
        <property type="entry name" value="eIF3g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017334">
        <property type="entry name" value="eIF3_g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024675">
        <property type="entry name" value="eIF3g_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034240">
        <property type="entry name" value="eIF3G_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12353">
        <property type="entry name" value="eIF3g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037949">
        <property type="entry name" value="Transl_init_eIF-3_RNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="200"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="152"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="281" mass="30993" checksum="7A9DAEAE15ED48A0" modified="2017-01-18" version="1">MADIEQRVGEWADDEPQELQEPVVKTNADGTKTIISYRIKEDGKKVKITQKVKEVIVKESVNKEVAKRKKWAKFGAEKNSAPGPDFRTTQVGEPVYLRLGTAWKALEKEEEEKSATEAAKTSTSTRIKCRTCGGAHYTAKCPFKETLGGDPAISGGASPAPESTAGSPAPSAPGKYVPVHLRNRQPGAEGEKRFDRDDSTTLKVSQLNEVVTEQMIRNELFANFGPLVRVNIVRNKETGRSKGLAFVQFASETTAQRAMEALHGRGYHSLILHVEFTKPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9Q1</accession>
    <name>A0A1E3P9Q1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">B-related factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_25422</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041350.1">
        <property type="nucleotide sequence ID" value="XM_019181142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9Q1"/>
    <dbReference type="STRING" id="683960.A0A1E3P9Q1"/>
    <dbReference type="GeneID" id="30198388"/>
    <dbReference type="OrthoDB" id="511529at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000994">
        <property type="term" value="F:RNA polymerase III core binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000995">
        <property type="term" value="F:RNA polymerase III general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001156">
        <property type="term" value="F:TFIIIC-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001112">
        <property type="term" value="P:DNA-templated transcription open complex formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070898">
        <property type="term" value="P:RNA polymerase III preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd20553">
        <property type="entry name" value="CYCLIN_TFIIIB90_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20554">
        <property type="entry name" value="CYCLIN_TFIIIB90_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000002">
        <property type="entry name" value="Transcription factor IIIB 90 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000007">
        <property type="entry name" value="Transcription factor IIIB 90 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.650">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011665">
        <property type="entry name" value="BRF1_TBP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR IIIB 90 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07741">
        <property type="entry name" value="BRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="14">
        <location>
            <begin position="2"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="454"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ62143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="527" mass="58854" checksum="0AC5A9C129F078E1" modified="2017-01-18" version="1">MLAKKCKGCGCSDFTRDIHSASSDLACQNCGRVFEENPIVSEVTFGESSNGAAVVQGAFVGADQAHATFAGGPRGNSMDSRTKTLANAKRKIRAVATALRIPEYVSDAAYQWFSLALAYNFVKGRKSQNVIAACLYIACRKEKTHHMLIDFSARLQISVYSVGATFLKMVKALHITKLPLADPSLFIQHFAEKLDFGDDRLKVVKDAVRLAQRMADDWIHEGRRPAGIAGACLLLAARMNNHRRTHGEIVAVAHVGEDTLQRRLNEFKQTHAGELTVKEFRESDDYSPSQPPSFKKNRLKEQKLNEKYKYNSEPNNSDDKVGNELLDKLFGSVDLNSQEILDQLERVKQRQRKDIERIKSTNNIGSRVASPVPTHEDRLQFDEDRPRNLDKLPTTSDVLSNVSSDAMNFDDIDDDEIDAFLLTEEESSLKERVWIGLNEDFLLEQEQKRLKAEADEISGHQQARKKKKPQPKSENDALNDVENDGIQGAINSIGEPSSASAAARSVLSKKASKKINYMALNNLFEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9Q3</accession>
    <name>A0A1E3P9Q3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_60165</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041305.1">
        <property type="nucleotide sequence ID" value="XM_019182949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9Q3"/>
    <dbReference type="STRING" id="683960.A0A1E3P9Q3"/>
    <dbReference type="GeneID" id="30200195"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00157">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001179">
        <property type="entry name" value="Rho family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="183"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="205"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="216" mass="24302" checksum="3BF11A21432E6564" modified="2017-01-18" version="1">MRSFKCVVVGDGAVGKTCLLISYTTNTFPQDYIPTVFDNYTKNTLFEGAHYKLELWDTAGQEDYDRLRPLSYPQTDAFLVCFSIVEPSSFKNIKSKWIPEIRHHSTDSVQILLVGTKADLREDPHTLDRLEESGNEPISRADGKKLVKELGLYDYLECSAATQEGVEEVFDSVIKAILETEKETTAPVSMPQKNNASQQATVISKKPRKKSKCTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9R5</accession>
    <name>A0A1E3P9R5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4045 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_87368</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041366.1">
        <property type="nucleotide sequence ID" value="XM_019186104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9R5"/>
    <dbReference type="GeneID" id="30203350"/>
    <dbReference type="OrthoDB" id="3981264at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025118">
        <property type="entry name" value="DUF4045"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13254">
        <property type="entry name" value="DUF4045"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4045" evidence="2">
        <location>
            <begin position="5"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="540"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="610"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="674" mass="76620" checksum="697136C0C18FB21D" modified="2017-01-18" version="1" fragment="single">MDVEENVEDFLERVKQLDLQRQKEDEMRRKQLENEIQSYTNRESVYNISIPKPIAREEEPATKATIPNSESLQNFNKSISTQYRIDIDDLVYESAYNYEKSQNSSPVKNSFNSYNRNDDSSFRASTGSISREKTFKKYDNIPDIVRFDDAMAKSAPNYRTSNFTSSRPSRYSKFQLTDEEKKSIDSTIEGLIGGDHKVTEVQDENEQDERRIPKPLTRDRKGGQFSTPSTPQLQSNNVWSNAAKQKSTLAPPVSLSNQTSHLKRSPRKTELVTSPIRPEISSPIKKSPAKLDFSRYTSEDKHKPPPIAPKPLGFKETKNSWLGSAVNNSSSTPQWQDTTSKIQLNKPEDKKASWLNSAYKHSTSNPQWQDTTPNLQLDNANTSDPKTSWLNSAMQRTSHKEFELPKSKIKPIVPSKSNTLSKMIENDELSKQKQHSTLKELESQKLNHINSPNRPIPQLSHELNYSPTILKKTRTPSPIRQDIQEEPDFIKQRLSPTPNPASLSASHQIDAEALAYKSKLKPPLPQRKPSLDLPEALKKADKLKPALPKRKPSIKIPEALSQKEQLRQPTKMEKVGEPTPEAMSKKTQLKPALPKRKPSLKEVEALEQLQRMKSKTSSKQPPPITRSKPRVFSNSKEILQNQLGSLKSTKNLQKDEASLNLDRILKKATTDTDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA11</accession>
    <name>A0A1E3PA11_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_60323</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041462.1">
        <property type="nucleotide sequence ID" value="XM_019182970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA11"/>
    <dbReference type="GeneID" id="30200216"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="235" mass="26821" checksum="F18EC693BCC59145" modified="2017-01-18" version="1">MNQNTSHQSSHQLQAQTTSININNNQQQQVQTTNHLQDNTTNHNNHDHHHCSPNTTEAATETNSTDDSTKSSSNSNYPFGFIKSSKDILAGVLNTLVGLNVSCFEKLIEPFLLGTFPKSPTQITLLKFNHDDYFTEHSTADDSQSPGNFTFNKVGVFFPFHYWFQRRKVVFADSLEVVFARRFTLGFYYLIWIIPMTYYWELFPPSAWLVAVFHVFTLNNDALSGEADTKSSLHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA25</accession>
    <name>A0A1E3PA25_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein eS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RPS1</name>
        <name evidence="8" type="ORF">WICANDRAFT_25693</name>
        <name evidence="9" type="ORF">WICANDRAFT_88474</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
            <strain evidence="8">NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (25S, 5.8S and 5S).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the eukaryotic ribosomal protein eS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040944.1">
        <property type="nucleotide sequence ID" value="XM_019181175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA25"/>
    <dbReference type="STRING" id="683960.A0A1E3PA25"/>
    <dbReference type="GeneID" id="30198421"/>
    <dbReference type="OrthoDB" id="9834376at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03122">
        <property type="entry name" value="Ribosomal_eS1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001593">
        <property type="entry name" value="Ribosomal_eS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018281">
        <property type="entry name" value="Ribosomal_eS1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027500">
        <property type="entry name" value="Ribosomal_eS1_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11830">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01015">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01397">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01191">
        <property type="entry name" value="RIBOSOMAL_S3AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="initiator methionine" description="Removed" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="modified residue" description="N-acetylalanine; partial" evidence="6">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="256" mass="28769" checksum="C78C72C6AD2E830A" modified="2017-01-18" version="1">MAVGKNKRLSKGKKGLKKRVVDPFTRKEWFDIKAPSTFENRNVGKTLVNKSTGLKNAADALKGRVVEVSLADLQGSEDYSFRNVKLRVDEVQGKNLLTNFHGMDFTSDKLRSLVRKWQTLIEANVTVKTSDDYVIRVFGIAFTKRQANQIKKTTYAQSSHIRAIRKEMVNIITKEVQNSTLAQLTSKLIPEVIGREMEKATKGIFPLQNVHIRKVKLLKQPKFDLGSLLALHGEASSEEKGKKVAGGFKDEILETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA42</accession>
    <name>A0A1E3PA42_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Anamorsin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_22990</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the anamorsin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041492.1">
        <property type="nucleotide sequence ID" value="XM_019180972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA42"/>
    <dbReference type="STRING" id="683960.A0A1E3PA42"/>
    <dbReference type="GeneID" id="30198218"/>
    <dbReference type="OrthoDB" id="311633at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007785">
        <property type="entry name" value="Anamorsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046408">
        <property type="entry name" value="CIAPIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13273">
        <property type="entry name" value="ANAMORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13273:SF14">
        <property type="entry name" value="ANAMORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05093">
        <property type="entry name" value="CIAPIN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anamorsin C-terminal" evidence="10">
        <location>
            <begin position="18"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="75" mass="7910" checksum="A8710BD80BD6C4FE" modified="2017-01-18" version="1" fragment="single">PNSNGGKKRRKACKDCTFQFSAKELTEIDFTVEGKTGGCGSCSLGDAFRCSGCPFLGLPAFKPGQAITLDSIDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA43</accession>
    <name>A0A1E3PA43_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Coatomer subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_87542</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041445.1">
        <property type="nucleotide sequence ID" value="XM_019186116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA43"/>
    <dbReference type="STRING" id="683960.A0A1E3PA43"/>
    <dbReference type="GeneID" id="30203362"/>
    <dbReference type="OrthoDB" id="10261470at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22948">
        <property type="entry name" value="Coatomer_WDAD_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000002">
        <property type="entry name" value="Coatomer subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000022">
        <property type="entry name" value="Coatomer subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006692">
        <property type="entry name" value="Beta-prop_COPA/B_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047312">
        <property type="entry name" value="Coatomer_alpha_WD-assoc_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016391">
        <property type="entry name" value="Coatomer_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010714">
        <property type="entry name" value="Coatomer_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF1">
        <property type="entry name" value="COATOMER SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04053">
        <property type="entry name" value="B-prop_COPA_B_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06957">
        <property type="entry name" value="COPI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003354">
        <property type="entry name" value="Coatomer_alpha_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <keyword evidence="4 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="49"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="91"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="133"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="207"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="251"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B second beta-propeller" evidence="12">
        <location>
            <begin position="346"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="14">
        <location>
            <begin position="628"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer alpha subunit C-terminal" evidence="13">
        <location>
            <begin position="852"/>
            <end position="1200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR003354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF06957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ62238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1202" mass="135250" checksum="E4945D59FA4E022A" modified="2017-01-18" version="1">MKMLTKFESKSSRAKGVAFHPTRPWILVSLHSSTIQLWDYRMGTLIDRFEEHEGSVRTVDFHPTQPLFVSGGDDYTVKVWSLDTRKCLFTLSGHLDYVRTVYFHHDLPWIISASDDQTIRIWNWQNRSEIACLTGHNHYVMSAQFHPSEDLIVSASLDQTVRVWDISGLRQKHSAPQNNFYEEQYGRQNISQQDIFGNTDAVVKYILEGHDRGVNWASFHPRLPLIVSGSDDRQVKLWRMSETKAWEVDTCRGHTNNVLSVLFHPHQDLIISVGEDKTIRVWDLNKRTPVKQFKRDHDRFWLIASHPHINLFATCHDSGVMVFKLDRERPAHSIFQNQLFYVNNDRQVQIFDYDKQVSSLPTLSLKKIGNAWNNLRTLSYNPSSRSILVTTGEQYALIGLPKDITGAIEPQDLKLGDGNNAVFIARNRFVVYSKSTQNLEVKDLDNSTTKTIKLDSNVKEVVYGGPGSILLLKNNSVVHYDAQQKKVLAEVQVNNVKYVSWSLDGQYIALLSKHTITIVNKKLEVITSMHETIRIKSAAWEETGVLFYSTLNHIKYTLLNGDNGIIKTLDSTLYITKVSGRDVYTLNRNGEVEIVKIDSTEYRFKRALVNKNFYEVLRLIRTSKLVGQNIIAYLQKSGYPEVALQFVQDSQTKFELALECGNLDVALQEAKNLNSAQIWEKLGAEALRQGNGSIVELVYQTQHQFDKLSFLYLITGDFNKLAKMETIAEHRGDIGSLIQNTIYNNSTEKRTATFAQAGSLPLAYATAKTNGHEDLAQAILEQAGLTEEDIELPEIELEPTTKPEVTGHTFTDWPLAPATLSYFEKAILGQLEDLDINDAPADEVETATGSKDVGAFDEPLFEDEDVGGDDGGWDMGDEDLEVEDVNDELDIEIEEGAVPAGELSHWLRNSKVAAGYAAAGAFDTAAQILNRQVGVVNFEPLRSRFLQVYEASKLSLSGSDELPPIKTFIRADIEEDDLNKVLPFLPGFEEITKTINEGFKNFKANNLPAAIESFRKVIYIIAVIAVDNDEDEQKTLEALSIAREYILGLSIELQRRSLGAEEVQRNLELAAYFTKTKLIPSHRVNALQVAMNQSYKHKNFASASYFASEFLKIVTSGPRAEQAQKIKAKSDTIARDAIEINFDPYVDFDICAATYTPIYKGSPSVSEALTGAKYQASEKGKLDKITLITSIGAPASGLRIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAA6</accession>
    <name>A0A1E3PAA6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Inosine/uridine-preferring nucleoside hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_87743</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IUNH family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041556.1">
        <property type="nucleotide sequence ID" value="XM_019186128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAA6"/>
    <dbReference type="STRING" id="683960.A0A1E3PAA6"/>
    <dbReference type="GeneID" id="30203374"/>
    <dbReference type="OrthoDB" id="432381at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008477">
        <property type="term" value="F:purine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006152">
        <property type="term" value="P:purine nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="entry name" value="Ribonucleoside hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023186">
        <property type="entry name" value="IUNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304">
        <property type="entry name" value="INOSINE-URIDINE PREFERRING NUCLEOSIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304:SF25">
        <property type="entry name" value="INOSINE_URIDINE-PREFERRING NUCLEOSIDE HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="Nucleoside hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133759" description="Inosine/uridine-preferring nucleoside hydrolase domain-containing protein" evidence="4">
        <location>
            <begin position="19"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="5">
        <location>
            <begin position="24"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="372" mass="41023" checksum="59A1CC6C02119A94" modified="2017-01-18" version="1">MKFQSLLSIAAVLASASAKKIFIDNDGLAPLQLLFPIAAGHEVLGLSTSFGSASLVDGAGQAYDILNTYNLSSCVPHYIGAQQPLLRTYETFHAWEDLFGALVWQGAYDPHYKDSYSWENITYNDTLAGAIALIDAVKSNKDTDPVIVYAAGMMTTVAQALSIYPDLAKDAQGLYIMGGYFDNQYAQTIGDSITIDINTDINLIQDPEAAQIVLTADWDEIVIGGNVTNYLVPSQKLYDILEEKAGGLDVIRSNPYFAQVSKLLATGNYSQNNDQETLPFWDEVVSAYMSWPELILETTNASVAVDTSFYSPFYGSLRMWNPEWAPKSGYKIANATIIDKIDDEKFYGLLIDTFFKNWTQYCEVDGAVPLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAA8</accession>
    <name>A0A1E3PAA8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosomal protein L44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_82054</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL42 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041089.1">
        <property type="nucleotide sequence ID" value="XM_019185323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAA8"/>
    <dbReference type="STRING" id="683960.A0A1E3PAA8"/>
    <dbReference type="GeneID" id="30202569"/>
    <dbReference type="OrthoDB" id="2967263at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.80:FF:000001">
        <property type="entry name" value="60S ribosomal protein L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000552">
        <property type="entry name" value="Ribosomal_eL44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053708">
        <property type="entry name" value="Ribosomal_LSU_eL42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10369">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36A/L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00935">
        <property type="entry name" value="Ribosomal_L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01172">
        <property type="entry name" value="RIBOSOMAL_L44E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="12198" checksum="8945FCF3E882CC21" modified="2017-01-18" version="1">MVNVPKTRRTYCKGKECRKHTQHKVTQYKAGKASLFAQGKRRYDRKQSGYGGQTKPVFHKKAKTTKKVVLRLECVVCKTKAQLSLKRCKHFELGGDKKQKGQALQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAB5</accession>
    <name>A0A1E3PAB5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_60416</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041563.1">
        <property type="nucleotide sequence ID" value="XM_019182987.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAB5"/>
    <dbReference type="STRING" id="683960.A0A1E3PAB5"/>
    <dbReference type="GeneID" id="30200233"/>
    <dbReference type="OrthoDB" id="17845at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031998">
        <property type="term" value="P:regulation of fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039245">
        <property type="entry name" value="TYSND1/DEG15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21004:SF0">
        <property type="entry name" value="PEROXISOMAL LEADER PEPTIDE-PROCESSING PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21004">
        <property type="entry name" value="SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="514" mass="57183" checksum="319471593877829D" modified="2017-01-18" version="1">MSDWVYSPISIHLSDSNHTYATSGVYLFNILTAEYLILSVTGLSNLSSFSFRFTANELINHEYISWVPLELQDVRYFKAQNHQDSFSEFVKGLVLCDGFTFKPTGIENLCLLTFKIPSLSDPNVYPLFAPRYLLPLNPIDGSAVKIPSNVAIDCCPFSLTNVSIFINYKHHGTLNYKFRNGLGYFSDCRYFDDIVGGLVKLDNGEDQSIGLVAGSLVKTNGDGSLLTVVSWSSIFELLNYPQINLNSHRDTASKPKHNYLEDIPESVVRISIQTINDENFWGSGVILSNDFIVTNEHVMRYNELRSLTITTANGKTFSLFDVDLIPSPIIGYDLYFLKIRPSSFDIKPADLCNTYKSQIESLRIGAQVTSVGNGLFFSRYTNQLTPLHSVGKITDLISIEIDNGFQELAMILTSAGCWNGSSGGGLFNKKGELIGIMTSNGKLSNGEIVPNFTLVIPITIIEKCLFMIKNGIDSVDASEKIKDLWMLKSNHLNFYMANVPAQTNNGAPKTPYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAN3</accession>
    <name>A0A1E3PAN3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome b-c1 complex subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Complex III subunit 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_27152</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The complex plays an important role in the uptake of multiple carbon sources present in different host niches.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein, 2 core protein subunits, and additional low-molecular weight protein subunits. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with cytochrome c oxidase (complex IV, CIV).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion inner membrane</location>
            <topology evidence="1 11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UQCRQ/QCR8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041680.1">
        <property type="nucleotide sequence ID" value="XM_019181344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAN3"/>
    <dbReference type="STRING" id="683960.A0A1E3PAN3"/>
    <dbReference type="GeneID" id="30198590"/>
    <dbReference type="OrthoDB" id="6683853at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.210:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.210">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004205">
        <property type="entry name" value="Cyt_bc1_su8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036642">
        <property type="entry name" value="Cyt_bc1_su8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12119:SF2">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12119">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX UBIQUINONE-BINDING PROTEIN QP-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02939">
        <property type="entry name" value="UcrQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81508">
        <property type="entry name" value="Ubiquinone-binding protein QP-C of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="95" mass="10741" checksum="05F1881FE302E68A" modified="2017-01-18" version="1">MGGAPHAKAYMGWWGSLGSPAQKGITTYAVSPFAQNVLKGVAHNAIFNVFRRAKNQAFFIIVPGVIIWEWWAHARDYNEYLYTKAGREELERVNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAU2</accession>
    <name>A0A1E3PAU2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Vacuolar fusion protein MON1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_88129</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Required for multiple vacuole delivery pathways including the cytoplasm to vacuole transport (Cvt), autophagy, pexophagy and endocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endosome</location>
            <location evidence="4">Multivesicular body membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 4">Prevacuolar compartment membrane</location>
            <topology evidence="1 4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Vacuole membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the MON1/SAND family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041743.1">
        <property type="nucleotide sequence ID" value="XM_019186140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAU2"/>
    <dbReference type="STRING" id="683960.A0A1E3PAU2"/>
    <dbReference type="GeneID" id="30203386"/>
    <dbReference type="OrthoDB" id="272411at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035658">
        <property type="term" value="C:Mon1-Ccz1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032585">
        <property type="term" value="C:multivesicular body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990624">
        <property type="term" value="F:guanyl nucleotide exchange factor inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044395">
        <property type="term" value="P:protein targeting to vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043972">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043971">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043970">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004353">
        <property type="entry name" value="Mon1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13027">
        <property type="entry name" value="SAND PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13027:SF7">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN MON1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19036">
        <property type="entry name" value="Fuz_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19037">
        <property type="entry name" value="Fuz_longin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19038">
        <property type="entry name" value="Fuz_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01546">
        <property type="entry name" value="YEAST73DUF"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="FUZ/MON1/HPS1 first Longin" evidence="6">
        <location>
            <begin position="142"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="FUZ/MON1/HPS1 second Longin" evidence="7">
        <location>
            <begin position="308"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="FUZ/MON1/HPS1 third Longin" evidence="8">
        <location>
            <begin position="436"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="31"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF19038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="546" mass="62154" checksum="E705A92329F9CD49" modified="2017-01-18" version="1">MEQDEISIDNDYITGPEYRPQMPKRPQKKPSLSQLTVGQPRSSLRLSELQAQPTTELSTQNIGKEENFLNTDRIYNTLNYYDSSHDPNFDASTIRTSTPTLTIYSDDEELENTLGDLISQPSSEMRLHFNHEEEEASLKGAKQFFILSSAGKPIYSMSSDLDHNDENFINYNGIIQTIVSSFILSGSELRSFVTKTSRFTILNQSPIVLLAISKIGESEMDLLNQLDLLYSFLLSALSKPHIMRSFQNKEGFDLRKHLGRADLSGLDSLTKEIINFNPGILIGALQCIKLKRSVRDKSRQIMLNRRSKNLLYGMLVAPGGKLINVLRPKSHTLHTTDLQILFSIVFNQSKNYSEDEELWLPICLPKFNPNGFLYAFVKFVDSVALILISADKNCFFEMSETSTKIINEFRKHEILSHISDSLKHGLSTIDIPAPLIYHFIYKSKKHLQYVMPTSTNVTALQKYYLQLYSSVDRNGKISVSYIKWEKDGKSPNKSIAGLGWITPNYEVYLITGSITKREVLVNSARGIVSWCRKYQERLFVCEGAIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB10</accession>
    <name>A0A1E3PB10_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_88279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041813.1">
        <property type="nucleotide sequence ID" value="XM_019186144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB10"/>
    <dbReference type="GeneID" id="30203390"/>
    <dbReference type="OrthoDB" id="3981284at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="235" mass="26297" checksum="9B55CED3F6015785" modified="2017-01-18" version="1">MAKKLSEGRKAGSGRKPGTAKTLAEGRKVGSGRKKGSGGKTIGKTLAEGRKAGSGRKKGSKGTPKSDDANSTHSDDSSLLSNLNDVQHQQQQQQIQQAQAQQHQHGHHQLLTHQDLINNQHQQQVPNLSNYHPQLAQQSYLNSIHFIQNQDHNQQHQPQGQNQFPSLANFQQHHQPHQILNAQQQVQAQSPTQVQQRIAQDDRMKYNDIKLIINDLNSKNTIENAILPFNRSRQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB57</accession>
    <name>A0A1E3PB57_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytokinin riboside 5'-monophosphate phosphoribohydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_88332</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041828.1">
        <property type="nucleotide sequence ID" value="XM_019186145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB57"/>
    <dbReference type="STRING" id="683960.A0A1E3PB57"/>
    <dbReference type="GeneID" id="30203391"/>
    <dbReference type="OrthoDB" id="414463at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009691">
        <property type="term" value="P:cytokinin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014074">
        <property type="term" value="P:response to purine-containing compound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000018">
        <property type="entry name" value="Lysine decarboxylase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005269">
        <property type="entry name" value="LOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031100">
        <property type="entry name" value="LOG_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00730">
        <property type="entry name" value="Rossman fold protein, TIGR00730 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31223">
        <property type="entry name" value="LOG FAMILY PROTEIN YJL055W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31223:SF70">
        <property type="entry name" value="LOG FAMILY PROTEIN YJL055W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03641">
        <property type="entry name" value="Lysine_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102405">
        <property type="entry name" value="MCP/YpsA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="25155" checksum="06F6B30793651D6A" modified="2017-01-18" version="1">MTASTKPEKVVCVFCGSSFGNDSTYSKHASELGKQIAEKNWGLVYGGGTTGLMGAVAKSCSENNGYVHGIIPEALVSRERVDPDDFNGKLHEDINNHKGLTPISNEYGKTTIVDDMHTRKRLMGQEADAFIALPGGYGTLEEIMEVITWSQLGIHEKPIIFYNIDGFYDGLLEFIKSSIKAGFISASNGEIVVVGKNTDEVLNHIANYKIPEGRYNLKWENLGTHTHKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBJ3</accession>
    <name>A0A1E3PBJ3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_87584</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041464.1">
        <property type="nucleotide sequence ID" value="XM_019186119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBJ3"/>
    <dbReference type="STRING" id="683960.A0A1E3PBJ3"/>
    <dbReference type="GeneID" id="30203365"/>
    <dbReference type="OrthoDB" id="6251714at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004090">
        <property type="term" value="F:carbonyl reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052588">
        <property type="term" value="F:diacetyl reductase ((S)-acetoin forming) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031132">
        <property type="term" value="F:serine 3-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045149">
        <property type="term" value="P:acetoin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000047">
        <property type="entry name" value="NADP-dependent L-serine/L-allo-threonine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42901">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42901:SF1">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
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        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="269" mass="29274" checksum="66FC03B45EE83529" modified="2017-01-18" version="1">MSYGKQALSRIAGKTIFITGASSGIGKATALEYAEASNGDLKLILVARRLEKLETLKKELNLKFPSLQTFIGKLDVSEKNSIQPFLKNLPIEFQDIDILVNNAGKALGNSKVGEVSNEDIYGTFDTNVFGLISITQAILPIFKKKNSGDIVNIGSIAGRDAYPGGSIYCASKFAVRAFTESLRKELIDTKIRVIEIQPGAVETEFSIVRNYGDAQAASSVYKGREPLFADDIAELIVFATTRKQNTVIAELLVFPSDQASASHVYKKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBY7</accession>
    <name>A0A1E3PBY7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_103642</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042136.1">
        <property type="nucleotide sequence ID" value="XM_019180387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBY7"/>
    <dbReference type="STRING" id="683960.A0A1E3PBY7"/>
    <dbReference type="GeneID" id="30197633"/>
    <dbReference type="OrthoDB" id="2264294at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001080">
        <property type="term" value="P:nitrogen catabolite activation of transcription from RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901717">
        <property type="term" value="P:positive regulation of gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901714">
        <property type="term" value="P:positive regulation of urea catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN DAL81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="199"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="58"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="128"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="766"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="10"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="58"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="129"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="766"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="797"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="847"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="869"/>
            <end position="881"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="897" mass="101450" checksum="8752CE158AD57855" modified="2017-01-18" version="1">MSQIPDDRPQTNTTNLDANNGNIQPNDSNKGATDYWGTDMLSPEFNFLNVPEETYNFLQSGPSLNDRNAPSPSLFNNNTGISNIPSNPSSAQGTPVESNAPAKTEQQNFLLNHYQNLLNQSKVPMKQALSPNSYASPTANSTNSHTAGSPSSNIPINHPLHPDNVQKSLQQQQQQAQSFQQPQSLFQEASQTTKGNPNPCDHCRRRQIKCITVPNLSNCVQCETKGIKCTHSESPSNPALKRNMGMVQDTNEFNKRPRYSVNQVQKPTFPQVQNFKKPQPTLQYPRSSFFVGSSSMFDHSLLNRIRLDKIDQVQLNPKLSLRKVSNDVQFILRDDYTDELALSADRNVDAVENIVAPHGQSLVNTYFKIIHPSFPILHRKVFLEKYARSYREFSAPLLAAVYSLAIQWWDQDPNLAQHQKPDIDALNSIALQTFTEVTERPRLSAVQAGLLLLRCRSENPNNWILCQQVVSLAEELGLGLDCQNWKLPKWERGLRRRLAWAVWIQEKWTSLIESRVSHFVIGRNWLVRNVTNEDFPEHPSRRQNQGDEDYKSMINDIENGKYLFKEMIELSLIMGEIMETFYSYSALQNVTRIDQVLKSAKPLQLQLRQWYHSLPPGLQMNAVQQEKLCSNGYLHLSYFATEITLHRKIILSLGEDTPPELVKVCRAAATTRLTAVIDFVKSLKREHIYAYWNAPTTSNFAIVGTFAAVLYVTAPSPQEASVYREHINVYRMLLYKLSRFFKQAFEALKRIDMLLNHIPGLIKEPSQMNEMSPLSNMSQSPNNNFQALSPYLSQQMNANLPNTQNMPNNQPDMNKNNNNINSNNNNSNNVNRRSGPPVTTVKKESFTEPSNLSPSAMSESARSFKNGGTPVTNTNRSNANSPVDIRMSGNAGQTPNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBY8</accession>
    <name>A0A1E3PBY8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_77086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042133.1">
        <property type="nucleotide sequence ID" value="XM_019184497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBY8"/>
    <dbReference type="STRING" id="683960.A0A1E3PBY8"/>
    <dbReference type="GeneID" id="30201743"/>
    <dbReference type="OrthoDB" id="2852960at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="11"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="555" mass="65191" checksum="D891EF3A439FD7E8" modified="2017-01-18" version="1">MDHQLLDQCPISKLMLLPNEILQNVVSLLTQPDLLTLTLTSKLFYKLSTRILYSKIYLNDSIIEYSDVYELASSWSWLRLQPSSSNEDSRKEANYKLECLLRSLKQDQALVNNIIEVRLNWDLDIQLQKNFVDFLTNSSRSLKIIENVTDPELNEAIVSSNYKSNLISLDLPPPNPLPSLEITDDYLPNAQSYMIRRLTPNIKVLTLFIDPLLLFNNLIRPKKILEIETLKLHCRADTYPGILYNRRHWKYNKLSEIFDSRYLKVLTIISWYDHICPDRIYKFDQWAEFPNLEDITLIAVTYNDKKISNLINNCRQLKRLKLDFSFPKRAMAKNSLVYKSIFTHKDHLKFLDIKLNLTNRIVNLDVRQFTIVFKVPCKCSLCQETVLKGIFEGKILPNPEDYKIDTMETFEKLDYFDQLFDSSLSPYSKAVDRYPSVKTGPDNIEDFLHRFNQVNMERSPNFIPLVEQDFYDLYHCLIHSLKMDVEPFLTHFKELKFLVVNDISLIIQEDETTGMRYPVPVFYNKGFKTNFDVTKSGTNQFEVDSEVYGRSLYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC90</accession>
    <name>A0A1E3PC90_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_61058</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome b5 family. MAPR subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042222.1">
        <property type="nucleotide sequence ID" value="XM_019183103.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC90"/>
    <dbReference type="STRING" id="683960.A0A1E3PC90"/>
    <dbReference type="GeneID" id="30200349"/>
    <dbReference type="OrthoDB" id="899at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000003">
        <property type="entry name" value="membrane-associated progesterone receptor component 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF115">
        <property type="entry name" value="BINDING PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G06240)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="3">
        <location>
            <begin position="25"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ63015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="125" mass="14090" checksum="83879807D2DC6C2E" modified="2017-01-18" version="1">MSTTSTSKFAPKTPVQLDPPKYDPITKKELAEYDGVQKPQRYIGVKGVVFDVTKNIQAYGPDSKYGVFVGRDASRALGKSSLKEEDVDLKISDDISDFTEKQLKVLDDWFSFFSQRYNIVGKIVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PDC8</accession>
    <name>A0A1E3PDC8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_60997</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTH2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042159.1">
        <property type="nucleotide sequence ID" value="XM_019183091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PDC8"/>
    <dbReference type="STRING" id="683960.A0A1E3PDC8"/>
    <dbReference type="GeneID" id="30200337"/>
    <dbReference type="OrthoDB" id="1733656at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02430">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1490.10:FF:000001">
        <property type="entry name" value="Peptidyl-tRNA hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="entry name" value="Bit1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649:SF11">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="Peptidyl-tRNA hydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="32"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="213" mass="23036" checksum="DD4E8FB164BA2135" modified="2017-01-18" version="1">MSSNLQIITACAISLATGLYLGHYFTPTVNIKSSDSKKRHQKQSSQRQPSSSSSSPSSDQITDESDLEESDLEESEEEDFQVDSTSLNDIPGETKMALIVRTDLKMGKGKAAAQCAHAALGAYRLMLNEGQSSQNLPLLKRWEHSGQAKITLQVPNKEDMDLLFAKAISLNINSYIVHDAGRTQIAAGSATVLALGPAPKLVLDQVTGDLKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUE7</accession>
    <name>A0A1E3NUE7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="10">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_65879</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="10">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="10"/>
    <dbReference type="EMBL" id="KV454229">
        <property type="protein sequence ID" value="ODQ56650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035857.1">
        <property type="nucleotide sequence ID" value="XM_019184007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUE7"/>
    <dbReference type="STRING" id="683960.A0A1E3NUE7"/>
    <dbReference type="GeneID" id="30201253"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07849">
        <property type="entry name" value="STKc_ERK1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000040">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000073">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="15"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ56650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="352" mass="41127" checksum="81684D43BFEA4B3B" modified="2017-01-18" version="1">METQRKISFNVSDYYQIQEIVGEGAYGVVCSAIHKPTGTKVAIKKILPFEKPMFCLRTLRELKLLKHFNNHENIISILDIQKPLNFESFNEVYLIQELMETDLHRVIKSQRLSDDHCQYFIYQTLRALKSMHSANVLHRDLKPSNLLLNSNCDLKICDFGLARSIASSENNFGFMTEYVATRWYRAPEIMLTFQEYTTAIDVWSVGCILAEMLSGKPLFPGRDYHNQLWLIMEVLGTPLMEDYYNIKSKRAREYIRSLPFRKKIPLNTLFPEGTNPLAIDLLEKLLTFNPQKRISVVDALNHPYLQLYHDPEDEPDCAPIDESFWEFNENNEGLDMQELKKLLYEEVMKPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUN5</accession>
    <name>A0A1E3NUN5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_65737</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036062.1">
        <property type="nucleotide sequence ID" value="XM_019183977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUN5"/>
    <dbReference type="GeneID" id="30201223"/>
    <dbReference type="OrthoDB" id="10566505at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ56855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="172" mass="20591" checksum="6731F0D6C7EFFFCD" modified="2017-01-18" version="1">MSSERYNQLRRIFQSSDELNNTLETKYHQNLEHQVSKYTQFLNVAKTHQRELDPQDEIVGLHLSNDKIPFHFERGDTIGISLANTVFSEQVKQSEEYVIYLEQQNNQLIKEIEDKHEVNGKLEIYINEVNKRIEQDEGPKDLMGELVEQVQDEKIRYRKLRKILRQLIQEYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUV6</accession>
    <name>A0A1E3NUV6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GST C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_71440</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Glutathione_S-Trfase_N"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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            <end position="318"/>
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        <location>
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            <position position="101"/>
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            <end position="141"/>
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    </feature>
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        <source>
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    <sequence length="342" mass="39658" checksum="C352C1C247241B57" modified="2017-01-18" version="1">MSQSTKQGLKILEFAGKDGAYKRKASVFRDVISRKADSKFKPEAGRYHVYISLACPWAHRVLIYLTTKGLIKDLNPQTTEVSKDSIIGLSVVHWSMDEKGWRFPSEGDLAAAATPDHLYDFKRLSELYYKAQPDYEGRFTVPVLWDKKTKTIVNNESAEIIRFLNTEFNDLIDDPEKKNLDYYPLDLQSKIDSLNTLTYENINNAVYKSGFATKQEVYESEVTNLFVHLDKVEKLLKENNEKGLKFLTGNKLTEADIRLFTTIIRFDPVYVQHFKCNIGTIRSDYPHLHKWVRETYWNNPLIKDTVNFEHIKFHYTKSHIGINPHKITPLGPIPNILPLNHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="match status" value="1"/>
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    <keyword evidence="1" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="40"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="GIT Spa2 homology (SHD)" evidence="3">
        <location>
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            <end position="120"/>
        </location>
    </feature>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="1017"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
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        <location>
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            <end position="1054"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ56993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1229" mass="137009" checksum="8166C7501BA0F50F" modified="2017-01-18" version="1">MSDLQPDHYRDLLTYHGALKQFLSVSNASAKANPKRAAKAREKLLKLSATQFYELSTDVFDELERRIDESREEPDFLLPKSTFHPKRNEAREKLGSLQQGRFRDLVSDIFYEIERRDYHKTQQRASYHSFDYGGQRSSKGSNLNNDLNPSPANGNINKNGVVGLQSTTVIPTKAELAWSSDEGEDMDEKNNDKQHTIIQNQKSFSPSTSSPYHSPQKQQQYHQHQHSTDYQQLQSRSNRNSDLSSRNRNSNPSDKDVLSEMVDSAPKHSTAQVNRGLSLSKTRNKDREIELLLEEGTKMDQTITQLEQKIALLQSKSDKFENETKDLNKINQNLSSKVSSLEGDLSNKIEQLKQLEQNQKSRSLDDDNDKLPPTRNLQVDTNSGKIDQSALDELKNEVLDWRTKFENLKLQKIEDLLSIKTISQSSISNLLDNNGLIPLNLVNILHKSIESFLISLNKDKSAKSKTDVNTLFDHISKISNISGKIASFAPHSDKAELIRASISHSITSARYYALYSEFLPKLVVESAVSEIAFSVCDLISEVKLNKDDSKYSASEFELPNTALNGDEDVSNEISPVRPLRMTKKSFESPSSKNEHNTTTSSTTPRPLNLTFNSQGNNISTPSTSKKPYQGLNGLTSPITSAKKMEFGNKISPSSSSRDLKSNNDASGSNPSTPTTTTATMKKNAFPSVISRYSPESDRKSPESNTTTAPSLRKSSSSNILSKVRQFEQQGNGNGTVSPQRSVVSPFSDSKFGSGVRRTSVDSQPESIEVPKESNDFKRPAETVKTTDVKMNDSLKTSNEVPEVKNVEENIDKNTNQVSNQPTSKDSKISSLPIPVEAKGINYDDGEDDDDDDDEVDFTSIDKLKGAIKKNADLKPKPLKDELEEPIAVEDRAPKEEDDDEDLNELPNEEEYNNVENKTAQNPSELNKDVISKELRGDGLEHHNGNDGLKIERKQLNQAFVEPPSNLAASSKSIEPPIDNTVKSLNLGEHKATSIIEENLNPNPKSSDNGAQISQDDFKSAHEAVLSEDEQDEDGHDVQLKSQGTTTDVKSNGRNYRSEGRDLEEDFDVGQFDIEDPDNTLSELLLYLEHQTVKVISTIQTLLTSIKAADSTKGELRSGSRAINAVVSQMVDATSNSMNQSRNGQLREHGIWVVQSLEDSGRRMDMLCNSSSKDGEDNEDDNEDYADKHFKQRLTGIAFDVAKCTKELVKTVEEANLKEEIEHLDARLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV91</accession>
    <name>A0A1E3NV91_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pantoate--beta-alanine ligase</fullName>
            <ecNumber evidence="3">6.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Pantoate-activating enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Pantothenate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_35998</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(R)-pantoate + beta-alanine + ATP = (R)-pantothenate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pantothenate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036265.1">
        <property type="nucleotide sequence ID" value="XM_019182384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV91"/>
    <dbReference type="STRING" id="683960.A0A1E3NV91"/>
    <dbReference type="GeneID" id="30199630"/>
    <dbReference type="OrthoDB" id="2020436at2759"/>
    <dbReference type="UniPathway" id="UPA00028">
        <property type="reaction ID" value="UER00005"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004592">
        <property type="term" value="F:pantoate-beta-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00560">
        <property type="entry name" value="PanC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000013">
        <property type="entry name" value="Pantothenate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1300.10">
        <property type="entry name" value="Pantoate-beta-alanine ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00158">
        <property type="entry name" value="PanC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003721">
        <property type="entry name" value="Pantoate_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042176">
        <property type="entry name" value="Pantoate_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00018">
        <property type="entry name" value="panC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21299">
        <property type="entry name" value="CYTIDYLATE KINASE/PANTOATE-BETA-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21299:SF1">
        <property type="entry name" value="PANTOATE--BETA-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02569">
        <property type="entry name" value="Pantoate_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ57058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="301" mass="33506" checksum="185DD3A863CE71D7" modified="2017-01-18" version="1">MSDIVVIRTVKELREWKRQIAGGSVGFIPTMGALHQGHLSLVSESLRRNDRTVVSIFVNPSQFAPGEDLDSYPRTFDQDYSILSSVEIEGRKVDVIFAPTVTEMYPSGIDLDTTKQKGAFVSVLGVSEQLEGKTRPNFFRGVATVVTKLFNAVQPDFAYFGQKDIQQTIVLKRMVKDLLLPVEIVVMPIIREDTGLALSSRNTYLSQDTRSKSSMIYKALQAGVHYYNDKKAPVERGEIIRQVTDGLKDPLFKIDYVSVADKEELQELDVVVPGKGAVISTAVYVTENGLTVRLIDNVMLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVE8</accession>
    <name>A0A1E3NVE8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Polyadenylation factor subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_35889</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for 3'-end cleavage and polyadenylation of pre-mRNAs. Also involved in chromosome segregation where it has a role in chromosome attachment to the mitotic spindle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036325.1">
        <property type="nucleotide sequence ID" value="XM_019182367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVE8"/>
    <dbReference type="STRING" id="683960.A0A1E3NVE8"/>
    <dbReference type="GeneID" id="30199613"/>
    <dbReference type="OrthoDB" id="16717at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180010">
        <property type="term" value="P:co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045245">
        <property type="entry name" value="Pfs2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836:SF0">
        <property type="entry name" value="PRE-MRNA 3' END PROCESSING PROTEIN WDR33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="136"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="177"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="219"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="356"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="430"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="430"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="461"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="478" mass="54072" checksum="81E055908011E555" modified="2017-01-18" version="1">MASYERPERQSYYNPQSIETQLASQQKRSTAHRRIIDHGASFGRWSIYKKLNHKQPKLGHIRPEASYTIDLLPVIDNKKEPIIDTPTKFVHLSSNKAKHAIHCIKWTPEGRRLLVASHSGEFTLWNGMTFNFETIMQAHDTAVLSLEYSHNDDWLLSGDQDGVLKFWQPNFNNLNILKAHDDAIRDVSFAPGDGKFVTASDDSSLKIWNFSNGQEERVLKGHNWDVKCADWHSSLGLIVSGSKDNLIKLWDPRTGNCISTLHGFKHTITKTKFQPSQDSYLLSSISRDRSMRIFDLRTMKDLFIYRSEVDLSSLAWNPIHHEMLTTGGYDGSMNHYNLNNLVPDTLETIKPFHTIPYAHEKAIHSLEYHPLGHLLTSAGADRSARFWCRGRPNDPNVFNDPPYTNDKVGAWYFAINNAVNAVVPANENVGKQVGNSNNSNNGYNDRRSYNLPGLDGGDYDGGNNSNNSNGFNLPGLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVI5</accession>
    <name>A0A1E3NVI5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_97559</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nitronate monooxygenase family. NMO class I subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036257.1">
        <property type="nucleotide sequence ID" value="XM_019186591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVI5"/>
    <dbReference type="STRING" id="683960.A0A1E3NVI5"/>
    <dbReference type="GeneID" id="30203837"/>
    <dbReference type="OrthoDB" id="10265891at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018580">
        <property type="term" value="F:nitronate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04730">
        <property type="entry name" value="NPD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004136">
        <property type="entry name" value="NMO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42747">
        <property type="entry name" value="NITRONATE MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42747:SF3">
        <property type="entry name" value="NITRONATE MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03060">
        <property type="entry name" value="NMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="291" mass="32186" checksum="57C3D553218EA55C" modified="2017-01-18" version="1" fragment="single">MKSQLSSFFKTFDVKFPIVQSPMAGASTVELAAIITNAGGLGSIPLGSASENPQVIENQVLKFQSLVQDEKLKRNMNLNFFTHEPPRLDQDVVHQEWLKKFHGYYKKEGIEISPKAESLTCPYPTFKSIDSADHQTIQTLIRLKPKVVSFHFGLPNLKVLKTLQDAGIKVFISATSLQEFLEISEAGADGVVLQGWEAGGHRGNFVPNDIEDEQLTTEELVEKVVDFIDTNHQRFASGDKAPFIVAAGGLYDGETIATVLNYGIAAVQLGTIWLPTKQATISHEHRNLFFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVP6</accession>
    <name>A0A1E3NVP6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_107514</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLI C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036391.1">
        <property type="nucleotide sequence ID" value="XM_019180506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVP6"/>
    <dbReference type="STRING" id="683960.A0A1E3NVP6"/>
    <dbReference type="GeneID" id="30197752"/>
    <dbReference type="OrthoDB" id="3214149at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF8">
        <property type="entry name" value="GLIS FAMILY ZINC FINGER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="156"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="189"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="53"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="100"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ57184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="413" mass="47389" checksum="BDA9051423B4CEB7" modified="2017-01-18" version="1">MDVSAITNHDTANTEPGAAKETELKQSQDHAFSISSMMNNDEERTVSPDPIQTEEREQENGKEENGIHHSDSQNDLVPKDVDAGSESTLSDVESTKAQSEDEFTDLELSDDNAEEIESKRIEADCHWSGCQDKFLNIDDLVQHLNSKHITNVDSVYRCEWDNCFRKGVTQPSRFALISHLRTHTGEKPFFCIIPECSKHFTRADALSQHIRTVHQTSNLSVEYPFWYSYLNGLKNDETFAKRFRLMPEMHSLERYSKLDSGDQFQKELANKKIQFDNIHNIELTDLTEPTSKRQKTSLKDINAGLKASFEKEDADFKHHIAAAAKDTFKSLEGVESLPTDASIESVQSLEELQALHESLRRKYVWGLEVEKLITKELKQLRTEKTQLWLKKETLLDAHIELEISENRDLYSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVV3</accession>
    <name>A0A1E3NVV3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_65595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="480"/>
            <end position="577"/>
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            <end position="398"/>
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        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57339.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
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    </evidence>
    <sequence length="577" mass="64754" checksum="B44DBC8F4BA6888A" modified="2017-01-18" version="1" fragment="single">EFLTKATTKLHIVYRTWLSKTVFKETNNSNFAFKLNSTKDFGLNLNNTKNFTAKLLAIRINFKLTQFQDTASVIKHVLEKNSWKKIGGPYLPTKNISTNGVGGTEYIVATTEESNPKPFLGDKNNLRINIINSDITRCTNCNSLEHKVDFCLERCAYCYGRKHRWKKCAFRAADQKRVRNNEAPKFKGLYSQNNFRPTSDETGNKSNRTQNFDTTTGKHNSNNNGKPSQTEDEDGFVTNNSPKQRRRATQQHDDKPETVAHSNLFNNLPEEAEEEEDDEPMEVEEDLGTEQATSNKPTSTKPTLNSETPIFNLPKQNGKTKRSSRSPTRSNRAKTQAKTNDATNNDEWNPGPSSYFGNITHMHQTEDAPRKTTASGGEPTGARMNDERGKNGSDDLNRGDNGSDVLNRGEKGSDVLNRGENAGGDRNDIAEDGGNSGDTRGGAFNPEGIETMEDGNGFDDEANDIMEDSIVMDDEQIEIENMKDGQNGNQEIETEENDSMNDEEEFSDEPAFDEEPETWYDSTMDESMLEIEETNDSETRNPLPNNYSHGRVLRSQSRQLQNNNEVSDIADAPSNNL</sequence>
</entry>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
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                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
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                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
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                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
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        <property type="project" value="EnsemblFungi"/>
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<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
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                <person name="Pangilinan J.L."/>
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                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
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                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
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            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
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        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <end position="508"/>
        </location>
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        <location>
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            <end position="131"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="436"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="113"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ56787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="524" mass="58963" checksum="709076D21C267FE2" modified="2017-01-18" version="1">MASVEDYPYDQSMFKFSNPPTASGTIGDLNGFGFPTNQIEYFNLGQPPMNLEPMINHSYDLASSTMDPLSNVSPSIEQHQQQPPLQQPVFLSNANLLDLNQTQQIIQPQLSHPLSSTDDSSSSLTPFDQSVSSSTAQFSNSGFISEDSVFPPVDAFIPSSIDTQHQQQQHQFHLQKQNQVQGVKQEHFQRSRYLSEPNIMTSTSNDSPFSLSPQSITFGSNPPTSATTITTTTTLYDSNVKPLYQRYRVIRGISSGGSATKPPRIMTTANHHYVPVNLELLDANLQECCLPTWNEEEKADKRRIIRIERYQVGSKIIAKFSIVGSAKENPTPLESQPGVDVVEVSCLECFTKQSEDTDNEEESGNSTPMNNYSYGSTNTNGVEETKRSFYITSVEVIKIVELLIGTEVQDPAERRRERGRIRSNLVPFWSKRPISSRMSNNEMRSNSSSPKSESNNSFNQISNISPDYSDFRIELATRIMAYDVRKPRGFDKEVRILKWEKLIPALRRALQSYYAEIPDGQDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW53</accession>
    <name>A0A1E3NW53_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_71346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036014.1">
        <property type="nucleotide sequence ID" value="XM_019184239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW53"/>
    <dbReference type="STRING" id="683960.A0A1E3NW53"/>
    <dbReference type="GeneID" id="30201485"/>
    <dbReference type="OrthoDB" id="2553626at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="494"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="566"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="587"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ56807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="619" mass="67895" checksum="C6DC0084A511DB27" modified="2017-01-18" version="1">MNGAGFDWLNIAGLSSDSNLPPPPVSFGVTPTSSTSSVDLVSQQQQQHQREKSTSISQKSQSEDNLATAQPSYVETDADTSVPLSLTVDQLTKDEAKTYLRWYNDIVVRRNTKLIRLKDVFNFLSNFKLNEHVRNILERIFRSCSTLNIGEFFALLRLISHAIMGQIIPARRLIKEPAPIPKPKSILSRKRMNDVENDDDDNDKEGSNNEGNDNNSIATVAAASNADSSKFDLDSFTQFILTGERPGTEDSSLRRRKKNALSKRVKISDEVSVEPSAEASSPPVNLDLTLPMDQLIGRFPKHEPKHEKNINEEEELKEMQDSFDHFKNIKNVDSALIHGTPANIPSIFFDNEMSQNNNSPRVASPDPSSQVRLLSPNHTGPVNTQGLLPPNHTGPAGNSGLLQPTTTGGGDEFLNSLLSPNNTTGHQQQQSLPHSNGYLSPNYTGQANEAPLQPTMTGSVAPSMRQSFGLNQRVSSASPSFGNSNNLSVPMPPQSRQRSLSSPIPFDPSLQQRVTSPISSTRSPPPPPPPRARANTTTSQAPPPPPSRNSHPGPALPPKIPMQNTSSPSPSFYQQQQQPFQQNNPYVHNNDSTSSSTADILGDLKALQSEVDRLQYYQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWZ0</accession>
    <name>A0A1E3NWZ0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_35131</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036851.1">
        <property type="nucleotide sequence ID" value="XM_019182271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWZ0"/>
    <dbReference type="GeneID" id="30199517"/>
    <dbReference type="OrthoDB" id="10262656at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17330">
        <property type="entry name" value="MFS_SLC46_TetA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF15">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="436"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="472"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="503"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="19"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="537" mass="60757" checksum="E9F40FA83025E91F" modified="2017-01-18" version="1">MPNPKESFRTQMKGFPWSQIFVIACVRFAEPIAFTSLFPYVYFMVKDFGISQNEAEVSKYSGYLSSIFAFCQVLTSFNWGKFADKNGRKPTLIIGLTGSIASLLLLGFAKNYYYALAARSLMGLLNGNVAVIRTMIGEVATERKHQALAFSTMPLIFQIGCVVGPLIGGFLSGDSSRFKFLKPLVKKYPYALPNLFVSGLLLVSMITAIFFLEETHYKHKYRYDPFVEFGDAIKRHVFKIEPKKRAWHKNQIRETPPSPAEIDEETALLDDEGDDEQSITSVGHLLTRRESVALIRTYSLHEVKDEDEINYKELLTPHIFYAVVCNFIMSLHIVVHDEFLPIFLAYDIAKSKTGELISKFPFKIVGGLNYSSEDTGQLLSSTGILGVVFVLFVFPYVDRNYDCLTTYKNFIKIFPIVYFLVPYLVLFGDFKTLSKIAAYTLTCLKTLGTSISFPQILLIVHNSSPPKHRAMINGATISVSALARCSGPLIWGFIMSWGQKFQIAWIGWWLLSLLALLAIYQSRYLKDSTEEEGIEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXD8</accession>
    <name>A0A1E3NXD8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_81154</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037033.1">
        <property type="nucleotide sequence ID" value="XM_019185185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXD8"/>
    <dbReference type="GeneID" id="30202431"/>
    <dbReference type="OrthoDB" id="3981298at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="292" mass="33806" checksum="0428BECDA605DB59" modified="2017-01-18" version="1">MNSGSGENGYSVNGKLRHTNREKLFELHRKLHNDKMNENVIENEPIQLVDEKPPRIRRSDVRKYLGDPLPLPYLDYSTRDETSSKVGESNDRSNHVTNRIKFQTRRPLGQNHVENEDGDIYKEKLKESLKKFQRSTASSTSRKLVDRVVQTEDSSNEPITPQGNSYNYTKVNDYKSPTLSLEGSPNGLNYRIMLETFNDKDFIINNFKSRNPQINQPFVSTPNSKSPPRTTLLEPFQPPINKPKLKKADNKNEYIHIRLLSILGSPLFLLGLIFLLYYNLQNHLQREFKDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXP7</accession>
    <name>A0A1E3NXP7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14 15">Diphosphomevalonate decarboxylase</fullName>
            <ecNumber evidence="3 14 15">4.1.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_34346</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(R)-5-diphosphomevalonate + ATP = isopentenyl diphosphate + ADP + phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:23732"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57557"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.1.1.33"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:23733"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 15">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14 15">Belongs to the diphosphomevalonate decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.33" evidence="3 14 15"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037135.1">
        <property type="nucleotide sequence ID" value="XM_019182175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXP7"/>
    <dbReference type="STRING" id="683960.A0A1E3NXP7"/>
    <dbReference type="GeneID" id="30199421"/>
    <dbReference type="OrthoDB" id="10253702at2759"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00100"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004163">
        <property type="term" value="F:diphosphomevalonate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000018">
        <property type="entry name" value="Diphosphomevalonate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.890:FF:000005">
        <property type="entry name" value="Diphosphomevalonate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005935">
        <property type="entry name" value="Mev_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029765">
        <property type="entry name" value="Mev_diP_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053859">
        <property type="entry name" value="MVD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041431">
        <property type="entry name" value="Mvd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01240">
        <property type="entry name" value="mevDPdecarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977:SF3">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18376">
        <property type="entry name" value="MDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22700">
        <property type="entry name" value="MVD-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015950">
        <property type="entry name" value="Mev_P_decrbx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 15" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 14 15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 14 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="11 15" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8 15" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-1207">Sterol metabolism</keyword>
    <feature type="domain" description="Diphosphomevalonate decarboxylase-like N-terminal" evidence="17">
        <location>
            <begin position="10"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Mvd1 C-terminal" evidence="16">
        <location>
            <begin position="195"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR015950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF18376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF22700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ57928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="396" mass="43926" checksum="FE8B92392DA17413" modified="2017-01-18" version="1">MVVYTASTTAPVNIATLKYWGKRDKDLNLPTNSSISVTLSQDDLRTLTSVATSEEFKQDKLWLNGKEESLNSERTQNCLKDLRSLRQELEQSDSSLPKISSWGLHIVSENNFPTAAGLASSAAGFSALVVAIAKLYKLPQSTSEISKIARKGSGSACRSLFGGYVAWEMGDAQDGSDSKAVEVAPLSHWSNLKAAILVVSADKKDTPSTSGMQTTVKTSDLFQWRIKEVVPKRFEEMKKSIIDKNFPVFAELTMKDSNSFHSTCLDSYPPIFYLNDTSKKIIKLVHSINEFYGETIAAYTFDAGPNAVIYYEDFNESKILSFLYQLFDKVEGWSKKFSQDELNKFSSEFNSNSSKIQFQLDDELYKGVSRIILTKVGDGPQDTNESLVGADGFPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXQ6</accession>
    <name>A0A1E3NXQ6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_80052</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037092.1">
        <property type="nucleotide sequence ID" value="XM_019184995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXQ6"/>
    <dbReference type="STRING" id="683960.A0A1E3NXQ6"/>
    <dbReference type="GeneID" id="30202241"/>
    <dbReference type="OrthoDB" id="6365676at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043709">
        <property type="term" value="P:cell adhesion involved in single-species biofilm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000218">
        <property type="term" value="P:negative regulation of invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000221">
        <property type="term" value="P:negative regulation of pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001382">
        <property type="entry name" value="Transcriptional repressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF19">
        <property type="entry name" value="YY2 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="165"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ57885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="203" mass="22986" checksum="43EA9AE32091CDA8" modified="2017-01-18" version="1">MSLIDINYQHTRPTNITLPPISSIIKSLESSLPQHHVLSSSTSPIPAASESTGGLITNNNNIPTLIPKDSYTHYTYSTARSNSSDSLSSSPSSQLLHQPIQPIIQHQQPIIQIPDNIVPIQQEQINQDLNPTIKRKYTCKTCSKSFTTSGHLARHSRIHTGERKHVCPFPNCESRFARQDNCMQHYKTHLNNKLRKRKYNKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY17</accession>
    <name>A0A1E3NY17_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_34228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037316.1">
        <property type="nucleotide sequence ID" value="XM_019182167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY17"/>
    <dbReference type="STRING" id="683960.A0A1E3NY17"/>
    <dbReference type="GeneID" id="30199413"/>
    <dbReference type="OrthoDB" id="294251at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000026">
        <property type="entry name" value="TBC (Tre-2/Bub2/Cdc16) domain family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="109"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="456" mass="53488" checksum="4B9189F7257907B9" modified="2017-01-18" version="1">MDKLNKSATANDLETIQSSRKSASKIRLSIEESQTKIGSNRDRYGFKKKSNFVSELEYNDWWVDYSKHCARRKKKWETLMIKSGLSLDNDNPERFPARSEKLKRYIRKGIPAEWRGNAWWYFAKGQDKLNSNKGLYDKLVESTVNMHNKDTEIIERDLNRTFPDNIHFRPERTSSTVTPETPLIQALRRVLVAFATYQPSIGYCQSLNFIVGLLLIFMDEEKAFWMLVIITSKYLPGVHEINLEGVNIDQGVLMLTIKEYLPETWQKIISINFDDSIDPNSEHYDFLVKLPPVTLCTSSWFMSAFIGILPIETTLRVWDCFFYEESSFFFKTSLSIFKLLEPRLIVHHDEMEVFQIIQNAPKSIIDPSTLFDLIFKKKHGFNNLTQEDINRCRKFVGERRQKYKESSDINDANFNRGLLNESLWQPDVYGFKKGLPGVHWNKNLTRRMRNLRKKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYD9</accession>
    <name>A0A1E3NYD9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_106506</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037322.1">
        <property type="nucleotide sequence ID" value="XM_019180476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYD9"/>
    <dbReference type="GeneID" id="30197722"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="123" mass="14245" checksum="61C3B100B43204C0" modified="2017-01-18" version="1">MHMKLLSFDYNYLFTTHTKLTIYPSLIPSIDAFLNLQDYDLLSIILVLDLTICEDICHHVERFAMNFTDIEAINLQVTLVNTLLDKSFYSTAWKLPLHAIAFIQMLKTAHLLTPQTIKTIEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYU7</accession>
    <name>A0A1E3NYU7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CN hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_64531</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037598.1">
        <property type="nucleotide sequence ID" value="XM_019183757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYU7"/>
    <dbReference type="STRING" id="683960.A0A1E3NYU7"/>
    <dbReference type="GeneID" id="30201003"/>
    <dbReference type="OrthoDB" id="201515at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008418">
        <property type="term" value="F:protein-N-terminal asparagine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070773">
        <property type="term" value="F:protein-N-terminal glutamine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039703">
        <property type="entry name" value="Nta1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11750">
        <property type="entry name" value="PROTEIN N-TERMINAL AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11750:SF26">
        <property type="entry name" value="PROTEIN N-TERMINAL AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="338" mass="38327" checksum="61EBFBB18757BD89" modified="2017-01-18" version="1">MSKLRVALLQLNPVIHKLEENIHRADTILSKLPSNKPIDLLVLPEFAFTGYKFPDAQTLSPYLEKTSKPGPSISWAQETSQRLNCFTVLGYPESQGQTTFNSAIVINPNGEIIHNYNKSFLYDADDQFGASEGEGFKTFEINGIKASIGICMDLNPYKFKAPFNKFEFSSHCLENDVKLVICPMAWLHSKSPSINEELSKDEKLKLAGKFKAQIEEIKGEYQIDTNGDKIENFEELKEFEQLKEPDYNNLNYWLLRFFPFMNHMMKPYYYEDKATILFCNRSGVEDDVLFGGTSSIVQFLGTKGLVGYNSIDLTNKSVDVLGALGKGNEGLLYREVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYV1</accession>
    <name>A0A1E3NYV1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Large ribosomal subunit protein mL67</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Mitochondrial homologous recombination protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_33545</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL67 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037397.1">
        <property type="nucleotide sequence ID" value="XM_019182086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYV1"/>
    <dbReference type="STRING" id="683960.A0A1E3NYV1"/>
    <dbReference type="GeneID" id="30199332"/>
    <dbReference type="OrthoDB" id="5333655at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024629">
        <property type="entry name" value="Ribosomal_mL67"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28184:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28184">
        <property type="entry name" value="MITOCHONDRIAL HOMOLOGOUS RECOMBINATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12829">
        <property type="entry name" value="Mhr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="197"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="210"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="227" mass="26929" checksum="8EDD5B42D65DDE90" modified="2017-01-18" version="1">MSNRYRPASWLRKNEFAPQVFLFRNMESGQVLYSQLPTFTQYQIKRQFKRPNWENRKPSTRPDIWRIMAVATFPDYEAAIKAYDSLVQLRNMRDISKKDEAMKYRPRNEDNHIWYSAQYRPIYTQEAVADLNTVVEHIQKLTTIHWEDEWRKGDLKHWNGDLVKHELLDRTGLKTPVALLDQLRIKGREAFQKLSMLENSQRQKEKSAAKLESSASAEQKQQPEASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYX3</accession>
    <name>A0A1E3NYX3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_64350</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCP11 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037417.1">
        <property type="nucleotide sequence ID" value="XM_019183715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYX3"/>
    <dbReference type="STRING" id="683960.A0A1E3NYX3"/>
    <dbReference type="GeneID" id="30200961"/>
    <dbReference type="OrthoDB" id="276323at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010737">
        <property type="term" value="P:protein kinase A signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008862">
        <property type="entry name" value="Tcp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832:SF11">
        <property type="entry name" value="LD23868P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832">
        <property type="entry name" value="TESTIS-SPECIFIC PROTEIN PBS13 T-COMPLEX 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05794">
        <property type="entry name" value="Tcp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01017">
        <property type="entry name" value="Polyprenyl_Transferase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="128"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="28"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="143"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="961" mass="109271" checksum="DE09E9D712EB535A" modified="2017-01-18" version="1">MPQAPTATQESTHAKRARLDSTISLNQPPTSTPTNTNSTTTNTDQVNSVISAGPSTPSATAHIHHHHHHPIPHSTTASNDSTEDGKLDIPPLSFETSQNSFISTPTACPSLEEDHHHHHIIEKKVQVVVPPSTTTTASSSTNQKRKHKPSSSKIRKYRSRSLPNILFSSKNLDFFQSKIKDPSTTNIFSTTTSTTSTPPAPLPPVNIQSLREIDLAEILKNPQLRHDILFDPQLQFRPNLDGERGRRKKVTYDKYWSGVQLEIEEYYQNQHVFSKNSSRLPNLFQTLRDILISLLPSKDKQTVIDVLDIELIMQQLSNHSLDFVKLANWLSSIFKSHCAPMRDSWVDEMLTKFVEADESKSVLKLVEGLRMIFTILEAMKLDVANHQIRILRPVLVETAVEFERDYFLQMIARCKLDISDSVKWFQQFNNSETTPRIITTQAILSLLSCSKMVSEFPSSLAFDHARLIVLRANVRQVVCLQLCIALFKQLCHQSNNNQLITNESIESLKKEILALITDENGNVKWTRNIGAIALQISRRSNPLMIIPNEKTVEFAFSWLIKQTQPSSQVYSLMEQRVFNIIKEKVVDLEHEKLNNVMPVSGNGNVAVASATTTTTTTNEEDDIGSIASRISLLAKISLQRANKRSIEMESNIEEIIKTPQWSDQKENLITAPYDYLTTTPGKHLRTLLIQLFNTIYKVPPIQIEQISKVIEILHTSSLLIDDIEDDSNLRRGKPTAHLIFGIPYTINSSNYMYFKALNCLLQLDPKLVDVFNEEMLNLHRGQSLDLYWRENLSCPSEEEYINMVMNKTGGLFRLSVRMMEHFSQEETKISLIPLANLLGIIYQIKDDYKNLKNDELQLNKGFCEDISEGKLSFPIVHSLNKNPMDQTLLGILKQRTHNDDIKKFALSYLEKTDSFGYTERSLKYLGDKAINLIREIMIKTGENSDGFKNIENLVLKLIETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYX6</accession>
    <name>A0A1E3NYX6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glucose-signaling factor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_80540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037603.1">
        <property type="nucleotide sequence ID" value="XM_019185082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYX6"/>
    <dbReference type="STRING" id="683960.A0A1E3NYX6"/>
    <dbReference type="GeneID" id="30202328"/>
    <dbReference type="OrthoDB" id="4076669at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022757">
        <property type="entry name" value="Gsf2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11055">
        <property type="entry name" value="Gsf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="43259" checksum="B5C7080E49009656" modified="2017-01-18" version="1">MSDPADVIDAQDAVFEIYVRMNEEHEKDYCFNVGINDTFESLFKIFDSLKISLRPNIFHEQRPIGFKVSDSPGYLTDNGGLLFSYNADKFTKNVKLTDKISDHCWPGHLVLPIWREDYFTHYAIISLILVWLYTDLPDFISPTPGICLTNQISRALARVAVRFDYPQLAADLITETTPNSTSKTAQIIFFIFHIVKSLMIYLVLYSGLFNPYCLNPIKMHKIASNDKLDEKKKLELISLGWTGTRKATLDEYKEFYREYKIKEVGGIVKASKMGLFEKLKEPGVNLKFGEGFQSDLSNKTTLKDLEANPEKFILNFDWFALVGENYENYLATESTDKAQDVKNFRRYGPLVASDRIKKIVEGRKDKPELLKLAEGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYZ0</accession>
    <name>A0A1E3NYZ0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lipoyl synthase, mitochondrial</fullName>
            <ecNumber evidence="10">2.8.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Lipoate synthase</fullName>
            <shortName evidence="10">LS</shortName>
            <shortName evidence="10">Lip-syn</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Lipoic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_101592</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine + 4 H(+) = [[Fe-S] cluster scaffold protein] + N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 5'-deoxyadenosine + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:16585"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:14568"/>
            <dbReference type="Rhea" id="RHEA-COMP:14569"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:33722"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="2.8.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the radical SAM superfamily. Lipoyl synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.8" evidence="10"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037560.1">
        <property type="nucleotide sequence ID" value="XM_019180325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYZ0"/>
    <dbReference type="STRING" id="683960.A0A1E3NYZ0"/>
    <dbReference type="GeneID" id="30197571"/>
    <dbReference type="OrthoDB" id="3231at2759"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00593"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016992">
        <property type="term" value="F:lipoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000036">
        <property type="entry name" value="Lipoyl synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00206">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031691">
        <property type="entry name" value="LIAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003698">
        <property type="entry name" value="Lipoyl_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00510">
        <property type="entry name" value="lipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004019">
        <property type="entry name" value="PRK05481.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009544">
        <property type="entry name" value="PRK12928.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949">
        <property type="entry name" value="LIPOYL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949:SF0">
        <property type="entry name" value="LIPOYL SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16881">
        <property type="entry name" value="LIAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005963">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00271">
        <property type="entry name" value="lipoyl_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01058">
        <property type="entry name" value="lipoyl_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="7 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="12">
        <location>
            <begin position="115"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ58353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="379" mass="42113" checksum="ADEEFFA9A7EF2E45" modified="2017-01-18" version="1">MLSQKLIKSEVSKDNASVDGVNPAPKRRRRTVFSDALNKGPSFDDFVSGKAADFNIDPIEAARVDPKARIPSWLRVPIPKGESFHNLKKDVRELKLATVCEEAKCPNIGECWGGKKSEATATIMLLGDTCTRGCRFCSVKTNRKPAAPDPMEPENTAEAISRWGLGYVVLTTVDRDDLIDGGSNHLAETVRRIKQKAPQILVEVLSGDFRGDLEMVKILASSGLDVYAHNVETVEALTPHVRDRRATFRQSLAVLNTAKETNPDLITKTSIMLGLGETDEQIMDALKELRAINCDVVTFGQYMRPTRRHMKVAEYVKPEKFDYWKQVALDMGFLYCASGPLVRSSYKAGEAFIENVLRKRGNNQVNTDSIQSHTVTTNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ44</accession>
    <name>A0A1E3NZ44_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">Imidazoleglycerol-phosphate dehydratase</fullName>
            <ecNumber evidence="4 9">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_106637</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="4 9"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037481.1">
        <property type="nucleotide sequence ID" value="XM_019180479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ44"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ44"/>
    <dbReference type="GeneID" id="30197725"/>
    <dbReference type="OrthoDB" id="447729at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000005">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000001">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="entry name" value="Imidazole glycerol phosphate dehydratase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00076">
        <property type="entry name" value="HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020565">
        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002114">
        <property type="entry name" value="PRK00951.2-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133:SF2">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00954">
        <property type="entry name" value="IGP_DEHYDRATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00955">
        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="224" mass="24176" checksum="497E19F94756147E" modified="2017-01-18" version="1">MSANGTSNERKATISRITNETKIQIAVSLNGGPVTIEKSILKDKSYDATQSTSSQVISINTGIGFLDHMIHALAKHGGWSLIVECIGDLHIDDHHTTEDVGIALGMAFKEALGQVRGVKRFGTGFAPLDEALSRAVVDLSNRPYAVIELGLKREKIGDLSCEMIPHFLESFAQAAHITLHVDCLRGFNDHHRSESAFKALAIAIKESISSNGTNEVPSTKGVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ72</accession>
    <name>A0A1E3NZ72_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_64676</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037748.1">
        <property type="nucleotide sequence ID" value="XM_019183782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ72"/>
    <dbReference type="GeneID" id="30201028"/>
    <dbReference type="OrthoDB" id="10354108at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="86" mass="9734" checksum="3A43B372856D07A7" modified="2017-01-18" version="1">MSQNNNSSVQPLHPILDSKENVTKARVHELAVQQKLKYVSPSDKKENLSPTSKKLMDHKLKHLYKNPVKKLSGLRLEAAVSSDEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZB5</accession>
    <name>A0A1E3NZB5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_64584</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037652.1">
        <property type="nucleotide sequence ID" value="XM_019183765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZB5"/>
    <dbReference type="STRING" id="683960.A0A1E3NZB5"/>
    <dbReference type="GeneID" id="30201011"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000142">
        <property type="term" value="C:cellular bud neck contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902404">
        <property type="term" value="P:mitotic actomyosin contractile ring contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10224">
        <property type="entry name" value="ASKHA_NBD_actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000148">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000001">
        <property type="entry name" value="Actin, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000404">
        <property type="entry name" value="Major actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="376" mass="41732" checksum="6AC37D05E6DEE81F" modified="2017-01-18" version="1">MEGEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQSKRGILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKTNREKMTQILFETFNVPAFYVNIQAVLSLYSSGRTTGIVLDSGDGVTHVVPIYAGFALPHAILRIDLAGRDLTDYLMKILSERGYSFTTTAEREIVRDIKEKLAYVALDFDQELQTSAQSSAIEKSYELPDGQVITIGNERFRAPEALFHPSLLGLEAAGIDQTTYNSIIKTDVDVRRELYGNIVLSGGTTMFPGIAERMQKEITALAPSSMKVKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPAIVHHKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZY9</accession>
    <name>A0A1E3NZY9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_63230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037930.1">
        <property type="nucleotide sequence ID" value="XM_019183511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZY9"/>
    <dbReference type="STRING" id="683960.A0A1E3NZY9"/>
    <dbReference type="GeneID" id="30200757"/>
    <dbReference type="OrthoDB" id="284184at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097176">
        <property type="term" value="P:epoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051340">
        <property type="entry name" value="Haloalkane_dehalogenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42977:SF3">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42977">
        <property type="entry name" value="HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="30"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="291" mass="33132" checksum="98FCAF1AC9C0742E" modified="2017-01-18" version="1">MSSITPTVNKIQLKDGTKVFYREAGSKDKPIFLLLHGYPTSSIMFRNLVPLLSQHYHVIAPDLPGFGFTETPKGYDYSFANITDSIDEFISILKIDSFKVYIFDYGAPVAFRLALKNPSRITGIVAQNGNAYEEGIDDRFWGALKQYWAKGDEQDPGYVDALSKFIEDKQNILSQYYEGVSNPNVIDPAPAVLDEFLLKRPGQTKIQLGLFYDYQNNIKLYPQFQSFLRNSKVPVLVAWGKNDYIFTVEGAEAYKRDASKFKAKYYETGHFALETHVEEIAADIIDFFVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0F8</accession>
    <name>A0A1E3P0F8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Dipeptidyl aminopeptidase B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_84571</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S9B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038072.1">
        <property type="nucleotide sequence ID" value="XM_019185733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0F8"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF200">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 10, ISOFORM B"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00708">
        <property type="entry name" value="PRO_ENDOPEP_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="16">
        <location>
            <begin position="155"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="15">
        <location>
            <begin position="592"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006150"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ58865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="797" mass="90810" checksum="F051B9C4BC61E2F7" modified="2017-01-18" version="1">MSNDTDFTDEKVPMRTHDQRYSERKKHSSYKGFVISIGALAALFWGASILVLGIARLRSVTLQIKQLDLLNSSPNVFLRGFNETRNTTFSPKYKSIQWISEPDSLINDKGTFVTSDDDIYVIKSIQDSNYEKLLYNGSHIFQDGYNFSIDSFVASPNLKYALIQTNSSQNWRHSTFGLYWILDVDTQKITQLNPGLVSIAKWAPPSDRIAFVLDNNIYIYELSSGKTTQSTFDGSAEIFNGKPDWVYEEEVLEGDTALWWSPNGEYVAFLRSNDTLVPEFPIPYFVQGDGEEIYPELRKLKYPKPGFNNPEVSIGIIDVQKAKTKLVDYHYDIFTEIIWVGNDQLLLKATNRESDFLKVVLVDAQDLSFEIVRDEVTESWFEITHDTFFVPKSEENGRQHDGYFDTISVDGFNHLAYFTPPNNSTPKILTSGEWEVVSAPSAFDYEKNSVYFLSTQDSSIERHLYSLNLLTGENKKLTEGEGWFSASFSSGARFLALSYRGPEIPYQHLIDLHTGEVEVLEDNKKLSNLLSTYELPTNNYGEVDIGTAKVNYVERLPPNFNSSETYPVLFFVYGGPNSQLVTKTFGFSFPDVVASQLNAIVVTVDGRGTGFKGRKFRSVVRDNLGNYEVIDQVAAAKHWGSKSYVDESRITIFGWSYGGYMTLKTLEYDAGETFKYGMSVAPVTDWRLYDSIYTERYMHTPQNNEDGYIESSVHNVTQIGEATRFLLCHGTGDDNVHIQNSLKFLDKLNLEGVENYDVHVFPDSDHAIRYHNANVIIYDKLLLWLKKAFRGDYINYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0G9</accession>
    <name>A0A1E3P0G9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Extracellular mutant protein 11 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_63384</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0G9"/>
    <dbReference type="GeneID" id="30200783"/>
    <dbReference type="OrthoDB" id="4056678at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029178">
        <property type="entry name" value="Ecm11_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15463">
        <property type="entry name" value="ECM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Extracellular mutant protein 11 C-terminal" evidence="2">
        <location>
            <begin position="193"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="290" mass="32775" checksum="6C7E7D23E9AD6358" modified="2017-01-18" version="1">MTASKSSSSISALNEPKVKVEGVSKPKKHVTIKLEPQTINKSNSTGSSSSSIGPTNKKDGNNSNVDKIPITPNEKILKQPNLKSIEDLNISKFYIQPQQQINNTDDDEDTPTKSTSSKKNDSKRINDIKTPSSHQPIKSKKARSFDQTEITPTNTVNPLKKSKPQPVILKKPSIFKPIEESIIENNNEIDGLKLIEIFSNIDDELIKQKYIESSSKNFENWSIQGFQLINYQYEIVKKIIQSRNKLNLKFQLIFGLINTYGNSLENEDLVLNEKMNKLQTLGEEIKNFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0L9</accession>
    <name>A0A1E3P0L9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_32564</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038233.1">
        <property type="nucleotide sequence ID" value="XM_019181978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0L9"/>
    <dbReference type="STRING" id="683960.A0A1E3P0L9"/>
    <dbReference type="GeneID" id="30199224"/>
    <dbReference type="OrthoDB" id="70250at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000506">
        <property type="term" value="C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007720">
        <property type="entry name" value="PigQ/GPI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21329:SF3">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21329">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT Q-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05024">
        <property type="entry name" value="Gpi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="302"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="331"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="453" mass="52619" checksum="25C82A21BA42BFCC" modified="2017-01-18" version="1">MEKNWETHKLWNTILTKLTNTLFVLFTIFHKLPLKSGKFLGLSIVVGLRHIAMIIIKILNHKIAPEFPSLIELSATAQQIDLRLQQFCYSPIQYIRTTRKIDWNATSLAHMQSPVENSLSIEQYPEYIRFYNTLWLVINDVTLGAITGAVLIENQEILIKVFNEIIVSKILYEDLKKISMWLMNSPGGIKLNNELSMFFSELFTWMIEFWNVSLVDTLVPNLSTVFYILALSSTFGATLSIAIFSDFLSIITFNIYCFYFASARIFNWQLDILASLFHLFCGQKKNILRNRIDSNDYDLDQLLLGTLLFTVLIFLLPTVFAFYLTFTITRLSILVIVGSLEFVMSCLNHFPIFVILLRLKDYRRTPGGIEFENEEDYLRIKSKPLEVSEMFKPFSNVIDKLKSSYFSFKTLKKILTGFPINVQRNKFYKLLYSALPSNHISTSELWSETKKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0V2</accession>
    <name>A0A1E3P0V2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_33653</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037759.1">
        <property type="nucleotide sequence ID" value="XM_019182100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0V2"/>
    <dbReference type="STRING" id="683960.A0A1E3P0V2"/>
    <dbReference type="GeneID" id="30199346"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000065">
        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF28">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="372"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="406"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="448"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="516" mass="58658" checksum="E9861D556D193600" modified="2017-01-18" version="1">MAADSPSKAQENKLIKWLKYMFIWYPANYTTQERKLLFKLDLIILVYGCTSFFAKFLDQTNITNAYVSGMKEDLSLYGNELNWLNITYLSGYVLGQIPFLLLMSRPKFSKYVLPTLEILYGICTFCQSRITNIDQLYALRFLVGLFEAPSFAGVHQILGVWYGSRPYKGNPPELFVRAGTWFLCSSLGSMFSGYLQSAAYRNLSGVGGMRGWQWLFIIDGIITIPIAFVGYAFWPGLPDSGKPWYLNKEEYSLALTRTKRNKIEKPGKLDRSVWKRTFTQWKWYVFVAAYISMMMGYYPTNYFSLWLKAQKKYTVYQINNYPTVTNAVTIISSFTGTALAAVFDPWKIFLVSISGQVIFGIIMIIYDVPPAATFFAFYIAGTAGCSSPILYSSINRILRGDQEQKAIVMGSMMSIGYFVYTWLPLALYPTAASYGERAAPRWKVGYPTQLAFTILLGSFFLLATYLDNRDRINSGETMDVEEDVIDSDESSFSELHDIENGKVHIVTSVNNKSISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P100</accession>
    <name>A0A1E3P100_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate dehydrogenase assembly factor 3</fullName>
            <shortName evidence="6">SDH assembly factor 3</shortName>
            <shortName evidence="6">SDHAF3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_63435</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Promotes maturation of the iron-sulfur protein subunit of the SDH catalytic dimer, protecting it from the deleterious effects of oxidants. May act together with SDHAF1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with the iron-sulfur protein subunit within the SDH catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the complex I LYR family. SDHAF3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038137.1">
        <property type="nucleotide sequence ID" value="XM_019183550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P100"/>
    <dbReference type="STRING" id="683960.A0A1E3P100"/>
    <dbReference type="GeneID" id="30200796"/>
    <dbReference type="OrthoDB" id="278329at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015976">
        <property type="term" value="P:carbon utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006111">
        <property type="term" value="P:regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006105">
        <property type="term" value="P:succinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20270">
        <property type="entry name" value="Complex1_LYR_SDHAF3_LYRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008381">
        <property type="entry name" value="SDHAF3/Sdh7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137">
        <property type="entry name" value="DC11 ACN9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137:SF6">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="126" mass="14891" checksum="0408A4938B84F068" modified="2017-01-18" version="1">MRPTLVNLVKPRRPERPGQVLLPPLTLYRNILRAHKKLPIAQRQLGDLYVKEEFKAHKTTDNPLYIVGFLTSWQDYLNLITKGKWMESSLTSEQLSKMSPDQVNQLYELMKETQNYYKENPKEEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P145</accession>
    <name>A0A1E3P145_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">proline--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Prolyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_93953</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Pro) + L-proline + ATP = L-prolyl-tRNA(Pro) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="2"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038260.1">
        <property type="nucleotide sequence ID" value="XM_019186435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P145"/>
    <dbReference type="STRING" id="683960.A0A1E3P145"/>
    <dbReference type="GeneID" id="30203681"/>
    <dbReference type="OrthoDB" id="10267474at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00779">
        <property type="entry name" value="ProRS_core_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004500">
        <property type="entry name" value="Pro-tRNA-synth_IIa_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050062">
        <property type="entry name" value="Pro-tRNA_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033730">
        <property type="entry name" value="ProRS_core_prok"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00409">
        <property type="entry name" value="proS_fam_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42753">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME PROTEIN L39/PROLYL-TRNA LIGASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42753:SF2">
        <property type="entry name" value="PROLINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="56"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="572" mass="65867" checksum="716D6B3C9715AA84" modified="2017-01-18" version="1">MVFHIARGIRHNSNIATQHLFTAKSIPKTATTHQLLQDLGFIYQPSAGLIHWLPLGLKVLKNVENIIRKRMDEIGGEEFSLSSISSKELWEKTGRWSNTELFKLQLNSKKDKDFCLTPTHEEEITEIIKNANLNYKNLPVLTYQISRKYRFEKRPRGGLLRGREFLMKDAYSFDKDYESAMEMYNKVNDAYFKIFQDLKLPFVRAQADSGDIGGSLSQEWHYVHESGEDLLFKCDHCGDVSNVEKTVSIPDEDTPVAKSAKVAYILTKDQNTLVCAYYPSDRTFAPNFLKDHVEDLDLTTLNTSFEELMKIFIKDEDDLVLNKNFIRVMDSRIDHTTDLPDFPITKFQKNNFSTLTDISIVEAIENEYCDSCETGQLKSMNAIEVGHTFYLGKRYSEPMGAKFTNVDNKMTTFEMGCYGIGVSRIVAAIAELSRDEEGFVWPSSISPYDLSLVEAPKCNQDHVNEVVENLKDLKISFDKRQLNKVGFGAKMINARMLGIPIILVIGKNYPIVELEVRGKRWNESKSKYNYQELYEQNSKDWEWQIIEDGNFEKHLVHKDHVEKVIKSLLKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P164</accession>
    <name>A0A1E3P164_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_84821</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion inner membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038398.1">
        <property type="nucleotide sequence ID" value="XM_019185788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P164"/>
    <dbReference type="STRING" id="683960.A0A1E3P164"/>
    <dbReference type="GeneID" id="30203034"/>
    <dbReference type="OrthoDB" id="274922at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042719">
        <property type="term" value="C:mitochondrial intermembrane space chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140318">
        <property type="term" value="F:protein transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000002">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038:SF16">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="14">
        <location>
            <begin position="18"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ59191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="90" mass="9761" checksum="E9D03ED0CD522819" modified="2017-01-18" version="1">MSFLGFGGAPAQPSDAKIQAAEAELDLVTDMFNRLVDNCHAKCIQKSYSEADVNKNESLCIDRCVSKYFEVNVKVGENLQKIGASGAFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P168</accession>
    <name>A0A1E3P168_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glycosyl hydrolase, family 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">AAM3</name>
        <name evidence="6" type="ORF">WICANDRAFT_32958</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038207.1">
        <property type="nucleotide sequence ID" value="XM_019182017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P168"/>
    <dbReference type="STRING" id="683960.A0A1E3P168"/>
    <dbReference type="GeneID" id="30199263"/>
    <dbReference type="OrthoDB" id="1740265at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004556">
        <property type="term" value="F:alpha-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033934">
        <property type="term" value="F:glucan 1,4-alpha-maltotriohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004574">
        <property type="term" value="F:oligo-1,6-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004575">
        <property type="term" value="F:sucrose alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000025">
        <property type="term" value="P:maltose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005987">
        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11333">
        <property type="entry name" value="AmyAc_SI_OligoGlu_DGase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000064">
        <property type="entry name" value="Oligo-1,6-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.400.10:FF:000004">
        <property type="entry name" value="Oligo-1,6-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000087">
        <property type="entry name" value="Oligo-1,6-glucosidase IMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="entry name" value="Oligo-1,6-glucosidase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF179">
        <property type="entry name" value="NEUTRAL AND BASIC AMINO ACID TRANSPORT PROTEIN RBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0462">Maltose metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="5">
        <location>
            <begin position="16"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="570" mass="66218" checksum="3873CDED4E7059C3" modified="2017-01-18" version="1">MTVSTYPWWKDSVVYQIWPASYKDSNGDGVGDIPGIISTLDHIKDLGANVVWLSPHYASPQDDMGYDISDYEAIYEKYGTMEDMDRMIDEVHKRGMKLIFDLVINHTSSEHAWFKESRSSLDNPKRDWYIWKKPRYDSNGNRIPPTNWKSYFSGSAWEYDDTTGEYYLRLFAKSQPDLNWENEEMRNALYKTSMQFWFNKGIDGFRIDTAGKYSKDQNFEDAPIVFPDQEWQPCHKEVNNGPRIHEFHKEMFSKVTSLYDAMTVGEVGNCTREEAVRYVSSKEKEMNMLFLFDVVDVGSNRADKFRYEGYTLKDFKDAVAAQCEFIKDTDAWSTVFIENHDQPRSVSRFGNTSNKLLTFKSANLLTLLQTTLTGTLFIYQGQEIAMTNVPRSWDISEYKDINTINYWNQFQKDHPNDKEAAEKLLDVIALQARDHARSPVQWDDSPNGGFTTGEPWTRVNDNYKDINVKSQVNDPSSVYSFWKKSLKLRQLHKDIFVFGDFEIVDYANDKIFSYLKTSGDKKAYIVLNFSNETVEFEPQVEGELTLVSSNVDDVKDKVLSAYEGRIYLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P185</accession>
    <name>A0A1E3P185_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_79621</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038295.1">
        <property type="nucleotide sequence ID" value="XM_019184922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P185"/>
    <dbReference type="STRING" id="683960.A0A1E3P185"/>
    <dbReference type="GeneID" id="30202168"/>
    <dbReference type="OrthoDB" id="3979546at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF3">
        <property type="entry name" value="HALOTOLERANCE PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="535"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="593"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="26"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="142"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="718" mass="82295" checksum="22D5C4759512D69B" modified="2017-01-18" version="1">MSDSKVKKKQKSRLVPPESRRRAAFSCDRCKTKKIKCNRTDLDENINKFDKVTPCNNCQKSGVECTTSIPRRRRVYSSFENLGMHYQTLLTLVQGLYPDVDTNDINELIGLGETLGLKMSSGIPQDDLKKFDVESKILKPFSPRFETPSGNSVAGTDDGDGDGGSIQAMNNTPKESHGGPSKPSAPAFATSDTLVFDSKGGSHFIGSSGTPVLFSVLCDLLLKRSYRTNLSFDYELKLFQNRVDNHSKVAISELILKEPNFPMIKFISKEESDYYINFFFRFFHPFYFIFDEQIFRLKYERFWKVLENNSNEDENKEPLSNSYIGCIYLVWLIASKNSDYKTTLDYGKILEVLQIILSDVALASTINSIQFLYLFARYLHSNKNRDSAWNLIGLAIRQAISLGIHKKIMSKKTEHFNLTSQVLWSLYKIELTLCSTFGRPSNINEDEIDIDYPNLDQFPYLDPQFKTYYMTEVKLSRFLNKILKERKSSNGQQPISLINIERTLLMKNQLITISKELQMTPMDQIKTIYDYKLNLVFNYYMVTLTLPFMVYIATTNYKVRTDETLLNIMKTGINASMKCSKLLRLAVDMKFNYGALATDCIYGYSATLVLALFFIYLSNSSNDMLELDIDEHMVDKKVLMECITNVTFFMNSSRLDLTTTRILEVLEGIKNDLKLVEIQDNLKFDVLNFDGSDEVFDSLLMNNDIYTNGFDLGTSIGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1K2</accession>
    <name>A0A1E3P1K2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_23162</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the sal C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038415.1">
        <property type="nucleotide sequence ID" value="XM_019180977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1K2"/>
    <dbReference type="STRING" id="683960.A0A1E3P1K2"/>
    <dbReference type="GeneID" id="30198223"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001102">
        <property type="entry name" value="Transcription factor IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000045">
        <property type="entry name" value="ZFP69 zinc finger protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051565">
        <property type="entry name" value="Sal_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23233:SF84">
        <property type="entry name" value="FI23031P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23233">
        <property type="entry name" value="SAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="74" mass="8583" checksum="222767AA6A38D84F" modified="2017-01-18" version="1" fragment="single">NSSTSSSTLLGMTPETARRNRCTICQKQFKRPSSLQTHLYSHTGEKPFACTWDNCGRLFSVRSNMIRHRKLHER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1L6</accession>
    <name>A0A1E3P1L6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sulfhydryl oxidase</fullName>
            <ecNumber evidence="8">1.8.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_31851</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2</text>
            <dbReference type="Rhea" id="RHEA:17357"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16520"/>
            <dbReference type="ChEBI" id="CHEBI:17412"/>
            <dbReference type="EC" id="1.8.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.8.3.2" evidence="8"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038347.1">
        <property type="nucleotide sequence ID" value="XM_019181890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1L6"/>
    <dbReference type="STRING" id="683960.A0A1E3P1L6"/>
    <dbReference type="GeneID" id="30199136"/>
    <dbReference type="OrthoDB" id="17199at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016971">
        <property type="term" value="F:flavin-dependent sulfhydryl oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160203">
        <property type="term" value="P:mitochondrial disulfide relay system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.310:FF:000003">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.310">
        <property type="entry name" value="ERV/ALR sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039799">
        <property type="entry name" value="ALR/ERV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036774">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017905">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645">
        <property type="entry name" value="ALR/ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645:SF0">
        <property type="entry name" value="FAD-LINKED SULFHYDRYL OXIDASE ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04777">
        <property type="entry name" value="Evr1_Alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69000">
        <property type="entry name" value="FAD-dependent thiol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51324">
        <property type="entry name" value="ERV_ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0274">FAD</keyword>
    <keyword evidence="3 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERV/ALR sulfhydryl oxidase" evidence="9">
        <location>
            <begin position="83"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU371123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="195" mass="21643" checksum="A56BF8B95A50EF72" modified="2017-01-18" version="1" fragment="single">RENMSNESEKKPEFGASGRKIIYDKDGKPCRSCNTLLDFKMANFGTKAKTAGAAAVGSAAIGAGAAAATSSSKTESSVYPKDCPPDVTQLGRSTWTFLHSVAASYPEKPTESQQTNMVQFLNIFAKIYPCWFCAKDFENYISKPETKPKVTNQEEFGKWLCDAHNEVNVKLGKPKFDCNLWKQRWRDGWDDGSCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1Q7</accession>
    <name>A0A1E3P1Q7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mmc1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_63913</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1Q7"/>
    <dbReference type="STRING" id="683960.A0A1E3P1Q7"/>
    <dbReference type="GeneID" id="30200883"/>
    <dbReference type="OrthoDB" id="5319015at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056196">
        <property type="entry name" value="Mmc1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38644">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38644:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23868">
        <property type="entry name" value="Mmc1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23867">
        <property type="entry name" value="Mmc1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="432"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Mmc1 C-terminal" evidence="2">
        <location>
            <begin position="290"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="459" mass="52632" checksum="81EA8CBFBFCEEA5B" modified="2017-01-18" version="1">MLVKRVLVNNLLKSARCCYSTISAQPTVTRASPVIPSIITPKSSPSSTIKTKPSTPTSIKQQIVNLQKVPTHFLNEYGVYEKIDLLIQKLRYPNTPIRIGLLGSEKTFLNTILADPFASDQSWYESLLKRSNGGSLFKYGENPEILSNNYTIPSPFLQTHNIEFLEIHNIEYNDGCHLYINFGSPIDIKWPTFEFKDVDSELVLEANEINSKKSFEAINLLRESPLNSTIYTNLYNSSHFPKFNQALLEIIEPSVIITKNFNSIIKILDHQSISNSLTKTDLELKTQKIQSLIKQWDEYTHYEFQKTFIPFINSFESKQLSWWKLYYKNDDVENLLIRMLDESGFFQNSLQKISYIKGQIDSFNANDLKNSELIEVSNRLNSNSLTKVHNDLISNELLPIQNYAIKLLLINFIGLQLPVVVISSLGWYLYDFSLYSMFGLGSLGLILGLNHSNNHYLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2F4</accession>
    <name>A0A1E3P2F4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of fatty acids protein</fullName>
            <ecNumber evidence="10">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_44856</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an acyl-CoA + malonyl-CoA + H(+) = a 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:50252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:50253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038591.1">
        <property type="nucleotide sequence ID" value="XM_019182584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2F4"/>
    <dbReference type="STRING" id="683960.A0A1E3P2F4"/>
    <dbReference type="GeneID" id="30199830"/>
    <dbReference type="OrthoDB" id="434092at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF157">
        <property type="entry name" value="ELONGATION OF FATTY ACIDS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="286"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="318" mass="37039" checksum="048ABEBEEE5E03EC" modified="2017-01-18" version="1" fragment="single">MSAVEQILGTIQEKTPYYLNLINSNTPNFINVEIPNIETPFGLKLWPVFNEFVSHLTFGKFIPSEFQYIENETFMSSNKEVFIGILIYYFIITIGQLIFKKLSPLKLQFLFQLHNLFLTTASLTLLLLLIEQISPILINHGIFYAICSPNSWNQKLVTIYYLNYLTKFVEFIDTYFLVVKKKKIIFLHSYHHGATALLCFIQLGGATSVSWVPILLNLAVHVVMYWYYFLASRGIRVWWKQWITTFQILQFVLDLGFVYFSTYTFYAEKYSREWGIYIPNNGTCFGTPFAAGTGCAILSSYLLLFIAFYIKTYKSDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2N5</accession>
    <name>A0A1E3P2N5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial import receptor subunit TOM20</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Mitochondrial 20 kDa outer membrane protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Mitochondrial import receptor subunit tom20</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Translocase of outer membrane 20 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_30434</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the Tom20 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038820.1">
        <property type="nucleotide sequence ID" value="XM_019181734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2N5"/>
    <dbReference type="STRING" id="683960.A0A1E3P2N5"/>
    <dbReference type="GeneID" id="30198980"/>
    <dbReference type="OrthoDB" id="2154253at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016031">
        <property type="term" value="P:tRNA import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.10:FF:000002">
        <property type="entry name" value="Mitochondrial import receptor subunit TOM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.10">
        <property type="entry name" value="Mitochondrial outer membrane translocase complex, subunit Tom20 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002056">
        <property type="entry name" value="MAS20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023392">
        <property type="entry name" value="Tom20_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12430">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12430:SF0">
        <property type="entry name" value="TRANSLOCASE OF OUTER MITOCHONDRIAL MEMBRANE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02064">
        <property type="entry name" value="MAS20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037707">
        <property type="entry name" value="MAS20_rcpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00351">
        <property type="entry name" value="OM20RECEPTOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47157">
        <property type="entry name" value="Mitochondrial import receptor subunit Tom20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 14" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR037707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ59613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="169" mass="19217" checksum="8E712C32F520DADD" modified="2017-01-18" version="1">MGKFSYILAAAATLSVGYAIYFDYERRNSPEFRRKLKKSKKHHEKAVIEHEKEVKTVKLEAVKEALIQSMKEGPAIPTDAAEKERFFMTQVTQGEQLSAVPGEELNAAIHFYKALSIYPNPTDILNIYQRSVPAEIYEYIIMLIALQPPQAVANILSESVQLDQEQEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2S1</accession>
    <name>A0A1E3P2S1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_32709</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the rtf2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038701.1">
        <property type="nucleotide sequence ID" value="XM_019182000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2S1"/>
    <dbReference type="STRING" id="683960.A0A1E3P2S1"/>
    <dbReference type="GeneID" id="30199246"/>
    <dbReference type="OrthoDB" id="247013at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006274">
        <property type="term" value="P:DNA replication termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16653">
        <property type="entry name" value="RING-like_Rtf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006735">
        <property type="entry name" value="Rtf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027799">
        <property type="entry name" value="Rtf2_RING-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12775">
        <property type="entry name" value="PROTEIN C20ORF43 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12775:SF0">
        <property type="entry name" value="REPLICATION TERMINATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04641">
        <property type="entry name" value="Rtf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="210" mass="23858" checksum="085A915393358A83" modified="2017-01-18" version="1" fragment="single">DGGVKLQRKDLIKQKKSSEYDEASAEAYNKNGKWTTCHLSNKPLAIPIVSDYKGNLYNKDSILEYILYPDTISTNQKQLVEHIKSLKDIVELKIQTGNDNAEFTCPITGNVIGSNGITYVYLVSCGDVFAEKCLREVNGDDRNCPVCNQQYEDQDVIVLNPISKKSIEHLEDRIQLLISRNLTHSMKKQKKQKKRKSEEAVTHKSKKVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2Y6</accession>
    <name>A0A1E3P2Y6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Non-structural maintenance of chromosomes element 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_78212</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the SMC5-SMC6 complex, that promotes sister chromatid alignment after DNA damage and facilitates double-stranded DNA breaks (DSBs) repair via homologous recombination between sister chromatids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the SMC5-SMC6 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NSE4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038771.1">
        <property type="nucleotide sequence ID" value="XM_019184684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2Y6"/>
    <dbReference type="STRING" id="683960.A0A1E3P2Y6"/>
    <dbReference type="GeneID" id="30201930"/>
    <dbReference type="OrthoDB" id="361242at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027786">
        <property type="entry name" value="Nse4/EID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014854">
        <property type="entry name" value="Nse4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16140">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16140:SF0">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08743">
        <property type="entry name" value="Nse4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Non-structural maintenance of chromosome element 4 C-terminal" evidence="9">
        <location>
            <begin position="289"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="28"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="390" mass="45048" checksum="148E3687783A95AA" modified="2017-01-18" version="1">MLGVKRNGVTTNGVDHGQTKKRVKYAVEDDEEDEAGESSEEEEEEDDDEDDEDDEEEEEEEDDDEQDLQDPNDLLSKEQKLLALSREISSAIKNDRARAAREGGLEQAREHLEKINAAFAERQKLKSSHKTLEVYDSRNLLDGGDLMDIALRNIKLGTSGGVLNVEEFTRRMKTFLLKTAIQYDNEDDDPENYYDKKHEFRKFNWFQFGTFLYSRGNKAPTTSHLLGPLEIEKKIRQFKARVAEIPVGKQVTAEKVSGDDINGKSINQTPNSVQECFKVFHEKSHGAEINIFKFFINPHSFSQSIENMFFTSFLIRDGRLILGEDEDGYPTIAELPPLPQDPKEREKVLLERRDRPSNHIIFQLEYDSWKNLIEKLNITESYLPHRLEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3C2</accession>
    <name>A0A1E3P3C2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_42378</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039188.1">
        <property type="nucleotide sequence ID" value="XM_019182569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3C2"/>
    <dbReference type="STRING" id="683960.A0A1E3P3C2"/>
    <dbReference type="GeneID" id="30199815"/>
    <dbReference type="OrthoDB" id="2238745at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF174">
        <property type="entry name" value="ARRESTIN-RELATED TRAFFICKING ADAPTER 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="227"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="436"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="436"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="461"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="478" mass="54256" checksum="F0249E8D281AA0B4" modified="2017-01-18" version="1" fragment="single">MNNLFPVEKSLDITPETRPLTQTGSIQVYLQLAEPNLFVQGFEPHEYQERPPTILRGSLVLRVLKSSKIRNITLTFKGISKTEWPEGIPPKRQDYVEENDLLNHTWPFYNHANDHNQSGADIVKNSTTATNNSNDSNNINNTTTDPSQHFVFHPGDYIYNFEHAIPASTPESIDATFGSVSYSLNVMIERIGAFKSNIATTLPVRIIRAQSEESVEDSEPIAISRDWEDQLHYDIVIATKAIILNAYIPVAFKLVPLDKIKLHRIRIYLTENLEYYCRNKKVHRMEPVKKYLLLEHKAPPPPDLPPDADAKAKKMGNLLTSDGYDVTAKEFEFQVYVPQKLNNRQNLHPNTSYANIKAHHWIKICLRLSRLIDGKPKHYEISIDSPIHVLHPLCSHANTLLPAYASTLGNRSLPNFESTLSANVYKPENLDIELTSPQAQPVSPSFSPSTRPIHLIRRPSFEPPPFDADISPPPMRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3M1</accession>
    <name>A0A1E3P3M1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_78569</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039150.1">
        <property type="nucleotide sequence ID" value="XM_019184749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3M1"/>
    <dbReference type="STRING" id="683960.A0A1E3P3M1"/>
    <dbReference type="GeneID" id="30201995"/>
    <dbReference type="OrthoDB" id="413079at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051788">
        <property type="entry name" value="MFS_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23514">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23514:SF3">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="433"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="60"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="462" mass="51654" checksum="43F238B49FCADF96" modified="2017-01-18" version="1">MSSKDNQDVELTDFLLGDDSVSQSLQSEKESNVKKTITFKDQPLFINSKDWRDSFMLKAQILTCFFCMITVGLNDQTVGSLIPTLTKQYSVNQTSISTVFALQFLGYATSALSNEILHSNLGRCGVMTLANCFLLITYVVNAFKPPIFIFIAVYYFTGLGVGLLDSCMNVWLSSLVDHNELMGLLHGFYGIGSVISPPLISHILKWFNDDFKIFYIFLAILAITGGLLSQFFFKSESKLKYEYERACAHDDEEGGQEVTLKTVLRNRTVMSFSAYLFLYIGAEVSIGSWLLSYLMKVKGLDQISASYIVSWFWIGLTVGRILLGFVTKFFKNEYRANLTYSALSLFVYLIYTVFTLSTTNKTAHFKLLTNLIMFIDGIFIGPLFPTSSVTLMKILPPNLHVSAVGIMSSLGGSGAAILPFLVGSITHVTKLDYLPLFITAMLTGYVVIWVLMPKLCKVDYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3R7</accession>
    <name>A0A1E3P3R7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CWH43-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_24177</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039145.1">
        <property type="nucleotide sequence ID" value="XM_019181020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3R7"/>
    <dbReference type="STRING" id="683960.A0A1E3P3R7"/>
    <dbReference type="GeneID" id="30198266"/>
    <dbReference type="OrthoDB" id="10032492at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019402">
        <property type="entry name" value="CWH43_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050911">
        <property type="entry name" value="DRAM/TMEM150_Autophagy_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21324:SF2">
        <property type="entry name" value="EG:22E5.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21324">
        <property type="entry name" value="FASTING-INDUCIBLE INTEGRAL MEMBRANE PROTEIN TM6P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10277">
        <property type="entry name" value="Frag1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="CWH43-like N-terminal" evidence="6">
        <location>
            <begin position="3"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="220" mass="25662" checksum="562C419E327CBD08" modified="2017-01-18" version="1" fragment="single">KNYWFMVPIIGFIPWLGMLITMLICWAAQGHPLYPFNSGYQYPVYISYIGATNLRPLFISCAGWQGLFYTITLLVEYFLRKTGRLQYWFKKDERNLLFAACILGIIGSLGILFVAIFSIKNYDNVHHAMLGVFLAFICLSIICLCAQYCMMGLHYRNIHVHHKYWNKFTISFYLKVFFVVAAIIFAICFGAVSNRSVSSCFEWTLAFWYIVIFLIFAWDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3V3</accession>
    <name>A0A1E3P3V3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">Pyruvate carboxylase</fullName>
            <ecNumber evidence="6 17">6.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">WICANDRAFT_105169</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.</text>
    </comment>
    <comment type="function">
        <text evidence="3">Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20844"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 17">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.4.1.1" evidence="6 17"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039383.1">
        <property type="nucleotide sequence ID" value="XM_019180439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3V3"/>
    <dbReference type="STRING" id="683960.A0A1E3P3V3"/>
    <dbReference type="GeneID" id="30197685"/>
    <dbReference type="OrthoDB" id="196847at2759"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004736">
        <property type="term" value="F:pyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07937">
        <property type="entry name" value="DRE_TIM_PC_TC_5S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000003">
        <property type="entry name" value="Acetyl-CoA carboxylase biotin carboxyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000018">
        <property type="entry name" value="Biotin carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000010">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.600.10:FF:000007">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000033">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000012">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.90">
        <property type="entry name" value="Conserved carboxylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.600.10">
        <property type="entry name" value="pyruvate carboxylase f1077a mutant domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003379">
        <property type="entry name" value="Carboxylase_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055268">
        <property type="entry name" value="PCB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005930">
        <property type="entry name" value="Pyruv_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006761">
        <property type="entry name" value="PRK09282.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009554">
        <property type="entry name" value="PRK12999.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01235">
        <property type="entry name" value="pyruv_carbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778">
        <property type="entry name" value="PYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778:SF2">
        <property type="entry name" value="PYRUVATE CARBOXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02436">
        <property type="entry name" value="PYC_OADA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001594">
        <property type="entry name" value="Pyruv_carbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89000">
        <property type="entry name" value="post-HMGL domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14 17" id="KW-0092">Biotin</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="9 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="24">
        <location>
            <begin position="27"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="23">
        <location>
            <begin position="149"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="25">
        <location>
            <begin position="565"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="22">
        <location>
            <begin position="1102"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="active site" evidence="18">
        <location>
            <position position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="574"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="646"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="20">
        <location>
            <position position="742"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="772"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="774"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="906"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="21">
        <location>
            <position position="742"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-biotinyllysine" evidence="21">
        <location>
            <position position="1143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR001594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="ODQ60176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1184" mass="130049" checksum="60999BBB90AD38D5" modified="2017-01-18" version="1">MSFEPVRVPDSGDINKIRRNSLLMSSTTNKILVANRGEIPIRIFRTAHELSMQTVAIYSHEDRLSMHRLKADEAYAIGYKGQFSPVQAYLQIDEIIKIAKEHGVNMIHPGYGFLSENSEFAKKVEASGITWIGPSHDIIDSVGDKVSARNLAAAADVPTVPGTDGPIDSVEQAQDFVNKYGFPVIIKAAFGGGGRGMRVVREGDDIADAFTRARSEAVTAFGNGTCFIERFLDRPKHIEVQLLADNYGNVIHLFERDCSVQRRHQKVVEVAPAKTLPKEVRDAILTDAVKLARTANYRNAGTAEFLVDNQNRHYFIEINPRIQVEHTITEEITGVDIVAAQIQIAAGASLEQLGLLQDRITTRGFAIQCRITTEDPSKNFQPDTGKIEVYRSAGGNGVRLDGGNGFAGAIISPHYDSMLVKCSCSGSTYEIARRKMIRSLIEFRIRGVKTNIPFLLTLLTNDVFVEGLYWTTFIDDTPKLFEMVSSQNRAQKLLAYLADLAVNGSSIKGQVGLPKLDKEPTVPEIHDAKGEVINVAQPPPKGWRDVLLAEGPEKFAQKVREFDGTLIMDTTWRDAHQSLLATRVRTHDLLAIAQTTAHGYAGAFALECWGGATFDVAMRFLHEDPWARLRKLRKLVPNIPFQMLLRGANGVAYSSLPDNAIDHFVKQAKENGVDIFRVFDALNDLEQLKVGVEAVKKAGGVVEATVCYSGDMLQPGKKYNLDYYLEITDKIVAAGTHILGIKDMAGTLKPSAARLLIGSIRAKYPNLPIHVHTHDSAGTGVASMAACAKAGADVVDVATNSLSGLTSQPSVSALLASLDGEIDTNINVGHARDIDAYWAEMRLLYSVFEADLKGPDPEVYQHEIPGGQLTNLLFQAQQVGLGEKWIETKRAYREANYLLGDLVKVTPTSKVVGDLAQFMVSNKLTPEDVKRLAGSLDFPDSVLDFFEGLIGQPYGGFPEPLRTDILRGKRRKLNVRPGLELAPFDFEKIREDLSSRFNKITECDVASYNMYPKVYEDFRKIQEQYGDLSVLPTKNFLAPPKIGEEIIVTIEQGKTLIIKCNAVGDLNKETGIREVYFELNGELRKVSVTDKKSNVETVSRPKADAHNPNQVGAPMAGVLVEVKVHKGSEVKKGDAVAVLSAMKMEMVISAPTAGLVADVLVKEGDNVDASDLLVVIEPQEEEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P414</accession>
    <name>A0A1E3P414_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_78713</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039300.1">
        <property type="nucleotide sequence ID" value="XM_019184778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P414"/>
    <dbReference type="STRING" id="683960.A0A1E3P414"/>
    <dbReference type="GeneID" id="30202024"/>
    <dbReference type="OrthoDB" id="6359943at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47369">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47369:SF1">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="87"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="523"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="539"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="630" mass="71133" checksum="CD0C81B0AA41F266" modified="2017-01-18" version="1">MSHRGLSMRGFMPSAMSPNAHATRRTSLENLMEQTRNVESPSKETGTAYDSENPSADDGDTCPYGCCEKESLSDFILKRGFFEGACSDITIRAFNQDYRLHKLILDRSGYFSSLFNGPWNDPSNTTHELIFEDDENITQESFELAIARLYGAVKPKAEIQHTLSMIAIGQYLDIPEVVCNATDNIVNSLNFDNIARFTSFASMNDYGKASERIIDSAKGLLCSDGWQNGFEKWDGVPVSIIAYVVGHDAFFVPSEWERALFIMKLIERRQSMNRSSSSGSVSELDDEEDVEPLINALNTKVHYCHLSAEQLHKLETYVDNSGKPHIDPSVLKNALWLSVLLYRKVATAEKKSNELGLVTTSEAPPNKENTWFIPTKDETLYGTPQQLEESIKSHHHIRDDMPSGKTTENAGDAVYKVTKVPPFRFSIAFSQVWELEAEKRVYAKTLWYAGSYWNLYIQKIRHRKGYQMGVYIHRASNSAPSRSALLNRDVFKNSTYSLDNDSSLSNIIGDVNNLMLNDEPEASNDTLSSFTKSELNAAPDEEEEEEEEENDNSFLSYEDNRIKTSVYYVIYTPSRKVKTSLTCFISTPDLFNKSQSWGWKSNSMCSFNDDGTLADGQDKLLKFMVVLGNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P423</accession>
    <name>A0A1E3P423_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Crossover junction endonuclease MUS81</fullName>
            <ecNumber evidence="15">3.1.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_29854</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Interacts with EME1 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication fork intermediates. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with EME1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the XPF family.</text>
    </comment>
    <dbReference type="EC" id="3.1.22.-" evidence="15"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039310.1">
        <property type="nucleotide sequence ID" value="XM_019181660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P423"/>
    <dbReference type="STRING" id="683960.A0A1E3P423"/>
    <dbReference type="GeneID" id="30198906"/>
    <dbReference type="OrthoDB" id="5963188at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048476">
        <property type="term" value="C:Holliday junction resolvase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048257">
        <property type="term" value="F:3'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:DNA catabolic process"/>
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    </dbReference>
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        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd21036">
        <property type="entry name" value="WH_MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20074">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000307">
        <property type="entry name" value="Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000003">
        <property type="entry name" value="Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.670">
        <property type="entry name" value="Crossover junction endonuclease EME1, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042530">
        <property type="entry name" value="EME1/EME2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010996">
        <property type="entry name" value="HHH_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033309">
        <property type="entry name" value="Mus81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047417">
        <property type="entry name" value="WHD_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047416">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451">
        <property type="entry name" value="CLASS II CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451:SF0">
        <property type="entry name" value="CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14716">
        <property type="entry name" value="HHH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21136">
        <property type="entry name" value="WHD_MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47802">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 15" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 15" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0469">Meiosis</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="17">
        <location>
            <begin position="278"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="91"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU369042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ60103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="542" mass="62432" checksum="48A5178E12CE95C1" modified="2017-01-18" version="1">MELPNDIKHLYITWLEEDVLAYSLRAQKSALLYTRALEKLRNYPEPVLTPRELIRVPFIGEKIVGQMEKRLEKYCKENGYNLPIGVNSVQRASSGKRTKATLDDNPTNDTAEPKKKRAKKKYVPVKRSGGYAILLVLLEFDPGCSGMTKDAIIKNCTPYCDKSFTANPSTNQFYSAWSSVKSLLNNELVKVEGIPAHYYLTDEGKDLAENLKFADEIVFRTDAPRPSSSSSALRNPGVGDEPIVMDTIPEVRPDMNRVIHNYNSVNYHFWEPGTYTVRFVVDNREVRSSTDRDFFHRKLQQLGVDAESRPLTVGDGLWIARNKTTNEEVVLDYIIERKRLDDLASSIKDGRYNEQKVRLKRSAVKNVFYLIEEVMSSDVQQMADAIQTSMGMAMTTSSFHVKRTKDADDTIQFIAKITKEVVKYYTGKRLLVLEPRNLEFQQDYRRVLDEFREKFGRGNIECAHKYYTFDTMLSKTKLMTVREMFVRMLMTIRGISLEKAIAIQKDYKTPKDLIRNFDGQDPELAKKYGKRVQELIADVFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4D1</accession>
    <name>A0A1E3P4D1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Luciferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_92376</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the NtaA/SnaA/DszA monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039410.1">
        <property type="nucleotide sequence ID" value="XM_019186360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4D1"/>
    <dbReference type="STRING" id="683960.A0A1E3P4D1"/>
    <dbReference type="GeneID" id="30203606"/>
    <dbReference type="OrthoDB" id="5561043at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051260">
        <property type="entry name" value="Diverse_substr_monoxygenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016215">
        <property type="entry name" value="NTA_MOA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03860">
        <property type="entry name" value="FMN_nitrolo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011">
        <property type="entry name" value="ALKANESULFONATE MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011:SF16">
        <property type="entry name" value="C2H2 FINGER DOMAIN TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000337">
        <property type="entry name" value="NTA_MOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="6">
        <location>
            <begin position="42"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="469" mass="52568" checksum="E4E5DA22B6C657B3" modified="2017-01-18" version="1">MTTNTNKRVKLSSQPKKWVINAFLMNTPGLQNTGLWAHPDNQISKYNTIKFWQDLAVKLEDAKFSGIFIADVLGPYDVYKGPDNLTPALVGAAQLPTNDPQLIVPAMAAVTNSIGFGITVSTTYEHPYDLARRFSTLDHLTEGRVAWNIVTSYLRSAARAHGLPDQIDKTIRYERADEYLDVVYKLWEGSWKDDAVVKDKVQNIYADPSKVRYIHHEGKHYKVDGVHLAEPSKQRTPVLFQAGLSPGGKEFASKHAEAIFITGSFPEKVKANVQEIRSKAESKGRDPNSIKFVLGITVVVDETDEAALAKYESYKKYANPEGALALFGGWFGLDFSKAPDNVDLKTLEGPIGDFIRMTRPEKTLTKQEFSLDSSLGGVYPKVIGSATTVADKLQKWIEDTGVDGFNFNYIIAPDTFEDLIKYLIPELRKRGYVDDEYFSPGGTFRENLSRVKGKNHLDEDHYGHSFKWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4D2</accession>
    <name>A0A1E3P4D2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">IMP-specific 5'-nucleotidase 1</fullName>
            <ecNumber evidence="12">3.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_62684</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">IMP-specific 5'-nucleotidase involved in IMP (inositol monophosphate) degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>IMP + H2O = inosine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27718"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="3.1.3.99"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ISN1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.-" evidence="12"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039327.1">
        <property type="nucleotide sequence ID" value="XM_019183418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4D2"/>
    <dbReference type="STRING" id="683960.A0A1E3P4D2"/>
    <dbReference type="GeneID" id="30200664"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006190">
        <property type="term" value="P:inosine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071590">
        <property type="term" value="P:nicotinamide riboside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071592">
        <property type="term" value="P:nicotinic acid riboside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009453">
        <property type="entry name" value="ISN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28213">
        <property type="entry name" value="IMP-SPECIFIC 5'-NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28213:SF1">
        <property type="entry name" value="IMP-SPECIFIC 5'-NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06437">
        <property type="entry name" value="ISN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028836">
        <property type="entry name" value="ISN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR028836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ60120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="418" mass="47457" checksum="0485F19F77E1541E" modified="2017-01-18" version="1">MSSRYRVEYSLKVHRRDGFIEWIKGLLAVPFVLHAVQSGSHAIGDIKTTSDARRRYAEIFFDVEKLIEDQILMEKSGTPQLGRLQQLVPSISGFFTKLPLERAFYIEDERRSISVRRLVCPSFNDIRLILNTAQALALAQAREPLQMVTFDGDVTLYEDGKSLADDAKVIPRLIRLLSNDIIVGVVTAAGYNEKTGEKYYDRLKGLIDAIVQNKDLTVEQKQRFTVMGGESNYLFRYNDDLKNLQWIDPKEWLLDSMSKWDEKDILNTLDLAENMLNSLQRSLCLPATVIRKHRAVGLVPNQGEKLCREQLEEVVLSTQRRLEVSTCAQNIQFCAFDGGSDVWVDIASKDLGVESLQRYFGGIHPKSTLHIGDQFASVGSNDFKARLAGCTVWIANPEETVQILDDLNKYIEEEAAFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4K7</accession>
    <name>A0A1E3P4K7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_68902</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039618.1">
        <property type="nucleotide sequence ID" value="XM_019184146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4K7"/>
    <dbReference type="STRING" id="683960.A0A1E3P4K7"/>
    <dbReference type="GeneID" id="30201392"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13738">
        <property type="entry name" value="Pyr_redox_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00370">
        <property type="entry name" value="FMOXYGENASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="479" mass="54602" checksum="3C5FD3557DFB785B" modified="2017-01-18" version="1">MTVPQLIENGVKVPRKVKSVAVIGAGPAGAISLDALLKENSFDKVKVFERNSKIGGVWSIEKDPVKIDIAPGTTIHDLDPVLGDIPNFDDDIGKIVKTPRSNQYRFVESAGYAGLRTNVPEQLMCFSDEKDWGVDIKQRIDQHYVKVDAVRDYVERYFNRHPRDHIVFKTSLENVSKDYSKPDSKFVLTLRRETDEKDSDGNLIDEWFQEEFDALVIATGHYHVPVIPKVKGLDLIHGKYPEKIVHSKYFKPNVDTYDDEVVIVVGGRISGMDIVTALSKTSKTVYHSKKTIPEVKQKNQGFENIIEKPIIKEIKLDDEGKILVYFGDGSIVENPDRFIYATGYHLSFPFMNNSYPNFTNGTILPDFFEHTIYSKDPLISIVGIPIQAITFRVFEFQAIWVARFLAGKIQLPSLEEQNRWILVRYQIFKNTPAYHSFQDEKFEWPNYITKIAGGVEPLNGAGKKFPIYTQNDKDNIIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4K9</accession>
    <name>A0A1E3P4K9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_31332</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039455.1">
        <property type="nucleotide sequence ID" value="XM_019181820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4K9"/>
    <dbReference type="STRING" id="683960.A0A1E3P4K9"/>
    <dbReference type="GeneID" id="30199066"/>
    <dbReference type="OrthoDB" id="3427at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133676" description="GOLD domain-containing protein" evidence="9">
        <location>
            <begin position="18"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="10">
        <location>
            <begin position="27"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="209" mass="23743" checksum="6FB5422C08A06069" modified="2017-01-18" version="1">MWNPLLALVLIVQLASALHFYTVPGETRCFYEELTKGTVVIGKFDAYVNSRGDAYEAASNLKLAITVDETFDNDHRVVSQKSSSSGDFTFSALDSGEHKFCITPSYVDKRTKVRVFFDLVLAGAETIDSKRQDEVSVLTNKVKQLSNKVQEIQREQKLMREREALFRDQSESTNSKVVRWSVIQLIVLLGTCAWQLQHLKGFFIKQKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4T6</accession>
    <name>A0A1E3P4T6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thymocyte nuclear protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_30229</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039535.1">
        <property type="nucleotide sequence ID" value="XM_019181704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4T6"/>
    <dbReference type="STRING" id="683960.A0A1E3P4T6"/>
    <dbReference type="GeneID" id="30198950"/>
    <dbReference type="OrthoDB" id="41445at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd21133">
        <property type="entry name" value="EVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.590.10:FF:000003">
        <property type="entry name" value="Thymocyte nuclear protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052181">
        <property type="entry name" value="5hmC_binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002740">
        <property type="entry name" value="EVE_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047197">
        <property type="entry name" value="THYN1-like_EVE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14087">
        <property type="entry name" value="THYMOCYTE NUCLEAR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14087:SF7">
        <property type="entry name" value="THYMOCYTE NUCLEAR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01878">
        <property type="entry name" value="EVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EVE" evidence="6">
        <location>
            <begin position="24"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="203" mass="23884" checksum="41DC8B6FAA3B4C99" modified="2017-01-18" version="1">MLVEKPQPKKPAEFPQDPQNNNVRYWLIKSEPLSRIDPKTGKDVKFPLSELQKLEYEQWDGVRNHEAKNNMLNMSKGDILLFYHSNAPVPGIVGEAKVHNEAHPDSTQFNAKSNYYDPKSTKESPRWWCVDVSFVRRFRSKVSLEQLRSNEKLKDMTLVKRGRLSVTPVRKEEYYEVLKMEESKEINDDIDCVIDQKYIKRIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4X0</accession>
    <name>A0A1E3P4X0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial distribution and morphology protein 34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MDM34</name>
        <name evidence="13" type="ORF">WICANDRAFT_62518</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the ERMES/MDM complex, which serves as a molecular tether to connect the endoplasmic reticulum (ER) and mitochondria. Components of this complex are involved in the control of mitochondrial shape and protein biogenesis, and function in nonvesicular lipid trafficking between the ER and mitochondria. MDM34 is required for the interaction of the ER-resident membrane protein MMM1 and the outer mitochondrial membrane-resident beta-barrel protein MDM10.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the ER-mitochondria encounter structure (ERMES) or MDM complex, composed of MMM1, MDM10, MDM12 and MDM34.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Mitochondrion outer membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="10">The ERMES/MDM complex localizes to a few discrete foci (around 10 per single cell), that represent mitochondria-endoplasmic reticulum junctions. These foci are often found next to mtDNA nucleoids.</text>
    </comment>
    <comment type="domain">
        <text evidence="10">Lacks alpha-helical transmembrane segments, suggesting that it resides in the membrane via beta-sheet conformations similar to those predicted for other outer membrane proteins and porin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the MDM34 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039149.1">
        <property type="nucleotide sequence ID" value="XM_019183387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4X0"/>
    <dbReference type="STRING" id="683960.A0A1E3P4X0"/>
    <dbReference type="GeneID" id="30200633"/>
    <dbReference type="OrthoDB" id="17927at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21673">
        <property type="entry name" value="SMP_Mdm34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03105">
        <property type="entry name" value="Mdm34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027536">
        <property type="entry name" value="MDM34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058825">
        <property type="entry name" value="MDM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28185">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28185:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26545">
        <property type="entry name" value="Mdm34_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="12">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="334"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="463" mass="52817" checksum="F0AF7A48DEF6C235" modified="2017-01-18" version="1">MSCNISWDSFEKGSFLSWSKELLYDALNSGKRPHILSSEIKIKELTFGKQSPSFEVLEVGDLANDRFRGIFKLKYDGDANITLNTSIQANLLNIYEKSTQEFQGQFALPNFKLASQPFSLPLNVNLSNIKLSGIIVVVFSKSKGLTLVFKNDPLENIKVSSTFDTVPVIEKFLQKQIENQIRDLFRDILPSVLHKVSQKWTTNNIISQLHSKINEQNNEGLLNKERVSIFEVNPSEPELSPANMLKLSTLTTSRQSFRLSIPPLHDVIERPSLYKFELKSSLGKNLELGTKKIPIEIFSKNHDDFQIKNTLNTISRIQSKYYSNEKNNHKLKRRSIKMNSKKTKNPTPPRSVSMNTEATLVNDDQEDEIQLKEQPIIIDSKKNYDIKNNYHLDSAPLLTPSPINLTNPLLNRPPLTPIHTHHHSSADIFHYRSHSPRKSQSYLLNVGIGINNYGFLEAPPPYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P521</accession>
    <name>A0A1E3P521_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_30381</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039541.1">
        <property type="nucleotide sequence ID" value="XM_019181729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P521"/>
    <dbReference type="STRING" id="683960.A0A1E3P521"/>
    <dbReference type="GeneID" id="30198975"/>
    <dbReference type="OrthoDB" id="2985014at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17327">
        <property type="entry name" value="MFS_FEN2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000068">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000018">
        <property type="entry name" value="MFS transporter permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF24">
        <property type="entry name" value="NICOTINIC ACID PLASMA MEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="363"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="429"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="44"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="482" mass="53832" checksum="1902F940EA3B87E9" modified="2017-01-18" version="1">MTEVKSASENSFVNGNVKELGEFDDLSIDPVKEAKLVRKLDRFILPMFCVIYFLSFLDRSNIGNAKIVGLDDQLGLTQSQYSTAVSVFYATYICVEIPGVLLVKRLGPHRFITIALVCWSLVTIFTCFVRSYGTLVLTRLLLGLFEGSVFPSQSLYITMTYKRQEQAKRLGWLYVCSCASGAFGGLIATGITKIKPRGDFLSWSWLYVIEGCISLCAGVWVWFGLPDDPTKAKFLTEEEKHMMSIRAEQQRRYMGNQKFDKKEVIKAFIDPKVAISCLMQFSVDLVLYGFSTFLPSILKLQLGYDTMAAQYLSVPVYAVAAISVGSVSFISDRYNIKWPLILGLNTLGMIGYIILLTSKKGGVNYFATYLIAFPLYGSVGLSITWLNNNMAPHYRRATALGCNQTIGNLAGVVAGQIYRKSPYKLGNSFSLGCSVMGMICSVVMVKYLHYENSSKQKIMDGDKVDKKVERTGSDALDFKYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5F0</accession>
    <name>A0A1E3P5F0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inosine/uridine-preferring nucleoside hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_62695</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IUNH family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039339.1">
        <property type="nucleotide sequence ID" value="XM_019183420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5F0"/>
    <dbReference type="STRING" id="683960.A0A1E3P5F0"/>
    <dbReference type="GeneID" id="30200666"/>
    <dbReference type="OrthoDB" id="432381at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070635">
        <property type="term" value="F:nicotinamide riboside hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070636">
        <property type="term" value="F:nicotinic acid riboside hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008477">
        <property type="term" value="F:purine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045437">
        <property type="term" value="F:uridine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006216">
        <property type="term" value="P:cytidine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034355">
        <property type="term" value="P:NAD+ biosynthetic process via the salvage pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019358">
        <property type="term" value="P:nicotinate nucleotide salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006152">
        <property type="term" value="P:purine nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008655">
        <property type="term" value="P:pyrimidine-containing compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006218">
        <property type="term" value="P:uridine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02651">
        <property type="entry name" value="nuc_hydro_IU_UC_XIUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="entry name" value="Ribonucleoside hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023186">
        <property type="entry name" value="IUNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304">
        <property type="entry name" value="INOSINE-URIDINE PREFERRING NUCLEOSIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304:SF4">
        <property type="entry name" value="URIDINE NUCLEOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="Nucleoside hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="4">
        <location>
            <begin position="8"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="333" mass="37499" checksum="F7F921E4A09D0210" modified="2017-01-18" version="1">MTVTQIPIWLDCDPGHDDMIAILLSCFHPNFKLLGISTTFGNSSCENTTNNTLSILTKFQKTGIPVYQGWKVPLIKKPSFAPSIHGKSGLAGTELPTPQTSIENPSRDDKEQIEQFYSYLKTQVEKYENEISIVATGPLTNIGNFFQKYPDLKEKVKFVSIMGGGIEISNWKAGEFNIWCDPHSANFIFQDPIIAPKTILLPIDVTHTAILSESVEKRILNEPNTTPLRKMLYELMVFFKDTYVKEQGFVDGPPIHDPLAVIVLLEEYGVENMGIESIRYQLDTVEEGELEGKLEYKPNDRGVKVVTKLDIEKFWEIILKVITIADGDHAVEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5F2</accession>
    <name>A0A1E3P5F2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LSM complex subunit LSm2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_61129</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of LSm protein complexes, which are involved in RNA processing and may function in a chaperone-like manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039765.1">
        <property type="nucleotide sequence ID" value="XM_019183113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5F2"/>
    <dbReference type="STRING" id="683960.A0A1E3P5F2"/>
    <dbReference type="GeneID" id="30200359"/>
    <dbReference type="OrthoDB" id="10256176at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005732">
        <property type="term" value="C:sno(s)RNA-containing ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01725">
        <property type="entry name" value="LSm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000053">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016654">
        <property type="entry name" value="U6_snRNA_Lsm2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13829">
        <property type="entry name" value="SNRNP CORE PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13829:SF2">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016394">
        <property type="entry name" value="U6_snRNA_Lsm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="2"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR016394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="101" mass="11382" checksum="FD68938700FB0B04" modified="2017-01-18" version="1">MLFLSFFKTLVDREVTVELKNGIELKGTLKTADQYFNLKLDNVSTTNELKFPHLSSVKSIFLRGSTVRYVYISKNDVDTNLLQDAARREALANSGKPIAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5F3</accession>
    <name>A0A1E3P5F3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="9">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_22363</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">ATP-dependent DNA helicase involved in DNA damage repair by homologous recombination and in genome maintenance. Capable of unwinding D-loops. Plays a role in limiting crossover recombinants during mitotic DNA double-strand break (DSB) repair. Component of a FANCM-MHF complex which promotes gene conversion at blocked replication forks, probably by reversal of the stalled fork.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with the MHF histone-fold complex to form the FANCM-MHF complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the DEAD box helicase family. DEAH subfamily. FANCM sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="9"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039906.1">
        <property type="nucleotide sequence ID" value="XM_019180941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5F3"/>
    <dbReference type="STRING" id="683960.A0A1E3P5F3"/>
    <dbReference type="GeneID" id="30198187"/>
    <dbReference type="OrthoDB" id="164902at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033677">
        <property type="term" value="F:DNA/RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070336">
        <property type="term" value="F:flap-structured DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033567">
        <property type="term" value="P:DNA replication, Okazaki fragment processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007535">
        <property type="term" value="P:donor selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045003">
        <property type="term" value="P:double-strand break repair via synthesis-dependent strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060543">
        <property type="term" value="P:negative regulation of strand invasion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18033">
        <property type="entry name" value="DEXDc_FANCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12091">
        <property type="entry name" value="FANCM_ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000861">
        <property type="entry name" value="Fanconi anemia, complementation group M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1320.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039686">
        <property type="entry name" value="FANCM/Mph1-like_ID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044749">
        <property type="entry name" value="FANCM_DEXDc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025">
        <property type="entry name" value="FANCONI ANEMIA GROUP M FANCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025:SF20">
        <property type="entry name" value="FANCONI ANEMIA GROUP M PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="85"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="425"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="482"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="489"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="502"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="529"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ60699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="728" mass="83449" checksum="34AE8F160C1412AE" modified="2017-01-18" version="1" fragment="single">DFDDELEQALNKPAVNRQVQETVIRRNEPAQRTLMGARVENEPAQYEEIRREITYGPTHHKTNALDMNTFIYPSNLDMRDYQYDIIVKSFYKNLLCAIPTGMGKTFIASTVMLNYYRWFKEGKIIFMAPTRPLVAQQIQACLGVTDIPSSDTAILLDKTRKNRQVIWNDKRVFFTTPQVVENDLKAGTLNPKEIVCLVIDEAHRARGNYAYTNVVKFIDRFNTSYRVLALTATPAADIEGVQEVVDNLHISQIEIRTEESIDIVKYMKRKETVRIKVGLNSEMEDIIELLSDAINPVLKQANEANIYDVTDPSKINAFLVMQKSQAVIKNPTLHEGIKWKYYFILQVIGYVGQMLRRLKIYGVRTFYSYYQNKCKEFRTKYSMGKSTNKTSAGFFYHDALKKIEVICDKVLADPSYVSHPKLEHMIDELMTFFQHKKNSRVIIFTELRESALEIVKCIDSVAGEECKAHIFIGQAKGKEGFDEEEFTRKHAPKGRGKQKKLERKEREREALEKKEQKKQLEAQQRAASRTGTSEEAQLQGMNQKTQKDLIKKFKKGEFNILVATSIGEEGLDIGEVDLIICYDSTSSPIKNIQRMGRTGRKNDGKVILLLASNEETKFDQAMEGYAFVQKQIAQDYLDMHKSDRIVPKEIQPKCVKQFIDIPEENLAIAKGEDEEQVIKYATQAMLGKTPKSKKTATKKTTKQGKILGFFMPDNVEVGFVKSSKLVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5G2</accession>
    <name>A0A1E3P5G2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_92173</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tyrosyl-DNA phosphodiesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039264.1">
        <property type="nucleotide sequence ID" value="XM_019186349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5G2"/>
    <dbReference type="GeneID" id="30203595"/>
    <dbReference type="OrthoDB" id="47785at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017005">
        <property type="term" value="F:3'-tyrosyl-DNA phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010347">
        <property type="entry name" value="Tdp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415:SF0">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06087">
        <property type="entry name" value="Tyr-DNA_phospho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="9">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="100" mass="11055" checksum="03B06E3BD475E2A4" modified="2017-01-18" version="1">MSKQAWGTTPTKDTIKSQRPISAVSNYETGVLISPTDYGSGKKLVPLEIGEERKLSDDEIPIILPFRLPPEQYKADDQPWCMKNACNLPDILGAIHLGTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5J7</accession>
    <name>A0A1E3P5J7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cell wall protein SED1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_67399</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039928.1">
        <property type="nucleotide sequence ID" value="XM_019184080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5J7"/>
    <dbReference type="STRING" id="683960.A0A1E3P5J7"/>
    <dbReference type="GeneID" id="30201326"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038843">
        <property type="entry name" value="Sed1/Spi1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35523">
        <property type="entry name" value="CELL WALL PROTEIN SED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35523:SF1">
        <property type="entry name" value="CELL WALL PROTEIN SED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133708" description="Cell wall protein SED1" evidence="2">
        <location>
            <begin position="17"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="26780" checksum="0442A75D918F93C8" modified="2017-01-18" version="1">MKFLTILSAFSSVALAQYLNTTSSAASSSEAVDVTTITKDTVVTEFTTYCPYPTTIVTNNKTITVDHATTLTITDCPCSIKTTITTSGSIPPEETTAAPSESGHEEPGHTTTISKDTVVTEFTTYCPEATTIVTNGKTYTATGATTLTITDCPCTVPTHTVVTNTPGTPGTPAAPTGTTAPSTAPSQGTTEGTSPAPAPSHGTSQAPSGTTTQAPAGSSTTLQTSATVPGSSSGPASVEIQSSNGAGSLQKAIGGSIAGLIAAVAYLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5P8</accession>
    <name>A0A1E3P5P8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">WICANDRAFT_28137</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039855.1">
        <property type="nucleotide sequence ID" value="XM_019181453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5P8"/>
    <dbReference type="STRING" id="683960.A0A1E3P5P8"/>
    <dbReference type="GeneID" id="30198699"/>
    <dbReference type="OrthoDB" id="63989at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990332">
        <property type="term" value="C:Ire1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990604">
        <property type="term" value="C:IRE1-TRAF2-ASK1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070059">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036498">
        <property type="term" value="P:IRE1-mediated unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09769">
        <property type="entry name" value="Luminal_IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10422">
        <property type="entry name" value="RNase_Ire1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13982">
        <property type="entry name" value="STKc_IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000077">
        <property type="entry name" value="Putative Serine/threonine-protein kinase/endoribonuclease IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000572">
        <property type="entry name" value="Serine/threonine-protein kinase/endoribonuclease IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.180:FF:000002">
        <property type="entry name" value="Serine/threonine-protein kinase/endoribonuclease IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.180">
        <property type="entry name" value="KEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045133">
        <property type="entry name" value="IRE1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010513">
        <property type="entry name" value="KEN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038357">
        <property type="entry name" value="KEN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_rpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954">
        <property type="entry name" value="IRE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954:SF6">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06479">
        <property type="entry name" value="Ribonuc_2-5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00580">
        <property type="entry name" value="PUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51392">
        <property type="entry name" value="KEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="481"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="632"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="domain" description="KEN" evidence="22">
        <location>
            <begin position="941"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="528"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="528"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="540"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="583"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="ODQ60648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1074" mass="121816" checksum="964E80A969D86D46" modified="2017-01-18" version="1">MHFSKRNLLFVVLPILSILFLLISPVWLSSLSNAKYRTVRTITEDTIAAKRDAKIQRHHDQTDDLIRSQAYRSINEFQLSNLLLASDIEGGLHALDRETGEIRWSLDVEQPLVQISSNNSNHTSNDHKDSLVWMVEPYGDGNLYFFNKEIGLNKLPVSISHLVLQSPFALANDDKIYTGSRKSSLYSINVQTGEVLSSYGSGCDVFENVCKDDPEVVLVGKTTYELTIHSKDNTYWNVTYSAWGQNNIDVDLAYQNTIPLDGTYIAPFHDNTLLAIDVNSKIAKWVSQLPNTVVNVFDILQDEKLSDAQLTDNFIIVPHPLKPPHGFQEDENLGVYLDQTKNGSWYAMSSDYYPSLVKSAPTSRYSLSERWRTSHILNTHDLFITAVVGVHLPNENPQTIPDSSRALIGYPTNFPQQYPQKPIHTPRSDDIDTYNPPSRSEWIGLPSSSHPVLNIDPSPSDQYEFFNTVFGKFIYRCLENLVMVLITLGIVFVFIKLNWIPSINVMLKKNGLELKELNKEEVKIATQESLPTPSVVQANHDAESKDEVNKESTEAKVKKEVKILEPPESEKISNDKNNENNGLKKRKRGSRGGKKNKKNLDNTGSEANEVIPKHSTTNPANGVVSPKINGLTLSDNVLGYGSYGTVVFKGSFQNRDVAVKRMLIDFYDVASHEINLLTESDDHPNVIRYFYSETSDKFLYIALELCSASLEDVIEKSQDHSDLIKIMNPVSVLQQIAKGLHHLHSLKIVHRDIKPQNILVAPPKKIKARSLKDQFTPVRILISDFGLCKRLEADESSFRATTQHAAGTSGWRAPELLVDESNSTYNSCSVSSEHSFNTSTNSIVEPLVFDSLSRRKLTRAIDIFSLGCVFFYILTNGGHPFGDRYLREGNVIKDEYSLDALDILPDNVSEAKDLISKMITRNPKLRPNTSQVLNHPYFWDNARKLEFLLKVSDRFEVEKRDPPSDLLMKLELVAPNVIGDDWCAKFDKSFLDNLGKYRKYHPERLMDLLRALRNKYHHFNDLPADLVTRMSPLPNGFYQFFNKKFPNLLMEIYHVVEENLSDDHVLETFFKGVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5V2</accession>
    <name>A0A1E3P5V2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_51558</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040026.1">
        <property type="nucleotide sequence ID" value="XM_019182671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5V2"/>
    <dbReference type="STRING" id="683960.A0A1E3P5V2"/>
    <dbReference type="GeneID" id="30199917"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23813">
        <property type="entry name" value="UBCc_UBE2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000020">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="3"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="150" mass="17049" checksum="36BD89D49EFCDECF" modified="2017-01-18" version="1">MAALPKRIIKETERLVSDPVPGITAVPHEENLRYFDVTIQGPDQSPYEGGVFKLELFLPDDYPMIAPKVRFLTKIYHPNIDKLGRICLDVLKNNWSPALQIRTILLSVQALLAAPNPNDPLANDVAEEWLENENRAIQTAKEWTKNYAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6I7</accession>
    <name>A0A1E3P6I7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_77586</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040127.1">
        <property type="nucleotide sequence ID" value="XM_019184576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6I7"/>
    <dbReference type="STRING" id="683960.A0A1E3P6I7"/>
    <dbReference type="GeneID" id="30201822"/>
    <dbReference type="OrthoDB" id="5550281at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008301">
        <property type="term" value="F:DNA binding, bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044804">
        <property type="term" value="P:nucleophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001034">
        <property type="term" value="P:positive regulation of double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070550">
        <property type="term" value="P:rDNA chromatin condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006356">
        <property type="term" value="P:regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001174">
        <property type="term" value="P:transcriptional start site selection at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF24">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="4">
        <location>
            <begin position="131"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="2">
        <location>
            <begin position="131"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="94"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="215"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="240"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="262"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="274" mass="30443" checksum="F62D4B12CE3B39C4" modified="2017-01-18" version="1">MSNQLKTAKDTLVSSLYELSKAANEASAATVDFYNASLTVEEDPNEVFKQLAESLRILTNASTGVSETARLIAASNSQIDPNVIQSSLINNNNNSLIPNQNNDHHATTTTSTPLTNEKPAKKKVEKDPNAPKKPLTVFFAYSAYVRQALREDRLAKGLPALSSTEITQEISKKWNELGEAEKEKWKEAYNEELEHYHVNKEKYLELKKIGEAPILTGPNPAPVPIPAYLQKNHHDKSSKKRDSESSEKKDKKKKSKKRKSNAPDSTSTDIQIGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6V7</accession>
    <name>A0A1E3P6V7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_28970</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040237.1">
        <property type="nucleotide sequence ID" value="XM_019181564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6V7"/>
    <dbReference type="STRING" id="683960.A0A1E3P6V7"/>
    <dbReference type="GeneID" id="30198810"/>
    <dbReference type="OrthoDB" id="276546at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02933">
        <property type="entry name" value="OYE_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000138">
        <property type="entry name" value="NADPH dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF91">
        <property type="entry name" value="NADPH DEHYDROGENASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="4">
        <location>
            <begin position="16"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="393" mass="44007" checksum="73407B75D9729ABF" modified="2017-01-18" version="1">MPLIQDFKPIPLKDTDLFKPIKLGNHQLNHRVALAPLTRMRNVKNVPTDQMIDYYDQRSNQEGSFVISEATFISAKAGGYETAPGIFNEEQISQWSKIFKKIHENKSVVFQQLWQLGWQSIPSALAADGFKYVSASDEPYMDEASEKAAKECNNPQHGLTIPEIKEYVKDFVQASKNSLKAGADGVEIHSANGYLLNQFLDSNSNRRTDEYGGSIENRARFTLEVVDAIVDAIGANKVGIRLSPYGVFGSMNGSKDPEFLAVYAYVIAQLEKRAFDGKRLAYIHLVEPRVTSPLNPEGVGEVDGSNDFIYNIWKGVVIRAGDYALHPDIAAKDVGKPNTMIAYGRLFIANPDLPYRLKEGLALNAYNRDTFYAPTRVGYTDYPTYEEIKQEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6X4</accession>
    <name>A0A1E3P6X4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_103907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039919.1">
        <property type="nucleotide sequence ID" value="XM_019180392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6X4"/>
    <dbReference type="GeneID" id="30197638"/>
    <dbReference type="OrthoDB" id="10478783at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="301" mass="34182" checksum="21C78BF15E6DB796" modified="2017-01-18" version="1">MNSMKRNGLRVFVDQNSKTEESSSDDGDERGTPTQLKEAANIEEDINDDLNSPVFFEDSNSSSHSRTQSLEKVITMTSLHRTRTSSYNNKTTTPTPSIFNTPKADLHHDYTHTPSSSIVKLESGLVTPVRERLLEEDDMEHNRLEYSPTITIDERFDVKVDIPSEKALPRTTEDKIKFFETFQKKEAQEFDKTLKSLKKGGWITSKDLSSLETKKHNHFKKWEIKIKELKNSLRHKKLQQGIHGTPPISSAASGPVTPGGHIDDFSYFSPKIKNDVENFEGDRSPNLQTLEQQFSVLGISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P708</accession>
    <name>A0A1E3P708_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_61720</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040360.1">
        <property type="nucleotide sequence ID" value="XM_019183235.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P708"/>
    <dbReference type="STRING" id="683960.A0A1E3P708"/>
    <dbReference type="GeneID" id="30200481"/>
    <dbReference type="OrthoDB" id="5876363at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032221">
        <property type="term" value="C:Rpd3S complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15535">
        <property type="entry name" value="PHD1_Rco1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15534">
        <property type="entry name" value="PHD2_PHF12_Rco1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052819">
        <property type="entry name" value="Chromatin_regulatory_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47636">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN RCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47636:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN RCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="276"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="116"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="179"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="672" mass="75785" checksum="7A063A997103E263" modified="2017-01-18" version="1">MPHFPPLYPKSSVKSSRKGSPLPDESNGNRRRSNRLSELEQKDAVEKLKKSRNNHEADDPELVLKKRKFNIDGKTNLSKEGSTTTPKTSNRSVTLTLKGGQLQFNEPELGDPIISVTGLPLDAPPNSKIKKESLWPRKKTKKSKSTDAEDSEEDPNSTPEVSLPPENIEKQLRQGLKTNEYEKKLQPSNDRSRNVKPVPKKGAEIHGRKFVKSKIANVTTPKKKESKLKEGSKKNIKNASPKKTRENPFGFDNPMQLAGQHRENAFDFNDNTKDNEDFCSSCGEPGVFLCCESCPKSFHFACCDPPLSEDNLPDGNWFCNECKAKKNPPEPNPPGLFSKLLNQVDTRNPIQFKLPKKIRDRFEGVITGKYGEYEDNDYKPYRPTKIGAFEQTDPDLHFDKDGNILLCQRCGESGISPNKLNGEADKLITTCDYCPSAWHLDCLDPPLASVKQLGKKWKCPNHADHLLPNKPRRLRKQTIIDVDQVHGFKNDGNIEVKLRESDNEEDDEDEIHEIPTPAFLGNRDTQNGVAAKISKNTSKLWNDSYAIYRVPEEGVISDFLSKVIDLKEKEKNARVSELLNLTRENNVLLKRLSDGDEFNQDEKKAVLSLVELPELNFKELADIASKELDSLAFDDSTQSDEKISEAEVQELLSIKKLMALKGKDNLLKFLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P784</accession>
    <name>A0A1E3P784_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Threonine synthase</fullName>
            <ecNumber evidence="5">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_28579</name>
        <name evidence="15" type="ORF">WICANDRAFT_29453</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
            <strain evidence="15">NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-homoserine + H2O = L-threonine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10840"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.2.3.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:10841"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the threonine synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="5"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040199.1">
        <property type="nucleotide sequence ID" value="XM_019181508.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040291.1">
        <property type="nucleotide sequence ID" value="XM_019181606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P784"/>
    <dbReference type="STRING" id="683960.A0A1E3P784"/>
    <dbReference type="GeneID" id="30198754"/>
    <dbReference type="GeneID" id="30198852"/>
    <dbReference type="OrthoDB" id="5203861at2759"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00065"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01560">
        <property type="entry name" value="Thr-synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000046">
        <property type="entry name" value="THR4p Threonine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1380.10:FF:000003">
        <property type="entry name" value="THR4p Threonine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1380.10">
        <property type="entry name" value="Threonine synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029144">
        <property type="entry name" value="Thr_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037158">
        <property type="entry name" value="Thr_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051166">
        <property type="entry name" value="Threonine_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42690">
        <property type="entry name" value="THREONINE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42690:SF1">
        <property type="entry name" value="THREONINE SYNTHASE-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24857">
        <property type="entry name" value="THR4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14821">
        <property type="entry name" value="Thr_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Threonine synthase N-terminal" evidence="13">
        <location>
            <begin position="5"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR604450-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ60992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ61084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="510" mass="56793" checksum="23291FDC284F5BD2" modified="2017-01-18" version="1">MSSQTYRSTRATGEQAFSFEETVLKGLASDGGLFVPSAIPQVDASTIEKWSTLSFQELAFEIMRLYIKESEIPDEDLKKLVHNSYSTFRHEEITPLNPIDEANGLYLLELFHGPTYAFKDVALQFVGNLFEYFLQRKNAGKTGADRDVLTVVGATSGDTGSAAIYGLRGKKDVSVFILYPTGRISPIQEAQMTSVLDSNVHTLSVKGTFDDCQDIVKQIFGDVEFNKSHHVGAVNSINWARILAQQTYYFYSYFQLAKKVGSDKKIRFVVPSGNFGDILAGYYAKEMGLPADRLIIATNENDILDRFLKSSKYEKGGDVKATLSPAMDILISSNFERLLWYLVRNNVTKSDEKAGETINNWFAELKQTGSFTVPQEVIAAARKDFDSERVDNQETIKAIKDLYTSNTTKYVLDPHSAVGVTASYRHIAQDKADNVAGVNYISLSTAHPAKFADAVNQALSSIEGYSFDKEVLPEELRQLSSKELKIKLIEKADVELVKSAIIEELQKEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7F5</accession>
    <name>A0A1E3P7F5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_77544</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040079.1">
        <property type="nucleotide sequence ID" value="XM_019184569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7F5"/>
    <dbReference type="STRING" id="683960.A0A1E3P7F5"/>
    <dbReference type="GeneID" id="30201815"/>
    <dbReference type="OrthoDB" id="3981143at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF21">
        <property type="entry name" value="CHROMATIN STRUCTURE-REMODELING COMPLEX PROTEIN RSC3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="12"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="54"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="216"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="291"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="228"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="258"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="293"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="945" mass="108866" checksum="54BC1170FBA9D588" modified="2017-01-18" version="1">MEPRKRNRKPVVCTVCRKRKIRCDRAKPACSACKKSNVSHLCTYEEPAWAAYENSSTNNNSSQHGSSANNTPAPRSPFTFKPTATATAQIQQHQQQELQFQQQQFQQHLLQQQQYQQTLQNQIAAKYGSNGKPLTGAATAAPSSSCSTAGGAPISAPYYPYGPPDRRNPAPQYYGAEPSTNNNVLLPPIANQTYNNNPNLMSELEFLKNRIVQIEGYMGDHPPPPPHHQQQQGSQHAPNQYYTAPQQQLPHYNPPQPQQHQAPPSQTQATTPYGIQLPSINQIKPLFDPAKDSQLQESKSQDEQPQSDSTTKPNDSNIDPTLIDESEIITSFKTNTPNSKYLNMESSGIFETVSYAKKDDFQTNFYLCLQAHEEKLKAQSEIEESNTPLRKRVKLNDHFHNLLQNPKYQVETFFKYEATNLTEIIKKINKLLPYKKIIWLLVEKFFTSFADPFIPVLNEEEFSLAITKIIGEKELNDSKIKITADVNNSQDLVNLTYLLMILRISTVSLTNTIKLDKFNNFLLKNAPVSPEFDFIIEIMLIFLQNFSIDKMTRLKLKLLELFLFNKSAESSDVSDFYPAGLAISLNLNNKSEIASNNDQLIKIWYHLYFQYYNLNMVKGIPIFLNDEFFNLELSNFTNGNLGENFHKSIINIYGKQIELNKIIFKIWNKLYNFLNQEVTVGGLEHLIDAYDQFVLSNFKDYDTYKKEVNSNYSNKCFNLINNANHKLTKMNLNCFILIHYESEKTKDKAKFIKLLTGIFDLCIESVEKSFDVFKNYNSHFNAGYEFIVFPSLIALVDKNSKLLMATALRLWWANKPSLAIFKFLVNMVDELKGFTFLDNSFTCWKIKMKTLLFINLSKDAYLKKEDAMPLLVKKLNLENVEQDIKFDNLYTNFQDFTDTEMETFEKSLDRLNSKFDNKYSSMTTLDEFFNNLEIERPAFDIKLFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7G2</accession>
    <name>A0A1E3P7G2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_77987</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040562.1">
        <property type="nucleotide sequence ID" value="XM_019184647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7G2"/>
    <dbReference type="STRING" id="683960.A0A1E3P7G2"/>
    <dbReference type="GeneID" id="30201893"/>
    <dbReference type="OrthoDB" id="4081967at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035189">
        <property type="entry name" value="Std1/Mth1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17235">
        <property type="entry name" value="STD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="451" mass="51075" checksum="42FFC433606FDEF9" modified="2017-01-18" version="1">MYVSPVLSKTKSDTPKEYTNRARDEITRKLNLKPPSSQLRHGSSPLKNVSSASDHSLSKTNSYDSLEIPLTLSEKETTSKQENKPKSKLFGLSKLRHLNSSSSLKSSKKSSSEEDLIPSEPVDNDVQMEDYEYQQQLQPLHPVPSISSSFSSSASSISTTPSMTSKITNQDSIFSNQTLQTQYTNISQFSHPESIQEQHQQPGQPIMMTLQDALPSRFDDMYAPELLADPSLLIDGRPMFTKRALLDWELNDIRSLLIVDSIKPEWNNSLPIIYAPQGFKFEYLPLDADDETIVEKLVHSDIYKEAKFDVEFREQTAKYTLNAARQRHAMFLTNSGIPIFYNNRLSKPEWRNVIENFLLNLAVEAQCRYDFKKSCHELKKLKKAGNMSSNGNVNVSSSNGNSLLRKAIMNDSRTVVPGLPKLSKEDKAKLWTQVQAKVYKRIGLDWTPDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7Q7</accession>
    <name>A0A1E3P7Q7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Actin-related protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_90748</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040547.1">
        <property type="nucleotide sequence ID" value="XM_019186256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7Q7"/>
    <dbReference type="STRING" id="683960.A0A1E3P7Q7"/>
    <dbReference type="GeneID" id="30203502"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="276"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="309"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="430" mass="48989" checksum="3E6F272C3416A9E8" modified="2017-01-18" version="1">MEDVADDDTSLRPKIPKKRSFTHYSENSINFPKPNAELKPILEDGIITDWDSAVEQWEYLFDNLRADYKEQPLLLTEPVWNTKENRTKSLEIALEQFEFPGFYLASVPTCVSFALGRPSALVVDIGHDITSVTPVVDGMCLAKTTLKTHYAGKYLSKQLKLHIDSLDKEVIPLYKVKSKVLTQPNEKPNWVPKQFENITKSYEDYQIEKTLQQLKETIIKVPQDPITKDTKYDDEDEVFPKRSFELPNAHTIEFGKSRFEFGDSLFEPANYQIDGFEPESGEPEIKSNIDYVPLKRAKKPTEDDEQEEQEKTSTRGKKEVPRFRGLSELTSHALSLVDVDIRAQLAHNIIVTGGSSLIPGLNDRLLADLNKNHPGLKIRLHAAGNTAERKFQSWIGGSVLSSLGTFHQLWVSKKELEEVGVEKLLSERFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P889</accession>
    <name>A0A1E3P889_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_83629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040727.1">
        <property type="nucleotide sequence ID" value="XM_019185572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P889"/>
    <dbReference type="GeneID" id="30202818"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="95" mass="11000" checksum="A4C712FC22E95F43" modified="2017-01-18" version="1">MKRQLRKERKERERREMMARSGDVDQGAISQDNTNFDSLDDEDGETPIGGDEVDDVDINDLRYDIDNPAIEQYVLSGNSNDINVYDENINFEEQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8L7</accession>
    <name>A0A1E3P8L7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RNI-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_25043</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040960.1">
        <property type="nucleotide sequence ID" value="XM_019181091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8L7"/>
    <dbReference type="STRING" id="683960.A0A1E3P8L7"/>
    <dbReference type="GeneID" id="30198337"/>
    <dbReference type="OrthoDB" id="8436363at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052410">
        <property type="entry name" value="DRC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24107:SF30">
        <property type="entry name" value="GLC7-INTERACTING PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24107">
        <property type="entry name" value="YNEIN REGULATORY COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="844" mass="96118" checksum="D995D3750805ECDD" modified="2017-01-18" version="1">MSPTSGLLLGSLHQSPSQSHQQERVILNKNPNRSQLPIKNLSNIRLRRVTFALDKLPDDPPQQIPSRRPKKGNVIVVEDLVAGPSKLSVGITNESQIEAKQYDEKDLKAVMENQRRALEEAEKHAQEAHFAARRIANEVKNYKHSKKNEKVQEDELDVVADDVGNLEIDKPIHLHEHHYEEEDHETDEDIPLELIYTRCCHLREILPIPATLKQLKNKHSPLTTLKMLNPKPTLIDVYSFSDFIAIVPIKNLVFDNVSMNSEMLKVILSSLINSTTIEKLSLKNVPIDEIGWKYLCKFLSRNKSISKLDISQMKVKSDLPLSQHRSEMDWSLFINTLAHRGGLVELILNGCKLSTLDFCRLIDEGLSISTKRLGLVSMDLNFDQLKKLASWITSEYSTCEGVDLGFNDLSIDNNLKPLIKKLANNKNPRLAHMSFNSTGLTNVEDAALLIRSLSKLTNLNFLDLSNLKQIFPGIMPYLNKYLPRFPNLQRLHLDSNELSTKSFSILSGIIPKCVHLIHLSIMNQTAISYAAAATLYSAVKSSKTMLNLDIDYDLIDEKINSRIAVCLMRNMERTMGDDSHHHLDSYDDILFDGSLIAESAGKLLEKFNTNEIENDEFSKKFLKKKFLNKIMESRSNINKTIDELLTKQENKLLSLQEKEDLLRFYFLDNSLLKILEIFQNLNDEHSQQSDVQPHQMVTELNEGKETPIDVATGKPILLRSVSQTSIQAKKQEEEEGEFHRWGFFVQQQLPPPNQQDLATWAKLPSGEELRDAVIKAKGINSISDLIDNVNEHRFNLDKIYPTIKEQSSSATSENISDESADEYQEVDEVYDKLLNSLQKVRSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8N0</accession>
    <name>A0A1E3P8N0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DUF1682-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_77870</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040434.1">
        <property type="nucleotide sequence ID" value="XM_019184626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8N0"/>
    <dbReference type="STRING" id="683960.A0A1E3P8N0"/>
    <dbReference type="GeneID" id="30201872"/>
    <dbReference type="OrthoDB" id="10039147at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032469">
        <property type="term" value="P:endoplasmic reticulum calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012879">
        <property type="entry name" value="CCDC47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12883">
        <property type="entry name" value="ADIPOCYTE-SPECIFIC PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12883:SF0">
        <property type="entry name" value="PAT COMPLEX SUBUNIT CCDC47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07946">
        <property type="entry name" value="CCDC47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="321"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="321"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="357"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="42577" checksum="0F900AC9F38046AA" modified="2017-01-18" version="1">MSGIGAQAIDYINKLQSHYYSTDYEKLVELGFLGRVKLYDWHLEFAALGIIAVLVLFYQLGSFQNKSIVDTWIKGVRPVLDENFYQVGVKEDQLLIKDGAQLYTTYASGRINISGLLINFGLISRQNYFVYVFESLLSNFFESVKPQQDIVEIKIKPFPNERINNFIFAVVNKDGMNETRNDNYYLSLTKTSESTSLPREFVFMSESNELNESLLTEEFKTVLSKAGKVLKYFAITDLPQTKPVSESEYKSAPHLVLKLSLKTDRQSVETSKALIEQLIKFSDLITKLQLKHDSAKKINGVRNTELAKIKKAIEEQKLEELKEQKLEQERNARRDLAKLSPEEQAKFDKKQREKRERRQRNRQKQRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8Q0</accession>
    <name>A0A1E3P8Q0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_75947</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040955.1">
        <property type="nucleotide sequence ID" value="XM_019184294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8Q0"/>
    <dbReference type="STRING" id="683960.A0A1E3P8Q0"/>
    <dbReference type="GeneID" id="30201540"/>
    <dbReference type="OrthoDB" id="29851at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF32">
        <property type="entry name" value="HEAT SHOCK PROTEIN PDR13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="416" mass="46476" checksum="16D93ADB88CE1B5A" modified="2017-01-18" version="1">MSLTPIGIDIGTYKTVVSVVQNGGIDVILNNYSNRSTPTSITFDTKDIRFFGEDSKNQSVQNFENTITCFKQFLNNSNLAKEQEFISCKLAHPKHHQLGIVKVNDRSYEAVQVYAMFLGYLKQQIINNGVNIGKCVISVPGSYTPHERELVLIAASLVGLGDVDLCSEVLASGLTYAVRNHEGDVAKKHVIVDVGYSQMTISLLKIGERKVEVEKSFIDDSIGGRVIDKVICDQLLQKVGLKFDVHSKPYQRVLKECEKFKKVLSANNIVSVNIESIKEDKDIITSIKREEFEEWINESLVKVGSNIKKFLHSKEGPLKFESVQLIGGSSRIPSIKNLISQGFKKELSTTLNQDEAISLGCAYLSSVFTKKLDKSHFELKDFNHHTITISIGDNPTHYDLFHSFITSPSKEKVSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8X9</accession>
    <name>A0A1E3P8X9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="4">PPIase</shortName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_67915</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040534.1">
        <property type="nucleotide sequence ID" value="XM_019184106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8X9"/>
    <dbReference type="STRING" id="683960.A0A1E3P8X9"/>
    <dbReference type="GeneID" id="30201352"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000001">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009027342" description="Peptidyl-prolyl cis-trans isomerase" evidence="4">
        <location>
            <begin position="24"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="5">
        <location>
            <begin position="33"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="223" mass="24672" checksum="8164A7FDDB226485" modified="2017-01-18" version="1">MRLFSSAFTIVATFTLFISAVLAADPPITHKVYFDIKQGDEELGRVIFGLYGSVVPKTVENFRKLTISSDPEFGYINSIFHRVIPDFMIQGGDFTDRNGRGGKSIYGARFKDENFHIKHDKKGRLSMANAGKDTNGSQFFITTVLTPWLDGKHVVFGEVLEGFDIISKIEKTKTNWSNKPDVDIVISGTGELNEDGEVITSATLEESKATEAAKESAIVHDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P912</accession>
    <name>A0A1E3P912_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_102593</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040911.1">
        <property type="nucleotide sequence ID" value="XM_019180360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P912"/>
    <dbReference type="STRING" id="683960.A0A1E3P912"/>
    <dbReference type="GeneID" id="30197606"/>
    <dbReference type="OrthoDB" id="1747252at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="50S ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_uL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL1M"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00687">
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    </dbReference>
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        <property type="entry name" value="Ribosomal protein L1"/>
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    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
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    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="35"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="30898" checksum="BF2FC0DD14332BCC" modified="2017-01-18" version="1">MLSSNFFRKNVIRISSPIRAFSSSSIARADEYARSAEQKQRAQERELTKKNEQRKLSRRRALAREPADKSPYYHDIHTALRYLRASEVGRSPSEAVITVTSTLIAERGNAKLAGSVNFPKPMKDSKTLFFTTDESQAAAAKNAGVTLVGGPELIEQIKQGELDLSFDRAFATPEIASQLAPIARQLGPKGLMPSAKRGTVVEDIASTISEVSGSIPFRQKGENLSIAVGRANFTDREIMLNIIAASRAFREAIQTQKTKKKALLGHTTITSTHGPGILIDFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P956</accession>
    <name>A0A1E3P956_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_76124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ61943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019184326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P956"/>
    <dbReference type="GeneID" id="30201572"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="322" mass="36270" checksum="5C80FE788F0D46D6" modified="2017-01-18" version="1">MAKRKATWITEGSPALKKRRVTSYNKYDSLKVKVESPRLIFPNEEDDADDMELLLRPDPNTSNQETFVDGGTEGLTEEDPIYLSSSDDDDDDEEDEDDEETPPLTSSATEPSDHESTLQNRESNSQKYNELPSSTTDPLKSSQGALQHSQSEVPQTVPLDFAEDDDFGVFDFGNSYNDFVPDSEPEITPNNAIEDKESMKAAQKNGVPGDGLEQDHQGEDSNNENEESTTDDHVAANGIEEPIDNEIEQQSFEDDDSDDSTIEIVDVRPIPPKISIERLEELIDRTRYKGLLKRVSARNLMRSRIQSEQMQKFIESIMRDTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9B5</accession>
    <name>A0A1E3P9B5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_87041</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041188.1">
        <property type="nucleotide sequence ID" value="XM_019186089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9B5"/>
    <dbReference type="STRING" id="683960.A0A1E3P9B5"/>
    <dbReference type="GeneID" id="30203335"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010255">
        <property type="term" value="P:glucose mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000115">
        <property type="entry name" value="High-affinity glucose transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF17">
        <property type="entry name" value="HEXOSE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="289"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="384"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="419"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="29"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ61981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="546" mass="59550" checksum="B20696A4BCA9CCE5" modified="2017-01-18" version="1">MGLADHHLVKKFYNAEEKSEGSTFMAVFVGLFAAFGGILFGYDTGTISGVLAMPYVLKHFPKDQPGVNGGEGVFSSSEHSLIVSILSAGTFFGALLAPYMNDTIGRRWTLIISCAVIFNIGVILQVAAHDINLLVAGRVIGGFGVGLVSSTVPLYQSETTPKWIRGAVVSCYQFAITIGLLLASCVNKGTHNRDDSGSYRIPLAIQFLWALILSIGMIFLPETPRFFVHKGKSDEAMKSLARLRKLPADHPQLVDELGEIVANYEFEMSQGSSSWADCFSLKNSQLKRIFTGFAIQAFQQLTGINFIFYFGTTFFKNSGIKNEFTIQLVTNIVNVVATIPGIFLVDILGRRSMLIGGAIGMCTSELLIAIIGVATKSDAANKCLIAFTCIFIAFFAATWGCDAWVVIGEIFPLRTRAKSIAISTASNWLWNFAIAYATPYLVDDAPGSARLQSKVFFIWGGCNFLCILFAFLFVYETKGLSLEAVDELYESVPNAWKSAGFIPTEHNYSRKVAGDEVFDERKATITNVEDAKGGILEQRSTASESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9P4</accession>
    <name>A0A1E3P9P4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">superoxide dismutase</fullName>
            <ecNumber evidence="4">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_21875</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="4"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041227.1">
        <property type="nucleotide sequence ID" value="XM_019180921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9P4"/>
    <dbReference type="STRING" id="683960.A0A1E3P9P4"/>
    <dbReference type="GeneID" id="30198167"/>
    <dbReference type="OrthoDB" id="159229at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000007">
        <property type="entry name" value="Cell surface Cu-only superoxide dismutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0843">Virulence</keyword>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="11">
        <location>
            <begin position="27"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="205" mass="22746" checksum="067435972442A788" modified="2017-01-18" version="1" fragment="single">KAKEITNNPTDVVLRADFPSAFSQNVVGIIQFYSVNGTAKVHVDVTGLPKNAGVFQYHIHENPVGEDGDCEATGKHFNPYGASPDCEAQKDDSLCQVGDLSGKHGLINTTCFETYFYDPYLSLDPSHPQYIGNKAINIHLENLDKLACANIKVSKEPEDLVLLDYEAEAEQVRQYEEVTGVKIEDRVEESDEVADYNEDGEDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9V9</accession>
    <name>A0A1E3P9V9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_18693</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040939.1">
        <property type="nucleotide sequence ID" value="XM_019180802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9V9"/>
    <dbReference type="STRING" id="683960.A0A1E3P9V9"/>
    <dbReference type="GeneID" id="30198048"/>
    <dbReference type="OrthoDB" id="165352at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.20">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494:SF1">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="433"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="466"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ61732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="488" mass="56184" checksum="DDFECE16C987D3CB" modified="2017-01-18" version="1" fragment="single">PIRKRKHRARKKNRKYSESAYSSTAAKKRISWEPGIDINTTDVVIGSIGSAITVVDYSEGRYRVERTNVYSNILNTIEVETDMKRPDWAQVRWINVNGLSWEAIAAIGEHYDLHRLAIEDMIDIPQRTKTDIYSGHLFGVLPLIKLVRTKKHEPKDFDSWWNRILKKMDLYNYMDEEQEKDDDEKFNDQHKEQTLGEKISESHEMRRLNETFYPANYSKRRLRILDDHRPLSYRDLFVGVEQCSFFMLKEENTVISFFESSGDDIEQAILGRISTDETILRTSGDPSVLLQSIIDAIVDIIYPVITAYRKRLSEFEVDILTNPDIKHTQSLHLMSGELSKLRRSLLPTTALINSLKDHSSKKSSSSSKTKLQSNELSASNFMSSAAALYLADVSDHTQMFTDEIEGMITSIENLVGLIFNTISTESNNAMQQLSLVTVIFLPLSFWTGYYGMNFQSFGDLKENVSYYWKIAIPFSVGLLILITWSFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA16</accession>
    <name>A0A1E3PA16_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_60328</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041466.1">
        <property type="nucleotide sequence ID" value="XM_019182971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA16"/>
    <dbReference type="GeneID" id="30200217"/>
    <dbReference type="OrthoDB" id="4079943at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="25314" checksum="8B87FC5E8F1B502F" modified="2017-01-18" version="1">MNSIPTEIKLNILSQAHPSQLNTIASASNQLEDLVKYIISKKLQSHLDQQQQLLISLYSPNSKESNYKYYYTVDYEIQQYTTTFNIGSFHHKENLLKPSTDLTEKQSVQEINHKQLNHLDLTLNEDQSMAKISFALLLKNTPINETSLRLNKESLSNSVQLENLAFQFSLQQGELEPEKGGYDFDINYNYALNFNNFVIDNVSLLKMIEKDDQALIIRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA20</accession>
    <name>A0A1E3PA20_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_66542</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family. PPIase D subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041460.1">
        <property type="nucleotide sequence ID" value="XM_019184047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA20"/>
    <dbReference type="STRING" id="683960.A0A1E3PA20"/>
    <dbReference type="GeneID" id="30201293"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000029">
        <property type="entry name" value="peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000009">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0697">Rotamase</keyword>
    <keyword evidence="8 11" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="13">
        <location>
            <begin position="93"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="389"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ62253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="452" mass="50337" checksum="A543CBDCD4525DC3" modified="2017-01-18" version="1">MAIEDTQPKRAEEDVEEDDDDDEPDEWDQRINKTGCADENLKLQLCHADTGDWRQCTKEKIHLHEETTDQPKQSQSLISIVSKMSTEERPRVFFDISIGGEPKGRAVFELFTDIVPKTAENFRALATGEKGVGNAGVPLSFKGSKFHRVIKDFMIQGGDFTAGNGTGGESIYGEKFEDENFTVLHEKPFLLSMANAGPNTNGSQFFVTTVPTPHLNGKHVVFGRLLSGKSVIRAVERTATDQGDKPLKDVEIVDCGELPSDYVIEPSVPKDDGTGDIYEEVLADNDNVDVNDPESVFKAINELKDIGTKLFKEKDLTKAFQKYSKAASYLEEYFPDDLSEENLDQLNKLKVSVNLNVALVGLKINKFDETVKAATTVLEQDKVDDKSKAKALYRRGSAYLSLKNEEKALKDLNEALKLSPSDGAIIKAINDVKKIEQDRIAREKKAFSKMFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA34</accession>
    <name>A0A1E3PA34_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_22299</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019180937.1"/>
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    <dbReference type="OrthoDB" id="422362at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:methylation"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="SET domain"/>
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        <property type="entry name" value="SET2_Histone-Lys_MeTrsfase"/>
    </dbReference>
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        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22884">
        <property type="entry name" value="SET DOMAIN PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="8">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="9">
        <location>
            <begin position="100"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ62160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="13397" checksum="7A2C8CD795A91AE2" modified="2017-01-18" version="1" fragment="single">LVVEYTGEVIDLDEVEHRLNTFYKDVENYYFLGLEGNYVIDAGQKGSVARFANHSCDPNSEMQKWYVNNEPRIGLFAKRPIEAGEEVTYDYNFEWFENAEPQKCYCGSKICRGFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA46</accession>
    <name>A0A1E3PA46_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL18 C-terminal eukaryotes domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_25295</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040964.1">
        <property type="nucleotide sequence ID" value="XM_019181125.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA46"/>
    <dbReference type="STRING" id="683960.A0A1E3PA46"/>
    <dbReference type="GeneID" id="30198371"/>
    <dbReference type="OrthoDB" id="1618453at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00432">
        <property type="entry name" value="Ribosomal_L18_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.100:FF:000002">
        <property type="entry name" value="60S ribosomal protein L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_A">
        <property type="entry name" value="Ribosomal_uL18_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005485">
        <property type="entry name" value="Rbsml_uL18_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057268">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025607">
        <property type="entry name" value="Ribosomal_uL18_C_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410">
        <property type="entry name" value="RIBOSOMAL PROTEIN L5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14204">
        <property type="entry name" value="Ribosomal_L18_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17144">
        <property type="entry name" value="Ribosomal_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00058">
        <property type="entry name" value="RIBOSOMALL5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL18 C-terminal eukaryotes" evidence="6">
        <location>
            <begin position="236"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="300" mass="34084" checksum="B396DE4A60F00B23" modified="2017-01-18" version="1">MPFQKSEKSSAYHSRFQTPFRRRAEGKTDYYQRKRLVAQHKAKYNTPKYRLVVRFSNKDIVAQIISSQITGDVVFTAAYAHELPRYGITHGLTNWAAAYAVGLLVARRALQKLGLDETYKGVEEVEGEYELTEAVEDAPRPFKVFLDVGLQRTTTGARVFGALKGASDGGLYIPHSENRFPGWDIEGEELDAETLRKYIFGGHVAEYMEELADDDEERFRGLFKGYLADDIEADGLEDIYTEAHEAIRADPSFKPTEKKFTKEQYAAESKKYRQVKLTREERAAKVAAKIAAFQAEQQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAH1</accession>
    <name>A0A1E3PAH1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_20312</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase type-B-like family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="3"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041598.1">
        <property type="nucleotide sequence ID" value="XM_019180864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAH1"/>
    <dbReference type="STRING" id="683960.A0A1E3PAH1"/>
    <dbReference type="GeneID" id="30198110"/>
    <dbReference type="OrthoDB" id="273917at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071044">
        <property type="term" value="P:histone mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071042">
        <property type="term" value="P:nuclear polyadenylation-dependent mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071036">
        <property type="term" value="P:nuclear polyadenylation-dependent snoRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071037">
        <property type="term" value="P:nuclear polyadenylation-dependent snRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000006">
        <property type="entry name" value="non-canonical poly(A) RNA polymerase PAPD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000003">
        <property type="entry name" value="non-canonical poly(A) RNA polymerase PAPD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002058">
        <property type="entry name" value="PAP_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF15">
        <property type="entry name" value="INACTIVE NON-CANONICAL POLY(A) RNA POLYMERASE PROTEIN TRF4-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03828">
        <property type="entry name" value="PAP_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="12">
        <location>
            <begin position="91"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="PAP-associated" evidence="11">
        <location>
            <begin position="282"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ62391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="413" mass="47108" checksum="DB35246B0BB9396B" modified="2017-01-18" version="1" fragment="single">SSIQITNERSVEPEKAKTGNDLDDNNDFISLNFSDSDDDEDEGQLDNEEDAYSMDENETDINLNSKVVKNDVPWIKNHDHSTQKEIADWLTLEIKDFIAYISPSKEEIELRNFTVRRLRESITKFWPDCEVHVFGSYATDLYLPGSDIDMVIVSDHKGYDSRNSLYSLSSYLKKERLATNVEVIAKAKVPIIKFTEYHSNIHIDVSFERTNGIDAARTIRSWLVETPGLREIVLIVKQFLSARKLNNVHVGGLGGYSIICLVYSFLQLHPRLSTGNISPYENLGVLLIEFFELYGKNFGYDNVAICVEPDNVCYLNKKYYPDLQGRSSFALAIQDPSDPQNNISRGSFNIRDIKKAFAGAYELLTNGCYDLESATYKQRIGKSILGNVVRYRGKERDFNDERSLVINEALHED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAI0</accession>
    <name>A0A1E3PAI0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dystroglycan-type cadherin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_76596</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041636.1">
        <property type="nucleotide sequence ID" value="XM_019184416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAI0"/>
    <dbReference type="STRING" id="683960.A0A1E3PAI0"/>
    <dbReference type="GeneID" id="30201662"/>
    <dbReference type="OrthoDB" id="41532at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006644">
        <property type="entry name" value="Cadg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05345">
        <property type="entry name" value="He_PIG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00736">
        <property type="entry name" value="CADG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133750" description="Dystroglycan-type cadherin-like domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="481"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Dystroglycan-type cadherin-like" evidence="4">
        <location>
            <begin position="27"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Dystroglycan-type cadherin-like" evidence="4">
        <location>
            <begin position="135"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Dystroglycan-type cadherin-like" evidence="4">
        <location>
            <begin position="336"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="513"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="749"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="812"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="843"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="427"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="545"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="756"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="812"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="846"/>
            <end position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="862" mass="93604" checksum="CE618971F0BC9BE9" modified="2017-01-18" version="1">MSFQLILRCCFILWFTSIIRAQPREGFPFDEQLPTVARANEAYEFQMSNETFISSDGDVTYSAKDLPSWLSFDPTSRTLSGTPNDQDVTDSAGFTLMGQDNDGEITKQYSIVVSKNKGPTPSDSYTVLSQLSDFGQTNGQNGLVLAPGKVFNVTFDSKTFDSDDEIKGYYGRSSDRSSLPNWLFFDSSTLRFSGVAPAVNSEIAPGFQYSFIFIATDYAGNAGSWVDFDIVVGYHDLTTSLKGVTNVNGSSGDDINFEIPLENVYQDGAEISLDNISYAGLNGAPDWLKLDNYTLVGTVPEDFKSGSNDTFNVTIYDHYSNSVVLYFAIETIDSLFSVDSFRDANATRGSYFQYYFLPSQFTDFSNTNLTVEFEDADWLSFHSSNLTINGETPDEFDETQVTVKAHLKNSDDSDELSFKIYGSDPLSSSSSSRSHSSTSSSSSRSSASASSSSSSGATVSATSASSNGNVSKSPSGRNKKALAIGLGVGIPLFLILVAGILIFLCCFKRRKNDKSDEEDSSNSPKISKPILGNPANGPNSRSPNGASPMSGAAAASGVLDEKQDWNEPHRLGALNVLKLDGKDLYENESNSSSLTKGDSFDSIHDDTHSSLYHDALQAQSTDMLMSGGAAAAGVGAAANHYNKDKDAIPKKSWRQTIDSKINRESLNSLATVSTNELFSIRLADDDDIRKDPRKSNLSFRDSAFLGSTASSILTRDDSGNIQKLDSDGNIVDLKNRHSKGRSLDVLKEEQTPHLHQPSSFQPTTNKSSLVNDTSFSSNATSSTGGEEFYPVETTNGIEWKQNQKNKFTFENGSVHTLGDTKTQGNNGNNNKARLVDFTNKARADTSNDVSQSTYETAEIESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAL9</accession>
    <name>A0A1E3PAL9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="13">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_76622</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PPP phosphatase family. PP-2B subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="13"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041662.1">
        <property type="nucleotide sequence ID" value="XM_019184421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAL9"/>
    <dbReference type="STRING" id="683960.A0A1E3PAL9"/>
    <dbReference type="GeneID" id="30201667"/>
    <dbReference type="OrthoDB" id="5593063at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033192">
        <property type="term" value="F:calmodulin-dependent protein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097720">
        <property type="term" value="P:calcineurin-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07416">
        <property type="entry name" value="MPP_PP2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041751">
        <property type="entry name" value="MPP_PP2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043360">
        <property type="entry name" value="PP2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45673">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2B CATALYTIC SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="14">
        <location>
            <begin position="178"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ62455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="578" mass="65616" checksum="BBF54D782C109FBF" modified="2017-01-18" version="1">MSSAQENSEKIDAALRALNKNPEVVVHSRDYSIHITEDGQKVSTKSRVNNNVPPIANFKPTNEQLFLPSGIPNIQFLKQHFLQEGRLTEDQTIRILKMASDVLSKEPNLLQVPAPVTVCGDIHGQYFDLIKLFEVGGDPKDTSYLFLGDYVDRGSFSIECLLYLYSIKITYPSTFFLLRGNHECKHLTDYFTFKMECLHKYSLNVYKAACDSFNTLPLAALMNNQYFCCHGGISPELNKPDDLNKIDRFREPPTRGLMCDILWSDPVEDYDENTDDDDALFIPNSVRGCSYAYTFKAVSNFLTRNNLLSVIRAHEAQDAGYRMYKRTETMGFPSLLTMFSAPNYLDTYKNKAAVLKYGNNVMNIRQFNYTPHPYWLPNFMDVFTWSLPFVGEKVTDILVSVLNICTEEELDEDTPLDKGTLQRFNEGVADVVGTASAPVAPVKRTTPPTDANDEVDELTLKKRALRNKILAIGRVSRMFQVLREEAENVEKLKQITGGVLPKGSLLGGSDGLRQQITSFEEAKKADLVNEKLPPTLDEVKLQDQKREEELIKQIDDSVHKDSKIQRLARRYSNSSFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAW0</accession>
    <name>A0A1E3PAW0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HotDog ACOT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_98915</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl coenzyme A hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041637.1">
        <property type="nucleotide sequence ID" value="XM_019186672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAW0"/>
    <dbReference type="STRING" id="683960.A0A1E3PAW0"/>
    <dbReference type="GeneID" id="30203918"/>
    <dbReference type="OrthoDB" id="331699at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03442">
        <property type="entry name" value="BFIT_BACH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000032">
        <property type="entry name" value="Acyl-CoA thioester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655:SF0">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="5">
        <location>
            <begin position="93"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="HotDog ACOT-type" evidence="5">
        <location>
            <begin position="302"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="505" mass="57129" checksum="E4AFC8B7F6791341" modified="2017-01-18" version="1">MIRYTKSLKPLQPLRALTPGSNDASAAEDASTTILTDKRDASTVVSTFDDATKRATWLTALAERKSQLAQGKVIDSFAYNTPQTPVISDKTRSESFSYLTLNFKEDIWLTDFYVNAAGRLRMGQIFQDLDALAGRISYRFVSPAAPIIVTAQVSRIYMLKRVDEIENKNFILSGSVVWTGRSSMEILIKASTTESSIKKDLKEEDIKDDDVFLTASFTFVARNPETHKSFPINRLLPITEKEWIDYRRAESQNAAKKLAKVNESLDKRPPTSEESQLIHKMFLTSKELANYNPLPKNVSFMKDTLTQSTSFMQPQYRNRHSYMIFGGYLMRQTFELAYCTAASFSCAFPRFVSLDSTTFKAPVPVGSVLFMNSTVVYTEHIHEEDVVPKDDLKAKIFDKDSFLHFDAVPTNKISRNPDDFLNRPGTLIQVKVDTSVKALNDPKQTPSGSFVYSFFVPKDSTEDSIPDPGFSSVIPETYSEMIEFIEGRRRALETARYAQQIKEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAW6</accession>
    <name>A0A1E3PAW6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SANT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_76735</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041773.1">
        <property type="nucleotide sequence ID" value="XM_019184442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAW6"/>
    <dbReference type="STRING" id="683960.A0A1E3PAW6"/>
    <dbReference type="GeneID" id="30201688"/>
    <dbReference type="OrthoDB" id="272624at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001156">
        <property type="term" value="F:TFIIIC-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070898">
        <property type="term" value="P:RNA polymerase III preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019328">
        <property type="entry name" value="PIGH-H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039467">
        <property type="entry name" value="TFIIIB_B''_Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22929">
        <property type="entry name" value="RNA POLYMERASE III TRANSCRIPTION INITIATION FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22929:SF0">
        <property type="entry name" value="TRANSCRIPTION FACTOR TFIIIB COMPONENT B'' HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15963">
        <property type="entry name" value="Myb_DNA-bind_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10181">
        <property type="entry name" value="PIG-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="4">
        <location>
            <begin position="709"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="169"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="274"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="610"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="791"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="217"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="386"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="396"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="454"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="484"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="610"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="635"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="881" mass="98728" checksum="276F9811CD2683DD" modified="2017-01-18" version="1">MNNSCPNLVKVCDNENQLKYVYHHNNKTPIILKALVLLITALLLSITPSFPYRIYSFLVIIGSIVIFNSIKINQRIEVKETSGIFHNSVQHKFISSEQVIDVVINEGAYGLQFIYYLAIIVKDLDRLVLIFPETDLRLAHLEKLRAEIRKKSHQYSRAMSSIVNKSGVRFAPKAKQRRSTTGTPIASRRPSVTPTPDTSRRSIDASFEDTNNRAGKEDDDENSDDDDEDDGPLLKPSTIEMPKPQETKQRRLSTLSNISNRDIFKKVSVQNGYTHDATSSSNADRKTHIIGIPQPPAPVKRRRSTVSQSRRGSALKRKSVSEVPGATPPASAAGTATPSAASSVPPPPILQKVSEKEAPVEKPQVSRPTTIETTSKDKGETDEPLSPTSSSQDPFTSSLPIPSTLPSSLPSTAQDGSKSQKSSETPVEQQSRPVSAPEASRPAVDNNASKEQSPMPIGIPIIRRPFTAPTLSKAEGKAPEDDTVASSPAPQAPSHTNDEDTGRMTEGLYFDEETQQLRKIMYDPKDKTPGFEILTSFQAKKYDQFSRNFSSINPELSQHITIDEESFTIQDLCKPTLPIGKVSSNFHQAQEARKSKMKQRKEKRELRRLAKENRVSLESLTDKDSVKKETKKEGEEGEGQDGEPEEEQKVASSSNAAIQLKVGADGNLIVDEDSRLIDRHVNIDNDTRERYNQNPFENVVNSASYGKQRYTDKWDENEVLKFYRALGQWGTDFGLIAQMFPHRTRRQVKAKFILEEKKRPRLVDLALNNKLNGNFDFESYCSDANKTFGTLNDFNTKLEQLKKDHEENLKELSIAREKAKEEDLIRQKKRELEVKSGQGTRQLTRQERLLELRKHETVLGSIDDIKKQREEEEAAAAAALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB65</accession>
    <name>A0A1E3PB65_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_88131</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041747.1">
        <property type="nucleotide sequence ID" value="XM_019186141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB65"/>
    <dbReference type="STRING" id="683960.A0A1E3PB65"/>
    <dbReference type="GeneID" id="30203387"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="210"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="219"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="243" mass="27756" checksum="23D9DB7A9A404EB2" modified="2017-01-18" version="1" fragment="single">MHSKGIVHRDLKLENLLLDKHKNIIITDFGFVNSFGPHNDLMKTSCGSPCYAAPELVISNEPYEARKVDVWSCGVILYAMLAGYLPFDDDPQNPDGDNIARLYQYITNTHLTFPEYVEPMPRDLLRRILVSNPRKRLTLKEVRGHQWLTPHAPFLSVTPAEWDKNYKTNNEHNANIVKTQRNLVKNDIRRYSLIDTTSSSFQNHNVSNHNFFANPLPPQTSSNHAIALPSSTSSTSINEQLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB98</accession>
    <name>A0A1E3PB98_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myotubularin phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_26024</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041364.1">
        <property type="nucleotide sequence ID" value="XM_019181217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB98"/>
    <dbReference type="STRING" id="683960.A0A1E3PB98"/>
    <dbReference type="GeneID" id="30198463"/>
    <dbReference type="OrthoDB" id="271628at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17666">
        <property type="entry name" value="PTP-MTM-like_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF128">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3,5-BISPHOSPHATE 3-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="5">
        <location>
            <begin position="165"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="593"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="649"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="604"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="670"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="693"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="2">
        <location>
            <position position="394"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="330"/>
            <end position="331"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="394"/>
            <end position="400"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="705" mass="81752" checksum="33F7BB4111B36EFD" modified="2017-01-18" version="1">MEYIKVARVDDVLLHKRGYIIHGSLHLTTHHLIFTVTNPRPNELRELWLCYPMISSVERKKGSALLNLIDINVDINDNEGNEIQSKIIKSLPKLFNQRGKELNLFKLSTLKINCKDFNFLSFDFENESKCFDVFESIMKLTCLGELNQLYAFIYQPNSIEKLLNTWKIYDPIKEFQRQGLIFDNDSQIVEQSSSNWRISNVNNNYNFSDTYPSTIVVPKTISDSVLTHASKYRSKQRIPALTYYYKKNGCSITRCSQPLLGLKQTRSIQDEKLVDEIFKSSSNNDNGNPKNLIVDARPTTNAMAQTALGAGSENMDNYPNCEKLYLGIDNIHVMRDSLNKLIDILKSGDLNKPPINKSSLKKTNWLKYISILLISTEKLVKSMILNNSNILIHCSDGWDRTSQISSLIQVCIDPYYRSIQGFITLIEKDWLSFGHRFNERSGHLSSESIFHDESNGSNNNQASLAFKSVSNHFKKKKHLKFTSPIFHQFLDSIYQLIIQFPNKFEFNERFLRRLIYHLYSCQYGNFLFDNEYERVTNKVFDKTRSVWDYFLSRKHEFTNKQYVEPTDQFDWLSPNYKSLVWWWQLYGRSDEEMNGDSHNEENVENGNSGLELQNSGLKSPEQQHPIIQPIPESAGTKVNNKEETLSKSKVFALTPPPPSIHKVNANYNNDDNDDDDDENENELKTRNGINDIRSPHKMMEKMSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBS2</accession>
    <name>A0A1E3PBS2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_26988</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042076.1">
        <property type="nucleotide sequence ID" value="XM_019181324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBS2"/>
    <dbReference type="STRING" id="683960.A0A1E3PBS2"/>
    <dbReference type="GeneID" id="30198570"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000111">
        <property type="entry name" value="GLI family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF120">
        <property type="entry name" value="KRUPPEL-LIKE FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="58"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="58"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="188" mass="20809" checksum="F3AB8534C2FB561B" modified="2017-01-18" version="1">MSSNHIASINNLINNDQEDEDRKFLRAAAEAIVATSMNNEIDPTILELLRRIQYANAPNSTTSPLATTTTTTPNISSNPLEPRTTNTPNDSTTPPSAKNINGHEPKPFICNDCSLSFRRSSDLRRHQRAHLPILPHICRMCGKGFARKDALKRHSDTLTCKRNREKLLSSGGDVESILAEAKKQGHDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBV5</accession>
    <name>A0A1E3PBV5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carbohydrate kinase PfkB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_82833</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042103.1">
        <property type="nucleotide sequence ID" value="XM_019185448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBV5"/>
    <dbReference type="STRING" id="683960.A0A1E3PBV5"/>
    <dbReference type="GeneID" id="30202694"/>
    <dbReference type="OrthoDB" id="497927at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47098">
        <property type="entry name" value="PROTEIN MAK32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47098:SF2">
        <property type="entry name" value="PROTEIN MAK32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="1">
        <location>
            <begin position="117"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="357" mass="40005" checksum="B9C9DFEFA363BB28" modified="2017-01-18" version="1">MTNDCIFTSMGMFIIDEIHFPENSSKRSHYNVIGGGGCYGALGSRIITGPSNSEKVGWIIDKGNDFPKDVEDYLKSWNTGAIWRETPERLTTRGWNMYGEREFREFKYLSPKLRITVDDLIENKPLLNSQSYHLICSPQRSIEILESLQKARSQSLPSPIITWEPVPDLCLPENLKDCLSVLPNLNVLTPNAEEAARFFSQPEPVTKQQHEELAAKFISNLTNDSQFQTGSGIVLRSGALGCFILTSKGINEWIPAYHNENNPTRSVIDPTGGGNTFIGGFTTGFVMSEGDWRIAALCGNIAAGIAIEQIGMPVFKVKKEGEDEWNGKSFKQRVEDYVEWNKLDFDVGEVLRVLKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBY0</accession>
    <name>A0A1E3PBY0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_77040</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042088.1">
        <property type="nucleotide sequence ID" value="XM_019184492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBY0"/>
    <dbReference type="STRING" id="683960.A0A1E3PBY0"/>
    <dbReference type="GeneID" id="30201738"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000134">
        <property type="entry name" value="MFS sugar transporter protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF24">
        <property type="entry name" value="HEXOSE TRANSPORTER PROTEIN (AFU_ORTHOLOGUE AFUA_8G04480)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="367"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="392"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="434"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="494"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="60"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="570" mass="63629" checksum="F3E3F0A081054B8C" modified="2017-01-18" version="1">MVGIIADEAKLFRKKRAKHKTSIGETDDSSDKIVSHLDEFIPNYGKLWIRVPHLVHLNFLMFIVSLTATNSGFDGSLLNAFQSMDDWKNAMGKPSGAVLGALNNGTVFGSLLAFFIASYLSDKFGRRHAISIGNVIMICGVIVQSCAGTWLNTTDQAIHNDKRCFAMSLVARIILGFGAMISGVGAPPLMSELAYPSHRQAVTAFFNSNWYLGSIISGWVSFGTRNLGNNWCWRIPTILQAFFPIIQLFLIYLVPESPRYLISKGHPEKAREIFVKYHAGGNEELCSGLIDFEMSEIQSAIEQEVIADKSSYKDFFRTPGNRKRLFLACFIGIIMQLSGNGLVSYYLNKVLNSIGVTSTTEQLIFNGGLQIYNYGISIIINLTLFQKGKRRIVFITSIAMMLLFYVIWTILSAINQKRNFEDKSLGKGVMAMIFLYYLSYNLGLNGLPYLYLTEISGFFLRTKVINTCQLIQQFASIYNGFVNSIAMDAIEWKYYIVYCCILAVELVVGYFTFVETGGLTLEEVAEVFGESIGDVDYNYIKEKNAIATGHIEEASPHEDSASTEKSANLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC37</accession>
    <name>A0A1E3PC37_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_60505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041654.1">
        <property type="nucleotide sequence ID" value="XM_019183009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC37"/>
    <dbReference type="GeneID" id="30200255"/>
    <dbReference type="OrthoDB" id="10676580at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="532"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="415"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="470"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="617" mass="69443" checksum="BED6C177577562CD" modified="2017-01-18" version="1">MEKEQSQDDFIRERLSHNFEAYTNNAILNKLTPSRSLGRASSVQSSSRKSDSSWGKKYPKRNDSNIVTSPVKTLRQFGAKDGGADDGADDDDDEYDTTTNYDDDNSKKDSTTVNVPLQSSPKKPLRKGTPKVPKTQKTKDAKSEKKKKSDTQHVPSTADLHNSSNRRIVTNSHINHHVSTQGIDDDGAGAGGAHLHSDNTLLSEIPSDILSPEKHIYPQENLRRSSSNIPSATKVKDATTSLLSKSFPVFSPPPPKHKESKTTGKSPTSGSKRRESPSKGNRGESLPPSLYPKVRLFGDEDDDEASAPKKLTSDVTDSKVVEEVRESLSIRKKRESEAITMFFDRSSSSSKAPERQSIISPRSKESNTRPNLFERTTHDNNSSFHTLNEFIKNPLIDDAKYKTTNVKHSDKKTPNNEPTETTNTTTQRKSFDISQFESSDEEPVPELTVPLNEEPQPTTTKRVYDEPIETSTNKKPKTTTTTSLYPTIQPTSQTPLKRSSESRRRSLYPTLPSVGHPSRVSAADIEDISNNIQETTKPKPQAPLPKEPEEELGDSDHWVKNQWACFFKAFKRYKKTNDLSIFQNKRLLEYLRCDEHEIKLKIQFTLDHEHEIKRKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCD6</accession>
    <name>A0A1E3PCD6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_27213</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042091.1">
        <property type="nucleotide sequence ID" value="XM_019181347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCD6"/>
    <dbReference type="STRING" id="683960.A0A1E3PCD6"/>
    <dbReference type="GeneID" id="30198593"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072655">
        <property type="term" value="P:establishment of protein localization to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000001">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133779" description="J domain-containing protein" evidence="7">
        <location>
            <begin position="21"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="23"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="9">
        <location>
            <begin position="145"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="6">
        <location>
            <begin position="145"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ62884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="41229" checksum="9780CF4BFEBDEFD6" modified="2017-01-18" version="1">MQFSSLVFLICAALITTVLCEADYYKILGVDKKASEKEIKSAYRALSKKFHPDKNPGDEDAHHKFIEVGEAYEVLSDDEKRSTYDRYGHDGLKNGGRPNGGGGGFDPFADFFGFGRQNHQQQRGKPRGHDADVNVDLSLKDFYTGTDFKFDVEMQNLCSKCEGTGSSDGKTHQCGGCNGSGVKVMKRQLAPGMFQQFQTTCDECGGKGKIITHKCKHCGGQAVTRGVRHFEFYVEPGTARDFTHKFKGDGDHSPDWEAGDLLVHLKERSHTNSGYRRRYNDLYRTEVLSLKEALFGEWKREILFFDDDNVTLTRKKGEVVMDGDVEVVKHKGMPILEGAEEFGDLIIEYKVIYPGGAKDQKIFRDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCP3</accession>
    <name>A0A1E3PCP3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycoside hydrolase family 132 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_27324</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SUN family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042181.1">
        <property type="nucleotide sequence ID" value="XM_019181357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCP3"/>
    <dbReference type="STRING" id="683960.A0A1E3PCP3"/>
    <dbReference type="GeneID" id="30198603"/>
    <dbReference type="OrthoDB" id="5339822at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051526">
        <property type="entry name" value="Beta-Glucosidase_SUN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005556">
        <property type="entry name" value="SUN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31316">
        <property type="entry name" value="BETA-GLUCOSIDASE-LIKE PROTEIN NCA3, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31316:SF2">
        <property type="entry name" value="BETA-GLUCOSIDASE-LIKE PROTEIN NCA3, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03856">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133820" description="Glycoside hydrolase family 132 protein" evidence="5">
        <location>
            <begin position="18"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="48"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="35954" checksum="464C50E53CFE1C5E" modified="2017-01-18" version="1">MKYSIIPLLALASSVLAAPHAHAHEHHLHRRDPAVVYVTQTQVAYVDGNGNPITPSTTATQAATSTSTSQDFEDPSEEFEDGKYKCSDFPSGQGVISLDWLDFGGWASVMNQNGDTSSTCKDGYYCSYACQAGMSKTQWPSQQPSNGISVGGLLCKNGYLYKSNSNKKSLCEWGQDSSYAVNNAKKSIALCRTDYPGSENMVVPTLVEAGSKKPMSVVKEDGYYNWRGGKTSAQYYVNDAGVSVEDGCVWGTEGSGIGNWAPVVLGSGYTDGTTYLSIIPNPNNKNSPNYSLKIVGQDGATAQGDCSYINGKYSGGPGSESDGCTVSVTSGTANFVFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCX3</accession>
    <name>A0A1E3PCX3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_76973</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042019.1">
        <property type="nucleotide sequence ID" value="XM_019184480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCX3"/>
    <dbReference type="STRING" id="683960.A0A1E3PCX3"/>
    <dbReference type="GeneID" id="30201726"/>
    <dbReference type="OrthoDB" id="428293at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015230">
        <property type="term" value="F:FAD transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044712">
        <property type="entry name" value="SLC25A32-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45683">
        <property type="entry name" value="MITOCHONDRIAL NICOTINAMIDE ADENINE DINUCLEOTIDE TRANSPORTER 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00784">
        <property type="entry name" value="MTUNCOUPLING"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="4"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="103"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="200"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ62812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="287" mass="32114" checksum="339AEE05E22B0D7D" modified="2017-01-18" version="1">MPITKTQREVLSGLTAGFITTTVTHPLDLLKIRLQLDISSKTHIEALSKIFKGFQNSASPTKEIYRGLPLNLIGGSSAWAIYFGSYRVFKDLLHDETLLDANLESWQYLISAFSAGSFTAVLTNPIWVLKTRILSTSKIAPGAYIGVIDGFKRVVKEEGILGFWKGLTPSLMGVSQGALQFTMYDTLKYRFRGDDGLGKLKVYEYIIMSCVSKITATLMVYPCQVLKSRLQDYESITKRKTVKQVVEGIWRLEGIGGFYKGVVPNIIRVLPATCITFGVYEQMRRIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PDK9</accession>
    <name>A0A1E3PDK9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_77106</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042154.1">
        <property type="nucleotide sequence ID" value="XM_019184502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PDK9"/>
    <dbReference type="STRING" id="683960.A0A1E3PDK9"/>
    <dbReference type="GeneID" id="30201748"/>
    <dbReference type="OrthoDB" id="5054775at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031930">
        <property type="term" value="P:mitochondria-nucleus signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013860">
        <property type="entry name" value="AreA_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021711">
        <property type="entry name" value="DUF3295"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053043">
        <property type="entry name" value="Ras-cAMP_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28014">
        <property type="entry name" value="NEGATIVE REGULATOR OF RAS-CAMP PATHWAY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28014:SF1">
        <property type="entry name" value="NEGATIVE REGULATOR OF RAS-CAMP PATHWAY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11702">
        <property type="entry name" value="DUF3295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08550">
        <property type="entry name" value="GATA_AreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitrogen regulatory protein areA GATA-like" evidence="2">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3295" evidence="3">
        <location>
            <begin position="809"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="593"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="665"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="718"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="813"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="936"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="271"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="472"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="498"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="605"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="675"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="723"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="738"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="942"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="968"/>
            <end position="978"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1010" mass="112918" checksum="A2A07BA2B80521FD" modified="2017-01-18" version="1">MSSRSNRINKAPPFLELSRDTIQNFDTSGLDLENVLSVWTGLTKCSGFIKSGKRLENISWRIVNRNLINQQNQQIPKGVDNNNNNTTQQRNKLNDGDFLSLLSIVTDQNLITQPRPQLIKKKSSNQKMARNASRNSNRSVESLDKLRPGMIPRKSSSSKSLKKLNQRTRRSNVDLPNLNRVYSNTSTTSDTNEQPVPIKVEHDSDLFEQPNPKYAFGVQAQRNDSNDDENNSSSSNFSNEKPQAPPLTRSDTSTSIVRGFSPSHISVAQLSTNSSPGKPSTSTKKAEKTSAPSLFDQSNLKNPQPRKAVPKSSLFNHPQPQQQQPHHNIQRIDTQTGVPLERVKSPLSQETTNEVLKPKEPNRSTSLFQQPQKSKPKTGTLFDHSALQENNNNVKSSLFEQVGISPEIKRQPTPQQHKKRTNVLNNLGMTSLAVKEHTPQRKDKNNMFFIESSPSPTDGPKYIDSQQQAQSHHNHHHHHHHHQQQRQRSSSSEKKRNNNNLSSSSVNDNNNNKSLFSGKSEPIPKEIIFSSDDSLSDESDWSSVSDSEGSDEFDENDLTKEWKQASFKRDEPLPKPQIKRSLLSGLFLNEMQHEHHHQHHHQEHSNSQIQSRSTSGVTTPQPWKSGYQHGDHSSAINLGANNNATGSQADLGTSISSSNIVVKHRHRPQSIHGGNNSNDAASNSTTEDNANNNNNDALELSSTTPHTVISTSNINVSGQVSEPQDHHDSQGKLKEKLTGTQEAQNNLQTSSSFTQMISRSAVNLTNYFAAHRKNSFSSIASDRTRTRFKHESNAPATASTLLPTALSTHMFLPNAHQRKSTRSKLASVLEAESGNTSSNEPSRKNSEAEESTVNTKIELPMKSPTVPITTKKPSHTVEIEGLTNGANGTNNHIEGAMETSANFNISRKLSPKTTRRQMLATELSDSLRKSILWDRKNGVPNNHHHHHHHHHHHQKQSNDNNEGEESKEEQSDVKETVDGSKQPKVIKSTIENFNNDEWDTAETDFYTRGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUB4</accession>
    <name>A0A1E3NUB4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="6">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_37109</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 10">
            <text>D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the class-I DAHP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="6"/>
    <dbReference type="EMBL" id="KV454220">
        <property type="protein sequence ID" value="ODQ56673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035880.1">
        <property type="nucleotide sequence ID" value="XM_019182518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUB4"/>
    <dbReference type="SMR" id="A0A1E3NUB4"/>
    <dbReference type="STRING" id="683960.A0A1E3NUB4"/>
    <dbReference type="GeneID" id="30199764"/>
    <dbReference type="OrthoDB" id="4699125at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000005">
        <property type="entry name" value="Phospho-2-dehydro-3-deoxyheptonate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006219">
        <property type="entry name" value="DAHP_synth_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00034">
        <property type="entry name" value="aroFGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009395">
        <property type="entry name" value="PRK12755.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE DAHP SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225:SF18">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE, PHENYLALANINE-INHIBITED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001361">
        <property type="entry name" value="DAHP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP synthetase I/KDSA" evidence="7">
        <location>
            <begin position="51"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ56673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="366" mass="40137" checksum="74BF89B060A49346" modified="2017-01-18" version="1">MFIQNQHIGDRTRLEDWRITGYDPLTPPDLLQHEFPLDEVNKKVILEGRDDAIKILNGQDDRLIIVVGPCSIHDPKAALEYQAKLKKIVDQVKGELLIIMRAYLEKPRTTVGWKGLINDPDIDNSFQINKGLRIARELFVNLTKTLPIAGEMLDTISPQFLSDLFSLGAIGARTTESQLHRELASGLSFPVGFKNGTDGSLTVAIDAMRSAAHSHHFLSVTKPGIVAIVGTEGNQDTFVILRGGKSGTNFDEESVKKAQSDLIAQGITSKPRIMVDCSHGNSNKDHRNQPKVAHEVAEQVKKGSESICGLMIESNLVEGRQDVPASGKQDLIYGCSITDACIDVDSTEAVLKELAEAVKARRALKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUG4</accession>
    <name>A0A1E3NUG4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosomal protein/NADH dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_107655</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035930.1">
        <property type="nucleotide sequence ID" value="XM_019180510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUG4"/>
    <dbReference type="STRING" id="683960.A0A1E3NUG4"/>
    <dbReference type="GeneID" id="30197756"/>
    <dbReference type="OrthoDB" id="1696305at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007741">
        <property type="entry name" value="Ribosomal_mL43/mS25/NADH_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040049">
        <property type="entry name" value="Ribosomal_mS25/mL61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13274">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13274:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05047">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00916">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein/NADH dehydrogenase" evidence="5">
        <location>
            <begin position="49"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ56723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="144" mass="15914" checksum="0E0798A479F812E1" modified="2017-01-18" version="1">MSAQSMFPGLTASRFTRQITRLNLISSGPGSVTVGQNVKNVELLFKRLNTDGHMGARKFWRENLPRVQFHNPALPIQVTRVEVANASEHAKVPAILKVTYADGKTAQVDVKNKHSSDILNDFINLTGAKPAKEVIIDRDPRGIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUM2</accession>
    <name>A0A1E3NUM2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_65761</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036086.1">
        <property type="nucleotide sequence ID" value="XM_019183983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUM2"/>
    <dbReference type="GeneID" id="30201229"/>
    <dbReference type="OrthoDB" id="10664957at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="269"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ56879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="431" mass="50880" checksum="A922B090BEF54DEA" modified="2017-01-18" version="1">MTSISPDRNTVYDYDLSTLSNIRLDNRRAKQKLSNNVLHQANPSATNRYNYNPTRSRKSKHDYQLDETADPIRLFKDSPSPNKYDYKYNNISPFKYNSSISRKYDIDSSLLEKDNLKYLDRDYYSNGSKLNEQKRERFLDSINSANNTKFSNRQSSHYDVSPVRDTITRKPYEDLGFRPRNKDIGIPNLKPDNYYSNPFSKSPIKAGTSGFSKSDIYNDNIDPDRLDRESEEQLRNYDKRFQHLVDKYQLDELSAHEDKIASSEPESKLDRYRSMIRKLQAQNQQEANNNQQLKEKITELEKFIEFLESNFDQLYKRSGILKLEKHDIHRKNQELIFINNYYKSLLRKQKIYKQSEDTTISLLDHKFEDDDSTTQLLINFNTTKPSATFNKLSSIKKFRALGFAVLASVKLQHRLQHRQAYIKKVHDLMGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUN8</accession>
    <name>A0A1E3NUN8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein acetyllysine N-acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.286</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_36884</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.286" evidence="2"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036055.1">
        <property type="nucleotide sequence ID" value="XM_019182483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUN8"/>
    <dbReference type="STRING" id="683960.A0A1E3NUN8"/>
    <dbReference type="GeneID" id="30199729"/>
    <dbReference type="OrthoDB" id="420264at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099115">
        <property type="term" value="C:chromosome, subtelomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031934">
        <property type="term" value="C:mating-type region heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005721">
        <property type="term" value="C:pericentric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046970">
        <property type="term" value="F:histone H4K16 deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045950">
        <property type="term" value="P:negative regulation of mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031508">
        <property type="term" value="P:pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01408">
        <property type="entry name" value="SIRT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017328">
        <property type="entry name" value="Sirtuin_class_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF6">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACETYLASE SIRTUIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037938">
        <property type="entry name" value="SIR2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0520">NAD</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="13">
        <location>
            <begin position="4"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="289"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="289"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="304"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="314"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="332"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8 11">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="30"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="40"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="112"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 11">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 11">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 11">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 11">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="212"/>
            <end position="213"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="236"/>
            <end position="238"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037938-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037938-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR037938-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ56848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="342" mass="38778" checksum="D242C9E49242D9CF" modified="2017-01-18" version="1" fragment="single">SIQMSISQKDLQKVAAILKSSPKVIFMVGAGISTGAGIPDFRSPKTGLYHNLQSLDLPYAEAVFDISFFKSNPKPFYTLAKELYPGQYVPTKFHFFMKLLQDKKLLHRVYTQNIDTLERVAGVEADYIVEAHGSFAENHCIKCHKEFSGDDFKKDVFDKKIPRCKSCKGLVKPDIVFFGEGLPERFFNQWEEDLEDLESAKDGEFLTITAGTSLTVYPFASLPSEVPKNQKRVLVNKERVGDFKSKRENDVVFLDDSDEFVESLCDLLGWRKELDSLIAKGTKDIKGIKHETAEEKAKEITEDVKEELEEEAKEDEKNLQDLTKDLGTLNLEKSRDPTSKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUP4</accession>
    <name>A0A1E3NUP4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Probable metalloprotease ARX1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Associated with ribosomal export complex protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_86210</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Probable metalloprotease involved in proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Accompanies the pre-60S particles to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M24 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035957.1">
        <property type="nucleotide sequence ID" value="XM_019186014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUP4"/>
    <dbReference type="STRING" id="683960.A0A1E3NUP4"/>
    <dbReference type="GeneID" id="30203260"/>
    <dbReference type="OrthoDB" id="5876363at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047113">
        <property type="entry name" value="PA2G4/ARX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804:SF102">
        <property type="entry name" value="METALLOPROTEASE ARX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804">
        <property type="entry name" value="PROTEASE FAMILY M24 METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ56750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="599" mass="65428" checksum="DD9EA6D53C0F2083" modified="2017-01-18" version="1">MELAIAPQDQDILLRDKNVINESVLNKYRLAGQVSQTTLTYLTKLINDSYHLGKGPKISTDELCILGDSFLASSLESVYSKGSNSVNEKGIAQPVTIDVNNVSNGFGPELDDDHKNFKHFEAGDIVTITLGCHIDGYTSNLSHTLVIYPPGEKTQENDEELRPTGPLLGPKADAVVAAHIASETVIALLGVSLTPEKFPTFLKTLNNGSNSITGSLIRSAVDEIAESFKCIVVPGSRVRRIRRFLAGQAEGVVAEKEFKGVVWSEGDQEATLLQQAADNASKDLVKLDKDVKGTGVNQISAIPTDDFVVHPGETYVVDIKMAPISEFNEVGLVTLQTVDGYTGKNHDESSLNIKPSIFVRDFAMQHTLKLRTSRQTLTKIDKTRSVYPFKLSHLSDKFPINIKAGSGYGSEALNQDIESIKKDIKSSRLGLTEILNNHLAVAKPIQTVRFLPLHVILNATNKTGSHGFDAETPTLPGLELPLPRLGISSLKLKSLLKKNSLPIPVAREQSTVVLIQSGISSGGRPEVLRLTGGSKTAKPSWVHSNYQLNETRILIQGAIELIKLSQDKRFGINIRECQPMKLTNVLSFDNKPEEQMNID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUR4</accession>
    <name>A0A1E3NUR4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dynamin GTPase</fullName>
            <ecNumber evidence="3">3.6.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">WICANDRAFT_86303</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.5" evidence="3"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036092.1">
        <property type="nucleotide sequence ID" value="XM_019186034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUR4"/>
    <dbReference type="STRING" id="683960.A0A1E3NUR4"/>
    <dbReference type="GeneID" id="30203280"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030061">
        <property type="term" value="C:mitochondrial crista"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097002">
        <property type="term" value="C:mitochondrial inner boundary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901612">
        <property type="term" value="F:cardiolipin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140523">
        <property type="term" value="F:GTPase-dependent fusogenic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180020">
        <property type="term" value="F:membrane bending activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070300">
        <property type="term" value="F:phosphatidic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015886">
        <property type="term" value="P:heme transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097753">
        <property type="term" value="P:membrane bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097749">
        <property type="term" value="P:membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990627">
        <property type="term" value="P:mitochondrial inner membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990626">
        <property type="term" value="P:mitochondrial outer membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000741">
        <property type="entry name" value="Putative mitochondrial dynamin GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056495">
        <property type="entry name" value="LIS_MGM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF212">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24550">
        <property type="entry name" value="LIS_MGM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13 17" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="20">
        <location>
            <begin position="226"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="19">
        <location>
            <begin position="780"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="166"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="170"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ56885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="894" mass="99725" checksum="FD2FA8C28AEBDF19" modified="2017-01-18" version="1">MRPLWGGSNASRALHRGIFKISPLFVLKQNYSVLNRRFLSHSPLLLSSAASRILQRGNTTHLLGHRPFSFTSIPKILGKAVKAPAYLGGSLAAAGSYVAYKVEEASNYTQDRLNQMKEFGGNAYDKASDFFNGLNFGSGAGGEGGGSGGNGGDSAAAVGTAAAIAGVNSQEEEEEATDEEEKEELIDQDEDDEPEDDSTDDQIMSLTRQMIEIRTLLQEIDRSSDTLKLPSIVVIGSQSSGKSSVLESIVGQEFLPKGSNMVTRRPIELTLVNTPNTAAETADFPALKMFNLTDFSEVQKILFDLNMAVPATEAISSDPIQLTIRSPTIPDLSLVDLPGYIQVEAADQPTVLKQRIRELCDKYLEAPNVILAISSADVDLANSSALRASKIADPKGERTIGVITKLDLVEPQVARNILLNKKYPLKMGYVGVITKAPTKSLFRKSVGHDAFVAQQNFEYQYLKTNKEEFGQCNVSTRILKKKLIKVLERTMSMSLAPTASLVQQELDETAYKFKVEFNDRQLTPKTYLAENVDTLKLAIKEFNEHFGRSELRSILRNELDQKVLDLLAARYWNKPSEDEVGTKVLIENLTALNEMKKDDSYWLKKLDLTTSSLTKLGVGRLSTTLITNAIVSEVENIVDGTQLKNHPLAKEAVKEAAQSVLNSRYFSTADQVENCIKPYKYEVDVEDREWTISREHSIQLLKEELTQVNDTLQVLKTSIGGRKLSQVIKYLDEGGKNHEETLGFSKVLLDRGREAQFLRDRSIILGLRLKALKSNSCKTKDNKFKCPEVFLNVVSEKLTTTAVLFLNVELLTDFFYKFPRELDSRLNINLTEDQIEEFAKEDPKIKRHIELQQRKELLELALQKIDGILAFQRNKSGLSTNYRNNNNDGNGKWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUR9</accession>
    <name>A0A1E3NUR9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ribonuclease T2-like</fullName>
            <ecNumber evidence="4">4.6.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">WICANDRAFT_37033</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Rnase which modulates cell survival under stress conditions. Released from the vacuole to the cytoplasm during stress to promote tRNA and rRNA cleavage and to activate separately a downstream pathway that promotes cell death. Involved in cell size, vacuolar morphology and growth at high temperatures and high salt concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the RNase T2 family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.19" evidence="4"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036085.1">
        <property type="nucleotide sequence ID" value="XM_019182505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUR9"/>
    <dbReference type="STRING" id="683960.A0A1E3NUR9"/>
    <dbReference type="GeneID" id="30199751"/>
    <dbReference type="OrthoDB" id="435754at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005775">
        <property type="term" value="C:vacuolar lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033897">
        <property type="term" value="F:ribonuclease T2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01061">
        <property type="entry name" value="RNase_T2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.730.10:FF:000004">
        <property type="entry name" value="Ribonuclease T2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.730.10">
        <property type="entry name" value="Ribonuclease T2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033697">
        <property type="entry name" value="Ribonuclease_T2_eukaryotic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001568">
        <property type="entry name" value="RNase_T2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036430">
        <property type="entry name" value="RNase_T2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033130">
        <property type="entry name" value="RNase_T2_His_AS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057328">
        <property type="entry name" value="RNaseT2L_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240:SF22">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00445">
        <property type="entry name" value="Ribonuclease_T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25488">
        <property type="entry name" value="RNaseT2L_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55895">
        <property type="entry name" value="Ribonuclease Rh-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00531">
        <property type="entry name" value="RNASE_T2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="RNase T2-like C-terminal" evidence="19">
        <location>
            <begin position="286"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="253"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00071169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR633697-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF25488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ56878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="391" mass="43766" checksum="AC9C309FBE2A1274" modified="2017-01-18" version="1">MAQLEYIRDLSSETTFDNSKCPSSIPLSCSNDTVVKDSCCFEYPGGVLLQTQFWDYSPSIGPNDSFTAHGLWPDNCSKDGSYQQFCNSKLNVGNVEQVLQSFGEDELIANMKKYWKNMNGDDASLWKHEFNKHGTCVNTINPSCYGDDFKTNQNVVDYFKIAMNLYEKLPTFEWLSEAGIVPSHDKTYTKDEISKALADKFGSEVYIACDRNNGLQEVWYFHQLKGSLLGEQFHHIDSFSNSKCPNQGIKFVPKDNYKNPGTNPSQTKTSSPPKPTGDYQTGFVKLKNQQGCLIKNGQWYVSGTCATYRLQAAEFGGYNLRSNGGYCALDNNGDLSCSQSNEPFQFGYDESSKLLSVNGQETWHADKTPSGFQKVPVAPGDGEVQFNLKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUW2</accession>
    <name>A0A1E3NUW2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I, II, and III subunit RPABC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_36801</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the archaeal Rpo10/eukaryotic RPB10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036091.1">
        <property type="nucleotide sequence ID" value="XM_019182475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUW2"/>
    <dbReference type="STRING" id="683960.A0A1E3NUW2"/>
    <dbReference type="GeneID" id="30199721"/>
    <dbReference type="OrthoDB" id="10258858at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000024">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00250">
        <property type="entry name" value="RNApol_arch_Rpo10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023580">
        <property type="entry name" value="RNA_pol_su_RPB10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020789">
        <property type="entry name" value="RNA_pol_suN_Zn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000268">
        <property type="entry name" value="RPABC5/Rpb10"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003089">
        <property type="entry name" value="PRK04016.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431:SF3">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01194">
        <property type="entry name" value="RNA_pol_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005653">
        <property type="entry name" value="RNA_pol_N/8_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46924">
        <property type="entry name" value="RNA polymerase subunit RPB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01112">
        <property type="entry name" value="RNA_POL_N_8KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ56884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="75" mass="8830" checksum="C16704677D894209" modified="2017-01-18" version="1">MIVPVRCFSCGKVVGDKWESYLKLLEDDTTANEGQALDKLKLKRYCCRRMILTHVDLIEKFLRYNPLEKREVVPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV21</accession>
    <name>A0A1E3NV21_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_22060</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036195.1">
        <property type="nucleotide sequence ID" value="XM_019180928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV21"/>
    <dbReference type="STRING" id="683960.A0A1E3NV21"/>
    <dbReference type="GeneID" id="30198174"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005619">
        <property type="term" value="C:ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072687">
        <property type="term" value="C:meiotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030437">
        <property type="term" value="P:ascospore formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="4">
        <location>
            <begin position="13"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ56988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="295" mass="33767" checksum="1EF6775694FC27E8" modified="2017-01-18" version="1" fragment="single">SPEEIRRRKILKKGIQFTLLVCGEEGTGKASFINTLCNQEVISKTESKNYYPPNAHLNPGLNILKKHVDIIEENATPISLEIILTPGFGDNINNNDCTGKVIDFLEQQFDSVLREECRIRRNTKFEDGRPHACLYFIRATSKGLRELDVEAMKNLSTRVNVIPVISKSDTLTEDELSLNKALILQDIRANGISVYDFSLDIDDDSESVDEQMFLKNGLPFAVSGSYETIVDDNDDISHIREYPWGTFRVEDLNHSDFNYLKNVLFGSHLQELKESTHSVLYENFRRIRLLENTKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV25</accession>
    <name>A0A1E3NV25_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Shugoshin N-terminal coiled-coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_97560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036258.1">
        <property type="nucleotide sequence ID" value="XM_019186592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV25"/>
    <dbReference type="STRING" id="683960.A0A1E3NV25"/>
    <dbReference type="GeneID" id="30203838"/>
    <dbReference type="OrthoDB" id="3981157at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="330" mass="37980" checksum="435A1283319E8A95" modified="2017-01-18" version="1" fragment="single">MTKVADLEIKISDLIQQNIELRAINAKSEDQKRKWLEDKLNIIENGVSQRFEEMFQMFATIRSNEGLASSSVNVNNVIQNVALENSSDKTVSFHDVKSPVKKNISERRRKSARRQSMYIAPPPSPPQDHFNDEANEEDFERVQIPDDFNTPSVFQDEQNYEEVQESNEPQINDAFIAEEDAETEEPELKEHDEAEHAATLSPIKFSSPKMPEYHYPKFSVYTETEDDNEAPNKEEIDLKIQKLAAGSVKSGQTSEKEKQENEEAKDELEMIDFTDFNEKTKNLLFQDNDKIKHTKSKKQKQKSSKDETMPEPISSDMLSTRKSRTRGKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVA9</accession>
    <name>A0A1E3NVA9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_65289</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_019183888.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3NVA9"/>
    <dbReference type="GeneID" id="30201134"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF37">
        <property type="entry name" value="AMINO ACID TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="366"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="395"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="439"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="474"/>
            <end position="495"/>
        </location>
    </feature>
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        <location>
            <begin position="45"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="ODQ57024.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="546" mass="59917" checksum="5E171613B0CF3E77" modified="2017-01-18" version="1">MSSSIEDHEKSAGTFKVQSDHDDIDVQSFNEVEEAKLKKIMSSRHLFMISIGSAVGMGLWLGVGGSLHKGGPVGVFIGYLISGSIAWSLNMSIGEIATLYPVSSAFPRWSAQFIDPSIAVTVGWMHWWGNMIGASNELVGIATVMKFWVPDVNPGVWITVAFVCLVLIHAFAIRLFAEVESLMSGVKFLWVIVAIVTMIVISAGGAPQGQAIGFQYWKSVPFNGHGFKGFLTVLPSCVFAMAGIEIIGLVAAECKSPRKSVPKAVKSIWIRIGLFYILGSFVVTIVLDPNNSQLLGGSGVNASPFVIAAQNSGLPRFADAMNAIILISVFSSGSATFYGCPRILLGLVHLKMAPKILGKVDKSGRPWVGYIFTGILSGGLAYINLNHSGSTVFGWFSNLCSLVALLLWSIIFIVNIRLRKAWVLQGRSLNDLPWKCKSVPYTAWYGLFWSILIVIIEFYLAVWPWKDKSSAKNFFANYVSIVLMVFIYSIAKIFFFKGPLLIPLEQIDLDKGRAIYDDDYMANEGEDLGVFRGAIYDLRHAFDKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVR0</accession>
    <name>A0A1E3NVR0_WICAA</name>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
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                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
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                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57299.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="104" mass="11300" checksum="B8BB68F14181C70C" modified="2017-01-18" version="1">MSHQMPGAFPSTPRSNARAFDQKTPTAADFKFTPISSMSGVKKPLYPNISHLQQGQGNELEKEFQDLGYSNTLSTKVLSELNERAASKAPTSNTGNNLLSPRST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVV2</accession>
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    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
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                <person name="Stielow J.B."/>
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                <person name="Kurtzman C.P."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="338" mass="39900" checksum="B25EECB0495818F1" modified="2017-01-18" version="1">MISKLPVELKLAVLELSPNLRYANTEFYILYNELFKMKTLHLVGDEPLIKISKLLQFFSSTFEFWNGALTKLYRPDLNWVLIYSLLKNRKIFFQKEDFVIQADQNNIEQELDTEYVHLQDVHAFHYHKIVLLPNGNYNFQVALRNLHSSRGLGTTRFQLNYLDESFTAYPPSVINEILPKSQLSVMNLGEFTINNPEDALTEVEIIIEEIGMYPKCDLIFDYLDFKPINCDYLYYTIPGDPFKEYNKFVNKVEKACHLAIDEIWNHPESPDVSPVDSLESFNEIVDQDDDELHSLKKYSKEFYTKCSRSVKMYYPSDQRHFREKKTGKLLDWRVPLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWD4</accession>
    <name>A0A1E3NWD4_WICAA</name>
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            <fullName evidence="7">ABC-2 type transporter transmembrane domain-containing protein</fullName>
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    </protein>
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        <name evidence="8" type="ORF">WICANDRAFT_80784</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
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            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036655.1">
        <property type="nucleotide sequence ID" value="XM_019185122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWD4"/>
    <dbReference type="STRING" id="683960.A0A1E3NWD4"/>
    <dbReference type="GeneID" id="30202368"/>
    <dbReference type="OrthoDB" id="4095361at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="ABC-2 type transporter transmembrane" evidence="7">
        <location>
            <begin position="3"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="294" mass="33052" checksum="C3154116CC55A4A1" modified="2017-01-18" version="1">MIFNPLLEQGLPTFVEQRGLYETRERPSKTFSWKAFIAAQITAEIPWNALVGTAAFFCFYYPVGFYNNASPSGTAAVNQRGAYAWFFNVLFYIYIGTTSHLVMAPLELTESAGNVGYLIFTLGLTFCGVLVSLDKLPGFWKFMYQVSPFTYIVDGFLSNGLANNRVQCSSTELVRFSPSTGYNCAQYMESYITKAGAGYLADPFATENCEFCAISSTNDFLRVVGVSYDRRWRNVGIFIAFIFFNIIGATFLYWLARVPKKADRVKCVKKEGEVNVPQSEYSKSMIDKEDSTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWI6</accession>
    <name>A0A1E3NWI6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 22">Folylpolyglutamate synthase</fullName>
            <ecNumber evidence="6 22">6.3.2.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20 22">Folylpoly-gamma-glutamate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19 22">Tetrahydrofolylpolyglutamate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">WICANDRAFT_85646</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Required for methionine synthesis and maintenance of intact mitochondrial DNA. Involved in telomere maintenance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + L-glutamate + ATP = (6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10580"/>
            <dbReference type="Rhea" id="RHEA-COMP:14738"/>
            <dbReference type="Rhea" id="RHEA-COMP:14740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:141005"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="22">
            <name>a monovalent cation</name>
            <dbReference type="ChEBI" id="CHEBI:60242"/>
        </cofactor>
        <text evidence="22">A monovalent cation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 22">Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 22">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.17" evidence="6 22"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036701.1">
        <property type="nucleotide sequence ID" value="XM_019185919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWI6"/>
    <dbReference type="STRING" id="683960.A0A1E3NWI6"/>
    <dbReference type="GeneID" id="30203165"/>
    <dbReference type="OrthoDB" id="5212574at2759"/>
    <dbReference type="UniPathway" id="UPA00850"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.10:FF:000009">
        <property type="entry name" value="Folylpolyglutamate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023600">
        <property type="entry name" value="Folylpolyglutamate_synth_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF5">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038895">
        <property type="entry name" value="FPGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01012">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 22" id="KW-0436">Ligase</keyword>
    <keyword evidence="15 24" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 22" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="24">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00025142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00030592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00030876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRNR" id="PIRNR038895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR038895-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR038895-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="ODQ57494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="467" mass="52134" checksum="F61945C0266A955D" modified="2017-01-18" version="1">MRRSYTDAINALNSLQSNFATIEAIRKSGINKNAMAMPEMLEWTRRIGYKSSDFNKLNIIHVTGTKGKGSTCAFTQSILGQYQPKIQKIGLYTSPHLKSVRERIRINGQPIAEDLFTKYFFEVWDRLSSTNSNETEFPHMGSDIKPMYFKYLTLLSFHVFLNEGVDTAIYEVGVGGEYDSTNLIEKPTATGVSALGIDHTFMLGNTLGEIAWNKGGIFKTGAKAFTVKQEPEGMKVLEERAQEKGVELKVIDESDVKGLELGIPGSFQAQNASLAVSLANEHLTKLGIQIDSEKTREGLKKTFWPGRCQIIKEDKITWFIDGAHTKESIDASSGWFKTATDPTKKRVLLFNQQTRDANALVTRLFDGVSPLKFDEVIFTTNKTWSTGYSKDLVSMNTSKEAVDSLEVQRLLADEWVKLDSDSVINIFHDIEESVEYVRSLDGDVDVFVCGSLHLVGGFLVVLDGDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWJ2</accession>
    <name>A0A1E3NWJ2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_85659</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036715.1">
        <property type="nucleotide sequence ID" value="XM_019185923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWJ2"/>
    <dbReference type="GeneID" id="30203169"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="463" mass="54668" checksum="392F41AB1590E07D" modified="2017-01-18" version="1">MSHFPVDNYTVFPLEVLTKIFRIYIDSDDQSKLMSKNYTNVLSLNRTFLEIFGPERIMKADFYALIPLRSTNYVKSFANDEEYKKHMFREYRFREFIFNLRIPRRNVPPTAPVHIIRREWENLQWITAPRQLELYNRAFSSAYEVLDLEFHPDYIEKHFPEMQSRKEQRDTQVFKSVKSLLLFCTRVMNNENSIMKKYVKSFNIDLLFLDEFQTARTSKHTLFGKILEKYSVGPDNNYLSIKPKPFKVFSNFFGTSLEHFHKSTSSTALDMYRGSLEHRRASSRAEYISASFQLSADFLFHRQYLDTSGSNTSNHLEIQNRWPIFKQILLTSGFLQLFCENKRMNLANCVSKKEAQSVLNDVFETFDRFEGIRIKRRINLAVMLRHRDMEHNQAFCFAHCFFKAFNNLVEELVKACTINSSDHISTSLVTLGLKTNANDWENKEAFELIKPKMIVINKPARSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWL8</accession>
    <name>A0A1E3NWL8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="8">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_65344</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="8"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036289.1">
        <property type="nucleotide sequence ID" value="XM_019183893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWL8"/>
    <dbReference type="STRING" id="683960.A0A1E3NWL8"/>
    <dbReference type="GeneID" id="30201139"/>
    <dbReference type="OrthoDB" id="1470711at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17932">
        <property type="entry name" value="DEXQc_UvrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.486.10">
        <property type="entry name" value="PCRA, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070:SF46">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE HMI1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="12">
        <location>
            <begin position="36"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="13">
        <location>
            <begin position="297"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="57"/>
            <end position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ57082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="678" mass="76619" checksum="CA9903AEB6922159" modified="2017-01-18" version="1">MSFSQIEPTDSQAGQAEQQAEQDSMVEFHDYEVQVTPSQSKVITHPVDTATTLQVVAGPGSGKTTTLVYKIAYMIGNLGINPSEILVLSMTNKAVEALQTQLSNILGENNSVDVTTFHGYAHRCVINNDQSQENGIQIIEDAGWRTLVRLLGNKVNKYQLIEIVDQIRKDGWNEKYLQNVCRKFKLEMTELKDVMQTLDTSNVLIHSDVMRGAKAYIRDGRVSLPYKVVIVDEFQDVYPGIYELVELIARNAHLITAGDPYQSIYGFLGSNSSVHKTIKNFKPIDKLYLSESFRSTPEIIEASDNLMKREAPSLALKGISGFKPVAKLFTDVINQHEWVATEILRILEDSEGVVEPNDITILAKTNGELSRFKTCLNFYGLLDYKLSSNPSWLSDGLFHLIDYLRILFAPESSNFPVLCTIALIPGVGPVTIKKFHKGALTSELSMWEYLRNLREKNSLPPKLIPYFDLIEETRQKINFDDADSIFNHLLHLGNELGLRKEVTKTIKTIADKDNINNQLLDFFENLKLSNNFKAADTFLTEHFLRNYMNSLPISKTKNSVKLSTIHSAKGLEFPIVFVLGSTTGDDYKPESKNLVYVAMTRAKTLLYMNMMKTSDPFSATNFKKRFSEKYFTHDKPQIRGAFTAGFAKEFNRTFNGGAISKQNVVRSFHTLSKFIKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWQ6</accession>
    <name>A0A1E3NWQ6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">tRNA (uracil(54)-C(5))-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_35163</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in all tRNA. May also have a role in tRNA stabilization or maturation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>uridine(54) in tRNA + S-adenosyl-L-methionine = 5-methyluridine(54) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42712"/>
            <dbReference type="Rhea" id="RHEA-COMP:10167"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74447"/>
            <dbReference type="EC" id="2.1.1.35"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.35" evidence="5"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036781.1">
        <property type="nucleotide sequence ID" value="XM_019182276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWQ6"/>
    <dbReference type="STRING" id="683960.A0A1E3NWQ6"/>
    <dbReference type="GeneID" id="30199522"/>
    <dbReference type="OrthoDB" id="10250660at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051908">
        <property type="term" value="F:double-stranded DNA 5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030697">
        <property type="term" value="F:tRNA (uracil(54)-C5)-methyltransferase activity, S-adenosyl methionine-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000201">
        <property type="entry name" value="TRM2p tRNA methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000174">
        <property type="entry name" value="TRM2p tRNA methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030390">
        <property type="entry name" value="MeTrfase_TrmA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030391">
        <property type="entry name" value="MeTrfase_TrmA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025795">
        <property type="entry name" value="tRNA_(uracil-5-)_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061">
        <property type="entry name" value="RNA M5U METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061:SF30">
        <property type="entry name" value="TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05958">
        <property type="entry name" value="tRNA_U5-meth_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51622">
        <property type="entry name" value="SAM_MT_RNA_M5U_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01230">
        <property type="entry name" value="TRMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01231">
        <property type="entry name" value="TRMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TRAM" evidence="12">
        <location>
            <begin position="89"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="517"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="517"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="490"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ57574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="565" mass="62972" checksum="B2B4B4809D07A4A7" modified="2017-01-18" version="1">MEPVTSNPKRELSPVGSPPSTIKKPTPNPIQQKKKKEKKRKYKTKPIDKTSPLGILQFEIQEILKTHNLSPEQIINDINTLLNTPSLTSTYHRDVSNVHVLQTSSNGDGLALVPHPIVPEHRQVVIIPFAIQGDVVSIRIFKTHPNYVEADLLSITTASPLRDDSLIRCKYFGKCSGCQYQNLSYPQQLEFKRNTVHNAYKYLAPNLIAQGKVPQIGETVGSPLQYGYRTKLTPHFDAPRKGCLSYRPSLGFGRKGRPEWRDGDAGQSGDQSVLDIEECVIGTKIINLGMGNERAKFEEGWKSYKKGATVLLREHTRVGTREQSQALEYCNEQDGSKDSNGNVSVDLVEGDDEGDKVLVKSCVTQSRQIVTEYINGFTFEFSAGEFFQNNNSILPKVTEYVCSNLQIPGKSKESPLYLVDAYCGSGLFSITASKGVDKVVGVDVSADSIKFATRNAERNGVLNTEFIDGKAEKIFEKIDTPSDLTSVIIDPSRKGCDEVFLNQLSEYKPARVVYVSCNVHSQARDIEWFLNNTTNGPEYKIESIRGFDFFPQTHHVESVAVLSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWW9</accession>
    <name>A0A1E3NWW9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="8">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_81413</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Lipid-anchor</topology>
            <topology evidence="8">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="8"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036399.1">
        <property type="nucleotide sequence ID" value="XM_019185231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWW9"/>
    <dbReference type="STRING" id="683960.A0A1E3NWW9"/>
    <dbReference type="GeneID" id="30202477"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000038">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF10">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 8" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="568"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="11">
        <location>
            <begin position="421"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="538"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ57192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="591" mass="65439" checksum="3C95C7249E98719A" modified="2017-01-18" version="1">MMMMISCANAFNLCLQFAAIYTCSLFANPIDQLTGNYSASTSSIAQISSSNSSSDSKSYSNLAPIEAYGNKFFNSEDGKQFFIKGIAYQPSRAAGDVIALNADNVKFIDPLAEPEICLRDLPYLQKLGVNTVRVYSIDTDRDHDVCFEAFAKAGIYVIADLSEPDVSVVRDSPTWDTTIYNRYTKVVDSLHKYPNVLGFFAGNEVTNDKSNTFASPFVKSSIRDIKSYIESKGYRKIPVGYSTNDDAETRANVADFFTCGDVAADFYGINMYEWCGYSTYWASGYKERTEEFRNYPVPIFFSEFGCNVKRPRPFTEVEALYSRLMTDVWSGGIAYMYFEEPNQYGVVQVNGGDVEELEDFKYLQKQFADVDPKGATLDEYKVTFKAAKAPSCPSSSKFWQASADLPHTPDSTKCECMENSLQCGLSSYVKSNNLTHIFDYACNIVDCEAIKGDGSTGSYGFFSDCNARQKASYVINEVFLNLGGKKDKCDFDGLASLNSKVSTEQELSDIVTLTGDSCLSIIKANEAKKLELKSFSKKNSTASGNSTNSRNGHGNGTGHENSGSQIKFYQNLSSIVLLSFVFVIILPFLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWY5</accession>
    <name>A0A1E3NWY5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribosome biogenesis protein ALB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_35143</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036865.1">
        <property type="nucleotide sequence ID" value="XM_019182273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWY5"/>
    <dbReference type="STRING" id="683960.A0A1E3NWY5"/>
    <dbReference type="GeneID" id="30199519"/>
    <dbReference type="OrthoDB" id="4086742at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022784">
        <property type="entry name" value="Ribosome_bgen_Alb1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09135">
        <property type="entry name" value="Alb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="17724" checksum="6AC777485993B210" modified="2017-01-18" version="1">MPSRNSINKPKDTMNQARKRAQLAKKRQARARNGLTAPARSSESSTSGLPKSTAVALYTGSKPTGSITTSTLSKKRLHKIERNKKYAAKRKEIENKLLIDATAQAEEQMDVDDDNESVASSKKPSSKIDSVKNALWSVIEDRASESLTVDSTGEGTTLGGPSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXP2</accession>
    <name>A0A1E3NXP2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_101991</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036967.1">
        <property type="nucleotide sequence ID" value="XM_019180339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXP2"/>
    <dbReference type="STRING" id="683960.A0A1E3NXP2"/>
    <dbReference type="GeneID" id="30197585"/>
    <dbReference type="OrthoDB" id="282973at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00842">
        <property type="entry name" value="MPP_ASMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041805">
        <property type="entry name" value="ASMase/PPN1_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340:SF27">
        <property type="entry name" value="ACL091CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133546" description="Calcineurin-like phosphoesterase domain-containing protein" evidence="3">
        <location>
            <begin position="19"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="4">
        <location>
            <begin position="266"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="631" mass="72252" checksum="7837B9ADDCE9D9FC" modified="2017-01-18" version="1">MKFSSLFIGAIASSIASAVPFFNGTNTTKCDKCIKRLQLGKSIVSTKPNLIYPIFTKWCTDNKIHDPTTCKLDYGRNTVDNATFGTNFANMLYLMEPSSYDGQLYCHYQDSKSCPLPKTPKIDMSSYWPAKKPEHEVAPEVGNETYNVLHVSDFHIQLDYQIGSEANCSQYMCCTGHNFNKHSPPQGYNYTANLTHDQIANLSFTPAWYDADYELQHGDPIEHVLSNGSVWVPANTFGHYACDAPEVLINSSLKSVAQYQEELGLDFQFSIFTGDLVDHDEDQFLDYEKNIKSEEYIFRDIKATLKDIPLYAVLGNHDTYPYAQLAQQKSGFANLFDWNAELMADMWEDYGWLNSTTAKYAREHYTGFAVTTKTDLKVIALNSNAYYASNLYNYWNATDVDSFGQFQFLIDELIDSESKGQRVWIIAHIPFVEEALPVPAEVFTQIVKRFSPYTIAGIFFGHTHLDQFNILYDGPVTNKSEESVSNFAWIAQAVTPLTDNNPSWRYYTVDKKTHSIMNSYNYYTKLNDTFYNESDEPVWEFEYSARESYANVTEWPETAPLNATFWHRVANGVNASDEVSQTYNNYKRRFSPYVPECIGTGNCDGSYCFLASFTLKDYEECMETFHEPEFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXQ0</accession>
    <name>A0A1E3NXQ0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_106384</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037176.1">
        <property type="nucleotide sequence ID" value="XM_019180474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXQ0"/>
    <dbReference type="STRING" id="683960.A0A1E3NXQ0"/>
    <dbReference type="GeneID" id="30197720"/>
    <dbReference type="OrthoDB" id="2943660at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF12">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="14"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="117"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="859" mass="99009" checksum="7629886BE15486A6" modified="2017-01-18" version="1">MNGAPVKRQRPNKSCTICREKKTKCDREKPICGTCKKRNQSGLCRYEGPEWTIKVGDQNFVTPVQSSVKKPRVKKHTQEQLPYQRQFQVNNVRIPQTRTESVKSDDAKYTNRLRVESLLSPSAPPSTYSASGNSDRAAHSMDGDDADGDDILDFYKDNYVDPGVFADGTLTWVTLLKKDIYSRALVICFNKNAILKKIKNSQMDDHYFTNKFMAEDDTDSAVPECPAKTSEIAGKNFIESLRDELKDNKLTWLLIEKFFESELYCNLPILDKEDFTEQISRIIPPRDSKSGDNKLNITGKFQLAALGILLMVMRFSSLSVYNAGKPLPQPISPNDQYIVDHPVTKDVVTLAKACLEELSVLKQSHLVIFQLSLLLQHYNFFAPEDCDCITASTITNMGPLLHHAISSGLNRDPTVSRPKYPKPNLLRRLWYLLEYMDYYQLMLVGYAPIINHSFHDTQIPILDHNDSALQYVINEAFHDRRKIHEVCRPLLMLILNVREPPKIRDVLNQMKGLEEHVEKIPKYDELLRMPSDTVIHRYNKLRIHNGTADSCALLFMIYYNFFLHYNDKRNSEKSLYYMLQLLRISKYLYTSVHFLRKPDEQNEKFNLNKHFGIGILALPKIELCLHKISEFMIALLGRIKVHKALLGLNVDQQRLELLDKIGRLTFKICYFIVDACANISDTYYHAWSISKIQSFIIEKVFINREGRLGPHSLTQIEAHYVEVVEELRSQDDGFFLYSTNDLKQILNALELMDTESLGQTPSPERLLTDLNSYKSKNDQLWFDQMMFENSFNVNEGGNNSTNFNGFSNNNGIGNNTPVTANNVPMNELDLQEIPDLWDPFRSNTISDLEQMFHYTINDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY79</accession>
    <name>A0A1E3NY79_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_34794</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037221.1">
        <property type="nucleotide sequence ID" value="XM_019182232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY79"/>
    <dbReference type="STRING" id="683960.A0A1E3NY79"/>
    <dbReference type="GeneID" id="30199478"/>
    <dbReference type="OrthoDB" id="2158884at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040020">
        <property type="term" value="P:regulation of meiotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="35"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="383" mass="44334" checksum="C900F20C147918E3" modified="2017-01-18" version="1" fragment="single">MGNASDSPRKQKQKIPPDVDNPPFYLPLKPFNGRYRVVSNLGNGSFGSVGLAKSSQDLLDSSKFRIYRNTLLDRNEFYKHENYFNKKTGLVAIKTMTKKLNALNDYSKVKEIRFILAIPYHPNLVQVFELFIDNVSFKLHIVMECLDQNLYQLMKARKNSFFSQTTLKSILSQILNGIRHIHRNQYFHRDIKPENILVSPSNRFYDQEFINSKNFKYHDCFIVKIADYGLARHVDNKRPYTSYVSTRWYRAPEILLRKNWYSRPVDIWAFGSVAAEIATFRPLFPGSDEIEQTWKVLDVLGTPISNNNIGNYFPCYGYWDEAAVLCSQLGLKLPVKECIDITHLIPNRDLTPLCDVIKACLAWNPNTRADVEAICAMPYFKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY95</accession>
    <name>A0A1E3NY95_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor SLU7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_101933</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SLU7 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036879.1">
        <property type="nucleotide sequence ID" value="XM_019180336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY95"/>
    <dbReference type="STRING" id="683960.A0A1E3NY95"/>
    <dbReference type="GeneID" id="30197582"/>
    <dbReference type="OrthoDB" id="249612at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030628">
        <property type="term" value="F:pre-mRNA 3'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021715">
        <property type="entry name" value="Slu7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039974">
        <property type="entry name" value="Splicing_factor_SLU7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12942:SF2">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SLU7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12942">
        <property type="entry name" value="STEP II SPLICING FACTOR SLU7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11708">
        <property type="entry name" value="Slu7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor SLU7" evidence="9">
        <location>
            <begin position="110"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="60"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="287"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="353" mass="41371" checksum="FD6CF072E56A4C24" modified="2017-01-18" version="1">MSKENPYVPKYIKDAPWYQPKTNPEDYLSHHRTSSTQEPNSEPKIGQGIKDEFIPTEEIDPTSQQRHRIKKKGRCKNCGGDHDKKDCLERPGLINVKQTGKVSKRNEDVDYDGKRDRWYGYDVNDYTKYLKDWKEKEAKKEPEEKREYDTDEEIELKELGLLDDPEFKDAAKQDGEKIIRLREDKAVYLTNVRNENISYDPKSRMIRDDKTGYYNDSNLFVRHLTGEAKEFENSKKFAWDEKKRGIIDDNLVANPTLADRKIEKMSEEEKLKKDKLKKSILDRYGGSLSISNNDEAEEEEDEDPQAQVDSTKSKYPEDVYHNNHTSVWGSYYSDGKWGYGCCKSLYKNSYCSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYD2</accession>
    <name>A0A1E3NYD2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_95592</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037383.1">
        <property type="nucleotide sequence ID" value="XM_019186499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYD2"/>
    <dbReference type="GeneID" id="30203745"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="59" mass="6750" checksum="1F69485DD590BC3C" modified="2017-01-18" version="1">MPQLLLDIKPYNPHTSTTSNTKKQTNNIPTYLYVKTQLYSSYNTDIVQPTTTTDNINTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYG6</accession>
    <name>A0A1E3NYG6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_85301</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>myo-inositol(out) + H(+)(out) = myo-inositol(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:60364"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037423.1">
        <property type="nucleotide sequence ID" value="XM_019185872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYG6"/>
    <dbReference type="STRING" id="683960.A0A1E3NYG6"/>
    <dbReference type="GeneID" id="30203118"/>
    <dbReference type="OrthoDB" id="6339427at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005366">
        <property type="term" value="F:myo-inositol:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904679">
        <property type="term" value="P:myo-inositol import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17360">
        <property type="entry name" value="MFS_HMIT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000073">
        <property type="entry name" value="MFS myo-inositol transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="121"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="400"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="436"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="470"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="46"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="540" mass="58945" checksum="47FBDC8EEE2AB203" modified="2017-01-18" version="1">MSLFNDKKDFKTVKTDFQQKHSDQEDDTSILMTFHNTKPSPLIIVLTIISSISGFMFGYDTGYISSALVAIGTDLDNKVLTYGEKEMITAATSLGALITATMAGLSADFFGRKPTIMSSNILFLVGAALQCAAHTFWVMTVGRFIMGFGIGIGSLVSPLFISEISPSRFRGRLTVINCLCITGGQLVAYGCGAGLTHVTHGWRILVGLSLIPAAIQFFVFFWLPDTPRYYIMKGQLEKACAVLKRTHLDSTEELIELKVQELSRLNNQIPGSNILIQTWNAIKEVHRVPSNLRALIIACGLQGIQQFTGFNSLMYFSSTIFETVGFKDSTAVSIVVAGTNFVFTVIAFFIIDWAGRRLMLLLAIPGMIVSLVVCAIAFHFLGVKFDGGEAIVESSGISGWGIVVIIGMILFVATYAIGIGNVPWQQSELFPQSVRGIGTSFATATNWAGSLVISSTFLTMLRNITPPGTFALFAALCAVSWVFVWFCYPELSGLELEETQQLLTGGFNIKASKILAKKRKSHIDVENQIKASHEIVNHVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYJ9</accession>
    <name>A0A1E3NYJ9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Manganese/iron superoxide dismutase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_33543</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037351.1">
        <property type="nucleotide sequence ID" value="XM_019182085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYJ9"/>
    <dbReference type="STRING" id="683960.A0A1E3NYJ9"/>
    <dbReference type="GeneID" id="30199331"/>
    <dbReference type="OrthoDB" id="275227at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S26, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="2">
        <location>
            <begin position="148"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="2">
        <location>
            <begin position="252"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="329" mass="36920" checksum="48D58F67689F9B2D" modified="2017-01-18" version="1">MLSLRSKALQASRRFNSTSSVFKYYKTPEIPQIDVNAANGINGLFSKQGLQNAWYKRAEIYCDALDKEIAREGLAAHDPEVLVNQYAKSSSKLQLFNNASLLYNTEFAMSCLGGVRNEDSLPAKPDSKSLLSNPDISHEIPNQPIDETFQDWIKDSFGSVLEFKTLLLNSANAINGDGFTWLVARKTRKSLASQSSATKEEYDNLYVLNTYNAGTPNNSVRFGQLTQISEKLRKLSEQSEDKSMTFKPPASSILSLVDARNVAVYGNTEYKPILAIDASPKAYLYDYGVYGKKTYLEQVWKALDWDIITRRLPPRSENYTVEGRIGRGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYK3</accession>
    <name>A0A1E3NYK3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase</fullName>
            <ecNumber evidence="3 12">2.4.1.131</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_85868</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes, on the cytoplasmic face of the endoplasmic reticulum, the addition of the fourth and fifth mannose residues to the dolichol-linked oligosaccharide chain, to produce Man(5)GlcNAc(2)-PP-dolichol core oligosaccharide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>an alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + 2 GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + 2 GDP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:29523"/>
            <dbReference type="Rhea" id="RHEA-COMP:19515"/>
            <dbReference type="Rhea" id="RHEA-COMP:19516"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:132511"/>
            <dbReference type="ChEBI" id="CHEBI:132515"/>
            <dbReference type="EC" id="2.4.1.131"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11 12">
            <dbReference type="Rhea" id="RHEA:29524"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.131" evidence="3 12"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036989.1">
        <property type="nucleotide sequence ID" value="XM_019185962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYK3"/>
    <dbReference type="STRING" id="683960.A0A1E3NYK3"/>
    <dbReference type="GeneID" id="30203208"/>
    <dbReference type="OrthoDB" id="2276068at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004377">
        <property type="term" value="F:GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03806">
        <property type="entry name" value="GT4_ALG11-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038013">
        <property type="entry name" value="ALG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031814">
        <property type="entry name" value="ALG11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919:SF1">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15924">
        <property type="entry name" value="ALG11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="ALG11 mannosyltransferase N-terminal" evidence="14">
        <location>
            <begin position="10"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="13">
        <location>
            <begin position="236"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF15924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ57782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="483" mass="55641" checksum="2AF0CA2179EC7D5A" modified="2017-01-18" version="1">MSTKELPKGKVYGFFHPYCNAGGGGERVLWAAVQSTLDKNPNDKIVIYTGDVDSTSQEILENVVKRFDIKLDNSRVEFKFLQKRYLVDPKTWPFLTLLGQAFGSLVLAYEAARLYKPDIWVETMGYPFTYPLIHYLLGVPIVAYVHYPVISKDMLGKLDISLTSIFGLKQLLKYFYWSIFMFIYTFVGSYVDITLTNSTWTYNHIKNIWFLNENISIVYPPCSTEKFIESKTPTHWDRKNIIVCIAQFRPEKRHGLIIEEYKRFLDNSHYSSHPPKLVLIGSIRGQADNDYVEELKLKANKLEIPEKNIEFILDAKYEVIKEYLRASSFGLNAMWNEHFGIAVVEYVASGLIPIVHASAGPLLDIVVPWDSQLKKQVKDHSIKNRTGFFFKSETDPDYSNFKDKSLYPSLSEAFLEATNLTDEEKLEITSRGKECVLNKFSDATFDKDWITKKQKPSHLLRNSLIFALILGFLKSIVFNSQNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYX1</accession>
    <name>A0A1E3NYX1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cell differentiation protein rcd1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_34351</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT9 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037485.1">
        <property type="nucleotide sequence ID" value="XM_019182176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYX1"/>
    <dbReference type="STRING" id="683960.A0A1E3NYX1"/>
    <dbReference type="GeneID" id="30199422"/>
    <dbReference type="OrthoDB" id="1183224at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000160">
        <property type="entry name" value="Cell differentiation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007216">
        <property type="entry name" value="CNOT9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12262">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04078">
        <property type="entry name" value="Rcd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="329" mass="36993" checksum="8F46DE35E54DA758" modified="2017-01-18" version="1">MFNPQVQQQQQQIPPQQVGDIGAQQQQQQSQGAKALDDDKVYQWIIELSYGPNREQALLELGKKREQYDDLPLVLWNSFGVMTSLLDEIVSVYPLLSPPNLSSAASNRVCNALALLQCVAAHPETRNLFLNAHIPLFLYPFLNTNSKQRSFEYLRLTSLGVIGALVKNDTPEVISFLLTTEIIPLCLRIMESSSELSKTVAIFIVQKILVDDAGLAYVCQTYERFAAVASVLKIMVDQLATQQTTRLLKHVVRCYLRLSDNPDARKALRQCLPESLKDATFHAVLRDDVASKKCLAQLLLNLGDYEGQQAQSLSQQQQQQQVPLQQQRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZJ6</accession>
    <name>A0A1E3NZJ6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">PRP1 splicing factor N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_32292</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037826.1">
        <property type="nucleotide sequence ID" value="XM_019181939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZJ6"/>
    <dbReference type="STRING" id="683960.A0A1E3NZJ6"/>
    <dbReference type="GeneID" id="30199185"/>
    <dbReference type="OrthoDB" id="440128at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071001">
        <property type="term" value="C:U4/U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000256">
        <property type="entry name" value="Probable pre-mRNA splicing factor prp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055433">
        <property type="entry name" value="HAT_Syf1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010491">
        <property type="entry name" value="PRP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF1">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23233">
        <property type="entry name" value="HAT_Syf1_CNRKL1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06424">
        <property type="entry name" value="PRP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="PRP1 splicing factor N-terminal" evidence="9">
        <location>
            <begin position="11"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats" evidence="10">
        <location>
            <begin position="697"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="30"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="868" mass="100335" checksum="2166A1673A145E37" modified="2017-01-18" version="1">MERKAFLDQEAPVGYIAGIGRGATGFTTRADVGNARLPPQQRQIGSDDEDNFQDVDESGLLGDSLVNKDDEEADAIYQDIDNRLKNRNKRRLSPDGEDKAEDNNLTKIANQFADLKKKLEAVTEDEWANLPDAGDFTKRNKRHRKLLQEQKRSYAAPDSLISGLNASGNIDSSVDLSALTSERQKLLGSKIDSNFDFNEDDGVDSATYLNEISNLSQNNDEEIKKIKTILNSFTKADPKKPEGWIARARLEEFNKSFEAAKKLIQQGCTNCPFDEEIWLENVRLNRSDVKYAKIIVAEGVKLNNKSLKLWLKATELEQEVFNKKRVLRKALESLPTSPELWKESIKIEENVEDQIRIAEKATELAPEDEELWLELISLQPYEIAKSTLNKARKALPNNVSIWLTACKLELVNNPGSDKIEKMIKKTFKECNLTKDEWYKHAIEFENQQLSLLTDLIVNELLSQDLEPDYSSLVSEADKYKDQIFVYKSILNFIVIKFPKKTAIWRRLIELYKLNFEASELYKIFENIIEILPKNPLFWLMYSKEVWKNGDIPSAKQILQRAFKFLDSNADMWLALIKLESVEQNYDQVRELFKEARARVTNERIWYKYVTFLRQSDATNEALELIEDGLMNFPSCFKLYMQKAQILEESGDLVKARNVLSVGTKSVPDSVELWIYLSDIDFKLDNGTKARSDLDLGLLKNPQSDKLWFKKLQLELKLKNIDQIKVILTKALKEFPHSSYLWSFNLTFNTKKSLRKTLYQDALNATNNDVRVLIIIGYNFWIDGKLDKAKRWFERAIIANEDFGDAWGWYYNFLIKNNEPTEEFDAFLKKFSDVEPHHGDVWPIVSKRIENLDKQPTDILKLVAQELIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZL6</accession>
    <name>A0A1E3NZL6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor CWC21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_80601</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC21 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037671.1">
        <property type="nucleotide sequence ID" value="XM_019185091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZL6"/>
    <dbReference type="STRING" id="683960.A0A1E3NZL6"/>
    <dbReference type="GeneID" id="30202337"/>
    <dbReference type="OrthoDB" id="10267305at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd21372">
        <property type="entry name" value="cwf21_CWC21-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051372">
        <property type="entry name" value="CWC21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013170">
        <property type="entry name" value="mRNA_splic_Cwf21_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36562">
        <property type="entry name" value="SERINE/ARGININE REPETITIVE MATRIX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36562:SF5">
        <property type="entry name" value="SERINE_ARGININE REPETITIVE MATRIX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08312">
        <property type="entry name" value="cwf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01115">
        <property type="entry name" value="cwf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="CWF21" evidence="10">
        <location>
            <begin position="62"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="11"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="119"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="133" mass="15653" checksum="03A76CE41EB15A7C" modified="2017-01-18" version="1">MSYNGIGLSTARGSGTNGFIQKNYTRSNNETSYSKRLKNKQNDAKRDALINNSDLIKDKELVKHDEKRSIELKVSEYRDKLEEEDEDLDDDEIDAKCKEYKEELIKEFNIKQGYKSRRSREDSRDTKQQDVDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZQ7</accession>
    <name>A0A1E3NZQ7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_32549</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the UTP23/FCF1 family. FCF1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037886.1">
        <property type="nucleotide sequence ID" value="XM_019181977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZQ7"/>
    <dbReference type="STRING" id="683960.A0A1E3NZQ7"/>
    <dbReference type="GeneID" id="30199223"/>
    <dbReference type="OrthoDB" id="76105at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09864">
        <property type="entry name" value="PIN_Fcf1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000004">
        <property type="entry name" value="rRNA-processing protein FCF1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037503">
        <property type="entry name" value="Fcf1_PIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="PIN" evidence="6">
        <location>
            <begin position="59"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="185" mass="21267" checksum="FFE1AFCED37B66BD" modified="2017-01-18" version="1">MGKAKKTRKFAATKRIITNKDQRMYKQTPKKDDPELARHVPQVSSALFFQFNEAIKPPYQVLIDTNFINFSIQKKIDIVRGMMDSLYAKCIPLITDCVMAELEKLGPKYRIALRLAKDPRIQRLTCSHKGTYADDCLVQRVLDHKCFIVATNDADLKRRIRKVPGIPIMSVGSHSYVIERLPDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZV3</accession>
    <name>A0A1E3NZV3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Multicopper oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_85545</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037751.1">
        <property type="nucleotide sequence ID" value="XM_019185908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZV3"/>
    <dbReference type="STRING" id="683960.A0A1E3NZV3"/>
    <dbReference type="GeneID" id="30203154"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13850">
        <property type="entry name" value="CuRO_1_Abr2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF488">
        <property type="entry name" value="LACCASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0186">Copper</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0410">Iron transport</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133584" description="Multicopper oxidase" evidence="9">
        <location>
            <begin position="20"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="12">
        <location>
            <begin position="47"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="10">
        <location>
            <begin position="191"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="11">
        <location>
            <begin position="504"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ58544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="657" mass="73670" checksum="6E85215CA2CA88EC" modified="2017-01-18" version="1">MLTSFVLTSLIFLVNLVNSLPLDITFINPEDVIPQGANKIYYDWNVTVQTQNVEGLYRQLFLINGQSPGPTIEGDQGDWVVLKVKNFSPVPITIHFHGIHQKDTQFSDGTAGVTQWPILSGDSFVYVINLKQYGLYWYHNHYRGYANDGLVGPIYIRPNSSVSRPYNEITSNEDDLKLLNSLEKQPQLLLFSDLFKETYDAVASKMFDFGMDPTCVRSFLTNGKARHQCYDKQLLQNEAFATKTSKLQKMLKTNDTIHVDDYGCLDLQKMNGFTSIDYDKDLLEFPGFNNNDCKNSTTDREVLYTKNQSYIMLNLLNFGGEFSKMLSIDDHNITVIAVDGTFVKPITGQQVFLPIGQRVTAIVETDPSKHENIKTPFAIRTACHDMPQIIEGLALLMYVEKNGKQDIQKIDYPSNGEMYQNRGAELLSKAFKLVDPRYIPPFDQSLKPPSGPADKTIYLYLNRLGATSFSIVDNKTMSGGMELDYPSLYLIANDSTRSVNLSETISPAFIDQGISKGDVVDIIYQNSPIADHPMHLHGHTFWTIGYNDSASVFPYKSVEEALNDGRADLFDFEQPSYVDNLSITTGGYAITRMIADNPGAWMIHCHIQTHLAAGMGAVLISAKEDIPDLPSSLFNQAHVDYNSMVSQSPSELNVTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZZ3</accession>
    <name>A0A1E3NZZ3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Presequence protease, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Pitrilysin metalloproteinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_32025</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Degrades mitochondrial transit peptides after their cleavage in the intermembrane space or in the matrix, and presequence peptides; clearance of these peptides is required to keep the presequence processing machinery running. Preferentially cleaves the N-terminal side of paired basic amino acid residues. Also degrades other unstructured peptides. May function as an ATP-dependent peptidase as opposed to a metalloendopeptidase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer and homodimer; homodimerization is induced by binding of the substrate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion intermembrane space</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M16 family. PreP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037988.1">
        <property type="nucleotide sequence ID" value="XM_019181911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZZ3"/>
    <dbReference type="STRING" id="683960.A0A1E3NZZ3"/>
    <dbReference type="GeneID" id="30199157"/>
    <dbReference type="OrthoDB" id="10250783at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000013">
        <property type="entry name" value="Mitochondrial presequence protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000009">
        <property type="entry name" value="Presequence protease, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000011">
        <property type="entry name" value="Presequence protease, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013578">
        <property type="entry name" value="Peptidase_M16C_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055130">
        <property type="entry name" value="PreP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016">
        <property type="entry name" value="PRESEQUENCE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016:SF13">
        <property type="entry name" value="PRESEQUENCE PROTEASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08367">
        <property type="entry name" value="M16C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22516">
        <property type="entry name" value="PreP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01264">
        <property type="entry name" value="M16C_associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16C associated" evidence="16">
        <location>
            <begin position="484"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ58781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="990" mass="111900" checksum="37CD008E80CCF445" modified="2017-01-18" version="1">MLRTLQKGNFAKGVRGLASYNQAKSLRRYPVGQTLHGFEIKRVLPVPELSLTAVDFVHNRTGSNHLHIDRDDPNNVFSVGFKTNPPNKTGVPHILEHTTLCGSHKYPVRDPFFKMLNRSLANFMNAMTGHDYTFFPFSTTNDKDFENLRKVYLDATFNPLLKKEDFYQEGWRLENEVVDDKSSPLTFKGVVYNEMKGQESNSSYLFWIKFQEALYPSLKNSGGAPSKITDLTYEDLLDFHAEHYHPSNAKTFTYGSLPIVETLEALNKEYIAFGKRNIKNIVKTPVELKKSTNVIVEGPVDPMAPPEKQLKASITWPCGNPSDVYETFCLKILSNLLTDGHSSPLYKALIETGLGDDFSVNSGMESMTASNFFTIGLHGLQSIEELENEVIKVLEKHSEEGFEDSKIEAIIQQLELGKKDQKSDFGMSLLYSILPGWVNKVDPFDVLAWDDIISQFRIDYKKGDLFQNLIKKYFLNKPVLRFQMKATETFDESVKNEEVERLQNKIQDLDQEDRDVIFERGRHLAKKQGEVEDLSSLPTLRVRDIPRTSTVKPVSFSEVENSKVQRRITDTNGLTYFRASRDVNIPYELYPYLTLFSDALTNLGTESQSMADIEDQIKLFTGGLSSSVSVHSSPIDLSPRVSFNFNGVALNQNSSHIYDIWQNLLLNTNFNNKEKLATLIRLLSSNNISGVAESGHSFARNYAGSSVSRAKAISESLNGIEQLQFLNKLNTWIQDDEHFQKNIIDKLNELKQYLVNSQDLRFSLISDEASVLENEKLISSFVSKLPSESIPQTPVKDFPLNPSHKSFIKLPFQVSYASSVLQGVPYVHEDGARLQVLSNLLTFKYLHREIREKGGAYGGGAAYSGLDGLFSYYSYRDPKSLQSLETFQKAGQFAVDNEWSECDLEEAKLTIFQSIDAPISVKSEGQVLFKEGVTDEMRQTRREQLLDVDITDIKEVAEKYLVNGKPSIALVGDSQGDQKDWNVVDLGVQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZZ5</accession>
    <name>A0A1E3NZZ5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Band 7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_31888</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037865.1">
        <property type="nucleotide sequence ID" value="XM_019181895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZZ5"/>
    <dbReference type="STRING" id="683960.A0A1E3NZZ5"/>
    <dbReference type="GeneID" id="30199141"/>
    <dbReference type="OrthoDB" id="2105077at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000004">
        <property type="entry name" value="Putative membrane protease family, stomatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264:SF19">
        <property type="entry name" value="AT06885P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="BAND 7 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="3">
        <location>
            <begin position="95"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="372" mass="41427" checksum="94B7D65F5AC92C30" modified="2017-01-18" version="1">MSQEFYDAPKAQPGYTDDTPSGETFQQNIINNNPSATSNTIRNPPIVSREPDAIKQITYKKEYDVQPQGTYQSLVNAFGNFFGYFGMVCCCPCSNPYHDVNQGSIGMITKFGELARVADPGSTYVNIFSEKLHMVSIMIQVQDIPEQACLTKDNVIVYLKSVLYYNIIDPQKAVFGISNIQDAIVERTQTTLRDVIGSRVLQDVIERREEIAEAIQIIIAQTAAEWGVHVESILIKDLQLSNEVTQSLSQAAQAKRIGESKIITAKAEVESAKLMRQAADILASKPAMQIRYLDAMQNMAKSANSKVIFMPSSGELDRNASNVTTENGEHSDQIEEVDEEKLRQLQAQLDQRHGASQTDNYKNMIAVKESLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P004</accession>
    <name>A0A1E3P004_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 9">Uracil-DNA glycosylase</fullName>
            <shortName evidence="7">UDG</shortName>
            <ecNumber evidence="7 9">3.2.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">UNG1</name>
        <name evidence="11" type="ORF">WICANDRAFT_84523</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.</text>
            <dbReference type="EC" id="3.2.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 9">Belongs to the uracil-DNA glycosylase (UDG) superfamily. UNG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.27" evidence="7 9"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037998.1">
        <property type="nucleotide sequence ID" value="XM_019185723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P004"/>
    <dbReference type="STRING" id="683960.A0A1E3P004"/>
    <dbReference type="GeneID" id="30202969"/>
    <dbReference type="OrthoDB" id="10031947at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097510">
        <property type="term" value="P:base-excision repair, AP site formation via deaminated base removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10027">
        <property type="entry name" value="UDG-F1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.470.10:FF:000007">
        <property type="entry name" value="Uracil-DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00148">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002043">
        <property type="entry name" value="UDG_fam1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018085">
        <property type="entry name" value="Ura-DNA_Glyclase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003588">
        <property type="entry name" value="PRK05254.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003589">
        <property type="entry name" value="PRK05254.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003592">
        <property type="entry name" value="PRK05254.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00628">
        <property type="entry name" value="ung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264">
        <property type="entry name" value="URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264:SF0">
        <property type="entry name" value="URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00987">
        <property type="entry name" value="UreE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00130">
        <property type="entry name" value="U_DNA_GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="10">
        <location>
            <begin position="108"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7 8">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="296" mass="33918" checksum="AE1D756404E13641" modified="2017-01-18" version="1">MLNEKRVFDAAVNSASVTETTETRANEKLEINIPEPSDFKKQFITKLTDEQKELLDLEINTLEDSWFQALSKEFLKPYFLDLKKFLKTQYNAKQTIFPPQNEIYSWSRLTPLNQVKAIILGQDPYHNFNQAHGLAFSVKDPTPPPPSLKNMYKGLKIDYPDFVIPTGSSAGDLTKWAARGVLLLNTCLTVKAHEANSHSKKGWEQFTEVVLKKAIENDKKIVLFLWGMPAQKRVVNMKLNPEKIHVLKSVHPSPLSARRGYFEGHHYIQCNKWLMENGEQPIDWALADGNTIDYTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P015</accession>
    <name>A0A1E3P015_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dolichyl-phosphate beta-glucosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.117</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_64421</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-alpha-D-glucose = a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate + UDP</text>
            <dbReference type="Rhea" id="RHEA:15401"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.117"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15402"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.117" evidence="4"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P015"/>
    <dbReference type="STRING" id="683960.A0A1E3P015"/>
    <dbReference type="GeneID" id="30200977"/>
    <dbReference type="OrthoDB" id="3784at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004581">
        <property type="term" value="F:dolichyl-phosphate beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04188">
        <property type="entry name" value="DPG_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035518">
        <property type="entry name" value="DPG_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="14">
        <location>
            <begin position="114"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ58282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="360" mass="40024" checksum="F9DCC91E55C7AFC6" modified="2017-01-18" version="1">MVIISTGIALTLASGLLTLLFLARGDLPYAIGAGVAALTTLLIHILDYYYAIAIISTGIVLVYSTIVLFSHNPRSPHESELHFITTNTETSRDSTTYPLPKRGITTQIEEIELSVVIPCYNETKRLKIMLEDAISYLRTTYDTKFEILIVDDGSKDGTAEYALDLAFNYFELYPGQLRVVKLVKNRGKGGAVTHGIQHVRGKYAVFADADGASQFSDVSKLIKAAESIKGPAVAVGSRAHMVNSDAVVKRSFIRNFLMYSLHTLVFVFGIRDIKDTQCGFKLFNKEAIVEIFPYMHTEGWIFDVEILIIALRKQIPIKEVAISWHEVDGSKVDLAKDSINMAIDLVVTRLAYIFGIYKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P083</accession>
    <name>A0A1E3P083_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_85347</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019185879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P083"/>
    <dbReference type="GeneID" id="30203125"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="59" mass="6825" checksum="E279E13228498221" modified="2017-01-18" version="1">MPKLSTTFCSGATLLPKEAITGMFHARCWHWSPWQSTYRQTLKTDEQVSLSQALAKYKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0B2</accession>
    <name>A0A1E3P0B2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_79251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019184863.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="30202109"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="34544" checksum="4F51E0B16D8A0309" modified="2017-01-18" version="1">MLLLQLFNIAMLANLVIQNNQIFDLFKNQFLNSSKLRRLYKSYFLLSIITFSGFTFLNIQSKDYFKLLFTIPLLITSTIYIIRCFITRIDPFDQYENQLLITSAIQSPSQINSTGPVAIIRTSQGDFKSEDVSPILSTNNTTTHIGSLTKKDNNLSAPILATLLGAETVMARPLMEPSTSSTTIPILAILISISFIFSQWCYITAIFKSNVYTPLRQYLVTALINLIISTTLIIFTEFQLNDIAMIQFGQFNMGLKICNYLISISLLLQSVSTIMMLMMSRKHEKEALQVVEVYNSIPSPRSISY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0I4</accession>
    <name>A0A1E3P0I4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_79374</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038035.1">
        <property type="nucleotide sequence ID" value="XM_019184879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0I4"/>
    <dbReference type="GeneID" id="30202125"/>
    <dbReference type="OrthoDB" id="3982939at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133612" description="RING-type domain-containing protein" evidence="4">
        <location>
            <begin position="19"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="274"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="332" mass="38613" checksum="00A8207B23F64C0D" modified="2017-01-18" version="1">MKTTKLIFLWLLALHVNAVPLPNAESRTPYEFHLGGSETPVNQTALHSPHTATIIDKDTKLPHGSDELDSNNGTLTLSTHPESYPHHDTSTRNSPPKHYTEKSPYLILIPICFILGLFWAILSYWPLKYSYNLLIKKWCDIELGDYSKVSTNFRKVFCFQFFLLVIVVIIFMIPSLPWFIAGGIGFAVQEFFHWLARRNNDRFDRERAIQQGAAKETKKYTRVTRTDVDEYVPHFDTLLAQSYKPTSKSRSFNDFTKFGKLKSEKPDLVSEDFCVLCLDAYSDDSEVLLLPCEHYFHFKCFRRMGIMPQELIIFKYMISHRCTLMDTNNDIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0K1</accession>
    <name>A0A1E3P0K1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Protein ROT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_85471</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for normal levels of the cell wall 1,6-beta-glucan. Involved in a protein folding machinery chaperoning proteins acting in various physiological processes including cell wall synthesis and lysis of autophagic bodies.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ROT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037644.1">
        <property type="nucleotide sequence ID" value="XM_019185895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0K1"/>
    <dbReference type="STRING" id="683960.A0A1E3P0K1"/>
    <dbReference type="GeneID" id="30203141"/>
    <dbReference type="OrthoDB" id="5327821at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006458">
        <property type="term" value="P:'de novo' protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007118">
        <property type="term" value="P:budding cell apical bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030950">
        <property type="term" value="P:establishment or maintenance of actin cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019623">
        <property type="entry name" value="Rot1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28090">
        <property type="entry name" value="PROTEIN ROT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28090:SF1">
        <property type="entry name" value="PROTEIN ROT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10681">
        <property type="entry name" value="Rot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017290">
        <property type="entry name" value="ROT1_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="237" mass="26715" checksum="CF97E9E352BE24FD" modified="2017-01-18" version="1">MQILNSLLVLSAADDSENSIVGTWSSKSNTVFTGPGFFDPVDELLIEPSLPGVSYSFTEDGHFEEALYRITSNPKNHSCPSAVLIYQHGTYETLDNGTIVLTPIDIDGRQLLSQPCKDGGASTYSRYSQKTTFIKHAVIVDDYHGRYRLNLYEWDGTPVQPLYLAYRPPLMLPTETLNPTDNADTRPTGQSLKRRVRRSLENKNRTKAIRKSTFDHDFWWYASIGLVSVGSLGYFFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0U8</accession>
    <name>A0A1E3P0U8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glycine--tRNA ligase</fullName>
            <ecNumber evidence="4">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Diadenosine tetraphosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_32313</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 ATP + H(+) = P(1),P(4)-bis(5'-adenosyl) tetraphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:34935"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58141"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="4"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038041.1">
        <property type="nucleotide sequence ID" value="XM_019181942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0U8"/>
    <dbReference type="STRING" id="683960.A0A1E3P0U8"/>
    <dbReference type="GeneID" id="30199188"/>
    <dbReference type="OrthoDB" id="57698at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070150">
        <property type="term" value="P:mitochondrial glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00774">
        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00858">
        <property type="entry name" value="GlyRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.200:FF:000001">
        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000004">
        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000158">
        <property type="entry name" value="Glycyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.230:FF:000002">
        <property type="entry name" value="Glycyl-tRNA synthetase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000010">
        <property type="entry name" value="Glycyl-tRNA synthetase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033731">
        <property type="entry name" value="GlyRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003211">
        <property type="entry name" value="PRK04173.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745:SF0">
        <property type="entry name" value="GLYCINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="GLYCYL-TRNA SYNTHETASE/DNA POLYMERASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="15">
        <location>
            <begin position="176"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ58834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="658" mass="75111" checksum="A2300C9E85D3E862" modified="2017-01-18" version="1">MSGKPSQVEFSRENLENVLKRRFFFAPAFEIYGGVSGLYDYGPPGCAFQANIIDIWRKHFILEEDMLEVDTTMLTPYEVLKTSGHVDKFSDWMCRDLKTGEIFRADHLVEEVLESRLKGDKEARGLVDNANADAQEDADKKKRKKKVKEIKAVKLDDEVVQEYESVLAKIDGYSGEELGQLMVKYNIGNPVTGETLEAPKAFNLMFETAIGPSGQLKGYLRPETAQGQFLNFNKLYDFNNLKMPFASAAIGKSFRNEISPRAGLLRVREFLMAEIEHFVDPENKKHENFSEVADVKLRFLPRTVQQEGLTTPIETTIGEAVKTGLVDNETLGYFIARIYQFLMKIGVDPERLRFRQHLANEMAHYAADCWDAELHTSYGWIECVGCADRSAYDLTVHANKTKEKLVVRQKLDTPVTVTKYELDLEKKKFGPKFRKNAPLVEDWLLSRNQDELVKLKEELSSNGKITFKIPEIEDQIELDTTFITIEQRTKVEHVREYTPNVIEPSFGIGRIIYSIFEHRFWSRPEATERAVLSFPPIVAPTKVLLVPLSNNEDLQPIVKQISKTLRKEQIPFKVDDSGASIGKRYARNDELGTPFGITIDFESVKDGSVTLRERDSTKQVRGDVKDIIKAIREVTYNGVSWEEGTKNLTPFETKVDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1D0</accession>
    <name>A0A1E3P1D0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_32297</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038345.1">
        <property type="nucleotide sequence ID" value="XM_019181940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1D0"/>
    <dbReference type="STRING" id="683960.A0A1E3P1D0"/>
    <dbReference type="GeneID" id="30199186"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05474">
        <property type="entry name" value="SAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000023">
        <property type="entry name" value="Aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033876">
        <property type="entry name" value="SAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133633" description="Peptidase A1 domain-containing protein" evidence="10">
        <location>
            <begin position="19"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="11">
        <location>
            <begin position="70"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="105"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="506"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="105"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="140"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="506"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="525"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="391"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ59138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="580" mass="61217" checksum="48FBDCACA3FADE61" modified="2017-01-18" version="1">MKLSLLVGIASVSTSVIAKNIAPGVHGFNFDVTRGDTYEDSHKDKRTRLVKRASPDGTVETTLENQRSFYSVEIGIGDHDENVTVLMDTGSSDLWVTGSNNPFCSSGSSSSSGSSSSGSSHRRPVINNAEQDDTNKDSDFGSTQPFGTASQTGRETQTQVSSSQATIDCSEYGTFDVDSSSTFHSNDTEFYIVYGDSSYAYGTWGYDDVTVGGTKVGNLSFAVANQTNSTVGVLGIGLEGLETTYSGSTAANGGGYTYSNFPAKLVDDGLIHKRVYSLFLDSSNARSGTILFGGVDHAKYNGSLLTVPLVNTLASRGYDQIIKLEITLSGVGLQDVSSANIDERTITTTKYPALLDSGTTLTYLPSDLIRSIANALGAEYSRSTGYYITSCFSGSNSGYLVYDFQGKLIRVPLSDIVLQTTSSSSCVLGLFSSGDNSIILGDSFLRSAYVVYDLDDLEISLAQAQYSDEEDIEVVTSTIPSASRIADYSNTWSQYQSATNSGNIFTNTDDSEAAATSNGGDTPRSSQNASGGSSRSSSSSRASSSSSGSNQRSSSASTLNISIVSVFLMSFFVGTGLACL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1I8</accession>
    <name>A0A1E3P1I8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_33033</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038410.1">
        <property type="nucleotide sequence ID" value="XM_019182026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1I8"/>
    <dbReference type="STRING" id="683960.A0A1E3P1I8"/>
    <dbReference type="GeneID" id="30199272"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005619">
        <property type="term" value="C:ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000007">
        <property type="entry name" value="Subtilisin-like serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF13">
        <property type="entry name" value="SUBTILASE-TYPE PROTEINASE RRT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="8">
        <location>
            <begin position="127"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="364"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="380"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="436"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="448" mass="48289" checksum="49346A51C2DF043B" modified="2017-01-18" version="1">MKISTLFFPTLVISGYVIQFKDSLGLKQLLDQDDKSNVLHHIRPYITNKLTIGSFQGIAGNFTDDVVKRLKSSPFVKDIVPDVIVKAFESEQEINSELTIHLARLSRNAKLPSNQAVNYYHEPVFNGKGVFVYIIDTGINKEHPEFEGRAIFGADFTSEGDGDRNNHGTHVAGVVGSKTYGVAKEATLVEVKALDRLGQGSLSTVLAALEFAVNDRKSRGVPGVVNLSLGSAKNTVLNQAIKAAVESGLVVAAAAGNANIDSCLTSPASSSFAITVGAIDDKTDSIAHFSNWGNCVDVFASGVLVNSVDAFNYDNPLVHSGTSMASPSVSGLSAILLQKGVPVNEISNQIKLLSTKDQINRRSKMFRPSTPDRIANNGVQKHDDVYPEEKIEEGNIKDDDSFDSDRLRKFKDGEPRPDDLKSDDSAPQSVGGLDIAVDKHNDESDSTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1S2</accession>
    <name>A0A1E3P1S2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_79402</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038064.1">
        <property type="nucleotide sequence ID" value="XM_019184884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1S2"/>
    <dbReference type="STRING" id="683960.A0A1E3P1S2"/>
    <dbReference type="GeneID" id="30202130"/>
    <dbReference type="OrthoDB" id="10251727at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="43054" checksum="04A7989224CE1129" modified="2017-01-18" version="1">MARKVTNKTKVVKKPQAARKPKGKVVKALNVEDVSPDAKEEEPKVAVSKSVKSLVDDKKISKALSELKKFVANSTKEEESKAQLFEDELSNDLQIIFTKKNLFTSKKNFKPKLIHIQDKPQNEEQPKIVLFVRDDVIDKELLEKIEESELNSLIEQIITGSDLKSTYKQYEKRRELYSLNDIFLADDSLITTLPKLLGKVFYDSKKLPIPIKINKENFSIQSVINQVNKISNSIVYSLPRSNNLSVNLGSIETINESVLKQVIEHFQNEELRSIFIKTNQSPALPLYELDTVYTEDDVKPTDKEEIKAKESNVSIEGLPENIKLSEFEKGLIELANPTEIPELFSSKIKKSIKKSKQENKDKVSKPLLKKKTKKSIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1S7</accession>
    <name>A0A1E3P1S7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial DNA replication protein YHM2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_63933</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038646.1">
        <property type="nucleotide sequence ID" value="XM_019183642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1S7"/>
    <dbReference type="STRING" id="683960.A0A1E3P1S7"/>
    <dbReference type="GeneID" id="30200888"/>
    <dbReference type="OrthoDB" id="10253709at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005371">
        <property type="term" value="F:tricarboxylate secondary active transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015742">
        <property type="term" value="P:alpha-ketoglutarate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006843">
        <property type="term" value="P:mitochondrial citrate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000053">
        <property type="entry name" value="Mitochondrial DNA replication protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000085">
        <property type="entry name" value="Tricarboxylate carrier, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053017">
        <property type="entry name" value="Mito_Cit/Oxoglu_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46982">
        <property type="entry name" value="CITRATE/OXOGLUTARATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46982:SF1">
        <property type="entry name" value="CITRATE_OXOGLUTARATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="14"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="207"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="308" mass="33001" checksum="17724445A7912205" modified="2017-01-18" version="1">MAPNQELKKKPVSFSNILLGAGLNLAEVTTLGQPLEVIKTTMAANRSLSILQSAKLVWSRGGVLGFYQGLIPWAWIEASTKGAVLLFVSAEAEYQFKKIGASNFGSGIGGGIIGGLAQAYLTMGFCTCMKTVEITRSKQAAVGGVAQQTSFQVFKEIYAKEGLRGINKGVNAVAIRQCTNWGSRFGLSRLAEDVIKNLTGKNKDEKLGGLEKILASAIGGGLSAWNQPIEVIRVEMQSKTNDPNRPKNLSVAGAFKYIYQNNGIKGLYRGVAPRIGLGIWQTIFMVGFGDMARELVGKLTGETPVAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P295</accession>
    <name>A0A1E3P295_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Letm1 RBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_31866</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038531.1">
        <property type="nucleotide sequence ID" value="XM_019181891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P295"/>
    <dbReference type="STRING" id="683960.A0A1E3P295"/>
    <dbReference type="GeneID" id="30199137"/>
    <dbReference type="OrthoDB" id="275278at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033122">
        <property type="entry name" value="LETM1-like_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044202">
        <property type="entry name" value="LETM1/MDM38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009">
        <property type="entry name" value="LEUCINE ZIPPER-EF-HAND CONTAINING TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009:SF1">
        <property type="entry name" value="MITOCHONDRIAL PROTON_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07766">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51758">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Letm1 RBD" evidence="10">
        <location>
            <begin position="160"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="408"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="419"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="429" mass="49465" checksum="83B6190956F23D63" modified="2017-01-18" version="1">MIRQIIGSQSRKIIPSQSIYSRPLIYRTIHSSYPKFQQDVKPTKEAPPSTPAKPVEKKKTLWEKVKHEANHYWDGTKLLGLETKISFKLLLKSTAGYELTRREHKQLKRTTQDVVRLVPFAMFVLVPFAELLLPVALKLFPNMLPSTYESKLDKEKKMKRLRKTREVVSEVLRDTVRVKLPSYVTEDQKADFREFFRLVRSGTESPSREQLLRVARLFKDDIVLDNLQRAQLVAVSKYINTSPIGTNQMLRFRIRYKLLKIKQDDRAIDYEGIDSLSTVELQAACASRGIKVNGVPPHELKDDLSIWLHLRLREKIPSTLLILSNAYTYGDIESKESLYDALESVLSAIPDELYHEAEADVAKDDVTNKQRLELIKEQEELIKTETKQEDESGTIIQVKDKINLDDADKTQHLTPAQIEAKKVEAEKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2K8</accession>
    <name>A0A1E3P2K8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">40S ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_104775</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038923.1">
        <property type="nucleotide sequence ID" value="XM_019180429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2K8"/>
    <dbReference type="STRING" id="683960.A0A1E3P2K8"/>
    <dbReference type="GeneID" id="30197675"/>
    <dbReference type="OrthoDB" id="10258210at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.860.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_uS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_uS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005713">
        <property type="entry name" value="Ribosomal_uS19_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003121">
        <property type="entry name" value="PRK04038.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01025">
        <property type="entry name" value="uS19_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="Ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ59716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="16323" checksum="6A1BB181D7ED11CF" modified="2017-01-18" version="1">MSDAATDSRRKRTFKAFSYRGVDLKDLLELPTEEFAKLAHARVRRRFQRGLNNKPYGLIKKLRAAKLATEPNEKPAVVKTHLRNMIIVPEMIGSVVGVYNGKVFNQVEIKPEMVGHYLGEFSITYTPVRHGRAGVGSNRFIPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2P4</accession>
    <name>A0A1E3P2P4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">5'-AMP-activated protein kinase subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_83822</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Adenine nucleotides-binding subunit gamma of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (SNF1) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 5'-AMP-activated protein kinase gamma subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038963.1">
        <property type="nucleotide sequence ID" value="XM_019185605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2P4"/>
    <dbReference type="STRING" id="683960.A0A1E3P2P4"/>
    <dbReference type="GeneID" id="30202851"/>
    <dbReference type="OrthoDB" id="286637at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005641">
        <property type="term" value="C:nuclear envelope lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004679">
        <property type="term" value="F:AMP-activated protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045722">
        <property type="term" value="P:positive regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000217">
        <property type="term" value="P:regulation of invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04618">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04641">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000033">
        <property type="entry name" value="Nuclear protein SNF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF35">
        <property type="entry name" value="LD22662P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9 15" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CBS" evidence="16">
        <location>
            <begin position="179"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="16">
        <location>
            <begin position="246"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ59756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="307" mass="34457" checksum="EBD06E84391CBEAE" modified="2017-01-18" version="1">MAVQAIRQFLKSKTSYDVLPVSYRLVVLDTSLLVKKSLNILLQNNIVSAPLWDAKTSRFAGLLNSQDFINVIQYYFQNPDKFEFVDRLTLNGLKDVEKAIGVEPIDTGSIHPFKPLYEACVKMIEARSRRIPLIDEDEDTHREIVVSVLTQYRILKFVSLNCKETAMLLQPLKDLGIGTNKQISTATMNTPVIDVIQLLSHGSVSSIPIVDESNKLINVYEAVDVLGLIKGGIYNDLSLSVGEALMRRSDDFEGVYTCTVNDKLSTIMNNIRKSRLHRLFVVDDDGKLVGVVTLSDILNYILFGEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2R4</accession>
    <name>A0A1E3P2R4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_53246</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038976.1">
        <property type="nucleotide sequence ID" value="XM_019182709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2R4"/>
    <dbReference type="STRING" id="683960.A0A1E3P2R4"/>
    <dbReference type="GeneID" id="30199955"/>
    <dbReference type="OrthoDB" id="2117459at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06869">
        <property type="entry name" value="PX_UP2_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047168">
        <property type="entry name" value="LEC1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024554">
        <property type="entry name" value="LEC1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024555">
        <property type="entry name" value="PX-associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47185">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN YPR097W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47185:SF1">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN YPR097W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12825">
        <property type="entry name" value="DUF3818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12828">
        <property type="entry name" value="PXB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="3">
        <location>
            <begin position="207"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="290"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="869"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="815"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="296"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="315"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="869"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="879"/>
            <end position="888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="913" mass="106806" checksum="B31E770F113BC8D0" modified="2017-01-18" version="1">MADITPTREHFLKRELLRLQLAKEFEQFNDAYALRRFGYPFTKSDPREYKVEKKLKNGDVKQPDPDDTLDAFPLSKYFFNNFVLTIPFLSSKHQTTEEFWIKKVQVFYEHFMNLSMSESFDRDELTKRKKLGLKLTRVILMFYNSGIGTTNEAIYYEKDKEEVGETIKGSKLDKLMFPSKETLQDHIAQGTFINGINVNVAGVRLVKKANNKFWSNLNFTKSHETYYEFLIKSRVELDESYDICVARRYHDFKELSHALKKRYPGKQLPKLPSKIKSEINVETPVLNQNPVFAEAPKSPKSPKSTKLFKSPTKNLFRSPSTSEKKTQSSNPDASTDNNKLPREKMRVSLRAYLRELLKDDEVSHCDLLKEFLFKGQIFKLSNEEEIDIKIRENLDLLLVLNQVKFQTEAYAKISALREQTMPLRVKLLESDNGIIEVFDELKTKEHITELSPMLRNFIDWCKINIAATIYQLFLGNDNSFEFYSQVRRFHKMLPYSVMINILKFTNPMSIMKTMLDLMMASPFGGKSLLQTLFYGILTDDIKAQLKTINELEVKIHHEDILRRLKYFVYDCEDVDLLESIKQESKELNADLLLTIIITPKLSEYSDVDDEIVGTVFESYKEYKKMKAADDADEKLLINHEKAELYSNLKAVFKLYVKNHDKDILKQIWAEPELTSILKDLLTMFYQPLVNLFKNAHVDVAFKNFENFMDELIITIDRLTNEIYVSDTSKIVDEIMRVLDTHEDEFYQFLHDVYITDSGQIFQQLIEWINSILNFLRNSKNINVSSSNIHKKLDISEIVSNSSNSPEIIKEIDSIIEQVKLQRQQHMEKLSKQQKQKQDLITDNWDAINNIEIFNTGDFGLNEHDVLELNDESESEDEDNDHKIKIKPREEMKTDEIEKLVPIFKDSVVDILKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P326</accession>
    <name>A0A1E3P326_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuolar protein sorting-associated protein 51 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_62472</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039098.1">
        <property type="nucleotide sequence ID" value="XM_019183377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P326"/>
    <dbReference type="STRING" id="683960.A0A1E3P326"/>
    <dbReference type="GeneID" id="30200623"/>
    <dbReference type="OrthoDB" id="203678at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="60"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="31328" checksum="BBEA08638F0AD0F1" modified="2017-01-18" version="1">MSTPGSPVIPQGGFAHHTRTPSTTSSLSEHQISYKKPVKIKNELNEKRRKALKEYYKLKKQEKELKESQGPKERKDSVHDIDSDGEVTEGHPVKEIDFLNSDFKDLVKDTNTLTSSINLINSSIKNIIYNNYYELIKLNEFLKDLNELDLSKLIIQDEDNKTNKKDALNLLNDTMDNASVKSIKLDGGDGFTKLKSLMSEIEELDKKEIKSNTELSNNKVAENITSYLKIEKKEDISEAKDEIKSNMEKLIAQVKGQEDKETLLRQLEEIQKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P347</accession>
    <name>A0A1E3P347_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_78540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039118.1">
        <property type="nucleotide sequence ID" value="XM_019184745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P347"/>
    <dbReference type="GeneID" id="30201991"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133630" evidence="1">
        <location>
            <begin position="20"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="140" mass="15036" checksum="DFC8DC2729955801" modified="2017-01-18" version="1">MFKQYIFILFAAFAHLILAASPNTSGTINGDTANYTISYDPGAIEDSATFSFRGRLYTNSDVEFSDVKFTSSLTKDFVDIGGTGVTGTWKVSQPEDLVGIYIFAKTSDGSDFPEGVDFSVIGGYVLDNGTQSTFSQSITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P391</accession>
    <name>A0A1E3P391_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="8">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_68455</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 13">
            <text>D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the class-I DAHP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="8"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039092.1">
        <property type="nucleotide sequence ID" value="XM_019184119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P391"/>
    <dbReference type="STRING" id="683960.A0A1E3P391"/>
    <dbReference type="GeneID" id="30201365"/>
    <dbReference type="OrthoDB" id="4699125at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000005">
        <property type="entry name" value="Phospho-2-dehydro-3-deoxyheptonate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006219">
        <property type="entry name" value="DAHP_synth_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00034">
        <property type="entry name" value="aroFGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009395">
        <property type="entry name" value="PRK12755.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE DAHP SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225:SF12">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE, TYROSINE-INHIBITED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001361">
        <property type="entry name" value="DAHP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP synthetase I/KDSA" evidence="10">
        <location>
            <begin position="56"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR001361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="365" mass="39699" checksum="372D4B85FAE458CA" modified="2017-01-18" version="1">MSEVPVKPSITPAPEEMEDLNIVGYDPLMSPALLQSDVPASKFSLQTAVKGRKEAAKIIQQQDDRLLVIVGPCSIHDADAALDYAQRLKKLSEELKDELVIIMRAYLEKPRTTVGWKGLINDPELDSSFNINKGLKISRKLFCDLTNQGIPIASEMLDTISPQFLTDLISFGAIGARTTESQLHRELASGLSFPIGFKNGTDGTLDVALDAVQAAAAPHHFMGVTKHGIAAITTTRGNENCFVILRGGKKGTNYDEQSVQEAKAKLPEKGVLMVDCSHGNSNKDYRNQPKVSSEVARQVSKGEDKLIGVMIESHINEGKQSIPKEGKKGLKYGVSVTDGCVGWETTVDMLKELADSVKARRALKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3Y9</accession>
    <name>A0A1E3P3Y9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aspartyl aminopeptidase</fullName>
            <ecNumber evidence="4">3.4.11.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_62698</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal aspartate or glutamate from a peptide, with a preference for aspartate.</text>
            <dbReference type="EC" id="3.4.11.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the peptidase M18 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.21" evidence="4"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039342.1">
        <property type="nucleotide sequence ID" value="XM_019183421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3Y9"/>
    <dbReference type="STRING" id="683960.A0A1E3P3Y9"/>
    <dbReference type="GeneID" id="30200667"/>
    <dbReference type="OrthoDB" id="9880441at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05658">
        <property type="entry name" value="M18_DAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.250.10:FF:000001">
        <property type="entry name" value="Aspartyl aminopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.250.10">
        <property type="entry name" value="Aminopeptidase i, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001948">
        <property type="entry name" value="Peptidase_M18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023358">
        <property type="entry name" value="Peptidase_M18_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002759">
        <property type="entry name" value="PRK02813.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570:SF3">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02127">
        <property type="entry name" value="Peptidase_M18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00932">
        <property type="entry name" value="AMINO1PTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101821">
        <property type="entry name" value="Aminopeptidase/glucanase lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6 11" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ60135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="488" mass="53194" checksum="C52AC9C2955C8F9F" modified="2017-01-18" version="1">MSSTTLKYANDFVSFLNASPTPYHAVHNVKAKLTKNGFQELSERDNWPGKVLKGGKYFVTRNASSIIAFTVGEKWAPGNGISIVGAHTDSPCLRIKPVSKRNAEGFIQIGVELYGGGIWHSWFDSDLSVAGRVIVKQDGLFVPKLIDIKKPLLKIPTLAIHLDRDVNKTFEFNKESQLLPIAGLDNAIPEDKKVKSKGGCCEGSELSTEEFQSLNTVVQRHDEQLVELIAKEAGVTVEDIEDFELVLYDHKPATLGGLNDEFIFSARLDNLTSCFTSIEGLVESVTTESLKDENGIRLVSLFDHEEIGSSSAQGADSNFLPNILDRITGLTGDKGESTKPLPFTLESSAKSFFLSSDVAHGIHPNYTGKYESKHKPKLGEGPVIKVNANQRYVTNSPGIVLLKEVAKKAKVPLQLFVVANGSPCGSTIGPILASKTGIRTLDLGNGVLSMHSIRETSSTVDIEKQVALFKSFYDNFTKIEPTIIIDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4R8</accession>
    <name>A0A1E3P4R8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_68896</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039612.1">
        <property type="nucleotide sequence ID" value="XM_019184144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4R8"/>
    <dbReference type="STRING" id="683960.A0A1E3P4R8"/>
    <dbReference type="GeneID" id="30201390"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000065">
        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF15">
        <property type="entry name" value="TRANSPORTER SEO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="276"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="351"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="501"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="585" mass="67243" checksum="E1CFDC8B0E73CB55" modified="2017-01-18" version="1">MSSISDEKSAPREITESSKWGWTQKLPWFIPHERIVIETQEEIDAGYAIRKKNEEEKQSYHVKEYRDEAHRPWYAFFNEVEYRLTKEEQSKHDWWRWYDKGTSKAEKKLILKQDLLITITAFLGFWTFSLNQANLNNAYVSGMKEEIGMKGNDLINTQVLFSAASIIFQLPLIYILPRVNPTYMLFGAEFLWSIFTLATSSVHNVATLKAFRFIVGAGEAAFFPIIHSNFALNYTHGEIHRRAGFYYFGLYLGTLTSGLLQSGIHNALDGVNGISGWRWMFIIDGIISFGVTFVTLLSIPGSPFKCYSIWLTDDEIKLARKRMRINGTDEHTPTTKEFLDPKVWKKIFTSWHFWVFTIANIGGWNGNSASSGSFVLWLKSLDKYSIGKMNNLTAIPPALGILYIFIVCFGADFIRQRFLMISFSQLMNMIEGAKWFGFCFGYWSWSQSSVFYPLMNDILRRDANQKSIEWLISYIFCAQSNVWLNKILYPTVEAPKFIKGFTAAACFSALQGLFMAIALWFYKRDERAYAYEHGIVLYNSAKGEDPETALKNITQEVKDSDVSSIAISTSSLTEDVVSHHHEPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4R9</accession>
    <name>A0A1E3P4R9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_30258</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039560.1">
        <property type="nucleotide sequence ID" value="XM_019181708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4R9"/>
    <dbReference type="STRING" id="683960.A0A1E3P4R9"/>
    <dbReference type="GeneID" id="30198954"/>
    <dbReference type="OrthoDB" id="296386at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049456">
        <property type="entry name" value="Anoctamin_N_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF73">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20877">
        <property type="entry name" value="Anoctamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="286"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="372"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="507"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="601"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin alpha-beta plait" evidence="7">
        <location>
            <begin position="17"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="6">
        <location>
            <begin position="174"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="653" mass="74373" checksum="2FAF93AEC429B573" modified="2017-01-18" version="1" fragment="single">MPGKILSSNVKPLGELNPDFVLSVKYLVDQTGVPIENSDQKFAKLIKTLKSAGFYSQVRPGEDSTLLLFIKVSDAVLLKALKSFHLKDYLYGVKNSIDDDEQILEHLSSAEKLRAAYSLISDPVFEGGAGITPGAGEWAFVEKIIPLYDYELTKRLITEWSKKPLIDENDIATIKDIAGEKVALYFGFLRFYTVWLVVPSVLGFVSYLFFSRYSIVFTILNLLWAIVMTNAWKKKENVYAITWNTKDSSFIETKRGEFVGETEEIDTVTGIKSPAFPSWKRTLRELAFIPVAIGAALVLVSYQFGCFFIEIFLNEIYDGPGKQFLSLVPTVLIVGFIPILTAGYTIFANKFLAWENHDTNTSYERSSVQKQFILNFLASYMPLFITSFVYLPFGHLVNPYLSFIQTQTSKYNISIQQNRFQINKDRLNQQFAYFAVTAQVVGFFLENIVPVVIRKVTEHFRSSKENNSAVALTDLPEEAQFLSEVRKQVALPPFNVNDEYREMVVQFGYLAIFGPVWSLAPIVSLVNNWIEFRGDAYKLFNETQRPIPVKADTIAPWNTNLRFLTWLATLVAPAITALFRQSDILEESEGSYLKSPVRIPAWTLFAVILFTEHFSIALNFIVEAVYNKIGTKTERNFKSNVYKTRRQYIDGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5B5</accession>
    <name>A0A1E3P5B5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RAVE subunit 2/Rogdi</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_77335</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039863.1">
        <property type="nucleotide sequence ID" value="XM_019184538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5B5"/>
    <dbReference type="STRING" id="683960.A0A1E3P5B5"/>
    <dbReference type="GeneID" id="30201784"/>
    <dbReference type="OrthoDB" id="66510at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043291">
        <property type="term" value="C:RAVE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028241">
        <property type="entry name" value="RAVE2/Rogdi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13618">
        <property type="entry name" value="LEUCINE ZIPPER CONTAINING TRANSCRIPTION FACTOR LZF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13618:SF1">
        <property type="entry name" value="PROTEIN ROGDI HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10259">
        <property type="entry name" value="Rogdi_lz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="36964" checksum="85974AEB83358859" modified="2017-01-18" version="1">MTTEIYPRLEDSKILELEQESKLREQKWLIEHFIKPELPKIIETLGVCLEIITSKDEIKLPISSARTEQVKGIVTRCSSELTDLDIKLHLKSLSKKIHLKLNESIELTQLTEMVGLINQIMSEVQILKDCPADEIKQFVKQLNRILQNLIKCNVVLNKPDEHILFPKHKIQLDQVFEPNGQKLQHYQDRLTIDFFILNSEISIEFKSLEKVTESPWNQIDPSGKSHVDKLREDLKHHKIKLNDILDQDKKKIGHVLGLNRFSTNDYLTRSITFNHNVVIEVEKLLIHCQDPNLISIGAKLTGLEHLVSKMYGNLDMSMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5E7</accession>
    <name>A0A1E3P5E7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_77283</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039810.1">
        <property type="nucleotide sequence ID" value="XM_019184531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5E7"/>
    <dbReference type="STRING" id="683960.A0A1E3P5E7"/>
    <dbReference type="GeneID" id="30201777"/>
    <dbReference type="OrthoDB" id="2152029at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019436">
        <property type="entry name" value="Say1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF31">
        <property type="entry name" value="STERYL ACETYL HYDROLASE MUG81-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10340">
        <property type="entry name" value="Say1_Mug180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="334" mass="38684" checksum="4A42353AC6082956" modified="2017-01-18" version="1">MFTLNYWIQYYTTLPFTLLYNFVVRNKTFNYGLNKLRWIAKTGSYLDVRQNLNEKELKDILALLKKNEFPEISNEEYTSKARWIIKSKNLTKDSPILFFIHGGGFTLKAQKSQIRSTIKTYRALHNEDLSILLIDYQVAPESRFPTPIEEVFYVYHKLTEQDGYKNIIIMGDSAGANLASLVLYQVQNKLENFLKLKVKPNAAVLLSPWVDLAAEKKGSFIKNEQFDYISYEAVRYWGSLYTSEPENPLASPLKLAKWEGALPNKVFYQYGDGEVLLDMIEAFKDKIGVQSKDVHIEPNGTHIHQFVNYSKYSQEKFETTPFCNNLVAFLKTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5H3</accession>
    <name>A0A1E3P5H3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_62646</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039284.1">
        <property type="nucleotide sequence ID" value="XM_019183412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5H3"/>
    <dbReference type="GeneID" id="30200658"/>
    <dbReference type="OrthoDB" id="10553233at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="106"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="183"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="221" mass="26177" checksum="14FBFFB69D27AB9D" modified="2017-01-18" version="1">MDDNKLIRISKKYTNRSIKSWEKPPKHIISSVNKILQMNKTKALQVSASSKKTANLIQQDIDLNEFIKLFLKQLRNTKLPHSHQQRQLNELDYDHDYQLKREAFLTNLYSNELNQVENLKNTLKEENLRDKSITKFVKNYEKLYKEELKKLESIDELPQSSPMNTKRINKSFDTLQLNTGDESEDIAEVLKQLETQLDKIDDNTKHFQELSRQIDQLLNQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5N1</accession>
    <name>A0A1E3P5N1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_28511</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039890.1">
        <property type="nucleotide sequence ID" value="XM_019181497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5N1"/>
    <dbReference type="STRING" id="683960.A0A1E3P5N1"/>
    <dbReference type="GeneID" id="30198743"/>
    <dbReference type="OrthoDB" id="74360at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050982">
        <property type="entry name" value="Auxin_biosynth/cation_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539:SF68">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13738">
        <property type="entry name" value="Pyr_redox_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="640" mass="71821" checksum="6DA90F92DB934398" modified="2017-01-18" version="1">MSVFPGVEEISPDYLETAYDKNIPQEHIYGSLRPDPDNIKHHDVNSLATGWLAEFNNALATIKKDPSQLDKFLLSHTVWKDHLSLQMDLRDFIGPEKIKEILVENIDKIKNFQFDKLADYRSPTGVQLVKIHEEGSNGEVPIEWIQLYLKYETDFGIGRGSIKLVSVDSEDAPIGLKALSIFTILDEFQGIPERVGKKRPQGTIHGENVSRKTWLEKRFEEQDYNGDHQPTAVVVGGGQGGLTIAARLKTFGYNVLIVESNLNIGDNWRKRYKFLSLHDPFYYHKLPYIHSDTSPIYSSKDQFANFLECYAKMLELNIWTNTTVNGASFNDSTKKWEVIVSNNSTNDTRKIYPSHLVFCTGHSGEPNMPSFPNQSNFKGEIVHSSKHTSGANYVGKNALVVGACNSAHDICQDFYEQGVNEVTMLQRSSTCVISSDDGVRVNCEGVFDKDGPDIDTADLLLHGNPIWLTNAIMQQQYRTSSKLDARLLKGLAEKGFKTNAGMGGTGLFGLYYRRGSGYYIDVGCSDLIIQGKVKVKHGTIKKFLEHGVELNDGTILDNLDIVVMATGYSNMKETARKVFGDDVGDKLKPIWGLDEEGEFKSIWRDSGHENFWFHGGNLAMARYYSKRLALQIIAKEEGIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5Z9</accession>
    <name>A0A1E3P5Z9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_62842</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039484.1">
        <property type="nucleotide sequence ID" value="XM_019183449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5Z9"/>
    <dbReference type="GeneID" id="30200695"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="81" mass="9017" checksum="D7E9F6FAD29D6E22" modified="2017-01-18" version="1">MNENKSVMNVSDDESIDMLQSEYECNDNIEEDDSISESESEGENEDEDEGEDKGDDNDGDINHPANVGIQNNVYANGDIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6M2</accession>
    <name>A0A1E3P6M2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CTLH/CRA C-terminal to LisH motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_61498</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040147.1">
        <property type="nucleotide sequence ID" value="XM_019183189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6M2"/>
    <dbReference type="STRING" id="683960.A0A1E3P6M2"/>
    <dbReference type="GeneID" id="30200435"/>
    <dbReference type="OrthoDB" id="3980019at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTLH/CRA C-terminal to LisH motif" evidence="2">
        <location>
            <begin position="20"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="174" mass="20529" checksum="99CF865F3F4012BA" modified="2017-01-18" version="1">MIRNNNEDSKNDDNNQELFVMNIIKFAQDKLTNKAIKNVKFMEELELTMTLLLYSNNKDLLPAKLNELFEFKLRRDIADLVNKSILVKDSNNLIDLNYKKKFYEVKHEDNDNNNGNNEVNNDVDEISIDDNDEDDDGLNSHIIDSNLKKMIKLWIWSENKAFDKNLEYPRLNLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6S2</accession>
    <name>A0A1E3P6S2_WICAA</name>
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        <recommendedName>
            <fullName evidence="9">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="7" type="ORF">WICANDRAFT_67676</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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            <taxon>Wickerhamomyces</taxon>
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    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019184097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6S2"/>
    <dbReference type="STRING" id="683960.A0A1E3P6S2"/>
    <dbReference type="GeneID" id="30201343"/>
    <dbReference type="OrthoDB" id="5588846at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327">
        <property type="entry name" value="RING FINGER PROTEIN 127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327:SF51">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR OF YEAST FORM ADHERENCE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
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    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SPX" evidence="6">
        <location>
            <begin position="1"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="276"/>
            <end position="315"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="44033" checksum="0924E36888EA1355" modified="2017-01-18" version="1">MKFGKTFEKSLEDHHIPEEWIVSSIQYKPLKKKINKVVEEMDDAGLTHEIIAERQLMYYYTFKENQQHEIQPKLLTDDNEDANSINEKMLTLHSDIVFFQALYSQYLKLIQFNKLQSTLILAKIQQLSHLIKKLTSSDQKNKNDMYLWREIFNKYVEYKLDLKSHFDSKNLDSFVGHIEDIKLLKKFKHTKKNTEYFHNFYELNLELLKFLSFENLNTIAIRKIVKKFDKHTLLHSSQNFNKMITFEKSSLSTSSIEQVISTDIVKLVPQLDDYLCPICFSIAYKPVRLTCNHFFCIRCLIKLQRRNEPKCPICRDPVVMDATEANVDYELLEYMKKNFPKEVKKKQSQNEKEVTDETLSTLYGDDKCIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6S8</accession>
    <name>A0A1E3P6S8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rad21/Rec8-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_77703</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040255.1">
        <property type="nucleotide sequence ID" value="XM_019184596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6S8"/>
    <dbReference type="STRING" id="683960.A0A1E3P6S8"/>
    <dbReference type="GeneID" id="30201842"/>
    <dbReference type="OrthoDB" id="3980878at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006910">
        <property type="entry name" value="Rad21_Rec8_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04825">
        <property type="entry name" value="Rad21_Rec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="532" mass="61101" checksum="7082F93CC6EB2CF4" modified="2017-01-18" version="1">MALRLTSNLLYGVALIYKQKTDYLNNDTSLIKTKIQRDLFNQKGSSSDILIKTPNYIIKPIELTKTQQGGNHNNQVLLNDDPLFNLEGDLLPSLDDLGFLQGTSSTQMVNSNHRKSQIKRSDLENNTVSVVLSTMTNSTEDADIFTRHVNHDDILDTQNPEFAFDEEGMLFSLHDETTAGTGGHAGPSEDLHINDGFDIDFDFDIDLERQEAPAQQQPPELDQILEEVEDPPPQQAPDSEQPIVNEPSEAVQQPRKKRRKAQNHFTTLVVDETISLQTNDLRGFRDTYIELMDQQRTKPPEPRITIQNLLDEVSYLPNFGKNIADEERRGRNPVREFEDEDFVDGLLRNTRRSSESIEVRRNASSSRRHSTRASMSSINLPLEGDLQHPQIDDTTGGGGNDNFDFDFDLEFDIDEERVSRKRSSSVQRFPALAEDEEHNYQNNEEMYDDEEPEVDRKTIKFLTFIETRLEEDSVQEMKFDQLMQHQQNRSVIVKSFYELLQLTTLNSIEIVENNDKEKFELLDANDFSIRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6W5</accession>
    <name>A0A1E3P6W5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_28644</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039814.1">
        <property type="nucleotide sequence ID" value="XM_019181517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6W5"/>
    <dbReference type="STRING" id="683960.A0A1E3P6W5"/>
    <dbReference type="GeneID" id="30198763"/>
    <dbReference type="OrthoDB" id="2414723at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31758">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING PROTEIN YLR108C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31758:SF2">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN YLR108C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="28605" checksum="8F00F180E8A80A31" modified="2017-01-18" version="1" fragment="single">MGEFRGFNPTIPDVLPHEKMYNLQIGDKLFVLSGASLSSDAPSYFTNYFLERTEDKPLFIDRSPQSFQYIYNHLQGYHSEIPNGDIFTKVYSDSIYYNLPRLKELLSKDEYYYVEVMNECIKVPKVVLRSEGNFPNFFNITFEALYRDLSELMISRKLIRPPPQAAPQLNRDAGLLKDLIKLLQGVELEMTNDYKKVLRKEARYYRFNALVEKLSYVKIIKNPIILEESIIVNYNDVAYKNFTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6Z9</accession>
    <name>A0A1E3P6Z9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Golgi to ER traffic protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_27975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GET4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040403.1">
        <property type="nucleotide sequence ID" value="XM_019181433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6Z9"/>
    <dbReference type="STRING" id="683960.A0A1E3P6Z9"/>
    <dbReference type="GeneID" id="30198679"/>
    <dbReference type="OrthoDB" id="10252405at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072380">
        <property type="term" value="C:TRC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045048">
        <property type="term" value="P:protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007317">
        <property type="entry name" value="GET4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12875">
        <property type="entry name" value="GOLGI TO ER TRAFFIC PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12875:SF0">
        <property type="entry name" value="GOLGI TO ER TRAFFIC PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04190">
        <property type="entry name" value="GET4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="36088" checksum="740364A10E5AD238" modified="2017-01-18" version="1">MSEVEINVKNPKLQRTLQRFKQKISEGDYYEAHQTLRTIANRNVRTKSYGDAIELLYHAAQILLKSSQPATGSDLTTYLLEIYDEAEIPVDSSSKSKIIQLISLFTPDEPTLKNVSIQAINWTVKFGEYKFGDPYLHDVIGEKFLQNASLAYDAERHLILGTSASFPLYYKLIWTWYLEDPNSKNAGVYVSRLVLNYLFIENIKYANEALSKFIQDLISTKTEFSSETLKESENEVFIFEDLPLINFLQLLLVSVQTKNAPYYQKLKNRYQQDINLAELDDAFSYIGEVYFGIRVQKNINPLQNLMSSFLGGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P757</accession>
    <name>A0A1E3P757_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prefoldin subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_61690</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040327.1">
        <property type="nucleotide sequence ID" value="XM_019183231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P757"/>
    <dbReference type="STRING" id="683960.A0A1E3P757"/>
    <dbReference type="GeneID" id="30200477"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23156">
        <property type="entry name" value="Prefoldin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000001">
        <property type="entry name" value="Prefoldin subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016655">
        <property type="entry name" value="PFD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12409">
        <property type="entry name" value="PREFOLDIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12409:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016396">
        <property type="entry name" value="Prefoldin_subunit_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="129"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR016396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="190" mass="22304" checksum="1156AA68A34E579D" modified="2017-01-18" version="1">MENLLNSKVKNPRGIPQAPFVEKVEDYIKDPADFEKVLSIFQERLQQYKYMEQSKMNAVRQFKIKIPEIKDTLNMVEFLNSKKDSDESIETNYELNDTLYTTAEIQPTDKVLLWLGADVMLEYPIDEAMTLLQDKLSTAQKNLEISEEDAEFLRENITTMEVNTARLYNWDVERRRNIRLAEQGTKKLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P767</accession>
    <name>A0A1E3P767_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_28666</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039914.1">
        <property type="nucleotide sequence ID" value="XM_019181524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P767"/>
    <dbReference type="STRING" id="683960.A0A1E3P767"/>
    <dbReference type="GeneID" id="30198770"/>
    <dbReference type="OrthoDB" id="409725at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034618">
        <property type="term" value="F:arginine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034490">
        <property type="term" value="P:basic amino acid transmembrane import into vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052706">
        <property type="entry name" value="Membrane-Transporter-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43310:SF4">
        <property type="entry name" value="AFR304WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43310">
        <property type="entry name" value="SULFATE TRANSPORTER YBAR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="354"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="405"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="6">
        <location>
            <begin position="475"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="820" mass="92258" checksum="7F9F85836C6B6F3E" modified="2017-01-18" version="1">MKPLKLIGYLPSVILGLLLNILDGLSYGMIIFPIGEKVFSHLGSAGLSMFYVSCIVSQLVYSLGGSAFGAGIGSEMIEVTPFFHGMALSIMHEIGEDQPDKIISTTITTYAISSMITGLIFLMLGKFKLGQLVGFFPRHILIGCIGGVGYFLVITGIEISSRLGAFEYNWGYLSTLLQGENLIKTITPILLTAGLVVLQHYNHNSLILPSYFIAVFVLTHILIILIPSWDLPSSRKHGYMFSSGSNGESSTNEAWYEFYKLYNFKIVEWWIIVKQVPSMLALTFFGILHVPINVPALAVSTKQDNVDVDRELIAHGVSNLLSGAIGSIQNYLVYTNSLLFIRAGADSRIAGIMLAIATFLILIIGPVVIGFIPVCVVGSLIFLLGYELLKEAVWDTWSKVSTVEYLTIVVIVLTMGVYDFVIGILVGIIIASFHFLYENTKIPIIYSQYTGEVARSTVIRHPLQKQFLSKIGKQIYVLKLQSFLFFGTIGKIEKTVRDLFEEENLHKNPIRYLILDFKNVLNVDFSAAEGLNRIKNFIIEQNAYMIISIGDKDNILTSLKNVGLFDHENEAMISKLQVFQDLNASLEWCENEFLTKFKQYQKYKKVEASMSIPTSSSEDNQHLQVNKYAALPINTPRNTNFIKEAQQYIKKESSIIHKPLRSTSTSTNVELQEPLPLLLFSLQGLSNKDESYWKKLVPFFEKRIINADEELHIAPTESFFVLMESGVLTVTYYINSSANIYETLLPRTIIGRFNDDVVRKDRYKSVLKAKANTTIWVLNDTKLNNLKQNKIELYNELLLIVVKLNDERFENITGYTLIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7D9</accession>
    <name>A0A1E3P7D9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein transport protein SEC22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_28683</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040397.1">
        <property type="nucleotide sequence ID" value="XM_019181526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7D9"/>
    <dbReference type="STRING" id="683960.A0A1E3P7D9"/>
    <dbReference type="GeneID" id="30198772"/>
    <dbReference type="OrthoDB" id="1719357at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15866">
        <property type="entry name" value="R-SNARE_SEC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.50:FF:000007">
        <property type="entry name" value="SNARE complex subunit SEC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044565">
        <property type="entry name" value="Sec22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45837">
        <property type="entry name" value="VESICLE-TRAFFICKING PROTEIN SEC22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="16">
        <location>
            <begin position="6"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="17">
        <location>
            <begin position="132"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ61190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="215" mass="24681" checksum="A60A8627B77EFAC7" modified="2017-01-18" version="1">MIKSTLIFRNDGLPLSGSVDDDTDPSLTEQKKKVKLIISRITPNSETRASIESDNYQIHYLMLDSIIYFAICEKSYPRKLAFSYLAEISNEFSQSHGADSLRSDTRPYAFVSFDNYMKKTKKIYQDQRAQSNLDKLNSDLADVKAVMSKNIEDLLYRGDSLDKMSDISSSIRLESKKYRKAAHKINFDLLIQQYAPIVGVGLFIVFLVYWIFLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7M3</accession>
    <name>A0A1E3P7M3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amine oxidase</fullName>
            <ecNumber evidence="9">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_83423</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="9">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <text evidence="8">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="8 9">Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the copper/topaquinone oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="9"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040431.1">
        <property type="nucleotide sequence ID" value="XM_019185541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7M3"/>
    <dbReference type="STRING" id="683960.A0A1E3P7M3"/>
    <dbReference type="GeneID" id="30202787"/>
    <dbReference type="OrthoDB" id="3341590at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009308">
        <property type="term" value="P:amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.20">
        <property type="entry name" value="Copper amine oxidase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000269">
        <property type="entry name" value="Cu_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015798">
        <property type="entry name" value="Cu_amine_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036460">
        <property type="entry name" value="Cu_amine_oxidase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016182">
        <property type="entry name" value="Cu_amine_oxidase_N-reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015328">
        <property type="entry name" value="DUF1965"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638:SF20">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638">
        <property type="entry name" value="COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01179">
        <property type="entry name" value="Cu_amine_oxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09248">
        <property type="entry name" value="DUF1965"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00766">
        <property type="entry name" value="CUDAOXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49998">
        <property type="entry name" value="Amine oxidase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54416">
        <property type="entry name" value="Amine oxidase N-terminal region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0186">Copper</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 7 9" id="KW-0801">TPQ</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133709" description="Amine oxidase" evidence="10">
        <location>
            <begin position="21"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1965" evidence="12">
        <location>
            <begin position="260"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase catalytic" evidence="11">
        <location>
            <begin position="343"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="416"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via topaquinone" evidence="7">
        <location>
            <position position="497"/>
        </location>
    </feature>
    <feature type="modified residue" description="2',4',5'-topaquinone" evidence="8">
        <location>
            <position position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF09248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ61224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="800" mass="91701" checksum="1C34AB7B9E2E57DF" modified="2017-01-18" version="1">MHLLKDIACILAAVTTIVEAASPRQSRPDYYQRLLAKRNPSSFARRDTACSPSFPQEAIAPKKNIWADLDTAEAQIVFDLLNDVYNLTQLENATPYDNYIMWIEALRPNKTDAVAYLDHEGPLPERWARASLFFGAEEHTSEKYPNGFWKELQIGPLNTNATPAIKDLEYLYTKSKVAYEVGYKDKFRWADAQLVFNQVFLSDSMLPVLVDLIGEEMKLYGQEGAPIECWATDPYEYNKETGRTTSWATVFLNYQGAGDITPSGVFLNVDITGRNSSEYFVTKWVYNNIVYNSNEEFRKAWESKSFVKLPKVNSSKMNTTDSFIFVGQQGATRQLDEKLAPISVEPQGRRWKFSEEQRYFTWMDWEFYVAWSRDVGITLYDLKFKGQRIAFEFGLQEAIAEYAGDDPYQAHTTYFDRSYGFGNNAFGLLPGFDCPYNAEMFNVTWHSKGENNYHNNSYCAFEFAEDYPFARHTASDYASVTKNPTFNLRTISTIGNYDYNFLYKFYLDGTFEVSVRAAGYIQGAYYSEETGSPFGYRINQALSGSFHDHVLNFKADFDIAGQKNRVTNTALKATNYTFPWDPENVYSTKIKERSIIEEETYLNWAEAGGVLLIESDEQNNTWGNPKAYRVIYPGGDARRIAQNTKTIGINAEWANHDLTFTKQKDHEVFSSHVLDAQDLQDPIVNFGSYVDGESIDGEDLVCWFNLALHHLPNTQDLPNTIFTTAESSIIFTPFNYFDSEQSRDTLQQLYYKYEDSEWNFHGVDVNPQCELQIPELKFSGISYEASKPINKLNVSLGILY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P858</accession>
    <name>A0A1E3P858_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Catabolic 3-dehydroquinase</fullName>
            <shortName evidence="3">cDHQase</shortName>
            <ecNumber evidence="3">4.2.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">3-dehydroquinate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">DQD1</name>
        <name evidence="7" type="ORF">WICANDRAFT_29546</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Is involved in the catabolism of quinate. Allows the utilization of quinate as carbon source via the beta-ketoadipate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Aromatic compound metabolism; 3,4-dihydroxybenzoate biosynthesis; 3,4-dihydroxybenzoate from 3-dehydroquinate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homododecamer. Adopts a ring-like structure, composed of an arrangement of two hexameric rings stacked on top of one another.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the type-II 3-dehydroquinase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.10" evidence="3"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040264.1">
        <property type="nucleotide sequence ID" value="XM_019181624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P858"/>
    <dbReference type="STRING" id="683960.A0A1E3P858"/>
    <dbReference type="GeneID" id="30198870"/>
    <dbReference type="OrthoDB" id="8191625at2759"/>
    <dbReference type="UniPathway" id="UPA00088">
        <property type="reaction ID" value="UER00178"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046279">
        <property type="term" value="P:3,4-dihydroxybenzoate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019631">
        <property type="term" value="P:quinate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00466">
        <property type="entry name" value="DHQase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.9100">
        <property type="entry name" value="Dehydroquinase, class II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00169">
        <property type="entry name" value="AroQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001874">
        <property type="entry name" value="DHquinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018509">
        <property type="entry name" value="DHquinase_II_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036441">
        <property type="entry name" value="DHquinase_II_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01088">
        <property type="entry name" value="aroQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003804">
        <property type="entry name" value="PRK05395.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003805">
        <property type="entry name" value="PRK05395.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003806">
        <property type="entry name" value="PRK05395.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003807">
        <property type="entry name" value="PRK05395.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21272">
        <property type="entry name" value="CATABOLIC 3-DEHYDROQUINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21272:SF3">
        <property type="entry name" value="CATABOLIC 3-DEHYDROQUINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01220">
        <property type="entry name" value="DHquinase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001399">
        <property type="entry name" value="DHquinase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52304">
        <property type="entry name" value="Type II 3-dehydroquinate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01029">
        <property type="entry name" value="DEHYDROQUINASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 3" id="KW-0672">Quinate metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="3 4">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3 4">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <begin position="105"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="3 6">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="150" mass="16749" checksum="43A29CFCD22A1445" modified="2017-01-18" version="1">MVKKVLLLHGPNLNLLGSREPEKYGTTTLKEIEQNASNQVQAHSSDAQIYFHQDNHEGGLIDRIHQAKADGVEFIIINAGAYTHTSVALRDALLGVDIPFVELHITNVHSREEFRHKSYLVDKAIAIISGLGPIYGYKAAIEFALDYERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8B9</accession>
    <name>A0A1E3P8B9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_75862</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040863.1">
        <property type="nucleotide sequence ID" value="XM_019184275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8B9"/>
    <dbReference type="GeneID" id="30201521"/>
    <dbReference type="OrthoDB" id="69177at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF236">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="120"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="226" mass="25958" checksum="C83CFF7A12388B1A" modified="2017-01-18" version="1">MAKKKVSKTEEIKFTFPNDLRIPSRGFLPTPNVVSEDQIITITKFFSPEFCNELIKSFTTSLKLETTPLIKSKDYAVRVNDRALVESSNTAANLWGYLQKVLLYEYNDESILSEFQTAKGLNPQLRIYRYTKGHHFGQHYDESVRVNNSELKGETKWTLLIYLTGDEEFIGGDTIFYPINKRDDPINVHPSKGTALLHKHGDDCLLHEAQLVRDGVKWVLRSDVVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8E2</accession>
    <name>A0A1E3P8E2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_75877</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040878.1">
        <property type="nucleotide sequence ID" value="XM_019184280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8E2"/>
    <dbReference type="GeneID" id="30201526"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="325" mass="37356" checksum="FCB7824F577B1DA4" modified="2017-01-18" version="1">MSEAELPYCDPVDQNLKSTEGIAPTEDSHNSTVLKDTTNIITPTDPKSIANSNAGEGLTDDKLQHVKVSHWDAEELRYFCQKLAGLSEYKVPNKSRLNMEGRKLLGDYMSQRFPLAAKAKKFEPQKIEHELSLLVDDYINGFLAVKQHSTNSTYSPELNHFVYNTMNPVETEEYKEALTNEILNLTATPHEKRERLKRTRIHRMFHDDVSIYLDVGKFLGIDNNEQYKTTSVNSPPPQQQAGPVNQCDSMNEWIEHLKMNEEKEQAVFLSFLKYLLSEEMITRDDLFKLIMNTKPTDPLMILCTNENIGIDEKLTMLREDLARLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8F6</accession>
    <name>A0A1E3P8F6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_90564</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040439.1">
        <property type="nucleotide sequence ID" value="XM_019186243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8F6"/>
    <dbReference type="STRING" id="683960.A0A1E3P8F6"/>
    <dbReference type="GeneID" id="30203489"/>
    <dbReference type="OrthoDB" id="2399539at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052202">
        <property type="entry name" value="Yeast_MetPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782:SF12">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="53"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="665"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="15"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="143"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="665"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="762"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="781"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ61232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="907" mass="102714" checksum="3A664C98D0A71973" modified="2017-01-18" version="1">MAKQKATVIAESINSPASTTSSIESSAATPASSSSGSAGAFRNPHDKKGRSTSCLLCQKRKQKCDHRLPSCTTCIKAGVKCIQPAKYNVKVPEKDEYTIMLEKKIKFLEKILDANNIVIDAENGTSKKNFKYKKLSPFLGNNPPGSSTNDFPPQSQPTVDNKIIPIPTNRKLPIPTDELSKKSAYLSIDSTDYSNSLFAKYSLKEFLNFDPVFEFDEKLSRAFLDIHFTRLQFKYPLLDEEEIFEFHNAYVTNNLSGYMNNNDSFHYCCCRMWMIFAISSCLHMTTGKYNGPPPDRYFATAIRHITKVSQLNNYQKIEILTLAVLYLIRTDRDSVCLYEIIKDSLKLCVEMGLNKAATYATLNAKERVIKMRLWWCVYLLERMITIAVGKPYSLPENEVDIDLPLFEFEPSKAKHPSNTIFINQSIKLRRIESRFVEKFNIVGKHGSATNDDSQTIHKDQLPLVETFFQELEIWRGNCEGFSNGIENETLRLYYYRSVRLLIQPFLELMDPMDKLFRECQAAAGQICQLFKVFHEKTVFGHSTTAIHTVFVAGVTLVYCLWLARNKDDLKRKALGDVSKHTRPTVSEALFNGLNDLRACSICLYVMAERSKFALIFRDTFDQLMTATITNLIERCGPDSSELIYNSNEPGMPPAIFRRSIHEQNGSLAPTMPHYNSASSDEEKFEQAERKRKQGQLQKNVVPKSLSHLLINSPPLKAETPPNIPVSRTPQSMIPQPQQPALKKQKLDEDLTFSPLSYPGIYNHQSPISQQSQMSGHSAPLSTSSQTSASTQHQQNLLQPQQQQPQQQQQNAQQQQYFPVEFTPFNGRTNTMINNISVWTGESGGPPISTASLFDYNNKNNQRNIILNGSGSVGNHQNGNGSTNLDNYLWNVPVEEFWTNNDDYGFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8F7</accession>
    <name>A0A1E3P8F7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="10">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_25095</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="10"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040896.1">
        <property type="nucleotide sequence ID" value="XM_019181102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8F7"/>
    <dbReference type="STRING" id="683960.A0A1E3P8F7"/>
    <dbReference type="GeneID" id="30198348"/>
    <dbReference type="OrthoDB" id="3264224at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901524">
        <property type="term" value="P:regulation of mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF20">
        <property type="entry name" value="[3-METHYL-2-OXOBUTANOATE DEHYDROGENASE [LIPOAMIDE]] KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Histidine kinase" evidence="11">
        <location>
            <begin position="250"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ61689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="386" mass="43764" checksum="2AA3A4F7E40FDBF2" modified="2017-01-18" version="1">MLKYRSIIRTLSTTSPLKTSHKPSSPRPSLSFEKEYNIRSSLEHLITDYSGKTIPQITLDSLINISKKDISTNAIATINYLTIFNTKRLAAFRALPYLVVLNPHISETYQLYLKSLEILLSIDQSEYKNFDKIHNSLLYYSEIHKDAIPSLSKGFQEVSKFFPKGKIVDFLNLHFYDKINMETITNNYINLINKPENHIGVLNTKLNVSEMLQTWAGLVNDMAFIKYYKQVPIQIDFGQDVEFPYIGIHLEYIFTEILKNSIRANIESGNGDKPIKITIVSNSISSNRKNLSIRFRDDGGGIPPEVEKNVFDYSFTTVEKHEEDKIGMNTNSMPGESVENIAGMGYGLPLTKAYVELFGGNLELQSIYGLGTDVYIELIGPDTSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8N8</accession>
    <name>A0A1E3P8N8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EKC/KEOPS complex subunit BUD32</fullName>
            <ecNumber evidence="6">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24 25">Atypical Serine/threonine protein kinase BUD32</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">EKC/KEOPS complex subunit bud32</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">WICANDRAFT_59854</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the EKC/KEOPS complex composed of at least BUD32, CGI121, GON7, KAE1 and PCC1; the whole complex dimerizes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. BUD32 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="6"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040982.1">
        <property type="nucleotide sequence ID" value="XM_019182887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8N8"/>
    <dbReference type="STRING" id="683960.A0A1E3P8N8"/>
    <dbReference type="GeneID" id="30200133"/>
    <dbReference type="OrthoDB" id="3399at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070525">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000745">
        <property type="entry name" value="Serine/threonine-protein kinase BUD32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022495">
        <property type="entry name" value="Bud32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03724">
        <property type="entry name" value="arch_bud32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209:SF0">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT TP53RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209">
        <property type="entry name" value="NON-SPECIFIC SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06293">
        <property type="entry name" value="Kdo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="21" id="KW-0010">Activator</keyword>
    <keyword evidence="18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="19" id="KW-0779">Telomere</keyword>
    <keyword evidence="22" id="KW-0804">Transcription</keyword>
    <keyword evidence="20" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="15"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00019973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00030980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00033194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="ODQ61775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="267" mass="30277" checksum="94D62B00D0CF8F50" modified="2017-01-18" version="1">MSEEIINKAKSHLDNISVEVISQGAEAVVFTTTQHPYLPTQIKPKLVKHTEKYIIKFRPPKKYRHPILDAQLTKRRTLAEARILQRLTTIPEVHTPSILAVDPRNGILWMEYIGEPLPNGKLSSLKNWLWQYNEDGSSALSSDVKTILESVGREIGSLHLNDLIHGDLTSSNIVLQKSVDNGTLEAFLIDFGLGSVSTVVEDKAVDLYVLERAILSTHPLYSDHYNKWLLEGYSSVYTGKQNSKKLKEVLNRYEDVRMRGRKRSMLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8T6</accession>
    <name>A0A1E3P8T6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Dynamin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_86762</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041018.1">
        <property type="nucleotide sequence ID" value="XM_019186069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8T6"/>
    <dbReference type="STRING" id="683960.A0A1E3P8T6"/>
    <dbReference type="GeneID" id="30203315"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160189">
        <property type="term" value="C:peroxisomal-mitochondrial contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160190">
        <property type="term" value="F:peroxisome-mitochondrion membrane tether activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990627">
        <property type="term" value="P:mitochondrial inner membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990626">
        <property type="term" value="P:mitochondrial outer membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000638">
        <property type="entry name" value="Transmembrane GTPase Fzo1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027094">
        <property type="entry name" value="Mitofusin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465:SF0">
        <property type="entry name" value="SARCALUMENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465">
        <property type="entry name" value="TRANSMEMBRANE GTPASE FZO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="687"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="720"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="Dynamin-type G" evidence="14">
        <location>
            <begin position="208"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="427"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="438"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ61811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="845" mass="95564" checksum="D3DEC699D24B9CAE" modified="2017-01-18" version="1">MSQKYGDLKGKGPEDHEENQDFDSDNKTVFGDQAATLTYLRDEGVLEGSTVFDGDDNHLKVETQHHRSFSNDRHLKTQISQLQYNNNRIALDRSISNTVQILGELQVENKERPLFYPTNDLPNELLNSRKAHLALIRKNSVQTVKSIAKEIADEVENSDSPELKILKLNVKLDNNQISKLDKTAIGQLLDEKIFQVSKHLLSLKDRIDDTSSKVFVTGDLNSGKSTFCNALLRRKVLPEDQQPCTSVFCEVIDARENNGVEEVHAVPIGSTYDIKNESTYKIFQLKDLEELVSECDNYAILKVYVNDRRTIDQSLLRNGVVDIAIIDAPGLNLDSYQTTEVFSRQEEIDLVVFVVSAENHFTLSAKEFIATAANEKKLIFIVVNRFDNIKDKNKCMNRILDQVQRLSPDTHKDAREFVHFVSSSDVVDHLPNDNGEDPGVGGSGDGDGNGAPDDNIHPDFDHLENSLRNFVLQKRALSKLQPAKTYLSKLLNDIEALSTINQKMYFNDRDEMVDKLNEIAPQYEEGLVQSVKVNEKIESLIEKTSQTVYDFTKNNINSTLETVGDHPVVQYKGILNIVEYTVETQRAITKKILDSVSSSEDFARKRTIETIDSIKGLGKSALGEAYNSDKVFREEFMFSRRKDTISRNLDESITFFDFFDPSFESFLTVFGVNEKTKDQAILWKNSFYSIGLYAASGALTTGSIIKGVFQYGSLFSLKTLRFLVLPLSIGVGVVSLTYLIVDIPRALPRKLAKKIKHQVKELDYPHQNSDRIAKECRKVLKYPAREVQNAFQTSLDKHAVKKEKIMKAIKDADVASAFFGKLLKRTMDQRKLVESFDLESISTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P924</accession>
    <name>A0A1E3P924_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sphingoid long-chain base transporter RSB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_82080</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the ATP-dependent translocation of sphingoid long-chain bases (LCBs) from the cytoplasmic site toward the extracytoplasmic side of the membrane (flip-flop). Involved in the establishment of the functional lipid asymmetry of the plasma membrane. Regulates intracellular levels of LCBs, sphingolipid precursors that are growth inhibitory at increased levels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lipid-translocating exporter (LTE) (TC 9.A.26.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041123.1">
        <property type="nucleotide sequence ID" value="XM_019185330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P924"/>
    <dbReference type="STRING" id="683960.A0A1E3P924"/>
    <dbReference type="GeneID" id="30202576"/>
    <dbReference type="OrthoDB" id="3358017at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007568">
        <property type="entry name" value="RTA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465:SF9">
        <property type="entry name" value="SPHINGOID LONG-CHAIN BASE TRANSPORTER RSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04479">
        <property type="entry name" value="RTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ61916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="235" mass="26694" checksum="711E984D125869E9" modified="2017-01-18" version="1">MGGVYILLGKFIMVYGAQFALMKPMAYSYIFMGCDFVSLVVQAAGGAMAASATTKSDNELGTHVMVAGLAFQVFSMTIYVALFLHYFYKIKFFNKKEHEELEVEAFNPAYKQLRSRKIFQWFPYVVFACVALIYIRCVYRVVELAEGWSGYLITHELFLFFLDALMIALTAFLLIVFHPGTVFDGRNIKIQVAGQSKDKKTNHEGVPLDDLKKDGDFDEASDQSFNSVKFKRNHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P943</accession>
    <name>A0A1E3P943_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_62030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040679.1">
        <property type="nucleotide sequence ID" value="XM_019183299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P943"/>
    <dbReference type="STRING" id="683960.A0A1E3P943"/>
    <dbReference type="GeneID" id="30200545"/>
    <dbReference type="OrthoDB" id="5348546at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22699">
        <property type="entry name" value="FHA_PLM2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="124"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="522" mass="58939" checksum="9DB7EC6B7B2ABCD9" modified="2017-01-18" version="1">MSTSGQFPPSSPFLDHHQEEDADPFSSKTSTNVTNQSKKFVINTPFNKDNSKTYPTPNPSSSTGVRSSSPARQNATITNNTEIVQTPDRASSPQESTFLGSENVEYKDPLNDVLRAKITSNHTTTLGRSSRCDYFVKSKFASRVHLKLQYDSERNELSILCKGFNGCVINFGTQIYGYVKQVSNHLFYFQKIEEFEIKSDDEIFHDISLFRGEQITVPYMDSLTLEIKDHVTVLDVCNDDSETEDELPVLNTRFSSVEPGKIEQVERINVTPVESGEVKEVEEKSVKDVEGNPIEKVDEAKAEETESKSIEKTPVEVKPVEVKPIEVKSDKSTQDVKESSQNDFKLATTKKSNQPLQPKSESSLNKIPQSRRRKAEPGISPQKKKPLIKHLQPRDKISQLNIKEIVSTVEDLASVENVLINHLAFSRTSQTPLYTLQTVSAITQNLSRTQLRAVLFQIQSIGTIYRKGKDAAGKPLDEEYYYDVEKDDDLERTKLVSSIKGASGLRNCRKTHKQYFWKKPTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9H2</accession>
    <name>A0A1E3P9H2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription regulator LGE1 helical region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_60111</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041248.1">
        <property type="nucleotide sequence ID" value="XM_019182937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9H2"/>
    <dbReference type="STRING" id="683960.A0A1E3P9H2"/>
    <dbReference type="GeneID" id="30200183"/>
    <dbReference type="OrthoDB" id="4096471at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22897">
        <property type="entry name" value="Lge1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021581">
        <property type="entry name" value="Tscrpt_reg_Lge1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11488">
        <property type="entry name" value="Lge1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator LGE1 helical region" evidence="5">
        <location>
            <begin position="208"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="48"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="89"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="152"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="170"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="292" mass="32877" checksum="9E171E00B4152293" modified="2017-01-18" version="1">MSGRPYSAGSSGNSSYGEPYHRREGYERGGRGRGGYVRESGRGDYRGGYRGSSYRGGRGGGYQSGYHGSYNSQEYQRDSYSSPSAAPRHNSDSFRRDSEPRESEDASASTQEAKRNSLPEVEGYNRSSRDDFRGGYRGGRGSYRGSYRGGYKPRDGYSPYRRESYAHGPSTPNSRANSLNGAHSYHDSPESNNQSPHRVEPEKKSYKSPWIQILKLDAANFEDSELNFKLKEVMEEQEAIYADQEKIENQIKEQKIKNLKIQMELGVLKACAEKDALNVELTQEKLDTISFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9M3</accession>
    <name>A0A1E3P9M3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="9">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_27080</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="9"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041275.1">
        <property type="nucleotide sequence ID" value="XM_019181336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9M3"/>
    <dbReference type="STRING" id="683960.A0A1E3P9M3"/>
    <dbReference type="GeneID" id="30198582"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF103">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MANNOSIDASE MNL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="397"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="458"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="548"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="356"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ62068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="557" mass="64790" checksum="465BD73CC1FC9934" modified="2017-01-18" version="1">MPDDYFKNQKGTNLRKHKKKYLYPVGKENLIKFPKISKKENYSGVQSTYFNDGGRKVQDKLNSIKEAFRSSWSTYKKHGYGHDEVRPISHKASDPFNGWSSTIIDALDTLWIINEKEEFAEAVNFIEKINFKQSFREDIPIFENIIRILGGLIGGYDLSNEQILLNSAVEFADFLIEAFDTPNHMPLLYYRWQDEMPNRLASREACLAEVGSLTLEFSRLTQLTGDNKYYDAVARITDFFQKSVDRFFMKGLVPNKLDISGFFPRQAKQRYSLGGLADSYYEYLPKMYHLLRGDEKHAKVYKDLYLAALNDIKNYMLFMPKIPGGEKILFASSIGVYKNEDKIIVEQELDMQHLTCFAGGMFGLGARVFNRSDDMEIAEKLTMGCVHLYEKLNIMPEVLRDKGVHKEDPLYQIAIPWGNELPPVEVLEDGKTVWTVDETHNQPRWINSMVPEYILRPEAIESVFYMYRLTGDSKWRDYGWMMFENIVKYCKTPEGEFASLRDITVRGARPDNFKDSLESFWFSETLKYFYLLFDDISVLSLDDYVLNTEAHAFKLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9P2</accession>
    <name>A0A1E3P9P2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_82200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041295.1">
        <property type="nucleotide sequence ID" value="XM_019185349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9P2"/>
    <dbReference type="GeneID" id="30202595"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="74" mass="8521" checksum="AB00556C8E34035D" modified="2017-01-18" version="1">MILWDCLKVEKASKILMVLFTVRQGSLLTSCNVQLERILYNHTKGPNTDETIYVELKSEDIFQIVSFIETADAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA10</accession>
    <name>A0A1E3PA10_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_26839</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041438.1">
        <property type="nucleotide sequence ID" value="XM_019181306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA10"/>
    <dbReference type="STRING" id="683960.A0A1E3PA10"/>
    <dbReference type="GeneID" id="30198552"/>
    <dbReference type="OrthoDB" id="277802at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48037">
        <property type="entry name" value="ATPASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48037:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="9"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="102" mass="11682" checksum="26EA8EE5252C87AD" modified="2017-01-18" version="1">MSITNKQLHTLYVSNLNDQVNQSTLKQNLYILFITYGDIIEINMDKKKLRGQAFIAFNNVDSANLAKRSLNGTEFFGKSLKIEVSNQENKKIEELQKSITIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA96</accession>
    <name>A0A1E3PA96_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_24799</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041546.1">
        <property type="nucleotide sequence ID" value="XM_019181058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA96"/>
    <dbReference type="STRING" id="683960.A0A1E3PA96"/>
    <dbReference type="GeneID" id="30198304"/>
    <dbReference type="OrthoDB" id="10266249at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2030">
        <property type="entry name" value="DNA/RNA-binding protein Kin17, conserved domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056767">
        <property type="entry name" value="C2H2-Znf_KIN17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019447">
        <property type="entry name" value="DNA/RNA-bd_Kin17_WH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037321">
        <property type="entry name" value="KIN17-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038254">
        <property type="entry name" value="KIN17_WH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12805:SF0">
        <property type="entry name" value="DNA_RNA-BINDING PROTEIN KIN17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12805">
        <property type="entry name" value="KIN17 KIN, ANTIGENIC DETERMINANT OF RECA PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25095">
        <property type="entry name" value="C2H2-zf_KIN17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10357">
        <property type="entry name" value="WH_KIN17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01253">
        <property type="entry name" value="Kin17_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="250" mass="29169" checksum="51B93E29B04FF1B0" modified="2017-01-18" version="1">MGKAEFGTPKYFSNQMKAKGLQKLKFYCQICEKQCRDDNGFKCHIRSESHLKRIKQVDSHVINDFSKQFQDYFISSLRTYHGEKKINANRFYNQLIQQKDHIHLNSTRWSSLSQFIQDIAKQGIINVKVEDESDQSLNGLDISYINNSSEEIMRKNNLQKHIDNDKSEEQLNMKLLKDQIKRGEQLKKDEEEEQEENKALNRPADDNPIKIKLGGLKKQDNTVNQPKKLNAFKVSKPSKANPLKKNVLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAH3</accession>
    <name>A0A1E3PAH3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_76067</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041084.1">
        <property type="nucleotide sequence ID" value="XM_019184317.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAH3"/>
    <dbReference type="STRING" id="683960.A0A1E3PAH3"/>
    <dbReference type="GeneID" id="30201563"/>
    <dbReference type="OrthoDB" id="10649769at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133757" evidence="2">
        <location>
            <begin position="19"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="319" mass="32205" checksum="EEEEF38A4EE58E4D" modified="2017-01-18" version="1">MLFVKSLAVLALLSLSGAVPVPVPVPVADDSNTFSYDVVNVDGSNAQATSSVQYGDNSNDSSTVGVAEPTSTGTTTATSVSENIVTGTTVVQNPTTTFTIEYTSTTTTTIKGKKKTVWITETEGQNGGQTPTADASTPSSTSTSSTSTSDAPSSADNAQNDVSTTLQQTVIDGTTYLVSAYVSTIRPPSTITYESAYTSELASTGVSTISASTIPVSSSVTQPTSSSVAPSSSASPTSTASTASTTEAPKSSSTSSTSSSTITSAPASSSSIVSEEAYTTVVSDGVCEVYYEDLYDDGSDDSSATTTITSTIITTVSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAJ7</accession>
    <name>A0A1E3PAJ7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_60490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041640.1">
        <property type="nucleotide sequence ID" value="XM_019183001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAJ7"/>
    <dbReference type="GeneID" id="30200247"/>
    <dbReference type="OrthoDB" id="3982631at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="105" mass="11538" checksum="0785B16556891462" modified="2017-01-18" version="1">MLATKQSATILATRRYFSSTFIKALKDSSKASATLINLSSPSSSSVEGGNSFKSFAEYRKSAIQHGPLKKTLINDVGFKPILTDYKDYGVSDIFAKTARSNDYRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAL1</accession>
    <name>A0A1E3PAL1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 13">Adenylosuccinate synthetase</fullName>
            <shortName evidence="11">AMPSase</shortName>
            <shortName evidence="11">AdSS</shortName>
            <ecNumber evidence="11 13">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_76152</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Plays an important role in the de novo pathway of purine nucleotide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11 13">
            <text>IMP + L-aspartate + GTP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11 13">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 13">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="11 13"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041179.1">
        <property type="nucleotide sequence ID" value="XM_019184330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAL1"/>
    <dbReference type="STRING" id="683960.A0A1E3PAL1"/>
    <dbReference type="GeneID" id="30201576"/>
    <dbReference type="OrthoDB" id="10265645at2759"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046040">
        <property type="term" value="P:IMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.170.10:FF:000001">
        <property type="entry name" value="Adenylosuccinate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.300.10:FF:000002">
        <property type="entry name" value="Adenylosuccinate synthetase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033128">
        <property type="entry name" value="Adenylosuccin_syn_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002223">
        <property type="entry name" value="PRK01117.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00184">
        <property type="entry name" value="purA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846:SF0">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00513">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 11 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 11 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11 13" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="11"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="12"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="37"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="39"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="301"/>
            <end position="307"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="333"/>
            <end position="335"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="415"/>
            <end position="417"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ61972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="429" mass="47993" checksum="E72370CEE294E288" modified="2017-01-18" version="1">MADVVLGSQWGDEGKGKLVDILCEDIDVCARCQGGNNAGHTIVVDGVKYDFHMLPSGLVNPKCQNLVGSGVVVHVPSLFEELENLEKKGLHCRDRLFISSRAHLVFDFHQRTDKLKEAELSENKKSIGTTGKGIGPTYSTKASRSGIRVHHLVNDESEAWGEFETRYRRLVTTRMQRYGEFEYDIEEELSRYKKYKELLKPHVVDSIEFMHDALSKDKKILVEGANALMLDIDFGTYPYVTSSSTGIGGVLTGLGIPPKAIKNIYGVVKAYTTRVGEGPFPTEQLNEDGEKLQNIGAEYGVTTGRKRRCGWLDLVVLKYSTLINGYTSLNITKLDVLDTFKDIKVGVSYSLNGHKLNSFPEDLNKLGKVEVEYVTLPGWEQDITQIKKYDELPENAKKYLKFIEDYLGVPIEWVGTGPGRESMLSKPVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAM4</accession>
    <name>A0A1E3PAM4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_76631</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041670.1">
        <property type="nucleotide sequence ID" value="XM_019184423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAM4"/>
    <dbReference type="STRING" id="683960.A0A1E3PAM4"/>
    <dbReference type="GeneID" id="30201669"/>
    <dbReference type="OrthoDB" id="10264538at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000003">
        <property type="entry name" value="60S ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00499">
        <property type="entry name" value="Ribosomal_eL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001380">
        <property type="entry name" value="Ribosomal_eL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018256">
        <property type="entry name" value="Ribosomal_eL13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01294">
        <property type="entry name" value="Ribosomal_L13e"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RIBOSOMAL_L13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="198" mass="22626" checksum="2B34AE4C2D4C102E" modified="2017-01-18" version="1">MAIAKNLPLLKNHFRKHWQERVKVHFDQAGKKASRRQARASKAARIAPRPLDALRPVVRAPTIKYNRKVRAGRGFTLREIKAAGLTPAYARTIGIAVDHRRQNRSTEIFDANVLRLKEYQSKLIVFPRNGKAPEAEQVSTAATFPITQPTPESEPRAVEQQEQTAFRTLRLARSEKKYRGIREKRAKEKAEAEADKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAV8</accession>
    <name>A0A1E3PAV8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Plasma membrane ATPase</fullName>
            <ecNumber evidence="17">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">WICANDRAFT_24948</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Cell membrane</location>
            <topology evidence="2 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="17"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041214.1">
        <property type="nucleotide sequence ID" value="XM_019181078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAV8"/>
    <dbReference type="STRING" id="683960.A0A1E3PAV8"/>
    <dbReference type="GeneID" id="30198324"/>
    <dbReference type="OrthoDB" id="116380at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000011">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000005">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000008">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 17" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="14 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 17" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="306"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="676"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="703"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="739"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="809"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="842"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="53"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="20"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ62007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="902" mass="98344" checksum="C10A3845663D705C" modified="2017-01-18" version="1">MSSEQEHQPTTEKVPKPAPEDDDDEDEDIDQLIEDLQSDAGQEFDEEEAGEAGATARPVPEELLQTDPSYGLTSDEVTKRRKKFGLNQMAEEKENLVVKFLMFFIGPIQFVMEAAAILAAGLEDWVDFGVILGLLFLNAAVGFIQEYQAGSIVEELKKTLANSAIVIRDGSLVEVPSNEIVPGDILQLEDGTVIPADGRIVTEGALLQVDQSALTGESLAVDKHFGDNVFSSSTIKRGEAFMIVTATGDNTFVGRAAALVNKAAGGQGHFTEVLNGIGTILLVLVIVTLLLVWVSSFYRTNKIVRILRYTLAITIVGVPVGLPAVVTTTMAVGAAYLAKKQAIVQKLSAIESLAGVEILCSDKTGTLTKNKLSLHEPYTVEGVDADDLMLTATLAASRKKKGLDAIDKAFLKSLANYPRAKAALTKYKVIEFHPFDPVSKKVTALVESPAGERIICVKGAPLFVLKTVEEDHPIPEEVHEAYENKVAEFASRGFRSLGVARKRGEGHWEILGIMPCMDPPRHDTAKTVNEAKHLGLRIKMLTGDAVGIAKETCRQLGLGTNIYNAERLGLGGAGDMPGSEIADFVENADGFAEVFPQHKYNVVEILQQRGYLVAMTGDGVNDAPSLKKADTGIAVEGATDSARSAADIVFLAPGLSAIIDALKTSRQIFHRMYSYVVYRIALSLHLEIFLGLWIAILNESLNIDLVVFIAIFADVATLTIAYDNAPYAPKPVKWNLPRLWGMSIILGIILAIGTWITLTTMFVPKGGIIQNFGSIDGVLFLQISLTENWLIFITRAAGPFWSSIPSWQLAGAVFIVDIIATMFALFGWWSQNWNDIVTVVRVWIFSFGTFCVMGGAYYLMSESEAFDRFMNGKPIKEKQEQRSLEDFVVAMQRVSTQHEKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB45</accession>
    <name>A0A1E3PB45_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_99043</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041840.1">
        <property type="nucleotide sequence ID" value="XM_019186677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB45"/>
    <dbReference type="STRING" id="683960.A0A1E3PB45"/>
    <dbReference type="GeneID" id="30203923"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000017">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF17">
        <property type="entry name" value="GENERAL AMINO ACID PERMEASE AGP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="379"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="403"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="445"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="483"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="10">
        <location>
            <begin position="53"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="559" mass="61213" checksum="F5B4711A8A61EC3C" modified="2017-01-18" version="1">MSPSNIHDQGSDTSSTSREDKSFKTGPKVTTVPTIEKGEIENVKKKSGLSSRHVALIAISGGLGTGLLVGSGSVLSLGGPAGLLIAYAIVGAMLFATMSAAGELAVAYSSLSGGFNAYAAKLVDPSLGFAVAWSYAVNWFTVLPLEMVTASMTIRFWNSDTNPDIFVAVFLGFVLLINFFGAKGYAEAEFWFNLAKVLMILGFIVLGLIIDTGRAGDIGYVGAKYFINPGPFANGFKGVCACFIASAFSLGGTEFAVLSASSFANPRKAIPTATKQVFYRLLFFYFLSIFVVGLLVPFDSDRLMGSGHNNGAPVSPYVIAIESAGIKVVPHIINAVILIAVLSVGNSAFYSSSRTFYSLAQQGFAPKWFDYVDRNDRPLRAMLVSGIFGLFSFIAAYENQELIFTWLLSLSGLSTIFTWCTICLSHVRFRAALKYQGFSIKELGYVSIAGVYGSYFAIFLDIFILVVHFWVSLFPFHNDGKPSAVSFFQNYSGIFTFLSFYFGHKIYTRNWSFFIKAEEVDLVTDRKIFDPEVLKQENEESKEKLRKGPIFNRVLDFWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB49</accession>
    <name>A0A1E3PB49_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_60701</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041852.1">
        <property type="nucleotide sequence ID" value="XM_019183039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB49"/>
    <dbReference type="GeneID" id="30200285"/>
    <dbReference type="OrthoDB" id="3986620at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="392" mass="45193" checksum="8A16C16346B50C4D" modified="2017-01-18" version="1">MTLDHYSPILSSNLFLQTPVMEQNGETETHPNPYEKISENLEKILTTDSINSKDLYTTYMEIANTARDLIIEFDKIRDDEDLYTVFQLWNMRLTTLLCANELTVAQKEAKRLSAALENVTKSQIASTNSPDQTPPKMLDLFPNEVPLSLKLLIIRLRTQGPNTTLNSEYFKILTDVRMQYTIHENEKSKGSDTPKENLYPVKETFKLGEVLAYAVSGNLIAKREYKTLLTHAESILAPLNKHKSELNESEVLFKDQLSFVSMLVAFITGDLTLGMKYIEDVQNLKDLLETLRYILNNVDPVLDYKKLNTGEKKEDVVQELEILDQVTELVEEQAITGRILCSLCALFELRSRDDQEEKCDAFEKSAKYKYPNMSYQLFKLWFRNTNKLYGFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB74</accession>
    <name>A0A1E3PB74_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_103022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041419.1">
        <property type="nucleotide sequence ID" value="XM_019180368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB74"/>
    <dbReference type="STRING" id="683960.A0A1E3PB74"/>
    <dbReference type="GeneID" id="30197614"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="12"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="575" mass="66984" checksum="9820A1C954131FC3" modified="2017-01-18" version="1">MEDKRPTVDWSLPKELWLKISNDLDMEDSLALANVNRQLRSIFKTKELLIIQAKKRWFSAGDQSIDKPDLGDLWEEFNVFEHCLRRVKIDQYLLQRLQDVTKMTDGLEIFDEMEDIIDHGDAFIPIALKLVDQYEELTLRYYAIQFLMARRHSVAFDTIDEYYEKINHQNDSSLNELPEDLVFKLCHLDPAFDKLLPHRNKVLNEVVKLVKQAELSKESPDYLRSMISLVHSSFIKTVKFHEYAFYDSEEDKSIMRLYAGETAGPTIYQIAIYQKILSLLEIKSELSPGFLIFPGNGQSNQKLYVQMLSLHDIEILTHDEFLGLLSSLHLDALQMNKISSPIQSDFLINAVLAPGETDSLRIQLMEGEVDMNRDEVYPSSRIELPPTFFTVAFHYWMHTPSDLQGIDSLITLTLPRMVFGDMPLTYILIRESSFFENPIDDLNMSIQDAFFYDFEKMNLCDDDSEGSLLEELNINIEFRLGDIVYDNEGTVGVICHAFYNKETSGPLYILYIGNDDFIGVDEVVLNKNPKESEMWMFLEDQKYMGPFFHRYDNKQKRFVPTERLLYESSLSYLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBF9</accession>
    <name>A0A1E3PBF9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">tRNA-splicing endonuclease subunit Sen2</fullName>
            <ecNumber evidence="2 8">4.6.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_60799</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. This subunit may anchor the endonuclease complex to the nuclear membrane. Probably carries the active site for 5'-splice site cleavage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN54.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the tRNA-intron endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.16" evidence="2 8"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041956.1">
        <property type="nucleotide sequence ID" value="XM_019183061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBF9"/>
    <dbReference type="STRING" id="683960.A0A1E3PBF9"/>
    <dbReference type="GeneID" id="30200307"/>
    <dbReference type="OrthoDB" id="10249562at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032473">
        <property type="term" value="C:cytoplasmic side of mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000214">
        <property type="term" value="C:tRNA-intron endonuclease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000379">
        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22363">
        <property type="entry name" value="tRNA-intron_lyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1350.10:FF:000011">
        <property type="entry name" value="tRNA-splicing endonuclease subunit Sen2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006676">
        <property type="entry name" value="tRNA_splic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016589">
        <property type="entry name" value="tRNA_splic_SEN2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00324">
        <property type="entry name" value="endA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227:SF0">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011789">
        <property type="entry name" value="tRNA_splic_SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA intron endonuclease catalytic" evidence="10">
        <location>
            <begin position="231"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00062061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR011789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR011789-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="353" mass="41237" checksum="BBA98799F8962E80" modified="2017-01-18" version="1">MSKSVPLSQLHKYSLPVHPIDLPDLIPHNPISWVLCLLAYWRSSTVVAKTTIHFKDGDFFVTNDEDIKTLWRDGFFGKGIFSRSEATWQDRTQKRLGLGEFKNLTVEEITALRREERKKFKKERSKLEAKQAELRKAGIVDPFIEERLGLKNLRDKDININVKREIDFREEDNDLLEDGKLKNLEQLQLMPVETLFLKFALNAADVICDGSNLSAQQLFNKISSHRADDQFILNYVSYHHYRSLGWCVRSGIKFGTDYLLYKRGPPFHHAEYATIILPNYKDEEENERVAKDFIWLSSISRVIGGVRKNLVLVFVDIPSQEEFDAQPDLESLFKLYKISEVLYRRWVPNKNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBJ5</accession>
    <name>A0A1E3PBJ5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_60727</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="protein sequence ID" value="ODQ62670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_019183046.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A1E3PBJ5"/>
    <dbReference type="STRING" id="683960.A0A1E3PBJ5"/>
    <dbReference type="GeneID" id="30200292"/>
    <dbReference type="OrthoDB" id="10027823at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
    </dbReference>
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        <property type="entry name" value="MFS_dom"/>
    </dbReference>
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        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520:SF5">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
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    </dbReference>
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        <location>
            <begin position="24"/>
            <end position="45"/>
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    </feature>
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            <end position="76"/>
        </location>
    </feature>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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            <end position="171"/>
        </location>
    </feature>
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            <end position="316"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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            <end position="346"/>
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    </feature>
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        <location>
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            <end position="433"/>
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            <end position="440"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="ODQ62670.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
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    </evidence>
    <sequence length="440" mass="48514" checksum="960E5F5DB2783326" modified="2017-01-18" version="1">MVSIAEHIGVNTILSAPRDIHLILIARCTRLAAFGAISLILVQYLKSKGINETEIGILLTFTLVGDLIFSFLLALVSDSLGRRKILLLSSLLITLTGALLYYFENIFIISIVMIFGVVTTSGGEVGPFRSIEQSAISSLTRYEDRSDIFSWYTFTGGFSAGLGSLVIGLLVGKDKYEVCFLFYTINGMILFILNFLLSSEIEIENDITVKASERLTPESETLLGGDSEEIEEPVTPNEKKSLIWKLVPKFSPESLKKLLILTVLICLDSLGMSLSTKAWTAYYFKIRYALSSSSLGLIFGTAAISGALMALVGTYFCKKYGPIRTMVTVHTTASVMLVMMALPIGMREMVTLFLTRSLIRTMDLPSKHVFISATVEPQERTAAIGFINASKTLVNTFGPLISGVLASKDKMWLCFLVAGILRLSYDVGLYINFWDFKDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBN8</accession>
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        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
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        <name evidence="3" type="ORF">WICANDRAFT_60861</name>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042022.1">
        <property type="nucleotide sequence ID" value="XM_019183070.1"/>
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    <dbReference type="GeneID" id="30200316"/>
    <dbReference type="OrthoDB" id="3991675at2759"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ubiquitin-like_dom"/>
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        <location>
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            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="148" mass="16456" checksum="086952A92F6139E4" modified="2017-01-18" version="1">MDEQQEQSITLLFLNTHRLSIPLTLTNNVSILKKYLIENWSADFITPLDSDDSNMGFKPTSIDQIRMIHLGKPLTDDMLISDLNLNLSDIIHVSFKPAVKNEQQQQHKGLSLKRGKSTTSNENKNTNDGSASNSNVQEKNGGGCCVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBR8</accession>
    <name>A0A1E3PBR8_WICAA</name>
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        <recommendedName>
            <fullName evidence="8">CFEM domain-containing protein</fullName>
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    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_60721</name>
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    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
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                <person name="Scheuner C."/>
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                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
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            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183043.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3PBR8"/>
    <dbReference type="GeneID" id="30200289"/>
    <dbReference type="OrthoDB" id="4095829at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008427">
        <property type="entry name" value="Extracellular_membr_CFEM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05730">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00747">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52012">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133782" description="CFEM domain-containing protein" evidence="7">
        <location>
            <begin position="22"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="CFEM" evidence="8">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="144"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="26"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="30"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS52012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="216" mass="20658" checksum="AFCD7BDB379475CD" modified="2017-01-18" version="1">MRSFTISAVAILSLLASEVVATPPACLLACASQVVKKSNKCNTLNQVSCVCENEESAIKQCLDSICPNGQADDAYSSFESSCKDVGAEVKSGSSSSSASSSSSSSASSSASSFASSSSASSSSSSSAAPSSSSVVESSSSAVESSSSSSSVAPSSSAAPSSSAAPTSSAAPASTVSSVISSASSTHAVATQSADAGLILNAPVGALVGAAAFGLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBV7</accession>
    <name>A0A1E3PBV7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_60904</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042065.1">
        <property type="nucleotide sequence ID" value="XM_019183080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBV7"/>
    <dbReference type="GeneID" id="30200326"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="228"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="261" mass="25693" checksum="4A0D8901D92D6D77" modified="2017-01-18" version="1">MKIPIILTFTLSLLASTTFGFPLLRIFARNTDGGYSYSSSLLAAPTNGYNQTNSYQSSAAASPTFGKGYFNTTSKATGGGGSGNTQVHTATVTQIHTLTQTNDAGEAVTTTLTSTIQTQITQSAAANSAQGGGDGNVEAVSTGQDNDDDSGNTANGGSTSATETVMVTATVTLPPPSSLGSNGGAAGSSTGYNYANTTITTSSLSSYALNSTSSLNNNTTSAGGDLNSTSSIATTGASSSAAPLTSLTGVSASSSSVSTAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC57</accession>
    <name>A0A1E3PC57_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">WICANDRAFT_60525</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the DEAD box helicase family. DECD subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041674.1">
        <property type="nucleotide sequence ID" value="XM_019183012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC57"/>
    <dbReference type="STRING" id="683960.A0A1E3PC57"/>
    <dbReference type="GeneID" id="30200258"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000346">
        <property type="term" value="C:transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17950">
        <property type="entry name" value="DEADc_DDX39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000809">
        <property type="entry name" value="ATP-dependent RNA helicase SUB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000111">
        <property type="entry name" value="DEAD-box ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="19">
        <location>
            <begin position="44"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="75"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="262"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="44"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ62467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="428" mass="48434" checksum="B3B158418D4DB43F" modified="2017-01-18" version="1">MSHEGEEELLEYSEPEDNIVQETTAGETKDGDDKKGSYVGIHSTGFKDFLLKPELSRAIVDCGFEHPSEVQQVCIPQSIHGTDVLCQAKSGLGKTAVFVLSTLQQLDPVPGEVSTLVICHTRELAYQIRNEYLRFSKYMPDVKTAVFYGGTDIKKDAELLKNKDTAPHIVVGTPGRLNALLREKYLRLNNLKNFVIDECDKVLEAVDMRRDVQEIFRATPHEKQVMFFSATLSQEIRPICKKFLQNPLEIYVDDEAKLTLHGLQQYYIKLAEREKNRRLADLLDSLEFNQVIIFVKSTARADELNKILTASNFPSIAVHSGIPQEERIARYKSFKDFNKRICVSTDVFGRGIDIERINLAINYDLPNEADQYLHRVGRAGRFGTKGLAVSFVSTEEDETVLGKIQERFDVKIAEFPEGGVDPSTYMNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCE8</accession>
    <name>A0A1E3PCE8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">A1 cistron-splicing factor AAR2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_60610</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAR2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041764.1">
        <property type="nucleotide sequence ID" value="XM_019183024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCE8"/>
    <dbReference type="STRING" id="683960.A0A1E3PCE8"/>
    <dbReference type="GeneID" id="30200270"/>
    <dbReference type="OrthoDB" id="201752at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13778">
        <property type="entry name" value="Aar2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13777">
        <property type="entry name" value="Aar2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.550">
        <property type="entry name" value="Aar2, C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007946">
        <property type="entry name" value="AAR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033648">
        <property type="entry name" value="AAR2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038514">
        <property type="entry name" value="AAR2_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033647">
        <property type="entry name" value="Aar2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038516">
        <property type="entry name" value="AAR2_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12689">
        <property type="entry name" value="A1 CISTRON SPLICING FACTOR AAR2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12689:SF4">
        <property type="entry name" value="PROTEIN AAR2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05282">
        <property type="entry name" value="AAR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20981">
        <property type="entry name" value="AAR2_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAR2 N-terminal" evidence="3">
        <location>
            <begin position="6"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="AAR2 C-terminal" evidence="2">
        <location>
            <begin position="181"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="40966" checksum="2F31CEB14386BF3C" modified="2017-01-18" version="1">MNTPPRTTIIFSNIPQDWLVGIDLQFFNTNHILKGIKLVPDGIHIVHFAKDQSSIRHGFYINAKEGDVIICYWDEKDEKILVDDEIGELNITKEMSKLSDYYSFMISYPPDPEWKKLTQYINFGQVNYILPRGKRIDSVLTSSDENNLLLDALQKSAKSRNLTQDPIINSIIDQSDEEIKYTLIDLNKTIRPDGTAGEKTKDSLDKSWFLNQLLISGYNSNEEMLLSEFQQAFLNMIIFANYSSSIQWLKFIKVIMNCKEILNGRLIFFEKFLDVLIVQFGKFQEDYMDEFISEDFLDTTISEFEYTVKEQELRPLIKKTIELKNLLLVKFSITIQGLMEDEEEAPVVVDISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PDL0</accession>
    <name>A0A1E3PDL0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_67221</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042249.1">
        <property type="nucleotide sequence ID" value="XM_019184075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PDL0"/>
    <dbReference type="GeneID" id="30201321"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ63042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="84" mass="9451" checksum="C89773AA54432A9B" modified="2017-01-18" version="1">MLVELVFPVVADESVEILDVILPVVEYFGGKEPTQVIFDHVESETTIFGHVIAEFTHAFGNKVEDFRPDRKTLGPMDFAIVDGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUB7</accession>
    <name>A0A1E3NUB7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">GPI ethanolamine phosphate transferase 1</fullName>
            <ecNumber evidence="13">2.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_81570</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Endoplasmic reticulum membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the PIGG/PIGN/PIGO family. PIGN subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.-.-.-" evidence="13"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035942.1">
        <property type="nucleotide sequence ID" value="XM_019185259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUB7"/>
    <dbReference type="STRING" id="683960.A0A1E3NUB7"/>
    <dbReference type="GeneID" id="30202505"/>
    <dbReference type="OrthoDB" id="2748310at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015867">
        <property type="term" value="P:ATP transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16020">
        <property type="entry name" value="GPI_EPT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000015">
        <property type="entry name" value="GPI ethanolamine phosphate transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007070">
        <property type="entry name" value="GPI_EtnP_transferase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017852">
        <property type="entry name" value="GPI_EtnP_transferase_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037671">
        <property type="entry name" value="PIGN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250:SF0">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04987">
        <property type="entry name" value="PigN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 13" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="459"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="496"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="520"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="557"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="581"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="606"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="630"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="704"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="729"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="790"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="831"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="862"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="889"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase N-terminal" evidence="14">
        <location>
            <begin position="217"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="GPI ethanolamine phosphate transferase 1 C-terminal" evidence="15">
        <location>
            <begin position="449"/>
            <end position="888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ56735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="941" mass="107487" checksum="F87EFD85E53079D4" modified="2017-01-18" version="1">MVSILGNNRLVLLMVGVAFHLFYLWSIFDIYFVSPLVHGMEPQLSTPTPPAKRLFLIVGDGLRADTTFDNITHPTTGKTGYLAPFLRDLVLNEGTYGISHTRMPTESRPGHVAMIAGFYEDVSAVTKGWKENPVDFDSVLNQTKHTYSFGSPDILPMFAQGASDPNRIGTWMYGHEFEDFTSSSIELDAFVFEHLYDLFENSTKDHELKQQINQDQTVFFLHLLGCDTAGHGYRPYSAEYYDNVIYIDEQVAKLTKEVKKFFGDDDTAFIFTADHGMSAFGSHGDGHPNNTRTPLIAWGAGINKPVYNEVPIFDNYTENWQLGQIKRNDVKQADIASLMSYLIGVNYPANSVGELPLAYIDNKEEAKLKALYQNALSIVEQYHVKESEMKESQFFFKPYPRFEIKSIANYQSEIELLIDEISKSKKFEVEKEIEAIALVEELMKTTLDGLYYLTTYNWLLLRSIVTLGFIGWITYSFIIFLRLFILEKDVPTSTSFLNFAVFSSIGLIFNYILFYQRSPFNYYMYLIFPIFFWSQITSKSLILQQGLQSFFSGISHFKRFLILISIIGVFEGIVYGFFERIIFTIFFIALSFYPVLLGVSDLKTLIFWGITNIGMSTYTLFDAVKVEDLYQINAAAVLISIFGFVCYFKILQNSHSEKNFPTISSYTKNLINVQISLIALMTLVTNKSVSSLQSREGLPKSSQFLGWLIFVLSLFVVPFLHYLKPHNDYRIRLLIIFLTFAPTFIILTISFEALFYINFSILLLQWIKLETDYFQRKGGKIENYSQLLRVSVIGFLYLQVCFFGTGNVSSISSFSLDSVYRLLPIFDPFPMGALLMLKLIIPYVLLSTALGVLNVKLGIKPYTISTLIISTSDILSLNFFYLLKTEGSWLDIGVTISNYCLAILSSLFMLILEIISHVLLKNVEYHDELEARGEEEVKKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUH5</accession>
    <name>A0A1E3NUH5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_37026</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035940.1">
        <property type="nucleotide sequence ID" value="XM_019182504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUH5"/>
    <dbReference type="GeneID" id="30199750"/>
    <dbReference type="OrthoDB" id="3026777at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507:SF1">
        <property type="entry name" value="FI18259P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507">
        <property type="entry name" value="ZGC:174356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="345"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="383"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="484"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="518"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ56733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="582" mass="64849" checksum="9560447F50E5C67D" modified="2017-01-18" version="1">MRKDTTTTETSTLLRDNDAIIENAAVDVFESVITDQDDPDDNEDIRWLREERLHHQNKLWYEKPTVFLLSLALLLYFTSMGIQISAKIMLLMKQICRKVAEDHPDLSEEYINNNCRGPLVQGELSFLTSIVTFFTGAFSCLMSGKMGELSDRFGRKPILAIVALTTLLGRCFTAYLLMDGRTYHRGLFILSSVVEEMTGGNTVVVSVSSSYITDIVEPHERIVSLGFMIGTLYGGLAIGPLLGNIVIKYIGHGNDIYALYAGLVLCALFALILVFVIKESRPMKLRRKSQSTHLRRKASFTSSIHSSQSTSMYNQFQLWRIVDIFSPLKKLWIAKHPEQGFKPRINVLIMIFSDCVLSLASAAYTSTLVLYTTYYFNWGTDNIGYYVSMVGFSKSFCLYAVSPVLLHFLKKFLTIKKKSIDSVDLAVIFIGLIFDSLGPLTVIFAIQSSQVYLSAIFASLGALAGPTIQSSIVKYVSETQTGEVFGAIALIKNFILCLGPPIFLQIYSKSVATFPTLVFWITVFMFIISIALSTFLRSHKESEDGSIVTTIVDADEEEAAMLINQSDNGLFKAPSVGNSSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUN0</accession>
    <name>A0A1E3NUN0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">D-arabinitol 2-dehydrogenase [ribulose-forming]</fullName>
            <ecNumber evidence="5">1.1.1.250</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_36768</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; D-arabinitol metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.250" evidence="5"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036045.1">
        <property type="nucleotide sequence ID" value="XM_019182472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUN0"/>
    <dbReference type="STRING" id="683960.A0A1E3NUN0"/>
    <dbReference type="GeneID" id="30199718"/>
    <dbReference type="OrthoDB" id="5325318at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047038">
        <property type="term" value="F:D-arabinitol 2-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000240">
        <property type="entry name" value="SDR family oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
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        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF14">
        <property type="entry name" value="DEHYDROGENASE ARBD, PUTATIVE-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GDHRDH"/>
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    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
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    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00066831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00070881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ56838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="278" mass="29908" checksum="F5864BCA8B6DA69E" modified="2017-01-18" version="1">MTLEVAETIADIVPSFRLDGRIAVVTGGAGGLAITLTRALLSQGAHIALVDLNQQVCDAAAQDLALWGQNQGLNPLPNISSWSCNIGNADEVELTVSKINEYYNDVADLLIHTAGYCQNYTALEYPNQKAADLVQVNLVGSYYIAKAVAKHLVDAQKPGSLVLIGSMSGVIVNDPQPQVAYNMAKAGVIHLTKSLATEWAKYGIRVNCLSPGYILTPLTKHVIGNDTTLKNAWESKIPMHRMADPKEFVGSVLYFASHDASSYTTGENLIVDGGYQCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV04</accession>
    <name>A0A1E3NV04_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">biotin synthase</fullName>
            <ecNumber evidence="3">2.8.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_102389</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="13">Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="13">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the radical SAM superfamily. Biotin synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.6" evidence="3"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036077.1">
        <property type="nucleotide sequence ID" value="XM_019180349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV04"/>
    <dbReference type="STRING" id="683960.A0A1E3NV04"/>
    <dbReference type="GeneID" id="30197595"/>
    <dbReference type="OrthoDB" id="2414104at2759"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00162"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000011">
        <property type="entry name" value="Biotin synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01694">
        <property type="entry name" value="BioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024177">
        <property type="entry name" value="Biotin_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00433">
        <property type="entry name" value="bioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="BIOTIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976:SF2">
        <property type="entry name" value="BIOTIN SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001619">
        <property type="entry name" value="Biotin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01060">
        <property type="entry name" value="BATS_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00272">
        <property type="entry name" value="biotin_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01278">
        <property type="entry name" value="biotin_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4 13" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11 13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="14">
        <location>
            <begin position="99"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001619-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ56870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="388" mass="43660" checksum="0992E84F415650EA" modified="2017-01-18" version="1">MNRSRTLTAYTFRQLRSFHNSIALARPPAVSATAPEPEPEFFEEQIQVQENVGGQSALELAMTTNKPLNTWTKSQIKEIYQTPLIELIHSAQLQHRKFHDPTKVQLCTLMNIKSGGCTEDCKYCAQSTRYNTGVKAERMVQVDEVIKAAEEAKANGSTRFCMGAAWRDMTGRKNGLKKVGEMVKAVNDMGMESCVTLGMVDKEQAKQLKDAGLTAYNHNIDTSREHYKKVISTRSYDDRLKTIKNVQDSGIKACTGGILGLGETEDDHIGFLYTLSTMTAHPESLPINRLVPIKGTPMYEEVKHKQVKFDEIVRTVATARLIMPEAIIRLAAGRYTMKEHEQAMCFMSGCNAIFTGKKMLTTMCNGWDEDKAIIWKTENRLKKFSLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV69</accession>
    <name>A0A1E3NV69_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA topoisomerase (ATP-hydrolyzing)</fullName>
            <ecNumber evidence="4">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_36588</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the TOP6A family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="4"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036202.1">
        <property type="nucleotide sequence ID" value="XM_019182451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV69"/>
    <dbReference type="STRING" id="683960.A0A1E3NV69"/>
    <dbReference type="GeneID" id="30199697"/>
    <dbReference type="OrthoDB" id="5377392at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000706">
        <property type="term" value="P:meiotic DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00223">
        <property type="entry name" value="TOPRIM_TopoIIB_SPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002815">
        <property type="entry name" value="Spo11/TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013049">
        <property type="entry name" value="Spo11/TopoVI_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036078">
        <property type="entry name" value="Spo11/TopoVI_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034136">
        <property type="entry name" value="TOPRIM_Topo6A/Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848:SF0">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21180">
        <property type="entry name" value="TOP6A-Spo11_Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04406">
        <property type="entry name" value="TP6A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01550">
        <property type="entry name" value="TOP6AFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56726">
        <property type="entry name" value="DNA topoisomerase IV, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52041">
        <property type="entry name" value="TOPO_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Spo11/DNA topoisomerase VI subunit A N-terminal" evidence="11">
        <location>
            <begin position="2"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Topoisomerase 6 subunit A/Spo11 TOPRIM" evidence="12">
        <location>
            <begin position="110"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="10">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ56995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="287" mass="32634" checksum="F5A6C03DC2072975" modified="2017-01-18" version="1" fragment="single">AMYTRLISLLIENLEKNIITTKRDLYYQDVSLFQNQANVDAIIEDLCNVLGFTNQELGAVAALKGLCFGDVTLLNGNPNLDQIFTRQQGSFLIPRLNNKSKIQLSNDVQYILVVEKDAVFNLLCDPTNSNRIIVTGKGFPDHLTKRFLSLLSITYPHLPVLGIADIDPYGLNIMKQFKSGGDKSNCFMVNKDQKFDCPRLKLVDITILDFIKKLDCLDLTIRDFTLSSKMLELDFYNDVQQEIWRSELQRSMFFGKKAEIDVIKGSNDNLEINLTQYIANAIDKAMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV77</accession>
    <name>A0A1E3NV77_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_16371</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036255.1">
        <property type="nucleotide sequence ID" value="XM_019180688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV77"/>
    <dbReference type="STRING" id="683960.A0A1E3NV77"/>
    <dbReference type="GeneID" id="30197934"/>
    <dbReference type="OrthoDB" id="343875at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000791">
        <property type="term" value="C:euchromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990269">
        <property type="term" value="F:RNA polymerase II C-terminal domain phosphoserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045142">
        <property type="term" value="F:triplex DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901525">
        <property type="term" value="P:negative regulation of mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001209">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060260">
        <property type="term" value="P:regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090262">
        <property type="term" value="P:regulation of transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031126">
        <property type="term" value="P:sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001015">
        <property type="term" value="P:snoRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031101">
        <property type="entry name" value="Ctr9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14027">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN CTR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14027:SF2">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN CTR9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="192"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="309"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="686"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="865"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="913"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="954"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="999"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1012"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1021"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1021" mass="116810" checksum="1625DA37DD0694F5" modified="2017-01-18" version="1" fragment="single">IIEVPIKDSREVVTIDLEHELPEDPTDLCTLLENESAESIYWISIASVYANKGLVSEAFEMMTEGLKLYKELDTADFHTFLAWLHIRQSKVAKTSDDQRASLKMASESIKLALAAQPTNVLNRIAQAEISYQLKQYDQALDFFERILKNDSKSTANVFALTGKAKTLFHKKNYNASLKIFQQVLILNPLLKPDPRIGIGMNFWRLNDKNMATKSWERALEIDPENQTAKLLLTLSKFDSSFNSLTDEDFISNYSAALNSLNEFVQKDSNNPVTLLILASYYYSKNDLESVLKVSQKVLSSPNASPDLLSDANFWIARTHYENGDFSNAQKFFTESLKSNPDSLLAKLGVGQSQFNRSLLDDAILTFESVYKNNQKILEINYILGILYSQDEKNHEKAVPVLERYIRLSKDKNEPVALNAYLTLSRLHENKDNSQSLSLLSKVIDILKKRGEDVPVEILNNLGVFHFIKGNPESANTFFKSAFEANQSEDLNITLNYNIARTEENSNTEDSISKYNDILEKSPGYIYSKIRLLFLQIISSDVKNDEELEKKLNDLLQSHVSNLEVRAFYSWYLKRTNRSSNDKGENLESKHNKETLVKYDSHDTYALISLGNLYCAIAREIKGKTPQDIEKKNQSYVRGAQLFQKVLSIDPNNVFAAQGIAIIFVENKHSNIALETFRKIRDAIDDVSVYINLGHCLIEVGQYSKAIESYEIALRRYGDDTKDSRYLTLVGRAWFARASAEKSLESFLKSLEYSIKALDVAQNTNLKKLIPSLKYNVAFVQFNIAQFIQKQNIAKRTVDDINKALEGLKEAIDTLNDLANDKYPPYPAEILKQRASMGTNTLINQLERAIKEQKEYEIKFEHRLKEAQESREKERQKAELEEQRRKEEEAKKEAQYREEYLKLQEQAKEWEREREAFVEPSFDEQDGKKPRKGKKTKNAINTDDELSGDDEKPVKKTKKSTGKRSKRKKDENATNGNVEGGDPEPKKRKLTSKKYLSADIIEDSDEDLEDFDESKLGGDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV78</accession>
    <name>A0A1E3NV78_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_58527</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the gamma-BBH/TMLD family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036201.1">
        <property type="nucleotide sequence ID" value="XM_019182825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV78"/>
    <dbReference type="STRING" id="683960.A0A1E3NV78"/>
    <dbReference type="GeneID" id="30200071"/>
    <dbReference type="OrthoDB" id="406634at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045329">
        <property type="term" value="P:carnitine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00250">
        <property type="entry name" value="CAS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2020.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050411">
        <property type="entry name" value="AlphaKG_dependent_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010376">
        <property type="entry name" value="GBBH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038492">
        <property type="entry name" value="GBBH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696">
        <property type="entry name" value="GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696:SF25">
        <property type="entry name" value="OXIDOREDUCTASE AIM17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06155">
        <property type="entry name" value="GBBH-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma-butyrobetaine hydroxylase-like N-terminal" evidence="8">
        <location>
            <begin position="57"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="160"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ56994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="437" mass="50438" checksum="AE0E3B0FF19CCB78" modified="2017-01-18" version="1">MLRSKIKPASTFNSFRSFIKQRSISTEPSTPAINEVVGNNIKVIHNHKWTSFEFLNQNKSFSYNNTFLRDSSTSSSSIDPESGQKLFTTASIIESYPKSIEFPANDRIQITWNDGEISEFDQSFLESNATIENRKFGKYFDEDIVTWDINKELTIDNVPKIDYNDYLTKKESISKVLENLNKYGLSQIINIPTKSDKDHDSSPIVQQIGERIGYIKQTFYGTVFDVRSVPNAKNIAYTSQFLPLHQDLLYYESPPGLQLLHFIKNKSIGGENVFADGFAAARHVKTVDPEAYLALQSVPINYHYDKDGHSYYYSRPLIVENEVVGQGVYGEKDHFKEVNYSPPFQAPFDFGITELGDRHVFQDFLRGLKEFEDYINDPENQLKFKVEENTCIIFDNRRVLHARDQFDNNSGERWLKGCYIDKDAYLSKLRVTKKQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVN2</accession>
    <name>A0A1E3NVN2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal RNA-processing protein 40</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_35899</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRP40 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036380.1">
        <property type="nucleotide sequence ID" value="XM_019182370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVN2"/>
    <dbReference type="STRING" id="683960.A0A1E3NVN2"/>
    <dbReference type="GeneID" id="30199616"/>
    <dbReference type="OrthoDB" id="340500at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071034">
        <property type="term" value="P:CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22526">
        <property type="entry name" value="KH-I_Rrp40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05790">
        <property type="entry name" value="S1_Rrp40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000127">
        <property type="entry name" value="Exosome complex component RRP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026699">
        <property type="entry name" value="Exosome_RNA_bind1/RRP40/RRP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049469">
        <property type="entry name" value="RRP40_KH-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041054">
        <property type="entry name" value="Rrp40_N_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037319">
        <property type="entry name" value="Rrp40_S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321:SF1">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321">
        <property type="entry name" value="PNAS-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15985">
        <property type="entry name" value="KH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18311">
        <property type="entry name" value="Rrp40_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21262">
        <property type="entry name" value="RRP40_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exosome complex exonuclease Rrp40 N-terminal" evidence="11">
        <location>
            <begin position="19"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="10">
        <location>
            <begin position="147"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF15985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ57173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="229" mass="24928" checksum="CC0EF7824638D8EC" modified="2017-01-18" version="1">MSQLIIPGDNLTISDSKELVIGPGIHVTPITKEIIPVHSGILQQANNALHIESNSRRYIPSNNDYVIGLVTGVFGDNFRVSLSNFSFPVSLSAMAFPNATKKNRPNLKIGNLIYARVSNASPEVDVEIECIDPTTGKDGGFGLLEGGYVFDVSLSFARSLLFDVNHTLLNLLVKKCKFELAIGLNGKIWIKADDLKYTLALAKSIEKAQLVYSGEFNKVINSAFSELGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVP1</accession>
    <name>A0A1E3NVP1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phenylalanine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_81404</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="3"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036390.1">
        <property type="nucleotide sequence ID" value="XM_019185229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVP1"/>
    <dbReference type="STRING" id="683960.A0A1E3NVP1"/>
    <dbReference type="GeneID" id="30202475"/>
    <dbReference type="OrthoDB" id="238316at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000028">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040725">
        <property type="entry name" value="PheRS_DBD3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003210">
        <property type="entry name" value="PRK04172.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF40">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18553">
        <property type="entry name" value="PheRS_DBD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="226"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ57183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="496" mass="56382" checksum="90A2E96539FF4158" modified="2017-01-18" version="1">MSELQLTILQTLDKEGSIENTLNTFPKLDSQEVQAALNSLLARSKITYKSIEKEKWNLTKEAEEIVQNGSHEYRLLETILKLEHLKISEVGNVLGPAGKTGQARAFKNGWISKNGDELVVSDKLDKSQLPSDETSTELKEVQETGTLKDAKKLSELKKRKLVTVSKILSFKVEKGPEFSLEIEKLETDITSDMVISGNWENAKFKPYNFNSEGVYPDSGALHPLMKVREEFRQIFFSLGFSEMPSNQYVETGFWNFDTLFVPQQHPARDLQDTFYIKDPLVAGAPEDKTYMKNIKDVHEGNAFGSIGYRYPWNEEESLKLVLRTHTTAISSRILHELAKDPKPSRLFSIDRVFRNEAVDATHLAEFHQVEGVLADYDLTLGDLIGFMEDFFGKMGVKDLRFKPTYNPYTEPSMEIFSWHSGLGKWVEIGNSGMFRPEMLEPMGLPKNLRVLGWGLSLERPTMIKYGVSNIRELLGHKVSLDFIETNPAARLDEDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW08</accession>
    <name>A0A1E3NW08_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_81401</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036387.1">
        <property type="nucleotide sequence ID" value="XM_019185227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW08"/>
    <dbReference type="GeneID" id="30202473"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="524" mass="60609" checksum="E34AA2BF0F47220B" modified="2017-01-18" version="1">MRSPYDIVVNDVILRLWRLVVWLIAETLWQLVVWLISTTLVTPLLYLLIFPIYYQWQKSARAQRLSQYLTHILLSKHLIVPIERCCYHSLYGMKTGLLKLKCPTEHEFNTFFDLPDELMTQIAFITDVNISHLLQLNKKCVFAFGPILYQKVHFSIPVISTEVLTTAGHIRLRSPQNTTGAKLRVNAYLRNTTQFSSTTNQLGILGQKYQLLQRNKKADNHDFMGFFSSSKRKPYKRTLKDRMTHVWDHDLMIEICNNVMSNEKSIMRQFIRQFFVDIRIFSGVYDPSYSISRNPGITLIKTRKQGEIKSTHSATQSATYTDIIFKKDLSNLESANSSANEHGSYKALIEPCLNLCKNKELLVSSFYDMLIFQGQETLVADVKHSNIDPPHLVLLTTDSFEILRVLYNIGYSFLGDENDLNLYDEQGYFSSTFGEHSIDIKNLNSLDEPDFYMGSEVCDLIEMVSNAALSIDETPARRSSYMGFSHETLCLYETWYEYNRTVASREPFTIEHQIKIFVIKDDGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWF9</accession>
    <name>A0A1E3NWF9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Alkaline ceramidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_64803</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alkaline ceramidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036662.1">
        <property type="nucleotide sequence ID" value="XM_019183808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWF9"/>
    <dbReference type="STRING" id="683960.A0A1E3NWF9"/>
    <dbReference type="GeneID" id="30201054"/>
    <dbReference type="OrthoDB" id="187171at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046514">
        <property type="term" value="P:ceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008901">
        <property type="entry name" value="ACER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46187">
        <property type="entry name" value="ALKALINE CERAMIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46187:SF3">
        <property type="entry name" value="ALKALINE CERAMIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05875">
        <property type="entry name" value="Ceramidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR608901-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR608901-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="285" mass="33099" checksum="606419D641C02382" modified="2017-01-18" version="1">MYQLPFAQPYPAVPDGGYWDKVTSTIDWCEENYVVTPYIAEAMNTLSNSVFIILAGYAMYSAFKNHLEVRFILISFGFALVGVGSWLFHMTLKYEFQLLDELPMIYATCIPMWSVFSEGVTTKKSITIGTSVLFGANLLTAVYLYFKDPTIHQVAYALLNVFIVGRSHYLTLKFVKDPVDRAKLFRTMIRGVVTFLTGYALWNMDVHFCNSWIKLRRNIGMPYGFFFELHSWWHVLTGTGVYFYVVYLEYLRVLLLKQEDHYVLVEHLGFLPEVQRLEKPISKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWG3</accession>
    <name>A0A1E3NWG3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoglycerate mutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_57554</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036547.1">
        <property type="nucleotide sequence ID" value="XM_019182806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWG3"/>
    <dbReference type="GeneID" id="30200052"/>
    <dbReference type="OrthoDB" id="496981at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF1">
        <property type="entry name" value="HISTIDINE PHOSPHATASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="32012" checksum="ABEC531F2429A375" modified="2017-01-18" version="1">MYIPKLLKYSLTFAAQDFQALDYFTQYSNSSSVTTDLPNFGLKSEHSWSSFLQDIQDLNDKSDDTRYKLIFLARHGEAWHNVLGSLPNWYEISRQDTYKNYTLFDDDLTPTGESQISKVHDYWINELNNGAPYPQVFYTSPLRRTLHTYHITWGVENGSAIVVENLRERYGIETPEERHNKTWISDYCNVCEFEKGFAQDDELWKPDEQESKKHVRKRAKEWIQSLFIRDEHIISVTSHSGTIKQILKVIGHPKHSLQPGELIPVVVKGRKSHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX31</accession>
    <name>A0A1E3NX31_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_97115</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036913.1">
        <property type="nucleotide sequence ID" value="XM_019186561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX31"/>
    <dbReference type="STRING" id="683960.A0A1E3NX31"/>
    <dbReference type="GeneID" id="30203807"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05227">
        <property type="entry name" value="AR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="349" mass="38874" checksum="3E9744CD091A7A82" modified="2017-01-18" version="1">MSTKTVLLTGASGFIALHILDLLLKKGHHVIGTIRSPEKGEKLKAQFQKEHPNSQLEFEIVHDIATEGAFNEVLKNHPEITDVLHTASPFSFGLNKDLKEAYWVPATQGTKNVLEAIKAYAPQVKHVVITSSFAAIVNREKGGDETFIHTEETWNPITWDDVDGEAKAYTASKKLAEELARKFVRDENPNFTLTTVNPPYVFGPQKFEDSLVSPNLNTSAEIVNKLLSSSPNDTHFFEQPFGTSVDVRDVALLHVLPIENDELAGKRLFPTQAAFNGQVLLNIINKEFPELNGKIAKGSPEGAEEKVKETETFYDTSKTLKWAGIKQWIPLEQTLKDAVSQILTFRKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX82</accession>
    <name>A0A1E3NX82_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Brl1/Brr6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_19756</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036912.1">
        <property type="nucleotide sequence ID" value="XM_019180842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX82"/>
    <dbReference type="STRING" id="683960.A0A1E3NX82"/>
    <dbReference type="GeneID" id="30198088"/>
    <dbReference type="OrthoDB" id="5961at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040202">
        <property type="entry name" value="Brl1/Brr6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018767">
        <property type="entry name" value="Brl1/Brr6_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136:SF1">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10104">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01042">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Brl1/Brr6" evidence="2">
        <location>
            <begin position="4"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="139" mass="15682" checksum="38CE8009B4D60613" modified="2017-01-18" version="1" fragment="single">PYILSLYLQLIFNVLIVSILLYFVYSFISTVKADVENKVDSYASDIIQEIALCSREYLRNNCQPGKRVVALEAACSQWEKCMNQDPTTVGRAKIGAETFAEIINGFIKPISWKSMTFLFLITVGSLVLTNAAFGTYRNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXE8</accession>
    <name>A0A1E3NXE8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_80038</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037078.1">
        <property type="nucleotide sequence ID" value="XM_019184991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXE8"/>
    <dbReference type="STRING" id="683960.A0A1E3NXE8"/>
    <dbReference type="GeneID" id="30202237"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033229">
        <property type="term" value="F:cysteine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF63">
        <property type="entry name" value="HIGH AFFINITY CYSTEINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="396"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="429"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="462"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="59782" checksum="8D1D7CBCCB920A04" modified="2017-01-18" version="1">MSTSSNHSQLHDLEEKKSPQTNVISKQEVEEKGYRVIKSKDADATLKFQEQYDSTVPEITPEQEAKLSRKVTWIIMSLVCLVNLLLYMDKATLSYASIFEFWDDTGMDQNKYNNVNTLFYVGYIAGQIPGNYLLQKLPIGRFLFILTSLWTVIIFLHCAAYNYSGVIALRFFLGFVESVVLPLLNITMGQFLTASEKASTAPIFYSTCLGVTIPTGFIAYGVLFAKSSIHAWRIFMIIIGGLTFLLTVLVFWIYPNNPSTARFLSTQEKIWVIRRVQNTTGAAIEQKVFKKYQFIEAIKDPLTWLFGLFFFLQQLANNLPYQQNLLFTGMGGISNLNSTLVSVASGGFAVVCCLIASFILARKQNITAFSVVFWSLPSLVGSIVAVALPWSNKTGLLAALCLASPVFGIPWILMFSWNSTSCSGYTKKITRNAIVMFWYSVSNIISPQLWQARDAPRYYGAWIVQIVLSFTAAPALALIIYYVLNKRNKERLSNLSEERKVGVVEDSDVDFVVNVASLDLTDLENKAFIYPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXP9</accession>
    <name>A0A1E3NXP9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_80051</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037091.1">
        <property type="nucleotide sequence ID" value="XM_019184994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXP9"/>
    <dbReference type="GeneID" id="30202240"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="388" mass="45026" checksum="F9295EE72FF3724C" modified="2017-01-18" version="1">MELLKLPRDIWDIIVFKHNLSAHDFMNLADASALFQSLVKEYVCVVSNDINHPSNALTANLIGFNMSGFNVSRLPIRFDFDDCMLDSTISSSMIIVFNFVLNKDFDNDLEFYLKSFGMKCNQHEKYYQFNFFSLQEKSTTNKDFKVLDTIKEWINCKHCTVFLEKYFLGYQVYELDRSIFEVGNNVVSDDNDNSQIVKHKKPKQDYIWQSIQKLFIQRQTIVLDNHSCSFDLINLRLQNLTSLTLSSSKFHSDSSKSNLDSSEYIPPFCLKDCEFPNLISFTTLNGARIQSIINCKFNKLQLFDVHCSFWFIMRRVQLKTVQDFKLSSSSRYSSNYPKPKNIDPPNELAGCELQLSKKYSISSNLVKKIMCYLEVSVHDISLTLSSQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXS1</accession>
    <name>A0A1E3NXS1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_70481</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036564.1">
        <property type="nucleotide sequence ID" value="XM_019184208.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3NXS1"/>
    <dbReference type="GeneID" id="30201454"/>
    <dbReference type="OrthoDB" id="6109at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="6">
        <location>
            <begin position="12"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="7">
        <location>
            <begin position="494"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="5">
        <location>
            <begin position="932"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="384"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="400"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1293" mass="147089" checksum="B0AEE3780314DD1F" modified="2017-01-18" version="1">MDVYDQFIDPTSVSHSVACNFTSLESKDLIVVKNTLLQIFKVKNGKLVLINEFQLNGKVIEIKPALLPNSKYDQLLILTKFAKLSIVSFDFEHNSIQTNSLHYYESEFSSKTISQLDESSLRVDPNNNSSLVFYNDILAFLPFKQDEEEIEIDDTRILDKDREIFDKSIIVPVHKLESTITKIMDCDFLFNYRDPTLGILYRTDLTWASDLSKKKDVVNFVVLSLDLANDSSTPILQVENLPYDLWDVKPMPDPINGALLIGCNEIIHVDNSGNTKGIGVNLYYPDCTDFKLIDQSDLNIFLENSKIEVITRNDVLIIDESGNFYNLHFTIDGKVVKDLNITRISTKLPIIRPITVSKIDDDHLFLGCVSSDSVVLTVNKTQQVPQNGDGKIKEATTDNTNDDDDDDDDDLYNDEDDAGNGTNQNDVFNFDFSIQDFLFNAGPISSFTLGQINSNPFVQGLENPNKNDVSIVTTSGEGEQGALTVFKPTIQPIIHSTLKFSNISKTWNILNKYLVTTDLSNFKSEIFLINENFKNFQSFDFKNNNITINIDTIQSKKRILQITSTNVYLFDLNFKKLLQINFDFEIINGKIFDPFIILTSAKGEVKIFEMDPKGRKLNKVKVPKLISDLIITFATISSTKIVNGFNKRGAKRSLDNNPIENKNIVFLVTTVNNQILVFEKNHNEQVYQIDQINKLTEIANFKPYEEVKGLIPDPFIKEIEFTEIGDEVHKEEILTILTIGGELILYKIFKNSNNHYDFQKIENLNAITGAPENIWAESTIIERKLIKLKINNKDVIFVTGKQPYLIYKDPKSQPKLFKFTSKSAISFCNIHDGSNKNFEGGEHGDDLKFMYIDADKNARICSLPFGENFNYSQNLSIERFPLGQTANKITYHETSNLFIVSTFEEVPYNAVDEEGNDIVGSEKDKPSAKNFKSYLKLINPINWVVIDEVELDENEVVNDVRSIYLTISSRSKKKKEFIIYGVGKYRLEDLSVFGSFKIIDVISIVPDPTKPEANFKFKEIFQEVVKGAVTTITEISGRFLAGQGQKVIIRDLQQDNSTVPVAFLDCATYISDSKSFGNLLLISDSMKSVWFLGFDAEPYRLILLGKDLQRLNAICTDFIVDNGEVYFLVADDEFNLHLLTYQPDDPKSLSGQRLLKRTTFTTNTLTTALKMIPKYEEFDQGTASSSFQNIGVNVDGSIFKMLPINESTYRRLYILQQQLSDKVAHYVGLNPRSNRFGGNELGLKPIIDYGLIKWFINFNVDKRKQFSAKVGKNAYLELWRDFIEIENSIRNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY52</accession>
    <name>A0A1E3NY52_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">L2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">RP2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">YL2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_85172</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). uL4 is associated with the polypeptide exit tunnel. uL4 interacts with its chaperone ACL4 and the nuclear import receptor KAP104.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037232.1">
        <property type="nucleotide sequence ID" value="XM_019185854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY52"/>
    <dbReference type="STRING" id="683960.A0A1E3NY52"/>
    <dbReference type="GeneID" id="30203100"/>
    <dbReference type="OrthoDB" id="10259785at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1370.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025755">
        <property type="entry name" value="Ribos_uL4_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013000">
        <property type="entry name" value="Ribosomal_uL4_euk/arc_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_uL4_euk/arch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14374">
        <property type="entry name" value="Ribos_L4_asso_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00939">
        <property type="entry name" value="RIBOSOMAL_L1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL4 C-terminal" evidence="9">
        <location>
            <begin position="273"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00064557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00077544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00079326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="364" mass="39195" checksum="7675793D89AB69E6" modified="2017-01-18" version="1">MSARPTVSVYSTSGEVSSTSLPLPSVFSAPIRPDIVHSVFTAVNKNKRQAYAVAENAGHQTSAESWGTGRAVARIPRVGGGGTHRSGQAAFGNMCRGGRMFAPTKTWRKWHVKVNHNEKRYATASAIAASGVPSLVLARGHRVEEIPEVPLVVSSEVESITKTKEAVAVLKAVGAHRDVVKVLKSKKLRAGKGKLRGRRYTQRRGPLVVYGEDKGLVKAFRNIPGVETASVSALGLLQLAPGAHLGRFVIWTESAFAKLDSVWGSESVASAKSGYSLPNNIISNADVTRIINSSEIQNVVRPAGQATQKRTHVLKKNPLKNKQVLLRLNPYAKVFSAEKLGSKKVEQKSAKPSKGQFAEVLKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ94</accession>
    <name>A0A1E3NZ94_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_32172</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DAMOX/DASOX family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037768.1">
        <property type="nucleotide sequence ID" value="XM_019181925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ94"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ94"/>
    <dbReference type="GeneID" id="30199171"/>
    <dbReference type="OrthoDB" id="2015447at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003884">
        <property type="term" value="F:D-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006181">
        <property type="entry name" value="D-amino_acid_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023209">
        <property type="entry name" value="DAO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530">
        <property type="entry name" value="D-AMINO ACID OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530:SF11">
        <property type="entry name" value="D-ASPARTATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000189">
        <property type="entry name" value="D-aa_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00677">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="7">
        <location>
            <begin position="4"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="47"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000189-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="341" mass="38074" checksum="B310E7BECB8C95F8" modified="2017-01-18" version="1">MAKYVVLGAGIIGLYTTYHLIHTLGIDGKDIKIIAEYFPGDQSINYTSPWAGGNFSCITSDDEDVLRFDELTYTNLQSILEKIGKSAGLARKKSTELFDTYPGDLKVASLKSYLEEFKPITKDNLPKGVEYGFTFLGWNFNCPIFLSNFLIYLKSLGVQVSRTKVEHISQAFLPKSKVVFNCTGIGARSLPGVQDSKVYPTRGQVVVVQAPHIQESRMRWGEDYATYIIPRPDSNDELVLGGFLQKENWTGDTFLSETEDIIKRTTELCPEILDQPLKIMRVAAGLRPSRHGGVRIEVENIEGKYLVHNYGASGYGYQAGFGMAYKAIELYAKESKIKSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZG0</accession>
    <name>A0A1E3NZG0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_85553</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037760.1">
        <property type="nucleotide sequence ID" value="XM_019185909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZG0"/>
    <dbReference type="STRING" id="683960.A0A1E3NZG0"/>
    <dbReference type="GeneID" id="30203155"/>
    <dbReference type="OrthoDB" id="5290825at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015798">
        <property type="term" value="P:myo-inositol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015791">
        <property type="term" value="P:polyol transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="407"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="442"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="474"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="505"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="93"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="556" mass="61351" checksum="3A08E6619C7C785D" modified="2017-01-18" version="1">MSVEKDQIENHISEDEKTVFKPHQLEESVSKEVEEIIQNYDLEVYRELLSRGALLANNPDLLFTDKYTESEQNAISKESTHPLLSLSPALILAALTTSFAAVNFGMDESAVGGAQLQYNKQFGITDVNLQGLVIASPYLAAAVIGCPLTVFLNNHLGRKWVIFISCFIGFTGSLWQGFSNGLASLLVGRLYLGIGMGLNSTTVPIYAAECSPAVSRGAILMLWQTFIALGVCLGSVFNRAFVEIDGSLSWRLMIGSSCVPPLICGIFVLFPPESPRWLIANKKTRQSFEALLKLRSSRVSGAKDFYILYESLKFENKLSQNLSLSRQFLDLFLVRRNRFAVWVSFLGIFGQQYGGVNILVSYTTTILTNAGIDSITAIAGSIGIGGGCFLATFISTQLIDRYGRKKMLLFTLPVEGICLFWLGGVLNVTNDKARLGAGLTSMYVYVLFYGLGIGPVSFALVAETPSITVRSAHSAFLMATNWLLDFCLSMTWPKMNSTMTTSGGLYFYGAWNIILVILVFFYVPETKRYTLEELDEVFEEGAKSFSKKQIKKILGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZI9</accession>
    <name>A0A1E3NZI9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mtf2-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_64246</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037309.1">
        <property type="nucleotide sequence ID" value="XM_019183694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZI9"/>
    <dbReference type="STRING" id="683960.A0A1E3NZI9"/>
    <dbReference type="GeneID" id="30200940"/>
    <dbReference type="OrthoDB" id="4096061at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043837">
        <property type="entry name" value="Mtf2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040009">
        <property type="entry name" value="Mtf2/C5D6.12-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39468">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39468:SF1">
        <property type="entry name" value="MTF2-LIKE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19189">
        <property type="entry name" value="Mtf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mtf2-like C-terminal" evidence="1">
        <location>
            <begin position="132"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="347" mass="39558" checksum="A4B002133265D58E" modified="2017-01-18" version="1">MLRSSIKCCYRRSVTRSFSLARPNKNQGSSQVPDDILANFAKNTASKQDSDTFTSIFNNIMTFKKDTSHSSKNDLPSLQVIFEKNLYSGGAKTLSVNESKDLRSLPLSLSGDIVAQEFSPSGALIQQIQTKHELKEAISPTLQHLNKIDTDYFLIKYIESLIKEFSKYINSKNYKYEPISTELITKIKERSETDPNSPLINEFTIPIFLSTSINLLCLRFNSPLEAISIFQHIKNNLDIKTYTFSCTTEVYNEILERSWQFFRDPYMIDSLINEMKVNGISGDMDTISILGLVTNDMEKTLLGTTATELEDERQLGTTGYYGQFWNSEDEKLMKNVKAFRTDLMTSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZL7</accession>
    <name>A0A1E3NZL7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA-processing protein 42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_32114</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037863.1">
        <property type="nucleotide sequence ID" value="XM_019181919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZL7"/>
    <dbReference type="STRING" id="683960.A0A1E3NZL7"/>
    <dbReference type="GeneID" id="30199165"/>
    <dbReference type="OrthoDB" id="272245at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0271">Exosome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="7">
        <location>
            <begin position="30"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="264" mass="29316" checksum="E68D27B184C04F59" modified="2017-01-18" version="1">MILSATERSYLYESLQQSPPIRPDGRSIKQFRPIQSHIGFLDNSNGSAKITLSDGSECIVSIKAQVVDKSTTPDLIDVDVDVAGYRDDSSFILNLIATLKTLYSKHISKESLNLTTKFAYKLFIDVSVISNYSHPLTLISFTTYLALNNTYLPKITSSVDDKEIEEQPTFHDYEFNKLTGKFPIIFTLAIVGKNYILDPNVEEMEVSDNGLIVSWFDGKPISPTENVKLNDDNLKSVKPQLILSSINLVKEIAGSVIASLDAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZM7</accession>
    <name>A0A1E3NZM7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_64663</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037735.1">
        <property type="nucleotide sequence ID" value="XM_019183781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZM7"/>
    <dbReference type="STRING" id="683960.A0A1E3NZM7"/>
    <dbReference type="GeneID" id="30201027"/>
    <dbReference type="OrthoDB" id="506431at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052061">
        <property type="entry name" value="PTE-AB_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260">
        <property type="entry name" value="UPF0644 PROTEIN PB2B4.06"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260:SF1">
        <property type="entry name" value="UPF0644 PROTEIN PB2B4.06"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="31281" checksum="7553378D63A9EF11" modified="2017-01-18" version="1">MFLRSVKSLKISGGCSGALFRRIGSKSVRFNSTNASINPNVITPGESGKGNDNNNKNDGKKYSSNIPFYITALILGVATAQYLPISSIAKFLISEKLPKPNTPEAEEFKASLEKELEKLPIYKELCADPNYEPVRAWNYVDSNTLNQLITSGTLSTPGGFAIKPILFFNKSKKESVSIYHVGEKLCGYPFLVHGGILATILDEILKRTSTFSFGLDPLENYFPDKIKTKNIELQYKFPTVANSFLVVRCKDDGEKVDGRIETLDGKLLVKGSGTFEKRTGLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZR7</accession>
    <name>A0A1E3NZR7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_100685</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037860.1">
        <property type="nucleotide sequence ID" value="XM_019180302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZR7"/>
    <dbReference type="STRING" id="683960.A0A1E3NZR7"/>
    <dbReference type="GeneID" id="30197548"/>
    <dbReference type="OrthoDB" id="407410at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070939">
        <property type="term" value="C:Dsl1/NZR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060628">
        <property type="term" value="P:regulation of ER to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1420">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.670">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042044">
        <property type="entry name" value="EXOC6PINT-1/Sec15/Tip20_C_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007528">
        <property type="entry name" value="RINT1_Tip20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042042">
        <property type="entry name" value="Tip20p_domB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13520:SF0">
        <property type="entry name" value="RAD50-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13520">
        <property type="entry name" value="RAD50-INTERACTING PROTEIN 1 RINT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04437">
        <property type="entry name" value="RINT1_TIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51386">
        <property type="entry name" value="RINT1_TIP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="705" mass="82113" checksum="44D4BFF1124ED557" modified="2017-01-18" version="1">MSVLNQWFTGVQDLELVNDKIKELEAERLELQQLSDPNHHQTSSTIDFEELSKQLKFSIANSDIDTINALITGHGDIPSLVAAKNLVLERQRLLSLKSQQSQYKDLDEKLNEITYFDLIGLKELKNGISELTNLKYQATLYDKLDQLIISLKPKYQQALVESAGSSKWVNSSSIAPNLETDFHNLLNLQSLLNKQPKYPDTLWAFETLASNFKISFDYHFNTEKETNRIDRPELFLNYFTNYLSNHLSKFVGVFQLQDTEYSDRFAHSEFITALLKPVREKFSQVLKILRSSLEEENPKTQEANTQLLIHFIKETITFDQALVSDFYYDPLDDGNWEGLFALFNYTDLEKWLNYETKLNVANFESIINSSNCFHIDYTSVGDAQLKPTVSAIKLKYLFENITNSFSKFFLQNYENNSSLKKFKLKFFAKIYLRFLESYFQRLEDGFIAFNELFKKSRSVIHSAKNNTEIDITGTKGLERLFRIYCSLKYTINSLNYWNQEFIFIELNSLFNEYSTNKTLSLFSSILSDYNSLAEKTLQLINSFYSKTTTTLLRNYTQLNDWNDRSSPAPTTFTAQLASVIDTLQELNQFTVKVISTNEFLVVKNSLSYSVADYFFNNIIKSNHFSHKDFSIVWEKLQLSRKFPLYKKLIEAFQIMNLDDEQITQFGPFNTVNQFAKSGHFEILREVLQLEYLTDSDLLDLLLRIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZZ0</accession>
    <name>A0A1E3NZZ0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="12">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_33333</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="12"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037966.1">
        <property type="nucleotide sequence ID" value="XM_019182060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZZ0"/>
    <dbReference type="STRING" id="683960.A0A1E3NZZ0"/>
    <dbReference type="GeneID" id="30199306"/>
    <dbReference type="OrthoDB" id="10259640at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17941">
        <property type="entry name" value="DEADc_DDX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="41"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="72"/>
            <end position="246"/>
        </location>
    </feature>
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        <location>
            <begin position="273"/>
            <end position="432"/>
        </location>
    </feature>
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        <location>
            <begin position="592"/>
            <end position="733"/>
        </location>
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            <begin position="41"/>
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        </location>
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            <end position="664"/>
        </location>
    </feature>
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            <begin position="680"/>
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        </location>
    </feature>
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            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
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        </location>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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            <dbReference type="PROSITE" id="PS51192"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P006</accession>
    <name>A0A1E3P006_WICAA</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_84428</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019185710.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="30202956"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="76" mass="8729" checksum="70442B906D6C7726" modified="2017-01-18" version="1">MKYLEIVVYIDQVLIIYSSFTKINEVTTSITMSYHELNWISAYVAGKPIELSSVSEIKNQLSSNMENIPTELVERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0C7</accession>
    <name>A0A1E3P0C7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_31809</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
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    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ58805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019181884.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3P0C7"/>
    <dbReference type="GeneID" id="30199130"/>
    <dbReference type="OrthoDB" id="17687at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="GTPase activating protein (Gyp2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000015">
        <property type="entry name" value="GTPase activating protein (Gyp2)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
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    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="4">
        <location>
            <begin position="249"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="602"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="847"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="932"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="847"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="874"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="932"/>
            <end position="942"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="951" mass="108904" checksum="38FACFA51DEC93C4" modified="2017-01-18" version="1">MSFLNTLRERASSLSLFDKTNAPKKPSKDELFREEFKLPEGESILDETAAEMLVVSQADENQKGAARINDNQLTNHSNFVYSGKLYLTKHFLVFRDSFDRKSCVLVLNLSTIKKVERIPSKAYVFALSIVTNCGIKLLIQFIGIRSRSEEFSHKLKVNLRDNIPNTKKLEPFLNSLYSEFIVKKNTPGADKDSIKSPEGGLGLIFKFPGDPKKLRDKSKLRLWYDYFRRYGRNLSISRQKMFYKLLRVGLPNRLRGEIWELTTGAMYLRFQELSVYQKTLDDNAGKKSLAIEEIEKDLNRSLPEYSAYQSEEGIGRLRRVLTAYSWKNPDVGYCQAMNIVAAALLIFQTEEQAFWTLSVLCEKYVPGYYSKTMYGTLLDQKVFESLVEKTMPILWNHISKHDIQLSVVSLPWFLSLFLNSMPLVFAFRIIDIFFLHGPKTLFQVALAVLRINGEELLEIDDDGMFISVLKDYFSQLDDSAHPNSTDPKFRQLTKFQELLIVAFKEFSNVTDEMIAQERNKHKPAILQNIETFIKRSQLRNLPRTPNISQEYLSNIYDRFYKCIQSHKASLGTGSSSMEFQTFQQFMAGFCDWVLSDEEYDSHPDDFLHRLFRNWDKDHKGVLTLTDVVVGLDKLVNRNLMESISNFFEIYDPESKGEVDREGILQLSEGLLYITKPLQDGLVFDYLTRKSIESYIADRIVERQKETNDEEIMLPSEVNIDQEIFQREQGERYLSAASEFIQRAFEYAQPVTQPQLIELEDSKDLDKMKANAALDPNHPLTLNLATFRMVILADETYELLFTETLASSIHLDRSTSISDKGGILRDMFDGLLADGRRVANQVRRRMDSTATSISNNNGHSDSAGVKSKTGAASIAEEEDEDDFGGDATTHDYGDLLEGAEIQALGDVPLSSNVKETDRANELKVLHDNMDEIKRTLDGQESSKNDNLIEFES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0E4</accession>
    <name>A0A1E3P0E4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP cyclohydrolase 1</fullName>
            <ecNumber evidence="3">3.5.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GTP cyclohydrolase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_33219</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">GTP cyclohydrolase 1 is the first enzyme in the biosynthetic pathway leading to folic acid.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="3"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037937.1">
        <property type="nucleotide sequence ID" value="XM_019182045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0E4"/>
    <dbReference type="STRING" id="683960.A0A1E3P0E4"/>
    <dbReference type="GeneID" id="30199291"/>
    <dbReference type="OrthoDB" id="4966at2759"/>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00642">
        <property type="entry name" value="GTP_cyclohydro1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.286.10:FF:000003">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1130.10:FF:000012">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00223">
        <property type="entry name" value="FolE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018234">
        <property type="entry name" value="GTP_CycHdrlase_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00063">
        <property type="entry name" value="folE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006825">
        <property type="entry name" value="PRK09347.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006826">
        <property type="entry name" value="PRK09347.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109:SF7">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="GTP CYCLOHYDROLASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00859">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00860">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GTP cyclohydrolase I" evidence="13">
        <location>
            <begin position="130"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ58730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="309" mass="34319" checksum="ADCA6CEF6188C9CC" modified="2017-01-18" version="1">MSDKLARDISELNLSPPSTSSSSSLPPNAHVNGAGYSTTAHTSISDHHHHHNTQPSSSSSSSSSPTTHHPKPSTRPAPRAIDSPLAIRPASPYTLNPPIDSDGLSWPSVGARKRIDSTPEEAELREKRISDAVKVILEELGEDTNREGLLETPERYARAMLYFTKGYEDNIRDVIKRAVFEEDHDEMVIVRDIEIYSLCEHHLVPFFGKVHIGYIPNKKVLGLSKLARLAEMYARRFQVQERLTKQIAMALKEILKPQGVAVVIEATHMCMVSRGIQKTGSSTVTSCMLGCFREAQKTREEFLGLLGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0G2</accession>
    <name>A0A1E3P0G2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UV excision repair protein RAD23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_34701</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Involved in nucleotide excision repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RAD23 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037594.1">
        <property type="nucleotide sequence ID" value="XM_019182218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0G2"/>
    <dbReference type="STRING" id="683960.A0A1E3P0G2"/>
    <dbReference type="GeneID" id="30199464"/>
    <dbReference type="OrthoDB" id="419317at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000111">
        <property type="term" value="C:nucleotide-excision repair factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120125">
        <property type="term" value="C:PNGase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000224">
        <property type="term" value="F:peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14281">
        <property type="entry name" value="UBA2_Rad23_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01805">
        <property type="entry name" value="Ubl_Rad23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000002">
        <property type="entry name" value="UV excision repair protein RAD23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000003">
        <property type="entry name" value="UV excision repair protein RAD23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.540">
        <property type="entry name" value="XPC-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004806">
        <property type="entry name" value="Rad23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015360">
        <property type="entry name" value="XPC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036353">
        <property type="entry name" value="XPC-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00601">
        <property type="entry name" value="rad23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621">
        <property type="entry name" value="UV EXCISION REPAIR PROTEIN RAD23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621:SF0">
        <property type="entry name" value="UV EXCISION REPAIR PROTEIN RAD23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09280">
        <property type="entry name" value="XPC-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01839">
        <property type="entry name" value="RAD23PROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101238">
        <property type="entry name" value="XPC-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3 5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="7">
        <location>
            <begin position="139"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="7">
        <location>
            <begin position="341"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="79"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="187"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="79"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="187"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="385" mass="40973" checksum="304473339CA7D088" modified="2017-01-18" version="1">MQIIFKDFKKEKIPIEVEPTETVLQAKEKLAEIKKVEVKQLKFVYSGKVLQDDKTIESTKIKADDQVIFMISVIKKSTPTPAATSTQSSTPIPAQSSEPAATSQPSAAPSQAPSAAPVGASSTEAQAGDFDASTFATGSARERAINNIMEMGYERPQVEQALRAAFNNPDRAVEYLLTGIPAEFQQQAQAPVAPQEGQPQAPTAVEEAGEEPTTTTTAAPAAPEAQEAEEHQSNEHGDLFAQAAAAGGNTPSRPAGGAPQGAGLDQIREIVRTNPEMLEPLLEQLGQQYPQLNGLIQQNPEEFINMILNGLGDEEVGAAGAGLEGEAAGGDDGRVEIPITEEDQAAINRLVELGFESDLVIQVYFACDKNEELAANILFNDHAEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0H5</accession>
    <name>A0A1E3P0H5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_64560</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037629.1">
        <property type="nucleotide sequence ID" value="XM_019183761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0H5"/>
    <dbReference type="STRING" id="683960.A0A1E3P0H5"/>
    <dbReference type="GeneID" id="30201007"/>
    <dbReference type="OrthoDB" id="4676at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016813">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03884">
        <property type="entry name" value="M20_bAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010158">
        <property type="entry name" value="Amidase_Cbmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01879">
        <property type="entry name" value="hydantase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006771">
        <property type="entry name" value="PRK09290.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494:SF5">
        <property type="entry name" value="ALLANTOATE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494">
        <property type="entry name" value="ALLANTOATE DEIMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001235">
        <property type="entry name" value="Amidase_carbamoylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="3">
        <location>
            <begin position="243"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="445" mass="49195" checksum="817A5B50CC4466AD" modified="2017-01-18" version="1">MSTITETAATTLKLSGNPVRALKVKPNRLINTIHETAKFGAKFKWGPLETDTGVCRLALSDEDKKVKDWFVNEVKSLGCKVKIDQIGNIFAIFPGQNNDAKPTAMGSHLDTQPTGGRYDGIYGVLSGLEVLRTLKDNNYVPKYPIVLINWMNEEGARFPLSIMASSVWSGAFKLEDIYKVESVTDETPVTVEHELKRIGYLGDTPASYEAMPLAAHFEIHIEQGPILEAENKKIGVVEGVQAYTWWRIKVTGKAQHTGTTPLALRNDPLLATSQMIVKANEIAKKHDGLASIGILNLGPGVVNVIPDEVSFALDMRNVKDEVLNKMIEEFKKEFTEITKATGSKINVEFEHLHTQPATHFNKTCIKCVEDSALELFGADQYRKITSGAGHDSCSTATRCPTSMIFIPSKDGVSHNPEEYSTPEQVDEGFRVLLSAVLKYDQLRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0Y4</accession>
    <name>A0A1E3P0Y4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gfd2/YDR514C-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_43967</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038127.1">
        <property type="nucleotide sequence ID" value="XM_019182580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0Y4"/>
    <dbReference type="STRING" id="683960.A0A1E3P0Y4"/>
    <dbReference type="GeneID" id="30199826"/>
    <dbReference type="OrthoDB" id="5953249at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040151">
        <property type="entry name" value="Gfd2/YDR514C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048519">
        <property type="entry name" value="Gfd2/YDR514C-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28083">
        <property type="entry name" value="GOOD FOR FULL DBP5 ACTIVITY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28083:SF1">
        <property type="entry name" value="GOOD FOR FULL DBP5 ACTIVITY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21762">
        <property type="entry name" value="DEDDh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfd2/YDR514C-like C-terminal" evidence="1">
        <location>
            <begin position="44"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="229" mass="25578" checksum="59FA2394F9257DEB" modified="2017-01-18" version="1" fragment="single">MGETEKYIKELDSSRKKHPILQDKGKFNIITDALRSVNGQKTTLISIDLEAFERNTEIVTEIGISIYDPVKEATSLVPSIINIHLILKETIKLRNGAFVHDHKDNFLGGSSLVVSKKTAVDLIESVLEFYCVQRKAQGYEAAFVGHNLSGDLNWLKKLGVHLPQNPHILDTEKVYSASHGSQGNSLGAILKRLRIPHSFLHNAGNDAYFTLIALLKLSDPHCRRTEKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1C4</accession>
    <name>A0A1E3P1C4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peroxin/Ferlin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_55818</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038458.1">
        <property type="nucleotide sequence ID" value="XM_019182764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1C4"/>
    <dbReference type="STRING" id="683960.A0A1E3P1C4"/>
    <dbReference type="GeneID" id="30200010"/>
    <dbReference type="OrthoDB" id="72441at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006614">
        <property type="entry name" value="Peroxin/Ferlin"/>
    </dbReference>
    <dbReference type="SMART" id="SM00694">
        <property type="entry name" value="DysFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peroxin/Ferlin" evidence="1">
        <location>
            <begin position="145"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="182" mass="21709" checksum="A420FA83FBA2C9B3" modified="2017-01-18" version="1">MTISLISNDQDQIYQDPEISFEDKNNKPSIITNYIIPKYKLDQIVSNCIENLSKQKTISSTTTSTTSTSSLEYSYDLLIENERGFIIFGLPFYTKYMFPFDPPNFTNINGSKISNLKLFPLPDLNWKWSWDKWYVIMNDDNDDQGWCYSWRFGSKHWRGEQRLGCFVRRRIWLRLRERKILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P258</accession>
    <name>A0A1E3P258_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">OBG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_79804</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038491.1">
        <property type="nucleotide sequence ID" value="XM_019184953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P258"/>
    <dbReference type="STRING" id="683960.A0A1E3P258"/>
    <dbReference type="GeneID" id="30202199"/>
    <dbReference type="OrthoDB" id="545683at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04938">
        <property type="entry name" value="TGS_Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01899">
        <property type="entry name" value="Ygr210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.470:FF:000001">
        <property type="entry name" value="GTP-binding protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013646">
        <property type="entry name" value="YGR210-like_G4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF1">
        <property type="entry name" value="OBG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08438">
        <property type="entry name" value="YGR210-like_G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="3">
        <location>
            <begin position="5"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="414" mass="45210" checksum="1F88BB627F3AC852" modified="2017-01-18" version="1">MPRDPLIGIVGKPSSGKSTTLNSLTDAAVKTGAFPFTTIDPNKATGYLKVDCACSRFGKEALCKPNYGWCSNGSRHIPILLLDVAGLVPGAHMGRGIGNKFLDDLRQADALIHVVDVSGSTDAEGKATLGYDPLADVEWLQDEIRLWIEGNLEKRWGSIVRRHTATKSSVVDTLLTQFAGYSANGQMIARAMDRVPDVGKLEDWNKEDITRVVKAFMDEKFPTVLSLNKIDHPDSDKNVSKILLKYPNTKAVLTSAITEVFLRKLSKQGFIKYEEGTEFIDTREDLPDDESLKPMDEKLLNRVENIRDLVLYRFGSTGVVQVLQAATEVLGLVPIYTVRNISNFTGSNGTNVFRDCTLIRKGTPVSKVARHIMGEVTIAAIEGVGGVRVSEDDTIDVGKNDILSFRVVPGGGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P259</accession>
    <name>A0A1E3P259_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_62137</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038758.1">
        <property type="nucleotide sequence ID" value="XM_019183313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P259"/>
    <dbReference type="STRING" id="683960.A0A1E3P259"/>
    <dbReference type="GeneID" id="30200559"/>
    <dbReference type="OrthoDB" id="18320at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006722">
        <property type="entry name" value="Sedlin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04628">
        <property type="entry name" value="Sedlin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="20760" checksum="61C507371921DDF5" modified="2017-01-18" version="1">MPKHIFFVSLISRDNRPLHIQSLTPFQGEESIISERVDDQTKPVVEGDAKSQKSGETHTLVDELSENEILKYQFLSNMALDIFLSPFFEGEQGIPVLLFIQDGINIYGYETNTGLKILIGSSSEIDESFGFIFKKLHRLYLNLIVNPFQSNDLSQLTINEEKTTLKVNQIIDEWDKKYNTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P292</accession>
    <name>A0A1E3P292_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Protein PBN1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_106231</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for proper folding and/or the stability of a subset of proteins in the endoplasmic reticulum. Component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase GPI14.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endoplasmic reticulum membrane</location>
            <topology evidence="11">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type III membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the PIGX family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038670.1">
        <property type="nucleotide sequence ID" value="XM_019180466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P292"/>
    <dbReference type="STRING" id="683960.A0A1E3P292"/>
    <dbReference type="GeneID" id="30197712"/>
    <dbReference type="OrthoDB" id="5546453at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990529">
        <property type="term" value="C:glycosylphosphatidylinositol-mannosyltransferase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042322">
        <property type="entry name" value="Pbn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013233">
        <property type="entry name" value="PIG-X/PBN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28533">
        <property type="entry name" value="PROTEIN PBN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28533:SF1">
        <property type="entry name" value="PROTEIN PBN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08320">
        <property type="entry name" value="PIG-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00780">
        <property type="entry name" value="PIG-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 11" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ59463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="453" mass="52153" checksum="2B9C72EC7F35542E" modified="2017-01-18" version="1">MIKQRITLLSNYIEQEADTEGLEFSEELVTVPKSIYYSRQDRVTIPIEHITPDLAQQLNTLGKLRIQWKAAGAHPSEIFQNFNQQGLYIHLVPSTSYYQESIDILSQQLSTSLGLEPFDNSDFIVTATSATYYSNSSIENIKLTQFLSSLTQLDQQDINNIEDFELNWDKESGLKLQWVSSPSDFKISKSTEIRKELAILQPKDLVPDIELAGIRTVLGQDYLPPTKTLIYIKPRHFTTKNTIHYELQSPIGLHPTLKLSDLNYTPPSENCKLFWVSTVDNRLIFDKYQYDEKKFKLLQSWGEIDLEAPVWKVSKFGSIQFLEVLNKTNLEMTYHSRYIEPSSDGKTVLLTPNVFYACDSPELIEDHQLIEKNPFDSYGLGYESFFDKDTIFFHFENEPKTLEFNIPTAKVEDFNRIQLTTLSVVSIGTLYLLYKLFRTFTTNTPVSSSKKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P293</accession>
    <name>A0A1E3P293_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_84689</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038224.1">
        <property type="nucleotide sequence ID" value="XM_019185756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P293"/>
    <dbReference type="STRING" id="683960.A0A1E3P293"/>
    <dbReference type="GeneID" id="30203002"/>
    <dbReference type="OrthoDB" id="3979216at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000013">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF101">
        <property type="entry name" value="HIGH-AFFINITY NICOTINIC ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="348" mass="38912" checksum="1A1B752C94960211" modified="2017-01-18" version="1">MLLGLFESGIIPVINVYLGMLYTKTEMAKRCSAVYCASAAAGGFGGLLAFGLIKINAHGWEGWRWLFAIEGALTICSFPILIILLPRDPTTVWWLNADERKVLVTRLTKYPDFYQDEKFAWSEVKRSLIDVNNILVYLYEFCVDLTLFGISTFLPSIIKGMGYNKYETQLLTIPFYAIALISFIIVGYFSDRFQHRGGFLIIGLIFEIIGYVILVTADSLGARYFACMMIGVGIYVCSALSIMWINNNNAGHYKRATAGGMITTIGSTAGVLAGQIYTAKSAPRYFKGLRIALALTCAAVFFVIAMMYNYNRLNNKRAKKVQELLKEGVDISDEKPKDDTSLNFKFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2D9</accession>
    <name>A0A1E3P2D9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit delta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit delta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_20455</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038853.1">
        <property type="nucleotide sequence ID" value="XM_019180872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2D9"/>
    <dbReference type="STRING" id="683960.A0A1E3P2D9"/>
    <dbReference type="GeneID" id="30198118"/>
    <dbReference type="OrthoDB" id="10254737at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002183">
        <property type="term" value="P:cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10233">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10233:SF14">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="370" mass="41802" checksum="D68D5AE2ADE8C336" modified="2017-01-18" version="1" fragment="single">QNLIHPAILSLTSKYSSYSIVGSIPRCIAMLKAFKTVISEYQTPEGTTLTRNLTNYLGYQIDYLKTSRPLSVTMGNAIRWLKQEISLISIDTSDIDAKNDLVEKIDIFLKEKIELSDKLIIQSASNHIQNGSTVLTFGNSNVLKELFLYNAKELQKKFRVIIVDSRPLFEGKKLAKILTLNGLKVQYALINSITSIFQDVDTVFLGAHAMLSNGFLYSRVGAALIAMTAKKRNIPVLVCCESIKFSDRVQLDSVTLNELGDNDDLIDCNQLNHVKKSSVALQNFIETHEDEQKQKDQQQQQKKNNKNDKEKENSIVQNELPLKDWKKSSFLNILNIMYDLTPPEYIQKIITEVGALPPSSVPVILREYKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2U2</accession>
    <name>A0A1E3P2U2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ureidoglycolate lyase</fullName>
            <ecNumber evidence="3">4.3.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Ureidoglycolatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_30379</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the catabolism of the allantoin degradation intermediate (S)-ureidoglycolate, generating urea and glyoxylate. Involved in the utilization of allantoin as secondary nitrogen source when primary sources are limiting.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(S)-ureidoglycolate = urea + glyoxylate</text>
            <dbReference type="Rhea" id="RHEA:11304"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57296"/>
            <dbReference type="EC" id="4.3.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nitrogen metabolism; (S)-allantoin degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ureidoglycolate lyase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.3" evidence="3"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039013.1">
        <property type="nucleotide sequence ID" value="XM_019181728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2U2"/>
    <dbReference type="STRING" id="683960.A0A1E3P2U2"/>
    <dbReference type="GeneID" id="30198974"/>
    <dbReference type="OrthoDB" id="10266039at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004848">
        <property type="term" value="F:ureidoglycolate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050385">
        <property type="term" value="F:ureidoglycolate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000256">
        <property type="term" value="P:allantoin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20298">
        <property type="entry name" value="cupin_UAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.480:FF:000003">
        <property type="entry name" value="Ureidoglycolate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.480">
        <property type="entry name" value="Ureidoglycolate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047233">
        <property type="entry name" value="UAH_cupin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007247">
        <property type="entry name" value="Ureidogly_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024060">
        <property type="entry name" value="Ureidoglycolate_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21221">
        <property type="entry name" value="UREIDOGLYCOLATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21221:SF1">
        <property type="entry name" value="UREIDOGLYCOLATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04115">
        <property type="entry name" value="Ureidogly_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="213" mass="23425" checksum="3D65A1635BF84425" modified="2017-01-18" version="1">MVLKTYDTTGKKAVVTKQLTPKSFAKFGDIISADHQIGLKEQSSANYGTAVKVHKVAKVINNSSNAKSGVKAQANWNIFRCSPPNHLFQKNSDGNIVYLSKVLERHPYSTQTFLPMGVDKEKDAYIVICAKSRKGIIEDSLPDPDTLEAFICKGNQAVTYGAGTWHAPMVSLVDNLDFGVLIHENGIADEDCQECYFEPGYNVVIKSHLSSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2X1</accession>
    <name>A0A1E3P2X1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Aspartate protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_29757</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038900.1">
        <property type="nucleotide sequence ID" value="XM_019181644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2X1"/>
    <dbReference type="STRING" id="683960.A0A1E3P2X1"/>
    <dbReference type="GeneID" id="30198890"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097718">
        <property type="term" value="F:disordered domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070492">
        <property type="term" value="F:oligosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016237">
        <property type="term" value="P:microautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000425">
        <property type="term" value="P:pexophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05488">
        <property type="entry name" value="Proteinase_A_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000036">
        <property type="entry name" value="Vacuolar aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000002">
        <property type="entry name" value="Vacuolar aspartic proteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033819">
        <property type="entry name" value="Saccharopepsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="8 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="Peptidase A1" evidence="14">
        <location>
            <begin position="56"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="87"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="292"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ59693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="369" mass="40114" checksum="D04441E9770F841C" modified="2017-01-18" version="1" fragment="single">KRPIDDQLKDATFDEHVRQMGQKYLGLFQKAYPESDVPFIDGTHETPLTNYLNAQYYTEIQLGTPGQPFKVILDTGSSNLWVPSTDCSSLACYLHSKFDHEASSTYKANGSDFAIRYGSGSLEGYVSQDTLQLGDLIIPKQDFAEATSEPGLAFAFGKFDGILGLAYDTISVNKIVPPVYQAINAGLFDEPKFAFYLGDSDKSEDGGVATFGGVDESKYSGKVTWLPVRRKAYWEVKFNGIGLGDEFAELENTGAAIDTGTSLIALPSGLAEILNAEIGAKKGWSGQYSVDCETRDSLPDLTFNLDGYNFTIGPYDYTLEVSGSCISAFTPLDFPAPIGPLAILGDSFLRKYYSIYDLGNDAVGLAKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2X9</accession>
    <name>A0A1E3P2X9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_63731</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038444.1">
        <property type="nucleotide sequence ID" value="XM_019183601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2X9"/>
    <dbReference type="GeneID" id="30200847"/>
    <dbReference type="OrthoDB" id="10632947at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133638" evidence="1">
        <location>
            <begin position="17"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="337" mass="38563" checksum="45D82BE16391B57E" modified="2017-01-18" version="1">MKLSLLLSTFTVSCTAISSVSRICDPGRIARPNFTGFRFDFQTSFNSDVSYIARSYHLVLCKEGHPISDIRYELDPADAKIPWDKTLCTADRGNCYKLARSKKTMLKARPFMSTFPGETVGGLFELSDSQAEVAKDESYLPVSRLLQDSLTSMWFEIHLNRDLFTAHFPLFHTFDPNSFELDSDFFLRGADQWESFISANEDYKFEPQDRTLMEKANEQVNSKISQVINDSNKKLSSIVSRFQKKLSTIANSVLNVQEVVKKRKATLFPKIFKSKIETLSKLKGKLNPNEIDLDTMIVLSKVLPKEKFEELLKSIDQVYANVPLKKEIFKYPAQKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P321</accession>
    <name>A0A1E3P321_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein RMD9, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_83813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038950.1">
        <property type="nucleotide sequence ID" value="XM_019185602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P321"/>
    <dbReference type="STRING" id="683960.A0A1E3P321"/>
    <dbReference type="GeneID" id="30202848"/>
    <dbReference type="OrthoDB" id="4081443at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="567" mass="64489" checksum="3711AA75928F578E" modified="2017-01-18" version="1">MFRLTAQSNVLKSRIANQVVSNARNSLNHSIRFNSVALERSASSEINNNNNNNGNNSSFNFSKTIKDVASSQNQQKEKNNAFKSYRSSNNSSSSPWFNQLNAFEDCVTQTADFNSSGSQPFFWENAQKAMNLYHELRGTPDFTPAAIGKLCHLLHSGLRANRFQLTRLSKKPDYDSKSFHTEMQNFVKYSVREIINDLLEQRVSVNENGAMHLLTSLKELKLDQEAVAVWSGAMKVETLKDTFLQPRVVGVLLPLMFEAGTPFEDVNALFQESRKATVQLHQNLAIGMIKTCLAANQDNQALTLFTELCQDAKHGKVPYMIEAHLTFIGDCKDLNIAGSFFEKAINREMPYRLNLQVNSVKSYLNNIWEETGDFNRVVDAWTKATTFYGRNIHHGISSSLNNMFFEIFFKKYEGDRIAGLAHLKDIIAVYNDIKPIDEPFFNIIISKCGVWGDKETIDSLYKAYDIYRVKKTQISRRIYLKVLGSVNSTEEEILSSWYDMLRFADYEGSTYIANADWAAIRDATVNSKTNQRNLLYYSANGSLMDLAQIDDSNVQLVDFTSIRRTHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P341</accession>
    <name>A0A1E3P341_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Electron transfer flavoprotein-ubiquinone oxidoreductase</fullName>
            <shortName evidence="19">ETF-QO</shortName>
            <ecNumber evidence="19">1.5.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">WICANDRAFT_79830</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 19">Accepts electrons from ETF and reduces ubiquinone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>a ubiquinone + reduced [electron-transfer flavoprotein] = a ubiquinol + oxidized [electron-transfer flavoprotein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24052"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.5.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="19">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ETF-QO/FixC family.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.1" evidence="19"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038519.1">
        <property type="nucleotide sequence ID" value="XM_019184957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P341"/>
    <dbReference type="STRING" id="683960.A0A1E3P341"/>
    <dbReference type="GeneID" id="30202203"/>
    <dbReference type="OrthoDB" id="437331at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000015">
        <property type="entry name" value="Electron transfer flavoprotein-ubiquinone oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040156">
        <property type="entry name" value="ETF-QO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049398">
        <property type="entry name" value="ETF-QO/FixC_UQ-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007859">
        <property type="entry name" value="ETF-QO/FixX_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10617">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-UBIQUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10617:SF107">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-UBIQUINONE OXIDOREDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21162">
        <property type="entry name" value="ETFQO_UQ-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05187">
        <property type="entry name" value="Fer4_ETF_QO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9 19" id="KW-0274">FAD</keyword>
    <keyword evidence="6 19" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 19" id="KW-0408">Iron</keyword>
    <keyword evidence="14 19" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 19" id="KW-0813">Transport</keyword>
    <keyword evidence="15 19" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="20">
        <location>
            <begin position="551"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00052682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU366068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ59312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="591" mass="66214" checksum="DF5D65303011230D" modified="2017-01-18" version="1">MDDEMKELLTEERAADKVDVCIVGGGPAGLATAIKLKQLELENESREIRIVVLEKAPDMGSHIVSGAVLEPRALRELFPESEYYDENGEGIPLPSDIATPVTDETLRYLYHDWAVPMPEPPQMINKGKNYIVSLSEVVKHLAEKAEELGIEVYPSTAVSEVLYDSTGAVKGVATKDMGISKDGTPKDSFERGMEFHARVTVFAEGCHGSLSKEVINKFNLREGRDNQTYGLGIKEVWEVEPSKFKKGFIGHTMGYPLSYDVYGGGFMYHFGDGLVSVGIVVGLDYENPWISPYQEFQKMKHHPFYSDVLEGGKCISYAARALNEGGWQSVPKLNFPGGVLVGASAGFMNVPKIKGTHTAMKTGMLAAENIFEVVSKLEKIKTEEDEDYDPEIHDNIEETPINLESYEQAYKRSWVYDELYEVRNIRPSFGTGLKLFGGVSYSGIDSLFLKGQVAWTFHHHKSDAHITRPASEYEKIEYPKPDGKISFDILTSVSRTGTYHDENEKNHLRVPDQDLDKHRDISYPKFKGVENRFCPAGVYEYVEDEKTEHGVKFQINSQNCIHCKTCDIKVPTQDINWTVPEGGDGPKYVMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P346</accession>
    <name>A0A1E3P346_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_62480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039106.1">
        <property type="nucleotide sequence ID" value="XM_019183379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P346"/>
    <dbReference type="GeneID" id="30200625"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133657" evidence="1">
        <location>
            <begin position="19"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="80" mass="8919" checksum="362E4F8D6F3570EE" modified="2017-01-18" version="1">MKFSSVLAFTFSLGVTLAAGPINIYVTKYNTIEVVETATTTVTQNSSQLLEFGIQMNKLEFESPIIDCLQHLVRYWIAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3B8</accession>
    <name>A0A1E3P3B8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TBP-associated factor 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Transcription initiation factor TFIID subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_31215</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038957.1">
        <property type="nucleotide sequence ID" value="XM_019181809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3B8"/>
    <dbReference type="STRING" id="683960.A0A1E3P3B8"/>
    <dbReference type="GeneID" id="30199055"/>
    <dbReference type="OrthoDB" id="361039at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000144">
        <property type="term" value="P:positive regulation of DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22931">
        <property type="entry name" value="HFD_TAF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08050">
        <property type="entry name" value="TAF6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000033">
        <property type="entry name" value="Transcription initiation factor TFIID complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.770:FF:000003">
        <property type="entry name" value="Transcription initiation factor TFIID complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.770">
        <property type="entry name" value="TAF6, C-terminal HEAT repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037796">
        <property type="entry name" value="TAF6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011442">
        <property type="entry name" value="TAF6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046344">
        <property type="entry name" value="TAF6_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004823">
        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10221">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10221:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02969">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07571">
        <property type="entry name" value="TAF6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00803">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TATA box binding protein associated factor (TAF) histone-like fold" evidence="9">
        <location>
            <begin position="5"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="165"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00076308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="471" mass="52836" checksum="FD14149A7A257DA8" modified="2017-01-18" version="1">MSHTLWSPIDTVKDVADSLGLTDLNDEASRNLAMDVEYRIHEILEQAVKFMRHSKRRTLTTTDVEKALRVLNVEPLYGYDVSRPLNFKEALVGNGQTLYYIDDEEVDFEKLINQPLPKVPRSTTFTAHWLAIEGVQPAIPQNPLESEIRSLLPASRGAITNVLNNNDSAVTSNNNTTNGHSTNNNSTSGTSSKDTEIKPLVKHVLSKELQLYFDKVIQALTNQEDTEEALHFKQAALTSLRSDPGLHQLVPYFVQLISEQITHNLNNIALLSTMLEVIYSLLSNDNIFLDPYIHALMPCILTLLLAKRIGSNNDDEHFAVRDFASSLLEHVCKNYGKAYTTLKPRVTRTLLKAFLDSNKPVGTLYGAIIGLKNLGDEVIRIIILGNLQNWSLIVLDSLKNDEKSLLIGTIIKALRSLIPENAPDDEMDVEIIEDDKQKLQKRVGDELANEIIKQDDSKKLIDAIFFGDLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3C9</accession>
    <name>A0A1E3P3C9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_91749</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ59818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039025.1">
        <property type="nucleotide sequence ID" value="XM_019186318.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="30203564"/>
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    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018809">
        <property type="entry name" value="DUF2406"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28186">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28186:SF1">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10295">
        <property type="entry name" value="DUF2406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="231" mass="26149" checksum="8D9C89EB7C6C8CF7" modified="2017-01-18" version="1">MGLFRKNSNASQHSGGSGGKPAKLQITKDDERAYKVRTGSVHDPILQAVNEAQPFEEAALDQQLEGNSNSSLTRQSYNMMASNGGFYDVFGKPIHQPDISNPTRARDERPLDTIRGFEYSITGDLSYKDRLESDNLGFRVRNDFPSFAGGNPYGAGAAAVFLEINKLLNKVFMKPLLKKLKLLKRRRDFSVERRNEELHGTILTIFERMVLLYDYYEYLGRKDMIVTLAGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3I7</accession>
    <name>A0A1E3P3I7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_83927</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039120.1">
        <property type="nucleotide sequence ID" value="XM_019185623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3I7"/>
    <dbReference type="GeneID" id="30202869"/>
    <dbReference type="OrthoDB" id="3980057at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="224" mass="26388" checksum="14460ABFCA45129D" modified="2017-01-18" version="1">MSYQVLGFQLFNLNDYGIKLFPAFKEDHLYFAKFQILEKYPDQDTHDEGSSQRSDKLLLNPNAKSKELLKNFPFTALLREELVQVKTTKQQLQNAIILFNFEYNYILILYLNTQDSADKLTKYFASLDLNVEETETIAQFIKKKQDELDEKIYKESLGKDRLQKKVNPKHSKEIPLLANHINIIEDDDDLKDLYLKLIQQFDNEFLHSESANFEYDSTTQKNIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3K1</accession>
    <name>A0A1E3P3K1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_16896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RutC family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038991.1">
        <property type="nucleotide sequence ID" value="XM_019180716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3K1"/>
    <dbReference type="STRING" id="683960.A0A1E3P3K1"/>
    <dbReference type="GeneID" id="30197962"/>
    <dbReference type="OrthoDB" id="309640at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00448">
        <property type="entry name" value="YjgF_YER057c_UK114_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="2-IMINOBUTANOATE/2-IMINOPROPANOATE DEAMINASE RIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803:SF58">
        <property type="entry name" value="PROTEIN HMF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="113" mass="12173" checksum="878DFDF06388FDA3" modified="2017-01-18" version="1" fragment="single">FLSPAYKSNGHVFCSGSVGTDAAGKIPTDVKLQTEIAIKNLEGVLKASGSSLDRVLKVLLFVAHPADAAKVNEVYAKYFTTKPSRSCIVVGFPNPEIKVELECVAEYKEIKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3U2</accession>
    <name>A0A1E3P3U2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_100346</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DEAH subfamily. PRP16 sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039292.1">
        <property type="nucleotide sequence ID" value="XM_019180291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3U2"/>
    <dbReference type="STRING" id="683960.A0A1E3P3U2"/>
    <dbReference type="GeneID" id="30197537"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034458">
        <property type="term" value="F:3'-5' RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000386">
        <property type="term" value="F:second spliceosomal transesterification activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000378">
        <property type="term" value="P:RNA exon ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040031">
        <property type="term" value="P:snRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000615">
        <property type="entry name" value="pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000018">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase prp16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF91">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE PRP16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="177"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="354"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="807"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="126"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="807"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="819"/>
            <end position="828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ60085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="844" mass="95027" checksum="3BDB8F3AFF65EDF6" modified="2017-01-18" version="1">MSLFKKRKDHRQRDNDIWEKNRLQTAGTIQKGDLDLDFEDTSEPTVQLSTHHLTPPFLTGTNVFTTQRDPISPVRDPQSDIAIAAKRGSALVQQRRQQRERKSKAKDAASLVGSVIGEVLGVDQQKNQSDVNKNSKFNNLPKENGNGDDVEAEIGTKLSIEEQRKTLPAYAARDDVLKMIRDNQVVIVIGETGSGKTTQLTQFLNEDGYGRLGMIGCTQPRRVAAVSVANRVAEEMNVKVGEEVGYVVRFEDVTSPKTIIKYMTDGILLRETLVDSDLEKYSCIIMDEAHERTLSTDVLMGLFKNLLARRRNLKLIITSATMNADRFSRFFGDAPQFTIPGRTFPVDIMFSKFTVEDYVESAVKQALTIHLQSGPGDILIFMTGQEDVEVTCEVLADRLKQLDDPPPLEILPIYASLPAEQQQKIFKKTKSGYRKVVVATNIAETSLTVDGISFVIDPGYSKLKVYNARIGLESLAITPISVANANQRAGRAGRTGPGSCYRLYTEKAAREEMYPQTVPEIQRTNLANTLLLLKSLGVDDLIKFPFLDPPPKETITASLYELWSIGALDNFGKLTPLGRQMAQFPIQPALSKLLLIASQNGCSEEMVIIVSMLSVPSVFYRPKERQEESDLSRSRFFVPESDHLTLLNVYSQWKANNFSDFWCKKHFLHSKSLKKAKDIKEQIELIMKSNKVPVTSSGYEWDVVRKCICSGYFYQAAKVHGFGEFVNLRTGMKLQLHPTSALFGMSDLPQYVVYHELMLTTKEYISTVTAVDPLWLVEYGGVFYSVRDKKNKDNGFKKRKIEEELERDAKSYEEEKSKAKLKTTKKSKPVVNIGGNLNRRRRGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P491</accession>
    <name>A0A1E3P491_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Manganese transporter SMF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_29981</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039277.1">
        <property type="nucleotide sequence ID" value="XM_019181674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P491"/>
    <dbReference type="STRING" id="683960.A0A1E3P491"/>
    <dbReference type="GeneID" id="30198920"/>
    <dbReference type="OrthoDB" id="409173at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015086">
        <property type="term" value="F:cadmium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015295">
        <property type="term" value="F:solute:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00221">
        <property type="entry name" value="NRAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01197">
        <property type="entry name" value="nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037982">
        <property type="entry name" value="Nramp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF101">
        <property type="entry name" value="MANGANESE TRANSPORTER SMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="336"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="427"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="451"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="514"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="546" mass="59845" checksum="D3435D0CD2611EE9" modified="2017-01-18" version="1">MAGGVRDHNNLSRTHSETNNKSKDIYEITNTLSEDKNSIKQPTKRIDTIGRKFTKFFQVKENDAKIVKIFKKYLRFVGPGLMISVAYMDPGNYATDVSAGASSQFSLLFIVLLSNIIAIFLQSLCIKLGSVTGLDLSRACRDHLPKWLNLIIWFFAEAAIIATDVAEVIGTAIALNILLRIPLPAGVVITCIDVLFVLMAYRPGSSLKFVRLFEYAVALLVLCVVVCFCVELGLMPPVNVRSVFRGFAPSREMFENNGMYTACSILGATVMPHSLFLGSGLVQPRLREFDVENGYVKLPSSDDEATVVEDQEDALFKYRPTAKAIKYAMKYSIAELAITLFTFALFVNSAILIVAGSSLHGTPEAVDADLYTIHKLLSHQVAPIAGTIFMLALLFSGQSAGIVCTIAGQIVSEGHLHWTMKPWKRRIVTRSISIVPCLAISLSIGKSGLSAALNASQVVLSILLPFLTAPLIYFTCKKSIMRVEVNQADEDNTNDAVGTSNELGTKYIDMSNNWATSIIAFLIWLFISVLNVYTIVKLGLSHGDIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P497</accession>
    <name>A0A1E3P497_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_62890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039533.1">
        <property type="nucleotide sequence ID" value="XM_019183456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P497"/>
    <dbReference type="GeneID" id="30200702"/>
    <dbReference type="OrthoDB" id="3982608at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="258"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="294"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="385"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="441" mass="49929" checksum="64E2775E673D99CF" modified="2017-01-18" version="1">MALAVKQFKFCREFTSQPADAQEQQGVNITLLQDSTVLLFGLKDLPDTNIPLLYDENFNLKENRDGFVLCTEELVASGECSKIGEFRIKKTADLQQPLLNMFGEKGDQFFYPVNNSGVYCLYGHHSSEEPGVEYRFYKPYGSLTSSDAKKWKFVTFVAIPLAIILLLGFGAKFLFTKRKGDKTSLVFGSVDAACASIAFNDEGYNSPQGVGFFGLVSDRVFSEYFLSIMHLLVYSVLLEFSKGGYGTVYDQSVKRTRAYGYVCLAILFANVWVQSGSYGLYEKFDLLMNLINNFGYYVIWGFIFQYSKITISEITNESTKRKFIRSRRLTIFTPVFLSLISGFSMIALSFNASQNFVKEHPEIDPVKDMDKVFIHTMENSNSRSIILAVLENLPSLSFFIIILGYSAIWQTTKNDEKLASKDNTMSDPDTENLLVDFKEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4F1</accession>
    <name>A0A1E3P4F1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">26S proteasome regulatory subunit 6B homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_84090</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039347.1">
        <property type="nucleotide sequence ID" value="XM_019185646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4F1"/>
    <dbReference type="STRING" id="683960.A0A1E3P4F1"/>
    <dbReference type="GeneID" id="30202892"/>
    <dbReference type="OrthoDB" id="10255768at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045899">
        <property type="term" value="P:positive regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000020">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000046">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000033">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="194"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="33"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ60140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="417" mass="46650" checksum="79E7418E294FE33F" modified="2017-01-18" version="1">MEELGIQPTAAPSFDKSKIENLFINTTNQSSNNADIYLKLKKLEKEYELLTLQEDYIKDEQRHLKRELVRAQEEVKRIQSVPLVIGQFLEPIDQNVGIVSSTTGSNYVVRILSTLDRELLKASASVALHRHSNALVDILPPEADSSIAILGDGEKPDVTYADVGGLDMQKQEIREAVELPLTQGDLYEQIGIDPPRGVLLYGPPGTGKTMLVKAVANSSTASFIRVNGSEFVQKYLGEGPRMVRDVFRLARENSPSIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQTSSVKVIMATNRADTLDPALLRPGRLDRKIEFPSLRDRRERRLIFSTIASNMSLSPEVDLDSLIIRNDPLSGAIIAAIMQEAGLRAVRKNRYVILQSDLEEAYTAQVKTDSEVDKFEFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4F8</accession>
    <name>A0A1E3P4F8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Endosomal/vacuolar adapter protein YPT35</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">PX domain-containing protein YPT35</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Sorting nexin MVP1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_13664</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Recruits the lipid transfer protein VPS13 to endosomal and vacuolar membranes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the YPT35 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039405.1">
        <property type="nucleotide sequence ID" value="XM_019180580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4F8"/>
    <dbReference type="STRING" id="683960.A0A1E3P4F8"/>
    <dbReference type="GeneID" id="30197826"/>
    <dbReference type="OrthoDB" id="10254720at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07280">
        <property type="entry name" value="PX_YPT35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028662">
        <property type="entry name" value="SNX8/Mvp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037917">
        <property type="entry name" value="Ypt35_PX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46571">
        <property type="entry name" value="SORTING NEXIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46571:SF1">
        <property type="entry name" value="SORTING NEXIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="PX" evidence="12">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ60198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="110" mass="12459" checksum="0F3E9DEC1E196247" modified="2017-01-18" version="1" fragment="single">WASDVRIGDCTIVTGSGAGQKFAVWSINIETSKGGHIHLLKRYSEFDELRNKLVQSYPDHITEIPKLPPKKAFGNLKNDFLVKRRKGLEFFISCVLLNPVLSNSDIVKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4I0</accession>
    <name>A0A1E3P4I0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_105119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
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            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
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        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
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    <sequence length="161" mass="18746" checksum="39C1FE39FE6855CC" modified="2017-01-18" version="1">MSGKSVKNGKPKLRKGAVGGLDIRDLIRKAQDEKHEKEDAKVEVDNEPNESDRIIAEEEEEEQKVSISKSLLKDSPVGELDQEAEDSDGVPLPRKKTEYEKYLKSKERDKQIRKYNEECIKEYREERAQMRARVRQRDHSLDEQETVSIEASPSKKVRFKF</sequence>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
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                <person name="Lopes M.R."/>
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                <person name="Salamov A.A."/>
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    </evidence>
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    </evidence>
    <sequence length="473" mass="52396" checksum="1C4F8AE8C836477F" modified="2017-01-18" version="1">MALSSIETALTKHIRSLLQTHFSTKSLKRLACSISLISCLCAGSVLLFALFTPVLQHQLHYTQFQINIIGSFTSIGMYLPLPVLGYLADCHGPVLLSVISVLFFSPGYTLAATVVQKDWSFWYLAISFGLIGCATSALYFTALLTCAKIYPKSKGLTISAPVTCYGLSSLIGSRVLKLSCLQKNGDLDLYRCFKFFSFLYLFLGLFNWVSASVVSIERDVLLRKHEDGENTPLLTDPNQEHENNDDLVPNHKSKFLKFIKDISTYVLLFSLLLSIGPSEVYITNMGSLVKAITPNSLISDQVAIHAVFSTLSRLSLGALSDFLVTNYQISRSWLLLSIIVLGFFTQIFIATSTFVKDQYYIISALSGFSYGGLFTLYPTVIFSIWGPEIFGSAWGSFMIAPAIGSTTFGMIFGLVYDSACGVFTESTTGNCVSLCSTITHCMERNLDQKRSLIIIDHLHYINYIYLLILLFNS</sequence>
</entry>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
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                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
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            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
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        <location>
            <begin position="761"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="804"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="815"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="845"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="863"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="877" mass="85399" checksum="04B858FC29951B5F" modified="2017-01-18" version="1">MKETKSGETSGATANQGANSGYGSSSSNANTSNVGSSSGYDTGTGAAAGAGIAGAGIAGHHASKSGDATATSGSSSHPNSSGNTTGYNDPSTTSSRQPQVSAGSSSGYKDQTSSRGATQPDYETSSSSAAPVSRETQPSSSSSHGYGKEATGAAAGLGAGGVAGGGVASNSSGSTEQSGTSENRKSGLFSRFSKKDASSVAKGAKEDASKFSGQAQGIASDVKKGDYSSAKKFAADAKENPDKYVGNTRSAFNESKSGSSAAGTKDTTNAASQVPSDSGHTSGHGVGKGAAVGAGAAGAGAYAASNSHSGSTQQHQSSGKSTGASGSGSSSTGAAGATLPPQLASDGSNGYQSKSTSGAHTSSGHESSTGYTGSKHDVFGNQPPQALKNSDAKGYTSAQQAEQEPSSGGQSTGGQSTSGHAGTGAAAGAAIGGAGVAAHSQGKSNEAPSSTKNEQGIPQNLSNPFTDGSAPQQQPNNPNADDVSGGEQKTLDPKSADQVKTSSGPDHQAPKSSTSKPSKVAATEDSHPTSLGVDNQDKSSGSGYGFGGIAAGLAGAAGATGLASKIGGGGSNQETKEGSIPSGGQKVSSNLSKDAAGEGYLETAKGYAENAGSTFNDYVQVAADKADEYAKGSDNKYIGQGNEYVQQLAEKSDEYAKQHGESSKNKSVSDSKSSAGAAATKPSSGGKQSGQELSNKSSKVGGAGASSGAAGATGATGAATSGSTSGAAGSTSGTSASGSSAAAQFANKKSGQDEQEQSESLPKHFQHENKNPFSGKTGPVPGTEDQVIPHTQTSDIPKAEQGYGATHAAHGHTASAQPTKASDSLQPPRANAGYDTDSNSSTSSPKKRGLLSKIKQKVKGESSAPSSTQGTPKKTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P574</accession>
    <name>A0A1E3P574_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromatin modification-related protein EAF1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Chromatin modification-related protein eaf1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 15">ESA1-associated factor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Vacuolar import and degradation protein 21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_29944</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039675.1">
        <property type="nucleotide sequence ID" value="XM_019181669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P574"/>
    <dbReference type="STRING" id="683960.A0A1E3P574"/>
    <dbReference type="GeneID" id="30198915"/>
    <dbReference type="OrthoDB" id="5364245at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000484">
        <property type="entry name" value="Chromatin modification-related protein EAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46459:SF1">
        <property type="entry name" value="E1A-BINDING PROTEIN P400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46459">
        <property type="entry name" value="E1A-BINDING PROTEIN P400-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00573">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSA" evidence="18">
        <location>
            <begin position="245"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="17">
        <location>
            <begin position="408"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="529"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="577"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="626"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="21"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="111"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="626"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ60468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="703" mass="79777" checksum="9501ECDACA528205" modified="2017-01-18" version="1" fragment="single">MSFLEKNDLQNGHIFDSKTLQSNNASTQQTPSQSQNQPQEPQSASNPSPQQPNQQDAETESQLQRSSQQSQAQPITNKPPPKEAATSDNEVRVQPNITDSTSDANEPSEVPVEKVESKETRIDEEQNKQPEQEIQKLPRRPIVKKRKTSDNKISLFDPFNKEAIDTHVLLLKEQLPPRIAEATPLAELYYLAQTLPLVKLMPSTHKVITSTMFETALTEGKINVVHSRIEELKRQGKWSLRQPTKFIDPIRGNTKTHWNHLLSEMNWMATDFREERKLKIATCAYIAQSISDYWNYGKVSFKASIDFDSLTDQNRTLLEEMPAFTSFEQYDDKFSSAPLVPVSKSLIPIEDDTWYNVLFKQIVDESDSVPAQQKGLFGFSSQKRINTSIKPPPPPSLKYLDLRTPTIWLPEDDQNLIEYVNQFSFNWGVVSAYLSKKPTRSYSSNIERRTPWQCFERYIQLNDTFQINDMRGQNTLSAMKWLEHAHNIQATTKRRISPLGVGVDSIQRGHKRLRWASMFEAIRKCMRKRESVTRPNNNQVRKNGDDKKLGAPTPAELSKLKFERDKAIQEAYMQNPVNGFRGRMPQAAPAPSSTVAQPNAMPKITGPNGVPYTPEQVQQLMQLRQRKLMQQAQQQAQAGTNGSQQVNPTPNGTNNNGGNGNNGRKLTFVPAHVSAIINQIQSQNPNMSKQEVTKIAAQYLANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5P3</accession>
    <name>A0A1E3P5P3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS15 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_28902</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039771.1">
        <property type="nucleotide sequence ID" value="XM_019181550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5P3"/>
    <dbReference type="STRING" id="683960.A0A1E3P5P3"/>
    <dbReference type="GeneID" id="30198796"/>
    <dbReference type="OrthoDB" id="623277at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.10:FF:000003">
        <property type="entry name" value="40S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.130:FF:000001">
        <property type="entry name" value="40S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_A">
        <property type="entry name" value="Ribosomal_uS15_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023029">
        <property type="entry name" value="Ribosomal_uS15_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012606">
        <property type="entry name" value="Ribosomal_uS15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006331">
        <property type="entry name" value="PRK08561.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885">
        <property type="entry name" value="RIBOSOMAL PROTEIN S15P/S13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885:SF6">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08069">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01386">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS15 N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="151" mass="17006" checksum="5E0B50F4135D4C39" modified="2017-01-18" version="1">MGRLHSKGKGISSSALPYSRNAPAWFKLSSDAVVEQIIKYARKGLTPSQIGVLLRDAHGVTQARVVTGNKILRILKSNGLAPEIPEDLYFLIKKAVSVRKHLERNRKDKDSKFRLILIESRIHRLARYYRTVAVLPPNWKYESATASALVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5P4</accession>
    <name>A0A1E3P5P4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_31459</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039364.1">
        <property type="nucleotide sequence ID" value="XM_019181834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5P4"/>
    <dbReference type="STRING" id="683960.A0A1E3P5P4"/>
    <dbReference type="GeneID" id="30199080"/>
    <dbReference type="OrthoDB" id="1713558at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050756">
        <property type="entry name" value="CSN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013586">
        <property type="entry name" value="PSMD3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057985">
        <property type="entry name" value="TPR_PSMD3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758:SF2">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3/COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08375">
        <property type="entry name" value="Rpn3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25573">
        <property type="entry name" value="TPR_PSMD3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="225"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="472" mass="54727" checksum="B641ED37062566AE" modified="2017-01-18" version="1">MTQEEQIVVDNEPTPEELEKLTISKIEAAFQLVSKTVKTFDQRYVFKTLRDLVSVRKQLNADVLSYLINTYLNNPYREYLLEFIGGKITNSEEKGEILPEINFLIHLLVQFYLLDSNQLAKLDELNTQYILKLIKNYNRRSLDLINAKIWFYIARTKELINDLISIRPELLLALRTSTIKHDDETSGSLITLILRNYILVHDYSQASNFIEKIELPKNVSNSIETRYYFYLAKINSIQLNYSDSHEFVITAIRKAPQTQNAIGFLQAAYKLNVLIELLTGDIPELSFFQKPGFEKVLKPYQELTKAVKLGDLKIFNETLINYRDSLIKDDNYSLVTRLRSNVIKTGIRIISLSYTRISLKDICIKLHLDNESSAEYIVSKAIRDGVIDAQINHEKGYVESNEIKNVYSTNQPQDQFNQRINFVNSLHNDSVKAMRYPPSVNRTENSGRSKEIEDDEAELINALQEDFDEDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6D3</accession>
    <name>A0A1E3P6D3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_29552</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040186.1">
        <property type="nucleotide sequence ID" value="XM_019181625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6D3"/>
    <dbReference type="STRING" id="683960.A0A1E3P6D3"/>
    <dbReference type="GeneID" id="30198871"/>
    <dbReference type="OrthoDB" id="2399539at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47431:SF1">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47431">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="27"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="670"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="720"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="670"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="779" mass="88087" checksum="C7A9CCF1DCCC44FF" modified="2017-01-18" version="1">MSEEQRLPESLNDSQKARPNAKHVSRACLECRHRHLRCDGQYPKCGRCTQHDKECSYVKSNRGGSRKKGVKKAKPSKTDITDVDIEVPILPCIPNNGGPVDHNEANCPNGHDKESCSFLRDGSQLNLPCTKKQKANTIQERGEQNVNMEPFHKQPSAAGRKFLDQVFDNSSIINNYYTRFHVAHPLLPSIDEIDNFLLLSDEPRELLAVMKLIGDGYTSSKYSKNINQVFDIVIEINGLIEKKPRDLITLQTLILLSLVCHISALHDLSTSIRKKCMELAIHLDINNLDIERFKGDIDINGNSLNKKGASSSAVNGLTPTGSVDELNVNFYNTKRVRNLEEETLKDCARRCFWELFFLDIIIGCSDGKTVSSLTSLECFVNFPNKPDRFEFDYETRAHTSKLVDDSVRLNNIIFSNVSIGHQYTKLSASLSNWELKFSNPDFYKLPYLVDGMGRINNGVHQGILMLNYAKIFTHRPLSFLWRPDIPKNLKCVEFLNDQLNKTDDNCQDLPSDKDLIKSRKIIETRKTIDAANSISKTLIDTNPLDILKRTPLSACSLAFAGLVHLSSYLWYSQVIQSKDPNLGSFNSNELNIYEEYLKLELSGIYQISSHWYLSSKIANYLLESITKLLPDLADKLHSHFHNDANYANVRAQKEDENEFDASRSINSTNIDNLSISNNSESTPVNHVSTPLNGSSTPGTDFNRGFPNDPTYSSLNLNTVQQSDLPWPNSEEPMSPQSDTGCNWIDKNQLDFEFNDYNFAEFDLNTINAIDDMIKNATTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6G8</accession>
    <name>A0A1E3P6G8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_84312</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039664.1">
        <property type="nucleotide sequence ID" value="XM_019185687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6G8"/>
    <dbReference type="STRING" id="683960.A0A1E3P6G8"/>
    <dbReference type="GeneID" id="30202933"/>
    <dbReference type="OrthoDB" id="5069333at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF12">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="6">
        <location>
            <begin position="291"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="819" mass="94105" checksum="DBFA3B9A153FA0B6" modified="2017-01-18" version="1">MNNSVLSQSDFGEHNNDVIDLYESYNALSMKNSSHEEHTPLNPMAYHKKDHYTAFFVCYMFLTIRATTSSICEKFKSTWKSNHSDGASKWLLLLDLKYQDSELQESIQRIINDRAKLYEANTSPFLRLADSNPENVEGLIKTIEQVLPSKVLCENYLDFFFHFMSILRPFVDEDTFKKDVARIISFDDVTLKPKVKFSERRDCAIVATFLIIIRYASIAVSVIDEEELPTFLIPLKENPVSVKAIPVAQMCLSIYKVLRKSTIHILQALLYLRMYFKDCPEDGDGLTLIQSQQLFGFIVQSALAMGLHRDPVNYSQISNDVKESNLRRRIWYSIVNIDTETSILAGSLSVLPDPSLVNVKPPMKTSVDSIENAQAEDLEKSTELNQIWQKFCNEVNNMQEKPTLSTLVGTLERARKFVETNYSMKDMISCKEMNCGSRIFRSSMFKNVKILEKNLLQLSLELSFYVGLTIHYESNAHLNADLYKIFFKKSLENLVSALDIAGAYLAGAFNDYFRSVYTNFTLLPLLNTTIYRASNIVVGSLLRAYHAQELLNSKFYSSKSNVSSEEFEKLINPLCKRYEDFMFIYKNRLGTKYYQSLKMTGAGKYSFVALKKNKFTIMNKIIKYLESNEKGQINEDLFLNGSKTREEIKKTFQSNKSLIRVYAEWMQLSNSSKWENGNRITKEKDDDKPSEEPATIININNSNMLLEFTKEEIHEYSDILINAFRFCSFPKNTNMDASKELLQEHTTSSTPSELYNEEFYDKIFHDSDFDSQMSRLFKASKGIAPGNDYTASDGVFGEIFDQLMGSSTATVDDILKFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6P1</accession>
    <name>A0A1E3P6P1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar-sorting protein SNF8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_51793</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040167.1">
        <property type="nucleotide sequence ID" value="XM_019182678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6P1"/>
    <dbReference type="STRING" id="683960.A0A1E3P6P1"/>
    <dbReference type="GeneID" id="30199924"/>
    <dbReference type="OrthoDB" id="283883at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016689">
        <property type="entry name" value="ESCRT-2_cplx_Snf8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040608">
        <property type="entry name" value="Snf8/Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12806">
        <property type="entry name" value="EAP30 SUBUNIT OF ELL COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12806:SF0">
        <property type="entry name" value="VACUOLAR-SORTING PROTEIN SNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04157">
        <property type="entry name" value="EAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="26481" checksum="EEE84FAB296F83FC" modified="2017-01-18" version="1">MKKVGLAAFDDNKLRQYSELGQSLLQKKTNELTTQLQLFKSILLNFLKEHNEELSKDITLRNEFIKICQSVGIDPLQIYDKHDSKMNEFYFELCVRIIEFSNSMKDMNGGLLPVNEFLSSFKDLNITFKDVEISIGLLSALNDELKIIKIGSKHYIKNFNIELTNDQNMILETVGNIGFISVAILRDNFGWKSYRSQSILNEMISSGYLWLDDNNGELLYWDPAWITKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6V5</accession>
    <name>A0A1E3P6V5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_40880</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040368.1">
        <property type="nucleotide sequence ID" value="XM_019182559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6V5"/>
    <dbReference type="GeneID" id="30199805"/>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="114"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="12">
        <location>
            <begin position="29"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="252"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ61161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="347" mass="40624" checksum="D420AEEDF639CA45" modified="2017-01-18" version="1">MIWVSYIIAIYKEPGSPPKNFEPAPGEWKRWCKKCQLYKPERAHHCKTCKKCVLKMDHHCPWTANCVGHDNMPHFLRFLLWVDYTTAYTLYYLFKRLLSYWNDRNLPAYLISKVHLTATIVLIPLDFFVLLTVGILTFRCFYHIIVTGMSQIESWEYERLESQLSSERLRNKIKHNYELLFGKELKDVTSWSSGANVLREPPETNIPFFTVDDLVFPYDLNPWSNLLQAFGYPWNWILPWGKPLGDGLSYPKNEYANPEDQDLSSLPWPPDGGHQDRINGEHLFNDTIQDNEQVVQRRMVHDPRNNMNRTEWYNDFGEKLSDFGVDVEAETFDTPSAAVGSSSEARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P714</accession>
    <name>A0A1E3P714_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATPase expression protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_77861</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040426.1">
        <property type="nucleotide sequence ID" value="XM_019184624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P714"/>
    <dbReference type="STRING" id="683960.A0A1E3P714"/>
    <dbReference type="GeneID" id="30201870"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051240">
        <property type="entry name" value="Mito_RNA-Proc/Resp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47933">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47933:SF11">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="618" mass="72204" checksum="69CFD04A2FCA218B" modified="2017-01-18" version="1">MSLLKTFRDYVRICKDKNLLIHPRGQNTNFLPIALVNEVSHQLQLSEPKPKQNDAQLVNDYHEKIGKGSMFERKSVESYLKPLGSTYLKRANIEADEFFQKQEAQERHIFNKNLKSLENSSTVMFNLEQENIRLILEKLVAVTPAPPDPTTAAPKISLNDESKRYKKKPPSHVYTEIPPVPDFYDVSVFEEYIYLLTHSTFHHKKSSKFNGIIPKILKNLFHPLNSNTISLRTVNSYNDVIFYFSKKWNIATCRELLVQMKIENVKPNATTFNLMLKNLLTHQTIRHVADPYKISLQYLTEMKKYGIEADLVTWNIMFNLLKDDISKKLLIENRKLLCLPADPFFLKAVFTHISHQTGTTSSELLKMVQEFDVSVDVSLLNVVIKKLIQEQQIYSAWKVIDHSKKEFSFNPNVDQMNLLLVPFSNTGRVDLVVATMNTFKKRYKVKPNYDTYNLVMKSVSRARHWDGKLSVLRILYHKMMLDLKQNVAGEYWIRRTRARLKFIYQKDVELKPDLSTEESQLKLDMDLLRWGLDSDVIDLSKKVPDQFEEITAKLGFSKEQRDALFIDDLDRKKIKMKKYKHRIGVLSIQKSLVDRIPYAEDSYAALKKEMNKRRLIET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P745</accession>
    <name>A0A1E3P745_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_29044</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PER33/POM33 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040452.1">
        <property type="nucleotide sequence ID" value="XM_019181568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P745"/>
    <dbReference type="STRING" id="683960.A0A1E3P745"/>
    <dbReference type="GeneID" id="30198814"/>
    <dbReference type="OrthoDB" id="5581259at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051645">
        <property type="entry name" value="PER33/POM33_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005344">
        <property type="entry name" value="TMEM33/Pom33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03661">
        <property type="entry name" value="TMEM33_Pom33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="246" mass="28653" checksum="95D44F8EA8934466" modified="2017-01-18" version="1" fragment="single">TPQFFWFLGHVSTILNTLSTVFFSFFSQSSYKRYYNWALISIISTYLVVLFQIFKSSKTINFFNFKTFIYNDNFQYLSLAITWYLVSNVKIISGGLYPFFIFSSFHALNYFKNFILPILPSVSSPVKDHYSKIINTLITTYNEQSLVAASNLEIFILVQLSLSSPLIIFQIFRNFKLAFVNIFVLLQYVTFIKLRYKQSKHTKLLFDGTLYKVEAVINSGRIPPVLANYYHQLKSKATHFIGLIPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P752</accession>
    <name>A0A1E3P752_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_90299</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040261.1">
        <property type="nucleotide sequence ID" value="XM_019186226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P752"/>
    <dbReference type="STRING" id="683960.A0A1E3P752"/>
    <dbReference type="GeneID" id="30203472"/>
    <dbReference type="OrthoDB" id="1708823at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="279" mass="32796" checksum="C05DC72A68ED8545" modified="2017-01-18" version="1" fragment="single">MIDLRILSPWDELIDYSNSIDLHNLDIEKHSQIPYLIIQLKALQDWSSKHNGEIPNTSVEKKEFKDLLRTWKKDYNELNFDEAIDNSHKIFNKTKYQSNVQEIFDKTDEYFNDDHKRSHFWILVKALKEFSIQNDGFLPLSGELPDMDSTTENYITLQNIYKAKAQKDLESFTKILLKVQESLPLPVTIPKETISSFVKHSKFLFFAQNSKNLITNNFNQIVGEETTSSQILLSFLVYEKYYLTKGKYPKLQDLDELINLTHDFIATDNDKLHNILHEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P788</accession>
    <name>A0A1E3P788_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol-4-phosphate 5-kinase</fullName>
            <ecNumber evidence="2">2.7.1.68</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">1-phosphatidylinositol 4-phosphate kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Diphosphoinositide kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">PIP5K</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_89930</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14425"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.68"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.1.68" evidence="2"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039944.1">
        <property type="nucleotide sequence ID" value="XM_019186200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P788"/>
    <dbReference type="STRING" id="683960.A0A1E3P788"/>
    <dbReference type="GeneID" id="30203446"/>
    <dbReference type="OrthoDB" id="20783at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031321">
        <property type="term" value="P:ascospore-type prospore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17303">
        <property type="entry name" value="PIPKc_PIP5K_yeast_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000009">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase its3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF8">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 5-PHOSPHATE 4-KINASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="13">
        <location>
            <begin position="259"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="193"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00078403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00080374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00082306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ60737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="654" mass="75007" checksum="FF0439190C9E2416" modified="2017-01-18" version="1">MLEPQIEQAQQLPQLKEKKVQEEITIPTSTSLELHKNTASSPSTPLSRVVTTTDDSIIFTNPSSGESENTTTSHSNNIPNGQNLAIDSTETQTQLNILGNDNGSDGELENFYPASENPQQIQNNYEADEINANESSKLLPPQDQNPSVNSFHDQLNSFSFGNRPASFHENGSYIQDDDDEQVLLNTNVRRGRGSRATGVEPIDPSSIRNRNSTFTANSKNQNADSATMEIERMRNSILLKRENKKRQKTVLDDDKVLVGNKVGEGHVNYVIAYNMLTGIRVAVSRCSGIMQPITDQDFRSSKKLVFDVSGNELTPSSKYDFKFKDYCPTVFRELRMFFGLDPADYLVSLTSKYILSELNSPGKSGSFFYFSRDYRFIIKTIHPAEHRHLRRILKDYYNHVKNNPNTLISQFYGLHRVKMPINYENLKRRKIYFIVMNNLFPPHRDIHRTYDLKGSTLGRFTPNDYPAHRVLKDLNWIQNQETINFGPVRHKLFIDQLNRDVKLLIKLNIMDYSFLIGFHDLKKGNKEEILQQKKLSIFSPASSNLEDLRKTNPKLLNRYNDLPSIDFKNGNIFYSNNGGVGSTDANDEEGEFIYYLGIIDCLTNYSIIKRLETFWRSLTNDRKIISAVPPREYGERFLKFITHSVNENTLEKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7D6</accession>
    <name>A0A1E3P7D6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">10 kDa heat shock protein, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_77939</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GroES chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040510.1">
        <property type="nucleotide sequence ID" value="XM_019184636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7D6"/>
    <dbReference type="SMR" id="A0A1E3P7D6"/>
    <dbReference type="STRING" id="683960.A0A1E3P7D6"/>
    <dbReference type="GeneID" id="30201882"/>
    <dbReference type="OrthoDB" id="184876at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045041">
        <property type="term" value="P:protein import into mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.33.40:FF:000002">
        <property type="entry name" value="10 kDa chaperonin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="entry name" value="GroES chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00580">
        <property type="entry name" value="CH10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018369">
        <property type="entry name" value="Chaprnonin_Cpn10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772:SF0">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00681">
        <property type="entry name" value="CHAPERONINS_CPN10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00056825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="11306" checksum="68A667BD76235804" modified="2017-01-18" version="1">MSTLLKSAKSIVPLLDRVLVQRVKAEAKTASGILLPEKNVQKLNQAKVLAVGPGFTDQNGNKVVPNVAEGDTVLIPPFGGSTVQVGDDEVILFRDSEILAKIREEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7K3</accession>
    <name>A0A1E3P7K3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Required for respiratory growth protein 7, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_61954</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRG7 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040603.1">
        <property type="nucleotide sequence ID" value="XM_019183284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7K3"/>
    <dbReference type="GeneID" id="30200530"/>
    <dbReference type="OrthoDB" id="20734at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018828">
        <property type="entry name" value="RRG7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28133">
        <property type="entry name" value="REQUIRED FOR RESPIRATORY GROWTH PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28133:SF1">
        <property type="entry name" value="REQUIRED FOR RESPIRATORY GROWTH PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10356">
        <property type="entry name" value="RRG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="167" mass="19249" checksum="86186209E70E75FC" modified="2017-01-18" version="1">MLLRPFKNKVIPTLIFKRYRSAKPSYNDLESYLRYAEFVKLRKESTTFLGTTFEMKVKEKLQSALNIPDLQYSGGAYDNGIDLIGKLDISQFKTEQAPEKPTYVVNGKRIKPLALRKNMDIDVLVQCKSFAFKISAKEIRELSGIFHYNIAPKDRNKTLVIMTAPNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7M0</accession>
    <name>A0A1E3P7M0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Arginine N-methyltransferase 2</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_28386</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">S-adenosyl-L-methionine-dependent protein-arginine N-methyltransferase that methylates the delta-nitrogen atom of arginine residues to form N5-methylarginine (type IV) in target proteins. Monomethylates ribosomal protein L12.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. RMT2 methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040497.1">
        <property type="nucleotide sequence ID" value="XM_019181481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7M0"/>
    <dbReference type="STRING" id="683960.A0A1E3P7M0"/>
    <dbReference type="GeneID" id="30198727"/>
    <dbReference type="OrthoDB" id="19014at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019702">
        <property type="term" value="F:protein arginine N5-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000310">
        <property type="entry name" value="Arginine N-methyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017408">
        <property type="entry name" value="Arginine_N-MeTrfase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051038">
        <property type="entry name" value="RMT2/GAMT_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026480">
        <property type="entry name" value="RMT2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379:SF1">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038148">
        <property type="entry name" value="Arginine_N-mtfrase-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51559">
        <property type="entry name" value="SAM_RMT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RMT2" evidence="8">
        <location>
            <begin position="192"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="141"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="141"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR038148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="429" mass="49661" checksum="08C6EAF0CDAF34A3" modified="2017-01-18" version="1">MSELHDLCRLTKRPIDQEYIKNLQYYLSNGIPATYTLEEAENFELGKEDGELTSTTTPLHLISYNIPNDLTEGETQIVLQIIDELFSWGAGWNLTDKNGDTPGDVLFKKSLRGSDFYSRFVDAGVRAEILLRKINDDIEFLSDAEEPEDTNDEEVPQLQAEEEEQEEAQPTEEEIKKQQEFERIKNELINDPANTQSTYLKTKLEYKDGALITKDNLDGVMMDWENELMKAGCDTIFKSLEYAEDEDDIKEVNILNIGFGMGIIDTMIQSRNPTHHYISEAHPDVLAKLRQDGWFEKPNVTVLEGRWQDTLPELLSKGVFFNGIYYDTYSEHYSDMLELYDLIVGLLKPSGVFSFFNGLGADRQVIYDVYKKIVEIDLNNYGMDVKYTELETPKSTGEEFDDTKESEWQGIKRAYWRCKTYYHPEIRFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P805</accession>
    <name>A0A1E3P805_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_78113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040695.1">
        <property type="nucleotide sequence ID" value="XM_019184665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P805"/>
    <dbReference type="GeneID" id="30201911"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133727" evidence="2">
        <location>
            <begin position="22"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="178" mass="18802" checksum="D266D9515A232604" modified="2017-01-18" version="1">MVSINVVLQTALFALTVQSAAIPPSLNSSSLPTPGTPGTPLTFPKRNFNETLSKRGFNETLSKRSFNETSVEKRLFNETNGVKRDLGASLSDLPKPSQLAVSDFPSVSIPSIIPSSIPSSFPVKRGFNDTEGTGIYKRGEELESLSEPPALNGTTNGTNSTGPISYGAPSSAPLPSNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P834</accession>
    <name>A0A1E3P834_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Photolyase/cryptochrome alpha/beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_26253</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="5">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA photolyase class-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040745.1">
        <property type="nucleotide sequence ID" value="XM_019181239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P834"/>
    <dbReference type="STRING" id="683960.A0A1E3P834"/>
    <dbReference type="GeneID" id="30198485"/>
    <dbReference type="OrthoDB" id="435881at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003904">
        <property type="term" value="F:deoxyribodipyrimidine photo-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043153">
        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.579.10">
        <property type="entry name" value="DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005101">
        <property type="entry name" value="Cryptochr/Photolyase_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002081">
        <property type="entry name" value="Cryptochrome/DNA_photolyase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018394">
        <property type="entry name" value="DNA_photolyase_1_CS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455">
        <property type="entry name" value="CRYPTOCHROME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455:SF18">
        <property type="entry name" value="SI:CH1073-390K14.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03441">
        <property type="entry name" value="FAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00147">
        <property type="entry name" value="DNAPHOTLYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="Cryptochrome/photolyase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00691">
        <property type="entry name" value="DNA_PHOTOLYASES_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3 5" id="KW-0274">FAD</keyword>
    <keyword evidence="2 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="7">
        <location>
            <begin position="78"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="338"/>
            <end position="342"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="380"/>
            <end position="387"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="478"/>
            <end position="480"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="6">
        <location>
            <position position="412"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="6">
        <location>
            <position position="465"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="6">
        <location>
            <position position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="576" mass="67280" checksum="F17A18A3DF365C9D" modified="2017-01-18" version="1">MPLKRSPSPNSSASSSATKKFKTSHHLDFSHYINLNSKSYPFKHSTKAAIKWNNGSKKKPFEIFEDKYNEGKLNEDEKYLVHWFKNDLRIHDNTSLYHLVQDAQKINAKPIGIYIISLKFLESHLESALKVQFIQNTLKVLRESLSKLNIPLSIIYLDDSKDKYKLSSNSYDSFKDWLTALLNEKFQTNSIYYNVNYEYDELNRDISLLSSNLNIKAFHDQCIVKPMELTSGKGTQYSKFSPWFKKWDSYLNSSPIELLQIQHKFEKWDWSHLPNDDDYKLPPQFQVSSLKEVGVTDIGNFENLKGGEDEALEKLEKWISNSIKEYQSEKDYPSLSSTSRLSPYITQGSISPRLIVTKCQEANNNKRIGGNTGIETFIKEVAWRDFYRHVTVNWPYLMMFVPFNLGLIDLKWSDDYSRFEKWCLGETGFPIVDASMKCLLHTGYMNNRCRMVVSSFLSKDLAMDWRMGELWFYNHLIDADLSSNNGGWGFSSSTGLDAQPWFRIFNVYTQSEKFDPDGVFIKKWLPNLSKLENFHEPYNEKIDAKEIEKLTNGYPKPIIDRKQAREETLERYREAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8A8</accession>
    <name>A0A1E3P8A8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_75849</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040850.1">
        <property type="nucleotide sequence ID" value="XM_019184271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8A8"/>
    <dbReference type="STRING" id="683960.A0A1E3P8A8"/>
    <dbReference type="GeneID" id="30201517"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034593">
        <property type="term" value="F:phosphatidylinositol bisphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043812">
        <property type="term" value="F:phosphatidylinositol-4-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022158">
        <property type="entry name" value="Inositol_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662">
        <property type="entry name" value="PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662:SF7">
        <property type="entry name" value="SACI DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G15890)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12456">
        <property type="entry name" value="hSac2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="2">
        <location>
            <begin position="264"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="867" mass="99460" checksum="D1AD284937993BCD" modified="2017-01-18" version="1">MADNPSIVSSDQASTQTGYAHYLELGLYKSGLKLSLQNSDRSHFIHFEDLSVTPDYAPQGQQIASLNSLGLVGFVDLGPGYIIVIHTQAPVFEHKDVTIWNILKSLVIPLKFDLAKKFFKIEDGVLDEEDEASTEPDENQNELQQDPQSPNTVGEKINQTKDIFFSRLGVYVNDLKSNPSLSISNKIVNLLETENESDEIDENDLNSIKLKRSITIRGIPKDSTPKKQNKLTKFVDKIKQQQLDQKEQRYNISNSMLYKFEKQLQYFFSSRDFFYSREINLTRDIKFEKGELVTFDDSKHNSFHFNHSITQKFKDTDLSLSLIQGFVGSIDIDLNLNGSLSSRQGKLLLVSKRSTKRAGSRYLRRGIDDHSNVANFVSTSQILLTELNSGPLCAKFDIIRGSIPLFFQQNPAFLKPIPTLTRSIEKCEKPFKEHFENLLEKYGSVSCISLVERSNREKEIGEAYENLAKKDNIEFTWFDFHEVCKKMKFDNVSKLLNYPIDNNESDLTIDEQLENYKWCDNITGDDQLGILRVNCIDCLDRTNIVSKYVSETMLLQQILPQHDLTIDSLPQFQKLYNNLWADNGDFISRQYASTNALKGDYTRTAKRNYKGLLNDAFLTMSRYYSGYISDYFKQCVVDYLLGHKNQEIFEEFESTLNVIDPNEINENLNKKNSSLKFIIEELGILKSKGDDDGCCEDEEDLILVGSWSGLQSPMDFNTLKLGQTLNDISLIVTNKVIFFVKFNESESKIEKTIKLRVHDIKELNYGVFITSTHSSLSLNPKRNIGLKFVFTSDVNTSDEIEPTNDYLVVKFPMDMDYDKAKYIIELISKTCHSSTLSNVDLMNEEDAKKNTNIIQNLEYRFKKLVWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8I8</accession>
    <name>A0A1E3P8I8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Actin-related protein 2/3 complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_26358</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">Cytoskeleton</location>
            <location evidence="7">Actin patch</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the WD repeat ARPC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040932.1">
        <property type="nucleotide sequence ID" value="XM_019181250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8I8"/>
    <dbReference type="STRING" id="683960.A0A1E3P8I8"/>
    <dbReference type="GeneID" id="30198496"/>
    <dbReference type="OrthoDB" id="406844at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000601">
        <property type="term" value="P:positive regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017383">
        <property type="entry name" value="ARPC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10709">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2/3 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10709:SF2">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038093">
        <property type="entry name" value="ARP2/3_su1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR038093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="365" mass="40159" checksum="D46C07495180750C" modified="2017-01-18" version="1">MSSQPKVIQFHHQPIQSHCFNQDRTILAITNETNVEIYDLKQSPPKLITILKDHDKLVTAVDISIHGRIVTCSQDRNAIVWEPLSDGSYKPTLVLLRINRAATTVAWSPNGFKFAVGSSARIISVCYFEQENDWWISKHIKKPLRSTILSVSWHPNNVLLAAGSTDGHARVFSGYIKGIDTKPEPSYWGEKLPFQTLCGDFISSNGGWIHDVKFSPSGDVLGFVAHDSSLTIVYPGGVDNPPKSILSVSTSFLPFKSLVFKNENEIIAAGHNCHLVVFQGDESNWVQSGAIDDPNKNDDKTVGETSALNMFKQLDLKGTLSGNGSGLKTIHQNTITELRPYQGSSNELTKVSSSGIDGRVVIFNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8L6</accession>
    <name>A0A1E3P8L6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_102545</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040857.1">
        <property type="nucleotide sequence ID" value="XM_019180358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8L6"/>
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="ODQ61650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="424" mass="46305" checksum="BD33ACEBFFDB8DDF" modified="2017-01-18" version="1">MQIFRNVLAFIALSSTGFNSFGAFASPVPESELIADEPQPADQINEPVSLEKRTILGDAMLSQGGVVVTYGSSAATLLLAIVCPGVTQVYAACAGLASIGLAASSFGMALNTAGFFLEIGDALTPALGHVLGKEEDHVVTDQVFDHYKEIWGDEIDFNQFYNVSGDDFESQSLDVNDIGKRAFSKVELLKEITGIDTLYNKVYDISDQSVDAEDIPESPFIEKVWEVSPRLRQGDGYRDRFIRFFQSFKANPTILIYKSIYGVHVSTPISPLTNKRRLIKSIFNATPSSGQAFESFGLVSNATGKFTKGIKKVKDSYLDGFITLTLDVSKKSADFWGPMLNSYFEKEHSAYTMENFAINGTGALLTTEEAFKTDDGSVKVCGALFKGEVSPEPSRLHTLKHLLRTQAYFGTYGPIDPGCHSLFG</sequence>
</entry>
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    <accession>A0A1E3P8T8</accession>
    <name>A0A1E3P8T8_WICAA</name>
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        <recommendedName>
            <fullName evidence="4">Vacuolar membrane protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">WICANDRAFT_14853</name>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ61825.1"/>
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    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="STIMATE/YPL162C"/>
    </dbReference>
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        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YPL162C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31735:SF1">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YPL162C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12400">
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
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        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
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        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="24739" checksum="9D5FCD660012B870" modified="2017-01-18" version="1" fragment="single">DQCELLGPVSILIQSLLGLIAISSLMVKRLYEHPKRPWKVWFYDVSKQVFGALGIHLLNLTVSLLKSKKALLMLTQQDGGGDEEDHNQCDWYFLNLLMDTTIGVPVLWLCLFAVYQVCEWLQISEIESGNYGKPPKFSSYLKQLTIFFIAVLMMKGILYLLLYIPIFIMYADWILSFSDDFPNLQIVLVMLVFPTILNCFQYYVIDSIIKFPSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P921</accession>
    <name>A0A1E3P921_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_15090</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019180630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P921"/>
    <dbReference type="STRING" id="683960.A0A1E3P921"/>
    <dbReference type="GeneID" id="30197876"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF78">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="373"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="492"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="21"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="526" mass="58590" checksum="3F32B86F7585098B" modified="2017-01-18" version="1" fragment="single">IGKKLGDSHNNQLSPAKIRVVLCAMSMSLMFAFIDQNGISVVLPFIANDLNAQLTISWAGTSQLIANCCFQLLCGRFADIFGRKNVLVFMILMLGFFDLACGLAQTDVQFFIFRALCGVANGSVMSLVMVVMSDHVKLEDRGKYQGYLTAFIGIGNGVGPLLASAFVQHSNWRNYYYTLFALIVASSSIIIIFIPNSKSPISTREKLKNIDYLGWVFGAIGLILILIPINGGGLTFKWNEPKVIIMFVIGGVSFIGFFITEMWIAKLPLVPFRVFRSTSLAIIFAQSFFSGVAFYPLFYYYAYYFEVVRNKSVLITACYSLCVVGPHSTISAISGRFVSWTKHYNPTLWTGFALWTLALSLLAGALAKNTNLVGVAFIMVVNGLGQGMTFQNTLVAALAHSRSQDRAVVISTRNVLRSFGGSFGLAFSALIFSTTFTKKLQNDSFINQSDNATLKKYLQQHVYAKIDYSQISINDEQHEYIRDLYMKCCKDIFTFWAPVVGLCFILSWLIRDRGLVSKTDQKDRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P930</accession>
    <name>A0A1E3P930_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_90976</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040659.1">
        <property type="nucleotide sequence ID" value="XM_019186269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P930"/>
    <dbReference type="STRING" id="683960.A0A1E3P930"/>
    <dbReference type="GeneID" id="30203515"/>
    <dbReference type="OrthoDB" id="271725at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061574">
        <property type="term" value="C:ASAP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15481:SF0">
        <property type="entry name" value="LD23870P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15481">
        <property type="entry name" value="RIBONUCLEIC ACID BINDING PROTEIN S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="11"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="200" mass="22553" checksum="CCA5B9BDE2C9756B" modified="2017-01-18" version="1">MMNPIDQPVSTTLYVTSLPTMFNELHVRDLFKKFGEIVSVRKQGKGTTFAHVTFEKPSSAKRALKTLHGCQLKGRALKLRYAYHRQDREAILGGKKLIGSPQELLKNSPVYQELVNDKKSTPRFSAKKRAPRLWSALKPDLGNIIVPNSSLKLPGVNSYSSSETVRAPKTYNVENDPFPKTDQRVMEKFGLDADMGNKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9A3</accession>
    <name>A0A1E3P9A3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">amidase</fullName>
            <ecNumber evidence="3">3.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_82059</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a monocarboxylic acid amide + H2O = a monocarboxylate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35757"/>
            <dbReference type="ChEBI" id="CHEBI:83628"/>
            <dbReference type="EC" id="3.5.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amidase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.4" evidence="3"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041097.1">
        <property type="nucleotide sequence ID" value="XM_019185325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9A3"/>
    <dbReference type="STRING" id="683960.A0A1E3P9A3"/>
    <dbReference type="GeneID" id="30202571"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF4">
        <property type="entry name" value="AMIDASE C550.07-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="7">
        <location>
            <begin position="106"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="5">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="258"/>
            <end position="261"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="570" mass="62806" checksum="49450ED859ACEAC3" modified="2017-01-18" version="1">MSAAKLFIDHSKEPLLAPATSYKPNLELYEKEWVPKIKAYNEKLSNAIPNEYLAPSTLLPDDLDATPFNAMGIPATVLDEKTLTITELNASEIAQKIAARELTASETILAFIKRATLAHQLTHCAMEILFDYGIKRAKVLDEYQAKTGHTVGPLHGVPLSLKEHYEFEGHVTHAGYVSLLDNVSEKFSLTSQLLYDSGAVFYIRTTEPQSLMHMDSINNITGRGRNPHKTTMSPGGSSSGEGAIIAMKGSPLGLGSDIGGSIRGPAAFNGVWGLKPSSKRISLIGSISAREDTTCEAIQAVLGPLSNSVDDLELFMKSYLAQRPWELDQQLFPLPWKEVPTPRASDLTIGFVYDDGVVKPHPPVIRALKEVEEKLKAAGVHIIKWDPHRVYDAVEIANAAYSSDGNSAVFDRLEKSGEPLAPLSEHYLQIGKGDKGLSSMELQYYTHSREVLRQEYLKLFNKRGVDFLITPTYVGTAPKPSTIKYWGYTSLWNIMDQTCVTFPSGVFGDKSTDFKDESYKARNVYEEHEYGIYDPEASDGLPVGLTLVGRRYTEEAALKAAKVVSDIISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9A8</accession>
    <name>A0A1E3P9A8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">acid phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_65918</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histidine acid phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="3"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040749.1">
        <property type="nucleotide sequence ID" value="XM_019184024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9A8"/>
    <dbReference type="STRING" id="683960.A0A1E3P9A8"/>
    <dbReference type="GeneID" id="30201270"/>
    <dbReference type="OrthoDB" id="6509975at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016274">
        <property type="entry name" value="Histidine_acid_Pase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963:SF18">
        <property type="entry name" value="ACID PHOSPHATASE PHO11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000894">
        <property type="entry name" value="Acid_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00778">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133739" description="acid phosphatase" evidence="8">
        <location>
            <begin position="20"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="64"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="260"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="410"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000894-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="463" mass="51800" checksum="C4A32D636743B362" modified="2017-01-18" version="1">MKFSSILPLFSAVITIVEAASAPQTKPYSQDSLEQFDLLRYTGTTGPYVSHRGFGIDPEAPYQCEVTQAHLFMRHGERYPTKGTGKGEKKVFEKLKNATVEVYKGPLSFIQDYEFFVKDNNRLETESDKGFYSGLGDCYNLGANLRAKYDHLFDSKSVHPVFSSGQERVVDSARAFAKGFFASNYTDLASIQIIPENETQGVNSLTTHDACVNYNGSFSDELVSSFTGNEYLERAAIRLNSLSPGFNITASDVYDLLGYCGFELNVRGESKVCEIFTQEEFINFAYSKDLGFYYSNGPGYNLSVPLGSVYANNTYTLMKQGKEYPYNLTFSFSHDSDIITYTTALGIFEPDYDLSLNEVEFGSIFRSSEIAPMGGRIITERLDCMDVLTNETDAFVRVIVNDAVIPIPGCQDGPGFTCSLDGYKKNFEQRPGNTSYVEACGVNKTYPQHSSFYWDFEVTFEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9I2</accession>
    <name>A0A1E3P9I2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_75756</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040754.1">
        <property type="nucleotide sequence ID" value="XM_019184253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9I2"/>
    <dbReference type="GeneID" id="30201499"/>
    <dbReference type="OrthoDB" id="410267at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF319">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="287"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="340"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="376"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="34"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="444" mass="49164" checksum="463725F53893F767" modified="2017-01-18" version="1">MSESNEATLRERKDAQEDNNFEHDVEEFPEGGFGWLVVLAEWLIMACTFGMVNSFGIYQTYYQTQAYPDETAFKISVIGALQPFCIYIFAIPTVTMLHYLGSRVTVGIGALIQVFSFMMMSLTTDLWQLFITQGLLFGMGGGIIYLSGMTVVMQWFKKRRAMAMGIVSSGSSLGGVYWPIAIRRLINEVGFAWTNRIIGFIYIPLGVLMVVFLKSRLSLKHDIPKGSGKIKRVFMLKLSVLNNWRFLVLTFSNLIGMFALFPGLFYIDLYAGKLGIKVGSYLFNPDYILSVLNAASLVGRIVPGIMADYFGRMNVLFPFLILSGVSPLALWLTSENSNGLMIAHVVLFGIGSGVFVSLFPTCVTQLFGVQDNQSRISLFFAIGGIGALFGPIIGGSFLPLQDRESGNLDGFNKLAIFVGVMMLGCAAVELFLRVVYSKKAFQFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9J1</accession>
    <name>A0A1E3P9J1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_27336</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041286.1">
        <property type="nucleotide sequence ID" value="XM_019181360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9J1"/>
    <dbReference type="STRING" id="683960.A0A1E3P9J1"/>
    <dbReference type="GeneID" id="30198606"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000141">
        <property type="entry name" value="C2H2 zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000736">
        <property type="entry name" value="Zinc finger protein 423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF11">
        <property type="entry name" value="ZINC FINGER PROTEIN YPR022C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="72"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="128" mass="14703" checksum="63D4F23A43243B90" modified="2017-01-18" version="1">MTSSNAPTQQQQPLPPRESISSSSSLPTLPEEGSLDKLNFTPQPPADYQPPLHEPTRRGRKPSLAYDATKQFVCTYCSRRFRRQEHLKRHFRSLHTNEKPFNCELCGKKFSRSDNLAQHIKTHGNDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9P7</accession>
    <name>A0A1E3P9P7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proteasome alpha-type subunits domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_26574</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041346.1">
        <property type="nucleotide sequence ID" value="XM_019181279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9P7"/>
    <dbReference type="STRING" id="683960.A0A1E3P9P7"/>
    <dbReference type="GeneID" id="30198525"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03753">
        <property type="entry name" value="proteasome_alpha_type_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000015">
        <property type="entry name" value="Proteasome subunit alpha type-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033812">
        <property type="entry name" value="Proteasome_alpha_type_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="9">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ62139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="254" mass="27875" checksum="5C410D84D6E0DC49" modified="2017-01-18" version="1">MFLTRSEYDRGVSTFSPEGRLFQVEYSLEAIKLGSTAIGIATSEGVVLGVEKRVTSSLLESSSIEKIVEIDKHIGCAMSGLTADARSMIDHARVSSLNHNLYYDEDIGVETLTQSVADLALRFGEGASGEERLMSRPFGVALLIAGIDKEKGPQLYHTEPSGTFYRYEAKAIGSGSEGAQAELQNEYHSSLTLKEAELLILKILKQVMEEKLDENNVQLSSVTESEGFKIYENNVTADIIKVLKEQETDDQPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9S1</accession>
    <name>A0A1E3P9S1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PPP4R2-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_66461</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPP4R2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041372.1">
        <property type="nucleotide sequence ID" value="XM_019184042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9S1"/>
    <dbReference type="STRING" id="683960.A0A1E3P9S1"/>
    <dbReference type="GeneID" id="30201288"/>
    <dbReference type="OrthoDB" id="341898at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030289">
        <property type="term" value="C:protein phosphatase 4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015267">
        <property type="entry name" value="PPP4R2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16487">
        <property type="entry name" value="PPP4R2-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16487:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09184">
        <property type="entry name" value="PPP4R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="194"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="236"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="298"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="37621" checksum="CAC3146381BF2786" modified="2017-01-18" version="1">MSEPKFDLNKELETIIRERIFPKDHESLWKDIRTEIPTLIHTIITEFYEKPITTTIKDPLDSNHTESLESFSNRLTEHLTKVFPDNPPFTIVRLSEILYSPRKHYHTAEKFLRALENVIQVSSSIAEFPETTFEVNGHNSLKNHEFGENTLPISIGNDETDTIFLTKIPWLTEDDIKEIESEHYLIEEYPINDAPLNEEYDHDEQPLDGEQKKRTLEESKESPEKKHKPSEDSVEEEVHAGKETEKDKNEEEERAEAKQQKDEDERDTEKKEDESDSPKDKKDLEEPTKAEPDVSLIEADETLDEDKMDINTTLEEDKMDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9S5</accession>
    <name>A0A1E3P9S5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS7m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_25747</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041373.1">
        <property type="nucleotide sequence ID" value="XM_019181184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9S5"/>
    <dbReference type="STRING" id="683960.A0A1E3P9S5"/>
    <dbReference type="GeneID" id="30198430"/>
    <dbReference type="OrthoDB" id="9972728at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14868">
        <property type="entry name" value="uS7_Mitochondria_Fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.455.10:FF:000006">
        <property type="entry name" value="37S ribosomal protein S7, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047988">
        <property type="entry name" value="Ribosomal_uS7m_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="8">
        <location>
            <begin position="21"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="165" mass="18893" checksum="53135E3E5CCC7590" modified="2017-01-18" version="1">MEKFGIEPTPEQIAKAESLAEKPIPLKKDPIVDHCTNMIMKDGKKSRAQKVMGQALYIVRLQLRKDPVEVLREILEKMAPLMAIRTHKTRAAKSVVLPVPLTERQRHRTAFLWILEGAEKRRSPDLSVRLGEELIAAYEGKSSGYEKRLQIHKTAMLQRAYVKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9Y0</accession>
    <name>A0A1E3P9Y0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TFIIS N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_38292</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transcription factor involved in RNA polymerase II transcription regulation. May function in both SPT15/TBP post-recruitment and recruitment steps of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IWS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041408.1">
        <property type="nucleotide sequence ID" value="XM_019182534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9Y0"/>
    <dbReference type="STRING" id="683960.A0A1E3P9Y0"/>
    <dbReference type="GeneID" id="30199780"/>
    <dbReference type="OrthoDB" id="21124at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034243">
        <property type="term" value="P:regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051037">
        <property type="entry name" value="RNAPII_TF_IWS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46010">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46010:SF1">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="4">
        <location>
            <begin position="49"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00037992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="228" mass="25879" checksum="28CB7EDD81D4E023" modified="2017-01-18" version="1" fragment="single">MDVAAKKDAELIENQSGIAINKIQLLPKVRDILSKANLAEVILDNNLLAEVRQWLEPLPDASLPSYEIQKTLFSALLKLPINTNHLRESGIGKILLFYQKSKRVEPKIKRVADKLISDWTRPIIGASDNYRDKQFHSIDYDALKHKKKLKTSAEKIHIDNSEKSIYEQQAARRNRAAAPVPTVTDYKFAPKSRLERAPQSIMNAGVGTSLNRDDQFKRINQRLTGRKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA40</accession>
    <name>A0A1E3PA40_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_76441</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041468.1">
        <property type="nucleotide sequence ID" value="XM_019184382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA40"/>
    <dbReference type="GeneID" id="30201628"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32212">
        <property type="entry name" value="CYCLIN-LIKE F-BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32212:SF248">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="647" mass="75374" checksum="107CC9E1F8EE7D54" modified="2017-01-18" version="1">MDGSQAAKLPAKPVDITHLPNELLVKILHELDLTTTQSLFQIKELRNKLKVHMDIVVYSKSKFHDYFHSIPLSIIYESKRFNPLNSTSWITHYCLPIYKFFHGVARLHVEQDYRNFKILTLASGLSSLSYHPLEYKLVAEFFQQVYAKVVNWPLLQRITFKNITEVELLYQDLDFKLLEFPDALQIKFLNCTLVDNILDWEVQFPKLQELSLIDDGAEDILSCVDFTSSPLKRLYMESLDQRIVIKDLVFGYKTSDLSIETHHRNYRLTTAGGLSITKLENIFMPNVRELIVDQIFEIQNIDAPQLEVFEMSLKYEHLGFVRRVKLGKINAPNIEVFSFANGVMEIDQNFTDNIPIIDRFRITNGKVGLERSCNDNLKAKSIEIYIQQGAFETFDAEDWFESINLYTVEGLTFFIKLETKKPALVTNLNRLEFPKLKSLYFPDCSKFVSVPEVQAPSLETLRIAHANLSFRNFKRMVETFNNLKNLELKGVIFNNGSRNPLTYSPMDRNDPRLGFKNCSLPNLQRFRMSSDMDESIRPGFGFQNLYAPNLKKIDITFPALRSIDLNEFPLLEDVKIDGPLYIDLEQLKNLKSVLIQSDVLIELKLNEEMFLFPHQLTAFHVRCFPEKNTDAIVKLFKEKNRYRRFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAB3</accession>
    <name>A0A1E3PAB3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nitrogen regulatory protein areA GATA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_60404</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041550.1">
        <property type="nucleotide sequence ID" value="XM_019182985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAB3"/>
    <dbReference type="STRING" id="683960.A0A1E3PAB3"/>
    <dbReference type="GeneID" id="30200231"/>
    <dbReference type="OrthoDB" id="5563539at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007039">
        <property type="term" value="P:protein catabolic process in the vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052292">
        <property type="entry name" value="Glucose_repression_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28051">
        <property type="entry name" value="PROTEIN MTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28051:SF1">
        <property type="entry name" value="PROTEIN MTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="143"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="29096" checksum="DDBCFA2453A75D1E" modified="2017-01-18" version="1">MRYSFDDQYNVVTKKFSHLSSSNDHLITEFAIDYFQYQFDEIEIHNCWKIIHNANTQNNYNIPQSTDIDRLENISWRKWAKMKFDLEEVPPEMVNWYKDCDITWLYGPLAGSSSSLSHQVFQIEPPTSSSTAYGFDRTGLVPSRTSSFSSMTSASTAASLDDEHEHGLGGKNEDIFVDEHYLDNIGHQCSYAKPILKNKRATSLGFLEFEAPSSVVTKKRTASSNSGKKYNKSVSFDAIVRIRQFEIEEEACYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAF0</accession>
    <name>A0A1E3PAF0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YMC020W-like alpha/beta hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_87857</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041590.1">
        <property type="nucleotide sequence ID" value="XM_019186130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAF0"/>
    <dbReference type="GeneID" id="30203376"/>
    <dbReference type="OrthoDB" id="5598028at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058933">
        <property type="entry name" value="YMC020W-like_ab_hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26147">
        <property type="entry name" value="AB_HYDROLASE_YMC0-YMC35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YMC020W-like alpha/beta hydrolase" evidence="1">
        <location>
            <begin position="53"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="213" mass="24805" checksum="61595FB5FA705675" modified="2017-01-18" version="1" fragment="single">PDAIWGWYQNHYNDRKDGELSVLDTKTETNPVDLNKYPGQYQEPEGNEGCVVPDFMECFREITTKTKVRIATELYYNYPTEKHLYIKRKIHKPLIRYVLVVSVMGGSKGSKANVVNSKTLSSLAVQSIKDWYTDKETSYDYQIESVSLEASKLTENTSEDLFKLLINWREQFKKIDHVFFVGYSTSVPLSAKLLEIMISRNLFDNSKNYWNIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAT2</accession>
    <name>A0A1E3PAT2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha N-terminal protein methyltransferase 1</fullName>
            <ecNumber evidence="5">2.1.1.244</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Translation associated element 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">X-Pro-Lys N-terminal protein methyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_25457</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54712"/>
            <dbReference type="Rhea" id="RHEA-COMP:13785"/>
            <dbReference type="Rhea" id="RHEA-COMP:13971"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138057"/>
            <dbReference type="ChEBI" id="CHEBI:138315"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-terminal L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-methionine = N-terminal N,N-dimethyl-L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54736"/>
            <dbReference type="Rhea" id="RHEA-COMP:13787"/>
            <dbReference type="Rhea" id="RHEA-COMP:13974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138059"/>
            <dbReference type="ChEBI" id="CHEBI:138318"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-terminal L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54724"/>
            <dbReference type="Rhea" id="RHEA-COMP:13789"/>
            <dbReference type="Rhea" id="RHEA-COMP:13973"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138061"/>
            <dbReference type="ChEBI" id="CHEBI:138317"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. NTM1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.244" evidence="5"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041685.1">
        <property type="nucleotide sequence ID" value="XM_019181148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAT2"/>
    <dbReference type="STRING" id="683960.A0A1E3PAT2"/>
    <dbReference type="GeneID" id="30198394"/>
    <dbReference type="OrthoDB" id="1298661at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071885">
        <property type="term" value="F:N-terminal protein N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000025">
        <property type="entry name" value="N-terminal Xaa-Pro-Lys N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008576">
        <property type="entry name" value="MeTrfase_NTM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753">
        <property type="entry name" value="AD-003 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753:SF0">
        <property type="entry name" value="ALPHA N-TERMINAL PROTEIN METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05891">
        <property type="entry name" value="Methyltransf_PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016958">
        <property type="entry name" value="DUF858_MeTrfase_lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="127"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR016958-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ62478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="233" mass="26160" checksum="40C4E7EFB6106849" modified="2017-01-18" version="1">MSEAEDNRADALIDYNDAIDYWTSIPATVDGVLGGYGEGTSVPKADVVGSSTFLRKLKARMTPEKGQIKYSVDIGAGIGRVTRDMLHKFSDKVDLVEPVKPFVEQIHQELKPLKDEGKLGEIYDIGMQEFIPEEGKYWLVWCQWCVGHLPDKEFIEFLIRCKNGLQPNGTIVVKENNAQGDDVFDETDSSVTRSNANFQKLFKKAGLKLIATELQRGLPKELFAVRMYALKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAX9</accession>
    <name>A0A1E3PAX9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_25149</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041776.1">
        <property type="nucleotide sequence ID" value="XM_019181107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAX9"/>
    <dbReference type="STRING" id="683960.A0A1E3PAX9"/>
    <dbReference type="GeneID" id="30198353"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000384">
        <property type="term" value="F:first spliceosomal transesterification activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17945">
        <property type="entry name" value="DEADc_DDX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="9">
        <location>
            <begin position="177"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="208"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="422"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="177"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ62569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="575" mass="65784" checksum="1CB9E1A4A447A724" modified="2017-01-18" version="1">MSERGPISLEELIAKKKSEGKLETPKFLSKKERQKLALERRQNKVNQIQSNNASHRFKPYDIEKAEGKKDSFDENFSKNYEKVLERARTGNKFNFEWKNDDDTLNNTDSIASQYASYDLSKPRRDDIGYAKSKSKKNWDDIHWSEKPLENMKARDWRILKEDFEIITRGSNLPNPLRNWKESKIPNELLDIIEKLGYKDPTPIQRASIPISLSKKDIIGIAETGSGKTLAYLIPMLSKSLKLPRLNEFTKTDGPYGLILVPTRELAQQIETEFKKFSKYLPDIDIISIVGGKLIEKNILDLQNRTIEIIIATPGRLIDCLERHYLVLNQINFLVLDESDKMIEMNFGDQVAKITEFMNTKRQNMMFTATMTSDVEKLSKSYVNDPAIVNIGNVSSSEMTINDRIEQRFEFFNSNDDSKKANKLTRILSSGQYPAPIIIFINYKESGEYLFKNLTQSGFKVALIHGSKNQEQREFAIKQLKEGKADILIGTNVASRGIDIPNVSLVVNFQMSKKIDDYIHRVGRTGRAGSYGTSITFLSDEADSEIYGDLKKLVSKSGNKLPEEFKKINDKVQNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB13</accession>
    <name>A0A1E3PB13_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Choline/carnitine acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_60635</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041788.1">
        <property type="nucleotide sequence ID" value="XM_019183029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB13"/>
    <dbReference type="STRING" id="683960.A0A1E3PB13"/>
    <dbReference type="GeneID" id="30200275"/>
    <dbReference type="OrthoDB" id="240216at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004092">
        <property type="term" value="F:carnitine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000019">
        <property type="entry name" value="Carnitine acetyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000003">
        <property type="entry name" value="Carnitine acetyl transferase FacC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF29">
        <property type="entry name" value="MITOCHONDRIAL CARNITINE O-ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="7">
        <location>
            <begin position="13"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="531"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="719" mass="82005" checksum="E1C9F7D1A8F3765C" modified="2017-01-18" version="1">MSTFQYQDKLEKLPIPDLKSTCENYLNALRPLQTEAEFKETQAATHKFLNGIGPYLDQQLREYSKTRPSYIEQFWYDSYLNYDSPVVLNLNPFFLLEDDPTPINNTQVKRATSLTQSSLEFIRALRREELPPDMIKTTPLCMDQYHKLFGSARIPTQTGCVMQSDSSSHHIIVLSKSQLYWFDVLDRNNDLILNDDEMSINFESIVRDSMKTTPSELARSSFGVLTTENRKNWANLRNSLLNDPINYKILKIIDSALFVVVLDDIELDTLPDLSKNMLCGISKLEKGVQVGTCTNRWYDKLQLIITKNAKAGVNFEHTGVDGHTVLRFCSDIYTDSILKFAKSINPNSPSIWKSKSPSILNKSSIDTNIITIPRKLEWNLTSDLSLSLRFGETRLSDLISQNEFATLEFKNYGSDLIKKLKCSPDAFVQMSFQAAYYALYGKVECTYEPAMTKQFLHGRTEAIRTVSLESNLFVRRFFEDVPGKDKLEFLRIASKKHSETTRRCSQGLGQDRHLYALFCIWKRYVADHDDSNADDTDGEDGDTHSDSTIGNSESMASFGGKFDKNEIPSIFADSGWDKLNNSIISTSNCGNPSLKVFGFGPVASNGFGIAYIIKENSITVCVSSKHRQTQRFIDTLKNYYEEIEHIYKEEILNAEKQKERGRLQHARSGNLNYLLGGYGYFDLEDDEIKSRGNSPERFGFKKSGFSSRQLSEKLRLAEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB92</accession>
    <name>A0A1E3PB92_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mediator of RNA polymerase II transcription subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mediator complex subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_76661</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the Mediator complex subunit 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041701.1">
        <property type="nucleotide sequence ID" value="XM_019184430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB92"/>
    <dbReference type="STRING" id="683960.A0A1E3PB92"/>
    <dbReference type="GeneID" id="30201676"/>
    <dbReference type="OrthoDB" id="5310959at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019680">
        <property type="entry name" value="Mediator_Med1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10744">
        <property type="entry name" value="Med1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med1" evidence="8">
        <location>
            <begin position="12"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="381" mass="44532" checksum="76650F9F2B8E6CED" modified="2017-01-18" version="1">MQDTFHQKLSSIVKILKSSPGSVTVQNIQRLSNIHKFETFIETINDKVKRLSIAGKILVMDIDFIELPSKNKDQTRFELNDVRLILANNNNSFKYVNTENGENILKNCLLNYPDLSKFDETLETLSIFDQVSHDDFDLFDYYMKIYDAFHQIPEFDVKMNTNDKFTIIVQDMFEVSLVNDERLNLSRIEYNGEWNDKKQKINDVGLQVKFIGDVSIAITKNILLQNDLIFDDSKKKKLFDSNEVFKSKKNSNLTFQGFQFLESEVLLISEVFSNDLPSLMKVLVELHKFSKLERFIEQLKQNFTLIDKFADLENEDVRLNDFLNGESIDEIEVEHHFITMSLKQQQIELKSDDSEYNFTIDTSIQHKLDEGLEKLKGILEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBE1</accession>
    <name>A0A1E3PBE1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_88494</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041932.1">
        <property type="nucleotide sequence ID" value="XM_019186156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBE1"/>
    <dbReference type="GeneID" id="30203402"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="485" mass="55773" checksum="97F883DD9F7D1C75" modified="2017-01-18" version="1">MSQQSKSREERFQKYTNRHQVPVHPLESDTSTTIYHDALELQGDNSDTANSDTTITETSSHPTEFTSSNFETTPTTETEMPQSTTADALANLFQQVQSLTIQLSEVRESVPINEIQPDITSTLKRFFPSLIIEENKVLGTKLLSEISGSQIVVKENELIRQFDSNICQQLLNFQTRAGFLQLSFSQYGNFLYQFLDSYNKAVINKNTNIQDEERNYNYLQMLKTLLQDYDFHREEITRFQKLLQCEPVIGQTVSGYFETLFQMSRDVHSFSSYSVIQFRVIKILDLIIPNLNLHQFFIIQNRNEFYTLLSRYNLSQHKFTAELINQLNSSPQLVNTSTTAPPTSFPNSAITYAVNPSIKRFNKSNNNKSTEPSKRKFKNYFCKRCSCSRCEINKKLYDDYIKRKPASNAVVHVIYQDDLLDAEIELIPNDQLHFEEADDVLGIEEVPPPSDAASALDDETQEQQVLYEITANAGLTSNTNYRPIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBJ0</accession>
    <name>A0A1E3PBJ0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YEATS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_38991</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041791.1">
        <property type="nucleotide sequence ID" value="XM_019182541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBJ0"/>
    <dbReference type="STRING" id="683960.A0A1E3PBJ0"/>
    <dbReference type="GeneID" id="30199787"/>
    <dbReference type="OrthoDB" id="1741717at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16905">
        <property type="entry name" value="YEATS_Taf14_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1970">
        <property type="entry name" value="YEATS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038336">
        <property type="entry name" value="NET_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016665">
        <property type="entry name" value="Sas5/TAF14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038704">
        <property type="entry name" value="YEAST_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005033">
        <property type="entry name" value="YEATS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055129">
        <property type="entry name" value="YEATS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23195">
        <property type="entry name" value="YEATS DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03366">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016551">
        <property type="entry name" value="SAS5/TFIID_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51037">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YEATS" evidence="4">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="139"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="244" mass="27521" checksum="140D5A5C222FA81D" modified="2017-01-18" version="1">MAEVQRTIRIVTNQNIVEGAPVPAEGFPLRAWSIEIFLLNSEGKPIPATIFEKVTYHLHPTFANPTRVLKKAPFKISEEGWGEFELGLTLTFIEKSGERKLAHDLNFQQNRYEVDHVLNVPINKSANLRNLLAESGSVPDVDENIGDKRKQNNANDSKNKRSKLASSSMTNPIKGSVDLEKLAEGLTKLNEDDLLGVVQMVTDNRTPDMNIKNDVEEGEFTMDLYTLPDSLLKSLWEYVKKRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBN6</accession>
    <name>A0A1E3PBN6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial ornithine carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_49726</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041504.1">
        <property type="nucleotide sequence ID" value="XM_019182638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBN6"/>
    <dbReference type="STRING" id="683960.A0A1E3PBN6"/>
    <dbReference type="GeneID" id="30199884"/>
    <dbReference type="OrthoDB" id="2139348at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000109">
        <property type="entry name" value="Ornithine carrier protein AmcA/Ort1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF31">
        <property type="entry name" value="MITOCHONDRIAL ORNITHINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="9"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="110"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="216"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="33442" checksum="C4767A5BEE626573" modified="2017-01-18" version="1">MTDSEFSLKNSLKDILFGSLSGCTGKLIEYPFDTVKVRLQSQPHDQPLKYKNSFDCIKQTFTNEGFFGFYRGISSPIVGAAAENACLFVTYNIAQDFLKNQLSMKQNEELPLNLLVLSGGFSGCVASYILTPIELIKCKLQVEQVYSQKKSSIIQLIKQVLTNDGIKGFWHGQTGTLLRECGGTASWFGAYEFMSKELKIARLGSENINGAENTISELLISGACAGISYNLSLFPADTIKSKMQTSSVVNPDQKLTFISTAKLIYKHGGLKAFYRGLGITLTRAVPSNAVIFFTYEKLKKTFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBX2</accession>
    <name>A0A1E3PBX2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">60S acidic ribosomal protein P1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_82494</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). The 5 acidic ribosomal P-proteins form the stalk structure of the 60S subunit. They are organized as a pentameric complex in which uL10/P0 interacts with 2 heterodimers, P1A-P2B and P1B-P2A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041669.1">
        <property type="nucleotide sequence ID" value="XM_019185388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBX2"/>
    <dbReference type="STRING" id="683960.A0A1E3PBX2"/>
    <dbReference type="GeneID" id="30202634"/>
    <dbReference type="OrthoDB" id="2194681at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05831">
        <property type="entry name" value="Ribosomal_P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696:SF10">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="72"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00063629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="11097" checksum="11F4E0411B5584B5" modified="2017-01-18" version="1">MSVESSLSYAALILADSEIEISSEKLLALTTAAKVPVDKIWADLYAKALETQDIKELLTNISAGGASAGVAAAAPGAAASTEEAAEEEKEEEAKEESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCP5</accession>
    <name>A0A1E3PCP5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SYO1-like TPR repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_27602</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nuclear import and ribosome assembly adapter family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041864.1">
        <property type="nucleotide sequence ID" value="XM_019181392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCP5"/>
    <dbReference type="STRING" id="683960.A0A1E3PCP5"/>
    <dbReference type="GeneID" id="30198638"/>
    <dbReference type="OrthoDB" id="288703at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13394">
        <property type="entry name" value="Syo1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052616">
        <property type="entry name" value="SYO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057990">
        <property type="entry name" value="TPR_SYO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13347">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13347:SF1">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25567">
        <property type="entry name" value="TPR_SYO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SYO1-like TPR repeats" evidence="3">
        <location>
            <begin position="383"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00049983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="603" mass="68515" checksum="045282D5C64545A2" modified="2017-01-18" version="1">MGKLKKRSRSSRHRVNPLSNQSKDERLDASLRESKILPIIAKLKSVVPNDKSMALGTISVMCEDYKLRNMLLKEKLLQLVMEQCLNDENSEIIVESFGLLRNLVIEEGYDVAMYLWRQNIWVTIESNFEKAKVSFDAIQKDGQKFNKNESRLLFDYIENLISLIVGLGNASDDIFEQISNKVDPLINFIKEILTFGVDLESKSLKTSVSLFNSILDLIYDFSSQSIDFINGLNQTWNIDIELLERFLKDANLNKLPGVYIQGIKLQILEASESTTNKDDVVAEIINKIISEVQDINIENARNTLIEKVDNTQNVVVADDTKKRATAKAELQAIALAIELITAAIEIIAVTKMANDDIDPDTQNDALLDCLLNRVPEVLIHLLKYDEFRTTSLTALNNLSWLFATLRVALDDWALVAKNLWGVVTENLGSDIENKISSFGILWAIISVVHEQINIDDNFAKSVFTEFQNTLKDTTIDNDLKEEYLVRLVGFLGTLAQFQGNIERNAEISQFLFQVLNSLPNLPSKVAIEILDSIYEIYGDAEYDYDEPVFVQRNYLNSLQQLTPKIKHAVKLVDKNVDPELKAKGEDVFVNLSRFIDYKKKERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PD28</accession>
    <name>A0A1E3PD28_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_88594</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041994.1">
        <property type="nucleotide sequence ID" value="XM_019186168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PD28"/>
    <dbReference type="STRING" id="683960.A0A1E3PD28"/>
    <dbReference type="GeneID" id="30203414"/>
    <dbReference type="OrthoDB" id="1937934at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="2">
        <location>
            <begin position="96"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="166" mass="18592" checksum="0EA3B702EA64025D" modified="2017-01-18" version="1">MSSLAILLENVAGISKQQLLKDFPPEQIPVIGLQNGEFIRSETNQRLLDRSKQSLAKYLSQDYKGERPQQVDPKIHTQKQIEEAIKESQKKSKTLETLKHAIDVPVDFISSIIGKNGLKITEIRSKSNSNVIIDDQIKNNLYKVVTITGLPESNLIALQYIAGLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PD49</accession>
    <name>A0A1E3PD49_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_50734</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019182656.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3PD49"/>
    <dbReference type="GeneID" id="30199902"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="MFS sugar transporter protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF24">
        <property type="entry name" value="HEXOSE TRANSPORTER PROTEIN (AFU_ORTHOLOGUE AFUA_8G04480)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="356"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="423"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="453"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="483"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="60"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="541"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="548"/>
            <end position="561"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="561" mass="62760" checksum="FD7CB435AA42A92D" modified="2017-01-18" version="1">MVGIIADEVKFFKKNKQPVKKLPVNPGDVPDDDAQHLKHFLPNYGKAWFKVPHLLKLNLILLIVSLTSTNTGYDGSLLNAFQSIPDWRMAMNNPSGAVLGAINNGVVFGCILGFPFASPIGDRYGRRHAVTIGNIVMIIGTVIQSYSRAYAMFLVARIIIGFGNFISSVSSPSLISELSYPTHRQAVTAFFNSNWYLGAIIAGWVSFGTRNVGSNWSWRIPTILQGLFPVIQFFLIYLVPESPRWLISKGRNQEARDLLLKYHAGGDESVGGALVDFEMSEIELALEQERLAKKIADKSSYFDFLKTPGNRKRLFLTCFVAFVMQLSGNGLVSYYLNKVLNSIGITSPTEQLIFNGGLQIYNWGISIIINLLVFQNFRRRVVFNTSIAMMLFFYIIWTILSAINQERNFEDKSLAKGVMAMIFLYYLAYNIGLNGLPYLYMTEISTYQLRSKLMNINIFGQQVAGVYNGFVNSIAMDAIEWKYYIVYCCILAVELVVSYLTFVETSGRTLEEMAEVFGESIGDHGFTLAQKKNEVKHLEDGAMADDSGSGTSAEKSNNLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PDJ0</accession>
    <name>A0A1E3PDJ0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_89377</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ63022.1"/>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019186182.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="30203428"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ63022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="50" mass="5663" checksum="5BA1A9DDEDEFFEBC" modified="2017-01-18" version="1" fragment="single">MSMMLGTLMDSYNSKAHNSTFQGHHVNMIFLTQNILLEILNKSNIVLSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU91</accession>
    <name>A0A1E3NU91_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">14-3-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_86209</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 14-3-3 family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ56749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035956.1">
        <property type="nucleotide sequence ID" value="XM_019186013.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3NU91"/>
    <dbReference type="GeneID" id="30203259"/>
    <dbReference type="OrthoDB" id="10260625at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050815">
        <property type="term" value="F:phosphoserine residue binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030437">
        <property type="term" value="P:ascospore formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031397">
        <property type="term" value="P:negative regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070873">
        <property type="term" value="P:regulation of glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001402">
        <property type="term" value="P:signal transduction involved in filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11309">
        <property type="entry name" value="14-3-3_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.190.20:FF:000002">
        <property type="entry name" value="14-3-3 protein epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00797">
        <property type="entry name" value="1433_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="5">
        <location>
            <begin position="5"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="233"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="233"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="249"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="58"/>
        </location>
    </feature>
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        <location>
            <position position="131"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003466"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ56749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="261" mass="29549" checksum="5949BA06E956B49F" modified="2017-01-18" version="1">MSLSREDSVYLAKLAEQAERYEEMVENMKAVASSGQELSVEERNLLSVAYKNVIGARRASWRIVSSIEQKEETKGNEAQVNLIRDYRSKIESELTKICDDILNVLTQHLIPSAATGESKVFYYKMKGDYHRYLAEFATNDRRKEAADLSLESYKSASDVAVTELPPTHPIRLGLALNFSVFYYEILNSPDRACHLAKQAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMSETGQEEGSSQAPEEKPTEPKPEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUQ1</accession>
    <name>A0A1E3NUQ1_WICAA</name>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_98115</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ56858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUQ1"/>
    <dbReference type="STRING" id="683960.A0A1E3NUQ1"/>
    <dbReference type="GeneID" id="30203878"/>
    <dbReference type="OrthoDB" id="4092496at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025928">
        <property type="entry name" value="Flocculin_t3_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13928">
        <property type="entry name" value="Flocculin_t3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
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    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133509" evidence="4">
        <location>
            <begin position="24"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="187"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
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        <location>
            <begin position="199"/>
            <end position="249"/>
        </location>
    </feature>
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            <end position="279"/>
        </location>
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        <location>
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        </location>
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        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ56858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="440" mass="44314" checksum="E84B2FDF6F13AC71" modified="2017-01-18" version="1">MKLSTRFSVPLVLAASLVSKVSAVAIPEAIALPWAQANPQAYAAANAYADAYAEAVAIAHPDPEAYALAASADDCASISCHASCGLLIIEGQACSQNSENSYSGPYNTTCLCSSGSKFLQYYAPCMDCGWTLWKYYGGYVSSALAACETLSTEPTGTLRCSTTLTDSYSIDTGIQGCSYLGNCPTETSSTAQTTDSPDQTSAAPTSAEPTSAAPTSDAPSSTQAAPSTQQTDPAAPTSEEAPTAPTSSADEPNAPSSNEASAVPSNSNGEPSAPNSDANTSGSPAPGGATSGQEDPSALDPAASSTSAKFSGYTNGTTSTAIVSHGSTTLITVTSCDENKCTEIPSTAQVSIATTTINGVVTSYTTYCPLTTTTPLTTKASTDKQTTKAPEDVTSKVTATTTKTGASTLASTTPVISTFEAVANKLAGSVGAILMALLCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUS1</accession>
    <name>A0A1E3NUS1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial peculiar membrane protein 1</fullName>
        </recommendedName>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ56833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="4044171at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="Mpm1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17234">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ56833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="31848" checksum="B471978D96B04E5F" modified="2017-01-18" version="1">MGIFSNSQQDKDYTETQVDRTINNRFASPKDIAAVNDFFNNQVISPLGNIIDLDAISNVFNKSNFPFHDNVNEIIGGFTRGFGAGDESLTTGLKAYPTPSATLYQKCMDKDGLSVWDDKGWWRCLFPKLALDGEHVLSKEDVLDDKSNKFGLFFKDYTGYLDWKSQMKQLVKQKDEEQKLARHRDNIESIYNDYSKDSNFEFGQGGNDVISSSKSVYFKTLPNGDGEEVTEISNVFRDGKSENKKFKRLIPRDGEPSTIEYLPDDKNDSKLSSWIWGSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUV3</accession>
    <name>A0A1E3NUV3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nucleolar complex protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_65756</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOC2 family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ56874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUV3"/>
    <dbReference type="STRING" id="683960.A0A1E3NUV3"/>
    <dbReference type="GeneID" id="30201227"/>
    <dbReference type="OrthoDB" id="10266662at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030690">
        <property type="term" value="C:Noc1p-Noc2p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030691">
        <property type="term" value="C:Noc2p-Noc3p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005343">
        <property type="entry name" value="Noc2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12687">
        <property type="entry name" value="NUCLEOLAR COMPLEX 2 AND RAD4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12687:SF4">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03715">
        <property type="entry name" value="Noc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="128"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="670"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="597"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="91"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="144"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="670"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="685"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ56874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="709" mass="81741" checksum="15F19EEC8B990CBD" modified="2017-01-18" version="1">MGKTSKSTKKFQSKHLKRTLDHRKEVKKHKQLVDRRKKKNGGDQDGEARPAKSNEIFDDMNVEEFFEGGFEVPKEQKGKKTESKKQKKEAEEEDDESSSEDEEILEHQDDLSKLAEKDPEFYKYLQDNDKDLLDFNPTNPLDAISDDEDGEGEDDEEDQVEKKVSKKAQQDEESSDKIEVTVKLVKDWNKKLEEPSLKLIKNIVSAFKSAVNVNKENQEYKYTVTDPKAFQELIFLGLKKIPIAVQKLVPYKVVKNSRTVPKSTKVKQLNTILKSHAGSLLTLLNDITNTETAALVLSSVQELLPFFISFRRLLKELITAVVNVWSTTSEVETQIAAFAFLNNSSREFSKSILETVLKLTYSSFIKNCRQTNIHTMPMVNFQKNSAAELFGIDEPLAYQIGFDYIRQLAIHLRNSINNTKDGYKTIYNWQYAHSLDFWSRVLSVHCNPEREIKKESPLRQLIYPLVQVTLGAARLIPTAQFFPIRFYLIRSMIRLSQNTGVFIPLFPLLQEILNSTTMTKNPKNSNLQAFDFDHNIKANSAYLGTRMYQQGVCEQFVDLVGEYFVLYSKSISFPELATPVTISLRRYIKSSKNVKFNKQLSNLVEKLNQNIEFIQQKRANVEFSPTNRVEVNKFLIDFNWEKTPLGGYVVVQRQVKEERARILRESLLEDEEERKKAKEVKDMGGDSEDEDVDEAMEDLESSEAEEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUX9</accession>
    <name>A0A1E3NUX9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_20206</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the eukaryotic/archaeal RNase P protein component 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036208.1">
        <property type="nucleotide sequence ID" value="XM_019180861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUX9"/>
    <dbReference type="STRING" id="683960.A0A1E3NUX9"/>
    <dbReference type="GeneID" id="30198107"/>
    <dbReference type="OrthoDB" id="128536at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007175">
        <property type="entry name" value="Rpr2/Snm1/Rpp21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04032">
        <property type="entry name" value="Rpr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="114" mass="12750" checksum="7B410118605C08B0" modified="2017-01-18" version="1" fragment="single">PKKPVIANREGFSRASFLYHASQSMALQNEALSRMYSKSMDSVTKKSVLKLDPSLKRTVCKGCHRVQTAGVSCAITVENAGKQSEVYQVKCKCGKLKRYPVGKNRDYVLFSEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV36</accession>
    <name>A0A1E3NV36_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ammonium transporter AmtB-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_98145</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036107.1">
        <property type="nucleotide sequence ID" value="XM_019186634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV36"/>
    <dbReference type="STRING" id="683960.A0A1E3NV36"/>
    <dbReference type="GeneID" id="30203880"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015200">
        <property type="term" value="F:methylammonium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140157">
        <property type="term" value="P:ammonium import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF3">
        <property type="entry name" value="AMMONIUM TRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="285"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="9">
        <location>
            <begin position="27"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ56900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="476" mass="51809" checksum="81A5DFB5992D4975" modified="2017-01-18" version="1">MSLFKRVIENYDEEVASLVPADMAYVAVATVMILVITPGIALFYGGMLRGKNFMTLIIQSYMVTSLITIQFYLFGFSLACSTTSSSVIMGNFTFGALKNVSAGPLAEGGTIPAILTFTFNAIFAVCTVQIFLGAISERGRLLPSLVLGFVWCTLCYCPFAYWTWSANGWLYKLGSLDFAGGGPVHVSSGTASLAYSFFLGRRKGWIDGKPPKFKPYSPVLSFIGSLLIYFPWLFFNSGTLTTITTPRTLYILTNTHLAASFAMATFVFYDYAVTKKWSIQAAAEGLIVGLVFATPACGWLAPWAIVVGSIFTALVCRSTYNIHAWMGIDDTSHSFNVHALGGVLGSLCTGVFASPNIAAMDGVTEIEGGWIFHHWKQVGYQLAGDAAIISWTFIMTYALCFIIDKIPGLKMRVSEEAEEMGLDLYEMAETADPFIKGFEDNSHVEYLTGEQAGSSNSNLSNNIELGDVKRPVPAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV64</accession>
    <name>A0A1E3NV64_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_65351</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036296.1">
        <property type="nucleotide sequence ID" value="XM_019183895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV64"/>
    <dbReference type="STRING" id="683960.A0A1E3NV64"/>
    <dbReference type="GeneID" id="30201141"/>
    <dbReference type="OrthoDB" id="241990at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031567">
        <property type="entry name" value="CRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008828">
        <property type="entry name" value="Sin1/Avo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031313">
        <property type="entry name" value="Sin1_PH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056385">
        <property type="entry name" value="UBL_AVO1/Sin1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335:SF1">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16978">
        <property type="entry name" value="CRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16979">
        <property type="entry name" value="SIN1_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23164">
        <property type="entry name" value="UBL_AVO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIM" evidence="3">
        <location>
            <begin position="421"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="AVO1/Sin1 ubiquitin-like" evidence="5">
        <location>
            <begin position="605"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="domain" description="SIN1-type PH" evidence="4">
        <location>
            <begin position="785"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="90"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="336"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="719"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="126"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="205"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="239"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="337"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="369"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="389"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="743"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ57089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="897" mass="101932" checksum="FA931F66F3B40D08" modified="2017-01-18" version="1">MALVQDEKFLINYIRAEYLKIADDDQSRRIIRPYTTNETDYPDYPEQQLSQEFNEILTRTESPPIPINVLENTDLMRRLHMSKESLADRIDETDEEEQEPFDHNTLKKQTLESHKPDSKASLATLKFNQSQDTVPAQDPGTLTPQTSETSHIGKKLPSFANTKISYKPPFTKLFRNTSSSNSSAQSSMKSKKERKKPPSDRNQADDGDNETYDNFDEEDNESDDDDEDDDDEDSNFDDQTTTASQANTESDLVDLEAMEDNTASQRQDESGILVDQNYSDLNTASQMDEFNDSKYLYGDNSISSVDDQLLLDSDFSDEDEDDIIDNALLSARLHMMQSKTPSSSATPSRSNRKLSFEKQARPKLRQKRSSSLSVRNQDTSSNDLRHVHSNPNIPDMQRSTSENSIGPLFEKVTVPSEPKLKSQLSEMIYKKYKAENSDPLEYFLFVSGENISNRFDVINMTVYLPQDNTLKLKIRKTVTVFEAIGFILFNVTKQFPKMLKKNKALRNPNKWCLKLIDDDGEPYEGSFGLMDRTKVVSSYGEDEVALIEVSDVEFQSNEIKTPLPVSEAELGGSGTPSPSKSNAIKQTNYFTSIIPKIDNNIKDAATVKVEIYKYPANEDSSFTALEFPLTAKLNEILLKYTKMKGLDPNGYQLKVSGEDYVLDLNDSVSSLDGSYKLEILTKRKTRELQLKKKKILDNSTLPTINSNLTPQSLMVANELPNESDKPQPQSLGKPAVQRRHSFSSKQFSSSFSKHGISSSFKNMNNSRGSLRTPEDSMLPNAVTADYRKYTVWRRLPMSFINRHERSFAIDGEYVYILPKDGKIWYDTNFKTRTFHVSQIVSCKVSKRIPSNFKIVVLKSNGPKRYDFEALNPSEAAEIINRLHKLMEAYKVYNNSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVM9</accession>
    <name>A0A1E3NVM9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_65897</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454240">
        <property type="protein sequence ID" value="ODQ56637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035844.1">
        <property type="nucleotide sequence ID" value="XM_019184011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVM9"/>
    <dbReference type="GeneID" id="30201257"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ56637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="51" mass="5539" checksum="C83683F148321652" modified="2017-01-18" version="1">MKTTGSQISSHVQGGIIMENKGGNTAYSVEIVENLITFGDIFGTMKDFEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVN1</accession>
    <name>A0A1E3NVN1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_71164</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036428.1">
        <property type="nucleotide sequence ID" value="XM_019184234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVN1"/>
    <dbReference type="STRING" id="683960.A0A1E3NVN1"/>
    <dbReference type="GeneID" id="30201480"/>
    <dbReference type="OrthoDB" id="10254310at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048203">
        <property type="term" value="P:vesicle targeting, trans-Golgi to endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016342">
        <property type="entry name" value="AP_complex_bsu_1_2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002291">
        <property type="entry name" value="AP_complex_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="8">
        <location>
            <begin position="16"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="638"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="641"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="669" mass="75830" checksum="9B077CD86A12AF03" modified="2017-01-18" version="1">MSLEKKIKKMFQAPRKGETYELRAGLVSQYASERKDSIKQVIAAMTVGKDVSALFPDVLKNIATHDLKQKKLVYLYLMNYAKTHPELCILAVNTFVQDTEDPNPLVRALAIRTMGCIRVDKMVDYMEIPLNRTLTDENPYVRKTAAICVAKLFDLNREICIENGFLDKLQKLAEDSNPMVVANAISALAEIHESDPSLKVLEITSDVLKRFLMALNECTEWGRITILTALSDYETADKEEASHIIERVIPQLQHSNPSVVLAAIKVIINNVEKLKSNADEYEQILKKLAAPLVSLVSTPPEVQFVTLRNIRIIIEKYPAILTNYFKVFFVRYNDPLYLKLEKIEIIVRLANENNSGLILNELKEYGYEFDTEFVKRAVKAIGQVGIKLPKYAAKATEYLIGLINERELYDEVTIVLRDVLRAFPKNLGSTIVPTLVQIQDNLVDSEAIASFVWVLGEFDVVDFENKLSEYVDGFIELDLQIQSALVYSLVKINVKTGQLKELLAKIFNNVDAIENIEIRDQIYLYWRILSLDDSSELKKLILFKLEKINNTIPTFQPELLAFLLKELSTLNSVYFTTGVSKGTKTTQIKKIKDIQLTVHNEENLLDFDDDGSSNTANGEAQGESINILDELNDLFSSSTIQPQQQQQQQQQGNNNNTNNGISKDLLDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVP2</accession>
    <name>A0A1E3NVP2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Crh-like protein</fullName>
            <ecNumber evidence="18">3.2.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">WICANDRAFT_47880</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <topology evidence="3">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the glycosyl hydrolase 16 family. CRH1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.2.-.-" evidence="18"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036486.1">
        <property type="nucleotide sequence ID" value="XM_019182606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVP2"/>
    <dbReference type="STRING" id="683960.A0A1E3NVP2"/>
    <dbReference type="GeneID" id="30199852"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02183">
        <property type="entry name" value="GH16_fungal_CRH1_transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000162">
        <property type="entry name" value="Glycosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017168">
        <property type="entry name" value="CHR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF68">
        <property type="entry name" value="GLYCOSIDASE CRH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037299">
        <property type="entry name" value="Glycosidase_CRH1_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0134">Cell wall</keyword>
    <keyword evidence="16" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="12 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="10 18 24" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 22" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133521" description="Crh-like protein" evidence="22">
        <location>
            <begin position="22"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="23">
        <location>
            <begin position="23"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="285"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="291"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="362"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="19">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="19">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="27"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00038074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR037299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR037299-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR037299-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="ODQ57279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="434" mass="46402" checksum="57D488460F442064" modified="2017-01-18" version="1">MVQVKSLWFTSLLVLPAIVSAQSTSACNPLSSSSCAPDKALATSFAEDFKQESKWFSPSSTKDEVHYTDEGLKLTLAKRFDNPSLKSNFYLMYGKLEVILKAAHGTGIVSSFYLQSDDLDEIDLEWIGGDQTQVQTNFFSKGNTSTYDRGTFNQVDSPQTQYHNYTIDWGMEQLVWYIDGQAVRTLKNDTSDGYPQSPMYVMLGIWAGGDPDNAPGTIQWAGGETDYSKTPFSMYVERIVVSDYSSGTEYKYTDQSGDWTSIEAVDGKVNGRMDDAEDEFETLVAGGTIDSSSKTSSGSSSSSESESSSSSEEQSSSSSDSSSSIHDGLSSSRSRVDHIYSSSASSLVTRSESSSNVRNAVSTTSGSAASSATNSTGASGSSEASPTNSSSSTHSVTESSGAGLSLFYPQPIGKSGMKAIIFSVGMTLGIMLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVQ6</accession>
    <name>A0A1E3NVQ6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endoplasmic reticulum-Golgi intermediate compartment protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_81447</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Plays a role in transport between endoplasmic reticulum and Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Golgi apparatus membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ERGIC family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036435.1">
        <property type="nucleotide sequence ID" value="XM_019185236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVQ6"/>
    <dbReference type="STRING" id="683960.A0A1E3NVQ6"/>
    <dbReference type="GeneID" id="30202482"/>
    <dbReference type="OrthoDB" id="5541786at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061852">
        <property type="term" value="C:retrograde transporter complex, Golgi to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045888">
        <property type="entry name" value="Erv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012936">
        <property type="entry name" value="Erv_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039542">
        <property type="entry name" value="Erv_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984:SF81">
        <property type="entry name" value="ER-DERIVED VESICLES PROTEIN ERV41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07970">
        <property type="entry name" value="COPIIcoated_ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13850">
        <property type="entry name" value="ERGIC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter N-terminal" evidence="7">
        <location>
            <begin position="4"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter C-terminal" evidence="6">
        <location>
            <begin position="147"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="340" mass="38860" checksum="544F752AEFF2C63A" modified="2017-01-18" version="1">MANLRSFDAFPKVASTHQVRSTKGSYSTIMLGLFVLFLTYVELGQFFGGEIDHQFKVDNTVQKDLRINMDIVVAMPCNFIHTNVKDLTEDRFLAGELLHYEGFSFFVPEGYRRDDDYDVSTPDLDKVMAEGIVAEFRDRGDAKDSGAPACHIYGSIPVNKVQGDFHITAKGYGYRDRSHVGIESLNFSHVISEFSFGEFYPYIHNPLDATVKLTEEHLQSYQYYLNVVPTQYKKLGVEIDTNQYSTSLLKKQYTLEKKGVPGIFFKYDFEPISLIVEDKRIPFVSFLVRLATIYGGLIIVAKYLYKTFDKVLILLFGKKFAARGEEKKASLLDDYDDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVS8</accession>
    <name>A0A1E3NVS8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_65494</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036443.1">
        <property type="nucleotide sequence ID" value="XM_019183920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVS8"/>
    <dbReference type="GeneID" id="30201166"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="288" mass="33577" checksum="7E80953E109AF07C" modified="2017-01-18" version="1">MSSLCAFKEVLLKRVGDDYSEIFVPFAITKPSDDKTPFFTIYSTKLIDDFPIVFKKVLQLNHNSLPCERLVTVHKSHSSIEDFNKSTIIITQDPQLNLKHLDESFRITEDYFSGVSTFSVGNYKPFIDMQGVEHLKDETIMFGHGENFERNITRNFNDIFKDFEIKEHHFNIFKNLFKNSKTGDSNQSVNVVGMIIVEIPKEKDDDEEKDYMLILKNPSIPIDEFLFEMANYKGSDKAVLLKNDFKLYANDVKVFRIMKRMLHDIFKQLHLPMTRCNRRSSVTTTPEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVW1</accession>
    <name>A0A1E3NVW1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DUF221-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_97964</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036485.1">
        <property type="nucleotide sequence ID" value="XM_019186620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVW1"/>
    <dbReference type="STRING" id="683960.A0A1E3NVW1"/>
    <dbReference type="GeneID" id="30203866"/>
    <dbReference type="OrthoDB" id="1076608at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022257">
        <property type="entry name" value="PHM7_ext"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF20">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN 75"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12621">
        <property type="entry name" value="PHM7_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="322"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="371"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="416"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="536"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="577"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="608"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="10">
        <location>
            <begin position="2"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="11">
        <location>
            <begin position="118"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="8">
        <location>
            <begin position="321"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="10TM putative phosphate transporter extracellular tail" evidence="9">
        <location>
            <begin position="672"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ57278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="751" mass="86397" checksum="5BA697DA683AEE4A" modified="2017-01-18" version="1" fragment="single">YLCFLLVLFTALACINIPVLIPVNYTSGYDHYKPRDLLKYTNGTIPKVTTGLDRISMSNIAPLFSKRLSIHLTMTVISIFLFHGLVIAELLNYVKIKNNYLVTKAQQPPKIPSRNHSNTILIDNVPLNLLDRKLLTKVFSKVSGNKIKNIWFVYNYDELKSKYSKNVHLIDKIEVLETKLISVDENYVDDTTRFGHNKRMFNIRRLGDIRVKFVWYVFPKFYMRNIKETLEVVISEHVENKRKIGQLRASLIAREKSPLNDKKYNKVFIQFKDSFQPHLFSQIQISDKVNALDNTLIFVDPQDLIWENLSVQSNAFVFARVILANTFSVIVILGWVIPVAFIGLISQLPYLTVLIPFLSWLNYLPDYVTDVISNFLSVVLLVCLSETVPFIFRWLSFLKCKKTGAQVELDVQNWMFVFLFIHIFMVVTISSGLTVILESLVNNPVSIPNILATNLPKCSNFFFSYLLIRGLSYFGNNLLQILQLFKSIVTNPFRDTTPRRKFIRLTSIPSYKWGSIYPTFAVLGSIGLIYCILSPLILVFCCLSFVMVLLSFKYSLKYQFNLRNVSENYGQFYPKAIFHLYSGIYFMEICLLGIFILSRDELNQPNCIGHSFITFVLLIFTAAGQIQIQKMFNKLLSKNMPLTLYDPDMNEPEEYELQDLEAGPAAIAKRREEDHNEAPNENLFNDTFHHACFQHTNNLIWIPSDCFSVSEAEINSLKYFGIEGRNRDAEISKNGDLVIQNGPPDGTSVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW89</accession>
    <name>A0A1E3NW89_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_35243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036605.1">
        <property type="nucleotide sequence ID" value="XM_019182287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW89"/>
    <dbReference type="STRING" id="683960.A0A1E3NW89"/>
    <dbReference type="GeneID" id="30199533"/>
    <dbReference type="OrthoDB" id="497541at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021848">
        <property type="entry name" value="HODM_asu-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11927">
        <property type="entry name" value="HODM_asu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="380" mass="44809" checksum="53A023E95344A1B3" modified="2017-01-18" version="1">MCYYYYIIPLLLVLITISFYFRTPKTRKPGVWIPSNYKWPIPEPYPDWNIHKTNPIPYRAFKHKYNVTMGVQSMDPQDWIQLDNEWVKFHDLKQKRVEEKGKDLYDISPIALDGCIELLKELQIYLPNRYPSLFQATSKGLKNLFNNEDYDFKIHDPMFIIGNLLQDDIAILTEDSNGNYNFTAGSILLAGSWRFKDKFNKSISSIHSDSKVPKYHSNLQNSMNKFFYRLKPESPMVRYNYGFQMDDNLPWAYSSLGDEDSNSGWRFQTGEAKALSIDDIYYRSERQSLRRLPKSGVIVFTIRAYFLPLVEICKEPFVPQRLLNGVLNWDEESKEYKGFEKYKDIVIPYLAKQAELQKNQGFTMENEQLNYPLNSKLKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWG8</accession>
    <name>A0A1E3NWG8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_36707</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036124.1">
        <property type="nucleotide sequence ID" value="XM_019182459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWG8"/>
    <dbReference type="STRING" id="683960.A0A1E3NWG8"/>
    <dbReference type="GeneID" id="30199705"/>
    <dbReference type="OrthoDB" id="19141at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02799">
        <property type="entry name" value="tRNA_bind_EMAP-II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF33">
        <property type="entry name" value="AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="4">
        <location>
            <begin position="6"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ56917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="176" mass="19760" checksum="90BED0480D13CF63" modified="2017-01-18" version="1" fragment="single">NQESIKPSVLELKVGQIRECNKHPDADKLYVSKIFTSDAEDATPLTVCSGLVNFVDREELLGRRVVLLTNLKPSKMRGIKSEAMILASENDDASKVEVVHPPVSSAVGELLHFKPFVPEGKPTRLKIKIWEEIQTKLLTDNEGKLIFKDSEQEYRLSGANPDDFAFVDNLRNTIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWU1</accession>
    <name>A0A1E3NWU1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Cytochrome c oxidase assembly protein COX20, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_96785</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the assembly of the cytochrome c oxidase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the COX20 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036687.1">
        <property type="nucleotide sequence ID" value="XM_019186538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWU1"/>
    <dbReference type="STRING" id="683960.A0A1E3NWU1"/>
    <dbReference type="GeneID" id="30203784"/>
    <dbReference type="OrthoDB" id="14603at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043069">
        <property type="term" value="P:negative regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022533">
        <property type="entry name" value="Cox20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31586:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX20, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31586">
        <property type="entry name" value="CYTOCHROME C OXIDASE PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12597">
        <property type="entry name" value="Cox20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007871">
        <property type="entry name" value="Cox20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="141"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="141"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ57480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="177" mass="19761" checksum="8AD328E7779205BE" modified="2017-01-18" version="1">MVWPFNKKENEGDRVVVDESSQSKPRRTSAPRSDQTLLLEDTEPRFNNGPSNPSLPIKEAVDSIKADDFSFNGLVKIPCLREALMTGISSLGVLGTIIFIAQKSPTKAANWGVGGFLLGSTVSWEQCRSKRRKEQAFAAKARETVAAKERPMMHKPEEKITEEVAKSRAEASSWWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWX9</accession>
    <name>A0A1E3NWX9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">3,4-dihydroxy-2-butanone 4-phosphate synthase</fullName>
            <shortName evidence="12">DHBP synthase</shortName>
            <ecNumber evidence="3 12">4.1.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_86011</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18457"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="4.1.99.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="12">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="12">Binds 2 divalent metal cations per subunit. Magnesium or manganese.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 12">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the DHBP synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.12" evidence="3 12"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036284.1">
        <property type="nucleotide sequence ID" value="XM_019185983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWX9"/>
    <dbReference type="STRING" id="683960.A0A1E3NWX9"/>
    <dbReference type="GeneID" id="30203229"/>
    <dbReference type="OrthoDB" id="60371at2759"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.870.10:FF:000002">
        <property type="entry name" value="3,4-dihydroxy-2-butanone 4-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00506">
        <property type="entry name" value="ribB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327:SF18">
        <property type="entry name" value="3,4-DIHYDROXY-2-BUTANONE 4-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327">
        <property type="entry name" value="GTP CYCLOHYDROLASE II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0318">Glutathionylation</keyword>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 12" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0686">Riboflavin biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ57077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="203" mass="22600" checksum="70F3ED5309C6289E" modified="2017-01-18" version="1">MPFTPIPEAIKAFQNGEFLIVMDDESRENEGDLIIAAQFTTQSKMAFLVRYSSGYVCVPLSTDRANQLNLPLMATENNDRHGTAYTITVDYREGTTTGISAHDRSLTARKLADPSVQADEFMKPGHILPLRAVPGLLNERRGHTEAGVHLTKLAGLQPAAVICELVRDQDGLMMRLDDCVEFGKQHDIKIITIEQLVKYIEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWY8</accession>
    <name>A0A1E3NWY8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">A to I editase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_97105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036906.1">
        <property type="nucleotide sequence ID" value="XM_019186559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWY8"/>
    <dbReference type="STRING" id="683960.A0A1E3NWY8"/>
    <dbReference type="GeneID" id="30203805"/>
    <dbReference type="OrthoDB" id="10268011at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043829">
        <property type="term" value="F:tRNA-specific adenosine-37 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002466">
        <property type="entry name" value="A_deamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042935">
        <property type="entry name" value="Tad1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47803">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47803:SF1">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02137">
        <property type="entry name" value="A_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00552">
        <property type="entry name" value="ADEAMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50141">
        <property type="entry name" value="A_DEAMIN_EDITASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="A to I editase" evidence="1">
        <location>
            <begin position="57"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="377" mass="42718" checksum="A442C3F3CB75104A" modified="2017-01-18" version="1">MDDLGTIGDDIAKEVIKQFETNDLKGKPVIRSNGVKEWTVLAGVVALKNGKVINTISIATGVKALPDATIKSHSNGLLLHDCHAEILAFRAFNWFVIQECLKVSNLDRSDYFEFNDGHFNLKDGIEFGMYVSEPPCGDASLELLIDENDEEWRTHTELAGGVLRGRENYTLKGRVRTKPGRRDSPMTLSKSCSDKLAVKQFTSILNGVTSSFVHPKGCYLKYLVIPEDKVDMPAMERCFHDRFNPHPINHMYTLNRLKILTTKETHSFAKEQSNQPPSNTSLIYVPKYNFNESIVNSVKEGYYTKRKSVRKNGESELSRYKLFQESQSLSGGFKARSYFEWKLTNKEYRKAKEIAKEAIGAWCCTATDDFDILKNDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX39</accession>
    <name>A0A1E3NX39_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Letm1 RBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_85815</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036923.1">
        <property type="nucleotide sequence ID" value="XM_019185951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX39"/>
    <dbReference type="STRING" id="683960.A0A1E3NX39"/>
    <dbReference type="GeneID" id="30203197"/>
    <dbReference type="OrthoDB" id="275278at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097177">
        <property type="term" value="F:mitochondrial ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070131">
        <property type="term" value="P:positive regulation of mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033122">
        <property type="entry name" value="LETM1-like_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044202">
        <property type="entry name" value="LETM1/MDM38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009">
        <property type="entry name" value="LEUCINE ZIPPER-EF-HAND CONTAINING TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009:SF1">
        <property type="entry name" value="MITOCHONDRIAL PROTON_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07766">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51758">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Letm1 RBD" evidence="10">
        <location>
            <begin position="158"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="382"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ57716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="436" mass="50498" checksum="7B5F8F201D5C508D" modified="2017-01-18" version="1">MIQLLKNPRIVHGRLVKFPISSIQRSSILYSQRLHKDDPTIPKVPAVDNSPPAAPKPPLLDRIKHEVQHYWDGTKLLGYEIKVSSKLLFRMIRGYELTRREQSQLTRTTIDIFRLVPFSAFIIIPFAELLLPVALKLFPNLLPSTYESKADKNKKKEKLLQTRKSTSSFLRQTFEESGVRLPKAVTEEEKEEFIRFFRTLNTLGETVTKEQVIKIARLFKNDAVLDNLSRPQLVAMAKYMNLRPFGSDQILRYQIRYRLLQIIKDDRALDYEGVESLSLSELHSACVSRGIKTHGVSKARLQDDLKIWLDLRLRQKIPSTLLILSSVYTYGEASNNLDSYYDALVQVLRSIPDQVYDVTKSEVDDDAKLKLNIIKEQEELIKEEKEQEQENKNASTVKDDINLEDYEKEESEKEGNDSKQIEVKWAVWCDSLCKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX73</accession>
    <name>A0A1E3NX73_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Protein ARG5,6, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">WICANDRAFT_85796</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2 18">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">In the C-terminal section; belongs to the NAGSA dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">In the N-terminal section; belongs to the acetylglutamate kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036902.1">
        <property type="nucleotide sequence ID" value="XM_019185948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX73"/>
    <dbReference type="STRING" id="683960.A0A1E3NX73"/>
    <dbReference type="GeneID" id="30203194"/>
    <dbReference type="OrthoDB" id="438291at2759"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00107"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003942">
        <property type="term" value="F:N-acetyl-gamma-glutamyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070401">
        <property type="term" value="F:NADP+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04252">
        <property type="entry name" value="AAK_NAGK-fArgBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23936">
        <property type="entry name" value="AGPR_C_ARG5_6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd24149">
        <property type="entry name" value="AGPR_N_ARG5_6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04263">
        <property type="entry name" value="DUF619-NAGK-FABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000019">
        <property type="entry name" value="Bifunctional acetylglutamate kinase/N-acetyl-gamma-glutamyl-phosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000029">
        <property type="entry name" value="Bifunctional acetylglutamate kinase/N-acetyl-gamma-glutamyl-phosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000011">
        <property type="entry name" value="N-acetyl-gamma-glutamyl-phosphate reductase, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00150">
        <property type="entry name" value="ArgC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023013">
        <property type="entry name" value="AGPR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058924">
        <property type="entry name" value="AGPR_dimerisation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000706">
        <property type="entry name" value="AGPR_type-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041734">
        <property type="entry name" value="NAGK-fArgBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011241">
        <property type="entry name" value="NAGK/NAGSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006855">
        <property type="entry name" value="Vertebrate-like_GNAT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01850">
        <property type="entry name" value="argC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342:SF0">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04768">
        <property type="entry name" value="NAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22698">
        <property type="entry name" value="Semialdhyde_dhC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036440">
        <property type="entry name" value="ARG5-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01224">
        <property type="entry name" value="ARGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51731">
        <property type="entry name" value="GNAT_NAGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 18" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 18" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="11 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="15 18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16 18" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12 18" id="KW-0521">NADP</keyword>
    <keyword evidence="9 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="20">
        <location>
            <begin position="296"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004862"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006830"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00072977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR036440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ57695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="848" mass="93706" checksum="1212C8D1A1550517" modified="2017-01-18" version="1">MGTCNWSIVKVSTVIQLLNNIGSKREVEQYLKYFTSVSSQQFAVIKVGGAIITQHLPELASCLAFLYHVGLYPIVLHGTGPQINELLEAEGVEPDYEDGIRITDPKTMSVVRKCFLEQNLKLVTALEKMGVHARPITAGVFNADYLDEAKYKLVGKINGVNKSPIEASIAAGALPILTSLAETPSGQILNVNADVAAGELARVFEPLKIVYLNEKGGIINGNTGEKISAINLDEEYDDLMKESWVKYGTKLKIKEIKDLLMHLPRSSSVAIINVNDLQKELFTDSGAGTLIRRGYRLLTRSSLSEFSEPELLRKALQRDPEIASGKLSVASYLRELENTNFKSYGDEPLEVLAIVRDGTESTANVPVLDKFLASKAGWLNNVSDNIFHAIRRDYTSLQWVVREDDPNTSWYFSKSDGSYTKNGRILFWFGVDNPESVTELINNFDHRYARIAKSSAESGVFAGQQSRAFSTMRIRQSTPSTTEVRSTRKPIQGFASSQKTFGVRTFATETNPNPPVREGTNTKKSRVALIGARGYTGQNLISMINSHPFLELTHVSSRELQGKKLEGYTKASITYENLQVEDIKRMEERNEVDVWVMALPNGVCKPFVEAIESANGNRSSKIIDLSADYRFDTTGEWVYGLPELNSREKIANARKISNPGCYATAAQIAIKPLVDYIGGVPSIFGISGYSGAGTKPSPKNDVKNLENNLIPYSLTDHIHEREISTQLGTDVAFSPHVAQWFQGITHTINIPLKEPLTSRDIRNIYQDRYRGEQLISVSGEAPLVRDVSGKHGVVVGGFAVNAKQDRVVIVATIDNLLKGAATQCLQNINLVLKYGEYEGIPDDLIIRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXB7</accession>
    <name>A0A1E3NXB7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_35268</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ57783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035974">
        <property type="term" value="C:meiotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071958">
        <property type="term" value="C:new mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071957">
        <property type="term" value="C:old mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902554">
        <property type="term" value="C:serine/threonine protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034973">
        <property type="term" value="C:Sid2-Mob1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903473">
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007096">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MOB_kinase_act_fam"/>
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    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mob1/phocein"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
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        <location>
            <begin position="28"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
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        <location>
            <position position="124"/>
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        <ligand>
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        </ligand>
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        <location>
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        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="270" mass="31119" checksum="559CF2D3D27526BB" modified="2017-01-18" version="1" fragment="single">NGHTIKTTRGFKRTQQGTAASPDTSNSYYPQYQQQQQQPYQQQQQQSAVNNDSNNHNYNSVTSHKDIKHIVESTLGSGVALAQAVKLPRDENINEWLAVHVVDFYNQINMLYGTITEFCSPKTCPRMTATSEYEYLWQDSNLTKPVSVSAPKYVESLMLWIQSLLDDENIFPPMINQPFPPQFQSLVKTIMKRLFRIYAHIYCHHFNEVIELGLNTLLNTSFKHFLLFSDEFNLIANKDFGPLQTLVIEMLVQDDDDNGNNNYENNNEIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXL6</accession>
    <name>A0A1E3NXL6_WICAA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
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        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycotina</taxon>
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            <taxon>Phaffomycetales</taxon>
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            <taxon>Wickerhamomyces</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXL6"/>
    <dbReference type="GeneID" id="30200916"/>
    <dbReference type="Proteomes" id="UP000094112">
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    </dbReference>
    <dbReference type="InterPro" id="IPR049451">
        <property type="entry name" value="AWP2-like_YTTT_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20646">
        <property type="entry name" value="Hpf1_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="134" mass="13077" checksum="B39359F0ED60BF96" modified="2017-01-18" version="1" fragment="single">GSVTTSSGAGSGATSVTWTDSNGETHTGIVTEQPTTGSDGVVGTATTTIPITTSSGAGSGATSVTWTDSNGETHTGIVTEQPTTGSDGVVGTATTTIPITTSSGAGSGATSVTWTDSNGETHTGIVTERANYWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXM3</accession>
    <name>A0A1E3NXM3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RNA polymerase II-associated protein RBA50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_35990</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RPAP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036504.1">
        <property type="nucleotide sequence ID" value="XM_019182382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXM3"/>
    <dbReference type="STRING" id="683960.A0A1E3NXM3"/>
    <dbReference type="GeneID" id="30199628"/>
    <dbReference type="OrthoDB" id="348201at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039913">
        <property type="entry name" value="RPAP1/Rba50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013929">
        <property type="entry name" value="RPAP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013930">
        <property type="entry name" value="RPAP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21483">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21483:SF18">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08620">
        <property type="entry name" value="RPAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08621">
        <property type="entry name" value="RPAP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RPAP1 N-terminal" evidence="4">
        <location>
            <begin position="66"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="RPAP1 C-terminal" evidence="3">
        <location>
            <begin position="283"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="437" mass="49519" checksum="E97E69BE829AD972" modified="2017-01-18" version="1">MDLIGDIVEHDIEAPTPPTFESKAAAEQELSEPKHKDGYQLDGTTGLKRLPKDQQKMNYDNLSEAEKIHLENIEILNNMNEEQRMAEKQELLDSMDPRILKALLLRSEKKLAKKSEAEFEGYGDWIGGDKNGEAWKEPTLDIQDVDEALGIKSSLKTDVPKSPGSAKKVRFDSVSTVQYEDKKEKDEEWEDIEDLDEIAPPSYQINQEADSVPIEEAIASVHFPRPAIDDEETYKLDINDPDFQDKLHEKYFPDLPKDTDKLKWMEPLPELGSGDLVFDNVAELRFDFKGDLIVPTGNNNISTTEGLHHHSENPSLAGYTLRELAHLSRSTVPGQRCIAIRTLGRILHKLGKGKYNIIPEFEPDEDGEEVPINAEALRSAASEFDQMFWGLIDELRVIDTLEESADEKFTPNLSVRNYAIEALWLWKQGGGNKRHAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXN9</accession>
    <name>A0A1E3NXN9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Uroporphyrinogen decarboxylase</fullName>
            <ecNumber evidence="4 14">4.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_33971</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the sequential decarboxylation of four acetate groups of uroporphyrinogen-III (octacarboxyporphyrin) to yield coproporphyrinogen-III (tetracarboxyporphyrin) with the formation of intermediate hepta-, hexa- and penta-carboxylate porphyrinogens in the heme biosynthesis pathway. Acts on a number of porphyrinogens, but only coproporphyrinogen III can ultimately be converted to heme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>uroporphyrinogen I + 4 H(+) = coproporphyrinogen I + 4 CO2</text>
            <dbReference type="Rhea" id="RHEA:31239"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:62626"/>
            <dbReference type="ChEBI" id="CHEBI:62631"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>uroporphyrinogen III + 4 H(+) = coproporphyrinogen III + 4 CO2</text>
            <dbReference type="Rhea" id="RHEA:19865"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57309"/>
            <dbReference type="EC" id="4.1.1.37"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the uroporphyrinogen decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.37" evidence="4 14"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037120.1">
        <property type="nucleotide sequence ID" value="XM_019182136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXN9"/>
    <dbReference type="STRING" id="683960.A0A1E3NXN9"/>
    <dbReference type="GeneID" id="30199382"/>
    <dbReference type="OrthoDB" id="339900at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00321"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004853">
        <property type="term" value="F:uroporphyrinogen decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00717">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.210:FF:000004">
        <property type="entry name" value="Uroporphyrinogen decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00218">
        <property type="entry name" value="URO_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006361">
        <property type="entry name" value="Uroporphyrinogen_deCO2ase_HemE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000257">
        <property type="entry name" value="Uroporphyrinogen_deCOase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01464">
        <property type="entry name" value="hemE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21091">
        <property type="entry name" value="METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21091:SF169">
        <property type="entry name" value="UROPORPHYRINOGEN DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01208">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00906">
        <property type="entry name" value="UROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00907">
        <property type="entry name" value="UROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 14" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="9 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 14" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uroporphyrinogen decarboxylase (URO-D)" evidence="16">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Uroporphyrinogen decarboxylase (URO-D)" evidence="17">
        <location>
            <begin position="147"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ57913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="361" mass="40817" checksum="FC39C5D5B9C4A840" modified="2017-01-18" version="1">MSGFAPLKNDLILRAAKGEKVERPPIWIMRQAGRYLPEYHEVKGGRDFFETCRDAEIASEITIQPIDHFDGLIDAAIIFSDILVIPQAMGMDVQMIDKQGPHFTDPLRTPEDLKKLSKNVDVTKELDWAFKAITLTRKKLNGRVPLLGFCGGPWTLMVYMIEGGGSRLFRFAKEWIYKYPEASHELLQRITDVAIEFLAHQVVAGAQMLQVFESWGGELGPNEFKEFSLPYIRQISAKLPKKLKELGITEEIPITIFSKGSWYALNDLCESGYNTVSLDWLYQPEEAVKVANGRVTLQGNLDPGTIYGSDEIISQKVEAMIKGFGGGKQHYIINFGHGTHPFMDPEKIRHFLKECHRIGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXY2</accession>
    <name>A0A1E3NXY2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Actin-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_80088</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
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        <property type="term" value="P:protein localization to actin cortical patch"/>
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    </dbReference>
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        <property type="entry name" value="DREBRIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3_9"/>
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        <property type="entry name" value="SH3DOMAIN"/>
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    </dbReference>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="ADF-H" evidence="5">
        <location>
            <begin position="5"/>
            <end position="132"/>
        </location>
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    <feature type="domain" description="SH3" evidence="4">
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            <begin position="496"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
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            <begin position="142"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
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            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
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            <begin position="479"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="555" mass="61624" checksum="4EC245E143BE0917" modified="2017-01-18" version="1">MEQINLSTHGKDIQKSYEQVVRGDPSTTWVIYGPDSSKAYKVAEQGTSFEEFLESFDESQIQFGLARVNPPGSDVQKNLLIGWCPDSAPIKSRSSFAQNFAEVSRLLKGYHIQVTARDTDDLDRDELLSRVSAAAGARYSIQSARTNAPSSTSSPAPKAFKPKPAEAPKPAVSKPSPKPEPQVIKPSPASIAKKEAEDEWNEPEIEERDFSKNPLKPNTSTYKPIGRVNLQDVIKEEKSRPDPRLEIEQLKEKERLKKDKELDDYLKTKSATYGAKAPTPSSGLKKDNDKVVGGISKAFGTENGKTPAQLWAERKQKSAGATPEPVSQEEEDKKEVEQVEEEVNVSDLKKKFNNLDVEAEKEEEEESKLPHPSPGLQNAFPKITKLSDKHDFESQKPPAFKNGIPSPGIKSAFPGSDRSEFPGLEKEEEEPKQEEEEEEEEEEAPKPSLPSRGAVPPPPPARNESPAPSLPTRQKEPEPVPEPEPEPEVEEEEQEEAGAIAIAEYDYEASEDNELTFAEGQRIVNIQFVDEDWWLGETEGGDKGLFPANYVTLEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYB7</accession>
    <name>A0A1E3NYB7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pre-mRNA-splicing factor SYF1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Pre-mRNA-splicing factor syf1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_34359</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in pre-mRNA splicing and cell cycle progression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ58199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019182177.1"/>
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    <dbReference type="GeneID" id="30199423"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000349">
        <property type="term" value="P:generation of catalytic spliceosome for first transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Pre-mRNA-splicing factor SYF1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Pre-mRNA-splicing factor syf1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055433">
        <property type="entry name" value="HAT_Syf1-like_N"/>
    </dbReference>
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        <property type="entry name" value="HAT_Syf1_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055430">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF5">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SYF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23231">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23233">
        <property type="entry name" value="HAT_Syf1_CNRKL1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23220">
        <property type="entry name" value="HAT_Syf1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats" evidence="15">
        <location>
            <begin position="20"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor SYF1 central HAT repeats" evidence="13">
        <location>
            <begin position="179"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats" evidence="14">
        <location>
            <begin position="392"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="310"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ58199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="784" mass="91455" checksum="21C376AD6584E7C7" modified="2017-01-18" version="1">MSDSVLKRVNLGLELIQDADLPYEHQILEDPDNLTNWLRYFWHKDSTTEKLFILQRACDHLKRSYKLWIMYLDMRIELVQDLNPIEHKQEFLDVNNEFEKSLYLLNKMPMLWVKYLQFLLLQEDVTFIRRKFTEALRMLPVTQHQKIWPLYLEFADSVGGLTGSKIYLKYLIFNPDDIELVLNRLIQFKDIKNSITVFEKVLNDENFVSKEGKSPLELWLEYADILMSTKKADQNHDTIVENFVRNGIERFPDQQGKLYVKLATYFSKRGAYEKARALLEEGLELVLTVKDFTVIYDSYAEFEESSINKLVELISSKEEAGEDIDELNLELDLQLARFEKLMDKRPFLLSDVKLRQNPNNVEEWLHRISLFNPENLSDILQTYVKAITTIEPFKATEGLSTIWINYSKVYEENNDLSTARTILDKAVKVPFNSPDELVNIWLEWSELELRQDDIEKAIKVLEVATKSPKISKIDYWDSSIAVQGRIHKSIKLWSFYLDLVESSGDIEETCSIYDRVFELKIATPLTVINYANFLEENNRFEEAFKIYERGVNIFKFPVVFEIWNIYLTKALKRNLNIERLRDLFEHALSECPSDLSKPIYLLYSKLEEDNGLIQRSLKLLNKAVDSVKLDDKPEVLNVLIAKTIEHSGLTSTRPILTRAIDELPLKYAIAFVIRFADIEIKLNEFERARELLKYGALQQSPIKNTKLWEFWSDFEVAHGNKETYKAMLRIKRQATEEFNTDILNSSEPVGFVKSSEGPKVSSINASAPTAQANEINPDAIDLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYE1</accession>
    <name>A0A1E3NYE1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDT1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_85725</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GDT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036804.1">
        <property type="nucleotide sequence ID" value="XM_019185938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYE1"/>
    <dbReference type="STRING" id="683960.A0A1E3NYE1"/>
    <dbReference type="GeneID" id="30203184"/>
    <dbReference type="OrthoDB" id="442680at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015085">
        <property type="term" value="F:calcium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032468">
        <property type="term" value="P:Golgi calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032472">
        <property type="term" value="P:Golgi calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001727">
        <property type="entry name" value="GDT1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN HTP-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01169">
        <property type="entry name" value="GDT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="282" mass="30430" checksum="C8AD780D7073B383" modified="2017-01-18" version="1">MSLLKLTENLNIASTDSSKKSTSDIPDEETSKSPLLMAISMIVVSEIGDKTFLISALMAMSHSRVLVFTASFASLAVMTVLSGIVGHALPTLISQRVTQFLASGLFIIFGIKLTKEGLEMSKDLGVDEELAEVEEELEVTNMNHEMSDLEGGAGGANPRQRRPSNPNVEKEAKLINQITESFKSLCGLVLHPVWIQCFVMIFLGEWGDRSQIATIAMAAGSDYWAVISGAVIGHGFCTMAAVIGGKMLASRISMRTVTLGGAFAFFVFAILYFVEALNHTEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYJ4</accession>
    <name>A0A1E3NYJ4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP synthase assembly factor FMC1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_85857</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036979.1">
        <property type="nucleotide sequence ID" value="XM_019185957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYJ4"/>
    <dbReference type="STRING" id="683960.A0A1E3NYJ4"/>
    <dbReference type="GeneID" id="30203203"/>
    <dbReference type="OrthoDB" id="15893at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039196">
        <property type="entry name" value="Fmc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28015">
        <property type="entry name" value="ATP SYNTHASE ASSEMBLY FACTOR FMC1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28015:SF1">
        <property type="entry name" value="ATP SYNTHASE ASSEMBLY FACTOR FMC1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="16474" checksum="77F7DC649D6D0592" modified="2017-01-18" version="1">MTAAATAYKSLYDQLLINTKAAVKKQNAQTLKKTLALLNYQRLNAIKSKEADKLIQINKDIKKANKETEDPQVEVDSILLEGLKVTKETPKNIKHIQDIANFLSYQRTYQELIERYNPGLTMTQEDKVRRTANRVGLDLPEDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYQ0</accession>
    <name>A0A1E3NYQ0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_15973</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037460.1">
        <property type="nucleotide sequence ID" value="XM_019180671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYQ0"/>
    <dbReference type="STRING" id="683960.A0A1E3NYQ0"/>
    <dbReference type="GeneID" id="30197917"/>
    <dbReference type="OrthoDB" id="3364175at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000435">
        <property type="term" value="P:positive regulation of transcription from RNA polymerase II promoter by galactose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14654">
        <property type="entry name" value="ZIP_Gal4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000009">
        <property type="entry name" value="C6 transcription factor (Gal4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005600">
        <property type="entry name" value="Gal4_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03902">
        <property type="entry name" value="Gal4_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="12">
        <location>
            <begin position="7"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="83"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ58253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="603" mass="68529" checksum="075E15F9349E8EF5" modified="2017-01-18" version="1" fragment="single">RNTNSLKCDSCRKRKLKCSKDLPKCTKCVQHNWECTYSPRVVRSPLTRVHLTKVENRVRDLENLVHDLLPDADVDKLLSKQVPKSVTPRTHSEAQSPATQSPLANSVVSVGSSDSNSYHPEVPEDDVPEDAIYGFDWVEEDVESSVNDGMAALSLNPSNKGYFGVASSAVVLRALKIPKEKDEEDSNSTTDSRSNSRGLDFDSTLSLTAKYLTNEFVDSYFANYHTSYPFIHKETFLAQYRDEIPTPTHEIWQILLNTVLALGAWCLHGESSNFDLHYYQNAKSYLSSYVFESGSIPLLQALTLLSNYTQKRNKPNTGWNYLGLAVRMAMGLGLYKEFSNWSSSKNQLQLEMRRRLWWGLYIFDGGAAITFGRPVNLPDEQMMDIENVSNINDDDYSFTGLRQEVTPVSYPTIYSGLIEQVKFTKISTDIYNRLISKPSPNANECLAMNNRIVEFINQLPPFFSENNFVAENASQIPNVSKTPEWLALTRYRLIWRYKNLQIILFRAFVWQRVIKAKNPESSEMTNSPEGKACRRICLECAHETILSVSNFISNHEISVIASWYSTFFLFHAALISVVCLCSEPTSKHSVSWRNDIVLAKKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ05</accession>
    <name>A0A1E3NZ05_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_95005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037139.1">
        <property type="nucleotide sequence ID" value="XM_019186478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ05"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ05"/>
    <dbReference type="GeneID" id="30203724"/>
    <dbReference type="OrthoDB" id="1158011at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133577" description="UBC core domain-containing protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="137" mass="15338" checksum="512B3380F9515F62" modified="2017-01-18" version="1">MSDFHPESWNPAWSVSTILTALVSFMTGNEPATGCCTTTPLVRQNYAKSSHGFNLNNPVFRREFPDLSKDKDKATNELLARNAKAMEEFNKLVGNTPKRKQTVPEKPKTNEVVEVIDDDDDEVVVLDDQSNDPIVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ16</accession>
    <name>A0A1E3NZ16_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribose 1''-phosphate phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_33652</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ADP-alpha-D-ribose 1''-phosphate + H2O = ADP-D-ribose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25029"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="ChEBI" id="CHEBI:58753"/>
            <dbReference type="EC" id="3.1.3.84"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the POA1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.84" evidence="2"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037467.1">
        <property type="nucleotide sequence ID" value="XM_019182099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ16"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ16"/>
    <dbReference type="GeneID" id="30199345"/>
    <dbReference type="OrthoDB" id="2155246at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047407">
        <property type="term" value="F:ADP-ribosyl-[dinitrogen reductase] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140291">
        <property type="term" value="P:peptidyl-glutamate ADP-deribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02901">
        <property type="entry name" value="Macro_Poa1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050892">
        <property type="entry name" value="ADP-ribose_metab_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12521:SF0">
        <property type="entry name" value="ADP-RIBOSE GLYCOHYDROLASE OARD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12521">
        <property type="entry name" value="PROTEIN C6ORF130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="5">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="170" mass="18607" checksum="D108964C5306807E" modified="2017-01-18" version="1">MIQYIKKDLFKAASPSILAHACNTHGMWGGGIALQFSKRFPSTEKIYVNHCSKYSAEELLGTTLLINSSPKDPGNTGTDSTHVIACLFTSEGGGGNADSPKQIVNNTQKAISDLETQLDNAKEDWLVATQKEGDWLVEMPKINAGIFRVPWEDTEAVLKGSKLDYNVYVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ66</accession>
    <name>A0A1E3NZ66_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">inositol phosphorylceramide mannosyltransferase</fullName>
            <ecNumber evidence="8">2.4.1.370</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_34769</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1D-myo-inositol-1-phospho-N-[(R)-2-hydroxy-very-long-chain fatty acyl]-(R)-4-hydroxysphingoid base + GDP-alpha-D-mannose = an alpha-D-mannosyl-(1&lt;-&gt;6)-1D-myo-inositol-1-phospho-N-[(R)-2-hydroxy-very-long-chain fatty acyl]-(R)-4-hydroxysphingoid base + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:64596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:155885"/>
            <dbReference type="ChEBI" id="CHEBI:155926"/>
            <dbReference type="EC" id="2.4.1.370"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:64597"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.370" evidence="8"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037660.1">
        <property type="nucleotide sequence ID" value="XM_019182230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ66"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ66"/>
    <dbReference type="GeneID" id="30199476"/>
    <dbReference type="OrthoDB" id="3647at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031501">
        <property type="term" value="C:mannosyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103064">
        <property type="term" value="F:inositol phosphorylceramide mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006676">
        <property type="term" value="P:mannosyl diphosphorylinositol ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051999">
        <property type="term" value="P:mannosyl-inositol phosphorylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.20:FF:000001">
        <property type="entry name" value="MIPC synthase subunit (SurA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051706">
        <property type="entry name" value="Glycosyltransferase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32385">
        <property type="entry name" value="MANNOSYL PHOSPHORYLINOSITOL CERAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32385:SF20">
        <property type="entry name" value="MANNOSYL PHOSPHORYLINOSITOL CERAMIDE SYNTHASE CSH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="339" mass="40112" checksum="BBFD60CBE6263F8E" modified="2017-01-18" version="1">MRKELQFLISIHLIIVLFLLYQTFDLITLIYDDSYQDALLDIDLNPTSPKDQLIPKIIHQTYKDTNIPLKWQKGQQNCINLHQDYQYILWTDDMAREFISEEYPWFLNTWDNYKYPIERADAIRYFALSHFGGIYIDLDDACEKKLDPLLTVPAFARKTLPTGISNDVMGSVPNHPFFLKAVDNLQRYDWNYLIPYLTIMYSTGPLFLSVIWKQYRRWNTIPESERVRILLPKDYKKHASSFFSITEGNSWHLDDAKFIKSLANHIGLCVFLGFTLAAILLYGEYRLYCWLINGNYKKCFVRKLWNKVIKKNSNIKRNRKNSNLPASEYIQLNSKEVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ74</accession>
    <name>A0A1E3NZ74_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">OPT superfamily oligopeptide transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_85502</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037696.1">
        <property type="nucleotide sequence ID" value="XM_019185903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ74"/>
    <dbReference type="GeneID" id="30203149"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031520">
        <property type="term" value="C:plasma membrane of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034634">
        <property type="term" value="F:glutathione transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098709">
        <property type="term" value="P:glutathione import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00727">
        <property type="entry name" value="ISP4_OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="395"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="451"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="477"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="555"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="598"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="626"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="704"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="766" mass="87815" checksum="102D61B5324FBFD6" modified="2017-01-18" version="1">MGIFGNRSNKDSIVTSSDEVELDGSQYRIDGDVIEIESDNLSFISGDISLMDNSPYPEVREVVPNTDDPSIQINHWRTWVLTTIFVIVFAAVNQFFSLRYPSLTINFLVAQVVAYPIGKFIAKIMPDVKFKYSWFNLNPGPYTIKEHGILTICVALTSSTAYAMNILISQTNFFNRDFGIGYEVLLVWTTQCLGYGLAGLTRRFIVDPPSMIWPQTLISVSMFEALHSSRIDKTIVNGWKISRYYFFLIVMIINFVWYWFPGFIFTGLSYFNFILWGPLTRHNFVANFIFGVVGGIGVIPITFDYTQISQAMSGSVFATPYWAIANTYASVFIFFVLLLPILYFTNTWDAKYLPVISTSTFDNRQKKYNVTRILGDNFVIDKQKYREYSPLFVPFSYLLSYALNFAAVVSIFFHCFLYHGKDIYQKVRDSRAGGQDIHRRLMQNYKEVPDWWYAVLFVIFLALSFVTVCHWDTGFPAWGLVIALLISIVTFIPEGIIEALSNQEVGLNIITELICGYMLPFRPMANLLFKVYGFIVMRQGLNLSRDLKLGLYLKIAPRMLFFIQIYSTLLAGVTQVLVQRWMRFHIKDICSTSQPDGFICASGRTITNAAIIWSIPQYLFTPGQKYSPLLLFFIIGPVFTIGAWLLHKKWPHRWIGKINAPLFFVGPGNIPPSTPYNYSCYFALSFLINKVKNRWPRWDAKYRYVMGAAVETGVAVAVVIVFLCVQYPGGTLKWWGNTVYRDTIDFKGTPFYTLGKGETFGPDKWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZA1</accession>
    <name>A0A1E3NZA1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">argininosuccinate lyase</fullName>
            <ecNumber evidence="4">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_80653</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="4"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037726.1">
        <property type="nucleotide sequence ID" value="XM_019185103.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZA1"/>
    <dbReference type="STRING" id="683960.A0A1E3NZA1"/>
    <dbReference type="GeneID" id="30202349"/>
    <dbReference type="OrthoDB" id="2561043at2759"/>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.10:FF:000002">
        <property type="entry name" value="Argininosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000001">
        <property type="entry name" value="Argininosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000025">
        <property type="entry name" value="Argininosuccinate lyase chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814:SF1">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="6">
        <location>
            <begin position="14"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Argininosuccinate lyase C-terminal" evidence="7">
        <location>
            <begin position="372"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="465" mass="52168" checksum="A5F33BBAC50F80D9" modified="2017-01-18" version="1">MSSDKPAELKLWGGRFTGATDPLMNLYNASLPYDQKMYSADLEGTRVYTAGLNKIGLITDDELKEIHRGLEVIKEEWANGKFVEKDGDEDIHTANERRLGEIIGRQISGKVHTGRSRNDQVATDMRIFVRDELTKILGFLKDLILTIIKRAETEIDVLMPGYTHLQRAQPIRWSHWLSMYATYFTEDYKRLKQIIERLNVSPLGAGALAGHPYGIDREFLASNLGFQGVIGNSLAAVSDRDFVVETMFWSSLFMNHVSRLSEDLIIYSTAEFGFIKLADAYSTGSSLMPQKKNPDSLELLRGKSGRVFGSLAGFLMSIKSIPSTYNKDMQEDKEPLFDCLTTVEHSVLISTGVLSTLSINKEKMEGALTMDMLATDLADYLVRKGVPFRETHHISGECVKKAEDDKLSGIDQLTLEQFKEIDSRFEDDVKDVFSFEVSVERRTATGGTAKSAVNKQLSSLKEQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZA3</accession>
    <name>A0A1E3NZA3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Flavin prenyltransferase PAD1, mitochondrial</fullName>
            <ecNumber evidence="6">2.5.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PAD1</name>
        <name evidence="8" type="ORF">WICANDRAFT_32258</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Flavin prenyltransferase that catalyzes the synthesis of the prenylated FMN cofactor (prenyl-FMN) for the ferulic acid decarboxylase FDC1. The prenyltransferase is metal-independent and links a dimethylallyl moiety from dimethylallyl monophosphate (DMAP) to the flavin N5 and C6 atoms of FMN.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>dimethylallyl phosphate + FMNH2 = prenylated FMNH2 + phosphate</text>
            <dbReference type="Rhea" id="RHEA:37743"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:87467"/>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
            <dbReference type="EC" id="2.5.1.129"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Oligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the UbiX/PAD1 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.129" evidence="6"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037778.1">
        <property type="nucleotide sequence ID" value="XM_019181934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZA3"/>
    <dbReference type="STRING" id="683960.A0A1E3NZA3"/>
    <dbReference type="GeneID" id="30199180"/>
    <dbReference type="OrthoDB" id="5126881at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106141">
        <property type="term" value="F:flavin prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046281">
        <property type="term" value="P:cinnamic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1950:FF:000001">
        <property type="entry name" value="Flavin prenyltransferase UbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01984">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004507">
        <property type="entry name" value="UbiX-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004685">
        <property type="entry name" value="PRK06029.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00421">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43374">
        <property type="entry name" value="FLAVIN PRENYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43374:SF1">
        <property type="entry name" value="FLAVIN PRENYLTRANSFERASE PAD1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 6" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1 6" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Flavoprotein" evidence="7">
        <location>
            <begin position="73"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="81"/>
            <end position="83"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="158"/>
            <end position="161"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="266" mass="30351" checksum="D920C16982B26734" modified="2017-01-18" version="1">MLHPIKRSYGRPCINQAIPKHSLIIPRRHYSINDLERAKAKQQEFEQDEYKEFQEFKKWKEHSARQLAETKPKRIVVAITGATGVAIGVKVLEMLKKLNVETHVIISKWGIGTMKFETDYTLKDIVDLSSFHYSVKDVSAPISSGSFQHDGMIVVPCSMKTLSAIKTGYTEDLIVRAADVTIKEKRKLLLCVRETPLSSIHLENMLSLSNKNVIIFPPVPAFYTRPKTIDHIIQQTSGRILDCFGFDTKEFPRWNGIQNPNVVEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZJ5</accession>
    <name>A0A1E3NZJ5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_80559</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037627.1">
        <property type="nucleotide sequence ID" value="XM_019185084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZJ5"/>
    <dbReference type="STRING" id="683960.A0A1E3NZJ5"/>
    <dbReference type="GeneID" id="30202330"/>
    <dbReference type="OrthoDB" id="3974954at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052942">
        <property type="entry name" value="LPS_cholinephosphotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43404">
        <property type="entry name" value="LIPOPOLYSACCHARIDE CHOLINEPHOSPHOTRANSFERASE LICD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43404:SF1">
        <property type="entry name" value="MNN4P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="1">
        <location>
            <begin position="374"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="633" mass="75064" checksum="E8C22E234E733F01" modified="2017-01-18" version="1">MVSTRDLGLVPYLENLVSIKKASQFFHIKTHCLFNKSDLYLNLNKKFFQSKEFLQHFKIPSTFKESFINDNLAHHEFNQNYSISAYLNHLVDVYSNERDPEAYLKKNQIDFHWGDWVDLAPANPFVKSYDDFLTKFGNNETEMYLFMKKTCYDKLYDVKDSWLDGETFNQKLFVTEHLQSIGICSAIFKYYYATIPEKVVIETDFKYFEMPVNQHRKKDPFGVDGLSKIYYNIDKVSSKQIANFNPNDRVTLVDKLKTTYFDKGFNAEDLKLKPILDQSPDDFKQPDVPELIKKYEAIPNPTREESKYLNFLKYSNAEVGNANKFYFIFPWIQIDSKAELHHYNFPWIKQIISHEERIQVVHHLIRSWFKYAEHAQIISWFNYGNLIGWYFNAQNLPWDNDVDVQVSIKDIDKIGRYHNNTLVVENPKNGDHIFWIQTNPFYLQQNNAQFIDARYIDVKTGIYIDISALWETNIPLPHNFKPKEGEIAIHCKHYNWFSFSDIFPLRRTIYEGAQAYMPNGIEPILKRFYGDKAMNNWNIFDHNWQPDIGLWVPNQVCENAMIPATETRFDEDGQLTLYGACNNTELLHEWKAAKKTHQNHLKEHEAIRNGEDSSKFSEVDFPVFREFNYEEYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P059</accession>
    <name>A0A1E3P059_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RNI-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_63357</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038058.1">
        <property type="nucleotide sequence ID" value="XM_019183532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P059"/>
    <dbReference type="STRING" id="683960.A0A1E3P059"/>
    <dbReference type="GeneID" id="30200778"/>
    <dbReference type="OrthoDB" id="8436363at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF12">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="28"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="114"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="195"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="404"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1186"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="120"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="232"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="262"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="292"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="306"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="407"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ58851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1236" mass="138279" checksum="266FABD6E70EFB41" modified="2017-01-18" version="1">MPDSSQNEIESGVSNVEIDWFLRGKLPSAASCKNPSKSTKLSSKSKAEPDQDSKHTHSPAEDEHNLHSHIKANNGLELKDLLKDEAANEKLHVLSRSKSYSEVDSTSHEKKKTGFLKSLFSRSKNTKQQQSERNSHSNSPEPLNTGNCTGSKNLADGDAKNAISRTRSLSQSNTDLDPKLEEFLRYYKSKGLDRLHQDLEKPSRRASVSSAASTSQRHHHHSKRLVDALGRPIPPHPDVPPLPPAIIYETHFENKSHHQQARPPSTQGLTSSTNSSTAKISGFLRRHNTGLNDSDNVSRNSSFSSQDKRKIEQVMEKSAQESHPVVIPGLEDLPKLKRVAFDVPVFFNDPPQQIPSRTPRKGEVEILKDGSIVIHKLTSAERRQIMNNSGGGIVVGGSGHLKIISNQDKKEESDKAREKTTEDLEEEDNEHEAQKHSIGAAAAAAAAEARAKAAPNELRRIVTNNEDEVMVSSDASKINIDKPMVRHNKSATSLMTMATVHDGQEAEEDENDDIFPPQHVKVPLDILYTRCCHLREILPIPATLKQLKPGSTDPISLLQLRNPKPSLVEVLTFADFVSVAPVLCVALDGVSLSSEMLRIILSALLPKKELEKLTLRNTPIDDLGWRLLCWFLTKNHTLSRLDLTQVPSLTTNVQKPSKTASNIVRMECNMNDRSDMNWNLLNAALISRKGIDELVINGAKMNDEQCMNLFEMGLSISTTRLGLAYNDLTEKQCEIIANHMDVNKIVGVDLGYNDLNGKLGPFNKRVATDDVSESNLKFISLNSTNLDNSNGELDLLISTFAKFKDLRYIDLSNNKKLFPNLIISLSENLPLYPNLARIHLDYNDLKSSEIVAFAELIPFCKSLSYVSLVGNHLDTVSASALTNALKISKSLITLDLNYDEVAPKFRESIGLYTVRNMQHQVYGKQSDPKELQGIQEELSHLLLLDETNPDPKLIEKFFEKAIKIRDRIHETIDELFRLRLQGNLSTDGKETLIRFCFIDASIEKGLSLLDKKTAKHGFDIKKYLTPHPETPLLSRRPSNILHSSSYLSENNHSELLPFGMNTTSHEGDIHIDAEEIKDEQRARAKDTSKIKEEASILKLNQYIKNQMDHKDLKPNIDSIQDISGERFREILLQTNDLNNVVDVLDSIKKEGIPLEKIYERKDSSAGNSTSAPLLLETLKNTHSNVSDRKTIVSDNDDDSDSESDKDSYISDEENDNEINQAYDTILDHLERVRTNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P098</accession>
    <name>A0A1E3P098_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">40S ribosomal protein S17-B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_63385</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038083.1">
        <property type="nucleotide sequence ID" value="XM_019183538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P098"/>
    <dbReference type="STRING" id="683960.A0A1E3P098"/>
    <dbReference type="GeneID" id="30200784"/>
    <dbReference type="OrthoDB" id="1727351at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.60.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.20">
        <property type="entry name" value="Ribosomal protein S17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00511">
        <property type="entry name" value="Ribosomal_eS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_eS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018273">
        <property type="entry name" value="Ribosomal_eS17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036401">
        <property type="entry name" value="Ribosomal_eS17_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116820">
        <property type="entry name" value="Rps17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00712">
        <property type="entry name" value="RIBOSOMAL_S17E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ58876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="17484" checksum="1DBAA06E0A561744" modified="2017-01-18" version="1">MRQGNDRNESRNEGRVRTKTVKRASKVLIERYYPKLTLDFETNKRLASEIAVIPSKRLRNKIAGYTTHLMKRIQKGPVRGISFKLQEEERERKDQYVPEVSALDLSNTKNQLEVDQQTADLVKTLGFKLPLSVVSISSQRAPERRFRKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0G0</accession>
    <name>A0A1E3P0G0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial</fullName>
            <ecNumber evidence="16">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_63257</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="14">Flavinylated by SdhE, about 5% flavinylation occurs in the absence of SdhE.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Mitochondrion inner membrane</location>
            <topology evidence="16">Peripheral membrane protein</topology>
            <orientation evidence="16">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="16"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037957.1">
        <property type="nucleotide sequence ID" value="XM_019183517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0G0"/>
    <dbReference type="STRING" id="683960.A0A1E3P0G0"/>
    <dbReference type="GeneID" id="30200763"/>
    <dbReference type="OrthoDB" id="71672at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045273">
        <property type="term" value="C:respiratory chain complex II (succinate dehydrogenase)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000001">
        <property type="entry name" value="Mitochondrial succinate dehydrogenase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.40:FF:000002">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000026">
        <property type="entry name" value="Succinate dehydrogenase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.100:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase flavoprotein subunit (SdhA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.100">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.80.40">
        <property type="entry name" value="succinate dehydrogenase protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011281">
        <property type="entry name" value="Succ_DH_flav_su_fwd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01816">
        <property type="entry name" value="sdhA_forward"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 FLAVOPROTEIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF51">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000171">
        <property type="entry name" value="SDHA_APRA_LASPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 14 16" id="KW-0274">FAD</keyword>
    <keyword evidence="6 14 16" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 16" id="KW-0813">Transport</keyword>
    <keyword evidence="5 16" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="17">
        <location>
            <begin position="57"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="18">
        <location>
            <begin position="507"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="62"/>
            <end position="67"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="85"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="435"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="451"/>
            <end position="452"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Tele-8alpha-FAD histidine" evidence="15">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000171-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ58750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="642" mass="70373" checksum="23F7A1AE2CC9138D" modified="2017-01-18" version="1">MLSLRQSTKKLFQLNRRAQFSTSIVKRHAYSVNDDISGENAASGVADKYHIIDHEYDCIVVGAGGAGLRAAFGLANAGYKTACISKLFPTRSHTVAAQGGINAALGNMHKDDWHWHMYDTVKGSDWLGDQDAIHYMTREAPQSIIELEHYGVPFSRTEEGRIYQRAFGGQSKEYGKGGQAYRTCAVADRTGHALLHSLYGQALRHNTHFFIEFFAMDLLMDNGECVGVMAYNEEDGTLHRFRAHKTVIATGGYGRAYFSCTSAHTCTGDGYAMVSRAGLPLQDLEFIQFHPSGIYGSGCLITEGARGEGGYLINSEGERFMERYAPHAKDLASRDVVSRAITMEIRAGRGVGPEKDHMFLQLSHIPAPVLKERLPGISETAAIFAGVDVTKEPIPILPTVHYNMGGIPTKWSGEVLTIDEEGKDKVVPGLLACGEAACVSVHGANRLGANSLLDLVVFGRAVSLTIADSLKPGLPHKQLPADLGKESIANLDKLRTATGPKSTASIRLDMQKTMQRDVSVFRTQESLEDGVKGINRVDKSFEQVGTTDRSMIWNSDLVETLELQNLLTCAVQTAESAVVRKESRGAHAREDYPTRDDENWMKHSLSWQKQGGDQTTLAYRPVISTTLDENECKSVPPAIRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0M0</accession>
    <name>A0A1E3P0M0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycoside hydrolase family 5 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_84651</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038188.1">
        <property type="nucleotide sequence ID" value="XM_019185750.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0M0"/>
    <dbReference type="STRING" id="683960.A0A1E3P0M0"/>
    <dbReference type="GeneID" id="30202996"/>
    <dbReference type="OrthoDB" id="9971853at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050295">
        <property type="term" value="F:steryl-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904462">
        <property type="term" value="P:ergosteryl 3-beta-D-glucoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000174">
        <property type="entry name" value="YIR007W-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041036">
        <property type="entry name" value="GH5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052066">
        <property type="entry name" value="Glycosphingolipid_Hydrolases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308:SF5">
        <property type="entry name" value="ERGOSTERYL-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18564">
        <property type="entry name" value="Glyco_hydro_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5 C-terminal" evidence="4">
        <location>
            <begin position="640"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ58981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="745" mass="84125" checksum="A57AFB317AC861AF" modified="2017-01-18" version="1">MSLLKGKIRTHKGSFVDELGRIIQLKGINLDGGSKYPKTPYYPSHKPVKGNEGLFFDGDHVSFVGRPFPLDEAPEHIKRIKSLGYNSIRYIFTWEAIEHEGPGKYDEQFIDYTIEVLKIIDEIGGLYVYLDPHQDVWSRHSGGDGAPMWTLYAAGFDPKTFDKTEAAVLQNYFPHDPKAYPKMLWTTNYKRLVAGSMFTLFFAGKSLAPKAIINGVNIQDYLQDHFIASVGYLVQRIKDAAPKLFEKTIIGVETMNEPNPGYIGTPEIDKIPDNQNLRLGTCPTMFQSVKLGVGIPTEVDEYKISVFGPKKTGTNLIDPDGTIAWLTSDEFDKHYGFKRSSVWKLGECIWAQHGVWDVETTTLLEPDYFATDPSTGAKVDEDYFINNYYIDYYKKFRKAVRDILPDTFIFLQSPPLQIPPRLIGTDLIDSNTVFCPHYYDGMSLMFKTWNKKYNVDTLGIMRGRYSNPVFGIVLGEANIRKSFKNQLLEMSLESQDNLGKDVPVMFTEIGMPFDMDDKKAYNDDDFSSQTSALDALGFALEGNNLSHSWWCYTSDNCHKWGDRFNNEDFSFWSKDDDLDASTLKKLAFQEHSASVTDFDSDTGTLTGSSSALRSQYGTTYSSSSDKPSHEGLRAIDSIIRPYPLALNGTFLDADFDLQQVKYTLKLNGKDVSQSSTRIYLPHWHFPSGDTDIDITSGSVTIDEELEVLEWSHLGGEQTIVIKNLNVKEAEQGWSSCFSSIINSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0P0</accession>
    <name>A0A1E3P0P0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_69392</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038208.1">
        <property type="nucleotide sequence ID" value="XM_019184164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0P0"/>
    <dbReference type="GeneID" id="30201410"/>
    <dbReference type="OrthoDB" id="3981277at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133617" evidence="3">
        <location>
            <begin position="25"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="308" mass="33697" checksum="017B405C0F51746B" modified="2017-01-18" version="1">MVQTSTVAGSARIFQLLFSIVSLALNGKLTTNKSSLSSLVSDLNSNANSSLSILHNTASDGLSLTSSGFTLTSQPFFSLVRFFTTDIEHELPELNNARFGIDKLQTSYLAGISGGEIGSVSLWFGTFVYQTAKYGSWDCSNPENLISQYNLTGDASKQNLNEFLGVSYDGQDESLQNATSDDEINELLGQLWNLTSEPINISRYNASGELTKSELMVKLSQDCYIKKASIGVSFLGFVVFLVSSGAVSYSFMNLYQQLKTKWMSTTSDGEKKADGGEREESDSTPTAPKKYRFGYSYANIFPSVEFDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0S1</accession>
    <name>A0A1E3P0S1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Telomere length regulation protein conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_32613</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TEL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038011.1">
        <property type="nucleotide sequence ID" value="XM_019181986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0S1"/>
    <dbReference type="STRING" id="683960.A0A1E3P0S1"/>
    <dbReference type="GeneID" id="30199232"/>
    <dbReference type="OrthoDB" id="10258062at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.720">
        <property type="entry name" value="Telomere length regulation protein 2, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038528">
        <property type="entry name" value="TEL2_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051970">
        <property type="entry name" value="TEL2_Regulation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019337">
        <property type="entry name" value="Telomere_length_regulation_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15830">
        <property type="entry name" value="TELOMERE LENGTH REGULATION PROTEIN TEL2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15830:SF10">
        <property type="entry name" value="TELOMERE LENGTH REGULATION PROTEIN TEL2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10193">
        <property type="entry name" value="Telomere_reg-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Telomere length regulation protein conserved" evidence="2">
        <location>
            <begin position="415"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="665" mass="77005" checksum="EB94142BEFCCFABC" modified="2017-01-18" version="1">MNDDFSVLKSEPSAEEIDRIVTEFISKENALPTKLQFINTILQHILPIYQSLKTTIRLKIRRAFHSSIGISNLLKFYSLTKNEIYLKFMKDLLLDQTLLVSVIDAAKGNKTELGQVRSLFVGSKIFQTFEGKLEIERYLDLVTKQLIEACKDDKFEVSEFVNQLLSLHPIDAKFVFLNQFFNDNYFNYLVQLYSRMTQVQKKQFLWRSFFPYLDRYVHNNNIDTVTKILKHFVFTDFEDQVVKHIAESKNINLQQAISLLVKDHKTAFKNLLKIWGNEQFIKDHPSELQEANTKELLILINYMDTQTKGNFSKDRTFLDAITSRISSNDNFNRGLGMMVAKKITNGEINFDIVDEIIIEPVSRPNSSSKIDFTDLILGEPLQNLSLNTHKETKVMDSDDESDIEEDQENGNIDTLFLKDLIKKFNDDKISSITKLLAHTTKLVRQKATFKIEIEFYSEELISVLIGLINKFDESNFEDLKLNAIVSVIVVNPKIVSHTMNLLFTGDYSFQQRMIILSSISLAARELRGIDDDFIDKPTYDFPTRKIEQRHQPKIEEVQRAKIEEIKNDSDELIGEGTVKRISKKLTATKEAEKPNNFARLAPKFFYPLANGWIQGINVGTFNEMFMKHYLQTLELVVKAAYPCHEFDDMFALYNEILENSQSILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0X7</accession>
    <name>A0A1E3P0X7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF962-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_84752</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038308.1">
        <property type="nucleotide sequence ID" value="XM_019185771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0X7"/>
    <dbReference type="GeneID" id="30203017"/>
    <dbReference type="OrthoDB" id="2124888at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046521">
        <property type="term" value="P:sphingoid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009305">
        <property type="entry name" value="Mpo1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28026:SF9">
        <property type="entry name" value="2-HYDROXY-PALMITIC ACID DIOXYGENASE MPO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28026">
        <property type="entry name" value="DUF962 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G05310)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06127">
        <property type="entry name" value="Mpo1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="20444" checksum="01A1AC3A0B9217B6" modified="2017-01-18" version="1">MAINLEDQLVFYRSYHFNTINVLIHSVFVPTIMFSSFGYATNVRIPIPIPHLAKYLNLGVLGSLGYSLFYLYLDFIGGALATPILMISSIYFTDLTLYSPEVNKLLIAAFVIGWLVQFIGHGVFEKRAPALLDNLLQALVLAPFFILFELFFLLGFRTDLKKRVDARVEVNIANFRAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P160</accession>
    <name>A0A1E3P160_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_84420</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037859.1">
        <property type="nucleotide sequence ID" value="XM_019185708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P160"/>
    <dbReference type="STRING" id="683960.A0A1E3P160"/>
    <dbReference type="GeneID" id="30202954"/>
    <dbReference type="OrthoDB" id="5368485at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016445">
        <property type="entry name" value="Rog1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF62">
        <property type="entry name" value="LIPASE ROG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005412">
        <property type="entry name" value="UCP005412_abhydr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="3">
        <location>
            <begin position="175"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="645" mass="73310" checksum="1F34DAA465F7ED12" modified="2017-01-18" version="1">MSESDILYRSKRSVKVGQLERYVITYEPEEQEISKLSLKSLWLKVRNIESLPMRAAFLMGPYILYVDVRTEDYHHSKSTFVTADQPKYEPNLSPGQSFNAELSLHNIKKKYVWIVDVVSQILFNASTDVGFELLIGRNLDSLSLKCEKLGSFNSQLTVNRKDTLDLWNLPRPMPNKDIHLVVLTHGLHSNVEADMYYIKEQLDKNAEVTGENLLVKGFPGNTCKTEKGVKYLGSRVAEYIVNELYTEDVKKISFVGHSLGGLVQTFAIAYIEMNFPWFFEKVTPVNFITLAAPLLGIVTDNPAYIKAALSVGIVGKTGQDLGLQVTQGKEPLLKLLPTGPTHKILKKFHNRTVYANSVNDGIVPLYTSALLYLDWKGLTKVANAQRTFINPFQKAMSLLAPNAQAMKSFPKTSMIESAASLLLPPLPPLKYIIDPGSRPNVIIHDRVYTEADIPPKIKRKKTFMQTLDPNTKFEELEEDIAREWHNGMSWRKVLVHLQPDSHNNIVVRRRFANAYGWNVIDHLVENHFTETELDLVNNSEGDELKSLLDRNDIQKDNKEIDLSDMKMSKDTLSPDHEWVNSKNEESFFDAGATGFITNFNEMFENFKNWNMGIGALGVDSTVSDEQNLVDRTTKEDFDPVMENYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P180</accession>
    <name>A0A1E3P180_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_69605</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038446.1">
        <property type="nucleotide sequence ID" value="XM_019184174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P180"/>
    <dbReference type="STRING" id="683960.A0A1E3P180"/>
    <dbReference type="GeneID" id="30201420"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000015">
        <property type="entry name" value="lymphoid-specific helicase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="161"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="554"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="76"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="130"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ59239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="799" mass="92279" checksum="595F5A6751BACFFB" modified="2017-01-18" version="1">MTVEMKVESPIDDAEDAVVNKIIEDGEKKEFRNDQELLDFDQLSTDFKLERLQHLVKQSQVFSNIISDTLLESSLTKKKAREEQKAGGGEPPKKKSKKNGKSSKQANIMDFYKKDNQMSEEAKLTKSKIEQSQTSSTIKQPSLVSGAEMRDYQLEGTEWLITLYENGLNGILADEMGLGKTIQSIALLAFLYEQGIKGSFLITAPLSTVGNWVKEFERFAPSIPVLGYVGSKDERKELREQYFVKNQKAVIITSYEIVLRDFEHLNRISWKFLIVDEGHRLKNVNCKLIRELKKLKTSNRLLITGTPLQNNLNELWSLLNFILPDIFHDLELFQKWFDFSSLNSLSESVKDDQTRKLIDSKIQESLVNNLHTILKPFLLRRLKKDVIKNLPSKREYIVYGTLTSKQSEIYKDALFKSLKKSIMKFALNEHIAVNNLSISKQDIDQFIFKKLDSSYVDFEEDDEEDQESNDRTLRSEKQERLALKLQQNKISKQESQLNDIWTLISKYVDAKKIQNLMMQLRLVCNSPYLFYFPWESEHDMDLAKLLENSSKLQLLDQLVPRLLAKDHKILIFSQFTKMLDLIEDWCELKGFKVSRLDGSVSQQDREVEISSFNEDDEVKIFLLSTRAGGLGLNLTKADSVIIFDSDWNPQVDLQAMDRVHRIGQTKPVVVYRFVIANTIEQVILAKADSKRKLEKLVIQLGKFESLKRLMNKDTQLTFGSGNKKDAIKEENQLAKELAALFEDNRFKTNGHVIEDSKLTEVEIEELLDRSEDAYNRGLDYYKNKGFKHIQIFETTASLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P205</accession>
    <name>A0A1E3P205_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_93649</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038139.1">
        <property type="nucleotide sequence ID" value="XM_019186424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P205"/>
    <dbReference type="GeneID" id="30203670"/>
    <dbReference type="OrthoDB" id="445896at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="1">
        <location>
            <begin position="25"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="100" mass="11730" checksum="AA03F111F5CEFBB7" modified="2017-01-18" version="1">MSEEESTITSDFRASKDLTGSFVEWGLVEIENWLTRLDFHHEVIKKFTLNNITGDLLPYLKDSHFDEMELELKDKIKLKIEINKLLSSQNYLDPNSLEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P249</accession>
    <name>A0A1E3P249_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_63771</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038481.1">
        <property type="nucleotide sequence ID" value="XM_019183608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P249"/>
    <dbReference type="GeneID" id="30200854"/>
    <dbReference type="OrthoDB" id="10596125at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="375" mass="43541" checksum="F6DFDEA7847E1835" modified="2017-01-18" version="1">MTKLDPQVERIELLSSPLILDESTKDEVYQVDDDPIDETKTRTFRSQSPLRFRERLAGLFGGGGGGRATVDEQLKSIADAEDQQLNMDPIGEEHETIKSTETRGSSLINEQQTYYNTIRNQYISDVEPLSDPSIENKVPINDVPPSRLFLSIYPDQVDYFKRDKDMTLDKIKNYDLMYKWNLWSDKNISNVSRPGTSASNVTFLDNDQAKTSEKRRLKKEFPICSKIKLNETILQYKKSFKQSNETFHIHIFKDGIKPKRNIKEFENGGILAMQSSDCHYTHWKSLLRIFLDDKFHGEEYPDVYALKDKVCGLSFIMKEQHILLTKLWLKDVDKEKVSQLCRLISENIHEPLLLHALDNAKFMNNKSQTIEKVFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2H8</accession>
    <name>A0A1E3P2H8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">low-specificity L-threonine aldolase</fullName>
            <ecNumber evidence="8">4.1.2.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_29852</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-allo-threonine = acetaldehyde + glycine</text>
            <dbReference type="Rhea" id="RHEA:26209"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58585"/>
            <dbReference type="EC" id="4.1.2.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-threonine = acetaldehyde + glycine</text>
            <dbReference type="Rhea" id="RHEA:19625"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.1.2.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid degradation; L-threonine degradation via aldolase pathway; acetaldehyde and glycine from L-threonine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.48" evidence="8"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038760.1">
        <property type="nucleotide sequence ID" value="XM_019181659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2H8"/>
    <dbReference type="STRING" id="683960.A0A1E3P2H8"/>
    <dbReference type="GeneID" id="30198905"/>
    <dbReference type="OrthoDB" id="10261951at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008732">
        <property type="term" value="F:L-allo-threonine aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006545">
        <property type="term" value="P:glycine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06502">
        <property type="entry name" value="TA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000030">
        <property type="entry name" value="Low-specificity L-threonine aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001597">
        <property type="entry name" value="ArAA_b-elim_lyase/Thr_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023603">
        <property type="entry name" value="Low_specificity_L-TA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041359">
        <property type="entry name" value="GntG_guanitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097:SF9">
        <property type="entry name" value="L-THREONINE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097">
        <property type="entry name" value="L-THREONINE ALDOLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01212">
        <property type="entry name" value="Beta_elim_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017617">
        <property type="entry name" value="Thr_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aromatic amino acid beta-eliminating lyase/threonine aldolase" evidence="10">
        <location>
            <begin position="10"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00050410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR017617-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="366" mass="40432" checksum="1FC2AA90733B9C3D" modified="2017-01-18" version="1">MQPYKSAKNDFRSDTFTTPTESMVKAALTASIGDSVYNEDVDTIALELKVAKLAGKPAGLYCVSGTLSNQIAIRTHLFQPPYSILCDYRAHVYTHEAAGLAMLSNAMVTPVRPANGDYLTLEDIKQNIVPEDGDIHGAPTKLISMENTLHGIITPVEELLRIKQFCVENGYKLHCDGARIWNASVEANVPLSKYGEIFDSISICLSKSIGAPMGSVLVGEKPFIKKANHFKKQCGGGIRQSGMMAKMAMVAIDETLPLLKSSHEKAKKLGDFCIENGIILESPVDTNFVFIDLKANHIKDDDLIKYGKIHGVQLMGGRIAFHYQIDDEALENVKKAVYDAHQNSKKNPYYHDGPYKMYRSPTPVKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2I3</accession>
    <name>A0A1E3P2I3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_91328</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038812.1">
        <property type="nucleotide sequence ID" value="XM_019186288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2I3"/>
    <dbReference type="GeneID" id="30203534"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="133" mass="15241" checksum="02BFEC97EA775939" modified="2017-01-18" version="1">MEIAKRVPNKITSLAIDGWDSIQHIDAPSFGDSTHMLVGKSHRRLIKNLFASPLTKSTSFSLEDIHLFDQDLDLEKLDFPNVSSVKLHNCGAINKFSKFKRPCVKYIQVFCEDRHVSILNSLAFEEYNFLKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2R5</accession>
    <name>A0A1E3P2R5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_68359</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038983.1">
        <property type="nucleotide sequence ID" value="XM_019184115.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P2R5"/>
    <dbReference type="GeneID" id="30201361"/>
    <dbReference type="OrthoDB" id="2123378at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000142">
        <property type="term" value="C:cellular bud neck contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007120">
        <property type="term" value="P:axial cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097271">
        <property type="term" value="P:protein localization to bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13278">
        <property type="entry name" value="PH_Bud4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052007">
        <property type="entry name" value="Bud4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36100">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36100:SF1">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="1458"/>
            <end position="1564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="69"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="162"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="465"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="527"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="577"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="706"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="785"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="954"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1071"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1166"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="162"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="207"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="246"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="301"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="465"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="527"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="608"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="785"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1184"/>
            <end position="1202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ59776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1580" mass="178008" checksum="20A56B10376F0DD9" modified="2017-01-18" version="1">MSISMDLNRYSTPIKKEFMDSDDESIDESQPMIKKEYQNHSVDDLLHAIDKNISSTSFHNENSFLNTSNTNSNSFNSEASNDPSPIKPLNWKQQQQENHQSLNNLLGELPNLKTPLKENVMQDIPRAPSFDELEFNTKVNRTSSLSRLSGPRQLSSSSICRTFSNKLPNSHSNKSLGGPSTRDNSPSKKSVCFENSPPKIMEYDELTPEHSDESFDEDRKDTNPFESNWNANVKPSLHALPPLPPKHTISSSISSPTKETSIGSVDGSFEDELTHEELQKDKSSLTLEKRLDLVLGSNEVSERVQNDEMTKGSSSPQRSLTKKEEDLIYNIKSKEEIEREQSLRDAADLFEVDLRNNDKESNDLSLAPQLKRRGSNNSLRDEERELQTIVVQNTSAPIVLEDGMKNVDVASLNRSTDDLLSLQSAFTNNDTESFASATEIYDNESEDNDEPVDTAEQSIMRMLSENSANTNNGFQSSTSISTLRNGSSSDSPVKTHKKKLSLSDSINNGLSYISSFGKNMKDAIHKRTDSGSEIKQEDPQSSFSDYGDGNEEAPSEAPLEAHPVPVVEIIDTHDVQSYASSVTEHDYETPRNSVEPSESLPLLDHQTAEPSPSFTPDESQDNESAIDMLDLQPPLKLTNETDEADGSFSDEFKETPLFNTSDTSDSKNVLPFGIHIKQETQVDDEVDNEIPTGELDEVSLNQIETSLSENSSEILKFESEPEPEMASSFKQEFKEEEEDVKPEIPVIKHELPSFNSTLFHDKEPLDDDDVLELQTPPTDYISIWHSQPKSCSPKPQYSVSQRKVSPPEKVLKLLNRRISSSGDSDGEASKRNSVLSIGSSSIDLSRRNSLITTSVSKPSYIRVGSRNSFLHDLSKDHNDSSFINRSLKGPDVSVVDSSNVLANSSDIVLPELTNSSDFANAFKEWEVEKQLDNNNILKDLEQDTEQLSAAKKLKDTTNHQDIKNIWQNGSNDPTPTPPPAATGEQKSRTPSIDTGKIKNFIAQNGVQGEFANVKSSEAVGRVIVSNNEDHSIELEYDQDSRYNSLNNSKIEPADKFVDAEEHQAVLPVIQQEPEPQPEEEPRHKRFISGSSTNLDNLYDLNEEFDKALQIKEHGYATREPKQVIIAKSQTNNKDNDMLDPVELAKPLVPEADNINYVKQREQKVKSKYGDRVPSARVRAPLSNVGDSNLNAQSNKPKSRVPSANTRVISDEYKPGELLMKKREPHVTKRVSVAPNENVDLPKSDLGRLYIHINTLDDVLLDQIKYHKASFKLYLDNGKHVISTPKATLDNNQKIDKEFEVAIEEDSTDLFFTLKVSYHTPDQELVEVVEKTPIKSNRLSRLLGFKQKFKYEKKFITQKKTKDDWDNKFANDGSFGKNKISFSAQDNDVSGKCQSYIVELFNEWEVVKQSDKIYKKPAHLVGKLNVDMLFLPRTSPLETLPPSIKIGYKIADHLVEQSKINYEGYLFQEGGDCDLWKRRFFKIEGTKLIAHQETSKKPRAQINLLKVVDIIHDGKKTNSQRNFTDEILMSDCFKLKFLNGEIINFNAETKELKDEWIQNLEKVVELNKFHQPWVKLLTQNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P304</accession>
    <name>A0A1E3P304_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CP-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_62172</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038791.1">
        <property type="nucleotide sequence ID" value="XM_019183320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P304"/>
    <dbReference type="STRING" id="683960.A0A1E3P304"/>
    <dbReference type="GeneID" id="30200566"/>
    <dbReference type="OrthoDB" id="61815at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01857">
        <property type="entry name" value="HSR1_MMR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001558">
        <property type="entry name" value="Ribosome biogenesis GTPase Lsg1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043358">
        <property type="entry name" value="GNL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709:SF2">
        <property type="entry name" value="LARGE SUBUNIT GTPASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709">
        <property type="entry name" value="LARGE SUBUNIT GTPASE 1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="7">
        <location>
            <begin position="190"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="598"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="610"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="630" mass="71838" checksum="3BC09579086C05DC" modified="2017-01-18" version="1">MADSGKRKMGFHEAPKKFKIPKGPKPVHHKNKNTIGLGRTIANVRAKENKVEYLPDGEMRFTTDKHEANWVKLRSVTQENALDEFLNTAELADTDFTADKNNQVTIIKVDPTKNPYLLTNDQEKRVSEIQHANENKLTVPRRPEWDESMTRFQLDRQEKEAFLDWRRELARLQENEDLLLTPFERNLEVWRQLWRVVERSDLIVQIVDARNPLMFRSPDLEKYVKELNPLKKNLLLVNKADLLTPKQRRSWAAYFKSHNINFTFFSAFEANQLLEKQDQPDYKPELIEKVEDQKDLDESTRILKIEELEELFMTEAPEPLTTPPNGQPPILQIGLVGYPNVGKSSTINALIGSKKVSVSSTPGKTKHFQTIHLSDQVLLCDCPGLVFPNFAYTNGELVCCGVLPIDQLREYTGPTQLVAQRVPKFFLEAVYGIAIPTKSKDDGGDGVPTSQELLTAYARARGYMTQGFGSADEPRSARYILKDYVNGKLPYVHPPPNPDGSPLTQDEKRDFNKELYTIDSLTENRKEQILSAIHSKGLKDFNLADDLSKLSFSSHIGGDDKKESRSANYGGKQAAIYNATEDLDREFFKMNGVQGKFSTPFHKVENNTGNKKHNKKNKSKNQKVKKADDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P348</accession>
    <name>A0A1E3P348_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_83810</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038945.1">
        <property type="nucleotide sequence ID" value="XM_019185601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P348"/>
    <dbReference type="STRING" id="683960.A0A1E3P348"/>
    <dbReference type="GeneID" id="30202847"/>
    <dbReference type="OrthoDB" id="446890at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047066">
        <property type="term" value="F:phospholipid-hydroperoxide glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140824">
        <property type="term" value="F:thioredoxin-dependent peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00340">
        <property type="entry name" value="GSH_Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029759">
        <property type="entry name" value="GPX_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029760">
        <property type="entry name" value="GPX_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592:SF78">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000303">
        <property type="entry name" value="Glutathion_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01011">
        <property type="entry name" value="GLUTPROXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00460">
        <property type="entry name" value="GLUTATHIONE_PEROXID_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00763">
        <property type="entry name" value="GLUTATHIONE_PEROXID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 8" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="7">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000303-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="158" mass="18678" checksum="21F060C2954F6903" modified="2017-01-18" version="1">MSPIMQQSTDVVNFHDLYTFDCDDNPIYFHSFIGKIVLVANVATQCASAYQLRQLEALYQKYKDRDFVVLAFPSNQFHQEKRGTNQEVADNCRKRFDVSFPIMEKVKVNGKNQSEVFRFLKENKHGFLNTKRVKWNFEKFLVDRDGTILKRYSTQRFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P362</accession>
    <name>A0A1E3P362_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine-protein kinase MEC1</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ATR homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">DNA-damage checkpoint kinase MEC1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Mitosis entry checkpoint protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">WICANDRAFT_63827</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PI3/PI4-kinase family. ATM subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038539.1">
        <property type="nucleotide sequence ID" value="XM_019183620.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P362"/>
    <dbReference type="GeneID" id="30200866"/>
    <dbReference type="OrthoDB" id="381190at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00892">
        <property type="entry name" value="PIKKc_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000033">
        <property type="entry name" value="Serine/threonine-protein kinase MEC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056802">
        <property type="entry name" value="ATR-like_M-HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057564">
        <property type="entry name" value="HEAT_ATR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058681">
        <property type="entry name" value="HEAT_MEC1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012993">
        <property type="entry name" value="UME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF125">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE MEC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23593">
        <property type="entry name" value="HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25385">
        <property type="entry name" value="HEAT_MEC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25030">
        <property type="entry name" value="M-HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08064">
        <property type="entry name" value="UME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00802">
        <property type="entry name" value="UME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="21">
        <location>
            <begin position="1291"/>
            <end position="1844"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="20">
        <location>
            <begin position="1954"/>
            <end position="2268"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="22">
        <location>
            <begin position="2252"/>
            <end position="2284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="ODQ59332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="2284" mass="260889" checksum="B621878510B77ADA" modified="2017-01-18" version="1">MSSGTTIAVDALNELVDTVLSGGSEDDSSETQLKLLTIIIKHGIQSKHRPKGTSQTEWVGKCLSAIELICEREPLLLNRESEDENVVIYKWILNSLIPLLATNDFVLVNNVKQTVITMIHIITAKLRLFSYKKDIRTFFLTTFDSYLTSLFKTSIEEKLINILSISTNLLSLLTDQELRAKMMIESFYDQTFESSARKLDYIISGLQNSIKNDKIDAIRSHLISSILNYTINGEYIMYPRLKLFLGLCYETIHSPTLPSHPSTQYTISYALLKTLSKCLDTNNQDLFKNSLSLKNTKEVVDRVSSFSSSFVKTFELLEYALALANGQDCGTSTTTAFEDKSLETIRLSIISKLVKKSDTLIMMNNLRLEIPPDCNTLTKWINSAKKSLEQPPEKYKAAPKVFYYNIITSLGDFFCMNDNFYNGKTGLCSVCCKTTLNLQHIKEDRSPIEKNKDVQLAYLLLSRYFLPDELVNGDTVLSMATLLTIYKIFAHYQPPKADSNCEFYKFVASHIQSQNRDIRLLAARIMPLYFISKHNIDDSNSEIIFKLLNSINMHENLYLAESTIMIWGQLAIASEGERLNAILFKLIDFLGSQNSFQSSIAFHELQIIAAAKDKTPWQLLAPFMSNLSLTIAKQLLTKPTMGQRVSELVGVSVSTILARTETQTVPYLLTYYKNDLISEIAKATGKKKIELVLDNRAKIFAVLLTSVVDVTEGKLHSILSNVCPELKNLALADLIVLIPTVWELLKMYSIELKNQEKIKKAIAFIFNITSTGESTSAALAEFFQRHILGVVQQFSDAIHDTKGLQPYVEKVKALNAIELLVQIAGSSIVSALPQISTCLQAALEIDLLRESCLRCWRILVQHLEESHLVTILDLSLAIVLQRWESFNSKCRKEAKTLLSAFFDKSELFRTKHVHCFFSLSDNDELLDLYTKVHQIIRKNERPNSLLYDISRRCKNDNKFVVKQALMDLNRFLVQYQTDFYTTFLPRQSYAAVVSTLYAVLLDILHKFKSNNDITIQCSKCLGLIGALDPTKYEIEKKRDQLIIASDFEDPKETVRFLLKFIDQNLVPAFWASEDPGKQLFLAYAMQEFLKLCGLDSSRIKVDSLDKSSHEYLLWSQFTDISKSTLTPLVSSKYSASVSNYDPLKYPIYKVTKEHSQWLRQFTLDLLKKAKGNNAEPIFFVCSSLVKDQDLSFCTFILPYVALNLTISQTNGHEAENIKKEIMAVLSVSLKEVHHLAADSLRLCYESIFNLLDYFRKWIFARKQLLKRRNIKKSDPAIKAVSDLLDSIPQQLMAMRSLQSNSYERAILYMEQCYRTDSYQDYFGDSFFTTMQNMYANIGDIDALDGVLKNFSTKSLNDRITELEYSDKWKMAQDCFEALGSSNGEYVDIDANTRLLNSFYNHNLYDRTLQKLDSLIITSQPLKKDWLNIGMETSMLSGNLESLLKWVRLFESSEEINDSQLLVNYHIGKALLYWKDQKMDMFESSLENAHMLIGGRLSMNRPVTLNKSRPMLVVLHSLSDITAISHQSSTHTFNSLASLMDLRLENTGNDFMANWYISSLRKVADFLAGNSFNLTDLSDTWVSAAKIARKHNRLDLSSSAIMNAIRLGNSNTDLEYAKLLWAQGDHSRALKFMEDLKNEERPLSERGKAEIQLKYTKWIDYSNNSSSTKIIEEYSATIKLDSTWEKGYYALGKYYNKLLDLKTSPTSEKINPRDISGEYERKTISFYLKAMQCGIKYLYEALPKVVTVWLDFSANISEVPGELQDTTKVTVLAERRKHLQSIHDDIAKASEKLPKYIWYTVFSQLLSRILHPNKVTSQLIIMIANQVVLEYPEHALWSLLAQYKSTQAERSRKAKKIIDLFTSSESKNSSISNATTVQAAKKIFNELIEVSKKQLPKVKGPLSLKDDFGFDHTSVPCPLVVPAKINFDITLPTSVQALKYHNPFPSTSRVTINRFDNRVDVLSSMQQPRHLYLRGSNGQTYSILCKPNDDLRKDAKLMEFTTMVDHLLRKDYESEQRKLHIKTYAVIPLNENYGIIEWVENSRTMRDILKVHYSNVNLGLDIATIRKVLDKDCDVSEKAVLFKKDILDQYPPVLYHWFIENFPDPSSWYDARNNYTRTTAVMSMVGYMLGLGDRHGENLLFNEKDGGILHVDFDCLFEKGLELTVPERVPFRLTRNMVDAFGITGVEGTFRKSCEVTLTLLRGNETTLMNILESFLHDPIMDWSHKRKTRNTPQSALSTIRRKIRGILDKEGLPMSIQGQAEFLIQQATSLENLCQMYIGWMAFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3A8</accession>
    <name>A0A1E3P3A8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_53559</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPS family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039166.1">
        <property type="nucleotide sequence ID" value="XM_019182714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3A8"/>
    <dbReference type="STRING" id="683960.A0A1E3P3A8"/>
    <dbReference type="GeneID" id="30199960"/>
    <dbReference type="OrthoDB" id="295029at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007587">
        <property type="entry name" value="SAPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634">
        <property type="entry name" value="SIT4 YEAST -ASSOCIATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634:SF14">
        <property type="entry name" value="SIT4-ASSOCIATING PROTEIN SAP155-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04499">
        <property type="entry name" value="SAPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="30"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="126"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="772"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="64"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="126"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="794"/>
            <end position="809"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="809" mass="92002" checksum="264A5A56BF7DB09C" modified="2017-01-18" version="1">MSFWPFGQNANSASHLNRLLDEYYYERSNASQQQSSTNAQPSEVDPSFASASTTHNAATSSNNQDTSAIAGSSSNVTPGLDQEKENIKIDKEFVYDLLDDPSFLNELSRQNNKLIDFICFGYIDKEDDEDEGGDESGVDQDNDVDMNDDDNDNTKQQKQQTQSTQGKSILEVLLDLVVDSEQWFEENEQQLYQASENQLDEDETTANDITTTFNRIHTASEVLSCKIWLISENLVEEPQLLNKLWWFLKNDFKSSSPSIQFFIKINEQLLESRPDQMLNFIRSLQDLVDLFLKHVNITMIMDFLLRIITTDKADNPTGIIELLSEQKLIPKLLNLLYPDHDSSIQSSCGDFLKALIAISANTSIDENTIGPNLLTKELVSDTCIDKIIEIMLLKGNGLATVVGVIIEIIRKNNSDYDSVNLLYTTTESNPPNKRDPIYLGNMLKKFAQKLGSFNDILTDKELTSKRIETQINQEIEPLGFERFKICELVAELLHCSNIALLNNHLTEKIIEERENFLEQQEFNLKNALSEDLINSNNTKHSASPSPGANSYNGSTFSNLNIDVKSQEDGSSGSHHHQLPQLHIPVVTKKEQLRSNPTVGDVFKIALIDSNVLTTIIDMFVKFPWNNFWHNVVFDIVQQIFNGRLDISYNPYLILELFDKCDITNLIINAYKLCIDTERETNVRLGYMGHLILIAEEVVKFSSTFQNSKFNDTEADELIYTKLIDENWISYVTNVLTETREMYNCVLGGIKANEFSEGDYINHNTIVLGNSEEEILNQQTEDGGPPDEMFYDSKETEEEEEEEEEDKEMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3G7</accession>
    <name>A0A1E3P3G7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_78524</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039100.1">
        <property type="nucleotide sequence ID" value="XM_019184740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3G7"/>
    <dbReference type="GeneID" id="30201986"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="195" mass="22455" checksum="84461DBDD085312C" modified="2017-01-18" version="1">MSDSHYQTAHHVQTHSPNFNHSQLQNTNNIKDNSISNHHHQQPEENISYSPKIRDVANLNNNTITFLAKPNSNASDSSNLIKIGKCFVTDAGARKVLTKSIDSDVEKLARSNVQTLQTLPTPFLFLFLLFSTYGSPTSMPDFRSNKIYHGLFCYWFHRILIGDNLFPLKIGFGLDHWYIVFIRPFCYYWESFPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4G3</accession>
    <name>A0A1E3P4G3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_15169</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039301.1">
        <property type="nucleotide sequence ID" value="XM_019180635.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P4G3"/>
    <dbReference type="GeneID" id="30197881"/>
    <dbReference type="OrthoDB" id="3981091at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032065">
        <property type="term" value="P:maintenance of protein location in cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13365">
        <property type="entry name" value="PH_PLC_plant-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053005">
        <property type="entry name" value="Nuclear_Pos-Cytoskel_Interact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024774">
        <property type="entry name" value="PH_dom-Mcp5-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28190">
        <property type="entry name" value="NUCLEAR MIGRATION PROTEIN NUM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28190:SF1">
        <property type="entry name" value="NUCLEAR MIGRATION PROTEIN NUM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12814">
        <property type="entry name" value="Mcp5_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="1300"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1242"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1242"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1425" mass="159067" checksum="048E43B0998E918D" modified="2017-01-18" version="1" fragment="single">LSSELKTLTEEDVRTYADQHDLVLIPSDEYNKLSKRVDNPTHEYLSEKSSKQGLTVLPKLAHVSLLDRANKSIHDLAKEENAVVLKEDEHRELTSKAHEPTLEHVTTKAQLHKHKVIPTEEYDDLFKKANTPIEEAATAKGLVLMSNDDHADIQSRLQSPTLDSLHSHLQSHQHVAIPQDDHKSLVEKANKSIETQAKEKNLVLLPKDDHDELVRKAEQPSLDELKEFAASQSHVVITNADHEALVTHSKKSIKELAEEENHVVLSKDDHAELHRKANYPTVDELVAAAGHKIVKIDEHDDLVERANKTIDEHAKELSLNVLSEAELDALVNPSEEALSKNAKKLGLVAITKADHEKLVTESKAPTLEHITEKAKVLGYDVVDSNDYQSLVKSSESPSKEFVRAKAATLGLIAVPEVDYSNLKEEAAKLKKDFADKSLLVEKVSSDGSVVLKKEDYDTLVNKANTSIEDLASSEGKVVLTKEDYDVLLEPSKDSVIDHAAKHDLVAIPKDAHHTLLRNHESPSLDHLSSKASVHGHVVVPKDHYDEIKGLVESPSSSFLSEKARDINSILIPVDVHEALKVKANRTIDDLAKQDNKVVIDEHDYESLQNPSIEKIKDHADRAGLVATAKDDHEETQKTLNEPPLTFLQDKAKSHFHVLVHQDNLKDLEDHKENPSTDFLSTKAASQGMVVVSKAEFDRLKEFSQKSVETIASEKNLVVLPSDEFKALENPSIEILNTKATDAGMVLIKAKDYEQLVHPSKDDLTKSAAEKDLTILSNKSFEDLKLKSEKTIESLAEESGKIVLTSEEHKKLEHPSKERIVSLASEMTMVALPQEDYQKLIHPSAEFLKEHSDNLGLVLVPKEELLAIKEKSSKTIEDLAKESNSVVLTKDQHSALKNPSQESIVEHAAKFGLITLSTDNHSTLLHPSKEVLSVHADREGLKLVPIEEFKTLANPTKDIIKEKALENGLVAIEKSEYDDLHSSAHSPTADHITQKAAAIGYTVISSSEHEHLSRSVSNPTREFIESGATKHHNMSKPDLEAKAEKINHVVIPKEEYEEYVRVDQERSLDEPSTPVSKVAANKDYFENIHKSAEKESQKEEIDILRNKAADRNKEELDKPKAQPPVLSREEIEAGARVHGLLAIPENSFIATNISRIPDVNNVVVLPVTYYNKLTKSEQLNLDKISNDELQAQARKRGFHVTYGEVDFNRDIPPLTRKNTITSLTSSNSRRNLAEAAASAAYQEYENQPKTMSRSSSRARSISRSGSAANIHQRDVSIDGGISLMTDASLSEPNIIPALTQTVIGEYLYKYYRRLGPLSSISESRHERYFWIHPYTLTLYWSSTNPVLGNPSTHKTRAAAIIGVESVDDNNPLPAGLYHKSIIVHSQTRSIKITCPTRQRHNIWYNSLRYLIQRSMDGINLEEDLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4G4</accession>
    <name>A0A1E3P4G4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Phosphomannomutase</fullName>
            <ecNumber evidence="5 13">5.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_29993</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>alpha-D-mannose 1-phosphate = D-mannose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11140"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="EC" id="5.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the eukaryotic PMM family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.8" evidence="5 13"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039367.1">
        <property type="nucleotide sequence ID" value="XM_019181676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4G4"/>
    <dbReference type="STRING" id="683960.A0A1E3P4G4"/>
    <dbReference type="GeneID" id="30198922"/>
    <dbReference type="OrthoDB" id="10264771at2759"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00424"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02585">
        <property type="entry name" value="HAD_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1240.20:FF:000001">
        <property type="entry name" value="Phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005002">
        <property type="entry name" value="PMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043169">
        <property type="entry name" value="PMM_cap"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466:SF0">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03332">
        <property type="entry name" value="PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ60160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="252" mass="28690" checksum="CECDA2F1DAD0C20D" modified="2017-01-18" version="1">MTSFQDREFPNALILFDADNCLTLPEKPVCEELKMLLCQLRIKVAIGFVSGSDLSVQVEHLGPTILKDFDFCFSENGLVAYKLGQLISSQSFISWIGDENYNELVSFILSYLAHLDLPKRRGTFIEFSNGMIKVSPIGKNSTIEERIEFERHDKEFLIRESFIKALQAKFQSLGLSYLISGSISFDIFPSGWDKSYALRHLENEGFDEVYFFGDKCIKCDNSWTFGDSGIIHCPVQGPEDTFIKLRDIFQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4Q0</accession>
    <name>A0A1E3P4Q0_WICAA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">WICANDRAFT_78937</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="30202054"/>
    <dbReference type="Proteomes" id="UP000094112">
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="194" mass="22353" checksum="D3B804FDF55427C0" modified="2017-01-18" version="1">MSGANVDPNRVEKTIKNEVEEKFEDTHPSNEDPEQSIKKEEGEVYILGHEIDYPGFDRNEQITLELLADTINDTIKYQIEDTTRNLEDNGLDNDLRRDAYIRRIKALEAIKIKVLSNPHTLSYTVDINEPINSEAYQTTMKSIEGDIDEFLLKTSFRASKSTMFSVFGGPGYEELRNYELMNSIETYRGLSWNT</sequence>
</entry>
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    <accession>A0A1E3P5E2</accession>
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            <fullName evidence="4">FHA domain-containing protein</fullName>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
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            <taxon>Eukaryota</taxon>
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    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ60661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5E2"/>
    <dbReference type="STRING" id="683960.A0A1E3P5E2"/>
    <dbReference type="GeneID" id="30200380"/>
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    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
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    </dbReference>
    <dbReference type="InterPro" id="IPR051176">
        <property type="entry name" value="Cent_Immune-Sig_Mod"/>
    </dbReference>
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        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 170 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715:SF37">
        <property type="entry name" value="LD47843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="481"/>
            <end position="499"/>
        </location>
    </feature>
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        <location>
            <begin position="131"/>
            <end position="187"/>
        </location>
    </feature>
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        <location>
            <begin position="45"/>
            <end position="105"/>
        </location>
    </feature>
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            <begin position="334"/>
            <end position="464"/>
        </location>
    </feature>
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        <location>
            <begin position="70"/>
            <end position="84"/>
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    </feature>
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        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="502" mass="57339" checksum="88CF91EFA3CC1412" modified="2017-01-18" version="1">MEVLNSNSNDDLQQMNQNEQQHRDGAQMAPRTMYLNIELERSLNNGFERKTTRSRSNSKVYRQSLPPPVNNNTKSSSSSHNGTSIQQKDSQDTQPTKPHSRDQYTHIIRLSSLNETFDRKTLAIPAYPEVLKLGRPNSSQKAPNIDNGYFDSKVISREQAELYVKCDQVFIRDKNSSNGTFVNTKKILQEEPVKENDVIDLGIDIDGNQSKHQHHRKISCKVDSIFVVPLNNGVSLPQVLNDLNNQDKVMKQKEVTPFDAAFFGDVNSDLDDLALGMDHDFLSGIFVNNNIGTSSNLTKSVKILMNQLHQEKVNNLKLQSVETFLENYRVELSRNEQTAKIRKLNKELSDYKKLAEVSKSQISEFEEKIGEITSKLESNEKTVLEKDEQIKELEASATKHNETITNELNSKIESQDALIKEKQARIESLTKEQQSIKSNYESNLKSLEDKLSTNSEDYQKLKKKYKSILDYKKQNDMKNDFYNLILISTGILIIGLLVLKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5J2</accession>
    <name>A0A1E3P5J2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_61210</name>
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    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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            <taxon>Wickerhamomyces</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5J2"/>
    <dbReference type="STRING" id="683960.A0A1E3P5J2"/>
    <dbReference type="GeneID" id="30200375"/>
    <dbReference type="OrthoDB" id="3898179at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051710">
        <property type="entry name" value="Phosphatase_SH3-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469:SF27">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="549"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="434"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="475"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="504"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="626" mass="70723" checksum="29EF929AF7FB6A75" modified="2017-01-18" version="1">MSRVKIKEIDSLFILRHAQRNDKVATTTSSIDFVANNNDTAANGYMITETYNPPLATFQSTGNKSNNGCVQAMSMGQQILNYIDHNIEESLIILKFHTSPYLRCIETIKFILDYFTKNHHDDSKTIRCIISIDQVLSEWLSTDLDINYYPPSDNGASLLSTAMQYLNSKLPFHRHTNLEIQLDLHNSYKHGNPGCFNESFIQQYSRLNNGLISIVKHETNNLSNESDSKDLVIIMTHGACVRSLVSKLLGRSLYVEIPLASLSIAKPLMEDSKHYYWQLLKTDIDTKLDNFQQIDLYSKHDPFQDFQTTFKQNVGTNQLNFKSLIPASKTENFNRIRSQSLLTPRPKHHDSDDEDNEDQSDDEGISFTARKRRPRSVTTSASTATDNQRRFRSSSLFGPVLKNPFFSDESLKSNNDDRKKMFIDQEDEIISPVQNLSKKPSQSNSSSFQNIHEDDGTFGGAKQDSNELVPDLGESFSSNNSSTNSLHEEMTNIVPGSFSKMVKPPSQNTSSSSTTLQNQPTTTSSTPLPQNSSSLFDKSLIEKTLQEINLTSQNEPDHKTGETGGDWLNFKKDTSTGSNNEGSNKFKIDLYGKKDRLRLNLYNNMNDEDEEDVQGSSGWFLGSNKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5K1</accession>
    <name>A0A1E3P5K1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_28618</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039936.1">
        <property type="nucleotide sequence ID" value="XM_019181516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5K1"/>
    <dbReference type="STRING" id="683960.A0A1E3P5K1"/>
    <dbReference type="GeneID" id="30198762"/>
    <dbReference type="OrthoDB" id="296793at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140312">
        <property type="term" value="F:cargo adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000425">
        <property type="term" value="P:pexophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000301">
        <property type="term" value="P:retrograde transport, vesicle recycling within Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048685">
        <property type="entry name" value="COG3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048320">
        <property type="entry name" value="COG3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007265">
        <property type="entry name" value="COG_su3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13302">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13302:SF8">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20671">
        <property type="entry name" value="COG3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04136">
        <property type="entry name" value="COG3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 3 N-terminal" evidence="10">
        <location>
            <begin position="62"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 3 C-terminal" evidence="11">
        <location>
            <begin position="224"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ60729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="689" mass="80688" checksum="F48217153207B3C8" modified="2017-01-18" version="1" fragment="single">HSDKSDHHTYDKRDLLFPYTQDEKQHLIKEYIDSYDYNVLLSTEDEKEIEDQEFSKYDAFLETCKFRKHNYDGLINKSSDIIQILSDLTVKYDTVTKETYEFQEQSDKLINEYNTYLKLNDEVKDYLHYFELLDTFTRRLNNPSPNIVRKSSFKRMLSQLDECIGFINEHKDFKDYELYNLRFKQCLVRALTLIRNYVVNNLKTIRDDLTTKISATKINSVTQDALIYTRFGSDSEFLYDVVADLVNRADNNDELEGLLSDCFNFYFGIRTRLIQPKIWEHLKLSVDDKKTLVQFSQGNISFFTKIFRDEFELFHKIFPRDSKDALLHWFQQISEPLNDTLRKSILRESSISSLCELATLLDKYYEYDEEEEEQFEANNLSTFNDYDKPRFENVDLGEVFHPILQDVQSRLVFRAQVYVDENIVRYRPTNKDFQIGHRRKSEVAQDDQDPDVSMSNSKNTLKDCYSPLSKGISLLSKMYQLVSSSVFDDMAHNIVHDCIISLRQAYNVGKPTIGKLDSELFYLKNLLMLRSQIQNFDIEYVSNETFLDFSGIGDIINKIRNGSSVFNDGGILELVRESVPKVVNNMIDARTELQTELRNIVHEFTEDAVEQIIEPISKANPETAEQDSMKLKDNIEASFPRLKSEIEIFIDNREIVTSLIDGIQVCIFLPLFWGPELTNQLLGTCYSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5X7</accession>
    <name>A0A1E3P5X7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome b-c1 complex subunit 6, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Complex III subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_78945</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UQCRH/QCR6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039549.1">
        <property type="nucleotide sequence ID" value="XM_019184810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5X7"/>
    <dbReference type="STRING" id="683960.A0A1E3P5X7"/>
    <dbReference type="GeneID" id="30202056"/>
    <dbReference type="OrthoDB" id="405848at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.20:FF:000003">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.20">
        <property type="entry name" value="Ubiquinol-cytochrome C reductase hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023184">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036811">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02320">
        <property type="entry name" value="UCR_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81531">
        <property type="entry name" value="Non-heme 11 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ubiquinol-cytochrome C reductase hinge" evidence="12">
        <location>
            <begin position="160"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="24"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="62"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="94"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ60342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="231" mass="26666" checksum="F2FD955DD6DB9C56" modified="2017-01-18" version="1">MSFFSGLIETFAPTLAFADNDAETVVDNSTRSTNESTPSSTTIEGKDENKEGDAKPNTPDADLSKEKSQQDEPKGEPKPAQQDEKVEEKKEEEPKEEEEEPKEEEETKEEEPKEEEPKEEESSEESSEETIEESEEGGDDEDEDDEDEDEDEDEDEEGGDPLDTLREECAQHESIKPYVHHYQECVERVTKEQEEPDYEDKDYKEDCVEEFFHLQHHLNDCVAPRLFHKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6F0</accession>
    <name>A0A1E3P6F0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein YTP1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_77656</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040206.1">
        <property type="nucleotide sequence ID" value="XM_019184592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6F0"/>
    <dbReference type="STRING" id="683960.A0A1E3P6F0"/>
    <dbReference type="GeneID" id="30201838"/>
    <dbReference type="OrthoDB" id="4005299at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018825">
        <property type="entry name" value="DUF2427"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018827">
        <property type="entry name" value="YTP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31685:SF3">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_6G12730)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31685">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_6G12730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10348">
        <property type="entry name" value="DUF2427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10355">
        <property type="entry name" value="Ytp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133694" description="Protein YTP1-like C-terminal domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="453"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="531"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="563"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="600"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2427" evidence="4">
        <location>
            <begin position="80"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Protein YTP1-like C-terminal" evidence="5">
        <location>
            <begin position="333"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="631" mass="71031" checksum="E9E90BF6F5D01147" modified="2017-01-18" version="1">MKLSKRLVIWWFALSVVAAMNMDHSMMTDDESEADVEISTSIGSNLSTIEPVAHEMKHQHGLPILETKLTPAERAYWEAYNTTTYFTIDSPKKSALWVHCFTLFATFFVLYPVSLVLNNVNSKWYLPTLLINLINIVISLICLKVFGASVPDIYPKNAYAKMSWILLFALITHFCLAVLYSASKWAAGIDLDGDEAYNQPFIPLDDLGNDSRHDPSNGTNVDLERMASGGSNSPSTLIDSHSDRENKYKDSIENQNGTQNLPFYDKDSLDLGNEDVEFNTNPPPLRSHSSFLARDHFLNKIFQSPVVQNLSGKFHVFISILFKCFNYPMLGYLFVYVITGIAVGNLFGKANRVFNLLAHFIKGGVFVTLGIFSLARYCGALSKSGLAWNKAVIFKNQVNTRSIWYKLTPHGMITMEGYESFLIFFYGTTNVFLEHLASPGGAWVAKDLQHVSIAFMYIGSGLCGLITEHILSEWRFNQVLVNTNINPTAIHAGSPGYSPNPFPVFTIFWTGILMSQHEQASEVSTMIHMQWGYLLSFGSFFRLLTFVLLMLVPNKNLNPARPFTELITSFALICGGVIFMQSTDQVVEALEYRGLTSMFILNVNVGIVALVMGWEMSLMIWKHWLQNKRQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6H8</accession>
    <name>A0A1E3P6H8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UBR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_77235</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039759.1">
        <property type="nucleotide sequence ID" value="XM_019184521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6H8"/>
    <dbReference type="STRING" id="683960.A0A1E3P6H8"/>
    <dbReference type="GeneID" id="30201767"/>
    <dbReference type="OrthoDB" id="5795902at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19677">
        <property type="entry name" value="UBR-box_UBR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040204">
        <property type="entry name" value="UBR7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047506">
        <property type="entry name" value="UBR7-like_UBR-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13513">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13513:SF9">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="6">
        <location>
            <begin position="74"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="4">
        <location>
            <begin position="74"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="231"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="234"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="447" mass="51635" checksum="83FEE5935FC78A3F" modified="2017-01-18" version="1">MASDEVKKELNQGSKETKQDQTKNTTDVDSNQQSNIKPELDSNPSSDSITAQDYISSQLELEREAKELMPYDPNQCTYSMGAIRQQIYACLTCLAKTGQTSGICYSCSIQCHSDHELVELFTKRNFTCDCGTTKMNHPCYLRSKIFDDIPSSSNSYNQNFKGHFCDCKQLYNPIEEKSNMLQCIFGETCQEDWFHDYCIMGLTHYEPPKQKDHGVNKLDQLGEPEIDAATQNIKNEEDKKEELMKNDQQEEENGEPVLDGFPNLDDFDCYICWKCINKFKDFFDKIKQNNDIVVTTVQRIPSENLQERNSIILQNQDGTFFKKRKIDYDYSIFLKTNYEENFKTLYNTTNDTTIKKILDEFQFLMNDDPIYEPPNDDEIDPEDDDNSSIYDLGAKAINSLPREQAIQGVQAFQDIKTKLKDFLAPFAQDGKIVGKDDVENFFKNIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6I4</accession>
    <name>A0A1E3P6I4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Plasma membrane iron permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_28966</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxidase-dependent Fe transporter (OFeT) (TC 9.A.10.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040252.1">
        <property type="nucleotide sequence ID" value="XM_019181563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6I4"/>
    <dbReference type="STRING" id="683960.A0A1E3P6I4"/>
    <dbReference type="GeneID" id="30198809"/>
    <dbReference type="OrthoDB" id="4364at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004923">
        <property type="entry name" value="FTR1/Fip1/EfeU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632">
        <property type="entry name" value="IRON TRANSPORTER FTH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632:SF2">
        <property type="entry name" value="PLASMA MEMBRANE IRON PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03239">
        <property type="entry name" value="FTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0410">Iron transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="42850" checksum="687B67C028938D21" modified="2017-01-18" version="1">MGSTNKVFNVAVFFVVFRECLEAVIVISVLLSFLKQSIGTNDLSLYKKLRKQVWIGSFLGVFICLVIGGGFIGAYYSLQNDIFGKSEDIWEGVFCLIATVMISFMGIAMLRINKMQAKWRVKIAKSLVEVPERKRDRFRIGYLAKRYAMFLLPFVTTLREEGLEAVVFVAGAGITTKGAKASSYPLPVVVGLIAGCGAGMLLYYGVSYTSMQIFLVISTCILYLIAAGLFSRGVWYFENYVFNKKTGGDASESGDGNGSYDISKAVYHVNCCNPELDNGWDIFNALLGWQNTGYIGSVLSYNIYWLCLIIVVGLMLFEEKRGHLPFCKNLKLRHLNPMYWIKNKKKNELTDAERDELFRKAQDIRFNEEGELEEGSSPVAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6I5</accession>
    <name>A0A1E3P6I5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Complex 1 LYR protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_77244</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039769.1">
        <property type="nucleotide sequence ID" value="XM_019184522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6I5"/>
    <dbReference type="GeneID" id="30201768"/>
    <dbReference type="OrthoDB" id="3977609at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="1">
        <location>
            <begin position="14"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="308" mass="36847" checksum="B50ABE79F5CB15C8" modified="2017-01-18" version="1">MVTLFKKFPGHREEVFSLYKALLRHARHLPGQDERIRLELMHKIRYKFRQGKISKSGVNVRKFLEEGHEMEQSLREFYKNPEQKLVQELLDAELPPTHRELVKARRTELLLKKYPPRKPKGDPLQPLEKQREGSYVNAHIKYSRRYGLMPKVIDPEILEHIIKPEALFKRAKTDIKIEEQKLAKGPYRVKKAEYMGVPYLRTMGPVDRRTSGKLLNQKISVIQLTNMELKAKENLFYCEMETRWEHLMKKIDPKYECFDWREFITDALDQQRKANQYVFEGHKQYANNEMKDELIQQQAKANMLMMIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6Z8</accession>
    <name>A0A1E3P6Z8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="7">PPIase</shortName>
            <ecNumber evidence="2 7">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_61204</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 7"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039844.1">
        <property type="nucleotide sequence ID" value="XM_019183127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6Z8"/>
    <dbReference type="STRING" id="683960.A0A1E3P6Z8"/>
    <dbReference type="GeneID" id="30200373"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000013">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="8">
        <location>
            <begin position="5"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="162" mass="17575" checksum="12464F6E767F7212" modified="2017-01-18" version="1">MSKVFFDVTSDGQPLGRITFQLYNDVVPKTAENFRALATGEKGFGYENSPFHRVIPQFMLQGGDFTRGNGTGGKSIYGEKFADENFSKKHTKPGLLSMANAGPNTNGSQFFITTVPCPWLDGKHVVFGEVVDGFDVVKKIETLGSNSGATRTQLLVSKSGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P776</accession>
    <name>A0A1E3P776_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_28480</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040281.1">
        <property type="nucleotide sequence ID" value="XM_019181488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P776"/>
    <dbReference type="GeneID" id="30198734"/>
    <dbReference type="OrthoDB" id="3978510at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000171">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF4">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="439"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="473"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="501"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="49"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ61074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="530" mass="57905" checksum="FD54EC6D73DB911A" modified="2017-01-18" version="1">MSTDVEKSQQILHTTKEDERSSLSSSTDYSYKFFKAPKGELSQFDTSDIALRSKINLINDALDEIGFTWYHFKLFCLNGMGYAVDSQLTLLQSSVATYVRYQFHQTYPSDTHALYAGLLTGAIFWGFGADLIGRKIAFNSSLLISAIFAILAGVMGNFGTYCLFVALSAAASGGNLVLDTTVFLEFLPSKNQWLVTLMASWWSVGQVIAVLTTWLFFSQDRFVCEGKNNCPGSENMGWRYVWIVNGGIVLVIAILRLTVIKLEETPKFLVCNQRDEQAVECLQRIAAKYNRPCSLTLEDLEACGEVTSNENFMNKPSIKTTYKLIIKHLKSLFANRIMATSSTLIAISWALLGISYPLYTNFLPTYLATRGANTSASTVGGVYRDSLISNSASTAGGLIGGAILYFCPSIGRRGVMVIGGLSSMALFFGYTAVRTRAENVGLSSAVFVAICIYYSCLYAYTPEVMPSASRATGNALCVALTRLTGCFVPLIAYFANTSSAIPVWICGACVGVIAVCALFFPFEPSKQRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7A0</accession>
    <name>A0A1E3P7A0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">KOW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_99813</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL27 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040506.1">
        <property type="nucleotide sequence ID" value="XM_019186700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7A0"/>
    <dbReference type="STRING" id="683960.A0A1E3P7A0"/>
    <dbReference type="GeneID" id="30203946"/>
    <dbReference type="OrthoDB" id="2365484at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06090">
        <property type="entry name" value="KOW_RPL27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.770:FF:000001">
        <property type="entry name" value="60S ribosomal protein L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001141">
        <property type="entry name" value="Ribosomal_eL27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041991">
        <property type="entry name" value="Ribosomal_eL27_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038655">
        <property type="entry name" value="Ribosomal_eL27_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10497">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01777">
        <property type="entry name" value="Ribosomal_L27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="KOW" evidence="4">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="136" mass="15378" checksum="9E1ED082AA0373A8" modified="2017-01-18" version="1">MAKFIKSGKVAIVVRGRYAGKKVVIVKAHDEGSKAHKFGHALVAGVERYPSKISKRAGAKKVAKASKVKPFIKTINYNHLLPTRYTVDVEEFKSAISTETFDEPSQREEAKKVVKKAFEEKYQSGKNKWFFTKLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7A3</accession>
    <name>A0A1E3P7A3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fumarylacetoacetase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_29080</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040445.1">
        <property type="nucleotide sequence ID" value="XM_019181573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7A3"/>
    <dbReference type="STRING" id="683960.A0A1E3P7A3"/>
    <dbReference type="GeneID" id="30198819"/>
    <dbReference type="OrthoDB" id="74910at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018773">
        <property type="term" value="F:acetylpyruvate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050163">
        <property type="term" value="F:oxaloacetate tautomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.850.10:FF:000003">
        <property type="entry name" value="Fumarylacetoacetate hydrolase domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820">
        <property type="entry name" value="ACYLPYRUVASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820:SF7">
        <property type="entry name" value="ACYLPYRUVASE FAHD1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="3">
        <location>
            <begin position="11"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="234" mass="25796" checksum="61412C89F90D586D" modified="2017-01-18" version="1">MSLSYIKNAGKILCIGRNYVAHIKELNNTKPSQPFYFLKPSSSILEPNAGPILIPRGTVVHHEVELAVILNKTLKNLDPNTFTNQDALDSIDGYALAIDLTARNVQDEAKKKGLPWSIGKGFDTFLPLSKFIPKEKLPDPYKAELHLEINGETKQQDLTSLMIFPIHKILSTMSSVMTLEKGDIILTGTPKGVGKVLPGDHVTAGLTYEGVEIAEGKLDLEVEQKPGPYEYKET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7C8</accession>
    <name>A0A1E3P7C8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Mannan endo-1,6-alpha-mannosidase</fullName>
            <ecNumber evidence="4 12">3.2.1.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_61886</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for normal synthesis of the cell wall.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>Random hydrolysis of (1-&gt;6)-alpha-D-mannosidic linkages in unbranched (1-&gt;6)-mannans.</text>
            <dbReference type="EC" id="3.2.1.101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyl hydrolase 76 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.101" evidence="4 12"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040536.1">
        <property type="nucleotide sequence ID" value="XM_019183266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7C8"/>
    <dbReference type="STRING" id="683960.A0A1E3P7C8"/>
    <dbReference type="GeneID" id="30200512"/>
    <dbReference type="OrthoDB" id="4187847at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008496">
        <property type="term" value="F:mannan endo-1,6-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007117">
        <property type="term" value="P:budding cell bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000006">
        <property type="entry name" value="Mannan endo-1,6-alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005198">
        <property type="entry name" value="Glyco_hydro_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014480">
        <property type="entry name" value="Mannan-1_6-alpha_mannosidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145:SF36">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03663">
        <property type="entry name" value="Glyco_hydro_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016302">
        <property type="entry name" value="Man_a_manosd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 12 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133705" description="Mannan endo-1,6-alpha-mannosidase" evidence="15">
        <location>
            <begin position="18"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="427"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="392"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="394"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR016302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ61329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="449" mass="50678" checksum="906437EDBC8AE000" modified="2017-01-18" version="1">MIVRKWFSLVFVTVVTSLELNTADFESIKNATSTIAKGVLDYYTGDDYGKPVGLFTEPYYWWESGGAWGSLLDYWWYTDDDQYNDLIKEALLSQVGQKWDYVPLNQSTTEGNDDQAFWGMAVMAAAERNFSNPPEDDPQWLYLTQAVFNTMAYRWDDEECAGGLRWQIFKWNSGYDYKNTVSNGCFFHLAARLARFTSNNSYVEWAEKTWDWMNRTHLIDDSGDTTDYYLIYDGVSTNDNCTVVHSLQWTYNAGLMLSGAAYLYNYTESELWSSRLHSLLQGVSIFFREGVMFEAACQSSQSCNNDQRSFKAFLSRFLGLTALLAPDTRDIIDWYLNSSAQAAAQSCSGGEDGVTCGLNWQSEGWDGYYGLGEQMSALEAIQNVRYLDKPPPYTQHSGASSEGNPAAGNHLLDEEPKPLHIEPGSKVGAAFITAFISLSLILACIWILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7N1</accession>
    <name>A0A1E3P7N1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_61983</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040633.1">
        <property type="nucleotide sequence ID" value="XM_019183291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7N1"/>
    <dbReference type="STRING" id="683960.A0A1E3P7N1"/>
    <dbReference type="GeneID" id="30200537"/>
    <dbReference type="OrthoDB" id="445556at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="271" mass="32298" checksum="0CA18829CEC9A48D" modified="2017-01-18" version="1">MQTSLKLLIRRASTINNSSANDPFYHSEWPSHKNPSPYDVLNIKKSEFDPKTLRKRYYQFAKIYHPDISKNSTLINHKGDRLSDDHKNERFKLLTNAYTLLKDEDKKNLYDQFRMGWETNEQILTRSHYRKPSPAYATQYDNYAYWNAGSWEDYQNLREDDPTTRSEKMKILAALVGIIIFSATIQGWMLLNSVEKTLMESKKVHDDTEADLGLVYMNYGYDQSRISRFRRFLWFRTFGLYRDKESLDESHLENEKILKQVFGKDYVEYDD</sequence>
</entry>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
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        <location>
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            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="152" mass="17339" checksum="54254A9BC08244EC" modified="2017-01-18" version="1">MSRVSTPGSRRTNAKSRRHSELVDSSTEINDNEEAHNESEESPDPKRRRSTRNSEINYSLTKRNARSNGKSHIKAENQPEEEIIVKKTKDATLIEDTKKLERHRSEESNLSQVDKLGKLAAKHSLLTQELFHLQQFTSLASWDPTAPPPKKL</sequence>
</entry>
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    <name>A0A1E3P7T7_WICAA</name>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Phaffomycetales</taxon>
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    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ61428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019181608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7T7"/>
    <dbReference type="STRING" id="683960.A0A1E3P7T7"/>
    <dbReference type="GeneID" id="30198854"/>
    <dbReference type="OrthoDB" id="409586at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
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        <property type="entry name" value="CARRIER PROTEIN YMC2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="21"/>
            <end position="106"/>
        </location>
    </feature>
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        <location>
            <begin position="117"/>
            <end position="198"/>
        </location>
    </feature>
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            <begin position="213"/>
            <end position="297"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="32826" checksum="D36E6CD78E58036E" modified="2017-01-18" version="1">MTEEASFPNLEDLNGQEGDKFRVVKDLFAGTVGGIAQVLVGQPFDTTKVRLQSDTTGQYKSTVDVIKKLIKNEGMLAFYKGTLTPLIGVGACVSIQFSVNEYMKRNVFSGSAHLNNSQYYLSGVASGLANSVLASPIEHVRIRLQTQLTGNLGPLDIIKKIYSNNGVSGLMRGLIPTAVREGHGMGMYFLTFEYLVKRDTLKNKIQRKEIPGWRLCLYGAAAGYAMWFTVYPADIVKSRLQTDNLTNPEYKSLKDCIGKIYKANGVGGFFKGFAPTILRAAPANAATFYAFELTMRLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7U1</accession>
    <name>A0A1E3P7U1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ61475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019185567.1"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="51" mass="6131" checksum="DFA313972D630CD9" modified="2017-01-18" version="1">MAKVEIVFPIEKNKTRISKWKTDSRIELNKKEFEDWIGLILDGNLMVYWFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P893</accession>
    <name>A0A1E3P893_WICAA</name>
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        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
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    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3P893"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="429"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="458"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="525"/>
            <end position="550"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ61629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="563" mass="62656" checksum="43428AD87B2C71F4" modified="2017-01-18" version="1">MKDHQFKHTNDQRRASLVYSQGKPALYSPQDFFIKRIIDVDNEGDEEEQVETRPLLQEDPYFYTEESSNRRSRELLSEERNLLQNNNLITEDSNSDIIPSTWETAVKNGLLKTSHHLEINALTKSALPLVITFLLQNSLSLASIFSVGHIGKAELAGITLGSMTANITGFAAIQGLTTCLDTLCSQAFGAGKFELVGLQFIRCAIFAITCFIPISIIWVWFSTNLLSLMVKDYELVLIAAKYLQIVSIGMPGFILFECGKRFLQCQGVFHASTYVLLVCAPLNALLNWVLVWKFELGYIGAPIAITINYWLMPLGLLCYTIYDQKVLKCWPQNFQINQAFTNWSKMIHLALPGVVMVEAEFLGFEIVTLMASRLGTTELASQSVVCSIAALAYQVPFSVSIATATRVANYIGASLVVNAKKCCTASMNLGLIIGIINSLIILKFRYQITSFFTNDEEVISKVAGVLPLLSIMEIIDCLNACSAGCLRGQGVQKIGSYINIFSFYVVGLPISYIMTFKYDMNIAGLWIGIISGLISMCLLQFYIVFIGADWDKIVVDARKRNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8G0</accession>
    <name>A0A1E3P8G0_WICAA</name>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycetes</taxon>
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            <taxon>Wickerhamomyces</taxon>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ61504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040711.1">
        <property type="nucleotide sequence ID" value="XM_019185570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8G0"/>
    <dbReference type="STRING" id="683960.A0A1E3P8G0"/>
    <dbReference type="GeneID" id="30202816"/>
    <dbReference type="OrthoDB" id="4084551at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="Pma1"/>
    </dbReference>
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        <property type="entry name" value="Psg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133734" evidence="3">
        <location>
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        </location>
    </feature>
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        <location>
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        </location>
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        </location>
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        </location>
    </feature>
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            <end position="106"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="383" mass="43850" checksum="E85574367B46C4D0" modified="2017-01-18" version="1">MKLLYLISLATAASSAAIETRYRKVRVPTSTTSSSSEIPKPWIRTIYSSVKEIITPTVIEGVTFSGKPSKETDQPLPWVSLNKDGSPKTIKPQIKNGQTKNPSPTYSTYFQTASTTTYSYDDLKAHNMDKDATHEEVIFIDEDKTYVSLNPLIRCTPDRFYMKGLARNVESAPFCTPRENSQLKLGKTYFVTWYTKFFPDNVDKVRIHLSYVKESLREKGMHKREVDSAFFTSDWIENLDGYFPLDIQEDWLLGKFEQNVGISIQPNSITDDEFNLLTNATIFKILKGPKVAKKNKETRKLEDEGISNDSAYYIMLSIPTVVCISAIGMYIFIWLTRNDRDISAIRQKVWKSKHKVLGKFKPKSNNKKYSELPQFSKDSNKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8R0</accession>
    <name>A0A1E3P8R0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit beta</fullName>
        </recommendedName>
        <alternativeName>
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        </alternativeName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040464.1">
        <property type="nucleotide sequence ID" value="XM_019186249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8R0"/>
    <dbReference type="STRING" id="683960.A0A1E3P8R0"/>
    <dbReference type="GeneID" id="30203495"/>
    <dbReference type="OrthoDB" id="269919at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002183">
        <property type="term" value="P:cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000008">
        <property type="entry name" value="Translation initiation factor 2B, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051855">
        <property type="entry name" value="eIF2B_beta_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ61257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="391" mass="43400" checksum="6D985524276C59CD" modified="2017-01-18" version="1">MRVISAARWNNPDHLIEQIRQLGKTLINAQPREFTCGNVIRRILAIIRDEVVESNINESNAPMISSMFSLLSTNIEKKEPAPPPPQTNPRIHHSKNHDLRSIIIQGIRDLIDEISNIDEGIEAMSVDLIHDNEVLLTPTPDSKTVLKFLLKARQKRKFTVLVTECFPNRTSIAHSFAKELSDAKIETVVIPDSHVFAVMSRVGKVIVGARSVFANGGTVSSAGVAAVCECAKEHKTPVFSVAGLYKLSPTYPFDVENLIEVGNSGKVINFSDSNLVQHADITNPLFDYIPPEHIDIYITNMYVLILLRKILSLLLTFIAVVVMPQVSYIVLSLTITRVKMLIWISSIDFSKFNESCALKSVRKLSLLGSNESKILFSALCLARPNFSIVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P903</accession>
    <name>A0A1E3P903_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_25936</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041100.1">
        <property type="nucleotide sequence ID" value="XM_019181208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P903"/>
    <dbReference type="STRING" id="683960.A0A1E3P903"/>
    <dbReference type="GeneID" id="30198454"/>
    <dbReference type="OrthoDB" id="4091477at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="124" mass="14824" checksum="BFC325AC5EBDDB6A" modified="2017-01-18" version="1" fragment="single">CKICEKVIRRDMSRHMRIHEEVSRFRCVYPRGNCAHKTGFFNRQYDFKKHLLHFHFEFDDGEVKKFYSLNEKLPHWGTCTCGVRFTGGDWLNNHILTKDPQKLCSHLKRLKELESSSIVPSRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P926</accession>
    <name>A0A1E3P926_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_59990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041122.1">
        <property type="nucleotide sequence ID" value="XM_019182913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P926"/>
    <dbReference type="GeneID" id="30200159"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="209" mass="25187" checksum="3A90DB0075CCFC33" modified="2017-01-18" version="1" fragment="single">NEEIGEEKVCKVLKDKDGNHIEPDSFRRLNKKRQRREEEVHTRTPTYPVEYGDETNEDKTKRYAKFWAYIENINKHKPGYLETLHLWNLGKLEHRYFSNNGKQECCGCKRQNETIYHVFEKCEFTHYLWEHLGFKRVASSPFSLTELTTPTHEFTENEKVLRKMSLWIDALIQTRITRKNDRTCEEAVSATKYKMRSIQQKYGFRGEWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P927</accession>
    <name>A0A1E3P927_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_86946</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041126.1">
        <property type="nucleotide sequence ID" value="XM_019186078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P927"/>
    <dbReference type="GeneID" id="30203324"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="57" mass="6383" checksum="53EF60FEAD0DFAA5" modified="2017-01-18" version="1" fragment="single">MSLFPSSTASHNHDPAFVICSSNSPKKEKHVWLLDHDHGTQDSSKSLDRITICYTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P977</accession>
    <name>A0A1E3P977_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Multivesicular body sorting factor 12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_59853</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040981.1">
        <property type="nucleotide sequence ID" value="XM_019182886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P977"/>
    <dbReference type="STRING" id="683960.A0A1E3P977"/>
    <dbReference type="GeneID" id="30200132"/>
    <dbReference type="OrthoDB" id="5344687at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032509">
        <property type="term" value="P:endosome transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019014">
        <property type="entry name" value="Mvb12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42089">
        <property type="entry name" value="YALI0F09427P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42089:SF1">
        <property type="entry name" value="YALI0F09427P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09452">
        <property type="entry name" value="Mvb12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Multivesicular body sorting factor 12" evidence="1">
        <location>
            <begin position="12"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="140" mass="15931" checksum="75A9F196B83900B6" modified="2017-01-18" version="1">MEGEKAHPLLAQIPLIVSPFYNPPKAVTLPFNYKPLPSQLPETNVTKDNLEAWIAECQHVKKEIKSYGTKQKEAYEEWHNNVVRRLAPGYLGGSNTLLTPQRRTQAATNHQSINETLESKTDTIKDENDIDRAFSTMNIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P978</accession>
    <name>A0A1E3P978_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GTPase-activating protein GYP7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">GAP for YPT7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_82109</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041173.1">
        <property type="nucleotide sequence ID" value="XM_019185335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P978"/>
    <dbReference type="STRING" id="683960.A0A1E3P978"/>
    <dbReference type="GeneID" id="30202581"/>
    <dbReference type="OrthoDB" id="10264062at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032889">
        <property type="term" value="P:regulation of vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000005">
        <property type="entry name" value="TBC1 domain family member 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF502">
        <property type="entry name" value="SMALL G PROTEIN SIGNALING MODULATOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="4">
        <location>
            <begin position="371"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00072091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00082648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="699" mass="82361" checksum="53B634D5C7443108" modified="2017-01-18" version="1">MSAKSFPPGKVDLIYSKSKVYVHPNKNSKENIPGFLYFIKESSISTDRELLFGWTPEHYLTQSDLEVFQKVDLDSINVNLPSNFKNHHNFIKRPSTFGSFSFSISLGNLFSIQIRPPSLGWWYGSIIINTRSSSDKLPVVFFHDDECPSTQREQSLKNKNFDPFGDDNGLFWGGLQMLDFLKNVAVVEKSSLENSILLINPTLMDLNNFSPTNIADDNNAKKNPFDFKKMLNDARWSVLETLARVTKFTKKSVDNVLDESPLPIKNLLKNPEVQRVNDEFDTARVYLAKWAMGVQEEAEKTRKQIILDENSRDLLSKELGLNFDKLLPEEILQAHERHREVSKQEWDSFFDHTGRLLITVNEVKNRIFHGGLNQVVRSEAWLFLLGVYPWDSSSAERYELNKVLRRDYETLKSKWQDDKEIQQDEYYKDQKFRIEKDIQRTDRNLEIYKNHGTEEGEDEGDDDDFDVSNVRNPHLFKLREILLTFNQYNDKLGYVQGMTDLLSPIYVTLQDDSLSFHCFVRFMDRMERNFLSDQSGMRDQMNTLNELVQFMLPELYIHLEKCDSNNLFFFFRMLLVWFKREFIWDDVLRLWEILWTDYYSSQFHLFFALAILQKNEKIIINHLRQFDEVLKYINDLSMTYKLNDLLTRSELLFLKFRKMVEIIDRSSEPSDAKSPINDNLRLLLSKELVIQKETTRVQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P994</accession>
    <name>A0A1E3P994_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Required for respiratory growth protein 9, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_24134</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for respiratory activity and maintenance and expression of the mitochondrial genome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRG9 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041168.1">
        <property type="nucleotide sequence ID" value="XM_019181018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P994"/>
    <dbReference type="STRING" id="683960.A0A1E3P994"/>
    <dbReference type="GeneID" id="30198264"/>
    <dbReference type="OrthoDB" id="5578174at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010487">
        <property type="entry name" value="NGRN/Rrg9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13475">
        <property type="entry name" value="NEUGRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13475:SF3">
        <property type="entry name" value="NEUGRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06413">
        <property type="entry name" value="Neugrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="72" mass="8615" checksum="27B4B6D6B3221748" modified="2017-01-18" version="1" fragment="single">EWKRQKYALGEKFKGKAWNPVKKLSRDEMESVRLLKRELPDMTSKDLSDHFKISPEAIRRILKSKWTPSLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9H7</accession>
    <name>A0A1E3P9H7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">37S ribosomal protein S35, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_26313</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041272.1">
        <property type="nucleotide sequence ID" value="XM_019181246.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9H7"/>
    <dbReference type="STRING" id="683960.A0A1E3P9H7"/>
    <dbReference type="GeneID" id="30198492"/>
    <dbReference type="OrthoDB" id="10052321at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021036">
        <property type="entry name" value="Ribosomal_mS45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28158">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S35, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28158:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12298">
        <property type="entry name" value="Bot1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="40698" checksum="BEB58BE6F6EEF4EB" modified="2017-01-18" version="1">MLTSHQSLIGGLKQQVTAVSPATQQQIRHFSRRRIAYPSYKAPKLGRTHKKDHRTTLRYQMQCFLGKKNYKNEYLDNRYFDAPKDHRPNYITPFSEQGSPLVDYKTGQVKNLKGEVVEGGFVPTRNFEDALRPFPHNSHCKTGFNLSKRDRDQIYKKIAVDGIPAQKVAVDMGIKIPRLEATVKLREIEKKWGKRKLITPELKKMSETMYKMFPLFSTRNTRENLSEIPVPEKTLSSRFMTIAESEPFGPIEASKVYDLPPAVDLLKKAAEHGEHSETTLDPKEEARLQREQFKKKFLFAKKKTDRYAFEFKKAKVGEVGFRYGTTLRDNKKDRKIDFDNSGKMIYALPQSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9M9</accession>
    <name>A0A1E3P9M9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_60183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041326.1">
        <property type="nucleotide sequence ID" value="XM_019182953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9M9"/>
    <dbReference type="GeneID" id="30200199"/>
    <dbReference type="OrthoDB" id="10617512at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="148" mass="16462" checksum="4B947F57BD46DABC" modified="2017-01-18" version="1">METLKGVRIFGYGTIIPMALIKETEQLPKQQDSVNGSFQSTACTRTPIEMEIDRKVPTNTDSLKNFTHDNENTTTWSGVGSPTRINSSNNIQTLDQICKKRENSDNNTNNQGINNDLKNGGVLFNEKLIGQIIEKAEFGRVVMGIDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9P9</accession>
    <name>A0A1E3P9P9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_26104</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Sodium-phosphate symporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041328.1">
        <property type="nucleotide sequence ID" value="XM_019181229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9P9"/>
    <dbReference type="STRING" id="683960.A0A1E3P9P9"/>
    <dbReference type="GeneID" id="30198475"/>
    <dbReference type="OrthoDB" id="260807at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101:SF80">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="388"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="435"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="522"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="561" mass="61785" checksum="B4001139E70D4C42" modified="2017-01-18" version="1">MAGRLHQFDYIVALSMIFAFLDAWNIGANDVANSFASSVSSRSLKYWQAMILAALMEFLGAVLAGSRVSDTIRSKIIDISTFKDDPAVLLLTMACALVGSSLWLTLATSFGLPVSATHSIVGAVIGSGIAAVGAKNIHWGWNGFSQIVASWFIAPAIAGCFASILFLISKYCVLERKHNLRNGLILMPILIFITFSILTMLITWKGAPNLHLNNLSDGAIAGSIVGVGAVASIIYFIFLFPYFRRRLIFQDWRLKWYHVFLGPTYYFAPTDDIPPIPKGHMLVPDYYADLQNLESKYNNDLDVERQQTAASSTAASPNAPLEKKQTTKQLWWSLAKQPKQWPRLIWLMLIHGWTQDVISNQMHATGALSGNLKDMHARSKYYDNKIEYLFSLLQAITACTMSFAHGSNDIANAAGPLSTIWNIYTTNKVDKKADVPVWVLCYTAAALVIGVWTYGYNIMRNLGNRLILQSPSRGFSIELGAAVTTVMATQLAIPVSTTQSAVGATVFVGLCNKDIKSVNWRIVAYCYFGWIFTLPCAGIMAGILNGIILNAPNKGQLYQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9V0</accession>
    <name>A0A1E3P9V0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Vesicular-fusion protein SEC17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_66466</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SNAP family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041378.1">
        <property type="nucleotide sequence ID" value="XM_019184043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9V0"/>
    <dbReference type="STRING" id="683960.A0A1E3P9V0"/>
    <dbReference type="GeneID" id="30201289"/>
    <dbReference type="OrthoDB" id="9984275at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005483">
        <property type="term" value="F:soluble NSF attachment protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035494">
        <property type="term" value="P:SNARE complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd15832">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000049">
        <property type="entry name" value="Alpha-soluble NSF attachment protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000744">
        <property type="entry name" value="NSF_attach"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768:SF8">
        <property type="entry name" value="ALPHA-SOLUBLE NSF ATTACHMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768">
        <property type="entry name" value="SOLUBLE NSF ATTACHMENT PROTEIN SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14938">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00448">
        <property type="entry name" value="NSFATTACHMNT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="32935" checksum="2616575614E112CF" modified="2017-01-18" version="1">MSDPESLVQQADKKAASKQSFLGGIFGSSKAYQLEEASDLYVQAANIYRLRKQGQKAGECFEKAAKAQLQNDSKDEAANTLVDAFKSYKSEDAASAARVLEQAIEFFTLRGQFRRAANFKNDIAQIYEEELFDIPKALQSYQDAGDWYESDNAQALANKAFLKCADLNALDGKYIEANEIYKKIVKNSLNNNLSRWSLKDYFVKVVLCYLAADDKVAANKYLEETLTLDSSFQSTREYKLLRDVVDAVGEGDAQKLANTLYEYDQFSKLDKWKTTILLKIKNTIIEADDDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA94</accession>
    <name>A0A1E3PA94_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_49712</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Siroheme synthase involved in methionine biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>uroporphyrinogen III + 2 S-adenosyl-L-methionine = precorrin-2 + 2 S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32459"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58827"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.107"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041495.1">
        <property type="nucleotide sequence ID" value="XM_019182637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA94"/>
    <dbReference type="STRING" id="683960.A0A1E3PA94"/>
    <dbReference type="GeneID" id="30199883"/>
    <dbReference type="OrthoDB" id="508204at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004851">
        <property type="term" value="F:uroporphyrin-III C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11642">
        <property type="entry name" value="SUMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000006">
        <property type="entry name" value="Siroheme synthase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.950.10:FF:000005">
        <property type="entry name" value="Uroporphyrin-III c-methyltransferase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012066">
        <property type="entry name" value="Met1_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050161">
        <property type="entry name" value="Siro_Cobalamin_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028281">
        <property type="entry name" value="Sirohaem_synthase_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790">
        <property type="entry name" value="SIROHEME SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790:SF6">
        <property type="entry name" value="UROPORPHYRINOGEN-III C-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14824">
        <property type="entry name" value="Sirohm_synth_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036555">
        <property type="entry name" value="SUMT_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75615">
        <property type="entry name" value="Siroheme synthase middle domains-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="2 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Siroheme synthase central" evidence="14">
        <location>
            <begin position="134"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="13">
        <location>
            <begin position="295"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF14824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ62288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="556" mass="61721" checksum="0B9ABF7BD81BF536" modified="2017-01-18" version="1">MSILTSLNCQDEVHLFVGVSGLTHSRVQSVLSNKAHPIIITHDPNVSIPTNLKKLVHENKIPLYERQFDLETDLLTLGRSKVGKIVDRLYVNLSSNYSIFKQQIFEKCQIYRIPINTTESSDLSTFTLLSSYSDGDFQFGVTTSGKGCKLANRIKREIVSQLPKDLGKVCTTVGGIRQRIKEEDRVKLAELYKNDYEEFLDLESGEHEEDSVQNSRFNTFISEFNMTEAQKKLQRSRWLSQVVEYYPFSKLASLSIDDLSTAYHNSNDNEQTNNNQGATLSQTSKTNLETKKGSISLVGAGPGSVSLLTLGALSEINIADIVLADKLVPQQVLDLIPSKTEKFIARKFPGNAEAAQEELLQMGINALREGKKVVRLKQGDPYIFGRGGEEYKYFEEQGFTPVVLPGITSALSSTVNSKIPATQRDVADQVLICTGTGRRGVLPNLPEFVKSRTTVFLMALHRISDLVPELIAKGWDPKVPATIVERSSCPDQRITRTRLEDLVDAVEAIGSRPPGLLVVGYACEVLNKPLTERWVVEEGYESNDSTVSGILESLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA99</accession>
    <name>A0A1E3PA99_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_59949</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041079.1">
        <property type="nucleotide sequence ID" value="XM_019182906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA99"/>
    <dbReference type="STRING" id="683960.A0A1E3PA99"/>
    <dbReference type="GeneID" id="30200152"/>
    <dbReference type="OrthoDB" id="289721at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF15">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 12 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="243"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="188"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="497" mass="57956" checksum="F467689DA824C7C1" modified="2017-01-18" version="1">MPKEQQNDSNSMVTIKPNYSPSKKLHKRREIPKEDQYQMFDNDSDDDLDDTVLIYDVPLSQSLVQLTTHQKPIQPRLHSLASDTTHTSSIMSSQYSLDSESDLDTALFTDAKSIALEMSRSKSVLAIQESTMRRHLIANFDRPKSLDSSLPSSHLKEKFISGTRQTDLPPKDKLETAKHLKEFQDIKTKAIRNELKNEKETLKELEKRRKQKAQDLSSWQNSVIPDFKKQVQLAETRELWWRGIPPKLRQFIWTEQIGIHLPSEYLSNLKTKSLKLIDLQDDLVQTIDKDIRSIFPDMPIFQTSLYEKLQLILVMYSQHKSYQRGLSTLAAVLLYNIGDFESTFACLLGLLQRTVMKLLYNNEKDQFNIEASSFLRTFQKKMPELYHHFQLKLKMEPWTYLEPLIKPIFSNHLPLEIVSSILDVYIFEGDSILWRCVLGLFKKIGYKLFGDEDEILDVIGWNALNKINKGKSQWYKYLDVGDEVEFINTVREVLKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAG1</accession>
    <name>A0A1E3PAG1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_76551</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041588.1">
        <property type="nucleotide sequence ID" value="XM_019184404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAG1"/>
    <dbReference type="GeneID" id="30201650"/>
    <dbReference type="OrthoDB" id="3997928at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015159">
        <property type="entry name" value="Rec107"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09074">
        <property type="entry name" value="Mer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="372" mass="41647" checksum="92A59D9EB2FCC284" modified="2017-01-18" version="1">MSNSTKLAGADNGNGGKPMEDSVSLMLKDLTHLEDTDESDGDEELNENEEIDEEAINKELVTWAGRLELESIDLREKAKALTSQMTQNSESIKNTAQDLKSEFKSWKETSNKSLDGAIQTFSKASASFSNASKVLSSLKGKDKSTTSDIQKILETLKSAKATIESLQVSHTNSSKKQVNDITNSVLSKKIQTVINGIQNLTNHVVNVEEARKQNDKNQKTINDTLEKCLRRIEDIQNLQVDFNRELKSLSRSQASLSLEFYKFKTINRRNDENQITTFTPESSFNDSRSDSALNHQLPSSLPTSNANTSQQHDQSQIPPQDDNSTQTTKTFQKPLSFPLPKRRGRPPKVQQRTSTFKPRTRSSQNKKEKGHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB73</accession>
    <name>A0A1E3PB73_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_60717</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VAC14 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041866.1">
        <property type="nucleotide sequence ID" value="XM_019183041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB73"/>
    <dbReference type="STRING" id="683960.A0A1E3PB73"/>
    <dbReference type="GeneID" id="30200287"/>
    <dbReference type="OrthoDB" id="5574975at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.900">
        <property type="entry name" value="Pex19, mPTS binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057546">
        <property type="entry name" value="HEAT_GCN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006708">
        <property type="entry name" value="Pex19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038322">
        <property type="entry name" value="Pex19_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026825">
        <property type="entry name" value="Vac14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021841">
        <property type="entry name" value="VAC14_Fig4p-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16023:SF0">
        <property type="entry name" value="PROTEIN VAC14 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16023">
        <property type="entry name" value="TAX1 BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23271">
        <property type="entry name" value="HEAT_GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04614">
        <property type="entry name" value="Pex19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12755">
        <property type="entry name" value="Vac14_Fab1_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11916">
        <property type="entry name" value="Vac14_Fig4_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Stalled ribosome sensor GCN1-like HEAT repeats region" evidence="7">
        <location>
            <begin position="739"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar protein 14 C-terminal Fig4-binding" evidence="6">
        <location>
            <begin position="897"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="24"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="632"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="678"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1118"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="119"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="690"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1145"/>
            <end position="1158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1188" mass="133977" checksum="7D7FFC0642A4C067" modified="2017-01-18" version="1">MSEKKAKLEEDDLDDLDDYLDDFADDILSKPPGFDLDTSKNQNGGSVTSETETVKESRPETTATASEEDAIAELLKKMEQESPEARNQLQDLINEVNSLDAPSSQAPDEHKTKDGNLKDTISSTLNRLKQSGSKVDKSIKDEQPDQMLQELLSQMNLDGDGGEGTDDFDITKLLADMLDNLASKEILYDPLKDLSSKYPKWIEEHKKEVSEDDLARYTKQSEIVQSIVAKFEDENYSDSNKEHRKFINDKLEEMQNCGNPPPDLTGGLEQSGLPGFNFNSGDLPDNMDKELEEGSALSTNWTPEHQLTSNACLQWKNIARGLNDRLYEKRKTAALELERQIRQCLAERKSDEKNDAQKPDKVKAIIDQLSKEYAYSVHQPNARNGGLIGLAAAAIALGQNEVAIYLEDIIHPVLACFGDQDARVRYYACESLYNIAKVSKGEILLYFNEIFDVLCKLAADSDPSVKNGADLLDRLIKDIVSEKATSYVSVIQNDPEGMYKSATQTTVKDINGQVLQIQPQQEPTAFSLPKFIPLLMERIYAINPFTRIFLVSWITLLNSVPDLELISYLPAFLSGLIIFLSDSHSDVRIITQSALDVFLHEIRRVSEIQKLVRDQKVKKQKDSKANEAITLDVEEIETSTPSKGPKTLPDSVEFPDAEKAKDNTSAIIGSDEENDKSLLKDTDISNAQDPVKEQQNKEEHDGELYIPGQDIHLDYPKIIEILISHLDSSEDKIQLVVLNWLEALLEISPSSFIPYLPKILSVLLMTMSNQEGSNSRESSKEVNNKLLELVTHLSEDDEKKLNYSLTINTVTMQFLNENEITRIAALDWLTMLHKKSPKQLLDHSDDTFTTLLKALSDSSEEVIHRDLQLLSEISFESDDENFSLFMLDLLELFKKDQKLLETRGNFILRQLCVSLDSKRIYQTLSKVLEKEEDVTFVGIIVQILNNNLITAPELSSLRKQLRNLEDKKDWELFSTLFRAWSHNAPAALALCLLAQAYELAYSVLQIFVDFEITVNVLVQIDILVQLLESPVFTRVRLQLLEPDKYPYLYKCLFGILMLLPQSSAFNTLKNRLSSISAINNVSKSQERENSSNKHIDASGLSKNIKWTSLLQRFRSVQQDHERSRLQSASSLKTRSALPKYSDTPSSEKTDPIEEKEKPNASLKTEPAKLAVRKLSGRSFGLKGFGGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB91</accession>
    <name>A0A1E3PB91_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_25882</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041883.1">
        <property type="nucleotide sequence ID" value="XM_019181198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB91"/>
    <dbReference type="STRING" id="683960.A0A1E3PB91"/>
    <dbReference type="GeneID" id="30198444"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000016">
        <property type="entry name" value="Solute carrier family 25 member 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="413"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="46"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="86"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="182"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="302"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="409"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ62676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="502" mass="56103" checksum="FACDE07691F59825" modified="2017-01-18" version="1">MSKESPEDQETRLKNLYKTIDIHGTGRVDLSTLNDAFNKSDHPLKNTPGAIETLFKSLDSNKDDVIDFQEFKTYILKAELQISEGFAKLDKDKDGKIFSHDLNSYLKLNKDENLKDFQSFIKLLQDDNSKSASYITYDQWRNFLLFVPRLNGSRLRTAYEFLNNNDVDLNSEGDVTVSNEILKGLGFFLAGGLSGVVSRTCTAPFDRIKVFLIARTDLSSTLLHKRAALASKAGEIPLDKIKSPLIKAATTLYRQGGLRAFYVGNGLNVAKVFPESAMKFGSFEAAKKLMSRLEGVQDTSELSRFSTYIAGGLGGVVSQFTVYPVDTLKYRVQCAPLDNKIHGNRLLVETAKQMYKEGGLKLFYRGVFVGVTGIFPYAALDLGTFSAMKKWYINREAKIKNCSTDDIIMSNFIVLPMGAFSGTVGATVVYPINLLRTRLQAQGTFAHPYRYDGFKDVLKQTVQREGYPGLFKGLVPNLAKVCPAVSISYLMYENLKRFLSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBD5</accession>
    <name>A0A1E3PBD5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Insulin-induced gene 1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_76452</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the INSIG family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041479.1">
        <property type="nucleotide sequence ID" value="XM_019184388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBD5"/>
    <dbReference type="STRING" id="683960.A0A1E3PBD5"/>
    <dbReference type="GeneID" id="30201634"/>
    <dbReference type="OrthoDB" id="205546at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025929">
        <property type="entry name" value="INSIG_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15301">
        <property type="entry name" value="INSULIN-INDUCED GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15301:SF3">
        <property type="entry name" value="PROTEIN NSG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07281">
        <property type="entry name" value="INSIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="30415" checksum="B6A98FC93E1609D0" modified="2017-01-18" version="1">MSQEAIDETDINLTKAPLYSIYDNDLDKDNDDNEIYNSLNSNIEKNKVNESHTIVHTPKPHLFKKILKLTASLLILSASGILYNQLGQHIHDNHILVPELASKPLDIGIKISNQLVFNGVAPKWLIYSIEGILFGLVQPIFDKYIFRSKPYSKDSSSTPFGIDTSSIVRAAVAFLGVSFAVRKIEWSSSIQASIAWSLLSPCLWLLLDGTLSGFFTGVFVASIASFSVIVFEGLPKNWYQDFEFTAVMLWLGNFFFFGLIIFGKIGRYLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBD8</accession>
    <name>A0A1E3PBD8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_66855</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041817.1">
        <property type="nucleotide sequence ID" value="XM_019184060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBD8"/>
    <dbReference type="STRING" id="683960.A0A1E3PBD8"/>
    <dbReference type="GeneID" id="30201306"/>
    <dbReference type="OrthoDB" id="4454541at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF10">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="46"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="84"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="131"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="660"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="157"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="660"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="685"/>
            <end position="700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="831" mass="93966" checksum="A0312E8C279E328D" modified="2017-01-18" version="1">MAPRATNKKEKPSSATPTTTTSTSAVTTKKDNGASKPAPSRRRAVACKSCHTLKVKCIPSDPDDLSSPCVRCIKTRRVCEIDTNYSRKKRKQPQQQQQQMHPQSITNSSQDRVNELEQKIEQLNNELLQHRSQMSSNSPQGSIHSNNQSPVHPGGQSFPNHFSPLSSYQQNTIGQRSSLSGTPISAERGNNSRGTKRPVNGGSSFEFINENDHFPKNGATDAKRAKVSKLTKAQQIEQLIGSTALSDVSNMVTQVAEERLRRVYSSHVDLIDSGILTIEEAQQRLKMYFEVIFAKYPFVDVPTDVETFKSVYPVLFNVVMAVTCVASTNDADFDKNLNIENIATQEVIHEVVVIGNKSIELLKSLLLLTIWYNMPELFHNRRYHIFSALCITMTQDLGLTGRPYYVVNKSEGSVLRSEVLEDPQKDEYRCLVLAVYSASIGYSLFLRRKLFVTWTPYLEDSYQHLRTSSELKFRSVAIFAKLTSLLEKIHSFVELKDQENLSFIQHFQRAIDFLKDQQTTMDSNVLLGFAYSVEAFLYSKFTSEELSFKCLHSTLQCLHHFSSLTPAQFCSVPLLIYGRLMYCFALLLKTSNCLSFEFDFSSIKRIMNTLDECNKIYKTNHLLVKTKLLFYFYLTTYSKNENAFNELANISSSETVVNANSQNSSISGTPTNTSSANQPPKTSLHQPVVQQSFEQQHQQHAPAGDAPGMATSHIPQGYPQQPHHPINTGNIPPHSHQYPDQYQHPLMHPQQHSHQQVNGPPMNVYPGISMPNTNSMLPQHDQLESHMEPVYPQLDVLSDATMKGFGDIPTELMGQDEFWKLFDYKDEQFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBG8</accession>
    <name>A0A1E3PBG8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 14">Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase</fullName>
            <ecNumber evidence="3 14">2.7.4.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_27075</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Bifunctional inositol kinase that acts in concert with the IP6K kinases to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. PP-InsP5 and (PP)2-InsP4, also respectively called InsP7 and InsP8, may regulate a variety of cellular processes, including apoptosis, vesicle trafficking, cytoskeletal dynamics, and exocytosis. Phosphorylates inositol hexakisphosphate (InsP6).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1D-myo-inositol hexakisphosphate + ATP = 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:37459"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:74946"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:37460"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate + ATP + H(+) = 1,5-bis(diphospho)-1D-myo-inositol 2,3,4,6-tetrakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:10276"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58628"/>
            <dbReference type="ChEBI" id="CHEBI:77983"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:10277"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
            <location evidence="1 14">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the histidine acid phosphatase family. VIP1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.24" evidence="3 14"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041847.1">
        <property type="nucleotide sequence ID" value="XM_019181335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBG8"/>
    <dbReference type="STRING" id="683960.A0A1E3PBG8"/>
    <dbReference type="GeneID" id="30198581"/>
    <dbReference type="OrthoDB" id="18042at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033857">
        <property type="term" value="F:5-diphosphoinositol pentakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052723">
        <property type="term" value="F:inositol hexakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052724">
        <property type="term" value="F:inositol hexakisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000830">
        <property type="term" value="F:inositol hexakisphosphate 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000831">
        <property type="term" value="F:inositol hexakisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052846">
        <property type="term" value="F:inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 1-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052843">
        <property type="term" value="F:inositol-1-diphosphate-2,3,4,5,6-pentakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052845">
        <property type="term" value="F:inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071545">
        <property type="term" value="P:inositol phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051516">
        <property type="term" value="P:regulation of bipolar cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110162">
        <property type="term" value="P:regulation of mitotic spindle elongation (spindle phase three)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000036">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11950:FF:000002">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037446">
        <property type="entry name" value="His_Pase_VIP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040557">
        <property type="entry name" value="VIP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750">
        <property type="entry name" value="DIPHOSPHOINOSITOL PENTAKISPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750:SF9">
        <property type="entry name" value="INOSITOL HEXAKISPHOSPHATE AND DIPHOSPHOINOSITOL-PENTAKISPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18086">
        <property type="entry name" value="PPIP5K2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="VIP1 N-terminal" evidence="17">
        <location>
            <begin position="61"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp fold RimK-type" evidence="16">
        <location>
            <begin position="272"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF18086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ62640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="950" mass="108840" checksum="A6D50CE3186C051D" modified="2017-01-18" version="1">MASIAPILEGFTPKTSSSENTSQIHLPPPAVETSSATGGSTRSSRKSSHSISKPQLPKIGKIGVCAMDTKVLSKPCRQILNRLIENGEFETVIFGDKVILDESIENWPTCDFLISFFSTGFPLDKAIAYVKLRKPYIINDLVMQKALWDRRLCLWLLDAANVPTPRRLEISRDGGPRVDDELKQKLKEKGVIIDRIDEPEWKMIDEDTIWVNGQTMKKPFVEKPVDGEDHNVYIYYAKKDGGGGRKLFRKVGNKSSEFDPNLTNPRIAGSYVYEQFMDTDNFEDVKAYTVGPEFCHAETRKSPVVDGIVRRNTHGKEIRYITPLSDEEKTMAKAVSKGFGQTICGFDLLRVGGHSYVIDVNGFSFVKDNMAYYDNCAKILRDMFYEAKKIRDVSKLATPSAPQEEKSQKWVLKGLVSVIRHADRTPKQKFKYSFKSEIFLSLLKGHKEEVIIREVSDLQIVLKAIKIAQEQKIEDASKLDLLANALEKKLHFPGTKIQLKPVLKSENEVEKVQFILKWGGEATHSAQYQASDLGEQSRQDMGLLNKELLNNVQVFSSSERRVLVTANLWANALLDGPIDDGFINIRKDLLDDSNAAKDLMDKVKKKLKPLLREGKEAPSQFTWPQKMPEPYIVIKRVVELMNYHQKIMEYNVTHGNFEKLQTKWCSGEDPELFVERWNKLFKEFITVEKVDPSKISELYDTMKFDALHNREFLEKIFQPVSEVERPSDSLLIDEYPEHLKLFVKNNNLPNAPPHTHSFLFNEQIFKYLRELYKLSQVLFDFICPQEYGITQNEKLEIGLLTSVPLGKKIISDLNEMIVNDKPGCVTYFTKESHMYTLLNILYESNIQFYINRNDLPELDYLSQIIFELYEDTSGKNHSIRFKLSPGCHTQDPLDVELDDKHYISCIPKISLTKHLDFDLVSQRLKSKFKKVNLPKKFTAVNISSPLSDPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC46</accession>
    <name>A0A1E3PC46_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_77144</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042193.1">
        <property type="nucleotide sequence ID" value="XM_019184507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC46"/>
    <dbReference type="GeneID" id="30201753"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="626" mass="72628" checksum="75F36D658129E4D5" modified="2017-01-18" version="1">MLAAAYSTIKYQSSLKSRYFMRLRLLESSASWSGSNVSRSISTTHGFDYTKILQKEPPELDYLDYGLKRDCTQGDYQTFCEKFLMESEPTKEAFLKVFFSTPTVYNPEFLQKSQSGSILTEDRILKDEQQIINTLPFKALSDLSFKEYQIRMEEIQQSEMSSLIDQEYKNFKELFLQSPNGAGYTKGQMLTTLIDTSAFKNYREVNTEEDVARQFQSKLLTPIENVFELSGHSDVSINYLCQIPLRDPRRTRNIKRADFENQLKKKLIIGEIKHHFDSLESELQGTKELSKKTLESITQCVRYCALSHCEYGFLTSLTETLLIQIDYDKCNYMGTDDDNIIQIRYYYLKNMDEKVTMKALMMSILMKQLKNTKSDNSQELQAYNQKKSENFLNFLLKSDRQFESDFQDMKNELALKMKPIGETFTLYVDHKSIKSSTEDQVLTFKIRKSDLIGDATADKAEETIVRIFDPMTMKSPYDEVKDNIDFSRNCYLSVLESLEYLDGSNVNKPVVVKKGFIIIRDRLNNNVLASGQFIAYALSKQTQQSSDVYSKQTLADLAKEQMKKLHSRGFNVSKNEIDLDPNQVILDEKNRVCLLEPLRSPFFQPISQEDQRNEQRMLDEAIAKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC48</accession>
    <name>A0A1E3PC48_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">tRNA dimethylallyltransferase</fullName>
            <ecNumber evidence="5 6">2.5.1.75</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_27240</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>adenosine(37) in tRNA + dimethylallyl diphosphate = N(6)-dimethylallyladenosine(37) in tRNA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:26482"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="EC" id="2.5.1.75"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 7">Belongs to the IPP transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.75" evidence="5 6"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042087.1">
        <property type="nucleotide sequence ID" value="XM_019181350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC48"/>
    <dbReference type="STRING" id="683960.A0A1E3PC48"/>
    <dbReference type="GeneID" id="30198596"/>
    <dbReference type="OrthoDB" id="775260at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00185">
        <property type="entry name" value="IPP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030666">
        <property type="entry name" value="IPP_transferase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018022">
        <property type="entry name" value="IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00174">
        <property type="entry name" value="miaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088:SF89">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01715">
        <property type="entry name" value="IPPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039110">
        <property type="entry name" value="IPP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="411"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="411"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="431"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR039110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="441" mass="51242" checksum="0E645E6C7AF1C547" modified="2017-01-18" version="1">MGPPKNVIVIVGTTGVGKSQFSIEVAKRFNGEIINADSMQMYKGADIITNKHPMEEREGIPHHVMNHINWDEEYFIHRFEKEALSTISDIHSRGKIPIIVGGTHYYLNSLLFLNKTIKVKEPEKDSSEKLTEDQKRVLDGPPEEVFKTLQIVDPVAAGKFHPNDTRRVRRALEIFYTTNKKASDHYEEQVESDQDKSGLRFNTLAFWLFAQKPILDKRLDERVDKMLSNGGMAEINELYEYYTKSEPKPDCERGIWQVIGFKEFLPWLEGERTDNELKISIEDMKSRTRQYAKRQVKWIKSLLAADLAKEKDHNYVKGGRLFLLDATDLSTWQSNVTERGLEITERFLEGKEDTLQQAPDGLEDMLPLEAEKEDKSTQWKHFTCDKCFNKDNTPVIAIGEKQWEIHLKSHRHKTNANRGKRKREYEEWLAKQQSTETIEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCF8</accession>
    <name>A0A1E3PCF8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_61014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042177.1">
        <property type="nucleotide sequence ID" value="XM_019183096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCF8"/>
    <dbReference type="GeneID" id="30200342"/>
    <dbReference type="OrthoDB" id="3979781at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="311" mass="35306" checksum="A4A64883185ECB0A" modified="2017-01-18" version="1">MFRSLVNRPFLRLGNPLIRPSRLIAFNFASGFPTNHASITKSTPPLTQSIVHQISIQRQFSTKPILNFTKPPRFNARPPSTLSLIWHLIPDPLKLFIAIMGTASFIMFIAAPLILIVLPPLFIGCYLTSRYWLKKRSKDMEGRWENLLKTHLTYEGSELDQMKLKDFTLERLVDAFETNEEGIADFFEGSGSANQHHSFSSRFELTGIETIDQDFRISKLGIQESISVMSFGLIDKDSRVGRIATVILTLRPKRVTTLLEAHTSSQDAVIEIKPVFNAFGKSFILNKGSDFGNSFSDRIIDIKAHNTRKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCH9</accession>
    <name>A0A1E3PCH9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-glycosylation protein EOS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_82650</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041879.1">
        <property type="nucleotide sequence ID" value="XM_019185416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCH9"/>
    <dbReference type="GeneID" id="30202662"/>
    <dbReference type="OrthoDB" id="2139606at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021100">
        <property type="entry name" value="N-glycosylation_EOS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28147">
        <property type="entry name" value="N-GLYCOSYLATION PROTEIN EOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28147:SF1">
        <property type="entry name" value="N-GLYCOSYLATION PROTEIN EOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12326">
        <property type="entry name" value="EOS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02070">
        <property type="entry name" value="NGLYCOSEOS1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="261"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="34221" checksum="03DF0C057EEFA335" modified="2017-01-18" version="1">MRISNSSVSLARDYSVQDSYRTGSYISLQALATDVVNIPDEQVPSYEEIMNQDQQNNQALRSVNQSRSNSIQEEVAVGSNNTSQERSNTQERQSLKVLGLNFLNARQHFALAICRDSVLIPSLYNSFKCFKQSYNFYYSPDPEFESQAMSILTRPSSSEHFIAGIWCIVAAYLSFSVIDGLMVRWIVTYQTGAAILRVLALSMLLITIEQATLAILSPDGFYSLHTWILISCILTVFYIVQNFVTSNLDLKNDKKPRSVDYYNITVFAVVPIGVASFISMIGLLRVLLILRLDLEREKYGLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU58</accession>
    <name>A0A1E3NU58_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_98271</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454224">
        <property type="protein sequence ID" value="ODQ56656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035863.1">
        <property type="nucleotide sequence ID" value="XM_019186645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU58"/>
    <dbReference type="STRING" id="683960.A0A1E3NU58"/>
    <dbReference type="GeneID" id="30203891"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000651">
        <property type="entry name" value="Serine/threonine-protein kinase RAD53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="274"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="317"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="330"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="342"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ56656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="358" mass="39886" checksum="3A604BC9CBF76A17" modified="2017-01-18" version="1" fragment="single">MGNTDGVSRELEILRRLDHPGIVRLRGFYEDDDSYYLVMEFVPGGDLMDFVAAHGAVGEEAAKEICKQILQAIQYVHSLEISHRDLKPDNILIAQDDPVLVKITDFGLAKISDTGTFMKTFCGTLAYVAPEVIDGRLSKITDSNKYSSLVDMWSLGCLVYVILTAHLPFSGSTQDQLYKQIQQGSYHEPPLKEAGVSKEARSFLDSLLQVDPRFRLTASAALSHPWIASFSQSNSQISLSQSQSQQQRLEAEKLHFDEFENKERMEKILEDHVFKVPAPPNPNKKKVPQKDDQASQASQQKTTQIAERSPSEDQSFDRTSLIENETSVVKSEDGGVLRPETEVTTQCPAGTSFTLTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUF1</accession>
    <name>A0A1E3NUF1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vacuolar protein sorting-associated protein 27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_37004</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS27 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035965.1">
        <property type="nucleotide sequence ID" value="XM_019182502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUF1"/>
    <dbReference type="STRING" id="683960.A0A1E3NUF1"/>
    <dbReference type="GeneID" id="30199748"/>
    <dbReference type="OrthoDB" id="957735at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033565">
        <property type="term" value="C:ESCRT-0 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904669">
        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140504">
        <property type="term" value="P:microlipophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903319">
        <property type="term" value="P:positive regulation of protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16979">
        <property type="entry name" value="VHS_Vps27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000345">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017073">
        <property type="entry name" value="HGS/VPS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049425">
        <property type="entry name" value="Vps27_GAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47794">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47794:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02809">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21356">
        <property type="entry name" value="Vps27_GAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036956">
        <property type="entry name" value="Hrs_Vps27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="VHS" evidence="13">
        <location>
            <begin position="20"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="12">
        <location>
            <begin position="169"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="229"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="280"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="325"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="458"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="233"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="459"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ56758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="480" mass="54367" checksum="1D8948537D0EB9E6" modified="2017-01-18" version="1" fragment="single">MSWFGSSTVTIDAQIERATDESIPFGETDLATSLEITDLIRSKQVLPKDAMRSLKKRLTSTKNPNTQLSTLHLIDTCVKNGGNHFIVEISSREFIDSIVSIIHDDKTNENVRDLSLDLIQNWAIAFKENFQFKYVVSTYEQLKSDGYNFPPIGSEISSNLFDSSAPPEWEDSDACMICSTLFSMLNRKHHCRNCGGVFCQSHSSKSLPLPHLGITEPVRVCDTCFDELSSKSSKGHKSKKTRRSKHQKEVSRARAQYDSDDDEDLKKALALSLRESQGYTQVPIPVIPPPQPTQQPKAQTVNEGDDDEDMKAAIAASLKDLEDKKLANQQQQQQQPIDNSPYANLLPQSNSQSYQSNIAPQAPSVNQNLNIISSNEESDIRQFASKVDYYKSNPSATTDMNELHDSYRRAVPLTPKLSSDLNESIDKYDKLVDMNSKIDTVMRLYNKLLDERIERESNYQRQYQQQAQYQPSSYQQYPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUF2</accession>
    <name>A0A1E3NUF2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_65639</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035963.1">
        <property type="nucleotide sequence ID" value="XM_019183956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUF2"/>
    <dbReference type="STRING" id="683960.A0A1E3NUF2"/>
    <dbReference type="GeneID" id="30201202"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN SMUBP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="236"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ56756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="754" mass="85280" checksum="C9E7AE0FAF18E1D0" modified="2017-01-18" version="1">MPKKKTPDGEVRDLISYIKGEKRMQTDESSEYYEREHFEREEDDSPKSYFKDELPNNEFLVKEDGNIETMVDYQRFISKCIYYEIGLDGHIGSNAKIENEEEGRFSTNVVIDGQIKKALGFNGRPFESTNQAFFLVTDGEFRCCKIESYDFGDDVLEVKATLFPWEESSAFKEMVQNYQGSKFRIVPCPNSLNVAIFRLRDLPYSKFQNLFLSKPLAIKEASDEAPKEKPLLVDPAFKNLNDSQQRFIKSALSNPLTVLRGPPGTGKTETIFQFIINYLERARSNELFERYELPNVAVKENGVKKKDLKPRVLVVAGSNVAVDNILAKLIKLNKIAPLRILSSAQKDGYDRESEFGPYCLHNIVLSQYIEENPDQLGVGFSGVQDPEKFADNLMGDSSNWKLAKKSLEVVLNAEVILTTTYISASALLKKVNIDCIIMDESSQVSEIDSILPLAREEIKKVVLVGDENQLQPFAGNSDATKSSLFDQIIQSEETFGQWIQMLDTQYRMHPAISAFSIEKIYENKLKDGVDANDRILDNGPGPLVFVDYWSKYQVIPELKEMKNGKSFLNRGEAKLIVEIIKKLMYSTSLKLNEIGIVTPYAAQKDHIAHVLGKDRLINPRGDRALGDFASSEGFVRKRKETMKVVNGIMIGSIDVFQGHEKPVIIFSTVRSNDDGETGFLKSKRRLNVGITRAQYSLIMVGNYKCLQDSDPFLDDMLRHFETRGRIIETDEFLKEKWTNDGGDTTASSAATGIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUR2</accession>
    <name>A0A1E3NUR2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">allantoicase</fullName>
            <ecNumber evidence="3">3.5.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_59279</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Utilization of purines as secondary nitrogen sources, when primary sources are limiting.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>allantoate + H2O = (S)-ureidoglycolate + urea</text>
            <dbReference type="Rhea" id="RHEA:11016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:17536"/>
            <dbReference type="ChEBI" id="CHEBI:57296"/>
            <dbReference type="EC" id="3.5.3.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Nitrogen metabolism; (S)-allantoin degradation; (S)-ureidoglycolate from allantoate (aminidohydrolase route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the allantoicase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.4" evidence="3"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035977.1">
        <property type="nucleotide sequence ID" value="XM_019182836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUR2"/>
    <dbReference type="STRING" id="683960.A0A1E3NUR2"/>
    <dbReference type="GeneID" id="30200082"/>
    <dbReference type="OrthoDB" id="10266039at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004037">
        <property type="term" value="F:allantoicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000256">
        <property type="term" value="P:allantoin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000078">
        <property type="entry name" value="DAL2p Allantoicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000059">
        <property type="entry name" value="Probable allantoicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00813">
        <property type="entry name" value="Allantoicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005164">
        <property type="entry name" value="Allantoicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015908">
        <property type="entry name" value="Allantoicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02961">
        <property type="entry name" value="allantoicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12045">
        <property type="entry name" value="ALLANTOICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12045:SF3">
        <property type="entry name" value="INACTIVE ALLANTOICASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03561">
        <property type="entry name" value="Allantoicase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016516">
        <property type="entry name" value="Allantoicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Allantoicase" evidence="8">
        <location>
            <begin position="27"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Allantoicase" evidence="8">
        <location>
            <begin position="197"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ56770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="339" mass="37720" checksum="C67C1A6973BB0BB0" modified="2017-01-18" version="1">MSQILSEQEFKDQVVSLYTDVVGEKVGGSIVNFSDEWFAAAENLIKPKAPIRDATKFVYSGAWYDGWETRRHNTEEADWVTIKFGVSSAKIIGTEIDTAFFNGNHAPAITVECTHLEEGQSLDHANWDLVIEKTECGPSQRHFFKRSSITEKSYTHARLKQYPDGGIARFRLYGEVVPHLDSIKANSIIDLASVGFGGVAVKCSDQHFGSADNLLLPGRGHDMSDGWETKRSRQPGHVDWVIIKLGAKTNINEIVIDTAHFRGNFPQKIAIDGLSTNEVIPTHDDSKWVSIIKPTKTTADKEHSFTELENIDQSYTHVKLTIIPDGGVKRVRVLGTIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUS4</accession>
    <name>A0A1E3NUS4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_18476</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesB/IscA family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036041.1">
        <property type="nucleotide sequence ID" value="XM_019180791.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUS4"/>
    <dbReference type="STRING" id="683960.A0A1E3NUS4"/>
    <dbReference type="GeneID" id="30198037"/>
    <dbReference type="OrthoDB" id="1938621at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044572">
        <property type="term" value="P:[4Fe-4S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.300.12:FF:000011">
        <property type="entry name" value="Iron-sulfur assembly protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="ATAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="ATAP_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00049">
        <property type="entry name" value="iron-sulfur cluster assembly accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43011">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43011:SF1">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core" evidence="2">
        <location>
            <begin position="2"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ56834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="118" mass="13272" checksum="0F52997FA5DADD78" modified="2017-01-18" version="1" fragment="single">LELDLTDRAVERLNQISKEENNPNVALRVNVESGGCHGFQYNLELSDISKKTEDDCIFERDNAKIMVDESSLSILRESKIDYTNELIGSMFKVVDSPYTTSSCGCGSSFDFDFSKLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUU7</accession>
    <name>A0A1E3NUU7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Chromatin modification-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_36727</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of an histone acetyltransferase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of an histone acetyltransferase complex. Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ING family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036071.1">
        <property type="nucleotide sequence ID" value="XM_019182463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUU7"/>
    <dbReference type="STRING" id="683960.A0A1E3NUU7"/>
    <dbReference type="GeneID" id="30199709"/>
    <dbReference type="OrthoDB" id="5411773at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140002">
        <property type="term" value="F:histone H3K4me3 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061188">
        <property type="term" value="P:negative regulation of rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061186">
        <property type="term" value="P:negative regulation of silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016479">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000219">
        <property type="term" value="P:positive regulation of invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17016">
        <property type="entry name" value="ING_Pho23p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15505">
        <property type="entry name" value="PHD_ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000016">
        <property type="entry name" value="Inhibitor of growth protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024610">
        <property type="entry name" value="ING_N_histone-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059153">
        <property type="entry name" value="NSD_PHD-1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZF_PHD_1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="249"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="137"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="204"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="9">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
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    <feature type="binding site" evidence="9">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="251"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ56864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="305" mass="34060" checksum="9A9C5679E66FAD3D" modified="2017-01-18" version="1">MRPTPKATEIFPGLNDITDAFEALPTDVIRYFTLLKEIDAKCVNVIPVLNHEIKKFIEMKNIPEHKEARTRKIHQICGLIRDVLPCLEEKMHVATIAADKTVTYIDRINDDFDLIFRNEVPEHIKFGSVNHPAIINESKLPDNKSAQSQRSESRREALAAKKAANDTDNDDSSTNSRAKSRREKEATPVATTTSTTATKKRKTTSTTANTGNSANISTTSNTAINTTGSKNDEVPSRPSTPSTNKRHEPLYCYCNQVSYGEMVGCDGDDCKREWFHLPCTGLATLPKGKWYCDDCSAKLKRAKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVQ1</accession>
    <name>A0A1E3NVQ1_WICAA</name>
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        <recommendedName>
            <fullName evidence="6">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">WICANDRAFT_86165</name>
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    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ57289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
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        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
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    <dbReference type="PANTHER" id="PTHR44051:SF8">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE GSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="16"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="109"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="26283" checksum="98D33CE39D3FAFA7" modified="2017-01-18" version="1">MVKVDEEDPKVGPIEAPYTKLYSVGTSNGQKITIFLELLKKEYIYRKIDLSTDEQKEPWFIKLNPNGRIPVLSDVDSEGKRITLHETGVILQYLADKYDKERKYSYDHDDPLWWEQLQWLTFQIASHSPMQGQAHHFVSFAKVVNDYGIKRYSDEAKRIYGVYEIRLKENNGWLVGDHLNIADISAYPWVSRSRMFKTDLSEWPHLAEWVSKISSIPGVSKGQSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVR5</accession>
    <name>A0A1E3NVR5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_65665</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035989.1">
        <property type="nucleotide sequence ID" value="XM_019183961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVR5"/>
    <dbReference type="STRING" id="683960.A0A1E3NVR5"/>
    <dbReference type="GeneID" id="30201207"/>
    <dbReference type="OrthoDB" id="10253125at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045903">
        <property type="term" value="P:positive regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000002">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000004">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005711">
        <property type="entry name" value="Ribosomal_uS5_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_uS5_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01020">
        <property type="entry name" value="uS5_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718:SF4">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="RIBOSOMAL S SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S5 DRBM" evidence="9">
        <location>
            <begin position="76"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ56782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="254" mass="27295" checksum="BE396CBFAB4FD1B9" modified="2017-01-18" version="1">MSAPTEAPKRGGFARNNRGRQGRRGGRRDGEEKGWVPVTKLGRLVKAGRISTIEEIFLHSLPVKEFQIIDTLLPELKDEVMKIKPVQKQTRAGQRTRFKAVVVIGDSNGHVGLGIKTAKEVATAIRAGIIIAKLSIIPIRRGYWGSNLGAPHSISAKTTGKSGSVSVRLIPAPRGKGIVASPAVKKLLQLAGVEDCYTSSSGSTRTLENTVKAAFVAIANTYGFLTPNLWKETELPVSPLDVFADEAASTKKAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVU5</accession>
    <name>A0A1E3NVU5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_65513</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036463.1">
        <property type="nucleotide sequence ID" value="XM_019183924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVU5"/>
    <dbReference type="GeneID" id="30201170"/>
    <dbReference type="OrthoDB" id="3980493at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF12">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="5"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ57256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="822" mass="96214" checksum="620DDE331EAFFB27" modified="2017-01-18" version="1">MRVVACISCRLRRRKCDRAKPVCTGCKDLNIPQELCVYKDLKAKTKSNDRKEILDQYKSDNMKLKNQKFRLMEGIELAFVEKSRLLREELASSSALNNESSPANVILQTQYKKSIEKDSKKNEQDITNNLHFGSISWGSLMTSEPDMQVLLEQMDSIIKRQKVQLDSLKKNFEEPVNENCILSKVKAYITSVALGIEKLDDFQIFQHLFHDIEKNLPTYKELKKFLDHQFRISQTDYVAFFSVDEETYYEILHRVLKFDGEGKPTIQISLPEQSSRLMDLAYVISYLIYIAYHRSQLIHDNHNANHMLLLEYSETLLHSYRLLCNAFKCDNSFLPTHTPEELHTMLYITTFERYTPHGRHKSNESIDNTMNSRNLTSIARVLQLNQNIDIVYSGRSENYRKSLKSAWYLLVLVDVMESLESGLPPKIRPDEILRYKDHYNGYIESLIVLNRVLYKFHKYDLDSMQNTYEFVRIVDEELVTDLRRLLKSELRPARYDFECLLGFDFDDISMSGEFVKETACFAIKFNIFSLIQTLYFICFKKLEMIDKESDETKKFNILSMKYSTLIISLIGEVFILYDKLSSHPNSYEYAVLESIMAIFPHLSLALRRVYICAGARIFEKLPINKPSAVKRFLYARPANENTLLKELHLKQEYQIDYKIEDLADYDADEDINELFARFSPLLDYRFVIFNFSQVLKKISENLFNEKSNINFVKLNHIFFYLLKLTSFFLNATFVDDAKALGNATSFKGLHDTPRASRYSDESESESEFNFKDLFEKAINTKSENFDFKEFFNNTNGIYQTTDIDDFLTTYPMSVLGGSNQEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW14</accession>
    <name>A0A1E3NW14_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SUN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_106891</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036568.1">
        <property type="nucleotide sequence ID" value="XM_019180484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW14"/>
    <dbReference type="STRING" id="683960.A0A1E3NW14"/>
    <dbReference type="GeneID" id="30197730"/>
    <dbReference type="OrthoDB" id="4065610at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034993">
        <property type="term" value="C:meiotic nuclear membrane microtubule tethering complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045119">
        <property type="entry name" value="SUN1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911:SF8">
        <property type="entry name" value="KLAROID PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911">
        <property type="entry name" value="SAD1/UNC-84-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="SUN" evidence="8">
        <location>
            <begin position="342"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="559" mass="64814" checksum="B08603A34909FFEB" modified="2017-01-18" version="1">MSKSFQFSTDALTNDDDVGEDIESEYDINPSLLNAKYSGFKKQYEEEEEEEEEESDNDYDDSTIDDVKETMYTGDINAIDEGDDDVNYYLKSTLRIIYKVKQNWIKILLYFLLFITNLGIISYFNSNNGGSGVGDHNDIINIRNFGDLTKNIQHLQYQINEINQKQKTKLHEFKNYLDLKLDEFSLNFKKIDKDIEDLKRENNKILDRVNKINIGDIEISNDKLPILLDEHNNIKVIPEFSSFLQKKIQDIIDGEINGSKFKLNYKSFIENHVEDIINSKVGFMNKEEILSLITLQFQENKKKLINEIKSLTRVNNIPQKLVETVKVKKPNGKINYGQAISGARIINYLTSKTFKPRFKKGLWDILGLNSNHDDSNEVEIYENTSPFIVLTSDEGYWRSSQIENTQLSIKFLEPIYMNEFQYCHGDVLNGGIITSCPKHLSLYVQMEGGEAMGDEEIQGHFKISEMVYDVNKSEQEEQGFEIPEEVQRKLVKSVIIVAKDNYGNEFYTSFHKFKVYGMTKFDLYSVKKILNNDEIDDAIKRQYQANGVKSFGDDRVVYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW47</accession>
    <name>A0A1E3NW47_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_71435</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036119.1">
        <property type="nucleotide sequence ID" value="XM_019184244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW47"/>
    <dbReference type="STRING" id="683960.A0A1E3NW47"/>
    <dbReference type="GeneID" id="30201490"/>
    <dbReference type="OrthoDB" id="9974981at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051609">
        <property type="entry name" value="NmrA/Isoflavone_reductase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47706:SF9">
        <property type="entry name" value="NMRA-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47706">
        <property type="entry name" value="NMRA-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ56912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="30355" checksum="0FB365F79F337A7A" modified="2017-01-18" version="1">MSTVAIIKTPIRLLTSSDKRRIQSSKVKYYNVKEVGFDKALEGVDVVINLGGVPGDPKDDILLKSIISSNIKLYIPSQYGTDLYVSDTLPNFLGTKTAHSIAARDGGVKTVDLFTSVFINEDGLLPYAPLYLFGDVENTKEVTIPGNENTLVNPTYVKDIANAIAALVTKDDYASIPDKVRIYSDRVLATDLVKHYEKIKGVKINTRFVSTAEQVKKAIEKYSHGFRLEDIGFYLATILSEGEGKGLIYEKENEKEFVNPNETLFKWTRFQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWF8</accession>
    <name>A0A1E3NWF8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_98149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036114.1">
        <property type="nucleotide sequence ID" value="XM_019186635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWF8"/>
    <dbReference type="GeneID" id="30203881"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="67"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="256"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="280"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="334"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ56907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="414" mass="42899" checksum="5108A988F412C46D" modified="2017-01-18" version="1">MSVSHSSKLLDSTSDHTTSFRSSGKSTVLHPSNLVSSFISTLEGTSSTKTPSETALTRSDLSLTEHSTVSSVKDDITSSTTIDSDTSSEEQQQTSLVSNTDSKTAPTDTSSLPAGSTLSSSTDPTSNSFSTANTVTTNPENTVQTTSIDFNGTTDTSEDDTPSIGEPTLVSTVQTTTTFGSVTNIIPNRSDVVSSGEAQINTLPTGSVISSELNGSTGIPSTASGSEQDGSSTSESQTDLTTMISTSSSPNLGNNDSVTSSTDLLSPTPTHTNESEKSESTTVSESTVSSLPPAERSSSNLDDWTSTDTNPIMSVAVASTKTNPVDESTSQDFNSSTNTPTITNIQSSSTGTIRDSQSSSEHNWMSTIVMDGSTTAIYGSGSSTTAVISYSEGEGVTLSINLKMTIFSIILFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY02</accession>
    <name>A0A1E3NY02_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_95424</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037296.1">
        <property type="nucleotide sequence ID" value="XM_019186493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY02"/>
    <dbReference type="STRING" id="683960.A0A1E3NY02"/>
    <dbReference type="GeneID" id="30203739"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000064">
        <property type="entry name" value="MFS allantoate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF1">
        <property type="entry name" value="ALLANTOATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="441" mass="49286" checksum="41D206786A5D4925" modified="2017-01-18" version="1" fragment="single">MADKDSKSIEVKDGVVATDSNGNSNGEVTEFNDLPVNAKVAKEVDRGFAMAQETEGIELDPIKEKALVKKIDWYILSTMCALMSCQLMGKSSNSYASIMGLREDLNMTAKEYSWVGSSFYLGYLFFEYPAGLLLQRFPMSKVLGVAIVLWGVVLCCHGACNSSVTFLLCRTLLGIMQSFMDPAYMTMTAQWYRKEEQYIRCAYWLGLQGFGTMLGSGIAYGFYTHQDSISIRPWTGLYIVVGLITVVFGIISFIHVPDIPTKAWFLNEEEKSYVVERIRQNRTGFGNPKFKVSQVKEALLDPCIYLFFLYMFGYGYTNGALGNYGSIIVKDYLGFSTGQSLLLNMVGSGQDIIFPLFWAYVNKYLIKSRLIVAFIINAIVWSGLFMLAFSENRGAKAYGYFMNYWLTASWATLSSIVQTNVAGHTKKSVANSLFLVGFSVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY81</accession>
    <name>A0A1E3NY81_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_34302</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3B4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037376.1">
        <property type="nucleotide sequence ID" value="XM_019182170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY81"/>
    <dbReference type="STRING" id="683960.A0A1E3NY81"/>
    <dbReference type="GeneID" id="30199416"/>
    <dbReference type="OrthoDB" id="10259687at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048026">
        <property type="term" value="P:positive regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12334">
        <property type="entry name" value="RRM1_SF3B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000505">
        <property type="entry name" value="Splicing factor 3B subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034158">
        <property type="entry name" value="SF3B4_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052084">
        <property type="entry name" value="SF3B4_spliceosome_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030:SF3">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="14"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="104"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="210" mass="24279" checksum="D11E0FA7AA72BCC7" modified="2017-01-18" version="1">MLKRGIESDRNQDCTVYVGNLDKQVNEGLLYELFLQIAPIKNIHLPKDRILRTHQGYGFVEFKNVKDVEYAEKVMSGTKLFGKNIKVNKVGNNAANNDNLDIGSTLFIKNLDELVDENLLFTIFKQFGNFFKNPIVVRDDQGNSKKHGFIYYKNFKDSDNALSKMNNQFILNKRVQIDYAHKKKNGKLVKHGDETERLLLTEAEKNNYNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY97</accession>
    <name>A0A1E3NY97_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_80234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037280.1">
        <property type="nucleotide sequence ID" value="XM_019185025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY97"/>
    <dbReference type="STRING" id="683960.A0A1E3NY97"/>
    <dbReference type="GeneID" id="30202271"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="26328" checksum="7DE5A0DA87DE81F7" modified="2017-01-18" version="1">MLKFSDINDFLMDYNELLIGNYPIHSQAPGCPKNLVCSYSGPGWVETENITSKGMIYVLICRSGYSMYGIPVAQVTLVDFYGHLYVKENFQLEEQYLEFGSDDDGSDATLSEDQLFHIQQELLKVISTKDIIVGFALDRAFKNLKLKHPNIIDIAHLYYVFFMDDSKTESQYLLFLAQMFIPHGYRSFLTGSLSDFQEDSKICWMLLVLRLLSKNKCLKALEYQKQGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYA4</accession>
    <name>A0A1E3NYA4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_69960</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037177.1">
        <property type="nucleotide sequence ID" value="XM_019184187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYA4"/>
    <dbReference type="STRING" id="683960.A0A1E3NYA4"/>
    <dbReference type="GeneID" id="30201433"/>
    <dbReference type="OrthoDB" id="4159781at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF12">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="25"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ57970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="825" mass="95103" checksum="4672ED806203D736" modified="2017-01-18" version="1">MSNPIHMPEFDRPAPVTKRARVTKSCTICRQKKIKCDKVKPTCGACAKRGRSGECKYEEPDWNINVMNNGGSINNNSSTHNGETVDEQLILLRDKLKRIESVIKKDNLYTTEPTFINNTVEPEIEEDGVIDFYKHNYGSPRMFTQGVLSWVTLLKKDVYLRSVVESSRRKKIIVTKSGRKEDTKETEFIKKFKVEQDFDAEISDTTSGQICKYKNKDILMMIKSTLVDSKLIWLLVEKFFDSELYLVLPLFDKAEFTSQLYDILGSKSSQRVILNIRKKLQLNTIGILLIIMRLTSLSTYNALRSNTENLSENDLYILNNPVTKEYMTAAHRCYDEISQFREAHLEIFQFQLLSRYYDQLAPEDSDCNSMKTYENMGPLVHSAVSSGLNRDPSVADPGNENPNLLRRLWFKVVNLDYNQLMHIGCPPMINVRFYDTLYPSLNPYDSPTEYAINQAIHKRKDIYEMCHPLLMLLLNVRDPPRLSTVKQHLRPLENLVQAHPKVSDIMKMPSDTVLQRFEKLTSVAGLVDTTSLLYMIYYHIYLHYNERGVKDKSLYYLLEIIKIMTGAFPLINFLLEETEYNSDYNLKNHFGIPILVIPEIELLSHRSIQVMFSLLARIKAMKVFTIDLDPAKLELVDNIADSLLRSASYILKGFLNIADTYYHSWAMSRIHSFILKDILYNKRGPYNPENASDADRAFIKNLNLVRDHDELFMKYSIDDLKQIYESVKELERCGTGSERPSPKGSVDMTFSSAANQHDSLWFNQFQSDTGLLYDNKGHMGGSSSASSGPTISDPILDRMDDPVINNFDIMDIERFFFHSTNESMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYJ3</accession>
    <name>A0A1E3NYJ3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_33743</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037478.1">
        <property type="nucleotide sequence ID" value="XM_019182110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYJ3"/>
    <dbReference type="STRING" id="683960.A0A1E3NYJ3"/>
    <dbReference type="GeneID" id="30199356"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="21"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="4">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="313" mass="35406" checksum="E117A8BF15C6F256" modified="2017-01-18" version="1">MTEKATDIQFTFYNGQKIPALGFGTANVGDRAPPTKEALKIAVKAGYRHIDTAWVYQTEDYIGEALKELFEEGVVKREDLFITTKVGTPLYQDPERSLNESLKKLGIDYVDLLLQHWPYGSEPIFDKDGQLDRTKSIESKDALNKDYIKSYQRIEKLYKEHPEKVKSIGVSNYSVEFLENLLKVAEVKPVNNQIELHPHLPQKDVVEFSEKNGILLTAYSPVGSSGAPNLEIPIVKELAAKYNVTANSILTSYHIKEGRIVIPKSINEQRIKDAIVLAPLTEEDLQKLSEFGEKNPKRFIAPAWGKDLGFKVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYR7</accession>
    <name>A0A1E3NYR7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cns1/TTC4 wheel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_97143</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036934.1">
        <property type="nucleotide sequence ID" value="XM_019186567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYR7"/>
    <dbReference type="STRING" id="683960.A0A1E3NYR7"/>
    <dbReference type="GeneID" id="30203813"/>
    <dbReference type="OrthoDB" id="420195at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21381">
        <property type="entry name" value="CTWD_TTC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044059">
        <property type="entry name" value="Csn1/TTC4_wheel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46035">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46035:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18972">
        <property type="entry name" value="Wheel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Cns1/TTC4 wheel" evidence="4">
        <location>
            <begin position="96"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="31"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="227" mass="26006" checksum="64B0737A7C0A75C8" modified="2017-01-18" version="1">MAKAFFTIDKIDEALSALEFSLGLDPTNAASKALMTKIQNKKHELETIEAKKEAERLHKEILQDKLNTAIKGRNYTIIKTPRPAEMLTDAKLSLEDPEDIESQLIFPAMIMYPTTDEFDFVASVSELTTPWELMELVLQRPQEWFQDPKHENFTPKKLDAYMETESGGLIKVGKKASLHKALSTEKPKIPLFDNALRIYLVPRIDAENWLSTWDKEITLKKRTELSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYY5</accession>
    <name>A0A1E3NYY5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Stretch-activated cation channel Mid1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_34516</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037461.1">
        <property type="nucleotide sequence ID" value="XM_019182197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYY5"/>
    <dbReference type="STRING" id="683960.A0A1E3NYY5"/>
    <dbReference type="GeneID" id="30199443"/>
    <dbReference type="OrthoDB" id="5405745at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024338">
        <property type="entry name" value="MID1/Yam8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39142:SF1">
        <property type="entry name" value="AEL197CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39142">
        <property type="entry name" value="MID1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12929">
        <property type="entry name" value="Mid1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133573" description="Stretch-activated cation channel Mid1" evidence="1">
        <location>
            <begin position="23"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="565" mass="64237" checksum="ACD003104AE9BC80" modified="2017-01-18" version="1">MIKYQLLFALIVSLCLSGTVVAISNHDELIWDIWNNEEGEPFDNNQISCSSIEDLNTRELCYRNSGEVQSKEEGSFSLEKRKIKTSNVVHDIYNEAPISIQLEPGSRINYRYLINMSDPAAQQFYEVMVFITGNLCNFPQGLSDPNSYLSLYTAFNDTGIFTNSSDSPFYSQTYNQKDFQYGYLQSLSDTNVKTNESFYLNIVLEADEIEDVNGTFTIQLGLSQDDLIYQWDNRTWINLVDTDDTTALFMTGNLTTAYNNATDILDSSSNYYTVHIFLDEDDNYFSNLNRSWCSIVNGNELVKQTNISYSFTQRGGGLKEQMYVKGLQPSTDYIAYVTQDFSSTQHGGVVFNRLQFSTQEPDTCKLVFNLTFCDNVAYSVPRSNNVQLDSNFQELADLYDSNVKDMFGNFSKALQQIPCQAEDDQRYSPIVTCDDCATSYKNWLCAVSIPRCTTNNETEYKYRELNESRNDYINSKVDPPSPYYEILPCLSMCHAIVRDCPTDFGFACPRDNTSIGKSYWFMSDDSTYDTCNYVGALSTSGALPTIGMNRIGLFISFMVVSLVMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ10</accession>
    <name>A0A1E3NZ10_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glycosyltransferase family 15 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_64598</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037668.1">
        <property type="nucleotide sequence ID" value="XM_019183767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ10"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ10"/>
    <dbReference type="GeneID" id="30201013"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF6">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="405" mass="48662" checksum="9F62993AAC4F8F98" modified="2017-01-18" version="1">MSRFNRLLRFTVVLILIIFFISHKIISKKQYQHVLDLQSKLANTKYDINSQLNIHDSASQEKIIYKDSTNDLKNYQRANATFFTLAKNEDLIEMGKTIRSVEDRFNKKFHYDWIFFNNQEFSPDFKRTMNNLCSGNVKFEIIPQEFWSYPDWIDKERAKQVRISSRTDIIYGGSETYRFMCRFFSGFFYKLESLQKYDYAWRVEPETTFHCDINYDVFQYMVSNNKHYGFTISLHEFANTIPTLWENVKKFIEFYPHFINPNNLIDFLSDDHGETYNLCHFWTNFEIVNLNFLRSEAYETFFNYLDKANGFFYERWGDAPVHSIAVALFLNKEQIHYFEDIGYYHKPASNCPINDDVWKENNCLCDQWMDVTFRPFSCTPKFYDLLKLKKPDNWKEHAAMAQLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ26</accession>
    <name>A0A1E3NZ26_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_96069</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037600.1">
        <property type="nucleotide sequence ID" value="XM_019186511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ26"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ26"/>
    <dbReference type="GeneID" id="30203757"/>
    <dbReference type="OrthoDB" id="427480at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14655">
        <property type="entry name" value="ZIP_Hap1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF90">
        <property type="entry name" value="CENTROMERE DNA-BINDING PROTEIN COMPLEX CBF3 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00755">
        <property type="entry name" value="AFLATOXINBRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="47"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="135"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="405" mass="46014" checksum="BCDB60A19DEFD5FE" modified="2017-01-18" version="1">MSSLPNSNNKRAVSQEANGSPNSSAAGAKEAKDKADRLAKRQKITLSCTECRRRKIKCDHKKPVCENCIKKNLQEQCTYSTSPWIQIISSENQIYEEIESLKRENDLLMKNIALLKEQCGKLNVDSNSLLSSNSAGELSSGSTASPISNSQLTPSSTTSSAQSTAQSKEDTIAKQLSNLLLKSIVGRIRSQDVQLINIPPKYNSPLTPMLTLKINNDKNYKSIFLNQLLINYLPNFQMIEYHINYFFKSNFHKNLQIISQSQIMETFHRIFNQHSNKIEIDSKREIQDFLQLAIILTILKITSVYKDTPFNDPENKLILYIDAALGFGELITRSSIPALQAVILLNSYRLFVEDMNEIDLYFLIQLCQDFAIAIGLHKNIDVLYEDKSVEEREMLKTIWNCLNDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ67</accession>
    <name>A0A1E3NZ67_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_80641</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NSE2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037713.1">
        <property type="nucleotide sequence ID" value="XM_019185102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ67"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ67"/>
    <dbReference type="GeneID" id="30202348"/>
    <dbReference type="OrthoDB" id="756301at2759"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16651">
        <property type="entry name" value="SPL-RING_NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054753">
        <property type="entry name" value="MMS21_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026846">
        <property type="entry name" value="Nse2(Mms21)"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21330">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21330:SF1">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22326">
        <property type="entry name" value="MMS21_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11789">
        <property type="entry name" value="zf-Nse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="E3 SUMO-protein ligase MMS21 N-terminal" evidence="11">
        <location>
            <begin position="71"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="SP-RING-type" evidence="10">
        <location>
            <begin position="178"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF11789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ58506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="273" mass="31157" checksum="1792BBC8C86565A2" modified="2017-01-18" version="1">MSEGLQLPSYFPVSRSLQRELEDLRDSSTSLDELKKTQEEILKAAVTYFQLLFPDDANSEWNIEGAVNLELIKQLLDSYESLVGAQQQTTHFNQTFNKAKNQIRARLREEPELSVDNIDAYRSVGTNKKSFGEILDEELKRVPPINKDESNPDYIFLKNATFVISHPLDPLPDEEEDDDIQVGGGKIDLKCPISMNYFDAPMISTKCKHTFDQASILGTWKSAGVMEDCPTPGCRARLRRADFIPDRLMTLRVKSFKAQEKKLENAEEYEKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZJ7</accession>
    <name>A0A1E3NZJ7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF1445 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_85173</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the D-glutamate cyclase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037234.1">
        <property type="nucleotide sequence ID" value="XM_019185855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZJ7"/>
    <dbReference type="STRING" id="683960.A0A1E3NZJ7"/>
    <dbReference type="GeneID" id="30203101"/>
    <dbReference type="OrthoDB" id="10262538at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047820">
        <property type="term" value="F:D-glutamate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006536">
        <property type="term" value="P:glutamate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2040.10:FF:000001">
        <property type="entry name" value="D-glutamate cyclase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1640.10">
        <property type="entry name" value="PSTPO5379-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2040.10">
        <property type="entry name" value="PSTPO5379-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009906">
        <property type="entry name" value="D-Glu_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038021">
        <property type="entry name" value="Putative_hydro-lyase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32022">
        <property type="entry name" value="D-GLUTAMATE CYCLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32022:SF10">
        <property type="entry name" value="D-GLUTAMATE CYCLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07286">
        <property type="entry name" value="D-Glu_cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160920">
        <property type="entry name" value="PSTPO5379-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="29967" checksum="D10728EF548BCD06" modified="2017-01-18" version="1">MTIQRPEDFRSSCRAGKFNTQTSGQCPGFAQANLLVIPINAAQDFIELCERNPVPCPLLAITKPGDPTVFTKGSSIFKTTTDLSTDFPKYNVYDNGKLISTQSDISKEWSKDHVGFLVGCSFSFEFALSKAGLTPRNMSQGFTVPMYHTKKYLDPAGVFTNSTYVVSMRPYKLKDIPKVREITKKFKPTHGEPIDWGFDALERLGINSLEDTIFSDPIELEEGEVPVFWGCGVTPQLAAAEASDKVKGTIMAHAPGHMIVIDVKDEEVPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P090</accession>
    <name>A0A1E3P090_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_15953</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037891.1">
        <property type="nucleotide sequence ID" value="XM_019180667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P090"/>
    <dbReference type="STRING" id="683960.A0A1E3P090"/>
    <dbReference type="GeneID" id="30197913"/>
    <dbReference type="OrthoDB" id="3364175at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF6">
        <property type="entry name" value="PROLINE UTILIZATION TRANS-ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="6">
        <location>
            <begin position="231"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="524" mass="60456" checksum="A9BF0C799B2B063F" modified="2017-01-18" version="1" fragment="single">FLPPSLQPLFHFPLSIQPNEEEEDNDETKKNNDMTLVPEEEGLNSRLLYDTGGNLRYIGESSAGSFLPQCRKVFQKILGISKFTHDPQRFLMIDSPATRGMRVPVNLPPRDYANHLIQLFKKYINNVSYVFNEEYFEKTLIETVFNNPLNATPKQLCLVYLILAIGSIFAKIELQRSIFPFAINYFVEPHVFFESAQHLLQSTLNDSNLWLVETYYLMYFYDELNMTRNTSWINLGTAIRYAQGLGMQRRYVNESFKDPEYTLHRRKLFRSLYIADRLCAIHLGRSLTINDVEWDDKNSLNQFNDLQNEMLKVCLLNGKILTKVYHNPAIGMKNALKLAIELKIFTVNSSISKSLSLQNTLKYNDNHGLLWLHINHLDGIILLARPFFFFIILIKLKFIKFDEESKNYSNLRNFYQSCIKASLLTIKLIEFYFVNNLHPLKGYTIISCSFHAGLIIGMILLLQSQNIDDNLFDSKANVNGGESSKVVCISAMNSTISVLRHYGAIDPTSKRYGEILSNMMDATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0E8</accession>
    <name>A0A1E3P0E8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA-processing protein 7 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_79474</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRP7 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038136.1">
        <property type="nucleotide sequence ID" value="XM_019184900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0E8"/>
    <dbReference type="STRING" id="683960.A0A1E3P0E8"/>
    <dbReference type="GeneID" id="30202146"/>
    <dbReference type="OrthoDB" id="5390at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12293">
        <property type="entry name" value="dRRM_Rrp7p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040447">
        <property type="entry name" value="RRM_Rrp7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040446">
        <property type="entry name" value="RRP7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024326">
        <property type="entry name" value="RRP7_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13191">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13191:SF0">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 7 HOMOLOG A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17799">
        <property type="entry name" value="RRM_Rrp7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12923">
        <property type="entry name" value="RRP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rrp7 RRM-like N-terminal" evidence="3">
        <location>
            <begin position="7"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA-processing protein 7 C-terminal" evidence="2">
        <location>
            <begin position="171"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="34053" checksum="FADBF56AA9A7BDF4" modified="2017-01-18" version="1">MSEAIEEIKGFQVVPLLLQPSKLLKNQSIKHYIYVKKHQSNTDKELAERSLFLINPPINSTLSNIKNFFKKISQSSIIENFFLNVENLDYEINLTKLTTDLYDQDEKNLQEGIKLPNGTGLVVFVDKAAANLAFSKIKKYVKNSHKDLYQWNFNEDQPASIRFTKAYQNDILPAEETSEAVAQALEDFEKREADSISQLEEMKTLVDEDGFTMVVGKNRKTKKGILGKIDSVNKVVKDKNEVKSKKKEKEDFYRFQIRERKKLEMNELVKKFRQDQERIKELKEKKRFRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0H9</accession>
    <name>A0A1E3P0H9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein PXR1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">PinX1-related protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Protein pxr1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_33501</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PINX1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037614.1">
        <property type="nucleotide sequence ID" value="XM_019182078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0H9"/>
    <dbReference type="STRING" id="683960.A0A1E3P0H9"/>
    <dbReference type="GeneID" id="30199324"/>
    <dbReference type="OrthoDB" id="29523at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010521">
        <property type="term" value="F:telomerase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032211">
        <property type="term" value="P:negative regulation of telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050656">
        <property type="entry name" value="PINX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149:SF31">
        <property type="entry name" value="PROTEIN PXR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="G-patch" evidence="10">
        <location>
            <begin position="25"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="143"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="198"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="267" mass="30833" checksum="7346E6F1477371D5" modified="2017-01-18" version="1">MGLAGTKVKQRFGMDPRNTKWANNTDRFGHQYMEKLGWKPGSGLGLVSHAMTTHVKVAIKDDTVGLGAKLSKKNKKDEFDSGECAGLDVFQRILGRLNGREDEISKELDIQKRENIINGKWGMSFVKGDVLKSTWDAESKKLIASKKRSRDDDEDDKEDEEKKEKKDKKEKKEKKEKKEKKEKKEKKEKKDKDEEKKDKKKDKKDKKDNKHKKESSEEAVTRDSMLAPSSREDTPVGLRHIRSRWIKQKRAATLDSKALNEIFMITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0P8</accession>
    <name>A0A1E3P0P8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SWIRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_80622</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037694.1">
        <property type="nucleotide sequence ID" value="XM_019185096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0P8"/>
    <dbReference type="STRING" id="683960.A0A1E3P0P8"/>
    <dbReference type="GeneID" id="30202342"/>
    <dbReference type="OrthoDB" id="5598695at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="35239" checksum="31D41BCA2C6904FF" modified="2017-01-18" version="1">MTDREADSPQRRAKLLSPPISPEGKLAAQKRRLDETEASSTQAKDSEWMEIKPQPAKKKQIMNFLGAYNLPQLKKNSTKSKPSKKSPPAPSSSSSSSSSSTPSDKSEESKKEEELNIEEASVDGSQKASKNSTENVASREYDTRSKKKTSPSTSTPSSLSSSASTSEANTDEVTDLKEKLVEAKNNGEKGEFLRLSTVSLIHDLPLEDIPNFEPKIELNDEEFLKIIKKLGLTYKLEDGETKETTIKNVLKLTDQQFIETNKRFFAEKARRTYNEQTFKKTDAQRACKIDVNKTSKLYEIYQACDALDDDLFRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P108</accession>
    <name>A0A1E3P108_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_32336</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Cooperates for the ATP-dependent vacuolar transport of bilirubin and glutathione conjugates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038220.1">
        <property type="nucleotide sequence ID" value="XM_019181945.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P108"/>
    <dbReference type="GeneID" id="30199191"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18603">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000020">
        <property type="entry name" value="ABC metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000001">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000997">
        <property type="entry name" value="Multidrug resistance-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056227">
        <property type="entry name" value="TMD0_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF443">
        <property type="entry name" value="MULTIDRUG-RESISTANCE LIKE PROTEIN 1, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24357">
        <property type="entry name" value="TMD0_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="374"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="476"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="890"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="931"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1021"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1119"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1145"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="258"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="590"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="895"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1210"/>
            <end position="1451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ59013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1460" mass="162986" checksum="014827A24F324611" modified="2017-01-18" version="1">MSVFLDQCPYGWTLLIAGENAINPCVFEYLMLSNVVIMMPFGFYQLWSTMQKPAPGKIKYNKIGALGLLRVIATLLQAVLLLSQKDQLYFFANGLIILFVTLPLTFFALFKNPVASGSVIFYWLFQMVVYAIAACQNILTVYKVADDNPVIMYVVLPNAIHLFVSNAFLYTPVHEVVKESLESGLLHANILSKLTFFWMNDLIKKGYNNEQITDEDLPIPPAQLDPGKSYNDLKKVWENQENKSLLFALSKVFGFTVVTAVFYAVLDDILSFAEPQLLRLLIKFFDEDQPLIVGFLIAFGMFFVSVLETALFNRFFISIYEAGLGTKSALMTLIYNKALKLSPEARKERSTGDIINLMSVDVNRIQDLTNQFQMMISAPVKLVLCLVSLYALLGKSTFAGIITMAIAIPLNTLVSRRLKGLHKTQMKYKDERTRTTSEMLSSIKSIKLYAIETAILEKLDYVRNVLELGNLKRIRIFSAFMVFSWNCVPFFVSCSTFTAFALIENRPLSPEIVFPALSLFNLLADPVYEIPSIITALIETSVAFQRITSFLLGEELDEELINYYEKVDAAGKTTVKISNATFLWENPPQLKKKDYDEESAVSESKVALKIDSFVAKKGELTCIVGRVGAGKSTFLQSILGQLPCVGADNNNKPEVDIHGSIAYCAQVPWILNASVKDNILFGHKYDDKYYHKTIECCQLLPDLDILPDGDETQVGEKGISLSGGQKARLSLARAVYARADIYLLDDILSAVDAHVGKNIISNVINGLLSTKTIILSTNSITVLNHANNILLLADGKIVEAGSFKDVMGSGSKLSTLMTEFGAKFDDDANDEDELPLERTKTTETVRRASIASLKIRPANKKKQRTAQEEEKSATGKVSFAVYKAYAKACGIPGIIVFLAFLMSAVLFSIIGNYSLKQWSESNRKNGSNKDVFKYVGIYTFFGIGSGFLTLGRTITMGVFCGLRGSVVLHNKMSRAVVRSPMSFFETTPIGRVLNRFSSDINKVDEGLPGVFSFFFTSCAKVFFTLGFIGITMPSFLLVIAVLSVLYIYYQRYYVGTSRDLKRIVNVSRSPIFAHLQESLSGYETIRAYQQQPRFQFLHLTNLESNLKSLYIFRSINRWLSVRLQFIGSIIIFTTASLAITNHLSSGMAGLLISFALQITGSLNWIVRMTVEVETNIVSVERIMEYCELKPEAEEIIEPRPPKVWPSHGSVKFENISAKYRENLDLVLKNVSLDIKPREKVGIVGRTGAGKSSLMSTIFRLIEPTDGKIFIDGLDIGKIGLHDLRSNLAIIPQDSQSFEGTVRQNLDPLELHTDEELWRVLDLSHLKSFIQNLDKDNEDGHVGLEAKLSEGGSNLSVGQRQLLSLARALLNPSKVLILDEATASVDVETDQIVQKTIREAFKDRTILTIAHRIDTVLDSDKIAVLDQGQVKEFDTPDNLLANKESMFYQLCSQGGYIKEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P117</accession>
    <name>A0A1E3P117_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">anthranilate synthase</fullName>
            <ecNumber evidence="3">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_84747</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the anthranilate synthase component I family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="3"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038302.1">
        <property type="nucleotide sequence ID" value="XM_019185769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P117"/>
    <dbReference type="STRING" id="683960.A0A1E3P117"/>
    <dbReference type="GeneID" id="30203015"/>
    <dbReference type="OrthoDB" id="1865897at2759"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005950">
        <property type="term" value="C:anthranilate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005256">
        <property type="entry name" value="Anth_synth_I_PabB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00564">
        <property type="entry name" value="trpE_most"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="AMINOBENZOATE/ANTHRANILATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236:SF9">
        <property type="entry name" value="ANTHRANILATE SYNTHASE COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Anthranilate synthase component I N-terminal" evidence="9">
        <location>
            <begin position="60"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="8">
        <location>
            <begin position="238"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="517" mass="58362" checksum="48B1BE9BB43A31B6" modified="2017-01-18" version="1">MQSPIQPSLDSLISIIQKDREELPTDKIPNLYPIYSLVSNDSITAHIAYLKLTKLGDETHKKTPSFLLESAKNGDTVDRYSFIGFNPKKIINTGPQFNKEVDPLDILQEEMSQYRQAQLPGLPRLSGGAMGYISYDCIRYFEPKTKRDLKDVLKLPEASLMLFDIVIAFDNVFQRFQIINNISIELDDSLDLIKSKYESAKSQINEIETILFDDSIPIPYPKQSPIVENQTFTSNIGQEGYENHVKTLKNHILKGDIIQAVPSQRVARPTSLHPFNIYRHLRTVNPSPYMFYIDYLDFQIVGASPELLVKSDSKDKVITHPIAGTIRRGKTTEEDDELANVLKSSLKDRAEHVMLVDLARNDINRICDPNTTNVDRLLTVERFSHVMHLTSQVSGTLRSDKTRFDAFRSIFPAGTVSGAPKVRAMELIGELEQEKRGVYAGAVGSWSYDGKTMDTCIALRTMVVKDGVAYLQAGGGIVFDSDPYDEYIETMNKMKANNNTIVEAEKIWIEKLAKGEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P159</accession>
    <name>A0A1E3P159_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_84660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038197.1">
        <property type="nucleotide sequence ID" value="XM_019185752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P159"/>
    <dbReference type="GeneID" id="30202998"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="53" mass="6333" checksum="45DECAFB7B78C2AA" modified="2017-01-18" version="1">MAFHVTGFGTSAIFFRHCRENRQKPAGRRWKEVCLKLNRELVETRTELLAWEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1F4</accession>
    <name>A0A1E3P1F4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PUA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_84488</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glutamate 5-kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037949.1">
        <property type="nucleotide sequence ID" value="XM_019185717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1F4"/>
    <dbReference type="STRING" id="683960.A0A1E3P1F4"/>
    <dbReference type="GeneID" id="30202963"/>
    <dbReference type="OrthoDB" id="409889at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004349">
        <property type="term" value="F:glutamate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901607">
        <property type="term" value="P:alpha-amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04242">
        <property type="entry name" value="AAK_G5K_ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21157">
        <property type="entry name" value="PUA_G5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.10:FF:000008">
        <property type="entry name" value="Glutamate 5-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000020">
        <property type="entry name" value="Glutamate 5-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00456">
        <property type="entry name" value="ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041739">
        <property type="entry name" value="G5K_ProB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011529">
        <property type="entry name" value="Glu_5kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005715">
        <property type="entry name" value="Glu_5kinase/COase_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019797">
        <property type="entry name" value="Glutamate_5-kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01027">
        <property type="entry name" value="proB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654">
        <property type="entry name" value="GLUTAMATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654:SF3">
        <property type="entry name" value="GLUTAMATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000729">
        <property type="entry name" value="GK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00902">
        <property type="entry name" value="GLUTAMATE_5_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PUA" evidence="10">
        <location>
            <begin position="312"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="434" mass="47685" checksum="23DDEEE7ACE7D701" modified="2017-01-18" version="1">MAKSKSYTIVIKLGTSSLVDEKTKEPRLSTMSLIVETVVKLRRAGHKVVIVSSGGIAVGLKTMGLDKRPKKLSQVQALAAIGQSKLIGRWDSLFGQLNQRIAQILITRNDISDWTQYKNAQNTMNELLSMGVVPIVNENDTLSVAEIKFGDNDTLSAITAALINADYLILMTDVDCLYTDNPRSNPEAKPILVVEDINELKVNTSSGGSDVGTGGMTTKLIAAELATEAGTTTIIMKSSQPENMWKIVQHIQDHDVDYKDEDGNALELDDVQTGELKELKLNDVPLHTRFIGKDKHVKNREFWMLHGLQTHGTIIIDKGAHSALTRSNKAGLLPVGIIDVVGTFHDHECVSIKVGSRLPNGELDTKEPLLEIGRARVNYSSTEIEKIKGAHSQQIEEILGYADSEYVAHRDNLAFPPSVLESTQSHEHMVPNLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1F7</accession>
    <name>A0A1E3P1F7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_19729</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037954.1">
        <property type="nucleotide sequence ID" value="XM_019180838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1F7"/>
    <dbReference type="STRING" id="683960.A0A1E3P1F7"/>
    <dbReference type="GeneID" id="30198084"/>
    <dbReference type="OrthoDB" id="1684102at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015186">
        <property type="term" value="F:L-glutamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015188">
        <property type="term" value="F:L-isoleucine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032974">
        <property type="term" value="P:amino acid transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015824">
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        <property type="project" value="EnsemblFungi"/>
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        <property type="entry name" value="AA_transpt_TM"/>
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    </dbReference>
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    </dbReference>
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            <end position="189"/>
        </location>
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            <end position="215"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="269"/>
        </location>
    </feature>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="403"/>
        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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            <begin position="492"/>
            <end position="509"/>
        </location>
    </feature>
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        <location>
            <begin position="521"/>
            <end position="540"/>
        </location>
    </feature>
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        <location>
            <begin position="165"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="549" mass="61710" checksum="8C0304EE4FFB90AA" modified="2017-01-18" version="1" fragment="single">EIDVQNPDPSTVEAVGRHLVNDTDAAKFDSLQLQGGDISREVYNWQREHSQEPRMRRRSQSFSGSISGSLLDPDFQVHDIRVPGGFRRSFLVQKATKQKINGRPQTPTFFTRNFIEFLTLYGHFAGEELRDEDDEDEDEEEEGEEAVDEESLLLEADRQRKHEPHKATTTKAILLLLKAFIGTGVLFLPRGYHNAGWLFSSSALIFFSILSYWCFMSLINVKIKLNINSYGDIGGKLFGPYMRYSILSSIVLSQIGFAAAYIVFTATNLQAFFISIASKHYSIEFFIFVQLLVFIPLSLTRKIAKLSGTALIADLFILLGLVYVYYYCSFVVITEGVGSIKPFNKDWTVFIGTAIFTYEGIGLLIPIQESMQKPSQFPKILGWVMLTVTFVFVSAGGIGYLAFGSDTETVILLNFPQDSLYVSIVQFLYAMAILLSTPLQLFPAIRILENGIFVKSGKYDNKVKWHKNYFRVGVVLSTAFIAWGGASDLDRFVAIVGSFACIPLIYVYPPLLHYKLSANTFWSKAADIAVTALGFSMMIYTSGQTILDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1I6</accession>
    <name>A0A1E3P1I6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_63284</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183521.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3P1I6"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034389">
        <property type="term" value="P:lipid droplet organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019388">
        <property type="entry name" value="FIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23129">
        <property type="entry name" value="ACYL-COENZYME A DIPHOSPHATASE FITM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23129:SF0">
        <property type="entry name" value="ACYL-COENZYME A DIPHOSPHATASE FITM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10261">
        <property type="entry name" value="FIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="290"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="312" mass="36107" checksum="13DE8DF1B6ED1C3A" modified="2017-01-18" version="1">METYQHYYETSLKKFINNKSFLYIYPTIFIVGVVISTIIPSRLFDVLKDNWWLVSSDNFLNKNFAYKGNHIFAIIFTGVFGINLLQELKVQSTNGEKSEGDDAIVKLSWQKFKHFTIKFSMKIVVLFLLFYIIDHIFLMTGGSCVIDKAVANILAKSSKTLEQCTEYNGTWVRLEEGFCLITPAQLSRKISSAQICYKYGEWEGGFDISGHFCFITTLSLILWYELYEFEESYFHNNDVESQKVELVDSSSWLDLTVTNVVRWSVLICLGIWLEILLITAVFYHTFLEKLLGLALGYVGPLFFIISYQELNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1M4</accession>
    <name>A0A1E3P1M4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_13419</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:47005"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038301.1">
        <property type="nucleotide sequence ID" value="XM_019180572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1M4"/>
    <dbReference type="STRING" id="683960.A0A1E3P1M4"/>
    <dbReference type="GeneID" id="30197818"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF565">
        <property type="entry name" value="AT28366P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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            <begin position="23"/>
            <end position="325"/>
        </location>
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        <location>
            <begin position="47"/>
            <end position="84"/>
        </location>
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        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="341" mass="37238" checksum="733C89C99F71AF00" modified="2017-01-18" version="1" fragment="single">MGQILSQPLTDKESESGQNKHLAYGLSCMQGWRISMEDAHTTILDLEKTNNNNNNNNNDDIKTDDDNSNDDNETKTASTSKNTVTDSTDPVAFFAVYDGHGGDRIAKYTGDNLYKLITKEPEFHKGNFGKALQNTFLATDREILEDNELKLDQSGCTATTVLLNSKKIVCANAGDSRTVLSVNGSAKPLSYDHKPNNEGEHARICAAGGFVDIGRVNGNLALSRAIGDFDFKKSTDLPPEEQIVTAFPDIIEHDITENDEFVVLACDGIWDCLSSQQVVEVVRRGINDRKSLVEISESIMDVCLAPSSGGSGIGCDNMSIVIVALLQGKTLEEWYDHIIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1N7</accession>
    <name>A0A1E3P1N7_WICAA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">WICANDRAFT_79771</name>
    </gene>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038460.1">
        <property type="nucleotide sequence ID" value="XM_019184947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1N7"/>
    <dbReference type="GeneID" id="30202193"/>
    <dbReference type="OrthoDB" id="10624104at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="317"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="476"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="499" mass="56502" checksum="FB23609EDEA3CE15" modified="2017-01-18" version="1">MSVDFSITNNAIDFSRLMNDEINSYNQQAQSLLKNDINSTCDYLNEKLDDLIQTFNTTLQMRLNQSIGITTNQINIQSLLNSLNSSIIDSLSQDKANIDNLKATLDKAVYVGDSVDTSNLSLNYDSIQQSLQAAINDQPSVYDNTSIVELNNSSGDIFKQLDEIFQNALDEIQNFNTTIETNQTFYNLQITPNLLSTNITNNNSTTNSSLPPIQSNEFAKQSKINLITIGIFLIISYIFIMIYQYFRFRYQTNYTASRLIDLEKSDSKMDYIGVIQSTNEPTVTMITDIQTRLMTKSKPSEKLFWTNSYLFGDSANLPYLFMMIFILLILMLSLGKIKWGVTNDSGSTDIAYSIIKQYNLTQFNQFKDFGFNDSIIDDYNSNIYDKFQNITSTLQDLFKTHNITAILSLSPPNITHQNITLKPFKSSLETSSLLTNLNNLNFEINTKINTSSIQTPTIISTVLTTISTTFTHIIQIALISTSIAFFLIIFIGILYSFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1W4</accession>
    <name>A0A1E3P1W4_WICAA</name>
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            <fullName evidence="9">PH-response regulator protein palH/RIM21</fullName>
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    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_63970</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
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    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038686.1">
        <property type="nucleotide sequence ID" value="XM_019183647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1W4"/>
    <dbReference type="STRING" id="683960.A0A1E3P1W4"/>
    <dbReference type="GeneID" id="30200893"/>
    <dbReference type="OrthoDB" id="3979655at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071467">
        <property type="term" value="P:cellular response to pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014844">
        <property type="entry name" value="PalH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35779">
        <property type="entry name" value="PH-RESPONSE REGULATOR PROTEIN PALH/RIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35779:SF2">
        <property type="entry name" value="PROTEIN DFG16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08733">
        <property type="entry name" value="PalH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="427"/>
            <end position="462"/>
        </location>
    </feature>
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        <location>
            <begin position="427"/>
            <end position="452"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="462" mass="53431" checksum="BF739148A0481773" modified="2017-01-18" version="1">MATYGGFDVYELTAGTLDVSDGSLFNLSYPAYFLANVTSNVFNSSEFQKDILNISNELYPMFYNKESFMGGVVTISFVLSGIVTASWMLFLLLFLASNTIPNSLILTNLFYSVGHSVMLTKLTSILKCQYKHNYSDMDEIKKLLSFGTSFLIVRSITNLLIWISWVDIMIQISKYSYKRRIIIWGIGLSVCNFVVRLIYGIMYTRYDHHTSKFKGVKALHFFLDYLMLLIFTFQVARYTFQKRKFAYHRKAFALAIFCWITLIVPFIFATIDLCSETLRTWSTYIFSFSVFCVIVVIWEWVHTIRSLELRYERKAVLGRRISNDSFLEDPMNPNLDPKHRHNNGYILKSINVLSILSSFKICGELKNKLTLPSSTARRNTDQHEDVSFFEMVDDLSRQSSYQGSAMSYENDNHPEFNVDHRYLRNTPSTFAGSRVTPISNHLNSEQQTSRTNTPRHDSNDNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1X3</accession>
    <name>A0A1E3P1X3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">sphingolipid C(9)-methyltransferase</fullName>
            <ecNumber evidence="14">2.1.1.317</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_32699</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CFA/CMAS family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.317" evidence="14"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038668.1">
        <property type="nucleotide sequence ID" value="XM_019181997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1X3"/>
    <dbReference type="STRING" id="683960.A0A1E3P1X3"/>
    <dbReference type="GeneID" id="30199243"/>
    <dbReference type="OrthoDB" id="412182at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052290">
        <property type="entry name" value="Sphingo_C9-MT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45197:SF1">
        <property type="entry name" value="SPHINGOLIPID C9-METHYLTRANSFERASE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45197">
        <property type="entry name" value="SYNTHASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G04190)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02353">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="77"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ59461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="501" mass="57953" checksum="74833CBF19126450" modified="2017-01-18" version="1">MEFMGIKYTPTPKADPQPVSSTECGIRTTDYPAIKNAPLPADGPGTKSFSNLALLSFIVLIPFYVKYKLGGGFKTWLFFTLLFVLPILIAYWSFTSTYSPRLNEKVKLPNRGVEYYLEFHDEELAKKYSGQNKVPYETFHELYFAGKVSFKGDALDVLEYRHDWLSFRFTIGLYKYFLFGMIPEVIMHTRSQDEEQVRDHYDRGDDFYAWFLGPRMIYTSGVISDITKEETLEELQDNKLKVVCDKIDLKKGERLLDLGCGWGTLATFASKEYGARATGITLGRNQTKWGNEKLKLQGISQDQSEILCMDYRDTPVPEGGYDKITCLEMAEHVGVRKFGAFLQQVHDMLNDDGIFFLQYAGLRKFWQFEDLEWGLTLMNQYIFPGADASTPLDFVVSKLEGTGFEVVSIDNIGVHYSATLWRWYRNWIGNKDKVVNKYGIRWFRIWEFFLAHSTIISRQGSATCYQIVLRKNINSYHRVEYIPKQKGLLTPIKEGIVDWFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P207</accession>
    <name>A0A1E3P207_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">U3 small nucleolar ribonucleoprotein protein IMP3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">U3 small nucleolar ribonucleoprotein protein imp3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_79801</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038487.1">
        <property type="nucleotide sequence ID" value="XM_019184951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P207"/>
    <dbReference type="STRING" id="683960.A0A1E3P207"/>
    <dbReference type="GeneID" id="30202197"/>
    <dbReference type="OrthoDB" id="10248812at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034457">
        <property type="term" value="C:Mpp10 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140691">
        <property type="term" value="F:RNA folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000006">
        <property type="entry name" value="U3 small nucleolar ribonucleoprotein IMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="12">
        <location>
            <begin position="4"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="RNA-binding S4" evidence="11">
        <location>
            <begin position="109"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="183" mass="21584" checksum="46B7BC289811912B" modified="2017-01-18" version="1">MVRKLKHHEQKLLKKVDFLDWKQDNGHRDAQVMRTYHIQNREDYHKYNKICGEIRKLAKKLSELQPNDPVRIKHEQLLLEKLYSMGILSTKSKISDLENKVSVSAMCRRRIGVVMCRMKMAQSIKDAVTFVEQGHVRVGPNVITDPAFLVTRQMEDYLTWVDDSKIKENVLKYRNKIDDFDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P235</accession>
    <name>A0A1E3P235_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_93673</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038169.1">
        <property type="nucleotide sequence ID" value="XM_019186426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P235"/>
    <dbReference type="GeneID" id="30203672"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="51" mass="5822" checksum="570F91A9DDEDEFE6" modified="2017-01-18" version="1">MSMMLGTLMDSYNSKAHNSMFQGHHVNMIFLTQNILLEILNKSNIVLSSHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2C4</accession>
    <name>A0A1E3P2C4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Diphthamide biosynthesis protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_63850</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the first step of diphthamide biosynthesis, the transfer of 3-amino-3-carboxypropyl from S-adenosyl-L-methionine to a histidine residue. Diphthamide is a post-translational modification of histidine which occurs in elongation factor 2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DPH4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038561.1">
        <property type="nucleotide sequence ID" value="XM_019183624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2C4"/>
    <dbReference type="STRING" id="683960.A0A1E3P2C4"/>
    <dbReference type="GeneID" id="30200870"/>
    <dbReference type="OrthoDB" id="445556at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.660.10">
        <property type="entry name" value="DPH Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044248">
        <property type="entry name" value="DPH3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007872">
        <property type="entry name" value="DPH_MB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036671">
        <property type="entry name" value="DPH_MB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454:SF46">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454">
        <property type="entry name" value="DPH3 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05207">
        <property type="entry name" value="Zn_ribbon_CSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144217">
        <property type="entry name" value="CSL zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51074">
        <property type="entry name" value="DPH_MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="J" evidence="11">
        <location>
            <begin position="8"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="DPH-type MB" evidence="12">
        <location>
            <begin position="88"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="163" mass="18289" checksum="C130A3C631847FD6" modified="2017-01-18" version="1">MANISQKTHYEILGVERGSNVQEIKNAYKEKLLNTHPDKTNKQLIDSDIIAQIKLAYATLVNDTLRTQYDEELKQTFQRNGLISTGEGLDIVTLDDFSYSEDDGFSKDCPRCTATGSFILTEDDLAESGTADGLGGYEIIVQCNACSLWLKVKYFDLEDDEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2F0</accession>
    <name>A0A1E3P2F0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bacterial surface antigen (D15) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_105904</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAM50/omp85 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038284.1">
        <property type="nucleotide sequence ID" value="XM_019180456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2F0"/>
    <dbReference type="STRING" id="683960.A0A1E3P2F0"/>
    <dbReference type="GeneID" id="30197702"/>
    <dbReference type="OrthoDB" id="1724197at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.50">
        <property type="entry name" value="membrane protein fhac: a member of the omp85/tpsb transporter family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815:SF18">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY COMPONENT 50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY SAMM50 PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="Bacterial surface antigen (D15)" evidence="6">
        <location>
            <begin position="172"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="492" mass="55644" checksum="CC1CF611684731DC" modified="2017-01-18" version="1">MSLDLKDDDNVINRITNGFKPKDQELSQLDKLEIEKHQIIQKQGKLFLEELFRNNSTRPIHVSKVQIEGTEQFRDSFLTAQILPFINDSQNLTKLLTNVDKVSSNFFKTSTIDNFAINLTTPPYRSSSHIPENAIEIIPHIKLFPVKKFMAKTGTNIGNGEGDGYMTFQFKNIFGGGENVIFDATTGTRTRSSYLLNYSSPFFNNANWKSDNSLFITSRKIDWSSHEQLIKGLNNKLVSINLGNESQINHEFSVENILRSITNVSPLASNNVLYHAGDDFKTSLIYKFGFDSRNDRTLPTRGAYFSLINEISGIFPKFNTSKFLKQSFESAYATNFNDGDQILNISFRGGWLYSFKNTTHLMDRFYLGGPNDVRGFFFNGLGPRNFNDTLGGDVFLSGGISLFNRIPFVSKESNFKLHSFVNFGRLIPLDKNEHISHTVKELLSEPSIGAGFGLVFKHPVARFELNFVLPVTAHENDSSRKGLQYGIGLTFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2G5</accession>
    <name>A0A1E3P2G5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_63892</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038601.1">
        <property type="nucleotide sequence ID" value="XM_019183630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2G5"/>
    <dbReference type="GeneID" id="30200876"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="77" mass="8777" checksum="2B42B90FA1837EA5" modified="2017-01-18" version="1">MTESNVINSPPEYLKNQPPSYNQEMVSRSEQYNLQRTTTEQKLGIPDNDPDEDDGCIYRWCVILHCNTVLTNAATGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2L3</accession>
    <name>A0A1E3P2L3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Indoleamine 2,3-dioxygenase</fullName>
            <ecNumber evidence="5">1.13.11.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_91366</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Produces N-formyl-kynurenine through the oxidation of tryptophan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-tryptophan + O2 = N-formyl-L-kynurenine</text>
            <dbReference type="Rhea" id="RHEA:24536"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58629"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the indoleamine 2,3-dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.52" evidence="5"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038842.1">
        <property type="nucleotide sequence ID" value="XM_019186291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2L3"/>
    <dbReference type="STRING" id="683960.A0A1E3P2L3"/>
    <dbReference type="GeneID" id="30203537"/>
    <dbReference type="OrthoDB" id="540174at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033754">
        <property type="term" value="F:indoleamine 2,3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.480:FF:000004">
        <property type="entry name" value="Indoleamine 2,3-dioxygenase subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000898">
        <property type="entry name" value="Indolamine_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037217">
        <property type="entry name" value="Trp/Indoleamine_2_3_dOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28657">
        <property type="entry name" value="INDOLEAMINE 2,3-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28657:SF5">
        <property type="entry name" value="INDOLEAMINE 2,3-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01231">
        <property type="entry name" value="IDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140959">
        <property type="entry name" value="Indolic compounds 2,3-dioxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00876">
        <property type="entry name" value="IDO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="4 5" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="proximal binding residue" evidence="4">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600898-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="475" mass="53529" checksum="71664897F6F82788" modified="2017-01-18" version="1">MIGLPNLKDYDVSPKTGFLPEELPLTRLTSEYYKPWESLVEVLPSLILTKRIRKLVEKLPVLSIDQIKGDKKELRRAYSVLSFLTHAYVWNVDVPTDTLPKPLAEPLLEVSKILELPPVATYSGLILWNFKPILDMGEDEEFDLDNLTTINTYTGSIDESWFYLVSVYFEYKGAHCITDGLDAIKAIRENNKEKVIDSLQKLAESIDRLGSVLMRMEEMCDPHTFYYRIRPYLAGWKNMKDAGLPNGLKYGDEGHYRSYAGGSNAQSSLIQTLDIMLGIEHYPTGEKKDSSSSKSISSLSGQNNFMNEMKTYMPGPHQRFLNHLAQVANIRDYVAEVQDPELTLSYDACLAMLKAFRDKHIQIVTRYIILQAKLYKKAGSSNTLRSGISKTAEKTEQKGTGGTSLIPFLKQCRDETGDLAAGKWGKKILSAGVLNSRYTSKMEIEDDETEHVNKKAKLGLAGSWVDGGEEQTGHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2R7</accession>
    <name>A0A1E3P2R7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glycosyltransferase family 20 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_45032</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038691.1">
        <property type="nucleotide sequence ID" value="XM_019182586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2R7"/>
    <dbReference type="STRING" id="683960.A0A1E3P2R7"/>
    <dbReference type="GeneID" id="30199832"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005946">
        <property type="term" value="C:alpha,alpha-trehalose-phosphate synthase complex (UDP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000099">
        <property type="entry name" value="Alpha,alpha-trehalose phosphate synthase subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000036">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase subunit Tps2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1020">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase related protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF15">
        <property type="entry name" value="TREHALOSE SYNTHASE COMPLEX REGULATORY SUBUNIT TPS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="861" mass="98168" checksum="3E195AC703FD93DB" modified="2017-01-18" version="1">MFLNQVGSTSSTFIPKSRVNSPPPNSVVEPKKSNRFELGVPAVKRIHQKSNLKYQTNEQDVDNEKTTENISNIHRNSINNLELDDDYDNGRTYELPKFGGYSSNDKKIFNHHDKSNIFETAPFNVVEFAKGNGGLKNAINSSLEDGIVKDYVWLGTIGIPTDELPNNTKTKITNKLRNDYHSESVLPSDFTFQGHYKSFCKQILWPTFHYQIPDNPNSKAFEEHSWEHYRNLNQEFADKIVEIYKDGDIIWVHDYHLLLVPEMVRSKLPNAKIGFFLHVSFPSSEVFRCFAQRENLLKGLLGANSVSFQTDEYARHFLQTCNKLLLADITDNELRYKGQTVKVASSPVGIDANKLIKQQHSELVKNWRHLIRERWPNKQLVVARDKFDRIRGVKQKLLAYEGFLKKNPKFIESAVLIQVCLRSSATDPVLETEIMSIVDRINSLSPNISNTQPVVFLHQDIDFAQYLALISEADLFVVSSMREGMNLTCHEFIVATNEKKSPLILSEFTGSADVFTKGAILINPWDIKQVSESYEIAFNLTQTEKEQNWKNLYNVVMKQDCDYWVESSLKFIEDSWRSQQEKKNYHNLDVEKFISSYKDSQNRLIILNLAVIPTERILQILTELSFNNDVYMLSTFKRIDLERLYRRVPRLGLIAENGGFIKLKNGGPWLSISNKNNDDWIETTSKVANSFAERLPGSYVEIGECTLKFHTEQVEDQERKSSSIGDLIFHVNNSDSNIHATLINNTVIVQESDLTLKAVKLLINYITKTGKINDANLITPASPTSPISGNEQMEFLPKKLDFLTIAGNSSLLIIEPVLEYANELKNVDHVFTIAFGGDVSNAKEHVEGLNELFTVLNSAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2V5</accession>
    <name>A0A1E3P2V5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_78285</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038855.1">
        <property type="nucleotide sequence ID" value="XM_019184696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2V5"/>
    <dbReference type="STRING" id="683960.A0A1E3P2V5"/>
    <dbReference type="GeneID" id="30201942"/>
    <dbReference type="OrthoDB" id="205794at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008409">
        <property type="entry name" value="SPF27"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05700">
        <property type="entry name" value="BCAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="124" mass="14219" checksum="53FA6525602F8C25" modified="2017-01-18" version="1">MNYEPLDSLPYIDEDVTDEERQNIEKLILEELSLIKLNEIHPKVKELYTLPQPHHLIADIGEEELTDPGFSLGGIDLSDYSNLENTESLQKSLVFTSLRNKSLKLSSQFVMTNLNIQMNKSNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2W2</accession>
    <name>A0A1E3P2W2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_78460</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039033.1">
        <property type="nucleotide sequence ID" value="XM_019184723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2W2"/>
    <dbReference type="STRING" id="683960.A0A1E3P2W2"/>
    <dbReference type="GeneID" id="30201969"/>
    <dbReference type="OrthoDB" id="1726119at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051727">
        <property type="entry name" value="DnaJ_C3_Co-chaperones"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44140">
        <property type="entry name" value="LD25575P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44140:SF2">
        <property type="entry name" value="LD25575P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0802">TPR repeat</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133637" description="J domain-containing protein" evidence="6">
        <location>
            <begin position="18"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="70"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="463"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="423"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="526"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="441"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="460"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="548"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="570"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="597" mass="68900" checksum="163B7A597DCC8502" modified="2017-01-18" version="1">MKPSIIIISLLSGLVVASSNDLNSKLLVIDSDFQKSGPSLDILNDYDVLLRQLNTEKDITSELERSNILSKIYYKKAIVELSLGKDESSIEDFSKSLEYNSNNPVVRKKLLDLCIKYGLKDRIYSLKKVFNGEDPDYLDALNTLNVVDALVASDDLGKLNEAINISPFDSSLRLKRIELTTQDIKQTGDSLKFLDLVDDMTSLIKTNPINHLEFINSLSEIYIFALSEFSNALNFNKKCLHYDMDNTLCKKNSKFLNKYSPILLNLSNFNKFFQFLEDKDKSDQDVDLDDEDMKMEATRLQDPSKFLKIRRENENFKTNQEYLLRKSQEFNTQYNLKTNSLQLTILKTLIFDAFIRNDHKSFKSLSKKLNSIQENASETFLPLILRNVDMAIDKNDYNQAIQLLKQVTENGKKTVYYDTRAQKIQHHQNQQRQHQQREQQQRQQQYYQQQQQHQQRSRTPKNDYYKILEVPKDADQTTIRKAYREKTKQYHPDKYKGELSQQDIETKMTQINHAYEVLSDAELRKNYDQGQDPNDPESMHGGGGNGGMRQNPFGGHQNPFGNGGFNFQFGGPGGFQFGGGQGGGGFKFRDGKRKRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2X8</accession>
    <name>A0A1E3P2X8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_91776</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039046.1">
        <property type="nucleotide sequence ID" value="XM_019186321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2X8"/>
    <dbReference type="GeneID" id="30203567"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="80" mass="9097" checksum="E156FC062F5B7C61" modified="2017-01-18" version="1" fragment="single">MSEEVKETPVAPAPVEESKEAKEAEVEAKPTESKEEVKESKEEETKEEKPAETKEEAKEDSESKRKPEEQPAKKEKRDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P337</accession>
    <name>A0A1E3P337_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_83918</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039103.1">
        <property type="nucleotide sequence ID" value="XM_019185618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P337"/>
    <dbReference type="STRING" id="683960.A0A1E3P337"/>
    <dbReference type="GeneID" id="30202864"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17327">
        <property type="entry name" value="MFS_FEN2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000064">
        <property type="entry name" value="MFS allantoate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF1">
        <property type="entry name" value="ALLANTOATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="434"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="49"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="496" mass="55454" checksum="02D03DCF217A9C48" modified="2017-01-18" version="1">MSTEPSNNDHINHHKTSVDAAFDLSLQSQGIEITPEINKSLLRKIDLFIQPIIWAVYTLQYMDKVTNNYAGVMGIQRDLKLHSNEFSWLGTGFYLAYLIAEFPISQALQRLPFIKTLSSCIVIWGIVLCAHAGAHNSAQIITARVFLGVFEASVTPGFVILSSQWYRKEEQFARIAYWFSCNGAGNLIGGAIAYGLAHHAKSLSIEPWRVLFIVTGLMTILFGVIFFFYVPENPSKAWFLTDEEKLYQVQRIRKNQQGYGTRKFKKYQVIEAFKDVRTWIYIIWGLTAQIPNGGLGSFGSILLNQTLGYSKEKSLLMGLPQGAIQVASGIITGYIATRTGKRVIVGVGCCIITLIGSCLLAFTHHPHSQLAGYYLFPFYAIGMVTILASITSDAAGHTKKLTVSATFLVSYCVGNVIGPQTFRDKDAPQYQPARVTMAACLAVCTVDLFILLVVNTFVNNQRDKKFAEDPNYYTAPENSEFLDLTDFENKNFRYSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P372</accession>
    <name>A0A1E3P372_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Glycosyltransferase family 71 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_17946</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038544.1">
        <property type="nucleotide sequence ID" value="XM_019180769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P372"/>
    <dbReference type="STRING" id="683960.A0A1E3P372"/>
    <dbReference type="GeneID" id="30198015"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF1">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="470" mass="54656" checksum="3D2DFEA2ABB7B7F2" modified="2017-01-18" version="1" fragment="single">QSHIKDQRYNKKSQKFYIKMFKMLNEQKFKIPALDLIGKADSRDPNLIWSKSKLSEFLKLDPLQVENLHKIHEDVFNQLPYKFPKNLYRGNGIVLIGGVQYSWLSLLSIKSVRSFGSNLPIEMIIPTPEEYEQELCEIVLPSLNAKCVLLHNVFGEEVMDTVKFSGYQYKSLALIASSFENVLLLDSDNILVSNPDELFVKEPFKTSGMVLWPDYWTRTTSPLFYDIAGINVTNTRVRKGKWPLIPSEIVEELDKVPLHDLEGSIPDLSSESGQLLIRKTDHLKTILLSLFYNLYGPNFFYKLLSQGQSGEGDKDTFIAAAIKSNEPFYQVKSEIQSFGWFDEGNFNGVSMGQRNPIQDYEYYQNFLKTLEPKFEEYDTRAAPIFTVHANFPKLNPYLLWKEKKLFNSKNEEVRLYSDISNFLPEPFDFELVQYRRMKSLLCDLKIKLNYFEDVDKESVCKFIDNHITFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P381</accession>
    <name>A0A1E3P381_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Striatin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_62459</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039082.1">
        <property type="nucleotide sequence ID" value="XM_019183374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P381"/>
    <dbReference type="STRING" id="683960.A0A1E3P381"/>
    <dbReference type="GeneID" id="30200620"/>
    <dbReference type="OrthoDB" id="727118at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013258">
        <property type="entry name" value="Striatin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051488">
        <property type="entry name" value="WD_repeat_striatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653:SF0">
        <property type="entry name" value="CONNECTOR OF KINASE TO AP-1, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653">
        <property type="entry name" value="STRIATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08232">
        <property type="entry name" value="Striatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Striatin N-terminal" evidence="4">
        <location>
            <begin position="15"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="137"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="34"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="174"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="201"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ59875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="540" mass="61140" checksum="D75AA41A2C6392CE" modified="2017-01-18" version="1">MSAAHPQNVSHLEYSLPGIMHYLQKETTKNERDRVQWELEKGELKSNITKLEGENKSLHEEVSRLKAELGHVNNSKNTKVNKAEVSKIKFKEVDVNPLAHAREKLQSQIKEIGVLLNATPVDLSKLQLDSDRAYYSTYPGLSQQDESTAESSLNSNQVAQPKEAVKITNSSPIQKKETITTFEKPKPDPAPEFKPETHDVDESDAETVTGETEEEKEPSQQQQPQNNHKGIEKFLLPHFATITSFASNGKELLAASEGGVIKYWSIQDILNSKGALESLKSYRADSELIEFVKWIDKDHFVTVSGNTVRFFDKNSTQPPSVKYNINSVKSVDANNSHIAHVANDGSLTISNLTYSPHIKYEKVKDRTEKCDFVAFDTKNEHELITVKQDGVVEVINLETKKKMTPILSINLTGKTITNFTVEEKLWCFELNKNEVILYNSTSKSVTFQQKYEVEIAKVQISPKLIVIFLTDGDIKVYETKNSKSEIFKDYNIYKLFFTDHSNLTKEDKVTLKNSLKTGILVFNDYVFGGCEDSFIRGFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3D9</accession>
    <name>A0A1E3P3D9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BOD1/SHG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_78462</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039035.1">
        <property type="nucleotide sequence ID" value="XM_019184724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3D9"/>
    <dbReference type="STRING" id="683960.A0A1E3P3D9"/>
    <dbReference type="GeneID" id="30201970"/>
    <dbReference type="OrthoDB" id="5579731at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055264">
        <property type="entry name" value="BOD1/SHG1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05205">
        <property type="entry name" value="COMPASS-Shg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BOD1/SHG1" evidence="1">
        <location>
            <begin position="9"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="139" mass="15435" checksum="E29A87AF633A4F0D" modified="2017-01-18" version="1">MSSNDAKSLSISYKRKGYFDDKRKSILKEFKTTNAHDQLLSTIKEIVNKIVHKNPELLLKNKGQLAALIEGTLSRQIQNTGGLNNTTTANVPGNNTSSIDLGDVGNVYDIFDEEVKQSTIGSDELKEEIRAVLKELKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3H3</accession>
    <name>A0A1E3P3H3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_62340</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038961.1">
        <property type="nucleotide sequence ID" value="XM_019183345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3H3"/>
    <dbReference type="GeneID" id="30200591"/>
    <dbReference type="OrthoDB" id="10491714at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="194" mass="21871" checksum="4415A0547E6AF6B5" modified="2017-01-18" version="1">MSLPPANSDPRVYQIRLTTQNLYSLLFNSFQTISNLASTYNQIATASTNKVLKDDIAWLKESIDKDIEKLNALQKHLQFLNAQETITTPGELLKVFNEITDFAQLILLDDLITTLEGIGSVITEDELMIDGVGLKDVVILLKKFSISLKLAVDPLKKLKDEEVSVIQLEKSEVIITERVEDLKKRVTELENIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3J8</accession>
    <name>A0A1E3P3J8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_85010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59457.1"/>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019185824.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="30203070"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="360" mass="41080" checksum="61E1E6A929BDCE04" modified="2017-01-18" version="1">MAAPLSSIIKNENNLKRTKSFGIQNQCGVSFAPNKKICLSPMLSRRNSATIAALYNDNSSSESSDEDEDNSQDESCYMNNQRRARKRAFSLKRSLSFADNKLNDAVSKYSDVDEDEEEEESKKHHSSRRSIPSTAGNSDVEKKSLKKEIESVVPELKKSHHRDSITSQSFSGDSKSQIRRSSIDCDQVARSRCFDYLVTAIDEAWARYCDATTYAEDEVYNNVDHLPNTPASLNFSDDNEDEDEEGDGDNNDDVYDEVDDGYKSASTNLTEYDSDFELKRRVSSQPSSVRLQQLKDRLLKAKYYLQDFIESDSFDEACAFWKRWDLIKYATIELVEDDDDDEVIESTIEDLEKGRFYGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3R6</accession>
    <name>A0A1E3P3R6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar protein sorting-associated protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_104632</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038749.1">
        <property type="nucleotide sequence ID" value="XM_019180421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3R6"/>
    <dbReference type="STRING" id="683960.A0A1E3P3R6"/>
    <dbReference type="GeneID" id="30197667"/>
    <dbReference type="OrthoDB" id="29072at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990621">
        <property type="term" value="C:ESCRT IV complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070676">
        <property type="term" value="P:intralumenal vesicle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090148">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036258">
        <property type="term" value="P:multivesicular body assembly"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:protein retention in Golgi apparatus"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02678">
        <property type="entry name" value="MIT_VPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19521">
        <property type="entry name" value="RecA-like_VPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000043">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000015">
        <property type="entry name" value="vacuolar protein sorting-associated protein 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000014">
        <property type="entry name" value="Vacuolar protein-sorting-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045253">
        <property type="entry name" value="VPS4_MIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF83">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04212">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIT" evidence="11">
        <location>
            <begin position="3"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="162"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="71"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ59542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="429" mass="47613" checksum="2A856F04DD40C894" modified="2017-01-18" version="1">MSTGDFLNKGIELVQKAIEADNATRYDEAYQLYYNGLDYLMLAIKYEKNARSKELIRTKFTEYLSRAEQLKEHLDKKEKQAEASANGSTKSKKSGNGNGSGDDEDDSDNKKLRGALSSAILSEKPNVKWEDIAGLEPAKEALKEAVILPVRFPHLFTGKRKPLSGILLYGPPGTGKSYLAKAVATEANSTFFSVSSSDLVSKWMGESERLVKQLFNMARENKPSIIFIDEVDALCGPRGEGESEASRRIKTELLVQMNGVGNDSSGVLVLGATNIPWQLDAAIRRRFEKRIYIALPEVEARARMFELNVGSTPCQLGSKDYKLLGEMTDGYSGADISVVVKDALMQPIRKIQSATHFKRTEENKLTPCSPGDSDAVEMNWMEIEGDELQEPELTIKDFIKAIKTTRPTVNEADLKKQIDFTNDFGQEGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3X9</accession>
    <name>A0A1E3P3X9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">WICANDRAFT_53842</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039332.1">
        <property type="nucleotide sequence ID" value="XM_019182723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3X9"/>
    <dbReference type="STRING" id="683960.A0A1E3P3X9"/>
    <dbReference type="GeneID" id="30199969"/>
    <dbReference type="OrthoDB" id="10248617at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005721">
        <property type="term" value="C:pericentric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140727">
        <property type="term" value="P:siRNA-mediated pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.150:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1070.20:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1110.10:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1800.10:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007175">
        <property type="entry name" value="PRK09606.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="19">
        <location>
            <begin position="34"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="18">
        <location>
            <begin position="218"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="20">
        <location>
            <begin position="480"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="21">
        <location>
            <begin position="579"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="22">
        <location>
            <begin position="678"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="16">
        <location>
            <begin position="752"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="17">
        <location>
            <begin position="1125"/>
            <end position="1217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="ODQ60125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1223" mass="139412" checksum="92F2A0FC249172AA" modified="2017-01-18" version="1">MSEEYDVDPYAVEADDITSEDCWTVISAFFREKGLVSQQLDSFDEFIHTSIQELVWEDSTLILEQPAQHITETDNINRKYEIKFGKIFLSRPTMTESDGTTTQMFPQEARLRNLTYAAPLFVEMDKITYECIDGENDWKVIKDEVDMDDDDEFGSSSKIDKKVFIGRVPIMLRSKYCLLRDLGEEEFYSLKECPFDMGGYFVINGSEKVLIAQERSAANIVQVFKKSAPSPVSHIAEIRSALEKGSRLISSMQIKLYGRDDKGSGRTIKATLPYIKQDIPIVIVFRALGIVPDGDILEHICYDQNDWQMFEMLKPCVEEGFVIQDREVALDFIGRRGNALGIRREKRIQYAKDILQKEFLPHITQQEGFESRKAFFLGYMVNRLLLCALERKPQDDRDHFGKKRLDLAGPLLANLFRILFKKLTRDIYRYMQRSVETDTDFNLKLAVKATTITSGLKYSLATGNWGEQKKAMSSRAGVSQVLNRYTYSSTLSHLRRTNTPIGRDGKLAKPRQLHNTHWGLVCPAETPEGQACGLVKNLSLMSCISVGSGSDPIIYFLEEWGMEPLEDYLPHQAPDVTRIFVNGVWHGIHRDPAKLIATMRNLRRRGEISPEVSIIRDIREKEFKVFTDAGRVYRPLFIVEENEETGAKELKLKRDHIERLYDAEYNDYQDTDEEPFTWNSLLTNGIVEYVDAEEEESIMIAMSPEDLNPDTYAGLEDEDVNSNKRLKPSFKKSAQTFTHCEIHPSMILGVAASIIPFPDHNQSPRNTYQSAMGKQAMGVFLTNYSVRMDTMANILYYPQKPLATTRSMEYLKFRELPAGQNAIVAIACYSGYNQEDSMIMNQSSIDRGLFRSLFFRSYMDQEKRVGMTVVEQFEKPNRSNTLRLKHGTYEKLDDDGLIAPGVRVSGEDIIIGKTTPIQADSEELGQRTVYHSKRDASTPLRSTENGIVDQVLITTNQEGLKFVKVRMRTTKVPQIGDKFASRHGQKGTIGITYRREDMPFTREGVVPDLIINPHAIPSRMTVAHLIECLLSKVSALSGLEGDASPFTDVTVDAISKLLREHGYQSRGFEVMYNGHTGKKLMAQIFFGPTYYQRLRHMVDDKIHARARGPVQILTRQPVEGRSRDGGLRFGEMERDCMIAHGAASFMKERLMEASDAFRVHICGVCGLMSVIAKLKHNQFECRGCKNKIDIYQIHIPYAAKLLFQELMAMNIAPRLYTDRTVPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3Z1</accession>
    <name>A0A1E3P3Z1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zinc-ribbon 15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_30791</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039423.1">
        <property type="nucleotide sequence ID" value="XM_019181762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3Z1"/>
    <dbReference type="STRING" id="683960.A0A1E3P3Z1"/>
    <dbReference type="GeneID" id="30199008"/>
    <dbReference type="OrthoDB" id="5545479at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28139">
        <property type="entry name" value="UPF0768 PROTEIN YBL029C-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28139:SF1">
        <property type="entry name" value="UPF0768 PROTEIN YBL029C-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="9919" checksum="BF9DB6C3BC552FA8" modified="2017-01-18" version="1">MSFVPIIFGKKDMDSRIDGYPNNLPCPNCHNQSVEPVKRKEFISFWWIPLIPMYWGKQLKCNTCSWRQDVNKEELEKYGIKHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P403</accession>
    <name>A0A1E3P403_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_68627</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039290.1">
        <property type="nucleotide sequence ID" value="XM_019184129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P403"/>
    <dbReference type="STRING" id="683960.A0A1E3P403"/>
    <dbReference type="GeneID" id="30201375"/>
    <dbReference type="OrthoDB" id="190098at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051601">
        <property type="term" value="P:exocyst localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035544">
        <property type="term" value="P:negative regulation of SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.50:FF:000006">
        <property type="entry name" value="Exocyst complex component sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.70">
        <property type="entry name" value="Exocyst complex component Sec6, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010326">
        <property type="entry name" value="EXOC3/Sec6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042532">
        <property type="entry name" value="EXOC3/Sec6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06046">
        <property type="entry name" value="Sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="708" mass="82846" checksum="46291874217AC547" modified="2017-01-18" version="1">MIYVYCLLRMDELEINDYVDLYELINKITKIHEIFDQSTDIVENISSFNNNLQEVEELIETEDYENVDIDFEIPSLLEIHYKLNKLRDFQERLLLLSDSSNEDHKFIVKRVVSRIYDVIKKFDKLLENIIIGLVESVKINNNSLVIRLAKIIDFEEREDTKIDALKGIISKNSKNNSQNSLFEQIVNGTIKGRTTARNYKQFFIDKVEESIHEIFQNCYETYKQKENMFEILDNLDWVFHDLICVKQDLVKLVPPRWNIFTVYYDFYYRELNILINKLIESEPETLIILEILDFDNKFKDIMISEFGFTKQENKSIIGDVEKEKLLSDYLNLIVLKMNEWMFNLQKTEHEVFRSRIQAPEVDSENLYALEGTKIVFQMFTQQCDVASGSGQGKILAGVVEEFGGLLIKRQSKWGELIKNEVQKLLTLNNKSKKDVGVDGVNDDDKVPPGLIEYLIALANDQMRGADYTEAISNKYGGMVSSKYSTIIHNALEKVIDGFANLAKQCCDALIVIIFDDLKIALDEIFSKDWYNSNYSQQVSDTLYEYLGDLKQSMNSYLFEILSEEIVEETVLRYLSNLNKDVKFKNNNDKFVNSVKRDFEIFYKLFINFIDQEIIESKFRIVEDFMDLSTETNDDHIVELWIQLVSKFNDISIDFLALILKARKDIDSTPSKAILSKSKIEQDKFLQENQYELTPSFMNRFVVTKKLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P458</accession>
    <name>A0A1E3P458_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nuclear distribution protein PAC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Lissencephaly-1 homolog</fullName>
            <shortName evidence="11">LIS-1</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">nudF homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PAC1</name>
        <name evidence="11" type="synonym">LIS1</name>
        <name evidence="13" type="ORF">WICANDRAFT_29770</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Positively regulates the activity of the minus-end directed microtubule motor protein dynein. Plays a central role in positioning the mitotic spindle at the bud neck during cell division. Targets cytoplasmic dynein to microtubule plus ends, thereby promoting dynein-mediated microtubule sliding along the bud cortex and consequently the movement of the mitotic spindle to the bud neck.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Self-associates. Interacts with NDL1 and dynein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">Cytoskeleton</location>
            <location evidence="11">Spindle pole</location>
        </subcellularLocation>
        <text evidence="11">Localizes to the plus ends of microtubules and the mitotic spindle poles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the WD repeat LIS1/nudF family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038889.1">
        <property type="nucleotide sequence ID" value="XM_019181649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P458"/>
    <dbReference type="STRING" id="683960.A0A1E3P458"/>
    <dbReference type="GeneID" id="30198895"/>
    <dbReference type="OrthoDB" id="10264588at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051012">
        <property type="term" value="P:microtubule sliding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03141">
        <property type="entry name" value="lis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017252">
        <property type="entry name" value="Dynein_regulator_LIS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037190">
        <property type="entry name" value="LIS1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037647">
        <property type="entry name" value="Dynein_regulator_Lis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109925">
        <property type="entry name" value="Lissencephaly-1 protein (Lis-1, PAF-AH alpha) N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 11" id="KW-0813">Transport</keyword>
    <keyword evidence="3 12" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="104"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="164"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="196"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="362"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="443"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="457" mass="51332" checksum="C82565167F90D599" modified="2017-01-18" version="1">MSLLSAKQRSDLNTSILNYLKPLVSQDCITNLQQELQIDELKDDGDLLTKKWNAIIRLQKKIIDLESQVDQLSKQSTNSSYLTSSNTNSDLVKLNWVPSKVKKTLNNESSVTCAAIHPSLPQLVNGCSDGSFTVWNVLDLAQPIDIIQAHTKTLNKLVISPDKLEFNNNNHVMVTCGSDLYVKVWDCSNYKLLRTLTGHEHVVSSVVFKEGSRYIFTCSRDTTIKIWDLTNGWCLKSFIGHSDWVRTLDLTASEYLLSGSNDQSIRLSHGDSGTGLGIMIGHNQVVEVVKFLPTLSNQYVDKMLTNLESVDQDSYDKLGYKYAVSGGRDDTIKIWLLPIPIIRPHNHPIPSSNPQGTLIKTLIGHKSWVKDLIIHPNGKFLISCSDDKSIKIWDLENGDCLRTLQNHHMGFVNSIAFAPPIFENPNDRKTHDQALINESMRCLFISGGTDQLVKVWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4L8</accession>
    <name>A0A1E3P4L8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_92517</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039465.1">
        <property type="nucleotide sequence ID" value="XM_019186367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4L8"/>
    <dbReference type="STRING" id="683960.A0A1E3P4L8"/>
    <dbReference type="GeneID" id="30203613"/>
    <dbReference type="OrthoDB" id="2285229at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF59">
        <property type="entry name" value="EXORIBONUCLEASE II, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNB" evidence="2">
        <location>
            <begin position="552"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="980"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="980" mass="111220" checksum="CD98BD9E074CF260" modified="2017-01-18" version="1" fragment="single">MLQAVRSTSRFNKQNARRFSSAAIALRSKKSRPSASSKSFLNEKDIDQMIEEDHDSAEDKRELSEDDKTKLYDSVSNLLNSDTFKRIISTETNSKKSLNKISFDEKKRFSEKFREPTAKWITKIKQDLGKELEKDVSEIPIAALLKEAYGSPKRYLKPLDIFESLKLGDAVEMSTALNQNVMAVVIEVPSRDDDPRYTIMNRKGELLFVEKSAFKLRIPGLIPRGWVEGIVEETKYESPLYGSIKTTVDHKFESYFVNPLARRVIMDPILRITNEAWDKLPDISRRLEILHRVVQDRGPKQISLFALAEAVNLIDPENFKNNLQNNDATSQSEYLKLSEKLHESVGLEYNLHKQSDLGREISSSNYGDKVSISHLYAVILALRKQARLWSTSYSSRSAMMPTSVTVNSLEYVNKIDGVIESLKKDTQLVKEFMDFVEKEDYSVIPPQFEGILNLLKEYSVDNITDPVAETVICQLMKNMKNIEKSNITKTHVYDLLINIGYISNSLTNPQHFSNNLCIPGKGVSIKADHEQDYFDLVEISEDGVPDVSESIRKDFGDMRVYCIDSETAHEIDDGVSIEKIDKENFVLHIHIADPSSYLKPGDTVSKIAFERAFTTYQPELVSPMLPKTLSDLCGLGVDGRKTRAITFSVPFSYKYGANLKEAKVQPSYVSNFPKYTYDMVDETLKKTKDQTYETMNNEQRELNDLFNVAKRLQKRRIDNGAIAFGDTIQTNVSVSKNSIKPETALIDSSLDEGSDVLFTKQTLSDSVILVSELMILANSVGAAVMRKFKIPGIFKGMRSLELSGSAVNAVSRLNTYSKETDKLPGLADVVKSIKFVNPAFYFPYPIRHLMLGINGYLPSTSPLRRYGDLVNHWQFHSYLNTGKPLFDSQQMVNMALHIETRNDILKRAQRLSNTFYTLSHIKHELKTGNQLKLSFMVWSRPHDNYVAGMITEFGVFARLALNLNKQPPKIGDVITDFEIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4N0</accession>
    <name>A0A1E3P4N0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNA polymerase II elongation factor ELL N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_78850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039447.1">
        <property type="nucleotide sequence ID" value="XM_019184797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4N0"/>
    <dbReference type="STRING" id="683960.A0A1E3P4N0"/>
    <dbReference type="GeneID" id="30202043"/>
    <dbReference type="OrthoDB" id="2587563at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="392" mass="45180" checksum="2B50065ADC63316F" modified="2017-01-18" version="1">MPTQKLLPLVSGSSNQPNNQNSSIPKAFNIKIPLEIQQKLINSNSNLKIIVSKGELQLNINGQIYKLNKLQEYSKVDLYKQFSNSTFKSIGTIHSKLTISQTSTPPVPPPTKTHTPPIQSHSRNRSNGSNNTNPNNNTLIASGTKISAITKKLVHLLALGPISIEDMVQRTKVSKNDVESVLRSISMVYSPQKQKQIINKYPYEPEKKEEEGEVLYVLNYSNYKELRLNDWKYSMEELKQIKENCKAVYNHLNYPSSHPARLVLDPKEKELKSETQRPTEPKREPKVEPKGEPKGEPKSESNGTIPEHKIDQPIRKEPVSLKRKRSDEEDIESLQNLAKKFKSKYKEYETLYKEIIKNPNHEIKLTKLYQMHKDLEVWKKQLWNYNDNNNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P578</accession>
    <name>A0A1E3P578_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">HRDC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_29640</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the exosome component 10/RRP6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039204.1">
        <property type="nucleotide sequence ID" value="XM_019181633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P578"/>
    <dbReference type="STRING" id="683960.A0A1E3P578"/>
    <dbReference type="GeneID" id="30198879"/>
    <dbReference type="OrthoDB" id="2250022at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071920">
        <property type="term" value="C:cleavage body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990342">
        <property type="term" value="C:heterochromatin island"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="GO" id="GO:1990251">
        <property type="term" value="C:nuclear exosome focus"/>
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        <property type="term" value="C:nucleolar peripheral inclusion body"/>
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        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:histone mRNA catabolic process"/>
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        <property type="term" value="P:nuclear mRNA surveillance of meiosis-specific transcripts"/>
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        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
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        <property type="entry name" value="POLYMYOSITIS/SCLERODERMA AUTOANTIGEN-RELATED"/>
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    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0271">Exosome</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
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</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5H0</accession>
    <name>A0A1E3P5H0_WICAA</name>
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        <recommendedName>
            <fullName evidence="5">Transcription factor spt8 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_29248</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="F:TBP-class protein binding"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Beta-prop_SPT8"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
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        <property type="entry name" value="AFR153WP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF23798">
        <property type="entry name" value="Beta-prop_SPT8"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="6"/>
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        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Transcription factor spt8 beta-propeller" evidence="5">
        <location>
            <begin position="59"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="191"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="312"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="251"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="259"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="290"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="485" mass="54083" checksum="1573C8004FF7A697" modified="2017-01-18" version="1">MEDANEDGNVNIEVEEPKSTRQVDDQQEDNEAPPPQDGKNQLESFYNQMRFKAQLANSYDVNPFVAIPYSSPINSIALSASSKWLFTGGDDGFIRKYDFFSSIDGEVQLTAAQKHSLVDSITNGGALQGYWENEIPVKRSMLTKDSYEPKLSPVFSLAIENRCLWLLSGLSNGGINMQSIRHNEGTIVHHFAGHQDTVSALKLNQAQNKFISGSWDKLINSWDLNSGKIINTFKDATGQISSLEFRPAGGVDIEAQPINEDDDMDSLFGDEEEEQQNGKQQRSTKQQRKSSSSSTVPSTTTTTTTTTHQGLDENIFMSSSIDGSINIWDLRTNQQVLRLKGTKHSPPWCVSSCWSQDGNFIYAGRRNSTVDEISLRMPLDSNNEIKVSKTLKFPQVSGAVTVVRPLPNGHHILCGSYDNIRIYDLRLYEESHKKTPFLIVPGHHGGTLSDVQFDPTYKYMISASGNRGWQGSSTETVFIYDVELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5W5</accession>
    <name>A0A1E3P5W5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_12734</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039925.1">
        <property type="nucleotide sequence ID" value="XM_019180542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5W5"/>
    <dbReference type="STRING" id="683960.A0A1E3P5W5"/>
    <dbReference type="GeneID" id="30197788"/>
    <dbReference type="OrthoDB" id="10250725at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08217">
        <property type="entry name" value="STKc_Nek2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF10">
        <property type="entry name" value="NIMA-RELATED KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="7"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="289"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ60718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="375" mass="44233" checksum="FB6AABAF213A9E6B" modified="2017-01-18" version="1" fragment="single">PNIQEEYEVLELIGRGSFGCVRKVRRVRDGRLLVRKEIPYGHMNPKERTQLISEFRILSELNHPNIVQYVHHAHIPEQHMLYLYMEYCDGGDLSFIIKNYRNSNEYLPEGIIWNIFTQILMALYRCHYGVNSPLVTNIYEEMECPIITDNTRVVIHRDIKPDNIFLSDGKFKLGDFGLAKSLEHEIEFATTCVGTPYYMSPEILLDKPYTPLCDIWSLGCVIYELCALHPPFQAKTHLQLQKKIQDGVYPDIPEHYSASLRRIISYCIQTDLNQRAPTFELLQHLNFKIQMKDLQLNNYELNLKKFEEELMMKENELIKAKQSLNEELDYQRKLIEQEVEEIRINYQNEFHYVVDKEVKARLKQMQSGVSTPRPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6D0</accession>
    <name>A0A1E3P6D0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosome biogenesis protein RLP7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_61467</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biogenesis of the 60S ribosomal subunit. May act as a specificity factor that binds precursor rRNAs and tethers the enzymes that carry out the early 5' to 3' exonucleolytic reactions that generate the mature rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040115.1">
        <property type="nucleotide sequence ID" value="XM_019183183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6D0"/>
    <dbReference type="STRING" id="683960.A0A1E3P6D0"/>
    <dbReference type="GeneID" id="30200429"/>
    <dbReference type="OrthoDB" id="28644at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000465">
        <property type="term" value="P:exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018038">
        <property type="entry name" value="Ribosomal_uL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF26">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN RLP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
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        <property type="entry name" value="RIBOSOMAL_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="9">
        <location>
            <begin position="109"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ60908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="289" mass="33218" checksum="42EA156225B3442A" modified="2017-01-18" version="1">MSELNSNPEILLRKRKNADRLRIEKQENARKRQEEISRKKNQRKQKFVRAETLVAKSLATKRESERIKRVSKIERVNAISDSQNRQYIEKLNPEGVKSKELYSGKPTLYFIVRISGPHGVKIPTKVHKILNLLRLNHINTGVFIKLTESIYPLIKLISPYTVIGKPSLQSVRQLIQKRATINVKDQESDQVKSVKLNDNNVVEERLGDEGIICIEDIIHEIVSLGENFKTVSYFLNPFELNHDIVGYGPLAKLKKLEKREAKKQNKTFSNSGSAPILEIDIDEFVAQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6H1</accession>
    <name>A0A1E3P6H1_WICAA</name>
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        <recommendedName>
            <fullName evidence="6">Cytochrome c oxidase-assembly factor COX23, mitochondrial</fullName>
        </recommendedName>
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    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_28877</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
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    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the assembly of cytochrome c oxidase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the COX23 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019181547.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6H1"/>
    <dbReference type="STRING" id="683960.A0A1E3P6H1"/>
    <dbReference type="GeneID" id="30198793"/>
    <dbReference type="OrthoDB" id="9971592at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="CytochromE C oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010625">
        <property type="entry name" value="CHCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051040">
        <property type="entry name" value="COX23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46811">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46811:SF1">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 7"/>
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    </dbReference>
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        <property type="entry name" value="CHCH"/>
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    </dbReference>
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        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CHCH"/>
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    </dbReference>
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    <feature type="domain" description="CHCH" evidence="7">
        <location>
            <begin position="50"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="99" mass="11830" checksum="5505446220D9DA58" modified="2017-01-18" version="1">MDPAIAKEKGKIDFTKGSVNEYKFYPDNPTSEYNKSKFASKEPSKYYDPCAQSAQMSVRCLEDNNFDRDMCHEYFKAYRECKKEWLRQRRQDNFNGKGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6K0</accession>
    <name>A0A1E3P6K0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar-sorting protein SNF8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_40689</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040258.1">
        <property type="nucleotide sequence ID" value="XM_019182556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6K0"/>
    <dbReference type="STRING" id="683960.A0A1E3P6K0"/>
    <dbReference type="GeneID" id="30199802"/>
    <dbReference type="OrthoDB" id="283883at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
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    <dbReference type="InterPro" id="IPR016689">
        <property type="entry name" value="ESCRT-2_cplx_Snf8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040608">
        <property type="entry name" value="Snf8/Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12806">
        <property type="entry name" value="EAP30 SUBUNIT OF ELL COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12806:SF0">
        <property type="entry name" value="VACUOLAR-SORTING PROTEIN SNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04157">
        <property type="entry name" value="EAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="26481" checksum="EEE84FAC596F83FC" modified="2017-01-18" version="1">MKKVGLAAFDDNKLRQYSELGQSLLQKKTNELTTQLQLFKSILLNFLKEHNEELSKDITLRNEFIKICQSVGIDPLQIYDKHDSKMNEFYFELCVRIIEFSNSMKDMNGGLLPVNEFLSSFKDLNITFKDVEISIGLLSALNDELKIIKIGSKHYIKNFNIELTNDQNMILETVGNIGFISVAILRDNFGWKSYRSQSILNEMISSGYLWLDDNNGELLYWDPAWLTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6V6</accession>
    <name>A0A1E3P6V6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CAF1B/HIR1 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_28335</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat HIR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040366.1">
        <property type="nucleotide sequence ID" value="XM_019181476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6V6"/>
    <dbReference type="STRING" id="683960.A0A1E3P6V6"/>
    <dbReference type="GeneID" id="30198722"/>
    <dbReference type="OrthoDB" id="71227at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033186">
        <property type="term" value="C:CAF-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055410">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045145">
        <property type="entry name" value="PTHR15271"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15271">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15271:SF4">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24105">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CAF1B/HIR1 beta-propeller" evidence="11">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="10"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="63"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="122"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="164"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="CAF1B/HIR1 beta-propeller" evidence="11">
        <location>
            <begin position="302"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="223"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="483"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="517"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="519"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="540"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ61159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="588" mass="65059" checksum="28A83C94131E6C44" modified="2017-01-18" version="1">MDASILSVHWHENSEPIYSVHFQPNREGNHSERLVTGGGDNNVRLWKINNSTDGKRVEYLSTLAKHTQAVNVVRFDPKGEILATAGDDGTVLLWTLSESIVREFGQEMDEDVQESWILRHACRSSTSEVYDLAWSPDSKYIITGSTDNVSRIYDASNGQQISQIAEHNHFVQGVAWDPLGRYIATQSADRSVHIYLLNIVSSKDQTLSPTVFFKISRTELPTRSSTTSTLMGPPTPAKSHSEEDSTSRGSTVEIPESSAKPQATVASSASPTTASSSSMKSPSPSPLPAVRSQNAPLLNFNNLKNSMLYHTENLESFFRRLSFSPDGNFLFTPSGIFKNSVNSEDSKEEIINTVYIYSRSGLNKPPIAHLPGLIRPALAIAFSPVFYELDKNEAKGPIFQLPYKMVYAVATQDSVIVYDTQHSAPLGTVSNIHYRTLTDLAFNSDGNSIVISAADGFCSLITFEPSTLGTKLSVDYKSLLPVSKENNEHNNKMQSPSQVKKRPNNGIMEITGLVKKKVKPEEPKTTEKVERISVQPSIKPEQEQKPKENEIITIEDNAEPQMIHTPPVKQVKRVAPILVEDKTSETPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P774</accession>
    <name>A0A1E3P774_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_77918</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040486.1">
        <property type="nucleotide sequence ID" value="XM_019184635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P774"/>
    <dbReference type="STRING" id="683960.A0A1E3P774"/>
    <dbReference type="GeneID" id="30201881"/>
    <dbReference type="OrthoDB" id="2133190at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052099">
        <property type="entry name" value="Regulatory_TF_Diverse"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336">
        <property type="entry name" value="TRANSCRIPTION FACTOR HMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR HMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="360"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="BHLH" evidence="4">
        <location>
            <begin position="177"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="277"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="278"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="721" mass="81377" checksum="9BF170E974DD3541" modified="2017-01-18" version="1">MSYAFDDYDAFLASFNNNNYKAPNNNMNQNQQISIAHDPSLTNSITSTEEEGMLDPYYDFGTTANGANNNQNNNNNNINGNFYLSPDSIIGTSNSSSPNEFQLNNEFNSLDGAAVNAGGDFNYDDGFMDQNQIKKESISSTASSSTSPVPVTQQHNTRVKLSNAVPHSTIGKRGPKKEKSSHNMIEKRYRTNINDKINALRDAVPALRVLVDSGDDEVDDDELDGLQPAKKLNKATILSKATEYIKHLEHKNEVLKKENDTLKHRLYGISNGNITPESSVSSYSSETVQNQNDGNRDGNRGGAHQNQTSYTNKFLLGGLACMVGAGLNDDLNHVDSTRSLFALPVFSFNQQGSQVVGKPLLILFKIGLILCVLLNFVFPAIFQSKKKVGVETSEVVDTEDIEDVRNLSKFSIWKTFKSTKDSSNNDYVKNSIIRCLKLKIEAMNQNFINQKLINFYVEHLWNYSKQVQLDSSESNFEDLNLILSLNNNETIHNEEYLKKLLNFEVKSNLEAELEDEEFTNNYSVIHILYEMINETKTNSILAKFMNSTINSSFQPTETKTLSEYINVELTTLFNPTDENISKLKENLSKLNINGVKDDEIFIMYASIIQNLIFIKKDFKSAQKWFKKLDLKLINKFNLLGFTSLYIIILSMTKYPELYQDDQDIILQIEEISGILRIWLGNTSGSSLKFDKRSQLIEFFVNVNLKINGLESLNGNLRTVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7B5</accession>
    <name>A0A1E3P7B5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_77955</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040528.1">
        <property type="nucleotide sequence ID" value="XM_019184641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7B5"/>
    <dbReference type="STRING" id="683960.A0A1E3P7B5"/>
    <dbReference type="GeneID" id="30201887"/>
    <dbReference type="OrthoDB" id="4159781at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF12">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="17"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="790"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="802"/>
            <end position="819"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="879" mass="99833" checksum="EE94F13DE3E8A09B" modified="2017-01-18" version="1">MDNAGKVKKTRYRVPTVCTVCRRRKMKCDKAKPHCSSCVKNNTTNLCVYEEQPWAANTDQQKLKDQIFQLQQQNNELKRLLSFNARNSQNFSSGSSLSSESPFSSNFNFILPDQPVNDPVMELTEDFDLLLLKESKMAHYGSTSYMAAVSKDPILRDIMSRYLEQQKMGINFDKFFLYDQQNFATIEDNCHVVGMHTANGTSVFETNLNKDHQELLKLVTEILPPLHIIEALVDHFFKETYVFIPFVDEDAFRQNMKEMITTNESGKIELRLKNFTLLATVSLLLCVLRFSFISLPIQKPEQVKDLNVKKILKSGVQIGPNFIEYAKACMANASVLRKPTLKHITALLVLRLYRFYAPEDGDESTDSTIFLALIVQMAKMHGLHRDPSRFSMVTSHSTITSWRKIWYQLMYLDAVQALQFGCPLLIDDEYDTLAPAANHSDSPLEKSCINDLKNQHEVTLLVRDLVKSCSKIKTHPKRSELEMKIKNVEFLLMKHRSLEDLTDFENESSQSFITKAGKIREMTCKLQLYAVHYISYYLLLLTCEPHEIRLIQRFSNAVSESALILFRLCHEYSKDPAKFNQSLYFESFLSASIFDSSKRLLQALSSLCVRDLSNMYSFQIALDNFVLDDAKGLTEWLQPPGTELTVGEQLLIRIEEFTQACARLSNKYYICWRITYVQKLFSDYLDYHYPGRLQELHEKAKRCENAGKFGVQTDGAIKDVEMGFPEIDPITNNYWGQFVDPGNQSQFDYLAPDLNKFDGIVDPFLSDAYLGGNFNFQDFDSIFAATTAGSGSGSGSTGVSSNSIPTDEVSPQSDSSNQTDKSKNVENFIYGPDGNLQENMSMKDITMSPAVNDHDLAMQVARSMFGGNGGYSSGSNGPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7G8</accession>
    <name>A0A1E3P7G8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_51580</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fungal Na(+)/H(+) exchanger family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040034.1">
        <property type="nucleotide sequence ID" value="XM_019182672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7G8"/>
    <dbReference type="STRING" id="683960.A0A1E3P7G8"/>
    <dbReference type="GeneID" id="30199918"/>
    <dbReference type="OrthoDB" id="5327978at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000015">
        <property type="entry name" value="Na(+)/H(+) antiporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004712">
        <property type="entry name" value="Na+/H+_antiporter_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382:SF4">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="75"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="414"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="13">
        <location>
            <begin position="25"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="468"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ60827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="487" mass="54084" checksum="1337386BA4627859" modified="2017-01-18" version="1">MAVWDQLEVDQAHKAYAIVGVFSTIFCLLSLVLKERLFVGEAFLSCVTGIIVGPHALNLINPLEWTNSDYLTQEISRIVLIIQIFAVSVELPKKYMKVNVRSVVVLLLFTMILGWLVTGLFAWVLFPKFSFIDGLAITAAITATDPVLAAAIVGKGKFGERIPASLRHLLSAESGANDGLAFPFVLLAVDILTNRGKPGVIVRDWFCIAILYECVFGVILGIIIGYSGRKAIEFCENKKFIDRESLLIFYILLSLMCTGFGSILGVDDLLVSFAAGAAFAWDGWFFKKSHGQHISNSIDLLLNVGYFLYFGTIIPWQDFNNKELGLHWWRLVTLGLVIIFLRRIPAALLAKPFVPAIKTWKEALFVGHFGPVGVGGIFCSIITKDAIEKHLTQGEVPMREINSSAPYYMVLRCIWPIVCFIVVTSIVIHGFSVTAMVFYSKFQNYDFKESKSSSPTMVSSVEITDEDGNKALNEPTSNSNLHLRRVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7H1</accession>
    <name>A0A1E3P7H1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_83421</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040437.1">
        <property type="nucleotide sequence ID" value="XM_019185540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7H1"/>
    <dbReference type="STRING" id="683960.A0A1E3P7H1"/>
    <dbReference type="GeneID" id="30202786"/>
    <dbReference type="OrthoDB" id="2018133at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004165">
        <property type="term" value="F:delta(3)-delta(2)-enoyl-CoA isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000048">
        <property type="entry name" value="3,2-trans-enoyl-CoA isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051053">
        <property type="entry name" value="ECH/Chromodomain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684:SF1">
        <property type="entry name" value="ENOYL-COA DELTA ISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="273" mass="30697" checksum="0C97161CC1285F4A" modified="2017-01-18" version="1">MAEGKDILYEVRDKFAIITLNLPKGLNALNNDQYLLLGILVERADRDPNTVFTFIRSTGRYFSAGANVKDVNLVIEENKSELEDKNYWLSRFTARNAYLTTIFHDHSKVLIAALNGPVVGLSSALVLLCDFVYAINDDVFLLTPFANLGLVSEGAAAATLVQRLGLSTANEALLLSRPIGAKKMYERGLINKIYNLKDTSKFNDLVLEEFWSNSKNLEFSSILDIKKLIKANYLQDLQSTNAKEVIGGMQKWVQNIPQKKFKSIAKKQMKHKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7L6</accession>
    <name>A0A1E3P7L6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DNA polymerase epsilon catalytic subunit</fullName>
            <ecNumber evidence="17">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">WICANDRAFT_52552</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">DNA polymerase II participates in chromosomal DNA replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="17"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040626.1">
        <property type="nucleotide sequence ID" value="XM_019182690.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P7L6"/>
    <dbReference type="GeneID" id="30199936"/>
    <dbReference type="OrthoDB" id="10060449at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032183">
        <property type="term" value="F:SUMO binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035822">
        <property type="term" value="P:gene conversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05779">
        <property type="entry name" value="DNA_polB_epsilon_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05535">
        <property type="entry name" value="POLBc_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000002">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000010">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1600.10:FF:000006">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013697">
        <property type="entry name" value="DNA_pol_e_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029703">
        <property type="entry name" value="POL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055191">
        <property type="entry name" value="POL2_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054475">
        <property type="entry name" value="Znf-DPOE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670:SF0">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08490">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22634">
        <property type="entry name" value="POL2_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22912">
        <property type="entry name" value="zf-DPOE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23250">
        <property type="entry name" value="zf_DPOE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01159">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 17" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 17" id="KW-0235">DNA replication</keyword>
    <keyword evidence="14 17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 17" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="12 17" id="KW-0408">Iron</keyword>
    <keyword evidence="13 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 17" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase epsilon catalytic subunit A C-terminal" evidence="19">
        <location>
            <begin position="1505"/>
            <end position="1890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1212"/>
            <end position="1239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU365029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ61419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="2193" mass="252922" checksum="6C0646DD10C01216" modified="2017-01-18" version="1">MGAPANGKFKGSTSARYVKNTINKRDDYVPNGSSNYKAPRDETRSKLDQVKKNDQMDTLMGFERFESGPTRTGWLTNMHATTIPSKDYISGVAGVDYYFLDEEGGTFKATIQYDPYFLVACTPGSEAEVEELLKKKLEQYLKSVSKIEKDDLAIPNHLVGIKRSLLRLNFPNSNDLFEAKRIIQPIVKENQTKKDTRDLYSAVNNNYTDEFSTNAKQEVLVDASEYIEDIREHDVPYCTRVSIDKNLRVGKWYKVSADQGNIEVEEIPERIDFGDAVILAFDIETTKRPLKFPDSAIDQVMMISYMIDGDGFLITNREIISEDIDDFEYTPKAEYPGLFTVFNEADEKATLQRFFEHIREAKPTIIATFNGDFFDWPFVEARAAVHDLNMFDEIGFAPDSEGEYKSTYCAHMDCFRWVKRDSYLPQGSQGLKAVTTSKLGYNPIELDPELMTPYAYSKPQLLSEYSVSDAVATYYLYKKIVNNFIISLGIIVPLNPDELLRKGTGTLCEMLLMVQAYENGILLPNKHVDPLERFYDGHLLESETYVGGHVESLEAGVFRSDLESEFKIDPTAIDEILGDLENALKFSIVVEGKQKLEDVTNFDEVYNDVKQQLLELRDNPKRSEKPLIYHVDVASMYPNIMCSNRLQPDSMKSEKDCAACDFNRPGKTCDRRMTWAWRGEFYPAKMDEYGMIKRSLQNETFPPKKPYLPRRTFEDLTYQEQVAQIKKRLQDYSRKVYHKVKQTETIERESIICQRENPFYVDTVRSFRDRRYDFKGLAKVWKKKSDTKDRLEKEEAQKRVVYYESLQLAFKVILNSFYGYVMRKGSRWYSIEMAGITCLTGATIIQMARALIDRLGRPLELDTDGIWCILPKSFPEDFEFTLKNGKKVFVSYPCSMLNYLVHEKFTNHQYQTLVDPVTKRYETHSDNSIFFEVDGPYKAMILPTSKEEGKGLKKRYAVFNDDGTLAELKGFELKRRGELQLIKNFQSDIFKLFLEGDTLEGCYAAVANVANRWLDVLDTKGRMLEDEDLIELICENKSMSKSLEEYEGQKSTSITTARRLGEFLGAEMVKDKGLAVKYIISERPLNAPVTERAIPVAIFSSDITVKRNFLRRWLLDPSLDDFDARNIIDWNYYKERLESVIQKIITIPAALQDVENPVSRVAHPDWLQKRISVSKDKKKQSSLATFFTKTKENPVDVMKDIEDIGKEEAAEGKRPLVGKVTSKKRKNQHTETSDPTEEEVNLLNGACPSIDEDYVSFLEYQKAKWKVQKKSRERRKALFGESSESSYRSTVGNMVRHQAESFAGSTWQILQYKLDTNPGDIRAFVLIDGKIQSFIFHIPRKLYLSFRSTALPGGTIPSCEVEKVSSILPNGHKASNLFRLTMPESVYQDELNKAGSILQDPNVLGLYEAQVEPTERAIMELGNSVTFNSNKIGSLGKGLKNGFELKSLSMTDTHHYLQKFTMPTAYLFHMISNEYEIYSLFKSWDKKVMVFVLKPSRNAQPISGNLEKIYKEIYEAKKEKFSKLYDIIDYQEELEFETQYFEELPKLFKKLNSFINKINDESPSKPLFVLQSPISNKLSKSFKVLTDFPVIHMNVGEINAPAIGWQTIVLKRICNHYLSLASWIKHLLSLAKYGNIPLCNLKTDNIGYLIDIEYARRLKKNDIVLWWSPNPVPDYGGVQKDMILMNNENYEFPIINNPEIYETVCLEIDIDNLTINTVLTSSLINEAEGTDLADMELIFDENNGTSTFAEDSFSGASLAVLRTMVKDWWDDAVKDNVNADSMVHTFVSWVQDNKSFLYDSSLKYHVHNLTKKALLQLVGEFSKLGSSVVFANRSKIILKTSKVSVENSYAYAQYIVKAVRSRPLFNYLDLQIDKYWDLLIWMDQHNFGGRACLDISEKEEQELIGLSKWQIKQYLPRLYQPEFEDWIIIFLDALIKEKNKMGNDFSTPRVTQINHILKKQQQSTKSQEEDIQNGICEAFRKPLLKRVKQLLRNQTEAILDPDLLEEYEFPKLPGSSTNMKNPTLELVKYLCAIFSLSKKRNLEIRLLRKELLNIFEVREFSKEAVFYNPSNTLKIAGVLCEYCHHIRDIDFCRDDEKILWKCEKCDKNYNKATLEESLIEGLEKLVTNYFIQDVKCEKCNRVKESDMTDYCPCSGRFVESQTKQEVADKFATYESIGEFYDLRLLRDFIDQVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7P6</accession>
    <name>A0A1E3P7P6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Eukaryotic translation initiation factor 4E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_29498</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040139.1">
        <property type="nucleotide sequence ID" value="XM_019181617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7P6"/>
    <dbReference type="STRING" id="683960.A0A1E3P7P6"/>
    <dbReference type="GeneID" id="30198863"/>
    <dbReference type="OrthoDB" id="590761at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019770">
        <property type="entry name" value="TIF_eIF_4E_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF8">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00813">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="24286" checksum="805CCE1348F638A9" modified="2017-01-18" version="1">MSEELNKATEGLSLDAPAAVNADTTILSDKEFTVKHPLNSEWTLWYTKPPSDPKESWADLLKPVINFSSVEEFWGIFNSIPQASDLPLKADYHLFRNGIRPEWEDSANAEGGKWSFQFKNKKDTNINELWLRTLLSVIGETIEDDENEVNGAVLNVRKFAFRVALWTKSTDKTKLLNIGMKFRKVLKLPDTEQIEFVGHKDSENKNSKPLMSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7X0</accession>
    <name>A0A1E3P7X0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_12114</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NFYB/HAP3 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040726.1">
        <property type="nucleotide sequence ID" value="XM_019180522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7X0"/>
    <dbReference type="STRING" id="683960.A0A1E3P7X0"/>
    <dbReference type="GeneID" id="30197768"/>
    <dbReference type="OrthoDB" id="386949at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22907">
        <property type="entry name" value="HFD_NFYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000099">
        <property type="entry name" value="nuclear transcription factor Y subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027113">
        <property type="entry name" value="Transc_fact_NFYB/HAP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064">
        <property type="entry name" value="CCAAT-BINDING TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064:SF9">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00615">
        <property type="entry name" value="CCAATSUBUNTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="5">
        <location>
            <begin position="12"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="105" mass="12008" checksum="424EE8F2CD229FB8" modified="2017-01-18" version="1" fragment="single">LLKEYEIREQDRWLPLANVGRVMKTALPSHAKLSKESKECVQECVSEFISFITSGAVDKCQAEKRKTLNGEDILYAMNTLGFENYAETLKIYLAKYREVSLISTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7Y5</accession>
    <name>A0A1E3P7Y5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CTLH/CRA C-terminal to LisH motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_61596</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040239.1">
        <property type="nucleotide sequence ID" value="XM_019183208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7Y5"/>
    <dbReference type="STRING" id="683960.A0A1E3P7Y5"/>
    <dbReference type="GeneID" id="30200454"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTLH/CRA C-terminal to LisH motif" evidence="2">
        <location>
            <begin position="20"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="174" mass="20487" checksum="1220AE7317F781AF" modified="2017-01-18" version="1">MIRNNNDDSKNDDNNQELFVMNIIKFAQDKLTNKAIKNVKFMEELELTMTLLLYSNNKDLLPAKLNELFEFKLRRDIADLVNKSILVKDSNNLIDLNYKKKFYEVKHEDNDNNNGNNEVNNDADEISIDDNDEDDDGLNSHIIDSNLKKMIKLWIWSENKAFDKNLEYPRLNLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P847</accession>
    <name>A0A1E3P847_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_75791</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040788.1">
        <property type="nucleotide sequence ID" value="XM_019184261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P847"/>
    <dbReference type="STRING" id="683960.A0A1E3P847"/>
    <dbReference type="GeneID" id="30201507"/>
    <dbReference type="OrthoDB" id="3980623at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053157">
        <property type="entry name" value="Sterol_Uptake_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784:SF5">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="1">
        <location>
            <begin position="48"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="508" mass="59044" checksum="B1A7A53F57D57F8F" modified="2017-01-18" version="1">MSCDIKTIKQPSKPKRRNYKTLFSGPGLINFNNNETITKPKRKRSKNGCTNCKRLKIKCNEDYPECEYCIHTGKQCVYPLLKNTKPKIKGQSSEVIDIQSNLIKDVVPMPSILNKPLQKLNSVSFQLNISKFELQLLKCYIDFGGNFFLKDVSKESHTFWSEDVPKLWCCSDLVKYGLYSASSARLLANYDYDTSKEIYIENEDQNSYPIIPFTINLREQASRYAEKTFELINMYQMMFDDQYGGALNESEDILGQLIVANKLAVGSKVILPRPKHNQTFNKVEESLLFQLMSVTNKTFQEFSKYSTLLKDTKYACIFDPEAIPINQHKLLVENVNLGFIRYLKNYVNDKFDPSDDFKITYQSAISRFEYSCHQTIYFQNTISLFRALVYITRDSDFLDLLKNEDHIAMKIMFYSCCLNSIFHDKIYQRNGVHNEFLEFYVNYSKDKFNGSWEDEVDRNIYGWVQKRAGCNYGFDLNALKYVGKPVDKVPPGVLRISEIDTGIKSTQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P890</accession>
    <name>A0A1E3P890_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histone deacetylase interacting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_90480</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040369.1">
        <property type="nucleotide sequence ID" value="XM_019186237.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P890"/>
    <dbReference type="GeneID" id="30203483"/>
    <dbReference type="OrthoDB" id="10265969at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032221">
        <property type="term" value="C:Rpd3S complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061188">
        <property type="term" value="P:negative regulation of rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061186">
        <property type="term" value="P:negative regulation of silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016479">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044804">
        <property type="term" value="P:nucleophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070550">
        <property type="term" value="P:rDNA chromatin condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030174">
        <property type="term" value="P:regulation of DNA-templated DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000001">
        <property type="entry name" value="Paired amphipathic helix protein Sin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000002">
        <property type="entry name" value="Paired amphipathic helix protein SIN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000003">
        <property type="entry name" value="Paired amphipathic helix SIN3-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.11">
        <property type="entry name" value="Paired amphipathic helix"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013194">
        <property type="entry name" value="HDAC_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003822">
        <property type="entry name" value="PAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036600">
        <property type="entry name" value="PAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039774">
        <property type="entry name" value="Sin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031693">
        <property type="entry name" value="Sin3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346:SF0">
        <property type="entry name" value="SIN3A, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346">
        <property type="entry name" value="SIN3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02671">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08295">
        <property type="entry name" value="Sin3_corepress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16879">
        <property type="entry name" value="Sin3a_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00761">
        <property type="entry name" value="HDAC_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47762">
        <property type="entry name" value="PAH2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51477">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase interacting" evidence="9">
        <location>
            <begin position="629"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="79"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="226"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="446"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="925"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1362"/>
            <end position="1494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="92"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="237"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="271"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="329"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="446"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="934"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="958"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="981"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1362"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1387"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1437"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1455"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1469"/>
            <end position="1494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ61162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1494" mass="168982" checksum="640C221840C1F86F" modified="2017-01-18" version="1">MSNQEPWKGSDSSDANRPGIPSLQHRQGAPVLPPPSSLPSHSSLESGGFYSLPSINVGGLSNASHNFVLNPANNGINPPPLNFQVKSEGTLPSQSQAGSSATPTTSASSQQQPQPQQQQAHQAQNQAQQQQQSAPKQHSGPGTPMSAYRPLNVKDALSYLDQVKVQFQNRPDVYNHFLDIMKDFKSQSIDTPGVIDRVSTLFRGHPSLIQGFNTFLPPGYRIECSLDPSDPNPIRVTTPMGTTTRPETNSNIVFEQQRWPSHSSQSGIPHAAGQSAQGLQIQSQGAQAQGQQQPQQRQQPPSVSQQPPQQQPPQQLPVQAPNDPYSHYANPNQEQSTMSQLQAAANRGNSITQLGDRKSGGPVEFNHAISYVNKIKTRFANQPDIYKHFLEILQTYQREQKPIAEVYSQVTILFQNAPDLLDDFKQFLPDTNGQAANGSALLQQGSDCYQQQQQQQQQLQGQPQLPPVGNFSPPTAGKMKKFEVTNDQLANYDAGRMGNKDIPTSNLRGSISMAPKRKFIEETTPTLVPGVPEPVQPPHKTSNLIEEIGFFDKVKKAIGSKQSYNEFLKIVNLFNQELIDKDTLIERVEGFIGSHLDLFDWFKTFVGFEDKPYHIENITHKKHQLDLLLCKPYGPSYRKLPKAETYMPCSGRDEMCWEVLNDEWVGHPTWASEDSGFIAHRKNQYEEILFRIEEERHEYDFYMEANLRTIQTLETIANRISNMTPEEKASFKLPNGLGHTSQTIYKKVIRKVYNKDKGFEVIDALHDNPSVAVPIVLKRLKQKDEEWRRAHREWNKVWREMEQKVFFKSLDHLGLTFKQADKKLLTTKQLVSEISTIKSEQTNKRLHPLTPKAQKQLDYEFTDIEVLFDISKLVYVFLNSGSSYSASDKEKLHEFFKSFISLFFSIDSQITESALQQRLAQAANGVPNEKEGSDISAASTASPAPTSDSENSRKRHRDTNLLKDVLRKSKQLKSRPDETPENSISPSHDSVANGAAEFEDDSKNGDEDIDINEFDSGSDTWVQTSLGENRKAEAINDKRNIFNLFGNTNIYVFFRHLRVLYDRLLEVKNINEEVTNDIKSRSEVQFAKDLNLISNQLEDMGLQFAKYDAYSQLLAFSERVIEGDVEHQWFEESLRQAYRNRAYKLYTVDKVTQALVKHLHTIVSDSRTSEIMLLFEADRKSKTTSAKDQIIYRNNVRSHMSPDENMFRIEFVEDKHHVSIQYLALDDLTLTDHKTEEDKWNYYLTSYIIAHPTEGINSSDLRLPFLRSLITEDAEDNDLEGYTDSHMKIKITRDNYKLFFEPNSYDEFTRYSVFNHPSKDKNKGVDALSKIVDEKTSSVLGEKRFGGAKDVFDALVEGPEKFKEVSKAKEEEAAKEKEQEKSSKDDVDVDQTIEGGDETIPEVKKPVNPAIASVKESDVTVDADSTIPQDDTEVRGNDTTTEENVVTEDNGNTEDNNATDTTSIITDQQNEKLAEVKEESKEETDKDGDAKMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8C9</accession>
    <name>A0A1E3P8C9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_81903</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040868.1">
        <property type="nucleotide sequence ID" value="XM_019185308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8C9"/>
    <dbReference type="STRING" id="683960.A0A1E3P8C9"/>
    <dbReference type="GeneID" id="30202554"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000065">
        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF15">
        <property type="entry name" value="TRANSPORTER SEO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="325"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="395"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="452"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="486"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="564" mass="64986" checksum="587AD51FEDDE855A" modified="2017-01-18" version="1">MVGKDYSAWYWRYIPHERVVEDWAEDDKSISSAFEELEYRDEADRKWWKFFDEYEYRYNKQTRSQKSWWRFFDKNASSAEKKLVIKLDLLIVTFAIMSYWSKNLDQANISNAYVSGMKEDLNMQGNDYSNAQALFQAGTVIFQIVFMYLFPRVPLHLLFFGGDLIWSAVTFATAFIHTPQQLNVCRFFVGAGESGYFIGMHYLLGSWYKADELGRRGGLFYCGQMLGTLTSGLLQATIFQNLDGVHGRSGWRWMFLVDGLATCTIAFFALYLVPGTPESCCSLFLTDDDIRLARYRLKKANVTPPSKDPPPFFNKKLWIKVLLDWRIYVLGVLDYLFWNAVNTGFSNFALWLKSLKRYNTPHLNRLTSIPPALGIVWILLVCGSADILKSRAFAIFWAEIFVLMGNIILAKWDVAESAIWYAFMISYFGISVSSVIYGWLNDIMRYNAQERSIVLCAVNIFANQSTAWTLPLVFKTSTAPRYHQGYVFVAGHSAALMVWSYVTLFFYKRQERKDARSNGIVLYNSKTGDIPVEVKKHLDNASSSVSINSQEFANLITRKNFAEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8Y4</accession>
    <name>A0A1E3P8Y4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DnaJ homolog 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_27317</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041080.1">
        <property type="nucleotide sequence ID" value="XM_019181355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8Y4"/>
    <dbReference type="STRING" id="683960.A0A1E3P8Y4"/>
    <dbReference type="GeneID" id="30198601"/>
    <dbReference type="OrthoDB" id="10256793at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006458">
        <property type="term" value="P:'de novo' protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072655">
        <property type="term" value="P:establishment of protein localization to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000005">
        <property type="entry name" value="Chaperone protein dnaJ 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000001">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000053">
        <property type="entry name" value="Putative Mitochondrial DnaJ chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096:SF52">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="11">
        <location>
            <begin position="64"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="12">
        <location>
            <begin position="216"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="10">
        <location>
            <begin position="216"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ61873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="498" mass="52679" checksum="B6518CA181830723" modified="2017-01-18" version="1">MAKSVICSLHTINQNVGKLATRSFSTAARRPIPNSIYAQSLKSSSFFRNKKSFHATSRALAVQDPYKALGVDKSASASDIKKAYYKLAKKYHPDINKEDGAEKRFHDIQGAYEILSDAEKKQQYDQFGSAAFDQNGNSAYGGGGGNPFGGGSPFGNAQGFGGFGNINFEDLFGSAFGGAGRGRGGGRGGSSYVTEYRGDDIEVVKYLTFKEAIFGVQNTKVNYSVLDSCGTCKGSGLKEGKKKSTCSACGGTGSTVHYMQGGFQMASTCMSCEGSGVSINPSDACGSCRGDGVSQANKSTEVDLPPGLSDGMRLRVSGEGDAPKVTTGPGIRIQKGDLIIRMRVKPDPRFKVSGSDIICKTNIPYTTAALGGQVEIPTVDGPSVRLRVPSGTETGSTLSISGKGVPIRNNINNRGDLKIEFTVKIPKPDGAAQTALLEALADSIGDNNAKRTNIDWKASSNADSTSDKCSSDHPSNLKKIENFLSDAFKRITKNKDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P900</accession>
    <name>A0A1E3P900_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Rhomboid-type serine protease 2</fullName>
            <ecNumber evidence="4">3.4.21.105</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Rhomboid protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_59924</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Probable rhomboid-type serine protease that catalyzes intramembrane proteolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">cis-Golgi network membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="4"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041055.1">
        <property type="nucleotide sequence ID" value="XM_019182903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P900"/>
    <dbReference type="STRING" id="683960.A0A1E3P900"/>
    <dbReference type="GeneID" id="30200149"/>
    <dbReference type="OrthoDB" id="10257275at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066:SF1">
        <property type="entry name" value="RHOMBOID PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="14">
        <location>
            <begin position="58"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ61848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="278" mass="30492" checksum="284B539AF7B91585" modified="2017-01-18" version="1">MEANLKILRQYFPLGGDYPPALTIGLTIFLPFLYILNFFISINDHIALSPTDLFKLDLNRVSLYPLGHLSIFHVLMNVLALITPLSNFERRHGTVYTGVVLNLLAVSTAIPYCILGSVFIPNTKIIGASAWAFSFAGYFAYKESLIKPTFQITPSHSLPTLATPLIPLILIAIFVPGSSFFGHLFGLISGYALAYGYLAKLAPPSKVIEFIESKLDGLINLIPSMFIYYREVEAKHLREQTDYVSLIGPTETILPTTNNPTAPASNPTFAGQGHVLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9C0</accession>
    <name>A0A1E3P9C0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_82140</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041213.1">
        <property type="nucleotide sequence ID" value="XM_019185340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9C0"/>
    <dbReference type="STRING" id="683960.A0A1E3P9C0"/>
    <dbReference type="GeneID" id="30202586"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07920">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000237">
        <property type="entry name" value="Pumilio homolog 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033712">
        <property type="entry name" value="Pumilio_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF13">
        <property type="entry name" value="PUMILIO HOMOLOGY DOMAIN FAMILY MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="4">
        <location>
            <begin position="1"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="55"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="91"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="128"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="198"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="270"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="330"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="349"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="358" mass="39990" checksum="6E971C1761B99052" modified="2017-01-18" version="1">MGQRRRGEDASKFANARLADFHGEIYSLCKDQHGCRFLQKQLDLNAEAATIIFDETHQHVIELMVDPFGNYLVQKLLEKVNDEQRITLVRNASPQFVSIALDPHGTRALQKLVDCINTQQEAEIIVKALSADVVSLSRDLNGNHVIQKCLQRLTPEDSQFIFDAAAKNCLEIATHRHGCCVLQRCLDHGSNEQCAELSLVISKFTVALSLDAFGNYVVQYVLAKNEKDAIAKIIEFVRSEIIKLSLHKFGSNVIEKCLRVSSLSSQLIDQLLNSEDDLVKLLNDPYGNYVLQTSLDVAKEKQFEQLSSLLRPLLPQVRNTPHGRRISARLQHSNDHVPITPAPTADETPANSATTTSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9C7</accession>
    <name>A0A1E3P9C7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Autophagy-related protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_87132</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)</text>
            <dbReference type="Rhea" id="RHEA:38571"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:38895"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Preautophagosomal structure membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATG2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041222.1">
        <property type="nucleotide sequence ID" value="XM_019186093.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P9C7"/>
    <dbReference type="GeneID" id="30203339"/>
    <dbReference type="OrthoDB" id="18982at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061908">
        <property type="term" value="C:phagophore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061723">
        <property type="term" value="P:glycophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026849">
        <property type="entry name" value="ATG2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13190">
        <property type="entry name" value="AUTOPHAGY-RELATED 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13190:SF1">
        <property type="entry name" value="AUTOPHAGY-RELATED 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13329">
        <property type="entry name" value="ATG2_CAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="226"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1070"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1163"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1548"/>
            <end position="1599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1636"/>
            <end position="1655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1070"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1163"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1580"/>
            <end position="1593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1637"/>
            <end position="1649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ62015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1783" mass="199096" checksum="49E9E5C767D821A3" modified="2017-01-18" version="1">MAPQWMPQNLQKRLLLYILQQLSLFSEIDLPNLDVSLGSSSQVTLKDVEIDPDALQLPGIFLRDGKVSNLKLQLNMSGGVTIEGDGLELTMALASPNSSSFENSITEKSKFSLAQSTIDLAASVMNTGNDEEGLESLHDFQTPNQSFHESSNDSDKDQTSTLNNVMAKAVEAALARLQVTLTNISVRLIMDQATIDLLVEKVTFTTNDGVRSVDISDVTVIAVKPVSGPGEDQAKEDSPADKEQTTKEDRFSTLHTRRSKEDEEDDYEDNVSDQSDSDEDADNDEMMSSSILERPADLNSSLVYSKEEASSIYMSATSKVFTRRNNDDHVARVMAINHINVLFSGLSKLEDLTIEVDEVKIAATPVPNTLLYVLESLAILNLKDTIHRHSSLNTKLRQKKNISAKDVEESAKKLFESISANTIEISLVSSLMENGQFAKPEELRIIGKNFTLEQKDSDYMFGSLNKLSMLNNKDDILLQFDEDKTKNDIRFESKGKTSELTLLLSKNLEISLDQDTIQKFINITSLLPQILDSLHKLSTSKPIRKPSKSSVLFQNASTTINFKINETTTINACIYPISYDSTKGSLSFNKATLSRNDVPLVSLFKFDVDILAAEKQIRSFDNSGHEIVLTTPCFVKIDRIELNHEYPDLKTSSDSLTELLKEISFPVEEIRFKARKKTRISTNVMFQSRQSVKLQASIGVISLGLKNLHPRFGDVSGFLKNVTLLIYKDNQVQLHSMNVDLKRIFGSVKEAIVEPINIEDRSAPIFSAKIREPKNINLFLRNSVVEYYTKWLSIFDDSGLKDDSSMNSTENLILGPSQESDGGLNVKMDLVDCAIGLNPGRLSSKANLVIRNGTIDVKLERNIIVKSQLRSAVLVLIDDTRNILSLEDAKRWRNINRKPSWSQVSFLNSRGYSSVANINSLHLETTIKKDGRVNEKGGLDSMIEMDINADLLDIELCADSSQCLVQLINDLKQPVAINNDLKYRYKVPDDLNVFDDIDSETFKSTIDDLISPIIGGSGSNSEPLNIVDSYYDDNETVNNDEYRESSAKLSGSDILDKSLDELAIKDETLGNNPFFTGESNTDGAGSSSNTENKSDNSDSIILFNDGHFEDSLDYSINPIKTKNHPMSLSLYVNQISLKIYDGYDWKHTRKTINIAVKRLEKKAAEEKEKREESNGRPRRASSSSNGERNEGQDEIIGETLFDSIHVAFPINSDPSKLTAKINRDLQSKEPEIDDQESNGIDVGKNNIKKLKLRRSKFHKILIELHEVEAKFTIVSNNDPVSSSKLKNDRDYELLNEIDLKVADFEIIDNVPTSTWNKFVTSMKDTEREVGASMLSMNIKTIRPVLTLAATELLMDVHVLPLRLHVDQDTLELFTRFGEFKDVRFNLVDEFEDVIFIQKFQVNAVHIKLDYKPKKVDYAGLRSGHTTEFMNFFILDEADMILKKVKLYGVSGFPRLGTMLNGVWMPDIKSTQLSGVLAGLAPVRSIVQIGSGFKDLVVVPVKEYKKDGRVYRSFSKGVQHFTKNTTNELLKFGVKIAAGTQTLLEHTEQALGGNGASSRVSQEVGVRKRRTTSTNDIIYEEHEEDYNSEDEDEGPGQKNGLLASQLIGRSSLSTTPLQRSIYQPALESFVDLERDNAARSSSRHGGSSPRNGHKRDVLYVATSDFDQFEDDSEDESQRTISLYADQPTNLKEGFQLAYDSLGRHLLVAKDAIINAGTEIGDSGSAQDSTLAVVKATPVALIRPMIGATEAVSKALLGVTNQLDPEQRKYIDDKYKNFNKNNKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9D2</accession>
    <name>A0A1E3P9D2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD-specific glutamate dehydrogenase</fullName>
            <ecNumber evidence="4">1.4.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_98619</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">NAD(+)-dependent glutamate dehydrogenase which degrades glutamate to ammonia and alpha-ketoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-glutamate + NAD(+) + H2O = 2-oxoglutarate + NH4(+) + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15133"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.2" evidence="4"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041226.1">
        <property type="nucleotide sequence ID" value="XM_019186662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9D2"/>
    <dbReference type="STRING" id="683960.A0A1E3P9D2"/>
    <dbReference type="GeneID" id="30203908"/>
    <dbReference type="OrthoDB" id="184415at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056365">
        <property type="entry name" value="NAD-GDH_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016210">
        <property type="entry name" value="NAD-GDH_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055480">
        <property type="entry name" value="NAD-GDH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF24">
        <property type="entry name" value="NAD-SPECIFIC GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23147">
        <property type="entry name" value="GDH2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23152">
        <property type="entry name" value="GDH_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000184">
        <property type="entry name" value="GDH_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="5">
        <location>
            <begin position="651"/>
            <end position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1017" mass="115342" checksum="30ADBC357CDEEDC1" modified="2017-01-18" version="1">MTINNLTASPPPDISALSITSLKSGYKNLPFIGKETQFEKVLDLIDKFGFIPENLIESEAKWFYESLGIDDVYFERESAEGIVSHIHALYSAKLDAFARNDLDSPFIHHKRENQDHAVYFDTSIPGTDQFKLTNYEKRIDENYLDQLDKSYRVESFNAPLVLNNSQFDTNGSSSTVTKTVRAHFVYENTFQHDSIYDQTFLKIISPNTKQLYDEIIEQALKQTGPLIKHFPVENSDEHRIIIGYQKNSTKKYFSALSALINYYKLDQSRKYVENFSNGVTIISIYLKPTLETNPLSIYQLIKEASLLYTIPHGVFYESFASNEFSLQESVYAHTGVIFVTHFLNRLGPEYQSLLKLLDPKQSHQVAELLSSLKKRLRSETFTQSFIFEVFNRYKSIISQLFRSFADVHYIKSNLEKTLSYQRLSQIPPIKNEDEFNQLLLKNVFNENDALVLRALFIFNKSILKTNFFTPTKVAISFRLDPSFLPKFEYPELPYGLFFVVGSDFRGFHIRFRDIARGGIRVVRSRSLDAYEVNVRNLFDENYNLASTQQRKNKDIPEGGSKGVILLDLDAQENPRSGFEKYVDSLIDLLIKNDIPGIKEPIVDLYGKPEILFLGPDEGTAGFTDWATLHAKKRGAIWWKSFLTGKSPQLGGIPHDEYGMTSLSVRSYVQKIYETYNLQSTPIQKFQTGGPDGDLGSNEILLSTPNEQYVGIVDGSGVIGDPNGLNKDELKRLAKERKMINNFDKSLLSKDGFIVLVDDKDVQLPNGEVINNGVYFRNLFHLNLKKYFGHVDLFVPCGGRPAAIDIDNINNLIDENGKSVISYIVEGANLFITQNAKIEFEKAGAVVFKDASTNKGGVTSSSLEVLASLVLNDEQFLSKFKNGTDFYREYVQQVQKTIVSNAEKEAASLLKYKAKTGLSLPEASDSLSNEINKLSDLIAQSDDLWNDLNLRKTIFTQSIPSLLLQEAGSVDEVLKRLPDAYSRSLFATAISTRYIYEDHDPQNVIELLQFLKKLGSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9Q2</accession>
    <name>A0A1E3P9Q2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_60007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041141.1">
        <property type="nucleotide sequence ID" value="XM_019182919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9Q2"/>
    <dbReference type="STRING" id="683960.A0A1E3P9Q2"/>
    <dbReference type="GeneID" id="30200165"/>
    <dbReference type="OrthoDB" id="2887at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004512">
        <property type="term" value="F:inositol-3-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002587">
        <property type="entry name" value="Myo-inos-1-P_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11510">
        <property type="entry name" value="MYO-INOSITOL-1 PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07994">
        <property type="entry name" value="NAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="168" mass="18357" checksum="5CFA0D9E0AED01EE" modified="2017-01-18" version="1">MVSLKVSNDNVKYNSDNTVLTSSYTYQNSIVSKQGETYTVKPFTKDYEFQVDLKVPKVGLLLVGLGGNNGTTFVSAVESNKQKIVFNTKDGEIKSNYFGSVTQASTVKIGIDESGKDVYVPFNSILPLVDPNDLIVSGWDINGENLQNAAKRAKVLSYDLQSQLGSIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9Q7</accession>
    <name>A0A1E3P9Q7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein URE2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_86591</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays an important role in the cellular response to the nitrogen source.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040889.1">
        <property type="nucleotide sequence ID" value="XM_019186055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9Q7"/>
    <dbReference type="STRING" id="683960.A0A1E3P9Q7"/>
    <dbReference type="GeneID" id="30203301"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051219">
        <property type="term" value="F:phosphoprotein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10293">
        <property type="entry name" value="GST_C_Ure2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03048">
        <property type="entry name" value="GST_N_Ure2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000034">
        <property type="entry name" value="Transcriptional regulator URE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017298">
        <property type="entry name" value="Ure2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF3">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR URE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037861">
        <property type="entry name" value="Prion_URE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="5">
        <location>
            <begin position="65"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="6">
        <location>
            <begin position="158"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR037861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="308" mass="35004" checksum="D5AA88BEE88C7DC6" modified="2017-01-18" version="1">MSQREPAISNLSSALKNVNINQDLRNPNLPNASNNLQSSLLNNNSNLTSNSESRITKFFKNQPLEGFTLFSHRSAPNGFKVAIVLSELNLDYNTIFLDFNNGEQRAPEFVSINPNARVPALIDHNVDNLSIWESGSIILYLVNKMLKEFGECSLWSDNLSDQSQITSWVFFQTSGHAPMIGQALHFRYFHSQQVPSAVERYTDEVRRVYGVVEMALAERREALIMELDTENAAAYSAGTTPLSQSRFFDYPVWLVGERITIADLAFVPWNNVVDRIGIDIKVEFPEVYKWTKHMMRRPAVIRALRGDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9R6</accession>
    <name>A0A1E3P9R6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_75895</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the bZIP family. GCN4 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040899.1">
        <property type="nucleotide sequence ID" value="XM_019184284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9R6"/>
    <dbReference type="STRING" id="683960.A0A1E3P9R6"/>
    <dbReference type="GeneID" id="30201530"/>
    <dbReference type="OrthoDB" id="5419235at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036033">
        <property type="term" value="F:mediator complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010688">
        <property type="term" value="P:negative regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001080">
        <property type="term" value="P:nitrogen catabolite activation of transcription from RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045899">
        <property type="term" value="P:positive regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990139">
        <property type="term" value="P:protein localization to nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12193">
        <property type="entry name" value="bZIP_GCN4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001491">
        <property type="entry name" value="Cross-pathway control protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050946">
        <property type="entry name" value="AP-1_TF_bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11462">
        <property type="entry name" value="JUN TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11462:SF35">
        <property type="entry name" value="TRANSCRIPTION FACTOR JRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="10">
        <location>
            <begin position="232"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="159"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="192"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="241"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ61692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="288" mass="31552" checksum="F32DE2FC89E7BABA" modified="2017-01-18" version="1">MSSSITTATTTSRSTIDSASALFELFESSLDSSAPMYQSFNQSGAGASGPITQDFELASKPRQVLGESVFNHFVKSQELNSEFPLGLDKIEVPELSPSIGTVTPLQLHSSIVESVFSPSIENSSPMFDDVELDTENWTSLFEPNELEIPPVVAAATTEIEQESTPIPEVESSSSTPAPPQFKRSSSEAELDFTSSKRQCSSSPSIETDHLGVVAYNRKQRSAPLSPIVVESDDPVAQKRARNTEAARRSRARKMERMNQLEDKVEGLVNRNQELENEVARLRALLAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9R8</accession>
    <name>A0A1E3P9R8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Ubiquitin-like modifier-activating enzyme ATG7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Autophagy-related protein 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_76318</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Autophagy is essential for maintenance of amino acid levels and protein synthesis under nitrogen starvation. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Preautophagosomal structure</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the ATG7 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041348.1">
        <property type="nucleotide sequence ID" value="XM_019184364.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9R8"/>
    <dbReference type="STRING" id="683960.A0A1E3P9R8"/>
    <dbReference type="GeneID" id="30201610"/>
    <dbReference type="OrthoDB" id="338614at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097632">
        <property type="term" value="C:extrinsic component of phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019778">
        <property type="term" value="F:Atg12 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019779">
        <property type="term" value="F:Atg8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032446">
        <property type="term" value="P:protein modification by small protein conjugation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000243">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.100">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.70">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006285">
        <property type="entry name" value="Atg7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032197">
        <property type="entry name" value="Atg7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042522">
        <property type="entry name" value="Atg7_N_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042523">
        <property type="entry name" value="Atg7_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01381">
        <property type="entry name" value="E1_like_apg7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF3">
        <property type="entry name" value="UBIQUITIN-LIKE MODIFIER-ACTIVATING ENZYME ATG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16420">
        <property type="entry name" value="ATG7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like modifier-activating enzyme Atg7 N-terminal" evidence="10">
        <location>
            <begin position="6"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="9">
        <location>
            <begin position="302"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="593"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR606285-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="620" mass="69215" checksum="584AFA27AC9DA977" modified="2017-01-18" version="1">MSQDSLKYSAPKSFVDSSFFQKLSNLKLDKFKLDSTKKDIHAVYNIKSIPQNESPAISLAAASFDEPNESLYPTNSVLFAPGFITNFNTIEEFKALDKAQFLKASGEAIIQSIENKEVLQNPSLLNRFDVISFADLKKFKFYYWVAFLTLSSHWSIKSKQPIPSISNEILQWISSSGATKKGYFIVKDNEALPLTDIDTTEDEITVGFVDSCVIEDRPSKFAQNFLTALSIYGFKKVHLNVYRLSGTSYTLDLENTDTEVGKVTGWERTAQGKLGPKLADLGSLIDPVQLSEQSVDLNLKLMKWRVAPEIDLDIIKDTKALLLGAGTLGSYVARALLGWGVRNVTFVDNGKISFSNPVRQPLFNFEDVGEKKALKAAESLKKIFPLVNANGYELEIPMAGHPVTNDEKQKQDFEMLDKLIELHDVVFLLTDSREARWLPTLIGNVKKKIVINAALGFDSYLVMRHGVEGNLGCYFCNDVVAPTDSLTDRTLDQMCTVTRPGIALLASSLAVELLVSILQHPEKHYANANTDTILGKLPHQLRGFLGNFDTKKLETPAYEYCSACSKPIQEGYEEGGWDFVARALNEPQYLENVSGLTEVQRQAEEAVADLEEDFSDEEWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9Z3</accession>
    <name>A0A1E3P9Z3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_25841</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041395.1">
        <property type="nucleotide sequence ID" value="XM_019181197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9Z3"/>
    <dbReference type="STRING" id="683960.A0A1E3P9Z3"/>
    <dbReference type="GeneID" id="30198443"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06609">
        <property type="entry name" value="STKc_MST3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000499">
        <property type="entry name" value="Serine/threonine-protein kinase KIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF27">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="9"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="278" mass="31340" checksum="21590758AD061E78" modified="2017-01-18" version="1" fragment="single">MRYLSANQFEVYEEIGKGGFGVVYKALDKVTKCPVAIKQIDLESTDDIDSLQKEIKILSQCKLKQITQYFGCFVKGYKLWIIMELLDGGSCLDMLVAGPIREKCIAVLFKELLIALDYLHSNGKIHRDIKAANILLNSQGDVKIADFGVSTQLSNNLSRRNTFVGSPYWMSPEVILEEEYNFKADIWSLGITALELATGKPPLSNIPPMKALFKIPENDPPILEGQEFSQDFKQFVALCLQKSPQNRPSAKRLMKTKFIQNAGKTSIVGDLIKRRQVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA01</accession>
    <name>A0A1E3PA01_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase III subunit RPC4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_60313</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041452.1">
        <property type="nucleotide sequence ID" value="XM_019182968.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA01"/>
    <dbReference type="STRING" id="683960.A0A1E3PA01"/>
    <dbReference type="GeneID" id="30200214"/>
    <dbReference type="OrthoDB" id="5836119at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007811">
        <property type="entry name" value="RPC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13408">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13408:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05132">
        <property type="entry name" value="RNA_pol_Rpc4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="149"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="168"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="193"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="206"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="395" mass="43321" checksum="A65C11A4F90FC958" modified="2017-01-18" version="1">MSDKIPTSSRLDSLSRRSTPSAKPGLKFKPKVVARRSKEERDAQAPVTAQESNKSDKSNRGNSARGRGGARGGRGGRGGRALAGTHLVQAGPLASGNILSDSTQSFTRSATASPTPDYLSNLVKREGVRSTSVGLDSDEEDDDPARINMSKEYKFDPEHTELFPVRPVRNEHVESGDHKANPSSAEYVRSESPESVSSEPSGSKTTVKEEPQEKDLNAVLHEKDTELQKKLNDLQLQSEFLSVDPTEAKQETERLNKDHRSIVKSIIEADNNPENFLFIQLPRVLPKFEENDEVKAAKAAQTISVKPEKEGDGDITMSEKKTEGEIDQLEGLVGKLRVHKSGKLTMKLGNVVMDVGKGSQTDFLQEVVLADENENRNAYLLGHLNEKVVVTPRFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA12</accession>
    <name>A0A1E3PA12_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone acetyltransferase GCN5</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Histone crotonyltransferase GCN5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_76317</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetyltransferase family. GCN5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041347.1">
        <property type="nucleotide sequence ID" value="XM_019184363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA12"/>
    <dbReference type="STRING" id="683960.A0A1E3PA12"/>
    <dbReference type="GeneID" id="30201609"/>
    <dbReference type="OrthoDB" id="1937912at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140671">
        <property type="term" value="C:ADA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036408">
        <property type="term" value="F:histone H3K14 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043993">
        <property type="term" value="F:histone H3K18 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043992">
        <property type="term" value="F:histone H3K9 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010515">
        <property type="term" value="P:negative regulation of induction of conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05509">
        <property type="entry name" value="Bromo_gcn5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000046">
        <property type="entry name" value="Histone acetyltransferase GCN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000004">
        <property type="entry name" value="Histone acetyltransferase KAT2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037800">
        <property type="entry name" value="GCN5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750">
        <property type="entry name" value="GH11602P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750:SF3">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8 15" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="18">
        <location>
            <begin position="97"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="17">
        <location>
            <begin position="341"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ62140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="436" mass="50987" checksum="71948E64B5E4BA39" modified="2017-01-18" version="1">MDENKRAAVDKHDDEPIAKRAKLEDEEKKIDIKPDDKKIKSEEGEEEEEEEAEEKEEAKDDDEAEEERKRTTTFKFDGQTYTFKERPSVLEEREGKIEFRVVNNDNTKENLMVLTGLKNIFQKQLPKMPREYIARLVYDRSHLSMAVVRKPLTVVGGITYRPFDSRGFAEIVFCAISSTEQVRGYGAHLMNHLKDYVRATSPIKYFLTYADNYAIGYFKKQGFTKEITLDKHIWMGYIKDYEGGTLMQCSMLPIIRYLDSAKILLLQKAAVQRKIRLVSKSDVVRSGLKHFKDLKNIKPIDPMTIPGLKESGWTPEMDELAQRPRRGPHHAFMQHLLTEIQNHTASWPFSTPVNKEEVPDYYEVIKEPMDLSTMESKLENDHYSTLEEFVYDARLIFNNCRKYNNETTTYFKNANKLEKFFNSKVKEVPEYSHLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA88</accession>
    <name>A0A1E3PA88_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_26174</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041069.1">
        <property type="nucleotide sequence ID" value="XM_019181234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA88"/>
    <dbReference type="STRING" id="683960.A0A1E3PA88"/>
    <dbReference type="GeneID" id="30198480"/>
    <dbReference type="OrthoDB" id="5121955at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052073">
        <property type="entry name" value="Amide_Lactam_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171">
        <property type="entry name" value="FARA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171:SF3">
        <property type="entry name" value="FARA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="6">
        <location>
            <begin position="74"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="482" mass="55948" checksum="0C20A099E699F659" modified="2017-01-18" version="1" fragment="single">QFITSTQPLAPILSRDSLLCLDGEILGLYCPWRDVYYEPRDRYGNKIDKELQTYLTKKGAFIVPQISEQRKLVQLYLDNLYPFYPVVQRDVINDLQSVPLILLNAMMLAGVRYDKSLKNTEFRIRAEEFRSRCALLQMIESNKVTLIQSYLLLSSNEEGPEGPSSSREYISKAVILSIELGLHNLATSEQFDLTKIDGTDKKTAFRLNYSRNLMARLFWTSFCCDRTTAATSCTAMLYNISDMIVDEPIVKDFDDGVHQTQDYELFKRWYTLTKLFERVLMKVYRPPGKRSVDDESLELDLLNWGIRNLNATLPVESKFIRFFKIVHAYCNLLYLRCKIGFLDLIEDNIQPNFESNSNPLSTAGMYYVTKFSKDVIENLTNGEDNIEHIINTHALLHVLVLLHLEMESKKQITQENSAEYTHQQFDQCLELLQQFRNKWWFSAAAFHLFKTLAEKQSTMKFSPTTLESIFINQGEFGEKLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAM6</accession>
    <name>A0A1E3PAM6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GTP-binding protein RHO3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_82466</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041625.1">
        <property type="nucleotide sequence ID" value="XM_019185383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAM6"/>
    <dbReference type="STRING" id="683960.A0A1E3PAM6"/>
    <dbReference type="GeneID" id="30202629"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030950">
        <property type="term" value="P:establishment or maintenance of actin cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045921">
        <property type="term" value="P:positive regulation of exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090338">
        <property type="term" value="P:positive regulation of formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010590">
        <property type="term" value="P:regulation of septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000780">
        <property type="entry name" value="Rho GTPase Rho3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="216" mass="23759" checksum="1BBE53B3053B8283" modified="2017-01-18" version="1">MPLCGSSKKPIQRKIVILGDGAAGKTSLLNVFTRGYFPQVYEPTVFENYVHDIFVDGKPIKLSLWDTAGQEEFDRLRSLSYSDTHTIMLCFAIDSKDSLENVQNKWVGEIADHCEGVKLVLVALKCDLRNDDGDDEDEDEHLNGVGSGSGRSNLITYEEGLEMAKKIGALRYLECSAKKNRGVNEAFTEAARCALTAKPKGANDDDEDQGGSCIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAQ4</accession>
    <name>A0A1E3PAQ4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_25406</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CYC8/SSN6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041702.1">
        <property type="nucleotide sequence ID" value="XM_019181140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAQ4"/>
    <dbReference type="STRING" id="683960.A0A1E3PAQ4"/>
    <dbReference type="GeneID" id="30198386"/>
    <dbReference type="OrthoDB" id="418911at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017053">
        <property type="term" value="C:transcription repressor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006972">
        <property type="term" value="P:hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000879">
        <property type="term" value="P:negative regulation of dipeptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042304">
        <property type="term" value="P:regulation of fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000403">
        <property type="entry name" value="General transcriptional repressor, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000078">
        <property type="entry name" value="Transcriptional corepressor Cyc8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051630">
        <property type="entry name" value="Corepressor-Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017:SF1">
        <property type="entry name" value="LD02225P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4 9" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="44"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="78"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="112"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="148"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="185"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="222"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="294"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="328"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="412"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="435" mass="48874" checksum="A76EA4937B546E7E" modified="2017-01-18" version="1" fragment="single">MSAQAVNNLVQQQQVPRQVQQQQAVAQAQQQLTAQEQELKNATAETWLAIGSLAESVGDADRAAAAYDATLRHSPNSAPALTSLANLYRARDMFAKAAELYQRALTAKPDAGETWGLLGHCYLMLDDLQRAYAAYQQALYHLSNPNVPKLWHGIGILYDRYGSLEYAEEAFGRVLELDPNFEKASEIYFRLGIIYKHEGKLAQALECFRYILPNPPAPLTQPDVWFQIGAVLEQQRDYNGAKDAYQKVLEANPKHAKVLQQLGCLYSQQGSNFTDPDIALRLLSQSLEIDPSDAHTWYHLGRVHMSRGDYTSAYDAFQQAVNRDARNPTFWCSIGVLYYQISQYRDALDAYTRAIRLNPYISEVWYDLGTLYETCNNQISDALDAYKQAATLDPNNPHIQARLEQLTKFQKEGGTNGALPPPPPSQQPRLPQGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAZ9</accession>
    <name>A0A1E3PAZ9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_103352</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041790.1">
        <property type="nucleotide sequence ID" value="XM_019180376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAZ9"/>
    <dbReference type="GeneID" id="30197622"/>
    <dbReference type="OrthoDB" id="10622319at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133760" evidence="2">
        <location>
            <begin position="24"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="276"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="434" mass="48343" checksum="37829FC38B5D14CF" modified="2017-01-18" version="1">MKFNSFYYSIILIPTIFATTALTDPPTKTITEVLTITYTPTETSKNNVVTKTLYETIHDGIESDQQTKPGDFAQTPSVPVAAPANGEFINSLLSDLKAHKKIAQNTNKTKVLVPGLNVESTSGVVPEANEQLKSQAGEDEFGYDADYFQSRLEPLISNPSEKDFHELNEEIDSQVSDEEDDDEEEEIYEESEDGEDFEDDVEVADEYDIDADVEISSPYHLDPNYRGTTNPKELEKPRPADATDDINQPDPTLDQNNPLEHQQPTIDEQQKDQDPENNNNPNEPQLVDEDLSNKYTHSNFTKPEPNEEGHPIPQPIGEHDPEEEEITKGKQVLDKEETDSLVIGNKTSGIPKKIKGNKLKSEDNDFENRFIQGSIIDGLDSKNQTSRTGKSSCRPLSTTLTKMNDDTSGGSMNYISLNSVLFIVGILSGFMTFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB17</accession>
    <name>A0A1E3PB17_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_82601</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FMO family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041816.1">
        <property type="nucleotide sequence ID" value="XM_019185407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB17"/>
    <dbReference type="STRING" id="683960.A0A1E3PB17"/>
    <dbReference type="GeneID" id="30202653"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000023">
        <property type="entry name" value="Dimethylaniline monooxygenase [N-oxide-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="498" mass="56029" checksum="4E14BB72DE39AA94" modified="2017-01-18" version="1">MTIDKFNINSIAVIGGGPSGIASVYEFLHTTKDGKSTVGGSNAENPAFTKVVGFEQKNKVGGSWAPSIEVPDLPEDELWGRNYYDADVIHPKVEIPKGLSNHSVESPLVTDGVNNDNQWRRSAVYPGLYTNVPRRFLRFSSIKYTPPSKLAEIDPLISYKEVGQVLDKFVDDNKLLDHFRLNTQVQDVQKDEEGKWLLTLRHSTPEGKDEWYTEKFDAVVIASGHYSVPFIPYIEGLNSAPKGTIVHSKSFRTVDQFKDKNVLVVGSSLSGIDIIQYIEPVVKSLTISRTPGKQEIFPWITKAATSFSNKPRISKIDGKTVFFEDGSKLEDVDKILFATGYHWHYPYIGEKFLELTKPGYANKATGGSRVKGLYRDTFNIEDPTLAFIGVQITSVQFHSFEAGAVGIAGVWSNSKRLPSKDAQKEWETKRLVETSDDYIFHYYPWDIVQKEFIDVLLEYAPDDRPYILEGEDFGDYPKALEAAERAFYDIKAGKYSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB48</accession>
    <name>A0A1E3PB48_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_66775</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041727.1">
        <property type="nucleotide sequence ID" value="XM_019184055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB48"/>
    <dbReference type="STRING" id="683960.A0A1E3PB48"/>
    <dbReference type="GeneID" id="30201301"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ABC transporter ATP-binding protein arb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000618">
        <property type="entry name" value="ATP-binding cassette (ABC) transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF15">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="66"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="377"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="594" mass="66294" checksum="5E9A73E71EF7E5B0" modified="2017-01-18" version="1">MAVSASKAKRDAKKAASGKKTKKDKVESVDAAADEIQKLKLQQDKEGISDRVTTGVLDSLETSRDIKLSSVSLLFHGKVLIQDSELELNYGRRYGLLGENGCGKSTLLNAIAAREFPIPEAIDIYLLNEPAEPSEFSALEYVVREAEHELKRLEDLVEDIIVKDGPESPVLEGIYERIDSMDPSTFESRAAVILIGLGFNSQTIKKKTKDMSGGWKMRVALAKALFVKPTLLLLDDPTAHLDLEACVWLEEYLKRFDRTLVLVSHSEDFLNGVCTNMIDMRLQKITGYGGNYDSYAKTRAEQETNQMKSYNKQQEEITHIKKFIASAGTYANLVRQAKSRQKILDKMEADGLIEKVVPDRVFSFRFPDIEKLPPPVLSFDDISFAYDGNKEHNLYEHLNFGVDMDSRIALVGPNGVGKSTLLKIMTGELQAQSGRVSRHTHIKVGVYSQHSADQLDLTKTALEFVRDKFSHISQDYQYWRGQLGRYGLSGEAQTAQMATLSEGQRSRVVFSLLALESPNILLLDEPTNGLDIPTIDSLADAINEFSGGVCVVSHDFRLLDKIAKDIFVVENRTATRWDGSIGDYKKKLAKNVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB85</accession>
    <name>A0A1E3PB85_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_76651</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041691.1">
        <property type="nucleotide sequence ID" value="XM_019184425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB85"/>
    <dbReference type="STRING" id="683960.A0A1E3PB85"/>
    <dbReference type="GeneID" id="30201671"/>
    <dbReference type="OrthoDB" id="5430106at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018857">
        <property type="entry name" value="TORC1_cplx_su_TCO89"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22794">
        <property type="entry name" value="THAP DOMAIN PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22794:SF2">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10452">
        <property type="entry name" value="TCO89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="478"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="313"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="540" mass="60365" checksum="668A30B2D70A4442" modified="2017-01-18" version="1">MPSGKQQQERKTKQFSARSHSRSASYGRNLNKLGRITSIEEIHQRPTLNRSKSTDGTLGRKNRSFTKLSSLQPLTRTRSHQPVHRGTGKAVLELHKEQDSSDDENDGTNKKNDSESEEEVDEFSDDEPAENIPEAPSQVQLSYKAPPQFSHNSVPKNSSFSSEPAEEPVQVDKLQESVENSLKLDPTRIETEASKLNLNDLSEKAGSSSQESKASAQPNQPQDVNQYKESLSQYYQNMILSQSTGAVRAFGDQPFMNSLVHDQIGQRNNKDTLQYIHSNDHISGNFGNMKSTSSSSNSLRQFTIHNLPNTHNSSNESSSAMSFRAKESGSNPGRLDSAAGTPNNFDQFLRTNNSNIETRTQQKLWLQRENSLLDLPSASGNNSNNPQLRRDFERVSREYVNVRRFSNPCVESVKRVSSQVKPLKPQSTGQMNLANTKHIKHVPSNDFQAKLFKLWIEGESPGNRRPNQPNIGFKYQQVPQSQRPPLLQTTSSSFSGMGNSLRSPAAPSTRAVDRANNSPENKRIDLAAIRLPEEHVQKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBG3</accession>
    <name>A0A1E3PBG3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_60368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041509.1">
        <property type="nucleotide sequence ID" value="XM_019182978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBG3"/>
    <dbReference type="STRING" id="683960.A0A1E3PBG3"/>
    <dbReference type="GeneID" id="30200224"/>
    <dbReference type="OrthoDB" id="8300214at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF38">
        <property type="entry name" value="ARSENICAL-RESISTANCE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="24"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="138" mass="15895" checksum="89FC3753FD1641B5" modified="2017-01-18" version="1">MDQLYSSCIKRLAPETLRNWIAEERDDIAVIDVRDSDYKYGKIPISIHIPSKGFGEDSIQNQLLSKYPLGDTSVYIFHCMRSQQRGPSSARKFLRYLLLKTECKDDLHKLPKVYILDGGLKKWNQVYGADPELMVGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC02</accession>
    <name>A0A1E3PC02_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Potassium channel tetramerisation-type BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_89117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042115.1">
        <property type="nucleotide sequence ID" value="XM_019186175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC02"/>
    <dbReference type="STRING" id="683960.A0A1E3PC02"/>
    <dbReference type="GeneID" id="30203421"/>
    <dbReference type="OrthoDB" id="9451547at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903293">
        <property type="term" value="C:phosphatase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903452">
        <property type="term" value="P:positive regulation of G1 to G0 transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="38219" checksum="B1F62F4B773C2059" modified="2017-01-18" version="1">MSNPDIITQVSQDAGPMRPEATTQMDEDADLNSIIKLNIRGKMFHITRDELMSLPESILLCLFPNGVFVDVDGNVITNLTEDDIVYVNFSPECFQYIVDSFGAAAQRLPPSSQPEIYDSYTTPDIEVLNEKPSIIVLREDLDYYVIPPRKGISPQGVRAIKSAAGSYLVSRNKVFEGLGYAPGKKLGPAEEHLLEMLCSSGFNVESDWGHRCSEPGKTVLTSLALVDLVHPEQQQEPESPSLNPVSSNNTKVSRRSRIVELAGNAARSASRSVSRNRSSSRTKSAGTSSKLLLFWRKPARKCWWSDDEVELKIPGLLHTDGLSDASIRLHIRRVWTLELSVIGVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC30</accession>
    <name>A0A1E3PC30_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cobalamin-independent methionine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Methionine synthase, vitamin-B12 independent isozyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_26555</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the vitamin-B12 independent methionine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.14" evidence="4"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042162.1">
        <property type="nucleotide sequence ID" value="XM_019181277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC30"/>
    <dbReference type="STRING" id="683960.A0A1E3PC30"/>
    <dbReference type="GeneID" id="30198523"/>
    <dbReference type="OrthoDB" id="1053771at2759"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00082"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019280">
        <property type="term" value="P:L-methionine biosynthetic process from L-homoserine via O-acetyl-L-homoserine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03312">
        <property type="entry name" value="CIMS_N_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.210:FF:000003">
        <property type="entry name" value="5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00172">
        <property type="entry name" value="Meth_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013215">
        <property type="entry name" value="Cbl-indep_Met_Synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006276">
        <property type="entry name" value="Cobalamin-indep_Met_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01371">
        <property type="entry name" value="met_syn_B12ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003556">
        <property type="entry name" value="PRK05222.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30519">
        <property type="entry name" value="5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08267">
        <property type="entry name" value="Meth_synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000382">
        <property type="entry name" value="MeTrfase_B12_ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Cobalamin-independent methionine synthase MetE N-terminal" evidence="17">
        <location>
            <begin position="4"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Cobalamin-independent methionine synthase MetE C-terminal/archaeal" evidence="16">
        <location>
            <begin position="439"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="705"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="444"/>
            <end position="446"/>
        </location>
        <ligand>
            <name>L-homocysteine</name>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="444"/>
            <end position="446"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="497"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="574"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="612"/>
        </location>
        <ligand>
            <name>L-homocysteine</name>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="612"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="655"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="657"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="677"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="737"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ62955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="769" mass="86320" checksum="3ACA29321FBD9D37" modified="2017-01-18" version="1">MVQSSVLGFPRIGAQRELKKTTEAYWSGKVSVEELFKVGKEIRANNWKLQKQAGVDVIASNDFSYYDQVLDLSLLFNAIPERYTKYELQPIDVLFAMGRGLQRKATETEKAVDVTALEMVKWFDSNYHYVRPTFSHSTEFKLNGQKPVDEYLEAKELGVETRPVLLGPVSYLFLGKADKDSLDLEPISLLEKILPVYSEILAKLATAGAKSVQIDEPILVLDLPENVKAAFKTAYEFFSKQEGLPALVLATYFGTIVPNLDAVKGLPVSAIHVDLVRNPEQLDQVLEILTPEQTLSAGVVDGRNIWKAVFSKASEIVNKAIEKLGKDRVIVATSSSLLHTPVDLNNETKLSDEIKDFFSFATQKLDEVVVIAKSASGEDVKEALEANAKSASARASSEITNDEAVQTRVGTIDDKLGRRAAPFTDRLKEQEPVFNLPLFPTTTIGSFPQTKDIRINRNKFNKGTITKEEYEKFIESEIEKVVRFQEEVGLDVLVHGEPERNDMVQYFGEQLKGFAFTTNGWVQSYGSRYVRPPIIVGDVSRPAPMSVKESVYAQSITTKAMKGMLTGPVTILRWSFPRVDIPQKFQALQLGLALRDEVNDLEAAGIKVIQVDEPAIREGLPLRAGEERQDYYQWAAESFRIATSGVKNVTQIHSHFCYSDLDPNHIKALDADVVSIEFSKKDDPNYIREFSDYPNHAGLGLFDIHSPRIPSKEEFISKIQNILEVYPVSKFWVNPDCGLKTRGWEETKASLTNMVEAAKYFREKYAKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUA2</accession>
    <name>A0A1E3NUA2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_81770</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat PAAF1/RPN14 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454216">
        <property type="protein sequence ID" value="ODQ56708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035915.1">
        <property type="nucleotide sequence ID" value="XM_019185292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUA2"/>
    <dbReference type="STRING" id="683960.A0A1E3NUA2"/>
    <dbReference type="GeneID" id="30202538"/>
    <dbReference type="OrthoDB" id="10257301at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051179">
        <property type="entry name" value="WD_repeat_multifunction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857:SF19">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT RPN14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="135"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="177"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ56708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="399" mass="44452" checksum="41525434FAED151D" modified="2017-01-18" version="1">MYTLSIQSSFEEVINDVVENRLQSDQFWINFVDAEDVQDGTMKEEIVTVFKDHQNIMFNNDNGLKFHIQKEHMYKVEYEGNIFNILSPSMTYKSGLKNTTTFDISPNKELLLIGNRDGDLRIVRTLDGGIERTIEEAHFAEVTKSLFFPSGQVGLTASLDFSIKIWDIASGANPRTFLSHKERITDLAMIGKGRNFVSSSLDGFIKLWDCASGTNFNSFKRKDSFKDGVTSLSITTVNSSKSPKNSPKNAYEFDTDGKRLLAGYVSGVICAYDLASKDQIFTTPSFGSEVVGIWSNDNTILSGHKDGYIANWDIRDVSKPLTKFKSVANPDLMAFSEEFTLISHPVSALCGISYRNATFNSPTPTILSGVCDEPVVGLKVMANERYVGSENGDIHLYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUA4</accession>
    <name>A0A1E3NUA4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_81614</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZC3H14 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035988.1">
        <property type="nucleotide sequence ID" value="XM_019185272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUA4"/>
    <dbReference type="STRING" id="683960.A0A1E3NUA4"/>
    <dbReference type="GeneID" id="30202518"/>
    <dbReference type="OrthoDB" id="438553at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900152">
        <property type="term" value="P:negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045945">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160091">
        <property type="term" value="P:spliceosome-depend formation of circular RNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.40:FF:000003">
        <property type="entry name" value="Nuclear polyadenylated RNA-binding protein NAB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.40">
        <property type="entry name" value="Nuclear abundant poly(A) RNA-bind protein 2, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040366">
        <property type="entry name" value="Nab2/ZC3H14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043094">
        <property type="entry name" value="Nab2/ZC3H14_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021083">
        <property type="entry name" value="Nab2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049017">
        <property type="entry name" value="Nab2_Znf4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048410">
        <property type="entry name" value="Znf-CCCH_2-like_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14738">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14738:SF29">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11517">
        <property type="entry name" value="Nab2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21803">
        <property type="entry name" value="Nab2-zf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21457">
        <property type="entry name" value="zf-CCCH_2-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="10">
        <location>
            <begin position="165"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="165"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="97"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ56781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="410" mass="45804" checksum="94309126091938C8" modified="2017-01-18" version="1">MSALDKNFKEELKNYLVAQISQIEGLADQATELSEYTVLLISNGRPFSETIEELQLIVDSDAVPQIVRTAYEALEKHLNPQDPKAPAQVTQDTNVDDDIIPETTDEDSKTSGIPTKPALGRPSGPRNGVTKTYGNGYVRSSFALKNADNLQKAMELSGLPIGARLNKKGRCHKFPHCPFQKDCKYAHPTRPCFQFPNCPNPPGTCNFLHPGEDDALIAELEKTKNDFLQQRQQNQIIKSQTGISLCKFGNMCTNLQCPFGHPTPANEDAKVIQLEWCPQNLKCEDSECQKAHSSLSKIKEVKPQQEKSLEACKFGMNCTNRYCKFRHARSAVICRDGENCTRIDCLFSHPINENCRFDTSCKNPNCLFKHPNGKSEAAPPATSMKWTKTDERVFAVPEDQVLEQAPPQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUF4</accession>
    <name>A0A1E3NUF4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATPase expression protein 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_65713</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for translation of the mitochondrial OLI1 transcript coding for the mitochondrial ATP synthase subunit 9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Binds to the 5'UTR of the OLI1 mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AEP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036038.1">
        <property type="nucleotide sequence ID" value="XM_019183971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUF4"/>
    <dbReference type="STRING" id="683960.A0A1E3NUF4"/>
    <dbReference type="GeneID" id="30201217"/>
    <dbReference type="OrthoDB" id="3980946at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024319">
        <property type="entry name" value="ATPase_expression_mit"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12921">
        <property type="entry name" value="ATP13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ56831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="534" mass="62073" checksum="9E5828EF963C58CC" modified="2017-01-18" version="1">MSLISRSKPLRLLAKRNISMVMNPEVQASNNTQDQVTQHPKDINASKKLTVAQFVNQTATRIPSFTKIQNRTRTENLITLTQEQIDHGSDIRPYLQQKNYKKLLKIAETMTLLRQYDSLTKGLTREEVSYFLKILVDGQLKEIENHNQVKFVASTPEYKTMIQKSKENAYIISFLYLNLIKEGFKFTPHEYELFLTLESKNYRTQQAIELIHHMETNQIPSSLKFWNLKLSIVGDADPGNWNVTSPYYTLSKRGNHGGFERKTQFGNLLQDFSQQIGDMVPNLESHKLILLGSGKHNLLDLARHHINDFWGISPQGKSSELNAKVEPNSPLYPDIELLEALIATFGVNNQLFEAFHYITEFQRYYNMDLSHAHHFWKLFINWTEMTNSEEPEVAAEIFDSVWSLMESNNVTFNTVLYQKRARHLVALNKLDDLVSDMAIVQDQVKKAYAGLKLKREEKLLSILYQGCCTVVFHTNVEEEFDLKQLGERIALDEGHLKDLQDLYKKELEVAEARRDRFVQLQKEYDEEDDENSLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUI6</accession>
    <name>A0A1E3NUI6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit L</fullName>
            <shortName evidence="4">eIF3l</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_81578</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit L family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035950.1">
        <property type="nucleotide sequence ID" value="XM_019185262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUI6"/>
    <dbReference type="STRING" id="683960.A0A1E3NUI6"/>
    <dbReference type="GeneID" id="30202508"/>
    <dbReference type="OrthoDB" id="15082at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03011">
        <property type="entry name" value="eIF3l"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019382">
        <property type="entry name" value="eIF3l"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13242">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13242:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10255">
        <property type="entry name" value="Paf67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="509"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="523"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ56743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="538" mass="62829" checksum="0392F504BB9E92FC" modified="2017-01-18" version="1">MADSKNTAIPEPVEQFIKNFYKAYLENNVYELHSCYENGFNKLTESYYKNTPWPDPVEQVAPLVNDDQIFLVLYSEVYYRHVYARLNPTLEHRTGSYNNYCNLFNLLLNSEDGPVTFDLPHKWLWEIVDEFIYQFNQFSVYRTKLILNKKNADPVELSYVQEHHEVWSAYSVLNALYSLIGRSQIVEQLKDSKSVLYTNRVNTEIAGEYGSLPLYRNLGYFSVIGLLRIHTLLGDFTLALKTMEHIDLDKRAFFTKIPGCHFTTYYYVGFCYLMLHRYSDAIKAFSHILLYISRTKNISRSGQYDIVTKKSEQMYALLAILVGLAPTRLDDTLSSGIKEKFGDKFLKIQRGGEESLSVYEDLFKFGSPKFISTSIPIPDHVNDPLYLHLKIFMLKVKNIVFIPTLKSYLSLYSKLDINKLAHFLEMDENELMSTLLTYKLNNRQLKWIDGELLNGEFVNVYDLDISLENDDGKTFIHVKETKNIRKFADWFIRNTIKNYSAQEVISNASKESFKESQQNNKNTKNHHHHHQDHKTSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUT0</accession>
    <name>A0A1E3NUT0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_86273</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP12 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036050.1">
        <property type="nucleotide sequence ID" value="XM_019186029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUT0"/>
    <dbReference type="STRING" id="683960.A0A1E3NUT0"/>
    <dbReference type="GeneID" id="30203275"/>
    <dbReference type="OrthoDB" id="2192888at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012978">
        <property type="entry name" value="HEAT_RRP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057860">
        <property type="entry name" value="HEAT_RRP12_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052087">
        <property type="entry name" value="RRP12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48287">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48287:SF1">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25772">
        <property type="entry name" value="HEAT_RRP12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08161">
        <property type="entry name" value="RRP12_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRP12 N-terminal HEAT" evidence="6">
        <location>
            <begin position="27"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="RRP12 HEAT" evidence="5">
        <location>
            <begin position="352"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1006"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1075"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1131"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1033"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1093"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1136"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1151"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1161"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1179"/>
            <end position="1193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ56843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1193" mass="133105" checksum="6E64D49E7FB3FEE0" modified="2017-01-18" version="1">MSFEGDKGLEELDFEFKLQKIRNQITSKLENQKHLALILSAVEENINDQNNSKSPVAYFVSFLSLLDQAIDSESDEILDSALAISSAYFLDLTLPFIPKTLLKSKFPEILTKLAPAITNSNAEAALIRSAIGALETLLIAQDLNSWNNTSLNINPRRGFGGLLEYSLDPRPKVRKRAQEAIHKILANPPPGPALEHVASGMAADFAIKSIVTLLSTKPNQKTNKEHSAQLIHALQLISSITSANTWPAAKIENLCDLLLEISKTNDQYLVSAAFGGFEGLFNSMTNEIDNDKFVKVLNIIFDLKPSINDSHLAAPWIAVVSKGVTAYSKIEPLEAVKKIPQVLEILKAFFASEVSDIYISASQSIIAIISEGIPEKFLLLPNGDDITPEIYEEVDDVITRISEIITELLSVKYTSATKEILEVIATCFTKLRMRTNPDFLKPLEIVGEWRTNEDFEHKAEAEAVIGNAITELGPEVVLNVLPLNLLDANKTGRAWLLPLLRDNVRFARLGYFIQHFMPLIEAFSEKVGSLDKNSVHSKIFQTIIDQTWSLLPHFADLPNDLQKSFTDEFAQTLSQLLYSNVELRVNVCHALRLLVESNVAYANGALADDELLSQQFSIKEAQSNVEYLATKSSNILAVLFNVFSQTVPESRGFVMETIDAYLSISSPTDLEATFNKVSSLLKDALESEANQQQAKNKSKLSVTMMDLVVAMAKYVPESSYYALFMIFNQVVTSEEPLLQKRAYRIISKLVETPTGTQALSKFIESIEKVIIDNTNNVHPSAKAARLSAISTILEILPSDHLNFIPGVLSEITLSTTSVNEKTRELSYKTLIEMGNVMNKGGVVKLSKLDGFPDDTPDSEGSLEEYLKMVLGGLGGDNNHQISITITAISCLIYEFKDQISTDLLVEITSTVDLFLTTNNKEIAKSAIGFAKIAAISLPEELVRPKLKTMLENLLRWSHEHTGHFKSKVKHIIERLIRRFGFEAIEENFPEEDKKLLNNIRKTKARAKRRQAEEGEEDAEDSKKSKFSNAYEEVLYESEDEDEDDAEEGNNKKGNQYILQSNDEPLDLLDRQALSHISSSKPQKKGGRKINASEFEKDGRLHFNDKNNADDEDPLKSITSGINAYVEAVRSGPVKGQKNKFKFKRGAKAYNDEDDGEDDGEETSRPARNERPKFVNKNNKVGKPRQKFKARKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV84</accession>
    <name>A0A1E3NV84_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_81267</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036243.1">
        <property type="nucleotide sequence ID" value="XM_019185204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV84"/>
    <dbReference type="STRING" id="683960.A0A1E3NV84"/>
    <dbReference type="GeneID" id="30202450"/>
    <dbReference type="OrthoDB" id="4095993at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="419" mass="47859" checksum="2BF5396876C1618B" modified="2017-01-18" version="1">MKEGSNQPSDQRPDEQTSSNNSSSNADKDAANENQSNGHDQDNNNTNNNNNGQLNSNINNYIENLDVANVLAVDLSNLENQQGYLDPSIAAAYRRAALEAQRARAEADSQNANEEGSEEHQEYIPGIPQLPPGSDPKKTCPFCQRTFSHAGSMGRHLDLKKGTESHPVDLITRMRADVKRRGDIEVIKQRRRLRSRKYYAREDVKERSRAKRRVRERILRAKSVAEREFLSRFNKPELEPHPTFARLVIFFLSPNQWPHDPPTLETYRLLCGFLNSKFLDGKQGEEIHKKIMEQVHNASENWLKLSDTSKQETWASELRKAAMESLKNISLFDLSSRDEWIMQQARHRALHDEHQGDTAESGNDGEGRDDDEDDEKTRIHKNADDNDNNLGSENYYNQEELSAVAEAVVNNRNNNEDQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVH8</accession>
    <name>A0A1E3NVH8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I, II, and III subunit RPABC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_81328</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036312.1">
        <property type="nucleotide sequence ID" value="XM_019185215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVH8"/>
    <dbReference type="STRING" id="683960.A0A1E3NVH8"/>
    <dbReference type="GeneID" id="30202461"/>
    <dbReference type="OrthoDB" id="248779at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1340.10:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.940.20:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1340.10">
        <property type="entry name" value="RNA polymerase, Rpb5, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.20">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00025">
        <property type="entry name" value="RNApol_Rpo5_RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014381">
        <property type="entry name" value="Arch_Rpo5/euc_Rpb5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005571">
        <property type="entry name" value="RNA_pol_Rpb5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036710">
        <property type="entry name" value="RNA_pol_Rpb5_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000783">
        <property type="entry name" value="RNA_pol_subH/Rpb5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020608">
        <property type="entry name" value="RNA_pol_subH/Rpb5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035913">
        <property type="entry name" value="RPB5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007129">
        <property type="entry name" value="PRK09570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01191">
        <property type="entry name" value="RNA_pol_Rpb5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03871">
        <property type="entry name" value="RNA_pol_Rpb5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000747">
        <property type="entry name" value="RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53036">
        <property type="entry name" value="Eukaryotic RPB5 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55287">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01110">
        <property type="entry name" value="RNA_POL_H_23KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb5 N-terminal" evidence="8">
        <location>
            <begin position="7"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase subunit H/Rpb5 C-terminal" evidence="7">
        <location>
            <begin position="142"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="215" mass="25024" checksum="0750D17CC6A799BC" modified="2017-01-18" version="1">MDQENDRAVSRLWRAFRTIKEMVKDRGYFITQEEIDMSLEEFRSKNCDAMGRPQRKLMSFQANPTAESLEKFPNMGSLWVEFCDEASVGIKTMKNFVIHISEKNFSTGIFIYQNGITSSANKLIPTVAPAVIETFQENDLIVNITHHELVPKHICLSDDEKKELLERYRLKESQLPRIQREDPVARYLGLRRGQVVKIIRRSETSGRYASYRICL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVM2</accession>
    <name>A0A1E3NVM2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_35844</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036362.1">
        <property type="nucleotide sequence ID" value="XM_019182362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVM2"/>
    <dbReference type="STRING" id="683960.A0A1E3NVM2"/>
    <dbReference type="GeneID" id="30199608"/>
    <dbReference type="OrthoDB" id="3978754at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19079">
        <property type="entry name" value="AKR_EcYajO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000004">
        <property type="entry name" value="Oxidoreductase, aldo/keto reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050523">
        <property type="entry name" value="AKR_Detox_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364:SF15">
        <property type="entry name" value="ARYL-ALCOHOL DEHYDROGENASE AAD16-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364">
        <property type="entry name" value="NADH-SPECIFIC METHYLGLYOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="24"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="361" mass="40805" checksum="3FB753EC6E4882E6" modified="2017-01-18" version="1">MPNETSQLVPWKNLGKSGLKISNIIIGCMSFGSKNWEEWLIEDKEEIFKILKTGYDHGIRTYDTADVYSNGLSEKLLGEFLKKYQIKRDKVVILTKVFYPVDDDYPVGYPGNVLDNIGSEVDKLNFQNNRGLSRKHILDAAKNSVERLGTYIDVYQIHRYDPSTPQEETMKALNDCIEAGYTRYIGASSMKAVEFAEYQHIADKNGWQKFISMQSFYNLLNREDEREMNYYCDKTGVGLIPWSPLAGGTLARPVPKEESEFTTRTQVTKFNALIGGGFTAEDNTKIVDIEIIKRVGEIAGKKNVTMAAVATAWVISKGAAPIIGFSSEKRVLDAVIGANLKLTEDEIKYLEEPYQPRLRAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW34</accession>
    <name>A0A1E3NW34_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD-dependent protein deacylase</fullName>
            <ecNumber evidence="5">2.3.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Regulatory protein SIR2 homolog 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_86108</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(6)-glutaryl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-glutaryl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11875"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87828"/>
            <dbReference type="ChEBI" id="CHEBI:87829"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(6)-malonyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-malonyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11878"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87831"/>
            <dbReference type="ChEBI" id="CHEBI:87833"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(6)-succinyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-succinyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11877"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87830"/>
            <dbReference type="ChEBI" id="CHEBI:87832"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="5">In contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-75 and Arg-78) that bind to malonylated and succinylated substrates and define the specificity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the sirtuin family. Class III subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="5"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036417.1">
        <property type="nucleotide sequence ID" value="XM_019185997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW34"/>
    <dbReference type="STRING" id="683960.A0A1E3NW34"/>
    <dbReference type="GeneID" id="30203243"/>
    <dbReference type="OrthoDB" id="424302at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061697">
        <property type="term" value="F:protein-glutaryllysine deglutarylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036054">
        <property type="term" value="F:protein-malonyllysine demalonylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036055">
        <property type="term" value="F:protein-succinyllysine desuccinylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01412">
        <property type="entry name" value="SIRT5_Af1_CobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01121">
        <property type="entry name" value="Sirtuin_ClassIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027546">
        <property type="entry name" value="Sirtuin_class_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF10">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="8">
        <location>
            <begin position="6"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="154"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5 6">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="110"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="266"/>
            <end position="268"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="336" mass="37438" checksum="17D3255995C3E2A5" modified="2017-01-18" version="1">MDNGVNKAESSDLESFHDYLLQANNILAVVGAGLSASSGLSTFRGAGGIWRNFSPIDLATPDAFHFDPGLVWQFYSARRYNALKAKPNRGHYALAELSKRRNGKFLTLTQNVDGLSTRAGHDKDTLLELHGSLFTLKCTNFFCNHIDQDNRQHPLTPALEGSEDEFPKKRKLNDQNDKEESKKRARTDDALERTKSHTSIASTGSSPDIKPVQSIPEKDLPHCPECKEGLLRPGVVWFGESLPLKAVDTADEFLINNKIDLVLVIGTSGTVWPAMGYVERVQKRGGKVAVFNTEIDMEQIKKDKNWGFIGDASKWLPIALEPIIGSDFVPRDYKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWE2</accession>
    <name>A0A1E3NWE2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Autophagy protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_80825</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in cytoplasm to vacuole transport (Cvt) and autophagic vesicle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Conjugated with ATG12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Preautophagosomal structure membrane</location>
            <topology evidence="1 6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ATG5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036698.1">
        <property type="nucleotide sequence ID" value="XM_019185132.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3NWE2"/>
    <dbReference type="GeneID" id="30202378"/>
    <dbReference type="OrthoDB" id="272162at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034274">
        <property type="term" value="C:Atg12-Atg5-Atg16 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044233">
        <property type="term" value="C:mitochondria-associated endoplasmic reticulum membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061908">
        <property type="term" value="C:phagophore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019776">
        <property type="term" value="F:Atg8-family ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.190">
        <property type="entry name" value="Autophagy protein Apg5, helix rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007239">
        <property type="entry name" value="Atg5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048940">
        <property type="entry name" value="ATG5_HBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042526">
        <property type="entry name" value="Atg5_HR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048939">
        <property type="entry name" value="ATG5_UblA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042527">
        <property type="entry name" value="Atg5_UblA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048318">
        <property type="entry name" value="ATG5_UblB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13040">
        <property type="entry name" value="AUTOPHAGY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13040:SF2">
        <property type="entry name" value="AUTOPHAGY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20637">
        <property type="entry name" value="ATG5_HBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20638">
        <property type="entry name" value="ATG5_UblA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04106">
        <property type="entry name" value="ATG5_UblB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3 6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="4 6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Autophagy protein ATG5 UblA" evidence="9">
        <location>
            <begin position="8"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy protein ATG5 alpha-helical bundle region" evidence="8">
        <location>
            <begin position="125"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy protein ATG5 UblB" evidence="7">
        <location>
            <begin position="189"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="277" mass="32088" checksum="B84A47410EC7BA29" modified="2017-01-18" version="1">MSELSSKIWNGSLSVCVVLSPSDSNQMTHNREFYLTVDRNSYFPLYYTKLLRFFEPYLLVPGWSKSSEWWLEFEEVPLKWNYPIGLLYDTLTSLDPQKRKADSTWRLILHYRNYPDYILPLPDYKTLESHWVNQVKESCYVQHGTAKLVMVLSKQDSSDLWKSVEDHDVQKFWSIMPKITSKSSKKLKNIPLRLYLPVSDKVIDVPIEAYQSDGDPTKLGDALHKVVPDLFPSTKNNIIAKPVLHGITLPLNIPLTELSYECIYVDGFLHVSIVLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWF0</accession>
    <name>A0A1E3NWF0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_36937</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="82"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="226"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <end position="276"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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            <end position="330"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="342"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="386"/>
            <end position="410"/>
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    </feature>
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        <location>
            <begin position="32"/>
            <end position="440"/>
        </location>
    </feature>
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        <location>
            <begin position="450"/>
            <end position="487"/>
        </location>
    </feature>
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        <location>
            <begin position="456"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="469"/>
            <end position="479"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="ODQ56897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="487" mass="52274" checksum="F428C3608D37B74F" modified="2017-01-18" version="1">MSDFTGEPTGTGTGGDSLTTDMNKQFDLANMAWIGVAGVLVWIMIPGIGLLYSGLSRKKHALSLLWASMMAVSLVSFQWFFWGYSLVFSHNVYGQFLGSLQFIGLRNVLGAPSVVSSVPDILFCFFQGMFACVTGCLMIGGACERARLGPMMVFLFIWITVVYCPIACWTWNPEGWLAKLGALDYAGGGPVHISSGHGALIYALILGKRNDPVAGKGLPKYKPHSVTSVVLGTVFLWFGWFGFNAGSAGNATIRAWYSAVSTNLCAAVAALTWMFVDYFRSGGKWTTVGLCSGAIAGLVGITPAAGFVPVWAAIPIGVCVGVGCNFAVDLKNLLRIDDGLDVYALHGVGGAIGSVLTGIFAADYVTATDGATVIDGGWINHNYKQVGYQLAAICATIAYTCTCTSIILLVQDRIPFLRLRLRPEEEELGTDNVQIGEFTYQEEEIYIPEPVRSHTSQREAPKHNLIDDQINTDGTNSTGEVEKKSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWI1</accession>
    <name>A0A1E3NWI1_WICAA</name>
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        <recommendedName>
            <fullName evidence="11">Urea active transporter</fullName>
        </recommendedName>
    </protein>
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        <name evidence="9" type="ORF">WICANDRAFT_35162</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
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    <dbReference type="OrthoDB" id="6132759at2759"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="SLC5sbd_DUR3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUR"/>
    </dbReference>
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        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154:SF3">
        <property type="entry name" value="DUR32P"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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            <begin position="16"/>
            <end position="37"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="58"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="92"/>
            <end position="111"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="137"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="187"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="297"/>
            <end position="322"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="374"/>
            <end position="394"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="406"/>
            <end position="428"/>
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    </feature>
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        <location>
            <begin position="463"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="502"/>
            <end position="522"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="594"/>
            <end position="611"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="631"/>
            <end position="650"/>
        </location>
    </feature>
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        <location>
            <begin position="543"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="667" mass="72466" checksum="C1EDBFCDCBA161C0" modified="2017-01-18" version="1">MSAIQSLDLLTQGAGYGVLIGVGAAFALFIVVATRLTSKHLGEDAQSTEMFMVANRSVGVGLTASCVYSSWTWATELLWCVTMVYLYGVQSAFWYGAGLAVQICIMSVLGIEAKKKIPTSHTCLEIVQLRYGKIAHLLYMFLCLVNNLVSASSMILGAAGAISAMCDNLHIVASTLLIPFGVLLYTAVGGLKSTFLTDYVHSLILLIILCYICTAAVTSEHIGGIGGLYDKLVEKAAAGEIKYIEGNYKGSYITGKSQGAIFFGLIHSIGDTGLTVMDSSFWQKSFSANIKASVPGYLIAGALIYANVWALGMVIGLGNVVLEDNQIFPTYPNKMSTFEVNSGFGLIYTVKALLGTKALGALLLVLYLSVTSTVSAQLISVSSIISFDIYRTYFKPNASNKQLITVSHLGVVFFGLFSAGFSIMLHYVGVDMTWFGYFYSMLNTPGVIPMILLVVWKDQSKAAFIISPILGIIGGLAVWTGSAYALYGSVTIKTTGEQLPCLYGGLTALFLPGVATVLISLIKPANYDWNNMRDQSNLIFVKNDDDSDTQPASTNDLVTEKGQNADVKEESKLTSSEIESVDVNEDNLIKKYRYLSYSATIIVLLVTWVIWPLPLYRDYIWSKAFFTGNVVVGFIWLYLALVMIGLYPLYDGRHSLKRVFNGVLGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWK7</accession>
    <name>A0A1E3NWK7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP synthase subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_64937</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Subunit b, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. In yeast, the dimeric form of ATP synthase consists of 17 polypeptides: alpha, beta, gamma, delta, epsilon, 4 (B), 5 (OSCP), 6 (A), 8, 9 (C), d, E (Tim11), f, g, h, i/j and k.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the eukaryotic ATPase B chain family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036796.1">
        <property type="nucleotide sequence ID" value="XM_019183834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWK7"/>
    <dbReference type="STRING" id="683960.A0A1E3NWK7"/>
    <dbReference type="GeneID" id="30201080"/>
    <dbReference type="OrthoDB" id="67388at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2210:FF:000002">
        <property type="entry name" value="ATP synthase subunit 4 mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008688">
        <property type="entry name" value="ATP_synth_Bsub_B/MI25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013837">
        <property type="entry name" value="ATP_synth_F0_suB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12733:SF3">
        <property type="entry name" value="ATP SYNTHASE F(0) COMPLEX SUBUNIT B1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12733">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE B CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05405">
        <property type="entry name" value="Mt_ATP-synt_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161060">
        <property type="entry name" value="ATP synthase B chain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0138">CF(0)</keyword>
    <keyword evidence="4 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="233" mass="25584" checksum="D143AF251C970726" modified="2017-01-18" version="1">MSLRQLSLRAIRPASFALRSAPIGVRCLSTQTPEPKAKATSIIDALPGNSVLSKTGILATGTAASIYAISNELYVVNEESILLGCFLAFSGLVVKYVAPLYNDWAESRIKSVSEILNSSRVKHVEAVKDRIESVSELKDVVNTTKVLFEVSKETVELEAQAFELKQKVELASEAKSVLDSWVRYEASVRQLQQQQLAQTVIANVEKELTNPKFQDKVLQEAVADIEKVFASAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWX0</accession>
    <name>A0A1E3NWX0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_65007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036868.1">
        <property type="nucleotide sequence ID" value="XM_019183845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWX0"/>
    <dbReference type="GeneID" id="30201091"/>
    <dbReference type="OrthoDB" id="3980325at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="105" mass="11772" checksum="2F2EC1531DFF0F1F" modified="2017-01-18" version="1">MNLKGPKPMSGRQRAGMTGGILGLALSAYILGSNYEIVKFTPPETNPDGSVKAKKFNIRFAEMQPLKLKPEVAAKQEQVLKEKEERDKLMKDIQEREQIVQEPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWY0</accession>
    <name>A0A1E3NWY0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_97027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036841.1">
        <property type="nucleotide sequence ID" value="XM_019186553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWY0"/>
    <dbReference type="GeneID" id="30203799"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="147" mass="17009" checksum="F6D23800BE38BD7A" modified="2017-01-18" version="1" fragment="single">MADIKSIHPITTDIPKSFVQKKTEDMERYISEVIKNIPDRIVIRCISESLKNIKDGYKIGRQTNKAAIEDLLRRQIIAPITEFFEMIRDYDTTSNKNALECCGHFSFARISKPERSSDIRLRSNVELITPDFSFINTTPSTYRGYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX04</accession>
    <name>A0A1E3NX04_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF155 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_85802</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036908.1">
        <property type="nucleotide sequence ID" value="XM_019185950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX04"/>
    <dbReference type="GeneID" id="30203196"/>
    <dbReference type="OrthoDB" id="18302at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF4">
        <property type="entry name" value="SPORULATION PROTEIN RMD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="511"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="DUF155" evidence="4">
        <location>
            <begin position="304"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="126"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="544" mass="62588" checksum="1FCBF4CE866B8A30" modified="2017-01-18" version="1">MAQRPTAKRSPSILVTDSRNANHKSAPFAGAAHNSSNNGSNHLSKTARLKKRNSGKFSDISVSNILSDVSDIPSGRREERLSSGSINLGSSLRDQRRKLYMKPFQPLPSRTSKTSQKLVLIPDEDEEYDHEIGGPRPSSKSARSKAEKMSKEKRGKKLSRVTAYLVSDGFNLKRTAQFLKETHDIYPRIYDEALYCPYCLPLLPGRDGFRIKSNKSAKNASGVAYMERLIDTSERRDHHYEYYSGVETPEDANNNYEIDKPETEEANYGPFDPSEPQFFAENVQENSAASNAKSQEMIKHHAEMFIFNYGVVVFWNFTEIQEKNILGDISFARNENDNKQLLIRPIDEHDIEKEQFHFEYDMDTERPRIYNDMITLRSGDHLIKLTMSHAIAQSTKLCRFESRITPILYSVSKLPKRLALTGKLGLKREQLIKKSGKLFKLRVDVNLSSNVLDTPEFFWTFEPSLHPLYNAVKEYLEIDQRVEVLNDRCKVFLEFIDIVADSIAEKNMTRITYMIITVFFLSLVVSCLEIFVRYMIIDRNKHFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXK1</accession>
    <name>A0A1E3NXK1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="5">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_12004</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037080.1">
        <property type="nucleotide sequence ID" value="XM_019180514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXK1"/>
    <dbReference type="STRING" id="683960.A0A1E3NXK1"/>
    <dbReference type="ESTHER" id="hanan-h6uwn6">
        <property type="family name" value="Fungal_carboxylesterase_lipase"/>
    </dbReference>
    <dbReference type="GeneID" id="30197760"/>
    <dbReference type="OrthoDB" id="6846267at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009027328" description="Carboxylic ester hydrolase" evidence="5">
        <location>
            <begin position="18"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="6">
        <location>
            <begin position="26"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="559" mass="61760" checksum="6B08645699A45D62" modified="2017-01-18" version="1" fragment="single">MLFNIIAITALIPIASTAPITATSSPPIVQIQNGSIQGDYLEKFNQDVFLGIPYAQPPLGSLRFKPPQSFNDSWNGTKIFNQYSDACIASGASDNKLLPQSENCLTVNVVKPHGVGNENLPVAIWIYGGGFQDGSSSRPAYNLSYIVQNGVEIGKPFIGVSFNYRLSGLGFLGGQEVVNKGFTNVGLRDQIQAIHWIHENIHQFGGDSDHLVIWGESAGAISVSKLLSSNQLGDYIKGAIIESGPALFPNITSGANEARQIDYNTLSTYFNCSEATDSLECLQYVDINELFHVFNVSNGVLQTGFRYPYIDGDIIPKSSYQILSDDQSLRVPLLIGTSTDEGSQFVPKNLNTTEELKEFLKGAFPSLTDSSINKLDELYPIDDPLVSTPLDPSSYSKLPISYPPTFGAQYPRLATLYGDLVFFGQSRIMSKFYSQKSPVYKYRFNIPDLETSNQSYYGAGHFQEVVYVFDNDQAPIDTSSGATWNPSPKSPAIAESISKIWVNFIADLNPNVDQTKIPIETPEWPEYKDGEQNMVFDLNGFYLEKDDKRAQQIEFIESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY59</accession>
    <name>A0A1E3NY59_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Abscisic acid G-protein coupled receptor-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_64289</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037348.1">
        <property type="nucleotide sequence ID" value="XM_019183702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY59"/>
    <dbReference type="GeneID" id="30200948"/>
    <dbReference type="OrthoDB" id="264392at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025969">
        <property type="entry name" value="ABA_GPCR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022535">
        <property type="entry name" value="Golgi_pH-regulator_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015672">
        <property type="entry name" value="GPHR/GTG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15948">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 89-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15948:SF0">
        <property type="entry name" value="GOLGI PH REGULATOR A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12430">
        <property type="entry name" value="ABA_GPCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12537">
        <property type="entry name" value="GPHR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Golgi pH regulator conserved" evidence="7">
        <location>
            <begin position="192"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Abscisic acid G-protein coupled receptor-like" evidence="6">
        <location>
            <begin position="345"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="560" mass="63888" checksum="2051E637D842018B" modified="2017-01-18" version="1">MSISVSLFFLVSALLVFYNTYTHLWFQIIKIFEPTKFNSKYADIKDDDLNSSSFVKLISSDELSISNQRIIDLLKKFSRIAFSTSITTSFLVIEFILYHFISDGGNQDNHTALNLWNLNLVVITINLIVIHPILITVLLVNKFFKSSLLNNLTIKSIATLIIFIFWCLTLSKFDSFTDLNIVDNTDSHVIAYYLLRISVIGITLIAALNGVGSFSTAYYNFFKRFKRSNSTSSFQQSTPQYLETLEKTLQNTSNMIKSKEIEYEKQQSSRPYADDSQLNKRPGFQPKKSFLDLNTLNQTLNPFKSNTSKVENELISEINSLKIIKNDIYLKMLKVERNINQSTSQDKFHVKIRKIIQLSLSIYCTFKILQVTSIKIISIFNSKFYDEVSTKESDPLVLTIVKTIQIFITVKDEEFITNQLSFIVSGALFLCSINGVFMTFQHLYRFLPLDLSKLSKTTTPNTKYQSVSIIKNLLISELTGIYTLATILILKSNLTSNFSSKLNTLFSLTNQNISIVQLDNWFDKIFLVSVVLTAVCIKTAEYLSDDIDGFEDQALAGKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYC1</accession>
    <name>A0A1E3NYC1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-terminal acetyltransferase A, auxiliary subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_16147</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037302.1">
        <property type="nucleotide sequence ID" value="XM_019180678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYC1"/>
    <dbReference type="STRING" id="683960.A0A1E3NYC1"/>
    <dbReference type="GeneID" id="30197924"/>
    <dbReference type="OrthoDB" id="10263032at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010698">
        <property type="term" value="F:acetyltransferase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1040:FF:000003">
        <property type="entry name" value="N-terminal acetyltransferase A, auxiliary subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021183">
        <property type="entry name" value="NatA_aux_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767:SF2">
        <property type="entry name" value="N(ALPHA)-ACETYLTRANSFERASE 15_16, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12569">
        <property type="entry name" value="NatA_aux_su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000422">
        <property type="entry name" value="N-terminal-AcTrfase-A_aux_su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="685" mass="79332" checksum="8ABFF97FDEF6ADBF" modified="2017-01-18" version="1" fragment="single">MSRRKGQPIITSKEDGSFREALKLYESKQYKKSLKLTEGILKKNSNYADAYALKALLLHFLNEPTDSESYVGKAIAKNPDSPIINHVLGILRRNQLNYVEAAKFFKKALDNGATNKQIWRDLSTMEVQNRDYKNLSISRHAYLEEFLGFRANWTGLATAHHLNKDYAAAEKVLTKFEELAKGKLTDAEMFEHSELQLYKNHLINEQDTERALKDLDELDTLDKLDEMESRAKYLMKLGRSKEASKVYRQLLKRNPDNFKYYTLLEASLGTLTKSPKYRDALYQKLVKFYPRSDAPRFIPLTFLKGELFAQRVGDYILSLLKRGVPATFTNVKPLYSDPEKVEIIEKIVLDFLPKSLEISPLVWVWTSYFLSQHYLKKGDVTSAKTYIEAAVEHTPTLVELYILKARVYKHLGDFNKAADIMNEGRLLDLQDRFINSKAVKYYLRSDNVDEALTLVSLFTKNDNSVNGVKDLHLMQANWFIIESAEAFFRLYETSVAKNELEDAQKYLGLSVKRFNGIVKIFEEYYNDQLDFHTFCLRKGTARAYIDMVYWEDKVFANPVYCRAVEGVSKIYFQLFDKPTKEEEHEDHEDHKKKDKKAKKDKIAKQKALEGERAKHLAYVDDSDVFGEKLIETSTPLEDFQKIFFNHLLEQGGDKILTNDLQYNVQFRLNKVALAMGALTKLKTLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYG4</accession>
    <name>A0A1E3NYG4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vacuolar protein sorting-associated protein 20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_24485</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037448.1">
        <property type="nucleotide sequence ID" value="XM_019181032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYG4"/>
    <dbReference type="STRING" id="683960.A0A1E3NYG4"/>
    <dbReference type="GeneID" id="30198278"/>
    <dbReference type="OrthoDB" id="441172at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF5">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="18455" checksum="C5ED235A0B6B4F4B" modified="2017-01-18" version="1" fragment="single">PKVTAQDKAILQVKLQKDKLLKYQKKSNLLLKSETEQIKTYLRQNDKRSAKVLLKRTKYQETLLENVSSQIMNLENMIQNIEFKLVEKEFLKGLTNGNEILKKLNKEMNINDVEKLMDDVNDNIQYQEEIDQVLSTSIVGKDVEDEIDEELRQLEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYV3</accession>
    <name>A0A1E3NYV3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Dipeptidyl peptidase 3</fullName>
            <ecNumber evidence="4 15">3.4.14.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 15">Dipeptidyl aminopeptidase III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14 15">Dipeptidyl peptidase III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_80457</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Release of an N-terminal dipeptide from a peptide comprising four or more residues, with broad specificity. Also acts on dipeptidyl 2-naphthylamides.</text>
            <dbReference type="EC" id="3.4.14.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 17">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15 17">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the peptidase M49 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.4" evidence="4 15"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037520.1">
        <property type="nucleotide sequence ID" value="XM_019185067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYV3"/>
    <dbReference type="STRING" id="683960.A0A1E3NYV3"/>
    <dbReference type="GeneID" id="30202313"/>
    <dbReference type="OrthoDB" id="4694525at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.30:FF:000001">
        <property type="entry name" value="Dipeptidyl peptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.30:FF:000002">
        <property type="entry name" value="Dipeptidyl peptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.30">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005317">
        <property type="entry name" value="Dipeptidyl-peptase3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039461">
        <property type="entry name" value="Peptidase_M49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422:SF11">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE III-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03571">
        <property type="entry name" value="Peptidase_M49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007828">
        <property type="entry name" value="Dipeptidyl-peptidase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 15 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8 15" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15 17" id="KW-0862">Zinc</keyword>
    <feature type="active site" evidence="16">
        <location>
            <position position="433"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="432"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="490"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR007828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR007828-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR007828-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ58313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="680" mass="77163" checksum="52AF6DFC12508294" modified="2017-01-18" version="1">MSSTNIDISNFHADAKAPIVSLTAKSFFDQLTDKEQKYAHFFSKAGHWGSRIVLRQVSHESEPIFDLILQIHKNLKSDYSSLKIDEDVTKGYLEYASQFLSNLGNYKSFGDKKFIPRISVEDFEKIVKLSNSDLDLFNKIKEPLYSIDEKSALLGFPSDGHVSGYYFGDVSKKNIDLIKKVLADHKVLYENLRVKKISETEFIILVASELTNNKTSYPNEIEADGIKLTLKFGDHSKEFTKIIENFKEAKDVVANETQSKMIDAYVESFETGSIDAHKESQKYWVKDISPVIETNVGFIETYREPGGVAGEWEALVSIQNKERTLKFQNLVNSAENFIKLLPWTKEYEKDKFQAPDFTSLEVLTFAGSGIPAGINIPNYDDIRLNIGFKNVSLGNILNAKSGKEPITFIHDDDYELFNKYRNDSFEVQVGIHELLGHGSGKLLSELHDGSFNYDHSNPPIGIDGKPVTTFYKKGETWGSKFGSLAGAYEECRAEVIALYLITNEELLKIFGFDSKEDQDAIIYSGFLQMSRAGLLALEFWDPSSQKWGQPHMQARYSILKTLLNAGLVELVYTKEDYSDLYVKLDKSKIETIGHEAIKDYLRHLHIYKASGDVENGSNYFIDRSSVTPELAKFRPAVLKARLPRKQFIQANTEIIDGKVVLKEYEESEVGMIQSFVEREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ91</accession>
    <name>A0A1E3NZ91_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Orotidine 5'-phosphate decarboxylase</fullName>
            <ecNumber evidence="3 10">4.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_64643</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>orotidine 5'-phosphate + H(+) = UMP + CO2</text>
            <dbReference type="Rhea" id="RHEA:11596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="4.1.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the OMP decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.23" evidence="3 10"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037716.1">
        <property type="nucleotide sequence ID" value="XM_019183779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ91"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ91"/>
    <dbReference type="GeneID" id="30201025"/>
    <dbReference type="OrthoDB" id="10263753at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04725">
        <property type="entry name" value="OMP_decarboxylase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000114">
        <property type="entry name" value="Decarboxylase,orotidine phosphate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018089">
        <property type="entry name" value="OMPdecase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01740">
        <property type="entry name" value="pyrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278:SF9">
        <property type="entry name" value="URIDINE 5'-MONOPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00156">
        <property type="entry name" value="OMPDECASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 10" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orotidine 5'-phosphate decarboxylase" evidence="11">
        <location>
            <begin position="32"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="8">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="8">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="8">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR614732-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR614732-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ58509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="267" mass="29224" checksum="A0F636B76D8355FB" modified="2017-01-18" version="1">MVQTLTYPQRAEAHPSPLAKRLFGVMESKKSNLCASIDVRTTKEFLELVDTLGPYICLVKTHIDIIDDFSIEGTIEPLKKLASKHNFLIFEDRKFADIGNTVKAQYSGGAFKIATWSDITNAHGVTGEGIVRGLKEAASEVSQEPRGLLMLAELSSKGSLAKGEYTKQTVDIAKTDKDFVIGFIAQNDMGGRDEGFDWLIMTPGVGLDDKGDGLGQQYRSVSEVVAGGSDIIIVGRGLFGKERDPKVEGERYRSAGWNAYLKRTSQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZB9</accession>
    <name>A0A1E3NZB9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_33891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037605.1">
        <property type="nucleotide sequence ID" value="XM_019182124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZB9"/>
    <dbReference type="STRING" id="683960.A0A1E3NZB9"/>
    <dbReference type="GeneID" id="30199370"/>
    <dbReference type="OrthoDB" id="2157498at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029164">
        <property type="entry name" value="PIG-Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39400:SF1">
        <property type="entry name" value="PIG-P DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39400">
        <property type="entry name" value="YALI0E29227P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15159">
        <property type="entry name" value="PIG-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="109" mass="12253" checksum="FFAB38EE475EBF9E" modified="2017-01-18" version="1">MTSTVDEAEVPLPLIKPRSLKHKLRSGNPEAEVRNGYILLISTWFIFIVSTGSLFELWSIPNPQNSDFPISNYYTHAILLIPVAAWLWCVISWTGLKLFKHAKGGAIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZC2</accession>
    <name>A0A1E3NZC2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RAVE complex protein Rav1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_101695</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037720.1">
        <property type="nucleotide sequence ID" value="XM_019180330.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3NZC2"/>
    <dbReference type="GeneID" id="30197576"/>
    <dbReference type="OrthoDB" id="342131at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043291">
        <property type="term" value="C:RAVE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045022">
        <property type="term" value="P:early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043254">
        <property type="term" value="P:regulation of protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052208">
        <property type="entry name" value="DmX-like/RAVE_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022033">
        <property type="entry name" value="Rav1p_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13950:SF9">
        <property type="entry name" value="RABCONNECTIN-3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13950">
        <property type="entry name" value="RABCONNECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12234">
        <property type="entry name" value="Rav1p_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RAVE complex protein Rav1 C-terminal" evidence="2">
        <location>
            <begin position="530"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1211"/>
            <end position="1230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1334" mass="153018" checksum="1B7663C22DE84350" modified="2017-01-18" version="1">MSYLQTIYLDSDAHAVDINHYNGNIAVAIGSQVSIYEPMSEFMKIPRWQYATKLIDPNNSSLVNTLNWGIQEELIVGSDYLTLWNIRREFGEIHTSILWSMIQSSPVFIAKISQDSSLIASCGKYDKLLKIWDRISFGESSLFDLTYLLHPSSITNLRWKRHDYNNLPKNDEYTNTLYSLGRDSVLRIWSSYNYEKKHIIQHWGSLDLYEETCKRKGRRFVNIVDDYLVTHALRTALGKLYSQELIEIQKRQPDLAIVADENANISVFAIENLSQNPPKLINITKLKSLKFYPKAFVSNPQYINFAETIMDPNGNLSVVIHDLNGVIRHTIINLSFLVDNGTEKDVGMLAHKLTGHKKSIREIVRTIDGEAMLTKSRFNENAIWIPQPLRDSVTLAKKSIVETPAPILKAAILEKGNLLISFCGSALVLWDTSVKIAQQLDSIETHTDEEPLCFTSIPLKKHDHSKHFVAAIYKHESKAWIIKGTKLAETKIQNLPLDEEQVHIIASIDPVGSTYENDRPVLSTITKDGLLQCYSTEIENDEIKWIERDSLDSGIANASYIRGSSIAKFAIVDETRKKFTIWDLKRNVLEYEEIVESEIQDIDWTSTENKQSILAIGFEDFSLLYTQLRYDYTNKNPPFQVIREIDIKNYTTHKIGDSIWLKDGTLVIGTGNQIFVADKKLDLTNDKFTKKSLGSRNIVSNDIMSLCSVLNGPLPLFHPQILIQALFNEKIEIVKEILLRLFLKIREFEIDDKPINELGSTLGFDVTKFFDSHYVKDTTFEEPYTEFNQTVAEGLKEKLTNHSLPYLTRHQQITLVSTIEAVEDIDKNKLSLDSNGLRFALGVKLFQLHRGIQERLTMRDINWAMHSENKELLLQLIENSIKNRRITWSVAREYGMTYWLKFDDLVKTFEVIARNEFSTDDKRDPINCSIYYLALKKKQILLALWRTSVGHPEQQKMMSFLKNDFKEKRWRSAALKNAFVLLSKHRYLLASCFFLLGDSLKDAVNVLIKQVKDTDLAIGVCRVYEGDNGPVLQDMLKKYMLSEAVSLGDRWTTSFVFWKSKQQSRAIQALVKSPIDIIDENEKKDIVITEKSKSFIEDDPLLVILYQNLREKNLNYFHGSLKITPSTESEFILRVASIYMRMGCDYLSVSLLKNWKFLKSEDFKKGQSFTSLKSALNGYNDVSNSSTASKNPGSILEKYGLSPSARRRSSFMSSYQDEGHKDIPSHFSKGDGGNGVGLSLLEKYGLASNSKDKKEINEDEEKKKDHFFSYEVLMQIVTNNNEQLKPITADSTYIDNNAIFKIFENVEFESRPITLKMGMNPLDSTEYFDIPELW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZG5</accession>
    <name>A0A1E3NZG5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Heat shock protein 60, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_64629</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037702.1">
        <property type="nucleotide sequence ID" value="XM_019183777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZG5"/>
    <dbReference type="STRING" id="683960.A0A1E3NZG5"/>
    <dbReference type="GeneID" id="30201023"/>
    <dbReference type="OrthoDB" id="1733909at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006458">
        <property type="term" value="P:'de novo' protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045041">
        <property type="term" value="P:protein import into mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000001">
        <property type="entry name" value="60 kDa chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000001">
        <property type="entry name" value="60 kDa chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000592">
        <property type="entry name" value="PRK00013.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009487">
        <property type="entry name" value="PRK12849.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009488">
        <property type="entry name" value="PRK12850.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009489">
        <property type="entry name" value="PRK12851.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45633">
        <property type="entry name" value="60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="566" mass="60182" checksum="A8A6EC571C26127A" modified="2017-01-18" version="1">MLRASRPVIKRQALSLVRNLSHKELKFGVEGRAALLKGVETLADAVSVTLGPKGRNVLIEQDFGSPKITKDGVTVARSITLKDKFENMGAKLLQEVASKTNESAGDGTTSATVLGRAIFTESVKNVAAGCNPMDLRRGTQAAVEAVVDFLQKNKKEITTSEEIAQVATISANGDAHIGKLLASAMEKVGKEGVITVKEGKTLEDELEVTEGMRFDRGYISPYFITDRKSGKTELERPWIFLSDKKISSINDILPVFEIAVKTRRPLLIIADDVDGEALAACILNQIRGQVTVCAVKAPGFGDNKKNTLGDIAILTGGTVFNEEIDLKPENATPELLGSAASITITKEDTVILNGEGLKENILERIDQIKASVANDETSSYEREKLKERLAKLSGGVAVVKVGGASEVEVGEKKDRYDDALNATRAAVEEGILPGGGTALLKASRVLKDVKAENFDQKLGIDIIRKAITKPARKIIENAGEEGSVIVGKIIDDFGEDFNKGYDSSKGEYTDMIASGIIDPLKVVRSGLVDASGVASLLATTEVAIVDIPEPAPAGGAPAPGGMPGMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZH1</accession>
    <name>A0A1E3NZH1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_95182</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037204.1">
        <property type="nucleotide sequence ID" value="XM_019186485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZH1"/>
    <dbReference type="STRING" id="683960.A0A1E3NZH1"/>
    <dbReference type="GeneID" id="30203731"/>
    <dbReference type="OrthoDB" id="269151at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01856">
        <property type="entry name" value="YlqF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45782">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45782:SF4">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="3">
        <location>
            <begin position="132"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="349" mass="39928" checksum="9953060FF700E380" modified="2017-01-18" version="1">MTTLLKKFIPRTKFPNYRVALTDFKGHHAKALAKLDQIAPQLDLVFELRDARAPISTKNVLLDRSLSHKPKIILYSKKDLAKSDIKLLNKWHKDEKFMLISCKSRSDATKIIDIAKLHYQQMNPPPPLGMRMIITGMPNVGKSTLVNTLRAVGMGSTAKVAQTGGQPGITRATSNIIRICEEPPILLYDTPGVFLPRVENSQRMIVLSLVGAVSTHIVDPVIQADYLLFLCNLQNPNLYSRYLKHPSNEVYTLLSKVAKRNNMYNYRLKSYDEKNAAIHFVQMFRNGKLGGKVKFDVETIVKDDIFNYQEYNKQETQRLEEMDIVLNRAKPKDRNTEEDKIARRVNKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZN6</accession>
    <name>A0A1E3NZN6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_84427</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037873.1">
        <property type="nucleotide sequence ID" value="XM_019185709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZN6"/>
    <dbReference type="GeneID" id="30202955"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="265" mass="31538" checksum="11977C48AE1E3447" modified="2017-01-18" version="1">MLLYRVDNIILSNYLISIYLKLDFEYSGMLPKADGSVERIINFKYYVRRCDDSECTLNGLILWAIYKQANLDLRKNREIDEELTRIWKDVLKQDDNVLVETNLKFNREIFDDLCFESNSEYSFIPNMRPNFYYSIHLEDLSNGNYVAASRQYPIFKFRLNKDDFAKFFLSTNVLMGSKEQLLESIGDHELLLFVHDYMRLYKLAAYKNLKRFITGLFKIYKSNVKQISENYRNQRILDYGMISIVSRAKQENVEYVGFFTLVESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZS8</accession>
    <name>A0A1E3NZS8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">sterol 14alpha-demethylase</fullName>
            <ecNumber evidence="17">1.14.14.154</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Cytochrome P450 51</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Cytochrome P450-14DM</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Cytochrome P450-LIA1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Sterol 14-alpha demethylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="33" type="ORF">WICANDRAFT_93230</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="34">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>32-hydroxylanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-oxolanosterol + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75107"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:166681"/>
            <dbReference type="ChEBI" id="CHEBI:166806"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:75108"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>32-oxolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:75111"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17813"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:166681"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:75112"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>a 14alpha-formyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a Delta(14) steroid + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:68068"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:138031"/>
            <dbReference type="ChEBI" id="CHEBI:176902"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:68069"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>a 14alpha-hydroxymethyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a 14alpha-formyl steroid + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68064"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:176901"/>
            <dbReference type="ChEBI" id="CHEBI:176902"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:68065"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>a 14alpha-methyl steroid + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = a Delta(14) steroid + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54028"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:138029"/>
            <dbReference type="ChEBI" id="CHEBI:138031"/>
            <dbReference type="EC" id="1.14.14.154"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:54029"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>a 14alpha-methyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a 14alpha-hydroxymethyl steroid + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:138029"/>
            <dbReference type="ChEBI" id="CHEBI:176901"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:68061"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>lanosterol + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:25286"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16521"/>
            <dbReference type="ChEBI" id="CHEBI:17813"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.154"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:25287"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>lanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-hydroxylanosterol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75103"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16521"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:166806"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:75104"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 30">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="16">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 31">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.154" evidence="17"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037906.1">
        <property type="nucleotide sequence ID" value="XM_019186407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZS8"/>
    <dbReference type="STRING" id="683960.A0A1E3NZS8"/>
    <dbReference type="GeneID" id="30203653"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008398">
        <property type="term" value="F:sterol 14-demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11042">
        <property type="entry name" value="CYP51-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000033">
        <property type="entry name" value="14-alpha sterol demethylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050529">
        <property type="entry name" value="CYP450_sterol_14alpha_dmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304:SF2">
        <property type="entry name" value="24-HYDROXYCHOLESTEROL 7-ALPHA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 30 31" id="KW-0349">Heme</keyword>
    <keyword evidence="9 30 31" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 32" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 30 31" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 31" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 31" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="15" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="10" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="14" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="32" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="32" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="30">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00037887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00038974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00043106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00043156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00048479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00048866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00049163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00049450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="EMBL" id="ODQ58699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="524" mass="60145" checksum="2613B883CC30A54C" modified="2017-01-18" version="1">MAKVAELIEASKDVLTYLYLAFLELPMYQRISTLVLIPFIYNITWQFLYSLRKDRVPLVFYWIPWFGSAIPYGMQPYEFFTDCQKKYGDVFAFVLLGRVMTVYLGPKGHEFVLNSKLADVSAEEAYRHLTTPVFGNGVIYDCPNARLMEQKKFVKGALTRDAFKSYVPQIREEIIHYFQNSKNFKFGERKHGISNVMATQPEITIFTASRSLMGKQMRERFDESFAGYYSDLDKGFTPINFVFPNLPLPSYRRRDYAQQKISSTYMGLIKERRENNDIKDRDLIDTLMKTSTYKDGVKMTDQEIANLLIGVLMGGQHTSAATSAWFLLHLAEKPELQEEIYQEIMKVTGGEDVTYDDLQEMPLVNNVIKETLRMHMPLHSIFRKVKNPLPVPGTSYVVPKGHHVLVSPGFAMTNENYFPQPEVYNPHRWDEDSTSKAAQDSSTVDYGFGAVSKGVSSPYLPFGGGRHRCIGEQFAYVQLGVLLTTFVKTIRWRYPEGRGLPPVDYQSMVTLPSEPADIEWELRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZY8</accession>
    <name>A0A1E3NZY8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_31794</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037973.1">
        <property type="nucleotide sequence ID" value="XM_019181881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZY8"/>
    <dbReference type="STRING" id="683960.A0A1E3NZY8"/>
    <dbReference type="GeneID" id="30199127"/>
    <dbReference type="OrthoDB" id="342264at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035361">
        <property type="term" value="C:Cul8-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990683">
        <property type="term" value="P:DNA double-strand break attachment to nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18437">
        <property type="entry name" value="BRCT_BRC1_like_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053036">
        <property type="entry name" value="CellCycle_DNARepair_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031906">
        <property type="entry name" value="RTT107_BRCT_6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47667">
        <property type="entry name" value="REGULATOR OF TY1 TRANSPOSITION PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47667:SF1">
        <property type="entry name" value="REGULATOR OF TY1 TRANSPOSITION PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12738">
        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16770">
        <property type="entry name" value="RTT107_BRCT_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16771">
        <property type="entry name" value="RTT107_BRCT_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="103"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="327"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="703"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="468"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="812" mass="92244" checksum="CD479CDD78A1EBF7" modified="2017-01-18" version="1">MKKLFDNFKILIIKSVDLPDTEVQSLAEILQQYCVEYEVLENGSSIDKEELIIKEFTHILSNNINFELYSFAQENMIPVSTSDFIYKTVELQRQPPIRPFSPDPKHVLKDIHVCVGGLPLSDKEAIFGGVRALGGCFSDTLNKFVTHLISTDSDEDCCIAVNSIEDCKIKIVLPNWIDDCLKLRKKLDETPYLLEDGGSVIEQQEKLKEMDVINKDAKSIKAQAKFLNNKRFYIGKDLELTQRSHDLIVVLIENSGGVVENKLNDAQFYIGKFRDGDEYRKASRTGLNVGNLNWIYWMVEHQKYISPYLKLLHYPYVRHGMPQMKKFVIASTNYSGDARFYILKLVEALGAEFTTSLKQRNTHLITATPVGAKYNAAKKWGNISVVNHLWLEETYASWKLQSASHPRYSHFPRSLEMSDIVGETPLDIEVLKHFYDDGNSDYSANVIEDSEGEDEHASAVITTAKIASTPDSSAASIRASSGRKAKDKAAAKLHENMEELNYFQKQHRSKDIPMLPQEIEERKRKRALEESEKKKRQSPSSAGSVEAEVKEEQPENKDSKAEAPKPKKAKKSSSPVPTENPYNITAIATGWDVNFNRADTQILHNLGITLHKEFKKNINAIISPKFMRTEKFLTGLSYKLDYIISPLFLQEVISEFKSNPNDLKKLPKVEDFSIDKLNPTSVKELTSISLSKLMNKSKTRINSYDRIFEDMSFNITPKIPGGAATVQKILKAHGAKNVNVIKTVKDLKNLKSLVKSNDESYVLISSEKNHGDKFQTILKDENQHGKVIEWNWVIDSIFNMEINDKKNVLFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0E7</accession>
    <name>A0A1E3P0E7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_80512</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037574.1">
        <property type="nucleotide sequence ID" value="XM_019185077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0E7"/>
    <dbReference type="STRING" id="683960.A0A1E3P0E7"/>
    <dbReference type="GeneID" id="30202323"/>
    <dbReference type="OrthoDB" id="19141at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017102">
        <property type="term" value="C:methionyl glutamyl tRNA synthetase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006418">
        <property type="term" value="P:tRNA aminoacylation for protein translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10304">
        <property type="entry name" value="GST_C_Arc1p_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02799">
        <property type="entry name" value="tRNA_bind_EMAP-II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000199">
        <property type="entry name" value="tRNA-aminoacylation cofactor ARC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053836">
        <property type="entry name" value="Arc1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF33">
        <property type="entry name" value="AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21972">
        <property type="entry name" value="Arc1p_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="6">
        <location>
            <begin position="206"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="129"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="172"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="373" mass="40911" checksum="262BF45059AAFFF7" modified="2017-01-18" version="1">MADLAASFQKLSLAEAEKADASKFPSLNDEQKALESQWLTLSKRFPESIEQLNDSLRSSTFILGNAPSAADVVVFSRTLPIASKWTSKDYASYRHILRWIDLVQNTLVEVPEDSKLKIDFSVEVPREIKEKKEKKDKAAPAAAAPATTTEAKEGEGKKEKKQKGGKPDEETLKKLREEAAKAKKEKKAKAAADQAAKAAAEAVPPNPSLIDFRIGFIQKAVKHPDADSLYVSTIDAGDEEGPRTVCSGLVKHFPLEALQERYVVLVANLKPVTMRGIKSTAMVLCGSDDEKVEFVDPPAGVKPGDKVFFEGFDGTPEKVLNPKKKIWEAVQPSFSTNEALEVIYKQEGKPEAKLTNKEGQSFKVKSIVSANVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0K6</accession>
    <name>A0A1E3P0K6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">GPI transamidase component PIG-S</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_79347</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGS family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038007.1">
        <property type="nucleotide sequence ID" value="XM_019184876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0K6"/>
    <dbReference type="STRING" id="683960.A0A1E3P0K6"/>
    <dbReference type="GeneID" id="30202122"/>
    <dbReference type="OrthoDB" id="28748at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019540">
        <property type="entry name" value="PtdIno-glycan_biosynth_class_S"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21072">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21072:SF13">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10510">
        <property type="entry name" value="PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="468"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="501" mass="57333" checksum="EBA3A9105BE5E8D2" modified="2017-01-18" version="1">MSEEKVDRKKRVEQIINSEPKQSLSTRQFVVLSILALYLFIGFPLWFKLTEIYRAPLPSKFITTLQNNQNFDLKIQREVFVKITDGLKYPDLAEATQIQIDHELTKYSQDPDERLIVDWNVTIRFDEPGPDDYVLELELGDGEGIAVDPAKRETVLFYTLGSVKSNDLPFFVAQTLLYHIFSSEIESFKAKNQRKDINSITYSPMVHLSFKLLTGDGSPINWRIDKALDEYFQPVIGLFQSYVNFTIDSEIKYFTELNLPNATDTIKLSDLSTIVDFSEWDVSSNMYNYPTLNFVLYYPSKSVSPLNFNFDPSKNAFLIPQWGSIILQPEALEPNTLITEDGLRPVLEKFTSELVNLLGLPKHPKTPLIRVDAVKVHTIVSNLIRATDSLSSLLKLSKSLPNISIPKTVLDNVKKALEARQRAVEKVNIERDFDGALLEANKMLKYSEDAFFDREMVQQNFFPQEHKIAVYLPLLGPLSIVCALGLIRVLMELKRFRSIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0P2</accession>
    <name>A0A1E3P0P2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_93345</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037981.1">
        <property type="nucleotide sequence ID" value="XM_019186412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0P2"/>
    <dbReference type="STRING" id="683960.A0A1E3P0P2"/>
    <dbReference type="GeneID" id="30203658"/>
    <dbReference type="OrthoDB" id="441444at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_B">
        <property type="entry name" value="Ribosomal_uS15_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005290">
        <property type="entry name" value="Ribosomal_uS15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00952">
        <property type="entry name" value="S15_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321">
        <property type="entry name" value="RIBOSOMAL PROTEIN S15, BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321:SF26">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ58774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="290" mass="33868" checksum="B7785DA257D35EF4" modified="2017-01-18" version="1">MFTGGLMIKSIGLVSPASQLSSTRSFSTSSTLFVKKSIRYIRANLRREANILKQRKLVQENSKFDPVLGKPNNAFISRLNAEAKEPNVLGKGYKVPEVERLLYGAQEASIKADYTSDGVRQNAAKQREAIFRILNMRNRSAKEALKFKTDLARKEFERFDGDTGSSEVQAAIATVKIHNIYSHVKENKNDNKNTRYLRMLVQQRQRILKYLKRDNPERYFWTINKLGLNDHVVHMEFNMDKRYMEQFKMFGDRVLVKKSQREKERERKEKRLEKKALKMKIRAKETAARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0S4</accession>
    <name>A0A1E3P0S4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_23873</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038286.1">
        <property type="nucleotide sequence ID" value="XM_019181005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0S4"/>
    <dbReference type="STRING" id="683960.A0A1E3P0S4"/>
    <dbReference type="GeneID" id="30198251"/>
    <dbReference type="OrthoDB" id="3981033at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071248">
        <property type="term" value="P:cellular response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071467">
        <property type="term" value="P:cellular response to pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000181">
        <property type="entry name" value="C2H2 type zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF44">
        <property type="entry name" value="ZINC FINGER PROTEIN 271-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="4"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="60" mass="6871" checksum="1C5C15E78B72B35A" modified="2017-01-18" version="1" fragment="single">ERPFVCTVCGKAFARQHDRKRHEDLHSGKKRYECRGVLADGVTQWGCGKKFARTDALGRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0V1</accession>
    <name>A0A1E3P0V1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_105803</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038160.1">
        <property type="nucleotide sequence ID" value="XM_019180454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0V1"/>
    <dbReference type="STRING" id="683960.A0A1E3P0V1"/>
    <dbReference type="GeneID" id="30197700"/>
    <dbReference type="OrthoDB" id="5551751at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040200">
        <property type="entry name" value="Mug57-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28156">
        <property type="entry name" value="FAS1 DOMAIN-CONTAINING PROTEIN YDR262W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28156:SF1">
        <property type="entry name" value="FAS1 DOMAIN-CONTAINING PROTEIN YDR262W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133604" description="FAS1 domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="3">
        <location>
            <begin position="71"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="233" mass="25632" checksum="DDCC347E2620ECF6" modified="2017-01-18" version="1">MKFSSLALLSLAPLVLGKQLYDLDSFKADGGKVIKVDKREETPEQPLFAPPKMEHSNQIYIPEGEQPQSGPTLLTSSLSIMKDISVFASYVRDNVEISSRFNNKKEQSIVFAPTDEAIESLSLKPWQFPTSVEEDSKSEQEIDEITKSNLNDFITSHIIDGTIPFSSLEGADKGVQFVSKNGKNIKLVNDNGDYYVTAVTDGKDQEWIKVLSTTNADNGAILVIGSSLSLPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1C9</accession>
    <name>A0A1E3P1C9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_93249</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037929.1">
        <property type="nucleotide sequence ID" value="XM_019186410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1C9"/>
    <dbReference type="STRING" id="683960.A0A1E3P1C9"/>
    <dbReference type="GeneID" id="30203656"/>
    <dbReference type="OrthoDB" id="2130629at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF1">
        <property type="entry name" value="LOW AFFINITY AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133609" description="Major facilitator superfamily (MFS) profile domain-containing protein" evidence="6">
        <location>
            <begin position="24"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="248" mass="27364" checksum="01ABB7F439D09601" modified="2017-01-18" version="1" fragment="single">MLSKSHEIVVICILISGQLISQAALTQGLSTITSVEEKYEIDNPGELSWTIVAFSLSQCTFGIITGKLGDMFGHKKLFVFGYFILCFWSLITCISYFASSSFFFISRGFQGFGSSFLLPNGLAIVGRHYENCLTKTIIYASFAAAIPIGSVLGSVIGALFTTTEEWFWIYFIMFSLGGILTILSFVFLPTDKFEKDSSNSTFDYLGVVSFVVAIFTLNISFNLAAMDGWQKPYCYILLIVGVFSLASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1E3</accession>
    <name>A0A1E3P1E3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ59306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1E3"/>
    <dbReference type="GeneID" id="30200861"/>
    <dbReference type="OrthoDB" id="3977947at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="163" mass="18148" checksum="864EF15265A177F7" modified="2017-01-18" version="1">MPVFPPVIGISQLAAPALSTVIFTHGVNSWAALPALLEKGIDNTSISFWISKYSEISGRLTVAALLGSIGFGIRAATHLPFESFSRFYYLLGSISSVGYLLFVPYFKYKMRKISEYNETINSESTDPRSDIRFGLKVHFVSSIFDIAGASFFWYGLYNAIHLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1E6</accession>
    <name>A0A1E3P1E6_WICAA</name>
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        <recommendedName>
            <fullName evidence="11">Cytochrome c oxidase subunit 4, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cytochrome c oxidase polypeptide IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_93976</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c oxidase subunit 5B family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ59070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038277.1">
        <property type="nucleotide sequence ID" value="XM_019186436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1E6"/>
    <dbReference type="STRING" id="683960.A0A1E3P1E6"/>
    <dbReference type="GeneID" id="30203682"/>
    <dbReference type="OrthoDB" id="10249250at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990145">
        <property type="term" value="P:maintenance of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00924">
        <property type="entry name" value="Cyt_c_Oxidase_Vb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.11.10:FF:000003">
        <property type="entry name" value="Cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.11.10">
        <property type="entry name" value="Cytochrome c oxidase, subunit Vb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002124">
        <property type="entry name" value="Cyt_c_oxidase_su5b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036972">
        <property type="entry name" value="Cyt_c_oxidase_su5b_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10122:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5B, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10122">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01215">
        <property type="entry name" value="COX5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57802">
        <property type="entry name" value="Rubredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51359">
        <property type="entry name" value="COX5B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602124-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="156" mass="17224" checksum="2ECFE838BD16463B" modified="2017-01-18" version="1" fragment="single">MLSAITRQRLITRLPIRSFSTTRFALNTSKPVKIAQNLSEVNGAESLIGPGAKEGTMPTDLEQETGLARLELLGKLEGIEIFDTKPLDSSRKGTMADPIVVESYDDYRYVGCTGSPADSHIIEWLKPTVDQVARCWECGSVYKLKPVGVPQDDHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1F6</accession>
    <name>A0A1E3P1F6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="2">2.5.1.114</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_12745</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>4-demethylwyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36355"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.5.1.114"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.114" evidence="2"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038287.1">
        <property type="nucleotide sequence ID" value="XM_019180543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1F6"/>
    <dbReference type="STRING" id="683960.A0A1E3P1F6"/>
    <dbReference type="GeneID" id="30197789"/>
    <dbReference type="OrthoDB" id="2387925at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102522">
        <property type="term" value="F:tRNA 4-demethylwyosine alpha-amino-alpha-carboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026274">
        <property type="entry name" value="tRNA_wybutosine_synth_prot_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF25">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038972">
        <property type="entry name" value="Trm12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="7">
        <location>
            <begin position="137"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="412" mass="47392" checksum="31CCD5A3E66E5664" modified="2017-01-18" version="1" fragment="single">DGSLVKSVKTLLSSTGKLDKSLKIQKSAAGFIVPTLYESLDQLDNEIRNNNKIRPQLVERVVKDVETSNVIENSVLKYLKGIKISSQESNILLSKIPKKYTLYPPLLLINNQDTFESQEWEAHFRLYPSEEFYKQLLANFPQITHIAINKPIIEQDVMRRPFNISPLFGDFGPEPTSEMFDNPTEKDFEDAFWCTVVQNGIYQTWAPRYTMFSRGNIKEKARVLSFKDVKNTTVVDMYAGIGYFTLSYLKLGAKVFCFEINPWSIQGLIRGVAKNGFSYKVVSRNEEIEWDDSVQCWIFNESNEFVNERLCQTNQHFNISHVNMGLLPSSRQSWQHTLTLVNTFSTTNTTIHIHENESVDNLPTFMNLTAVELTNLALDDKTEHNMNIIPLHLEKIKTFAPGIWHICADFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1F9</accession>
    <name>A0A1E3P1F9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_79837</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038526.1">
        <property type="nucleotide sequence ID" value="XM_019184959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1F9"/>
    <dbReference type="GeneID" id="30202205"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="172" mass="20088" checksum="2F820D7BBEECE3B8" modified="2017-01-18" version="1">MCHPAIHDPIRQKQLIIFSVLLMICYIIVTRQVFKTHQLLNNIDKPYELYIKYTTEGTTTKIESASTKKSSERRLQSVLVLEEGSREERVLISKNSNENNKVKNEPLHVRDSYWNEGSEEGPNNEYQGEYEVEEEEKWEDINPVYSDDDDYPRVFGTIVVNGIEYTTIVNPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1H9</accession>
    <name>A0A1E3P1H9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCZ1/INTU/HSP4 first Longin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_69698</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCZ1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038546.1">
        <property type="nucleotide sequence ID" value="XM_019184179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1H9"/>
    <dbReference type="STRING" id="683960.A0A1E3P1H9"/>
    <dbReference type="GeneID" id="30201425"/>
    <dbReference type="OrthoDB" id="240546at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035658">
        <property type="term" value="C:Mon1-Ccz1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013176">
        <property type="entry name" value="Ccz1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043987">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13056">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN CCZ1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13056:SF0">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN CCZ1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19031">
        <property type="entry name" value="Intu_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCZ1/INTU/HSP4 first Longin" evidence="3">
        <location>
            <begin position="17"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="570" mass="65212" checksum="E204966D8F544995" modified="2017-01-18" version="1">MDFFNGKIDAQITPASLQFLAITNPSLSKDEEDSINQVLAFISNEQDEEITDNYKLNKIGIIQGINSLIGNFDNNDSLNYIELDNQLIIFDEFESKYQIMLSLNLTQIKDDGVIEHTSQDIAPVEFLIKMIYQGYNDFRLHNGSFGSMSSEIPIFKETLTKWWTIWFKNLPLGLIENGSLKLYPGFKKANSSQLEAVAPSNSTNFYIFNTSRDNPETYGLLHDSQNNLTIESKLRFLNWIEEQDNYTISSQSLILPQVLKNIKLPDDASSSEIVYDPFKLVFNTLSDISRYSGVTGGVNASVNGVSNGFSSINGYLPSWMGGKSQESGVDEDTDERATDNEEDTNKDMGFLIGKVGDDILEKTVWLQLGVKDEEQKFRVVVFKYFELLFITIYEDGYSKLDDVEFYKDLKQEFVEIGQGIKIDEIKEKNFYFMIYDKFEQSISSNLPDIPNFDDDEVNDLKYFHDLSKSQTQSKMLHKEIIKLLEPELDGSDHQLEKLKRTKNGWWIYKLNHQNIKEILIIKKWQLLHKGGAGSADGIRNKILDNSTSILGSIGKDAKLWLEDYLTKNQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1T6</accession>
    <name>A0A1E3P1T6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_22521</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038079.1">
        <property type="nucleotide sequence ID" value="XM_019180949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1T6"/>
    <dbReference type="STRING" id="683960.A0A1E3P1T6"/>
    <dbReference type="GeneID" id="30198195"/>
    <dbReference type="OrthoDB" id="504170at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000399">
        <property type="term" value="C:cellular bud neck septin structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032161">
        <property type="term" value="C:cleavage apparatus septin structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005940">
        <property type="term" value="C:septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044879">
        <property type="term" value="P:mitotic morphogenesis checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14081">
        <property type="entry name" value="STKc_BRSK1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000394">
        <property type="entry name" value="Serine/threonine-protein kinase HSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="5"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ58872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="266" mass="30183" checksum="7CA456DDBE717FE3" modified="2017-01-18" version="1" fragment="single">HVGPWKLGRTLGRGSTGRVRLAKHSETGQLAAVKIVPKSKFQKNENGQESNTGASPYGIEREIIIMKLISHENVMGLYDVWENKGELYLVLEYVEGGELFDYLIKKGRLQEKEAIHYFQQIINGVSYCHQFNICHRDLKPENLLLDKNLNIKIADFGMAALEINSKLLETSCGSPHYASPEIVTGKNYHGSPSDVWSCGIILFALLTGHLPFDDENIRKLLIKVQTGKFIMPNYLSKDAKDLIWKMLKVNPMERIKIQDILKHPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1Z2</accession>
    <name>A0A1E3P1Z2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_30330</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038716.1">
        <property type="nucleotide sequence ID" value="XM_019181721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1Z2"/>
    <dbReference type="STRING" id="683960.A0A1E3P1Z2"/>
    <dbReference type="GeneID" id="30198967"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05474">
        <property type="entry name" value="SAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033876">
        <property type="entry name" value="SAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133621" description="Peptidase A1 domain-containing protein" evidence="9">
        <location>
            <begin position="20"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="10">
        <location>
            <begin position="65"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="99"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="468" mass="51205" checksum="B288B79BD42EEA82" modified="2017-01-18" version="1">MKLLVLFLSTFFIIKSVSCQVVKFDYSKKTQDSNSHTASSPLINKLRKRQDTYQAIGFTDDTVAYYVNLTVGSQGKEILAVLDTGSSDLWFPGPDNTECTNGKPNGPRFVDAPELTSSETNPKESALAELAKDIQNYCSPSNVFNYHNSKTWHSNDTLFKISYLDGSISTGLWGTDNIQVGGIEIPNFSFAVTNSSNASAIFGISFDKSETTYIMNSKPPYTYSNFPMRLKQDGHISKITYSLSSGKPGDFEGTLLVGGVDHAKYSGGLVTVPILSDQTTAVTLSSLGLKTNGSNIETISSTFYKTLLDSGTTTANFPHQLLDPLARKLGSTLTTVKHNSYEYYDVDCSYLGDKNYTFELDFSGKIISVPLSNAVQKQPSNDVCFLKIVSLTDDIIILGDIFLSSAYIVYDLEDQEISIAQANHSSEEDIEPIVTSVPSATKATAYSSTFSKRLPETTTYDVFSTVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P251</accession>
    <name>A0A1E3P251_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GrpE protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_20038</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the GrpE family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038615.1">
        <property type="nucleotide sequence ID" value="XM_019180855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P251"/>
    <dbReference type="STRING" id="683960.A0A1E3P251"/>
    <dbReference type="GeneID" id="30198101"/>
    <dbReference type="OrthoDB" id="201635at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.22.10:FF:000002">
        <property type="entry name" value="GrpE protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.22.10">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013805">
        <property type="entry name" value="GrpE_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009012">
        <property type="entry name" value="GrpE_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="GRPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237:SF23">
        <property type="entry name" value="GRPE PROTEIN HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00773">
        <property type="entry name" value="GRPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58014">
        <property type="entry name" value="Coiled-coil domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51064">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01071">
        <property type="entry name" value="GRPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="199" mass="22602" checksum="01DE90020E44C5A8" modified="2017-01-18" version="1" fragment="single">QARSAAVPFVRFYSEEAKKSEGEEAAKDENAAPQEKDEVTTLKEEIEKNKKDLAVFKDKYLRAVADFQNLQESTKREITKSKELALKSFAKDLLQTADTFDIALNTLEKDAAEPEKHTKEMKDLVEGVKITQNMFNNTLKRHGLERFEPVGEPFDPNVHEAVFEVPQPDKEAGSVFFVQQPGYYLNKRVLRPAKVGVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2B4</accession>
    <name>A0A1E3P2B4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_79862</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038551.1">
        <property type="nucleotide sequence ID" value="XM_019184964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2B4"/>
    <dbReference type="GeneID" id="30202210"/>
    <dbReference type="OrthoDB" id="5415592at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133616" evidence="1">
        <location>
            <begin position="23"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="163" mass="17814" checksum="03FAA653D60E65D6" modified="2017-01-18" version="1">MFTFTLIKQFILALLAISVVQSRPLAEEIDAAGQHGERHSEPFGLVAIRSGSPLQYQFAVVSHGELFLSGNSSDPYTFAGSLFWDGSLKVLPDLTRFQPLGEGKDLFLSADNSSSLITSPKSDTSFAIKGGDLTYKGVSGFYAVPDNRYGYQYSLKTRISWTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2B9</accession>
    <name>A0A1E3P2B9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exocyst complex component EXO84</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_79916</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EXO84 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038607.1">
        <property type="nucleotide sequence ID" value="XM_019184975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2B9"/>
    <dbReference type="STRING" id="683960.A0A1E3P2B9"/>
    <dbReference type="GeneID" id="30202221"/>
    <dbReference type="OrthoDB" id="642193at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1220">
        <property type="entry name" value="Exo84p, C-terminal helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1210">
        <property type="entry name" value="Exo84p, N-terminal helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033961">
        <property type="entry name" value="Exo84"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032403">
        <property type="entry name" value="Exo84_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042561">
        <property type="entry name" value="Exo84_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042560">
        <property type="entry name" value="Exo84_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21426">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21426:SF12">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16528">
        <property type="entry name" value="Exo84_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25345">
        <property type="entry name" value="PH_EXO84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst component Exo84 C-terminal" evidence="9">
        <location>
            <begin position="492"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="93"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="435"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="197"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="718" mass="81753" checksum="1E7A75D513514260" modified="2017-01-18" version="1">MVELSLRKTRAFKSSDQSSRAKNNPYASLKNNNLGSSNQNVALPSIAKKDQEKIGKLMQRRMSVHQSEVPKMFQQGVGAGGVPALPKDILINHQQQQQQQQQSLPRDALNKEPANYGSNVANLRHQGSRSSKGIDLQDPAVIQLLSDANFNPEQFISSRLNDSTASDIDKFNSNLTTLNKKVTNDIKAVANQTYDKLLNATKELNSTDNELKFLRNAINELSDLTNEMKLQAEKKIQLELDQTNEITKSNSQAKRQNRAKDRSSILVLEKMWASEMSSLFKHVEGAQKYIAAIPGRHVIAESGRWYELNAATFKTLQPAHIFLLNDLILIATRRRKTGQRNKGDGVKQIQSLVADQCWPLREVQLIELKPNGNEDVFTINIRYNSLSYMYQTDRQDHYQKILSGYKKARDELRDISEAENIKQRQLRDSMTLLSISENGANGNGQGTNGSATPKRQSYHNKRNSNVILQDLSTRMHSRSRSMDNSNTLRSLKKIDDMVDESDVTIFHQNFDETLNRLDDLETELTTLKTSCNDEEVLFFNIIKLKISTKKDQVVKLLINSMKSESLTTFEIQTSIKSLLKLNLTDIAKLLLLNNRTSHINSLITKVEESSRLKGTRSISDYIVEVSIVRIQNIKTSIQLYKSLFEETPSNLSYLVEWGVEEIHKHINLLKESLHNVKLPKSTLQDSILIIHRQVHQLKQAGLDVEYLFDEFYRSIDTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2H2</accession>
    <name>A0A1E3P2H2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">DNA polymerase</fullName>
            <ecNumber evidence="20">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">WICANDRAFT_53094</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 20">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="19">Forms DNA polymerase zeta with REV7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="20"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038883.1">
        <property type="nucleotide sequence ID" value="XM_019182705.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P2H2"/>
    <dbReference type="GeneID" id="30199951"/>
    <dbReference type="OrthoDB" id="2414538at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016035">
        <property type="term" value="C:zeta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05778">
        <property type="entry name" value="DNA_polB_zeta_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05534">
        <property type="entry name" value="POLBc_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000007">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000002">
        <property type="entry name" value="DNA polymerase zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056435">
        <property type="entry name" value="DPOD/Z_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030559">
        <property type="entry name" value="PolZ_Rev3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056447">
        <property type="entry name" value="REV3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025687">
        <property type="entry name" value="Znf-C4pol"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812:SF1">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24055">
        <property type="entry name" value="POL3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24065">
        <property type="entry name" value="REV3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14260">
        <property type="entry name" value="zf-C4pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 20" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 20" id="KW-0235">DNA replication</keyword>
    <keyword evidence="15 20" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12 20" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="13 20" id="KW-0408">Iron</keyword>
    <keyword evidence="14 20" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 20" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17 20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 20" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase zeta catalytic subunit N-terminal" evidence="26">
        <location>
            <begin position="21"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase delta/zeta catalytic subunit N-terminal" evidence="25">
        <location>
            <begin position="77"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="23">
        <location>
            <begin position="560"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="22">
        <location>
            <begin position="817"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="domain" description="C4-type zinc-finger of DNA polymerase delta" evidence="24">
        <location>
            <begin position="1283"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="1299"/>
            <end position="1326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00066055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF14260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF24055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF24065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="ODQ59676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1371" mass="157988" checksum="C4A6A6DB1A3C058E" modified="2017-01-18" version="1">MQRRDREDFYVTGTNSSSGDEFKVQVNTYDTYQATPSIFDRSGPPNGDESYSYYQVPVIRVFGSIPTGHNILTHIHGVFPYLYIPYDNQDCFKFQKLLEAVMSESLKRKKKEQSDSNEKPSDEDCKLFKYIAHVSICKGVPFYGYHVGYQAFYKIYLLNPSYSNRLSDLLRDGKVNGRKYECFESHIPYGLQFLTDFNLFGCGWLRLNSLFIRTPILYDETAKTPELVDYLSGYQPTDMDRISYSALEIDTCAQNILNRLDLKERNLHHAFVEKFNPPSSDIYVQSTRDLWKDGDYQRKLKGKAEYSTPPSTSRSPNVTWVESDEHSQLFEYAKKLNSTQDDPDFGTFLKKDTWLDDIKTAFDSTIELFYEPELNAEPEKVDSSGADTDLQKRHGLDSIDESAESNGYFSNDSKDDFTLNDISDIQLTQKLTTKFSLKRRYSVAFDIPRLNEPPKGNFKKIGSLRYSYNKPPPENLDFEAIGLPTINYDDPFYMNEVPKPFVYGGKKFKLGSKKLGNLGEIEVFGESISKDIQNQLKSSLDCFKKWRYTKEPPSYKQVKEYIHESQIKSQIGVTQRHKYAVPKTNERRPDGSNHMTVLIVEVHVNTRGEFKPDPKMDEVSTIFWRTMNGSSYEEGALMFNCPQVIPNIKCRVFSNELVMFEEFADFVRNFDPDIIAGYEVNSSSWGYLIDRSRAIYEFDFVPELSRVHFKSHSKMGDHWGYTHTSAIKICGRHVLNIWRPLRSLNLLKYTIENVSYHALHERVPHFTNKTLTEMFSQDFLSLASHFLKKLELDARLIETQELITRAAEEARLIGIDFYDVYYRGSQYKVESFMVRMAKAENFMLSSPSKKQVRKQKPLECIPLVMEPISAYYKSPLIVLDFQSLYPSIVIAYNYCYSTLLGRLRNYSKNSNQIGTGYVKHPPGLLKLLENDINLSPNGLMFAKSSVRKSLLAKMLEEILDTRFMVKSTMKFLDENLKQLYHSRQLALKLIANVTYGYTSASFSGRMPCSDIADAIVQTGRETLDRAVDLIENEASWGAKVVYGDTDSLFVYLPGRSRDDAFKIGKEMADAVTKSNPDPVTLKFEKVYHPCVLMAKKRYVGYSYENENSPVKFDAKGIETVRRDGHPAQQHIVEQCLRILFETNNVSKIKEFVQDQFNKITYNKVSVQDFCFAKEVKIGSYKQPPPGAVVSTKKMEQDARAEPQYKERVPYVIIQETGSILRERARSPEDFLKNNYKLDAEYYIVKTLIPPLERIFNLLGVDIKPWYTELPKPAKKGSIYNSLCLSCSIPTRSNLCQSCQKNELKTILNLQQRMKTIENRMKDLLVVCRSCSGHSVACESHDCPVYYARVKEGNKLKEAIDVKKRLANVLEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2K1</accession>
    <name>A0A1E3P2K1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_79653</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038329.1">
        <property type="nucleotide sequence ID" value="XM_019184928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2K1"/>
    <dbReference type="GeneID" id="30202174"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="547" mass="62473" checksum="F97E2A17378FFB63" modified="2017-01-18" version="1">MNNAAVQHNASVHDVNRKRVKVVHHIAQGVVRHLHEYREVDKFLDGYKECFGVAPRGSFTSWNQYNPLKLYYCTNVCSAWKRRVRVGGEPKLIPKVEVLCPLCHPFNERDERLGVVKKPIFYDTLTDEYLKHMGTKHGVSPSFNAELPFIGFVVNHKSYRLHAICPFKDSNEPCLKMSGFCHNADGNLLSGYYLHIWLHHIKNDHSSDSEVYQPTVCVNVDGQSIMTNNYFIPLDAGYCLQTLGEPCDSSRLGPFRTCQNILLSDEIADMLLQDLEETDPHYLRHILQTPDYIMKLILSEDYANQQNSTPTNPPQRETLQIAPAPVTTHNNNVLSGQNLPRDPPPYSSPAQHQSALSSDPNSNAKQSTQDPSPPFESSSPPSSLPSPSPPRPSRKSYSPADKPAPLPQNKFHAFTEAEVQSKLKEYVTNISRIIIPSEMTSMLESTRDTADEEDEVSIDEEEEQFPVIEPIESPDKPFIFHDFEQYRHFKELHQELIDECIWKMRMQYQLPFLLSQLNEYSPVSNNSSECHLDEWARCMTKRGILDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2W7</accession>
    <name>A0A1E3P2W7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cullin family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_104874</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2 3">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039036.1">
        <property type="nucleotide sequence ID" value="XM_019180431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2W7"/>
    <dbReference type="STRING" id="683960.A0A1E3P2W7"/>
    <dbReference type="GeneID" id="30197677"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin family profile" evidence="4">
        <location>
            <begin position="408"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ59829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="756" mass="88463" checksum="22678BB266614974" modified="2017-01-18" version="1">MSSRVIRSHSKGSSKSKPLSPYLNTVYDQLNEQIDRILGDFTPTAKVTISYQKLYKFVEFLCRDKMQSKIAATLFEKLDAFINKIADIIYQAYWDLGHDELVGRFVELATIIDVKVTALEKIHLYLDRTYLLNHPAKKTIVRHAMCTFVESCAGVDNGNGDEYKKDVLYQVVMEFTFILGKLRDGNLHVEPLLLRSYHYLHKIFYLTQRNDYLDNEIFSRQEDFYQEEQDAIITCRDTSPELPDFIVARCDSADEIFDTMYKMILRELQFWGKTDADDKFKLKIKEYAVENLIFHDFDKQAVQFVKPLFVSKNYLGVSRMFKFVINSSTSISSNKYLKTFLNIWYSYVCDYLHLLLKDTQQDMVEKLIKAKKNLTLIMQKYMDSNSEVEFKLREAFRTSLHSSNKDTEILSKLLKYIDNFFKTVDPSNTEDNLKVVDDLYLIFKSVKTKATFIKQYQRDLSRRLIIGVSKDKDLEKSLISKIRNEVGDEDSKPLLTMFDDLQINETVASSFKEKLSTVEANSFTGFDPLVLNSKAWPIPKASIKIPDEMNDLMTRFQKFYLETHDKRKLNWCPALSHMTIGAHFPKGDKELQVSSFQGIVLLLFNDHDKLSFNEVKEMTELDSKSLSSILYSLTSGRYKILKKDSAGIYSMNDEFEDRKKSIKVKQIQMKLKNGEDEDVWMQEVDDNVFKADRDEITKAFIVREMKSEKEMDHDVLMKKTVDRFVVESAEVKRIIQLLLDSEYIERIETDRYRYIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P323</accession>
    <name>A0A1E3P323_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_63783</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038494.1">
        <property type="nucleotide sequence ID" value="XM_019183612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P323"/>
    <dbReference type="GeneID" id="30200858"/>
    <dbReference type="OrthoDB" id="4029988at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035283">
        <property type="entry name" value="Fmp23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17315">
        <property type="entry name" value="FMP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="164" mass="19599" checksum="DE425740DBBC3E4F" modified="2017-01-18" version="1">MLSLFKTPSSRLICHVSRNFKPFLISTRKASAISDRVIFPKQKLIKEQTEYKQLPDDSMYIEKFYDELDQFKKEIETNKSITQSNFSDFEKDPNELIQQLELFIEGKIKPQYVEKVMSKFQMERFDEFLRNVKITLILNGGHPMIFDILLQSKAQFDWFVKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3S3</accession>
    <name>A0A1E3P3S3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_78686</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039272.1">
        <property type="nucleotide sequence ID" value="XM_019184773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3S3"/>
    <dbReference type="STRING" id="683960.A0A1E3P3S3"/>
    <dbReference type="GeneID" id="30202019"/>
    <dbReference type="OrthoDB" id="3979490at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="406"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="474"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="516"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="26"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="542"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="542"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="556"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="565" mass="62515" checksum="F236F2263B5E1BCE" modified="2017-01-18" version="1">MGFNIAETLSKGKNVVPMEKRNKTLAYFLVLIATCLDNLNVAGTLTSVFSVAERFDASTTTVSWVFSSYALTLGAFIIIAGKLADVWGPHNVFLVGLFFTSLFALISAVIVKQIIALIVFRALQGVFAASLIPSAFSLTGNYFKGDALLKALAWFIMALTSVFGIGVILGGAFSVSSVGYKGLYYFTFAVGMACFIALYFIIIPIEKTQGHQNLKIKNLDYGGSAFLVIGLLLIILGLTEGGESWHKASSYVPLVVGVLVFFGVVIFEMWYIQGFKDKVNSQINNIEGNTTHFGNEQIKENQDDVCNGSIKTTSESPGTNDWRYKIELLFPREVIKIPNFFQYLSATFLFYVNSISVMSTVVQYSQYVELDSPIMAALKVLPLTLGLSFAALTYRQNLAQRVGTKFIICCTPIMVLTMSVWMSRLDFRVKNSYWKYECISQFIFGFGTNVYFQVYWSDIITGTPLHLQGVVSGILQTAGQIGICFGNTIIAALVGDLKYTRDYDARVELHHKFKKNFYLAYAGSAALFFVLLTVKGKKSVAAKDDVENQPKDQDLETNQDTSNTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3U0</accession>
    <name>A0A1E3P3U0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cation-transporting P-type ATPase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_42271</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039127.1">
        <property type="nucleotide sequence ID" value="XM_019182568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3U0"/>
    <dbReference type="STRING" id="683960.A0A1E3P3U0"/>
    <dbReference type="GeneID" id="30199814"/>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140567">
        <property type="term" value="F:membrane protein dislocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140569">
        <property type="term" value="P:extraction of mislocalized protein from ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034214">
        <property type="term" value="P:protein hexamerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055092">
        <property type="term" value="P:sterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07543">
        <property type="entry name" value="P-type_ATPase_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000049">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000071">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057255">
        <property type="entry name" value="2TM_P5A-ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047820">
        <property type="entry name" value="P5A-type_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF7">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE HELIX TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23143">
        <property type="entry name" value="2TM_P5A-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01229">
        <property type="entry name" value="COF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="27"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="219"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="398"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="993"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1018"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1055"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1134"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1174"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="domain" description="P5A-ATPase transmembrane helical hairpin" evidence="14">
        <location>
            <begin position="22"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="13">
        <location>
            <begin position="256"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ59920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1208" mass="134685" checksum="8A188F092E402FEE" modified="2017-01-18" version="1">MADNLVDNKAVKSSQLLRPKVLTQRPYVFPFLVLYPWFFHIYFNNYDEYIKGSEWTFVYLGAIISLQMLLLLMPNWNINIDALFNYSKVKSLDEASHILIVAAPNNGLGAIAEISRDKYPDEIQTSFVFQKRRFIYHHEDASFTPPRFVVDQEPKLSEFQQSKGISGDISKLERLYGKNTFDIPIPTFWELFKEHATAPFFVFQVFCVGLWLLDEFWYYSLFTLFMLVSFECTTVFQRRTTMGEFRTMGVKPFNIYVYRDNKWTEISTVDLLPGDLVSITRTGDDSAIPCDLILTDGSAIVNEAMLSGESTPLLKESIKLRPAHENLQLDGLDKNSVLHGGTKALQVTAPTDPIVQLAPDNGALAIVCKTGFETSQGSLVRVMIFSAERISVGNYESLFFILFLLIFAVAASWYVWVEGTKMGRIQNKLILDCILIVTSVVPPELPMELTMAVNTSLASLSKFYIYCTEPFRIPLAGRIDVCCFDKTGTLTGEDLVFEGLAGFGDSIRHLSKPSEVPDITNHVLGSAHALVKLDDGEIVGDPMEKATLEAVGWKIGEKDIVFRETSKSKTEKIKILRRFQFSSALKRSSSISTVGQKTLVSTKGAPETIRERLTKVPENYEEIYKSFTRSGSRVLALAYKYMEDNISPAKVTKLDRDAVETGLIFGGFIVFHCPLKEDAIETIEMLNESSHRSIMITGDNPLTAVHVAKEVKIVEREVLILDAPEEHHGDHELVWRSVDESKIIPFSSSDKIDESTFKTHDIAITGYALAKLLNHPQLHSLIRHTWVYSRVSPSQKEFILTTLKHLGYNTLMCGDGTNDVGALKQAHVGVALLNGTEEGMKKMAEARKIDSLQKVYTKQCDIMERWGKPHPPVPLPIAHLYPPGPGNPNYLKAIEKQGITVTDEMRELAQKSAVVSTTVAQGKAPNQAQSELADKLLGSLNQLEEDSEAPTLKLGDASVAAPFTSKLANVSTVTNIIRQGRCALVSTIQMYKILALNCLISAYSLSVIYLAGVKFGDGQATVSGLLISVCFLSISRGRPVEKLSKERPQPGIFNIYIMGSILGQFAVHIATLVYITAEIYKLEPREPKPDLEKEFAPSLLNTGIFLIQLAQQVSTFAVNYQGLPFRESIKDNKGMYYGLLGVAGLALAGSTEFIPELNEAMKFVPMEAEFKTKLTTTIVLDLVVAWVIEVVLKYFFMDSKAADIAQRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P449</accession>
    <name>A0A1E3P449_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_83746</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038864.1">
        <property type="nucleotide sequence ID" value="XM_019185588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P449"/>
    <dbReference type="STRING" id="683960.A0A1E3P449"/>
    <dbReference type="GeneID" id="30202834"/>
    <dbReference type="OrthoDB" id="760868at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000244">
        <property type="entry name" value="Nonsense-mediated mRNA decay protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF18">
        <property type="entry name" value="D-IMPORTIN 7_RANBP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="7">
        <location>
            <begin position="24"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1022" mass="116576" checksum="6986A8DF99A53C6D" modified="2017-01-18" version="1">MDVNALYQTFSESLSPNQDIRHQAEQTLRRAEKSIGFLGACLDILSTEGVDIAVKKAAAVFFKNRITRYWNRDDKSDEGRIDNDERPIIRDRILPTLVKVNYQLRNQLESVLSTIIVGDYPSKWPTLVDSAVQLLYAQNLDSAYTGLLCFAEICRTYRWVTNEERKELDQLIISHFPTLLSVADTLLKEESPDAGEMTKLILKVYKFSTYHDLPEPLQTPEAITSWMNFHVSVIQKELPGYIYSSMEDSERKLDPWVKCKKWAYANLYRLFTRYASHSLSKKFSYTKFNDFFTENFVPQLLNLFFTQIDQWCNKILWLSDESLYFLINFIENAVTQKKTWPFIKPHFELLVSHFVFPLLCPSDDILDRFENDPQEYIHTNLDIYDESTSPDLAAISLLVTLTTKKKKTTLEPILKFAYNSLNQLKDVAGTDLEAAKKLEGAMRLIGSISDNFVKETSPYYSQMEAFLTNLIFPHFDSQFGFIKARTCEVASKFAEIEFQDAANLGVLFRGILSSFENDHLPVQLEASLALQNFIKVPQFQEALTPVILPTMQRLLTLSNTIDSDATSGVMQEMVEVFSEQLQPFGVELIGNLVEQFLRLAKELNDAANVDVDNFDAGYDDLTDKQMAALGLLNTMITVLLSFESSVDVVYKLEETFAPAVEFVLRNNIEDFFKEVSELIENSTFLVRQISPVCWKLFEILIPQINGGIALLYLEDWMPTLNNYLVYGAPQISANQAYSGALFNLFKTIMTSDDNSVTDFAYAGEIAQKLILALEKSSNLYLEEILQITTDNLINAEEMVNLYSINMIDTIVSALVYYPVETLKVLESKNFVLVFFKLWFQYIPKLTRVYDLKLSTLGLLSLINVQSNALASSQMIEVMSEVGVMLTVLMEKLPKAIQDLEKRRKDYDGELDGAGEFEYGEDDEEEWENEDVDEDADAAAQDDYLEFLNAEAAKLKNSGFYDDAEEDVIEDPLASTILDGLNVFATFKEGILSVQANDPQRYQVIFQKLNAEQQSTINDVIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4J3</accession>
    <name>A0A1E3P4J3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Seryl-tRNA(Ser) synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_100555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039616.1">
        <property type="nucleotide sequence ID" value="XM_019180299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4J3"/>
    <dbReference type="STRING" id="683960.A0A1E3P4J3"/>
    <dbReference type="GeneID" id="30197545"/>
    <dbReference type="OrthoDB" id="10264585at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAP" evidence="2">
        <location>
            <begin position="5"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="435" mass="49941" checksum="A894EE3ABE2DF078" modified="2017-01-18" version="1">MEFHLNEYTLEALKICCADIGISTDAKKQEIVKRLADARIALLKKQLHEEESEQGSESMYQSADEDDKLVSKEEFKTLESAFYEQNALNKEQNKIQKEIGLKFKAKEDASELVKKKDEIEQDADKQLKFKVNQIGNIAHESVVISNDEDNNELVRTWKPEGSADIAKLSHHEVLSRLDGYDPERGVRIVGHRGYFLRNYGVFLNQALINHGLSFLAENEYTPLQAPVMMNKDVMAKTAQLSQFDQELYKVIDGEDEKYLIATLEQPISAYHAGEWFESPAEQLPVQKSWTEFDCMIGLSEEFYKFLRLPYRVVGIVSGELNNAAAKKYDLEAWFPFQQEYKELVPCSNCTDYQSRNLEIRSGIKKMNVDREKKYVHCLNSTLCATEGALCCILENYQTEDGLVIPEVLRKYIPGEPEFIPFLKELPKNSTSQKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4J5</accession>
    <name>A0A1E3P4J5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_62765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039407.1">
        <property type="nucleotide sequence ID" value="XM_019183432.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4J5"/>
    <dbReference type="STRING" id="683960.A0A1E3P4J5"/>
    <dbReference type="GeneID" id="30200678"/>
    <dbReference type="OrthoDB" id="6252103at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052779">
        <property type="entry name" value="WDR62"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45589">
        <property type="entry name" value="WD REPEAT DOMAIN 62, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45589:SF1">
        <property type="entry name" value="WD REPEAT DOMAIN 62, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="717"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="717"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="754"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="768"/>
            <end position="827"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="896" mass="99848" checksum="C48A37510B43BD1A" modified="2017-01-18" version="1">MSADSKLSIERIYGTSSNNQLNFAKSKNLVAYIASGGVVVAPIDKSTGTLQSNQRFFCAYNANNQSSNQTGANSYLNLFQNQQPSEEKDQYGNPKLQGTITINNSTDINTQMNDLSLSSSPPKQAQKIKTITCIAISPDEKLLAVGETGSQPRILIFSLAPDSNDHPILSLSEHNFGILAVRFSPDSKYLLSVGVNHDSFLYLWHLDGANTAIVGANRCTSQINGLLWIDQHIVTFGIRHIKIWKFEKFETKSMIDGKSVILGDFLNGNFVHATGDDIDDLLFLTATGEICLYDPAKNALSLRYIFKDQDEKVGAILRDAYNSRVWFGSDSLDSLSPESLEKEIQYETGGCPDSPSKVKFYSKIAAIYEVNEQFLIYLTSDEEIQLFDKTTSTTKPLIDTLSKSITGVKSVSNGKIVNWTKEGVVKVIGQDNLDIDFLVKVDINDLPNKIISNHVTALDVNDSNEIIIGDAYGNLSTFDESGQMTFSTPTHEFTVNDVCYFRYEGFEIIVSIGRDRMIQVLAKKTCEEDDTANNSDIGSQWSVFQTLDDHKGNLLDLKHHDGRIFVSSADRSITIHKFFVDEEGLSIEKEKTISVKSSPLAMTLYKEDLVVSTNDKNITVYNTTTLESTRSFKLYDNGNESLLVDNVQVTDDNVMVCSCSDKSIRSFSYLTGKQLSVNWGHSEAIKQLVLLSKNHLITLSNSGCLFTWSLNNKSPFNQDSSKSTIQKYSDFTTPPKVTRKIKRPLTASPSTRKAASSSVTRSPVTSRLQTTARLTPSKLTNSTKTNLSPSSTKSSPRQTSPKASNTPTTPTISRFNPRSSMSVNNRSRISELSERKLRAEDMVTYLKEFKENIGTYSVAEVETVKKEIKELFNYEEELIIKYNTQMMDKLNELMKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4L2</accession>
    <name>A0A1E3P4L2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_79024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039633.1">
        <property type="nucleotide sequence ID" value="XM_019184823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4L2"/>
    <dbReference type="GeneID" id="30202069"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="259" mass="29636" checksum="3AE168A56378630B" modified="2017-01-18" version="1">MSSRRVGITMNPERSGSERMVRSKLELVLFMASFSQRATGFKIVKEVKDQNCRIIYFDDGGPIQAIDLGTDEDFILNIDTHQGKIPVEVKYSKYFKSLPLPVSEKFLDNEADEISDAFLNYCMQEIMRKTTEFNELEHGKGSGINHRSNDEDALSYYEMKRFYTQLVTPWFCQKYIPKRIVEKLSSSESEEDEEEEIEDVKNKKSNGKSKNNKKEGKKKVEEPLEDYTQGPIIYPDIPSGGKSGALFGIDKGHPFGRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P503</accession>
    <name>A0A1E3P503_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytoplasmic tRNA 2-thiolation protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">NCS2</name>
        <name evidence="3" type="synonym">CTU2</name>
        <name evidence="4" type="ORF">WICANDRAFT_61113</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CTU2/NCS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039748.1">
        <property type="nucleotide sequence ID" value="XM_019183110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P503"/>
    <dbReference type="STRING" id="683960.A0A1E3P503"/>
    <dbReference type="GeneID" id="30200356"/>
    <dbReference type="OrthoDB" id="25129at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03054">
        <property type="entry name" value="CTU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019407">
        <property type="entry name" value="CTU2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20882">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20882:SF14">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10288">
        <property type="entry name" value="CTU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="440" mass="50398" checksum="3A8997A99B8AF1B2" modified="2017-01-18" version="1">MSVQAELPELGNCSRCKTEKAVAVSRKEAFCKSCFIRFISGKQRKQMSDEKFKVKFGEHIVPEKVLYPISFGPSSIALLDILVSLYIEQLRNPRAKLGFELTVLYVDDSSINGYENDVDAIIQSIKTRYNSDQYSITFKKLDIDSFIGNKESLHKVTLLQNFDCFKIKKDFSELKSVNEILDVCPNRASKHDLIQIIKKELIKNFAISNDHKTIIWGHNMSRLAEEVIALTVKGRGAEIYSNLTDGVESYKEWNLEHLHPLRDVSQNEINMFINLQELSDLLLIPKDKSEKLMVKQKTINEIVADYYKVVDTGYDNIVSTVVKTGAKLVEPKKFGEKYCSICKGKIYNDPMNWLREITYNKSVSPENEIEESSLQEWKEANKDEIIELESSEKLSVDICYGCSVTLGGTKKSSLNWPVPDHERTKQEILDEFIIDNDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P528</accession>
    <name>A0A1E3P528_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2-methylcitrate dehydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_84324</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PrpD family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039680.1">
        <property type="nucleotide sequence ID" value="XM_019185690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P528"/>
    <dbReference type="STRING" id="683960.A0A1E3P528"/>
    <dbReference type="GeneID" id="30202936"/>
    <dbReference type="OrthoDB" id="10055203at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047547">
        <property type="term" value="F:2-methylcitrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019679">
        <property type="term" value="P:propionate metabolic process, methylcitrate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.120:FF:000001">
        <property type="entry name" value="2-methylcitrate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4100.10">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.120">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012705">
        <property type="entry name" value="2Me_IsoCit_deHydtase_PrpD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036148">
        <property type="entry name" value="MmgE/PrpD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042183">
        <property type="entry name" value="MmgE/PrpD_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042188">
        <property type="entry name" value="MmgE/PrpD_sf_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005656">
        <property type="entry name" value="MmgE_PrpD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045337">
        <property type="entry name" value="MmgE_PrpD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045336">
        <property type="entry name" value="MmgE_PrpD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006943">
        <property type="entry name" value="PRK09425.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02330">
        <property type="entry name" value="prpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16943">
        <property type="entry name" value="2-METHYLCITRATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16943:SF16">
        <property type="entry name" value="2-METHYLCITRATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19305">
        <property type="entry name" value="MmgE_PrpD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03972">
        <property type="entry name" value="MmgE_PrpD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103378">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MmgE/PrpD N-terminal" evidence="3">
        <location>
            <begin position="19"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="MmgE/PrpD C-terminal" evidence="4">
        <location>
            <begin position="289"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="54133" checksum="8E2A0D417FB7C02A" modified="2017-01-18" version="1">MSSIITENTRPEPDKVLKDISSYIHEYKVDSKLAFDTAKLCLLDTLGCGLAALKYEQPKRIIGPVVPGTVVPNGTKVIGTSHQLDPVRGAWSIGTLIRWLDYNDCWLAAEWGHPSDNLGGILAVADYLTRAKKNGVEGQIFTVKDVLEGMIKAHEIQGVLALENSFNKVGLDHVLLVKVATTAVVSKMLGLNYDQTIDALSQAFVDGQSLRTYRHAPNTMSRKSWAAGDAVSRAVNLAYHVKNGEGGMPSILSAKTWGFYDVLFKGNEFKFQRPYGSYVMENVLFKISFPAEFHAQTAVEAALTVNKKLSELGKSYKDIKSVKIRTQEAAMRIIDKKGPLYNYADRDHCIQYMVAIPLIHGRLTADDYSDSIASNPDIDALRDKMYCVEDKQFTVDYHAPDKRYIGNALTVELNDGTTLDEVVVEFPIGHRKRREEGKPVLLNKFERHVSEHFNDPSKVDKILKASYSPDFEKMDIDEYVDLYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5I3</accession>
    <name>A0A1E3P5I3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Beta-catenin-like protein 1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_77388</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039918.1">
        <property type="nucleotide sequence ID" value="XM_019184549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5I3"/>
    <dbReference type="STRING" id="683960.A0A1E3P5I3"/>
    <dbReference type="GeneID" id="30201795"/>
    <dbReference type="OrthoDB" id="1898821at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039678">
        <property type="entry name" value="CTNNBL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013180">
        <property type="entry name" value="CTNNBL1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14978:SF0">
        <property type="entry name" value="BETA-CATENIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14978">
        <property type="entry name" value="BETA-CATENIN-LIKE PROTEIN 1 NUCLEAR ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08216">
        <property type="entry name" value="CTNNBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01156">
        <property type="entry name" value="DUF1716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta-catenin-like protein 1 N-terminal" evidence="7">
        <location>
            <begin position="41"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="542" mass="63453" checksum="6ADAC7839A3AE266" modified="2017-01-18" version="1">MSDYKRRKIDESGGGGGSLIAANRAAKEQQQQQEKILQYLQRTTAQDGLNAADNDDVVMEPEVYDLNWLENLSKELKTTVQQNSDVRIKYSNDPLKFLDSETKLNEIVKQISNITLAELPLYNDFIRLSGESLSQIITHPNGDIINQFIIVFNDLIDHEYEQDQSILLQFIKTQNIVNFLLEYLKILIDQDDEDSKDVQITILTIFNELTNYEDDELRKQLIEFDQLTQFFNDSVRQISTVEGISYYDELVTDYLFKIVDWDKLRFVAKHDSAIEIFLIQLSHFTEIQPEEGSTQENFVESIISILSMVLTSKDGRSKFLQLEGFELLLILIKNGDWGFKTFVKLSNYLIESINDTNNVELSVSIIESNFFLNNVFKILKQNHKDRDEIYQILKILVKLIIFLPFNSDQRIRLINKLVNKNFKSLNVLLKIESKYDQNVSKLDVNSEQTYSFDDQYLEKIENGLDILQQISIFKSWLLIEDEKLKNDMIHMKHFNLEKLKNILNGYKDELKFNIENTEDDVTKNEFFEYINMINELLANLYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5K6</accession>
    <name>A0A1E3P5K6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_28985</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAPT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039767.1">
        <property type="nucleotide sequence ID" value="XM_019181566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5K6"/>
    <dbReference type="GeneID" id="30198812"/>
    <dbReference type="OrthoDB" id="5376140at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008010">
        <property type="entry name" value="Tatp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317:SF4">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05346">
        <property type="entry name" value="DUF747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="401"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="433"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="544" mass="61897" checksum="7E5F5DB325095245" modified="2017-01-18" version="1">MKSGSKRGRSRTLSIVSGNSHSTSYNLYKLLLKELGIDIRDQKLKTREDDDPERHVGSTLISSMESLEQIMNMIKLPFYLENFMVFGLLSCLNSFLKMLVILPLKIVIDISVLIMKFRNQFIINTNNEGERRRDYLSIINLKNLLFASLLILAMIFSNVDTSKIYHTIRAQSSIKLYVMFGVFEISDKLLATVGQDLLNVLFSYNNVVLTDVGSISQFILFYGLSVIYLTIHTVVLIYQTISLNVAANSYSNSLLTLLLSNQFAEIKGSVFKKIDREGLFQMSCADVVERFQLLTMLSIISLRNLVQIGNDSETVGLIPNSSFASWNKALGIIFGPTAIVIGSELIVDWVKHSYVTKFNKIRPKIYGNYLNVLSSDVIDNFKIRVSNNNDDKIQHRIGLPLPALFVLFVVMSKHSLSWFLFDPTKGSSWSSQLIFTNLIILILSYITLLLFKLLLELILIKWSNLRMKKVALQQQKEPDTETDYVPGLVSGGMGAVDSTSRKLLYDPHETMSPSLNEIRQRKESKTHGNLNKVSRYRMFSKRIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5Q5</accession>
    <name>A0A1E3P5Q5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Pyruvate decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_84167</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039469.1">
        <property type="nucleotide sequence ID" value="XM_019185658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5Q5"/>
    <dbReference type="STRING" id="683960.A0A1E3P5Q5"/>
    <dbReference type="GeneID" id="30202904"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004737">
        <property type="term" value="F:pyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000949">
        <property type="term" value="P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02005">
        <property type="entry name" value="TPP_PDC_IPDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07038">
        <property type="entry name" value="TPP_PYR_PDC_IPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000019">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000024">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047214">
        <property type="entry name" value="TPP_PDC_IPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047213">
        <property type="entry name" value="TPP_PYR_PDC_IPDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF3">
        <property type="entry name" value="TRANSAMINATED AMINO ACID DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036565">
        <property type="entry name" value="Pyruvt_ip_decrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="13">
        <location>
            <begin position="13"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="11">
        <location>
            <begin position="226"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="12">
        <location>
            <begin position="419"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>1</label>
            <note>substrate; ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>1</label>
            <note>substrate; ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>2</label>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="492"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="494"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>1</label>
            <note>substrate; ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ60262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="577" mass="64492" checksum="3E8988507FC1DEDF" modified="2017-01-18" version="1">MTVSETVENVALGSYLFSRLQQVNSLKSIFGVPGDFNLSLLEHLYNFPDLNWIGNCNELNAGYAADGYARVNKFGVLVTTFGVGELSALNAISGSFTENVPVLHIVGTSSTMAKKLNNKTLHHLIPSKNTWEVAQDHYVYEKIVEPFSCIVESLNDDDLTVLQSKIDNVIETIYKSKKPGYLFIPSNLADIQIPVDTTKKLEFNSIDKHPELTNQIVSKIIDKLYKKKFAILADNLIENRKDVLNNFIQSNNIDCFSTVLGKSIIDETFEKFHGVFNGKISSEGAADTFSKFELILHLGVVTNEINNFNNLKAKDFVGDVVELGKDYVLIDDELITSVDGEIIFDSLLAQIEPMKLCTDQSVESWGSKRSYPSSPTLLNATKKLSQSSMAKILESKLREGDVIVCEMCSFLFAIPDIKFPKDSLFISQNFYGSIGYALPSTLGASLAIRDAGLESKRRVILLQGDGSAQMTIQELSSYVRYGIKPTVLLLNNDGYSVERVCCGPTRSYNDIQPNWNWLKLFNVFGDVENKSVNVRVETNHELEMELNKDDGELKMIEVMLDKMDVPWRFSYVAGKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6A1</accession>
    <name>A0A1E3P6A1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Catabolic 3-dehydroquinase</fullName>
            <shortName evidence="3">cDHQase</shortName>
            <ecNumber evidence="3">4.2.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">3-dehydroquinate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">DQD1</name>
        <name evidence="7" type="ORF">WICANDRAFT_61526</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Is involved in the catabolism of quinate. Allows the utilization of quinate as carbon source via the beta-ketoadipate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Aromatic compound metabolism; 3,4-dihydroxybenzoate biosynthesis; 3,4-dihydroxybenzoate from 3-dehydroquinate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homododecamer. Adopts a ring-like structure, composed of an arrangement of two hexameric rings stacked on top of one another.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the type-II 3-dehydroquinase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.10" evidence="3"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040172.1">
        <property type="nucleotide sequence ID" value="XM_019183192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6A1"/>
    <dbReference type="STRING" id="683960.A0A1E3P6A1"/>
    <dbReference type="GeneID" id="30200438"/>
    <dbReference type="OrthoDB" id="8191625at2759"/>
    <dbReference type="UniPathway" id="UPA00088">
        <property type="reaction ID" value="UER00178"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046279">
        <property type="term" value="P:3,4-dihydroxybenzoate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019631">
        <property type="term" value="P:quinate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00466">
        <property type="entry name" value="DHQase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.9100">
        <property type="entry name" value="Dehydroquinase, class II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00169">
        <property type="entry name" value="AroQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001874">
        <property type="entry name" value="DHquinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018509">
        <property type="entry name" value="DHquinase_II_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036441">
        <property type="entry name" value="DHquinase_II_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01088">
        <property type="entry name" value="aroQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003804">
        <property type="entry name" value="PRK05395.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003805">
        <property type="entry name" value="PRK05395.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003806">
        <property type="entry name" value="PRK05395.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003807">
        <property type="entry name" value="PRK05395.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21272">
        <property type="entry name" value="CATABOLIC 3-DEHYDROQUINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21272:SF3">
        <property type="entry name" value="CATABOLIC 3-DEHYDROQUINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01220">
        <property type="entry name" value="DHquinase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001399">
        <property type="entry name" value="DHquinase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52304">
        <property type="entry name" value="Type II 3-dehydroquinate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01029">
        <property type="entry name" value="DEHYDROQUINASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 3" id="KW-0672">Quinate metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="3 4">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3 4">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <begin position="105"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="3 6">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="150" mass="16762" checksum="B079F7916A117F28" modified="2017-01-18" version="1">MVKKVLLLHGPNLNLLGSREPEKYGTTTLKEIEQNASKQVQAHSSDAQIYFHQDNHEGGLIDRIHQAKADGVEFIIINAGAYTHTSVALRDALLGVDIPFVELHITNVHSREEFRHKSYLVDKAIAIISGLGPIYGYKAAIEFALNYERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6C0</accession>
    <name>A0A1E3P6C0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endoribonuclease YSH1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Endoribonuclease ysh1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 11">mRNA 3'-end-processing protein YSH1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_104133</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF2/YSH1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040192.1">
        <property type="nucleotide sequence ID" value="XM_019180398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6C0"/>
    <dbReference type="STRING" id="683960.A0A1E3P6C0"/>
    <dbReference type="GeneID" id="30197644"/>
    <dbReference type="OrthoDB" id="10249535at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16292">
        <property type="entry name" value="CPSF3-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000001">
        <property type="entry name" value="Cleavage and polyadenylation specificity factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10890:FF:000001">
        <property type="entry name" value="Cleavage and polyadenylation specificity factor subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021718">
        <property type="entry name" value="CPSF73-100_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF11">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11718">
        <property type="entry name" value="CPSF73-100_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01098">
        <property type="entry name" value="CPSF73-100_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="12">
        <location>
            <begin position="27"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="13">
        <location>
            <begin position="251"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA 3'-end-processing endonuclease polyadenylation factor C-term" evidence="14">
        <location>
            <begin position="487"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ60985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="711" mass="79380" checksum="AADB7D2762A30493" modified="2017-01-18" version="1">MATKEGESANGAESFRFMNLGGGNEVGRSCHIIQYKGKTIMLDAGVHPAHSGIAALPFYDEFDLSTVDILLISHFHLDHAASLPYVMQHTNFKGRVFMTHPTKAIYRWLLSDFVKVTSIGASASSALYTDDDLSESFDKIETVDYHSTIEVDGIRFTAYHAGHVLGAAMFLVEIGGLKFLFTGDYSREENRHLNPAEVPPTKPDVLITESTFGTATHEPRLEKEVRLTNLIHSTLIKGGRVLLPVFALGTAQELLLILDEYWSQHQDLDGVNVYYASSLAKKCLAVFQTYINMMNDNIRKQFRDQNSNPFQFKHIKNIKNLDKFDDFGPCVVVASPGMLQNGVSRELLERWAPDSRNSVIMTGYSVEGTLAKNLLTEPDQIPSFQNPDNSIPRRINIEEISFAAHVDYEQNSKFIELVDPKTIILVHGESNPMGRLKSALLSKYSKFKGTDQEVKVYNPRNCDELDLKFKGLKIAKALGAIVEEKQTNETLSGVLVSKDFDLNLMKVDDLREYAGLTSTIVKERQTIRVDAGRDLIQWHLSQMFGYVEILIDDKDEFELKLMDEISVNLINGICTIEWTSGIINDTIADSVVAILLSVDSSPASVKLSSKSCDHDHSKDHSHLEDSLADTRVSTRIKRITAMLEAQFGEAFTNNEDGKTAVIKIGKNEAKIDYYDLTVECSSNVLKGRVENVLNRATDLVAPLAQSRKIQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6C6</accession>
    <name>A0A1E3P6C6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">DNA 5'-3' helicase</fullName>
            <ecNumber evidence="19">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">WICANDRAFT_28945</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the helicase family. RAD3/XPD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="19"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040021.1">
        <property type="nucleotide sequence ID" value="XM_019181559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6C6"/>
    <dbReference type="STRING" id="683960.A0A1E3P6C6"/>
    <dbReference type="GeneID" id="30198805"/>
    <dbReference type="OrthoDB" id="272481at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045951">
        <property type="term" value="P:positive regulation of mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18788">
        <property type="entry name" value="SF2_C_XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.40:FF:000001">
        <property type="entry name" value="DNA repair helicase (Rad3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.20:FF:000001">
        <property type="entry name" value="DNA repair helicase rad15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000135">
        <property type="entry name" value="DNA repair helicase RAD3, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000128">
        <property type="entry name" value="Putative DNA repair helicase RAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000381">
        <property type="entry name" value="TFIIH basal transcription factor complex helicase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010643">
        <property type="entry name" value="HBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013020">
        <property type="entry name" value="Rad3/Chl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001945">
        <property type="entry name" value="RAD3/XPD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00604">
        <property type="entry name" value="rad3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HBB"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00852">
        <property type="entry name" value="XRODRMPGMNTD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="17" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="22">
        <location>
            <begin position="7"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="747"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023004"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="ODQ60814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="778" mass="89083" checksum="ADF00E80E79D2887" modified="2017-01-18" version="1">MKFYIDDLPVLFPYPRIYPEQYAYMSDIKKTLDVGGNSILEMPSGTGKTVSLLSLTVAYQMHYPEHRKIVYCSRTMSEIEKALIELHNLMSYRAKELGYVEDFRGLGLTSRKNLCLHPTVSKEKKGSIVDEKCRRMTSGMAKRKIEQGETENVDLCEFHENLYELEPDNLVPPGVYSFDSLTNYCKDQKTCPYFTVRRMIPFCNIIIYSYHYLLDPKISERVSKELSKDAIVIFDEAHNIDNVCIESLSLDLTNDVLKKAAKGANGLSKRIDDVKKTDSRKLQEEYEKLVSGLSAADIINNEQQMLETPVLSDDLLKEAVPGNIRRAEHFVAFLKRFIEYLKTRMKVLHVISETPTSFLQHVKQLTFIERKPLRFCSERLSLLVRTLEITDIEDYNALKDVATFATLVSTYEQGFALILEPYETENATVPNPILRFTCLDASIAIKPIFDRFSSVIITSGTISPLDMYPRMLNFETVIQESYTMTLAKRSFLPMIVTKGSDQVAISSRFEIRNDPSIVRNYGSLLIEFAKITPDGMVVFFPSYLYMESIISMWQTMGILDEVWKYKLILVETPDAQETALALETYRTACSNGRGAILLSVARGKVSEGIDFDHHYGRTVLMIGIPFQYTESRILKARLEFLRDHYQIRENDFLSFDAMRHAAQCLGRVLRGKDDYGIMVLADRRFARKKSQLPKWIAQALMDADTNLSTDMAVANAKQFLRTMAQPTDPKDQEGVSVWDIEQLHEFQQQQTSSGVEEVDEDGDQEMLSREEELELANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6E2</accession>
    <name>A0A1E3P6E2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Histone-binding protein RBBP4-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_28241</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019181465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6E2"/>
    <dbReference type="STRING" id="683960.A0A1E3P6E2"/>
    <dbReference type="GeneID" id="30198711"/>
    <dbReference type="OrthoDB" id="427795at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="7">
        <location>
            <begin position="41"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="177"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="221"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="308"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="365"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="421" mass="47802" checksum="544F48394479CD63" modified="2017-01-18" version="1">MSKVTFEEAVQKAVEEKLNDLNGEEEDADDENPQKELSIQEEYQLWRKNCPVMYEFVSETALTWPSLTVQWLPDQEITSTGINQRLILGTHTSGEDTDYLKIASTQLPKNTADVNPVEQKQGVNARLKVTKKYKHESEVNRARYMPQDPNFISTISGSGKVYVYNTGLETKEAPLVLNHHTENGYGISWNRFVKGHLLTSSDDKTVALWDISKPDTPQHVLRSHTDIVNDVQWHNRDGNLFGSVSEDKTIHLYDTRSLPNPTNIIQRHAAVNTIAFSNHSSNLFAVGLDDSTIELFDIRNPQQKLHTIMGHSESITSLEWDPHNDGILASGSQDRRVILWDIKKIGEEQIQEDEDDGAPELFMMHAGHTSGITDLSFNPNIPWTLATSSDDNIVHLWKIAKKLTNEYYGEVEEDIDYFSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6N4</accession>
    <name>A0A1E3P6N4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ataxin-10 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Copper transport protein 86</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_77731</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play a role in the regulation of cytokinesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ataxin-10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040292.1">
        <property type="nucleotide sequence ID" value="XM_019184600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6N4"/>
    <dbReference type="GeneID" id="30201846"/>
    <dbReference type="OrthoDB" id="379794at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051374">
        <property type="entry name" value="Ataxin-10/CTR86_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019156">
        <property type="entry name" value="Ataxin-10_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13255">
        <property type="entry name" value="ATAXIN-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13255:SF0">
        <property type="entry name" value="ATAXIN-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09759">
        <property type="entry name" value="Atx10homo_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ataxin-10" evidence="7">
        <location>
            <begin position="375"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="479" mass="55365" checksum="E54DA46024DC52F8" modified="2017-01-18" version="1">MVDIGSSLLVLEKVLEVVGSESRSEEPLLDVMNQLTVIVKSSITDLELRHEMAHSHHIWRMIKVDLEQDPEEVNLELKFRLIRGVVLLARNLLSVARDIALEIGIRQAIFKFGLNLQKIEENEGIYNNCLVSAFQCLSNLTVNRKDHEPEVLYELVAVFKSIKIWNEDTQLPVFSYFKNIMDSPDFLHFALSKGEGKPILKELLIQFEKLDLEGELSSFGILIVSIFTKLIVHESFLKFITVYNDPDYTIRYLKVAQTIITSKESWEVFELTVILSWVYELFQKTVPQIQSYFETNSQDEPLFIYQKASSSLDILATLSKYDHSRKFLLSYEGVQNLVGLLGIIQKNVKPKKLKDDQKLEAKKNLKIEYKNFPHIKSFIIEILSSLVYQNFDVQELMRELHGLELVLSNCIIDDNEPFIKERSIVCLRFLLLNNEKNQEFVSKLEAKQAVQDDTLDEAGFEVEIVDGKVKLKQKPNYKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P706</accession>
    <name>A0A1E3P706_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutaminyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_61766</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Gln) + L-glutamine + ATP = L-glutaminyl-tRNA(Gln) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20121"/>
            <dbReference type="Rhea" id="RHEA-COMP:9662"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.18" evidence="2"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040412.1">
        <property type="nucleotide sequence ID" value="XM_019183243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P706"/>
    <dbReference type="STRING" id="683960.A0A1E3P706"/>
    <dbReference type="GeneID" id="30200489"/>
    <dbReference type="OrthoDB" id="10250478at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004819">
        <property type="term" value="F:glutamine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006425">
        <property type="term" value="P:glutaminyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00807">
        <property type="entry name" value="GlnRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000007">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2420:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000015">
        <property type="entry name" value="Glutaminyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000183">
        <property type="entry name" value="Glutaminyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1290">
        <property type="entry name" value="Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004514">
        <property type="entry name" value="Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007638">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007639">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042558">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042559">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00440">
        <property type="entry name" value="glnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF4">
        <property type="entry name" value="GLUTAMINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04558">
        <property type="entry name" value="tRNA_synt_1c_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04557">
        <property type="entry name" value="tRNA_synt_1c_R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaminyl-tRNA synthetase class Ib non-specific RNA-binding" evidence="15">
        <location>
            <begin position="5"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaminyl-tRNA synthetase class Ib non-specific RNA-binding" evidence="14">
        <location>
            <begin position="167"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="12">
        <location>
            <begin position="251"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib anti-codon binding" evidence="13">
        <location>
            <begin position="567"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA synthetases class I (E and Q) anti-codon binding" evidence="16">
        <location>
            <begin position="678"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="186"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="355"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="369"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ61205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="804" mass="92450" checksum="DD8D9CF749F5C635" modified="2017-01-18" version="1">MSSTEDLSALFNKLGLEDVKIKEILKNKKVSNSFESILKESSNIEYPLDKTKRLLLHQLAILSKGETIPNRSLITEGILNDELNTSQRVDEAFKYVKANGESSTKEELNKVSGVGIVVTEDQVKNEVRNYIQANKSEIEEKRYQFLPKILANVRTIPELKWANPSLFKPIIDEELLKLLGPKDERDVVKKEKKSKKPAANTTTSKDTKKTEEPVRSMFTEGFLGDLHKVGENPQKYPELMVEHLKACQNRVQTRFPPEPNGFLHIGHSKAILVNFGYAKHNDGVCYLRYDDTNPEAEEDVYFKSILKMVNWLGFKPWKITYSSDYFDKLYELAEQLIKNDLAYVCHDTAEEIKRSRGIKEDGTPGGERIPSKDRNRPVEESLAEFRKMRDGAYKPGEATLRMKQDLSSPNPQMWDLIAYRVLNAEHPRTGDKWKIYPTYDFTHCLVDSFENITHSLCTTEFYLSRESYEWLCDALHVYRPAQREYGRLNITGTVLSKRKIAKLVNEHYVRGWDDPRLFTLEAIKRRGVPPGAILTFINTLGVTTSTTNIQVTRFESSIRKFLEDTVPRLMLILDPIEIVIDNVDDDFELTVEIPYKPGNDKFGSRKVTFVKTIYIDRSDFSEDETDSEFFRLKPNQPVGLLKIPHNISFKNYEKDSNGAITKIHVNYDDHVVKKPKTFIQWIPKSAKHNSPVKISETRVYNQLFNSENPSSHPDGFLKDINPNSEEVFKNSIVESNFQQVVSKSPWNVVDESNKEFNVVEDKETSGPEIVRFQALRVGYFALDKDSTDDKIVLNRIVSLKESSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P718</accession>
    <name>A0A1E3P718_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_61317</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039959.1">
        <property type="nucleotide sequence ID" value="XM_019183156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P718"/>
    <dbReference type="STRING" id="683960.A0A1E3P718"/>
    <dbReference type="GeneID" id="30200402"/>
    <dbReference type="OrthoDB" id="1666796at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF111">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009027339" description="Transmembrane 9 superfamily member" evidence="7">
        <location>
            <begin position="19"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="321"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="392"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="428"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="474"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="513"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="551"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="579"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="619" mass="69982" checksum="D7E45ABD7E1FA76F" modified="2017-01-18" version="1">MFASLIFTLVAVINFTNAFYLPGVAPTSYKENEKIPLLVNHITPSVFHENKNKEDYVYSFDHYHPMLHFCQPEKLEKQPESLGSIIFGDRIYNSAFDITMLKNETCKSLCQTTYPPTDAGFVNKLIENGFSYNWLIDGLPAARRLHDKRTDSDFYGAGFELGFVDKDGKAHLDNHFDIQIEYHEREDGQFRIVGVTVEPHSWDRTEAECSAQEYQPVFVSPKDATNVVFTYDITFVPSATVWATRWDKYLHVYDPKIQWFSLINFSLIVVCLSLVMAHILVRALKNDITRYNDVNLDDEFQDESGWKLVHGDVFRAPKNPLLLSVLVGSGIQLFLMSFTTIGFALLGLLSPSNRGSLSTVMFILYALFGSVGSFISGSIYKFFGGEKWKLNMILTPVLVPGGILVTFAFLNFFLIFVKSSGAVPAGTLLAIVVIWFIISVPLSAVGSLLALKKEQLSQPVRTNQIPRQIPTQPFYLKTIIVALVAGIFPFGSISVEMYFIYSSLWFNRVFYMFGFLFFCFLLMAITTSLVTVLMTYYTLCAENYKWQWRSMFIAGGCSIYVFIHAIILSKFSLGGFTTIVLYVGYSLLISVLAFILTGSIGFIASLMFVRKIYSSVKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7I2</accession>
    <name>A0A1E3P7I2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Threonine dehydratase</fullName>
            <ecNumber evidence="12">4.3.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Threonine deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_77562</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>L-threonine = 2-oxobutanoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:22108"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.3.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from L-threonine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.19" evidence="12"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040099.1">
        <property type="nucleotide sequence ID" value="XM_019184573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7I2"/>
    <dbReference type="STRING" id="683960.A0A1E3P7I2"/>
    <dbReference type="GeneID" id="30201819"/>
    <dbReference type="OrthoDB" id="4418812at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00054"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901705">
        <property type="term" value="P:L-isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04906">
        <property type="entry name" value="ACT_ThrD-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04907">
        <property type="entry name" value="ACT_ThrD-I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01562">
        <property type="entry name" value="Thr-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1020.10:FF:000001">
        <property type="entry name" value="L-threonine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000008">
        <property type="entry name" value="L-threonine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1020.10">
        <property type="entry name" value="Biosynthetic Threonine Deaminase, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001721">
        <property type="entry name" value="TD_ACT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038110">
        <property type="entry name" value="TD_ACT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005787">
        <property type="entry name" value="Thr_deHydtase_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01124">
        <property type="entry name" value="ilvA_2Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006674">
        <property type="entry name" value="PRK09224.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF11">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00585">
        <property type="entry name" value="Thr_dehydrat_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51672">
        <property type="entry name" value="ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7 12" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ACT-like" evidence="13">
        <location>
            <begin position="470"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ60892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="551" mass="61643" checksum="84143DA5B3258CA2" modified="2017-01-18" version="1">MVLAKRFVHLHSALRSYRRYSTKYPDLLKQDLLPTGEPDYVRLILTSRVYDVVDETPVSHAVSLSHKTGSNVYLKREDLLPVFSFKLRGAYNMIAKLDPTRKSKGVIACSAGNHAQGVAFSAKNLGIPATIVMPTATPSIKYQNVSRLGAQVVLYGDDFDSAKSECTRLSEKNGLTNIPPFDHPYVIAGQGTIAMELLRQTNGEKITAVFCAVGGGGLISGIGAYLKRIAPHIKIIGVETHDADALYQSLQAKKRVQLDTVGSFADGTAVKIIGEETFRVCEEVVDEVVKVDTDEICAAIKDVFEDTRSIVEPSGAMTVAGMKRYIVDHPELDHTKSTYIPVLSGANMNFDRLRFVSERAVLGEGKEVFMLISIPDVPGSFQKLQNIIHPRTVTEFSYRYNENQETDRSTVQNAFIYTSFSVINREKEIAQVMKELEKLGFSAIDISQNEMAKSHGRYLVGGSANLPNERLLNFEFPERPGALTKFLEHLKGSWNLTLFHYRNHGSDIGKVLVGLDVPREEEEQFQQFLRDINYIYTDETDNLVYRKFLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7W2</accession>
    <name>A0A1E3P7W2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_78133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040716.1">
        <property type="nucleotide sequence ID" value="XM_019184670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7W2"/>
    <dbReference type="STRING" id="683960.A0A1E3P7W2"/>
    <dbReference type="GeneID" id="30201916"/>
    <dbReference type="OrthoDB" id="5354116at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="420"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="458" mass="52370" checksum="F44115C5BE26F659" modified="2017-01-18" version="1">MSVTKTEQNNVKNIEDCLKEIFLVLKAADKTIKVLETQIPIEFEESIPTQISESYHALVEDLGKFESNTVLDRIEKSYYKSIYSLYHDVRLSAYISLLNEEIGSPHYVDIDRFYKFATEFILRESYRLHIQISELEEADKKALRETPPSDFEETLSQDFVKISTTYSLGNSEAFFIITQNNVPLFSSLNQRSSLDEREILPPEAFSTTTVVPQAGSIPATNLGFLSPQVGRIPQPTLPPTEILSRFLHPNWYSLPTAKWLEGSDLQSFAPVIDEQNTVVTSQDKGRLWLEQIGYTKFFEKTKTEDKNEEVETSKEDVDVEMNDVTKEDGKDDDQSEEIGTISLDNLYEWAPGNDIDDDEIEAFEKGTESQFVNEILSKLQALKKKRLSESTELSYPTEEEKKLYHKANRILREIILSSGHVPDWKPSPSLPILQTNYSGTLPVPTQNINSKKKNKSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7W3</accession>
    <name>A0A1E3P7W3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ribonuclease III</fullName>
            <ecNumber evidence="2">3.1.26.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_61958</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.26.3" evidence="2"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040607.1">
        <property type="nucleotide sequence ID" value="XM_019183285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7W3"/>
    <dbReference type="STRING" id="683960.A0A1E3P7W3"/>
    <dbReference type="GeneID" id="30200531"/>
    <dbReference type="OrthoDB" id="2392202at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034963">
        <property type="term" value="P:box C/D sno(s)RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030847">
        <property type="term" value="P:termination of RNA polymerase II transcription, exosome-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19876">
        <property type="entry name" value="DSRM_RNT1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000001">
        <property type="entry name" value="Ribonuclease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044449">
        <property type="entry name" value="Rnt1/Pac1_DSRM_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207:SF0">
        <property type="entry name" value="RIBONUCLEASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="RIBONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="10">
        <location>
            <begin position="126"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="9">
        <location>
            <begin position="323"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ61400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="417" mass="46754" checksum="1C6DF50CECE2EE78" modified="2017-01-18" version="1">MSTKFDSEKIDITYGRLFQLKKDFHDLEHSFENITKFGLNLADIKQLNQSEDVPEEIKEVTNHPSIMAAANLKTAYQRGSLNNMAGLLNPSTAIETKKSNSSSSSTSSSKLDKYTIDPKGLPQLPNIENRRIYQTVFIHSSAANSTKYDVHESYERLEFLGDSVLNNVISVLLFTKFPEYQEGLLTELRSIIVCNKNLTQWSLAYGFDRNLVSNLNLLEQNMAHKKLYGDIFEAYVGGLYLDSNRELHKITPWLTRLASPIIEKFASLNPVEQVPTFIPNITSESNPVQQDLTKYDISAEVLSAKVKHCKEDLYVLLNPMFVPSYELLEKLDAEHKPTFRVACVVNGEVLGVAIAGNKKDAGSKAAYDALTNNKQKVDKYAAYKTLAKRGGPTPLVVTTPIEIQIAQGIYDTNPMPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P852</accession>
    <name>A0A1E3P852_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_75793</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040790.1">
        <property type="nucleotide sequence ID" value="XM_019184262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P852"/>
    <dbReference type="STRING" id="683960.A0A1E3P852"/>
    <dbReference type="GeneID" id="30201508"/>
    <dbReference type="OrthoDB" id="3980623at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053157">
        <property type="entry name" value="Sterol_Uptake_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784:SF5">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AFLATOXINBRP"/>
    </dbReference>
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        <property type="entry name" value="GAL4"/>
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    </dbReference>
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        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
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        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="47"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="490" mass="57221" checksum="F21BEB3D019B1174" modified="2017-01-18" version="1">MEMEMMTNSPAISKTRRNYRALVPKSSIVNVDIECMNKPKQKRSRNGCLNCKRLKIKCNENKPECEYCVQTGKECVYKVPNRVQKSRKENQISAISNEPLQKLNSVAFQLNISKLELQLLKFYIDFGGNFFSMKDFNKDSYYFWRDDIPKLWCSSDLIKNALYTISSARLLANYDFDTSKEIYIEVEDQTSIPKTPYKVNLNNQAMKYMKGTLELIDMYQRMIDDETINLEESKELLGQLLVAKKLCTGSRVILPRPRNNHKFTNIKDSYLLELMYSTNKFLIGFGKYGSMVKETQYSKVFDPELIPPRDYIEKFQNLKLGIIKHLEDYINEKAEGFDVSQISYQTAISRLENSCFQTLYFTYTMALFRGIVYMSSDIDYVHLLEQGDHIAMKIMFYTCCLNSIFHCHSYHRSGIHNEFLEFYVDYAKEKFDGGWEDDVDKNIYGWVEARARSNYGFSLNAIEHVSEPVDNYLGDDNILQISILETETSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8J4</accession>
    <name>A0A1E3P8J4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cAMP-independent regulatory protein pac2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_29124</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040479.1">
        <property type="nucleotide sequence ID" value="XM_019181579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8J4"/>
    <dbReference type="GeneID" id="30198825"/>
    <dbReference type="OrthoDB" id="5572844at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018608">
        <property type="entry name" value="Gti1/Pac2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027:SF1">
        <property type="entry name" value="CAMP INDEPENDENT REGULATORY PROTEIN (AFU_ORTHOLOGUE AFUA_3G09640)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09729">
        <property type="entry name" value="Gti1_Pac2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="9506" checksum="6B2781F69B817DB8" modified="2017-01-18" version="1" fragment="single">MESYNGAVLSPNDALILLEAARQRLIPKITRRLKDHERQLIKPGSIFIWDEKEAKMRRWTDGRSWSASRVTGAFLTYREME</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8K0</accession>
    <name>A0A1E3P8K0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA (guanine(9)-N1)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.221</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA methyltransferase 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA(m1G9)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_77842</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>guanosine(9) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43156"/>
            <dbReference type="Rhea" id="RHEA-COMP:10367"/>
            <dbReference type="Rhea" id="RHEA-COMP:10368"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.221"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.221" evidence="1"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040404.1">
        <property type="nucleotide sequence ID" value="XM_019184623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8K0"/>
    <dbReference type="STRING" id="683960.A0A1E3P8K0"/>
    <dbReference type="GeneID" id="30201869"/>
    <dbReference type="OrthoDB" id="278300at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052905">
        <property type="term" value="F:tRNA (guanosine(9)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18089">
        <property type="entry name" value="SPOUT_Trm10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028564">
        <property type="entry name" value="MT_TRM10-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038459">
        <property type="entry name" value="MT_TRM10-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016653">
        <property type="entry name" value="TRM10/TRM10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007356">
        <property type="entry name" value="tRNA_m1G_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563">
        <property type="entry name" value="TRNA (GUANINE-9-) METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563:SF13">
        <property type="entry name" value="TRNA METHYLTRANSFERASE 10 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016323">
        <property type="entry name" value="tRNA_m1G_mtfrase_met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51675">
        <property type="entry name" value="SAM_MT_TRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase TRM10-type" evidence="12">
        <location>
            <begin position="92"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="283"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="307"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="335"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR016323-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR016323-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ61197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="344" mass="39284" checksum="7B28C77FBDF2ECD5" modified="2017-01-18" version="1">MSDQPGTAQSPVQDAPKAADYPTLESIPEVKPAEMSKSQWKKLQRKKKFQENKKEYIKIRKEKKKENNAARRLEIQSYVEKNEPIPEHLLKKPKKPKTQTKTGSKIVIDCSFDHLMNDKEKVSLSSQITRAYAANRTSPNTVDLEVTSFGGNLKRRFEVDMKSSAYQNWKNITFNEEPFEPSENVIYLSADAEEALEVLEPDMTYVIGGIVDKGRYKFLCKNKADELGLKCLRLPIDEYINLFGRRVLTTTHVVELMLEWWNGKDWKLAFEKVLPQRKLINEDKEGSTSVDPEEIASVDPEKIAKLEDDEEDEEEAEAEEVAPESEKKPEPVQTADATEGTSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8P9</accession>
    <name>A0A1E3P8P9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_67844</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040454.1">
        <property type="nucleotide sequence ID" value="XM_019184101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8P9"/>
    <dbReference type="STRING" id="683960.A0A1E3P8P9"/>
    <dbReference type="GeneID" id="30201347"/>
    <dbReference type="OrthoDB" id="3437016at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF191">
        <property type="entry name" value="VACUOLAR BASIC AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="84"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="339"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="385"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="448"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="478"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="556"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="80"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ61247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="586" mass="63330" checksum="B6459D9AEF2C51EC" modified="2017-01-18" version="1">MSNSDSPLLKPYSKALDDPTLGSPGLVARELAQPDENIDSLTIGNGNTYGAIDQDLESQPQETEQDPNVGEIDPAQLKLIIGTLYIGSFLAALDTTIVTTLLSTIASSINSVSKMSWIATSYLLSCSAFQPLYGKLSDIYGRKSLLLFSNATFALGCLISGLSHNLVGLSIGRFITGIGGGGLTSLSTITTSDLVPLRKRGIYQGLGNIAFGVGAATGGIYGALFEKYLGWEYAFFSQVPIALISVYLIWRNLKLPEGSIGLGMTGSRLAKLKQVDFLGATLLVSALLSFMILVSFAGNEIEINGGAFWGLIVFTAVTLGSFGYVELYVSPQPVLPVRLFAFITVLSSGLVNWFMTMSVFTYLFYMPVYWSSVVGLTPTEIGLRTISNFVGVSMGSVLSGIYMKNTGKYYWFSVIAGILTILGVYGLYVTTETTSTFFQYIELFIPGAGYAASLTVTLLALIASVPHDQQAATTSIQYAFRATGSTIGVAASSFIFQKYLLSSLTTRLFSIPSDYSKEEVLEIIQKAFNSSEYTRKAPEVFRDAIIKSYDIGTHKALLFSFITCVIACVVGLFMKEHVLHTTIHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8R8</accession>
    <name>A0A1E3P8R8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">VPS37 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_59883</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS37 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041012.1">
        <property type="nucleotide sequence ID" value="XM_019182896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8R8"/>
    <dbReference type="STRING" id="683960.A0A1E3P8R8"/>
    <dbReference type="GeneID" id="30200142"/>
    <dbReference type="OrthoDB" id="10260857at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009851">
        <property type="entry name" value="Mod_r"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07200">
        <property type="entry name" value="Mod_r"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VPS37 C-terminal" evidence="8">
        <location>
            <begin position="43"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="164" mass="18249" checksum="01A3228705EC1A60" modified="2017-01-18" version="1">MSTPPSLPPKTPEYGTSNAQQDASPQPTPQPIARIEVSEDIRSLPTDQLLRLMESIDILKGYLIQLSEKELNQSASELDTIIGDIDHFIKSLSQLQTSKDALNKQLSELQRLVEEWESKEVAMYASLKKFGPENIYSLLSSSNTMSLQFKVSSSLIEMNGNCTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8W7</accession>
    <name>A0A1E3P8W7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_29489</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040599.1">
        <property type="nucleotide sequence ID" value="XM_019181614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8W7"/>
    <dbReference type="GeneID" id="30198860"/>
    <dbReference type="OrthoDB" id="3975247at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032218">
        <property type="term" value="P:riboflavin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF177">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER MCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="342"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="377"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="405"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="471"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="502"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="112"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="532" mass="58148" checksum="A32245ACA38D410B" modified="2017-01-18" version="1">MIDGLKITSSPIAFPRTAAVKNEGSKKDFYELQDQTTGVRIGKKNESVEETYELQDQTGVPLPIEGASGSGFAWHQSSGTPIDQRSPVEELNEDEIFLLDDDVESFPEGGLSAYLVVFGSFMGLIPVFGLVNSAGAIEAYVSSHQLAQVSTSTVSWIFSINVFIAFSSGIFSGAFFDRNGSRPPMIIGAVFFSAGLFATGNAQSVYQFVLAFGVVNGFGLGMLMSPLVGVVAHYFKKKRATYTSIATTGGSIGGIIMPLLLRSLYPKVGFAWAMRILSFICLFCFACAITFAKERISNKPQADHEIEGEDKSRLTAKEFIKIYILDSYFNSFDFKALKDPKYFFCVLGAVFAEISAISSITYFASYSKTRGFSVNDSFILVSLVNAGTLPGRLITGYLADKVGRFNVFIVTIILTAVVNLTVWMPFGYNSKVLYAYSVFYGVFSGSVFSLLPVCCGQVCKTEDFGKRYSTMYFIIAFGTLFGVPIGGAIIGDKSVERYNYFIIYTAVTAVASASCYVISRYYCIGKKVFARF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8X3</accession>
    <name>A0A1E3P8X3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_86839</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041070.1">
        <property type="nucleotide sequence ID" value="XM_019186073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8X3"/>
    <dbReference type="STRING" id="683960.A0A1E3P8X3"/>
    <dbReference type="GeneID" id="30203319"/>
    <dbReference type="OrthoDB" id="73901at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF27">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN CTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0186">Copper</keyword>
    <keyword evidence="5" id="KW-0187">Copper transport</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="234" mass="25646" checksum="B5009D858D499262" modified="2017-01-18" version="1">MAMSSSTPTASSSMNMGGMGGMGHHTTASMSMPMSSSTGSSSMDMGMHMSMNTFLTTKYSGYPVVFKNLSAASGGAAFGIFCVLFFTAFAFRGLGFLSAYLEQTVFRDPKKFDLSENETTHHTHQDEKNVINESTRSTDMESVLVKKRDAENNRSVIAKFFAVTPSSLYRDFIRVILAFVSAMLGYALMLAAMSFIIPYFFAIILGLSFGEVFFHRLASVMEINTNNSICESLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9K7</accession>
    <name>A0A1E3P9K7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Pre-rRNA-processing protein IPI3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_38097</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the RIX1 complex, composed of IPI1, RIX1/IPI2 and IPI3 in a 1:2:2 stoichiometry. The complex interacts (via RIX1) with MDN1 (via its hexameric AAA ATPase ring) and the pre-60S ribosome particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the WD repeat IPI3/WDR18 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041302.1">
        <property type="nucleotide sequence ID" value="XM_019182532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9K7"/>
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    </dbReference>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="EnsemblFungi"/>
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    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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        <property type="entry name" value="WDR18/Ipi3/RID3"/>
    </dbReference>
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        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 6 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="145"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002355"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010143"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ62095.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="484" mass="53881" checksum="7A3A3A15A066ED13" modified="2017-01-18" version="1">MEEVVYYNCEGFGDDKNSAQVNGIATSIHSQSHNLAFRQSSSYPHGSAITGTNPGDRVFVASKGKALISVYTWGKESPDQRIPVPEQLTALTLCPNSQELYTENFESEDESNIPKFRLPYLLIGGGVSGMIYTWELNSGLLLSVKEAHYQSISALKTTNDGSFLVSAGKDARILIWKITDLVSFVKDDDRVIKPVHVISDNTLEITDIFINNSIYQDTKLYTVSRDCTIRIYDIVKFQLLSTFIIGQQIESIVVDSADRAIYIGLSNGNIRQINIYEPNPATNILEARGGFGKVVTLSEDHQLTNTLTHHSPNAVTSLALSLDGSLIISGDSIGKVTVADVVSKQVVKELKELGSKITNIQVINFYKSNQIVEKTQKAIPQFKRIISNKNPKEQEVVYQIGEDDEKKLFDIDAHLDRVAQEALQFENLTTINSEVVMTDAYDGSSSEKVQELEGKVTKATKAYTDLRKMYEDLYSEHTKLLENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9R2</accession>
    <name>A0A1E3P9R2_WICAA</name>
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        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_60219</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
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    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="ODQ62153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Ribonucl_Kappa"/>
    </dbReference>
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        <property type="entry name" value="RNase_K"/>
    </dbReference>
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        <property type="entry name" value="RIBONUCLEASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="84" mass="9182" checksum="CA995CAE275DD79B" modified="2017-01-18" version="1">MKAVVSPGKAWFCTVLSAFGVVILSFIGYLFSINHESMMGSVNDPEDGKAVAHTVFIAALVYLGFFVFCGSQILVYKRQSRISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9R7</accession>
    <name>A0A1E3P9R7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carboxymuconolactone decarboxylase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_26670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041363.1">
        <property type="nucleotide sequence ID" value="XM_019181288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9R7"/>
    <dbReference type="STRING" id="683960.A0A1E3P9R7"/>
    <dbReference type="GeneID" id="30198534"/>
    <dbReference type="OrthoDB" id="5537330at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052999">
        <property type="entry name" value="PTS1_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28180">
        <property type="entry name" value="CONSERVED MITOCHONDRIAL PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28180:SF2">
        <property type="entry name" value="PEROXISOMAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxymuconolactone decarboxylase-like" evidence="1">
        <location>
            <begin position="157"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="225" mass="25533" checksum="7689AD8F9D7FAB25" modified="2017-01-18" version="1">MVLTPQRLVQLSKYPLLKNSWYFIAAATFSVCNQPNEVARLVHYALKQSQYESSNKSIIDPTRESILKSLALGGIPKAINTMMYLKNATPVSLQDSKPNRDFDSSKTDQVRERGIEFWHHVYGKVSNRIINQMNTAYPDLWEYAKNHIYSPLLSYSDILTPKETSLVIIGCLVPQDVNPQLKGHLKGALNNGATPEEVEQARQLALSISEWCDIKWKEDVAKLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9T3</accession>
    <name>A0A1E3P9T3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_76354</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
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            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="UNCHARACTERIZED NODULIN-LIKE PROTEIN"/>
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            <end position="36"/>
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        <location>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ62176.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="524" mass="57070" checksum="43311713F67B7243" modified="2017-01-18" version="1">MSESQQFFEYPPVGARIVSLVASVIVATASGTPYVYSVFAPQLVKNSKLPVSSLSNLSLALALGGACGGFPAGLMIDKFGPGPADFIGSIFQFTAFTLLHKIYTTQSQNIPLLMVALACVGFGSILSFYSTVKCATANFPHHRGTAGALPVSAYALAALLYSSIAVNFFKDNTAGLLKFFSLFCPTLCVASSFFLRIYKKEKRSDATVSANSESAIAEERTTMKTVPSAASLMSAFNERRRSLRGSLSFWGIGVRSPRGSVSSFSQENRPLLAPELRRADSSVIYSAVEDVPVFVREHSPIWNHYIVKAICQRLFLKYYFILASLQGIGQMYIYSVGFIVVAQVNSTPNKKLTVGQSQALQVSTIAIASFLGRLTSGPISDVFRRKFHAQRIWCIFFASSLMGLGQHLTSIFDSVEQITLCSFITGFAFGFVFGTFPAVVADRFGTEGFSTIWGLMTTGGIITVSQLTKLFGYYLGKHTDEDGVCRAGLECYREAFVFTKWASLFISALLLLTIYINHRLGTKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA35</accession>
    <name>A0A1E3PA35_WICAA</name>
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        <recommendedName>
            <fullName evidence="9">Ribosomal RNA-processing protein 14/surfeit locus protein 6 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_60343</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
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    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
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            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SURF6 family.</text>
    </comment>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029190">
        <property type="entry name" value="Rrp14/SURF6_C"/>
    </dbReference>
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        <property type="entry name" value="Rrp14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007019">
        <property type="entry name" value="SURF6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14369">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14369:SF0">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15459">
        <property type="entry name" value="RRP14"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SURF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="8"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA-processing protein 14/surfeit locus protein 6 C-terminal" evidence="5">
        <location>
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            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="35"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="371"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
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        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
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            <end position="141"/>
        </location>
    </feature>
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        <location>
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            <end position="169"/>
        </location>
    </feature>
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            <end position="205"/>
        </location>
    </feature>
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            <end position="229"/>
        </location>
    </feature>
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            <end position="244"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        <location>
            <begin position="436"/>
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        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04935"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF15459"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ62276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="445" mass="51507" checksum="E6B02E00E85639D7" modified="2017-01-18" version="1">MSSTLEERLKNNSSAFDGLLSLIPAKYYYDDDTQNQWKAKKQSKEEAKEAKRAKLDPVQSEQSDQSASASQVLKKRAKTAKPVVIPGSKLIQKPIVEEENDDEEEEEEIEEESNEEEAKPVKQTNQKKDQKKEVKPKDKSQKVNGFDEEDEEEENINIMFDDEGNEVDFEQPKPKDESTGNSVEVKEEEKKKKDESIKLLREKLASKINALKEKRKAPGSKAAGAPSSREAILEERRKKAEQKAAQKKRKLEELEQDDDDNSDEDEESDDGEEDDGMSADNVLYQNIRFNDDERTTSDLQNLRKHSKKKGPAKKDLKAHLKLVEAKKQKLAQLDQFELKEQNEKDKWNTVIAQAEGEKLRNDEKLLRKALKRKDAQKRKSEFEWKERKETVENSISAKQKRREENLQIRKDNKGIKRKNQAKQKRKFKGAIVPKRAGFEGRRSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAL6</accession>
    <name>A0A1E3PAL6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH-ubiquinone oxidoreductase ESSS subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_60524</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041673.1">
        <property type="nucleotide sequence ID" value="XM_019183011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAL6"/>
    <dbReference type="STRING" id="683960.A0A1E3PAL6"/>
    <dbReference type="GeneID" id="30200257"/>
    <dbReference type="OrthoDB" id="2147978at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="243" mass="27893" checksum="CC51DC45D018366E" modified="2017-01-18" version="1">MMLRSILKKSVITRGFHSTRTASAFKKIAEEDIRYLIDSLPPASKLYQNPDGSPREPTDLELHKLAHLSVLNEKRKLTLWDYMFLSEDNAKLYADNYTDIQNLLPNSENGASGNIIDKIPYEDAEGNVKWRVVREVEKEGWEDLTRWGLIPAYLLFVAVYLFKDDKGINEWAYDELKLRALEDLEGTDAAKETLNNENKSPEEIKQRDELIVERILSGEYDKLAGLKINAQNLSMIGDLNTQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAN9</accession>
    <name>A0A1E3PAN9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Actin cytoskeleton-regulatory complex protein END3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Actin cytoskeleton-regulatory complex protein end3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Endocytosis protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">WICANDRAFT_38719</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Actin patch</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the END3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041635.1">
        <property type="nucleotide sequence ID" value="XM_019182538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAN9"/>
    <dbReference type="STRING" id="683960.A0A1E3PAN9"/>
    <dbReference type="GeneID" id="30199784"/>
    <dbReference type="OrthoDB" id="1716625at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990964">
        <property type="term" value="C:actin cytoskeleton-regulatory complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000323">
        <property type="entry name" value="Actin cytoskeleton-regulatory complex protein end3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025604">
        <property type="entry name" value="End3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12761">
        <property type="entry name" value="End3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EH" evidence="21">
        <location>
            <begin position="9"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="22">
        <location>
            <begin position="41"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="21">
        <location>
            <begin position="139"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="360"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="298"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="311"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="ODQ62428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="395" mass="44905" checksum="DA6C74244A7865CB" modified="2017-01-18" version="1">MAPKLEEWEIKKYWEIFSGLKPVDNKLTGDQVSVVLKNSKLSDSNLSTIWDLSDIDSDGSLDFEEFCICMRLIFDLVNGTISSLPSQLPDWLVPSSKAHLIQANRALSGQDQQQLRYSDSEDEESSLSDQFDWYISPNDKSRYEEIYSSASDRFGRITYDSLNGLYNTLPNVPATDISSAWNLVNPRSSETIDKDQTLVFLHMLNQRSTGKRIPRSVPSSLRATFSKETPEYSLDSHQADIHKSGNSKHSFADNYLERIGGSELSKNVKGTDFSSTEGTDWEEVRLKRELADLEEKVRQKELETDSKTNERNNVLNGNGTTSNKTLQIRKELEQLLEYKQKQLVQDSKTGVDLSEISNDIDTVESQVQVLEQYLQQKKNEYNELKNQIAQAKSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB20</accession>
    <name>A0A1E3PB20_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">V-type proton ATPase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_27122</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments. This subunit is a non-integral membrane component of the membrane pore domain and is required for proper assembly of the V0 sector. Might be involved in the regulated assembly of V1 subunits onto the membrane sector or alternatively may prevent the passage of protons through V0 pores.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the V-ATPase V0D/AC39 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041823.1">
        <property type="nucleotide sequence ID" value="XM_019181338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB20"/>
    <dbReference type="STRING" id="683960.A0A1E3PB20"/>
    <dbReference type="GeneID" id="30198584"/>
    <dbReference type="OrthoDB" id="10250083at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1690.10:FF:000003">
        <property type="entry name" value="V-type proton ATPase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1690.10:FF:000005">
        <property type="entry name" value="V-type proton ATPase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.50">
        <property type="entry name" value="ATP synthase (C/AC39) subunit, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1690.10">
        <property type="entry name" value="V-type ATP synthase subunit C domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036079">
        <property type="entry name" value="ATPase_csu/dsu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002843">
        <property type="entry name" value="ATPase_V0-cplx_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016727">
        <property type="entry name" value="ATPase_V0-cplx_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035067">
        <property type="entry name" value="V-type_ATPase_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044911">
        <property type="entry name" value="V-type_ATPase_csu/dsu_dom_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11028">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01992">
        <property type="entry name" value="vATP-synt_AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018497">
        <property type="entry name" value="V-ATP_synth_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103486">
        <property type="entry name" value="V-type ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR018497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="344" mass="39700" checksum="BFC09130EBADE018" modified="2017-01-18" version="1">MEGLYFNIDNGYIEGVVRGYRNGLLNSNQYINLTQCDTLEDLKLQLSSTDYGNFLANVPSPLATSTIQDNAAQKLYSEFNYIRDQAVQPLSKFMDYITYGYMIDNVALMITGTVHDRDKAEILDRCHPLGWFDTLPTLTVATDIDSLYQTVLVDTPLAPYFKDCLTAEDLDDLNIEIIRNKLYKAYLEDFYKFCLEQPDPASTIMKTLLEFEADRRSINISLNSFDSELSPEEKKSLLPAFGKLYPMGTEALARADDVEQIRSIVENVYEYKSFFDNVGGKTLEDHFYKLEMELCRDAFTQQFTISTIWAWMKSKEQEIRNITWIAECIAQNQKDRINNYISVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB86</accession>
    <name>A0A1E3PB86_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_88393</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041858.1">
        <property type="nucleotide sequence ID" value="XM_019186150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB86"/>
    <dbReference type="STRING" id="683960.A0A1E3PB86"/>
    <dbReference type="GeneID" id="30203396"/>
    <dbReference type="OrthoDB" id="296767at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013887">
        <property type="entry name" value="UPF0592"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37988">
        <property type="entry name" value="UPF0592 MEMBRANE PROTEIN C7D4.03C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37988:SF1">
        <property type="entry name" value="UPF0592 MEMBRANE PROTEIN C7D4.03C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08578">
        <property type="entry name" value="DUF1765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="503"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="558"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="503"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="521"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="569"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="602"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="645"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="866" mass="98500" checksum="D423C8EBA7B750F9" modified="2017-01-18" version="1">MKTISLSINAFIGKVFAYSFLNLDKFGHMLLFLLFVKNYTIKQILTHNDIQKLIPNNVSFKKINPSLANLVNFKGDFTEYLFIESPNQTKLQSLNCMKPPKTAYLDDLFIDLKDNNWVKKWAGFDSDIFCSFLKHYLSLSIDTLDASVLDFKELFKLPGFIIIYGHITEILESNIMMIKKFKSTQSKDTNYNSNSSTYNALKNFNIFLQKLPIIKIMRVIRDLVYEGDERKSSMVISMFENILYSKAKCVQAFNSVTCEAIYTIFLEFLLHLQNDQYCFTRNLNLSFWITGLLKMLGTDNMICQARALSHLFNIWNLIPNDSFINQKNIFGYDPSEWVKNPKNSLLFNLSNHLLSQSVWEAFFNHWHPLIRQFYQRLLVFKIIGNSTDNYSNYLNFKKVLKSNLVTTFEIFSEKTADLNISPILNMDLTPANPLLNRKLIISPMESFQSSINILDNFDSYQFLQNGAYSSRNSSSSTLIIDSNKKIYAYDIFDEAIYSSSIGSTSNNNPRTSVSNSSTRKNTPNSSPSVTRSSSATTLGRVPILGSALSFFKKVNHSQPTIKVSPNPSPLSSPQALSSPYQGPEDQDPLKIRQSTTLHPVASISSSSSRSPSFKTPTSLLSSPSSSLSEISDEDQFNSSRKNRQVSSNTLNSSTSSRLSSTSSTSSVSTSTTPMESLPTPPELLVKVPEVKRFKFRFQLTISNDPSQLSHRIVNASKTSSKKFFPSEGHKAQLPKFPRLPFDNKSNELGLMNNSSSFYRFEQADESFLNSSDEDDEFLVIPRSSMSSRVQEEESKITFESTKNFLLLGKVLNEWELIVDEYEKFIKLTNEMDELNHEVKDYEEFFHDPMLVADIPPTKVNGFNAHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB89</accession>
    <name>A0A1E3PB89_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_60613</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041767.1">
        <property type="nucleotide sequence ID" value="XM_019183025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB89"/>
    <dbReference type="STRING" id="683960.A0A1E3PB89"/>
    <dbReference type="GeneID" id="30200271"/>
    <dbReference type="OrthoDB" id="2102561at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000140">
        <property type="term" value="F:acylglycerone-phosphate reductase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169:SF6">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="289" mass="32914" checksum="2BB003639D509560" modified="2017-01-18" version="1">MSKVALITGTNSGIGYSLALEFHKRGVKVYATDYQFSNTTLQTFKDLGIITHTLDVRNIQDIKKIRDVVDAEENGKLDYLYNNAGIVTAVHSTDITDEQLQDLYDINVFGPIKMTREFIKLVINAKGTIIFSGSVTKNIPLHCNSLYVSSKAALDQYISVLQLEVRNYGVKVIEVLGGMIKTDIFNNSMNTVPDDSIYNFDEYTEIFQNRKQALDKNNHKRMPADIFAKRVLDQVEKSGLNTFRIFEGGEASRLYYIPNFLPRSKILDIMLGMFNLNFNYRRHLPAEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB97</accession>
    <name>A0A1E3PB97_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">40S ribosomal protein S29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_82643</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS14 family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ62661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019185415.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3PB97"/>
    <dbReference type="GeneID" id="30202661"/>
    <dbReference type="OrthoDB" id="10252683at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="40S ribosomal protein S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.10">
        <property type="entry name" value="30s Ribosomal Protein S14, Chain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_uS14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018271">
        <property type="entry name" value="Ribosomal_uS14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039744">
        <property type="entry name" value="RIbosomal_uS14_euk_arc"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_uS14_sf"/>
    </dbReference>
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        <property type="entry name" value="PRK05766.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="40S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RIBOSOMAL_S14"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="56" mass="6668" checksum="F5F6BF4B175BC052" modified="2017-01-18" version="1">MAHENVWFSHPRKYGKGSRQCRICSSHSGLIRKYGLDMCRQCFREKAKDVGFNKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBH6</accession>
    <name>A0A1E3PBH6_WICAA</name>
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        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="8" type="ORF">WICANDRAFT_26951</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041857.1">
        <property type="nucleotide sequence ID" value="XM_019181320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBH6"/>
    <dbReference type="STRING" id="683960.A0A1E3PBH6"/>
    <dbReference type="GeneID" id="30198566"/>
    <dbReference type="OrthoDB" id="3269380at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001987">
        <property type="entry name" value="Transcription factor SFP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051580">
        <property type="entry name" value="ZnF-Chromatin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23057">
        <property type="entry name" value="JUXTAPOSED WITH ANOTHER ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23057:SF0">
        <property type="entry name" value="JUXTAPOSED WITH ANOTHER ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="283"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="338"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="190"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="362" mass="41031" checksum="4103B512B955CB57" modified="2017-01-18" version="1">MNTNSFFNQAAAISIPNGNQGSSLSQPIDIAYQRSGSVFNNNSNFPTSNQRDSIAHSQGVGGISWGSVTIGSWLKDEVMMHNNFNLGSTNAQISMSPPIQSAYLPDLEANYCKDYSCCGQLLPTLHDLLKHYEESHISPTPPTHQPKPIQNFDTVSTTDVFINSNNNNNNMNNVNQKQQNTFGFNQSPFQQQNIQQQLRQQLQGSNNNSSSSIHQQQQHHSPHPQQQQNHHIQQQSQQQQQQQQQNYQLHQNLNNPQQVNMDEGYIDDPSRNLYVAQGNEKPFTCPVIGCNKTYKNQNGLKYHRLHGHQNQKLHLNPDGTYSVIDPNDGNGMEQDKPYRCEVCGKRYKNLNGLKYHRGHSTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBI0</accession>
    <name>A0A1E3PBI0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase small domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_76743</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041781.1">
        <property type="nucleotide sequence ID" value="XM_019184444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBI0"/>
    <dbReference type="STRING" id="683960.A0A1E3PBI0"/>
    <dbReference type="GeneID" id="30201690"/>
    <dbReference type="OrthoDB" id="194386at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF132">
        <property type="entry name" value="PROTEIN-LYSINE METHYLTRANSFERASE C42C1.13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="43818" checksum="3186044008DED960" modified="2017-01-18" version="1">MYQIKFLKPLTLNQGSLLPKNPKKNQRNVAASYEISSAITISNDLGEQFFYGRATLACQLRLYDSDEMKNVETKYTPIAITTVEWTAGKRAMDVKLEIPSKYLQPGKYIALNVSVDSTKQAVDEMNVSDITHDELREEWFPEWKKIVGDCVESIDISSTNLQIFPTTSSLLKISNEVPVKSERFAIRSISDVVLYEELSGEIARHLWDAGIIVNNLSKQQFEKFLYRNETSPKGTNLQILELGTGIGLVAIHLSKMSRDSEFLATDLPDAKEICTMNIGLNEVQEQVKFSELDWEDDVPALKQWDIIITTDCTYNPLYYDALIKVLKRESNKDTKIILAHKFREPITESEFFVKIQAHFHLERQMWYNAQGQSLTHIGLYSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBP4</accession>
    <name>A0A1E3PBP4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA damage response protein kinase DUN1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_82427</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041589.1">
        <property type="nucleotide sequence ID" value="XM_019185377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBP4"/>
    <dbReference type="STRING" id="683960.A0A1E3PBP4"/>
    <dbReference type="GeneID" id="30202623"/>
    <dbReference type="OrthoDB" id="407410at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FHA" evidence="6">
        <location>
            <begin position="18"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="127"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="448" mass="50569" checksum="40572E5495CCCB5C" modified="2017-01-18" version="1">MSVLPNSNKRVLPNKSTVVVGRSRSSDIVLRGSDISTRHCELIPIWNDVLQNFFLNLIDHSSNGTFVNGSRVENKNCILKNGDKLNFAKSGAYVFRYKRDEESSDEDKENVDTTSNVKPAKSFFDEYVLGRQLGSGHYATVKEAIRKETGDSFAVKIFKPQRADDTKSSKQFNQELEVLMSIKHENIVKLIGTYTEPINKFSFTTYLVLEKVNDGELFTRIVKKQNLRQDETKAIFKQLLNGLLYLHNRNIIHRDIKPENILLNIVPRTSPSQKQTGPWDENELDVSVKIADFGLAKFIGELKFTNTLCGTPAYVAPEVLNSSRKYSKNIDLWSAGVLLYVCLVGFPPFSDELGPPNMREQIISAKYAFYSPYFDAIDDLALDLITKLLVLDPDQRLNIQQAIDHPWFNDDSSAPLDTVTRSPIKGSSLPKTYTELSQMQSMEGPGVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBQ7</accession>
    <name>A0A1E3PBQ7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribonuclease</fullName>
            <ecNumber evidence="9">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_26878</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase HII family. Eukaryotic subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="9"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042015.1">
        <property type="nucleotide sequence ID" value="XM_019181311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBQ7"/>
    <dbReference type="STRING" id="683960.A0A1E3PBQ7"/>
    <dbReference type="GeneID" id="30198557"/>
    <dbReference type="OrthoDB" id="7462577at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990516">
        <property type="term" value="P:ribonucleotide excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07181">
        <property type="entry name" value="RNase_HII_eukaryota_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.460:FF:000001">
        <property type="entry name" value="Ribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000016">
        <property type="entry name" value="Ribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.460">
        <property type="entry name" value="Ribonuclease hii. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004649">
        <property type="entry name" value="RNase_H2_suA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023160">
        <property type="entry name" value="RNase_HII_hlx-loop-hlx_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00729">
        <property type="entry name" value="ribonuclease HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954:SF7">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51975">
        <property type="entry name" value="RNASE_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-2" evidence="10">
        <location>
            <begin position="66"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="343" mass="37880" checksum="24482C927507DE4C" modified="2017-01-18" version="1">MSAAAAIEEPLDIEEEDPIVTDTQETVVIPETADFLPPSVPIKHTHKSTTYLTEIPKEILASPSEPCVLGVDEAGRGPVLGPMVYGIAYCLKSYEQTLKKKYGFADSKTLTEVKRTNLLEQICLPNGELFENVGWSTRTMTAMDISSGMLRPHTVGNYNLNEQAHDATMDLIQGVLDQGVNITELYVDTVGPPVTYQAKLQKRFPNLKITVAKKADALYPCVSAASICAKVTRDFSLSMSKDELYGSETIWGSGYPSDPKTSKWLNSAVDPIFGWDQVVRFSWQTSKDAIIKNGGVDIEWEEDNIKEDYGVDVGAMFKNSKDSDLSNLPVSKLWFGKNVDCFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBZ2</accession>
    <name>A0A1E3PBZ2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin-activating enzyme E1 1</fullName>
            <ecNumber evidence="4">6.2.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_25659</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.45" evidence="4"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042098.1">
        <property type="nucleotide sequence ID" value="XM_019181171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBZ2"/>
    <dbReference type="STRING" id="683960.A0A1E3PBZ2"/>
    <dbReference type="GeneID" id="30198417"/>
    <dbReference type="OrthoDB" id="10252231at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004839">
        <property type="term" value="F:ubiquitin activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01491">
        <property type="entry name" value="Ube1_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01490">
        <property type="entry name" value="Ube1_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2660:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12550:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000015">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.180:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.60:FF:000002">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.180">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, FCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.80">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12550">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2660">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, SCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.60">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, UFD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032420">
        <property type="entry name" value="E1_4HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032418">
        <property type="entry name" value="E1_FCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042302">
        <property type="entry name" value="E1_FCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018965">
        <property type="entry name" value="Ub-activating_enz_E1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042449">
        <property type="entry name" value="Ub-E1_IAD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038252">
        <property type="entry name" value="UBA_E1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019572">
        <property type="entry name" value="UBA_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042063">
        <property type="entry name" value="Ubi_acti_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018075">
        <property type="entry name" value="UBQ-activ_enz_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000011">
        <property type="entry name" value="UBQ/SUMO-activ_enz_E1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01408">
        <property type="entry name" value="Ube1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF4">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16191">
        <property type="entry name" value="E1_4HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16190">
        <property type="entry name" value="E1_FCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09358">
        <property type="entry name" value="E1_UFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10585">
        <property type="entry name" value="UBA_E1_SCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01849">
        <property type="entry name" value="UBIQUITINACT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00985">
        <property type="entry name" value="UBA_e1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-activating enzyme E1 C-terminal" evidence="12">
        <location>
            <begin position="882"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10">
        <location>
            <position position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ62891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1012" mass="112868" checksum="38FE6A9DC5D562C5" modified="2017-01-18" version="1">MQIDTPSPQAPEIDESLYSRQLYVLGKEAMLKMQNSNILIIGLRGLGLEIAKNVALAGVKSLSIYDPNPVELKDLSTQFFLTEDDIGKPRGQASLAKLAELNSYVPISIVDELSEETLSKFQVVVLTDVSLEEQVKINNFTHSNNIKFITTDTRGLFGNVFVDFGQDFTIVDPTGEEPLTGIVSDIESDGTVSVLDDNRHGLEDGDFVKFSEVQGLESLNDGQVFKVTVPGPYAFNIGDVSKLETYKKGGIYTQVKVPQKVSYQSLAEQLETPEFVFSDFAKFDRPAQLHLGFQALHQFQTRHNSLPKPFNQEDVNEFLHLVKNLATQQKFEGDLDEKLLTELSFQAQGDIPAINAFFGGLVAQEVLKASSGKFVPIKQFLYFDSLESLPNNYPRTEENTKPIGSRYDSQIAVFGLDFQKAIANVKTFLVGSGAIGCEVLKNWALMGLGSGPEGKIIVTDNDTIEKSNLNRQFLFRPKDVGKNKSEVAALAAVAMNKGLEGKIEAKGDKVGTETENIFDNSFWESLTFVTNALDNIDARTYVDRRCVFFNKPLLESGTLGTKGNTQVVIPRLTESYSSSQDPPEKAIPLCTLRSFPNKIDHTIAWAKSLFQGYFTEAAENVNLYLSQPNFLEATLKQSGDVKGILQTISESLNNRPYSFEDCIKWARLEFEKKYNHDIKQLLFNFPEDAKTSTGAPFWSGAKRAPKPLVFDINNPDHFHFVVGAANLRAFNYGLKGDQGEPDKSFYGKIIDEVKIEPFSPRSDVKIQANDDEPDPNAQQAVETDELHQLAASLPQPSTLAGYRLNPVEFEKDDDTNHHIEFITAASNARALNYQIDVADRQKTKFIAGRIIPAIATTTALVTGLAQLELYKVVDKKDKIEDYKNGFVNLALPFFGFSEPIASPKGKYNGKEFDRIWDRFDIKGDITLQQLLDHFKEKEGLEITMLSYDVSLLYASFFPPKKVKERSQMKITELIKTVTKKDIPGHVKTLILEVCTDDAEGEDVEVPYVTITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC79</accession>
    <name>A0A1E3PC79_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_60956</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042117.1">
        <property type="nucleotide sequence ID" value="XM_019183086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC79"/>
    <dbReference type="STRING" id="683960.A0A1E3PC79"/>
    <dbReference type="GeneID" id="30200332"/>
    <dbReference type="OrthoDB" id="4091299at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="348"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="468"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="586"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="623" mass="68353" checksum="8542EC76FA625467" modified="2017-01-18" version="1">MAQFDDPSIISLVSAVSDSKAGASAFENNKDDNAITKFLREAEYKREEEEAKKSGFTTEQKGTEIGDDESTNAQSKELELEDWKLELGPYNNNERLRYSMDYLYSLKDSSDNVVPQDLPPKSYYRLKNKPIQRTNWSGNQYNNNNGNYTGRNNYNEGGPATGSMGNFNLNKKPHDRGFNRYGNNNNNNRRNNRDHRHKKQDDDDSWLAELEKDEPAGGSAEDFEKWKAKIKLAERKRRGEIVDEEEITPSSTQRSEENKNTIDSFFSVAKPSTKSGGSSNAGTPDVTKNSKFFSFFKPESGPAKDEQSSGQQPSGNDGVSKILSLLDSGEKNAKAGSNNSTPLLTKASPLQSPQVQSQQPQQGRTQFNFQQPSSGQPQPPQQPSSSAPVLPPGLAPPSNQGLPAGGSKDSFFMSLLNRGPESAKIDSTPNAQSPKVSTNDSKQSPSLPNQEQPRSSLPPGLQGPPGLPQQNQQQHSQQPGLQGQPMIPPQFQNGQNRGQGQMPPVPPGFNPQQLPPWLAQQFPPNLPQGGRFMPPPPPNGMGFPPQMRQHGPPGASNIPPHLLYPQGGQQGGQSNPQVFYPPFGAPNPDGQHQQQQQQQQRFPPGFFNGPPPPGFQGRDSNQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC82</accession>
    <name>A0A1E3PC82_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L1c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">RP4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">YL31</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_82909</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042236.1">
        <property type="nucleotide sequence ID" value="XM_019185462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC82"/>
    <dbReference type="STRING" id="683960.A0A1E3PC82"/>
    <dbReference type="GeneID" id="30202708"/>
    <dbReference type="OrthoDB" id="10259820at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.210:FF:000003">
        <property type="entry name" value="60S ribosomal protein L22-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002671">
        <property type="entry name" value="Ribosomal_eL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038526">
        <property type="entry name" value="Ribosomal_eL22_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10064">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10064:SF31">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL22A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01776">
        <property type="entry name" value="Ribosomal_L22e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00077545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00080129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00082610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ63029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="118" mass="13265" checksum="FEF90A3AA4EA47CD" modified="2017-01-18" version="1">MAPITNKKAKAAKKFTVDVSAPTENGVFDPSSYTKYLNDHIKVEGAVGNLGNDITVEENGTTITVVSTTKFSGKYLKYLTKRYLKKNQIRDWIRFVSVKQGVYKLTFYSVAADEEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC84</accession>
    <name>A0A1E3PC84_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein BZZ1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_60880</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a role in endocytosis and trafficking to the vacuole. Functions with type I myosins to restore polarity of the actin cytoskeleton after NaCl stress.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the BZZ1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042041.1">
        <property type="nucleotide sequence ID" value="XM_019183073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC84"/>
    <dbReference type="STRING" id="683960.A0A1E3PC84"/>
    <dbReference type="GeneID" id="30200319"/>
    <dbReference type="OrthoDB" id="8783038at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11912">
        <property type="entry name" value="SH3_Bzz1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000060">
        <property type="entry name" value="Actin polymerization protein Bzz1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035459">
        <property type="entry name" value="Bzz1_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF21">
        <property type="entry name" value="PROTEIN NERVOUS WRECK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="11">
        <location>
            <begin position="4"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="477"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="556"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="436"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="247"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="325"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="468"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00054085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="613" mass="69819" checksum="1CD14580C5936B69" modified="2017-01-18" version="1">MAEVSIGNELKDGFKETNNWVSNNISFLTDVEQFYRQRSQLEKQYATDLQKLTAEYLKKKANKTTAISVGDEPKITPGSLESASLVTWTEILSQTELIAKNHHNLSNEFNIKISDQIVALQKHLTMLHERVLNFHNNSLTKKKDEIFEQVNKAKKQYDETCTTMEQTRSKAEKSSNREKHQKKVNEKQLEMNNAKNEYLLKINIANRIKDKFYYQDLPEVLDILQDLNEFKTKQLNKLLNLSNTLGINNNNKDIKNLERNIQIISENKHHLDTQMFIKHNQVNWKDPQDFYYIPSSIWHDDEHFITSDQELTYLKTILLKANQTNSKYDSILETAREKLNELQNQRLQLKSKSDIQEFELKNSMDTLNGYLFTLSNFIIDENVKVSAQVEIETIENNANDKDLSLDGLRIEKKKTNIFGKLRGSKPKERIVDTHAAPTSNTFDDSHSIRSAKSQHTGFHLPSLRRNRQQSSASSTNNSVSMAMALFPYQADGDDEISIDANEQLVVVSPDDNGWTLVSRPNTGEQGLVPSSYIQVDQSSGGAKKKGPEVTPRKGAKKITYVVALYDYVADGDDELSITAGQKIEVLKGDDGGWTLGEVDGRSGLFPSSYVQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC85</accession>
    <name>A0A1E3PC85_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_88231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041789.1">
        <property type="nucleotide sequence ID" value="XM_019186142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC85"/>
    <dbReference type="GeneID" id="30203388"/>
    <dbReference type="OrthoDB" id="3981132at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="240"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="53564" checksum="0D34845B61EF692E" modified="2017-01-18" version="1" fragment="single">MSDHKNSKSNYDRWKPGTKLACMMSLKQVCQDSRYYDPSNNRFNSLTWHTVAENLNKEYPDYAKRFTNINVKDRYDRVLKHHKVFKKMADKANGFVWNPKNFCFDLNEELIKQYEEGYIKDDVISNELNVVKFLSKPGHIDLEYYHRFVYQFANQEELENDAIIQSPSFIDDAIAKGILNLVTKSNFVSKNDHQNSNEFQSSPVMLQQQPVQHQSSSSSNTTNSSTSIPMAPNSANSQTQIPNSSMPSQSSSSMIPIQQPHLQHHLIPNPQPPHPSIQPQHSSDLDFSGLINPDQSVPQQPPHTHPHPTPMMNPGMTIPFQMGPGGEMIPGPNTQKNSLPLAGQPPFKKPRTASIPGGSSSVVPQSIIGTAMMVDNYESMLAAQQGPAGGQPAGRFGYSSWEAIMQLNKEFDMMRNLVNEQILNLRLQKLISYQDFNQIQTIQNSNDQFVRSAYKRIGDDPKLIDLINIYLNNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC94</accession>
    <name>A0A1E3PC94_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_77006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042051.1">
        <property type="nucleotide sequence ID" value="XM_019184486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC94"/>
    <dbReference type="GeneID" id="30201732"/>
    <dbReference type="OrthoDB" id="3980667at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="403" mass="46420" checksum="87396024C31BA36D" modified="2017-01-18" version="1">MVVVDDAVYPSSSHYHHTTPHHRRFPSIPTIKEVPNRGAFNPTYHKHNAFESSTKQYQHEYKHLNDEGDEIISPEGKAPTIVSTIISPTVTRQHSFKKKKRYMLSSREVKNHFFYPVKAPRRKRSRRGKFRNSVELDASMNYVNIDSSMSKDLCLNQRVKAYEISLPELIKIEPALKETTKAITIDSNNNYIIYTPDPTIGKNSVISDAEQLLDQELLKLGTPITFNKATTPVLPQYKFNRPFFNRYDTYPIAGSHQLLRTRSLPSKWRKEGLQDLWNLYLRRVVAARIMWRLKHISSEPNLKRARPSFSSSVDTGFFINSLLKNNEMLPAHNSKSSSGTMSDDEESDSDVEEPITDDDHESRIENDDRTSHTSRGSHRSRESTQEVLSSLESLLDDMRSIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCS9</accession>
    <name>A0A1E3PCS9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_60744</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041894.1">
        <property type="nucleotide sequence ID" value="XM_019183051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCS9"/>
    <dbReference type="GeneID" id="30200297"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="669" mass="76846" checksum="922C1379EFE1CB1C" modified="2017-01-18" version="1">MFTKISGRKKYIWSASLLPSTDFNQRWSSSGSKALKTLSSNKLVSSFSSKTNTIHSKYLFTKSEKGYISQSDKYSTINSQTSELPPKLNYDDYLHSDEKPTFEGLAEFINKYIISRIIKDESFSEAATSIAARIFMSSPSPTRSGWKRFPYHNQNQKLLSIEEGTKFRKISEVEILNQFADKSNIKDGIAKVFNDLQQYYFKKAQDDSNSHEKRIINIFDMVDSMRKLPTALREPANKQEIQSLSLSYVIHPLVQVINDVYDSKLVVLQQRYVSGRKSPDVIIQDEDSKAFSVLEFENNLPKAENIKSMKSAELLDLQGFKQTLYSIAMSNTNMGVLSSFGTTLIIEVDWKKSCFDGTDTCKLALELFIIDHNEPLTTLKSTIMHFLTKLLVLSTSEEKDKIMKTSLESRTKMIINCEETQQKHSQQLRKSFLRQYQNDSRYLNKEVIDVRLDLQDDKSSTDLKNVFRDMTEKAKYIILKKKHFLANNIVDAVDDDEEVVVKWYEPITHELTDNPLLNQELLEQDYLDEMYELKKLERIENSKNLELSKLRLFKNGFMIIKDQYSNIVAIGNYLAMANKNPKTNKNEITKRLPILTKSDILSEAMNRLECLHSNGFALNQNVDQRVVVNNGDVSILATTKYGDEVPDRIKDLDTKTLGQIVASGRIIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCT8</accession>
    <name>A0A1E3PCT8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_82904</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042231.1">
        <property type="nucleotide sequence ID" value="XM_019185460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCT8"/>
    <dbReference type="GeneID" id="30202706"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ63024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="113" mass="9855" checksum="074C8DABF40FDF46" modified="2017-01-18" version="1">MASSLASAGTAAVGSAGSSAILGGGSSSGGGSGSSASQSSGGGSSASKSSGGGGSASGGSSASKSSGGSSSGSSSSGSSPAQGVYVQNPISWKFGYLYGGILAAGFVLTAGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU29</accession>
    <name>A0A1E3NU29_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongin-A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_48356</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454216">
        <property type="protein sequence ID" value="ODQ56691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035898.1">
        <property type="nucleotide sequence ID" value="XM_019182609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU29"/>
    <dbReference type="STRING" id="683960.A0A1E3NU29"/>
    <dbReference type="GeneID" id="30199855"/>
    <dbReference type="OrthoDB" id="21513at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070449">
        <property type="term" value="C:elongin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051870">
        <property type="entry name" value="Elongin-A_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010684">
        <property type="entry name" value="RNA_pol_II_trans_fac_SIII_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15141">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15141:SF76">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06881">
        <property type="entry name" value="Elongin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="211"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ56691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="36778" checksum="A0829BA4E07BC65E" modified="2017-01-18" version="1">MPVFPLSVLCENILLQNYLALKDLGDTRFQLIQRVLLRFNPQQLIELETSNPRLMLDDESVWLSLVKKEFPQDVHERYTTNSSKIKDFFKQQLDEVGCDYSNINLDNYISYQQLGNTKKYKLPSKLLYLKYREDYLKKEELAIENLRQRMKAIQKDKEENRVVHIDDVIPEPNARVKNIAKPQRSKIFMKSKIEAKQRQTLFKNPLRMSIEGKTPVIRPPRKVPTPFITKPSSPRPKPILTSPKRSKPASPIRPKPQSSSVFHNKRAPRLKPAQPTSPIQSTTVSHAENTSQLTENIPNEPPRKRINIGEYKARKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUM7</accession>
    <name>A0A1E3NUM7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_14307</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036096.1">
        <property type="nucleotide sequence ID" value="XM_019180603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUM7"/>
    <dbReference type="STRING" id="683960.A0A1E3NUM7"/>
    <dbReference type="GeneID" id="30197849"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990317">
        <property type="term" value="C:Gin4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032186">
        <property type="term" value="P:cellular bud neck septin ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061163">
        <property type="term" value="P:endoplasmic reticulum polarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010458">
        <property type="term" value="P:exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097271">
        <property type="term" value="P:protein localization to bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="5">
        <location>
            <begin position="14"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ56889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="313" mass="36055" checksum="C3B7AAA61A011692" modified="2017-01-18" version="1" fragment="single">MASTALRYKNDAKRGIKYTIMITGASGTGKTTFINSLLDTKLLPHRYQPIGSSIEEPTLVSYIGDSDCNYDSREFNPLRQYTESTMSITSSKIEIADDDGSKVMLTVIDTPGFGDNIDNELCFAEIVGFLEQQFDHVLAEETRIRRNPKFEDTRVHICLYFLEPTGHGLRELDVECIKKMSKFVNVLPVISKADSFTQSELHAFKRNVLYDIKKYNVPIFEFNYDEDEDDIETIEENKVLRNLQPFAVMGSKEKFDVNGELKYARKYPWGLIDIEQNSDFLKLKNVLFGSHLQDFKDLTTNFLYENYRSEKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV09</accession>
    <name>A0A1E3NV09_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_58505</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036173.1">
        <property type="nucleotide sequence ID" value="XM_019182824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV09"/>
    <dbReference type="STRING" id="683960.A0A1E3NV09"/>
    <dbReference type="GeneID" id="30200070"/>
    <dbReference type="OrthoDB" id="10257314at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000008">
        <property type="entry name" value="Alpha-ketoglutarate-dependent taurine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051323">
        <property type="entry name" value="AtsK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468:SF9">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT TAURINE DIOXYGENASE (AFU_ORTHOLOGUE AFUA_3G01010)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="8">
        <location>
            <begin position="117"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="376"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ56966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="413" mass="46792" checksum="335A0A923882EA07" modified="2017-01-18" version="1">MTTSTTESQVNDIVTKYNKLDLTKSTQNATIQVELKNANGFQSGFGDEYSSELPESTKKRFAKYGIDISKGYPERPENIITFLEDAYKIRDSANPDYIERGVNADLEKKALFAKAKEVRNLTKYVGTELVGVQLSDLNEQELDELALLVSERIVVFFRNQDLSPQNQLRIGEFFGTIERHPLAAQVPGHPGITTIWGKYNRKNADKSFKKKGGRAWHTDLDHEFQGPSLTHLHLDAIPESSGDTAWTSGYGAFDKLSPEFQKFLEGKVAIHRSAHKYYERDNILGGSKHIEREHPLVVTHPVTGWKALFVNRSHTVRIKDLEPEESQVVLNYLYDVLEKNLDIQARFTWAVPDQDRSLGASAIWDNRVSNHYAIDDYDDKEDPRHGTRVTSLGAPPVFKENSKSQREALGLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV86</accession>
    <name>A0A1E3NV86_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_81247</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036222.1">
        <property type="nucleotide sequence ID" value="XM_019185202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV86"/>
    <dbReference type="STRING" id="683960.A0A1E3NV86"/>
    <dbReference type="GeneID" id="30202448"/>
    <dbReference type="OrthoDB" id="62798at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF275">
        <property type="entry name" value="LD06095P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="329"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="347" mass="39451" checksum="533B4BECE15C3548" modified="2017-01-18" version="1">MSIPLFLFTFNCAKLPQTDFSKLQDALPSDHIPELFVFGFQELVPILDSTIPKEVNQRLFEINDSIHDVLSSKYPDSSSIHTINIHHLGAIGLIIMTPFQSSIKKIDNTVASCGLFYSSLKGAVATRLTLVKDSQETEFTFVVAHLSANEGYVERRNRDSWRLLRSLSFNDGWSVLKPKNHCFFMGDLNYRVALYGDELKRQIDESKVFIGFNEQDIHFDPTYKFNVGTSSYNSKRIASWCDRILYQKYNGNEKLIRYDKLPFLSSDHQPVFLSILVPFQAPPNAVNSLGYLIEGGEFMKSTYASKFGNYALIVSDSIIGLSLKSITTTSGRIVLIVMFLFILWLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVE9</accession>
    <name>A0A1E3NVE9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GSKIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_65339</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036282.1">
        <property type="nucleotide sequence ID" value="XM_019183892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVE9"/>
    <dbReference type="GeneID" id="30201138"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2280.10">
        <property type="entry name" value="Hypothetical protein (hspc210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023231">
        <property type="entry name" value="GSKIP_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103107">
        <property type="entry name" value="Hypothetical protein c14orf129, hspc210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="13342" checksum="F590FFF94AEEE4FB" modified="2017-01-18" version="1">MSSEPQQIIKEYQSFFHSIQQSPNDPQVLKIITLENIEMDVEFSNNGWIFNNFEIFEIFENGMMLKSEGFKRKFHDVLYEKLILEVISIEFVKGLFMTSIKSNKPSSIKDLNGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVF2</accession>
    <name>A0A1E3NVF2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sister chromatid cohesion protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_71124</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the SCC2/Nipped-B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036386.1">
        <property type="nucleotide sequence ID" value="XM_019184232.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3NVF2"/>
    <dbReference type="GeneID" id="30201478"/>
    <dbReference type="OrthoDB" id="418242at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090694">
        <property type="term" value="C:Scc2-Scc4 cohesin loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061775">
        <property type="term" value="F:cohesin loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140588">
        <property type="term" value="P:chromatin looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034087">
        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071169">
        <property type="term" value="P:establishment of protein localization to chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23958">
        <property type="entry name" value="SCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026003">
        <property type="entry name" value="Cohesin_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024986">
        <property type="entry name" value="Nipped-B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033031">
        <property type="entry name" value="Scc2/Nipped-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704:SF18">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN DELANGIN SCC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12765">
        <property type="entry name" value="Cohesin_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12830">
        <property type="entry name" value="Nipped-B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Sister chromatid cohesion C-terminal" evidence="7">
        <location>
            <begin position="1236"/>
            <end position="1416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1512" mass="172665" checksum="2C8EDE6AF4C8DD40" modified="2017-01-18" version="1">MNGESKGVKSDQFPVNLQEGLGVTPLTYLIPTQDLAALILPTSLTPISPEINEEEGCELYQENVKAVFESNSPSYDSVKQNVLETYSFSQCLENSTLSFRGTSSTIEDETTHETKSLFDGLHGLAKNVIDSVPLVSHKNIVSSNKDAPQTVTTSRPNPKRTASTLIQVQNEVEIKKRKSDIGVQHLQLESISNQKLTDLVVNKIGLADEQCEYESSDLWIELPSSEHVLSDDVFVEVEQYLLKVKGTRLVQEFSFEVLDRLKKLSVRSIKCSENFDWADVDLQSDFWPLKIALNSLKAVTTILIIYNCQRKEKKLYLDEDLVLALDFIYKLTEDLILKLTVQSDEWDNENVQLIIGVMNLLTKNLELFGDYIASNQANDTVLTKLEYLSLMIFFHAPEKNKFTTSMQSLKTSSMNLLIDLFRSKKDQRSFIVNEVLSNFDRLPTHKTQSRQLKLFRGLNIQFFTASILNFIQSLNIRDIELKETIKEKQQHNRDAGLADFIEENNEMKRITEELQTEKNSILSLISSTLVNRIITQLNANTKQLFELFIQDLLNVVQFPEWSASEQLLSSLTNTLVSNSENQPSIVETYLLEIIGLIGSTMLELKGPNDLPDLSVSDLTDEYSIVSSYLLTLRDAFPVKFSGDSFVYDWIYKLSSNISSSTEEDYAKAKERETYLKLLSSYYKEQSNGLIKKNDGSATEENATLCYKKILLSGTFMKLYHNFLTHLLRSIDHPKIKSRTRAMKNLAQLIDKDSRLLNLPMVKQSLSNRIADPSPLVRLAVLEIFDQYILKKPELIGEFYQTLILTNDKSVSVRTKSLKIAKRIFLEASNKDIKVFALEKILRRLEDDEENVLDLTKQLLMETVFLGLYDQIGAYSKDQIKQRDAVSFMIDILLDLINKSEKTWELFEKYLNEDIMRKTDENKKFHNNLLSISNTIVDVLLTFVVDYVDTEKQDSVEKSLGLLSIFSKCEHPLINQEQLMSLQPYLTSESTVSSSTSYYTLIIFRHTLGRTNSYRDQFLTDSQTSLLRRLTKFNTRELDEAMPCIWMISTMKKDTTKISNAAASCLTLIKPNVDKALKDELDKSDPKVLRLIYLIGSFGKYCDLEANRQVFKNAKKLALKDKESVLSLITKYLLIFTRDSVNRQIRRVAVRNIINVCSSHPKLFMSSPVLNVLDKEFTGSSIDFKEVMIQGIFEFLEREEKKSSKRSGHEGKISSEVSLDVDVFHGNSKMFENDGICASLVQRYMDPILEMCTYDDGEHSYLAVKYLKFIVKLGFANPRICIPTVVALEASKVPYVRSMALEIHRELHSKHESLIDSSYIHGFKLAVKYRAKLSSGLLTENQQYFQKFYKVIEESKATKKKIFNQLVKSFTFSIDGCTEEQALFMKNYVLFMANTIPCCELSTLEEVFILVDGIDAVLANQGVTLSSNVSVKLNQTDPNFDWKIYGYLSLAILSLYELKNCLIQAYKIPEDGQRNELKSQKTKFTEVPIKLFDISPEAGSFREICEKLATILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVG3</accession>
    <name>A0A1E3NVG3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Inner kinetochore subunit AME1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_65285</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036227.1">
        <property type="nucleotide sequence ID" value="XM_019183886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVG3"/>
    <dbReference type="STRING" id="683960.A0A1E3NVG3"/>
    <dbReference type="GeneID" id="30201132"/>
    <dbReference type="OrthoDB" id="3995136at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048743">
        <property type="entry name" value="AME1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20994">
        <property type="entry name" value="CENPU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inner kinetochore subunit AME1" evidence="3">
        <location>
            <begin position="205"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="224"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="73"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="106"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="124"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="322" mass="37422" checksum="16AF16CB05ADA65B" modified="2017-01-18" version="1">MERIEKREARKRGAASRQLATNDIVIKTPKRSRKSRPNTPKWIPVSQDSGALITDELSQLHSPISRNTRPPRTPKDKINEEYEPRVKKPKAKPKKVKPRKVEVKPKTKTKTKPKSKRKKVEPQPDNDLDNDLDDDQDQDFDNAQEESDIELDDLYVNGTMEVKISRLPTSSIRLTSIDIILQSLNDALTHEDGKHSQIFQKLNKLHLNKILDLNLTNNHYLYKLKDLKSTKLDLRNELFDKRQEINENLVKLENLRNQYNKVHQLIKTKKQIIGKLNNLSKSSSDNNDSILMKLNKLNNIVDPNWGILDKLKEINSKFHELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVI4</accession>
    <name>A0A1E3NVI4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="17">ATP-PFK</shortName>
            <shortName evidence="17">Phosphofructokinase</shortName>
            <ecNumber evidence="17">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">WICANDRAFT_107462</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 17">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 18">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="17">Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 17 18">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterooctamer of 4 alpha and 4 beta chains.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 17">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Mitochondrion outer membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="17"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036330.1">
        <property type="nucleotide sequence ID" value="XM_019180505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVI4"/>
    <dbReference type="STRING" id="683960.A0A1E3NVI4"/>
    <dbReference type="GeneID" id="30197751"/>
    <dbReference type="OrthoDB" id="537915at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000010">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000007">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000008">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03184">
        <property type="entry name" value="Phosphofructokinase_I_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009161">
        <property type="entry name" value="6-Pfructokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02478">
        <property type="entry name" value="6PF1K_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF4">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000533">
        <property type="entry name" value="ATP_PFK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="13 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 17" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15 17 18" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="12 17 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="14 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="19">
        <location>
            <begin position="182"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="19">
        <location>
            <begin position="572"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal catalytic PFK domain 1" evidence="17">
        <location>
            <begin position="1"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interdomain linker" evidence="17">
        <location>
            <begin position="558"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal regulatory PFK domain 2" evidence="17">
        <location>
            <begin position="572"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="254"/>
            <end position="255"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="284"/>
            <end position="287"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="330"/>
            <end position="332"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="374"/>
            <end position="376"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="459"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="465"/>
            <end position="468"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <position position="642"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="700"/>
            <end position="704"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="738"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="745"/>
            <end position="747"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="831"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="837"/>
            <end position="840"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <position position="918"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR000533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ57123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="947" mass="103879" checksum="1B51C30C6C755DCD" modified="2017-01-18" version="1">MTVSTPIVNGTSYYTLKANTKESFDEAVQFYENYIGLIPKNKTSKSIFLHSGQEFPHHSATIELVLDESFKQSPNFKELQQERLTQDWRSLRSNVVFRTDSIESLYELFKNHPNVQVQAYPSDLAPTEIYVLDPLGNLIGFTSLRHPLSTVPPAYKKIDQDILTTGPTTAYVESATSEQRRNIAVMTSGGDAPGMNACVRAVVRAAIYRGCKAFAVYEGYEGLVKGGPNYIREMDWQSVRGWLSEGGTNIGTARCMEFKQREGRLLGAKHLIEAGIDALIVCGGDGSLTGADLFRAEWPSLIKELFETGQITKEKFDKHKHLNICGTVGSIDNDMSSTDSTIGAYSSLARICQAIDYIDATANSHSRAFVVEVMGRHCGWLALMAGIATSADYILIPEKPASSREWEDQMCDIVSKHRSRGKRKTIVIVAEGAIAADLTPITSKDVLKVLVDRLGLDTRVTTLGHVQRGGTAVAHDRMLATLQGVEAVKAVLESTPDTPSPMIGIIENKICRRPLIEAVKLTKQVAEAIEAKDFKKAIGLRDSEFVEHLNNFIAMNSADHNEPDLPEDKRLKIGIINVGAPAGGMNSAVYAMATYCMSRGHTPYAIHNGFTGLARHESVRSINWLDIEGWASRGGSDIGTNRTTPEEADIGLVAYYLEKYQFDGLVIVGGFEAFVSLHQLERARNDYPAFRIPLVLIPATISNNVPGTEYSLGSDTCLNSLMEYCDIVKQSASSTRGRTFVVEVQGGNSGYIATFAQLICGAQASYVPEEGIPLEQLDLDIQALKQSFQVAEGKAKSGKLILKSANASKVLSTDVIASIITDEAQGRFDAKTAIPGHVQQGSLPSPMDRTRAARLAVKAVQFIEEKQEFTQPVHYAEEFDPSDKKVGDTAAILGVKRSHLRFSPIRQIYDFETALNKRMPKVVHWEPIREVADALVGRTKVPTTSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW73</accession>
    <name>A0A1E3NW73_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">nitric oxide dioxygenase</fullName>
            <ecNumber evidence="4">1.14.12.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_86265</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 nitric oxide + NADH + 2 O2 = 2 nitrate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19469"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.14.12.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2 nitric oxide + NADPH + 2 O2 = 2 nitrate + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19465"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.12.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.12.17" evidence="4"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036034.1">
        <property type="nucleotide sequence ID" value="XM_019186027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW73"/>
    <dbReference type="STRING" id="683960.A0A1E3NW73"/>
    <dbReference type="GeneID" id="30203273"/>
    <dbReference type="OrthoDB" id="436496at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008941">
        <property type="term" value="F:nitric oxide dioxygenase NAD(P)H activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070458">
        <property type="term" value="P:cellular detoxification of nitrogen compound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071500">
        <property type="term" value="P:cellular response to nitrosative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046210">
        <property type="term" value="P:nitric oxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19754">
        <property type="entry name" value="FHb_fungal-globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.490.10:FF:000003">
        <property type="entry name" value="Flavohemoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396">
        <property type="entry name" value="FLAVOHEMOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396:SF3">
        <property type="entry name" value="FLAVOHEMOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0216">Detoxification</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Globin" evidence="15">
        <location>
            <begin position="3"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="16">
        <location>
            <begin position="154"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ56827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="395" mass="44419" checksum="2E0CFD89676122B5" modified="2017-01-18" version="1">MSAITTSQKKIITASIPFLKEHGVDLTANFYKYMLETHPEVRPFFNKSHQITKKQPKILAFSLVKYAENINDLTPLLGFVRQIVEKHVGLQVTPEQYDIVGSCLLKTLKEMVGEGATDEFMEAWKVAYYDLANILIDLEKKRYATELTTKDAWLGFKDATVSKIVQESDSVKSVYIKPNDEKLANFYPGQYTTIRISTDSGATVQSREYSLSNEYPELQNGLQYYRISVRRIPGGVVSNYIHDQLKVGDAIKISSPYGKLLEPHFNAVKESKDDSKPVVFFIGGIGITPSISSIEYFLKQGKKVQLYLSNSDVNSRPFGNWLERLIQEYPTLLSVDEFISTTADLAGAFTNENVQNNDYYLIGPLGYMGLVTEFLQSKGLDVATINSEQYGPVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWA8</accession>
    <name>A0A1E3NWA8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuolar segregation protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_80747</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036612.1">
        <property type="nucleotide sequence ID" value="XM_019185117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWA8"/>
    <dbReference type="STRING" id="683960.A0A1E3NWA8"/>
    <dbReference type="GeneID" id="30202363"/>
    <dbReference type="OrthoDB" id="1204at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010513">
        <property type="term" value="P:positive regulation of phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903778">
        <property type="term" value="P:protein localization to vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024260">
        <property type="entry name" value="Vac7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28258">
        <property type="entry name" value="VACUOLAR SEGREGATION PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28258:SF1">
        <property type="entry name" value="VACUOLAR SEGREGATION PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12751">
        <property type="entry name" value="Vac7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="567"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="527"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="696"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="539"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="706"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="774" mass="85970" checksum="206EF73EBFD069FC" modified="2017-01-18" version="1">MSDNESVKHSTVNEKDEASVTKSNLNASTIASQEGSINNNEPTKPVNNDEIIPNIPKNLPQIPPSKKKELSRPLFSRSSTNEKVSKNPNTTKNMIVETETVDASKVPSVTLPIASSSNVISNELMSNNSSIANNLSSLRSKKSTLFVDQNNQNNQIANNNVQALSTDDNDTISINGSIRNKRPNATESHLNLSKPGATKADLFAARIANAVGDASDSDSEETFVYESAPDALNTANTLPLNKNSSISAVNGSGNPMALRKLSQRNISMPVTSSSSQPYSPTTASHIVLTNDESKDQNNGQNNGRTFETEEESDLNNSNNTTITNIQTLQKDQYQHNSSDNVSDLQGDKSSAIEGSLKADTNTQSSFTESGNLINNNNNNKTKIFTKYNGNNSRKPSNTSLLRAGNGDRQNQLRTTTSKLFEVKGSSLRRYSGVPDDVNIEDYIDQYDEEQEIDGGDYADTYEYDDYEDEDELTPLNVNAANGNLRLNNSRNYHTKKRFPNFRSTDSGDYTQNFGDLKDKNSLRHSNSRILKNNKKNKNRSQYNQRQYSPHNFYNSKKNSKLQLFKNFLYFFVLVLLLLVFGFIAGFLLATTKDLQDVNILSIDDILVSYDELVFDLSVRAFNPGFVTVEVSDVELDIFAKSSHLGNEYFAPDYLDDSKTLQTILLGSVTKFETPLEFSGGFFNRNHSLSKGEIKLLQPGKNSTDNGDEHSAPNHKTENDSDKWKTIINYPFDLIVRGSFKYSLPFFHTDRSVAVTKTVSIDPAEDKKYKTGFGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWK0</accession>
    <name>A0A1E3NWK0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_107411</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036269.1">
        <property type="nucleotide sequence ID" value="XM_019180500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWK0"/>
    <dbReference type="STRING" id="683960.A0A1E3NWK0"/>
    <dbReference type="GeneID" id="30197746"/>
    <dbReference type="OrthoDB" id="125856at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009976">
        <property type="entry name" value="Sec10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048627">
        <property type="entry name" value="Sec10_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048625">
        <property type="entry name" value="Sec10_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100">
        <property type="entry name" value="SEC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07393">
        <property type="entry name" value="Sec10_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20667">
        <property type="entry name" value="Sec10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec10 N-terminal" evidence="7">
        <location>
            <begin position="52"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec10-like alpha-helical bundle" evidence="6">
        <location>
            <begin position="175"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="483"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="813" mass="93918" checksum="D8DA7ABD6A84A18C" modified="2017-01-18" version="1">MNSIYDLDPKIRKLLSLDNFLDNLTVYEFIEELSKDHSLKSTEVNSLKYLDPKPFIRTFESTLRELNQLSTEAASRQSQLEKEVSKYELDHSRNVLSLTSRAESVNSQFKHLDDQISKVNSTITPLSQSLTKTINAKERSIATISLVRIYNDFYQTGYSKELDSLMNGSIKDKRKCSATVAQLLSLSQKLISDDLERSAKCHELIQKYSETMENSLLEEFNIEYKANNLTKMKDIADILTDFNGGVAVIKNFVNQHDFFLNANSTEEDDGLTERFEKDGIWEKLSDPLNHHVLTDLFTLEIFKETENVIRNETGVIVDVFREPVAVLQVFVQRIFAQQIQFKIEQLLKNSYATSTLAYLRTLNALYITTGNFTKNLKNFFQDSLQRDVRLDELNIILDQSFSDLFTSHLIDSKYFDLEKKTLESIFYTITSKYEQLNESKIQNKLSSRLHNNKSIEPNNEYENLNRRSRVGQFKSFMRSHLERSQSFKDKRASDPSLANDHNHQNEIDQYSKLNLEHTDNLLKSTVESLSRLIELTPTKMAEHGLEILEILLIGIGKSYVDLALEVSFNELHHQDFKYTDHYLEFDYLQNVQISSEILYLVSTCIRTILLPLANNSPQVKHRMINLTNGYISRVELAINVIMRDTINLGCDKVRNALSKQKKKDFLPKVGELIDTDTVACEQLSTFLSEFNSQLTHYLNGGNLQKILLELGVFTFNQLFEHFKMFQINSTGGIVVTKDIISYQTSIEEWKIPELTTKFQLLREIANLFTVQPELLNSLTKEGQLATVKPYVIRQFIMKRSDYSSNSYVDRLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWK9</accession>
    <name>A0A1E3NWK9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_80860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036733.1">
        <property type="nucleotide sequence ID" value="XM_019185134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWK9"/>
    <dbReference type="STRING" id="683960.A0A1E3NWK9"/>
    <dbReference type="GeneID" id="30202380"/>
    <dbReference type="OrthoDB" id="4035999at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="518"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="551"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="570"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="853" mass="96377" checksum="FB984B4CAC2D1B59" modified="2017-01-18" version="1">MSLKSPTTELLFYESPISTLLYEIRTSEQHYRDQLSKLLGCCSPLKRQAANGFTNSVRKLIAYHTDLVRSLDHVDEVQSIYNFIQWTQNSIPVYQNYVANYQLDANSLQIEIQLKKNPLIRINQLNKYFNKIIEINENHTGEIISFDSKISQNLMKLMQMAKDKNEMENLRIDLNTLLFNKVHSLDLQFSNTVGFFQLDQSIKRDYFKFDLLHPKSTLVDTLVEVFQLSNNTLAICSVESIGRSLMFPPLKNGDFEIISNDLNLIHLQNDDDVELKFTCPDKSQLSYWNGIIKNLFNNNGDDVGSNFQKSHYKLQDLKNDKMGLGIKLDDNQSIGSLLRRSSPILSKHTSLASDYSNQILSEISPNLSNIDQFFAHDADLNKLNSYTSDQDAKVARNNSITPRLNSVPRSTTQDSFSSGEEITSKNVESIGTPSPTLPLNIKKSIKTTNLNLQDLSNSSTVSLPNFQSSQPNLLNLNTGSSIDLSSKFDKPKLKKRRSIFNLFTKKLNNEPEFEIVTKESTKKPELETSSAINPQPSQSMASLQKNVKNLTISTSSTSSTTESPLPSPFARSNTKQHSLTPFEETTLASSTITRVLKNNTIVSKWSQSHWESIGEEYGHVVKFLSSSQGRLFVLYQGDGSIIPELMIPTEGACVNKSSALDLQIKCYQSVESKKMMMLNVRVKDVYTLRLIMNEFENKETLSASNSDVFSAPSNSTSMSSVNVDEIQELGKPYKNGHVGSGSSTSLHTKTLLLKSDVKIKLHKLDQDEEWRPISIGKFNISSNLGNPEYSKFEINCNDKTRIETIVKNSNCSRIGRTGLTFNFVDDELEIDQVNYLIEFRNSQECVEIYEMLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWS0</accession>
    <name>A0A1E3NWS0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_80952</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036828.1">
        <property type="nucleotide sequence ID" value="XM_019185154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWS0"/>
    <dbReference type="STRING" id="683960.A0A1E3NWS0"/>
    <dbReference type="GeneID" id="30202400"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047134">
        <property type="entry name" value="RNF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23041:SF78">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23041">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="148"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="214" mass="24403" checksum="2C0AF451674F8CFD" modified="2017-01-18" version="1">MVQETHGNKRSRSPDEGRTQNDQQVKNKRTKPSNQVNEEANVIIPDEDEEDIPYTHNNSRSTTPLSDLSSENEDEIQIMREATVENNNTPAQELDIVDLDDDDLKEYFVISEDEDNKEQEIENSQRTVQEQLKDTSRQNTSNLKNAHCAVCFDSPEHTYILPCGHIYCGDCVFKALSSTKQSNRTGGPCSLCRTFTSYRKVTVGIFKKKRKVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX98</accession>
    <name>A0A1E3NX98_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_36424</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036414.1">
        <property type="nucleotide sequence ID" value="XM_019182434.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3NX98"/>
    <dbReference type="GeneID" id="30199680"/>
    <dbReference type="OrthoDB" id="289913at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059070">
        <property type="entry name" value="TPR_VPS8_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025941">
        <property type="entry name" value="Vps8_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 8 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23410">
        <property type="entry name" value="Beta-prop_VPS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12816">
        <property type="entry name" value="TPR_Vps8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25066">
        <property type="entry name" value="TPR_VPS8_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23413">
        <property type="entry name" value="zf_RING_Vps8_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 8 central" evidence="3">
        <location>
            <begin position="592"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="VPS8-like TPR-like repeats" evidence="4">
        <location>
            <begin position="1109"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1336" mass="152054" checksum="0E06373026203F9B" modified="2017-01-18" version="1">MSASVSNNSTPRKNIEEVSFIPDTASFQSGSTFRATPTKSIKRHTFGSPRFPSNSSLNSSISRSRRFDERLTSRFSDKARETHTLDLHQDDEDNHEEANITSIKWVNLQKLNGCLHDGKAKTLYGDPTCIVPGDVVAIGTSKGYILIFDYHQNLLHSLGKNTKAMTCGSITSIAMSLDSTHVACGFESGDVFLWNIRTSSKPLCHIQPLNDTNMHSDGHVAGSIVNNLTFVGKRHTVLISSDVNGLVFYHQCNRGVLGVTVGTRKILGRYEDIQLRRPTTVFGCTALPLGPSVELLDAQGLVAILSSSALVIISTSNNIKTQFKVGKPKVIDQSVGKTGCLSWFPAFLHKNGVKSPAKLAYCWSNVLTVLEVTNYGEHLQFDSKKRWLCDEAILGVQWVSHSVIGLITTTQQLILITERTMKVSASIDLLSRHIYKNDIYSRMPTHSTLTNNFNSSYRIYKGKMFLVGKYEIFIGSTPNWMDVLYEKVNRGQYIEAIEKARKFYAGVGDLDVVGLPREFNDRQKIVLRYLVDIVKASATHILTPERLNNEEDTVVKDFLYTSIKALISIHADSVVYEDFYETFKVNNHVDYFFESLEPFILGGLITSLSPIVLKDMVAYYSSAGKTEVLEQLICLLDIKSLDIDFTISLLQKNNLRDSFIFIWNNLLNDYITPFVDFVKDIQEGKEDSYKVFSYLAYTLTGRQYPTENLIEDSRAYEAKLSLYYVLFNGMAITWPKQGGSLIEVKSENNEKFPYLLLLLRFDSSSFLSALNECFEDSLLNDDEIIHEDFKLKVNRQFIIEILIEIYNQNEFSFEDQLSLSIFIARNYPKFQQFIRLSESVLESSIQKLCSYEGDDLKEDCELSLQSLLSVYQPSNRDELLSTFQNAGFTDVLMNLYLNDQKYADVLSLWLKSNKSNSIETTDDILERCFRSTENKPKERANVEKVVNDNFADLIEANTHKIVKILNVYSPKSHKLVLKLDDLHLKHLYLSSLSNLEENGQFKMSKEERDEYIGTLAKFDKKELESYVKSLSLSDFDFKKCVDSLKENEAVDTLVYLYKQQDRYEDALSEVLDYMQKLVNNLSTLQADYEKGKFQSVESKLWKYLLLGINISNHEGSTTQTRVSSELSVNEKMWLSLINFTVNLFKKFDKDGTSSKALDIIKRLVQDLFTSLINTRAQISSFKVNESGVNEHQSSFLKIFAKFLESSSVHVTLLGDVRSVTNEIYLAFSYEKNILTITSKLINEGIYQNMQKLTDKNLAGWSVLNFECDACGKTIWGRGIEAEAYCKWEDRQRGKTIEDGDVYDLVSFSCRHCYHKRCLENLGVKENLTCVICDNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXD7</accession>
    <name>A0A1E3NXD7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sfi1 spindle body domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_81545</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036539.1">
        <property type="nucleotide sequence ID" value="XM_019185252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXD7"/>
    <dbReference type="STRING" id="683960.A0A1E3NXD7"/>
    <dbReference type="GeneID" id="30202498"/>
    <dbReference type="OrthoDB" id="4070448at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013665">
        <property type="entry name" value="Sfi1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018907">
        <property type="entry name" value="Spindle_body_associated_C_dom"/>
    </dbReference>
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        <property type="entry name" value="Sfi1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Sfi1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sfi1 spindle body" evidence="2">
        <location>
            <begin position="320"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Sfi1 spindle body" evidence="2">
        <location>
            <begin position="487"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="Spindle body associated protein C-terminal" evidence="3">
        <location>
            <begin position="890"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1019" mass="119749" checksum="59A98679441EA615" modified="2017-01-18" version="1">MSSNESKGTGRPEAKSPSDTLSTDALLNSFNRLNLDHRVGVGGGVDDDEANPFLDTTTSYIDANLDNLRGFRRDLKNIATSTHNNISESTMEIINNNPITPLKRSVSGSSLLESHSPEFIPSAGAAESHKRSNSVPYEEPVLDLELLFALLNQLPDIERYNNFKGILEYYLQILDEHEMKFGNDRFIITIYNQLQKLGHRSKLEKVIDIFLNEPSNFAMKLSNHLYFKDLAIRQKFLRIWLIKRRINHELNQSWKIWMLYLKKKHFSQWTYKHKVITEDLESNALIFNNVKQMMKVWDVWTANFQEQQAFARVADLQQESKFFSDWKERIEIDNDKADSFYQDNLLQRNFKIWKLSTVASKSNDVAGLKSFYQKWKSKQDRVVELQENGAQLEQIFHAGFFLSKWKARSQKTLSKTRQLEDIFNHSVMSKYLNKWSEAMYFKSIENQVLNKRNSILLGFTLNQWHQRSKHKEMLDSFKYYQGKVHLSKFLSRWKNQLVLKRKSSEVGNYTVLKTHFNIWKLSTNLKIVEGTKNYNSVEKLFNKWQRRTQLQKLSNDYNQSILSKFWDKLVLQKIKLDNSLEAGDEAYESYSKVIYFNHWKGQLDQVHENEVRSDNVVLKKFFQKIVKRNSDGSTLEQKAIEFHSNKVSKTLMSQTFKKWSDKYEDSLESKLEMKLSEYNANIEKLKLSSIFKTWKDQYESYLSQEELFKEEFLGGLYLKPTFEKWINKFNSIREMEDKADDVNNVNLLSTGFTKFQLAFLKIEELENKLIEFEDDANVKLILKYVNTWSLRLLKVKRDNENISSFQRRWNRAHCRAILLLWKGKVLDRQISKFKDVDEVDEEEEDDSPTKFKSTFNSVLPQETPRKSIIHANYSFNSDLGDKSPLAFGTNKKTTTSIPGSERIKRKRIEALKSHYSQIKYAIPSPLKSERKIINNSSQSSKRLDFTQHTPRNQFFQTQFNQRNSNNFQYGAELDDSFSTEINEDQISSPVISHQKINQQFFNRYVSKNRSDSDGGFKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXR1</accession>
    <name>A0A1E3NXR1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_94977</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037130.1">
        <property type="nucleotide sequence ID" value="XM_019186477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXR1"/>
    <dbReference type="GeneID" id="30203723"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="75" mass="8443" checksum="CCC593F27F747C4C" modified="2017-01-18" version="1" fragment="single">MSFNGLLRLASRSFSAIKIQKNLTRKAQIPNAVIQVADFEVTKLNREIDPPALDTFALFCISFHILHTLGHVKVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXS2</accession>
    <name>A0A1E3NXS2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_85163</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037188.1">
        <property type="nucleotide sequence ID" value="XM_019185852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXS2"/>
    <dbReference type="STRING" id="683960.A0A1E3NXS2"/>
    <dbReference type="GeneID" id="30203098"/>
    <dbReference type="OrthoDB" id="413079at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000286">
        <property type="entry name" value="MFS efflux transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051788">
        <property type="entry name" value="MFS_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23514">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23514:SF6">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="367"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="402"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="467" mass="51592" checksum="0562FBBF42D70473" modified="2017-01-18" version="1">MTEQGKPIQLDTMNHSTLADYTTSNFQTLSQVNSERNSTSNDASQRIDNTKPIMTLNNPPRNLYRVISACSWGLGTGMTAGVIGSLLPFIEAYYKVNYAIVSLLWLGNALGYILIGLIGHHIDIKIGKWKSLMVGAICHIAMSSIMITGTRFPAIIIAMFLGGVGAALNLGNFNTFLPSLGTKYPGIYHGCYGIGACAGPLFAILMTNNGIQWNYFYYVVLGLTLFNLVFLTLAFKGIDIDIENYTTESDKDESEDTSNQDQKHHDFKAALKDFRTWIGCAFIFFYQGSEVSMGGWVVTFILNYRKGNPTSVGYVSSGFWAGVTIGRFLLTSPLIKYCGNKRSVIVLIGVIMAFDILTWLVPEKIAAGLFASLAGLFIGPIYPMMIVFLTRILPRKIRYCSLVLATAFGSSGGSAIPFLVGILSQISGTFVLHPIFLGCYVLMFLSWIFLPNIERKGAIKHYWQRIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY69</accession>
    <name>A0A1E3NY69_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_95510</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037358.1">
        <property type="nucleotide sequence ID" value="XM_019186497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY69"/>
    <dbReference type="STRING" id="683960.A0A1E3NY69"/>
    <dbReference type="GeneID" id="30203743"/>
    <dbReference type="OrthoDB" id="515692at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032282">
        <property type="entry name" value="HAGH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935">
        <property type="entry name" value="BETA LACTAMASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935:SF94">
        <property type="entry name" value="TENZING NORGAY, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16123">
        <property type="entry name" value="HAGH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="4">
        <location>
            <begin position="6"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxyacylglutathione hydrolase C-terminal" evidence="5">
        <location>
            <begin position="86"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="163" mass="18342" checksum="5AFC227AEC8FA3C5" modified="2017-01-18" version="1">MNGEVLKLGDSIEITAIHTPCHTQDSICYYATDKANPDQKFVFTGDTLFTAGCGRFFEGTGEEMDTALNFKLGKLPKDTLVFPGHEYTKGNVKFAVKLLSNNAIQQLEKFVNENEITTGQFSIGDELKFNPFMRLDDEQITSKLKIDSRSLVMDKLREMKNNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYI3</accession>
    <name>A0A1E3NYI3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SAM-dependent MTase RsmB/NOP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_70831</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036969.1">
        <property type="nucleotide sequence ID" value="XM_019184218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYI3"/>
    <dbReference type="STRING" id="683960.A0A1E3NYI3"/>
    <dbReference type="GeneID" id="30201464"/>
    <dbReference type="OrthoDB" id="10261637at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047046">
        <property type="term" value="F:homoisocitrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009085">
        <property type="term" value="P:lysine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048889">
        <property type="entry name" value="NSUN5_RCM1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF48">
        <property type="entry name" value="HOMOISOCITRATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21153">
        <property type="entry name" value="NSUN5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="9">
        <location>
            <begin position="123"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="223"/>
            <end position="229"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="744" mass="82234" checksum="63E5FBDF3D19A3FE" modified="2017-01-18" version="1">MEEAEKFLTSNQKEGSLQTRIFNASKNKQLKTDPKHVYALVSSTLKYKPFILEIIKKAKLLKEEKQIKESQALLLVHDLLFTRSGRIQMGKTPLKEAILKHKTRLAAELTKLKLKHKVRSLDELIDEDDTPVRWFRANTIKTTKEKVLEELDHLEQVSDIKKIEPGTIYHDEYIPNLFGVHPKERITQLKLYEKGHIIIQDRSSCFPAHILNPGENDHVIDSCAAPGNKTTHLASYIQGPQGSINAFERDVRRSKILDKMVTTAGASKVKINVGDFTASKPSEFEDITGLVVDPSCSGSGIFGRAHEEENPESAYNEERLQKLSSFQFSIVKHALSFPNAKKVVYSTCSIHAQENERVVIDLINDGQVKAAGWRVANRSRVIPLWPRRGLVEEFKGFPNPEQLAGGCIRSLPKVDGGIGFFAFDFVELKAGFEHFKATGTALPEETVEILKNETDGALFGAVSSPTTKVAGYSSPIVALRKKLGLYANVRPVKSVDGTNDRPVDMVIVRENTEDLYVKEEKIFENADGTKTAEAIKRITETATTRIGKIALEIALQRQAQRDLGAASLHKSPILTITHKSNVLSVSDGLFRETVRKVYDQNQSKYSQVNLQEQIVDSMVYRMFREPEIFDVVVAPNLYGDILSDGAAALVGSLGVVPSANVGDNYVIGEPCHGSAPDIAGKGISNPIATIRSTALLLEFLGYNKPAADIYAAVDANLAEGQVKTPDLGGKSTTQQVIDDVVKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYK5</accession>
    <name>A0A1E3NYK5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_64326</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183712.1"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="430" mass="50643" checksum="F70EC7FBCE3C43BA" modified="2017-01-18" version="1">MPGFESNNQRVDERVSRSQWRLKVRKVESWIDNYQTLHNSCTIGNTSTTSKHKSRKLEVALPLGTGEGSILDQSDGDGDDAYTLCEYHRAITGIQWISSKVEPFAKGIFQDIIELIAGHIGDEKKFSQEIAFKIGIYGIVVDEKHKPLLKKYYRDYDLGRILWRVLKDRDLLKKLLPFLTQWCTESVGFVILKPFLRAAEMEYYKTIKDSGDVYDLIVKNYERDVLDAIVPVVMNHEKLSELDAEFLEVELINCKYQFEQHFYLKIILRISEYEITKPIKHLKKLTHDDQYRLKQITLMNAMAKYLSYRDWNERGFQQQVEENFFQQLFKICESMPNVCSILKIFYAFVMNDTSYIHQQSEDEEDWDLMKRCVVKIDDFKVLPKFVEFISKIQLDLPPGVIPKPRPEWERQFISRTSFFGIRASPHELKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYN2</accession>
    <name>A0A1E3NYN2_WICAA</name>
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        <recommendedName>
            <fullName evidence="19">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_58149</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019182817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYN2"/>
    <dbReference type="STRING" id="683960.A0A1E3NYN2"/>
    <dbReference type="GeneID" id="30200063"/>
    <dbReference type="OrthoDB" id="1923159at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16618">
        <property type="entry name" value="mRING-HC-C4C4_CNOT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12438">
        <property type="entry name" value="RRM_CNOT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000257">
        <property type="entry name" value="CCR4-NOT core complex subunit Not4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000006">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034261">
        <property type="entry name" value="CNOT4_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039780">
        <property type="entry name" value="Mot2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039515">
        <property type="entry name" value="NOT4_mRING-HC-C4C4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603:SF0">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14570">
        <property type="entry name" value="zf-RING_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="17"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="121"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="16">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="12">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="83"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="260"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="373"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="554"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="265"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="563"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ57687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="601" mass="67326" checksum="4565B059EDCABE7A" modified="2017-01-18" version="1">MINNNDTFISDEEEELCPLCVEEMDISDKNFKPCPCGYQVCQFCYNNIRQNPELNGRCPACRRPYDDESVEYKIVSQEEWKYEQSKQSRKDRERKQKEKEKKENEQASRKHLAGMRVIQKNLVYVVGLNPPVPPEELHTILRSDKYFGQYGKILKIVINKRNPPPNAPAAIHHHHNNGNAGFGVYVTFSKKEEATRCINAIDGTYIDGRPLKAAYGTTKYCSSYLRGQPCPNPNCMFLHEPGEEADSYTRQDLSTRQGLKMGADQGLPSSSQTSLPLQQQGSSSNISNGVGYKSNSSFTTRHSNNSQFFNHHHQKEQEDVSSTSSHHQPILGSAPLPATASWASNIPQKTSNVSSPVITSSVLANSSAFPTLAESTTLQQQQHQQQQHQARKQKNQSANINDEFNCESSAISFIDETINLLNSFPKFEYQIKSNLVDEETLNSFPPLFAFANIKVNNTSSSFNIKLVESLISKPKVLSPEEQQAILQQQIQPQPILQQPQAQIPQQLTPSQQPQQLNDLAQVYAQQQQQQQQNQPQQQFFSPQATAALLQQQFQATPPPPGLFQQSNSNSNPTPVSANESHVGGTASELLNHLMNGKKIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYW9</accession>
    <name>A0A1E3NYW9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Iron permease FTR1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_34747</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxidase-dependent Fe transporter (OFeT) (TC 9.A.10.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037522.1">
        <property type="nucleotide sequence ID" value="XM_019182226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYW9"/>
    <dbReference type="STRING" id="683960.A0A1E3NYW9"/>
    <dbReference type="GeneID" id="30199472"/>
    <dbReference type="OrthoDB" id="4364at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004923">
        <property type="entry name" value="FTR1/Fip1/EfeU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632">
        <property type="entry name" value="IRON TRANSPORTER FTH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632:SF7">
        <property type="entry name" value="IRON TRANSPORTER FTH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03239">
        <property type="entry name" value="FTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0410">Iron transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="361"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="361"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="419" mass="47550" checksum="3043CC4288A2A680" modified="2017-01-18" version="1">MSGDLFSLKVFFILLRETFESAIVISILLSFLKQNFTKQVIYRHLQAQVWLGGFFGLFICLLIGSVFVALFYIIGENYWSKYERNWEAIFSVISSLIISIMGLSLLRINQMQKKWQVKLGKNMHGIIVNSETDAISHRKKLKGLKGKLKRLTEKNSLMILPMVTLLREGLEAIFVISGVSASEPASSLPLSIISALTLGSLIGVFLYKGGNKMRLQFFLIFSTCFLYLVSAGLMSRGVWFFELEQYVQKCHGQDMTEVGNGPGSYDVANTIWHVNCCSGINDGGWMLLNALVGWTNTATYGSVISYNLYWLFIVILLNVKLYQERHGYIPFIPIKWQLKKISKRYEIIKFNLENNDNYKRPEVELQSRDNSRNISSNGYNSVRGESVDNLQNTRLDVINDDNSDATTSLLASSQHTASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYY0</accession>
    <name>A0A1E3NYY0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CUE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_34580</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037586.1">
        <property type="nucleotide sequence ID" value="XM_019182205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYY0"/>
    <dbReference type="STRING" id="683960.A0A1E3NYY0"/>
    <dbReference type="GeneID" id="30199451"/>
    <dbReference type="OrthoDB" id="5577209at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14373">
        <property type="entry name" value="CUE_Cue3p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041808">
        <property type="entry name" value="Cue3_CUE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUE" evidence="2">
        <location>
            <begin position="308"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="493"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="557"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="577" mass="66389" checksum="436307BF1A93995F" modified="2017-01-18" version="1">MSDEEDNVYVELPVAQFPPFKLRSSMVDKDPVIWVHEIEVYITYIQTLLQLKRQLSQRSEQQLCLFLKSYLHEIAEEQGQILSLGLINVQITQNLDTLRSWILELIKQLGVLNLKLNGAALWHFAKVYAAKNATIVKGIIDGTYKPKDKKTINSISLVHKHLEHLITNGKFEKHDLETLAVLLQKSAKVQTESKATKEPFLKQTKAKKGTSFGETFVTLSWIDMLEKLHANGEGKFSKLVRNLAVLSLVSVPIAKVASIGTELGINTYSSLRLYPLFGGIIASESFKELNPGIEQRFPFLIKQPKSDVNEADVKTLTELFPDLSISHTAKLLKKHNNNVEEVTMLLLEHPEAAIEKEEDKSRHVMENKTKNVKLLSNRSKLQKESELHVPDELKNKTLTSALRLMYEADEDEHDDTYDEAEAVTPDEKGKFDKAEKILWEAYKRDPNVFAKPNRRWNERQQLVQTLHWSHEQIEGWARMLEKEPRRVRILEQKYQFRGNKPQRRTEEDEEIAEERRPPPSSSTDSHNDQNKKKPNQTVKANPANVKRQNAKNEKNKSSKANHNRKSGHDKKMARELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ21</accession>
    <name>A0A1E3NZ21_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alcohol acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_85905</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037044.1">
        <property type="nucleotide sequence ID" value="XM_019185968.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ21"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ21"/>
    <dbReference type="GeneID" id="30203214"/>
    <dbReference type="OrthoDB" id="2150604at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.30">
        <property type="entry name" value="Nonribosomal peptide synthetase, condensation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052058">
        <property type="entry name" value="Alcohol_O-acetyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010828">
        <property type="entry name" value="Atf2/Sli1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037:SF1">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07247">
        <property type="entry name" value="AATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="437" mass="50431" checksum="02F3D0C3F8A41533" modified="2017-01-18" version="1">MTRQIEGLEKRNYYRNLLSTYSCFIVGGRLNIKFENKDVLREAVRQLFESRKQLRLNVFKDDQNNTLVEKEIENGWDSKEVVEFMNTSEDYSITEILNDLHEKTFKLEVQKPMWKIVVVNQEWIMVLCDHTLYDGTTAALILEDLLTLLNKEGLPVDKDLNLSKFVELTTPSYTYTTCKILEHFSPNFMKPFFKSMFSYGSKYEAFDGPGWVENAGTKEKTFRTLKQLISIDMEDFMKLKKLIDLHGVKFTAFWVYLSIISLSKIKNHDNIKVSVPCNLRSLLPSNFENHYGLFVSAVFLNLKPVATENIDWDYVKFINSTITRNELIKGASLIGMLKYVDPKEGVSKEATSPRKTTYEISNLGLRKIPTLDNQVYSFDEFIFSQPNSLTGTQLTNSVISSSKKVNIVVDGTPETKSFYHQYVQILNSQLQEILSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ65</accession>
    <name>A0A1E3NZ65_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_96426</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037686.1">
        <property type="nucleotide sequence ID" value="XM_019186515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ65"/>
    <dbReference type="GeneID" id="30203761"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="57" mass="6831" checksum="A1E6CC5CEC14B852" modified="2017-01-18" version="1">MLRASEYSGRYYGHLGLTKLDTDNVKYIMSSWSSLKVNSWIWYLVKKKDRQLNTRQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZD1</accession>
    <name>A0A1E3NZD1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_34750</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037164.1">
        <property type="nucleotide sequence ID" value="XM_019182227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZD1"/>
    <dbReference type="STRING" id="683960.A0A1E3NZD1"/>
    <dbReference type="GeneID" id="30199473"/>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="11">
        <location>
            <begin position="112"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ57957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="336" mass="39467" checksum="82D6959DDD6B7C74" modified="2017-01-18" version="1">MSNSKLTFQRLARKSIPIPIFLGMVYLSWAYSYYICWNEVYKHHSKATGIGLIIGNIAFILLIYFIWCQLLIMNPGVQPKMPVFKLLEDGDDASYRWVHPPEYFMCDAHGYPTWCNSCQSIKVDRSHHSSDLNRCVARMDHYCTFLGTTIGKRNYRLFLQFCFWYLCYFIYIGVSMACFTRSYKIRHGSVNANTIILYVASLGWIILIFALFSSHIYYIFKNRTSIDDMAAKRNRRAKSLNEEFINISHEEKRYVLRVSSMKDGIWNKGFAKNWVEIMGNNMIFWIIPWRSPLEHLGDESSNDYYSIIGDYKEEISESFKKSLVKRIQADDYISSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZM9</accession>
    <name>A0A1E3NZM9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">beta-glucosidase</fullName>
            <ecNumber evidence="3 6">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_68987</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Glycan metabolism; cellulose degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="3 6"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037762.1">
        <property type="nucleotide sequence ID" value="XM_019184150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZM9"/>
    <dbReference type="STRING" id="683960.A0A1E3NZM9"/>
    <dbReference type="GeneID" id="30201396"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="UniPathway" id="UPA00696"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.300:FF:000006">
        <property type="entry name" value="Beta-glucosidase H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000495">
        <property type="entry name" value="Periplasmic beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050288">
        <property type="entry name" value="Cellulose_deg_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019800">
        <property type="entry name" value="Glyco_hydro_3_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715:SF27">
        <property type="entry name" value="BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07691">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00775">
        <property type="entry name" value="GLYCOSYL_HYDROL_F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA14" evidence="7">
        <location>
            <begin position="395"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="836" mass="92049" checksum="F0702DF2DBF2D234" modified="2017-01-18" version="1">MAEKFDVEDILEKLTTNEKIGLLSGGDYWSTYPIHRLNVPSIRTSDGPNGIRGQHHFNSTPTAAIPVGTALASTWNTELLHQVGEFLDLEARAKGVHVLLAPTVNIQRIPNGGRGFESFSEDPLLSGKLAASYINGFQSKGLAATIKHFVGNDQENERFSSDSVISQRALREIYLKPFEIAIEESNPKALMTSYNKVNGVHVSEDEYLLKTILRGEWNWNGLVMSDWTGTYSTSDALNAGLDLEMPGPGRWRGERVSHSLLSNKILESTLDERALNVLNLVKLVKDSGVPENAPEAERNAPEDREFLRKVAGEGIVLLKNDQNVLPFQKKRTLVIGPNALYAAFSGGGSALATPYYAVSPIQGVKEKIGDENVVFNIGAYNHKFLPSLKHELYHEGVNGTLFKIYNEPPEDTSRVPFDTLVLDDVNYFRMNDYVHPKLKSEKFYAELVSSFIPAFDGVYEFGLAVFGTAKLFIEDELVVDNATNQELGESFYGLGTPEIKGKFKVTSGKHYKLVVQFGSGATSDVAKGANKTFYGGGGLRFGGALVIDPEKEIEKAAANAKLFDQVVIITGLNKDWESEGGDREDLKLPGYSDKLIEAVLDANSNSVVLIQAGTPVELPWVSKASTVVYTSYGGDETGNAIADVLYGDINPSGKLPLSFPTRFEDTPSLINNTTNNGKVLYGEDIYVGYRFYEKVDKAVVFPFGHGLSYTRFELSGLRLNIVEDKLSVSLTIKNVGSYDGAEVVQLYVGSKKLNIQKPVKELKAFTKVELKQNVKSDISFKLNLGKSTAFFDELKNKWVSEKGEYFVYVGTSSSDLKALKADFSIEKTTSWLVPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZR4</accession>
    <name>A0A1E3NZR4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_93042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037802.1">
        <property type="nucleotide sequence ID" value="XM_019186394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZR4"/>
    <dbReference type="STRING" id="683960.A0A1E3NZR4"/>
    <dbReference type="GeneID" id="30203640"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="12779" checksum="93652316A85C317D" modified="2017-01-18" version="1" fragment="single">MECKFDFTTDQLNNSDLKNFKMICDNCNHRRTTCIIPRNSNHCTECTLRDLQCKFSDSVNNDAKIRTNIPIRDYDNYQGPTIMKKTLSLQGPKSSKLLGPTSIVEKNLLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZR9</accession>
    <name>A0A1E3NZR9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">asparaginase</fullName>
            <ecNumber evidence="2">3.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_45542</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-asparagine + H2O = L-aspartate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="EC" id="3.5.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the asparaginase 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.1" evidence="2"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037324.1">
        <property type="nucleotide sequence ID" value="XM_019182588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZR9"/>
    <dbReference type="STRING" id="683960.A0A1E3NZR9"/>
    <dbReference type="GeneID" id="30199834"/>
    <dbReference type="OrthoDB" id="542841at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006530">
        <property type="term" value="P:L-asparagine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08964">
        <property type="entry name" value="L-asparaginase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1170:FF:000001">
        <property type="entry name" value="L-asparaginase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004550">
        <property type="entry name" value="AsnASE_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00520">
        <property type="entry name" value="asnASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500176">
        <property type="entry name" value="L_ASNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133602" description="asparaginase" evidence="10">
        <location>
            <begin position="22"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="L-asparaginase N-terminal" evidence="11">
        <location>
            <begin position="41"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="12">
        <location>
            <begin position="255"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="active site" description="O-isoaspartyl threonine intermediate" evidence="5">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="128"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ58117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="368" mass="39361" checksum="FA0B5BC46FE14D0B" modified="2017-01-18" version="1">MLFSKPILASILLSLITSSQAAPFSKREDTSALKNASLPTVKIFGTGGTIASRGTSGSQTAGYSLGLTVADLVDSIPDLANAANIEYIQVSNVGSPSVNYTHLIPLHHNISESLDSDEIDGAVVTHGTDTLEETAFFLDLTIKSEKPVCIVGAMRPATALSADGSMNLYQAVSIAGSEEAKGRGALIPFNDRISSAFWATKTNANTLDTFKAVEQGFLGTFLNNDVEFFYPPVRPIGYHYFDISNVTDASDIPEVAILYSHQGLDPELVRFVVEERGAKGIILAGTGAGGWTSTGYPVLQEMIEKYNIPVVRSRRTMDGSVPNTGSRSQYISSGYLNPQKARIMLQLCLHAGYDISQIKTVFSGVYGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZT4</accession>
    <name>A0A1E3NZT4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Histidinol-phosphatase</fullName>
            <shortName evidence="8">HolPase</shortName>
            <ecNumber evidence="3 8">3.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_80635</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>L-histidinol phosphate + H2O = L-histidinol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14465"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="3.1.3.15"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 8/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the PHP hydrolase family. HisK subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.15" evidence="3 8"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037707.1">
        <property type="nucleotide sequence ID" value="XM_019185101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZT4"/>
    <dbReference type="STRING" id="683960.A0A1E3NZT4"/>
    <dbReference type="GeneID" id="30202347"/>
    <dbReference type="OrthoDB" id="5957391at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00013"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004401">
        <property type="term" value="F:histidinol-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12110">
        <property type="entry name" value="PHP_HisPPase_Hisj_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010140">
        <property type="entry name" value="Histidinol_P_phosphatase_HisJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01856">
        <property type="entry name" value="hisJ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21039">
        <property type="entry name" value="HISTIDINOL PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21039:SF0">
        <property type="entry name" value="HISTIDINOL-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHP" evidence="9">
        <location>
            <begin position="4"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="319" mass="36981" checksum="824FFA9CF748CB7D" modified="2017-01-18" version="1">MHSHHSHSGDYVAHATDTLEGIADRAIEMGFETFCLTEHMPRIDSKFLYPEEIEQNYGIETLETNFDKFYHHARRLQKEKIKSGCKTHFLVGLEVEGIDEAHIEFAAQILEKYDIDMTVGSVHFVKQIPIDFNRENWELAKDRCGGIRGLFKEYFELQYKVLTKLKPLVVGHFDLIRLFSDDDDLDETTGLTLGQIELVQDWPEIWDLITRNLIFINSYGGIIEFNSAAIRKGWKTPYPKMDICQAVIQYCGSRFCLSDDSHAIAQVGLNYHKVKEYVNELKLKKLLYLDFDSASGKIEIKEKPVDDFNSSEFWSQYSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZV1</accession>
    <name>A0A1E3NZV1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1776-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_80654</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037727.1">
        <property type="nucleotide sequence ID" value="XM_019185104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZV1"/>
    <dbReference type="STRING" id="683960.A0A1E3NZV1"/>
    <dbReference type="GeneID" id="30202350"/>
    <dbReference type="OrthoDB" id="5308060at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013952">
        <property type="entry name" value="DUF1776_fun"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08643">
        <property type="entry name" value="DUF1776"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="396" mass="44726" checksum="852146BE905CBC6C" modified="2017-01-18" version="1">MDPVDKTFDTVYYLGKKAQNTFSSAIGTVSDTTHTISDKVISTYNNNFNYGSNNTTQLTDSANIPSSGFWDLNGVRGFYHRNKAFSTIIGVTTLGITGYYLQMVVRSQVTNTSKSVTRRADRLPNGARKDVVLIVGSVTEPLTRYIANDLENRGFIVYITSTNSKSDLKFFNNESIQDIKSLIVSNEVDNDEFNTEQLRKFDYLLSSNHIPFQGAEPNKLNLTGIIFIPDLYFPSGKFHMIPNSTWSHNVQNKMLLPLNLLTNGLIGIAEKFDSNLIFITPTLSSSVQLTYHSVENLTSIFLQTLSQNLAQDYEGLNITNIKLGSISINSNNSSKKNFGIKGIPLKPLHYKIFDLLYNDDNNSVEYVGTWARFLDKYGFLIPRWLIRNYFDNYFGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZX6</accession>
    <name>A0A1E3NZX6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_16902</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037449.1">
        <property type="nucleotide sequence ID" value="XM_019180717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZX6"/>
    <dbReference type="STRING" id="683960.A0A1E3NZX6"/>
    <dbReference type="GeneID" id="30197963"/>
    <dbReference type="OrthoDB" id="446113at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061158">
        <property type="term" value="P:3'-UTR-mediated mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12611">
        <property type="entry name" value="RRM1_NGR1_NAM8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050825">
        <property type="entry name" value="RBM42_RBP45_47-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640:SF10">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="15"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="114"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="236"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="194"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="196"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="308" mass="33451" checksum="DEE24676F8F5D06C" modified="2017-01-18" version="1" fragment="single">NPPTATAQTSSSPPKTLWMGDLDPWSDEDAIANLWSSLGKRVLVKLIRAKKGTPAATLNTGHAGYCFIEFETYDDAKSALSLNGSQIPNTNRLFRLNWASGATLSSPIPQSPEFSLFVGDLSPSTTEAHLLALFQTHFKSVKTVRVMTDPITGTSRCFGFVRFSDEEERRRALTEMQGVWCAGRPLRVALATPRNQANSQNDQQQQLQQQIPGQGQLDQAPSANSQSVPYTDPNNTTVFIGGLASGIPEQTLAALFQPFGNITHVKIPPGKGCGFIRFDKREDAEAAIAGMQGFQIGGSRVRLSWGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P105</accession>
    <name>A0A1E3P105_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCAAT-binding factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_93046</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037809.1">
        <property type="nucleotide sequence ID" value="XM_019186397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P105"/>
    <dbReference type="STRING" id="683960.A0A1E3P105"/>
    <dbReference type="GeneID" id="30203643"/>
    <dbReference type="OrthoDB" id="10263185at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027193">
        <property type="entry name" value="Noc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455:SF0">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCAAT-binding factor" evidence="2">
        <location>
            <begin position="214"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="421" mass="49279" checksum="55F681ED23A8679F" modified="2017-01-18" version="1">MFKFNLLFIIENVIFDCSLIIDNLDIYLKLVKLESVHFATSSKEGEKNPFFPTKTFKNLVTSILKSQNGEVLNDGTSSNIVVQEFVNSYFKKYQDFQFYFINELNTEELNDVSNEQTFSKFLTIMKEKLTLFESDDEKIGKTFVTNLPSIVNNQVHFKSTFETKWLYFLNRELNNNQYKTILLILHKRIIPFFSTPTKLMDFLTDSYEVGGIISILSLNGLFELIKKYNLDYPNFYEKLYSLFDQDLFHMKYRSRFLRLTDVFLSSTHLPSALIASFLKKMARLSLNSSPSAIVSIIPFTYNMLKKHPTCMILLHNTSASDDYTDPFDDSEKDPLKTKAIESSLWELETLQTHYHPNVATLAKIFSQPFRKQSYNMEDFLDWSYNSLLESENTRRMKNEVALEHDKFDSLLGDGYISGWKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P116</accession>
    <name>A0A1E3P116_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_79702</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FKBP-type PPIase family. FKBP1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038383.1">
        <property type="nucleotide sequence ID" value="XM_019184938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P116"/>
    <dbReference type="STRING" id="683960.A0A1E3P116"/>
    <dbReference type="GeneID" id="30202184"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043189">
        <property type="term" value="C:H4/H2A histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000025">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012423">
        <property type="entry name" value="Eaf7/MRGBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050689">
        <property type="entry name" value="FKBP-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10516:SF443">
        <property type="entry name" value="FK506-BINDING PROTEIN 59-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10516">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07904">
        <property type="entry name" value="Eaf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="7">
        <location>
            <begin position="253"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="70"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="136"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="341" mass="38549" checksum="4F260B695CABCAC2" modified="2017-01-18" version="1">MAMVMREWETTEEITLLRSLCRFKPVGQNKHFHMISILQELNLGRNDEKIIKAVDVWEKLKSLYDLEGLDKLEDSDDEDDDDNDDEEEEEEKEDPVKIFDLPWSQFGEIMEAKAKDGRKSETTTPEPSANDLSIKDEEEEEEEEQEEGEEEEIEEQEGDEEEGENGGEDEGNGSVKDETEDEETNEEASEDEEDEVNSIHTSEAINEMLATRTKLGLFTRQAPRFFSTTIPRMSDLKIERVSPGDGVNFPKQGDLVTIHYVGTLENGKKFDSSRDRGKPFQTNIGVGQVIKGWDEGIPQLSLGEKAILTIPGSKAYGDRGFPGLIPPNATLIFDVELLKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1G5</accession>
    <name>A0A1E3P1G5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_63534</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038241.1">
        <property type="nucleotide sequence ID" value="XM_019183567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1G5"/>
    <dbReference type="STRING" id="683960.A0A1E3P1G5"/>
    <dbReference type="GeneID" id="30200813"/>
    <dbReference type="OrthoDB" id="5578329at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007727">
        <property type="entry name" value="Spo12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05032">
        <property type="entry name" value="Spo12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="77" mass="8507" checksum="6973EFAB6BA5A91F" modified="2017-01-18" version="1">MSSSANIQPLHRNPAPSQGNLQRKLFQRNFGGAQGAFASPTDNMLSPCSQKLNAHKNRFFANTKATKLNFSNLEDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1S8</accession>
    <name>A0A1E3P1S8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 14">Acyl-coenzyme A oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">WICANDRAFT_69570</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2,3-saturated acyl-CoA + O2 = a (2E)-enoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:38959"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="ChEBI" id="CHEBI:65111"/>
            <dbReference type="EC" id="1.3.3.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; peroxisomal fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Heteropentamer composed of five different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 14">Belongs to the acyl-CoA oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038407.1">
        <property type="nucleotide sequence ID" value="XM_019184172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1S8"/>
    <dbReference type="STRING" id="683960.A0A1E3P1S8"/>
    <dbReference type="GeneID" id="30201418"/>
    <dbReference type="OrthoDB" id="538336at2759"/>
    <dbReference type="UniPathway" id="UPA00661"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003997">
        <property type="term" value="F:acyl-CoA oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033540">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA oxidase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000018">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000013">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000015">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000003">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055060">
        <property type="entry name" value="ACOX_C_alpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029320">
        <property type="entry name" value="Acyl-CoA_ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012258">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002655">
        <property type="entry name" value="Acyl-CoA_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909:SF352">
        <property type="entry name" value="ACYL-COENZYME A OXIDASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909">
        <property type="entry name" value="ELECTRON TRANSPORT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01756">
        <property type="entry name" value="ACOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22924">
        <property type="entry name" value="ACOX_C_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14749">
        <property type="entry name" value="Acyl-CoA_ox_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000168">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14 16" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 14" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-coenzyme A oxidase N-terminal" evidence="19">
        <location>
            <begin position="21"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="18">
        <location>
            <begin position="142"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-alpha1" evidence="20">
        <location>
            <begin position="281"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-terminal" evidence="17">
        <location>
            <begin position="485"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="433"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00063271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR000168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF14749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF22924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ59200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="682" mass="77279" checksum="0B7DC52BADCDCBA0" modified="2017-01-18" version="1">MSESEDLIASRAKERGAASFNVRDLTYFLDNGKQNTERNESIMLQFERDPLFNIKTFNLDLKGQREVSFQRITKLAAYNNNDDEQGIRARMNILSISDPGTYTRVGVHYGLFLQTLRALGTPQQFQYWVSKGAGSLSKFFGCFGMTELAHGSNVAGLQTVANFNPSDDTFTINTPNLGATKWWIGGAAHSATHCVVYARLVVKGKDYGVKNFVVQLRNDNGELLPGVAIGDIGKKMGRDGIDNGWIQFTNVKIPRFNMLSKYAQVDSRGKVTVPEFQQLGYSALVMGRVSMVVDSYNIGKKFITIALRYATVRRQFGKDSDGLEKKIIDYTHHRRRLFPFLATIYAFNSSALEITRVQDETLKLLAGGDKNVKDVIPNLKDLFSLSAGLKAFATWETAKLIDEARQSCGGHGYSSYNGFAQGYNDWVVQCTWEGDNNVLSLSCGRALIQNYLAVEKGERVGDFTKYLKVEDNDDFVLRTQDLEYIEKIINAWEIVSKRLVQSSTAKFKQFRKETNGDLELAFEKVASNRFNIAKIHTKLVILKTFHQRVSHAPENLKSILSLLLQLYALHITSENISQFYSFNIVKGSDFLENLNYKIDEINELLRPNIIGLTDAFNLSDFMIGSQIGKKNGDIYENYFKFVTDNNTEIKPHYYDSLMKPFFERSLKPTDNEEVQENEEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1U2</accession>
    <name>A0A1E3P1U2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GDS1 winged helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_32139</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038520.1">
        <property type="nucleotide sequence ID" value="XM_019181920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1U2"/>
    <dbReference type="GeneID" id="30199166"/>
    <dbReference type="OrthoDB" id="4090479at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057511">
        <property type="entry name" value="WH_GDS1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25318">
        <property type="entry name" value="WHD_GDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GDS1 winged helix" evidence="2">
        <location>
            <begin position="124"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="435" mass="47101" checksum="7AA2B96BBEA4E7C2" modified="2017-01-18" version="1">MALANRRPVAFPMGDSTATTSPAFKPRRLSLNSDNSKPNNSALSESIKEMTPEKSSSPEEFKDVSPTSTVATSVSSSSSKPPKEKKEKAEKGEKKHSKPSSRAPIATGISTTIPVTGERPKPTQHPSLDDDVLFAIFEILHERDEDRKGMTVKQICDILVEKHPKMATLSTKTSNLVSAKLNAYVKRVEKGEKALIYSLSREWADASPKRMVYVYRGLLAPDYYVHALAAIESQKSQEQSIYSPFGSLNDNDFSNTGSEMKRRATAFDLGVTKHTFADLQLDLTLPQLSIPYSAAPVTASLGLGSTLKSPSSVSKSYESPIISEYDFEEMDQFDEDDDEDGGHVKITLRNNKRSKSMSYLSSKKVRTLTAAAAAPRFSKVAVSPSPSAAAAAAALHAAALEGLSPAGSAVRDGFLTQDIETPEDISLAELDSLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1U8</accession>
    <name>A0A1E3P1U8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glycosyltransferase family 15 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_19018</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038638.1">
        <property type="nucleotide sequence ID" value="XM_019180813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1U8"/>
    <dbReference type="GeneID" id="30198059"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF6">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="344" mass="41787" checksum="3D70029BB85E6A32" modified="2017-01-18" version="1" fragment="single">NDEINPNYEKENATFFSLVRNEELYLMLQSITHVEERFNKKYHYPWIFANDAEFTDTFKNEVSNLVSGNVTFVTIPKEYWSYPEFIDQKRAADVRKQMQKDNIKYGGSESYRHMCRFNSGFFYKLKAFEGIKYYWRVEPDIKFTCDLNYDYFKFMRENNKKYAFTMTLHELHNTVYGLFEATKEFFHDQNPSYIAKENTIDFITLDNEETFNMCHYWSNFELGDLDFLRSKEYEEYFQYLDSKGGFFYERWGDAPIHTFAVSYLLNKNEVHYFDNTGYYHVPHTQCPRRQEVREELHCVCSPNYDYNWGNRDSCLAYWYEARNEPRPEHAPRKTYSTHKPKENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1Y5</accession>
    <name>A0A1E3P1Y5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex component Sec8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_15875</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SEC8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038678.1">
        <property type="nucleotide sequence ID" value="XM_019180662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1Y5"/>
    <dbReference type="STRING" id="683960.A0A1E3P1Y5"/>
    <dbReference type="GeneID" id="30197908"/>
    <dbReference type="OrthoDB" id="272977at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039682">
        <property type="entry name" value="Sec8/EXOC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007191">
        <property type="entry name" value="Sec8_exocyst_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048630">
        <property type="entry name" value="Sec8_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20652">
        <property type="entry name" value="Sec8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04048">
        <property type="entry name" value="Sec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec8 N-terminal" evidence="6">
        <location>
            <begin position="36"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec8 middle helical bundle" evidence="7">
        <location>
            <begin position="320"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="692" mass="79715" checksum="1E799A60CF901C8C" modified="2017-01-18" version="1" fragment="single">MSSLNVPSATRRTRALSVNKTDEAQRSRMEQSLDNLQYTLSEVESHWSKTIGDNANPLDIALPLLDNTSVGLAHRHREFLDLKDRISNGLRQAVNEHFQAFNDSVGSYRSVVQNIANSQHNLSKVKDTINSTTNGLNEKGDTMNTLNENLKTYNDMIEVLNVIEYLKKVPNELEDAISEKNYSKAQFLLQSVSSKSKTYNLWELPALSNLESYFQLQTQQLFEILIEEIHNIIYSKKLFNTFNSANEVGKDSDISDYSNIEKYLMNAIDVDILEAASVNHSNIENFLSFLINSSSKNKEDPSQLDNENDMANMVALDDVNPFNQLLHLFTIIYRLDKLPTAIEIIIQRNANEINQLVNRITEDTKLRHPKIIKYLNSNIKSKDLSRGLGIDDSLNDDNFKTMIVRDLFWNFFRKLLFLIQSVRVILEIIKSLENDSFETVSVDVSNLNGTSPHGSNDSLLTLIKLWHSLKFEVKNFITSYITNNDLSLSSKLTNTSSIINQKDKMNNNALFQFYKVNYDEDHSNQLKLVLQDLFPGFINSNDMSKIDSPYIEDTKFLKQTRIIPANIFHMRVILEPFLVFIQGSKLLLNEQDNDEPIKFFNGFMNNDFLPLLEESFIGFYIDEVEELDPYEIYDDVTEKSTNVPHSQSSGVKIFKIFIEFKRFFSDICFVLNTSLQFRKEFSGIIFKILNKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2B0</accession>
    <name>A0A1E3P2B0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alcohol dehydrogenase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_91352</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038823.1">
        <property type="nucleotide sequence ID" value="XM_019186290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2B0"/>
    <dbReference type="STRING" id="683960.A0A1E3P2B0"/>
    <dbReference type="GeneID" id="30203536"/>
    <dbReference type="OrthoDB" id="5363962at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000721">
        <property type="term" value="F:(R,R)-butanediol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034079">
        <property type="term" value="P:butanediol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161:SF23">
        <property type="entry name" value="(R,R)-BUTANEDIOL DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="6">
        <location>
            <begin position="112"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="255" mass="27238" checksum="82958B7FDA61A5EF" modified="2017-01-18" version="1" fragment="single">MDVGDKVAIEPTVFCSDSPRFQNTNLSKDYSCAPCSKGLTNICDYAGICGLGAQDGGLAEKFVTGARHAIVIPESIPLEVGALVQPIAVSWHAVRISGFKEDSSALVLGGGAVGLAAILSCFGHKASMVVCSEPSKRRRESAEKLGAHVFDPSSVDGDPIKELMDLSPKGHGFDYCFDCSGLEITLHTAIRAATTNGTIVNVAIWGSDSINFYPMEITKTEKRLMGSMCYTREDFEGVLDAFEKGNIDVDKTKTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2C0</accession>
    <name>A0A1E3P2C0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Geranylgeranyl transferase type-2 subunit beta</fullName>
            <ecNumber evidence="3 11">2.5.1.60</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_104634</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.60" evidence="3 11"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038752.1">
        <property type="nucleotide sequence ID" value="XM_019180422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2C0"/>
    <dbReference type="STRING" id="683960.A0A1E3P2C0"/>
    <dbReference type="GeneID" id="30197668"/>
    <dbReference type="OrthoDB" id="5428259at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170069">
        <property type="term" value="C:geranylgeranyltransferase-III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02894">
        <property type="entry name" value="GGTase-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000012">
        <property type="entry name" value="Geranylgeranyl transferase type-2 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026873">
        <property type="entry name" value="Ptb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF11">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="12">
        <location>
            <begin position="3"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="318" mass="35487" checksum="717D1031E9381009" modified="2017-01-18" version="1">MALYKEKHIEYIVSLDTHVKDYQYWLSEHLRLNGTYWGLTALSLLDSKDSFKKQDVIDFVLSCYNEDGGFGAFKNHDSHLLSTLSALQILLIYDSLDVLTDQGIEKTVKFITDLQLPNGAFQGDRFGEVDTRFVYTAIQSLAILGRLSSKVVDKAVEFILSCANFDGGFGLAPGAESHSAQVFTCLGTLAIADKLDLVDVELTGWWLSERQVENGGLNGRPSKLPDVCYSWWVLSSLALINKLGYIDFAKLREFILSCQDEVTGGISDRPGNQVDVFHTIFGLAGLSLMGYENLVPVDPMYCLPVEVGKKIKKWPYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2G0</accession>
    <name>A0A1E3P2G0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">W2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_83701</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038792.1">
        <property type="nucleotide sequence ID" value="XM_019185583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2G0"/>
    <dbReference type="STRING" id="683960.A0A1E3P2G0"/>
    <dbReference type="GeneID" id="30202829"/>
    <dbReference type="OrthoDB" id="10250831at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071074">
        <property type="term" value="F:eukaryotic initiation factor eIF2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045947">
        <property type="term" value="P:negative regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11561">
        <property type="entry name" value="W2_eIF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000031">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.350:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF7">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02020">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00515">
        <property type="entry name" value="eIF5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="W2" evidence="7">
        <location>
            <begin position="242"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="406" mass="45170" checksum="2B540D7054F48E6D" modified="2017-01-18" version="1">MSFINICRDNTDPFYRYKMPPIQSKIEGRGNGIKTAVVNTAEVARALGRPPAYLIKFFGFELGAQTSINESNDRYLVNGVHDAPKLQDSLDGFINKFVLCGSCKNPETDIIITKDEDLIRDCKACGQRTRIDPRLKLSTYILKNPPESAKSKKKKAATASANVVGGGMSITDIASGKQRSSAPVEDDQAEDSDDDALTRKINAEASALPQTEVADDEWAVDMSEEAVRKRAQELEALTLENGSGGDSKFDEFGEWLLEFGDDKSNLPNDVEIYKKAFELEISEDVHTVQVLAQVLFDKNIVKQIPTHKGLLTKIITSKNHEKAFLGGLERFLGLDNPELLPQISKILLTIYENDIVNEETIKDWGSKVSKKYVSKEISKKVKKAAKPFIEWLDEAEEESSDEEESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2L1</accession>
    <name>A0A1E3P2L1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">mRNA 3'-end-processing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_83802</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the cleavage factor I (CF I) involved in pre-mRNA 3'-end processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the CPSF4/YTH1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038936.1">
        <property type="nucleotide sequence ID" value="XM_019185600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2L1"/>
    <dbReference type="STRING" id="683960.A0A1E3P2L1"/>
    <dbReference type="GeneID" id="30202846"/>
    <dbReference type="OrthoDB" id="1914176at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="cleavage and polyadenylation specificity factor subunit 4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CPSF4/Yth1"/>
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        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
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        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23102:SF24">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="28"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="86"/>
            <end position="114"/>
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    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="115"/>
            <end position="142"/>
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    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="144"/>
            <end position="166"/>
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            <begin position="28"/>
            <end position="56"/>
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            <begin position="58"/>
            <end position="85"/>
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            <begin position="115"/>
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            <begin position="144"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00008907"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ59729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="212" mass="24957" checksum="8BAC076CE5042DEB" modified="2017-01-18" version="1">MSVIHPNSSNYPFKFEPFLRTEYNFGLDPDRPVCQFWNGHGGCPNGDQCPNKHVLPMYSNKIVCKHWLRGLCKKGDHCEFLHEYNLRKMPECLFYSKNGFCTQTPECQYLHIDPQSKIPECLNYSRGFCPEGPNCKNRHIKKIICQNYLTGFCPDGPDCDLAHPQFELLPDKLRIRPDRAVEIQLEKEAKEREEKEREELERFQKKVVDPEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2U0</accession>
    <name>A0A1E3P2U0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphopyruvate hydratase</fullName>
            <ecNumber evidence="3">4.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_68381</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enolase family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ59799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019184116.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3P2U0"/>
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    <dbReference type="OrthoDB" id="1739814at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Enolase 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Enolase"/>
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        <property type="entry name" value="Enolase-like_C_sf"/>
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        <property type="entry name" value="Enolase-like_N"/>
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    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="12">
        <location>
            <begin position="4"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="11">
        <location>
            <begin position="149"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="383"/>
            <end position="386"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="447" mass="48905" checksum="751AAE97E2DE4214" modified="2017-01-18" version="1">MTTIERIHARYVYDSRGNPTVEVDLTTNLGTFRSIVPSGASTGSNEAVELKDGDKSKWEGKGVLQAVENVNEILGPELIKSEISVTKQKQVDDFLKKLDGTANKIKLGANAILGVSMAVCKAGAAELKLPLYEHIAQLAGDEAAIKNKKYVLPVPFLNVLNGGVHAGGKLAFQEFMIAPIGASSFREGLRYATEVYHTLKKLAQSKYGQGAGNVGDEGGVAPDVDTAEEALDLIYEAIKKCGYEGKVCIGMDAASSEFHKVAKQYDLDFKSPEHNKILSGEQLGELYVELLQKYPIVLLEDPFAEDDWDSWKHFYNVTYKKLDQNVEIVTDDLTVTNTKLLQNAIQERVGNSLLLKVNQIGTVSESIDAANMAFKNDWSVFVSHRSGETEDTFIADLTVGLKAGHLKSGAPARSERLAKYNQLLRIEEQIGDDGVYAGRDFGRAKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P307</accession>
    <name>A0A1E3P307_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_78336</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038905.1">
        <property type="nucleotide sequence ID" value="XM_019184706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P307"/>
    <dbReference type="GeneID" id="30201952"/>
    <dbReference type="OrthoDB" id="3979958at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="109" mass="11959" checksum="4795A3563C139382" modified="2017-01-18" version="1">MSGIFDKYLNMINGSKHTHGQMNTKINAGTQRHSYVNGHDKYVPYERRKSSVSSIGSDAEGASRNSSVSSQQGITESMNPSNNPFQNPGVVTDAQVKKVLSEPNNRVNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P317</accession>
    <name>A0A1E3P317_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MRH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_15434</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039088.1">
        <property type="nucleotide sequence ID" value="XM_019180644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P317"/>
    <dbReference type="STRING" id="683960.A0A1E3P317"/>
    <dbReference type="GeneID" id="30197890"/>
    <dbReference type="OrthoDB" id="4504960at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028927">
        <property type="entry name" value="Man-6-P_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071:SF0">
        <property type="entry name" value="MANNOSE 6-PHOSPHATE RECEPTOR-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02157">
        <property type="entry name" value="Man-6-P_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="10">
        <location>
            <begin position="6"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="199" mass="22140" checksum="DD5A9F9AF53FF142" modified="2017-01-18" version="1" fragment="single">EEPKLNPCSAIHPLTNGFFDLSELSSINKENTVAFSAKGYDYNKNFTLSICSSPLKPNIDVNNNYNGHFAKYHINSSEVGGFYTDEAGDKFSIGNFNSTPVFRGKKLTLTYNNGSYCPNGIDKKSTVLSFICDKEILAKASVSFVGVLHDCDYFFEVRTVHACPTAQKADNLALIWIFLFIFLAALMVYCSGGFLYKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P359</accession>
    <name>A0A1E3P359_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_78418</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038990.1">
        <property type="nucleotide sequence ID" value="XM_019184719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P359"/>
    <dbReference type="GeneID" id="30201965"/>
    <dbReference type="OrthoDB" id="10377204at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="174" mass="20042" checksum="8FF7AC4E4CAC4C7E" modified="2017-01-18" version="1">MSVFVPPGVKYRDILEDKHQLIDVQMLVNLFNSVAPKLQSILQNSNEEYIVAWGKLKNEISSQDSAIESESLILGIDMVYNNRYQGFLKNIDSFKDVSEKLAKRIDSHIQTNEKELLQLIMQSKSLVPSILDLFKDVDKLEEDIIHMFDELSDFLNEISSHYKALSNFRAKCTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P379</accession>
    <name>A0A1E3P379_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_30682</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019181752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P379"/>
    <dbReference type="STRING" id="683960.A0A1E3P379"/>
    <dbReference type="GeneID" id="30198998"/>
    <dbReference type="OrthoDB" id="5370059at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF155">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HERC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="2"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="164"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="349" mass="38741" checksum="4FD640D0135D6CDC" modified="2017-01-18" version="1">MFYVYSCGSNGRFQLGDGSDEDLNQLKRVFESKLRPVKVVCGGNHTLILLENGDLYAAGDDTFGQCGIDPETIGNNEQRVKVFSRVPRVDNSPWLDCSAGYEYSIFVNGNQQLFSAGFGLKGELGLGEKVGRSTKLTKIDTNFESNIIEVRSCLDHTVILLENHEVYGWGNGRSGKLGEPAQTKIWKPRLIETGSITHIEVGRDFTALGDTEGKIEILGKDKFNIASKLPQSWKLFKTMWSSLHFIDESNHIISAGNDSHGQLIPVEDVKFSHFDVGSEHGIITDGQKVKSWGWGEHGNCGINKIDSVTFNYINDLHQFPDTERIIMIKGGCATTWVVTEFLDSTKANE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P383</accession>
    <name>A0A1E3P383_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_63843</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038554.1">
        <property type="nucleotide sequence ID" value="XM_019183622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P383"/>
    <dbReference type="STRING" id="683960.A0A1E3P383"/>
    <dbReference type="GeneID" id="30200868"/>
    <dbReference type="OrthoDB" id="2109411at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050762">
        <property type="entry name" value="HD-ZIP_Homeobox_LZ_Class_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45714">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN HAT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45714:SF34">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN HAT9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="161"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="102"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="219" mass="25112" checksum="0F620C016D5496DB" modified="2017-01-18" version="1">MTTSDFSNYDLAVEKLDSVIQFSTKYSSKNERIIYDTVLIDDPFKKREVQKHVPERNRGQIIELIHGFEHSLERVSNSRYISKETILHNYKSIINEAIETAMDKEDRKSTEELDSDLSGQSSDPTQANDGDIGVANGSKKSLVPRNQKKEHKAQNSKTDENSKNKKRTSISKDAKAFLENVFSSKRTPNSKERKIIAEKCGLTPVQVRVWFTNKRMRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3D8</accession>
    <name>A0A1E3P3D8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_94805</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038604.1">
        <property type="nucleotide sequence ID" value="XM_019186460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3D8"/>
    <dbReference type="STRING" id="683960.A0A1E3P3D8"/>
    <dbReference type="GeneID" id="30203706"/>
    <dbReference type="OrthoDB" id="1919336at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF22">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION FACTOR A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00886">
        <property type="entry name" value="HIGHMOBLTY12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="4">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="2">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="42"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ59397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="68" mass="7894" checksum="4DD58B1DB60AD08E" modified="2017-01-18" version="1">MFFANDNRDIVRSENPGISFGGVGKLLGERWKALTPEEKVPYETKAEADKKRYEEEKANYQAQQQQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3F3</accession>
    <name>A0A1E3P3F3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="13">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_53195</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="13"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038941.1">
        <property type="nucleotide sequence ID" value="XM_019182707.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P3F3"/>
    <dbReference type="GeneID" id="30199953"/>
    <dbReference type="OrthoDB" id="270392at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02735">
        <property type="entry name" value="RNAP_I_Rpa1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01435">
        <property type="entry name" value="RNAP_I_RPA1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.100:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.120:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047107">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015699">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF11">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="15">
        <location>
            <begin position="328"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="262"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1315"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1371"/>
            <end position="1381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1382"/>
            <end position="1400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ59734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1635" mass="182136" checksum="DD58A1D3C7CE0F97" modified="2017-01-18" version="1">MDISKPVGSEITHVDFGVLSAEDIRKLSAKQITNPTVFDNLGHPISGGLYDLALGAFLRNLCATCGLDDKFCPGHQGHIELPIPVYNPLFFNQLYIFLRSACLYCHHFRLKSTEVHRYACKLKLIQYGLLSELEPLDNIHMSGASMEAEEGEEEDNAPDSNPQADRDLIERREDFVNSAIAKALSEGRTSENGVMTTTVLEERKHLIHEFYKKLLSRPKCGNCSMFSPTYRKDGFTKLFENPLKEKQVLNNRVKGHARQDMLKTNGKSNGDEMDVDTPAQPAKKSTGSKYVLSTEVRNIMRAVFQAESNILKYLFHSRPKATEKVNSDIFFLQAIVVPPTRFRLPSKLGDEIHENSQNELLSKVLNTSLLIRDLNDQISTVQKDKITAEERKITFNRLMNAFVTIQNDVNAFIDSTKNQNANAARTAAPGIKQALEKKEGLFRKHMMGKRVNYAARSVISPDPNIETNEIGVPPVFAVKLTYPEPVTSHNVNELRQAVINGPDKWPGAIQIQNEDGSLISLIGMTTDQRKALANQLLTPSGNTSHVLNKKVYRHIKNNDIVIMNRQPTLHKASMMGHKVRVLPGEKTLRLHYANTNAYNADFDGDEMNMHFPQNENARAEALNLANTDSQYLTPTSGSPLRGLIQDHISAGVWITNKDSFFTREQYQQYVYGCIRPEDGHSTRAKLVTVPPAIFKPIPLWTGKQIITTVLLNITPVNMPGINLLSKNKIKNEYWGEGSKENEVLFKNGELLCGILDKSQYGASQYGIVHSLHEVYGPSVAGKSLSVLGRLFTNYIQSTAFTCGMDDLRLTAEGNNWRNEILLTSVDVGRLAATEVTNLDKNTKSTDPKFLQRLEEVYRDDNKAGILDAVTQSKVNSITSQVVSKCVPEGTMKRFPYNSMQAMALSGAKGSNVNVSQIMCLLGQQALEGRRVPVMVSGKTLPSFKAYETDARAGGYIKGRFYSGIRPQEYYFHCMAGREGLIDTAVKTSRSGYLQRCLTKQLEGVHVSYDNSVRDGDGRLVQFLYGGDAVDVTKESHMNQFKFCIDNYDALLTKYNPGALIDHLDTESASRYSKKVRKSMKKNKDVPHFAQSTKYDPVLSVYNPSKYLGSVSETFQSKLDSFIDGNPDLFSKSGLSDKKFRALMQLKYMRSLINPGESVGIIASQSVGEPSTQMTLNTFHFAGHGAANVTLGIPRMREIIMTASAAIKTPQMTLPILDEVTDDQADAFCRSIAKVVFSELVDNVTVTETSGNSEDGRGKRSYDIDLEFFNSEQYHEEYDVSKEQLEQVITTKFLRTLESAITKELRKQKKIGSESLPEVGKAVPKSQTQITSDERISNKSNDNDDDDADDEKSRSKTKQSVSYDEPDDDEIETMRNAEKSSDEEMDSDSDSDSDSDSDEEEPTKSAATGELSKAARDRQSAVLGSHSLITRFNFDDVNGNWCKFKLELGAGTQKLLMVNIVEEICRKVVVKEVPHIGRCLHPQPEGGKRVLTTEGVNFGAMWDYDDFIDVNGITSNDVAAVLRTYGVEAARNTIVNEINNVFGRYAISVSSRHLDLIGDMMTREGSYLAFNRQGIDSSTSTFMKMSYETTCQFLTKAVLDNDREELESPSARIVLGKLSGVGTGSFDLLSRMPATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3N6</accession>
    <name>A0A1E3P3N6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_83650</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038719.1">
        <property type="nucleotide sequence ID" value="XM_019185576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3N6"/>
    <dbReference type="STRING" id="683960.A0A1E3P3N6"/>
    <dbReference type="GeneID" id="30202822"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000013">
        <property type="entry name" value="Related to multidrug resistance proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF78">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01036">
        <property type="entry name" value="TCRTETB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="311"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="378"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="402"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="448"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="519"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="50"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="551" mass="61490" checksum="7F026749A0DEED1F" modified="2017-01-18" version="1">MTSTSSIETIKREQLKIKDDEEVNTLEVHLSQVLADSHDNILPKKKLLIVFGALASALFLSFVDQNGITIALPDIAEELDAQETVSWAGTSSLISQTVFMVLFGRFSDIFSRKYVLIASMVILSLSDLACALAQTPYQLYIFRGFSGIGNGGITSLSMMIVSDIVTLEERGKYQGILGSCVGLGNAIGPFLASAFITHTHSWRKFYFMLFPLVISGCTFIVWLVPYTHHKQDMKKQFSQIDVLGFLSSSVTIIFLLIPISGGGSTYKWDSAFSIAFLCIGGVSFIVFLIVEKKFALLPMMPLHLFNTRVSLNFLFAQNFFFGICYFGMLYYIPFYLQSIRGFNTIDSSKYMLCMVFPQVFVSVISGQIISRHKHYWHVVWFGYTFWTVGMGLLLLWGTTTPISITIGALVVTGCGIGATFQPTLIAVQAQSYRKDRAIVISTRNVLRCFGGSVGLAVASTILSNFYNQKLTNYGASYFSSEEVKSLKSQVFSIPSFDKYSLIQVQFLKNIYMESLKNVFYLWIGCIAFCLLSNATVRDTGLEPLQENVNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3R2</accession>
    <name>A0A1E3P3R2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_84084</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039338.1">
        <property type="nucleotide sequence ID" value="XM_019185645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3R2"/>
    <dbReference type="GeneID" id="30202891"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="75" mass="8997" checksum="D368358342864177" modified="2017-01-18" version="1">MTTRNPSSLDSANCVTWLKTCRSPYIRYNVYVILRYKLEKREPSQLERLTKKSIKLPTDRSDKLRLNQLGFVWYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3W7</accession>
    <name>A0A1E3P3W7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Alpha-aminoadipate reductase</fullName>
            <ecNumber evidence="6">1.2.1.31</ecNumber>
            <ecNumber evidence="5">1.2.1.95</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">L-aminoadipate-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_53693</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(S)-2-amino-6-oxohexanoate + AMP + diphosphate + NADP(+) = L-2-aminoadipate + ATP + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46936"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="1.2.1.95"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(S)-2-amino-6-oxohexanoate + NAD(+) + H2O = L-2-aminoadipate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12308"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
            <dbReference type="EC" id="1.2.1.31"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(S)-2-amino-6-oxohexanoate + NADP(+) + H2O = L-2-aminoadipate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12304"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
            <dbReference type="EC" id="1.2.1.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pantetheine 4'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:47942"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.31" evidence="6"/>
    <dbReference type="EC" id="1.2.1.95" evidence="5"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039250.1">
        <property type="nucleotide sequence ID" value="XM_019182719.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P3W7"/>
    <dbReference type="GeneID" id="30199965"/>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00032"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004043">
        <property type="term" value="F:L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd17647">
        <property type="entry name" value="A_NRPS_alphaAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05235">
        <property type="entry name" value="SDR_e1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000046">
        <property type="entry name" value="L-2-aminoadipate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000787">
        <property type="entry name" value="L-2-aminoadipate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000035">
        <property type="entry name" value="L-2-aminoadipate reductase large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.30">
        <property type="entry name" value="Nonribosomal peptide synthetase, condensation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010071">
        <property type="entry name" value="AA_adenyl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014397">
        <property type="entry name" value="Lys2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010080">
        <property type="entry name" value="Thioester_reductase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01733">
        <property type="entry name" value="AA-adenyl-dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03443">
        <property type="entry name" value="alpha_am_amid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01746">
        <property type="entry name" value="Thioester-redct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44845">
        <property type="entry name" value="CARRIER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44845:SF1">
        <property type="entry name" value="L-2-AMINOADIPATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001617">
        <property type="entry name" value="Alpha-AR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="18">
        <location>
            <begin position="847"/>
            <end position="924"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ60043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1394" mass="154423" checksum="4075956080EFFC0E" modified="2017-01-18" version="1">MSSDLQHWAQILSSPTLSVLPHDFIRPSNNKVIEAKHSFSIPSKESWNSIISQVQGSNEFIVGLSVFAVLIYRLTGDDDIVISTDSPNGKEFVIRTTISPTISFVELVKQITTLYNDNVNRAVDYDELSALIQKENGLENKPQLFRLSFQYGNQHQQLSEYGSIRDLSVFVNDDTYDVYFNALLYKDARLSIFGEQVNQFFDAIAKNSSLEVTKVNLITKSQKEFLPDPTTNLDWSGYRGAIQDIFQENAGKFPERTCVVETPSFLTPEKQSRIFTYKQINEASNIVGNYLVKTGIKRGDVVMIYSSRGVDLMVSVLGVLKAGATFSVIDPAYPPARQNIYLQVAKPKGLIVIKKAGILDELVESYIENELDVISRVPQIELQDDGSVLGGVDISGKDVLADSPVTPTGVLVGPDSNPTLSFTSGSEGLPKGVLGRHFSLAYYFDWMSKTFKLNENDKFTMLSGIAHDPIQRDMFTPIFLGAQLLVPTADDIGTPGKLAEWMGKYGATVTHLTPAMGQLLSAQATAEIPSLHHAFFVGDILTKRDCLRLQTLAKNVFIVNMYGTTETQRAVSYFEVASREHDSTFLESQKDVMPAGKGMLNVQLLVVNRNDRSQTCGVGEVGEIYVRAAGLAEGYRGLPELNEEKFVKNWYVDQQKWVDLDNKSSKDEPWRQFWKGPRDRLYRTGDLGRYLPDGNTECCGRADDQVKIRGFRIELGEIDTNISQHPLVRENITLVRKDNNNEPTLISYIVPKVELEELENFKSSVEDLEKKDDQVVSGLVGYRLLIKDIKEFLKKRLASYAIPTIIVPFHKLPLNPNGKVDKPKLPFPTTAQLEAVAKHSSADQDDSQFSETEAKVRDVWLSVLPQRPSTISPEDSFFDLGGHSILATRMIFELRKQLVIDLPLGTIFKFPTIKAFAKEIERVQSGDAVALPSTSGASQDETTEVTKTADYYKDAQELSEKSLKSSYNSKTEIGKNDTINVFVTGATGFLGSFIVSDLLSRGSNIKVFAHVRAADEAKGFERVKKAGVTYGNWKDSFESSLEVVLGDLSKEQFGLDNEKWSYLTENIDVIIHNGALVHWVYPYDKLRDANVIGTVNVLNLAATGKAKYFSFVSSTSALDTQHFVELSDKLISEGKAGIPEADDLQGSAYGLGSGYGQSKWAAEYIIRRAGERGLKGAIIRPGYVTGETNTGSSNTDDFLLRMLKGCAELGKYPNINNTINMVPVDHVARVVVASALHPPKSDELAVVQVTGHPRITFIQFVGALKKFGYEIELEDYVSWKSSLENFVVEGTKESALYPLLHFVLDDLPQDTKAPELDDRNAVVSLKKDAEWTGVDASTGRGIDEAQLSVYVAYLIKVGFLPRPTSIGEPLPEITLSQEQFELVTSGAGSRGSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P427</accession>
    <name>A0A1E3P427_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_30780</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039161.1">
        <property type="nucleotide sequence ID" value="XM_019181760.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P427"/>
    <dbReference type="GeneID" id="30199006"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="531"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="579"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="606"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="634"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="743"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1178"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1207"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1258"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1288"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1440"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="140"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="834"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1492" mass="168562" checksum="2CA4E8D442A5A6D3" modified="2017-01-18" version="1">MSVIRNRSSSFERQSSDSSDRGSTREYGNGDEVTGNDYEEIARIVTDSHSQEEGGVLHKLETLSKQLSRRTAKDGPFEIDPNDFDLKRVLGAFFKSADEQGIHLRSTGVVLKDVTTIGVDAASSFAPTVGDLALFPITMFQKLKALRHRKTRKLIRNVNGVVEPGEMCLVLGRPGAGCSTLLRTIAGEHDQFIGVDGEVHYDHISQHEMMKKYKSDVIYNGELDIHYPHLTVDETFRFAIACKTPHTRINDASREQYVTAVRDLLATVFGLRHTYNTKVGNEFVRGVSGGERKRVSIAEALATKATVYCWDNATRGLDASTALEYAQAIRTSTNLSKSVAFITIYQAGENIYETFDKVTILYDGRQVYFGPADQAKDYFERMGFQCPPRQATAEFLTAITDPLGRYPKPGFEKKVPESADDFERYWHNSPEYKKMINQINDYEQKTDAAKTREIYDKSLSQEKPRAHYRYTINYFQQLRLLTKRGFQRIYGDKAYTITQTVAATIQALICGSLYYNTPSSTKGSFSRSGTLYFLILYFSLMGLAEISGQFTERPILLKQKSYSFYHPSAETIAATMTKFPFKFLSITILYLITYFLSNLTRDAGKFFLTFLFMVLSSETITALFQMVAALSQNVAGANAVAGVLMLAISLYSCYVIQLKSMHPWFKWISYINPIRYGFENMLADEFHGRKMDCGGTLVPSGDGYENVDSANQVCAFTGSETGQSWVSGDRYLEIQFNFKYSNLWRNFGIVIAFFVVFLAINAISTEFKRPVKGGGDHLYFKRGGKSSDKILMQHGSKNSDIETGVTNSQDNDLKSSNSSDELDVFEGLGSKSIFAWKNVDYVIPYKGSTRKLLDNVQGYVKPGTLTALMGESGAGKTTLLNTLAQRNDIGTVTGSILVDGKPLDTSFKRSTGYVQQQDLHIAEMTVRESLQFAARLRRPKSVPDSEKLEYVEKIIKILRMETYSEAIIGTLGSGLNVEQRKKLSIGVELVAKPSLLLFLDEPTSGLDSQSAWAIVHLLRRLAQAGQSILCTIHQPSATLFEAFDRLLLLEKGGKTVYFGDIGHNSRVLLDYFERNGARPCENNENPAEYILESIGAGATAQVEEDWYDKWCKSKEFEKTTKEIQALMQEGRDLPENDGAEKSELHKTYALPYWDQLYYVVKRNGTQFYRDPQYIGSKFFLLIIGGLFVGFTFWSLNDTIIGMQNGMFAVFLAIIISAPATNQIQERAVASRELFEVRESKSNTFHWSTLLIAQFVNEIPYHLVSGAMFFCCLYFPLRIDNEASRSAVWYLNYSIIYQFYYISLGLFIVYMSPDLASSNVITGLFLAFMISFCGVTQPMSLMPGFWHFMWKVSPLTYVIQTLMALVLHDKPVICAKDEYSYFEPPSGMTCQEFAGAYVEREHGYLLNGNGTSNCAYCKYSVGDDYLKSVSVSYSYVWRNFGFMWVYIIFNICAMCVLYYIFRVININPVDSIKKKIESQKLKREKKKLKSSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4G5</accession>
    <name>A0A1E3P4G5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein kinase RIO1</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Serine/threonine-protein kinase rio1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">WICANDRAFT_54034</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 22">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039450.1">
        <property type="nucleotide sequence ID" value="XM_019182729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4G5"/>
    <dbReference type="STRING" id="683960.A0A1E3P4G5"/>
    <dbReference type="GeneID" id="30199975"/>
    <dbReference type="OrthoDB" id="205248at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030874">
        <property type="term" value="C:nucleolar chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016479">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000234">
        <property type="term" value="P:positive regulation of rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007096">
        <property type="term" value="P:regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090234">
        <property type="term" value="P:regulation of kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05147">
        <property type="entry name" value="RIO1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000755">
        <property type="entry name" value="Homoserine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000148">
        <property type="entry name" value="Serine/threonine-protein kinase RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051272">
        <property type="entry name" value="RIO-type_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017407">
        <property type="entry name" value="Ser/Thr_kinase_Rio1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45723">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038147">
        <property type="entry name" value="Ser/Thr_PK_RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01245">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO kinase" evidence="24">
        <location>
            <begin position="77"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="386"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="389"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="425"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="23">
        <location>
            <begin position="464"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="20">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="20">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00068838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR038147-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR038147-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR038147-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="ODQ60243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="484" mass="56494" checksum="B4DEF3EEC8612D84" modified="2017-01-18" version="1">MSDLEERVGNLDIEDSGNISSDESDFSDFEDQKPSNNSATTTSTSKNTDDKAAILSKYSDRIKTDEFLFKNKQISKDKADRATFDNVLDPRTIGFIVKLLKKGTITKINGCISTGKEANVYHAVDETSGREYAVKIYKTSILVFKDRERYVDGEFRFRNSRSQHNPRKMIKIWAEKEFRNLKRLYSSGMNTPEPIDLKSHVLVMEFLSKNDDAGSPSPKLRDYQFEDFEEITKFYHQMIHDIRFMFQKCRLVHADLSEYNSIVHQGQLYIFDVSQSVEPEHPMSLDFLRMDIKNVNDFFMKKGIDIIPERLIFKFVIEDLKNLGIENDSTQDLMNYIENMPIKTTEEEIENDEVFRSIHLVRSLNHLEERDFDNFTAGKVDTLKALVDDRDEDDEEDEDQESDEESDDEDYDSADDEDDEEEDGDAKPKKKWVERPIELKGKKHEDKDAKKERKQKAKDDAKEKRKTKMKKHVKKKLVNKGKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4J4</accession>
    <name>A0A1E3P4J4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_68897</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039613.1">
        <property type="nucleotide sequence ID" value="XM_019184145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4J4"/>
    <dbReference type="GeneID" id="30201391"/>
    <dbReference type="OrthoDB" id="10472387at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="408" mass="46985" checksum="BAC1D6EDFF738309" modified="2017-01-18" version="1">MDSEKNEVAPGSNRVQQLIAGLKSTTLKNIKLLSSYQKDYEGFKTKYLELVEDFKNELSGVDENGGETAFKRNEYRKVLYLRRFDLIVKPITLRLKDPDIYNKTKEVLWKLIVQTCQVTKYKPGLVQYMYELNAFFNLAPPIFPDSNRKPQITLKFEDNQSIGNNGAIMIENFTNDIFEHDWHCKLAFEIYNSATFKLPKPSEFFSYNFLTRCQYSYHKEELITLIVANILKPIKTILSNQGFTLEIENNVNLRDLPDNSKLVDVSSFEFQLKADILLKYHKPISKSNMAAFTILVCEPSSLKEYIKDFKHDKGRYTLKSANFKNLVTQAFCLSVFVLNNFGIITDYNSFLLFKVDQDLKLECQKSSIMNKRAKNGGLRSSGFHCEVIHIKDGLCIAKLASFISDSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4R1</accession>
    <name>A0A1E3P4R1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Elongation factor 1-alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_62464</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039089.1">
        <property type="nucleotide sequence ID" value="XM_019183376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4R1"/>
    <dbReference type="STRING" id="683960.A0A1E3P4R1"/>
    <dbReference type="GeneID" id="30200622"/>
    <dbReference type="OrthoDB" id="3969558at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000211">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="22"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="475" mass="52042" checksum="45E44547CE92CCA2" modified="2017-01-18" version="1">MKRAANFKEFLCCLFLWKGKEKSHVNVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYHVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGIGEFEAGISKDGQTREHALLAYTLGVKQLIVAINKMDSVKWDESRFEEIVKETSNFIKKVGYNPKTVPFVPISGWNGDNMIEPSTNCPWYKGWKKETKAGEAKGKTLLEAIDAIDPPSRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMVVTFAPAGVTTEVKSVEMHHEQLTEGLPGDNVGFNVKNVSVKEIRRGNVCGDSKNDPPKAAESFNAQVIVLNHPGQISAGYSPVLDCHTAHIACKFDTLIEKIDRRTGKKLEEAPKFIKSGDAAIVKFVPSKPLSVEAFTDYPPLGRFAVRDMRQTVAVGVIKAVEKSDKAGKVTKAAVKATKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4U2</accession>
    <name>A0A1E3P4U2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_78541</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039119.1">
        <property type="nucleotide sequence ID" value="XM_019184746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4U2"/>
    <dbReference type="GeneID" id="30201992"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="123" mass="13004" checksum="3F7D34C5386952FC" modified="2017-01-18" version="1">MSLATPTVEAQVSLSGAETAKYIFSYTPEEPGTLVVTGEVALDSAAINIEQIAASPNLKEVYVNYGGSQLKGTWKYQDPSKKPARIVISGSQSGEVSNTSILFEGSYTFANGTLVEFSKEASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4Y3</accession>
    <name>A0A1E3P4Y3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Chromatin-remodeling ATPase INO80</fullName>
            <ecNumber evidence="15">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">WICANDRAFT_30168</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">ATPase component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the INO80 chromatin-remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The DBINO region is involved in binding to DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="15"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039169.1">
        <property type="nucleotide sequence ID" value="XM_019181695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4Y3"/>
    <dbReference type="STRING" id="683960.A0A1E3P4Y3"/>
    <dbReference type="GeneID" id="30198941"/>
    <dbReference type="OrthoDB" id="372624at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032006">
        <property type="term" value="P:regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18002">
        <property type="entry name" value="DEXQc_INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000022">
        <property type="entry name" value="Blast:Putative DNA helicase Ino80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001269">
        <property type="entry name" value="SNF2 family helicase/ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020838">
        <property type="entry name" value="DBINO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031047">
        <property type="entry name" value="DEXQc_INO80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF2">
        <property type="entry name" value="CHROMATIN-REMODELING ATPASE INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13892">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51413">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DBINO" evidence="20">
        <location>
            <begin position="201"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="444"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="993"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1177"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="273"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="375"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="44"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1200"/>
            <end position="1210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ59962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1232" mass="141609" checksum="F4743C61144A9E73" modified="2017-01-18" version="1">MFKQLSQLEFVKKESKKRKNASSNSSTTAATASSSSANAKTSKDQPVSATIASNGQSSIASKKKGKGKGRKPSISQPEPPAEDKRRTKKRKNSESNASIDENKPISLKLKFSKPPNGLVAIAPAPSDEPIEEKIGGKRKRVAKTNAEKQIEATSIANDDKLKKDVVKKQPPLTPVHSDKPKMNVKEMRAIKRQYDNTYFTIWKDLSRKDAPKVHRLIQQATQTKLINLRKTSILCAREAKKWQVRTNKSQKDLQAKARRGMREMFNFWKRNEREERELRKKAEKEALDKAKKEEEERESRRQARKLNFLITQTELYSHFIGRKIKTDEIEGTMAAGNNQSNPHHDVEVPTDAKRTEISALDFDSDNEEEIKKAAAANAQSALMDAKERAEQFNGNKNDLKEVNIDDDDLNFQNPTSLGDITIEQPKLLSCTLKEYQVKGLNWLANLYDQGINGILADEMGLGKTVQSISVLAYLAETHNIWGPFLVVSPASTLHNWQQEIAKFVPDFKVLPYWGNAKDRKILRKFWDRKQVTYTKDSPFHVLVTSYQLVVADIQYFQRIKWQYMILDEAQAIKSSQSSRWKSLLGLQCRNRLLLTGTPIQNSMQELWALLHFIMPSLFDNHEEFSEWFSKDIESHAQSNTQLNEQQLRRLHVILKPFMLRRIKKNVQSELGDKIEIDVYCNLTNRQQKLYKMLRSQISLMDLIEKATSGSDDSNQSLINLVMQFRKVCNHPDLFERDDVKSAFSFSKFAETGSFLRESNQLEVAYSTKNQLEYNLAKLIYQDLLLPNENNQVDVRDHVLNHLMNIWNSDYINENGKEDAAFSWLKFVDLTPSKASKVARKNIIESAIDMKKYVDEDDINQSIQDKKFLLVNDVSKLDCSELREVQQTVYDDMYLNVQDVAVHPAASAPPIELVCNDRSFVHNKDQELFNPKIRKALSPLSLDEQLHLMQNKVPLSQFPKAEMLPPVLNNKSGFSSIKMPSMNRFITDSAKLAKLDELLVDLKKNDHRVLIYFQMTKMMDLMEEYLTYRQYKYIRLDGSSKLDDRRDLVNDWQTRPEIFVFLLSTRAGGLGINLTAADTVVFYDSDWNPTIDSQAMDRAHRLGQTRQVTVYRLLVRGTIEERMRDRAKQKEHVQQVVMEGKATEQQPVGFQKPSREVAFWLLDDDEAADAVLKGKQLEEEKKAKKKQSRIQELNNKKSKKINLEDMYHEGEGNFDDGTNTNNTPSRVPTPIAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5C8</accession>
    <name>A0A1E3P5C8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PI31 proteasome regulator C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_62991</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome inhibitor PI31 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039641.1">
        <property type="nucleotide sequence ID" value="XM_019183472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5C8"/>
    <dbReference type="STRING" id="683960.A0A1E3P5C8"/>
    <dbReference type="GeneID" id="30200718"/>
    <dbReference type="OrthoDB" id="68090at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013886">
        <property type="entry name" value="PI31_Prot_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08577">
        <property type="entry name" value="PI31_Prot_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PI31 proteasome regulator C-terminal" evidence="3">
        <location>
            <begin position="225"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="160"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="209"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="273"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="334" mass="36457" checksum="925BE69E8947C2DD" modified="2017-01-18" version="1">MDANQTIHTQLKDKNSIVSISIEPKRGIPNVFTVVKLDDNDENFLFTSTISNITLSAIIKYDHFFASIQLPVQESTLERFVAENYSADRKGSLLDHLLKSFGDAGKVYETITKSAIGENAEWTDFYTSILIPAFQKFQKSEKVELSEQDTKTFIDIAKPTIGGRPMFSERTDPQTTGSSSSSMKSSSKPLPEELPKFDDEYELQRRKIHEDFIQRPSSSSPYAPIGDRDLHPFGQYPPMRPNLGPEAGSQSGGMIPDRNHPLFRQPSGGRGGLEGRGDHPPGSRYDDPLGRDDFDAVGKGLPGGMGLGRNSGAGPFGGSGTPFGRSSGFGGGFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5K5</accession>
    <name>A0A1E3P5K5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Protein PNS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_84073</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Probably involved in transport through the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cell membrane</location>
            <topology evidence="2 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039324.1">
        <property type="nucleotide sequence ID" value="XM_019185643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5K5"/>
    <dbReference type="STRING" id="683960.A0A1E3P5K5"/>
    <dbReference type="GeneID" id="30202889"/>
    <dbReference type="OrthoDB" id="44736at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385:SF4">
        <property type="entry name" value="PROTEIN PNS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04515">
        <property type="entry name" value="Choline_transpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="196"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="242"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="266"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="399"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="503" mass="55898" checksum="5C565B8E4AF447A9" modified="2017-01-18" version="1">MPIFNQQQAPNYYENNNNNQNQNQFVQPNEYGHTFDEAFQVEKPKWNDIPFTLFFIAVCCGFVAVAALTLRGYAQSYSQQGSGIYNSTSSFSLNTNTVILFVFVVVVALVISSATLMFARLHPKGFITTGIIVNIVMGLGTAIAYFVMHYYSAAVVFLVFTCISAFCYWGMRSRIPFSAEVLVTVIDVMKSYKSTLVVSAIGLLVSGIFSLLFSAVVVATYMKYDPKQGSEGCSVSGGGCSNAKLIGILFFVFFAGYYITEVIRNVIHVTISGVYGTWYYLSRSDEGQPRWPASGAFKRAMTYSFGSICFGSLIVTFIDLLKQGLNLLRQNAAAAGETCAQCGYLVLDILLGIIEWLARFFNQYAYSYIALYGDGYISAAKATWHLMRQKGIDALCNECLVGTALGFYAMFNAYVCALLAFLYVRLTKPEYNSEGTFYAPVVAFSFVIALQISNIVTQPLRSGTSTFFIALARDPEIYQQSYPNRFNQIASTYPNVFDKLRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5N3</accession>
    <name>A0A1E3P5N3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_78852</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039449.1">
        <property type="nucleotide sequence ID" value="XM_019184799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5N3"/>
    <dbReference type="STRING" id="683960.A0A1E3P5N3"/>
    <dbReference type="GeneID" id="30202045"/>
    <dbReference type="OrthoDB" id="3338076at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070916">
        <property type="term" value="C:inositol phosphoceramide synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070917">
        <property type="term" value="F:inositol phosphoceramide synthase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006673">
        <property type="term" value="P:inositol phosphoceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013862">
        <property type="entry name" value="Kei1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28077">
        <property type="entry name" value="INOSITOL PHOSPHORYLCERAMIDE SYNTHASE REGULATORY SUBUNIT KEI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28077:SF1">
        <property type="entry name" value="INOSITOL PHOSPHORYLCERAMIDE SYNTHASE REGULATORY SUBUNIT KEI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08552">
        <property type="entry name" value="Kei1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="207" mass="24054" checksum="449DDAFAAF030D8B" modified="2017-01-18" version="1">MSLINKLPLPNQFLGVLPLYIGVELILGIAILNKLSGLYGILSIFTGHPLDLVQWLLYITSILIVPCYAIGFRHIQKPKILEFAPILVLYTIDSLTSLGFIVWFLAEWLINEDVKIEKIEGEDYSKSASQGYEYGWIFLTTIGINVSRVYFNLILLSFYKKLIRFNKLQGIETLQNGEELDLKNQYFWVRWNYKFQKASYNFLKNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5X0</accession>
    <name>A0A1E3P5X0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">uS12 prolyl 3,4-dihydroxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_27794</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[ribosomal protein uS12]-(3S)-3-hydroxy-L-proline + 2-oxoglutarate + O2 = [ribosomal protein uS12]-(3S)-3,4-dihydroxy-L-proline + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54160"/>
            <dbReference type="Rhea" id="RHEA-COMP:13817"/>
            <dbReference type="Rhea" id="RHEA-COMP:13818"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:85428"/>
            <dbReference type="ChEBI" id="CHEBI:138052"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[ribosomal protein uS12]-L-proline + 2-oxoglutarate + O2 = [ribosomal protein uS12]-(3S)-3-hydroxy-L-proline + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54156"/>
            <dbReference type="Rhea" id="RHEA-COMP:13816"/>
            <dbReference type="Rhea" id="RHEA-COMP:13818"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:85428"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TPA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040032.1">
        <property type="nucleotide sequence ID" value="XM_019181410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5X0"/>
    <dbReference type="STRING" id="683960.A0A1E3P5X0"/>
    <dbReference type="GeneID" id="30198656"/>
    <dbReference type="OrthoDB" id="430522at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019826">
        <property type="term" value="F:oxygen sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031543">
        <property type="term" value="F:peptidyl-proline dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140311">
        <property type="term" value="F:protein sequestering activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000639">
        <property type="term" value="P:negative regulation of SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006449">
        <property type="term" value="P:regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006415">
        <property type="term" value="P:translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000014">
        <property type="entry name" value="Prolyl 3,4-dihydroxylase TPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.20">
        <property type="entry name" value="Oxoglutarate/iron-dependent oxygenase, C-terminal degradation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019601">
        <property type="entry name" value="Oxoglutarate/Fe-dep_Oase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043044">
        <property type="entry name" value="TPA1/Ofd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039558">
        <property type="entry name" value="TPA1/OFD1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051842">
        <property type="entry name" value="uS12_prolyl_hydroxylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12117">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12117:SF0">
        <property type="entry name" value="PROLYL 3-HYDROXYLASE OGFOD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13661">
        <property type="entry name" value="2OG-FeII_Oxy_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10637">
        <property type="entry name" value="Ofd1_CTDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="14">
        <location>
            <begin position="144"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00081607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ60825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="597" mass="68971" checksum="DCB77FEF0AA56C9F" modified="2017-01-18" version="1">MCVPKRSTEEAPAQDSKKQSTQFQDRTDVKQYFGPGIFDEKFRKDLTQTISDSEPYRWGTIKNLMDDTLLRNVRKEIENEIHFTKKETDIYKVFQSGDLANLSGLDWDDLSRLPSLFKLREALYSQEFRDFISEVTQSGKLSGTKTDMSINTYTKGCHLLTHDDVIGSRRVSFILYLPEPDKIWKEHYGGALRLFPSIVPNVPKIDPSAKLVPQFNQIAFFHVQPGLSFHDVEEVRVDKQRLSIQGWYHIPQPGEDGFIEGEQEKTEARSTLQQLESKELEVFDFPKEVRIPFSSHEVKYYENFEGLDKIDLEYLSKFLNPALLRLEEIKRLQKVFIDESLIEIKDLLNEELADELKKKIRNTELDEIMPKHQDEVEFPWKISLPPHKWRYLYMDGKQEEPLTKEGVTNSNLGPQEGPNFKLLAKTTKHESSLKLIELASFLKSTSFKKWLSLITDLVPISDQILIRRFRPGHDFKLATSINNEDSELDALLEATLSLTPSKGWESGELGGYELGMVTEDPEDGDEDDPAIYKSSKSEDDSVVVNNQASWNHFSLMLRDPSVLKFIKYVSYNAKGSRWDISCQWNVKTKGEDEEDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P692</accession>
    <name>A0A1E3P692_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_62928</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039574.1">
        <property type="nucleotide sequence ID" value="XM_019183461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P692"/>
    <dbReference type="STRING" id="683960.A0A1E3P692"/>
    <dbReference type="GeneID" id="30200707"/>
    <dbReference type="OrthoDB" id="244495at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF27">
        <property type="entry name" value="PHO85 CYCLIN CLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="43528" checksum="795B8667D1F9D769" modified="2017-01-18" version="1">MSAIYSYQPYQPVDQHRHHSGGYYQPTVASNGYYQGQYIPQNVAAPMYHHPQPQAPVMQHQIYAQPPAMAAPAVNPYMQGNAYGYNNTNAYYQVPQQQQYAQPQQQQQQQEQQGEHAVTGGVSSVLDYDLSAMSKFATYLAFRLFARSDTENTKFVSSLKSVLSATRLPLSSLILANYYILQKYELDPASFNNSADELIYQNVVISLVLANKANDDNTFINKSWAAATGLSVKLINVLEANWLNLLDWKLHDVQMEKYDELMLQFNRYSKGNTTPQRSSRYVRDPISPILPSSSQSPRDNSPSRFNNYSSTNWYSEYTATTPTPYSYEGFAPSSYGHQKSYSFNEPEYCSRTGSYQKMMYCTCRYCSTPPRSVDWSYNVAAAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6E9</accession>
    <name>A0A1E3P6E9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RWD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_90253</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IMPACT family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040203.1">
        <property type="nucleotide sequence ID" value="XM_019186222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6E9"/>
    <dbReference type="STRING" id="683960.A0A1E3P6E9"/>
    <dbReference type="GeneID" id="30203468"/>
    <dbReference type="OrthoDB" id="69641at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140469">
        <property type="term" value="P:GCN2-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23822">
        <property type="entry name" value="RWD_ScYIH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.30">
        <property type="entry name" value="Impact, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023582">
        <property type="entry name" value="Impact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001498">
        <property type="entry name" value="Impact_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036956">
        <property type="entry name" value="Impact_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020569">
        <property type="entry name" value="UPF0029_Impact_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301">
        <property type="entry name" value="IMPACT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301:SF25">
        <property type="entry name" value="PROTEIN IMPACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01205">
        <property type="entry name" value="Impact_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00910">
        <property type="entry name" value="UPF0029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="RWD" evidence="7">
        <location>
            <begin position="8"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="260" mass="29961" checksum="E62ED3E74B919031" modified="2017-01-18" version="1">MVSEDLRDELDAIEAIYPECLTRMNENLIDLKIPQHEEYVVRMSFPEKYPESEIPHILQVRSNGHDDQYLETLFTEVLESIYTEGNVCIFDFCTELEGILYTEEEPEEPIVQEEPEDLKVDLLEGWTASEPVYDRGSTFMAFVRKVDSEDELFEKLALLKSERKVSKANHNMTAWRIQNSDNTRVQDCDDDGETAAGGRLLHLLTIMDVWNVIVVVSRWFGGTHIGPDRFKHINSTAREAIVKAGFVDENDSKKSKKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6K2</accession>
    <name>A0A1E3P6K2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_79072</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039684.1">
        <property type="nucleotide sequence ID" value="XM_019184836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6K2"/>
    <dbReference type="STRING" id="683960.A0A1E3P6K2"/>
    <dbReference type="GeneID" id="30202082"/>
    <dbReference type="OrthoDB" id="3979207at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044852">
        <property type="entry name" value="WBP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31606">
        <property type="entry name" value="WW DOMAIN BINDING PROTEIN 2, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31606:SF1">
        <property type="entry name" value="WW DOMAIN BINDING PROTEIN 2, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="157" mass="17961" checksum="25CF5F466340EE2B" modified="2017-01-18" version="1">MSLNWVMRTPGTEYPPFVLLENEQVKFSPPEIVDIKLKPLELKNPDTIGLKGKIYLTTHRIVVLSSGKTGRENNDSFALLYKDIQSHKLEMPWFGNNRYLILFKISNPDGGLNYLYPWSLVVDFTSGGAIRFAEEFSTIKTRFDNGQLVDELPAYTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6K5</accession>
    <name>A0A1E3P6K5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_61627</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040268.1">
        <property type="nucleotide sequence ID" value="XM_019183219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6K5"/>
    <dbReference type="STRING" id="683960.A0A1E3P6K5"/>
    <dbReference type="GeneID" id="30200465"/>
    <dbReference type="OrthoDB" id="2534600at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052783">
        <property type="entry name" value="Metabolic/Drug-Res_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47655">
        <property type="entry name" value="QUINIC ACID UTILIZATION ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47655:SF2">
        <property type="entry name" value="QUINIC ACID UTILIZATION ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="4">
        <location>
            <begin position="89"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="479"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="599" mass="67661" checksum="9385C9CE77A7CC60" modified="2017-01-18" version="1" fragment="single">FAIVRYTHKSNVKDPNIDGNFIGLIWAILALGQFKFNGFINEESKILSLNCARNSVIALETTTTSTIETIQSMLLLGLFYYEIGEWDSAWVLTSSASRMAVDTRLMINNNQKLMEHEIKLRERTWSGVYLLNLLLSLRLGRSPVVRKADYPIPKISEDDWEEWDGWTEFQIENYENLTLANKKPLKLESARCLSVHNKLLELASLLNLIVTSYIEYNENLDQTGFYKNDHHRLLNTNPEVFTFGTYTINPNETIAVSAIEDELLEWFKSLPPHCQINDDTSDEEENGKIPHFVALIHLFYHLSWGLLALKVLDIKHIDASDTIKVLNHLNVNVHFAKSLKSIIEILQRFNVKSLVLSIPYIDYFLSVPFKLNDVLDKDYASEIMDILQQASTISPSCLVTYNILKIMQQQDQNKRSLMATYKEQQQSHHQPIPAETLAQGTSDSELLNSFKQASGMNSLKHTPTPDLQQRSYFARPQSTSFKPISSFNSPNPSGTTATSTDPSTMINDLDSFLIDIGPSNKGENKMKQFIKNLGYVGNSKSSIYDHVTTPQIASSSSTGAMGTMGTALNENLAFTPGEIESIGLHEYLEHMAASNSKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6N5</accession>
    <name>A0A1E3P6N5_WICAA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_77732</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040293.1">
        <property type="nucleotide sequence ID" value="XM_019184601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6N5"/>
    <dbReference type="GeneID" id="30201847"/>
    <dbReference type="OrthoDB" id="3982796at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="261" mass="30860" checksum="387D34530FA163E9" modified="2017-01-18" version="1">MSPRQREFILKVNKELFLEFGEVPEKRKEFLKAKRLLDKKHRHGSEFKDHQELFEVFKSITEDQSIKRNTQIIAKINLGEMSLILTQDLFQYVQIGDDLYDLNSRFYEFSRRIYDYCLVQSFLDYTPELGLFMKILDFKMQRLFATEKYKSPSQRKDTFLVHMAMDKAAGINAPLVKATEKFMKLYPKEVTRFISSSPNKDRNPQDFTDIPDSRCVRRRMKGVPFTNNGARKYLLKDARELLESEDEPPARQESVVRADSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6U8</accession>
    <name>A0A1E3P6U8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fork-head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_27692</name>
        <name evidence="8" type="ORF">WICANDRAFT_29242</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
            <strain evidence="9">NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040208.1">
        <property type="nucleotide sequence ID" value="XM_019181588.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040300.1">
        <property type="nucleotide sequence ID" value="XM_019181400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6U8"/>
    <dbReference type="STRING" id="683960.A0A1E3P6U8"/>
    <dbReference type="GeneID" id="30198646"/>
    <dbReference type="GeneID" id="30198834"/>
    <dbReference type="OrthoDB" id="3980872at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00059">
        <property type="entry name" value="FH_FOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000260">
        <property type="entry name" value="Forkhead transcription factor (Sep1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881">
        <property type="entry name" value="CHECKPOINT SUPPRESSOR 1-LIKE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881:SF1">
        <property type="entry name" value="FORK HEAD PROTEIN HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="7">
        <location>
            <begin position="11"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="11"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="86" mass="10009" checksum="CFE090388777167E" modified="2017-01-18" version="1" fragment="single">MPKIVHDSDAKPPYSYATLIGMAILRGEDRKLTLSQIYSWISSTFKYYRRNDVGWQNSIRHNLSLNKAFVKTEKSSDGKGHYWEIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6V8</accession>
    <name>A0A1E3P6V8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_83383</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040378.1">
        <property type="nucleotide sequence ID" value="XM_019185533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6V8"/>
    <dbReference type="GeneID" id="30202779"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="91" mass="10749" checksum="31F4529327A3C084" modified="2017-01-18" version="1">MALDLQVVNIKLLAARLRCWTCPTSTLFDWSQLVINNYKITLVKTCECRRMANKVECNDWVFLQVEANLCYFNLYRSRIFVNMKSIIHISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6W2</accession>
    <name>A0A1E3P6W2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Gfo/Idh/MocA-like oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_83246</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040171.1">
        <property type="nucleotide sequence ID" value="XM_019185509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6W2"/>
    <dbReference type="STRING" id="683960.A0A1E3P6W2"/>
    <dbReference type="GeneID" id="30202755"/>
    <dbReference type="OrthoDB" id="3978523at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051450">
        <property type="entry name" value="Gfo/Idh/MocA_Oxidoreductases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377:SF1">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase C-terminal" evidence="2">
        <location>
            <begin position="139"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="42338" checksum="33CCBA8A9C9ACEC9" modified="2017-01-18" version="1">MSQLKLIVCGLGLIGPRHAEQIAQNRETELIAFIDPSPKAQLIADQFNVPLYSSLTELLNAGLRPDAAYVCTPYALHVSVALELANEGIHILVEKPISTKVEDAIALKDCVESKGVKLLVGHHRRFNPYILATKKHLSKLGAIIAVDGVWTLKKNDTYFKQVYWRNVKSLGGGPILINLVHDLDLLQYLMGPIKRVYAEKLSQHRDVVESDDPVDEGAVLTLSFKSGAKGTFIVSDNVISPFNFEMGTGENPLIPKVEDPTDGVFYRIFGTKGTLTVPDFHYYHQNDLKPGLEKGWWEPLHKTVLEKDITGVPFALQLDHFVKYVRGEEDAKCTITDGISALLVIEAIMESLESGAPRNVKSVSSFTSSLKNKDDDPFKSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6W8</accession>
    <name>A0A1E3P6W8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Actin cortical patch SUR7/pH-response regulator PalI</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_104291</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040388.1">
        <property type="nucleotide sequence ID" value="XM_019180410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6W8"/>
    <dbReference type="GeneID" id="30197656"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052413">
        <property type="entry name" value="SUR7_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019">
        <property type="entry name" value="CELL MEMBRANE PROTEIN YLR413W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019:SF2">
        <property type="entry name" value="CELL MEMBRANE PROTEIN YLR413W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="39936" checksum="F81830A5B9346166" modified="2017-01-18" version="1">MRRNTSKPSTLAHNHMGSHFTYKFFNHIVPALIALSSVILLGMALSGQTRGMRQQADIYLLKFDFSDIREKNVFISTNPSFYSLYYDLRYRHFAIGPWGYCKSKYSAFSSWNTYCYSRKAPYQYDLYELLEDDAILYSNRKYIDYIALPENTQIPQNMVDTIFVTLWISTVTAGVSLLFYFLSFITTTRDKPGCTNILLTLISAIASFICGGVARGSSSKIIKTLEKTHKIFGITATLGNKKFYNHLWAAGALQIFSVLFVLFVDYKLAKAIENEPDEESTPQTSPPRSDPTPTTQRPRETIPQRPIRSPAPANVSISIRVNRPATVEIDQPPRYEEIDDHHPPKPPPFTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P759</accession>
    <name>A0A1E3P759_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_61750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040395.1">
        <property type="nucleotide sequence ID" value="XM_019183239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P759"/>
    <dbReference type="GeneID" id="30200485"/>
    <dbReference type="OrthoDB" id="786951at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025239">
        <property type="entry name" value="DUF4187"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039249">
        <property type="entry name" value="GPATCH11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21032">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21032:SF0">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13821">
        <property type="entry name" value="DUF4187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01173">
        <property type="entry name" value="DUF4187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="137"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="353" mass="40730" checksum="52F68F60AD99FEEE" modified="2017-01-18" version="1">MSLRKRAALGFDDGSSDEEVEKPQPLCKSKLPKLSFKTQEIKENDIDDVRPTLGKEEDPEAILGGDDDYMRMAITEDEKTGPQRLQPKDETKTKPGYNFEEIQDKKESKPASLFGQLESSRADDSTLQPMPKSQLKNSSKGLSIMEKMGFKIGDTLGKDSKNKKAILEPILVLQREDRTGIKEGQIKIGKSNKTKAFEGITNEDAKEFRERLTDEQNTQRQEKILHKMQKYCLEFSGDGDAPDIEKVDPLGINVLWRGYVKFVQKLLKDKREQRRIKNPEEIQEIQQDGENSEEEDNDEELELFEELTIEEKVRKLHLFLRTEFDYCYYCGAKYEDQGDLYEHCPGFTEEDHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7F3</accession>
    <name>A0A1E3P7F3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_90751</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISP family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040552.1">
        <property type="nucleotide sequence ID" value="XM_019186257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7F3"/>
    <dbReference type="STRING" id="683960.A0A1E3P7F3"/>
    <dbReference type="GeneID" id="30203503"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015908">
        <property type="term" value="P:fatty acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015918">
        <property type="term" value="P:sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05384">
        <property type="entry name" value="CAP_PRY1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000012">
        <property type="entry name" value="Secreted protein PRY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012045890" description="SCP domain-containing protein" evidence="6">
        <location>
            <begin position="16"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="7">
        <location>
            <begin position="76"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="212" mass="23857" checksum="7B5616E8F59E6FE4" modified="2017-01-18" version="1">MLWIRYSLLVSPLLAKTVTLHQTNTHTIQEVQTEVHTTLITRWSTIQHTDEPTTTTTITRTTTTTIDIDPSSSPSSFPSQVVDLHNYERSKHSAQPLTWSSELTNFAESYATNYNCNGTLIHSHSKYGENLALGYNTSAAIKAWYDEVKLYDFNNPGFRESTGHFTQLVWNSTKYIGCAVKDCGSYFGQYLVCEYDPPGNFQGAFDDNVFAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7F8</accession>
    <name>A0A1E3P7F8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_104389</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040505.1">
        <property type="nucleotide sequence ID" value="XM_019180414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7F8"/>
    <dbReference type="STRING" id="683960.A0A1E3P7F8"/>
    <dbReference type="GeneID" id="30197660"/>
    <dbReference type="OrthoDB" id="4069891at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="386"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="533" mass="60241" checksum="BAF12918666CAD45" modified="2017-01-18" version="1">MVRISPSKNKNLSPNSVGKLQFASIDNSEVEHQLKRIIDSQASLRNIIIGVDTSVKQTQLDLENLVERSSNNNSHLKDLVQNVKSSVSTPDQNILSKEVIQEVMSEALVPLKHNTDSTDALKKDIIKKIDLLDSKVNGEETPASKSLKELKDHIQSLSSLVHHIKSQNSDYTSLITTLGEKLSNNENNFTESIKALTEESKREILNSISTIGDSIQGFAPKDFFDQSLKKSNEILKFLEGQNSTKSETLEAQFHGLQSSHKALTKDLKETLNNSQDKHTNHISELITLINSSEEESSRSKLSEESITDKILNSNAELSKELQQEFVKLHDLASNNHEIILESLKDDISKKLLALESKTETLEKGINDSEESKKHINKEIENRDLQIQLKDKEIAELKAQLDERTKKEEINESVIELSQTKAKLESKYDQLVQSYRERYSEFKELSGDYDALQLKLSNLSLEKMKNVLGAATLMKMSNENINSSDKVESPKKNVLGSRVLSTNSYLNTNQLQLTPKKKYEFETTSEEFEEKENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7N0</accession>
    <name>A0A1E3P7N0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">serine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Seryl-tRNA synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Seryl-tRNA(Ser) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_61857</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040507.1">
        <property type="nucleotide sequence ID" value="XM_019183260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7N0"/>
    <dbReference type="STRING" id="683960.A0A1E3P7N0"/>
    <dbReference type="GeneID" id="30200506"/>
    <dbReference type="OrthoDB" id="10264585at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070158">
        <property type="term" value="P:mitochondrial seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="206"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="301"/>
            <end position="303"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="317"/>
            <end position="320"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="388"/>
            <end position="391"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="8">
        <location>
            <position position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00034892"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ61300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="467" mass="52993" checksum="4028D972995ACB03" modified="2017-01-18" version="1">MLRRGNILSLRSLNVRHYSAKVNIKIENSTTLKKPVYNFKGISENLDHYLLSAKRRELPQDVLKEIQNTGEYYAKVKDLTASLTKIRAEKNISQVTLKELRQKKEIGAKYEEQLKIQGMLKKLEKEINENIKAEELKLYGVMESFPNALHESVKDEQELVKYLNPKDQYEPIPELEHSKIAQDFGIVDLKAGSTVSGSSWYYLIGDGALLEQALVQYALKLARKHGYKMVTPPSIVKVEVSDACGFKPRDQNGEVQTYELKQDGLVLTGTAEIPLAGLAINKTFNEQDLPERVVGVSRSYRAEAGARGRDTKGLYRVHEFTKVELFVWAAENQSEGELEKLREFQEELITSLGLSARVLNMPANDLGAPAIKKYDIEAWMPGRGNWGELTSTSNCTDFQSRRTHTKYKNKELQKSFFVHTLNGTALAVPRVIVALIENFYDPSSNRIAIPKVLQSYMDDKEFIEKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7N8</accession>
    <name>A0A1E3P7N8_WICAA</name>
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        <recommendedName>
            <fullName evidence="4">Ribosome quality control complex subunit 1</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">WICANDRAFT_83571</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ61435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019185564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7N8"/>
    <dbReference type="STRING" id="683960.A0A1E3P7N8"/>
    <dbReference type="GeneID" id="30202810"/>
    <dbReference type="OrthoDB" id="205993at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006994">
        <property type="entry name" value="TCF25/Rqc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684">
        <property type="entry name" value="NULP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684:SF0">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT TCF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04910">
        <property type="entry name" value="Tcf25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="636" mass="73113" checksum="40C55D3523B07CED" modified="2017-01-18" version="1">MSSRALRRLEKQREQTRLAEENNDVEEVEEESVEPVKKAQNIFALIGGEEDDDEDDEDQEKVESETEDVKASVHLPTKSQKKNKKKKNKGKKKAEPKEVDEDSDDELDKIIKELNRKENRIVDTTEIDAEEEEAIDDEVTHEILDSSFSHFTPLKQYKSASLLKIDPRDLDPDNEFKKLFGNLSAEALNDADSTTSTSVPPEILQQIKRLSKVVRGWGGRDRRSIPGTTRKLILTKIRDDWIPTPKKDINMEELSIKEVIDDKAAESEDWVDVIKDDVVKMAKYGIKHYKFERGAESKIANSQFYASVVVTPNHENLMQLLPRFPYHVETILQVALILIRQGDKSNSNGLVERALFTFDRGFKQNFDLSNGLTRLPFNYYLNRQFYLAVFRYIEILSKKGTLFTAFTYAKLLLALSPADDPYGVRYFIDFYAIVSAEYKYLTEIVDSPLVTTYSKWYTPGIAYSTVLAYLYLGNERKAKEYLKKAFIKHPFTGYKLLEAIGLASDIPVNDYSIFEVGQETELSTEAYLLRAPIVWKEANHRKFLHDTLLGFINNKIKFQPTQDGVIPMNFLRFAILSGEGKVMAKIPPEIWEQNEIYEFDVLPPKLGYNDEEIMEMVRNLSLQDAIDQQVPGAFPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7X5</accession>
    <name>A0A1E3P7X5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit E N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_61537</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040184.1">
        <property type="nucleotide sequence ID" value="XM_019183196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7X5"/>
    <dbReference type="STRING" id="683960.A0A1E3P7X5"/>
    <dbReference type="GeneID" id="30200442"/>
    <dbReference type="OrthoDB" id="417252at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016650">
        <property type="entry name" value="eIF3e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019010">
        <property type="entry name" value="eIF3e_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10317">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09440">
        <property type="entry name" value="eIF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01186">
        <property type="entry name" value="eIF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Eukaryotic translation initiation factor 3 subunit E N-terminal" evidence="4">
        <location>
            <begin position="16"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="161" mass="18992" checksum="2A37DFD719FED8B7" modified="2017-01-18" version="1" fragment="single">MSELGAAELEIARKYDLTKKVIPFLDRHLIYPILESLRSEDLYDDKAITQLTFDLFKETNMISFVKEQWKTLNPNAQVPKELEEKEAKVDEIFNKLNNETKETLDILNLPEVQDHLKQDKQFNREYLEKNHGITESKINALYEFGQFQYNRGDYVMASDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8P8</accession>
    <name>A0A1E3P8P8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminopeptidase P N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_24762</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040992.1">
        <property type="nucleotide sequence ID" value="XM_019181054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8P8"/>
    <dbReference type="STRING" id="683960.A0A1E3P8P8"/>
    <dbReference type="GeneID" id="30198300"/>
    <dbReference type="OrthoDB" id="4215474at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01087">
        <property type="entry name" value="Prolidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007865">
        <property type="entry name" value="Aminopep_P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052433">
        <property type="entry name" value="X-Pro_dipept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226:SF4">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05195">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01011">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminopeptidase P N-terminal" evidence="7">
        <location>
            <begin position="51"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="502" mass="56258" checksum="B9AB7A9A615450C2" modified="2017-01-18" version="1">MLRFKIPSLRSSIKCFCRGYSQRPTPPFTAGQPLFETRPFMVKPGELTPGITALEYFQRRIDLASKLPDSSLAIVVGSQVQFASGSVFYDFQQNNDLFYLSGWNEPDSVIVIEKPNNKLEDVIFHMVVPPKEPLAEQWDGFRTGVEGAVEVFNADESEQIYRLAPYLDKLVQRNKNVFIDLPEGTKKQTTSSTFTNFFNIGGNKKLTSVQELLKTSGSGKSIKPLSPFITEMRTIKSDAELRIMRKAGQISGRAYNQAYAKRFKTERTLKSYLEYRFIAGGCDKPAYIPVVAAGSNALCIHYTRNDDLFYEDEMVLVDAAGSLGGYCSDISRTWPSSGKFSPAQKELYQAVLNVEKECITNCTENHNFSLQDIHDLSVGLMKRELRNCGFGELTYSDITKLYPHYIGHNLGLDVHDVPRHSRTAKLKAGQVITIEPGVYVPDDHAFPASFRNIGIRIEDDIAVGKDTYTNLTVEAAKEIVDIERIAEVGVTTPLDDEVVEVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8V5</accession>
    <name>A0A1E3P8V5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GTP cyclohydrolase II domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_66170</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase II family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041038.1">
        <property type="nucleotide sequence ID" value="XM_019184032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8V5"/>
    <dbReference type="STRING" id="683960.A0A1E3P8V5"/>
    <dbReference type="GeneID" id="30201278"/>
    <dbReference type="OrthoDB" id="57939at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003935">
        <property type="term" value="F:GTP cyclohydrolase II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00641">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10990">
        <property type="entry name" value="GTP cyclohydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022163">
        <property type="entry name" value="GTP_CH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032677">
        <property type="entry name" value="GTP_cyclohydro_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000926">
        <property type="entry name" value="RibA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036144">
        <property type="entry name" value="RibA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005536">
        <property type="entry name" value="PRK07198.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47259">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47259:SF2">
        <property type="entry name" value="URACIL-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12471">
        <property type="entry name" value="GTP_CH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00925">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142695">
        <property type="entry name" value="RibA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GTP cyclohydrolase N-terminal" evidence="6">
        <location>
            <begin position="37"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="GTP cyclohydrolase II" evidence="5">
        <location>
            <begin position="265"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="450" mass="49876" checksum="541D5C37D47AF37D" modified="2017-01-18" version="1">MAPAAILQQQALTDHIHNGSACTHELKLKNEVPLDKDVVLTTFTNGAGINPLPLNWGASDPQVRGPIVASRIKQGLSKHNAIGAHSGSYCIYHALAVGSKQLNPNYDADYTNAQPAFKIPTQAQWSDGEKIVSMDPFGHLTPWLFNEVGELEDVEIRPTISVTKAHMQLFEMKDAVEKGRLQVDGEVVINSNGDLNVSKVAVEPVWYLPGVAKRFGITENELRKALFEDTNGMYPELITRPDIKVFLPPIGGLTVYIFGNPEDISNPDKKLALRVHDECNGSDVFGSDICTCRPYLIFGIEEAVKEAQNGGSGVVVYFRKEGRALGEVTKYLVYNARKRGGDTAAEYFHRTECIAGVRDMRFQQLMPDVLHWLGITKIDRMLSMSNMKHDAIVDQGIPIIERIPIPDELIPPDSRVEIDAKINSGYFTNGKILNTEELKDVKGRGWNDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9B1</accession>
    <name>A0A1E3P9B1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_62025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040674.1">
        <property type="nucleotide sequence ID" value="XM_019183297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9B1"/>
    <dbReference type="GeneID" id="30200543"/>
    <dbReference type="OrthoDB" id="3980557at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035293">
        <property type="entry name" value="Vac17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17321">
        <property type="entry name" value="Vac17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="644" mass="72834" checksum="1A4CDC26D0ED2653" modified="2017-01-18" version="1">MIQQEQCTGATIPVLDSITDRLLKKSNNAIGKIEENIKLTSSLRNSNSLISQIDTKIDGLTSNLQQYQFYLSQLNSLLIRSEFFEAQLNMLEAHNKLNSLRSSNGMPSLNDWKDEDLNWRTTEATLRNLYLEYQYLTKQMEQLSDPQSFNSFIISQHDQQHQQEPEPQQLHQPTLQDKMDNQYANLKPIRIVSKRERRRSMDYSKKKPIAQIPNLLKHKNQQELPTPDTTFDKSFNYSIDNETAIYDTVLPSNLTTNNQPTSRRNSHNKHTRCASLPESIVSSMLDTVRFTLSDDGLAHETKKDRKFNLRHFISHETGLKTKCIHDEEDEDVKDSEFLHSPVSIKLESYLKEHVNREEPSKVSEEDILIEPHEEEVTVFEHKLNRSNSCDSIFSMMKPEPLIPRDNTQQTMSWMKQFAKPQSSYAKESNVTTISINNLSSSISSPSRQALSNLVTQNTTNASIQKPQNKFPFLFAAKRSSPLAAPATTTTTTAVSEQSATVSSIPASVKRQLSVDDSSSYHSFESSYSDSQQHKQWSLLLNKFKPKNLMPSHSLVTSEVGKPIAQAQPIPISSTGSNPIHMHNHSKTLGAEKIKGSFSTLTIGPNRSKIVQHGESSTLGQSLVVSSKISHAALRDAIDSDFIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9K4</accession>
    <name>A0A1E3P9K4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_15743</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041300.1">
        <property type="nucleotide sequence ID" value="XM_019180657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9K4"/>
    <dbReference type="STRING" id="683960.A0A1E3P9K4"/>
    <dbReference type="GeneID" id="30197903"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000919">
        <property type="entry name" value="NPR1p Protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF113">
        <property type="entry name" value="NITROGEN PERMEASE REACTIVATOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="219"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="75"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="573" mass="62745" checksum="28C74AC013AA08CE" modified="2017-01-18" version="1" fragment="single">SNSHSYSSSIPYSVPNSGNQDTSIHTNPSSVPRHNGLPSNISMIESINITSPKVSTSDIEPRFVISKQKIAAAHAANSQSRSMSTSHSSGMFSRSRKNSQMDLGSFYNNNYSSQNSPANGHAPPPIEIVTGSPSSSSSVESYSRSRHSSMVDLKRFFKKSGSSPKTNGSSFAMSPIASNFSMGSSSLSRDRSGSMSSSYSNNVSAYTTPQTLPFSKRYGKFGENLGAGAGGSVKLVKRLSDQKVFAVKEFRQRTATETRREYAKKITGEYCIGSTLRHPNIIETVEIAYENDRILQVMEYCDYDLFAIVMSNKMSAHEIDCCFKQILTGIEYLHHMGLAHRDLKLDNCVINSQGIVKLIDFGSAVVFSYPFSKTLIEASGIVGSDPYLSPETLVFSKYDPRPVDIWSAAMIYCCMVLKKFPWKVPKLADNSFKLFCSGRDSDSLSALLTRVPSPKPVEVKPIEKSSGLSNLSKAFSKPLSEVETLVGDSRLLQALPAQARSVIGRMVDLAPACRASIDEVMADPWLKNVATCTVEEHIDGSGISNHIYHKAKNHEHTQVDQSEAHIASLEKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9M5</accession>
    <name>A0A1E3P9M5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_75858</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040859.1">
        <property type="nucleotide sequence ID" value="XM_019184273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9M5"/>
    <dbReference type="GeneID" id="30201519"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="229" mass="25992" checksum="EAD61FF824734303" modified="2017-01-18" version="1">MDSISLLDISSYLKVVVPKGCTIKILWPMQDMKNTYVYSDAYISFENKLEHQTQNDSNLSFNVERDSVVTQKRYYPPKCSHELKVEFDQKLAFYSEETSMDGTRCCIEIIVNKAGVHVKRFKNITLKDNAGEDFDDRNGLGDEDESLKVLFSDDNSSEDSDESLTPSSTNTITTQVNTPAAGYYKLGPNLDIVFRYGYCRSSSRSKETFTCVKSPFSSTNSTRVYLDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9S7</accession>
    <name>A0A1E3P9S7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Actin cytoskeleton-regulatory complex protein PAN1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_87386</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041376.1">
        <property type="nucleotide sequence ID" value="XM_019186107.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P9S7"/>
    <dbReference type="GeneID" id="30203353"/>
    <dbReference type="OrthoDB" id="524326at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF170">
        <property type="entry name" value="DYNAMIN ASSOCIATED PROTEIN 160, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EH" evidence="4">
        <location>
            <begin position="16"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="4">
        <location>
            <begin position="146"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="177"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="4">
        <location>
            <begin position="285"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="3">
        <location>
            <begin position="1392"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="241"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="373"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="873"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="951"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="999"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1150"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="580"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="765"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="373"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="475"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="519"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="532"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="886"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="906"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="963"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1003"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1224"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1302"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1334"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1358"/>
            <end position="1368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1435" mass="154483" checksum="E4BA0AEAF7BFD5E1" modified="2017-01-18" version="1">MATPIHNLRQPLTDQEKHFYDSIFKSLDTESLGIVTGETARSTFEKSGLPPAILGEIWQIADPTNLGFLSQFAFSVALRLIGHVQNGAKPDKSLIDYAGPVAKFQGVSGPNVAPVPALAHSSTGSISSPQQLQPQLTSLPPLTNHDISKFSQLFAKSAPTGIISGDQARNIFLKAKLPTNVLSEIWALADKNNRGQLDRDEFVVAMFLIQGTINGTIRQLPPSVPQHTWDQLKNFQSPITTGGSSTAGVPASSFSPQVTASRPNISRVPSSFANASNDWTISPQKRAQFDSIFEGLDKESRGVLGPNEIAPFLTTSKLSQDVLANIWDLSDIHNTGEFTKTEFAIAMFLVQKKIAGSELPHVIPDSLLPNYYQGASTSPTEQYKQQSVPQPGANQSHSQDHAIPQIPSRDTKPKSSLTDLVDLNDAFSTPSPTIAEAKLPEKDASRTASNSTVKYTPAGGAPRPFVPSSTFGQEIFEKQKAEAPKEVAQPPAPGPVQAKSPEAPVQLKESPVESKFAPQFTGSTQQSSSGQVPTLSTGNRAPSFQTTPSFSQQPTGSLNRSLPRNDDLLADSNPEISGQLSQATADMANLSNQIGSLTNQTSQLHEKRGRAEKELAKITTLKKDIEAKLSKLRVAYEQEVQQTQQVETSLKASKEETEQLRKEASVAEAQYNSVQTQLQSLQSELEESQKENASLKERLGTLNAEHIEVQKQLEKVQGDVRQSKGLAAINSKQLSTTEVSSNGIKAEIAALLASIAELDSHHEGYTKKQAELESLKKEIDTNEQSLAQRRQAHEQSEVDFKAKDEELQSRLQEQQQREAQIQQHEAHIQQLFANLQERQRQLENAENQLQEQQVQYAQRVQEFSNRQIEEASNGYNLSSASREIEPSATPAVPPTTSKEAVIPSDLESSTSATRDLDTASSSKDSNTSKGLLGAAVGAVAGAIGGTALAARSATTESERDNTEFSSPVAETTEQTTSVNSDDYQYEAGRDVNQFNLPIERPESATSSVQNNAPQSVRGDDIEENPTIDSVVETPALASDVLSKDYNAGERHQDAVEATTESPDVGSFEIVDEKSESDLTLRPGTGPEQSADAIESSIPGAWNVDSAEAQQENAEAKDPEPAPSVVDPQLRDASASTKAIHEDAIAAAAAATGATDDEVTAAKTDAPVRSASSIGEEFPPIKELNINESESDDEEFHETSENLPDQTKSPSKHTGGAIGAGVGATAGIAGASALGAGNKDFNFETPFAPSKTPEKRVDELFQDDFEGLEAAKEDTLNDDDEFNQTRDFNEQLDGSNFYGADNFSGQQSQQFSDPAHGQDSNGEWEQIFAGFGNEGQAQQPQEAVSQPPAASSHPYAQNAFGSQSSQQKVIQPEPTHPGRVASSSSSHKHGRIATTPRSLAVQELTGMGFSKEEALAALTKEKWNLEAATNHLLDNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA62</accession>
    <name>A0A1E3PA62_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycosyltransferase family 15 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_13456</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041397.1">
        <property type="nucleotide sequence ID" value="XM_019180574.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA62"/>
    <dbReference type="STRING" id="683960.A0A1E3PA62"/>
    <dbReference type="GeneID" id="30197820"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF7">
        <property type="entry name" value="MANNOSYLTRANSFERASE KTR4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="360" mass="42812" checksum="04797925D6FC7203" modified="2017-01-18" version="1" fragment="single">DVLKQNVQLFDEVMRKPITEPKNLPITRIGDPNYEKANATFVSLVRNSELRGLMSTISQIEETFNKKYQYPYVFLNDKPFSDRFKKGIAQVTSAPVYFEVVDSASWDRPDFIDPVKEKEGVMYLDEKGVGYSKKISYHNMCRYYSMNFYNHERMKQFRYYWRLEPNTNYFCELDYDVFKFMQDNDKVYGFVLNLYDSPDSVRSLWPTTQEFLKKNPQYLNKNAALSWILENEQNPEQTAIANGYSTCHFWSNFEIGDMEFYRGEAYTKWMEYLNENGGFYYERWGDAPVHSLGLGLFATKTQIHWFRDIGYEHFPYNNCPNSPKCKKCKAGMFSKFKNLNNQNCQASWIREMTNEELNIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAJ0</accession>
    <name>A0A1E3PAJ0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Quinate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_24765</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041630.1">
        <property type="nucleotide sequence ID" value="XM_019181055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAJ0"/>
    <dbReference type="STRING" id="683960.A0A1E3PAJ0"/>
    <dbReference type="GeneID" id="30198301"/>
    <dbReference type="OrthoDB" id="508119at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000026">
        <property type="entry name" value="MFS quinate transporter QutD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF34">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0672">Quinate metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="34"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="365"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="407"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="442"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="12">
        <location>
            <begin position="36"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="523"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="539"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ62423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="552" mass="61609" checksum="2EAE3E38706550AC" modified="2017-01-18" version="1">MKTSKVLKSFKLFQKSKVESPIPIPNEIFNLRTYIFAILASFGAIISGYDTGFIGGTVTLESFRHEFGIDKMSSSHATYVNSNIISVFHVGAFFGALGIYPIGELYGRRIALFLSGFFLTFGAAISLISNSQRGLGAIYAGRVLTGVGIGFCSGITPMYVGEISPPSIRGQLTGLWEIAWQIGGLVGYWINLGVNKHVSNPTSQWLIPFAVQIIPSGLFWIGVVFLPESPRWLISKYKRDEAEEKLSYITTLPKDHEFVQFELGKMEEKRELKRKLYGEGIFQQIKTVIFSSRYIKRLILSTSTFILQNGAGINAVTYYSVTVFKSIGLSGADAKLFSTGLFGVIKLVAAVVWLGLIVDNWGRRAAFFWGCIPCGIAMWYLGAYIKVSDPASKLATGNVKLDSGGKGALAMFYIWTFVYGATFNGTCWVYCAELFDQDVRSLVSAINASSNWFWAFIFARFTGNMFNAMGYGVYFLFASCMTVGPLFVFWFFPETKGVPLEVIDHLFEVPAWRGREYALSKIERSSKEDRDEREESTEREDISVKQTKSEHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAP4</accession>
    <name>A0A1E3PAP4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PAN2-PAN3 deadenylation complex catalytic subunit PAN2</fullName>
            <ecNumber evidence="10">3.1.13.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">PAB1P-dependent poly(A)-specific ribonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Poly(A)-nuclease deadenylation complex subunit 2</fullName>
            <shortName evidence="10">PAN deadenylation complex subunit 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PAN2</name>
        <name evidence="13" type="ORF">WICANDRAFT_103259</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein PAB1. PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. May also be involved in post-transcriptional maturation of mRNA poly(A) tails.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 2 metal cations per subunit in the catalytic exonuclease domain.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="10">Positively regulated by the regulatory subunit PAN3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms a heterotrimer with an asymmetric homodimer of the regulatory subunit PAN3 to form the poly(A)-nuclease (PAN) deadenylation complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">Contains a pseudo-UCH domain. This ubiquitin C-terminal hydrolase (UCH)-like or ubiquitin specific protease (USP)-like domain is predicted to be catalytically inactive because it lacks the active site catalytic triad characteristic of thiol proteases, with residues at the equivalent structural positions that are incompatible with catalysis, and it cannot bind ubiquitin. It functions as a structural scaffold for intra- and intermolecular interactions in the complex.</text>
    </comment>
    <comment type="domain">
        <text evidence="10">The linker, or PAN3 interaction domain (PID), between the WD40 repeats and the pseudo-UCH domain mediates interaction with PAN3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the peptidase C19 family. PAN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="10"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041690.1">
        <property type="nucleotide sequence ID" value="XM_019180372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAP4"/>
    <dbReference type="STRING" id="683960.A0A1E3PAP4"/>
    <dbReference type="GeneID" id="30197618"/>
    <dbReference type="OrthoDB" id="16516at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031251">
        <property type="term" value="C:PAN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06143">
        <property type="entry name" value="PAN2_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000028">
        <property type="entry name" value="PAN2-PAN3 deadenylation complex catalytic subunit PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03182">
        <property type="entry name" value="PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030843">
        <property type="entry name" value="PAN2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050785">
        <property type="entry name" value="PAN2-PAN3_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048841">
        <property type="entry name" value="PAN2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028881">
        <property type="entry name" value="PAN2_UCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15728">
        <property type="entry name" value="DEADENYLATION COMPLEX CATALYTIC SUBUNIT PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15728:SF0">
        <property type="entry name" value="PAN2-PAN3 DEADENYLATION COMPLEX CATALYTIC SUBUNIT PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20770">
        <property type="entry name" value="PAN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13423">
        <property type="entry name" value="UCH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="USP" evidence="12">
        <location>
            <begin position="479"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="875"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="877"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="985"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="1036"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ62483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1078" mass="121728" checksum="9FF3BDFD36475D70" modified="2017-01-18" version="1">MEGWNEITRSPVIRPPKSPSTKPATVSCTVFDEQSDIVWVGDSSGFIASYTGDVLSPYTRFAAHRKPVKQLLSHVKGILSIDEDSIRFTNRRGLMIFQLSSKDHPSLARIGTMEFISPNELMVTAESSLLRLDINKSKITQVIPYKHEIVLIASSKRYIALGRTNGMVDIFDPKSNGIVKTFISNSGSLSDIDLQEHTLVTCGFSQRHGSSVLDPLVNVYDLRVLKPLSPIAFPAGASFVRLHPKLASVAFIASKTGQLQVVDMFNPTDVSLYQIDAGPMISKFELSSTGDFLAIVDAYSLLHLWSHNPEHSSMSSKAPLEYATMPEPTPSKVTIDEDFPLNTIGLPYYKETLLSAWPTQTIFRSAGTIPKPIDSSILQSAQIIDGIAVAPYDSEKYGPRNLAKKYVPIITNSEAGKFKSSKISNSPSKKNKTQENLFEFKESEESNSPPPAFRKLEILYSKFGVDDFDFDSYNQTHFTGLETHVDNSYTNSLLQLYRFVPEMFNFLLGNLAIENLDSNSIIRELGYLYDMMVKSFGHHLRPTNFQQTFSSLQDANNLGLIEKENGTISSTARDIGTRIKLFNKFLLDKITKDELKEVAEVSRSLQSYSIFGIEVESTIRSLYTGAMSTDTSVINTLDVFSPLANSFRPSIKNGSQPSILSYIESSLNTVEQRSMLNEMTGHYEAVEVSHTVKRLPPVLSINLHLKNDELKGIRNNSDWLKPTFYTAPSKGRFTVVENITNHVMANAFEEYSLVGFVCEVTGELDKDKHLVTFVQTYNDFLQKNEWYLFNDFLVTAIPHSEVFNLSYWWKTPVTLVYRNTRNLPRFDYAAWNRGLNDEILYRDHFAYGTREGKKIEYELLTREEAPKPGTLIAIDAEFVTLEEEQFEIKSNGLRSMLKPRKQTLARVSVLRGDDGPKMGVPFIDDYVIVEEGIHDYLTSFSGIEPGDLDPKTSDKALVSRETVYRKLWLLLNLGCVFVGHGLTNDFRTINLSVPQRQVRDTAVFFYKGQRILSLRFLAYVLLEKRVQTGNHDSIEDAHTALLLYKEYLTIKESGDLDYVLDEIYKEGQLLKFKPPEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAY0</accession>
    <name>A0A1E3PAY0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_27328</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endomembrane system</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041782.1">
        <property type="nucleotide sequence ID" value="XM_019181358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAY0"/>
    <dbReference type="STRING" id="683960.A0A1E3PAY0"/>
    <dbReference type="GeneID" id="30198604"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="205" mass="23733" checksum="074508626205EB2D" modified="2017-01-18" version="1">MIKVLLIGDAGVGKSALIVRYCDDYFNENESKSTVGVDLKVKLVSVDNKFFKTVLWDTAGQERYRNLIPSLYKGTNGVLLTYDVTDKNSFDGLYHWIKECYDNCDLSRTIFYLVGNKLDQAEKQVNKEDIRKLLEYVKTNHTDFKINAVFEVSAKYSNFVYGLFDNVIKDLVEHRCYFDENKLRSKRSVDLSTARDDQQTNCCSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB37</accession>
    <name>A0A1E3PB37_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SPS-sensor component PTR3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_60681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041830.1">
        <property type="nucleotide sequence ID" value="XM_019183034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB37"/>
    <dbReference type="STRING" id="683960.A0A1E3PB37"/>
    <dbReference type="GeneID" id="30200280"/>
    <dbReference type="OrthoDB" id="5324744at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="188"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="635" mass="70801" checksum="F4070218DE7AAEDE" modified="2017-01-18" version="1">MSTDNHSPSPDTLKDGIIKSITLPISRDEDTNELVYDIVPDAFILSCGCILSKNFALQLSSNSKLSCPICYSSSSLGYDLKPLQNLYYVLYPNEFENNQKKESLLSLFYSVANSINDKTKTSPSKKSTTLVKHLNTKPTTTSTTPSSTSSSINETIPITTSLKQFPTNSTNSITNSSSIIPSLPPPQEQSIISSSPSSSTIPHPKTESPQEQKVYLFVKCFPTYRRHFQYNTHSKFLKTKSKNFINTSISPSSKFFVLLSVKKYEVYSISSDSKTAPILHCCGKSNGEFGLNFENLQKISNEELIIGGNNNNVANNEVIKKKLNDWEHLYCSISDEYLVISGTNGIFKIIDLINQPGKPIYLYISNFPIRCIEISPNSQLIAYGITGKDRILGTEQALVVLHKLNNQEGGTNNSIFTNDPITITFPYRDPINNLSFSSDSNYLSCSTALESRFLTIWVKNYTEPKLVMKSIRSLDTSLESEGITDIQMFPNNQYMIITSASFNAAPIIIDNNISSINGLQTVAQPKMLLKLDEIGSSIHKSAISPRNDTVAVLDKNGSVYLIFATRMDQESKRIFIVDQVSNAFRKRESASLKFSKDGHKLYILDRKGIFYINDFAAGLPQNPEITRCKPLLWGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBC5</accession>
    <name>A0A1E3PBC5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Agmatinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_66552</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041469.1">
        <property type="nucleotide sequence ID" value="XM_019184048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBC5"/>
    <dbReference type="STRING" id="683960.A0A1E3PBC5"/>
    <dbReference type="GeneID" id="30201294"/>
    <dbReference type="OrthoDB" id="288726at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11592">
        <property type="entry name" value="Agmatinase_PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.10:FF:000014">
        <property type="entry name" value="Arginase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005925">
        <property type="entry name" value="Agmatinase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01230">
        <property type="entry name" value="agmatinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133775" description="Agmatinase" evidence="5">
        <location>
            <begin position="18"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="402" mass="45231" checksum="5E2C170B587FDFA4" modified="2017-01-18" version="1">MKLSNLVLLAIATIVTAHKQEHHQQEPESFISEEDATLEQLWGQDWPYSGINTFAHLPHTKCLLDPNETFDIAVVGMPFDTAVTYRSGARFGPRALRAASQRQNSLRGYNFRADINPYDNWAKVIDCGDIPISPMDNGLALKQMTKGYENLLNHKTAGEEAYPKLIMLGGDHSIILPGLRNLYKKYGEISVIHFDAHLDTWSPKKYPSFWHSDQSKFTHGSMLWMAYEEGLLKHKSNVHVGLRSRLTNIEDYEDDDAQGFYRIHADDIMEEGLNRIAEQIIERVPKDGPVYISVDIDVIDPGLAPGTGTPEVGGFLTRELIYLIRKLENLNVVGADLVEVSPAFDHAEITALAGSQIVYELITNMVKKGPLSFKDTVKDARIIQKIEQYGQGILNQVDDMMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBG7</accession>
    <name>A0A1E3PBG7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_88491</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041928.1">
        <property type="nucleotide sequence ID" value="XM_019186155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBG7"/>
    <dbReference type="GeneID" id="30203401"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="505" mass="58032" checksum="6F9993AFBBE79049" modified="2017-01-18" version="1">MAGNYIIINQDSFGNNSRLSQQFTAISPALIGKNFNISNELKVYIKHNPIIIIYIPSYMLNSKELGGLIGHIMYYFPKKFIRVAITDAAFTKHDVYHILRFFIDSINSGNVVGFDYPGIVTLENRIFERIYLLSIGNRFFLDTSILNTYSYYFPDAVNLKHEISGINLDKVKYIMLDVPNLKNMVIHLSDSCYGITTDHYFRNLLSPNLANLEISFQRTKYYVPATKLQLNVSVLLNKFQMLKELKIDAEGAVLEFDASLEVYAMLDSFILQNYFEPFQLETKIFQNIKSIEFENLAGLVHIDALEDCEKLSSLKIHNCPYIISIENLNLNQLKYFTFTSLHTEAPFLKIQNVSLKNVQMLKFVLRCPGLIIIKNIRATSLVSFVYNCRSLNTLNFPSATIFDDGVNSPNLINLKYTVASTRELVELLNNKFVFERINYLIVHNIGPFGKTVQEMKDQIENAFEEDFPPFELTYYQNDEDKLNVSLTGLHRIGCTDRECDSSLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBH7</accession>
    <name>A0A1E3PBH7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">acetyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_60376</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an acyl-CoA + acetyl-CoA = a 3-oxoacyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:21564"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="2.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.16" evidence="10"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041519.1">
        <property type="nucleotide sequence ID" value="XM_019182980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBH7"/>
    <dbReference type="STRING" id="683960.A0A1E3PBH7"/>
    <dbReference type="GeneID" id="30200226"/>
    <dbReference type="OrthoDB" id="5404651at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050215">
        <property type="entry name" value="Thiolase-like_sf_Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853">
        <property type="entry name" value="3-KETOACYL-COA THIOLASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853:SF8">
        <property type="entry name" value="3-KETOACYL-COA THIOLASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="15">
        <location>
            <begin position="26"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="16">
        <location>
            <begin position="300"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="12">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ62312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="420" mass="44614" checksum="30AEC25C503A083B" modified="2017-01-18" version="1">MERLNQLKNHIVSPKSQVTAQRDDDVVVVAAYRTPIAKGLKGSFKDLASDELLYKFLVKFLDTVKVDPSLIEDVACGNVLNLGAGATEHRAACIAAGIPDTAGFLAINRQCSSSLTAVNDIANKIKVGQIKIGLALGAESMSSNYGPRNMAKLSPFLHNHPEGKKCEIPMGITNENIAFNFDVSRKAQDEFAAQSYNKAEAAVANGLFKEEILPIEVDIKLPNEDEDDEDEDEKPAKTKRVLVTTDEGPRKGVTPESLGKIRPAFKKDGTTHAGNASQISDGASGVLLTRRDVAKELGLPILGKYVNFKCVGVPPEIMGVGPAYAIPAVLTAAGLSVDDIDVYEINEAFAAQALYSVHKVGIDPKKVNPRGGAIALGHPLAVTGARQVATIFRELKPGQIGVTSMCIGTGMGAAAVFVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBT0</accession>
    <name>A0A1E3PBT0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_76508</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041544.1">
        <property type="nucleotide sequence ID" value="XM_019184398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBT0"/>
    <dbReference type="STRING" id="683960.A0A1E3PBT0"/>
    <dbReference type="GeneID" id="30201644"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019185">
        <property type="term" value="C:snRNA-activating protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042796">
        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051575">
        <property type="entry name" value="Myb-like_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621:SF1">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="29"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="102"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="102"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="155"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="161"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="241"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="356"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="423"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="369"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="425"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="462"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="574" mass="65267" checksum="66B29D519D9993FE" modified="2017-01-18" version="1">MAEKKKALQINPLDITESLGYTTFRRDSRKPWTKEEDRRLSELVHGKLIELGYEEGIKSLNHNSWDVNQTQLPQEIDWEEIGEKLQTRKPKDCRKRWTNSLDPNLKKGKWTAQEDEKLLASYKIHGSSWQKISLDIPGRTDDQCAKRYIEVLDPNTKDRLRPWNLEEDLELIKKVKLYGTKWRTISLEMKSRPSLTCRNRWRKIVTDVVRGKALPEIKQALDSIQGGFSTLEKINENLERQRQTQMDTPNDELKGNAGVRNHHGDSFERSKSMKSQTNGTIPSLTSMASPSYSGALGESPKPHNTEIDWKYTLKDTSGLTLSNGNISTTDLAKQLIENAKRYDLKISIHQHIHHHYAPTGSLPKGASPVESTTSQNSGNPDDLVMPNRYSHFNYLPPMVQPQLESSNTITTKESDLARLLNPSGHHQQQQHYQHGNVKNGKRKLSGSSSNNSRSKQRPAVSSRHQPSTGFTDSHRKPSFNQDELEDELDFWETMRSLSQVVNNNNSNNIQSSSNMHRLPQTVPPRGNTPNGGANDLGLYYSVFGEDEEDIDDDEFKNDLKDIEGLAGILPFNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC19</accession>
    <name>A0A1E3PC19_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aldehyde dehydrogenase 5, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_25445</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Minor mitochondrial aldehyde dehydrogenase isoform. Plays a role in regulation or biosynthesis of electron transport chain components. Involved in the biosynthesis of acetate during anaerobic growth on glucose.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041634.1">
        <property type="nucleotide sequence ID" value="XM_019181146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC19"/>
    <dbReference type="STRING" id="683960.A0A1E3PC19"/>
    <dbReference type="GeneID" id="30198392"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004030">
        <property type="term" value="F:aldehyde dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019413">
        <property type="term" value="P:acetate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07091">
        <property type="entry name" value="ALDH_F1-2_Ald2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000001">
        <property type="entry name" value="Aldehyde dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000001">
        <property type="entry name" value="Mitochondrial aldehyde dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="7">
        <location>
            <begin position="33"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00053679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00069627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="497" mass="54014" checksum="DEEDF0D503C0862E" modified="2017-01-18" version="1">MSAPVSIKIKLPNNIEYEQPTGLFINNKLVPSVSGKTFKVTNPSNEELIANLHEADKEDVNIAVDAASAAFKKWSVTEPQVRAMALYRLAELVEKNAELLASIESLDNGKALHCSRSDVKLVISYLRSSAGFADKVDGRSINTGEGYFNYTRREPLGVCGQIIPWNFPLMMWAWKIGPALATGNTVVLKPASATPLSALYASFLAVEAGIPEGVINIVPGSGRKCGDAILRHPKIKKIAFTGSTAIGRSVMIAAAESNLKKVTLELGGKSPNIVFDDADVKTTVQNLITGIFFNGGEVCCAGSRIYIQEGIYKEIIEALKTEVEKLKVGDPFDETNYQGAQTTPDQFQEVLDYIQKGKDQGAKVLTGGERIGTKGYFLQPTIFTNVGDDFTVLKEEIFGPVATVSTFKTIDDLVHRANDSEYGLGAGIHTKSLDRALDVSQRLEAGIVWINTYNDFNASVPFGGYKESGIGREMGSEALDNYTQVKAVRVKINRQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC74</accession>
    <name>A0A1E3PC74_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_77119</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042168.1">
        <property type="nucleotide sequence ID" value="XM_019184504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC74"/>
    <dbReference type="STRING" id="683960.A0A1E3PC74"/>
    <dbReference type="GeneID" id="30201750"/>
    <dbReference type="OrthoDB" id="204949at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03759">
        <property type="entry name" value="proteasome_beta_type_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000003">
        <property type="entry name" value="Proteasome subunit beta type-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033811">
        <property type="entry name" value="Proteasome_beta_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF10">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="205" mass="22658" checksum="CFF09DF16BCD9AA2" modified="2017-01-18" version="1">MSDPSSINGGSVVAMVGKDCVAIASDLRLGNQSLGLSNKFEKVYNYGHVYLGLTGLATDAITLSEVFRYKTNLYKLREERQIEPESFANLVSSTLYEKRFGPYFIGPVVAGINSITEKPFICGFDLIGCIDFAKDFIVSGTATEQLYGMCESLYEPNLEPEDLFETISQSLLNAADRDALSGWGAVVYIITKDKVVKRYLKSRQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC81</accession>
    <name>A0A1E3PC81_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Magnesium transporter NIPA8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_61022</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042185.1">
        <property type="nucleotide sequence ID" value="XM_019183098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC81"/>
    <dbReference type="GeneID" id="30200344"/>
    <dbReference type="OrthoDB" id="2504919at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF86">
        <property type="entry name" value="ADR321CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="50829" checksum="25CAD99CE8956279" modified="2017-01-18" version="1">MPASGTIILGCIIGVISSATQSIGLTLQRKSHLLEEIKLDNHRPPYKRPLWRVGFLLFLIANLLGSSIQITTLPLIILSPLQAIGLVFNSICSSIILHEAFTQLSIIGTILVGFGALFIAAFGSIAEPNHSLKELLDLMQRKQFVSWIIISNTIVVLILIWINVIKLIHSKKFQILHERSSSSNIIRPMFDVSIDKFRFVKGLLYGVISGTLSAHSLLLAKSAVEIIINSIVKKNYNNLQNFKSWIIISSFFILAITQLIFLNKGLKNISTSILYPLVFCIYNITNIINGLVFYNQLKVLTKFQSFMIILGTFLVLLGVFALSWRLEELDPKDHSTAFLKRGSVSTERSGLLSPNMIQTNHQIDGSGGDSDQYVSFGSPKGESPIVIDEEPGYISTDEFEPNTRSPIITSPFKYGSLMDNNQSGIAGSTNQKRRSRRVLSYEQNELLNQLKKMPDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCF5</accession>
    <name>A0A1E3PCF5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Porphobilinogen deaminase</fullName>
            <ecNumber evidence="5">2.5.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Hydroxymethylbilane synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Pre-uroporphyrinogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_82571</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="EC" id="2.5.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HMBS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="5"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041774.1">
        <property type="nucleotide sequence ID" value="XM_019185401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCF5"/>
    <dbReference type="STRING" id="683960.A0A1E3PCF5"/>
    <dbReference type="GeneID" id="30202647"/>
    <dbReference type="OrthoDB" id="564646at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00319"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd13645">
        <property type="entry name" value="PBP2_HuPBGD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.40:FF:000002">
        <property type="entry name" value="Porphobilinogen deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000005">
        <property type="entry name" value="Porphobilinogen deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000086">
        <property type="entry name" value="Probable porphobilinogen deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="entry name" value="Porphobilinogen deaminase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022419">
        <property type="entry name" value="Porphobilin_deaminase_cofac_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022418">
        <property type="entry name" value="Porphobilinogen_deaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557:SF0">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03900">
        <property type="entry name" value="Porphobil_deamC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001438">
        <property type="entry name" value="4pyrrol_synth_OHMeBilane_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00533">
        <property type="entry name" value="PORPHOBILINOGEN_DEAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="9" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Porphobilinogen deaminase N-terminal" evidence="13">
        <location>
            <begin position="42"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Porphobilinogen deaminase C-terminal" evidence="14">
        <location>
            <begin position="272"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ62567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="368" mass="40803" checksum="DAB753F457C4826A" modified="2017-01-18" version="1">MSVPKTHANDKNSSVIVDDYSPNKSTPIVATDSRVPYVPDQIFIGGRKSKLAVAQSEIVKSKIKENFPSISCPILALTTLGDQVQNKPLYSFGGKSLWTKELEILLLQDMGEYHQLDLIVHSLKDMPTNLPEEFELGCIIKREDPSDAVLMAKNSPYKSLQELPSGSVVGTSSVRRSAQLKKNFPHLIFESIRGNLQTRISKLDDDSTDFKCIILATAGLTRVGLEHRITQRLDASLMYHAVGQGALGIEIRNNDAIIKPILKTIEDVNATICCYAERALMRTLEGGCSVPIGVWTTYDDETKMLNLKALVISVDGQKHVEDEITLKIDDYKKDSATCGEVLARKLIDLGAKEILDEINFDKINEKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCG5</accession>
    <name>A0A1E3PCG5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_22553</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041784.1">
        <property type="nucleotide sequence ID" value="XM_019180952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCG5"/>
    <dbReference type="STRING" id="683960.A0A1E3PCG5"/>
    <dbReference type="GeneID" id="30198198"/>
    <dbReference type="OrthoDB" id="1875751at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000025">
        <property type="entry name" value="RNA-binding protein Musashi homolog 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032:SF6">
        <property type="entry name" value="RNA-BINDING (RRM_RBD_RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032">
        <property type="entry name" value="RNA-BINDING PROTEIN MUSASHI HOMOLOG RBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="84"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="136" mass="15350" checksum="6F04BEC0CF1044EB" modified="2017-01-18" version="1" fragment="single">KMFIGGLDWDTTEDRLKEYFSKYGEVIDHTIMRESSTGRSRGFGFLTFATSKSVDDVLKTQHVLDGKVIDPKRAIPRDEQDKTGKIFVGGIAPEVRPKEFEEFFGKFGTIIDAQLMLDKDTGRSRGFGFVTFDAPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PDR3</accession>
    <name>A0A1E3PDR3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein MGR2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_19081</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MGR2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042214.1">
        <property type="nucleotide sequence ID" value="XM_019180816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PDR3"/>
    <dbReference type="STRING" id="683960.A0A1E3PDR3"/>
    <dbReference type="GeneID" id="30198062"/>
    <dbReference type="OrthoDB" id="5409308at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018450">
        <property type="entry name" value="Romo1/Mgr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28525">
        <property type="entry name" value="REACTIVE OXYGEN SPECIES MODULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28525:SF1">
        <property type="entry name" value="REACTIVE OXYGEN SPECIES MODULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10247">
        <property type="entry name" value="Romo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01378">
        <property type="entry name" value="Romo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ63007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="8366" checksum="416D34FC62BBD189" modified="2017-01-18" version="1" fragment="single">MPPVQQVQYGQQPSNFEKFKMGAMMGTTVGVVVGVLFGGFSILTNGAGPNGVMRTLGQYIGGSAATFGLFMSVGSVIRSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUP0</accession>
    <name>A0A1E3NUP0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phosphatidylethanolamine N-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_14677</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036118.1">
        <property type="nucleotide sequence ID" value="XM_019180618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUP0"/>
    <dbReference type="STRING" id="683960.A0A1E3NUP0"/>
    <dbReference type="GeneID" id="30197864"/>
    <dbReference type="OrthoDB" id="4583at2759"/>
    <dbReference type="UniPathway" id="UPA00753"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004608">
        <property type="term" value="F:phosphatidylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007318">
        <property type="entry name" value="Phopholipid_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016219">
        <property type="entry name" value="Phosphatid-EA_MeTrfase_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32138">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32138:SF0">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04191">
        <property type="entry name" value="PEMT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51598">
        <property type="entry name" value="SAM_CHO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="347"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="432"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ56911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="722" mass="83146" checksum="3D9A5F1C5C6EC060" modified="2017-01-18" version="1" fragment="single">LKESKNYKSDKRPVGKTPDGTTFEVPETHDMVRSLFDPTLRKSYAEILICLCLASNFLIYRWLETKSDRIVGFIALYAFWRLAYNFGIGVILYFQSNHNTLVEFAKRHSLFKSSNKTLLQKLANLEIKSKMGNTYKPESYPIEFNTWLLFRQFVDLILMQDFTTYMFLVYTCGQGNTLSQSTFLNTTRIVLGSLLIGFNFWVKVDAHRVVKDYAWYWGDFFFLEENELIFDGVFNLSPHPMYSIGYLGYYGFALITKSYIVLIVSLAAHALQFVFLTWVENPHIEKIYGSETVNNQASEEFESNESYLKPLIVFSNFNPIRISDYLAALLVIYTIVAPFVIQPKNELWTRIFFAAAVIIKLGQSFGVSLILHKQSVDKSWTKLYLKYGLDNVAAYSNWQVLYNLSLVLSYSSLISVVVRELINGQYKLGNWLLLRFIIGGLMVLLQVWTSYSIYESIGEFGWFYGDFFLPKLSNRQLTNSGIYRYLNDPERLLGISGIWGATLVTYSPFVFVLAMLWTAHITIFITFVEKPHMVKVYGEQQVSKNISGVSLTLKQMMPEKFNNTIDSFYNKTFNKVDGYIKKKRSQSETVNRKHVKPILHQHNQLSSEYSIELLNLEIDSTVEVGKPIRAKWTAPKFHSKKDWIGLYKVISTGSSRRQTSISSSGRWIGVNTVTGIVEYSGDLLNFEKGVYELRYHANSSHKVLSISHPFEIVVPTIKVEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUY0</accession>
    <name>A0A1E3NUY0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="5">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_36212</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036145.1">
        <property type="nucleotide sequence ID" value="XM_019182407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUY0"/>
    <dbReference type="STRING" id="683960.A0A1E3NUY0"/>
    <dbReference type="ESTHER" id="hanan-h6uwn4">
        <property type="family name" value="Fungal_carboxylesterase_lipase"/>
    </dbReference>
    <dbReference type="GeneID" id="30199653"/>
    <dbReference type="OrthoDB" id="3977998at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="6">
        <location>
            <begin position="9"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ56938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="553" mass="61941" checksum="2BA63F0FDD0166B4" modified="2017-01-18" version="1">MKRDDLSSSPIVHVKNGSIQGQVIERFNQDAYLGIPYAQPPLGSLRFVSPQSLNQSWSDVKTFDTYGPSCFASGSNQSSQLPQSEDCLKLNVVKPRGYDNSSVKLPVAVWIHGGSFMDGSSTRPSYNLSYIVENSVDIGKPFIGVSLNYRLTGFGFLRSREVEYKGYTNVGLRDQIKAIQWVHENIEAFGGDPNHLVIWGESAGGYSVGKLLNSGQLGDYVKGAIMESGTSMFADYFADTLVTAEDDFAKLSNHFNCSEAPSSFECLQHVDAHELNHVFNETNKIIKGGFHQAYVDGDIIPKSSYLSLQAGEYKKIPLLLGSNSDEGYDFINTTLNTTEEFKAALAKDFPYLTNSSIDKLDELYPLGDASVSSPLDPTYNNTPIEYPSSYGKQAPRMSVLWGDLLFIAGARKTAEIFSRDGVPVHKYRHNIPDNETFNEPFIGAKHFTEIVYVFDNSDEPEHSTLGEIMFDDPRIPNVSKSISKMWASFITDLDPNLNQDQLNIDDVPKWPEYRDGAQNIVFDINGFYLEDDTWRKEQFKFIESIEVEQLEMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV24</accession>
    <name>A0A1E3NV24_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_85914</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036141.1">
        <property type="nucleotide sequence ID" value="XM_019185970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV24"/>
    <dbReference type="STRING" id="683960.A0A1E3NV24"/>
    <dbReference type="GeneID" id="30203216"/>
    <dbReference type="OrthoDB" id="2153661at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="326"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="350"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="38"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ56934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="466" mass="51530" checksum="C860C88B519A5F0A" modified="2017-01-18" version="1">MTLKTDNIEKVEYEVNSTEIQKSNSSEQISDNEAQEGGIFTCGAAFLSDGYQQGVMTMANAVLSKSFKGYNTKYKTMVSNSILVANIIGQIAFGFITDRVGRKQAFIYTTVFIIVGAIVCACASGSTEQRLFWMLIIGRGILGCGVGGEYPTSASASIETANEKMSIKNRVTPFIMSTNFFVAAGIPIATIVFLIVHEIWGPNHLNGIWRTCFAIGAVIPISIFYFRWKLDHANSYKTNAIKRKVPYKLVWKKYWKEFVSTAAMWFFMDMIVYPNNIFSTSILAVAIPKATLKRTGEWQLFLSSFSIFGVFASCIGLRFITRRQAIISGFIGYGIISFIVGGAFEKFSEIPALLIIFYAFLNMIINFGPASLQSVVSSESFPTAVRGTIYGISAGIGKAGAAVGTEVFTPLQVNYGKRYTFFLSGGISFLDYGDRTLDFLDEEFNQYLKENGWEGSIGEKENAKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV39</accession>
    <name>A0A1E3NV39_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_36132</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036203.1">
        <property type="nucleotide sequence ID" value="XM_019182399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV39"/>
    <dbReference type="STRING" id="683960.A0A1E3NV39"/>
    <dbReference type="GeneID" id="30199645"/>
    <dbReference type="OrthoDB" id="1929172at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133530" description="GOLD domain-containing protein" evidence="10">
        <location>
            <begin position="25"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="175"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="11">
        <location>
            <begin position="37"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ56996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="209" mass="24098" checksum="73C292561937F901" modified="2017-01-18" version="1">MLKYSQLLWSSLLVVLCCLSFVKADQKVSINIPSFAKECIYNNLDNDDHALIINYQVLQGGNFELDFEITSPKGETIIKQTNEKYADFLLKTFGLGEYTFCFSNPYNTLKKVEFSIEYENKENKERLSNDKDSIVSEHSLSEIDRNVVKIAKIMDYLRAREWRNMSTVQSTESRMVYLSIGIIVLMVAISFGQATIVQFLFEGRTKTYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV89</accession>
    <name>A0A1E3NV89_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2'-phosphotransferase</fullName>
            <ecNumber evidence="3">2.7.1.160</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_36566</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2'-phospho-[ligated tRNA] + NAD(+) = mature tRNA + ADP-alpha-D-ribose 1'',2''-cyclic phosphate + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:23324"/>
            <dbReference type="Rhea" id="RHEA-COMP:11106"/>
            <dbReference type="Rhea" id="RHEA-COMP:11107"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:76596"/>
            <dbReference type="ChEBI" id="CHEBI:82883"/>
            <dbReference type="ChEBI" id="CHEBI:85027"/>
            <dbReference type="EC" id="2.7.1.160"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KptA/TPT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.160" evidence="3"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036328.1">
        <property type="nucleotide sequence ID" value="XM_019182446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV89"/>
    <dbReference type="STRING" id="683960.A0A1E3NV89"/>
    <dbReference type="GeneID" id="30199692"/>
    <dbReference type="OrthoDB" id="419694at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000215">
        <property type="term" value="F:tRNA 2'-phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.970:FF:000002">
        <property type="entry name" value="Tpt1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.170.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.970">
        <property type="entry name" value="RNA 2'-phosphotransferase, Tpt1/KptA family, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002745">
        <property type="entry name" value="Ptrans_KptA/Tpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042081">
        <property type="entry name" value="RNA_2'-PTrans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042080">
        <property type="entry name" value="RNA_2'-PTrans_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12684">
        <property type="entry name" value="PUTATIVE PHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12684:SF2">
        <property type="entry name" value="TRNA 2'-PHOSPHOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01885">
        <property type="entry name" value="PTS_2-RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="209" mass="23808" checksum="FF9CB253E022777E" modified="2017-01-18" version="1">MSEKRDIQISKALSYLLRHGAAKEKLPMDEQGYVKLSTILSNNRIKTHRATKQDIERIVETNNKKRFTLISRDDGIYICANQGHSLKTINNDNLQKLEPKDFPSQLIHGTTLPKLKLILESGGLSKMNRNHIHFTSNLTNDDANGVVTGIRYNSNILIYLDITKLQNQDEIQFYKSLNDVYLTPGDSNGVLSKEFFFKIVDRKTGQSVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVJ6</accession>
    <name>A0A1E3NVJ6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosome-releasing factor 2, mitochondrial</fullName>
            <shortName evidence="5">RRF2mt</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Elongation factor G 2, mitochondrial</fullName>
            <shortName evidence="5">EF-G2mt</shortName>
            <shortName evidence="5">mEF-G 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">MEF2</name>
        <name evidence="7" type="ORF">WICANDRAFT_97638</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Not involved in the GTP-dependent ribosomal translocation step during translation elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036340.1">
        <property type="nucleotide sequence ID" value="XM_019186601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVJ6"/>
    <dbReference type="STRING" id="683960.A0A1E3NVJ6"/>
    <dbReference type="GeneID" id="30203847"/>
    <dbReference type="OrthoDB" id="198619at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051881">
        <property type="term" value="P:regulation of mitochondrial membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01886">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03713">
        <property type="entry name" value="EFG_mtEFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000514">
        <property type="entry name" value="Ribosome-releasing factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03059">
        <property type="entry name" value="mEF_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030851">
        <property type="entry name" value="EFG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43261:SF1">
        <property type="entry name" value="RIBOSOME-RELEASING FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43261">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="6">
        <location>
            <begin position="24"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="33"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="97"/>
            <end position="101"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="149"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="761" mass="84914" checksum="963EAE7A53FA3841" modified="2017-01-18" version="1">MWKGSFVRLFHTSRVVRAPSHPLSKIRNIGIIAHIDAGKTTTTERMLYYSGITSRIGNVDQGDTVTDFLPAERERGITIQSAAITIPWNKHKINLIDTPGHADFGFEVIRSLKVLDGAVTILDGVAGVEAQTEKVWKQSKTIPKMFFINKMDREGAGFSRTVKEIIAKLGTRVLLLNVPYFVEMENREKKFVGVLDIIDKKLLRWIDQDNIKVTDVEGETYEEVARCRESLIETLGEIDDAVIDSFLECEDYMKVPADVIRKAVRKATLSNYAVPVLTGASFKNIGVQPLMDAVNYYLPSPIECQPEVSCPVVKTDEGFVMNQNKYLAVAMAFKVITDPIRGVMVFVRVYSGKLKNHATVYVNGEKFKIGKLLVMHANVPQDVTEISAGNIGVIVSDKLRTGDTILSNIQNKKLQNKELGIVVNPIIVPPPVFTISLEPRTSGDKRSMDEALEIILREDPSLKVTVDEENGQTLLAGMGELHLEIARDRLRDLKAKIEIGQIMVSYKETVLEKLSVSKESPEHEFKISMALEPKPELFEENPNVVRVNLGDNNVLEVPVEPENWEKVMPYQSCINAFISGSISSIQRGPILKLPLNSVLIKVTSFDMPPDSQNVTLWITLVRQCILELLQDETRFVGLEPVMNVSVDVGAEDIGSVIQDLSSARQGIISSIDEGGDNVEFAKFKDISESQYVPYDPTLEFTKTRDGFSGDQTVHAVVPLREMIGYLPKLRSLTQGRATYHMEFKGMNPITSDRLVEISSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVL1</accession>
    <name>A0A1E3NVL1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ammonia transport outward protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_36304</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036351.1">
        <property type="nucleotide sequence ID" value="XM_019182419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVL1"/>
    <dbReference type="STRING" id="683960.A0A1E3NVL1"/>
    <dbReference type="GeneID" id="30199665"/>
    <dbReference type="OrthoDB" id="3648309at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015123">
        <property type="term" value="F:acetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051633">
        <property type="entry name" value="AceTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047622">
        <property type="entry name" value="GPR1_FUN34_YAAH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038013">
        <property type="entry name" value="AceTr_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123:SF1">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01114">
        <property type="entry name" value="GPR1_FUN34_YAAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="30248" checksum="2165BAE8B0ED04E5" modified="2017-01-18" version="1">MSQYNTHNNPDLEKQISNSDNNLDNQTTTYNDPQDQQEFSRITHQGEYINIGRTKVLKSELIEAFGGTLQPGVSVPSTHKFANPAPLGLCGFALTTFVLSMYNAQAMGIHTPNVVVGLAFFYGGLVQLLAGMWEIALENTFGGTALSSYGGFWLSFAAIHIKWFGIKEAYTDPVEFRNAVSFFLLGWTIFTFGLMLCTIKSTVGFFGLFFFLEITFLLLTIGDFTGRTGVTRAAGVFGVITAILAWYNAFAGVATRENSYITVKAIQMPKPKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVS5</accession>
    <name>A0A1E3NVS5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_98086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035999.1">
        <property type="nucleotide sequence ID" value="XM_019186627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVS5"/>
    <dbReference type="STRING" id="683960.A0A1E3NVS5"/>
    <dbReference type="GeneID" id="30203873"/>
    <dbReference type="OrthoDB" id="3366546at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050656">
        <property type="entry name" value="PINX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149:SF26">
        <property type="entry name" value="PROTEIN TMA23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ56792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="26570" checksum="2D909D9AA843219A" modified="2017-01-18" version="1">MDSKGYLESFGWRNGEALKKGGIKKPILVKHKKDKKGIGHQPNGSADAWWERLFDGQLKGLDVNQDSANGISFKQEKEVIATGIRQQDSPLYKMFVRGETLQGTTGKVILTKSTIKEDGTNSIVDTIKTSFNDSSKKTSTPETIPSDSTIEKTKKDKRSKKDKKSKKEASEKKEKKEKKSKDKKEKKMKSVASSEEPRSESKKSKKDKSGKDSKKTSKKRKLDEDITKSSNLKKVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVU8</accession>
    <name>A0A1E3NVU8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ran GTPase-activating protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_65526</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036475.1">
        <property type="nucleotide sequence ID" value="XM_019183929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVU8"/>
    <dbReference type="STRING" id="683960.A0A1E3NVU8"/>
    <dbReference type="GeneID" id="30201175"/>
    <dbReference type="OrthoDB" id="184583at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006404">
        <property type="term" value="P:RNA import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00116">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF12">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="351"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="415" mass="46718" checksum="1DE31F200894BDEA" modified="2017-01-18" version="1">MASLDFTPEYKDEEVYSLAGKSLKFDTKEDIEPYLKELNALKNVKKLDFSGNTIGIDASKHLADSIDLHRDTLEEVNFADLFTGRLKDEIPKSLEFLLPALAKAPNLHIINLSDNAFGLQTIEPVEDFIAKAVYLQHLILSNNGMGPFAGERIGKSLFKLSLAKQKLGQPSLKTFICGRNRLENGSTTYLSIGLKAHKDLQVVKLYQNGIRPQGIAKLILQGLKHNTKLTILDLQDNTITKKSAKVLADNLSIWKELKELNVNDCLLNPLGSLELVKAFNAGESLQGFKTLRLQYNELDSKALEILKDVIANKLPKLESLELNGNRFEEESSIVDEINAIFEERGFGELDELDDLEELDSEDEEEETESEEEAEEEEIEAEVLEKELEQEQENYTVDNDNKQVQDIAAELEKTHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVZ4</accession>
    <name>A0A1E3NVZ4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">KOW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_85580</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036586.1">
        <property type="nucleotide sequence ID" value="XM_019185912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVZ4"/>
    <dbReference type="STRING" id="683960.A0A1E3NVZ4"/>
    <dbReference type="GeneID" id="30203158"/>
    <dbReference type="OrthoDB" id="1875589at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23702">
        <property type="entry name" value="eL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000030">
        <property type="entry name" value="60S ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039660">
        <property type="entry name" value="Ribosomal_eL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002784">
        <property type="entry name" value="Ribosomal_eL14_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01929">
        <property type="entry name" value="Ribosomal_L14e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="KOW" evidence="7">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="138" mass="15210" checksum="166DBFB736FF7C61" modified="2017-01-18" version="1">MSTETSVKASNWRLVEVGRVVLVNKGQYAGKLATIVEIIDHKRALVDGPTTGVPRQSISLGHVVLTPLSYSLPRGSRTSTVAKKFEAAGVESKWSSSSWAKKIAQRERRRALTDFERFQVLVLKKQKGYAVKKAVSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW42</accession>
    <name>A0A1E3NW42_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_98045</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036542.1">
        <property type="nucleotide sequence ID" value="XM_019186623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW42"/>
    <dbReference type="STRING" id="683960.A0A1E3NW42"/>
    <dbReference type="GeneID" id="30203869"/>
    <dbReference type="OrthoDB" id="440760at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000312">
        <property type="entry name" value="Exosome complex component CSL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039771">
        <property type="entry name" value="Csl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048626">
        <property type="entry name" value="CSL4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019495">
        <property type="entry name" value="EXOSC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE CSL4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT CSL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21551">
        <property type="entry name" value="CSL4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10447">
        <property type="entry name" value="EXOSC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0271">Exosome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exosome complex component CSL4 N-terminal" evidence="5">
        <location>
            <begin position="11"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Exosome complex component CSL4 C-terminal" evidence="4">
        <location>
            <begin position="119"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF21551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ57335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="280" mass="30112" checksum="923230C13C438F59" modified="2017-01-18" version="1">MSLEIPSFTVPGQPICPSLKSTNDPKLIHKFEPGNGAILQEVEVNNKKSSIISATLVGKVKVEEIPDKKEEAEEDKEDSKDIDLQVKRFKVSVIPTSSNDYDDYGKTSTETDSNFATNLPKEGDIVITRVTKLNLKQAFVEILAVEGAGNVLTDSGIGSNGHGVIAAGGGSGASTFSIHQASSDLGETFKGIIRSQDVRSTERDRVKIIESFKPGDIVRAQILSLGDGTNYYLTTARNDLGVVFAKAQNGAGGLMYAIDWQTMVCAKTGELEPRKCAKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWK4</accession>
    <name>A0A1E3NWK4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_107307</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036144.1">
        <property type="nucleotide sequence ID" value="XM_019180497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWK4"/>
    <dbReference type="STRING" id="683960.A0A1E3NWK4"/>
    <dbReference type="GeneID" id="30197743"/>
    <dbReference type="OrthoDB" id="3437960at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000064">
        <property type="entry name" value="Early growth response protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF120">
        <property type="entry name" value="KRUPPEL-LIKE FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="15"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ56937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="266" mass="30739" checksum="EA05E0C72B14DE0F" modified="2017-01-18" version="1">MPAISKKPATRPKKYHCDFEGCLKAYSRPCLLEQHIRTHSNERPFKCGQCGKAFFRDSHLKVHLWTHSSDKPLRCEICSKGFVTSQQLGRHVKTHSIDEFKCPYECDESFPTNDKLSEHMLSTHIMSDFVDANTHQMKTDETLNMLRDQLDYSGIRGIPTFEVYTSPTPKAGDIDDGSNGYKTVSSTSTDAYDNLNLSKGKTFWDNWDDHHCKEPNCKGYPKYNSFQDLIYHYDEYHSFIPESLFEAFESNPEESSTGRFSNEWNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX62</accession>
    <name>A0A1E3NX62_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-1,4 glucan phosphorylase</fullName>
            <ecNumber evidence="10">2.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_35719</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + phosphate = [(1-&gt;4)-alpha-D-glucosyl](n-1) + alpha-D-glucose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:41732"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9586"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.1" evidence="10"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036892.1">
        <property type="nucleotide sequence ID" value="XM_019182345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX62"/>
    <dbReference type="STRING" id="683960.A0A1E3NX62"/>
    <dbReference type="GeneID" id="30199591"/>
    <dbReference type="OrthoDB" id="9215500at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04300">
        <property type="entry name" value="GT35_Glycogen_Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000002">
        <property type="entry name" value="Alpha-1,4 glucan phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000003">
        <property type="entry name" value="Alpha-1,4 glucan phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011833">
        <property type="entry name" value="Glycg_phsphrylas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035090">
        <property type="entry name" value="Pyridoxal_P_attach_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02093">
        <property type="entry name" value="P_ylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468:SF3">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE, LIVER FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000460">
        <property type="entry name" value="Pprylas_GlgP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00102">
        <property type="entry name" value="PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10 12" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000460-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ57685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="867" mass="99237" checksum="D7C9BF7EB58609CF" modified="2017-01-18" version="1">MSSGYDFHPSETNNDLPRPKHIRRFTGFSPAEIKTIDSVIPLKSRETWKKFAVENFSTKETFEKDFVRHVETTLARSLYNCDELAAYQATSNSIRDNLITDWNKTQQKQTTKDQKRVYYLSLEFLMGRALDNALINTNTRELVSDSVDELGFNLEDLIQQEPDAALGNGGLGRLAACFVDSLSTGNYPAWGYGLRYQYGIFAQKIIDGYQVETPDYWLKFSNPWEIPRKEIQIPVDFYGYVEHSSDDNGVKKSNWIGGERVLAVAYDFPTPGYKTNNVNNLRLWSAEPTTEFDFSKFNAGDYQNSVAGQQRAESITAVLYPNDNFDSGKELRLKQQYFWVSASLHDIIRRFKKTKRPWSEFSDQIAIQLNDTHPTLAIVELQRVLVDLEGLSWDESWEIVTSTFGYTNHTVMTEALEKWPVELFQKLLPRHLEIIYDINLFFLQFVEKKFPKDRDLLSRVSIIEESSPRQVRMAYLAIIGSHKVNGVAELHSELIRTTIFKDFVKIYGSSKFTNVTNGITPRRWLRQANPKLAELIASKLGGDHYLSNLNELKNLEQFAEDEEFQKAWFDIKLDNKKRLSALIKTLTGVEIKNPNVLFDIQVKRIHEYKRQQLNILGVISRYIKIKNSSKEERAKILPKVVIFGGKSAPGYYAAKLIIKLINSVGEVINNDEDVGDLLKAVFIPDYNVSKAEQIIPASDLSEHISTAGTEASGTSNMKFVMNGGLIIGTVDGANVEITREIGEDNIFLFGHLAENVEDIRYEHRYGGYKIPDSLEQVFKLIESGKFGDYNEYRSLVDSIKYHGDHYLVGDDFESYLQAHELVDQAYLDQKEWLKKSILSVANSGFFSSDRAIDEYAESIWNIESIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX84</accession>
    <name>A0A1E3NX84_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_17855</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ZNF593/BUD20 C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036404.1">
        <property type="nucleotide sequence ID" value="XM_019180762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX84"/>
    <dbReference type="STRING" id="683960.A0A1E3NX84"/>
    <dbReference type="GeneID" id="30198008"/>
    <dbReference type="OrthoDB" id="24683at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000299">
        <property type="entry name" value="Zinc finger protein 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051879">
        <property type="entry name" value="C2H2-ZF_Maturation_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46095">
        <property type="entry name" value="ZINC FINGER PROTEIN 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46095:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ57197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="122" mass="14336" checksum="DA88D77B127E6667" modified="2017-01-18" version="1" fragment="single">MGRYSVKRYKTKRRTRDLDLIYNDLATPESILKLKDAPLDETKPGMGQFYCIHCAKYYENDQAIQAHYKSKIHKRRVKELKARPYTNLESEAASGTNLQSFLQSVEKHKARMAMEEQHKDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXB8</accession>
    <name>A0A1E3NXB8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_80951</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036827.1">
        <property type="nucleotide sequence ID" value="XM_019185153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXB8"/>
    <dbReference type="GeneID" id="30202399"/>
    <dbReference type="OrthoDB" id="10662171at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="241" mass="27504" checksum="85D133FD8545851A" modified="2017-01-18" version="1">MFQSNFNSNDAKDDWINEIDEDDDDDDTFEYLYPTSARSQSGPSKTSTSIKKGAQKVDNVPNKSLSKPPAETPTSSSQSPKLLRVYPPRQQSSYATNCGYPFIKRGKFKGFYDDRRYRCAFSNFDINRCNHSNCYCLHTPIVPSPLKYQRLDFMSTIDEADGDIDEEHTDNNGDDARQDEQNSNDSRSLSSQFKKNRYKLSRVKHRSDDPKSNHEKSPFNASDAGSVSEKLSKQHQLDIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXE0</accession>
    <name>A0A1E3NXE0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Anaphase-promoting complex subunit 4 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_85853</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036972.1">
        <property type="nucleotide sequence ID" value="XM_019185956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXE0"/>
    <dbReference type="STRING" id="683960.A0A1E3NXE0"/>
    <dbReference type="GeneID" id="30203202"/>
    <dbReference type="OrthoDB" id="25131at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039328">
        <property type="entry name" value="WDR89"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22889">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22889:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="155"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="296"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="340"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="386"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="48667" checksum="D1D434D4587FA44C" modified="2017-01-18" version="1">MAAFNVSQQAPTSTYHEINSGYCDNWVLDLVPSKNHGLVSSLSDGSLKLYPLNANQDRNFAPIRTIKAHQSSINSARSVDGENLIGTAAEDSIKIFDLRNNDDKPVAIFNNSRNIPFLSLDFKNNYVAGGTELKQADAELHIWDFRTKDIVRSFIDSHHDDITEIKFHPTDDSILLSGSTDGYVNIYDLSIPDEDDALHQVINFASIHSAGWLSPKRIYTLSHMETFAIHELNDKSDESHEPKPQDFGDVRELWDCEYVVDVYPGFIATGANSKSHAKLYPFINEQINLETPIKLNGGHGEEVIRAVYCPPASNLIYTGGEDGNLRIWKSDVQLNAPESFFGSASTEQNEPKEDVEMGDLEDDSESTSGEHKEKKSKKEKGEKKDKKDKKEKKEKKEKKDKKEKKDKKDKKHKHKEEKKSKKEHRFRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXG8</accession>
    <name>A0A1E3NXG8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_64044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037098.1">
        <property type="nucleotide sequence ID" value="XM_019183660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXG8"/>
    <dbReference type="GeneID" id="30200906"/>
    <dbReference type="OrthoDB" id="4013061at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="183" mass="20422" checksum="A69227A3E97DE2AC" modified="2017-01-18" version="1">MSSSSSKSNKQAALSLLQSNNNNNDQPLIDEDHLSEIDSPNLLATSVENNSLLSSNSTISLLSLNKPSMSRTTSFSNPLMYQRVQFRRYSNNNGNGNLERGGINIPQPQSLRIDPVPETPNLDPISIQNSPSNFWLNKPMIKRQSSITQNVDSPFLYPVRDLDGNVTPLILSPNRNELKDDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXI1</accession>
    <name>A0A1E3NXI1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]</fullName>
            <ecNumber evidence="8">1.5.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Saccharopine reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_80022</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-saccharopine + NADP(+) + H2O = (S)-2-amino-6-oxohexanoate + L-glutamate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57951"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.10" evidence="8"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037060.1">
        <property type="nucleotide sequence ID" value="XM_019184987.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXI1"/>
    <dbReference type="STRING" id="683960.A0A1E3NXI1"/>
    <dbReference type="GeneID" id="30202233"/>
    <dbReference type="OrthoDB" id="10059875at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004755">
        <property type="term" value="F:saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000008">
        <property type="entry name" value="Alpha-aminoadipic semialdehyde synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000072">
        <property type="entry name" value="Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1870.10:FF:000002">
        <property type="entry name" value="Saccharopine dehydrogenase Lys9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1870.10">
        <property type="entry name" value="Domain 3, Saccharopine reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032095">
        <property type="entry name" value="Sacchrp_dh-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF22">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16653">
        <property type="entry name" value="Sacchrp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="11">
        <location>
            <begin position="5"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase-like C-terminal" evidence="12">
        <location>
            <begin position="122"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ57853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="449" mass="49395" checksum="78CA2DD3C5A12187" modified="2017-01-18" version="1">MVKKVLLLGSGFVAKPTVDILAKTEGVQVTVACRTLEKAQSLASDVATAISLDVSKESELDAAVSDHDLIISLIPYIYHATVVKSAIKHKKNVLTTSYISPALKELEDQINEAGILVFNEIGLDPGLDHLYAVKTIDEVHKAGGKIKSFISYCGGLAAPEDANNPLGYKFSWSARGVLLALRNSATFYKDGKKVEVDGPELMGYTAPYKINEFTEKGLVAYPNRDSTVFRELYEIPEAETVIRGTLRFEGFPEFIKVLVDLGLLKDDENEIFTQKLSWKDALAKYLGAESSKEEDLIKSIESKTKFADEDAKKKIIDGFNWFGLLSDEEIDPKGNPLDTLCSRFEKLLQFEKGERDLVILQHKFGIENKDGSEETRTSTLVDYGAPTGSGYSSMAKLVGTPAAVASQLILSGKLPVQKGLIAPYSPEINDPIIKILKDEYKIELTEKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY51</accession>
    <name>A0A1E3NY51_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_35634</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036714.1">
        <property type="nucleotide sequence ID" value="XM_019182335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY51"/>
    <dbReference type="STRING" id="683960.A0A1E3NY51"/>
    <dbReference type="GeneID" id="30199581"/>
    <dbReference type="OrthoDB" id="5841748at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF72">
        <property type="entry name" value="ZINC METALLOPROTEASE TRE2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="4">
        <location>
            <begin position="357"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="3">
        <location>
            <begin position="589"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="717" mass="81758" checksum="AB33E6683ECBC0EE" modified="2017-01-18" version="1">MEVDEPGSSSRLSGSLSRARFAGQRIASQFNSKIITPVQRILDPVAEGYSYVSRKFEAYLSKLGNPLILKRFAYVIFISAIIYFITIAGLFPTDNSRQFGSFSDRGLLNNFIKDSIDLKSMEESLEYLSSIPHHAGTSGDLVLARYVESFYREQNLKLVDFDELRTTLNFPNKTTLSYGDVTIDITDQFNPLSKDEKVTGNLIYANYGRKLDFEKLQSKKVHLEDSIAIIKYGKIPTSSQILLADTFGCKGVLFISENDDYPDTYERRSVGIPEFYLGDPLSPGWSSSQLPNRISMKDASIAKIPSAPLTRNQAKPLLESLKSGVKFDDEQFSGDGQTFEVTLDNHVVFTTDHGIWNVLGKIEGKEQSDKALIIGAQRDSPCNGAIFPNTGTTVLLELVQVFMKLKRNFNWTPLRSIYFISFDASEYNFAGVSELIEGKTREIRKETTAYIDLSEAVAGDKLDIALNPIFKTLFSQYQEEYGFNLHSLQEYKNYVPFITEGVPVIDFGYKGLKYPKYTCQDTFEEFRKIGDKDFSKHAKLVELISKVALNIIEDPLLPFDVTEYVEGLNDIFKDLQRYAHDVDAGHKLRFDPMIEGILKFKRVGKELELWIKAWKDIVEEDGGNEPSLLAVHRWNWNSKLNQIEKLFLVDLGLPKRRWYKNLIFGPQFWKPEEGDYEWWSFPSIRDAIFDHDWNLAQQEIDRVGMALSEGANQFIQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY57</accession>
    <name>A0A1E3NY57_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ferric-chelate reductase (NADPH)</fullName>
            <ecNumber evidence="4">1.16.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">WICANDRAFT_33676</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>2 a Fe(II)-siderophore + NADP(+) + H(+) = 2 a Fe(III)-siderophore + NADPH</text>
            <dbReference type="Rhea" id="RHEA:28795"/>
            <dbReference type="Rhea" id="RHEA-COMP:11342"/>
            <dbReference type="Rhea" id="RHEA-COMP:11344"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.9" evidence="4"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037240.1">
        <property type="nucleotide sequence ID" value="XM_019182104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY57"/>
    <dbReference type="STRING" id="683960.A0A1E3NY57"/>
    <dbReference type="GeneID" id="30199350"/>
    <dbReference type="OrthoDB" id="17725at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF23">
        <property type="entry name" value="FERRIC-CHELATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-0408">Iron</keyword>
    <keyword evidence="17 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="20">
        <location>
            <begin position="315"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ58033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="593" mass="67661" checksum="9ED7A883B98784DA" modified="2017-01-18" version="1">MTVIYKGLDCLADKDDPEYTKLKTISQSGVNAWAYQKNYGYYVLYFGAVLIFLMIIKKIWFTFNDYSYRNTKIGGGKYNLSSLNKFIGITRLISYKKLPIILSRITGLPASIGTLIILISSTLFVLCYCFIPHFWYRACRGFGSPPLSVRAGLMCMGMTPFIFILSGKTNFISAMTGISYEKLNIYHQALGWGCLFLSLVHTIPFLVQPVREGGASNLSKTFKSDNLYQNGIPAQIVLGLLCLLSTRFSRKLCYEVWLHAHWILGIAYFALLTWHNVRGSTELDAEQYMWGTLGFWGFQMIYRALVKTSFFKPNQYVFKAKKGELSRLSNGNFQIIIKVETPYELNWKPGQHLFIRFVYGLSTLDNHPFSILSIPKQDSKSHIKLIVKPHNGLTKKIYNLIEDDDEKAGKQLNLYIDGPYGGINRDVLSFDKVLMVSSGSGVTVTWPFVEYIVDNLDKLTNMTQQFEFKWIIKNNESIEWIQEELINIQPKIVHNKQEVLENTDSSSSTDDLKSIEEKISISNLHHFITIHKGQKPSMSSYLQQVELLSKNCVIVSGTQSLQCDVGNAVSQLQRKVIKGEIDELYLHSENFGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYH6</accession>
    <name>A0A1E3NYH6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome biogenesis protein ERB1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Eukaryotic ribosome biogenesis protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ERB1</name>
        <name evidence="10" type="ORF">WICANDRAFT_64317</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat BOP1/ERB1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037380.1">
        <property type="nucleotide sequence ID" value="XM_019183710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYH6"/>
    <dbReference type="STRING" id="683960.A0A1E3NYH6"/>
    <dbReference type="GeneID" id="30200956"/>
    <dbReference type="OrthoDB" id="5571054at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000061">
        <property type="entry name" value="Ribosome biogenesis protein BOP1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03027">
        <property type="entry name" value="BOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028598">
        <property type="entry name" value="BOP1/Erb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012953">
        <property type="entry name" value="BOP1_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605:SF0">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1 BLOCK OF PROLIFERATION 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08145">
        <property type="entry name" value="BOP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01035">
        <property type="entry name" value="BOP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BOP1 N-terminal" evidence="9">
        <location>
            <begin position="165"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="429"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="771"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="23"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="797" mass="90632" checksum="0A1425FDD09C54D6" modified="2017-01-18" version="1">MAKKGSLKRAPVVEAPRKRKIEEESEESVEESEDEELNQDDEINVNGLIDDEASEDEEEYSEEEEEEDSEDDSDAEFNTMLGDEPENISDLESLEFEDEPKDNTSISDKLSNVHIRSLSDASDKQTTYIDGTPRVIKPEIEPVYESDDSDVENFNTIGNIPISAYEEMPHIGYDINGKRIMRPARGSALEQLLESIDLPEGWTGLLDSNTGGNLNLTKEEIELIRKLEINQSTDDSINPYEPTIEWFTSKQEIMPLTATPEPKRRFIPSKHEAKRVMKIVRAIREGRIVPNKPAVDEEEEHNYDLWGDGAQDNVDHIMTLRAPKMAPPTNEESYNPPEEYLLNDEEIAKFNEMEPSERERNFIPKKYGSLRKVPGYEENIRERFERSLDLYLAPRVRHNKLNIDPESLIPELPSPKDLRPFPIRCSTSYVGHKGKIRTLSIDPSGLWLATGSDDGTVKIWEVLTGREVYSIRVVGKDNDEDHVESVEWNPDQSTGILAISAGEHIYLVSPPIFGFDIENTGRLKLESGFGFNKFGNVKKNDLNVNEDSDLEEEAVSNKKEYARWIKPSEEMIAQGVVLTVECRKTVKSISWHRKGDYFVSVSPDSGNSSVLIHQISKHSSQSPFKKSKGIIMDVKFHPFKPQIFVCSQRYVRVYDLAQQVLIKKLLPGARWLSKIDIHPRGDHLLASSFDKRVLWHDLDLQTTPYKTLRYHEKAVRSIKYHKGGLPLFASASDDGNIHVFHGTVYDDLMTNPLLVPLKKLSGHKVINSLGVLDIVWHPKEAWLFSSGADGVARLWTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYM1</accession>
    <name>A0A1E3NYM1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_95938</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037508.1">
        <property type="nucleotide sequence ID" value="XM_019186505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYM1"/>
    <dbReference type="STRING" id="683960.A0A1E3NYM1"/>
    <dbReference type="GeneID" id="30203751"/>
    <dbReference type="OrthoDB" id="196165at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030950">
        <property type="term" value="P:establishment or maintenance of actin cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053039">
        <property type="entry name" value="Polarity_Bud-Selection_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47775">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47775:SF1">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="108"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="57"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="217"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="380"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="60"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="341"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="388"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ58301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="494" mass="56054" checksum="8E01F32A8C00A1CB" modified="2017-01-18" version="1">MSDTKLKELLKTMGSKLLDDDEDFKLTEPKRIVSTGSSSASEGSVVVHKKFDQTLQNIQDGDEEASEDDEGEQPLRIKTKNLQEEETDEDDDFNDYVIEPPLPPPKELHPGKLYALYEFSGNDPSHCELERDDAVFLLNDQDSYWWLVKKEFDGKIGFAPAECLETYHERLARLNCWKNEELERNSKETLEKFPNRLRSDSRVNKCVAFSNNVIITDEIEDEKSQSDEDSDDEEHEEDDEQESNYDDDEYNDQIPTEEDEVLTVSDNFPVTPLTIAKKNKPKPRAYSESDFDDESPLVAPTAFFNINNNTSGSIGTYSPSSSEFDSPGHTPPLTQFRKPVKEQLDEHSEKKGNIPQSKGTTDMFNSIRMLDEIINDELNTTKSLSDGNEGDDTITYDDDDDDKLSSPITLDDEKKNLSNMTNSTFTNSNTSLQLTSTTTSVDNDSISKDYSYNDLSTPKLESTSHPDIESIFQGSLSKMDELAEKLRLLNERFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYN6</accession>
    <name>A0A1E3NYN6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_64355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037422.1">
        <property type="nucleotide sequence ID" value="XM_019183716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYN6"/>
    <dbReference type="GeneID" id="30200962"/>
    <dbReference type="OrthoDB" id="10578417at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="185" mass="20459" checksum="DB5F3A27772FA19A" modified="2017-01-18" version="1">MDSLSSSPLTPATSIAPPATPPPRSSARANPSTPLINPPSSDSESEHESPLLNSLQLSPNEQSYLDQISSAPRTPVRPPTFTRSHKHTMSIPAYKFPSLRNFNVVNSSPSTPTGSRVDIFKDVKINELPQGGVSKLQGNRYSVYVQNEDFEIPERKKWFKHGKNNSFGSPGGWFLKNHKKSRSEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYU0</accession>
    <name>A0A1E3NYU0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">YL23</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_80356</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037411.1">
        <property type="nucleotide sequence ID" value="XM_019185052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYU0"/>
    <dbReference type="STRING" id="683960.A0A1E3NYU0"/>
    <dbReference type="GeneID" id="30202298"/>
    <dbReference type="OrthoDB" id="1478556at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.250:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_uL11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="10">
        <location>
            <begin position="13"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="9">
        <location>
            <begin position="74"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00064933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00080111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00082757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="165" mass="17815" checksum="9E6F782B3B7DB6C6" modified="2017-01-18" version="1">MPPKFDPNEVKFIYLRATGGEVGASAALAPKIGPLGLSPKKVGEDIAKATTKFKGIRVTVQLRIQNRQAVASVVPSASSLVITALKEPPRDRKKEKNVKHTGNIPLEEIIEVARQMREKSFGKTLASVTKEILGTAQSVGARVNFKNPHDIIEAINAGEIDIPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYV8</accession>
    <name>A0A1E3NYV8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_80543</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037608.1">
        <property type="nucleotide sequence ID" value="XM_019185083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYV8"/>
    <dbReference type="STRING" id="683960.A0A1E3NYV8"/>
    <dbReference type="GeneID" id="30202329"/>
    <dbReference type="OrthoDB" id="10049244at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071618">
        <property type="term" value="F:lysophosphatidylethanolamine acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007114">
        <property type="term" value="P:cell budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006642">
        <property type="term" value="P:triglyceride mobilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07229">
        <property type="entry name" value="Pat_TGL3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021771">
        <property type="entry name" value="Triacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF44">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11815">
        <property type="entry name" value="DUF3336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="6">
        <location>
            <begin position="192"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="538" mass="62079" checksum="8B21D3E0411260FB" modified="2017-01-18" version="1">MLVVPRTYLLSLLSLILNNIPPSLWVLIDFIFDISTFWLRIWRRKNPINVAKQNLLKAETYEEWQVGAYYIDRLTGSDLWRHNFTSTKYDYRLISERLKALQEARESNDIPQLLKLFRTGGFLRNFGGISNKELFNRAYAGTKTLIEDYISEILHCLHYLDEADIMEHEMSSNQLKLDFFHDARQAFGSTALILQGGSLFGLCHLGVVKALYFKGLLPRIISGSAVGAVVASLICSLTNDELADILLTITEHLGNIDRLNHDVDERYGSVIENVITKGYSQDILIFMKYVKDLIGDMTFEEAYLKTEKILNIIVHPTDKNIPSLLNYVTAPNTVIWSAVYASIGTGILSDNVMLYEKDLNNNIVPREYTDKEFTFLTPQKANERGNPESPYTRVTELFNVNNFIVSLARPYLAPLIVNDIKHKSGWKPKRVLQRLLGLELQHRVELMDRAGILFNFVKRMAVDEKTPKNSSDITIVPELRTLVKDFGRVFDVHRTMENIPYWILVGERSLWPVLPILWTRCAVEFTLDDLYNRRRRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYW5</accession>
    <name>A0A1E3NYW5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_85861</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036984.1">
        <property type="nucleotide sequence ID" value="XM_019185958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYW5"/>
    <dbReference type="STRING" id="683960.A0A1E3NYW5"/>
    <dbReference type="GeneID" id="30203204"/>
    <dbReference type="OrthoDB" id="2284405at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006525">
        <property type="term" value="P:arginine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900079">
        <property type="term" value="P:regulation of arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900081">
        <property type="term" value="P:regulation of arginine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00266">
        <property type="entry name" value="MADS_SRF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000002">
        <property type="entry name" value="Serum response factor b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033897">
        <property type="entry name" value="SRF-like_MADS-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0056">Arginine metabolism</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="8">
        <location>
            <begin position="44"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="125"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="135"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="189"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="226" mass="25225" checksum="FE8B7F1DA8740635" modified="2017-01-18" version="1">MSEITEPKLDDNQEDLVDNNQSIQNTTNPNEDLDDDDEKPSSARERRKIEIRFIQDKSRRHITFSKRKAGIMKKAYELSVLTGTQVLLLVVSETGLVYTFTTPKLQPLVTKPEGKNLIQACLNAPEESGEDEEASTPTNSTSQQSQNQQATQQQQAAQQQASQQQAQAQQSQPQQQQHPNQPPAGSGYPQQPGLQNLPPNSGYLNPEQAAVYQTYFGNINNQQGQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ09</accession>
    <name>A0A1E3NZ09_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_47209</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the importin alpha family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ57827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019182601.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3NZ09"/>
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    <dbReference type="OrthoDB" id="29145at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042564">
        <property type="term" value="C:NLS-dependent protein nuclear import complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097718">
        <property type="term" value="F:disordered domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031144">
        <property type="term" value="P:proteasome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.690:FF:000003">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000021">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.690">
        <property type="entry name" value="Importin-alpha, importin-beta-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032413">
        <property type="entry name" value="Arm_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002652">
        <property type="entry name" value="Importin-a_IBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036975">
        <property type="entry name" value="Importin-a_IBB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16186">
        <property type="entry name" value="Arm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01749">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51214">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="IBB" evidence="9">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="127"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="169"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="337"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="24"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="537" mass="59644" checksum="BAC58BE72E57383B" modified="2017-01-18" version="1">MDSDSTNKYIPDHRRTNFKNKGRFNPDELRRRRETQSVELRKAKRDETLAKRRNFNVSNANDSDDEDDAKKNDDDSSFLSQLQQELPRMMEQIQSDNFDSQLAATVKFRQILSRERNPPIDLVIQSGVVPRLVGFMSDQPEMLQLEAAWALTNIASGSSDQTRYVVEAGAVPSFVNLLYSNSLEVKEQAIWALGNVAGDSAQYRDYVLSCNAMEPVLSLFNTTKMSLVRTATWTLSNLCRGKNPQPDWNVVSQALPTLAKLIYSVDVETLIDACWAISYLSDGPTEAIQAVVDARIPKRLVELLGHETTLVQTPALRAVGNIVTGNDLQTQIVINAGVLNALLPLLSSAKDSIRKEAAWTISNITAGNSEQIQAVIDANLIPPLIKLLEQADYKTKKEACWAISNASSGGLTKPDIIRYLVSQGCIKPLCDLLSVADNKIIEVTLDALENILKMGELDKETRGLAENENAIYIEECGGMEKIYDCQNNENAKIYEKAYQIVDKYFAAEDELDDTGLAPEQAGNTFAFNTGNQANFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZL4</accession>
    <name>A0A1E3NZL4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Uracil catabolism protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_34821</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DUF1688"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF07958">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="48438" checksum="A1335325DBF149FC" modified="2017-01-18" version="1">MTKQENIKHLLTIQSVRERTQPIFKLALDNNLDYFDVDLKKIPDVIQFVTGVIVRDFKDLSTIPPHGRWGHLNAGGHDRVEQLISKWRASLFDDVEIGKKLVDLFVFSVLVDAGAGNSWSYTETATGESYNRSEGLAIASIDLFINGELSANTKDPYVVNGSKLVEFTKEDLLKGFQISESNPLSGEDGRLNLIQNLGKALVSNKEIFGEDGRPGNLIDYLKKKTYPDGKTIEFPIIWDALMEGFTPIWPKGRVEIDGISLGDAWPLSTTSKVGAKEFIDTIVPFHKLTQWLSYSLLVPLQKYGYEFEIKEQSLQTGLPEYRNGGLFVDFGVLDLKKEHLERGLELSKEINSKTPEIPSFKPDDGVIVEWRAITIGFLDYILPLVNKELDYELSLPQLIEAGSWKSGREIAAKLRPSTKGPPIDLFSDGTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZM0</accession>
    <name>A0A1E3NZM0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF866-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_34149</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0587 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037647.1">
        <property type="nucleotide sequence ID" value="XM_019182154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZM0"/>
    <dbReference type="STRING" id="683960.A0A1E3NZM0"/>
    <dbReference type="GeneID" id="30199400"/>
    <dbReference type="OrthoDB" id="10248838at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008584">
        <property type="entry name" value="CXXC_Zn-binding_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12857">
        <property type="entry name" value="CXXC MOTIF CONTAINING ZINC BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12857:SF0">
        <property type="entry name" value="CXXC MOTIF CONTAINING ZINC BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05907">
        <property type="entry name" value="CXXC_Zn-b_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141678">
        <property type="entry name" value="MAL13P1.257-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="18459" checksum="0774760C36ABF65D" modified="2017-01-18" version="1">MGYKLVLKATLEGLTVLRPKDTEQTPFEYTFEIECTSCREKHDKEITINRFEQHEISGSRGEANFVFRCKNCKRESSASITRTKNNYTIEDSGKAVSLLEIEARGIEFVRFVPDGLFEAKGAESTTKFEEIELNEDEFYDYDDNAGNEVSITEVSWDVVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZS2</accession>
    <name>A0A1E3NZS2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Aspartate aminotransferase</fullName>
            <ecNumber evidence="8">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_64323</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="8"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037389.1">
        <property type="nucleotide sequence ID" value="XM_019183711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZS2"/>
    <dbReference type="STRING" id="683960.A0A1E3NZS2"/>
    <dbReference type="GeneID" id="30200957"/>
    <dbReference type="OrthoDB" id="6752799at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006533">
        <property type="term" value="P:L-aspartate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000066">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000001">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF22">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="9">
        <location>
            <begin position="80"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="460" mass="51226" checksum="9453C118BB7FDA05" modified="2017-01-18" version="1">MLRHPLRTTPFRSISTKPSSWASLSSAQAKSSSQSTSASAANHQRLSKEDASLFWKGIPLAPADPILGITEEFNKDSNPKKINLSVGAYRDNHGQPYVLPSIKMAGKYLYEHESNKEYTGINGSSRYNNLVKKFIFGSSELGQSFIKNDQISISQSLSGTGALKIAGEFLSNWSPYQSRSIYLPDPTWANHHNIFKGCNLNPKLYSYFEPSTNSINIDNLLSDLSKAEPGSTVLLHACCHNPTGLDPSRDDWAKIIETISDSQLIPIIDMAYQGFQSGSLSKDLFLIEMILDQVNQSKLPTALICQSFAKNMGLYGERIGSFSIITPNAETTEKVDSQIKKSIRSIYSSPSIHGAKLVEIVLSNESIYKQWEKDVLQMSNRIKEMRVALYDLLKTKNPNSKWSHLINQNGMFCYTGLSKEQILQLRSEFSIYATLDGRFSISGINDGNVEYLADSIDKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0C9</accession>
    <name>A0A1E3P0C9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_19641</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037534.1">
        <property type="nucleotide sequence ID" value="XM_019180834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0C9"/>
    <dbReference type="STRING" id="683960.A0A1E3P0C9"/>
    <dbReference type="GeneID" id="30198080"/>
    <dbReference type="OrthoDB" id="4018368at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="114" mass="12347" checksum="BB4134548F65BEC1" modified="2017-01-18" version="1" fragment="single">ESENKDLDGKGLYSRHEGAAINYVFLADEAQTLNLNTTSGAIYLSAGNSDGQSFPQFLSVLENGFPGLQVSASGAPVTTWTFEDGYLKGNGSDAFFIAKDTKDPYNYSKDNYQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0D3</accession>
    <name>A0A1E3P0D3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_63423</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038123.1">
        <property type="nucleotide sequence ID" value="XM_019183547.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0D3"/>
    <dbReference type="GeneID" id="30200793"/>
    <dbReference type="OrthoDB" id="3979511at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05325">
        <property type="entry name" value="carb_red_sniffer_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000599">
        <property type="entry name" value="Uncharacterized oxidoreductase C663.06c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052184">
        <property type="entry name" value="SDR_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45458:SF3">
        <property type="entry name" value="CHAIN DEHYDROGENASE (ATSC), PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45458">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE SDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="256" mass="27953" checksum="11DE97330B6F5150" modified="2017-01-18" version="1">MSSSAKVYFITGVNRGIGFQIVKQLSEANPENIIIGTARDPTKATELQSLASSNPNIHILKLDVASEESIAALDAQLAPIAKDGIDTFISNAAISDSYHPVLETPKSNWYAHYNTNVIGPIEVSKVLYPYLLKKETRQLIYCSSLVGSLSGFFPISTAAYGQSKAALNHTVLTLSHELKDKGFTVVAVHPGNVSTDMGNYGMDTLSKNHPELGKKLVESYITPEASASYQIHDVFEKLTKESNGKFFNYDGSDYPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0D4</accession>
    <name>A0A1E3P0D4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_63416</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038116.1">
        <property type="nucleotide sequence ID" value="XM_019183544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0D4"/>
    <dbReference type="STRING" id="683960.A0A1E3P0D4"/>
    <dbReference type="GeneID" id="30200790"/>
    <dbReference type="OrthoDB" id="809632at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051397">
        <property type="entry name" value="Zn-ADH-like_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677:SF11">
        <property type="entry name" value="ZINC-CONTAINING ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="36334" checksum="CEF849FA44922AA4" modified="2017-01-18" version="1">MSTVKAVVIKSGDSKPRFIENFSLREPAESEVRVKVLGTSIHHLVRMRALGKHYSVSANDTSEKLIGIDGVGLIDDKQLVFFITLTPGTGSFAEYVNVDKRFIFPFPEEITKDDTDAIARVTTLSNPAMSSYLVFDSRLSQVIEPGFTVAINGVTGASGSLAAEIARKKYGASKVIGVGRSEDKLKALVEPKGLDAIIPLTDSDEEMTKKFSEFDVDVVLDYTWGKAAERTLLNLIKSRKDSTKLLSFVQIGQIGGTDFNIPPGILRGHNLHILGSGLGSFTTRDAGKAFPDITAQLAKGQFGTDEKIETAKASDIEQEWDLWQPSARKVFTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0E0</accession>
    <name>A0A1E3P0E0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 135 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_69322</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038126.1">
        <property type="nucleotide sequence ID" value="XM_019184161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0E0"/>
    <dbReference type="STRING" id="683960.A0A1E3P0E0"/>
    <dbReference type="GeneID" id="30201407"/>
    <dbReference type="OrthoDB" id="291792at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="56006" checksum="3E91A566341C9C27" modified="2017-01-18" version="1">MPEVNDKVTKSYKSLLFIGQFLLKVGDLESLSDSLPGLLSRVGVHDEDKRIKLIDSFRLIVDDFKKVQASNNGKVELAARTTRNFVRSSLAFFGFLTTVTSIKNLLINLSRKRLDSSISLDSFKNLGLVSASFGALLSGVPLTDAIVSRLLPFLSNTKHLSAFITSLAAFQLYPKDYSRDLLSIYLLVYGGESVQNYLIHQGPLKALKENCKLTDLVTKSWFLIPLTLSELYQTYVEHPLLTPTFVKKVFDSLFNDLNNPNPLWVEKGWPLVVKRVTILADNTLLQGEYIKRIKSLPSSINPINKLNGSANLKLFLRYIPSKFFKLMLFITPFYLLKDIITKKITLQDLKAKWKSYFKSLLQGTSKLSLFAILTTITAQFLTQQKLETRTISKYINPHRLIGFIAGLWGLLYRDSTSNALMSYILRTTLLSTWRKQTLKHKSLNHYNIELLLNALGISILVGFRENGLEDFITPKRFKNITEWISNGGQTTTQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0I8</accession>
    <name>A0A1E3P0I8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_63482</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038186.1">
        <property type="nucleotide sequence ID" value="XM_019183557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0I8"/>
    <dbReference type="STRING" id="683960.A0A1E3P0I8"/>
    <dbReference type="GeneID" id="30200803"/>
    <dbReference type="OrthoDB" id="4093878at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="237"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="336" mass="38238" checksum="6B66370903E3266C" modified="2017-01-18" version="1">MLSFDYAPASFFNSDCFMRSPMLDSNILLEHQNHYPLYHHHQQQQPRRIVRSKSYQPRRPTQEDYFASLLNQLIEHSGEEQKPLKPEVEAKVLNARDSFQIQVFKDNNNFNHYSIRYKLSGHDVIIYIESEVDDFAKGFRFAQDKIDINNVEWKVVRNVLVINVPKCKDEFILRPSVRFVKRVDPPACNNKPMVQQPKQAPVLISKPQRESTSTPPKEAPAVETRSSSPQEKLVTGASSPVTTSTVSSKPSPAPKSKVISIPIEFADSETEITPKSTRESSRRSSVTESVKSNTRTESEAELSDDDSAPSNKLPKLKRKVSIEEVEDESLHMADLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0J9</accession>
    <name>A0A1E3P0J9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_57356</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PGA52 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037659.1">
        <property type="nucleotide sequence ID" value="XM_019182799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0J9"/>
    <dbReference type="STRING" id="683960.A0A1E3P0J9"/>
    <dbReference type="GeneID" id="30200045"/>
    <dbReference type="OrthoDB" id="118256at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018805">
        <property type="entry name" value="YJL171C/Tos1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018807">
        <property type="entry name" value="YJL171C/Tos1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31737">
        <property type="entry name" value="PROTEIN TOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31737:SF2">
        <property type="entry name" value="PROTEIN TOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10287">
        <property type="entry name" value="YJL171C_Tos1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10290">
        <property type="entry name" value="YJL171C_Tos1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133615" description="glucan endo-1,3-beta-D-glucosidase" evidence="9">
        <location>
            <begin position="21"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall protein YJL171C/Tos1 N-terminal" evidence="11">
        <location>
            <begin position="35"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall protein YJL171C/Tos1 C-terminal" evidence="10">
        <location>
            <begin position="214"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="163"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="431"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="164"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ58452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="454" mass="47591" checksum="A9EF609AB100275B" modified="2017-01-18" version="1">MRFTNTILLSLLAAFTTVEAGCSFVGGNYYCDEVDAVVYSNVGYSGSYSDVTNMDEKSCECSQKSVSFEGSNSPLDEELSVHFRGPVRLLQFGVYYPGSGSNNQKRDVEEVEGCTTTKHVHHAHKREAATKVVEVTQTVFVDANGQLVTTTSSSSFEAIDKKVSTSSSNGQSSATDSTTTLSSTGAQSSSSTSSSSSSQSSSSSSSGSGSGSSSDWNRVAYYQPGSTQNCTFLNTKGGSGSGTWSSCFGNSLSYAASNGVDGASSANALDKVLLKSDEEYAIFSGSKCSGDSCGYYRSDIPAYHGFGGAEKIFVFEFEMPHDSSSSGASNPDMPAIWLLNAKVPRTLQYGNSKCSCWSTGCGELDLFEILSKGDERLISHLHSGQGSNGSQRGGGGSQDYFSRPTSGSFKGAAIFTSDGEIHVVKVDDGSDFSSGLSKSTVSSWLSQTGGDAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0V9</accession>
    <name>A0A1E3P0V9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_93508</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038051.1">
        <property type="nucleotide sequence ID" value="XM_019186416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0V9"/>
    <dbReference type="STRING" id="683960.A0A1E3P0V9"/>
    <dbReference type="GeneID" id="30203662"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16489">
        <property type="entry name" value="mRING-CH-C4HC2H_ZNRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051878">
        <property type="entry name" value="ZNRF_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46661">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZNRF1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46661:SF4">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="169"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ58844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="215" mass="24306" checksum="EB2D0C12330E8292" modified="2017-01-18" version="1">MTSYEPGTYVVSPQSQVFMQSPHVPHRTCDVCVEQMAMENSLRDSVITASSPMEVPPRNQVKDVSMQRTVGVNEPDDDIEQEREHCPICNTNLSGQTELQREEHINSCLINAEFSGSPEQFRSNRMLVYHIPFPDPKKVVVSCSDASTSTLHQQSQSNVDEKFPRDDECVICLEELKPGDKVGRLECLCVFHYKCIKGWFKRKGPGECPVHAVHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0W1</accession>
    <name>A0A1E3P0W1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_55261</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038170.1">
        <property type="nucleotide sequence ID" value="XM_019182756.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P0W1"/>
    <dbReference type="GeneID" id="30200002"/>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18020">
        <property type="entry name" value="DEXHc_ASCC3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000198">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000002">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000231">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000241">
        <property type="entry name" value="Antiviral helicase SLH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000102">
        <property type="entry name" value="RNA helicase, activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000024">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000004">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000001">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000062">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF13">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039073">
        <property type="entry name" value="BRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="278"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="501"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="1131"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="1336"/>
            <end position="1530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1926" mass="217899" checksum="57E0B923ED44B850" modified="2017-01-18" version="1">MATQYSVASTGSYMKAMQAMVDALEKLDEAAEKLATPKIDTEEHQIDLSYFDKFDDIYLAEEISDEISDLEDFDEANDSNAYDSFVKECERIADSREDISKLDLIDSIISTLSTESSPDSLQSLLFDILGFEEFDLISTIITDKDAILGDMSKESAQPKAQKEFFSERDIRKQVKRNQTLAKKQELAPAETVKKYPNVYKAFDGGNTMAFNGQKFTLPVGSTREMHENYEEIVIPTQASKRLKKAHERPVFISDLDTLCQGTFKGYTSLNRMQSLVYPVAYNTNENMLVCAPTGAGKTDVALLTILHTIGQFTTETEDTIDIDYSEFKIVYVAPLKALAAEIVEKFSQKLAWLGIKVRELTGDMQLTKAEILTTQVIVTTPEKWDVVTRKGNGDNELVSKVQLLIIDEVHLLHEDRGSVIETLVARTLRQVESTQSMIRVVGLSATLPNFIDVADFLGVNREIGMFFFDQSFRPVPLKQELIGVRGKAGTRQARENIDRVSYDKLIEELNNGAQVMVFVHSRKDTFKTARTFISMAQENNELGVFDCTDSESHDKFTREMGKNHNKDMRELFQYGFGVHHAGMLRTDRNLTEKMFASGAIKVLCCTATLAWGVNLPAAVVIVKGTQVYDSKKGGFADLGISDVIQIFGRAGRPQYEKYGTGILCTTSDKLDDYVRLLTSQHPIESKLSDKLVDNLNAEISLGTVTNVDEGVKWLGFTYMFVRMRQNPFAYGIDWKELAEDPQLVERRTQLIISAARRLHLLQMIIFDERSMHFVSKDLGRIASDFYLLNDSVEIFNQICSPNATEADILSMISMSSEFDSIKFREEEAVELTKLLEDKAKCQIGAELSTAPGKTNILLQAFISQASINDSALYSDSNYVAQNSIRICRALFLIGIHRRWGVFAKVLLSICKSIERRLWSFEHPLKQFDLPQQIIRNIEAKNPSTDDLRQMDSRELGDLVHNNKMGSTLYKLVGRLPHILIDGEIFPITRNVMRVHVTLEPDFAWDEKYHGNAQIFWVLVEESDKSQILHFEKFILNKRQLKNPHEMDFMIPLADPLPPQIVIRVISDSWIGSETTHSISFQHLIRPHNETLQTKLLKLQPLPVTALHNPKVEAIYSPKFRYFNAMQTMTFHTLYNSNSSAFVGSPTGSGKTVVAELAIWHAFDEFPGSKVVYIAPMKALVRERVDDWRERLTAKTGHKVVELTGDSIPAAKEVKEASIIITTPEKFDGISRNWQTRKFVQQVSLVIMDEIHLLASDRGPILEMIVSRMNYISSQTKNPIRLLGMSTAVSNAFDMAGWLGVKEGLFNFPQSVRPVPLEMYIDGFQDNLAFCPLMKTMNKPAFMAIKAHSPTKPVLIFVASRRQTRLTALDLIHLCGMEDNPRRFLKMDEFELQDVLADVKDETLRLALQFGIGLHHAGLVDSDRKISHKLFQANKIQILIATSTLAWGVNLPAHLVIIKGTQFFDAKIEAYRDMDLTDILQMMGRAGRPAYDTSGIAIVYTKESKKMFYKHFLNVGFPVESSLHKVLDNHIGAEIAAGTIKTRQDAMDFLTWTFLYRRAHNNPTYYDIQDISTAGVGQYLSNLIDSTIESLKESSCVIAGEKVLTPTPYLTISSYYYLSHLTIRNLVNQITSDASFRDCLRWLAEATEYNELPTRHGEELMNMEMSAQLRYPAEDLVDKPIWDPHVKTFLLLQAFMSRVDLPIADYSQDTVSVLDQALRILQAYIDVASELGYLNTVLMFIKIMQCVKQGCWFDDDPVSALPGIDLQSKNENGSKHLTTLAELGTFHGRKLDNFTKRLKVHAEFKKDLIYNVSHLPVGEFSILQDSPEELHVSLQHTNPPVNPDFKMLCAKFPKLQKESWFVILADRTNQELLLVKRAAPVIRKGYSKGIVSCDLTIPEDLQGLELDIIMVCDAFDITYSKPIKLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P104</accession>
    <name>A0A1E3P104_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U6 snRNA phosphodiesterase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">3'-5' RNA exonuclease USB1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_79545</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038215.1">
        <property type="nucleotide sequence ID" value="XM_019184910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P104"/>
    <dbReference type="STRING" id="683960.A0A1E3P104"/>
    <dbReference type="GeneID" id="30202156"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034477">
        <property type="term" value="P:U6 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027521">
        <property type="entry name" value="Usb1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13522">
        <property type="entry name" value="U6 SNRNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13522:SF3">
        <property type="entry name" value="U6 SNRNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09749">
        <property type="entry name" value="HVSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="37"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="298" mass="33888" checksum="204A1BE4C815EEDF" modified="2017-01-18" version="1">MEGIQDYSSDSSDEEEQGIDINTTNNKRSLPPLPQDLADKFTKRPKLPHPEFYDNPNYKNNKTTTMAPVGRLLRQFTYIDIRPSRQLSDQISDLNEILKMYLRMNGYGELLTRFERLHLSELSAPQALHISLTGETLFTKEGCKLYNDELSKKLKTIEMGNEDSNSITFDKIKAYPNFDSTGIFLAMSVSQKSSKTISKILQAVQELESKYVVKDSSRYPISPENLHCSIARVLTPVDYLKDPKTLQQINEVFKDLEVPESLEFKYSTVKVLEDSRSSLSFPIEAFTTKTSPSPSTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P152</accession>
    <name>A0A1E3P152_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mannosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_14421</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the third mannose to Man2-GlcN-acyl-PI during GPI precursor assembly.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 22 family. PIGB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037854.1">
        <property type="nucleotide sequence ID" value="XM_019180607.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P152"/>
    <dbReference type="STRING" id="683960.A0A1E3P152"/>
    <dbReference type="GeneID" id="30197853"/>
    <dbReference type="OrthoDB" id="416834at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF4">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ58647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="504" mass="59367" checksum="4B55D8E7D933D8D7" modified="2017-01-18" version="1" fragment="single">LIIIRIINALTIKTFFQADEYWQALEPAHQLVYGYGYLTWEWRTGLRSFIHPLLFSILFQIGELIGIGDLGVSILPKVFQAVVAAIGEYFTYRFIYQVTKNETIARWGLLISITSAFNWYIITRTFSNSLELTLTSIALAFWNWEKVVDWRSINISLLFSAISCAIRPTNGLIWVYLSIWFAFNKSLQQSTRLFLHAVIIGSIVIGINIIIDYFYYGELTVPIWNFIKFNVTTSLSKFYGVAPWHFHIFQSVPIILLTFLPIFIYGCFKYPNWQLKLLLAFVIGAFSCIDHKEFRFIYPVQPIMFLITSSGFYHLNLHHSKIVKYSAYIIIILNTLISLFFTQVHERGVIDVIEFLKYDPDVESIGFLTPCHSTPWQSYLHRPELQNSTWFLTCEPPLHLLNSTNSSKEVESYMDESDIFYEDPKAFLYQNFPPVFNKDLRSPGREYKYEWPTHLVVFQDLEPFISQYLKDSPYQECARFFNTYFHWDDRRRGDVIVYCKWPWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P156</accession>
    <name>A0A1E3P156_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">carnosine N-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_84776</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carnosine N-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.22" evidence="2"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038342.1">
        <property type="nucleotide sequence ID" value="XM_019185777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P156"/>
    <dbReference type="STRING" id="683960.A0A1E3P156"/>
    <dbReference type="GeneID" id="30203023"/>
    <dbReference type="OrthoDB" id="978at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030735">
        <property type="term" value="F:carnosine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035498">
        <property type="term" value="P:carnosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012901">
        <property type="entry name" value="CARME"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12303">
        <property type="entry name" value="CARNOSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12303:SF6">
        <property type="entry name" value="CARNOSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07942">
        <property type="entry name" value="CARME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01296">
        <property type="entry name" value="N2227"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="396" mass="46403" checksum="82548D4DE1D3E8C3" modified="2017-01-18" version="1">MNGDNEEYNALTRVLNAYYLYEDYARKELLSPRRLKWKSLTQEEQSLLPWFPEYLQVLEHCIKLNGEFTRNLATTVANDWGASSDPSQWAQCGYEDYDKVKYVFKQMLREWSEDGHEERDVSFNRIFQYLETKYPNVVERQHVKILIPGSGLGRLNFELVKRGFWCQGNEFSYHMLLASNFLLNHSYTRNHYSIFPLIHNFSNQLNRSLQTRPIFIPDLHNQTELINLQTEFPSIPVGELMSIASGSFTDLYGPDDLSISDHYSKDQNASEFRLQNANQFDIVITHFFMDTASNVIEYLKTLNHTIKQGGEWINFGPLLWHFEDAEDVVEIIKQDGTKVPTPVKGLELTNQDFLELAQNWFEFKHHEKQIEVGYSSDPKGLGGWKYKCDYWIATKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P195</accession>
    <name>A0A1E3P195_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_84868</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038463.1">
        <property type="nucleotide sequence ID" value="XM_019185798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P195"/>
    <dbReference type="STRING" id="683960.A0A1E3P195"/>
    <dbReference type="GeneID" id="30203044"/>
    <dbReference type="OrthoDB" id="6770063at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF48">
        <property type="entry name" value="MULTIDRUG TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G02700)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="346"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="426"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="453"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="521"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="121"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="557"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="559"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="582" mass="65074" checksum="57115E18FD7E0994" modified="2017-01-18" version="1">MSDHSVRDLSSIDSDSIHGFQPSINENEEHSNKTLERLVTNNKGVDDIITRLETRAGQLGPLEDGIDIHEVATHAAPDSTFHPEDKWHYPIDEETGMRIVQFIENDEKDPRNWSNLYKWFLTFFLGTVCFDVAFCSAVVTGDVDGPMKTFHVSEEVIILTVTLLVIGFGVGPLIFAPLSEEVGRRPVYVVTLGIAVIFIIPCAVAKNIGTLLVCRFLDGLFFSAPMCLIGGNLAEMWRTEERGVAMSIFSAAPFLGPVIGPLVGGFIGWRWIYWVLLIFSGVLYIAMVILVPETHHQTILKSRAKKLRKLTGDETYRAISEIKLRTFKEVANETLLRPLILLSEPIVFLITLYMSVIYGLLYMFFFAYPIVYMEGKHWSASKTGLMFIPIAIGVIVATVVSPFFNNDYNKRAQKYSNRGELPPAELRLIPMMIGCWFVPAGLFSFAWSSYPSVHWAGPCISGFACGLGFNLLYNPANNYIVDSYQHYAASGLAAKTFLRSMWGAAVPLFTIQMYHRLGYEWASSLLAFISLACCGIPYFFFFFGKRVRAKSKYAYSPESAKHEISGAKDAEKVGSSSLSDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P198</accession>
    <name>A0A1E3P198_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tubulin-folding cofactor D ARM repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_79238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037899.1">
        <property type="nucleotide sequence ID" value="XM_019184859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P198"/>
    <dbReference type="STRING" id="683960.A0A1E3P198"/>
    <dbReference type="GeneID" id="30202105"/>
    <dbReference type="OrthoDB" id="10253476at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048487">
        <property type="term" value="F:beta-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058033">
        <property type="entry name" value="ARM_TBCD_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033162">
        <property type="entry name" value="TBCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12658">
        <property type="entry name" value="BETA-TUBULIN COFACTOR D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12658:SF0">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23579">
        <property type="entry name" value="ARM_TBCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25767">
        <property type="entry name" value="ARM_TBCD_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin-folding cofactor D ARM repeats" evidence="1">
        <location>
            <begin position="167"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="863" mass="99299" checksum="9BEECA8CC1EDE43A" modified="2017-01-18" version="1">MLNFMKTDIELIPTILSKIDQSQDIDWHEKYLLLCWLSVLCLAPFKLDSLRKDAKQRIFTIGVQYLKTSGPLQPLGARLLASLIMRSDCAAEFDKFMELLMEEYMDASEVAQQGYLETLNIALQKDSNMIFQPRLEQILSFTKSVEKSECHVDLVSKIYSKLVKYLIDMDDYDQIEEIITYFLNNFDNRSTDTRFLLAKKFVKLVSSLDSCFGIEIIIDIIHSTKELLLESFETINSDKLHTHLLTLAEFLRLSLVDKAGYKDIVEILDKTLFFQQSRITFIAGSNIRDASNFISWSLFKYNKDIDVSTAFSVFKKLLLVTCFDKEIMIRRSSTAALQELIGRYGNKIWEEIYPSEENSAKNIRLIEILDYIDLGSIEKSYFDIPSKVISLFPGLEQNFISFLSQNVYNLDYDVVKLSSRALKDLLSGQPQDVVHSIISSHFDKSSQKFNAFIVLSEILPLSQNKQYPQLKSTFQEIKLNHHKDSVSMITSYLSLLNTLLEIGYELDDWLLENLFDAIRVDNEEIKEVLIKIVSKITLSGKYWDKWIYYMRNNNLNTSSSVGYFPDFGSKVSDVVHLLSIDRVDADTKASIISSISIYLYHNQLDKEILGKLVEQLDDYTISEQGDVGNKIRSQTIKLIEHNINVFKDSSLSELVIPKLLRLSCEPIDKTRSDSFHLLMKFYGIPGHTFHSLDGYFTELLNIYKKLFMGDKETSKEFWKGFIFTAGAIKSTDSLIIASLKCVLRFYETLSSEAQKEILLQLASVIKIEPQALKGQNSKAQRLNKTILVGLQFWSRILDSNLLIPKEFPVKGLYVRIYNLHLNTKNINRLTSSIKIFGYLAHIPGLKEPLERLCWLCSKHPIAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1B0</accession>
    <name>A0A1E3P1B0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_79575</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ubiquitin conjugation factor E4 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038247.1">
        <property type="nucleotide sequence ID" value="XM_019184915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1B0"/>
    <dbReference type="STRING" id="683960.A0A1E3P1B0"/>
    <dbReference type="GeneID" id="30202161"/>
    <dbReference type="OrthoDB" id="20295at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034450">
        <property type="term" value="F:ubiquitin-ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071361">
        <property type="term" value="P:cellular response to ethanol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16657">
        <property type="entry name" value="RING-Ubox_UBE4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000055">
        <property type="entry name" value="Ubiquitin conjugation factor e4 a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019474">
        <property type="entry name" value="Ub_conjug_fac_E4_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045132">
        <property type="entry name" value="UBE4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931:SF2">
        <property type="entry name" value="UBIQUITIN CONJUGATION FACTOR E4 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931">
        <property type="entry name" value="UBIQUITINATION FACTOR E4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10408">
        <property type="entry name" value="Ufd2P_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="12">
        <location>
            <begin position="965"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="21"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1048" mass="119190" checksum="C96299DE2C44FA54" modified="2017-01-18" version="1">MSAEEIRAKRLAKLNNGSQPSSSTSLNSQNTTNSQTATITKKTTTSAVSSSTEQSTVSTPVLDNADAKRQKQSTSASTQENITSWTNKIFEIVLNVTLDHEKSGSAIFLENTFTELVEENQDPVFNKDLADRAIIEVLSEIGVDNPFSYLKNAWNKTFQERRIILQKDTLRDAKYEILNEIERLTSSYGLVSFQIPDLFINGFNVNSTLLDIISNENSYSDFLLQIIKRSDEEGTLLDFLNIFIPNLSKLILGLDLNNPKYPLILNIFQLFLSQKAVGAVFTQIEGFELNPDLEPSKFEVSSILGPIFKLSPLQRTVARNNFDRGNEKSQLQINKIGESLQAEHKVLLDRLFSITSKIIRGSDQSRHDLMKYFGTIINKNHLRRGDHSDANKLSSNAFITNISLILIRLSQPFLDSTLTKIDRIDPNYYSKTPLVDISEETRVNSTTAEATEYFNANTEIDKGLKTNFISDCYYLTLAYLHYGIGGTYLSETKMKSTIKRLEQSIARLRQQANTPNPMQGFAKVQLQRMEASLAALKSEKDALVSFFVHRDLQVEIFDFVSGASAFLTRMVDPKHTYPHGGLLKLPLVPDVIGFENVDSTDYFKEKAPSPFKYYPEYVVEGIINYCHYISKYSNNPMLRNPRLQTFVEFAVTFLRCPELIGNPHLKGRLVEVLFIGTLPTQDQRPGFMADIIDTDKLVSDNLLYALLDFYVIVEKTGSSSQFYDKFNSRYHISSILEQIWKNPLYQNQLKWQSENNEEFFIRFIARMLNDLTFLLDESLRQLAEVHLVQTELDKRERGITTLEGTVEELQSRFNSASGQAKSFCGLANKSIDLFGLFTQEVPRAFTKSEIVGRLASMLDYNLDALVGPRCVNLKVKEPEKYQFSPRQLLVNISKVYNNLSNEEEFINAVSQDSRSFKRSTFKRAEAILGNRGFVANDFLKTFIEFADKAEAKRLEEEEEELKLGEVPDEFLDPLMFTLMKDPVTLPGSKVNIDRSTIKAHLLSDSTDPFNRMPLKLEDVIPNEELKQQILDFKLKSKQDKGDVDMTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1E1</accession>
    <name>A0A1E3P1E1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Proteasome component ECM29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_79817</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038506.1">
        <property type="nucleotide sequence ID" value="XM_019184954.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P1E1"/>
    <dbReference type="GeneID" id="30202200"/>
    <dbReference type="OrthoDB" id="16066at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042030">
        <property type="term" value="F:ATPase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024372">
        <property type="entry name" value="Ecm29_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055443">
        <property type="entry name" value="HEAT_ECM29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346:SF19">
        <property type="entry name" value="PROTEASOME ADAPTER AND SCAFFOLD PROTEIN ECM29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR GCN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23731">
        <property type="entry name" value="ARM_ECM29_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13001">
        <property type="entry name" value="ECM29_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24492">
        <property type="entry name" value="HEAT_ECM29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Proteasome component Ecm29 N-terminal" evidence="6">
        <location>
            <begin position="9"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome adapter and scaffold protein ECM29 HEAT-repeat" evidence="7">
        <location>
            <begin position="1293"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1668"/>
            <end position="1690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1841" mass="203826" checksum="4F46ECC6A0931C1C" modified="2017-01-18" version="1">MADQELSLINKVELRIALADSDQKFQSALDLYLCPLLMKLSSIHGAVRQEILKFIQHLIPRINSYREIKLPTLKLIQQAKNQTSNADASTVQLYSLLFASKGIDRLSVEEKRELLPEVIHGVHNFGDNGVAARLFHIFCKLLQGWKTPDRGTDDFKQLRQDLAIDTNDEKFLVDKIEKLFLLAPVINDDGVIPKGYTCPGLSSKDTAFLTYDAGVSYKNDDLSNAKKNIVSFIPLFHDENLVTLLLIGVCDSNDSVSNASASLYRKINIPYEDGKLIDHLISLFIGDKEIPRSPVKSPIQERFLSVMIDSKIATKSKHIPIITSLGLNASRYPKLKAATIQFIQAVAKNGGDAILAGGESAEYSVNVAAQLRNNLHAEGWPKLETTQGSNYANDIKHRQLQYEALGDILKKDFNLVKDISYIEFLLDSLIGDLPVLRTTIQEALSSLVVHLPNLSLESKNKLKKILIPILEQDNFSDEGMDAINAVRFVAIKYINAAFPFEDHEARYLNVLGTSAKNRSDVIEEAQKGLHPYWFNILQSSNTAEFKSTNELMGVGNKVKFPRFCDFVNKLVTEVDGARNSDSSALRHAMIPAVEFALHVLVTQAIGNKSTVVVQDQEWITRLEKGLEHDETVISSVKKELQNVEGLSNFLSILLDEFAVKDNYGKDIAVSDISSPIFGKLFLKLISLSPQTVVEKLSPQVPRLIAIVENAKVTREDTIQQAANSIGIIASHPINSNEDNLQLLQNIVAFLQGADTKQLNNHGRLLTLGFLTSRLFLRGRDILSNDDVQKVYGIIGESVSSTDSRLSGAAITATSQLAQFGGFGPDLTNPDVDVIKKQILESLKAKVKKLGEKSMIAWSLLSLSEVESDSKDFTIFEEVIYDTHTTKQVESLFTSGEAFSIIAAGWDSKFLQKQVDIQSDDLKFRAAPRKTRLDFILSKILQASSSTKPSLRKAGCIWLLSIVQYCGHLPIIEEKSGSIHVTFMKFLADRDELVQEAASRGLSMIYELGNADLKETLVKSLLRSFTESTSSAKLGAGTVSEDTELFEPGVLKTDDGSVSTYKDILNLASEVGDPSLVYKFMSLAKSSALWSSRKGIAFGLGSIMSKSSLDKLLFENSSLSNRLIPKLYRYRFDPNPSVARSMNDIWTTIVQDSSKTIDEYHEGILKELLTGMGNKEWRVREASTVALTDLLQALNKDKYQDRMEEIWTMGFRAIDDIKDSVRKAGGGLTRSLSQMLVNSIKVESGSSETNAKEVLSKLLPFLLGTKGIQSDAEDVRDFSLETILKLVKRGGKAIKPFIAELIDQFVLLMSTLEPQIINYLTLNADKYNLKHDDIDAKRLQSIGSSPMMNALERLIDLIDDDIIGDVVVRIQSTVKKSVGLPSKAAASRLFVTLVVRHLQLLKPYGEVLLNTCISQLNDRNTTVSSSFATAAGYVCRVCSVDVVAKYGEKIQASYFESEDEKSKIVAGVASEAVSKYSGDRFASVASAFLPVAFVAKNDANKEVAKNFESEWTENTSGNGAVKLYIHEICALVKQYITSPQFSVRQTSAKSIAEACNAIDGATGIGSSADELFEVLMNACQGRSWNGKEDVLQALVSLSSKSKPFVDSHPELLAKINKIVITEAKRRNKDYQKHAIVSLGEFASIYPSEELHDQVLEIFEPLLSDEYYESEDEDEDMEKDEHKTVNAKSSQKNLTREAQRMKALSSLVKSFQIYPDKTYHPELLEFTLKALEGTINSNVIIPTWRSQIAVDEGLSKIAEVLKGASNIDSNNVDLLIKTWEIIFKNNTKTEDVENVKIHTVRAGKALIGLKNENIKYKVVDALKALDAIEPSSVIKVEIGNALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1L2</accession>
    <name>A0A1E3P1L2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein arginine methyltransferase NDUFAF7</fullName>
            <ecNumber evidence="7">2.1.1.320</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_93383</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Arginine methyltransferase involved in the assembly or stability of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48108"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:88221"/>
            <dbReference type="EC" id="2.1.1.320"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NDUFAF7 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.320" evidence="7"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038009.1">
        <property type="nucleotide sequence ID" value="XM_019186415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1L2"/>
    <dbReference type="STRING" id="683960.A0A1E3P1L2"/>
    <dbReference type="GeneID" id="30203661"/>
    <dbReference type="OrthoDB" id="5595109at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035243">
        <property type="term" value="F:protein-arginine omega-N symmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003788">
        <property type="entry name" value="NDUFAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038375">
        <property type="entry name" value="NDUFAF7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049:SF7">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02636">
        <property type="entry name" value="Methyltransf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="254"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="260"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="280" mass="32391" checksum="23DB38B8D2D63949" modified="2017-01-18" version="1" fragment="single">MLLRSRNPALLRQFSTSAVRCQVIPKAYKQSFPTRYDTARKKIELNNLSQIFTELIKTTGPIPLSAYMRLCLTHPDFGYYTTKDPLGPKGDFITSPEISQMFGEMIGIWLFTVWISQKKPKNINLIEFGPGKGTLMFDVIKSFSRLLQTLKASDLTLNIILIEASDVLKKAQWTLLSDSTLDVNKEFWTTDHKWNGKITWVDTEKDVWEITNDKDANYILAHEFYDALPINQFKKTEEGWREYLVDQHPETNDFHLTVSPKERPSSKIPETNKRYSNVTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1M1</accession>
    <name>A0A1E3P1M1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">VPS10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_20174</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038558.1">
        <property type="nucleotide sequence ID" value="XM_019180858.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P1M1"/>
    <dbReference type="GeneID" id="30198104"/>
    <dbReference type="OrthoDB" id="443634at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15482">
        <property type="entry name" value="Sialidase_non-viral"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.270:FF:000005">
        <property type="entry name" value="Sortilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.80">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031777">
        <property type="entry name" value="Sortilin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031778">
        <property type="entry name" value="Sortilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006581">
        <property type="entry name" value="VPS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050310">
        <property type="entry name" value="VPS10-sortilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106">
        <property type="entry name" value="SORTILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106:SF27">
        <property type="entry name" value="SORTILIN-RELATED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15902">
        <property type="entry name" value="Sortilin-Vps10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15901">
        <property type="entry name" value="Sortilin_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00602">
        <property type="entry name" value="VPS10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110296">
        <property type="entry name" value="Oligoxyloglucan reducing end-specific cellobiohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1316"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="domain" description="VPS10" evidence="8">
        <location>
            <begin position="1"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="VPS10" evidence="8">
        <location>
            <begin position="672"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1351" mass="150072" checksum="45D2EC3A1B4441C0" modified="2017-01-18" version="1" fragment="single">NVLYLDTGSNDLFFSKDGGAHFTQVDTEGIKIKYLVPHAFKPSHAYAISDDLTHFKTVSSGLVWKKFTSPESPALESIPSLTSKHYPISFHGQRSEFGIFAGRCSSDASSADVPCERQYYYTKDNFDSLEKLTKAHACIFADSAPNIDANQGDELVTCIVDGGEGTSLTLARSTDWFGTKSFATYKGGLITDVSKIGSSSHYLVAIGSHLSDRTTSNVYVSYNGYDWDKVEFPTGTELSSGFTIVETTDNSLFVDVSYPNTKSVGQLFTSIGLNGSYFTKSLDYTHRDEFGNVDYEVVESIDGIVLANVVENGKKVLEGETSKVLKSKISFDQGLTWKHLQSDGQPLHLHSLVNRASKNAEAPGKYFSSPVPGILIGVGNTGSSLNAYESCDTYMSRDAGLTWTQILNGPHLFEFGDQGTIIIAIEDDADVNSLKFTTNQGKTWQQVSLDSTIRAKLLTTTPDSTTFKFLLGGVSSNGQYKTYGLDFDGIYENKCGDSDYEDWFVKGPEFNQPLCFMGHKQKFKRRIRDANCYVGNEYREPVAVEDSCPCTESDYVCSYNFKRDSNGKCVPAVQGLTPENVKANSCVDNAQTYFEPVPYTLRPGSKCSLTGGLNLAVTVEKKCPNFVKEKNKDDHSEGKNDGRVKTSSFVFDGKITKYAYLDRVKGSNLKDETIVLLTAHNKAYVTHDQGSSWEQIAPDEEILDLYVNPYNTNHIYLLSTNQKIIYSTDRADNWKFFRTPATFIPGVQPLQFHPKHSNWLIYIGQLGCDNISHQGSCRTVTYYSKSYGKRFAKLHDHVKTCQYIGHLLEPTDAQLIICEREDSKSSGLNSELLVTEDFFQNTIVPFDDVIGFARADDYLVAATAEGDGSLRAHVSVDGKHWTDAHFPNNMKVSKDQAYTILSAASHSIFMHVTTNTRAGTEFGHILKSNSNGTSYVVTASNVNRNSGGFVDFEQLADLEGIAIINTVSNADDAKRGSRKALKSMITHNDGADWDYLQPPSVDSNGKKYGCTGRSINECSLHLHGFTERADPRDTFSSGSAIGMMIGVGNVGDKLGAYLDGSTFLTRDGGVTWKEVKKGVYQWEYGDQGSIIVLVNGQDSTNTISYSLDEGKSWSDYQFTDDLVKVNDISTVPSDDSKKFLLFTKLPSNRGDKSTVYQVDFSQLLKRKCNLDLKNPDTDDFDLWSPKHPHQPDNCLFGHEAQYYRKIAGKDCYIGEKLSEPFQVLRNCPCNREDFECDWNYERDSSGQCKLLAGYSPPDHSEVCRKQGIEEYWLPTGYRRIPLTTCVGGQEFDKVEVLPCPGMEEEFREHHGGLKGLSLFLVIILPILAAASVVYFIYHRYVGIYGQIKLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1Q2</accession>
    <name>A0A1E3P1Q2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_63342</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038044.1">
        <property type="nucleotide sequence ID" value="XM_019183528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1Q2"/>
    <dbReference type="GeneID" id="30200774"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="210"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="675"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="725"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="481"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="276"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="675"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="685"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="728"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="759"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="809"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="825" mass="93209" checksum="52A36B965D2404C7" modified="2017-01-18" version="1">MNQETVELLQGARNGGGDDGSMKWVDEPIIPKKPYLEDIQTADDTLKDDLIKAMLDIRALASSDPQMSRSTVKILLDFVEWTVTVEKRNALVFTEAMAVKYILYLIQENIFYSVDREDKSLIVGFETLKEIATSIVSSSLSLKKIDFRNTTIFTKLQLLNVDLQLLRDGKFENKTNVLARVKRILLTEPADFDPNDTATQPVNHLPVEGMEDEHFDSSPPIDSSVEGDRDESNHSKDLSNVNVLTQSTQINTINKSLDQSRVDVHATSSSNGDSQQSKDSHNGEHSRRDSLNSKSSRIKKYAKKIQDAQLRNELLHLAEELQAEDNSKTSFVDAYNQTETAFKTILQNLDAFKSVQSNLPSIQTFVEAIGLSNDTDISIRSTESSRIVSNESQEPVQPLTQQHIDQIRKDIGAEALERKESTKVISNYLDRRINKLSIETKTLRENLKAFNRSEVEVLNIKELLNKLKDSSVSKLDHQAIVRNLESKINNIEDVKHQMEAGLLSVTSIGDQTSTTVKRMEALERENRKLSNQMISINNYFLKVYDAQKSNIEMIQNFHKMLDAIESRSSAGSIKLEGFSADLQKIQGDIRKMNDFQGSTSKTLKTLAKQNSSGVFDFAAYKQIADKNIEDVKQQVQIIDETLQKKIDYDNTYINEKFDKVGIEIEKIIANQTKLMKESKPENSSRLHQLNSSPPPMNRNQLIRQGETSKKDLSSSPELLSEKVFHQTSVPRPSLSQSQSQPQPQPQPQQRRAPVPLSSKGPQNTKKRNMQISLSNSISSKITGLLQRNKKDKEPKTPPRKRSRSPSTREGSEGLKHIKLFRAIDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P208</accession>
    <name>A0A1E3P208_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_22056</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038575.1">
        <property type="nucleotide sequence ID" value="XM_019180926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P208"/>
    <dbReference type="STRING" id="683960.A0A1E3P208"/>
    <dbReference type="GeneID" id="30198172"/>
    <dbReference type="OrthoDB" id="442970at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF4">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE DOMAIN AND WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="5">
        <location>
            <begin position="3"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="176" mass="19586" checksum="FA36378802E4C834" modified="2017-01-18" version="1" fragment="single">RVFFAVEIDGKTVNVVIQLLIERAAKESIKNFHKLLTSNNGSSYRSTKLSRIVSPEFIIQGGAIDGFNGDLLVKNEVTPETTLLDQRGLVAIATEVENHEPSSEFFITLEDLSEFKALKGRFLVIGKIVNGLDQLIELTKDVAVDENDRPIHDIYITRTGELVKKKKTSAQNDTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P218</accession>
    <name>A0A1E3P218_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_84953</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038585.1">
        <property type="nucleotide sequence ID" value="XM_019185814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P218"/>
    <dbReference type="STRING" id="683960.A0A1E3P218"/>
    <dbReference type="GeneID" id="30203060"/>
    <dbReference type="OrthoDB" id="545910at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14279">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032854">
        <property type="entry name" value="ALKBH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31212">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31212:SF4">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="1">
        <location>
            <begin position="192"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="389" mass="45118" checksum="5795ADB35A992DE1" modified="2017-01-18" version="1">MTDAKLSHLKLSFPEADESSLLELLISCDGSLPTAESILFESFPNAKRRKINNHSKQSNSGVAKSSNTTNVNKPVYLYSKEDIETTVPYSTIHYDFLPKELADSLLETLIKDKKGFQPQEFYLFGNKCVSNHMSKVYGSSTSDELYYNGKKYKSRDRYSDSMKMTQLLIEDQVNKELKERPSLPFQSDIPWRGDLAVSNRFNSKSNNLDWHSDRMTYIGPHCVIASFTIGATRDFRIRKQYNDPNSKFNTMYAIPLPHNTLFIMHAGFQEEYKHCIPATTEFTPHEISGTIRMNLTYRHYLPKFQENLPMCSRCGNQMDLRRSFKDPKTRGRYIWVCTGGYLGKECNGFHYADFTKKCLYTDDVEECSRWISREDKEALQCFRESLMNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2L7</accession>
    <name>A0A1E3P2L7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial distribution and morphology protein 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Mitochondrial inheritance component MDM10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MDM10</name>
        <name evidence="7" type="ORF">WICANDRAFT_29758</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the ERMES/MDM complex, which serves as a molecular tether to connect the endoplasmic reticulum and mitochondria. Components of this complex are involved in the control of mitochondrial shape and protein biogenesis and may function in phospholipid exchange. MDM10 is involved in the late assembly steps of the general translocase of the mitochondrial outer membrane (TOM complex). Functions in the TOM40-specific route of the assembly of outer membrane beta-barrel proteins, including the association of TOM40 with the receptor TOM22 and small TOM proteins. Can associate with the SAM(core) complex as well as the MDM12-MMM1 complex, both involved in late steps of the major beta-barrel assembly pathway, that is responsible for biogenesis of all outer membrane beta-barrel proteins. May act as a switch that shuttles between both complexes and channels precursor proteins into the TOM40-specific pathway. Plays a role in mitochondrial morphology and in the inheritance of mitochondria.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the ER-mitochondria encounter structure (ERMES) or MDM complex, composed of MMM1, MDM10, MDM12 and MDM34. Associates with the mitochondrial outer membrane sorting assembly machinery SAM(core) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Mitochondrion outer membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="6">The ERMES/MDM complex localizes to a few discrete foci (around 10 per single cell), that represent mitochondria-endoplasmic reticulum junctions. These foci are often found next to mtDNA nucleoids.</text>
    </comment>
    <comment type="domain">
        <text evidence="6">Lacks alpha-helical transmembrane segments, suggesting that it resides in the membrane via beta-sheet conformations similar to those predicted for other outer membrane proteins and porin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MDM10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038933.1">
        <property type="nucleotide sequence ID" value="XM_019181645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2L7"/>
    <dbReference type="STRING" id="683960.A0A1E3P2L7"/>
    <dbReference type="GeneID" id="30198891"/>
    <dbReference type="OrthoDB" id="2103793at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001401">
        <property type="term" value="C:SAM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051654">
        <property type="term" value="P:establishment of mitochondrion localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070096">
        <property type="term" value="P:mitochondrial outer membrane translocase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03102">
        <property type="entry name" value="Mdm10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027539">
        <property type="entry name" value="Mdm10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28035">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28035:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12519">
        <property type="entry name" value="MDM10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1 6" id="KW-1134">Transmembrane beta strand</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="396" mass="44604" checksum="5EFB865D871E095A" modified="2017-01-18" version="1">MYEYMDYILKSFYKSTGWNEDNSYENIIATSQALIDFPIESDLKFSVSSKSSENTATSLSLTNSGAINGSIAYLYTLTPLKHVLGTSEISLQDAIQGYRVIRPPLYKSYNYKKDKPIFGAHSLYYGRMYFPGSALEAMVVKRISESSQILVKCVSHEKLKNNGTMTFYFQKDTGRLSREYIFSTNEALLGFRCLYNFRQGAERLNTALYNNSSLSIGAEIWYGAMNMSPGLSTALRYSTQSTYTGKPLTITLACNPILGHLSSTYSVRTSASSTFSSRYDFNIYSYDSDLGFGCELWRTSGNNSPVLPLRRIDPKLAPYSKHTSQDQTVIEAFESLVKETDFTSVIKIGTTMNDRDVKLKWEGKFKDFLISTGVDIQINSQVPDVKKWGLQVQYAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2W4</accession>
    <name>A0A1E3P2W4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_68251</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038865.1">
        <property type="nucleotide sequence ID" value="XM_019184111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2W4"/>
    <dbReference type="STRING" id="683960.A0A1E3P2W4"/>
    <dbReference type="GeneID" id="30201357"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF11">
        <property type="entry name" value="AMIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="3">
        <location>
            <begin position="71"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="869" mass="96911" checksum="7EE70516CDE636E2" modified="2017-01-18" version="1">MTWEEIAKAKREAVFNKIPKEWIIEVPSIEKEPNAVKYLDKVLPAQETAIVNHSMIELIDKIGKGELTSTEVANAYCHRAALAHQVFNCCSEIFFERAIERAKELDAYYKEHGETIGPLHGIPISLKDQVNLEGLDSSLGFISKVNKPKTKEDESIIAKFLYDSGAIFYVKTTVPMAMMAGDTFSNIYGQTVNSLNRNLSAGGSSGGESSLIAAKGGIIGLSTDLGGSIRIPAAFHGLFAIRPTSYRLPYAKVDNAMAFQPICPSVIGPSARFLKDLEYLCKVIIDCKPWLHDPKTPPIPWRPFEVPSKLCFGIYKNSGLTHPHPPIARAIELTRQKLIEMGHEVIEWEPPIPHEFIRDNLNSIFTSEGFNEIKEECAKSGEPVIGTLVAGETANGELLVSEHWKQVQVKYETQMKYDEYWRSTIKKTSTGRPVDAWISPVWESTSHLVGIGQPDCTGYTYPVNYLDLSSVIVPITSADKSIDTIYEDFKPFNEGDKRMNDTYDPEFFDGMPACVQVITSRYEEEKSKFLSQPEPEPIIEKFIEENGGLENITGQHSSKLLNLNPHDVLKDVNIFIGETKESLYDQWIVLYDKHAPVLLTDKVDSALSFIRSDKVWPYYEKYQKPIDNLLIGLAILILLRVVKVLARLVKPAKTIREIDEVTKVVLTEKDFETIPICVGKGDAEELLLIQSYADILKSSNDEEVLKKYGYDKDFSDTNSKSDLSILNDFSPSRIDSPNDFTASDVSAIFQTNQDIGLDAPVEIELRKGTESPSLEDSFERAENILDNKLESLNSSNMVVTPSSGGNSIDQYSTSPSKLIYKKTSISILTNNTFPTTASVADTTVGDITEETVYSEPFAGDENTSFKDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2Z4</accession>
    <name>A0A1E3P2Z4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TRUD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_78327</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038895.1">
        <property type="nucleotide sequence ID" value="XM_019184703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2Z4"/>
    <dbReference type="STRING" id="683960.A0A1E3P2Z4"/>
    <dbReference type="GeneID" id="30201949"/>
    <dbReference type="OrthoDB" id="447290at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02576">
        <property type="entry name" value="PseudoU_synth_ScPUS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.20:FF:000011">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="entry name" value="TruD, catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020119">
        <property type="entry name" value="PsdUridine_synth_TruD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00094">
        <property type="entry name" value="tRNA_TruD_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326:SF21">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE PUS7L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037016">
        <property type="entry name" value="Pseudouridin_synth_euk_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01268">
        <property type="entry name" value="UPF0024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TRUD" evidence="5">
        <location>
            <begin position="337"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="85"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="667" mass="76546" checksum="FAF46386241ADB32" modified="2017-01-18" version="1">MSKAKRPVESDSPIENVKKQKVESTPESAPSKGIVETDVGITSYISQDLAGFTGTLKQRYTDFLVNEIDLQDNVVHFTDEGIPDKKQRRRERRENERDVQKEEEPKEEPKEFQLDAAHRIKLLENFGEEELSKIEEVLKNGSKFESSKVFNEKKTRGEIHQLFREAFQSKIETKTTDKNTFQFSLATRNSRAFKPKQQVDTRDENGVENYGLGPLKDFLHFKLYKENKDTMEAANLLSKFLRLQPKQIRYAGTKDRRGVTVQKLSISKIKVERVNSLNRTLRGLKLGSFSYENEGLSLGDLHGNEFLITLRDVQSLDPSQPIEEVISKSLESLKTHGFINYYGMQRFGTFSVSTHTIGKEILLGNWKAAAELILSDQEFVLPDSKEARKIWSESKDVSKALELMPRKCVAEYTILKYLSTEKKEDTGDYKDHSYFQAIMKIPRNLRIMYGHAYQSYIWNSIASLRVDKYGLKVVAGDLIVEEEAEKPVNTDESAETEIFEEDVRTDKYQRARPVTEEEAASGKFSIYDIVIPTPGFDINYPEIPDIKAGYKELMEKDGLNPDDMVRKVKEFSFAGSYRSLIGKAKNLDYQIRHYEEPAQQLVYTDLELLHKKQKGEEVEQVLPDQGGSRVAIILKFQLEVSAYATMALREVMKADTSRRSEMCDVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3D3</accession>
    <name>A0A1E3P3D3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_31014</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039203.1">
        <property type="nucleotide sequence ID" value="XM_019181791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3D3"/>
    <dbReference type="STRING" id="683960.A0A1E3P3D3"/>
    <dbReference type="GeneID" id="30199037"/>
    <dbReference type="OrthoDB" id="2414662at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047536">
        <property type="term" value="F:2-aminoadipate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016212">
        <property type="term" value="F:kynurenine-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034276">
        <property type="term" value="P:kynurenic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000024">
        <property type="entry name" value="Kynurenine--oxoglutarate transaminase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051326">
        <property type="entry name" value="Kynurenine-oxoglutarate_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807:SF20">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="6">
        <location>
            <begin position="37"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="425" mass="47933" checksum="CBFA67A7F270CCFF" modified="2017-01-18" version="1">MSTKFPSHNPYFKASGKDVWSLVNEAAAKAASTSTREIINLGQGFFSYSPPQFAINAAKDALDVPAYNQYAHTKGKPNLLKALATTYSKHYGRELSTDEILVTTGANEAILTILTGFLEKGDEVIVFEPFFDQYISNIEIPGGKVVSVPLHPPKEISERNVKGEEWKIDFKELENSITDKTKIIILNTPHNPIGKVFTKEELLKIGELAVKHNILIVSDEVYENLYYTPEFTRIATLSPEIGNLTLTVGSGGKSFAATGWRIGWIIGHPELLKYASLAHTRICFSTPAPLQEATATALNESLENGYFEKTRQDYIKKYEIFTKVWDELGLPYTIAEGAYYLLVDFSKVKIPKDYKYPEDLNGRAKDFKTSYWLIQELGVVAIPPTEFYIKEHEHVAENLLRFAICKDDETLEKAVVRLRELKKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3I8</accession>
    <name>A0A1E3P3I8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial pyruvate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_62407</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the uptake of pyruvate into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039027.1">
        <property type="nucleotide sequence ID" value="XM_019183357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3I8"/>
    <dbReference type="STRING" id="683960.A0A1E3P3I8"/>
    <dbReference type="GeneID" id="30200603"/>
    <dbReference type="OrthoDB" id="869189at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006850">
        <property type="term" value="P:pyruvate import into mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005336">
        <property type="entry name" value="MPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03650">
        <property type="entry name" value="MPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="126" mass="14017" checksum="AB1EC80DB283839F" modified="2017-01-18" version="1">MSSAFARFMASETGPKTVHFWAPVLKWALVIAGINDISRPVEKVSGTQNLSLLATGTIWTRWSFVIKPKNYLLASVNFFLAGTAGYQISRIINYRKSEGDSPREIVNYLINGPQKPKIDTETVIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3Y4</accession>
    <name>A0A1E3P3Y4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Iron-sulfur clusters transporter ATM1, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Iron-sulfur clusters transporter atm1, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_42510</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Performs an essential function in the generation of cytoplasmic iron-sulfur proteins by mediating the ATP-dependent export of Fe/S cluster precursors synthesized by NFS1 and other mitochondrial proteins. Hydrolyzes ATP. Binds glutathione and may function by transporting a glutathione-conjugated iron-sulfur compound.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039270.1">
        <property type="nucleotide sequence ID" value="XM_019182571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3Y4"/>
    <dbReference type="STRING" id="683960.A0A1E3P3Y4"/>
    <dbReference type="GeneID" id="30199817"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140466">
        <property type="term" value="P:iron-sulfur cluster export from the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18582">
        <property type="entry name" value="ABC_6TM_ATM1_ABCB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000004">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000186">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF402">
        <property type="entry name" value="IRON-SULFUR CLUSTERS TRANSPORTER ABCB7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="17"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="340"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ60063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="602" mass="67058" checksum="6E11E916072F0067" modified="2017-01-18" version="1">MSYLWPKDKFSFKVRVVIALSFLILAKLLNVQVPFFFKSIIDGMNIDWTNEVGTVTAIIGGTILAYGGARFGAVLFGELRNAIFATVAQSAIRNVAQNTFKHLLSLDLGFHLSRQTGGLTRAIDRGTKGISYVLTAMVFHILPITFEISVVSGILTYNYGAQFAAVTLLTMLAYSIFTIRTTAWRTGFRRKANNADNQAASVALDSLINFESVKYFNNEAYQAKKYDNALAKYQDYSIKIATSLAFLNSGQNVIFTSALTAMMYMAADGVATGALTVGDLVLINQLVFQLSVPLNFLGSVYRELKQSLLDMETLFKLQKNEILIQNKPGAIPLNLTKGEIKFENVTFGYHPDRPILKNATFTIPGGEKVAIVGPSGSGKSTILRLVFRFYDIQSGRILIDGQDIREVDLDSLRRAIGVVPQDTPLFNDTILENVRYGDLNASDEEVQRVIREAQLDKLIHDLPEGLNTIVGERGMMISGGEKQRLAIARLLLKKSPITFFDEATSALDTHTEQSLLKTIRTIFRENSNTNISIAHRLRTVADSDKIIVLEKGHVREEGDHYSLLQKPDSLYAELWNIQENLDLDEELLANDQEKEKDLTDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P496</accession>
    <name>A0A1E3P496_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH TFE/IIEalpha-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_92606</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIE alpha subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039518.1">
        <property type="nucleotide sequence ID" value="XM_019186373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P496"/>
    <dbReference type="STRING" id="683960.A0A1E3P496"/>
    <dbReference type="GeneID" id="30203619"/>
    <dbReference type="OrthoDB" id="361102at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005673">
        <property type="term" value="C:transcription factor TFIIE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001097">
        <property type="term" value="F:TFIIH-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001113">
        <property type="term" value="P:transcription open complex formation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039997">
        <property type="entry name" value="TFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097:SF7">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH TFE/IIEalpha-type" evidence="5">
        <location>
            <begin position="5"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="267"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="279"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="328"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="369"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="399"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="416"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="429" mass="48830" checksum="DA8C9B0A161A0EE1" modified="2017-01-18" version="1">MDNTVRLLIRFVARGFYGKPYVLILDAVLLHSVLSEDDLSHLLGIQRKELRSLCSRLVEDRLLTVHVQKEEGPQLRPITRTYYFIHYTEAIDSIKWKVHSVVQNIKEDLHMENNPQGYICPVCKTKYSQLDAVALLNYEKNEFLCSLCNEPLIEDDSGKIAKENQVKYSKLMNDLEPIIDYLKKIDERPIPENNYETSLSRVIPAQASSNASYSVSNFKSRKMFSRDSYLNNASSMNAGSRSQATLHVNITTANDDNLQREKQAIAQEEKRKQNALPSWHEESTIGKGLGKLDKEDPEDLDSSEKKSTEDLTNEQAQPETDKDGDVNTEVSTNATTKDTAPSVSTVEDQDKEAQDALAAYYEQLAQKENDEDNDEDEEEEEDDEDEEFDDVDFEDVAGEQNSNEQSTNNEPKAEDLVVEEMEFGESDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4H8</accession>
    <name>A0A1E3P4H8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWIRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_68709</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039387.1">
        <property type="nucleotide sequence ID" value="XM_019184133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4H8"/>
    <dbReference type="STRING" id="683960.A0A1E3P4H8"/>
    <dbReference type="GeneID" id="30201379"/>
    <dbReference type="OrthoDB" id="5598695at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000087">
        <property type="entry name" value="Transcriptional adapter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SWIRM" evidence="2">
        <location>
            <begin position="250"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="347" mass="39604" checksum="F5D42A8BBD6175C9" modified="2017-01-18" version="1">MSSEFSFGAMTPEINSNEAPIIERKSSKSHFIGPKQFHEDQSIPSPPLSPYHSAESSASHNNVLRDIQLKQQNKLFNQGDDKIDTFVVLSNNKTELREFPLDKFENYQLNVNAWSNLGSKNFKRAQINFIRQYTNRAAIKNGHHISKIATRKYAPRRIIRNVHDHVNGGGSSYNSDSDNSSYEKVRTRRLVRESSQALEYSDVSRVSTPPPVKRRKPVSSPSVQPMVIDWESIPDYSPDVSLLPNNSRCLKVEWKGQPMSLADDPLVGKLHPAEVTLASVLRLPCNLYLDSKRRLFAEKVSRLKKGLPFRRTDAQKSCRIDVNKASRLFAAFEKIGWLNDDKFDRFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4R6</accession>
    <name>A0A1E3P4R6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_63011</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="8"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039663.1">
        <property type="nucleotide sequence ID" value="XM_019183475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4R6"/>
    <dbReference type="STRING" id="683960.A0A1E3P4R6"/>
    <dbReference type="GeneID" id="30200721"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000040">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="112"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ60456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="325" mass="37929" checksum="728E0CC489AE0169" modified="2017-01-18" version="1">MTFSNLELDECIERLYRRELLPEVTIRALCFKLKEILVKESNVVYINSPVTVVGDIHGQFYDLIEIFKIGGYCPDTNYLFLGDYVDRGMFSVETITLLICLKIRYPTRVYLVRGNHESRQITQNYGFYSECIRKYGNPNPWYYFTDTFDYLILSVVIDDTIFCTHGGLSPNVHTIDQIKLVDRFREIPHEGAMADLVWSDPDDENPNFSLSPRGAGYTFGVDVVKKFLKMNDMEKILRAHQLCNDGYQIYFKGLVTTVWSAPNYCYRCGNKASIMEIYNKDEHYFNVFEEAPENSEQWGKIDPLTETEIERPQSDIRPQPVEYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4S5</accession>
    <name>A0A1E3P4S5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP-dependent RNA helicase DHH1</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ATP-dependent RNA helicase dhh1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">WICANDRAFT_62974</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039622.1">
        <property type="nucleotide sequence ID" value="XM_019183467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4S5"/>
    <dbReference type="STRING" id="683960.A0A1E3P4S5"/>
    <dbReference type="GeneID" id="30200713"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098562">
        <property type="term" value="C:cytoplasmic side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036267">
        <property type="term" value="P:invasive filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045900">
        <property type="term" value="P:negative regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010603">
        <property type="term" value="P:regulation of cytoplasmic mRNA processing body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000749">
        <property type="term" value="P:response to pheromone triggering conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034063">
        <property type="term" value="P:stress granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17940">
        <property type="entry name" value="DEADc_DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000364">
        <property type="entry name" value="ATP-dependent RNA helicase DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47960">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 17" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="5 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="21">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="60"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="240"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="413"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="431"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="461"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="505"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="521"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00040210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="ODQ60415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="550" mass="61683" checksum="CDFB465997BE8F80" modified="2017-01-18" version="1">MSSDWKQELNIPERDTRPQTEDVTKTKGNSFEDFYLSRKLLMGIFEAGFEKPSPIQEESIPVALAGRDILARAKNGTGKTASFIIPTLEKIKPKIDKIQALILLPTRELALQTSQVVRTLGKHLDVQCMVSTGGTSLRDDIVRLNDPVNVLVGTPGRVLDLAARQVADLSSCSLFVMDEADKMLSPEFKRIIEQILSFLPEKRQSLLFSATFPLTIQEFIARNLKNPYEINLMDELTLRGITNYYAYLEEQQKLHCLHTLFSKLQINQSIIFCNSAKRVELLAKKITELGYSCYYSHARMPQDARNKVFHEFRQGKVRNLVCSDLLTRGIDIQAVNVVINFDFPKNAETYLHRIGRSGRFGHLGLAIDFITWNDRANLFKVEKELGIEIPKIPNNIDESLYVAKSNKNVPKPFPIQNLPNANTNGFHYKGQPELNSQPSSSSIPAPLPLPQQDVPSIGATPPQAAVQQQQQAPSSSGSPAPQQAYPYPPQQANGFTPQGYGYPPQGYPPQGFPQGYPPQGFPQGYIPQQFQQPPQSQQQQFPQQPQPTQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4S9</accession>
    <name>A0A1E3P4S9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_84236</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039570.1">
        <property type="nucleotide sequence ID" value="XM_019185673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4S9"/>
    <dbReference type="STRING" id="683960.A0A1E3P4S9"/>
    <dbReference type="GeneID" id="30202919"/>
    <dbReference type="OrthoDB" id="43460at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045717">
        <property type="term" value="P:negative regulation of fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901352">
        <property type="term" value="P:negative regulation of phosphatidylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001247">
        <property type="term" value="P:positive regulation of phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
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        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052432">
        <property type="entry name" value="PITP/CRAL-TRIO"/>
    </dbReference>
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        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN CSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46590">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="165"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="408" mass="47193" checksum="3EB49857558CA357" modified="2017-01-18" version="1">MTSQSIPPGRIQTLTADEEWKLKEVWAHFLTFWGTPVTLPSGPERTATSTSIASKTSKHEEHGVSTRSNESIEANYSHKVIHDSFKDLQPEDIYSNFWNMLRTDSPDNLILRFLRARKWDSDKALAMLAHTLHWRLKESKVEDLLFGGERRGIENGEEGFHLQFKLSKAYFRGYDNEGRPIVIIRPRLHHASDQTEEEVQKYTLLVIEEARLLLKEPVDSCSVLFDLTDFAMSNMDYPPVKFMIGVFEAHYPESLGKLFIHKAPWIFPPIWNIVKNWLDPVVAAKITFTKSAKDLNKFIPMDYIPKSLGGEDEFEMEYIEPKPEEDELMKDAETRDKLKAERKEIITKFIEATVNWIEAGSVEDSKKYLKEKIELGHELSINYSKLDPYIRARSVFDRIGVIDVQKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4T1</accession>
    <name>A0A1E3P4T1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_105423</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039698.1">
        <property type="nucleotide sequence ID" value="XM_019180445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4T1"/>
    <dbReference type="GeneID" id="30197691"/>
    <dbReference type="OrthoDB" id="3981209at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="309" mass="35011" checksum="D29EBDCA88BA54B6" modified="2017-01-18" version="1">MKRACVEVSSGTACFNRYGQLLVDEMKKHKIYPDAKDANNCLKLVLNGNLKSLSFALEVLTCFIDDSYLAKTTDPSAISKTLMISKVIFVLVLGALCCAIAVRRRKQATLSHFQLNHDQKVETLNQKESKSYTTSFVEETTPEIESLLKIQKYSQAEISIEEEKENDKRLLITSNNQVFDLSTELGKSKWRESVKSQLHKEVTKKDTRIETTSYKQHLFDGNSVLSPRPKATTNASQLGLETNTMDNLYHKRARDDINIHDPTKPVPSILNHPVVDVRGKPLRRIFIPGRGWVSSKTLALEKTRSKRTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4Y6</accession>
    <name>A0A1E3P4Y6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4536 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_62972</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039620.1">
        <property type="nucleotide sequence ID" value="XM_019183466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4Y6"/>
    <dbReference type="GeneID" id="30200712"/>
    <dbReference type="OrthoDB" id="4083608at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053092">
        <property type="entry name" value="Mitochondrial_unc_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28048">
        <property type="entry name" value="ACR195WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28048:SF1">
        <property type="entry name" value="ACR195WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="102" mass="11734" checksum="62F42C5E8F4F84A3" modified="2017-01-18" version="1">MSNIVKVFNPPESRDLEPKEYENCLPCQIMATVTAIGAGIWFTSGQLFDDSKLSKTENLKKNPIWWRYFIRGSGYGLIGYGVFRGTEGWLWNDKPVNDEKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P513</accession>
    <name>A0A1E3P513_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_30556</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039577.1">
        <property type="nucleotide sequence ID" value="XM_019181744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P513"/>
    <dbReference type="STRING" id="683960.A0A1E3P513"/>
    <dbReference type="GeneID" id="30198990"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061673">
        <property type="term" value="C:mitotic spindle astral microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005880">
        <property type="term" value="C:nuclear microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070463">
        <property type="term" value="F:tubulin-dependent ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045144">
        <property type="term" value="P:meiotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099606">
        <property type="term" value="P:microtubule plus-end directed mitotic chromosome migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007079">
        <property type="term" value="P:mitotic chromosome movement towards spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031115">
        <property type="term" value="P:negative regulation of microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070462">
        <property type="term" value="P:plus-end specific microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032888">
        <property type="term" value="P:regulation of mitotic spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01370">
        <property type="entry name" value="KISc_KIP3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000053">
        <property type="entry name" value="Kinesin family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF13">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF19 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="9">
        <location>
            <begin position="11"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="512"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="149"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ60370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="597" mass="67635" checksum="DB824BCFCE44331D" modified="2017-01-18" version="1" fragment="single">MSYPSASRESSVTVAVRVRPFTQSESAHLVVNKEEQVFLGDGALTFNDNAEKENKYRPKRYAPKGLRKIVDVVDDRMLIFDPPENSPLANLSKNVSNHGKRTTRFREHRFVFDKLFDEGTSQEDVYYNTTRPLLDSVLDGFNGTVFAYGATGCGKTHTISGTLEEPGIIFLTMKELFERIDDLKDTKLIDVSLSYLEIYNETIRDLMQPDNGGKLLTLREDASKRINVGNLTSHKPQSVEDVMDMIIIGNRNRTSSPTEANATSSRSHAVLQINVTQKNRTADVKEDHTFATLSIIDLAGSERAAATKNRGERLHEGANINKSLLALGNCINALCDARRRNHIPYRDSKLTRLLKFSLGGNCKTVMIVCISPSSHHYDETLNTLKYADRAKEIKTKVSRNQHSLNRHVGSYLKMITEQKQEIEELRNRESVVVGQAVERYKAKKERVQIGMLEFLDTLKQNLNSKKFQHLKLKKSSILCKRKLLILQRGELQKYLKNIQSLEDPISEFENGYQAIENLVGKLSTKIEELEQLYDEESELDIILSGSANSGIRKLGELEAWSNYDKITLDTTLTLMKDSVERTILYNASMAFDRILSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P549</accession>
    <name>A0A1E3P549_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <ecNumber evidence="2">4.2.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_22286</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="2"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039229.1">
        <property type="nucleotide sequence ID" value="XM_019180934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P549"/>
    <dbReference type="STRING" id="683960.A0A1E3P549"/>
    <dbReference type="GeneID" id="30198180"/>
    <dbReference type="OrthoDB" id="2099276at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000703">
        <property type="term" value="F:oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000005">
        <property type="entry name" value="Endonuclease III-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03183">
        <property type="entry name" value="Endonuclease_III_Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004036">
        <property type="entry name" value="Endonuclease-III-like_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030841">
        <property type="entry name" value="NTH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286:SF1">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01155">
        <property type="entry name" value="ENDONUCLEASE_III_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="13">
        <location>
            <begin position="71"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ60022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="264" mass="30297" checksum="84B0B0844BBD6423" modified="2017-01-18" version="1" fragment="single">RKKTTSLKIELDPKPIPSNFHEIYNHIKTMRSKIITPVDTMGCANIPKTISGEDELTQKNYRFQLLVSLMLSSQTKDEVNFKTMQKMKSYFQSQGFKDGITIDSMERIEERKLDELIFSVGFHTRKASYLKKAAKILKENFDGDIPNTLEGLVSLPGVGPKMGFLTLQEGWGLNLGIGVDTHVHRLSKMWGWVKKDAKDPEVTRLELERWLPKEYWREINPLLVGFGQTICPPRGRRCDLCSLSKTRLCPNVDRQLLKKVKTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P564</accession>
    <name>A0A1E3P564_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Oleate activated transcription factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_79052</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Transcriptional inhibitor with a significantly increased number of target genes in response to oleate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the OAF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039665.1">
        <property type="nucleotide sequence ID" value="XM_019184832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P564"/>
    <dbReference type="STRING" id="683960.A0A1E3P564"/>
    <dbReference type="GeneID" id="30202078"/>
    <dbReference type="OrthoDB" id="5069333at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF33">
        <property type="entry name" value="OLEATE ACTIVATED TRANSCRIPTION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="15">
        <location>
            <begin position="19"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ60458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="912" mass="105124" checksum="8592C759EAAD9F5E" modified="2017-01-18" version="1">MSEVEELKPKRKRQRLTYTCNYCRKRKIKCDKAKPKCSNCQKFGRVCEYEMPYWVNTSVQQNNAVMTDVISENEHQNKTREELYDVINYWKSRSVNNGTNTSTPDFEKKNPELGEHADDIIDLYASYDSLTEKHSSEEDNKPLSTMSVYKADHYMMFFLCFKYITLQIASNSLTKKFEEAGWKSRHPDGATKWLTLLDQKDGDFDLKNSIKRVTNDRSQLFKTNILPFTTIENSDNVQTEALIKDIESILPGKLLLDVYLNFFFRNIWPIRPYVDEELFRQDIKRLITFEDENDPACLKAKVELSQRSDFATLATLLIILRYTSISIQVVDDKDLSLSTLPFRQNLISVSVIPLAQRCLSIYKLLKKSSFPMIQALLYLRVYYKDCPEDGDGLTLGQSQMLFGMIVQSAISIGLHRDPKHYAASTDVRVANAKRRIWYEIVSLDVQTGILSGTLSVVPSSKMTCVKLPVITLGDPLERAQLDELEKEVELQAIHEELVERINRMDEAPTLQKLVELLDNARTYVNENYSMSFMSSISNSEDNSQIYKAAMLRNYKIMEKNLLQICLEITVYHILYIQYEDNKRLDPRMFKHFFKKCLESTTIGFDISSAYLSGALKNYINPVQASFALFPIITTLTYRMVNTVSAALLRSFHAQELLACVFYSTDDCHLNIQAFNEFINSLCNICENHLNIYEQTIGAKYHLSLKVICSCKFSYASLKKHRFSVLNKIVSFLETNDDGKVTDIFWKGAKKQDIRTLFQLNTGMFEVYSQWMQLSKSYTAALKAQYRDHQPVCDVIVNINHSNHFTRLTETDLNEFVEILTGGYRYCSVSASTKNVEQEFSSGVSSASSDSYDSFYDILAQDPMFSAQMSNVLQRSRSNIIDKEMNPDMNDVVNELFQPTTDLLGQFDDLLHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5K8</accession>
    <name>A0A1E3P5K8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Golgi apparatus membrane protein TVP23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_77405</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Golgi membrane protein involved in vesicular trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Golgi apparatus membrane</location>
            <topology evidence="2 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TVP23 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039938.1">
        <property type="nucleotide sequence ID" value="XM_019184552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5K8"/>
    <dbReference type="STRING" id="683960.A0A1E3P5K8"/>
    <dbReference type="GeneID" id="30201798"/>
    <dbReference type="OrthoDB" id="2151161at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008564">
        <property type="entry name" value="TVP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019:SF7">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05832">
        <property type="entry name" value="DUF846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="223" mass="25094" checksum="66C0F588B0B00368" modified="2017-01-18" version="1">MTVYTDSSGVIWYNGFSETYRERKQLEEMSETTGIAGPPPESRTLFQRLSESSHPIALIFFLGFRIGSLVAYLLGLLFTKNFIFFFISVILLLAADFWNVKNISGRLLVGLRWWNETDDNGESIWVFETSDPSRYINPIDSKVFWILLYASPAFWIALAVFAVLKFEFLSLILVIIAVALTMTNALAFTKCDKFGKANSIASDVFGRVSGGFLSSITPSFLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5S1</accession>
    <name>A0A1E3P5S1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_28773</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039930.1">
        <property type="nucleotide sequence ID" value="XM_019181537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5S1"/>
    <dbReference type="STRING" id="683960.A0A1E3P5S1"/>
    <dbReference type="GeneID" id="30198783"/>
    <dbReference type="OrthoDB" id="7042322at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047536">
        <property type="term" value="F:2-aminoadipate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000080">
        <property type="entry name" value="Aminotransferase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42858">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42858:SF1">
        <property type="entry name" value="LD15494P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="1">
        <location>
            <begin position="46"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="425" mass="47603" checksum="55DF12A20718202D" modified="2017-01-18" version="1">MSINFFKGHPTESLLPNSTIVNATTSLLTAKRDYDLDPQNRHPLTYGSDAGAIWVRDEVAKFSNSTFKPKSPTTHDFINLTAGSSYGIMNILTQTTLPHTNYTKQAFLITPTYFLINETFIDAGFGGKMTGIDEIPGGVDLVQLESKLKQFNGSDSNDEDLKLINNPQGVPKKIYKFVLYCIPTFSNPGGETYDLETRLKLIELARKYDMLIITDDVYDLLDYEQSLEVLPQPLPRLTHLDRDSYGNEYGNTIINSTFSKLIAPGLRFGYQESVNEKLVHQLSQGGANVSGGTPSQLNSMVVGTILQNGDIEKVINSFRRVYSERAHLLQKLIKDLLPSGTKATGFNGGYFIWVTLPSNYDVGEICQKLKNDENIVLANGDNFEVVGDIRHWGKRSVRLSISYLTKDEIQKGIEQWAKVIKEMYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5S5</accession>
    <name>A0A1E3P5S5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_51305</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039885.1">
        <property type="nucleotide sequence ID" value="XM_019182666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5S5"/>
    <dbReference type="STRING" id="683960.A0A1E3P5S5"/>
    <dbReference type="GeneID" id="30199912"/>
    <dbReference type="OrthoDB" id="5784at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070916">
        <property type="term" value="C:inositol phosphoceramide synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016772">
        <property type="term" value="F:transferase activity, transferring phosphorus-containing groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006676">
        <property type="term" value="P:mannosyl diphosphorylinositol ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03386">
        <property type="entry name" value="PAP2_Aur1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026841">
        <property type="entry name" value="Aur1/Ipt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052185">
        <property type="entry name" value="IPC_Synthase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310:SF8">
        <property type="entry name" value="INOSITOLPHOSPHOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14378">
        <property type="entry name" value="PAP2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="6">
        <location>
            <begin position="192"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="376" mass="43012" checksum="DBC7FCA4450549C5" modified="2017-01-18" version="1">MFKVITNIPVFFVKFIIKIIKSGLNQRNIFQLALNFFINFSPVAIWLTIFKNAGLIPNEIRPKIYVDLIYNLDLEIFDIDFINVLIWSIISLSFTYLCSEFDLSDSSSSSSSGSISSSLSISSKAITRTHSLYITPILFFSIWPILNIASHFASNITTTKDLIAWFFYVLGHITFPILTAIYLYVFQPQGALKFFSIALGMQNIAGVLTHLLFPNAPPWFIHMYGPDASADYDMPGYAAGLTRIDVALGTHMHSKGFHKSPIVFGAFPSLHSAMAVQVFLFIFYFARFRILRLAALLFVYIQWWGTIYLDHHFKIDLFAGCGYALISFAIVYPFLKKKEHDYIKARLNNDFKKGSTMGMRVFEKIPKLQRFFDPYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P663</accession>
    <name>A0A1E3P663_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_77418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039951.1">
        <property type="nucleotide sequence ID" value="XM_019184554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P663"/>
    <dbReference type="STRING" id="683960.A0A1E3P663"/>
    <dbReference type="GeneID" id="30201800"/>
    <dbReference type="OrthoDB" id="5563016at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="465"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="580"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="185"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="212"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="326"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="398"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="513"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="586"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="798" mass="88471" checksum="22FDEEA5EC1BD198" modified="2017-01-18" version="1">MSTISTANHSVPRGNSTNDLLFMESRFTKPSRSKLQHYQYHPNVQNGYVIQPRPLPRASSVPSIPQFSIQNGQRPSMPKSQTQQLYPNLRPSTSMNHIPAYRRNSLKSNSIQMNGVVGINTQHSSYTQPSNMGQKRRHSAKPTASFTFPNGEVYTPRNKQRGSQTSLATLHRLQTAKHFQGGIQSSPSLVSPPLSFSASQQHITSVRPPQQHAHSISSSATPTTAITTPTTSPPATNVQTAEQDLSLLTTGSNSYSSSPSPSRTSRTNSSVNESTPPSSIDDLTIHTTNKADLSQLPPPLQHFEVTPLNNLGKIEEHAVIDQEVKTTTSTPTSESTQKTLEAQAPIESTTIENSVNNSKETLNLQEDVDPKEPDPVYLGSVKGSKDAEPPKPSPVPVSAPVSSSPAPEIKFQTVPKTQVKSSPAPVVLQTPLTSKLASKVSSANPNTKVERKQSLLKRIFSKVFSSDHKKKSKSIKRKNDEKVEVKTPHFNPPARRSHPNEVVKESQPVKPVTTPSVAVVNTSHTLNPEVDTSDVFSFVDGDEDGGIKVNTPEEEEHDSDSSDFKSELVLDKLFSRLSTNEPSEQVYKKITDEKKKRKEEPKSQKTDMKAFTPAPVDDEDEIIQFDDMELIDKIIEFGETPFPDLASTDLGQSQRKLQRSKSIERKKSIRSISSSSARQLDDVITQSVPTIFVSKQPNLEVIYSNQPKDPFPLIQTRPSILKKKSLEITPSTKKVGFTNQIFVNNTYPSYVYNRHSRSLSSYSLSPQLIHQIRHELNDFKRSMTVHEMSKGNTHYFRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P666</accession>
    <name>A0A1E3P666_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Mannosyl-oligosaccharide glucosidase</fullName>
            <ecNumber evidence="11 12">3.2.1.106</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Glucosidase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_67453</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(4)-(alpha-D-Glc-(1-&gt;2)-alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + H2O = N(4)-(alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:55988"/>
            <dbReference type="Rhea" id="RHEA-COMP:12806"/>
            <dbReference type="Rhea" id="RHEA-COMP:14355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:59082"/>
            <dbReference type="ChEBI" id="CHEBI:132537"/>
            <dbReference type="EC" id="3.2.1.106"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="13">Glycan metabolism; N-glycan degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the glycosyl hydrolase 63 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.106" evidence="11 12"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039987.1">
        <property type="nucleotide sequence ID" value="XM_019184083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P666"/>
    <dbReference type="STRING" id="683960.A0A1E3P666"/>
    <dbReference type="GeneID" id="30201329"/>
    <dbReference type="OrthoDB" id="410058at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098553">
        <property type="term" value="C:lumenal side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004573">
        <property type="term" value="F:Glc3Man9GlcNAc2 oligosaccharide glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070880">
        <property type="term" value="P:fungal-type cell wall beta-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.110">
        <property type="entry name" value="Glycosyl hydrolase family 63, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031335">
        <property type="entry name" value="Glyco_hydro_63_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031631">
        <property type="entry name" value="Glyco_hydro_63N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038518">
        <property type="entry name" value="Glyco_hydro_63N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004888">
        <property type="entry name" value="Glycoside_hydrolase_63"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412:SF11">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03200">
        <property type="entry name" value="Glyco_hydro_63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16923">
        <property type="entry name" value="Glyco_hydro_63N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 12 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133685" description="Mannosyl-oligosaccharide glucosidase" evidence="14">
        <location>
            <begin position="19"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 63 N-terminal" evidence="16">
        <location>
            <begin position="37"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 63 C-terminal" evidence="15">
        <location>
            <begin position="304"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ60780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="812" mass="93069" checksum="F8004889631E87B6" modified="2017-01-18" version="1">MLRNFLLLLTALLTVVSGLDVDKEQLIKDYQNAIDQSLLWGPYRPNAYMGIRPRIPHSLVSGLFWFNADDIQNIHKIRHFCDNGADFQGFGWTKYDPRLGGRQEFKDNELKLDITTDFVKSKDGNWAMKVKGVPRPGYESQTNSIVFYAGLEGEGMLTSASGEPPEGFKKGDSIKLLGHSIDFQGFEIDVSDGPKTNLHKSLKKSQLADPALNPARTHYLSMRVPDGNVWKAKDIFNVLISESVNDIAAKNPDAKVMPHPSSLMSLRNINGFEGNLHFIQKTFTGAFEFDITFNIENADKRLEFEDIDELTNKTISKFENKFNKVFDLQPPFNKPKYQKFAEEFLSNIMGGIGYFYGDHLVDRETEFNEEKYENIQLDGKPEGPFELFSAIPSRTFFPRGFYWDEGFQLIPILQYDTDLVLEILKSWFNLIDEDGWIAREQILGAEARSQVPVEFQVQNPNIANPPTLMLLFTDLLNKAKEAQEGSADLGDIDEPETVDAYGKVDFTELTDAHLKYPDLLIDYAEKIYPKLQSHYEWFKRTQRGELEEFEREPSSPVEAYRWKGRTFTHNLPSGLDDYPRAQPPDIAELNVDLISWVGTMTRSMKKIAELLGKKEDAAKYSKVEYAIQRNIEDLHWSEKDKAYCDVSVDQADNDVFYCAKGYISLFPFALKMLAPGNKHIRDVIELIADPEELFSPFGLRSLSKSNEFYRTDEDYWRGPIWLNINYLVLDSLLHYGTGENAKQLDQDTFALANKTYTELRKNLVNNVYRQWRATGFAHEQYDDKTGKAKGAKHFLGWTGLVTVIMRMPESLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6D8</accession>
    <name>A0A1E3P6D8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartate aminotransferase</fullName>
            <ecNumber evidence="7">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_29401</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="7"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039719.1">
        <property type="nucleotide sequence ID" value="XM_019181601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6D8"/>
    <dbReference type="STRING" id="683960.A0A1E3P6D8"/>
    <dbReference type="GeneID" id="30198847"/>
    <dbReference type="OrthoDB" id="6752799at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006532">
        <property type="term" value="P:aspartate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF55">
        <property type="entry name" value="GLUTAMATE OXALOACETATE TRANSAMINASE 1, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="8">
        <location>
            <begin position="29"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="402" mass="44351" checksum="7516DD3652732332" modified="2017-01-18" version="1">MSSFTNLQFQPADEFNDLRKKLALDSNENKVDVSAGVYRDETGESYTLPSIRAAKKILHESGKGHDYTFCLGSSDFLEPAAQLAVGNDAVKKGLVSSCQTIGGTGACHLGATFLAQYCGFGEFYLGTPAWPNYFPLLKQVGGSVHTFDYYDSENKSVDFKVLMQTLENAPQYSVFILQLCCHNPTASDLSIEQWKKVGEIMKAKSLIAFIDAAYQGFASGSIEKDGEPIRIFIELGVEVLVAQSFSKNLGLYGERVGCLHVVSTDPMVTPVITDQLRYILRAEASSSPAFGSRLVSIIANSDDLSAQWGTDVAEISKRLQTLRAKVYDLLTEKYQTPGSWDHVKSQRGLFWYSGLTQEQAEKLINVYHIYLTKGGRINIAGLNEKNIDYFCSSLDKVVRSVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6N2</accession>
    <name>A0A1E3P6N2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Small nuclear ribonucleoprotein Sm D2</fullName>
            <shortName evidence="12">Sm-D2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 12">snRNP core protein D2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_83313</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the snRNP core protein family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040286.1">
        <property type="nucleotide sequence ID" value="XM_019185523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6N2"/>
    <dbReference type="STRING" id="683960.A0A1E3P6N2"/>
    <dbReference type="GeneID" id="30202769"/>
    <dbReference type="OrthoDB" id="437526at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Sm_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000018">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027248">
        <property type="entry name" value="Sm_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12777">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="13">
        <location>
            <begin position="30"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ61079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="113" mass="12965" checksum="C073CD5B5268BE08" modified="2017-01-18" version="1">MAKFMDRPKSELTDIELQKLEEYEFTHGPMSILQDAVQNGTQVLISCRNNHKLLAKIRAFDRHSNLVLENVKELWTETPRNSKGKKGKPVPKERFVSKMFLRGDSVIVVLKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6W7</accession>
    <name>A0A1E3P6W7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_29556</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040247.1">
        <property type="nucleotide sequence ID" value="XM_019181626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6W7"/>
    <dbReference type="STRING" id="683960.A0A1E3P6W7"/>
    <dbReference type="GeneID" id="30198872"/>
    <dbReference type="OrthoDB" id="5982228at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF382">
        <property type="entry name" value="LOW-AFFINITY METHIONINE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="432"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="466"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="505"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="61779" checksum="33E9843A36F12C8F" modified="2017-01-18" version="1">MSSSKDLLKDSITTVEVNSRSNSDASEDQLLSPVHSQRSYGSLHKELLNDSILQIDLEDLPHGRHLGLYSTIILFISRIIGSGIWATPSNVFAGVGGNPFLFFTMWLIAALMAFAGLYAYLELGSIVPRSGGTKVFLEFIYPRPKLLISVVFSLFTLAFGISLTNSIVFGKYTLFSLGYSSEFIDSSSWSNYIGLILIIVTTLIHGFSTKHGVFVQNVLGAMKLFLVAIMSLTGIYVLCFPEKLTGLPNHLDMDKFFKFDDSSIVTASSVSSSLLGCFFTFGGWNAVHTVSSEIKDPVRTLKIAGPVSLLIAFFNFILINIAYLKVIPHDEVIKAGPLIGSILFERVLGYRLGRQFLTISIALSSASNVFVVVYSISRMNQEIFREGYLPFSSTFASNFRHTNTPLWSLLVCSALTSFWLILLPANGKSFNYIVSLEGYSTQVFLLLIAIGIFIIRKKYPDQKAPIRASITGTALIVVVSLSVLISPFFSKNDSSNPTYISYLPPYYIASPFLLSLGLAFWAIKFKVLPYLGSFKYHREIETLDDGLTVKKWVKVHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P743</accession>
    <name>A0A1E3P743_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Smr domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_23927</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040317.1">
        <property type="nucleotide sequence ID" value="XM_019181007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P743"/>
    <dbReference type="STRING" id="683960.A0A1E3P743"/>
    <dbReference type="GeneID" id="30198253"/>
    <dbReference type="OrthoDB" id="3231855at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013899">
        <property type="entry name" value="DUF1771"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053020">
        <property type="entry name" value="Smr_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47417">
        <property type="entry name" value="SMR DOMAIN-CONTAINING PROTEIN YPL199C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47417:SF1">
        <property type="entry name" value="SMR DOMAIN-CONTAINING PROTEIN YPL199C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08590">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01713">
        <property type="entry name" value="Smr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01162">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00463">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SMR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Smr" evidence="2">
        <location>
            <begin position="76"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="151" mass="16893" checksum="A96F88D0056C7F52" modified="2017-01-18" version="1" fragment="single">SDPEYKRLREQADLAYKKKQQLSSESQKAYKNGDGQRAHELSEAAKKQVDIAENYNFQAAEYVFVANNADSDSDEIDLHGLYVKEAQWVLKKRIVAGIQRHESKLEVIVGKGLHSQNGVAKLKPAVEELCDEAGFKSHIDPKNTGVLVINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7K5</accession>
    <name>A0A1E3P7K5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E3 ubiquitin ligase complex SCF subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_104453</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SKP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040580.1">
        <property type="nucleotide sequence ID" value="XM_019180415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7K5"/>
    <dbReference type="STRING" id="683960.A0A1E3P7K5"/>
    <dbReference type="GeneID" id="30197661"/>
    <dbReference type="OrthoDB" id="2342932at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031518">
        <property type="term" value="C:CBF3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043291">
        <property type="term" value="C:RAVE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010458">
        <property type="term" value="P:exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000766">
        <property type="term" value="P:negative regulation of cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010828">
        <property type="term" value="P:positive regulation of D-glucose transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007096">
        <property type="term" value="P:regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043254">
        <property type="term" value="P:regulation of protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18322">
        <property type="entry name" value="BTB_POZ_SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000133">
        <property type="entry name" value="Suppressor of kinetochore protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016897">
        <property type="entry name" value="SKP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036296">
        <property type="entry name" value="SKP1-like_dim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016072">
        <property type="entry name" value="Skp1_comp_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016073">
        <property type="entry name" value="Skp1_comp_POZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11165">
        <property type="entry name" value="SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01466">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03931">
        <property type="entry name" value="Skp1_POZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028729">
        <property type="entry name" value="E3_ubiquit_lig_SCF_Skp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81382">
        <property type="entry name" value="Skp1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SKP1 component POZ" evidence="5">
        <location>
            <begin position="4"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="SKP1 component dimerisation" evidence="4">
        <location>
            <begin position="112"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR028729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="161" mass="18643" checksum="F06AEFE30FA3FFFC" modified="2017-01-18" version="1">MSEVTIISSDNESFKVERKIAERSVLIKNMIGDLANDDDDDFEIPVPNVRASVLKKVLEWATHHKDTSFPDDEDEDSRKSAPVDEWDKEFLKVDQEMLYEIILAANYLNIRPLLDAGCKVVAEMIRGKSPEEIRRTFNIVNDFSPEEEEAIKRENEWAEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7N6</accession>
    <name>A0A1E3P7N6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Box C/D snoRNA protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Zinc finger HIT domain-containing protein 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_78068</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for box C/D snoRNAs accumulation involved in snoRNA processing, snoRNA transport to the nucleolus and ribosome biogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with FBL, SNU13, NOP58, NUFIP1, RUVBL1, RUVBL2 and TAF9. Interacts (via HIT-type zinc finger) with the RUVBL1/RUVBL2 complex in the presence of ADP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the BCD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040646.1">
        <property type="nucleotide sequence ID" value="XM_019184655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7N6"/>
    <dbReference type="STRING" id="683960.A0A1E3P7N6"/>
    <dbReference type="GeneID" id="30201901"/>
    <dbReference type="OrthoDB" id="272357at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070761">
        <property type="term" value="C:pre-snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016074">
        <property type="term" value="P:sno(s)RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048254">
        <property type="term" value="P:snoRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23023">
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        <property type="entry name" value="box C/D snoRNA protein 1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="BCD1"/>
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        <property type="entry name" value="BCD1_alpha/beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13483:SF3">
        <property type="entry name" value="BOX C_D SNORNA PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13483">
        <property type="entry name" value="BOX C_D SNORNA PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25790">
        <property type="entry name" value="BCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51083">
        <property type="entry name" value="ZF_HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HIT-type" evidence="15">
        <location>
            <begin position="12"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="251"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="267"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="299"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061949"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00068630"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00077531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ61439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="327" mass="37644" checksum="1939FF0D10F75E3D" modified="2017-01-18" version="1">MSGAIEELKELCQICYEQKFKYKCPKCGTKTCSLECVNKHKTQSQCDGVIDNAKFVKRSEFKENENLVHRDYNFLMKMNRTIEVSKNDVRSKNKKILSNYHNNNKRFQRDSASNNYESIVKRGVKIRKLPRGMQRNNMNKSGWDQKKDTYVWTIEWILIDNETGSELAKHFSFRVPEGTKLEEAIHQTIKEKVGDVPVFCFLKKIDTPASNPQFIPLDNEHLLADVLRDQTVIEFPTILVAKEKEVKGYTVYESEESESESSSSEESSSEESSSEEDSEPEEVSSKQQDDTENSEQNTEEVKAKEEETKDPIADSTDELGIKETTAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7S8</accession>
    <name>A0A1E3P7S8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EXS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_61926</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183278.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3P7S8"/>
    <dbReference type="GeneID" id="30200524"/>
    <dbReference type="OrthoDB" id="2159384at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF46">
        <property type="entry name" value="PROTEIN ERD1 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="304"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="6">
        <location>
            <begin position="186"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="393" mass="46193" checksum="37043BE64B7805FA" modified="2017-01-18" version="1">MPQETDIFDNILPLPYRVISLIVLGSWLWLLTLKISNNVGINITSLLKIDESPTSTNLTIAQIMHNHEKSSINISLLFVTSYIIFTLFHDQNIESLTIFDFLPLLTIMAIFYTLLKPNNLGSKRLLSSFKRILKGNIDQSIRTNDILLSDTLTSYSKILIDFAIYMCHIINFETCLPKSKGSTINRSCGDSILLESLVGVIPTLIRLRQCFIEYKISNQRNKTHLLNFIKYSTNIPILIISLIIKIKKTDKLTKIWVLASLINSTYSFIWDVNNDWDLKFFQKFIYGKNDHGVLRLKLHYDFKLFYYIAILLDFQFRYIWVLKFSPKYENANLFWNFLSCLYTTEFGLYCLQTIEIFRRWIWVFIKVEVDFINMNPLEYRKDHDSIELDSLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P963</accession>
    <name>A0A1E3P963_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_52546</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040624.1">
        <property type="nucleotide sequence ID" value="XM_019182689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P963"/>
    <dbReference type="STRING" id="683960.A0A1E3P963"/>
    <dbReference type="GeneID" id="30199935"/>
    <dbReference type="OrthoDB" id="436519at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052094">
        <property type="entry name" value="Pre-mRNA-splicing_ERAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44313">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44313:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="15"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="89"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="314" mass="36274" checksum="0B2F694ECE1C3C1F" modified="2017-01-18" version="1">MSSKDIDYLKSNNVDIYQLLEVPNDAQDVTIRKAYRKQALIYHPDKNPSPSAAEKFHSISLSLKVLTDKHLRSEYDNWLSSKKLESLRTEKLDASRRKMKDDLERAENDASSQGFTWNKRAATESANRFGAELERLRQEGAQKRREFEKQYLSKFDSDEVSKRTKSSVANDSTQLDFTVKVRWKIKEGISDLFNADILSGIMSVFGEIESANILPRGSESRYDTGLVKFKSKSCAIEAVNHDFSRKSSKWEGTSYRKLSGLLREAKWNKKQPKNIPNDGILNKDEMSFEEYMNLTLLKLKEAKDPKHLRRKFEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P970</accession>
    <name>A0A1E3P970_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_27625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041166.1">
        <property type="nucleotide sequence ID" value="XM_019181394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P970"/>
    <dbReference type="GeneID" id="30198640"/>
    <dbReference type="OrthoDB" id="4138121at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020301">
        <property type="entry name" value="Mrx7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10906">
        <property type="entry name" value="Mrx7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="81" mass="9822" checksum="59681E968D1416A5" modified="2017-01-18" version="1">MKSPRNLEEFLYLKLMNSPAFHRFVRNIYFKVNGIQPPTRPLHEQHAADHLFQPTSAQKFKAYRLLFYDEWRATFGFPRKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9B7</accession>
    <name>A0A1E3P9B7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_59891</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041021.1">
        <property type="nucleotide sequence ID" value="XM_019182897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9B7"/>
    <dbReference type="GeneID" id="30200143"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="131" mass="13649" checksum="C5D08126B6184BC3" modified="2017-01-18" version="1">MIFPSLIVLPPDFSSLSITYTAVNKELIFILRLLYLHFKMDSAANAAMNAKMAANNAANRAMHGAMAANKAANREMFSAMQGAGQSSGGGGLGSIGVPVAGAALGFMFPGSSLVYYGCMAATAYSFAGWFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9D4</accession>
    <name>A0A1E3P9D4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="5">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_49222</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="5"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041230.1">
        <property type="nucleotide sequence ID" value="XM_019182628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9D4"/>
    <dbReference type="STRING" id="683960.A0A1E3P9D4"/>
    <dbReference type="GeneID" id="30199874"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF937">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="8">
        <location>
            <begin position="80"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="7">
        <location>
            <begin position="208"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="513" mass="57963" checksum="D64D9683490A0531" modified="2017-01-18" version="1">MSSKTPLPFSPETPTTSTSSTPIISKQQCITLKPCSHLSQVLNSNAKDTVIRNYGLAAKVSSFNTISSLNMPSFVNIKGNKSKVDRHKILKLKTRAVKCRDCSTNLGNTFMCLQCPHVGCWHKHHFIKHAKLAGHIFGIDGQLGYLFCFKCGDYTNDPQLEAVRLNMIGNHNSTYELQHTDNQTEEENEKLITEYSRPTTFKATTGLRGFVNMGSTCFMSSVLQTFIHNPIIREFFMTEGHMDCSKQKNECITCCVDEIFTDFYTTNKTSGFGPTSLLNAAWKTNKSLAGYSEQDAHEFWQFLVNQLHHDLTGANNHNSSSCDCVVHKAFSSDLQSAITCLECGNVTKTIDPIIDLSLEISDNSGLIDCLGNFTKSENLDIKYSCSNCQTRTNATKKLTILKFPNVLSIQLKRFKHNNQSVKIESFIEFPMFINMSEFATNYEVDKKQNPYLNYELFGVVCHIGSVNTGHYVTVIKNNEGKWFKFDDSVVTLMTWDQVSQLKAYMLFYMIHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9I0</accession>
    <name>A0A1E3P9I0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">dihydroxy-acid dehydratase</fullName>
            <ecNumber evidence="14">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_87223</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:27695"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IlvD/Edd family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="14"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041273.1">
        <property type="nucleotide sequence ID" value="XM_019186098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9I0"/>
    <dbReference type="STRING" id="683960.A0A1E3P9I0"/>
    <dbReference type="GeneID" id="30203344"/>
    <dbReference type="OrthoDB" id="3851628at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00057"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.80:FF:000001">
        <property type="entry name" value="Dihydroxy-acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="entry name" value="IlvD/EDD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050165">
        <property type="entry name" value="DHAD_IlvD/Edd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056740">
        <property type="entry name" value="ILV_EDD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="ILV_EDD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002068">
        <property type="entry name" value="PRK00911.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21000">
        <property type="entry name" value="DIHYDROXY-ACID DEHYDRATASE DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21000:SF5">
        <property type="entry name" value="DIHYDROXY-ACID DEHYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24877">
        <property type="entry name" value="ILV_EDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="IlvD/EDD N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroxy-acid/6-phosphogluconate dehydratase N-terminal" evidence="17">
        <location>
            <begin position="58"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Dihydroxy-acid/6-phosphogluconate dehydratase C-terminal" evidence="18">
        <location>
            <begin position="394"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF24877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ62066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="593" mass="63343" checksum="32FDEF1212324ED6" modified="2017-01-18" version="1">MSMLSHLASGSKRSFSTSSRQCQKAYNKYSKIITQPKDQGASQAMLYATGFEQEDFNKGQVGVGSVWWSGNPCNMHLLDLNFRCSQSVNNAGLKAMQFNSIGVSDGISMGTAGMRYSLQSREIIADSFETIMMAQHYDANISIPSCDKNMPGVLMAMGRHNRPSIMVYGGSIKPGHPTCGSASIPENIDLVSAFQSYGQYISKQISEEERKDIVQHACPGAGSCGGMYTANTMASAAEVLGMTLPNSSSSVAVSEEKLAECDNIGEAIKKIIDLDIRPRDIMTKEAFKNAITYVIATGGSTNACLHMIAVAHSAGVKLTLDEFQAISDKTPLLADFKPSGKYVMAELAQYGGTQAVLKYLLNEGFMNPDTLTVTGKTLGENLATNPGLPEGQPIIYPVSNPIKANGHLQVLYGSLAPGGSVGKITGKEGTYFKGKARVFEEENAFIKALENGEIKKGEKTVVIIRYEGPKGGPGMPEMLKPSSALMGYGLGKDVALLTDGRFSGGSHGFLIGHIVPEAAQGGPIGLVKDGDEIVIDSDNNKLDLLVSPEELAERKSKWVAPQPRYTRGTLSKYAKLVSDASHGCVLDADEVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9I6</accession>
    <name>A0A1E3P9I6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar import/degradation Vid27 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_26974</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041167.1">
        <property type="nucleotide sequence ID" value="XM_019181323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9I6"/>
    <dbReference type="GeneID" id="30198569"/>
    <dbReference type="OrthoDB" id="10251113at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040458">
        <property type="entry name" value="Vid27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013863">
        <property type="entry name" value="VID27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040979">
        <property type="entry name" value="Vid27_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040768">
        <property type="entry name" value="Vid27_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913:SF0">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08553">
        <property type="entry name" value="VID27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17748">
        <property type="entry name" value="VID27_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17747">
        <property type="entry name" value="VID27_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vid27 N-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Vid27 PH-like" evidence="3">
        <location>
            <begin position="252"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar import/degradation Vid27 C-terminal" evidence="2">
        <location>
            <begin position="441"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="797" mass="89613" checksum="73824007E65FFF2D" modified="2017-01-18" version="1">MNFLKKFLGSSPKDDLSMIPSGQLFLKRSLGSPKSASECLYTDAAASVRETSAPHNYQLVIQRAFAEGEEQLKGDDDEEEDDFAEFQDERVFLIDKSLKIHHFNRSGSLIVSWSNRDGDDGERFEFVVDESIKSSEVEHFMKTVYCCEFERKYSKSSAGVTTEQLDEFVFDPESESIEATLSKSIGNTPTKNLLSVTSEDEGEDSIYEDAEEVTGAAATSPIPSRISQPATPKKDDLAKKEVEQVKEPTGEEIVQVAAELRLYDPTTESFSLQDTDGTVTVLDLGKWEYWLSIKSKSGISLGTLISPKLNPVFNYEVVSFIFNYETGDDEVFSWLLRFEAPQELLEFQTGYMRAAWEAANKSKWQKAEASEKQYVLDAFNTNEEDQDMADYPEEEEEEEEEEQEKSSRAVNLRTGLRKEGFSDSEDEEEDEVKKNYFKGSQNKNLTIAFKNDRSYVTRGNRIGVFKTTDDDDLEFATAIENLSIGDKKQFSPTKMMLHTEDRSLVMQGDKKDTLYRMDLEYGKIVDEWKVKEDLPVVDFGPSKKFNQMTGEQTFLGISDKGLFKVDPRVNGDKLVESEYKTYATKNGFSSFGTTEDGFIAVASDKGDLRLYDRLGIRAKSLIPAIGDSIKFVETSADGKWLLATCQTYLILIDLTIKSGANAGSLGFKKSFGKDNVPKARTLKISPEHVASIKQLTGTPLNFSKAHFNTGINAKEQTIVSSSGPYAITWSLRKILRGDTEPYMIKRYSSNVVADNFKFGTDKNVIVALDDDVGIVNKKAFRKADRQTLAYVPREGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9J6</accession>
    <name>A0A1E3P9J6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">4-nitrophenylphosphatase</fullName>
            <shortName evidence="5">PNPPase</shortName>
            <ecNumber evidence="3 5">3.1.3.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_26719</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 5">
            <text>4-nitrophenyl phosphate + H2O = 4-nitrophenol + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21664"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57917"/>
            <dbReference type="ChEBI" id="CHEBI:61146"/>
            <dbReference type="EC" id="3.1.3.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Divalent metal ions. Mg(2+) is the most effective.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.41" evidence="3 5"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041177.1">
        <property type="nucleotide sequence ID" value="XM_019181295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9J6"/>
    <dbReference type="STRING" id="683960.A0A1E3P9J6"/>
    <dbReference type="GeneID" id="30198541"/>
    <dbReference type="OrthoDB" id="413953at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000039">
        <property type="entry name" value="Phosphoglycolate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006349">
        <property type="entry name" value="PGP_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01452">
        <property type="entry name" value="PGP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF46">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000915">
        <property type="entry name" value="PGP-type_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="25"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00050247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00066659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00069197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="296" mass="32293" checksum="19575B30F71D6E10" modified="2017-01-18" version="1">MSPTKITSKEQVSQLLQNYDTFIFDCDGVIWLGDHAIPGAVESIELLTSLNKQVIFVTNNSTKSRKAYTKKFEKFGLKVSKEQIFGSAYASATYLHQFIKLPLDKKVWVMGESGIEEELKELGYTSIGGTDARLDEKFDANTTPFLPKDSSVGAVITGLDTKVNYHRLAITLQYLQDPNVEFLATNIDSTFPQKGLILPGAGSIIESVAYSSGRESIACGKPNQNMLDAIVADKGLDRSRAIMVGDRLNTDIKFGNDGGLGTLLVLTGIETEDNVLKTGAPKFYADSLGVIGELYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9L7</accession>
    <name>A0A1E3P9L7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_82212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041310.1">
        <property type="nucleotide sequence ID" value="XM_019185350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9L7"/>
    <dbReference type="STRING" id="683960.A0A1E3P9L7"/>
    <dbReference type="GeneID" id="30202596"/>
    <dbReference type="OrthoDB" id="10692659at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="446"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="561"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="652"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="652"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="732"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="743" mass="83236" checksum="43C553475006159D" modified="2017-01-18" version="1">MLKAIETAEKIEALGEDFVDDDSKPQVKRSLFNPGLTRKDENRPILDYTKTAKETPNSSGLKSESEPEKGTRKRKFEDVLDEHERKDDENFRGMFGKVRKFARFVTRELSTTLDSPISDQEIGNELEDKTRDGVENQSANSDVVENKTAQVENEPSHNFIQSKLDAVGETLVEAENTIEEFTSFVEEQVEDLVEDPVEPAEENVTHIQSSLVVDFASKAEAVADEFGNFVEGQVEELIENPVEPAEANVDDTHTTISDTVAKTEVIAEELGDFIKDKVEELVEHPVEPVEENIEPNEPIKETIKMAENIVEDFGGFVEEKVEELVNNPVDPVEGAITNKTTSVGHAISEAENVVKDFEGFVHEEVKELVENPVDDEVDEAPAETSVSQAAEVVGDFGNFVQDQVRVAIEEPVDEMCQTINQPTEPKDEILGKLNDAAHVLEEKVEEVIDNPVTDEPDHATSLEDEAGVENLEARDFEVDEDLAADNMEDSGNNTDKEHGDVIVEKTEDTKEEPERGSKTNHNTNEEDLEKQPQLNPDDVNISQAKSPLKKVFDYFFKSSSKNDGLEATAPENDNPTSSPVERPPKRRKKTAVTDFHDHIVMKTRSGKIIGANSDADIADAQDLEQEEEELIDRIAHGEVHTGHVLHELALEHERLEHEHEHGPFNENEQKVEEMETKEETEQKVEELETKEESITDKKSKESNVENPETIKDEKGSKLDNPKQRTRGKKRSRTDGDLDEGSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA97</accession>
    <name>A0A1E3PA97_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1748-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_60397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041543.1">
        <property type="nucleotide sequence ID" value="XM_019182983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA97"/>
    <dbReference type="STRING" id="683960.A0A1E3PA97"/>
    <dbReference type="GeneID" id="30200229"/>
    <dbReference type="OrthoDB" id="16824at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013726">
        <property type="entry name" value="Mitofissin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28075">
        <property type="entry name" value="CHROMOSOME 16, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28075:SF1">
        <property type="entry name" value="DUF1748-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08520">
        <property type="entry name" value="Mitofissin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133748" description="DUF1748-domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="73" mass="7749" checksum="AB4BC21F9754900C" modified="2017-01-18" version="1">MGILGKAIHLSVDLVLVSTALAGIKRNTGLTPKTEIAGNTEVQGYLGKYLDVGEYVFDSSVGIMNASGYFVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAL8</accession>
    <name>A0A1E3PAL8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_60401</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041547.1">
        <property type="nucleotide sequence ID" value="XM_019182984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAL8"/>
    <dbReference type="STRING" id="683960.A0A1E3PAL8"/>
    <dbReference type="GeneID" id="30200230"/>
    <dbReference type="OrthoDB" id="629492at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF523">
        <property type="entry name" value="STRESS-INDUCED-PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="73"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="222"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="188"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="153"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="206"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="701" mass="81158" checksum="B0429DD7A0A27292" modified="2017-01-18" version="1">MSSEDVVATALEVGNKQFKLQKYDECVKIYSKAIELVESLSQKTILKLRKSYGLTERPAYLKQGENVNHPKLLTLLDNRAAAYEKLQNYQQAVQDSQRAVSIEPFNAKGYIRMGKLMLFMKKETKAYEVFSKGVKRIEYGVTKYGIKVNPNLLQHLKAQKQQLTSKLNEQKAQVKESERYPDIKRASESLVRSVSGSSQERKRAKTSSSLNPKGSSQLSRSFDLLLWLPIEIITHIFSHLPLKDVLNCLLVSPEWNHTLSRIPALFNNISISRFASLKQVQSCFSLLLKSRRSSHIKALDHFRLHSIRRTDERQILNFILNKSSICFTGDVDFSFLDVTTEQLVDCLKRNSVALRKLETLKHLKLSCVLVPTAEESLLELLPNLESFELIRVPDNKRPSTLRRYQANDHTFPNLKKLTLVGDIKSKYPSIPFFDHFLNQVQSFPNLKSLTIVGYDFQTLNTASREFNFLQNLPKLQSLVFENNDNFNLSTFFKSHDLLEFKELKKFALREREIRYSEHLMHYDSFYLSRIFQNLRILDLTGSSITWQGLSKILKVCGSSLQQLSIGYCQNVIFKKGPFRVHVNGGFFDFENFFKWCPNLQVLYLNQSTDFNDYSLTQMVTALKQQELFKNLKLLDLSFNEVSGYKLLDLVKVLKIESLILHGLDIHTETIKLMESSYCKKVESRIDKQSWREYGINSYNPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAN6</accession>
    <name>A0A1E3PAN6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_76653</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041693.1">
        <property type="nucleotide sequence ID" value="XM_019184426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAN6"/>
    <dbReference type="STRING" id="683960.A0A1E3PAN6"/>
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    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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    <dbReference type="PANTHER" id="PTHR47793">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT CTI6"/>
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    </dbReference>
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    <dbReference type="SUPFAM" id="SSF57903">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ZF_PHD_2"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="42"/>
            <end position="92"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
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            <dbReference type="Proteomes" id="UP000094112"/>
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    </evidence>
    <sequence length="428" mass="49280" checksum="167738E26386419E" modified="2017-01-18" version="1">MPSLRSTRSADKTNPRQEQEEEEEEEEQYDEELDEEDDEEAETRCICGQADPPDENGLFIQCEKCSVWQHGYCVSISDIVPEKYWCEICKPELHTIIIRPDRKMTKYLPVQPKETSSALNRRKNRRAASAGTTSSNGKDHQSKEIKKEDLDEAAQRKRRDRGAYSSREDARYEALIQRVMEESKKDASTTSEQEDEKSQSRSSRSSRRRQTRKDDVDGEEEENEENDENDKEPHDEEDDGVKKEFEENHEDEKKNSSKRKRSEEAPDSAGNANIQQNGPTDNNGSRPSSTSKAGKVSGESESNTESTSTKAKKRPKKAKTNHKSPQKDKDSTIDFNKPTKPRLPNQRTTMNEMRKRVAAILEFIGRTQVDIATEQDDKSELTKYVEDDVHKEKIEKLFENYNGSLELMDGLTRKLLIWEEKYGKLGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAQ1</accession>
    <name>A0A1E3PAQ1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription and mRNA export factor SUS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SUS1</name>
        <name evidence="3" type="ORF">WICANDRAFT_60552</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in mRNA export coupled transcription activation by association with both the TREX-2 and the SAGA complexes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction and promoter selectivity, interaction with transcription activators, and chromatin modification through histone acetylation and deubiquitination. Within the SAGA complex, participates to a subcomplex required for deubiquitination of H2B and for the maintenance of steady-state H3 methylation levels. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery. May also be involved in cytoplasmic mRNA decay by interaction with components of P-bodies.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the nuclear pore complex (NPC)-associated TREX-2 complex (transcription and export complex 2), composed of at least SUS1, SAC3, THP1, SEM1, and CDC31. TREX-2 contains 2 SUS1 chains. The TREX-2 complex interacts with the nucleoporin NUP1. Component of the 1.8 MDa SAGA transcription coactivator-HAT complex. SAGA is built of 5 distinct domains with specialized functions. Within the SAGA complex, SUS1, SGF11, SGF73 and UBP8 form an additional subcomplex of SAGA called the DUB module (deubiquitination module). Interacts directly with THP1, SAC3, SGF11, and with the RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ENY2 family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ62496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183014.1"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071819">
        <property type="term" value="C:DUBm complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.140">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ENY2_Sus1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TF_ENY2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038212">
        <property type="entry name" value="TF_EnY2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12514">
        <property type="entry name" value="ENHANCER OF YELLOW 2 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10163">
        <property type="entry name" value="EnY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 2" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="97" mass="11164" checksum="A91B05C43FB9E16B" modified="2017-01-18" version="1">MANETDLLKAKIQERLIESGEYDRISTALKQKLAESGWYDNIKSIANDQISHEDSLNFSRLNQQLEPKALEMVPESVKNDTLLQIKQFLETVVEKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAX2</accession>
    <name>A0A1E3PAX2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_76686</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ62518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="151"/>
        </location>
    </feature>
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            <end position="306"/>
        </location>
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            <end position="451"/>
        </location>
    </feature>
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            <end position="618"/>
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    </feature>
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            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
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        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
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            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
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            <end position="87"/>
        </location>
    </feature>
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            <end position="98"/>
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        </location>
    </feature>
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            <end position="441"/>
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    </feature>
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        <location>
            <begin position="517"/>
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        </location>
    </feature>
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        <location>
            <begin position="543"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="618" mass="69571" checksum="795333A16F034967" modified="2017-01-18" version="1">MRSFIKAHRRGSSTNINGEVPLELNTNSGTSTSISADNIPQLQAQQPDEKLQNGSISANSSAQSSPIRQSPSSPIVNKGKKLHRLFQRNKTSSPNLINLASPLSPKKSPPPSIIGTRKHEWGNGSSDSHKSFNKVSSNRQSTQSSQSSTANSLYSLEDQNFLSSTSTQNKDALQTNTNDDNDFLQLPLNTVLEIPNESSLSPFMREIPDSADMDKKSRHRRAQIISADSFKLEDPPEIIPAHELQKVKEVSSPELFNDGFVDPDETVAETIRNQKQRNNIVQGEQDKHDEFSSSSDGDSEFSFEQDNKRGRNVSIKYYKSPEERFREEEARVVKEQGFYVNDLIDDNFDDDMNYYDEYDDEFNDDEEIFNKKYFSDDEDKEIVPKFKTQPSVKDDKITPKNSISSKLTTASSILSSNKDITPSNTLTTHRNGLINQSNSQIKPKPSSLKEDLLKPSRSLKYHQISTTLDQDVPQNRYSWFSDDEDTPHRAVNSSYDSLLDEINNIPEDFEFGHKADNPQNQQQPRSKSMNSSIKRSKGVFEDNPQNTKFESNNKTVTLFNRPSRQNSVKSNDSNDEQQQMSFGYMTPNGSFTVPNPGFANSQNIELSPITEGSIDGSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB99</accession>
    <name>A0A1E3PB99_WICAA</name>
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        <recommendedName>
            <fullName evidence="4">Peroxisomal targeting signal receptor</fullName>
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        <alternativeName>
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        </alternativeName>
    </protein>
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        <name evidence="17" type="ORF">WICANDRAFT_26809</name>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
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    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxisomal targeting signal receptor family.</text>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PEX5/PEX5L"/>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
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        <property type="entry name" value="GH08708P"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR PEX5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
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    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0882">Thioester bond</keyword>
    <keyword evidence="9 15" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="repeat" description="TPR" evidence="15">
        <location>
            <begin position="323"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="15">
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            <begin position="433"/>
            <end position="466"/>
        </location>
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            <end position="500"/>
        </location>
    </feature>
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    </feature>
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        <location>
            <begin position="83"/>
            <end position="144"/>
        </location>
    </feature>
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        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
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            <end position="126"/>
        </location>
    </feature>
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        <location>
            <begin position="127"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005348"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00014710"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00032505"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ62570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="588" mass="66564" checksum="7FEC367FB5890640" modified="2017-01-18" version="1">MSLFGSGAECSTARNPLSQFTKHTNEDRSLQRPGLNQQINNQGGIREETRMNQHDQQRMNQFFKGQDSFNFQPMNHELAHIHTHQQPQGFRQSPSSNWSSEFKTDSPLQHNGSLRSNGSRWSSEFNTSTASPMSSTTTLSEPQSHANPMVRQRIGGFNPVFSSYHNGMVNHVQPVHTNSKIAEVGNNEQWEDRFKEIDEQFQDAFSKQEGKQNEEELKDQEKNVDPEIIIDDKYQTNFDEVWESLNSQTVQDDLQAEPSWEKDFATYAQARANFGKYQFEQTNQFLNNPSAYEIGVTLMEQGAKLSEAALAFEAAIQENPRHTDAWLKLGEVQTQNEKEIAGLTALEKCLSLDPNNLSALMTLAVSYINEGYDNAAFATLERWIETKYPQVTDKARSTNPKINDEDRFSLNKRVTELFIQAAQLSPAGANIDADVQMGLGVLFYANEEFEKTIDCFKAALAVRPNDELLWNRLGASLANSNRSEEAIEAYQRALQLKPSFVRARYNLGVSSINIGCYKEAAEHLLGALSMHKVEGLDDDDLRNNAIANQSTSILETLKRAFVAMNRRDLIEKVKPGMNLNQFRGEFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBD4</accession>
    <name>A0A1E3PBD4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF300-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_82662</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041895.1">
        <property type="nucleotide sequence ID" value="XM_019185418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBD4"/>
    <dbReference type="STRING" id="683960.A0A1E3PBD4"/>
    <dbReference type="GeneID" id="30202664"/>
    <dbReference type="OrthoDB" id="5348404at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044395">
        <property type="term" value="P:protein targeting to vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="488" mass="55850" checksum="304EA149388B1D42" modified="2017-01-18" version="1">MVEPLLPHVTILVCGIGSLVSIIISTIGIYLQFKNYRKPYEQRLIVRIQLMVPLYAVTCFISLRWFPVSKFVEPIREIYEAFVIYTFFSLLTHLLGGERKIVVMTSGRQPIKHPWPFSTVLPDIDISDPFTLLSIKRGILQYVWLKPIICILTAITETFGLYNSESNSFFNPYFIINFIYNVSVSVSLYDLALFWKCLYGDLRPFNPWGKFLCVKLIIFASYWQGVFLAILSWFGILKNDQSDSNNTLGFAIQNALMCIELIGFAIGHWHSFSYTEYDMKTLPGCARFEILAAIRDTFGIGDLVYDFKATLSGESYDYRQFDSVEALIAHPSSRSRMARFNEGLRYTDSGKQKYWLPNTKNGTTVGGMSAHLKTPLLSKNRSNSITSSHASIRGLYPNSVLTVDSKNEEEHPVASIYNYNTIEETDEDQDNEFLEDEQLFADAKANCPFGDKNYPVVYDKEAYTYSPALQRLREEAMARQSIESTRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBM1</accession>
    <name>A0A1E3PBM1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_25544</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041821.1">
        <property type="nucleotide sequence ID" value="XM_019181157.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3PBM1"/>
    <dbReference type="GeneID" id="30198403"/>
    <dbReference type="OrthoDB" id="1029639at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035621">
        <property type="term" value="P:ER to Golgi ceramide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120010">
        <property type="term" value="P:intermembrane phospholipid transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055091">
        <property type="term" value="P:phospholipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060304">
        <property type="term" value="P:regulation of phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04044">
        <property type="entry name" value="C2A_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04052">
        <property type="entry name" value="C2B_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04045">
        <property type="entry name" value="C2C_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04040">
        <property type="entry name" value="C2D_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21678">
        <property type="entry name" value="SMP_TCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037761">
        <property type="entry name" value="C2A_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037765">
        <property type="entry name" value="C2B_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037762">
        <property type="entry name" value="C2C_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037756">
        <property type="entry name" value="C2D_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056910">
        <property type="entry name" value="TCB1-3_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017147">
        <property type="entry name" value="Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052455">
        <property type="entry name" value="Tricalbin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980">
        <property type="entry name" value="TRICALBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980:SF1">
        <property type="entry name" value="TRICALBIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24920">
        <property type="entry name" value="C2_TCB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25669">
        <property type="entry name" value="SMP_MUG190-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037232">
        <property type="entry name" value="Tricalbin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="14">
        <location>
            <begin position="113"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="311"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="460"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="596"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="924"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1124"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1300"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1131"/>
            <end position="1157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ62614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1326" mass="147345" checksum="39714BC6841D02BC" modified="2017-01-18" version="1">MPKEPRKQERFPWEKIGGFQEKGKFDPHFVTDSQIVKTYIIETFYSDWYWSTGLVIGTCFFSWLIAKWRFGIFGLMFVLLGTSSVYRAEFRRFSRNIRDDLQRNAAAEKLGENFETMEWLNSFLAKFWVIYMPALSETVMTIANDTLKDIAPGYGIDALSLDEFTLGSKAPRIDSIKSYTKKGKDVVEMDWAFSFTPDDTSDMTKREIEKKINPKVALGVRVGKAFVSKKLPILVENMSVSGRVKITLNLSLNFPHIKIVSISLLEAPKIDFSLKPIGGDMLGLDIMSLIPGLSTLITTLINSNVGPMLYAPNHLDIDVEELMAAQANDAIGVVAVTVKGANDLKRSSHHGFNPYVQLFVDNEPEKYVRTDVKANTKTPRWNETKYILVNSLEQKLNLDLHSFSTEDKKGSLFASHTIELADLLQQEAIIDSNKPLELAGKKKGYINYDIRWFPVIESEKSSTEDESKEVETPDTEVGIFKLTVHQAKKLDATASLIGQLNPKAELYVNGKSTKTFRTLKQANEPAWEETLEILVTQKSNTKIKLVISDTGSSDEDATIATLEESLDTLVFNTIDGNDSFPLSPKGEVRLTAQWKPVSLSGVSAAANFVPPIGVARLHLRKGIDLLNLEKIGKVDPYVKVMLNNRLKYQTVFYPDTLDPNFDEVVYLPITSQSQHVSFEVMDEQKMSKDRSLGSCDFPIGNFVSKDANDNFLFYDGANKILKSKLSLKGKTAHGELTYSVSFLPAIPVYSLTELEQLEQEREKKAAREKKYQAQLKDWEEVYKKNPKDYEWVDVEDQDEFDEDAKEKLSLEQLLTYRSGTLGINILKGKVKKADTFIQVLIDDSGSPSFVSGRATARNVNPESGEAFIRDLPNSKIVFRNTKKQQVKDSGDVLGEQIYNTIELLEKGYDEPYHVTIDGASVEVRFEYIPSAIKLPPSETILDTGRAKIEFLDAEGLMSADRNGKSDPFVSLTLNGLELFKTKVVKKTLSPTWNEGTTIPVPSRARSDLMMTVYDWDRAGKNDLLGQARLDITGVKPLTSDIIHIPLDTQGSVRLRVTFVPEYIRPKIGAAEFGVNLSSIAGAPLKTVGAAADLATGVVGGGVGVAAGAVGKGGSLFKNVLTGKKSKKSLDQPRSSMDTGSQYANDVQSMRSGQTKSTTSKDKEGGAIAAAQEENGDDQASTYTTQVKDKKTTKGKLIIRSGTNLGNHAQVKVSLAINEKLREIYHTKNVKATNHVLRWDEETSFDAPMDAEVVFGGLLHHKFSKDEELGTASIKLAEVVDSPRDIDLKLGQGQIVVSFRYSPEPEEGQEDGQTPPPPGWHHEDLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBQ2</accession>
    <name>A0A1E3PBQ2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RecQ mediated genome instability protein 1 OB-fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_60895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042056.1">
        <property type="nucleotide sequence ID" value="XM_019183078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBQ2"/>
    <dbReference type="STRING" id="683960.A0A1E3PBQ2"/>
    <dbReference type="GeneID" id="30200324"/>
    <dbReference type="OrthoDB" id="341511at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.770">
        <property type="entry name" value="RecQ-mediated genome instability protein Rmi1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042470">
        <property type="entry name" value="RMI1_N_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013894">
        <property type="entry name" value="RMI1_OB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08585">
        <property type="entry name" value="RMI1_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecQ mediated genome instability protein 1 OB-fold" evidence="1">
        <location>
            <begin position="42"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="219" mass="24474" checksum="7F3202DF36183E13" modified="2017-01-18" version="1">MTQLIRYLIADLANTSPNNLEFASDEKSQRAFKASKISIDTNSNRIVKETLFQIVTLENISQSKVSKLDELLAADDLDSVGADRLRGRQQRIIRNVNVEDQDNGGGGALPTSSNASNYFKLTLQDCFGNLIYGIEQEKLQFLSGKNFKSGFPIQLGSKVLIGNAKIVHGVVLLKPSNTEFLSGMVQMWNIDLPKKHINYLKNELEKIKAQNQDQDEVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBT3</accession>
    <name>A0A1E3PBT3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BRO1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_25575</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the palA/RIM20 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042086.1">
        <property type="nucleotide sequence ID" value="XM_019181159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBT3"/>
    <dbReference type="STRING" id="683960.A0A1E3PBT3"/>
    <dbReference type="GeneID" id="30198405"/>
    <dbReference type="OrthoDB" id="64867at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09241">
        <property type="entry name" value="BRO1_ScRim20-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08915">
        <property type="entry name" value="V_Alix_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.560">
        <property type="entry name" value="alix/aip1 in complex with the ypdl late domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.50">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025304">
        <property type="entry name" value="ALIX_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF39">
        <property type="entry name" value="PROGRAMMED CELL DEATH 6-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13949">
        <property type="entry name" value="ALIX_LYPXL_bnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="3">
        <location>
            <begin position="4"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="628"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="720" mass="83256" checksum="CB8F32F5DA4DEDF7" modified="2017-01-18" version="1">MSSNLLFVPYRKTKRIEFSKELRDIISKEYYQTATVFEADLQEIDTLRNDTIDLEVSPQDLALLKRYYIHLSTLESKFPRDVVEFPWYGTLGYNVTGPVKIRSFTFERINVLYNVASLYTQMAVNQDRSSDEGLKKACLYFQYAATFFGYINKVAEQDEHLTLPLDLQKGTIETLQLLSFAQAQEVFWLKATRDKVKDSLVSRLAVQVSDYYTRTLTNANRSEGFRTEWIHHITVKKHHFQAAAEFRASLVSVSNSKYGEEVARLYKAKSALGLANSSIRFASEALKQDLQGLNDVVTDTLRRAEKDNDLIYLQDVPKTLPDIVKASVVKEMEISDFTKPNESLKTGNYGKQLFKDLLPFYVIQTAQAFRERQDEYVQRHIIGPVKALTNIMNKYIVERGLPGSIDAFDKPQALPSSLLDHNSEIKSQGGVRLVEETIDDTQRLSLEGERLLEGAKDRLKVEAGEDELLRTRQGSKHWTRPTSEEAAAHLTKRIQDLQNYLRQAKKGDETIRNQYYNIETPLKILASSEEKIMEFVPNSKSQQLDPSLRKSILSIREVLNRAHKLEVERNNFIETVEVKSLQYNILPKIVSEYKSIQSNFNDLKVDSNTFEPVFQKHIQNFSKDIDWIEKQKTEQREVEDKIDELNKQFMRLKTSQGVSSSREEALKTLDLVYTEFKELKLNLSQGLQFYNDFNANCKKLIDDCDNFVYERRLEARDLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBY2</accession>
    <name>A0A1E3PBY2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Farnesyl pyrophosphate synthase</fullName>
            <ecNumber evidence="6">2.5.1.1</ecNumber>
            <ecNumber evidence="5">2.5.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">(2E,6E)-farnesyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Dimethylallyltranstransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Farnesyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Geranyltranstransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_27072</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Isoprenoid biosynthesis; farnesyl diphosphate biosynthesis; farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis; geranyl diphosphate biosynthesis; geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.1" evidence="6"/>
    <dbReference type="EC" id="2.5.1.10" evidence="5"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041679.1">
        <property type="nucleotide sequence ID" value="XM_019181334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBY2"/>
    <dbReference type="STRING" id="683960.A0A1E3PBY2"/>
    <dbReference type="GeneID" id="30198580"/>
    <dbReference type="OrthoDB" id="10257492at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004337">
        <property type="term" value="F:(2E,6E)-farnesyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004161">
        <property type="term" value="F:dimethylallyltranstransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:geranylgeranyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045337">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000006">
        <property type="entry name" value="Farnesyl pyrophosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039702">
        <property type="entry name" value="FPS1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11525:SF0">
        <property type="entry name" value="FARNESYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11525">
        <property type="entry name" value="FARNESYL-PYROPHOSPHATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01017">
        <property type="entry name" value="Polyprenyl_Transferase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ62472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="352" mass="40509" checksum="C58BB7A9CEF506AB" modified="2017-01-18" version="1">MSDKVYKRDKFLNVFPELVEELLQVLKDYGMSQEAIEWYKGNLNYNTPGGKLNRGISVVDTYIVLKGLKSADDLSVEEYKKIAILGWCIELLQAYFLVADDMMDQSITRRGQPCWYRNENVGNIAINDAFMLEAAIYVLLKKHFKSESYYVDLLELFHDVTFQTELGQLLDLITAPEDHVDLSKFSLEKHSFIVIFKTAYYSFYLPVALAMFAAGINDPKDLKQASDVLIPLGEYFQIQDDYLDCFGKPEDIGKIGTDIQDNKCSWVVNTALKLVTPEQRALLDKHYGQKNDDSEKNVKELFHNLGIQQIYLDYEEKTASDLKEKIAKVDESRGFKGEVLTKFLEKIYKRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBY5</accession>
    <name>A0A1E3PBY5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cell wall mannoprotein PIR1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_27067</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PIR protein family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042130.1">
        <property type="nucleotide sequence ID" value="XM_019181332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBY5"/>
    <dbReference type="STRING" id="683960.A0A1E3PBY5"/>
    <dbReference type="GeneID" id="30198578"/>
    <dbReference type="OrthoDB" id="5415592at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054508">
        <property type="entry name" value="PIR1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051153">
        <property type="entry name" value="Yeast_CWMannoprotein_PIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000420">
        <property type="entry name" value="Yeast_PIR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254:SF1">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00399">
        <property type="entry name" value="PIR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22799">
        <property type="entry name" value="PIR1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00929">
        <property type="entry name" value="PIR_REPEAT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50256">
        <property type="entry name" value="PIR_REPEAT_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133773" description="Cell wall mannoprotein PIR1-like C-terminal domain-containing protein" evidence="9">
        <location>
            <begin position="20"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall mannoprotein PIR1-like C-terminal" evidence="10">
        <location>
            <begin position="276"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="87"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="223"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="229"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="359" mass="36773" checksum="6BCE78FC4A0AF306" modified="2017-01-18" version="1">MKYSYSALTAATLITSTLAGVVPHEPWTTLTPSGKAPKGATTDYTATFGVAVVPITGTTTSNGSVSTETGSATKMKRDVVSQIGDGQIQASTATPSTSAAPKSTAQAISQIGDGQIQATTNTEKPKSSEKPKSTAQAISQIGDGQIQATTNTEKPKSSEAPKSTAQAISQIGDGQIQATTNTEAPKSTAQAISQIGDGQIQASTNTEKPKSSATAVSQIGDGQIQATGSSNSSSPATKSSESLAKATESSKSSDDNVKAVSCKNPGSLALSLNGSVLTDSSGRIGSIVSNRQFQFDGPPPQAGAIYAAGWSITEEGVLALGDSDKFYQCLSGNFYNLYDENVADQCHEIKLEVIELIDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBZ0</accession>
    <name>A0A1E3PBZ0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_87914</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 10">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041604.1">
        <property type="nucleotide sequence ID" value="XM_019186131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBZ0"/>
    <dbReference type="STRING" id="683960.A0A1E3PBZ0"/>
    <dbReference type="GeneID" id="30203377"/>
    <dbReference type="OrthoDB" id="282270at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001193">
        <property type="term" value="P:maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10508">
        <property type="entry name" value="Zn-ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000016">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034012">
        <property type="entry name" value="Zn_ribbon_RPB9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 8 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="11">
        <location>
            <begin position="59"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="101" mass="11649" checksum="5BA5FBDE13384AB6" modified="2017-01-18" version="1">MLYPKEDKQANRLLFECRICEYVEEAATPLVYRHELISNFGETAGIVQDIGSDPTLPRSDKECPRCHAHENVFFQSQQRRADTSMVLFYVCLACSHTYKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCL8</accession>
    <name>A0A1E3PCL8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DUF726-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_82910</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMCO4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042237.1">
        <property type="nucleotide sequence ID" value="XM_019185463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCL8"/>
    <dbReference type="STRING" id="683960.A0A1E3PCL8"/>
    <dbReference type="GeneID" id="30202709"/>
    <dbReference type="OrthoDB" id="277931at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035650">
        <property type="term" value="F:AP-1 adaptor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035652">
        <property type="term" value="P:clathrin-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007941">
        <property type="entry name" value="DUF726"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029024">
        <property type="entry name" value="TerB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920:SF3">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4 TMCO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05277">
        <property type="entry name" value="DUF726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158682">
        <property type="entry name" value="TerB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="509"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="552"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="663"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="206"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="940"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="212"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="957"/>
            <end position="966"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ63030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1007" mass="112288" checksum="D1AF8D969E9E8683" modified="2017-01-18" version="1">MDQKDKDAIDCTDVDKFNKPTLSVPQLSYSSDSLGRINNRSQQQVNSKDTISPCHVGIIPSASASQVSILDVTRKSNDQQRGFYSHSDYVEFDLGHNDSKTGVVSSDRLVINRNDGTKAKLVKKGLAGDEISHEDKAAQLYLGETLNSVFKPIDDHTESPYSNITETKTDQLAGNSQRHKTIVEQNGVEEEDEDEWKQMEATANDDFYGENGASTTEADTNPEASHEGGRTGYTRIAAEEQAERYKEADKKTDFLFKSNKGPRSQVADAFYDDMDEEDNFEEDTFDYQTQNPLSQLQTTKKLLKDSQKFGYVGLVKLVMNEMATDLAKQALMSKNYFRGSKFVKRMNLSQSNFGQWQYKIIDRLYSHLELTKEESKMIESLSSHGIEPDDLAKSLKVHEIVENPAFDREAQKSVDSKSLEDKNATIEIDVAWTVLCDLFLVLLADSVYDSRSRTLLIKFGKLIGLDELEIFQFERRITDSLQLEDSSEQVWDESEILSSRKRKAKKKKLMYVGIATIGGSLVLGLSAGLLAPVIGAGIAAGLTTIGIGGTSGFLAGAGGTALVTVTGTAIGAKIGSSGMIKRVGDVKTFEFIPLHNNKRVNLIITVSGWMNSKADDIRLPFSTVDPVMGDLISLLWEPEMLQSTGQTVGILATEALTQSVQQILGATILMALMSAVQIPMALSKLGYLLDNPWNVSLDRAWKAGLILADTLISRNLGVRPVTLVGFSLGSRVIYSCLLELAKRGAYGLVEKVVLFGSPVVVSTEELALARSVVSGDYINGFSKKDWILGYLFRATSGGLRRVAGLSPIENFAGIQNFDCTSYVQGHMEYRKAMPQLLSQLGFQVLAEEFVEIDDPDPEQTERQRQLILEFDEARKQMENEQANGKKKGFWKKWFGPKKKEWWNIYNVDDLNKSDNSGKETPQKFDFDVDEVMNEVEQLKLNEEIHEKNRKTKPNQKGSEEESKSIEPEPLAIDHNFILYTKSKEDFSINLNSTSSIRNFEIVQNLRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUF8</accession>
    <name>A0A1E3NUF8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pyruvate dehydrogenase E1 component subunit beta</fullName>
            <ecNumber evidence="10">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_86259</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="10"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036026.1">
        <property type="nucleotide sequence ID" value="XM_019186023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUF8"/>
    <dbReference type="STRING" id="683960.A0A1E3NUF8"/>
    <dbReference type="GeneID" id="30203269"/>
    <dbReference type="OrthoDB" id="10266385at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000006">
        <property type="entry name" value="Pyruvate dehydrogenase E1 component subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027110">
        <property type="entry name" value="PDHB_mito-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006667">
        <property type="entry name" value="PRK09212.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008854">
        <property type="entry name" value="PRK11892.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624">
        <property type="entry name" value="DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624:SF96">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="9 10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="11">
        <location>
            <begin position="48"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ56819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="375" mass="40639" checksum="13BF0FFDE40F45EE" modified="2017-01-18" version="1">MVSKLSQVAQTAKLASSLVQRQNLAKAGLQSAFINNRLNSTKAEPTKLTVRDAINSALAEELDRDDEVFIMGEEVAQYQGAYKITRGLLDRFGEKRVVDTPITEMGFTGLAVGAALAGLKPVVEFMTFNFAMQSIDQIINSAAKTYYMSGGIQPCNITFRGPNGAAAGVAAQHSQDYSAWYGSIPGLKVVSPYSAEDHRGLVKAAIRDPNPTIILENEMLYGEAFEVSEEVLSPDFVLPIGKAKIEREGSDISIVTHTRNVGYALETAKTLQEEYGVSAEVINLRSIKPLDVPTIVESVKKTNHLITVEAGFPAFGVGSEICAQIMESEAFDYLDAPVERVTGAEVPTPYAKELEDFAFPDPPTILRAARKVLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV12</accession>
    <name>A0A1E3NV12_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_85943</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-14 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036185.1">
        <property type="nucleotide sequence ID" value="XM_019185972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV12"/>
    <dbReference type="STRING" id="683960.A0A1E3NV12"/>
    <dbReference type="GeneID" id="30203218"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01366">
        <property type="entry name" value="KISc_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000065">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972:SF45">
        <property type="entry name" value="PROTEIN CLARET SEGREGATIONAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 10 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="14">
        <location>
            <begin position="364"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="192"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="460"/>
            <end position="467"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ56978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="713" mass="81917" checksum="1C026905BFB0AEE3" modified="2017-01-18" version="1">MSSNRQSLIKQPSTLLPTKRTSMGINVAMNNNEGAVGEENKKNISSLPKPSGIPRPNTTPTKTSSYLTPYGYKSPSKSNHQERRKSLKEQIDIKEKDFTELKNSILSLKTSKQQLEFDNQVLEEKLSALNKDLKIKSNKIDNLASKKDEVQIDIKRSYELQLKQLKVENESCMYEIEKKYKNEIEKLLSLKVSEIQLQKTQLRDKISSLNNDIQNNEVNFQDNLNSLRKQYYTKQVELNKLLLETRITLEDECKDLDNKHKELEIELMETEKELQGETKAKQDQLKKLEETENSTRDAELHLKQLQETLETLEQSLKEKKFKIQDTQADIQWKLSEKQHFEQQLIHEEVMRRKLHNKMQELKGNIRVFCRIRPPLGPEIEDVVEVQVPDNDEEEQEIVLKDPKPISAGSFNGNSQTKKIYNFQFDRVFQMDSTNQEIFEEISQLVQSALDGFNVCIFAYGQTGSGKTFTMSNENDGMIPKAVEQIFQTAEKLSAQSGWEFKIFGEFLEIYNENINDLLGNPNNIDKLKLEIKHDTILQKTFVNDLTSVELENPKMVNEILSRALKNRSIAATKANERSSRSHSVFTIRIHGFNPNTNEKSEGVLNLIDLAGSERLSHSQASGDRLKETQSINKSLSCLGDVIYSLGCENSKHVPFRNSKLTYLLQYSLMGNSKTLMFVNISPFKQHFNETINSLRFATKVNSTKIGTAKKNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVD0</accession>
    <name>A0A1E3NVD0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_58672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036270.1">
        <property type="nucleotide sequence ID" value="XM_019182829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVD0"/>
    <dbReference type="STRING" id="683960.A0A1E3NVD0"/>
    <dbReference type="GeneID" id="30200075"/>
    <dbReference type="OrthoDB" id="10257948at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071444">
        <property type="term" value="P:cellular response to pheromone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02989">
        <property type="entry name" value="Phd_like_TxnDC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21148">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="30"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="156" mass="18092" checksum="0E342D8303A2E968" modified="2017-01-18" version="1">MKQAQKNVEDGYGNVETLINEESVLQRTTTIKHVVLHFFHENFQKCKVMDDKLKVMAEKHLSTKFIRVNVENAPFLVTRLKIKVLPAVLIYINGVESNRLVGFDKLNFNESGDFQIESLEKFLLDNGAIQRKATNYKRILKSKQYQDDDDESDLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVG1</accession>
    <name>A0A1E3NVG1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">L33</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">YL33</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_65449</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036396.1">
        <property type="nucleotide sequence ID" value="XM_019183912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVG1"/>
    <dbReference type="STRING" id="683960.A0A1E3NVG1"/>
    <dbReference type="GeneID" id="30201158"/>
    <dbReference type="OrthoDB" id="1688503at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000009">
        <property type="entry name" value="60S ribosomal protein L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_uL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005756">
        <property type="entry name" value="Ribosomal_uL24_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="Ribosomal_uL24_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01080">
        <property type="entry name" value="rplX_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11143">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L26 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16906">
        <property type="entry name" value="Ribosomal_L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="KOW" evidence="6">
        <location>
            <begin position="54"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00080137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00082602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="127" mass="14085" checksum="2FFB9E9A5E340587" modified="2017-01-18" version="1">MAKQSLDVSSSRSKARKAYFTAPSSVRRSLLSAPLSKELREQYGIKALPIRKEDQVLVVRGSKKGQEGKISSVYRLKFAVQVDKLTKEKSNGATIPINIHPSKVVITKLHLDKDRKALITRKGGKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVJ9</accession>
    <name>A0A1E3NVJ9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Anaphase-promoting complex subunit 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_36554</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RING-box family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036363.1">
        <property type="nucleotide sequence ID" value="XM_019182445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVJ9"/>
    <dbReference type="STRING" id="683960.A0A1E3NVJ9"/>
    <dbReference type="GeneID" id="30199691"/>
    <dbReference type="OrthoDB" id="1681166at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16456">
        <property type="entry name" value="RING-H2_APC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000111">
        <property type="entry name" value="Anaphase-promoting complex subunit 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051031">
        <property type="entry name" value="RING-box_E3_Ubiquitin_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024991">
        <property type="entry name" value="RING-H2_APC11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11210">
        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12861">
        <property type="entry name" value="zf-ANAPC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="35"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ57156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="106" mass="12025" checksum="4F9259474108F950" modified="2017-01-18" version="1">MKVNIKNWKGVAVWSWDVPNEEVCGICRVSFDATCSACKYPGDECPLVVGVCTHRFHLHCILRWLDTDTAKSLCPMCRRAFKIKENYQQSQVDYGDPTPAHPASSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVS1</accession>
    <name>A0A1E3NVS1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_35934</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036516.1">
        <property type="nucleotide sequence ID" value="XM_019182375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVS1"/>
    <dbReference type="STRING" id="683960.A0A1E3NVS1"/>
    <dbReference type="GeneID" id="30199621"/>
    <dbReference type="OrthoDB" id="3941538at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003939">
        <property type="term" value="F:L-iditol 2-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006062">
        <property type="term" value="P:sorbitol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05285">
        <property type="entry name" value="sorbitol_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000068">
        <property type="entry name" value="Sorbitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045306">
        <property type="entry name" value="SDH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161:SF9">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="8">
        <location>
            <begin position="12"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="353" mass="37991" checksum="247F70EC19BDA049" modified="2017-01-18" version="1">MSNPSIVLKDKGQIVLENRPVPEIRDDHYVKIAIKKTGICGSDVHYYLHGRCGTFVVNAPMVLGHESSGVIVEVGKAVTRVKVGDNVAIEPGIPDRFGDEFKTGHYNLCPHMAFAATPPYDGTLARYYLMPEDFVVKLPDHVSLDEGALVEPLSVAVHAAKLGGIKFNSKVAVFGAGPVGLLAAGAARALGASEVLVVDIFDTKLELAKKIGATHVYNSLKKGDFVTEVLKQLGGRPDVILEASGAEIAIQNGLALVKTGGSFVQVGMGKDFVSFPIAQMTERELTVKGNFRYCHGDYQDAVDMISSGKINVKQIITHRFKFKDAKAAYDNIVKNGKDVIKTIIDGPEDTSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVT3</accession>
    <name>A0A1E3NVT3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_81443</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036431.1">
        <property type="nucleotide sequence ID" value="XM_019185234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVT3"/>
    <dbReference type="STRING" id="683960.A0A1E3NVT3"/>
    <dbReference type="GeneID" id="30202480"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000006">
        <property type="entry name" value="General amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004762">
        <property type="entry name" value="Amino_acid_permease_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00913">
        <property type="entry name" value="2A0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF9">
        <property type="entry name" value="DICARBOXYLIC AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="388"/>
            <end position="405"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="417"/>
            <end position="440"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="460"/>
            <end position="478"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="490"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="52"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57224.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
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    </evidence>
    <sequence length="570" mass="63190" checksum="9CA98EC3B2E754F7" modified="2017-01-18" version="1">MSSISEKKTDLYKDQPIDVEVGQIDTSSTHDDTNILAIKEGYRLKKHLRARHVSMIAIGGALGTGLLIGSGAALSTSGPAPLFIGYSFIGFVVYIVMCCLGEMAAYIPQPDGFAGYANRYCDPALGFAVGYSYLFKYLIATPNQITAGAMVLQYWVSRDKVNPGVWITIFIIAILLINIFGVKIFGEFEFWLSCVKVLIVLGLIILLFIIILGGGPTHDRLGFRYWSDPGAFKPYTGIASIPKGKFVSFVNVLVTAVFAYGGTELVGVTVAEAENPRRNVPKAIRLTFYRILVFYICSVLLLGMCVPYNDPLLISATKAKTSANASPFVVAIKNAHIKGLDHLINVCVLVFIFSASNSDLYIGSRTLYGLACNGIAPKCFAKTNRFGIPYYSLALCVSFCGLAYMSVSASSQEVFSYLVNVSTLFGLLTWATIIITYLYFSRAFKAQGISKDTLVYQAPFQSWAPHLALAICILVSFIKNFTVFINGFNYKTFITGYIALPVYVFCYLGYKIIKKSKIHRPEDVDLYTLKDLIDAEEEEGKIADALRKEEMKYGKKDKMWYYDKFIGWLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW04</accession>
    <name>A0A1E3NW04_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">WICANDRAFT_36757</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 25 26">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_019182469.1"/>
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    <dbReference type="OrthoDB" id="10248617at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:termination of RNA polymerase III transcription"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007175">
        <property type="entry name" value="PRK09606.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
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    </dbReference>
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        <property type="entry name" value="RNA_pol_Rpb2_2"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA_pol_Rpb2_6"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
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    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="19">
        <location>
            <begin position="42"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="18">
        <location>
            <begin position="188"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="20">
        <location>
            <begin position="443"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="21">
        <location>
            <begin position="541"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="22">
        <location>
            <begin position="623"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="16">
        <location>
            <begin position="670"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="17">
        <location>
            <begin position="1041"/>
            <end position="1125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="ODQ56872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1130" mass="126685" checksum="017B87798B71B9D6" modified="2017-01-18" version="1">MGTDDSFNELLKPVYKGKQLSDEINTAEDKWNLLPAFLKVKGLVKQHLDSFNYFVDVDLKKIIKANEKILSDVDPEFYLKYLDIRVGHKSTSSTKDPILPPHECRLRDLTYSAPIYVDVEYTRGRKIIMHKDLEIGKMPIMLRSNKCTLDGASEAKMASLNECPLDPGGYFIVNGTEKVILVQEQLSKNRIIVEADEKKQIVQASVTSSTHERKSKTYVVTKNDKIYLKHNSISEEVPIVVVLKAAGIISDLEIMQLVAGDDSTYQDLFVINFEECAKLGIYTQQQALEFIGSKVKTMRRAKLSVLQEGIEAIATTIIAHLTVSDLDFREKALYIALMTRRVIMAMHNPKMVDDRDYVGNKRLELAGQLMSLLFEDLFKKFNTDFKANIDKILKKPNRATEFDALLSINIHSNNITTGLNRAISTGNWSLKRFKMERAGVTHVLSRLSYISALGMMTRISSQFEKSRKVSGPRALQPSQFGMLCTSDTPEGEACGLVKNLALMTHITTDDEEEPIKKLCYLIGVEDISVIDSASLHSNFGVYLNGTIIGTTRFPTKFVSLFRNLRRSGKVSEFISIYTNLHQKAVHIATDGGRICRPLIIVENGESKVKAHHLLRLKQGKSQFDDFLKQGLVEYLDVNEENDCFIALYENDLSEQITHLEIEPFTVLGAVAGLIPYPHHNQSPRNTYQCAMGKQAIGAIAYNQFQRIDTLLYLMVYPQQPMVKTKTIELIDYDKLPAGQNATVAVMSYSGYDIEDALVLNKSSIDRGFGRCQVLRKNTTVLKKYPNHTKDIVTGMRVNDDGEPIFQHASLGPDGLGEVGTRVESGQVYINKCVPTNATDSIMNHADSEHRETPVFYKAPEPSYIDQVMMSVSDNDQALIKVLLRQTRRPELGDKFSSRHGQKGVCGIIVQQEDLPFNDDGISPDIIMNPHGFPSRMTVGKMIELISGKAGVLNGTLEYGTCFGGSKLEDMSKILIDKGFNYSGKDMLYSGITGECLQAYIFFGPIYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCVIAYGASQLLLERLMLSSDAFDVDVCDKCGLMGYNGWCTSCKSAEHVIRMTIPYAAKLLFQELLSMNIAPRLKLGDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW17</accession>
    <name>A0A1E3NW17_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1989 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_65583</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036533.1">
        <property type="nucleotide sequence ID" value="XM_019183942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW17"/>
    <dbReference type="STRING" id="683960.A0A1E3NW17"/>
    <dbReference type="GeneID" id="30201188"/>
    <dbReference type="OrthoDB" id="504708at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018959">
        <property type="entry name" value="DUF1989"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31527">
        <property type="entry name" value="RE64534P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31527:SF0">
        <property type="entry name" value="RE64534P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09347">
        <property type="entry name" value="DUF1989"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1989" evidence="1">
        <location>
            <begin position="46"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="290" mass="33100" checksum="187DC67ABC50B681" modified="2017-01-18" version="1">MSKPAYPHSENPTPAYLPTKGSILDVDKEFYDSISNTKARELTYKEIVPIRSGKAWKVKAGSIVRITTPEGPQVCDFNCWNADNFRERFWASRTRQLQRSHVGKYDRLWSNLPYLRPLLTITGDSIRDRLNIQKIHDLLGTRCDPYVDKVLSGEDNDFHCHSNLYRSIIPYGGTEYDVHDVLNIFQVTGLDKEGRYYMETCPAVSGDYFEFFAEVDLLCAISACPGGDLSAWDWGEGDEDTKVDMVDCCRPLGIEVYKITDDAVLKNWKSPEVANYKGQHGLKFPTFQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW22</accession>
    <name>A0A1E3NW22_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="8">PPIase</shortName>
            <ecNumber evidence="8">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_81610</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="8"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035984.1">
        <property type="nucleotide sequence ID" value="XM_019185270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW22"/>
    <dbReference type="STRING" id="683960.A0A1E3NW22"/>
    <dbReference type="GeneID" id="30202516"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000032">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF327">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="9">
        <location>
            <begin position="28"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ56777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="188" mass="20468" checksum="109DD539614BFF96" modified="2017-01-18" version="1">MLARTFLKAPQGARLFSSSAAAFGTKVFFTPAIDGRKLDRIEFELYDDVVPKTAENFRALATGEKGFGFAGSPFHRIIPQFMLQGGDITQGNGYGGKSIYGDKFADEDFSKRHTKPGLLSMANAGPNTNGSQFFITTVPCPWLDGKHVVFGEVTKGYDTVKTIEAYGTPTGRPRAKITIEESGEIKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWA4</accession>
    <name>A0A1E3NWA4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_64797</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036658.1">
        <property type="nucleotide sequence ID" value="XM_019183806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWA4"/>
    <dbReference type="STRING" id="683960.A0A1E3NWA4"/>
    <dbReference type="GeneID" id="30201052"/>
    <dbReference type="OrthoDB" id="498590at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039662">
        <property type="entry name" value="Cohesin_Scc3/SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048610">
        <property type="entry name" value="SCC3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020839">
        <property type="entry name" value="SCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013721">
        <property type="entry name" value="STAG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199:SF0">
        <property type="entry name" value="LD34181P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199">
        <property type="entry name" value="STROMAL ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21767">
        <property type="entry name" value="SCC3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21581">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08514">
        <property type="entry name" value="STAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51425">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCD" evidence="2">
        <location>
            <begin position="323"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="995"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1004"/>
            <end position="1017"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1017" mass="116721" checksum="6242FC74AA49F466" modified="2017-01-18" version="1">MPTTASSTPVRRSQRIKEIVSSQPVVDDETIEGDGYEDEDEDEDEDEDETPRKRRKTGPAGKAIKEAPSKKGRKSAADTFNELKENFEENYIFQALNDAEVSISELANDWVQTYKAHKDLAKKDLINLILNAVGCFTKIEEHDVSNNESASETVSEIQTFFKRQKNHEFYLASKKPAYKHLKKNFINFMNEIISISDENGLLYENIVISEEDDEDVEEPEDSNLMEDLLIWLSSFSVSYIRALRYISTLSLLNIETTLCRIIVKVNSSLEKFKHQLEVEQKKATSTSKAAKKRIAQIESNVTQYSNQSLILENTIQDILNTTFIHRFKDIDYQIRVEAMGALGEWMDTYPELFFKVTYLKYLGWVLSDENASVRSQVIKTLIKLLKKNIIVTGLRQFFERFKTRVIEIAFHDVDHHVRTSAINLLIEINKVGFLEDEEIGKVSSLIFSSKDSKIIQLVSKFIQTVESENTKATLEALSVNIKSSEKLFPINLRDLIKIHNLIEILTVANEVYSENQPKQSLVKSNDLKPSLFAIAGKELFALSAYSEYWEFLIRYYLLDISSIEVADDELIKAIQLTAEQETILFGIIHGSLIHLKQVEDELKKKKKDDVDDASSQRLISHLPLILKKVENNDTNLSTFIQILSIYNIEFFENFNQANVYKSIFQELTKHFKNNSIETLKNEYIHLFKILSDSSGSTTIGEINVLFKDTITELIIEFENLLKDSDFELSSSTIRTIHEDYSFKLLVLGLQYDISEVFKTFEMVKDKIFTFLPDFEIEDEDVEPLSSLLKIYLSSTSWKLNFLLHTNEPLNVETELSQIPVFIQEIQYLLTKTNVIKFQTEVASVLIDLSTLLKNFFNEPNTNLTNLNRFYDIDLSSLSLSQDTIDSLLYLFLYKEAIFANLNEVHLDREDDEDVNLNSVKLDVSELDTEELTSVNWDAEKDMVVYGVKLLGLYKVGILSSKIVKRIELNKEELGDLFKSVFEGVNINDSTPVASAANITQSKEEEQDDEIPEIAMDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWV5</accession>
    <name>A0A1E3NWV5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_36214</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome b5 family. MAPR subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036264.1">
        <property type="nucleotide sequence ID" value="XM_019182409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWV5"/>
    <dbReference type="STRING" id="683960.A0A1E3NWV5"/>
    <dbReference type="GeneID" id="30199655"/>
    <dbReference type="OrthoDB" id="10257697at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF76">
        <property type="entry name" value="CALCUTTA CUP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="4">
        <location>
            <begin position="83"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="196" mass="22671" checksum="567BEC93625B5D6C" modified="2017-01-18" version="1">MSELRNRKNKKESSSTEEKKPFQNDDNSISFSFFDIIRMISGLLLLNLFLSYWFTGTFTYNYSGKLIDPHYLYFQAFGSYVNLTDSELAKYDGSIEGLPIYIGVNGTVFDVSSKKEIYGPDGSYYYLSGKDCARAYATNCLNQQTYDIRDLEPEEIRRLKGWHEFFETKYFKVGVVNHEPLTGPTPDREDCRGRGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX33</accession>
    <name>A0A1E3NX33_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_85836</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036948.1">
        <property type="nucleotide sequence ID" value="XM_019185954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX33"/>
    <dbReference type="GeneID" id="30203200"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="122" mass="14325" checksum="E29819C6F0DE4F8C" modified="2017-01-18" version="1">MVPINQRRNLNRELPESINIAVGRFLVIDNLEERKHDRFHLSFCKKLKDVANTDLNKVELVNLTDVLGLCGQLVVKNDFSKFNVKEDLTNLEQINNNDYVHYFMKQNTINWAKFAEAHQIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX81</accession>
    <name>A0A1E3NX81_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_81120</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
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        <property type="project" value="TreeGrafter"/>
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    </evidence>
    <sequence length="1143" mass="128438" checksum="1EB128BA6B5B82E6" modified="2017-01-18" version="1">MNTGTGNKERSPQDNQRPGATGGHKRSSVFGSSSNIFGHKPLTASQPSKTTNEIKNNAPSIQSREKPSSEDAKEKSDSKEAEHIITDSSNNKDTKEPNESTNTTTDPIKSEDKLVKEGSEIEELASGKSKPSTSFASYLSNVKKRKQEIEEKKIKELEETKANPTTSSNEDVTMTEDSVADVNIEENQQPEAKRQVSTESTSTEVKREDPTIQETPKDKTSDASKSPVSTLSSELSILPSSSSADKQDASRSFHIPPRIPESDSNSDEGDITRTPRRKQNTPGGELSVAEDSDAETVHGDSPPKPRRGRLVRKSEMGSDVSDKKTALEGNHQDLDSSSYHKKLILGKLERRSQSKSVSPQRHSSTAKRSKGKDQSGRAKLQRACDKGKLEEAKRLLEEGAEVNDQDNAGNSALHDAALNGHTKIVELLLQHNAHINLRSGPEVLDTPLIDAAANGHISTVKMLLKYGADPRIQNSHGQNALDSLEDSHPNTPELKVILREATLALKSKQIDVSGTASDLGDSSYDEDRSNSHSRNNPRSRKRGPRNDLLLVDFTTKEGREAVFSKASDGDVQFVGTYLENGGPPDSEALALAAKFGHTEVVNLFLAFGAKIDVTDEKGLTPLMQTVGRGHIDTVKLLLQAGADPAKLSRDNKSVLEIAKDSLISDEEEIRLLTDALNKVSNQSNPGAKSSNDTKVKKRKISLKDEQQDTHELKKKKHHSNQKQQHERSSAPATETMKERDHKVEKSRSHEVIKPKPVESKFSGPKSESMKRTHSSDAKDHVKVQKIDQPVESYEEKQARLIKEKEELRKVEEANELRAAKEKEDAKKRDEKEKEEAQKRKLKEIEDAKKRLEREREESKKREEFEAIRAAKRKAREQQFLNDFETAEKKKEEELKKLAELQAERKLKEAQENEARAKMEEEKKQIMSQKEEIERRRNIRSQYPYGLQKATFDGSRTKEEVLAYLPLYHFEKDGVQYCADLQVIMILGIEKFYDKYQELSNNKKSIEEKEKTPLFNYFYPFLGNFSFRSTSKQMENFNEELQKFTKLNINWINYEDVLKIIKQDFEPIYDVVITRTLAIDLNHGQDSTAAVTSNSTGFITPKVESEPVSRKVSSSYPSITKRLPLLLRNRPAVLKALSKNKKLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXA3</accession>
    <name>A0A1E3NXA3_WICAA</name>
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        <recommendedName>
            <fullName evidence="2">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">WICANDRAFT_65126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycotina</taxon>
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            <taxon>Phaffomycetales</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        </location>
    </feature>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="456" mass="51732" checksum="908FE1CEEB321A7E" modified="2017-01-18" version="1">MSAEPSDPPVTDNSEIASAAIAEKPEESTPALPTSDDQEPAPETEQSEPSQTDENAYGSFPPGTIVLGKLKSFPPWPGIVIPYELVPEGVNKSKPKHPTQPRPSKKKNQHSDKPIDTRLWCVRFLRDDTYMWGGVNEISLLTKEQIEKFLNDKKGKKLIKSAYEMALNPPDVEEFIIWGSNGKPIQIDNEINDADFEDEEGDGQSLEDDDGEEDEDEEDEDLEELKQPVKKSTKRGRAKNSKSPAKKQKTSTPKKTKGKPGRKPGKRKSPEPEPEPETSSDEDWDVDDETEPPVAENIPSAKQLSDDLKKRTPLIKKARVVLQDFFLESKEIETDVKSLKQVNTILDSLEKIPEVQLSLVKHYSLHRVMFDILKRPDLIDIKDVKKMRERISKLVEIWFDTVIEPTENWNFEEKVEEIEENNGELKEENGKEENSLQEEVKENGVKEEETSEPVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXC0</accession>
    <name>A0A1E3NXC0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_35786</name>
    </gene>
    <organism evidence="8 9">
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        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
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    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037013.1">
        <property type="nucleotide sequence ID" value="XM_019182354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXC0"/>
    <dbReference type="STRING" id="683960.A0A1E3NXC0"/>
    <dbReference type="GeneID" id="30199600"/>
    <dbReference type="OrthoDB" id="283111at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016480">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14134">
        <property type="entry name" value="PKc_CLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051175">
        <property type="entry name" value="CLK_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="183"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="560" mass="64799" checksum="7058FFDA2B54DB08" modified="2017-01-18" version="1">MEPGTRKRPRSSFSDWNNLLKKKFNTTTNNNNNDSNIHDDNTFNVNKDNSSVIILDSDDENYEFNDIDDDADENTSGIAVNGHNIYDNDDNESIVILKENQLPPNYTESANYNTGNNNFDYNYNDGTRSLDLQDQDKRRTNSLPQLPLSQISYRRVSELIKIVTDDDGYYIAEPGQYFANGRFKILSILGQGTFGKVIKALDLNSNETVAIKIIRAIPKYREASKIELRILTTLKNADPENLNNCIHLREVLDYENHICIITDLLDISLYEFLQNNKFKPFPGSQIQAITRQLIRSVAFLHDLKLIHTDLKPENILLTSSDFAKFKKNKVLKNPLINVIDFGSAIFNDEYHSELVSTRHYRAPEIVLGTGWSYPCDLWSLGCILVELVTGEALFKTHENLEHLAIMEKVLNCKIPNSMIKKIKNKEILNYFKKNNYHYHQYLLNYPNNETQEKSINFVKTIKKLDDLICSKLKIKIHINMNDSLQKNWDKNHSKNKSLNFETFQFWFYFIDLVKNLLKFDPNQRISAIDAMNHPWWDFGTIDEATSGLMNNDNSFGGKMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXI4</accession>
    <name>A0A1E3NXI4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_80036</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037075.1">
        <property type="nucleotide sequence ID" value="XM_019184990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXI4"/>
    <dbReference type="STRING" id="683960.A0A1E3NXI4"/>
    <dbReference type="GeneID" id="30202236"/>
    <dbReference type="OrthoDB" id="61110at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF6">
        <property type="entry name" value="SCF-ASSOCIATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="77"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="460"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="201"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="522" mass="57813" checksum="1994A8C3CCB80C97" modified="2017-01-18" version="1">MTLTFFDLGADIIESDIIPYLSPADIRSLKLASKSLYQLVDQSSFIWHELFKKTFGTKPTAFSMNKWPELYSIRAKGRFYSWGAMGGGRLGLSSKDVPTENLERQGFSSGVCKPTKVPGIEEILADVSAGGFSFQILTAHGEIYSSGSYHAGHQTGPGPDQSDYNEFQQQIRHQESVLFNPLNMSQRMNPVLPIRLGGVRHRPMNEEHPHPLPGSFPSSPYQIPQDDTPKPKSKVENRFLVKERAQFDTQFVSVSSGRAHFIAIDNTGNLWSWDRGDYGVKLKFVNQGGVDLKSQGRFVLKALAGWDSSVAYIYNYGLVYWKKREPLKKDQTEAKAHHVLIPNTGDINGPGKIVDFVAGEDFIIYVTLEGKIYRNDMIGEPSFPLIKFQDYLKFNGKSLAPKFVRLSGSFRSFTAFSNEDLVLLGSKNTDEPEIIDELQKVNCISVAVGDYHFLALLRDGTMLSWGLESNHCGCLGLGKQPNGSIIEGGSKRVIKPTQIDAEGKVVAIAAAGWQSAAIISDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXK2</accession>
    <name>A0A1E3NXK2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_65182</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endomembrane system</location>
            <topology evidence="6">Lipid-anchor</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037041.1">
        <property type="nucleotide sequence ID" value="XM_019183869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXK2"/>
    <dbReference type="STRING" id="683960.A0A1E3NXK2"/>
    <dbReference type="GeneID" id="30201115"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01861">
        <property type="entry name" value="Rab6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000970">
        <property type="entry name" value="Ras-related protein Rab-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="201" mass="23068" checksum="2F83C012B0F18BF5" modified="2017-01-18" version="1">MSKSNTLSKYKIVFLGEQGVGKTSLITRFMYDTFDDNYAATIGIDFLSKTMYLDDKTVRLQLWDTAGQERFRSLIPSYIRDSHVAVIVYDITSKKSFDLVDKWIEDVKIERNDNVVLCLVGNKNDLVDKRQVSIEDGEKKANFLNCAIFIETSTKLGYNVKNLFKKIAKSLPDFDETKDNQPEMIDISIDTKKEDEAQCQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXK4</accession>
    <name>A0A1E3NXK4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_80055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037095.1">
        <property type="nucleotide sequence ID" value="XM_019184996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXK4"/>
    <dbReference type="GeneID" id="30202242"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="255" mass="27465" checksum="3F66D56C9880CD9D" modified="2017-01-18" version="1">MASPFKKAKKLLKLEKFINKSSSKTSKVTKQSTPSESVTQNINTSSNNSNSDGKQQPEIAAPQISSNDAPSKIPSIPNPVSRATQTNSSPNPATTPEESKQNKPLTTKESIPSGNPSAAPKNVSSPTVPYSPRTYKESSNYLEKPNFPGSRFQTSPPPTSHDRLGAHPTTPYPSVTYPPTSQSSTTRSTEAFDPRQLIEIDLSNPDKPRLLIPKGEGITNEMISEIVCALQNEDDLSALVVIGNNTFIVCNAICL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXZ1</accession>
    <name>A0A1E3NXZ1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Coatomer subunit beta'</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_101395</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasmic vesicle</location>
            <location evidence="1 13">COPI-coated vesicle membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Golgi apparatus membrane</location>
            <topology evidence="13">Peripheral membrane protein</topology>
            <orientation evidence="13">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="13">The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the WD repeat COPB2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037268.1">
        <property type="nucleotide sequence ID" value="XM_019180320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXZ1"/>
    <dbReference type="STRING" id="683960.A0A1E3NXZ1"/>
    <dbReference type="GeneID" id="30197566"/>
    <dbReference type="OrthoDB" id="10261470at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22947">
        <property type="entry name" value="Coatomer_WDAD_beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000016">
        <property type="entry name" value="Coatomer alpha subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000001">
        <property type="entry name" value="Coatomer subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006692">
        <property type="entry name" value="Beta-prop_COPA/B_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016453">
        <property type="entry name" value="COPB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF2">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04053">
        <property type="entry name" value="B-prop_COPA_B_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005567">
        <property type="entry name" value="Coatomer_beta'_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 13" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <keyword evidence="5 14" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="9"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="93"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="136"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="180"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="224"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B second beta-propeller" evidence="16">
        <location>
            <begin position="318"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="17">
        <location>
            <begin position="593"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="789"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="802"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="833"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="844"/>
            <end position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ58061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="858" mass="97010" checksum="C24EADDD863BFE85" modified="2017-01-18" version="1">MKLDVKKQFSSRTDRVKGIDFHPTEPWVLTTLYSGRVEIWSYETGTKIKSIDVAEVPVRSGRFIARKNWIVVGSDDFQVRIYNYNTGEKVAQFEAHPDYIRSIAVHPTRPYVLTSSDDMTIKLWNWDNNWKLEQTFEGHNHYVMSLAFNPKDPNTFASACLDHTVKIWSLGNNVPNFTLNAHEQKGVNYVSYYPQSDKPYLITSSDDRTIKIWDYQTKSAVATLEGHSSNVSFAIFHQELPIIISGSEDATIKIWNSNTYKLEKTLNYGLDRAWCISSHKNSNSLAIGFDAGFAVLQLGNDEPRLSMDPVGKLIWAKNNEVYSSVIKGNEQVEDGETLPLSSKDLGSVEIFPTSLKHSPNGRFVTVTGDGEYIIYTALAWRNKEYGSALDFVWAQDPNIFAIRDAQNQIIIHKNFKPKPNGEIDFPYNVEKLYGGSLLGVKSDEFVAFYDWESSELVRRIDVDATDIVWSESGELVLIVSSDASYALKFDKDIFVEALEAGNIDPEDGVEDSFEVLYDVNDSVTSGKWVGDVFIFTTSTNRLNYLVGGKTYNIAHFDNTAYLLGYLARDNKVYVADKDINVSSYHLSLAVLEYQTLVLRGELEQANQDLLPSIEKNDLLKISRFLEAQEYLEEALQISPDSEQKFEIALKIKDFTTARTIASEENTEHKWKSLGDLALSSFKYKLAIESFQKANDIESLLLLYTSFNDKKHLSELAEQAKAKGKYNVAFNAYYTIGDIPKAIELLNISKRYTEASFLGLTYGADVSKSVENWKADLVKLGKGSIAERISIPESPSKPSQQEDLVDLNDDDDETPQEEAEVDEEVEVEEPVAVELEKPLEPEPTREEEEEEDFEDAASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY09</accession>
    <name>A0A1E3NY09_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_16174</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037192.1">
        <property type="nucleotide sequence ID" value="XM_019180680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY09"/>
    <dbReference type="GeneID" id="30197926"/>
    <dbReference type="OrthoDB" id="10010954at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="270" mass="31021" checksum="CCF2653F972CB961" modified="2017-01-18" version="1" fragment="single">FSNEELFQHAISFANDYLYPNNVEQAKSINSSYFAEDVVGRIDVTRNFEGKELNTEYLFGLFSQLSDNAVTNLIGYSTSYDVYEFLGNCNQYTMSVVNNGTFPSLGNASLPIAVNIWIKLNEYKEIVQYDLTFLKFERLFEAIELAGYQALSGNKTATEIPEEGYKQLQGLLVKSICDVHDHYCQKDYPQYDSKQDCIDYLTKTRLGKDYEGGRNTVWCRSLHQNMIEYRPNVHCPHLGPSGGDMCTDEDTGFLEVTYNYKKTFHNPWMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY16</accession>
    <name>A0A1E3NY16_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_56696</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MSOX/MTOX family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037298.1">
        <property type="nucleotide sequence ID" value="XM_019182786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY16"/>
    <dbReference type="STRING" id="683960.A0A1E3NY16"/>
    <dbReference type="GeneID" id="30200032"/>
    <dbReference type="OrthoDB" id="2219495at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008115">
        <property type="term" value="F:sarcosine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045170">
        <property type="entry name" value="MTOX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961:SF15">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961">
        <property type="entry name" value="PEROXISOMAL SARCOSINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="6">
        <location>
            <begin position="11"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="505" mass="57512" checksum="95FAD42FBF5A47AF" modified="2017-01-18" version="1">MNKTITRDSQIVIVGAGVFGLSNALHLAQNGYKNITVFDRLDFTNNHYTFLEGADTASADLNKIFRARYENEKHYQDLAFEAFEIWQKWDREIELLPEHEAKKYTGLKLLDLCGMLTMDDILSKGSKESRENFEKEGFAPLVYDINNPKDVKRANVAGFGNKVKYGQDMKKKIPQIEGVLDTTSGMLYASRACQYAKYLCEQLGVRFVLGGDKGSFQDYIYKDQEQKQVGGIITKDGRRYLADLVVVNCGPWSTSIVPELDGINEATGGNIFIFKVPEHRKDLRVKYSAKNFPLIGWKTGHAREKEHMGGMMMFPMAEPEGYMKIIVRQTKYTNPIKTKDGKIISIPKTSNSSPPHNYLTKSIVSQAKEWLELFFPDLVEAGIHLESKALWYTDTINNDYIYDFVPGKEGLFVACGGSGHAFKMLPVLGQFLVDKIEGRENLYTQIFKWRYPKDFDKDVNGLKEKLDSKRRYDLQELSGPEDLKFQKKSFSSEIGLSNLQITSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY48</accession>
    <name>A0A1E3NY48_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Luciferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_64171</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the NtaA/SnaA/DszA monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037230.1">
        <property type="nucleotide sequence ID" value="XM_019183681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY48"/>
    <dbReference type="STRING" id="683960.A0A1E3NY48"/>
    <dbReference type="GeneID" id="30200927"/>
    <dbReference type="OrthoDB" id="3979273at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051260">
        <property type="entry name" value="Diverse_substr_monoxygenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016215">
        <property type="entry name" value="NTA_MOA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03860">
        <property type="entry name" value="FMN_nitrolo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011">
        <property type="entry name" value="ALKANESULFONATE MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011:SF16">
        <property type="entry name" value="C2H2 FINGER DOMAIN TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000337">
        <property type="entry name" value="NTA_MOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="6">
        <location>
            <begin position="43"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="490" mass="55414" checksum="BE19B945C47C6015" modified="2017-01-18" version="1">MTNSPDLKKRKVKKQLILSAANVNNSNYVWRDPKDTGREFAHSLEPWIEFAKLAEEAKFHNVFFVDQLSWFDVYGGDFNASAKHGVHAARIDPAAVATAIASHTENVGIVITFSTISEHPYHFARRLASLDLLTNGRVGWNIVSSYLKSMGPNLLNGEPFPEHDERYVKTREFVDVVYKLLLSSWREDAIVYDKEKGIFADPKAIREINHEGKYYNVKGPSITEPTPQGFPLIVQAGTSEKGIDFAAENAELIFVPIHSPKSTTEKIAKIRKLASQKYKRDPHSLKFIAPLIPFLGKTHEEAEQKLEHSLKYEDPYDSLISLGGMIGIDLSGFAFDEPIQFEGEGTGITSITKNVFLKPDGTPRTPREIATFWRSTTRAVGTGEEVAQYIQKLVEETDIDGINFVFPQFPDGFRDVVDILVPELQRLGLAQKEYPVPGGTFRENFWGEKGHVFLDESHPAFKQKWGKGFTKEQFEKRLNEHLKVVNEYRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY71</accession>
    <name>A0A1E3NY71_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_56545</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037211.1">
        <property type="nucleotide sequence ID" value="XM_019182783.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3NY71"/>
    <dbReference type="GeneID" id="30200029"/>
    <dbReference type="OrthoDB" id="5330228at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18070">
        <property type="entry name" value="DEXQc_SHPRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052583">
        <property type="entry name" value="ATP-helicase/E3_Ub-Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059033">
        <property type="entry name" value="C144_05_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45865:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SHPRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45865">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SHPRH FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26021">
        <property type="entry name" value="Ferritin_C144_05"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="342"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="1153"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="1259"/>
            <end position="1417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1442" mass="166008" checksum="E539A6077E747285" modified="2017-01-18" version="1">MAVVNGVVQSLDLKLIQIFNGLGPKRPKKRKASVLDRNGETLYNDDGDDNTEDSLGIKDPTYLTIADTSIKAQGQVPRPPNEESPQQVGSVLQLDDISLNFQNSILIIQNKPRQKYKTKNTMSLEIKDEIYNQIGPHEIKFCRNLKLKHASSVKLSYPKLSIQFLSVEQDHYEIEVLIDYKVELIESLALNTRLTDTQLGYIKDLIKSIAVYPNPPQSNIEINTKFFYDMITQHTESIPKETKPLLVSELDTDLLKFQTTSVKWLLTKENVLYDDESRSVKPSPIWSKSMSNDDEKVLKALDSISFGFKLIKIPFYDEELWYNDYTQNICSKNRAIEFLTDWDFANIGGTGLLAEEMGLGKTVETVALTLLNQRPFNDINQNYFETSLQRMVKKLKSTLIVCPESILHQWCDEIELHAPSLTIMIYKGINSSKQTCTELVEEMSKHDIILTSYSVISREIHNALYNPSSRPKRQRRTPTPRFLELELDPSIDIEPEALESLKQIYQQIDSDRKLIRDRDDHSSPLVMCEFWRIIMDEVQMVGKTLSNVCKIATRIPRFHSWGVSGTPIKNELSDLNSILSYLRIEPFGSLKNWESLVGKPNDFIKLFKNLSLRHTKEMVKSDISIPPQEKYLLSVPFGPIEQNNYEELYKQFLNEVGLDVDGNPIVENWEPSQSYYDYMSHWLKKLRRVCCHANIGNNDHQSGNENSALQTMDRVLKNMIEIAQDRVNQLNRDITLKKIEIGQVYEFEKKPENALDVWSQELPNVEKRIESLQTLSINNDKSKLNLRINLDLLHRLYFFIASAHYQLYNPSITELVPGIDKDMLPHDVDPRLGGDEHISPDVVVYRVLTEQESNHQDLEQEFYTKAQNVRKTLLAEPIKNVSNSVMKLDRNFQLEFIDFIPKSFQTLDTQAFSTRVGTVITQLNSQSSLLNNWLGKLVNAISTPLLDGVTDPDGEEYGESLDDQEKAAAYLDVLQRAIQDRHSLIFGKNDHGDFEKIDAIVESSDSDFKDQLEQEYPKIMMEFSLRHLVIESKTILAGMTVVQDMDPFAKLIVEVRKILELQKENMDSVKKFFKIINDCYNLRITYFRQLQDLSDNVRPFDEYSHANYDCMENLLKLKNKIEIDEKNLTSIHSRIRYLQSLSSAETDESDKLCVVCRSSITIGSLTKCGHQYCKDCLDHWLARHSKCPICKQSITKVDVYNFTFNRDEINVRMITEESNEEMFQVYHKMDKNNLYDINSIKIKNNFGSKVNTIVKQVLWLKKHDPYVQIVIYSQWTQLLQIIGNAFRQNGITYLGSKNTLPRIGKGKKTALKSDIAQFKKNPEITCFLLNAKAEASGLTLVNATHVFICEPLVNTALELQAISRIHRIGQKKPTFVWLFSITGSVEESIILLSTRKKVKEREKLDTEEMTKTVNKLVEANGEHVNPSDLWNSFFSAKIDRIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYD5</accession>
    <name>A0A1E3NYD5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_97121</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036919.1">
        <property type="nucleotide sequence ID" value="XM_019186564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYD5"/>
    <dbReference type="GeneID" id="30203810"/>
    <dbReference type="OrthoDB" id="10635667at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="195" mass="22567" checksum="894BA9A49E3BE4B9" modified="2017-01-18" version="1" fragment="single">MNIGKLKWNKSMQPPKLLSENDVLPKEVFKITPEVAKFLNVCLKPSLTLDSPTELRCAKEYIKSYFIELETFTLDDDPFAKSFHKIPTAIQVPKEGDQQYLEEILDTNDGFALNFDYCKEPTLKPLFTESDFQIPVVDNQKCEHQGVQRTLTTVPKWHLSFPKSLNTEASSLEKFILNEELQKGVEYRLRAIYMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYI8</accession>
    <name>A0A1E3NYI8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_80332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037385.1">
        <property type="nucleotide sequence ID" value="XM_019185046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYI8"/>
    <dbReference type="GeneID" id="30202292"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="346" mass="40178" checksum="080C265D97B594DD" modified="2017-01-18" version="1">MAPPRSHKTFISFADRLTPSTIIESLGISPETFTQLQRKINFDGRCEDVIVQLIRERIYYVILIEKFAHSFDGGQNGKNAEKIGELIGFSKLQKKDEFNQVMKLKAEEYPDFFHKSSRDIIDVAVLKKSLKINIFAKLKSMGKRWLGFGPNYKTKESSHLQNVSKMFHYLCCIRTTSPLTSRTIGTIVLPITRFEQIRTIKITSRKTTRKPKSNEKTEEIKPKDDKRIKEESKTEREANERLDKINKMKEESIADRLSPAVIQESLGVSSELFAELQKKIDFHGDPFNVISKLVEERVFPFTMCYRYERAYGAEHMEGYTQMTLLEFEVIYGILEEHDDDIVNTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYK6</accession>
    <name>A0A1E3NYK6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_80436</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037496.1">
        <property type="nucleotide sequence ID" value="XM_019185062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYK6"/>
    <dbReference type="STRING" id="683960.A0A1E3NYK6"/>
    <dbReference type="GeneID" id="30202308"/>
    <dbReference type="OrthoDB" id="276515at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012430067" description="Endonuclease/exonuclease/phosphatase domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="39081" checksum="5C5508E9FE768345" modified="2017-01-18" version="1">MLFLNLLTLSTAALAADTIRLQNWNLRFDSKHDGISVEDTIKSLNSSVPNDDEVTYYADYGEESWSKRRIGIANDVIFNKAHIFNVNEALKRQVDDLQDLLSKISGTNWSYVGVGRDDGHEKGEYEAIFFDPNHIKVHDFHTIWLSNSPFEPSQYPGAGSYRSATIGHLQTKKGTNFTMINCHLDDKSDDQRKLGASMLRHIGAYEYMNSDGPVFLTGDFNSKSEGETSGAYKIATGSAPLVSMNSTFVEKYNSDISDSFTWGDLYGQVDPLRRLGHFSTWDGFKKWGDVNALGGRIDFQFAGKPNSNAKNVPNKYHKALTHKVGETFYDLEWHLSDHRPVISDISLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYS9</accession>
    <name>A0A1E3NYS9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">rRNA methyltransferase 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_34456</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037482.1">
        <property type="nucleotide sequence ID" value="XM_019182188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYS9"/>
    <dbReference type="STRING" id="683960.A0A1E3NYS9"/>
    <dbReference type="GeneID" id="30199434"/>
    <dbReference type="OrthoDB" id="270651at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008989">
        <property type="term" value="F:rRNA (guanine-N1-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18105">
        <property type="entry name" value="SpoU-like_MRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047182">
        <property type="entry name" value="MRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047261">
        <property type="entry name" value="MRM1_MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004441">
        <property type="entry name" value="rRNA_MeTrfase_TrmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00186">
        <property type="entry name" value="rRNA_methyl_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103:SF1">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="RNA 2-O ribose methyltransferase substrate binding" evidence="11">
        <location>
            <begin position="112"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ58275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="392" mass="44155" checksum="EF4B73D11B60FC59" modified="2017-01-18" version="1">MLSVRRFSTSTTLYKRILPAVDVSHITSPGNGRPEGFERNFPIARRQKAWEKTGEDKETYFKRKHAHTHAMQKKRADAESNDFNDMNDFESRPRREKREVYSGIRPSPLSEFIYGTNSVFAALSAKRREYFSRLLTFNPKPEDQEIIDLAKKENIRVQRAKDKNELNILSNNGVHNGFVLETKPMTPQPIHYLDESNFEKGTYRIIDDELGEKITRECKTENPHPFGVYLDEISDPHNVGAIIRSAYFLGADFVVVSSKNCAPLSPVVSKTSVGAIEFLPIYSATSPLSFLDKSREKGWIVVSGAEASHPTDSKINQTLRSKEFEYGALNSLLGESPVLLVVGSEGKGIRTTMKLRSDYLVQIASHQNVNPMVDSLNVSVATGLLIQKIMDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYY8</accession>
    <name>A0A1E3NYY8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_106765</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037638.1">
        <property type="nucleotide sequence ID" value="XM_019180481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYY8"/>
    <dbReference type="STRING" id="683960.A0A1E3NYY8"/>
    <dbReference type="GeneID" id="30197727"/>
    <dbReference type="OrthoDB" id="1666796at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF111">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009027330" description="Transmembrane 9 superfamily member" evidence="7">
        <location>
            <begin position="20"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="351"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="430"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="471"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="511"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="552"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="582"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="621"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="652" mass="74244" checksum="6BE0827867ECAB59" modified="2017-01-18" version="1">MTLISQLLLTTTLLTSALARKYFVKDEFVPITVKPLTPGGNSYDNKLYLYPIDYYNPTLGSCLPVDQMDPYGPESNSSYIYQEDDFFGNHFSNAPISTTFLNKTAKCASMCDRNYKKENVTLINDLIKKNYKLNFFIDDVPVGRELYDSQTKKFYIDNGVPLGYLDMDRIPRLFNHFQFVVYYVSYGLEYEILYSTVSTRSISRVPIVPLSCELTQPLLLRYSDYSFNGNRISYDIIWKEVHVNKWNNKWELYTNDIVQPFISNAVVIIFTILTIIISFYTYLTISNAFNTEKLEFIYKTRQQLKTVDESTNNGTSSEETKSSDDNIIEDFEFSWPSLINDVFRLPEFSPLLYWLVSNGIHVTVIASIIIILLAIGLLDTHSFDDDVSYTAAAVHTITSPLPALINCYFFKKFDSSSPSTVESFKKKLTLTVLLNSFGVPAILFVLINITNKTFASVDSPTNISLCFFRTLATYYILITLVSFFAGLRFLKVQQYPSSDIAKKISSPPITFQTIPNMFISGIFPFGVILIPLSSGYITLWYSHFYSNSTLTISYILLLSLTVSISILNIYHSLSIGNWKWQWYSFLSGFAVGVYTFAYSFYLTKFKFGDHASLVIFLLQNITISSLLGLIGGSVAFSASYIFVKNLYSGIVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYZ9</accession>
    <name>A0A1E3NYZ9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_33570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037606.1">
        <property type="nucleotide sequence ID" value="XM_019182089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYZ9"/>
    <dbReference type="STRING" id="683960.A0A1E3NYZ9"/>
    <dbReference type="GeneID" id="30199335"/>
    <dbReference type="OrthoDB" id="361029at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18086">
        <property type="entry name" value="HsC9orf114-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003750">
        <property type="entry name" value="Put_MeTrfase-C9orf114-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150">
        <property type="entry name" value="CLASS IV SAM-BINDING METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150:SF13">
        <property type="entry name" value="METHYLTRANSFERASE C9ORF114-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02598">
        <property type="entry name" value="Methyltrn_RNA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="319" mass="35843" checksum="80D4D55C8FDFF186" modified="2017-01-18" version="1" fragment="single">EKKQKISPKTINYTLCIPTSIISRSNAKNLQQATGILYQIAKVATIYNVGEIIKFDDIDSNAKKEAELKNQVSEDAMLIATILQYFVTPPYLTKTLFKTKFFKNFQYAKTFPKLTTLPFMSDEVESKYREGLTVTMGKSSKPLTKNKKSQPLKNTKYVNIGYDKYLELSGQQVPTNVRVTVDTETKKVVSPLEAYQDQVGAKSSYGYHVRIAKTFTSIFTGSSYPDGYSQSIYVNSGDYFHHHKDVELKEIGSIDTSKESNLLLVIGKWNDVEYSFSQDAKNLEGVSNAKEFFDGQVKIPSGTKVEDGSMIALTKLVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ29</accession>
    <name>A0A1E3NZ29_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_85126</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Fluoride channel required for the rapid expulsion of cytoplasmic fluoride.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>fluoride(in) = fluoride(out)</text>
            <dbReference type="Rhea" id="RHEA:76159"/>
            <dbReference type="ChEBI" id="CHEBI:17051"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:76160"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the fluoride channel Fluc/FEX (TC 1.A.43) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037169.1">
        <property type="nucleotide sequence ID" value="XM_019185841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ29"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ29"/>
    <dbReference type="GeneID" id="30203087"/>
    <dbReference type="OrthoDB" id="409792at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903425">
        <property type="term" value="F:fluoride transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003691">
        <property type="entry name" value="FluC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259">
        <property type="entry name" value="FLUORIDE EXPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259:SF1">
        <property type="entry name" value="FLUORIDE EXPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02537">
        <property type="entry name" value="CRCB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="337" mass="37000" checksum="4D559F63DADFB9F5" modified="2017-01-18" version="1">MDFDQLFGIMLESLPNSYTKNSNIVFKEIKLLSILAFFAILGVLARKGLTVLTTYSGAFVGGVIWANFTACVVMGMLIQSQRAWKDYAPKATVALYTGLATGFCGTLSSFSSLILEAFVKSADISIGKYYHYPNGAYGIMECASVFITQMCVSIAGLHFGKHLIKLYDPKIPHMKLIENLAALCGILAWIAVICLIAIRNWRSWTFSCIVAPIGCWTRFYVSKLLNPKFKRFPLGTFTCNVFATLVLAMLNLLSRGKLPNGGRIINSVLACHMIQGLDDGFCGALSTVSTFVVELYTIKSPNSYKYGFVSLAVSYIGMLLILGSYNWTQGLTDPLCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ64</accession>
    <name>A0A1E3NZ64_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Serine/threonine-protein kinase SCH9</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_34153</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037738.1">
        <property type="nucleotide sequence ID" value="XM_019182156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ64"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ64"/>
    <dbReference type="GeneID" id="30199402"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904828">
        <property type="term" value="P:positive regulation of hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060963">
        <property type="term" value="P:positive regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045945">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901494">
        <property type="term" value="P:regulation of cysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047484">
        <property type="term" value="P:regulation of response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090153">
        <property type="term" value="P:regulation of sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11651">
        <property type="entry name" value="YPK1_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000116">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="110"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="319"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="581"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="22"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="93"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="163"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="673"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="22"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="207"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="683"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00074478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ58531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="716" mass="80535" checksum="C84537AA6D8560C9" modified="2017-01-18" version="1">MVDFAKSLFGFGGFHKNAAVSNERSQSLNSPTPPVDQTQQDEKLHHQQPSMSSSGTLFPNFQQTSNPTTALSDNSFVYGTTAKPNQYQPQEVFSTSAGNNESSANPKPILPSTISDDGTPNSNIPRGKLKVTIIEAKNLLTKNANAQPYVVCTFESSEFITHGPESVDNHPASVGNSNGGHKRDKSIPAATSKRPLYSRQSSSQFLNRQRYQQPNNSNNNTTHSNQDESSLPNSSNPVWHHETIFDVIGSHSELDISVYDSDETFLGHVRLRPTTLSNHKNIEWFKLNSRVDGENIEGEIKVSWDYSITRKRHYGPEDFEMLRLLGKGTFGQVFQVRKLDTDRIYAMKVLSKKVIVRKKEIAHTIGERNILVRTAGASSPFIVGLKFSFQTPTDLYLVTDYMSGGELFWHLQKEGRFSEERARFYIAELVLSLEHLHDNGIVYRDLKPENILLDANGHIALCDFGLSKADLSTGDTTNTFCGTTEYLAPEVLLDEAGYTKMVDFWSLGVLIFEMCCGWSPFYAENTQQMYKNIAFGKVRFPKEVLSPEGRSFVKGLLNRNPRHRLGSQNDARELKAHPFFQDVDWEALKQKKIPPPFKPHLSSETDTSNFDPEFTNTSASLINKQFHLASTPLSPGVQANFKGFTFVDESTMEDFHTKRAQQASFREFGSFIPGNPHLPPTEDVIEDEEEPNEDNFVDNDGDHEMDDQFVNGQFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZH7</accession>
    <name>A0A1E3NZH7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_14311</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037665.1">
        <property type="nucleotide sequence ID" value="XM_019180604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZH7"/>
    <dbReference type="STRING" id="683960.A0A1E3NZH7"/>
    <dbReference type="GeneID" id="30197850"/>
    <dbReference type="OrthoDB" id="5404651at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045114">
        <property type="entry name" value="Csn12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732:SF8">
        <property type="entry name" value="NUCLEAR MRNA EXPORT PROTEIN THP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732">
        <property type="entry name" value="UNCHARACTERIZED PROTEASOME COMPONENT REGION PCI-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="427" mass="49895" checksum="5BC7A550BDAF74CF" modified="2017-01-18" version="1" fragment="single">TQLGSLLSINLSNQSIASQIASLQIELQSNQLDIDQLVEQQSYFDGDWNAFTSIVTSYLKFAKDVEPNSILKSHDLLLNYYNDLSIGFLNNSFGNHLSVLIYETTKILVPMMKKIDKYLNLLNNGKKFKRLIFISTILTKIFNHLRSLKGDSNKKNLIIFIVNNLNKIYFIINSPLLCANIFANMNLLNLNFNKYPKSQQIEYRYILGRYYMIKNQLYKAYHHLNWSYMNSHKVYNPQNTIRILRYLIPISILIGKIPSLQILNISIEFQQNYIPLIKYLKQGNHLQFQKTLYDNQLYFKSKFILILLIQRSKVLIFRNLFYKIMTITQSTRFSYDELATSLLKSIGTQQQQSQTLGNDSIMYQILNEPIDDLFVENVCVSLIENDLIRGNVLSRARTLVLSKKEPFTDIPMVFKRKFGVNSSENWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZI7</accession>
    <name>A0A1E3NZI7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_79157</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family. Monothiol subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037816.1">
        <property type="nucleotide sequence ID" value="XM_019184844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZI7"/>
    <dbReference type="STRING" id="683960.A0A1E3NZI7"/>
    <dbReference type="GeneID" id="30202090"/>
    <dbReference type="OrthoDB" id="423313at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005796">
        <property type="term" value="C:Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000093">
        <property type="entry name" value="Glutaredoxin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011899">
        <property type="entry name" value="Glutaredoxin_euk/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02180">
        <property type="entry name" value="GRX_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694:SF5">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133588" description="Glutaredoxin domain-containing protein" evidence="5">
        <location>
            <begin position="27"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaredoxin" evidence="6">
        <location>
            <begin position="124"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="70"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="70"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="227" mass="25090" checksum="797D4F7E8EA4BB3A" modified="2017-01-18" version="1">MVSSRKSRILALSAFLLTVVFIVIQTHNHKVTNTTVAGSKSQGTNIEPTTKIGVIDESKIKSNEEKIKNKLQSNEDKDESNVEIKQKQQQKPNKDGTKDNTNTPVSSEFDAASELQSILGLSPVVIFSKTYCGYSQTLKNLLKSEYEINPPPTIVELDKHKNGRQLQDFIGTKTGRKTVPNLFINGVSRGGSDDIKKLHEEGKLLNSLNLWGDKDVKVNKINAPSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZR2</accession>
    <name>A0A1E3NZR2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Anaphase-promoting complex subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_54507</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the APC10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037775.1">
        <property type="nucleotide sequence ID" value="XM_019182739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZR2"/>
    <dbReference type="STRING" id="683960.A0A1E3NZR2"/>
    <dbReference type="GeneID" id="30199985"/>
    <dbReference type="OrthoDB" id="24948at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08366">
        <property type="entry name" value="APC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016901">
        <property type="entry name" value="APC10/Doc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004939">
        <property type="entry name" value="APC_su10/DOC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12936">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12936:SF0">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03256">
        <property type="entry name" value="ANAPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028841">
        <property type="entry name" value="APC10_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01337">
        <property type="entry name" value="APC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51284">
        <property type="entry name" value="DOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 6" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DOC" evidence="7">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR028841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="174" mass="19470" checksum="B4E24F08077C57F0" modified="2017-01-18" version="1">MEELKLVDIGALGAWSVSSCKVGSGIKELREDNPESFWQSDGAQPHYLDIHFSKKVSISRISIFTDFARDESYTPSKITFLAGNGFHDLIEVTTVELNQPSGWTHIPFDEEQEIGGKCLKTFLVRLLILANHQHGKDTHLRAVKIYSPMTTYSLNSGILGSFTSRKLQSEAVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZW1</accession>
    <name>A0A1E3NZW1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_33525</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Transcription factor that is part of a SLN1-YPD1-SKN7 two-component regulatory system, which controls gene expression in response to changes in the osmolarity of the extracellular environment. Under low osmotic conditions, phosphorylated and activated by the phosphorelay intermediate protein YPD1. Also activated in response to oxidative stress, independent on the two-component regulatory system. Regulates heat shock genes in response to oxidative stress and genes involved in cell wall integrity in response to osmotic changes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the SKN7 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037737.1">
        <property type="nucleotide sequence ID" value="XM_019182083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZW1"/>
    <dbReference type="STRING" id="683960.A0A1E3NZW1"/>
    <dbReference type="GeneID" id="30199329"/>
    <dbReference type="OrthoDB" id="424572at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008361">
        <property type="term" value="P:regulation of cell size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900101">
        <property type="term" value="P:regulation of endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000304">
        <property type="term" value="P:response to singlet oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000380">
        <property type="entry name" value="Transcription factor SKN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014402">
        <property type="entry name" value="Sig_transdc_resp-reg_Skn7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002595">
        <property type="entry name" value="RR_SKN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="15">
        <location>
            <begin position="303"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="209"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="427"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="215"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="265"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="446"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="458"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="13">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR002595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ58530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="468" mass="52682" checksum="60A7AA2B4CEE44E4" modified="2017-01-18" version="1">MSLNASLGIPLTNASSTTSGSTDFVKKLFLMLEDNSYDQIVRWTSKGDSFVVLDTNEFTKVILPKHFKHSNFASFVRQLNKYDFHKVKLSNEEKQLNEYGEGAWEFKHPDFRRHDREALETIKRKGPAQKRSNEDTAQETIIKLEDTVSKLTHDQKILFDELKVMNSKYTNVIESIITLKNTNDRYFRSINTLVNCLASNGIKLPQLDLPPIDSPMIQQQAPQPQPQQPKVQMMIQQPQPQQAHQPSLHRSSNSQGSPQGLQQLPPQPNQQPLPAQPQPPQPQAQDSRPESQQLQSPLKPGFHVLLVEDDNVCIQLCQKFLVKYGCTVEVVTDGLTAISALETSRYDIVLMDIVMPNLDGATATSIVRNFDNETPIIAMTGNIDTNDLVTYLRHGMSDILAKPFTKNDLYSMLQKYLSKRVPLLYQQQGSVPPPPPQAQVDPNVSHGGSISNSVSPNENDHINKKQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZY7</accession>
    <name>A0A1E3NZY7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein MSS2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_79195</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037855.1">
        <property type="nucleotide sequence ID" value="XM_019184852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZY7"/>
    <dbReference type="STRING" id="683960.A0A1E3NZY7"/>
    <dbReference type="GeneID" id="30202098"/>
    <dbReference type="OrthoDB" id="1658288at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="380" mass="43797" checksum="4656920C67DC1147" modified="2017-01-18" version="1">MMFGGRVVVGSKSVFLRCFSTRRPPFYEVFPKKTVMNRILFDLDSRHNYGKLYKVFESVYQKLDTEEEPEIPKFINASDLMIMKKTLENIRIRSHTTNRNLLDLENELTEKAAEYGNNDAVALLAFEAIMNKSSPQDDRAYAKKLVAKLLELKHPLTLKMSGDLCVQNQMLAQAEKFYNDFLELESDTFLASEVYKALGKLNFQKPDLIKSKQYFEQSIKTGPIDKISECHYYLGQLNAHDPERARFHFEASASQGFLQSFQLLGFLELNYFDNSFKAKEWFKLGAELSDIHSIIGLFDCFIKEDYLKAAKKTYSTLEVLLTKDGDDDKLKVWHHFNSNRKNGVEKVKDLATSVESTFKGAQESIELSNTAPSQENRWNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P037</accession>
    <name>A0A1E3P037_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Signal recognition particle receptor subunit alpha homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Docking protein alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_93246</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP-binding SRP family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037922.1">
        <property type="nucleotide sequence ID" value="XM_019186409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P037"/>
    <dbReference type="STRING" id="683960.A0A1E3P037"/>
    <dbReference type="GeneID" id="30203655"/>
    <dbReference type="OrthoDB" id="1727884at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005785">
        <property type="term" value="C:signal recognition particle receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000566">
        <property type="entry name" value="Signal recognition particle receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF1">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="11">
        <location>
            <begin position="355"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="24"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ58715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="382" mass="41566" checksum="B3348D8A06A3C0CD" modified="2017-01-18" version="1" fragment="single">KSKAKQQKKLRKWGADGMVEEEAGYTLDFSTEGTPQPSTPKGDGDEHAVSIDTKNFGDKDLKTGAFLVKDLNDEIDAILSSSKQKQEVKEPFGFLKKFTGELQNHLIRKNVAPEVASHLTSNVEKALVGSKTKNWTSIQATAKEALAKALTKILTPGTSVNLLQDIRSNKKPYVISVVGVNGVGKSTNLSKLAFWLLQNDFKVLVAACDTFRSGAVEQLRVHVNNLKKSSESQDQIELFEGGYGGSDLVAKIAKNAIDYAQKNDFDIVLMDTAGRRHNDSRLMAPLASFAKAANPNKIIMVGEALVGTDSVQQAKNFNNAFGAGRTLDFFIISKCDTVGDLIGTMVNMVYATNIPILFVGVGQTYTDLRTLSVEWAVNILMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P061</accession>
    <name>A0A1E3P061_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquinone biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_63140</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Membrane-associated protein that warps the membrane surface to access and bind aromatic isoprenes with high specificity, including ubiquinone (CoQ) isoprene intermediates and presents them directly to Coq7, therefore facilitating the Coq7-mediated hydroxylase step. Participates in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the COQ9 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037837.1">
        <property type="nucleotide sequence ID" value="XM_019183495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P061"/>
    <dbReference type="STRING" id="683960.A0A1E3P061"/>
    <dbReference type="GeneID" id="30200741"/>
    <dbReference type="OrthoDB" id="619536at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.10">
        <property type="entry name" value="Tetracycline Repressor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013718">
        <property type="entry name" value="COQ9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012762">
        <property type="entry name" value="Ubiq_biosynth_COQ9"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02396">
        <property type="entry name" value="diverge_rpsU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21427">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21427:SF19">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08511">
        <property type="entry name" value="COQ9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 8" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="COQ9 C-terminal" evidence="9">
        <location>
            <begin position="152"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="250" mass="28984" checksum="F8D8C5C82818FA31" modified="2017-01-18" version="1">MLFKQLINPSSLRCSTRFIKPSILSRQYHSKDHKDVDEFSLTKPQTEILEKALAYVPKYGFQHKAILEASREVGYSDAIQSLFSHGTYDLIHYHLVKQRLNLQKYLQTEEFLKLNELGKLKFLVKKRLLANKPYVEHLSQLQSYLILPPYFKQSSEELHNLSDDIVFFAGDKSNDFAWYTKRLSLSSSYVAFELFMSNDKSEGFQNTLQLVDKRLDSIDGLGGFYNDLEEFINYGFHSGINLIKSQASRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P086</accession>
    <name>A0A1E3P086_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">26S proteasome regulatory subunit 7 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_79295</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037955.1">
        <property type="nucleotide sequence ID" value="XM_019184869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P086"/>
    <dbReference type="STRING" id="683960.A0A1E3P086"/>
    <dbReference type="GeneID" id="30202115"/>
    <dbReference type="OrthoDB" id="1937997at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045899">
        <property type="term" value="P:positive regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000005">
        <property type="entry name" value="26S protease regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000037">
        <property type="entry name" value="26S protease regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000027">
        <property type="entry name" value="26S protease regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048723">
        <property type="entry name" value="OB_PRS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21236">
        <property type="entry name" value="OB_PRS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="213"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ58748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="438" mass="49183" checksum="1C75AECDCF4D69E8" modified="2017-01-18" version="1">MPPKADWEKYTAPVEEEENKPEEKIVPLSEGDIQVLRTYGVAPYAKKIKEVEEDIKTLEGKIKEKIGVKESNTGLAPPHLWDVLADKQRMEEEQPLQVARCTKIIKSDEGEDKSKYVINIKQIAKFVVALGEKVSPTDIEEAMRVGVDRTKYEIQLPLPPKIDPSVTMMTVEEKPDVTYSDVGGCKEQIDKLREVVELPLLSPERFVKLGIDPPKGILLYGPPGTGKTLCARAVANRTDATFIRVIGSELVQKYVGEGARMVRELFEMARTKKACIIFFDEVDAIGGARVDDGAGGDNEVQRTMLELITQLDGFDPRGNIKVMFATNRPNTLDPALLRPGRIDRKVEFSLPDLEGRANIFRIHSKAMSVERDIRWELISRLCPNSTGAELRSVCTEAGMFAIRERRKVATEKDFLKAVDKVIKGNLKFSSTSRYMQYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0A3</accession>
    <name>A0A1E3P0A3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_80443</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037504.1">
        <property type="nucleotide sequence ID" value="XM_019185063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0A3"/>
    <dbReference type="STRING" id="683960.A0A1E3P0A3"/>
    <dbReference type="GeneID" id="30202309"/>
    <dbReference type="OrthoDB" id="4090114at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033768">
        <property type="term" value="C:SUMO-targeted ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038886">
        <property type="entry name" value="E3_SLX5/Rfp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28042">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE COMPLEX SLX5-SLX8 SUBUNIT SLX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28042:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE COMPLEX SLX5-SLX8 SUBUNIT SLX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="415" mass="47311" checksum="02B67CAF7E41716F" modified="2017-01-18" version="1">MANREVIDLDDSDGEDDIEVIFERSREGTGASRSSDVRINNVIPVERYLNNNENDDDVEIVEERINVPEVPSTPSYVIHSPNGPIPVYENREGSGSAGPERRSFQNARGPIPTQSYRNPQEVAGRAEQMRREEQIRAQQARQGRPPPPPVQRPRRAPPAQRRPVRGYPPLPPTRQMRNRRGPGSSFLDMMLQMDNFPLAFNSIFFNEEDDDDDPNFYPGMAFPHHHHHLHDDGGVDQHIMNIIEQRENQEADKKRNINESATKAYHDYCEEKIKSIKAPYTTFLDPEEDYVCILCGVTLGEGLPEEFRGNIKHAKLEKLQEENDVMAPYQALNLITDADRDLAKRIFMSTCGHTYCGRCVKNISGVKAILKEKKNKFKKTDKDIDNPFIYAPSKCIAPGCNIGLVAKRRFNEIFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0F1</accession>
    <name>A0A1E3P0F1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_79492</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038153.1">
        <property type="nucleotide sequence ID" value="XM_019184904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0F1"/>
    <dbReference type="STRING" id="683960.A0A1E3P0F1"/>
    <dbReference type="GeneID" id="30202150"/>
    <dbReference type="OrthoDB" id="5392646at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048401">
        <property type="entry name" value="SLS1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032741">
        <property type="entry name" value="Sls1_KH-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048400">
        <property type="entry name" value="SLS1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14611">
        <property type="entry name" value="KH_SLS1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20778">
        <property type="entry name" value="SLS1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20776">
        <property type="entry name" value="SLS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SLS1 N-terminal" evidence="3">
        <location>
            <begin position="107"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="SLS1 first KH" evidence="2">
        <location>
            <begin position="194"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="SLS1 C-terminal" evidence="4">
        <location>
            <begin position="374"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ58946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="681" mass="77744" checksum="2DC9B5800EE97D44" modified="2017-01-18" version="1">MLSLRGRIRAFHTFGRLAQAPPTNNLYSFVKDEPSSIEPEQKEEDPSVLRLRGKKKAPGKQNLVILNAKQSNIFHKLPNHTKIAPGFHVLDPIEFETSRKRLLQFKSEVPLDQIISSIQYIKPDASAVSAVRYRQIKDDIASAYSISQLKEFIKKYFPDIKVISSLTKKKTIEKILKDCWKLEESDEISSAQDLLIQRSFDLSPAEIFILLAQNPMTSWLRANVQVIMIPSDRQLIVRASAQHVAYIEMALSRIFHNIVTGSINLEQITELYKNQGEDLPLDVIQRLSGVYMEKSSLDTTSTSPEQKVIASPETTPTSTNNTLYDYNMSALGRRRFSLAKRLMLWALDFNPFVKTNIHIKYNKKSTWFKNILSETMPWIHKNKTWVRLRAPKGPHKSATELKAPEIDYQTIHNELNTPATSNKAITDAPQSVIAVNFGHLLYDESTLKDKTPQTFFQTTLPHIRDTVHSLETFGEIEEADQELVSADRHEYHAQIKLVPSPFNNAQNYLKYPPLEFWIEINDKHKGDVTTLQVLRIINEQNSIVSLPNHPCDLKFLRSDSEPLLEGYNGSDDWLADQPGIKDFLKSSNLDFSGRPGLKIADYVDVKMPGTDEVIRYDYVTMAYRKHLTMDYKGKLLQYSIVEGGRLGGRTSEALLVGKVDEITLDEFKEFVDDSVNFTKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0I1</accession>
    <name>A0A1E3P0I1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 15">Protein disulfide-isomerase</fullName>
            <ecNumber evidence="5 15">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_93648</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Participates in the folding of proteins containing disulfide bonds, may be involved in glycosylation, prolyl hydroxylation and triglyceride transfer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="5 15"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038138.1">
        <property type="nucleotide sequence ID" value="XM_019186423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0I1"/>
    <dbReference type="STRING" id="683960.A0A1E3P0I1"/>
    <dbReference type="GeneID" id="30203669"/>
    <dbReference type="OrthoDB" id="427280at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02995">
        <property type="entry name" value="PDI_a_PDI_a'_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02982">
        <property type="entry name" value="PDI_b'_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02981">
        <property type="entry name" value="PDI_b_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000139">
        <property type="entry name" value="Protein disulfide-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000154">
        <property type="entry name" value="Protein disulfide-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000017">
        <property type="entry name" value="Protein disulfide-isomerase A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005788">
        <property type="entry name" value="PDI_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005792">
        <property type="entry name" value="Prot_disulphide_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01130">
        <property type="entry name" value="ER_PDI_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01126">
        <property type="entry name" value="pdi_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF132">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11 13" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009027336" description="Protein disulfide-isomerase" evidence="15">
        <location>
            <begin position="28"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="16">
        <location>
            <begin position="23"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="16">
        <location>
            <begin position="333"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="13">
        <location>
            <begin position="66"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="13">
        <location>
            <begin position="396"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR605792-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ58931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="507" mass="56574" checksum="E51D93B3A2014148" modified="2017-01-18" version="1">MKFFSALNLKTLSSVLAVVAVSSAAGAEEDAIASPDSAVVKLTSETFSKFLTENPLVLAEFFAPWCGHCKALGPQYSQAADILEAKDIKLAQIDCTEEQELCQEHGIRGYPTLKVFRGESEPSDYEGPRSADGIVNYMIKQSLPAVSLIDDSTDLDDFVTEATNAVIIETGVKQNETFYKLADLHRDDFSFVQTKAPEYAKKYGKDKLLIFLQDSKEPIVYKGDETYEHLVDWLSVETKPFFGELDGSTYQSYVSSNLPLAYLFYNTPEEREQWSKTIVEAAKENRGKLNFVGIDASKYGRHAENLNMDQEFPLFVIHDLANNKKYGFPQDQELTTKELPKFLKKYTKGEIEPKVKSEEIPAKQETSVVKIVGKTHDSIVNDETKDVLVKYYAPWCGHCKRLAPTYEELGDLYQASKEGKDKVVIANVDATLNDVDVDISGYPTLILYPAGDKSNPVVHQGGRDLESLVSFIKEQGTFKVDANKLTKDAEPVKEEAAKESDVGHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0M7</accession>
    <name>A0A1E3P0M7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cleavage and polyadenylation specificity factor subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_31963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038085.1">
        <property type="nucleotide sequence ID" value="XM_019181905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0M7"/>
    <dbReference type="STRING" id="683960.A0A1E3P0M7"/>
    <dbReference type="GeneID" id="30199151"/>
    <dbReference type="OrthoDB" id="277288at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005849">
        <property type="term" value="C:mRNA cleavage factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016706">
        <property type="entry name" value="Cleav_polyA_spec_factor_su5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13047">
        <property type="entry name" value="PRE-MRNA CLEAVAGE FACTOR IM, 25KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13869">
        <property type="entry name" value="NUDIX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="278" mass="32360" checksum="0D3772DEC31C6CDD" modified="2017-01-18" version="1">MATEVSTEVSTTTATQPETKRRKFSSIPPRTIAPFTDNQPPVKLLYPLSNYAFAPKTINQYQAHIAKESETEANLLKNGKIYKQKTFKSDQIPNNNLYFKNLKNYNDLYGTRRYLQFLILVAGEKEPIVMIFKDKSDKFLIPGGYLNHDESETDGAKRILKELFDDGEDAEEEEITMGETVGRWWRTDLKPNVYPYLPRHVTRPKELIKVNLVNLPKNKKFKFPEYFKNFHPYILVDLYDSSEPELKAIPSFLSRFSFGCINDKGEITNEITQEEFKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P110</accession>
    <name>A0A1E3P110_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CMP/dCMP-type deaminase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_84803</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family. ADAT3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038376.1">
        <property type="nucleotide sequence ID" value="XM_019185782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P110"/>
    <dbReference type="STRING" id="683960.A0A1E3P110"/>
    <dbReference type="GeneID" id="30203028"/>
    <dbReference type="OrthoDB" id="3180714at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052718">
        <property type="term" value="C:tRNA-specific adenosine-34 deaminase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF156">
        <property type="entry name" value="INACTIVE TRNA-SPECIFIC ADENOSINE DEAMINASE-LIKE PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="3">
        <location>
            <begin position="158"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="312" mass="35819" checksum="B75BEAD049AB7EC6" modified="2017-01-18" version="1">MVKKKQNKLKIDYEKCIIEDRLLQIKNQAYVNDLKLVKVWTIEISPKDSPKVLVLVRQLSSQDPVSLVHLKRIQPIDGMLHVVLCSTEWLEESQIALKLSNMDFNYNNLCMKLVPGHCPGTKELSQDWGKKYWPLVWKGNPNDQILNDVKIDISLIKNHLDLIHSLSVDSTKQGLPIITCIVDPLNNQLLATTRDLRHIHPLKHSIMQCIETVSQDEQRKRGEDSNSQHYLCYGYHVYTTHEPCSMCAMALIHSRVSRLIYLKSMPSTGASKPSSGEGYTIHDHKLLNSRFEVWEWTGDEYKDVPGLKDINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P125</accession>
    <name>A0A1E3P125_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription elongation factor SPT4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Chromatin elongation factor SPT4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_55693</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SPT4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038393.1">
        <property type="nucleotide sequence ID" value="XM_019182761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P125"/>
    <dbReference type="STRING" id="683960.A0A1E3P125"/>
    <dbReference type="GeneID" id="30200007"/>
    <dbReference type="OrthoDB" id="248751at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032044">
        <property type="term" value="C:DSIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031934">
        <property type="term" value="C:mating-type region heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000182">
        <property type="term" value="F:rDNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001208">
        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001209">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000232">
        <property type="term" value="P:regulation of rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090262">
        <property type="term" value="P:regulation of transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07973">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009287">
        <property type="entry name" value="Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022800">
        <property type="entry name" value="Spt4/RpoE2_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038510">
        <property type="entry name" value="Spt4_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882">
        <property type="entry name" value="SUPPRESSOR OF TY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882:SF1">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06093">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025023">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01389">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="Spt4/RpoE2 zinc finger" evidence="9">
        <location>
            <begin position="6"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="100" mass="11014" checksum="AE87D4186726CBF4" modified="2017-01-18" version="1">MSSLGNRACMLCGIVESSRRFQDEGCPNCVDLLDVGLSTTSPTFEGLVAIGEPDKSWVAKWLRVNTYLPGLYAVKVMGRLPQDIIETLPYYRPRDGSATD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P144</accession>
    <name>A0A1E3P144_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_79697</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038378.1">
        <property type="nucleotide sequence ID" value="XM_019184936.1"/>
    </dbReference>
    <dbReference type="GeneID" id="30202182"/>
    <dbReference type="OrthoDB" id="3981040at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019021">
        <property type="entry name" value="Mms22"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09462">
        <property type="entry name" value="Mus7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="414"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1504" mass="171055" checksum="F1018166467BF730" modified="2017-01-18" version="1">MTSPHVSDSEDELGTDSLLSLSPQILRAILASKLENNSSFAPKSTVSVIYVVGINRSLLTTGQNSLSTLLKANIEGSFENTLRHSISPTIVPSINGAQGQNGNSNDILNASSEDLSYSENSRRATRRRNVSFYQQHIYTIDSVSYTLRMPLEDATELVKKRPEIIEMYHRGCLEKYLKNGFKLPEPPTARKTTTKASVVDEESTVPEDLESDEKYSSSSSQDSSSDESDASQQQQETVVKFRGHIYRDLDAVSRASGLPKSFFSMQKVQRMEKSVPRKRNISEYQVGIAKRKKAANRPTQAFGNDIFTTGVNSQENEISAAETAIDHEIARDSEGEVVEVGSASVKNAALNAYHDVSSESDEDDVMEIVRRERQLTPDSNLSMFQPTVEDEHVFHKLKYFDGDSLDGEAEVATSELDPMLQPVQRNHKKARNTGASKPHNYSTSSVRSRSARSSSARSLAPKRSGTRKRNHIPSLKRVSKPKPLHHPTSKILSNKVQHLGIQTTNTNYQSIVNLLKEDRPKKTSQPFFHITPDKENLLPRNAGASTLVYEGLSESFALRGLPNQQIYGEARGEVPKDFEKFNNKNLDTLNFKGSSIFKSLNNEELPNIQEAKVTFGKRTFVFSKLFGDSSEKLSEVFQYFVDPSFLKGLSKSDIIESVNQVLCFCLNLDLDEIRSLVGVVDSFKIRIESNIMKKGHVKDIEFYLLSICDLVYDICSKKFNQNHIYGYDFQSKVLATTKLYLEFACHLGGDLFCFKLQETGLFRYSMEIISSNSFSPIVEVLRNLSVPDMKFVEAICLFSAIEQPMWNPVVNVLLLHIKNEDLNGLRSDICYIHKFATRFKWSLTEELLSLVYEALKINRFNNFPSERSFPVIFSEVGINKDTSMNTYLNMLLDYSTKSDKPSSKFLEKIIPVSRGALMDRVTFCNRVNILLCLSVAFKRNFESRINELLAGYQIDQKLDIQIIIKMIESTVKINERNGFVSTFKSLDLVVLKCIEIKELDPIKFFFNALNFKSFKMKTQKNLIIKLSAFKHDGTFLKICEPLIRDYSTLATEKEGFESLKHSFGDISPALAPALWCCINGKLVSEDLANWSRLMSFSDFDDEPFIYTYILRHSGNKIYQLERETFIISLVKNLVGRRYSSSLNSYIREIEKFDSKLINTKGVLVQSQRFQLTLDLIVNVLDNESDYTIKRLLSSLVKFVGSEVKNSISEGNLHYKEYVKRIVEFLDVGCVGYLSNEQELDGLKRELGIIIGPSQRNANARKVNSNSLVLLEQKLFDTVSNKIPLDPQLIRNKLFKDDLFDISISSFYDICCFLSIHAELLTRLPVCWILLAKISESLSVLLQQTKSFSQMDIYCLAKTVKLFHYIFKHKNSNYYNYQLLSSINIYRILSALFNILDGTNDYFVLYDLSFVFMSTSLIFDNEIPDTDSPFLVKVPVDISHCIREANLPIDQNLNSEDLQRKLLHEYEKFMDRFKLSESSGLNDETVYGENSGVQNYEGEVEDEWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P169</accession>
    <name>A0A1E3P169_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptide-methionine (S)-S-oxide reductase</fullName>
            <ecNumber evidence="2">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Peptide-methionine (S)-S-oxide reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Protein-methionine-S-oxide reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_79751</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(S)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:14217"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12315"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:44120"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[thioredoxin]-disulfide + L-methionine + H2O = L-methionine (S)-S-oxide + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:19993"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58772"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="2"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038436.1">
        <property type="nucleotide sequence ID" value="XM_019184943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P169"/>
    <dbReference type="STRING" id="683960.A0A1E3P169"/>
    <dbReference type="GeneID" id="30202189"/>
    <dbReference type="OrthoDB" id="77405at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="entry name" value="Peptide methionine sulphoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050162">
        <property type="entry name" value="MsrA_MetSO_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42799">
        <property type="entry name" value="MITOCHONDRIAL PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42799:SF2">
        <property type="entry name" value="MITOCHONDRIAL PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide methionine sulphoxide reductase MsrA" evidence="8">
        <location>
            <begin position="18"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="185" mass="21359" checksum="38D3979E78B695AE" modified="2017-01-18" version="1">MSVISPTIKYDPSKNDIITFAGGCFWGTERLFKRFYGHGVDGLKVGYGIGQVDDKESLSYEQVCSGEADYAEVLQVSYHKGDQAILKSLCEFFFKVHDPTSLNSQGDDHGRQYRSAIFYHDESQIQVINNVIEEFQPKWDNKIVTVVEKIMKYFDAEDYHQDYSESDLANEKNWIPCETHFVREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1C2</accession>
    <name>A0A1E3P1C2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">2-oxoglutarate reductase</fullName>
            <ecNumber evidence="4">1.1.1.399</ecNumber>
            <ecNumber evidence="5">1.1.1.95</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_84890</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(2R)-3-phosphoglycerate + NAD(+) = 3-phosphooxypyruvate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="1.1.1.95"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(R)-2-hydroxyglutarate + NAD(+) = 2-oxoglutarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49612"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.399"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.399" evidence="4"/>
    <dbReference type="EC" id="1.1.1.95" evidence="5"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038493.1">
        <property type="nucleotide sequence ID" value="XM_019185801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1C2"/>
    <dbReference type="STRING" id="683960.A0A1E3P1C2"/>
    <dbReference type="GeneID" id="30203047"/>
    <dbReference type="OrthoDB" id="1621027at2759"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00196"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061759">
        <property type="term" value="F:2-oxoglutarate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04901">
        <property type="entry name" value="ACT_3PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12176">
        <property type="entry name" value="PGDH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000036">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000041">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050857">
        <property type="entry name" value="D-2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008759">
        <property type="entry name" value="PRK11790.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789:SF1">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="domain" description="ACT" evidence="15">
        <location>
            <begin position="399"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ59286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="469" mass="50883" checksum="1E79C0E31DA08D1D" modified="2017-01-18" version="1">MASPQQIKNLQQTFDRALGSPNAVSTSPSQSFLQNQFRPRGFSTSNQAKPLKPFNTEDMKILLLENVNQTAIDIFNGQGYQVEFYKTSLPEDELIEKIKNVHAIGIRSKTKLTEKVLTAAKNLVVIGCFCIGTNQVDLDFAASKGVAVFNSPFSNSRSVAELVIAEIITLARQLGDRSIELHTGTWNKVSAKCWEVRGKTLGIVGYGHIGSQLSVLAEALGMNVLYYDVVMIMALGNSKQVPTLDELLSKADFITLHVPATPETKNLLSTPQFAAMKDGSYVINASRGTVIDIPALIAAMKSGKIAGAALDVYPHEPAKNGAGLFSDDLNSWASELTSLRNLILTPHIGGSTEEAQSAIGVEVGTALTKYINEGASVGAVNFPEVALRSLDLDQQNSVRVLYIHQNVPGVLKTVNNILGNHNIEKQFTDSQGEIAYLIADISDVDQGDIKSLYEELNDTPYKIAVRLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1M7</accession>
    <name>A0A1E3P1M7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3020 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_79763</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038450.1">
        <property type="nucleotide sequence ID" value="XM_019184944.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P1M7"/>
    <dbReference type="GeneID" id="30202190"/>
    <dbReference type="OrthoDB" id="5595797at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021386">
        <property type="entry name" value="SPP41_DUF3020"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11223">
        <property type="entry name" value="DUF3020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3020" evidence="3">
        <location>
            <begin position="1017"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="609"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="678"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="793"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="897"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="974"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1159"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1291"/>
            <end position="1519"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="465"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="39"/>
            <end position="59"/>
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    </feature>
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            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
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            <begin position="103"/>
            <end position="115"/>
        </location>
    </feature>
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            <begin position="167"/>
            <end position="181"/>
        </location>
    </feature>
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        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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            <begin position="234"/>
            <end position="244"/>
        </location>
    </feature>
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            <end position="255"/>
        </location>
    </feature>
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        </location>
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            <end position="303"/>
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    </feature>
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        </location>
    </feature>
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            <begin position="368"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="401"/>
            <end position="439"/>
        </location>
    </feature>
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        <location>
            <begin position="627"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="678"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="705"/>
            <end position="725"/>
        </location>
    </feature>
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        <location>
            <begin position="793"/>
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        </location>
    </feature>
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        <location>
            <begin position="807"/>
            <end position="816"/>
        </location>
    </feature>
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        <location>
            <begin position="821"/>
            <end position="833"/>
        </location>
    </feature>
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        <location>
            <begin position="900"/>
            <end position="924"/>
        </location>
    </feature>
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        <location>
            <begin position="974"/>
            <end position="990"/>
        </location>
    </feature>
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        <location>
            <begin position="1004"/>
            <end position="1022"/>
        </location>
    </feature>
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        <location>
            <begin position="1159"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1304"/>
            <end position="1326"/>
        </location>
    </feature>
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        <location>
            <begin position="1327"/>
            <end position="1338"/>
        </location>
    </feature>
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        <location>
            <begin position="1339"/>
            <end position="1354"/>
        </location>
    </feature>
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        <location>
            <begin position="1424"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1508"/>
            <end position="1519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1519" mass="168298" checksum="2142DDC654ADD70A" modified="2017-01-18" version="1">MSQEESHNIDQEQDDLALVVQQAMEGFQEQAGQFDGADENQNQNNEINVNNASQGQQQQQHEEFNHDDDDFEKEIQRQLEQQFQQQDAQGANDVGDNTNAHVDNGSGDQDNNNNNNDDEDDFAKEVERQLQQQLDQHQQEFQEHQQQQQQDTNTINDFETHIEDQIRQQLNLNDGQPQDQAAQHVVDESVQNDYEQELQKQIMDALNEQPASEEIRQVDQDQNEQKELNHHTVTDSNTQQGQSFEKQEEPKEQHPQSDQNLSQQEFQNDFEQDLQKHIMDVFAAQQEQHQGPETHQPEQDQQKPLEQQPQSDAGPQDADDFELQLQNSIMEAFNAATDVENTSQEQHQQVEQPIQPAEQEKSEPIPQAQPQFSKVEPQPQQTSTAAPVQSHQPEQPATQHQVPSQSAVPPAAQSPAPAPAQTQPVVQPPAQAQTQVAPQYQPPQPQHPQPEYGDVYEEELQNQILSAFAESEQQYNQQMQQLQQLQAQQAAHQQQSQQPLSQYTDDIDPFRDALEQLVQNVIDAQVEPDNKSSSQQQQQQQNDDEEIDMNQIMQNALAMAVENPNLLLQNLNQLNNNNELNLQQDSSALINQLISSGVNLGEAVQATTAAVKNKKKAPAKPKEKKPPKEKKPRAPRKTPAKKKKDTAVVPSTGVITPQFQPQQQLPSFQQQQGIILPQQQQQQPQALLPSAAPGQQPLQLPPQLPQQQQVQQPTQSQLPQSQLIQPSPPTATKPIDLGPKQPKPAPANSIIDSVKNSAPLLLLTKDRSIDLQNHNAEQKKKASLSIAETLALSRSGMSSGKFSSKLQEPKPVEQVHPKTATVGTSQAEQNLQQTPPPPPPIDRTSQDKNVITASVENALKQLLTAQPPSTPTSSTKASDKQDADMIDPALAELQQKVDPKAPSQEQQQPSSSSSSQAPVVSKPSTPFTPLPVAPGSQGNALINNIGSLLPNLPSNLTSMISSAINSAVSSGKISFNDQNPSTPTEFQDTPKSGKKSKLLINRNETPEQQKERIRLENRERKKRWRDANKLKNQNNDLRARLKKRAIQLFGAEETPKKKEWIESEFEKRKNRRIIKAANLEDLATLINETLSKIDVASLNQETLTQIISQLVTSAFNPPAQSAQQQQHQQEAGNNNYITTTQRNLSPGAVEELAGLPRGSVSSFKVDNQSVPRSQPDDESGKIGDQGNQEKVALPSKEPDLELSSLINNKQREQVETTSTPSPVIGQQVNKLPLKPSMPMKRKSDVLSNFELKKPRFNIPLIPTNMKFDDDVDKGKDVQVTKETVVVKDKPEPTKSVVETGVAKPVEKSIEKPIEKPIEKPVEKPVEKPVASVVSKPSPIVTPTPKEPTKPSPPKPVEKPVAVSKLAPPKPSAGLPLHLQRPIKIPSFRRPEPSRPSPPVPSAAATTSVKPVDKPSPAPSTTPEAAKEPTPKPVEKPSSTPPPPTSITTTTTEKEKESTATTTNSVKLEKPSIPSSTTTPKLTRPKFGMGLRKPPAFQGLRKPGAFQRPPDRDKRKGLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1R4</accession>
    <name>A0A1E3P1R4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_79663</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038341.1">
        <property type="nucleotide sequence ID" value="XM_019184929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1R4"/>
    <dbReference type="STRING" id="683960.A0A1E3P1R4"/>
    <dbReference type="GeneID" id="30202175"/>
    <dbReference type="OrthoDB" id="301415at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990816">
        <property type="term" value="C:vacuole-mitochondrion membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005272">
        <property type="term" value="F:sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005244">
        <property type="term" value="F:voltage-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097553">
        <property type="term" value="P:calcium ion transmembrane import into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056337">
        <property type="entry name" value="LHD_YVC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052971">
        <property type="entry name" value="TRP_calcium_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056336">
        <property type="entry name" value="YVC1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35859:SF5">
        <property type="entry name" value="ION TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35859">
        <property type="entry name" value="NONSELECTIVE CATION CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23190">
        <property type="entry name" value="LHD_TRPY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23317">
        <property type="entry name" value="YVC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="426"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="479"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="508"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="YVC1 N-terminal linker helical" evidence="2">
        <location>
            <begin position="25"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Calcium channel YVC1-like C-terminal transmembrane" evidence="3">
        <location>
            <begin position="253"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="656" mass="74904" checksum="A3BD5559B3392DA8" modified="2017-01-18" version="1">MAEDYLLPLTEDQVNTFTPPSPRQVLKICLNLKYLIDEIIPVQFDPSVVLSPDSKIINNRVLKLALQAAGGQGDGVTGSSSSKYRAVLIFALLKVCGWYWELSSTEIHDSELYDLRASTAQVLAKLIIEQENDDKYLFIQMLCRRYVVNLNTVDSEPLNALELAVDMHSTIVIGSAGYQRCMKWLWRGWIVQSAKDPQSYVLYKDISKSNLSSHFNPDRIKTPLYQYSLEIFFSLIYLALYTIVVNQSDRTVMPVDFWEGAYYFFTCSYIIDELTKLYHVGLNYLSFWNIFNDTMYAIVSGSIILRFLSLTHAHDSPAGIHLDEASFRILSLAAPFMWIRLLLYLDAQRFVGAMIVVIKTMIRESMIFFVLLTIVIAGFLQGFLGLDSADGKRDATYLIVQNLGKTVLGAGDFNAFKRFAPPYAAVLYYFYSFLISVILLNILIALFSSAYSKIYDNAIDEYMALVAQKTLRYIRAPDVCVYVPPLNIIELIFSPLALVLSPKSYSTLNYRIMVICYSPYLSWIALKEARESRRIQYNRIKGLADDANEFDREWDLTDGYQDDYEGIFATGGAESSNERVNEDLRAQYNAERVDPHFNVGKQWYKRVKSIAPPVDQGTTVGVGWELYPLYEKIDQLTNLVEKVVAENKELKEELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2A2</accession>
    <name>A0A1E3P2A2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytidyltransferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_33134</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>beta-nicotinamide D-ribonucleotide + ATP + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038680.1">
        <property type="nucleotide sequence ID" value="XM_019182036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2A2"/>
    <dbReference type="STRING" id="683960.A0A1E3P2A2"/>
    <dbReference type="GeneID" id="30199282"/>
    <dbReference type="OrthoDB" id="5591297at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034355">
        <property type="term" value="P:NAD+ biosynthetic process via the salvage pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02165">
        <property type="entry name" value="NMNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005248">
        <property type="entry name" value="NadD/NMNAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31285">
        <property type="entry name" value="NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31285:SF0">
        <property type="entry name" value="NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="9">
        <location>
            <begin position="47"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="265" mass="30323" checksum="4AD1B432E6C25E7C" modified="2017-01-18" version="1">MSKSPNAQKKFQSQLHKFTETNTSFKVVYSTLSTHITPGTTRLCILDSSFNPPHKGHLSLITKSINHSKDQTCSVLLLLSVQNADKLTPTPASFEDRLDMMCLLADYIQENYKVAVSVALTNHAKFVDKSSVIQDWAKEIKTKELKYLFLVGFDTLIRIFDPKYYKPFGIDEALTDFMQLNEFFCLTRTDEKNDDVDAQHQYVHDIASGKTELPSEWATKIHLVEGDDDYLHISSSTIRKQTNQTNWEAQVVPTIAEYIKQEDLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P311</accession>
    <name>A0A1E3P311_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_62453</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039076.1">
        <property type="nucleotide sequence ID" value="XM_019183371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P311"/>
    <dbReference type="STRING" id="683960.A0A1E3P311"/>
    <dbReference type="GeneID" id="30200617"/>
    <dbReference type="OrthoDB" id="3981028at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="212"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="362" mass="42600" checksum="C32B86EAD8DCEA57" modified="2017-01-18" version="1">MNGNAHQENLEDSIASLLSSSLIQEPKDDSVIINSNYKKAAKLFINKKFQQSFEIVQNQLIPQSVVFYNSGVINEDLFNNLWLLYFNLIDIFINKSFTLLSKFDKDQLLKSFKSDEFFNGFYELNKLVHPKLIVLLVLIKLNNDDTDLIALRNQMDIYLVNISSELNHVQNQDRFDSFQELLEIYHVYLLPKLNEFEESEFLIKSNHLIVNQNEMLENLHKVEKEMKDKELQKQKLAAKRAQEAQRRKEQQLKEEQELKRVQNLEKVKNKIIETNSINHNSKIVKKSQGNDLSIYNILRDKLLERFNASNSSIIVLIISLISIIAFTKNHKLLLNNRIKQFLLRFWTKLSNTLKMAFQVTYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3A3</accession>
    <name>A0A1E3P3A3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_104752</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038891.1">
        <property type="nucleotide sequence ID" value="XM_019180427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3A3"/>
    <dbReference type="GeneID" id="30197673"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="671" mass="77897" checksum="44E18E19324654AC" modified="2017-01-18" version="1">MYRNYTKPLTELKTEFVLHNFTQILINIQRELDKPLLEINFNNFYKDLDEVDHYEDYITEVDTLLLRCTIKHLINQLPESAPDHPVCELLDGDFFYNIYKIAFIVPMLNFEDQIRVPRGFHSYDSTNWLSRNLVDVQACLDFTPDLDVRLRGLISKFESKIDHVVSQRRCESTFKTYFWGLTRGMLAQIVSDHTLSRSEIDMRLVLHASLLSPIKMFFNGLFHTNLEVYDDTFGAEMYGIEQLLPEDSPARRLSKIIIKPDLYIVDQTSGEVKTILNLKKPSIDFSEDYQLQDRWDNIDQDINKLLVNLILEKSRIGFITDGYSTMFARFPKNPKVLRSNCHDTLQCIIDSKHLNNRFIDASSSELNISTRLLLAVELLESLSLENDNSDFLNEKQLTKLDDIWRVEISNIVDNLDIGELTTTKHKGFADYFTKFLPWVSQEIGPIDVFIKSVPDHEVIKSAGGNSPIEILCLNSIAFTQCFAPLVVPSSDRVILKIFDLRRMISFYEYHKKKDEIFMMEHNVFIPFQFCQSIIRNYYYNELDCYQKITSYNKREITEETSQPGHEIIKIPELVGYGKLHTREYNGFYIAISDHSQRSPVVKRPRSASEQARYECGLVDGSKSNGLVCHNDLVNSDCWINDGIYSFATIFKKHEDHLEAPDDSDDTLVPTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3F0</accession>
    <name>A0A1E3P3F0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Scaffold protein Nfu/NifU N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_32012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NifU family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038629.1">
        <property type="nucleotide sequence ID" value="XM_019181909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3F0"/>
    <dbReference type="STRING" id="683960.A0A1E3P3F0"/>
    <dbReference type="GeneID" id="30199155"/>
    <dbReference type="OrthoDB" id="565552at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044572">
        <property type="term" value="P:[4Fe-4S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.70">
        <property type="entry name" value="Scaffold protein Nfu/NifU, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014824">
        <property type="entry name" value="Nfu/NifU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036498">
        <property type="entry name" value="Nfu/NifU_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035433">
        <property type="entry name" value="NFU1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001075">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178">
        <property type="entry name" value="IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178:SF1">
        <property type="entry name" value="NFU1 IRON-SULFUR CLUSTER SCAFFOLD HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08712">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01106">
        <property type="entry name" value="NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036773">
        <property type="entry name" value="HIRIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00932">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110836">
        <property type="entry name" value="Hypothetical protein SAV1430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Scaffold protein Nfu/NifU N-terminal" evidence="2">
        <location>
            <begin position="24"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="234" mass="26513" checksum="EC7D5E95FE282956" modified="2017-01-18" version="1">MLTRSTRLLNIKQSTPSLLRYLSIKTLSTPNENALKFQVDQNLLQDNTPSIQISNSNQSISPLASKIFQYAKDVDSILIGSNFITINKDEFAHWNQVTPLVKSILENHLENNEEIITKDQIQQDESTHDEHYATLSEEDQEISDMVAELIETRIRPTIQEDGGDLVFMRYSQGKVYVQLQGACTSCSLSDDTLKSGIQGMLSHYIDGIDEVVNLAEVEFNKFEEKLRANKESRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3S1</accession>
    <name>A0A1E3P3S1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_29803</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the free Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039336.1">
        <property type="nucleotide sequence ID" value="XM_019181652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3S1"/>
    <dbReference type="STRING" id="683960.A0A1E3P3S1"/>
    <dbReference type="GeneID" id="30198898"/>
    <dbReference type="OrthoDB" id="15735at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033745">
        <property type="term" value="F:L-methionine-(R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.40:FF:000008">
        <property type="entry name" value="GAF domain-containing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051330">
        <property type="entry name" value="Phosphatase_reg/MetRdx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021:SF15">
        <property type="entry name" value="FREE METHIONINE-R-SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021">
        <property type="entry name" value="GAF/PUTATIVE CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAF" evidence="2">
        <location>
            <begin position="65"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="176" mass="19092" checksum="78E3E1535AEFA4D7" modified="2017-01-18" version="1">MGEDQHHADYSNFSSSSKKETLEQLVLSYTALSTDLTNWVANLSNASSLIWHAYHSLNLPVNWAGFYVVDPKDKSQLILGPFQGKVACQTIKIGKGVCGTAAGTENTQVVPNVDEFPGHIACDGETQSEIVVPIVGKDGVLRGVIDIDCLIKEGFNQIDQEFLEQLAGLIANGNDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3T0</accession>
    <name>A0A1E3P3T0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_78539</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039117.1">
        <property type="nucleotide sequence ID" value="XM_019184744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3T0"/>
    <dbReference type="GeneID" id="30201990"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133642" description="Secreted protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="16574" checksum="9EEFFD3AEDE6AFE1" modified="2017-01-18" version="1">MFCKQVQFAIFTALFYVMAVAVHPESSVKLHGSFAEYSLSFPGDFLNSTTDTFLFRGYFQGDRTGFEFSTVKASGGPKQKSLEIPKDSDFATFAGAYEFEEPQTDPFNVTVFVRDIFGSAFPTGAKLRLYGAFQEGTNPIQDSFALTVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P405</accession>
    <name>A0A1E3P405_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_92438</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039426.1">
        <property type="nucleotide sequence ID" value="XM_019186362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P405"/>
    <dbReference type="GeneID" id="30203608"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="71" mass="8198" checksum="2394164482136B2D" modified="2017-01-18" version="1">MADFEKLKFLANLAHLTFQKVTGSTKAQVRKVCEFIKEFVHHGLHCCYGFHGLIFFKRLATGSRLHSRQMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P472</accession>
    <name>A0A1E3P472_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">calcium/calmodulin-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_42891</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.17" evidence="2"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039503.1">
        <property type="nucleotide sequence ID" value="XM_019182574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P472"/>
    <dbReference type="STRING" id="683960.A0A1E3P472"/>
    <dbReference type="GeneID" id="30199820"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000449">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000278">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="35"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="344"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="359"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ60296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="420" mass="47143" checksum="EDE12C5DD6051158" modified="2017-01-18" version="1" fragment="single">MPFVQPASEPDGHKFQKFFNKLSGQPPSYAQKTDYIFGRTLGAGTFGIVRQARRISTKEEVAIKIVMKKALKGNEKVFYEELGLLQKCKHPNIVGFRDWFESKDKFYIVTQLATGGELFDRIVKEGKFTESDAVIVIVQILKAVSYLHDELNIVHRDLKPENLIYITEDPNSQLVLADFGIAKELLTPDEVLTSAAGSFGYCAPEVLMGTGHGKPCDIWSLGVIAFTLLVGYSPFRSENVNDFLQEVQNDPVVVFHKQYWKNISQYAKDFIKSCLTVDPKKRPSAKQLLSDPWITANSDVYATDDLAPQIRQFNAKKKFKQAVEIVKLNNRIKKLRELEDDEGEEDSDLALCKQDPDSSSESTSSSERAFDLSSIVQSLKSASSSSSSKSKSELNASLFHQVVRAATNNREKVLSYDEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P488</accession>
    <name>A0A1E3P488_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_78845</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. eL19 may play a role in the last stages of translation initiation, in particular subunit joining and shedding/releasing factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). eL19 lies in close proximity to the binding site for eukaryotic initiation factor eIF4G.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039442.1">
        <property type="nucleotide sequence ID" value="XM_019184795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P488"/>
    <dbReference type="STRING" id="683960.A0A1E3P488"/>
    <dbReference type="GeneID" id="30202041"/>
    <dbReference type="OrthoDB" id="5407653at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01417">
        <property type="entry name" value="Ribosomal_L19e_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.240:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1650.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01475">
        <property type="entry name" value="Ribosomal_eL19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_eL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="Ribosomal_eL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023638">
        <property type="entry name" value="Ribosomal_eL19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_eL19_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_eL19_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033935">
        <property type="entry name" value="Ribosomal_eL19_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057259">
        <property type="entry name" value="Ribosomal_L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057260">
        <property type="entry name" value="Ribosomal_L19e_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006343">
        <property type="entry name" value="PRK08570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25476">
        <property type="entry name" value="Ribosomal_L19e_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="Ribosomal protein L19 (L19e)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00526">
        <property type="entry name" value="RIBOSOMAL_L19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL19" evidence="8">
        <location>
            <begin position="5"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="163"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00065512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="191" mass="22050" checksum="95A5B083D77F8335" modified="2017-01-18" version="1">MNKANLRTQKRLAASVVGVGKRKIWLDPNETSEIAQANSRQAIRKLYKNGTIVKKPVRVHSRASARALAESKKLGRHTGYGKRKGTKEARFPSQVIWIRRLRVLRRLLAKYRDAGKIDKHLYHSLYKAAKGNTFKHKRALVEHIITAKAEAAREKALKDEAEARRLKNRNARERRQQRIAEKREALFKDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4D7</accession>
    <name>A0A1E3P4D7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Inositol-1-monophosphatase</fullName>
            <ecNumber evidence="5">3.1.3.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_100406</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a myo-inositol phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84139"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="5"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039375.1">
        <property type="nucleotide sequence ID" value="XM_019180294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4D7"/>
    <dbReference type="STRING" id="683960.A0A1E3P4D7"/>
    <dbReference type="GeneID" id="30197540"/>
    <dbReference type="OrthoDB" id="10254945at2759"/>
    <dbReference type="UniPathway" id="UPA00823">
        <property type="reaction ID" value="UER00788"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01639">
        <property type="entry name" value="IMPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000004">
        <property type="entry name" value="Inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033942">
        <property type="entry name" value="IMPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854:SF39">
        <property type="entry name" value="PROTEIN QUTG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 4 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="318" mass="35331" checksum="E4FABF57D3B75E38" modified="2017-01-18" version="1">MSIDLKEIYEFAIKVAEEAGSILLKSSHSRTSSDHKLKQNSVDIVTEVDEAVEAFIKKEVLSKYPTHKFVGEESYGKDEPKDRYIIGNEPTWCVDPLDGTVNFVHMFPMVCTSIAFLIDGKPVVGVINAPFLNQTFSAYKGGGAWLNKSTKLPIHDPIPPFPQEAPKGLLFSCEWGKNRDYNGDLKLKIESFINMAKKNEGGGMVHGVRSLGSAALDMAFIAMGSTDIFWELGCWEWDIAAGVILVEESGGLVTNGNPPLDHELSDIGPAHLGSRQYLAIRATTSDNLNETARESQERIVREVWKRVNKIDYKRLTIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4K3</accession>
    <name>A0A1E3P4K3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_79018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039626.1">
        <property type="nucleotide sequence ID" value="XM_019184820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4K3"/>
    <dbReference type="STRING" id="683960.A0A1E3P4K3"/>
    <dbReference type="GeneID" id="30202066"/>
    <dbReference type="OrthoDB" id="46189at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="343" mass="40730" checksum="6D90595168831740" modified="2017-01-18" version="1">MDLLNTTPLQLFQTKSLPEIQDLSSQLSSISIRKHDEIRNLVGSKYRDLLISADEIKQMETLAYKEDKQLYDLVFNAGGKRLDERNVERFFTKEEKKVGGFNHVLENSEFFIKLSKFLKIDQDYSFDKIYEIINELGNNWGDGKDIFVILEKFKQVEIILFDKVDKIDDIEELFKLRSFISGNVNFNLNKFDERLFGKILKMIKIDEFIDLKLILIKFPNFQNELKQTFANEIIDNLETNESLINELHDPKDTLKLYELKFDQDYLTNIDYLSKGLINKQYQDISDFFNKIGKNLNYLKLIINPKSDEFKQFELKYNQVLQKFRNHAEFENNKLLIKYLNKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4M0</accession>
    <name>A0A1E3P4M0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_92857</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039646.1">
        <property type="nucleotide sequence ID" value="XM_019186386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4M0"/>
    <dbReference type="STRING" id="683960.A0A1E3P4M0"/>
    <dbReference type="GeneID" id="30203632"/>
    <dbReference type="OrthoDB" id="416093at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="139" mass="15328" checksum="539ECC3BD1D772C8" modified="2017-01-18" version="1" fragment="single">MNFANTRSFGDVIAKSKGITAEPDITSYIIGDSSEIFKKSLVNQTIGGKGGDECFLVLITDGVTNYANDQEIVDLIKTTHNNKLGKPQDCAEEVIKYVEAIGGDDNATCLVIRLNKWGKWPMEDKTGRIREERLKMGIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4M2</accession>
    <name>A0A1E3P4M2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCAAT-binding factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_92445</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039437.1">
        <property type="nucleotide sequence ID" value="XM_019186363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4M2"/>
    <dbReference type="STRING" id="683960.A0A1E3P4M2"/>
    <dbReference type="GeneID" id="30203609"/>
    <dbReference type="OrthoDB" id="28947at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030690">
        <property type="term" value="C:Noc1p-Noc2p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040155">
        <property type="entry name" value="CEBPZ/Mak21-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12048">
        <property type="entry name" value="CCAAT-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12048:SF0">
        <property type="entry name" value="CCAAT_ENHANCER-BINDING PROTEIN ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCAAT-binding factor" evidence="3">
        <location>
            <begin position="246"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="157"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="358"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="172"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="430" mass="49985" checksum="A596206EF17785D2" modified="2017-01-18" version="1" fragment="single">MLTKKQLALFYFEDYLKQYFFKVIQILERLSHDPIIHVRMNVVSHIFDLLKSKPEQEVNLLRLGCNKIGDIDNKVSSKTSYQILQLEQAHPNMKKIVIDAIVDNVFKVNSDYHTKYYSVLTLNQTILTRREDETANVLVKTYFALFEKLLIESDPNNVDTEPSKDAKANNFKNRKKNFKKGKKGGKSVKNDKSEQEVLEEKNSKLFSALLTGLNRAFPFSNLPTDVFNKHLETLYKITHSSNFNTSVQALVLISQIVKNQNLNSDRYYRTLYESLFDTRLVTSSKQGIYLNLLFKSLKQDANIPRVMAFVKRIVQICYNWINIGSVAGMFYLLMELEKSIPQIRNLAINTPVDHKYVEDDDSEEENFKDVDSENEDSVKKVEPKKKQIAEYDGRKRDPKFANASNSSLWEINEFLNHYHPTVQLYAESFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P529</accession>
    <name>A0A1E3P529_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial nicotinamide adenine dinucleotide transporter 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_51144</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039783.1">
        <property type="nucleotide sequence ID" value="XM_019182661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P529"/>
    <dbReference type="GeneID" id="30199907"/>
    <dbReference type="OrthoDB" id="10266426at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015215">
        <property type="term" value="F:nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000075">
        <property type="entry name" value="Nicotinamide adenine dinucleotide transporter 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044712">
        <property type="entry name" value="SLC25A32-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45683">
        <property type="entry name" value="MITOCHONDRIAL NICOTINAMIDE ADENINE DINUCLEOTIDE TRANSPORTER 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="21"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="124"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="233"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ60576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="36985" checksum="52D57EBB4E36AF58" modified="2017-01-18" version="1">MTPLNTSPDPIRKSTTRSNFTDTQIIALSGALAGFIAGVSVCPLDVAKTRLQAQGLSSNKKYHGITGTLNTILKDEGVKGLYRGIVPIIMGYFPTWMIYFTVYEKAKLYYPKIFQNQFGHNNHNEFFVHSLSAFTAGSVSTSITNPIWVVKTRLMLQTGDESLEKNYYKNTIDAFKKMYKNEGFLVFYSGLIPSLFGLFHVAIHFPVYEKLKKFLKCDNFKSQTNDELQTHSNNLIRLITASSLSKMCASTLTYPHEILRTRMQISSFNKNKSIKTGLINTILNIYKKEGLIGFYQGFTTNLARTVPASAVTLVSFEYISRYLTTALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P548</accession>
    <name>A0A1E3P548_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RecA family profile 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_61164</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P548"/>
    <dbReference type="STRING" id="683960.A0A1E3P548"/>
    <dbReference type="GeneID" id="30200366"/>
    <dbReference type="OrthoDB" id="3981235at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033063">
        <property type="term" value="C:Rad51B-Rad51C-Rad51D-XRCC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033065">
        <property type="term" value="C:Rad51C-XRCC3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000707">
        <property type="term" value="P:meiotic DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052093">
        <property type="entry name" value="HR_Repair_Mediator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46239:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46239">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 3 RAD51C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="8">
        <location>
            <begin position="28"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="353"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="419"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="491"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="374"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="419"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="491"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="549" mass="62255" checksum="7E6EFFF8C2B41BF8" modified="2017-01-18" version="1">MSSIPTPSQTTIHRLPPQSLSQLIGTSSTPPISTSIKYFDEALDGGFQSGSLYEISAINGSGKSSLAAQLTYNCLQMGKHVLWISTLQSIPLQKLRDMCEQELLGNLHHIRLNTIGSLLLFLQSLPLKKTKYSLIIIDDFMSCITRCFDEHEIMINPQHLKSKQNALESKKNRSIQTLLKLISTYCATNDASCVLFNSSTILNMSFVERKKHESSQVPSSSAQFSSQNPSQKPKRFYQQILVSPMGDHPFWSQYFKARMMLYRDWLSSSSSSTTAIFVHLKFNSLVSKIHTPKVISFQESSKGLVEVSKDLNLLISNSSNSFSLEEEDFEDSENEETEIRTTAMNEHPVLLMNSSPRHKGISTAKDILPDDLTNEVSSQMASQTTRTTEIEKSIEDNQNEQDDDEENDSSVIQLNENQFENSDIQSTPEPNIENQSEEVNNENWDKENDNLQFSVPMVEDTTVEIEEIEDELEELYPTQPESLSFIQLSQIHNSQSQTSKRSFPSPPSKTRSVKKLRHPLQDFTNEYHHESYQTQLSSDEYIPASAPYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5C9</accession>
    <name>A0A1E3P5C9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA mismatch repair protein S5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_61246</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039886.1">
        <property type="nucleotide sequence ID" value="XM_019183141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5C9"/>
    <dbReference type="STRING" id="683960.A0A1E3P5C9"/>
    <dbReference type="GeneID" id="30200387"/>
    <dbReference type="OrthoDB" id="10263226at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032389">
        <property type="term" value="C:MutLalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061982">
        <property type="term" value="P:meiosis I cell cycle process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000017">
        <property type="entry name" value="PMS1 homolog 1, mismatch repair system component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="MutL/Mlh/PMS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF44">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein S5" evidence="4">
        <location>
            <begin position="227"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="433"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="433"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="456"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="619" mass="71186" checksum="3A385A43AAAD9AD1" modified="2017-01-18" version="1">MINKIPLESVASITSTSSIFSPVSAIKELIDNSVDANANSISIDLDTKTCGLQYIQLKDNGDGIPKDDRHFLFLNHTTSKISKAEDIFTVNTLGFRGEALYFINQLTFPNGSIEISTKTSIDKLGESWKIGKDGELLNSSLKKISIPNGTIIKIQNIFKKIPVRYKILIKNSKFHKDKIKQLIWQYSLIHPTIKFHLKFINFTPKDRQSSSISSQEIYHSKNPLQSISKHFNQLKFFHNLTIINNWEISIILPKMDTGHHTNLPLKLISINKRLITLSMFKKLNKILNNVYESLKLFKPLVWVIQLTNHNDKIDVNIEPEKNDVLIINELDFLESLENFLLKCVVEGHGLIENVDESGKESDEDEINKEKMVETDNLINKEKEVEAENIDQIEVQNHDNMRDQYVEINKVGINPNHNSLDITNDTIDWSHNMHDNLSSRSNTRTNTIESLPKSDNQEEEEEEEEDEESFKIAKDTTISNPFTLTNLRYNSSNKSSINHLPTPDNSIDEIKNNKPTFKLPNPPKLLQTKTTHPVTQQILQTKRYKLSSFQYLQSFHLKIIPKLDEFILNEDYQINSNNDISSNILNFVKQHESTNFEILIDEAGLLKIKPKELQLTETKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5W4</accession>
    <name>A0A1E3P5W4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Checkpoint protein RAD24-like helical bundle domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_15884</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad17/RAD24 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040038.1">
        <property type="nucleotide sequence ID" value="XM_019180663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5W4"/>
    <dbReference type="STRING" id="683960.A0A1E3P5W4"/>
    <dbReference type="GeneID" id="30197909"/>
    <dbReference type="OrthoDB" id="10265971at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004582">
        <property type="entry name" value="Checkpoint_prot_Rad17_Rad24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057927">
        <property type="entry name" value="RAD24-like_helical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12172">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12172:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03215">
        <property type="entry name" value="Rad17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25812">
        <property type="entry name" value="RAD24_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Checkpoint protein RAD24-like helical bundle" evidence="9">
        <location>
            <begin position="321"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="520"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="520"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="535"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ60831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="563" mass="63547" checksum="8FD4F1485C93460F" modified="2017-01-18" version="1" fragment="single">VISDDEDSEELNPLPEVKKQKLSSLRGHIKSFSSNTPQSTRSPTPELPKAPSNLDQFDQWIDKYSPKSTSEVAIHSKKLAEVKEDLELMISGDSPYRILLLSGPAGSSKSTVAKCLGRELIPAIRHTQPSETDIIEFTNDDSIESTVTQFSDFLDSVKYRTGKNLSMVLVEDLPNVFHGPTRDNFQQALQQWLYKDLFLPPLVLCVTECEFPNDGPTSQGFNIDNNFIVETILGRKLLMNPQIKRIKFNPVNMTLTRKCLTNIVSQEGDLFNNIPRQEILDKIKELSDNGDIRSSIAAFQFWASYRSKHDDSRKFISIGKEPSISLFHAIGKCIFGTKNEEEDDITTLDKVLNDFISKPNILKLGILENYTKFNKANYDLESACDIAENLSVSDLFGNDDSSIEFAARSTRTVFRNVNVKSSSNGSNHSGATFPREWKVQRGINATKSDILRYIETELKRRQAYRTFQDSNLLYGYYEPIINKQRGSNPFISERLGGPFKEVFGDSDIVPDEEITRDRYFKTSNIKDDDSSSFDSSDDSELENDPIVDTDADDEAGDEFGDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6L4</accession>
    <name>A0A1E3P6L4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_27987</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040190.1">
        <property type="nucleotide sequence ID" value="XM_019181435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6L4"/>
    <dbReference type="GeneID" id="30198681"/>
    <dbReference type="OrthoDB" id="4139357at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000171">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF4">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="432"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="466"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="494"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="60"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="523" mass="57848" checksum="5DB25BAD011DB228" modified="2017-01-18" version="1">MSSAKAEKTSKSLSDEESLTSGRFSSFFIKKGDLSNFDTADDAFRSKINLINDALDEIGFTWYHFKLFCLNGMGYAVDSDLTFLQSSVAQYVRYQYRQGFPADSNAFFAGLFFGALFWGFGADLIGRKVAFNSSLFMSSIFAILTGCMGNFGTYCLFVALTAAGAGGNLVMDTTIFLEFLPHKNQWLVTLMAAWWSIGQTVAVLTAWLFFSQDKFICHSEDDCSDASNRGWRYVWFVNGGLVLIIAILRLTIIELQETPKFLVANNRDREAIETLQTIANKYNRTCSLTLKELEAFGPITSNEDYLQESSFKTTFRLIWKHIKTLFSNRVLALSSTLIFLSWTLLGIAYPLYTNFLPIYLATRGPNISPQTVSGVYRDAVISNASSMGGGLIGGFLLYYCPVLGRRGVMVIGGLSSMALFFGYTQIKTRQQNVGLSSAVYVAIYIYFSCLYAYTPEIMPSNARATGNALAISCMRGMSCIVPVIAYFADTGTSVPIWISGACIGIIGICSIFLPFEPSKQRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P700</accession>
    <name>A0A1E3P700_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH CENPB-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_28821</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039939.1">
        <property type="nucleotide sequence ID" value="XM_019181540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P700"/>
    <dbReference type="STRING" id="683960.A0A1E3P700"/>
    <dbReference type="GeneID" id="30198786"/>
    <dbReference type="OrthoDB" id="125347at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050863">
        <property type="entry name" value="CenT-Element_Derived"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004875">
        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041188">
        <property type="entry name" value="HTH_ABP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006600">
        <property type="entry name" value="HTH_CenpB_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303:SF73">
        <property type="entry name" value="PROTEIN PDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303">
        <property type="entry name" value="TRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03184">
        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18107">
        <property type="entry name" value="HTH_ABP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03221">
        <property type="entry name" value="HTH_Tnp_Tc5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00674">
        <property type="entry name" value="CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51253">
        <property type="entry name" value="HTH_CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH CENPB-type" evidence="2">
        <location>
            <begin position="64"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="445" mass="51790" checksum="3FD9BA7B9C820299" modified="2017-01-18" version="1" fragment="single">MGYSIKQKIDICLMAESNPDMTQADLATWAKKHYGSSKPPSQTTISRILSKKDELIALKEHEFKLIRRRRLSNPLLRKVLLEWISQCVWNNIPITAPIIMSSASNFWNHLPDNLREGSGEFSYKWCSQFLSKVNINLNNIEKELYKRLKIWTFEERSILQNLLTGVDLKKVFTLDEIFLSYDLPLDKSCYDDTSDFLTCMLCVNADGSEKLDPLIIGRYENYPSFENKSSIKAATKHGVSYHSNRMKWLTSTVFYDWLAVLDKRLALQGKDIIIILNDSASHRVINIKLQRIRLLFTSSSSNFLPMNWGIANDFRLTFRIVQYKRLLEKQKKKKGQLLTKEEMRFSIVDVFDLIKKSWDVIPNSRIKSAWKQSGILPKSATDTFERQGMFDDSLEIQLFHLINQLNVKEQWDVLSLLDLTVEQKINKSFLSNEEIIQSCVVDNYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P724</accession>
    <name>A0A1E3P724_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine-threonine protein kinase 19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_77872</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STK19 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040436.1">
        <property type="nucleotide sequence ID" value="XM_019184627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P724"/>
    <dbReference type="GeneID" id="30201873"/>
    <dbReference type="OrthoDB" id="3980126at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046579">
        <property type="term" value="P:positive regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018865">
        <property type="entry name" value="STK19-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15243">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15243:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10494">
        <property type="entry name" value="Stk19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00093458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="34744" checksum="8988608808A1BA98" modified="2017-01-18" version="1">MSLRYNPKKPSILKKDLPKKTRTPKEVEKLQPISQNNIRLDDDIDPKTLLDAIDKVLSRQWNLVPSQLNPDFQAHIQKYRDFLPKLVSRYQLYSIFPNNQTFIDQEIDQLFEDQRIKRILLQDNDDLNDLIMKNEDYRAVLNEHSKKSNDINVQTSIENFINYLDENPKSLKIPKDLIIKTLGDESVSILIKLGFLQIIPQSTTKTLLKISIPKYGYIINLYQTSRKFIVKTINTNKWREIKHNDLYEKWEKNKLKFKDFKGLNLNWILHWLIGEGTLEVFEIGDGKAYRIISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P761</accession>
    <name>A0A1E3P761_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SNF5-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_29514</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040478.1">
        <property type="nucleotide sequence ID" value="XM_019181619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P761"/>
    <dbReference type="STRING" id="683960.A0A1E3P761"/>
    <dbReference type="GeneID" id="30198865"/>
    <dbReference type="OrthoDB" id="515064at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006939">
        <property type="entry name" value="SNF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10019">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04855">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="370"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="370"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="383"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="408"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="56693" checksum="97FB85FE7FED5CA7" modified="2017-01-18" version="1">MQQRKPSIIENKQPGSTPTQPPQQQPSNQFLLAAQTITKVQENTHYWSEKLKQESKLLTKDVLLYEQLINRDITNREILSKEKEQGSNKQLLARLVGDLKFYNDLKVSRMKTIGLSNAGHYTNTIWGEGYQGYGNGFSDGQTRVILPKDRKKPSRLRDFQISKKLNEIQAYQSDDFIPIRLEFEIEKDKFKLRDTLLWNQNEKTITLENLVALIMEDYKLNNPLLSDTILASIKEQVTEYHQHVFNNKFGYDLRILIKLDIIIGNNQLIDQFEWDISNPLNSPEEFAEAMTSELALPGEFTTAIAHSIREQAQLYTRALYLIGYDFQGGYIDEDEIRTNLRTIVTHQDYLRARTQVTQFTPQILEVSNPELERLDKDRERDSRRKRRQGRTGRRGGPVLPDLKDVPRTFRTPVPTTTLPGGVDFTDSVNSYHVITETINKPVESKATPRAPVNQGSVHSTPVIVHETMGIPGRKRKVVVSHVPGVSCIVNIKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7A6</accession>
    <name>A0A1E3P7A6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DDHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_29417</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040513.1">
        <property type="nucleotide sequence ID" value="XM_019181602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7A6"/>
    <dbReference type="STRING" id="683960.A0A1E3P7A6"/>
    <dbReference type="GeneID" id="30198848"/>
    <dbReference type="OrthoDB" id="69269at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008970">
        <property type="term" value="F:phospholipase A1 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032048">
        <property type="term" value="P:cardiolipin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046337">
        <property type="term" value="P:phosphatidylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004177">
        <property type="entry name" value="DDHD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058055">
        <property type="entry name" value="PA-PLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055555">
        <property type="entry name" value="PA-PLA1_DUF7131"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057826">
        <property type="entry name" value="WWE_C20G8.02"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509:SF10">
        <property type="entry name" value="LD21067P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509">
        <property type="entry name" value="PA-PL1 PHOSPHOLIPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02862">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23465">
        <property type="entry name" value="DUF7131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23463">
        <property type="entry name" value="WWE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01127">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51043">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDHD" evidence="2">
        <location>
            <begin position="535"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="729" mass="83984" checksum="177CB6053EF222A7" modified="2017-01-18" version="1">MLFRSLVIDQSAILKRIPHVSTSTTTTSKRHFTFIKKKNFPGLGNIKEEEQNQSQISTKDEKLQPYKPKIPPLKPRWFYATDNPISMPKEYEYVKKEDPKKFSAFSEFDSERIEKKYQHLAKQLHNDGELKDTDKYKVPVNEDYLFEVDLLEKKMYPVYWEGAVYEIRRGLWFNSDGYPLREELSEELEKGYAKLKPFAKKIKNNKEKDDDAEVNLENEHGQRDIYKLDGFKEGKLVVYSEDKTAYILNELYGGKLQVGYLKGLQNGPALGASRVTRGFSGSSDTETKPERSSLSSIFELELSQMFGEKTKTNTAKSDHENTKQAMEIEIEQDYDNEEVEDGTTSKGREVDHLIFCIHGIGQTLGTKFQGVNFIHTVNVLRKNIKKVYEQNKDFQKLLNNDKNCRIQVLPISWRHKIDFSTHKPSEDRDEHGNHRLPTLNDITVEEMKPLRNLLGSVLLDVLLYYEPRYLNQILDEVTTQANDLYHLFKKRNPSFNGKISIIGHSLGSAIGFDILSMQPKQPIPKDQQDKPSRYLDFKVDNYFALGSPVGVFNLLKLKNIGSTELNGSSNDKMSVPRCDNLYNVFHPCDPVGYRIEPLISPEFKNFQPESVPFLIEDFNKQLASWAELSESLIPDALKDDEQDKSVQLKQAKLTDEQLAKLSSLNYNGRVDYELKQGYFDLTIISSISAHVSYFEDENIAGFILKEVLAKHEKVKEKTAKVRVEKKQSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7C3</accession>
    <name>A0A1E3P7C3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Guanylate kinase</fullName>
            <ecNumber evidence="2">2.7.4.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">GMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_77949</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the guanylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.8" evidence="2"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040522.1">
        <property type="nucleotide sequence ID" value="XM_019184639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7C3"/>
    <dbReference type="STRING" id="683960.A0A1E3P7C3"/>
    <dbReference type="GeneID" id="30201885"/>
    <dbReference type="OrthoDB" id="6334211at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:GMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000002">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000776">
        <property type="entry name" value="Guanylate kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00328">
        <property type="entry name" value="Guanylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017665">
        <property type="entry name" value="Guanylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03263">
        <property type="entry name" value="guanyl_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117:SF13">
        <property type="entry name" value="GUANYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117">
        <property type="entry name" value="GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="9">
        <location>
            <begin position="3"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ61315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="186" mass="20787" checksum="9FE3A1EBB2CDCCAE" modified="2017-01-18" version="1">MSNRPIVISGPSGTGKSTLLKKLFAEFPDKFGFSVSNTTRQPRQGEVDGKDYHFTAVDDFKKLIDEKAFIEWAQFSGNYYGTTIKAVEDVAKLGRACILDIDMQGVKSVKNTNLNARFLFLAPPSIEILRKRLEGRGTETQESVEKRIAAASAEIEYSKTGAHDKIIVNDDLDKAYSELKEFILQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7F4</accession>
    <name>A0A1E3P7F4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_61912</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the CENP-W/WIP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040563.1">
        <property type="nucleotide sequence ID" value="XM_019183272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7F4"/>
    <dbReference type="GeneID" id="30200518"/>
    <dbReference type="OrthoDB" id="2543597at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13732">
        <property type="entry name" value="HFD_CENP-W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028847">
        <property type="entry name" value="CENP-W"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052484">
        <property type="entry name" value="CENP-W/WIP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34832">
        <property type="entry name" value="CENTROMERE PROTEIN W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34832:SF1">
        <property type="entry name" value="CENTROMERE PROTEIN W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15510">
        <property type="entry name" value="CENP-W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="8600" checksum="D4EF2B7268F1F4D3" modified="2017-01-18" version="1">MAPITRSSIRRIIKSKTDLKLANDSTDILINLDYMLFVRDLMFEAEKLADKSGSSEILSTHLRMARVNVLKKYRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7J7</accession>
    <name>A0A1E3P7J7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_83444</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.n1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the GTP-binding elongation factor family. LepA subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040467.1">
        <property type="nucleotide sequence ID" value="XM_019185546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7J7"/>
    <dbReference type="STRING" id="683960.A0A1E3P7J7"/>
    <dbReference type="GeneID" id="30202792"/>
    <dbReference type="OrthoDB" id="1074at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097177">
        <property type="term" value="F:mitochondrial ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03699">
        <property type="entry name" value="EF4_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01890">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03709">
        <property type="entry name" value="lepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000078">
        <property type="entry name" value="Elongation factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000015">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000007">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2570:FF:000001">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000004">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Elongation factor 4, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00071">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006297">
        <property type="entry name" value="EF-4"/>
    </dbReference>
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        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
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        <property type="entry name" value="EFTu-like_2"/>
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        <property type="entry name" value="G_TR_CS"/>
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        <property type="entry name" value="LepA_C_sf"/>
    </dbReference>
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        <property type="entry name" value="LepA_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035654">
        <property type="entry name" value="LepA_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01393">
        <property type="entry name" value="lepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512:SF7">
        <property type="entry name" value="TRANSLATION FACTOR GUF1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512">
        <property type="entry name" value="TRANSLATION FACTOR GUF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06421">
        <property type="entry name" value="LepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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        <property type="entry name" value="G_TR_1"/>
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    </dbReference>
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        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="43"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="52"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="115"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="169"/>
            <end position="172"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ61260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="636" mass="71195" checksum="097FA3726926E7D6" modified="2017-01-18" version="1">MLGSRLSCINTFIKPLTIRRGFTSSSKLFEKSIQERINDIPIENYRNFSIVAHVDHGKSTLSDRLLEMTGVVQPGQKQVLDKLDVERERGITVKAQTCSMFYNHKGQDYLLHLVDTPGHVDFRAEVSRSYASCGGALLLVDASQGVQAQTVANFYLAYSMNLKLIPVINKIDLDMADIPRSIDQVVNTFELPEEEIIQVSAKTGLNVDQVIPAIIERIPPPVGKVELPLRALLVDSWYDSYLGVICLVHVVDGTIKKGQKVFSPHTDKKYDIKETGIMYPDRIPTNTLSAGQVGYIVPGMKDSSEAMIGDTFMNVAEPVEPLPGFEEPKPMVFVGAFPADMTEFSAFDEHMQHLVLNDRSVTLQRESSNALGQGWRLGFLGSLHASVFKERLENEYGASLILTSPTVPYKVLYSNGTEEIVTNPDDFPDVISKRHQVSKLQEPYVEAIMTFPQDYLGSVIKLCENNRGIQKEISYLTTGQVLLRYEVPLSHLVDDFFGKLKSATRGYASLDYEDSGYQDSDIVKLELLVNGNGVDALAQVMHRSQVDAVGRAWCKKFKEHVKNQLFEVVIQAKAAGKIVARETIKAKRKDVLQKLHASDISRRKKLLVRQKEGKKKLRAIGNVSINQDAYQAFLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7J9</accession>
    <name>A0A1E3P7J9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_28286</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019181468.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="30198714"/>
    <dbReference type="OrthoDB" id="6509908at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032218">
        <property type="term" value="P:riboflavin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF177">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER MCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="320"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="1"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="420" mass="45784" checksum="A8826DC521C998D1" modified="2017-01-18" version="1">MFANDSIDDFPEGGLKAYLAVLGCCFGLVPCFGILNSLGAIETYISENQLANVESSTVSWIFSIFLFVNFTSCIFSGTFFDRNGAKIPLIVGATLLCGGLFATANCSKVWQYVLAFGVLCGLGNGLTLSPLVSVVPHYFNRKRGFFSSIASTGGSIGGIVFPIMLRKLFDQVGYAWTIRIFSFVVMACHIVAIVFARERLPHVKTDRTTTQKLMSYTKAFDISSFKEWKFVFVVLGCTFAELSITTTSTFYTSYTRAQGASMSTAYLLTTVVNIAGIPGRWLTGYLADRVGRFNVIITILLFAGIITLIILIPFGQHEGALYAFSTVWGFSTGSVFSLLPVCCGQISKTEDFGKRYSTMYFVVAFGTLASIPIGGAIIDDRSPVHFNNFMIYSAITAIAAAGFYTVARILCVGSKFTKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7V5</accession>
    <name>A0A1E3P7V5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rad21/Rec8-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_83240</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7V5"/>
    <dbReference type="STRING" id="683960.A0A1E3P7V5"/>
    <dbReference type="GeneID" id="30202754"/>
    <dbReference type="OrthoDB" id="3980878at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006910">
        <property type="entry name" value="Rad21_Rec8_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04825">
        <property type="entry name" value="Rad21_Rec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="532" mass="61115" checksum="FE85296159E7D449" modified="2017-01-18" version="1">MALRLTSNLLYGVALIYKQKTDYLNNDTSLIKTKIQRDLFNQKGSNSDILIKTPNYIIKPIELTRTQQGGNHNNQVLLNDDPLFNLEGDLLPSLGDLDFLQGTSSTQMVNSNHRKSQIRRSDLENNTVSVVLSTMTNSTEDADIFTRHVNQDDILDTQNPEFAFDEEGMLFSLHDETTAGTGGNAGPSEDLHINDGFDIDFDFDIDLERQEAPAQQQPPELDQILEEVEDPPPQQAPDSEQPIVNESPEAVQQPRKKRRKAQNHFTTLVVDETISLQTNDLRGFRDTYIELMDQQRTKPPEPRIAIQNLLDEVSYLPNFGKNPADEERRGRNPVREFEDEDFVDGLLRNTRRSSESIEVRRNASSSRRHSTRASMSSINLPLEGDLQHPQVDDTTGGGGNDNFDFDFDLEFDIDEERVPRKRSSSVQRFPALAEDEEHNYQNNEEMYDDERPEVDRKTIKFLTFIETRLEEDSVQEMKFDQLMQHQQNRSVIVKSFYELLQLSTLNSIEIVENNDKEKFELLDANDFSIRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8A4</accession>
    <name>A0A1E3P8A4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_75750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040747.1">
        <property type="nucleotide sequence ID" value="XM_019184251.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8A4"/>
    <dbReference type="GeneID" id="30201497"/>
    <dbReference type="OrthoDB" id="435881at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="407" mass="47792" checksum="70910040B50AD5B2" modified="2017-01-18" version="1">MVQNIDTRLHSIAADSSSLDPILFAKRNFPQILVAKEQDEVLLHNIIAAVSKSFKAYSFFLRKEDVDQFYENFKQETLEPTHFKDSLIMLLLLSICIRLKKFQCVHIPFDKKEKLVEEIFQEYKGRCHLEVLEKNYKCLQIGVLELEFMIGMDQYREDILVGVHEVVGIGKFIKWRDGKTNPELEACWLELQRMDAIIAIFFETRPCITDPVNKDLSVYKSDDRRCKMAPIYINTIQILQKYNNSGLKDISHQEINDQIDLLYTVQDLFFLLDAACLYHIKLLLHYNESDFAGYTKTLELFLVRIWKVSLADDSHITRIKWGLDLIFRTLLKFLLHYKEVNGELTISLSIIICLNNLFSFGSHDQEDIAHKLESYCPKLNFEYCFELITNFEKENPHLFPECKTMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8A7</accession>
    <name>A0A1E3P8A7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Intimal thickness related receptor IRP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_59728</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040852.1">
        <property type="nucleotide sequence ID" value="XM_019182864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8A7"/>
    <dbReference type="GeneID" id="30200110"/>
    <dbReference type="OrthoDB" id="10512565at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133753" description="Intimal thickness related receptor IRP domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="372"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="49090" checksum="61DC478E51CD3DF9" modified="2017-01-18" version="1">MRIISILPLFLLGSLINCHQLTLNEYTSERCSFVNKSIQVQVHSTHDDISIRYLIFHYKDILISDIPNYKNHDKDAPKRCGDDGCGGGYGYDVHFEPDQQHDPVSNGVLSKEKGNLLYDVSNPGFYCIYLDPNADYYGDVIFENNFGHLDVDNYTRLSFKRTFYLPLLTVILLFTFLWKYPLKFKRFVGAVWLIQVIESVSLGLVSKYGLNIWTEVFDALFGDVAVNLIIGFILLQIAYHSFGYEIVDDESMIFKDTKVIKLIILAFVVLKCLNGLFFNDSLIPYYDLHFNSIRRYLLLLQYIPYILVYVSIVFEFLSLQKNFKIDDSRIVKSFQLFTIIPPFIAGLKLFFGAFVIHKSNDPGFYDAYFKDILVLIPAFITDILVLLSILVLCFLWRQRNSSSYERVGNSNADQAIELDDMNTNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8F1</accession>
    <name>A0A1E3P8F1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Delta(14)-sterol reductase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">C-14 sterol reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Sterol C14-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_86590</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP(+) = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17813"/>
            <dbReference type="ChEBI" id="CHEBI:18364"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.70"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:18563"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="15">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 2/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the ERG4/ERG24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040888.1">
        <property type="nucleotide sequence ID" value="XM_019186054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8F1"/>
    <dbReference type="STRING" id="683960.A0A1E3P8F1"/>
    <dbReference type="GeneID" id="30203300"/>
    <dbReference type="OrthoDB" id="10262235at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050613">
        <property type="term" value="F:Delta14-sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000009">
        <property type="entry name" value="C-14 sterol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001171">
        <property type="entry name" value="ERG24_DHCR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018083">
        <property type="entry name" value="Sterol_reductase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257:SF52">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE TM7SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01222">
        <property type="entry name" value="ERG4_ERG24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01018">
        <property type="entry name" value="STEROL_REDUCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="8 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="13 16" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="9 16" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="12 16" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="157"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU369120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ61681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="217" mass="25364" checksum="2A35CE5D03B4C122" modified="2017-01-18" version="1" fragment="single">MLLWLILNLSALHHQWLKLGYVTDSLVLVNILQAIYVFDGVLNEEGVLTMMDITTDGFGFMLSFGDLALVPFSYSLQARYLAVEPIHLGEIYTTIVLLISFTGFYIFKASNNEKSNFRQGKLNHLKSIQTERSTKLLCDGWWGLSQHINYFGDWLHALSWCLATGFKTPLTYYYVLYFASLLLHRQRRDEIKCREKYGEAWKQYEKQVPYKIIPYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8V2</accession>
    <name>A0A1E3P8V2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_82025</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041053.1">
        <property type="nucleotide sequence ID" value="XM_019185321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8V2"/>
    <dbReference type="STRING" id="683960.A0A1E3P8V2"/>
    <dbReference type="GeneID" id="30202567"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03048">
        <property type="entry name" value="GST_N_Ure2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF8">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE GSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="19"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="78"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="23050" checksum="A8BFD0E7E28049B8" modified="2017-01-18" version="1">MSSKNLDLSDPEHPKDLKPPYLKLYSLGSPNGQKISLYLKLLGIEDYYYRQLDIRTNIQKEPWFIAINPNGRVPTLSDVDSQGKQTILFETGAILLYLVASHAPYQGQATQFQHYAPEKIEYGIKRYKGETESVFGVYELRLKENDGWLVGDHFNIADIAAFPWVRVYAYNDIDIDQFPHLKAWIEKIKIIDGVQAGLDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8Y3</accession>
    <name>A0A1E3P8Y3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_59953</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041083.1">
        <property type="nucleotide sequence ID" value="XM_019182907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8Y3"/>
    <dbReference type="GeneID" id="30200153"/>
    <dbReference type="OrthoDB" id="4095569at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="395" mass="44520" checksum="63BC344FFB5F5A1B" modified="2017-01-18" version="1">MQLLRRNRRNSSNSRQNTNSRTATSNAEQQSSTPRSTLNSPIPPEMLRSLSEETLITSGHSIPTTPTHPFSSIPNHIRRTISNDSNLTYISRAETFVDLVLGHSFAEFNAIRTMPVVSALFQKGLYVFPSEESLNLYKEKNRNVDLSLRQKGLGTPILQVTTPMLSAFKKNCPTVVIYRFKTPPINCLNVSPEATIENPKIEFCRVYFKYLKDKIAKYTFQFMPNGDPNDAENETLTMYVSSVTPYADVCYKNTRLRWVGTTSMSSTFGSGFFKLLKLSDESPSLTDHLDENFPDPTNPLLASRDALANTSPLPPLARFSDKQSSSIPKKRLLRHGDIKLIEVFNSGNKDSILDIPYCSLVISSLALVLRDQEIKKYHGNARYYDTSLATTNVFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P935</accession>
    <name>A0A1E3P935_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translocation protein SEC66</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_59895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ61820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019182898.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="30200144"/>
    <dbReference type="OrthoDB" id="73168at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031207">
        <property type="term" value="C:Sec62/Sec63 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="entry name" value="TRANSLOCATION PROTEIN SEC66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28229:SF1">
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="22699" checksum="7153CDE129141F8E" modified="2017-01-18" version="1">MAEEEPVIEISKISVFTPIIYVTVMITALVIFSINYRKKKLNALYSRKPFFNRNIPKELYFQLREQDPKPNEKVLKAALMRRGAESIRRTIKLKEVQPFITALYQKGSIGDDLWERYQAATKLEELEVQELVQEAEKYKKGWIPKFVPLLQEICFNEALRRRYNAIEDRKLALLDHWGIEVQSDGTLVLPNRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9C6</accession>
    <name>A0A1E3P9C6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_60063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ61991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019182932.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="30200178"/>
    <dbReference type="OrthoDB" id="3981234at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="78"/>
        </location>
    </feature>
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            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="36708" checksum="C6D7EB408A6FE02F" modified="2017-01-18" version="1">MEIIMPVGGSGYNFNNNLYSSQAITEHNSSSTTDSNSSAFDFNNYSISYPSSSSSHSQIHQADHQRLGQSTSMPNQSNRSKENLDYPTNEQTNSNFTSSVQFNQPRSGPLSNSQVEYSSYNFQFPSNSYQSNDYSNYSQQQQQQQQQHYQQPSQPLRSPLPPAQYSQVQTPTNVLPQNQYNSNFLYSSSHDQESSNDHPPLYPPRNYQQQHQTSYFPQYSEKSKSFTFNDPFANRSRPDGKLEEFTDDDVQILKSLLFNGEKIKWKYVSSKLANVTGRRATSSACSKKTRELFRLPSEKDCGDLGTSLPYVIHNSWKSLEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9G6</accession>
    <name>A0A1E3P9G6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor IIF subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">TFIIF medium subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">TFIIF-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_76211</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIF beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041238.1">
        <property type="nucleotide sequence ID" value="XM_019184340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9G6"/>
    <dbReference type="STRING" id="683960.A0A1E3P9G6"/>
    <dbReference type="GeneID" id="30201586"/>
    <dbReference type="OrthoDB" id="26094at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001174">
        <property type="term" value="P:transcriptional start site selection at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07980">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000035">
        <property type="entry name" value="General transcription factor IIF subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003196">
        <property type="entry name" value="TFIIF_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040450">
        <property type="entry name" value="TFIIF_beta_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040504">
        <property type="entry name" value="TFIIF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02270">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17683">
        <property type="entry name" value="TFIIF_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIF beta subunit N-terminal" evidence="12">
        <location>
            <begin position="71"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIF beta subunit HTH" evidence="11">
        <location>
            <begin position="317"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="147"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="388"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="181"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="399"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00081473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ62031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="418" mass="47673" checksum="0F2E0FBF0C0150E2" modified="2017-01-18" version="1">MTSEKAQQKATEDGGAEAGPSVSKEQTILQMQQENEGSDEEYISENEQIIDSNDASTLTSALDLNMSNADQHIWLVRLPRFLVEKWKQKEFYQGQELGRIKIKKDSKLADVKLELNNDPSTEDIPHNYDINMLRSAVENQYVFTEQNLDNFNKNGNGDTKKEDDSDEDIIKTPAPTTADGSNSNANTTPAAAAAPKPELTRREAAFLRRRRRFNRFKNQDGTDKVIPFVKTIPKKTALRGTIDHECQVNPLISDPNYSKIIARRKNLVKEEPRPSVTLLNEIPGVTLSNAGLTLKSDTSKFLKSSTSTKNKGEGRAIRMPQKDLFDLLFKLFDEYDYWSLKGLKERTKQPEVYLKETLEQIAQLIKKGPYALKYALKREFKELKDQERAERLGELSKDNEDEDEDDNEEDVEMEDVNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9H6</accession>
    <name>A0A1E3P9H6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hydantoinase/oxoprolinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_82179</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041270.1">
        <property type="nucleotide sequence ID" value="XM_019185343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9H6"/>
    <dbReference type="STRING" id="683960.A0A1E3P9H6"/>
    <dbReference type="GeneID" id="30202589"/>
    <dbReference type="OrthoDB" id="5404895at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1610.10:FF:000001">
        <property type="entry name" value="Hydantoinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.390.10">
        <property type="entry name" value="CV3147-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1610.10">
        <property type="entry name" value="CV3147-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048350">
        <property type="entry name" value="S-Me-THD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024071">
        <property type="entry name" value="S-Me-THD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010318">
        <property type="entry name" value="S-Me-THD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027479">
        <property type="entry name" value="S-Me-THD_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF10">
        <property type="entry name" value="HYDANTOINASE_OXOPROLINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20906">
        <property type="entry name" value="S-Me-THD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06032">
        <property type="entry name" value="S-Me-THD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160991">
        <property type="entry name" value="CV3147-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="1">
        <location>
            <begin position="207"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="S-Me-THD N-terminal" evidence="3">
        <location>
            <begin position="586"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="domain" description="S-Me-THD-like C-terminal" evidence="4">
        <location>
            <begin position="750"/>
            <end position="952"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="953" mass="102930" checksum="59EDAF65EDEE2AB4" modified="2017-01-18" version="1">MQESRLRIGIDVGGTNTDCVLLDPTKQHTESRGIIACAKSLTTPDITTGIFTVMKKVIEASQVKTDKIAIVTIGTTHFLNAIIERDVSRLDKVAIIRLSSNFTRSIPPFSDFPQDLKDAIYGYHGFVSGGLQIDGSLISEINEEEIIQQCEEIKKRKLISVVIAGVYSPLDTKYNQEYKAREIVEKHLPGVRIVCSRDVGTIGFLERENASILNASILRFSEYVIQSFKSALENLGLKCPLYLTQNDGTIIAAKQVKKIPIVTFSSGPTNSILGAAYLSQVKHSKSSILVADVGGTTTDIGVLLPSGFPKQASAYLSIAGVRVNYAMPHVHTIGLGGGSIVAEQADGSFTVGPESVGLSLTKNALVFGGSTTTATDVALLAKHIEIKGGDMSKLTCLSENTIDKSFKQIQLNLSKAIDAIRTSPVDLPLILVGGGSMVIGDKIDGISEIIRPTYHEYANAIGAAVAKIASVVDTVISLEGGISFEKVLELKKQDAIEAIIKDKGAVRESTYIADVTTLPVQCSTNKVRIIIKAAGEVDLTKAFELHNRDSIRSEIKKVVKPEATFQTKVLDYKPDIKNGIWNISQTDLKWFSMGCYVLGCAGGGSPQCEYLEVRNILDEGYEIKIQDHEAIGDDDIIAWGGIMGSPAVSIERLGGTDTVDALAEVLKFTHNEDCQALMCLEIGGANGLQSLIMASSKYFNKPLLDCDWMGRAYPTYYQTTLAAHTTGLLAPAAISDGVGNSLILTSANSDVLTDVILRASCGEMGSRVGYAGRPSEGHLVKKFAVKNSYSLAWRIGRQIAYCQETNSTDQVADRIIEEVGGQSSAKKLFTGKITSVEQKLYKGHSYGQIVITAFDAEDQDISSSDIPFVETKGTLRVPYKNEIMYAEHEFDSKKKILASVPDLISVIDTQSGQAIGVPEFKYGLRVIVIGIAGSDLWSDCEKSRELGGLKAFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9I9</accession>
    <name>A0A1E3P9I9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_59961</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041091.1">
        <property type="nucleotide sequence ID" value="XM_019182908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9I9"/>
    <dbReference type="STRING" id="683960.A0A1E3P9I9"/>
    <dbReference type="GeneID" id="30200154"/>
    <dbReference type="OrthoDB" id="5865767at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37283">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN YHR131C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37283:SF1">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN YHR131C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="348"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="61199" checksum="F50060449BC2829A" modified="2017-01-18" version="1">MSSSSSSASSTTSLSLLSSARSKDSSSAVAPPHIRYQTSPLSNMSSIEYQQQHQQESNNNITNTNTAGDSSSQMDYLEYLQKQPCHPPTYSAANASRNIRYPIYEQVETHQLPDYSPSINQVALVLRKVEWLSPYEPSPIRSWKNLVMELNSTQLNFYSIDNLPLLHRVKKSKTKKYYEFTPIENDFILNAVKKKPGDYLTTKRLVKSYSLQFAKFGLPVDYKKKNNCLRVRAETEQFIIHFHSIDEMISWSNYLSVGINVSLDIDTRPMPSDRTVPRRRRNRRCSSSNRNRSYSDSELIRRELTPTKRRSSFSGESSIRNKISRIFNRKKSNPSSFDIEELSKSFQQSSLTSSTTPSSSSSIPTSQHERSASLPNSTSLESSSEPVSRSPTDEAESIRNEMTTIREEEEEGEANSVHELHDEEDDESDEDEDDLSIHHTNQSAADMLFNDYQFNFHEKIWKPEHKIMTRRKFLKDSLRCIKLLPGDEEWVGKCLVRSSNPPKFETSNQIKSKSSKNKFVKEYIVGPQGLVSVCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA05</accession>
    <name>A0A1E3PA05_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_60265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041405.1">
        <property type="nucleotide sequence ID" value="XM_019182964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA05"/>
    <dbReference type="GeneID" id="30200210"/>
    <dbReference type="OrthoDB" id="10418796at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="184" mass="21078" checksum="E8A535045E3715AB" modified="2017-01-18" version="1">MASIIKPHLLVFVNYVKPLQTAAEARYIYSQFAKLGTLSWWHDYASPAGYRHHGARSVAVYTLDNTRDSDLLELERKTLTDDLMSIVGLPTMHDWDLLESGKAKRIEPNYSFLDITPTPHSLTKRKMPEFIPKPHHVIVKSATKYEPFFIATTREQDVGYMSKYVNMKASDSIPIKIHLANTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA24</accession>
    <name>A0A1E3PA24_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Cysteine proteinase 1, mitochondrial</fullName>
            <ecNumber evidence="2 9">3.4.22.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_76397</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Has aminopeptidase activity, shortening substrate peptides sequentially by 1 amino acid. Has bleomycin hydrolase activity, which can protect the cell from the toxic effects of bleomycin. Has homocysteine-thiolactonase activity, protecting the cell against homocysteine toxicity.</text>
    </comment>
    <comment type="function">
        <text evidence="7">The normal physiological role of the enzyme is unknown, but it is not essential for the viability of yeast cells. Has aminopeptidase activity, shortening substrate peptides sequentially by 1 amino acid. Has bleomycin hydrolase activity, which can protect the cell from the toxic effects of bleomycin. Has homocysteine-thiolactonase activity, protecting the cell against homocysteine toxicity. Acts as a repressor in the GAL4 regulatory system, but this does not require either the peptidase or nucleic acid-binding activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.</text>
            <dbReference type="EC" id="3.4.22.40"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homohexamer. Binds to nucleic acids. Binds single-stranded DNA and RNA with higher affinity than double-stranded DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.40" evidence="2 9"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041425.1">
        <property type="nucleotide sequence ID" value="XM_019184377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA24"/>
    <dbReference type="STRING" id="683960.A0A1E3PA24"/>
    <dbReference type="GeneID" id="30201623"/>
    <dbReference type="OrthoDB" id="2666448at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070005">
        <property type="term" value="F:cysteine-type aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043418">
        <property type="term" value="P:homocysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00585">
        <property type="entry name" value="Peptidase_C1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000091">
        <property type="entry name" value="Aminopeptidase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004134">
        <property type="entry name" value="Peptidase_C1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363:SF2">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03051">
        <property type="entry name" value="Peptidase_C1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005700">
        <property type="entry name" value="PepC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0788">Thiol protease</keyword>
    <feature type="active site" evidence="10">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="361"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005700-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="448" mass="51681" checksum="E74ECAF131AFC606" modified="2017-01-18" version="1">MTLSVKDLQNWQQEFDQDVQYQLASSTLQQYNVDDLLISSKTKLPNVFTHVIEAEGAPVTNQKASGRCWLFASTNILRILAAKNLNLKEFQLSQNYLFFYDKLEKSNYFLDKILETAGEDVNSRLIQELLSAPVNDGGQFTMFLNLIEKYGVIPQDQYPDVYSSTASRKLNELITTKLREFAEILRNLVKDKASEAEIEKVKNDQVKEIYKYLTIFLGVPPQPTEQFTFEYYDKDKKYQKLTSTPLDFLKEYVKFDYSKPISLINDPRNPYNSKVKIEHLNNVSKGREVEYLNLDNDTLSSLIVKRIKANKPVFFGSHTPKYMNKKKGIMDVDLFDYKLIGFENGQSKASRVLYHESLMTHAMLITGVSVDDNGKPVRYRVENSWGKDSGIDGYYIMTQDYFEQYCYQIVVDIDEIEEHKALLDKEPIVLPLWDPMGALAVCGRETSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAD9</accession>
    <name>A0A1E3PAD9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_26630</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041119.1">
        <property type="nucleotide sequence ID" value="XM_019181283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAD9"/>
    <dbReference type="STRING" id="683960.A0A1E3PAD9"/>
    <dbReference type="GeneID" id="30198529"/>
    <dbReference type="OrthoDB" id="5580488at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043224">
        <property type="term" value="C:nuclear SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140299">
        <property type="term" value="F:molecular sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046685">
        <property type="term" value="P:response to arsenic-containing substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046686">
        <property type="term" value="P:response to cadmium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22147">
        <property type="entry name" value="F-box_SpPof1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000016">
        <property type="entry name" value="E3 ubiquitin ligase complex SCF subunit sconB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000715">
        <property type="entry name" value="F-box protein MET30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051075">
        <property type="entry name" value="SCF_subunit_WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872">
        <property type="entry name" value="UBIQUITIN LIGASE SPECIFICITY FACTOR/HREP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872:SF9">
        <property type="entry name" value="UBIQUITIN-BINDING SDF UBIQUITIN LIGASE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="145"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="270"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="310"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="350"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="389"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="492"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="533"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="452"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="593" mass="68150" checksum="1342731CD7657CD6" modified="2017-01-18" version="1">MDLRKSCNPNKFNYDKSSNITELKQDVINDDEAIITDEPNDGINSAENIVSNTNLIPSDNYHKYCYRHNPDVQCNKQSDSIKMNEIQKNLEKLPLKHQHFINSIWSSFSNSNTFQRNLILQGILTQCCFPQLSFISQEISSLIRIDFISTLPFEISLKILCYLDCASLCNAAQVSKKWQKLADDDRVWHHMCEQHIDRKCPNCGWGLPLMHMKRAREIKIEHEPKTKKRKLSSSSTGSPEVKTRPWKTVYSERFKVERNWRKGLFKSREFKGHKDGILTLKYDYKYLFTGSYDCSIKIWDINTGEVLKTLESHTKGVKSLVFDEQKLITGSFDHTIKVWNYHTGQCISTYRGHTDSVLSVDFFKKYIVSGSADKTLKIWNVETRTCYALRGHLEGVNSVKIHSKSLTVFSGSDDATIRMWDLKTNKCLKIFKGHIGQVQKVIPLNIPEDYDLVKEDEDDEKDEDDEDEEEEEDNEEFGEIDYSDETRQYPTYILSASLDNTIKLWNVKTGKCIRTQFGHVEGIWDISADTFRIVSGAHDRSTKIWDLQSGKCMNTFAGHNSPVTCVGLGDSSFASGDDTGVVRMVNFDVDTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAF8</accession>
    <name>A0A1E3PAF8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_60465</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPRTase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="4"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041613.1">
        <property type="nucleotide sequence ID" value="XM_019182997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAF8"/>
    <dbReference type="STRING" id="683960.A0A1E3PAF8"/>
    <dbReference type="GeneID" id="30200243"/>
    <dbReference type="OrthoDB" id="106623at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008655">
        <property type="term" value="P:pyrimidine-containing compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000023">
        <property type="entry name" value="Probable uracil phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001097">
        <property type="entry name" value="PRK00129.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="10">
        <location>
            <begin position="13"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="216" mass="24580" checksum="3481C96EAFD85DD6" modified="2017-01-18" version="1">MSTEEYKNVLLLPQTNQLLGLYSIIRSKETRRSDFVFYSDRIIRLLVEKGLDQLPVEKHTVETPLNVDYEGVKFLGKICGVSIVRAGESMEQGLRDCCRSVRIGKILIQRDEETALPKLFFEKLPEDISDRYVFLLDPMLATGGSAMLATEVLISRGVKPERIFFLNLLTSPEGIKNYHSKFPEIKIITGGIDEKLDDKKRLVPGLGDFGDRYYCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBQ9</accession>
    <name>A0A1E3PBQ9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_76997</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042042.1">
        <property type="nucleotide sequence ID" value="XM_019184484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBQ9"/>
    <dbReference type="STRING" id="683960.A0A1E3PBQ9"/>
    <dbReference type="GeneID" id="30201730"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000282">
        <property type="term" value="P:cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010513">
        <property type="term" value="P:positive regulation of phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000412">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0342">GTP-binding</keyword>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="24"/>
            <end position="31"/>
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        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="31"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
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        </ligand>
    </feature>
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        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
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        </ligand>
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        <location>
            <begin position="130"/>
            <end position="133"/>
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        <ligand>
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    </feature>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ62835.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="21436" checksum="ADB5B3D87CB78CAB" modified="2017-01-18" version="1">MGGSVSKIMGKLFGTKEMKILMLGLDSAGKTTILYKLKLNKIKTTAPTVGFNVETFQYKNVKFNMWDVGGQDRLRPLWRHYYPKTNALIYVIDSSDKTRLEESKRQLYKVLSELSGNATTKGFLLLVFANKQDLKNSMSPKEISEFLELKQNITKDQLYAVIGTNALTGQGLIEGLSWISNNFKPKTTPN</sequence>
</entry>
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    <accession>A0A1E3PBT7</accession>
    <name>A0A1E3PBT7_WICAA</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="3" type="ORF">WICANDRAFT_60884</name>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="235"/>
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        </location>
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            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
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            <end position="246"/>
        </location>
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            <end position="256"/>
        </location>
    </feature>
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        <location>
            <begin position="257"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="347" mass="39988" checksum="71FE0D693846E000" modified="2017-01-18" version="1">MEELQPDETNNNSTMIKGKFKKTVLRRDSNSAPFRPAPIEAKPSDSSIPTSELTSLSRPRTRDDESIFVNTGKVSPVIKEPPRFSRRSTFSEESPLRKTSDVDDDNTAIDEFIDRLNIGKIGDFLKKIIIAIWKRLQNSKYINSDNDGRIHLQSFCIGVILTSVVVMIQPFLVVYLDSWIVLLGRLFKHLMAWVMVAAVLSYAFNTSKKAKQDKESHEAVSPRLNNFEKLHYRVKNSSNNSSRSTSPTKTLKNKNGSRPSFSRQSFSSASIPRRDSTDSYNDQMILMNITERTQQDEKFRIDHHNSDKPKDNYEKFMDVAYKKNQEHDIYNKFVNDSKENVRRLSKH</sequence>
</entry>
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    <accession>A0A1E3PC11</accession>
    <name>A0A1E3PC11_WICAA</name>
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        <recommendedName>
            <fullName evidence="4">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_15016</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
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                <person name="Otillar R.P."/>
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                <person name="Peng Y."/>
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                <person name="Rosa C.A."/>
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                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
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                <person name="Grigoriev I.V."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="OrthoDB" id="636685at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008623">
        <property type="term" value="C:CHRAC"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="HFD_Dpb3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF151">
        <property type="entry name" value="DNA POLYMERASE EPSILON NONCATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="4">
        <location>
            <begin position="6"/>
            <end position="68"/>
        </location>
    </feature>
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        <location>
            <begin position="102"/>
            <end position="131"/>
        </location>
    </feature>
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        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="131" mass="14496" checksum="58E381FC1B5B5F7B" modified="2017-01-18" version="1" fragment="single">SGTSVLPLSKIKKIFKTDPEHVSASEAAVFSTAIATELFIQYFTEQASLIARSEKRKKLQYKDFASAVGNIEQLYFLSDTVPKTVNLKNLVEQNKVNYSAKAQNQQPQALAKGQQTLNLGKSTANKNDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC60</accession>
    <name>A0A1E3PC60_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="15">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_67145</name>
    </gene>
    <organism evidence="17 18">
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        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 15">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="15"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042165.1">
        <property type="nucleotide sequence ID" value="XM_019184072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC60"/>
    <dbReference type="STRING" id="683960.A0A1E3PC60"/>
    <dbReference type="GeneID" id="30201318"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000037">
        <property type="entry name" value="Calcium-transporting ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000014">
        <property type="entry name" value="Calcium-transporting ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000028">
        <property type="entry name" value="Calcium-transporting P-type ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000065">
        <property type="entry name" value="Sarcoplasmic/endoplasmic reticulum calcium ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005782">
        <property type="entry name" value="P-type_ATPase_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01116">
        <property type="entry name" value="ATPase-IIA1_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 15" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="11 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="288"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="748"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="821"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="919"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="955"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="16">
        <location>
            <begin position="3"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ62958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="993" mass="108758" checksum="E1DFC6D68F7D46F6" modified="2017-01-18" version="1">MTAPYNKTVELVLKELEADSEIGLSSLVAEERLSKYGPNSLPKESSTPLWKLILGQFEDQLTLILLASAVVSFGLAVSDGDLSWSSLIDPIVILSILVLNAIVGVQQESSAEAAISALNEYSSSDVKVLRNGKLVHVKQELLVPGDLIDLSVGDIVPADARIVKIFSQTLRVDQSILTGESESVIKDTEPIPIEDAVKQEQINVVFSGTTIVSGHARAVVILTGEKTAIGDIYTDISSQISQPTPLKEKLDDFGDLLARFITVICIAVWLININNFNDPAHGGYVKGAIYYFKIAVALAVAAIPEGLSVVITTCLALGTKKMAKQNAIVRSLSSVETLGSTNVICSDKTGTLTTNQMVVHNFVYFKNQTDLSTLTISGHSFEPEGTVNDEDGNSIELPDSRYPILHKVSQICSICNDANVVQIDQNTYKNVGEPTEAALKILVEKLSGSAQQDLAVTSVTPVSDLYNKEYPRLATYEFTRDRKSMSVLVQSGGNKSELLVKGAPEDIIARSTNYLNQTNNTLRVDRLSHEYRAELLTTVEHFAAEGYRIIALAYKEDVDLDLAHKATTSQDYVHLEQNLTLVGFAALIDPPRPEVIQAIKECKDAGIRVIVITGDSPITAENISKQIGIFKDDEDTSDLILTGREFISLSDDARAKAAEKVKLFARVEPSHKSLLVDYLQKSGKIVAMTGDGVNDAPALKKADIGVSMGSGTDVARLASDLVLQDDNFATIVTAVKEGRLIYNNTRQFIRYLISSNIGEVVSIFLTAALGLPEALIPVQLLWVNLVTDGLPASALGFNPPDLKIMSKPPKSKDEPLVSGWLLFRYIVVGTYVGVATVFGYAWYFILYEQGPQITFSQLSHFHQCSANFPEIGCEMFHDSHAVRGSTISLTILVIIEMFNAMNNLSESDSLLSFPLWKNIYLILAIILSIVLHFAILYVPWLAVLFNVEPLNKDEWYAVVIISLPVIVLDELFKLYERLFLIPKVRRSDSKKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC96</accession>
    <name>A0A1E3PC96_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">OPT family small oligopeptide transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_75772</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042250.1">
        <property type="nucleotide sequence ID" value="XM_019184258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC96"/>
    <dbReference type="GeneID" id="30201504"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00727">
        <property type="entry name" value="ISP4_OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="321"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="391"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="448"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="477"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="508"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="626"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="668"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="705"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ63043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="766" mass="86443" checksum="3F85492A2D47D071" modified="2017-01-18" version="1">MSIHDPEKNINETNFISKDEHILDSTINFEESSNDLKLNNYSSNSSGSQKLSEAVDITEDDVNAIVPTKDYEGDANTVRMWVLAFVFGTVIAGVDSFFQMRFPTITIGAIVAQVVSYPVGRLWYLVLPNWSLPLPMGRKISLNPGPFNQKEHACIYMFVNYVASAGLVNNSVVEQFRFFNMNIGIRRMILFQLSSYLFAFCLAGLARPILVTPANVVWPGLLSSCALFQTFHNPTNEPAGKWKISRFHFFAIVFVCSFVWFWFPDLILPFLSTIGAWISWIRPENATLSQVFGVNTGLGLFPLTFDWTQITSLSNPLSTPFWSIGCIFVSFVFWIWIVMPALYYQNKWQVAHFPIMTSSIYNVKGKSYDTSAVVDKQYRLDYDKFKQYSPVMLPIAFLMNVALGLAAFSSMMIAFFFRFKSDVIGPLRNKTTDVHNDALAKYESFPRWFYLIWGVIGLAFGFAFFEGFNHETQLRAGGYIVSMVISACVFLPLALIESRSNFEVSLAAFFEIIAAFWFSGQPIGLMYFYMMGFGTMQHAMHASQGAKVGHYMKVPPKTAMTLLLVGSIWAACVSPSVTGYILNHFKDVCTAEAKNHMVCRKSKTQFNTLVVWGLFGKHIFASGGRYVWVLWFFLLGALVAAFLSLLQWKFPKHIILKKINPTLFFGGAANIPSVTGFNYSTWFVVGFILNFIIHRRKHVWWKKYNLVLAVGLDCGVAIAALLIYFCVVYTGGSSNYNWWGTEVSKSGCDSKGCPHLTSKVIPPTGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCK3</accession>
    <name>A0A1E3PCK3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">60S acidic ribosomal protein P0</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_89374</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. uL10 forms part of the P stalk that participates in recruiting G proteins to the ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042227.1">
        <property type="nucleotide sequence ID" value="XM_019186181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCK3"/>
    <dbReference type="STRING" id="683960.A0A1E3PCK3"/>
    <dbReference type="GeneID" id="30203427"/>
    <dbReference type="OrthoDB" id="10259902at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05795">
        <property type="entry name" value="Ribosomal_P0_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1730:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030670">
        <property type="entry name" value="uL10_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039087">
        <property type="entry name" value="L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="7">
        <location>
            <begin position="109"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="285"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="285"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="295"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR039087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ63020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="313" mass="33595" checksum="275C9F9149A5093F" modified="2017-01-18" version="1">MGGVREKKVEYFAKLRELLEEYKSIFIVGVDNVSSQQMHEVRKALRGKAVILMGKNTMVRRAIRGFLSELPDYEKLLPFVKGNVGFVFTNVDLKEIRDIIVANKVAAPARAGAYAPADVWVPAINTGLEPGKTSFFQALGVPTKIARGTIEIVSDVKVVEAGAKVGQSEASLLNLLNISPFTYGLAVLQVYDNGQIFPPSVLDITDDELVGHFVSAINTIASISLAIGYPTLPSVGHSVVNSYKDLLAVAIATKYVYPEIEELVDRIENPDKYASAAPAAAAATSAASEAAPAEAAEEEAEESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PDI6</accession>
    <name>A0A1E3PDI6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_27416</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042134.1">
        <property type="nucleotide sequence ID" value="XM_019181370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PDI6"/>
    <dbReference type="STRING" id="683960.A0A1E3PDI6"/>
    <dbReference type="GeneID" id="30198616"/>
    <dbReference type="OrthoDB" id="73612at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013861">
        <property type="entry name" value="TMEM115/Pdh1/Rbl19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13377">
        <property type="entry name" value="PLACENTAL PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13377:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08551">
        <property type="entry name" value="DUF1751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01160">
        <property type="entry name" value="DUF1751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="401" mass="44764" checksum="F0CD8A914FABDEA7" modified="2017-01-18" version="1">MARSSVPPVTKLFLALLVSCSLITFVLRYSTYLHIVTSETHDQDHVHLIGEDQAASDNENSVPTPQELYVPLLALVPGQTSLIKMPWVLFTTSFIEDGVLSFALCFFLILYTGRYIEVLWGPREFFAYLTSNILISNVIAYSYYSFKLSVDDSLPISPITGSSALIISLLVAIKQRIPNHYLLFLNGFIRLRVSLVPFFVLSTGLIFSFITSNPYYHVINQLAWLGFIISWVYLRFFKEGGSGRQLSLLPITESGNPTILIKGDRTGQFALSTFFPRPLSFIVAFISDKIFQMFVTLKLLNHGDFTSNNEEDMQDEVSNSNTQTDLISQMSQSRLFTSSSLAGVSGNISTVSLPKTGFIKTVHDKLFALIGQSNDQSAQENRTLASRRKQALAQLDSKLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PDI9</accession>
    <name>A0A1E3PDI9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Monothiol glutaredoxin-5, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_77170</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042219.1">
        <property type="nucleotide sequence ID" value="XM_019184512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PDI9"/>
    <dbReference type="STRING" id="683960.A0A1E3PDI9"/>
    <dbReference type="GeneID" id="30201758"/>
    <dbReference type="OrthoDB" id="415696at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990229">
        <property type="term" value="C:iron-sulfur cluster assembly complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015036">
        <property type="term" value="F:disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044572">
        <property type="term" value="P:[4Fe-4S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000005">
        <property type="entry name" value="Glutaredoxin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00365">
        <property type="entry name" value="Grx4 family monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="GLUTAREDOXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293:SF16">
        <property type="entry name" value="GLUTAREDOXIN-RELATED PROTEIN 5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="7">
        <location>
            <begin position="46"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ63012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="144" mass="16090" checksum="F08DA2A872833859" modified="2017-01-18" version="1">MFKTSFLRPISHFRSPVLRSTQSSFIRLLSTETKDAIEKAVASAPVVLFMKGTPEFPQCGFSRATINILGTTGVDPEKFAAYNVLEDQELRDGIKEFSEWPTIPQLYVEKEFVGGCDIVTSMAQSGELTELLEKKNLLVPLEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUH1</accession>
    <name>A0A1E3NUH1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_36893</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035983.1">
        <property type="nucleotide sequence ID" value="XM_019182484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUH1"/>
    <dbReference type="STRING" id="683960.A0A1E3NUH1"/>
    <dbReference type="GeneID" id="30199730"/>
    <dbReference type="OrthoDB" id="5841748at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08022">
        <property type="entry name" value="M28_PSMA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02121">
        <property type="entry name" value="PA_GCPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000008">
        <property type="entry name" value="Glutamate carboxypeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000101">
        <property type="entry name" value="N-acetylated alpha-linked acidic dipeptidase like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF46">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="4">
        <location>
            <begin position="222"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="6">
        <location>
            <begin position="399"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="5">
        <location>
            <begin position="663"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="11"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="284"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ56776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="789" mass="89348" checksum="4BEF84DA20A1B555" modified="2017-01-18" version="1">MSEERLPLLLGTSSNTNSNSNQRPTRRGDYSRARSASAVSAISTSIESVKEFIKDNWPSLTTQKFVAGILWAIVAVVIFNLIFLPRTSLGRDYRRIHFSTLSKVESQRLFLKSLSDENNASDLLYNYTHSPHLAGEGDDLTQFTIDKFKEYGFKPYKEKYQIWYNKHIANGVSLINSDDEVVYTPTLLEDELKQDKASNNTLSTPAFHAYSANGNVTSRYIYANYGTKEDYAKLELAGVQTNGTIHIIRYTKLFRGIKVKNAERAGAVGVLMYTDALDDGEITEKNGYKSYPEGPARNPSSIQRGSVGYFTDQPGDPTTPGFASSRLGKRQDPSPFMPGIPSIPISAKEVGPLLEKLNGRGSSLGWSGDVDGFDYSTGPSDYKINMFNEQQYSIKPITNILYVIPGLLNEKIFIGNHRDSWTVSGAGDPNSGSAILLEITRAFGHLVKKGWKPLRTIVVASWDGEEFGMLGSTEYGEYHLRDLKNNVIAYLNLDMGVIGHKLQVDSNPLLHHFLIDTSKYIPFNGQDNYTLYDYWKDQDDNHIDIIGAGSDFTVFQHHLGIPSCMVYFNNDGKDAPLHVHSNYDSYHWMSTFIDPDFKLHNTLAKYFGFLALSISENEVFEYRVAHYAKEISKYFQSLKEQAPHGWLEKHLEKSKTVGKAIEDVEFALSEFYSYADMFDMRAKLLQKQISIDYPWFKIYKKIKLAIDIKLLGNRAKKIDRLFVDDKGLKNRPWMKHVLFAPDRENGYNGDILPGLHEAIIDNDLEEFKEWLGIILKKVKSATTKVMINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUU5</accession>
    <name>A0A1E3NUU5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Glycoside hydrolase family 17 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_36806</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Glucanases possibly play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036070.1">
        <property type="nucleotide sequence ID" value="XM_019182477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUU5"/>
    <dbReference type="GeneID" id="30199723"/>
    <dbReference type="OrthoDB" id="941679at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000111">
        <property type="entry name" value="Soluble cell wall protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF14">
        <property type="entry name" value="FAMILY 17 GLUCOSIDASE SCW10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ56863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="260" mass="28584" checksum="466EF99675C47EF3" modified="2017-01-18" version="1" fragment="single">AAGAKGITYSFYTSAGSCKTPEDIQRDISMIKNFEVIRIYDTDCDGLANILKSMGPNQKIFAGIHYPEVVDASVEIIGRAIQEQADGDWSKIDTVSVGNEMVNFGKATPDQLQASINRARELLRGKGYNGPVVTTDTLVAVLNNRDLCHVSDYIAVNSHPYWDGGVPAQQAGPWLQSQLQMLDDVCGRNGKQIFLAETGWPHKGKKFGQHGDPSKDNQLVAVKSIVDTLGPRTILFTTFNDYWKDGGTHDVEKFWGIFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV76</accession>
    <name>A0A1E3NV76_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable 26S proteasome regulatory subunit p27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_58552</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036210.1">
        <property type="nucleotide sequence ID" value="XM_019182826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV76"/>
    <dbReference type="STRING" id="683960.A0A1E3NV76"/>
    <dbReference type="GeneID" id="30200072"/>
    <dbReference type="OrthoDB" id="72325at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000107">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040815">
        <property type="entry name" value="Nas2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035269">
        <property type="entry name" value="PSMD9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12651">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12651:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18265">
        <property type="entry name" value="Nas2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="3">
        <location>
            <begin position="83"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00068021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="203" mass="22820" checksum="845CFDE2000108A7" modified="2017-01-18" version="1">MNSLKDVEYNLSHDINTIKDLNYQQLALVKSEIESELNKLFEVLQKNKVSMDSALITHDGFPRSDVNVIEIRNVRVKIIKLRNDLTIVINALSEKLHSQFQNTEPEKKIDSDLLIPFAKVTEVVDSGPAAQAGIRVDDKIVIFGTINVTNHNNLQNIGLIVKNNVNKPIEIKVLRGSQKLHLEIKPTSDWPGNGLLGCRIIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVF6</accession>
    <name>A0A1E3NVF6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_81317</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036300.1">
        <property type="nucleotide sequence ID" value="XM_019185213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVF6"/>
    <dbReference type="GeneID" id="30202459"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="279" mass="33031" checksum="FF6F3B184C70619B" modified="2017-01-18" version="1">MLGIRSYYRVQDSTPQTIKEDPELDEDLEDIEICDENEFNNVMILQDSKSQEKLRMLHRLIVKRDGLKIRLPQHWEMDLIFTEILTKENFGHYGDAEYEIMLPFEIFMIENIHYKGLVKFKNILNLPNKIRGMSLTNKNGLKKTTQLISTTIASYVTWLIDLSFNTHEEKRAYMKLFKSEYLVVTKSETCSKSRNGTMNAISDKILSQSENHLIYHIDIINSTVTFSVIDEKRLFKLIKFNLKRDFQRVQKFQNQIDIEFALKLTMLIEMARKEMIEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVF7</accession>
    <name>A0A1E3NVF7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_24001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036292.1">
        <property type="nucleotide sequence ID" value="XM_019181010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVF7"/>
    <dbReference type="STRING" id="683960.A0A1E3NVF7"/>
    <dbReference type="GeneID" id="30198256"/>
    <dbReference type="OrthoDB" id="347657at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000196">
        <property type="term" value="P:cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05581">
        <property type="entry name" value="STKc_PDK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000128">
        <property type="entry name" value="Serine/threonine-protein kinase ksg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000534">
        <property type="entry name" value="Serine/threonine-protein kinase PKH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039046">
        <property type="entry name" value="PDPK1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF163">
        <property type="entry name" value="3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="36"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ57085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="299" mass="34357" checksum="985DC8FE60BD84AD" modified="2017-01-18" version="1" fragment="single">SEDWDKKGAAQMVKRETDELTGETEEIIIRRSVKDFKFGKTLGVGSYSTVLLATDKNTGRNFAVKVLDKRHIIREKKVKYVNIEKNTLNRLGKRDGIIHLFFTFQDESSLYFVLDYASNGELLNLIKQYGSLNEETTRYYGAQILDAIKYMHDNGVVHRDLKPENILLDDRKRVQITDFGTAKLLEKNDAGRYPSDTKAKSFVGTAEYVSPELLNEKAIGKPCDIWAFGCILYQMIAGKPPFKATNEYLTFQKIVKLQFAFTAGFPMSCRDLIKKILVLNPKDRYTIKEIQQHHFFQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVL4</accession>
    <name>A0A1E3NVL4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc/iron permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_65614</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035939.1">
        <property type="nucleotide sequence ID" value="XM_019183950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVL4"/>
    <dbReference type="STRING" id="683960.A0A1E3NVL4"/>
    <dbReference type="GeneID" id="30201196"/>
    <dbReference type="OrthoDB" id="262547at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF210">
        <property type="entry name" value="ZINC-REGULATED TRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="114"/>
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        </location>
    </feature>
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        <location>
            <begin position="403"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="432"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="466"/>
            <end position="489"/>
        </location>
    </feature>
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        <location>
            <begin position="501"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="146"/>
            <end position="213"/>
        </location>
    </feature>
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        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ56732.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="523" mass="57880" checksum="E194670E58FF19CA" modified="2017-01-18" version="1">MISTFDDFIHNLFTNHQGWFLTALSSLMCIFGASIIYSDLLVKWVNPNSSFDIKSNERFLICGFSLSSGSLLFTSFFKLLPKGLEYFNKAASGDNDDDGNSDSHGDLLKPSLSLLVYFLIGIIACELLNYGIHYFTSESIVHCNHGGDSDEEHDHSHSHGSHSHSHSSSSEELENKLDHSHSHNHHTHNHSHQYDEESLLSNDEPHYGSSGETNPLVVQQTTVIQPNHDHSQDQDATGPVIATRKSFFDIALNKITHRGSAQCIGIDDEDCEGDPNCINEELTTNVANDLHNLNFYRGLAISKSKNSLVAYTTSSPHHNVIDGALEHGDLVPIHPSNIRLTNEPNLDLHQQHKDDHHHQINTPISKLLSIGLQTCLALTLHKFPEGFITYATSAADPKLGVSIFLSLAIHNIVEGFSMTLPLYLAFNSRLKAFGMTFILGGLSQPIGALLAYYLLAGREIDQKESFYIFGILMCITSGFLSVVSLQLFASSISFGGSANRVIRWCIVGIFIIQLSNVFTDYNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVU6</accession>
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="2" type="ORF">WICANDRAFT_81452</name>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ57233.1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVU6"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="172" mass="20018" checksum="36FC81F2511A7DA3" modified="2017-01-18" version="1">MCMAKPRHIYNKEYRATIDILMLPHDVMVDNLKIGDIQQMVFDKEELEKAKYNNISCPTASYNPYSDYSDSDSESELELEPSYGTAGFQKNEKSDLLPLPVTMKKPTKEKMTMVSKILLVLIGLMLMSLIIHFTSDYTMLKMNEMEVNESSVEEVEYKYTEKLDQLYKDFHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVW7</accession>
    <name>A0A1E3NVW7_WICAA</name>
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        <recommendedName>
            <fullName evidence="11 12">Complex III subunit 9</fullName>
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    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_18002</name>
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    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The complex plays an important role in the uptake of multiple carbon sources present in different host niches.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein, 2 core protein subunits, and additional low-molecular weight protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the UQCR10/QCR9 family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ57276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="Cytochrome b-c1 complex subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.260">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="QCR9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036656">
        <property type="entry name" value="QCR9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12980:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12980">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX, SUBUNIT X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05365">
        <property type="entry name" value="UCR_UQCRX_QCR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81514">
        <property type="entry name" value="Subunit X (non-heme 7 kDa protein) of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ57276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="63" mass="7233" checksum="46A8E29BDBD07493" modified="2017-01-18" version="1" fragment="single">YTSIYNTLIKRNSVYVGTIFVGAFVFQGFFDVAVTKFYENHNKGKLWKDIKGKYLEGGDDEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW07</accession>
    <name>A0A1E3NW07_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_35945</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036523.1">
        <property type="nucleotide sequence ID" value="XM_019182377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW07"/>
    <dbReference type="STRING" id="683960.A0A1E3NW07"/>
    <dbReference type="GeneID" id="30199623"/>
    <dbReference type="OrthoDB" id="4142200at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF7">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="306"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="373"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="412"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="440"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="23"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="468" mass="51508" checksum="39CCBBA2C1798EA7" modified="2017-01-18" version="1" fragment="single">MDQNVVNDEKKHRGHRTYKFPVILIIISLGGLMLGVETSSMPVFVGSAYFRKYFNNPGAVAQGIIAASNPAGAFFGCWASGFISDRFGRIAALQTGSTIWIGGSIVSVLAQNIPMVITGRAVKGFSVGIFSASLPVYVSEIFPQNKKGLGTSVMQWSLTWGIMLMFYISYFCNLLGDERAFRIAWGIEIAPAISLLVGTIFLPESPKWLATGSRWEEADHILEKLGIQPENRMGNQVGLIDMYDSTGSKCTYLDLFRKDLRKHLLVGITTQLFTQLSGIGVLMYYLVYICQMVGLSGEVKILSASIQYVINVLFTIFPILWLDKMRRKDVLVFGATFLGTCISTIGAIMGWYGHGVPPIDGNESIVWEVTGTPGSLCLALCFLFVAVFASTLSCAGWLYTNEILPLRAKAKGSAICMSVSWTVNFTLTFLTPLSMKHIKWFTFLIFGLFCISGAFIMGFLFPETYGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW56</accession>
    <name>A0A1E3NW56_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TECPR1-like DysF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_18381</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036437.1">
        <property type="nucleotide sequence ID" value="XM_019180784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW56"/>
    <dbReference type="STRING" id="683960.A0A1E3NW56"/>
    <dbReference type="GeneID" id="30198030"/>
    <dbReference type="OrthoDB" id="74314at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052816">
        <property type="entry name" value="Peroxisomal_Membrane_PEX28-32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010482">
        <property type="entry name" value="TECPR1-like_DysF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28304:SF1">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28304">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06398">
        <property type="entry name" value="Pex24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="TECPR1-like DysF" evidence="8">
        <location>
            <begin position="68"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="443" mass="52919" checksum="ED634F9F1C7AF5AF" modified="2017-01-18" version="1" fragment="single">DSDFEDEGTEVGEERTSTDIKREKSMAKQQNHFIDILLEKLLVHAVIPDKQDLETRLNDPIRKETESLSIPKFTKNLRKMTAKLGGMFQAQYFILRIFHWTRPSLTISALMIYTFIVLHPTLLFILPLIFLLYGIMVPGYIYRHPIHKPKLLKVKQRGESLLEKFSKIENNKKMAKLLEEEKAFEEEEIERRVPELNEKIDTNVRLLKKNMEFLVNMRDLQNSLSNLLKMVSNVERFWYGTAGFKDERVSTSLFFTIFLTVCFFILIGPYIPWRLLLITMGWNLLLLVHPKVFPKIQEFNNLIKPQKKEIDKIVKDSERKDIILDEPPEIREVEIFEIFKKSVSSSQWEFYLFSKSIFDMTDQYRRAQKPPPGVATIDQVNPPKGWRFEDDDPWNIDYECKEWCQNRGLRLNRDNYIDMHNEFYIDDEFKRRRLFRNVIRYSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW64</accession>
    <name>A0A1E3NW64_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acyl-coenzyme A diphosphatase SCS3</fullName>
            <ecNumber evidence="8">3.6.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">FIT family protein SCS3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SCS3</name>
        <name evidence="8" type="synonym">FIT2B</name>
        <name evidence="10" type="ORF">WICANDRAFT_65699</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Fatty acyl-coenzyme A (CoA) diphosphatase that hydrolyzes fatty acyl-CoA to yield acyl-4'-phosphopantetheine and adenosine 3',5'-bisphosphate. Preferentially hydrolyzes unsaturated long-chain acyl-CoA substrates in the endoplasmic reticulum (ER) lumen. This catalytic activity is required for maintaining ER structure and for lipid droplets (LDs) biogenesis, which are lipid storage organelles involved in maintaining lipid and energy homeostasis. May directly bind to diacylglycerol (DAGs) and triacylglycerol, which is also important for LD biogenesis. May support directional budding of nacent LDs from the ER into the cytosol by reducing DAG levels at sites of LD formation. May play a role in the regulation of cell morphology and cytoskeletal organization. Involved in phospholipid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + H2O = S-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:65568"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:156554"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(9Z)-octadecenoyl-CoA + H2O = S-(9Z-octadecenoyl)-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:65564"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:156553"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>an acyl-CoA + H2O = an acyl-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:50044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:132023"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>hexadecanoyl-CoA + H2O = S-hexadecanoyl-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:50032"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:132018"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Endoplasmic reticulum membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the FIT family. Fungal FIT2B/SCS3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="8"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036024.1">
        <property type="nucleotide sequence ID" value="XM_019183970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW64"/>
    <dbReference type="STRING" id="683960.A0A1E3NW64"/>
    <dbReference type="GeneID" id="30201216"/>
    <dbReference type="OrthoDB" id="5579088at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140042">
        <property type="term" value="P:lipid droplet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03231">
        <property type="entry name" value="SCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019388">
        <property type="entry name" value="FIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046400">
        <property type="entry name" value="SCS3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23129">
        <property type="entry name" value="ACYL-COENZYME A DIPHOSPHATASE FITM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23129:SF0">
        <property type="entry name" value="ACYL-COENZYME A DIPHOSPHATASE FITM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10261">
        <property type="entry name" value="FIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ56817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="253" mass="29099" checksum="5E0E50355957B99C" modified="2017-01-18" version="1">MAIQSNALSRLSHSLRLSNQELIAISIYPITILLGQIIYHSSPEIVNNYYTNKHNLLNVLFVKKGWFWTCLVDVYFVLQHGKSIQRAFIRLSIITVWWWCFTQSFFGLPLMDRVFIWTGGVCDYDIGLHGALDGIGSVVCRKVKGHWKGGHDPSGHVFLLVVGSLVLWFEILNTNILQELQSFITGFRNQLTIQNRLKYVLNHSIVFTLALLWLWWWMLLTTSIHFHSFIEQMSGLIAGCLGVLVYTIPRMVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWC9</accession>
    <name>A0A1E3NWC9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_35738</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribonucleoside diphosphate reductase small chain family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036632.1">
        <property type="nucleotide sequence ID" value="XM_019182347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWC9"/>
    <dbReference type="STRING" id="683960.A0A1E3NWC9"/>
    <dbReference type="GeneID" id="30199593"/>
    <dbReference type="OrthoDB" id="10248373at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005971">
        <property type="term" value="C:ribonucleoside-diphosphate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01049">
        <property type="entry name" value="RNRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033909">
        <property type="entry name" value="RNR_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030475">
        <property type="entry name" value="RNR_small_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409:SF18">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SUBUNIT M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00268">
        <property type="entry name" value="Ribonuc_red_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00368">
        <property type="entry name" value="RIBORED_SMALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="411" mass="46833" checksum="51A722D2CD32781C" modified="2017-01-18" version="1">MSTQETPSKATAKVLSGLDLDDSKKDLRAELSAAAARKEALATCNKPTESTVEETSKPKRTEESHKEFLAKHKSHRHQLKEAEKDEPLLVENKRRFVLFPIKYHEIWQAYKKAEASFWTAEEIDLSKDLHDWNERLNDNERFFISRVLAFFAASDGIVNENLVENFSAEVQIPEAKCFYGFQIMMENIHSETYSLLIDTYIKDPKQAEFLFNAIETIPCIKEKADWALRWIADDEALFGERLVAFAAVEGIFFSGSFASIFWLKKRGLMPGLTFSNELICRDEGLHTDFACLLFAHLKHKPSPEIVEKIITEAVAIESEYFTEALPASLLGMNSKLMCQYVEFCADRLLIALGNKKFYNVSNPFDFMENISLAGKTNFFEKRVSDYQKAGVMASTTKAETSSDAFAFDEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX48</accession>
    <name>A0A1E3NX48_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_97667</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036364.1">
        <property type="nucleotide sequence ID" value="XM_019186604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX48"/>
    <dbReference type="STRING" id="683960.A0A1E3NX48"/>
    <dbReference type="GeneID" id="30203850"/>
    <dbReference type="OrthoDB" id="497380at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101031">
        <property type="term" value="C:protein folding chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048027">
        <property type="term" value="F:mRNA 5'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000900">
        <property type="term" value="F:mRNA regulatory element binding translation repressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012959">
        <property type="entry name" value="CPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040059">
        <property type="entry name" value="PUM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13389">
        <property type="entry name" value="PUMILIO HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13389:SF0">
        <property type="entry name" value="PUMILIO HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08144">
        <property type="entry name" value="CPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PUM-HD" evidence="5">
        <location>
            <begin position="127"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="193"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="229"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="36"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="77"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ57157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="650" mass="73675" checksum="DB1C7CAEF1AC94E7" modified="2017-01-18" version="1">MGVAGRGTKRSGLPSKAAIKKSKISNTKAAKVQSESESEDEVSESEDELDVSSEDELDKDESEDELDQDEIKLGSASEDEGDEGEEEADGDSPKDGSKQTSKEQHAEQRKVLQERKLQRKSGVQVQHIKSLWERLRVKNPPVPKQVREKLCDEIWKLSKDVIRDLVLKHDASRVVQTLVKYSSKERREAVTTALKSHFYELATSSYGKYLLVKLLHYGTKDSRGLILDELHGKLRKLMRHREGAYVVEDLYTLYSSAEQKKQMIREFWGAEYAVFKESGKGKTIQEITSESVEKRTLIAKNLIGTITASVEKGSTGFQILHAAMKDYIQIINDEETRELIELLHEQFAELIHTPEGADVACFLLARATAKERKQIIKSLKEHAMSLIKNEYGNLVVITLFLTVDDTVLVSKALFGEYAEHLHELVVDKYSRRPFLYLLNGLDGRFFSPSVQKELKHYVELSQETSKKPNDQKKKELLDKISPAFYKSITDHTTEILSENIGSQFIGEVFLNNAPISDDKREEAISALIETFKGDFEEEEHPINKAFSVRLLKSLTQGGKWDAKTKTLIKLDVPGIGSDFATRFYEEVIGDSLEKWIENKDTSFIVVSIFESIDDKNAQFFKDLKKIKKDVKKASQEDNKGAQLLVKLAGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX53</accession>
    <name>A0A1E3NX53_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA 3'-phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_16833</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036757.1">
        <property type="nucleotide sequence ID" value="XM_019180712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX53"/>
    <dbReference type="STRING" id="683960.A0A1E3NX53"/>
    <dbReference type="GeneID" id="30197958"/>
    <dbReference type="OrthoDB" id="19045at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046404">
        <property type="term" value="F:ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046403">
        <property type="term" value="F:polynucleotide 3'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013954">
        <property type="entry name" value="PNK3P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006551">
        <property type="entry name" value="Polynucleotide_phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01664">
        <property type="entry name" value="DNA-3'-Pase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12083">
        <property type="entry name" value="BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE/KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12083:SF9">
        <property type="entry name" value="BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08645">
        <property type="entry name" value="PNK3P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="19448" checksum="D074BE23085E99EF" modified="2017-01-18" version="1" fragment="single">IISFDLDGTLVVTKTKGKFAKFPQDWKWFNYKTLTNLKQIKIPIVIFTNQGGVVATKTSKSYNNFHKRIELILEELGKRGVDVQNVWVYASPKKSASYKGDNEAQFDNMRKPNIGMFEEFLKDFGKDKINVEESLFIGDAAGRKNDFSKSDLRFAHGCQLKFITPEEYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX87</accession>
    <name>A0A1E3NX87_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Carbonic anhydrase</fullName>
            <ecNumber evidence="5">4.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Carbonate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_85887</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="5"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037016.1">
        <property type="nucleotide sequence ID" value="XM_019185965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX87"/>
    <dbReference type="STRING" id="683960.A0A1E3NX87"/>
    <dbReference type="GeneID" id="30203211"/>
    <dbReference type="OrthoDB" id="10248475at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03379">
        <property type="entry name" value="beta_CA_cladeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43175:SF3">
        <property type="entry name" value="CARBON DISULFIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43175">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="beta-carbonic anhydrase, cab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
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        </ligand>
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        <location>
            <position position="38"/>
        </location>
        <ligand>
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        </ligand>
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            <position position="88"/>
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        <ligand>
            <name>Zn(2+)</name>
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        </ligand>
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            <position position="91"/>
        </location>
        <ligand>
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        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601765-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003956"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ57809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="162" mass="18188" checksum="95C838B7D2862EA8" modified="2017-01-18" version="1">MSNASQFEINNKEFTKSFTQGSLPLPPSKKVAVLTCMDARLDPAKVLGIEIGEAHVIRNAGGRSIDGLRSLIISQRLLGTREIVLIHHTDCGMLTFKDDDLRNQLVKETGELVDHWSFLPFDDLKKSVIDDLEFLKKHPLILDVPITGYVYHVEDGSIEKVY</sequence>
</entry>
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        <lineage>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
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            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37451">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37451:SF1">
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="46"/>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF01284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
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    <sequence length="249" mass="27089" checksum="3567232CFFFDDAD2" modified="2017-01-18" version="1">MSEIQDIEKQQAKPFKSFRIISSTLFGFILRAAQWFVTILVLGLSAGSLDKYATSGSMSYTVFASVFTLLYLIVFTILSFVSPLNIIIGVNVILEILITIFWFTSFISIAAIYGPSDCNYHYSGWYYIYYINKNACQASKAAIAFAAVNFALFLVSALALILTSISNISTDQLFQQNSISGASLNRPLIAVTSIGTSTVAFQTDNTNIESADTLNNNTVEQEAEPKEEGAADDVSAQSPVTTVTPAQNN</sequence>
</entry>
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            <fullName evidence="6">Protein YIP</fullName>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="6">Multi-pass membrane protein</topology>
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        <subcellularLocation>
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    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR039765">
        <property type="entry name" value="Yip5/YIPF1/YIPF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822:SF2">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="7">
        <location>
            <begin position="41"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="231" mass="25953" checksum="93A27B25E203DD98" modified="2017-01-18" version="1">MVPNNFEGGLFSINHYRRYFNINTTDFFINVIRSINVVQKFEDQEEEIGDLYGPIWVTATVCFILFFSNTSSNLLKSWLIGGDDKYQYNFSLLTGAISLLYGYTVLIPFIFYMISVFYFKLTKFFTLSKIISIYGYANSVWIPSAIFGVLRGLLVNHSVLNGILKWVIVLIGGIVSAVSIGLKVYPVLKNSTIIVGNEKLSFGLMGCLAIAHLGFIIAIKVLFFGDLKVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXR2</accession>
    <name>A0A1E3NXR2_WICAA</name>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036679.1">
        <property type="nucleotide sequence ID" value="XM_019185128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXR2"/>
    <dbReference type="GeneID" id="30202374"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="261" mass="29399" checksum="9B370224A9AC2152" modified="2017-01-18" version="1">MKLSSFIFQTLTVHFISKIEAAPIPTLPSTITSRGNDNITSTGLLASSNDSVLSNSNIIEKHMGLSNINFNLNLPESIKSKVVIESTNTDHTQKIKNISILYKRSFPNPSNLSNFTIEERSNTKSCSIKFFSDPLVIFFLVSIYSYAAFMFGWLLTPFVSKFSDDLIALWYELDLSRNYSFREKFKITILHKGFYKFLGAATLAYTPGIHVFLILGAYYGVCFIIKLVQGKAIKKAREEANPPDIALDSFRRLPDGSFVRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY26</accession>
    <name>A0A1E3NY26_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD synthase</fullName>
            <ecNumber evidence="2">2.7.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">FAD pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">FMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_34404</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>FMN + ATP + H(+) = FAD + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.2" evidence="2"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037308.1">
        <property type="nucleotide sequence ID" value="XM_019182181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY26"/>
    <dbReference type="STRING" id="683960.A0A1E3NY26"/>
    <dbReference type="GeneID" id="30199427"/>
    <dbReference type="OrthoDB" id="270728at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23948">
        <property type="entry name" value="FAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000187">
        <property type="entry name" value="Probable FAD synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293:SF9">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293">
        <property type="entry name" value="FAD SYNTHETASE-RELATED FMN ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="13">
        <location>
            <begin position="57"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ58101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="247" mass="28838" checksum="E5709872FCB88E7B" modified="2017-01-18" version="1">MKFSEVCHESNLIVSRFLQINNDENDDGQLLIETQAKVQASLEVLDEALDKFNFEQLSISYNGGKDCLVMLILYLSSLSTKYSPDQLDSFNSLKSVYINYEQQFPELIKFINYSKSKYKLDLVSINMKLKQGFTQYLQYHPEIKAIIVGTRRSDPFASDLKHFQKTDHGWPEFIRIHPVINWHYDEIWCFIQKTQIEYCELYDKGFTSLGGIDSTIRNPSLKLQNGDYLPAYELKGDERERDGRNVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY70</accession>
    <name>A0A1E3NY70_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosome subunit biogenesis protein NIP7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_95304</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with pre-ribosome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NIP7 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037252.1">
        <property type="nucleotide sequence ID" value="XM_019186490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY70"/>
    <dbReference type="STRING" id="683960.A0A1E3NY70"/>
    <dbReference type="GeneID" id="30203736"/>
    <dbReference type="OrthoDB" id="27490at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd21146">
        <property type="entry name" value="Nip7_N_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21151">
        <property type="entry name" value="PUA_Nip7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.10:FF:000002">
        <property type="entry name" value="60S ribosome subunit biogenesis protein NIP7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.220:FF:000001">
        <property type="entry name" value="60S ribosome subunit biogenesis protein NIP7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040598">
        <property type="entry name" value="NIP7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055359">
        <property type="entry name" value="Nip7_N_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016686">
        <property type="entry name" value="Ribosomal_synth_fac_NIP7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005155">
        <property type="entry name" value="UPF0113_PUA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23415">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17833">
        <property type="entry name" value="pre-PUA_NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03657">
        <property type="entry name" value="UPF0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017190">
        <property type="entry name" value="Rbsml_synth_fac_NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PUA" evidence="8">
        <location>
            <begin position="95"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR017190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="181" mass="20563" checksum="9F1347C73FA711DC" modified="2017-01-18" version="1">MRPLTEEETKVVFEKLANYIGRNIAYLVDNKEDPHVFRLQKDRVYYVSDKIAKFATSVARPNLMSVGICMGKFTKTGKFKLHVTSLSILSKYAKYKIWIKQNGEMPFLYGNHVLKAHIGRMSDDIPEHAGVIVYSMSDVPLGFGVSAKSTSETRNQQPTAISAFRQSDIGEYLREEDTLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY72</accession>
    <name>A0A1E3NY72_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_107033</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN7/EIF3M family. CSN7 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036734.1">
        <property type="nucleotide sequence ID" value="XM_019180491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY72"/>
    <dbReference type="STRING" id="683960.A0A1E3NY72"/>
    <dbReference type="GeneID" id="30197737"/>
    <dbReference type="OrthoDB" id="10267031at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002183">
        <property type="term" value="P:cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045237">
        <property type="entry name" value="COPS7/eIF3m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7/DENDRITIC CELL PROTEIN GA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350:SF2">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="2">
        <location>
            <begin position="197"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="415" mass="47016" checksum="B8BD8E262B4C4AD0" modified="2017-01-18" version="1">MPGQSYITVDGSLADSITEYASILDLYNENTNYVEKLAAYAITPEGDDQDDDEDVEIEFKDQVFQEIADSYSHLAAIPDRDFESVGNLVVFIFTFSNEFELYLKQFLTNLIQVLGLQDVDVKVNKNKKKTLKITAIISVLSNVFNLIELENFEFRNFNLKLILNLVEILDNGSILLPIVSNVEGWLLSDIDSKPLEVQEETRLLILQLAKLISSSNELESLKLFELAITKIPNVSSNLINQYLIENLNSLKALDLSKILELEVVIKNHQESQEYLKLLQNYLSLTTLEFESSLSQFTSLTSINFKTLLKKNQFLTLSKIALTTNSITYTTIASSLNLSSNDVELFIFDAIKSGVVEGKLSQINQTFQVYKVNLIVKSIELKDWLEIKGKLLEWRSKLKDVRALVDQASKKKAVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY90</accession>
    <name>A0A1E3NY90_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein mS37</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_33845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the mitochondrial small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the mitochondrion-specific ribosomal protein mS37 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037343.1">
        <property type="nucleotide sequence ID" value="XM_019182116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY90"/>
    <dbReference type="STRING" id="683960.A0A1E3NY90"/>
    <dbReference type="GeneID" id="30199362"/>
    <dbReference type="OrthoDB" id="2210at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017264">
        <property type="entry name" value="Ribosomal_mS37_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28066">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN MRP10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28066:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037706">
        <property type="entry name" value="MRP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR037706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="90" mass="9985" checksum="316BFE308E359B98" modified="2017-01-18" version="1">MANKPVRLPPLPRLRVKKPVSQTQANPCVVVMSTLLNCWSSNGEGAAECKQLESSLKECMEINKKPVVVKSSINYHASRLYPKINGKPHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY99</accession>
    <name>A0A1E3NY99_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_34174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037282.1">
        <property type="nucleotide sequence ID" value="XM_019182159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY99"/>
    <dbReference type="STRING" id="683960.A0A1E3NY99"/>
    <dbReference type="GeneID" id="30199405"/>
    <dbReference type="OrthoDB" id="75169at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.10">
        <property type="entry name" value="Diaminopimelate Epimerase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003719">
        <property type="entry name" value="Phenazine_PhzF-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00654">
        <property type="entry name" value="PhzF_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13774:SF32">
        <property type="entry name" value="ANTISENSE-ENHANCING SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13774">
        <property type="entry name" value="PHENAZINE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02567">
        <property type="entry name" value="PhzC-PhzF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016184">
        <property type="entry name" value="PhzC_PhzF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54506">
        <property type="entry name" value="Diaminopimelate epimerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="1">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR016184-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="279" mass="30106" checksum="99F45FA4B8B1E4C1" modified="2017-01-18" version="1">MGQFKQVDVFTSTKFQGNPVAVFFEADNLSTQEMAKIANWTNLSETTFVLKPSSNKADYKLRIFTPGSELPFAGHPTIGSCHALLEAGLITPQTGKIIQECGAGLVELTVTDDDISFKLPYYKHSKFDDNEQVLSHLGLNENQVLKTVLVDDGPTWLTFQLKTAQDVLNLNPDFSSLANFSATSNITGIDVFGVHEDGSYEARNFAPADGVNEDPVCGSGAGAVGSVLAEEYGITGEIKIKQGCKVKRNGQLKVVIEQVEGKYQVNVGGKAVTCINGTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYF1</accession>
    <name>A0A1E3NYF1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Succinate-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="8">1.2.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_45566</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 8">
            <text>succinate semialdehyde + NAD(+) + H2O = succinate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13217"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 8">
            <text>succinate semialdehyde + NADP(+) + H2O = succinate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13213"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid degradation; 4-aminobutanoate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.16" evidence="8"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037332.1">
        <property type="nucleotide sequence ID" value="XM_019182589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYF1"/>
    <dbReference type="STRING" id="683960.A0A1E3NYF1"/>
    <dbReference type="GeneID" id="30199835"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="UniPathway" id="UPA00733"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036243">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006540">
        <property type="term" value="P:gamma-aminobutyrate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07103">
        <property type="entry name" value="ALDH_F5_SSADH_GabD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000004">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000005">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010102">
        <property type="entry name" value="Succ_semiAld_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01780">
        <property type="entry name" value="SSADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF5">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="23"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00052698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="491" mass="53452" checksum="B437053C35A1F431" modified="2017-01-18" version="1">MVTPTFKDPEIVRKGAFVNGEWLTQGPNQFNVTDPATLKVIATLPDQDVSIVDTAIDAAYEAFKTYKHTTPRQRATWLRNLYNLMTEYNEDLGKLVTWENGKALPEAKGEVKYGASYFEWYAEEAPRIYGHTIQPGNPTNRAFTLRQPVGVCGIITPWNFPSAMITRKAGAALAAGCTVVIKPDSQTPLSALALAYLAEKAGFPKGVFNVVLSDTQTPEFGLKLCESSKVKKISFTGSTNVGKILMKQSASTLKKLSFELGGNAPVIVFKDADLDNAVEQAVASKFRGLGQTCVCANRIYVEKDILQKFGAKFAEKVSNFKIGHGLNEETTHGCLINSKALNKVEEHVQDAIDKGAKILLKGGRREDLGENFYEPTVLTNVDQSMKVASEETFGPLGALIPFENAKQVVEWANDVNVGLAAYVFSKDINTIYSVSEALESGMVSCNTGLFTDCSIPFGGVKESGFGREGSLYGIEDYTVIKTVNIGTIQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ17</accession>
    <name>A0A1E3NZ17_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_80527</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037590.1">
        <property type="nucleotide sequence ID" value="XM_019185080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ17"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ17"/>
    <dbReference type="GeneID" id="30202326"/>
    <dbReference type="OrthoDB" id="416217at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052400">
        <property type="entry name" value="Zn2-C6_fungal_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47657">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN ECM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47657:SF7">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN ECM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="24"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="785" mass="90308" checksum="7D136F1F61EF1D26" modified="2017-01-18" version="1">MSDTDQKEPKKVLHRKKHKNSRLGCPQCKQRRIKCDETLPSCKKCSTRAFRCGYLDYTPQELNIIRQAHMNDDELYQHAMGPMTQLINQQNSFMQIQMQPNQMQSFPQQQQQGSLSSDQQMTYPPPPQQQQVPQPFYRPVNPPQLFPQHPVYLPNQLAEFQRQPNQLSDTQQYEQLNQYNDIQEVHSGSEYGGGQYQPLPMQRGLEGHSSSTSTSELSHPIQIKSSMAPIIQITGEPPTTVDSSTTQAGTSSISSFSSKNSPFNGDDLDRSNNMELYTKDLGHSPSNSVQSGNPNEITLIRHQLNEPQFDLSKFPEFFDETIPNAFARVFLLKEVHSRLQLATSSKTLFYGMLLQGVGKLSVTIPELEAVFLKYRGKCMNTIRNQLSDIQDSNAEQLLILTSVLNSSSIYIENITMKDFFSLASGPAALLKTAFEDPSKFQKSFIKLKNHADGLLFTARSVWNPRYNHRGLFEFLEVVKEFGAKYIEDEDFYNDDDVIKVQYKNLVDFIEFALNFLEGEKSNKHVLLYPVNKIYKLVQLWYQKVPSEIYTFGSATPPVEKVFYVFWFALGEIIEDILVGGRYIFTFLFNGFYLLFPFNKHHLYQNLEDEDLRKHANYLCRIIAFLNRRKAYIVRNTILNDPIPSFFDSVDRFKPRALDIHERCITSFKNTNIKPYHYPSESTTSGNLINGTNYYKTQTDFEDGNLAAQLFSGLNTSLDAEPVAEETIEVDLLDVDPKTGLLNDDYDPRFNDPIFQSNVLFQSADLDFLIKYHEDRKLIIQLDMED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ54</accession>
    <name>A0A1E3NZ54_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcriptional coactivator p15 (PC4) C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_64572</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transcriptional coactivator PC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037640.1">
        <property type="nucleotide sequence ID" value="XM_019183764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ54"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ54"/>
    <dbReference type="GeneID" id="30201010"/>
    <dbReference type="OrthoDB" id="2505440at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.31.10">
        <property type="entry name" value="Transcriptional Coactivator Pc4, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003173">
        <property type="entry name" value="PC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009044">
        <property type="entry name" value="ssDNA-bd_transcriptional_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045125">
        <property type="entry name" value="Sub1/Tcp4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13215">
        <property type="entry name" value="RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02229">
        <property type="entry name" value="PC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54447">
        <property type="entry name" value="ssDNA-binding transcriptional regulator domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcriptional coactivator p15 (PC4) C-terminal" evidence="8">
        <location>
            <begin position="32"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="94"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="138" mass="15506" checksum="87DC8856C021977D" modified="2017-01-18" version="1">MAFKRGVKRELESAEAPSSLGSSNPDNTTVAQLGKNKRVSVRKFKKVNLVDIREYYEADGEMRPGKKGISLTEDQWWLLIDNFDAINEALTKLGGGSATKKPKLSNEFVTEEEAADDVKKEIKEDVKKEVKDEESEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ84</accession>
    <name>A0A1E3NZ84_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein DOM34 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_34202</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the Dom34-Hbs1 complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway (PubMed:20890290). In the Dom34-Hbs1 complex, dom34 recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following ribosome-binding, the Dom34-Hbs1 complex promotes the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037575.1">
        <property type="nucleotide sequence ID" value="XM_019182164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ84"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ84"/>
    <dbReference type="GeneID" id="30199410"/>
    <dbReference type="OrthoDB" id="10249111at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990533">
        <property type="term" value="C:Dom34-Hbs1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000004">
        <property type="entry name" value="Protein DOM34 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.870:FF:000001">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000008">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="entry name" value="Pelota, domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058547">
        <property type="entry name" value="Pelota_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="TF_pelota"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PELOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853:SF0">
        <property type="entry name" value="PROTEIN PELOTA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26356">
        <property type="entry name" value="Pelota_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="Dom34/Pelota N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0469">Meiosis</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ58368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="384" mass="43411" checksum="8B5E66496070B656" modified="2017-01-18" version="1">MKLINNHFEKDSSGSVTLVAEDKEDLFTLYNLIQRRDEVELKTMRNIKKSGKDGSKGSKAKTEKKLLRLKIDVEEIDFTPQDEVMRLRGKTTEPVEDVPVGTYHTAEIDFKHPFTLYKDDWDEIAFELINKSCSIDEKAEVGAVVLQEGVAHICLITENMTVLRQKIERSIPRKKRGDSSAHDKSLDKFYELVSNTLVRDLNISKLKAIILASPGFTANGLFDKIFHIATLNNDKLLLQARSKFIVAHSSTGFLQGLDEVLKTEEIQKQLSTTKFAKDVLLLDEFFKTLNADDGKAWYGPKECEKAINMGAVKNLLLTDSLFRSDDIAERKKYIELSEIVKNTGGEVTIFSSLHESGIQLDQVTGVAVILNYPVYDLDEDEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ89</accession>
    <name>A0A1E3NZ89_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">2Fe-2S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_64471</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adrenodoxin/putidaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037541.1">
        <property type="nucleotide sequence ID" value="XM_019183743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ89"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ89"/>
    <dbReference type="GeneID" id="30200989"/>
    <dbReference type="OrthoDB" id="268593at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016653">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140647">
        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000013">
        <property type="entry name" value="Adrenodoxin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018298">
        <property type="entry name" value="Adrenodoxin_Fe-S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426:SF65">
        <property type="entry name" value="FERREDOXIN-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="FERREDOXIN/ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00355">
        <property type="entry name" value="ADRENODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00814">
        <property type="entry name" value="ADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="11">
        <location>
            <begin position="54"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ58334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="165" mass="18292" checksum="803C8880B370245E" modified="2017-01-18" version="1">MAPIRSSIWHSSRLNQSMIQRISLANNSSHIRNFNTSKVQQHGHIHKPKAGEELHITFITKEGEQLTYEVAEGDTVLDIAQANNLDMEGACGGSCACSTCHVIVDPEFYDEIPEPDDDENDMLDLAFGLTETSRLGCQIKMTKEIDGIRVALPAMTRNLQSSDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZI2</accession>
    <name>A0A1E3NZI2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_56698</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MSOX/MTOX family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037299.1">
        <property type="nucleotide sequence ID" value="XM_019182787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZI2"/>
    <dbReference type="STRING" id="683960.A0A1E3NZI2"/>
    <dbReference type="GeneID" id="30200033"/>
    <dbReference type="OrthoDB" id="2219495at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050031">
        <property type="term" value="F:L-pipecolate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004657">
        <property type="term" value="F:proline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008115">
        <property type="term" value="F:sarcosine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045170">
        <property type="entry name" value="MTOX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961">
        <property type="entry name" value="PEROXISOMAL SARCOSINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961:SF46">
        <property type="entry name" value="PEROXISOMAL SARCOSINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="6">
        <location>
            <begin position="7"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="453" mass="51455" checksum="F5F1285507511789" modified="2017-01-18" version="1">MSIPSEVIIIGTGVFGISTAISLAKRYPSTKIHVFDRYEPPVADATSVDTTRVLRTDYNDEIYEKLSYEAMQLIKADPEISQFFHESGMSVIYDGKDDRWAKMYDGDIKSAQRILKNEPEKLVEFNDSTAVFKSIHGSENEPESLTKLGRKTWWNKGYCNKANGFIDAARAIEAYYKRAKTFPNIKFTFNPVEKIIFEDNTNRACGVQLQDGSMFFSKLVIVAAGAWSAKLVDLEGICFSSAIEVAWYKLSKKEEEQWKDMAITTNLSTGINIFPPYNGEIKILRRSAGYKNTIEIPHPDPTKAGSKIKISHPRTILTNKNDYIPEEAEIALRENMKEILPSLYNRPFDRTKLCWLTQTKSANFLIDYHPKLQNVLLATAGSAHGWKFVSIIGDKVVDFMSGKLDSVLKQRWSWNEKLELQDDNGSAPRMTGEAHELRDYVRDGSKLRQKANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZR3</accession>
    <name>A0A1E3NZR3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_79243</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037903.1">
        <property type="nucleotide sequence ID" value="XM_019184861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZR3"/>
    <dbReference type="STRING" id="683960.A0A1E3NZR3"/>
    <dbReference type="GeneID" id="30202107"/>
    <dbReference type="OrthoDB" id="292964at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="611"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="163"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="447"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="485"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="567"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="830"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="522"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="567"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="830"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="841"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="873"/>
            <end position="893"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1027" mass="118255" checksum="68616165A2ED5837" modified="2017-01-18" version="1">MQGYDPNASLGYTPEISEELLSLIIDLYKTYVKPTVNDLKLQKMIDLIEVAEHEFEKYQTYTIKNEYKAALNHYIVGLFYVYLIIPNSIQFQIKNKNFEIFIFLKALYDKEINMENVKTETANYISYLNEKFEMNKIELIRTRSKRSNTLPSSNLHQDLNSLLNESTTNPDERVSSSSSSTNSSIIEDDLWSPPDLQPNDQLKLVSDNYSNESLNKIPELPSHPPPPPPPSSATTGTDDQPHEPFVLHHANTLPLDFQPLSYQPKDYDIPEDGFTKSRRRQDSYHSVYVNGHEEDDLNLPMIDSKDLFGELKTDDTFWLMFDLRKKESFKINHIGYENIINIDPELLNDIEDFQNLFDVLQTVLHNDDFVKFKNLRKFEKVVIYTDNKNFLSFEFDYLVKFKNLLDEDNLQNLQLLKGGFDSWLKFISKNQSGSKVLKFKARSLPTNNHNLPPSVPPPSPPKQDSSVVAPYDYSKFLPQYSTNGIGSNQTAAAITPKKPPPPPPHYHYHHQPMNHFPVHHYPQQQNYSRSPQSIPSIPENYQYQQPYMTPPLPQQFYQQHEIQHYPQQQYSSSSSYSSDGVDLHRPNNRPRTDSVPTLQKSVNPFVRLSITGLRNMGNTCYINSMIQCLFATTKFTEIFINNKFEDYINPKFNKIKISQSLAKLFQKMYLNGGCSIVPSGFLKNCVQIRPDFKIPNEQQDTQEFLMFLLDQLHDELSNSNNVVNDYPELLTSHGGKFGEDYNKWFDKLINQGLSPISKIFQGQLKDSLKCLNCGFESSNYSTFYMLSLVIPKISAYGKKLKKIQLEDCIKLFTNEEILTGENAWDCPKCSDKNKKKDDVSSHSNSSTLSLPDQNQPRKHRLHFGNGFKFRNRSSSPSPSTSKKPSSISSNGSGKHSKKSSKSIKSLNFVTLPPILIIHLSRFLFYDLSTKDTSIVQYPLILELPSPEDSNIMIRYKLFGVVNHYGTLKSGHYTSITNKSINHDLKNPNWYYFDDEVVKTTDHGDLNKKSSSHMSSSDVYVLFYERIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P067</accession>
    <name>A0A1E3P067_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein BFR2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_31987</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AATF family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037952.1">
        <property type="nucleotide sequence ID" value="XM_019181906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P067"/>
    <dbReference type="STRING" id="683960.A0A1E3P067"/>
    <dbReference type="GeneID" id="30199152"/>
    <dbReference type="OrthoDB" id="5783963at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025160">
        <property type="entry name" value="AATF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039223">
        <property type="entry name" value="AATF/Bfr2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012617">
        <property type="entry name" value="AATF_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565">
        <property type="entry name" value="AATF PROTEIN APOPTOSIS ANTAGONIZING TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565:SF0">
        <property type="entry name" value="PROTEIN AATF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13339">
        <property type="entry name" value="AATF-Che1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08164">
        <property type="entry name" value="TRAUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AATF leucine zipper-containing" evidence="5">
        <location>
            <begin position="164"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Apoptosis-antagonizing transcription factor C-terminal" evidence="4">
        <location>
            <begin position="369"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="15"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="452"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="62"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="93"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="453"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="479" mass="54872" checksum="0318F5A3BA1A8923" modified="2017-01-18" version="1">MAKKSLAEQIAELQKPEVEDYDIEDAERDVFNHDDEENASLGEDEEDEVKRTEHYVNVGKSKLRDHSVNLKDPKYTGKTSSRKDIFENKSEDELQSENEEEEGDESDSGISLRADSEDEEQSESESEPSTDEETVTSKRDKLKQMMATERKTILNRLSTSAQNDALKGYAVISQQRLFDNILDSRIKLQKGLTNSNLLPLNNESFEEFQEESTQEDLGNVENSLHSLLDNIISLRTKICNKEKITKDQLKFNTGKKRSFGEYVEETEKLDNVLNKYRGSVLTKWSHKVQSASGATALNASKFKTINQTPAQQVDLNLMDMDRLLKRTRLNRRNVKPLGYADEEDGEENTKKVDRSLQEDENIFDDEDFYRVLLNDLIDKKISDSNPTNGLTIQLTKAKVKKNVDTKASKGRKLKYTVQEPLQNFEAPKSKFKWSDEQIDEFFAGLLGQRVNFDEADENGDNEEDEDDESLLQDDIKIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P071</accession>
    <name>A0A1E3P071_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_64480</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037549.1">
        <property type="nucleotide sequence ID" value="XM_019183746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P071"/>
    <dbReference type="STRING" id="683960.A0A1E3P071"/>
    <dbReference type="GeneID" id="30200992"/>
    <dbReference type="OrthoDB" id="1405595at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF18">
        <property type="entry name" value="NICOTINATE CATABOLISM CLUSTER-SPECIFIC TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="26"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="109"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="807" mass="92019" checksum="B6A59D42D9158800" modified="2017-01-18" version="1">MSTTVMEQYTQPQEEATDYVRIGKRFGCDIDGCDKNFARRDHLDRHKLNHNQRRLKCSRCESEFARKDLLSRHLKRHEQKDEEAGGKGMGILLTRKRVWDPKIGYTSRKRKKAAESISPKTTSTPNDEAESLPSILPSPNVESAGTNNNSLDEETTSHPVAHTRSFSSSSAENIFAHEGFIDTAHSFNLPFTTLNDYSWIFEDTPFLKEFNSSVPLGFPLINQNKDIHGSVSHKLSSAWVDSAENYNFDSDYYAEPIIPVLDNETRARLVKFIIKSTVQDGVSLFSEKDEILQPNTLQECVTSYFTKFNITYPLIHCISFNPSQVNSLLLASIILIGASYGSKKVHQSAVKIHDCLRGILFSSGHFSPSPELWVLQALLLIEVFGKSRAGLKQHEMAHLFHGLLINLIRRSGCESATTECYGPVDENDPEKSWRSWIHMESMKRLALMTFLWDVQHATFFSQTLCMSAFELKVDLPSDLNLWNSRDSTEWLGYRRSETHPLPYLSTLKKFLNCAESLPYINSLSRVLILHGLMGIAWDMQRRDQTSLNFQIDPSQWKLKLNKAYDHWKKDFDKFSRRIMLKISTNFENTTNKTFFVKYLTTNNAMYHTAVLCLWTNLIDLQIFAGSKVILGMSVNDSTFLKSKRQLTEWVYQIGISDKAVMNAAQLLRCGLTNLENWEVDGTFHYPWCLYLAVLTCWCFFKILKDQSGNKYTAKEDTPATSDDQIKGIDGVVEPIISDTKTSMRKFVSLVTSGTPDEIRSMKNIYSSDLSGMIDEIGSHLMTVRWAIIYEAFKVLKALNEDGDDGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0F2</accession>
    <name>A0A1E3P0F2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_57201</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat SEC13 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037584.1">
        <property type="nucleotide sequence ID" value="XM_019182793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0F2"/>
    <dbReference type="STRING" id="683960.A0A1E3P0F2"/>
    <dbReference type="GeneID" id="30200039"/>
    <dbReference type="OrthoDB" id="5566198at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000578">
        <property type="entry name" value="Nucleoporin seh1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037363">
        <property type="entry name" value="Sec13/Seh1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN SEC13 / SEH1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024:SF3">
        <property type="entry name" value="NUCLEOPORIN SEH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4 12" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="51"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="284"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ58377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="337" mass="37776" checksum="A1DB4D9F40BC9610" modified="2017-01-18" version="1">MKPFSTQHEELIHDVSYDFYGKQIATCSSDQHIKVFDLDTATNEWILNDSWKAHHSSIVKIDWASPEFGKVIASVSHDKTVKIWEEDLDEQRGSGRRWKHLATLNDARGPLYDVAFAPSHLGLRLSAIGSDGVLRIYDALEPADLRSWTLTSEVSVLSVPPASHLQSDFALAWCSSRFSPEKLVVCALDQGFIYHRDASGKLVQAATLPDHHGLIRSVSWAPSMGRFYQLLATGCKDGKVRIFKLTERLAQNKELAEYNEDEDENGDSKKTSNVEIQVELVSEHDDHKGEVWSVSWNLTGTILSSSGDDGKVRLWKASYSNEFQCLSVISAEQKEIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0J3</accession>
    <name>A0A1E3P0J3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Purine permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_33279</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038045.1">
        <property type="nucleotide sequence ID" value="XM_019182052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0J3"/>
    <dbReference type="STRING" id="683960.A0A1E3P0J3"/>
    <dbReference type="GeneID" id="30199298"/>
    <dbReference type="OrthoDB" id="1641903at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042907">
        <property type="term" value="F:xanthine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006042">
        <property type="entry name" value="Xan_ur_permease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00801">
        <property type="entry name" value="ncs2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42810">
        <property type="entry name" value="PURINE PERMEASE C1399.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42810:SF2">
        <property type="entry name" value="PURINE PERMEASE C1399.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="125"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="405"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="433"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="464"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="503"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="556"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="556"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="569"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="582" mass="62495" checksum="2ABEC642B69F3442" modified="2017-01-18" version="1">MGSSSAQGRTVIRKLVHKLTTKDGVLGDYDYKYLFTPQLPFMKKNKVRQPFFGLDSEMPILLGFILGLQHSLAMLAGIITPPIIVSSSANFTAQQQQYLVTASLIISGFLSMIQITRFHIYRTPYYLGSGMLSVVGSSFGVIVVVQKILPVMYKTGFCPSSDDGTPLPCPDGYGAIIGTSCVCGLLEVCFSFIPPKFLKKLFPQTVTGTVVLLMGISLIKSGFQDWVGGSGCVDGICPFEGAPKAAKFGSPQFIGLGFLVYMTIIISEKWGAPIMKSCSVILGLLVGCIVAGACGYFDKSSIDAAPAASFVWTTTFKMTVYGPAVLPMLVVYVVLVMETIGDLTATAEVSRLEVEGPLYESRIQGGVLGDGFNSIIAGLCTITPMSTFAQNNGVISITKCANRTAGYWCCCILIIMGIFTKFAASLVAIPKPVLGGMTSFLFTSVAVSGLKIISNSPFTRRDRFVLTAAILPGIGCILVPNWFENVFSYSGNNHALTGFLDAITVIMESSYALGGIIATILNLVLPQVMDDEGEDIPDDLEELALDDYVHQYDDEDKATENMKKEKSLSVKSSSNGSHSKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0N8</accession>
    <name>A0A1E3P0N8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TEL2-interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_80614</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037684.1">
        <property type="nucleotide sequence ID" value="XM_019185094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0N8"/>
    <dbReference type="STRING" id="683960.A0A1E3P0N8"/>
    <dbReference type="GeneID" id="30202340"/>
    <dbReference type="OrthoDB" id="6781668at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110078">
        <property type="term" value="C:TTT Hsp90 cochaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052587">
        <property type="entry name" value="TELO2-interacting_protein_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059075">
        <property type="entry name" value="TPR_TTI1_2nd_yeast"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057567">
        <property type="entry name" value="TPR_TTI1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057566">
        <property type="entry name" value="TPR_TTI1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016441">
        <property type="entry name" value="Tti1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049362">
        <property type="entry name" value="TTI1_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460">
        <property type="entry name" value="TEL2 INTERACTING PROTEIN 1 TTI1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460:SF3">
        <property type="entry name" value="TELO2-INTERACTING PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26245">
        <property type="entry name" value="TPR_TTI1_2nd_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24181">
        <property type="entry name" value="TPR_TTI1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24173">
        <property type="entry name" value="TPR_TTI1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21547">
        <property type="entry name" value="TTI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005250">
        <property type="entry name" value="UCP005250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TTI1 N-terminal TPR" evidence="2">
        <location>
            <begin position="10"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="TEL2-interacting protein 1 second TPR" evidence="4">
        <location>
            <begin position="391"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="TTI1 C-terminal TPR" evidence="3">
        <location>
            <begin position="797"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="791"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="796"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF26245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ58477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1043" mass="116773" checksum="12ED4467EE845EFF" modified="2017-01-18" version="1">MATDEDKRVFSEIRPYCVKLSQFTLVPPGTFDQSSIQVTEALKDVYTHLRDSVKPGATLSTALADFIFVPLSYLLKQPNLSDPQIELLLKVLTILIRHCWSPAGSIPYVLAKQLFPLITFLTGGSPNADDKSEIDVKSDELKLAGSETLQVFFNSLSKQKGAKIYDFFSNVETLPALGHAVTVLLHFCEHGKSIELQLQAIRTLCTLYFDLVADGEILSYILPGNVSSLAKVIAAPGLKTHYTVLVEAIELLGKLLVLVYNDVDLKTKVKELESIEDIMDSSEMDIITIEEADFKKVHRTNKWLKATSSQVKLALQNIKNVGPNARIEVKRALLLFCTQIIQECLYSLSSSIPVVVNILSLLSNDSRIEIDSKKLIASDSYKNQKLFDKVVSNELSTSIDKFSSVLQSPNEEKILSTIGAIDFTIRHSHDQVLASKLINFTVMELSDLLKKSLSKSKVISDSNDISNLMLITNETNDSRPDFKQLLVFSKVFSETVETKLATLFTMIGGQNDPSDLIEELLANGSDTSNYEKAVILWVSKNLLNGHFGNSRENKIVDEFLDFDEEIEKQEIPEITNAVMEFSKGMLDETSENHINQEVELINSIAVDVIGVVAYHMREDFRYELIDYLYPVVDSMASLSENVRRHALNTCMIIADTLYHGSLYEVVLDNSDYLVDAVSIRLSNAMTTRATAILAVCTKIAGFKIIESFKDVIEIIFSLLDYYHGYEDLCIGFFVLFEIIADETRKKYLHDYGVDKLEFFDSTSTFAPWGLQNVEQLKNLLDKTQRDVLVEPKGKELEEDVEGEINAPDSDDEDEQEPSAPELSEKEEKWTSPVPENIYKLLQQIMYYGERLLTHPSAKLHIQILSTYKKVLPILATSTKHLHPIVASLWPIVSKKANDSDPKIVIPAAKVVSQVLEYSGGFLSSRFVDFWEVLKSNKLLVSAKTSVSSNSKVVLPGLNSKAYEELVDMLVTGLDCLGRFISDVVAEDIVSNCIGVVQDIERFGINSDIAWSMKLEKFDINKSLSCPESFIGAHEKVYKFASIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P163</accession>
    <name>A0A1E3P163_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_63720</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038433.1">
        <property type="nucleotide sequence ID" value="XM_019183597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P163"/>
    <dbReference type="STRING" id="683960.A0A1E3P163"/>
    <dbReference type="GeneID" id="30200843"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF11">
        <property type="entry name" value="AMIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="5">
        <location>
            <begin position="76"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="3">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="230"/>
            <end position="233"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="544" mass="60459" checksum="A389F2093270D46E" modified="2017-01-18" version="1">MTVQTESWQEIAQKKREAVFAKIPKEWIIEVPSIEKVSNTRDYLDTILPKEEVAITDQSLLQLSEKIQKGELTSLEITTAFCHRAALTHQIINSCSEIFFERAFQRAKELDEIFKTTGKTIGPLHGIPISLKDQVNLEGLDSGIGLISKLNKPKTKDDESIIAKILYDAGAVFYVKTTTPMAMMAPDTNSNIYGQTVNALNRLLSAGGSSGGESSLIGAKGGLIGLSTDIGGSIRIPAAFQGLFALRPCSGRLPYAKVTNSMAFQPIVPSVIGPSARYLDDLKFFVKTIIDAKPWLYDPKTPPIPWREYEVPEKLTFGIIKNNGMITPHPPVARAMELIKSKLESLGHEVVEWEHPIPNSEMVKNLNQIFTADGYKEIKEACDESGEPIIKQLMERRAAGRELSVSEHWAQAQKKYESQLIYDKYWLDTASKTSTGRPIDAWISPLWESTSYPAGETKSYHCSYTYPVNYMDLSSMVVPITSVDKSIDKPYDNFTPISENDSLAQGVYDADLFDGMPVCVQVVTPRYEEERAISLAGVVYDATH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1P1</accession>
    <name>A0A1E3P1P1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U3 small nucleolar RNA-associated protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_79843</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
            <location evidence="1 5">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the NRAP family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038532.1">
        <property type="nucleotide sequence ID" value="XM_019184961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1P1"/>
    <dbReference type="STRING" id="683960.A0A1E3P1P1"/>
    <dbReference type="GeneID" id="30202207"/>
    <dbReference type="OrthoDB" id="10251401at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005554">
        <property type="entry name" value="NOL6/Upt22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035082">
        <property type="entry name" value="Nrap_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035367">
        <property type="entry name" value="Nrap_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035368">
        <property type="entry name" value="Nrap_D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035369">
        <property type="entry name" value="Nrap_D4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035370">
        <property type="entry name" value="Nrap_D5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035371">
        <property type="entry name" value="Nrap_D6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972">
        <property type="entry name" value="NUCLEOLAR RNA-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03813">
        <property type="entry name" value="Nrap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17403">
        <property type="entry name" value="Nrap_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17404">
        <property type="entry name" value="Nrap_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17405">
        <property type="entry name" value="Nrap_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17406">
        <property type="entry name" value="Nrap_D5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17407">
        <property type="entry name" value="Nrap_D6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Nrap protein" evidence="7">
        <location>
            <begin position="196"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="8">
        <location>
            <begin position="348"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="9">
        <location>
            <begin position="499"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="10">
        <location>
            <begin position="666"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="11">
        <location>
            <begin position="865"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="12">
        <location>
            <begin position="1038"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1174" mass="133692" checksum="2C67D5B9CA945D6D" modified="2017-01-18" version="1">MAKRTREQEIADDAQLSKKPALSKAEIIETITKNVADAESDDDQLENESIEEEESEEDEDEEEESTKDSKPKNKSTLSARDVQLAREASELFISNAFKLQIDELIKEVKLKDTKVAKIEKFLHKLYDLIQTVPELKDQTLEEAESWFKNKKTAIPYNDPKPTNINYKFGYEKPENMSIVGSFGLKTAIQSPHGLPIDVNITMPKELFQAKDYLNYRALHKRSFYLGYLTENLKTIFKREGLDFLKLSYTYLNGDILTPVLKVQATNKNLESEYNFFKTKFSINILVGFPFGVFDPKKLLPNKNCIRVQKNEELDLPATPLYNASILTMSCHDHYLKYLYKAKKSAEFFKEACILGRLWLFQRGLDSTMDAGGFGHFEFAALTAALLNGGGSQGDKVLLHGLSSYQLFKGVIKYIAEQDLQSDGYLQFHSDSNDTSKYVKGGFNTPTIFDKTTKINILHKVTRNSYNLLVQHAKKTLALLNDNIKDRFDVLFLKSSNVDFLKYDFTVQFNLSHNDDPSFAPFEKITFLTYENYIANKISSALRFGLSDRVRGINVTFDRVWDFDTSKRKPSSQNELAIKIGILVDPQESEKLVTRGPSDSEDSSQFRNFWGPKSSLRRFKDGAITHSVIWSSDYKEPIVLAIIKYILKTHIRDDLKIETNVTNVNKLLPLPNLPSSTKQPVISTNSFNSVLRSYDSLYKILIKTELPLAVKSILPISPALRYTSFLQPVPFAVSNNDFYNDLILQFETSSKWPDEISALEKVKTAFLLKISEILTEETAYKSYLTKDEDLIPYNFDVTTLNILSPEGYGFRLRVLTEKDEELYLRAVKNASKEKKQALESIYLKFAQNYQGLVIHTRTISTLAHQFQFYSPTVRLFKKWLDDQLLLSHLSEELVELIALQPFIDPAQFDVPASVENGFLRILHFISHWNWKEDPLILDVSKRIDDDESNVLSKLSDRLTLASYQQIQSNFKKVRGEDPQGLRTQFFVATKADPSGIMWSNNSSLPIATRLTALSKVAIQLINSNGLNERTLNLLFTPSLGDYDFVVKLKTIPLTVSSGVLPNTSAFKNLLNTSNSYPSDITSKFNPILKLVKELNLKFQGSIIFSHHQHTIKETGENVITGLFIPNKLQTSKFKVQLGYNFEPVSKDEVIINKQAIFNEVLNVGGDLITGFDSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1Y3</accession>
    <name>A0A1E3P1Y3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamine amidotransferase type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_32368</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038706.1">
        <property type="nucleotide sequence ID" value="XM_019181947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1Y3"/>
    <dbReference type="STRING" id="683960.A0A1E3P1Y3"/>
    <dbReference type="GeneID" id="30199193"/>
    <dbReference type="OrthoDB" id="10252281at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051857">
        <property type="entry name" value="Asn_synthetase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937:SF1">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="3 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="8">
        <location>
            <begin position="2"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="403"/>
            <end position="404"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="525" mass="59715" checksum="15EB7B1D5CE3067C" modified="2017-01-18" version="1">MCGILFHYNQNQALENGVLEFQETQVLDIIKNGLGSKIPNSSQIFNSLVPKICSRGPNYSSLLVENNNMLFFSSVLSLRSPFTKQPLESSRYIIQFNGELYNNDIDGNDTQYIMNLLDQKSVIDTIHTLDGEFAFTIYDKKEQNICFGRDPVGKRSLAYYLKNEELYISSVQPGFEERSDFIDCDNGSIYQYSFKNKHLEIVEHAVTYNINSNTDKDFKNIEDRLNKLNNVLIQSIKQRIHNIDPFHIESETDPLYAILFSGGLDCTVIAGLAASISKPETSIDLFNVGFDNPRTGMKASEAPDRKLAIESWLNLSKQFPSINFNLIEIDIPYEEYLETRPKVIELMYPKNTEMDLSIAIAFYFASRGKGNRLSLHDVPLEQISYSSAKTVEHYQSKAKVLLSGLGADELYGGYHKFANKDNESLAEELTRQINNIHERNLQRDDKVISDNGVEVRYPFLSHDVIQFSTQEIEINYKISKLILRKLASQIGLEFVSEEPKRAIQFGAKSAKMTANGNKKGTDELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2K0</accession>
    <name>A0A1E3P2K0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">THIF-type NAD/FAD binding fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_78196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038755.1">
        <property type="nucleotide sequence ID" value="XM_019184681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2K0"/>
    <dbReference type="STRING" id="683960.A0A1E3P2K0"/>
    <dbReference type="GeneID" id="30201927"/>
    <dbReference type="OrthoDB" id="1708823at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF162">
        <property type="entry name" value="SUMO-ACTIVATING ENZYME SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="1">
        <location>
            <begin position="12"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="338" mass="37906" checksum="8E55D3E813A7496C" modified="2017-01-18" version="1">MSELTADELALYDRQLRVWGADGQNKIKNASILVINLNGVGTEIVKNLTLSGVGSIELSDPSIVSEQDLTAQFFLSEEDLGKSKLEAVEPRIKDMNPRVNLTINTTPINYDDSEYFKKFQLVIANNLEPQSFQKLNNITRELNISLYSTVAHGLYGYFFSDLILDVSNFTKKKMPIARKAGPISKNQEILKISSSHNKETNEISETFTLKSKFSKFEDVLHSKNLNDLTRKGKKNVSPLLSIFFSLYELGLEGLEISIDVLKTRANEINKRLGLNDIVDESYFTKVTTQLNTEISPVAAILGGTIAQDVINYISKKEIPINNLLILDGDNFTMPIYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2N7</accession>
    <name>A0A1E3P2N7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">mRNA cap guanine-N(7) methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">mRNA (guanine-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">mRNA cap methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_30550</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Responsible for methylating the 5'-cap structure of mRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:67008"/>
            <dbReference type="Rhea" id="RHEA-COMP:17166"/>
            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:167617"/>
            <dbReference type="EC" id="2.1.1.56"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.56" evidence="3"/>
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        <property type="protein sequence ID" value="ODQ59746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_019181743.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="30198989"/>
    <dbReference type="OrthoDB" id="10248867at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="C:P-TEFb-cap methyltransferase complex"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="F:mRNA 5'-cap (guanine-N7-)-methyltransferase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="mRNA cap guanine-N7 methyltransferase"/>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="mRNA_G-N7_MeTrfase_dom"/>
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        <property type="entry name" value="RG7MT1"/>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
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        <property type="entry name" value="MRNA CAP GUANINE-N7 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12189">
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="RNA_CAP0_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="9 16" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
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            <begin position="47"/>
            <end position="324"/>
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        <location>
            <begin position="56"/>
            <end position="57"/>
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        <ligand>
            <name>mRNA</name>
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        <location>
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        <location>
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        <location>
            <position position="128"/>
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        <ligand>
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        </ligand>
    </feature>
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        <location>
            <position position="154"/>
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        <ligand>
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        </ligand>
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            <position position="159"/>
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        <ligand>
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    </evidence>
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    </evidence>
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    <sequence length="326" mass="37591" checksum="64C7323E8EAC3F97" modified="2017-01-18" version="1">MERERETVVEQKIQEEITRKKENLDQIVRQHYNQRAYVSKHSRRHLSPIIKLRNFNNAIKYMLIGNFSQPGWRALDLGCGKGGDINKWSMANISEYIGIDISNASIVEAIKRYNNLQAGFETTFITGDCFGQPLPFILKDFPHIHLPVDIVSMQFCMHYAFETEIKARTLLENVSRSLRAGGYFIGTIPSSDFITEKVAKLPPGEKKFGNSIYSVTFENDPPRDGNFRPAFGNMYTYFLRDAIDNVPEYVVPFEALRALAEEFDLELTYKKTFLEMFKEEIPTWFRKLNPRLVEGMRRTDGTYGVEGDESEASAFYLAFAFQKAAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2Q8</accession>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
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                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="62" mass="6993" checksum="1EE1E0E37BA88A07" modified="2017-01-18" version="1">MSAWRKAGFSFNKYLQISAQTVRKALKPEFQTQKVQARGNTDAKVIKFENGVAKEAEPLAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P316</accession>
    <name>A0A1E3P316_WICAA</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_62460</name>
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    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
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        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
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            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039083.1">
        <property type="nucleotide sequence ID" value="XM_019183375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P316"/>
    <dbReference type="STRING" id="683960.A0A1E3P316"/>
    <dbReference type="GeneID" id="30200621"/>
    <dbReference type="OrthoDB" id="18139at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012098">
        <property type="entry name" value="SND3_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28112">
        <property type="entry name" value="SRP-INDEPENDENT TARGETING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28112:SF1">
        <property type="entry name" value="SRP-INDEPENDENT TARGETING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10032">
        <property type="entry name" value="Pho88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008756">
        <property type="entry name" value="P_tr_PHO88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="190" mass="20942" checksum="05B526564A962402" modified="2017-01-18" version="1">MNPAVSNLALMLVMMQVSRKLNFEDPTVVFYVRVAFATATIVTLGVYFYVKSIIIKKNDLTTLKFVTPPNAMAGETEEKLEVTTVKDYDLKQVQAAIKGVYTNLAMMGFMHLYMKYTNPLVMQSISPVKSAFESNIVQIHLFGKPASGDLKRPFKAASMFGGFGGQDSVKTDKHSIEQAEKAGNGGVKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P336</accession>
    <name>A0A1E3P336_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aminotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_29752</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039032.1">
        <property type="nucleotide sequence ID" value="XM_019181643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P336"/>
    <dbReference type="STRING" id="683960.A0A1E3P336"/>
    <dbReference type="GeneID" id="30198889"/>
    <dbReference type="OrthoDB" id="10261433at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43094">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43094:SF1">
        <property type="entry name" value="AMINOTRANSFERASE CLASS-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="455" mass="50175" checksum="3784F00779F73FEB" modified="2017-01-18" version="1">MTIEQSAILQTSIKQLPVAVSGKGVYITVKDPKTGQLKELLDGSSGAAVSSVGHGDEEIKQKMIEAVQDQIYTFPILTSNEQSEKLARFLIANSKEGAFETATFTGSGSESNENMLKLVRKYHIENGDFKRIKFISRNQSYHGFTIGALSIADNFRKREFQDILMPESITPKALQCYPYRFKNKSESLEQYKDRLLQNLEDVFQKEGPDTIGAFIAETVVGSTFGCNPPIPGYLDGCKAICEKYGALFVLDEVMCGMGRVGSFNAWEQFMTTGGPDIQTIGKTLGAGYVTIAGILVSPKVMKVFRENNAQIAGAQTYHSHSFNCQVSLAVMEKVVRDGLIDNIKEQGNYLGAQLAEVLKDSKYVGDVRGKGGFWAIEFVKDKETKEPFPADVKFSYKFAEKLYDNGVFCLNGNGTVDGILGDHALFSPAYSITKEEVDLIVSAVQKTVDELEKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P370</accession>
    <name>A0A1E3P370_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SIT4 phosphatase-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_22395</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPS family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039133.1">
        <property type="nucleotide sequence ID" value="XM_019180944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P370"/>
    <dbReference type="STRING" id="683960.A0A1E3P370"/>
    <dbReference type="GeneID" id="30198190"/>
    <dbReference type="OrthoDB" id="295029at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007587">
        <property type="entry name" value="SAPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634:SF8">
        <property type="entry name" value="FIERY MOUNTAIN, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634">
        <property type="entry name" value="SIT4 YEAST -ASSOCIATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04499">
        <property type="entry name" value="SAPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="80"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="451"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="703"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="455"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="498"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="706"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="790" mass="89512" checksum="FB3C37C50B6D248B" modified="2017-01-18" version="1" fragment="single">SFWRFGNGYNEVSTINNLLDNGFESFAPNLEILGDLLDEQEILEELASSNIKLIEYLRQREVLEKLIEYIITEEDDLVDDSQKNEKNDDDSNNNNNDNNDNEEDESKSESSDYKRRKSLNSNSDSDSDDVQNYDQPETIQEIHSRRAQVAAEILSADAWSLTDSFMDNHDLLSKLWSVLDKPCPLSMVLSTYFMKINEHLLDMKIDEMLNFLLHQNNLVDRFLKHIDNPPLMDFLLKVISTDKQDIPTGIIDLLKSQNLVSKLISFLDPKVNASVQSAAGDFIKAFVTISANSNTDNSTIGPNELTRELVSEKIMNQLCELMLKGGTSLANGVGIIIEIIRKNNSDYDFVPVLFITIESHPPTSRDPIYLGHLIKVFSKNIPKFASLLKDTKLPPMDTPFGSIEPLGFERFKICELIAELLHCSNMGLLNDEKGEEIVRERDIERERVIALEKQQDDEEGEEGEGAQNEAGDEDSDHEASKVIKRLSISSTEQDNEEDKTTEKEEVKEKDVTTDDDKDSTPTENKPSLEIQSEPDSDADTTLEIQYDEHETQIRKNPVVGDQLKIALADNQVILVILNMFFCFPWNNFLHNVVFDIVQQILNGSMDIGYNKYLAIDLFGRGEINKLIIEGQQKCEDYEQTNGLRLGYMGHLTLIAEEVVKFTALYPPNTIHQIVYDAVSTPEWTVYVTETLVDTREKYNALLGGSVEDEGGDETTEDNITPGEVDDDYNTTGNNRSLDSHEEDDEEGDNNRGNNESDDVEGDQFSRYMSQQLTNDLPDKFGSSDEDEEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P377</accession>
    <name>A0A1E3P377_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 75</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_32980</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038559.1">
        <property type="nucleotide sequence ID" value="XM_019182020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P377"/>
    <dbReference type="STRING" id="683960.A0A1E3P377"/>
    <dbReference type="GeneID" id="30199266"/>
    <dbReference type="OrthoDB" id="19419at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070775">
        <property type="term" value="C:H3 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010698">
        <property type="term" value="F:acetyltransferase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="188"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="212"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="26367" checksum="5217DC235C6C2B0D" modified="2017-01-18" version="1" fragment="single">SEIARLKSVQEIYRKRSEHTKKIDRFWHIVLSQHPEFSEYIRSSDFKYLELIKDIYVTWDVSQPESESSNPGDFSITFEFNALGDDFPAQIIVKNDDEDNADDTERLVSENADIVWPESYDSINPVKIQDKKTPEGKKNYRTGMKSFFGWFKWTGRKPAKEFPNGEDFARLFSEDIFPYAVKYYTEAQRDGLEEEDDSDASEPLDLSDNDDDSRGTKRKHEEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3K7</accession>
    <name>A0A1E3P3K7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_91778</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Golgi apparatus membrane</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039047.1">
        <property type="nucleotide sequence ID" value="XM_019186322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3K7"/>
    <dbReference type="STRING" id="683960.A0A1E3P3K7"/>
    <dbReference type="GeneID" id="30203568"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000067">
        <property type="entry name" value="ras-related protein RABA1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="187"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="24420" checksum="B1E2BEE7CE2CB772" modified="2017-01-18" version="1">MAETDDYTYDYEYLFKIVLIGDSGVGKSNLLSRFTRNEFNLESRSTIGVEFATRTVEIEGKRVKAQIWDTAGQERYRAITSAYYRGAVGALLVYDISKSESYENVSKWLKELKEHADENIVIELVGNKSDLDHLRAVPTEEAKQFSAENQLLFTEASALNAENVDLSFQQLLKSIYDMVSKHQFDLSDYSGPKPSGGPTISLTPTPGNESSKNKSNCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3Z7</accession>
    <name>A0A1E3P3Z7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alcohol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_52965</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038814.1">
        <property type="nucleotide sequence ID" value="XM_019182703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3Z7"/>
    <dbReference type="SMR" id="A0A1E3P3Z7"/>
    <dbReference type="STRING" id="683960.A0A1E3P3Z7"/>
    <dbReference type="GeneID" id="30199949"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000947">
        <property type="term" value="P:amino acid catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08297">
        <property type="entry name" value="CAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000002">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="10">
        <location>
            <begin position="16"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="348" mass="36754" checksum="FE6189EE7F6B0F8C" modified="2017-01-18" version="1">MSIPKTQKGVIFYESGGELQYKDIPVPTPKANEILINVKYSGVCHTDLHAWKGDWPLPTKLPLVGGHEGAGVVVAKGANVTNFEIGDYAGIKWLNGSCQSCEYCEKSFESNCPKADLSGYTHDGSFQQYATADAVQAAKIPKDADLAEVAPILCAGVTVYKALKTADLDAGQWVAVSGAGGGLGTLAIQYAKALGLRVLAIDGGDEKGELVKSLGAEVYIDFTKTKDIVKDIIEATNGGPHGVINVSVSEKAIEQSTEYVRPCGTVVLVGLPAGAVARAQVFGAVVKSVSIKGSYVGNRADTREAVDFFTRGLVKSPIKIIGLSELPKVYQLMAEGKILGRYVLDTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P455</accession>
    <name>A0A1E3P455_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_54071</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039476.1">
        <property type="nucleotide sequence ID" value="XM_019182731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P455"/>
    <dbReference type="STRING" id="683960.A0A1E3P455"/>
    <dbReference type="GeneID" id="30199977"/>
    <dbReference type="OrthoDB" id="2017974at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005697">
        <property type="term" value="C:telomerase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045153">
        <property type="entry name" value="Est1/Ebs1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696">
        <property type="entry name" value="SMG-7 SUPPRESSOR WITH MORPHOLOGICAL EFFECT ON GENITALIA PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696:SF0">
        <property type="entry name" value="TELOMERASE-BINDING PROTEIN EST1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="2">
        <location>
            <begin position="663"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="604"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="604"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="837" mass="95794" checksum="98C4F047E49D313A" modified="2017-01-18" version="1">MTELWDIYNTNITLLNSYYDFLLYSLSSGRSGKQIVQVYRIPRRLWVYGIVTFLDVLKNVVSIFIEHDICSSFIGYSFNILSGLTDLEMEGWVSEKLGDLSRMAIALYPSRYIDWKISSEFWYTTALKTQYGFGKIYYHIATVQQDNLDALVNIGKSVFCRDTFVPTPQYMRMVIDNINQRNFVDLPVVDFIKVHKILLSSEFNSDLELIKLVSYYSQNIGIDNNKVDFFVRTKGVVNEHIESPGYNQDNDYENKLNFWFQRSSSFALVNICQVVGFGNSANPFARLFGLVEALKERKDRKEKKDKRKKSVSNGDQQQDQANGTRSIDIDSEDSISSKDLSVEEWFQASDYINKNALELAMRMFANYLNGPIITSTPHVITYLYFIISIGEALRETPTAASFFEFLLHAILPRKPLVVYLNEVLDHVRKHKPEIIDKLEELRPSIMSEGNFINYFNKNENLTEVWKCWGTLWYDQIQIKEDFANFRDAGIQRDDFLDVPIGGVRYNKELNNDRYTRILLLASYIADEFNFGLKRGVDGFQFAEYAFENTILRSDVIKGTVHNFLRDSRIQGLNSPIPETLLYEKSSSANITSDVISWLRSKSSSARTSNDSNQSGGISYVTDADNEADYDEEEDDREQLLSNQIGDDNRVLGNIGIGMDSSLSFFVLDTNMWLKHCGKIFKSVRSHIFRLAVPLVVFQELRSLRRSPDGNIADAAVRAVITVRQLASEGCVLALKLNGTEAPSLNDIKDFENNSTWMNSIDDSIIESSRLLDINANNLNRAKNAEAGNNTNTTISFSILVTEDRNMRLKARTKLVPAYQAKWFFQILEKVSNGRCND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4B7</accession>
    <name>A0A1E3P4B7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">L-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="19">1.1.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Cytochrome b2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Flavocytochrome b2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">L-lactate ferricytochrome c oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">WICANDRAFT_31465</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(S)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + pyruvate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:19909"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.1.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:19910"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">In the C-terminal section; belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">In the N-terminal section; belongs to the cytochrome b5 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.3" evidence="19"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039307.1">
        <property type="nucleotide sequence ID" value="XM_019181835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4B7"/>
    <dbReference type="STRING" id="683960.A0A1E3P4B7"/>
    <dbReference type="GeneID" id="30199081"/>
    <dbReference type="OrthoDB" id="1925334at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004460">
        <property type="term" value="F:L-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02922">
        <property type="entry name" value="FCB2_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000009">
        <property type="entry name" value="Cytochrome b2, mitochondrial, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000062">
        <property type="entry name" value="Cytochrome b2, mitochondrial, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037458">
        <property type="entry name" value="L-MDH/L-LDH_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF148">
        <property type="entry name" value="L-LACTATE DEHYDROGENASE (CYTOCHROME)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="24">
        <location>
            <begin position="80"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="25">
        <location>
            <begin position="182"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00061589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00066458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00068515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00075949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00078774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00078938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="ODQ60100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="573" mass="64172" checksum="83E8AB8A57DD731A" modified="2017-01-18" version="1">MFKGQLRTATARSSFRSLASKLNPQRFNSSKTPLLNATRGSNRSKNSLIALAISLSAVSSSYYLYQKDKFISADVPHWKDIELTPEIVSQHNKKDDLWVVLNGQVYDLTDFLPNHPGGQKIIIRYAGKDATKIFVPIHPPDTIEKFIPPEKHLGPLVGEFEQEEEELSDEEIDRLERIERKPPLSQMINLHDFETIARQILPPPALAYYCSAADDEVTLRENHNAYHRIFFNPKILIDVKDVDISTEFFGEKTSAPFYISATALAKLGHPEGEVAIAKGAGREDVVQMISTLASCSFDEIADARIPGQQQWYQLYVNADRSITEKAVRHAEERGMKGLFITVDAPSLGRREKDMKMKFEADSDVQGDDEDIDRSQGASRALSSFIDPSLSWKDIAFIKSITKMPIVIKGVQRKEDVLLAAEHGLQGVVLSNHGGRQLDYTRAPVEVLAEVMPILKERGLDQKIDIFVDGGVRRGTDVLKALCLGAKGVGLGRPFLYAMSSYGDKGVTKAIQLLKDEIEMNMRLLGVNKIEELTPELLDTRSIHNRAVPVAKDYLYEQNYQRMSGAEFRPGIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4D4</accession>
    <name>A0A1E3P4D4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Spo7-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_20592</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038934.1">
        <property type="nucleotide sequence ID" value="XM_019180878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4D4"/>
    <dbReference type="STRING" id="683960.A0A1E3P4D4"/>
    <dbReference type="GeneID" id="30198124"/>
    <dbReference type="OrthoDB" id="5599171at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071595">
        <property type="term" value="C:Nem1-Spo7 phosphatase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071072">
        <property type="term" value="P:negative regulation of phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005605">
        <property type="entry name" value="Spo7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28249">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN SPO7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28249:SF1">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN SPO7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03907">
        <property type="entry name" value="Spo7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="27542" checksum="CAE38003ED5B3D54" modified="2017-01-18" version="1" fragment="single">SSRRRKSDSSERSNRSVRSSSIHKDKISPTTKIFRNLLILEESLRQQSQDQRSLRRKFTAFLAILTGLAGFAFYSLYFSDINPSQLVKVMLQFMFFFILITLVLFNLSGEYRRTIIIPRRFFTSTNKGLRQLNLRLVKVKSPITDRIIDWARAIMMSIVQFDQQVLDHTGFFKSTKAGKWVKKNLRILEIRAQPRVGATDVKLVLNPRAFNAEIREAWELYRDEFWAREGSRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4D8</accession>
    <name>A0A1E3P4D8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_62633</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039271.1">
        <property type="nucleotide sequence ID" value="XM_019183408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4D8"/>
    <dbReference type="STRING" id="683960.A0A1E3P4D8"/>
    <dbReference type="GeneID" id="30200654"/>
    <dbReference type="OrthoDB" id="3979490at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="406"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="517"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="26"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="543"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="549"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="565" mass="62623" checksum="7DE9BFA78688F235" modified="2017-01-18" version="1">MGFSITETLSKGRNVVPMEKRNKTLAYSLVLIATCLDNLNVAGTMTSVFSVAERFDASTTTVSWVFSSYALTLGAFIIIAGKLADVWGPHNVFLVGLFFTSLFALISAVIVDQIIVLIVFRALQGVFAASLIPSAFSLTGNYFKGDALLKALAWFIMSLTVVFGIGVILGGAFSVSSVGYKGLYYFTFAVGMACFIALYFIIIPIEKTEGHQNLKIENLDYGGSAFLVIGLLLINLGLTEGGESWHKASSYVPLVVGVLVFFGVVIFEMWYIQGFKDKVNSQINNIEGNTTHFGNEQIKENQDDVCNGSIKSTSEFSGTNDWRYKIELLFPREVIKIPNFFQYLFATFLFYINYIAVMSTVVQYSQYIELDSPIMAALKVLPLALGVTFAAVTYRQNLAQRVGTKFIICCTPILVLTMSVWISRLDFRVKNSYWKYECIGQFIFGFGTNIYFQVYWSDIITGTPLHLQGVVSGILQTAGQIGLCFGNTIIAALAGELKYTRDYDTRVELHHKFKKNFYLAYAGSAALFFVLLTVKGKKSVAAKDDVENQPKNQDLETNQDTSNAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4H2</accession>
    <name>A0A1E3P4H2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_62889</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039532.1">
        <property type="nucleotide sequence ID" value="XM_019183455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4H2"/>
    <dbReference type="STRING" id="683960.A0A1E3P4H2"/>
    <dbReference type="GeneID" id="30200701"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="8"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="313" mass="34840" checksum="4AB7150798DE2BE4" modified="2017-01-18" version="1">MAAIGETVLLTGANGFIALHILGKLLERGYKVIGTVRSQEKADALYKKFETDHSDPNLKLVVVEDLTAAEIKYVLHTASPFKYGFTDNYDEGYRKPAVQGTLEVLHSIHKYGHKVVTVVVTSSFAAIKHTDKLKDPTFIHNENVWNPDEWDNAKTENQAYSLSKKLAEKAAWDFIKKNDVKFHLDTITPPFVFGPQYFSQDAANDELNTSNQIIVDLLKSDPKDTKFFDGHTLAAVDVRDVAEFHILAFERGIKNHRLFPSGVPINDQKILNIINEYIPQLNGKVAKGNPEKAYTVPGVGLSSDTSKMLLGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4N7</accession>
    <name>A0A1E3P4N7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Complex 1 LYR protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_84200</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I LYR family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039520.1">
        <property type="nucleotide sequence ID" value="XM_019185662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4N7"/>
    <dbReference type="STRING" id="683960.A0A1E3P4N7"/>
    <dbReference type="GeneID" id="30202908"/>
    <dbReference type="OrthoDB" id="14535at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20266">
        <property type="entry name" value="Complex1_LYR_NDUFA6_LYRM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045299">
        <property type="entry name" value="Complex1_LYR_NDUFA6_LYRM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016488">
        <property type="entry name" value="NADH_Ub_cplx-1_asu_su-6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12964:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12964">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B14 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="9">
        <location>
            <begin position="23"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ60313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="126" mass="14848" checksum="ED321B041F996A8B" modified="2017-01-18" version="1">MPTLPTAFAETTKRSLNSGELSQRVLSLYRKYLRSAPVFIELYELDLPVGVVRTKIRQEFERNRFQKDININNVLLAKGQMEYQELINFWKQTPHVMRYFEFEGDTRFSKDAKIADSFVGKFLKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4T9</accession>
    <name>A0A1E3P4T9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_84251</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039580.1">
        <property type="nucleotide sequence ID" value="XM_019185676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4T9"/>
    <dbReference type="STRING" id="683960.A0A1E3P4T9"/>
    <dbReference type="GeneID" id="30202922"/>
    <dbReference type="OrthoDB" id="6910977at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051061">
        <property type="entry name" value="Zinc_finger_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF13">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="148"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="176"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="197"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="219"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="249" mass="29236" checksum="070E468A97BB5EDC" modified="2017-01-18" version="1">MTTEQNIVHNDHEDNEHQSQKRAKPNPIIEKELNLSIIHSEELSKFIPNDFPTTIDENGRIPLRRQIFNDKKVLRTVLTKPKKGVYMCNHCDKIFNTFGELLDHFDEFNVKRPHKCSFDDCPWKIVGFNRIRQLHRHEKSVHSTEKDFKCNVPNCNKKFGRVDLLNRHIKSVHENKTSRFNKKMSKEQQQQQQQQQPSSDYNSSNDELTSGGEYSPVVSSSSIDQQSQQQRRGSQYKHTIKFLTNSDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P510</accession>
    <name>A0A1E3P510_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GDP-mannose transporter</fullName>
            <shortName evidence="5">GMT</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_30155</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the import of GDP-mannose from the cytoplasm into the Golgi lumen.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homooligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Golgi apparatus membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TPT transporter family. SLC35D subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039625.1">
        <property type="nucleotide sequence ID" value="XM_019181692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P510"/>
    <dbReference type="STRING" id="683960.A0A1E3P510"/>
    <dbReference type="GeneID" id="30198938"/>
    <dbReference type="OrthoDB" id="417037at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00803">
        <property type="entry name" value="nst"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="128"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="316" mass="34526" checksum="3ACBC479E446B936" modified="2017-01-18" version="1">MSQAPQVQAGGLASLVNNGPVSILCYCISSILMTVTNKYVVNGFQFNMNFVLLLVQSLVCVVAIAILKTFGIINYRPLNKTDAKNWAPISILLVVMIYTSSKALQFLTIPVYTIFKNLTIILIAYGEVLFFGGQVTSMALGSFILMVLSSVIACFGDQKDNTSFALSIGYFWMALNCFSSASFVLVMRKRIKLTNFKDFDTMYYNNVLSIPILLLSSFILEDWSPANLNANFNKPSIIAMIVSGLASVGISYCSGWCVRVTSSTTYSMVGALNKLPIALSGLIFFDAAVNFLSISSIFIGFLAGIVYAVAKQQQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P535</accession>
    <name>A0A1E3P535_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">YbgI/family dinuclear metal center protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_53535</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase I type 2/NIF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039154.1">
        <property type="nucleotide sequence ID" value="XM_019182713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P535"/>
    <dbReference type="STRING" id="683960.A0A1E3P535"/>
    <dbReference type="GeneID" id="30199959"/>
    <dbReference type="OrthoDB" id="3345469at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1390.30:FF:000001">
        <property type="entry name" value="GTP cyclohydrolase 1 type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.30">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002678">
        <property type="entry name" value="DUF34/NIF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036069">
        <property type="entry name" value="DUF34/NIF3_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00486">
        <property type="entry name" value="YbgI_SA1388"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799">
        <property type="entry name" value="NGG1 INTERACTING FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799:SF13">
        <property type="entry name" value="NIF3-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01784">
        <property type="entry name" value="DUF34_NIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102705">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR602678-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="29929" checksum="65BEFCE10DFE27E9" modified="2017-01-18" version="1">MSLKRAVNAIQKLYPLRYADSTWDNTGLLVDSSSSTIATSSSNTSILLTIDLTKAVVEEAINQKVQLIIAYHPFIFRGIKKIEPFANPQHDSLIKLISNGISVYSPHTAIDAARGGVNDWLIEGVTSIDNVESKEIIEPNSEDPQNVGMGRYITLSNPIELKDIVASVKHHLGLQHLQLATNDINAPIKTVAICAGSGGGVFNQLKGKPVDLYLTGELSHHEVLSLKESGSSVIAAHHSNTERGYLKIVQQQLQKELADTKIIVSTKDSDPYKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5F5</accession>
    <name>A0A1E3P5F5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pre-mRNA-splicing factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_78758</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in spliceosome maturation and the first step of pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the SPP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039349.1">
        <property type="nucleotide sequence ID" value="XM_019184783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5F5"/>
    <dbReference type="STRING" id="683960.A0A1E3P5F5"/>
    <dbReference type="GeneID" id="30202029"/>
    <dbReference type="OrthoDB" id="5577072at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045166">
        <property type="entry name" value="Spp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026822">
        <property type="entry name" value="Spp2/MOS2_G-patch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15818">
        <property type="entry name" value="G PATCH AND KOW-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15818:SF2">
        <property type="entry name" value="G-PATCH DOMAIN AND KOW MOTIFS-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12656">
        <property type="entry name" value="G-patch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Spp2/MOS2 G-patch" evidence="6">
        <location>
            <begin position="154"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="230"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="256"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="268" mass="30266" checksum="8691B7F392DE9B08" modified="2017-01-18" version="1">MVGFSLKKKDEGSKQGFSGFKLKKDTNNLPKKSNKPFGFAKEEDEEVKEQKIDTFDTNEGGAFNKEEGPITKEGPLVIENEGNHQWRNKAQQRFNPTAKPTTQESENSKLEYGINYLEKSADSKATDDSGNPSTPIHSHEQETLSFKEDVKSRPEMATMEDYERIPVESFGAAMLRGMGWKGEQNEEKDENKKAVIPVAQRTLYLGLGAKDNKSDGGLKPIDKTYVPVKMINRKTGEVHTEEEKRPNSRNRERSPVRSSSSNSYRSRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5L7</accession>
    <name>A0A1E3P5L7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sec1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_82986</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039825.1">
        <property type="nucleotide sequence ID" value="XM_019185468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5L7"/>
    <dbReference type="STRING" id="683960.A0A1E3P5L7"/>
    <dbReference type="GeneID" id="30202714"/>
    <dbReference type="OrthoDB" id="2228at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031340">
        <property type="term" value="P:positive regulation of vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="645"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="653"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="682"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="692" mass="80136" checksum="C6E198BE80C89FA0" modified="2017-01-18" version="1">MTSLIELQRNRILNLIRDVRTENGYKILVLDETTEELVFSVLNKSEILNIVTAIERLDQKRNNQFSIEAVYLLEPTKYTINCLLTDFTNIPTRYKAAHIFFLPGLTTELSNKLKANIHFQKNLRTFQEFYLNIHSKESNLFVTKNPNSLQVFYNSSCHDLVTKTIIHTARALVDLCVITGEYPIIRYYSPNDNDGYFNASVLPKMIATDLQEQLDEYTRTHPDFPPATTRQRSIFIITDRTLDLFAPLLHEFTYQAMTYDIVNNQIKNDVYTYEAENEAGMKEEKQSKLDDSDPEWVQLRHLHILDAQELLTSKTEEFLSKNSMLVDRSKIKTTSDILHAVAHLKGFDEERRRITLHKTLLETLMLTNGEKKLAEIAEFEQNVANFGVDIDGERVKNLADLLIDYLSQDVYPFADKLRAIILYGLYRGGLIEQDYIKLFNFMGVLQLHEIDYNLELIKNFETVGFKLIKPNLKSKSVFRREFLHESVSENSYNTSRFKPSINSIITKVLSNTLDDERFPYIKDKPMDLENDISRTNSTSTASLKNPKHKASWAKTNTQFQPPRQRIFYFIAGGATYSEARTAYELSNKFEKDIIIGSDELITPSQFIAEVKKLSLERPNLKLYADWKAGKPTEAPRYLLDNYSEKRPNDVHASANSQVRQQPTKGKPSAQLTKAEEPEKEKKRSKFKKFLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5N5</accession>
    <name>A0A1E3P5N5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_61157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039797.1">
        <property type="nucleotide sequence ID" value="XM_019183119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5N5"/>
    <dbReference type="GeneID" id="30200365"/>
    <dbReference type="OrthoDB" id="10406305at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="181" mass="21694" checksum="FA77882C27C89C56" modified="2017-01-18" version="1">MDKNELDPKVCKILDSNIQLQHHYDFLLNKKRIADKALKEFITRVENDYADEITKISEQKTDPTSIVDYTQAMDFIRNVTLKNASKLEEIQKYITQQQELEKVWEYTHHRLINDSKTDDDTLNAFYNECMRQLEEFSKKCHQKFHALEIPLFCLKKELQYKNLSKDRRNLIAFIQDEAMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5Q3</accession>
    <name>A0A1E3P5Q3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_27815</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039781.1">
        <property type="nucleotide sequence ID" value="XM_019181415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5Q3"/>
    <dbReference type="STRING" id="683960.A0A1E3P5Q3"/>
    <dbReference type="GeneID" id="30198661"/>
    <dbReference type="OrthoDB" id="10256055at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41677:SF1">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41677">
        <property type="entry name" value="YALI0B19030P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="44636" checksum="7D0440076449DCE8" modified="2017-01-18" version="1">MAVAVLPSSLTPDVLHGNHSFDIPSDVYDEQVLPLLKELEKIGIIDPSSIKFEPSKHIEFTDEYFQTTKKITLEELGVENPHQTPISPIGVSHPFPLFTEEAILIMRWEMFQKDTFLKCARVADHSNTGDLDMYLRGFVKDYAPFTYQAWNHPRTMEIISKMAGVELEHMFDYEIANSNISIRNNQTKILDETKDEYEDPNDIPAILSWHFDSPQFVCVLMMSPTDDMIGGETSLTRGDGKVVKVENPKRGWANVLQGRILKHVAPKPRGNYSERITQVCSYRPSDPMKDNCAATTIKPSQLAGTRYNEFYKDWMNYRLDVLNDRIKVLQDQINTTTAKGETFSQIETINFIQNNIVEYAEHSWQEFEVVDDGLVQKPASYKVTQARWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5R6</accession>
    <name>A0A1E3P5R6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="7">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_13183</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="7">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="7"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039875.1">
        <property type="nucleotide sequence ID" value="XM_019180563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5R6"/>
    <dbReference type="STRING" id="683960.A0A1E3P5R6"/>
    <dbReference type="GeneID" id="30197809"/>
    <dbReference type="OrthoDB" id="193716at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070013">
        <property type="term" value="C:intracellular organelle lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="19"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="238"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ60668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="452" mass="50040" checksum="5787CF86CCA0A86E" modified="2017-01-18" version="1" fragment="single">ALTKLNFENFTPVQSKTIGPLTVGEKGIIARAKTGTGKTLAFGLPLIEKALKRDPRSKDVVGLIISPTRDLAYQIRDELNKVVRSFDRKTPHINIHCLVGAEPKFGQIKAFTRHQSFPHIVVATPGRLYDMMENPMIQRALAGIEFKVLDEADRLLDDGFKDSLLEIETAIKDAKAQNNGKQEKTLLFSATIDRNVMTFAEDLMGNDLNYIDCVDKDAPETHEKVEQSLISTNNLFESFTSACLFIENEAKANKNSKVVVFLPTVKCVSYFKETLAQYLNTNNVRTPVFELHGKLSQSQRDRSVRQFRERSKGVLVTSDVGARGMDFPGVTNVVQIGVPTDTTNYVHRIGRTARGGKSGQALLILTKAEGPFVKVLLDRHINIAKQYAYEKIEESEAAITEASVGAIHKFSPDDAIGSLCGFYNGVTRNYRLNLKSVLADITGAYSNLVGEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P647</accession>
    <name>A0A1E3P647_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA mismatch repair protein MSH6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DNA mismatch repair protein Msh6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_20456</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the post-replicative DNA mismatch repair system (MMR).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040040.1">
        <property type="nucleotide sequence ID" value="XM_019180873.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P647"/>
    <dbReference type="STRING" id="683960.A0A1E3P647"/>
    <dbReference type="GeneID" id="30198119"/>
    <dbReference type="OrthoDB" id="10252754at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032137">
        <property type="term" value="F:guanine/thymine mispair binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032138">
        <property type="term" value="F:single base insertion or deletion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043570">
        <property type="term" value="P:maintenance of DNA repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000710">
        <property type="term" value="P:meiotic mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000019">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.10:FF:000002">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000771">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003810">
        <property type="entry name" value="PRK05399.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF148">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="13">
        <location>
            <begin position="918"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="56"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="106"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="120"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1094"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ60833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1094" mass="122766" checksum="F4EBC9E55A929A42" modified="2017-01-18" version="1" fragment="single">SSPTKRQKKQISYAESSDEDEGEVTISKTQRNKRRRVIKDDDASEDEFVPDAKNESEDDHMDDDFIVPDDFEEDKAVEIDDDDDFIEITKKSKPKAKTPPRKATSSLANKFTSGSTYKATSKSPSTTPKPKAVVAAKQTKSQKFTKENEERYQWLVNILDAEKRPESDPEYDPRTLYIPQNAWGSFTNFEKQYWEIKSKMWDCIVFFKKGKFYELYEKDAMIAHNEFDLKIAGGGRANMQLAGIPEMSFDFWATSFITKGYKVAKVDQIETGLAKEIREANGAPKGKKDVIQRELKCVLTAGTLTDETMLSDDMATYCIAVKEDIDPVDEAGKIFGVAFIDTSTGHIQMTEFKDDSECTKLETLASQLRPKELIISKNNLSQLAIRILKFNAQNNAIFNYIKPDSEFFDFDTTFETLTRGKYFEAENLDDLSKWPMTLKEYYESNKMVGFSAFGGLLWYLKSLKLDENLISLGNITPYTTIKASTNLVLDGQTLQNLEIFANSFDGTDKGTLFKLINRATSPFGKRLLKTWVVHPLLQKKDIESRLDSVDQLLEDGDLRSTIESSLAKLPDLERLLSRVHASQLKLKDFTRVIEGFEDIAKLYKRLGSFELKGALAKVYSKFPSEIDECLSKWEDAFDRVQAKDHGVLLLSKGIEQDFDDSQETIADLEHALNTKLREYRRQFKSQAIDYKDSGKELYTIEMPANIKVPNDWKKMGASKKMTRYWSPEVEGLAKSIARARESHKLIEESLIERMSKRFDSDYSVWINAINAIGKMDCLVALAKTSESIGFPATRPTFIDSETGVLDFKELRHPCFNMGVSSSKEFIPNDVTLGGDAPNLGLLTGANAAGKSTVLRMTCIAVILAQIGCYVPCETATMTPVDRIMTRLGANDNIMQGKSTFYVELSETKRILENSTPKSLLVLDELGRGGSSSDGFAIAEAVLHHVATHIQSLGFFATHYGSLGQSFKSHPQVKPFRMAIIVDEGSKNITFLYKLEDGAAAGSFGMHVAAMCGIDKEIIKNSEVAAEEFEHTSKLKKNESNYLNLIPLGLQSDFARLLKEGFRNSSDGVGEGVLIYNETVQKKALQSIGQLIDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P648</accession>
    <name>A0A1E3P648_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">H/ACA ribonucleoprotein complex subunit CBF5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">H/ACA ribonucleoprotein complex subunit cbf5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_104080</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>uridine in 5S rRNA = pseudouridine in 5S rRNA</text>
            <dbReference type="Rhea" id="RHEA:47036"/>
            <dbReference type="Rhea" id="RHEA-COMP:11730"/>
            <dbReference type="Rhea" id="RHEA-COMP:11731"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>uridine in snRNA = pseudouridine in snRNA</text>
            <dbReference type="Rhea" id="RHEA:51124"/>
            <dbReference type="Rhea" id="RHEA-COMP:12891"/>
            <dbReference type="Rhea" id="RHEA-COMP:12892"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pseudouridine synthase TruB family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040122.1">
        <property type="nucleotide sequence ID" value="XM_019180395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P648"/>
    <dbReference type="STRING" id="683960.A0A1E3P648"/>
    <dbReference type="GeneID" id="30197641"/>
    <dbReference type="OrthoDB" id="10250002at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000495">
        <property type="term" value="P:box H/ACA sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000454">
        <property type="term" value="P:snoRNA guided rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02572">
        <property type="entry name" value="PseudoU_synth_hDyskerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21148">
        <property type="entry name" value="PUA_Cbf5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000001">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012960">
        <property type="entry name" value="Dyskerin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00425">
        <property type="entry name" value="CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003280">
        <property type="entry name" value="PRK04270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08068">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01136">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dyskerin-like" evidence="10">
        <location>
            <begin position="17"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="9">
        <location>
            <begin position="266"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="362"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="362"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="374"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="414"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="435"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="481" mass="53827" checksum="99E6ACB1BCB78320" modified="2017-01-18" version="1">MSEEQFIKPENVSASQDASEWPLLLKNFDKLLVRSGHYTPIPAGSSPLKRDLKSYISSGVINLDKPSNPSSHEVVAWIKRILRCEKTGHSGTLDPKVTGCLIVCIDRATRLVKSQQGAGKEYVCIVRLHDALKDEKELGRSLENLTGALFQRPPLISAVKRQLRVRTIYDSKLIEFDNKRGLGVFWASCEAGTYMRTLCVHLGMLLGVGGHMQELRRVRSGALSENDNLVTLHDVLDAQWSYDNTGDESYLRRIIQPLEGLLLGYKRIVVKDSAVNAVCYGAKLMIPGLLRYEEGIELYDEVVLITTKGEAIAIGISQMSTVDLASCDHGVVAKVKRCIMERDLYPRRWGLGPVAQKKKQLKADGKLDKYGRINENTPTDWKSNYVSHENDQPPAPTSTAATPKPLIKEVEMTDSPAASVADVSVADVTETPSKKDKKEKKDKKEKKDKKEKKDKKEKKEKKRKADDEDEEGEEKKKKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6A2</accession>
    <name>A0A1E3P6A2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">D-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="9">1.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">D-lactate ferricytochrome C oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_84253</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the stereospecific oxidation of D-lactate to pyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(R)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + pyruvate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13521"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.1.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.4" evidence="9"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039584.1">
        <property type="nucleotide sequence ID" value="XM_019185677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6A2"/>
    <dbReference type="STRING" id="683960.A0A1E3P6A2"/>
    <dbReference type="GeneID" id="30202923"/>
    <dbReference type="OrthoDB" id="7786253at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000038">
        <property type="entry name" value="D-lactate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF111">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="13">
        <location>
            <begin position="107"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ60377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="542" mass="59261" checksum="F1EF9CE8AD3A3D5D" modified="2017-01-18" version="1">MLRSRLINASRVGSRFISRTQQRSIHTIQNSSKSSSTSLLLATTITSAESSTTSLSELSPPKYGSKEDLAKAKEEILKIVGEDNLATSQAELESHSDTYFNSHHALDDQRPQLIVFPSSTEEVSQILKITHKYRIPIIPFSGGTSLEGHYIATTGTPSITIDLAKNMNNIIALHKEDLDVVVQPGVGWEDLHDYLTDKGLLFGPDPGPGALIGGMISTSCSGTNAARYGTMKENVAGLTVVLADGTIIKTKNRPRKSSAGYNLTGLIIGSEGTLGIVTEATLKLHVKPKEETVAVVSFDTISDASNTVADIVQHGIQVNAVELLDDKMMQVVNESGETTRKWIEKPTLFFKIGANNQSVLTELIKEVNLISQSNKSSNFEFAKNADEIEELWSARKMALWSTINKGRQEHPDMQIWTTDVAVPISKLANVLSETKSDMAKSGLTATLVGHAGDGNFHAFLLYRPEQRSIAEGLVANMVKRAIEAEGTCTGEHGVGYGKREFLLEELGEDPINMMRRIKMSLDPLRLLNPDKIFKIDPNDHEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6G1</accession>
    <name>A0A1E3P6G1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_40652</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040223.1">
        <property type="nucleotide sequence ID" value="XM_019182555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6G1"/>
    <dbReference type="STRING" id="683960.A0A1E3P6G1"/>
    <dbReference type="GeneID" id="30199801"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044283">
        <property type="term" value="P:small molecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209:SF812">
        <property type="entry name" value="2OG-FE(II) OXYGENASE FAMILY, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G14880)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209">
        <property type="entry name" value="OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="7">
        <location>
            <begin position="192"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="395" mass="45230" checksum="BC79381941D27FF2" modified="2017-01-18" version="1">MTTSDVTDKFSELKVSSGESPSSIPTLDLNKATNPETKSEFLDELRNALFNFGFFYIKNPPFDVEKFKNIKEESLKFFEIPLEEKKKVAMVNSKHFLGYNGLGDEITAANVDWREQVEFATELPEPDIKNDDELYKNIEGPNLWPNGDFLPEFKHIVTDYIDELSKLSRQFTHWIEDALGLEYGALEAFFKEVQQAKLKIIKYPDLANLNEQVEAVEKEFANTQYKTKLNQGVGEHRDNDFLTFIYQASHHTSLQVKDFNGEWINVPPVENTLVFAVGLTLEFITQGVCVSTVHRVLTPTPGEGDRLSISFFQTINIESKKTSIEVDQKAKDLVKLRDAKRTKNKIGFQFQVDETKPVGYSVFLNRVKSHPKVGERHYPKVLKYIQEEVAKASDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6I2</accession>
    <name>A0A1E3P6I2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycerol-3-phosphate dehydrogenase [NAD(+)]</fullName>
            <ecNumber evidence="9">1.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_90121</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 9">
            <text>sn-glycerol 3-phosphate + NAD(+) = dihydroxyacetone phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.8" evidence="9"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040071.1">
        <property type="nucleotide sequence ID" value="XM_019186211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6I2"/>
    <dbReference type="STRING" id="683960.A0A1E3P6I2"/>
    <dbReference type="GeneID" id="30203457"/>
    <dbReference type="OrthoDB" id="10263760at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141152">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000004">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000294">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006168">
        <property type="entry name" value="G3P_DH_NAD-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006109">
        <property type="entry name" value="G3P_DH_NAD-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017751">
        <property type="entry name" value="G3P_DH_NAD-dep_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011128">
        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03376">
        <property type="entry name" value="glycerol3P_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728:SF8">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(+)]-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07479">
        <property type="entry name" value="NAD_Gly3P_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000114">
        <property type="entry name" value="Glycerol-3-P_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00077">
        <property type="entry name" value="GPDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00957">
        <property type="entry name" value="NAD_G3PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0520">NAD</keyword>
    <keyword evidence="2 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent N-terminal" evidence="10">
        <location>
            <begin position="30"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent C-terminal" evidence="11">
        <location>
            <begin position="229"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="35"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="306"/>
            <end position="307"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ60864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="387" mass="42294" checksum="0F7A52020CC14BD3" modified="2017-01-18" version="1">MSSASQRLSQLGNHLTSNRKASLAPEKPYKITVIGSGNWGTTIAKVVAENAALRPHLFQRRVDMWVFEEQVNGQNLTEIINTNHENVKYLPGITLPSNLVANPDLVNAAEGADLLIFNIPHQFLPRIIKTLQGANLKPHTRAISCLKGLEVTPEGCELLSTYITEHLKVHCGALSGANLAPEVAKEKWSETTVAYTIPNDFQGQGKDIDRLVLKAAFHRPYFHVRVIEDVVGVSLAGALKNVVALGVGFVHGVQWGDNAAAAIQRFGLNETIKFAEIFFPGETNSDTFTKESAGVADLITTCAGGRNVRVAKHMAITGKSAQEVEKELLNGQSAQGVITAKEVHELLDNKGLTKEFPLFEAIYQIVYGTESIERLPELIEENEDLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P755</accession>
    <name>A0A1E3P755_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation factor Tu</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_61815</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">G-protein that, in its active GTP-bound form, binds to and delivers aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. In the presence of a correct codon-anticodon match between the aminoacyl-tRNA and the A-site codon of the ribosome-bound mRNA, the ribosome acts as a GTPase activator and the GTP is hydrolyzed. The inactive GDP-bound form leaves the ribosome and must be recycled before binding another molecule of aminoacyl-tRNA. Required for mitochondrial protein biosynthesis and maintenance of mitochondrial DNA.</text>
    </comment>
    <comment type="function">
        <text evidence="10">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040462.1">
        <property type="nucleotide sequence ID" value="XM_019183250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P755"/>
    <dbReference type="STRING" id="683960.A0A1E3P755"/>
    <dbReference type="GeneID" id="30200496"/>
    <dbReference type="OrthoDB" id="2067at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01884">
        <property type="entry name" value="EF_Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03697">
        <property type="entry name" value="EFTU_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03707">
        <property type="entry name" value="EFTU_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000001">
        <property type="entry name" value="Elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000003">
        <property type="entry name" value="Elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_B">
        <property type="entry name" value="EF_Tu_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041709">
        <property type="entry name" value="EF-Tu_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033720">
        <property type="entry name" value="EFTU_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004541">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_bac/org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00485">
        <property type="entry name" value="EF-Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000766">
        <property type="entry name" value="PRK00049.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009372">
        <property type="entry name" value="PRK12735.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009373">
        <property type="entry name" value="PRK12736.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF36">
        <property type="entry name" value="ELONGATION FACTOR TU, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="42"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ61255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="432" mass="46934" checksum="EDCF26DD09AB7965" modified="2017-01-18" version="1">MFTKTLVNNARLSLRAVAAANSVAQFVRPALARSAASFNRSKPHLNIGTIGHVDHGKTTLTAAITKRLAEAGGADFLDYASIDRAPEERARGITISSAHVEYETANRHYSHVDCPGHADYIKNMITGAAQMDGAIIVVAASDGQMPQTREHLLLARQVGVQHIVVFVNKVDTIDDPEMLELVEMEMRELLSTYGFDGDNVPVVMGSALCALEGREEEIGVKAIDKLLAAVDEYIPTPQRDLEKPFLMGVEDVFSISGRGTVVTGRVERGNLKKGDEVEIVGLNKTPLKTTVTGIEMFKKELDQAMAGDNCGILLRGIKRDDIKRGMVIAKTGTISAHTKFLASMYILTKEEGGRHSGFGEHYRPQLFIRTGDVTVVLTFPEGGDSSQQILPGDNVEMVCELVHPTALEAGQRFNIREGGRTVGTGLITRIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P773</accession>
    <name>A0A1E3P773_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 6">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_77915</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FKBP-type PPIase family. FKBP2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 6"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040483.1">
        <property type="nucleotide sequence ID" value="XM_019184633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P773"/>
    <dbReference type="STRING" id="683960.A0A1E3P773"/>
    <dbReference type="GeneID" id="30201879"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005528">
        <property type="term" value="F:FK506 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000006">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044609">
        <property type="entry name" value="FKBP2/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45779">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45779:SF7">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0697">Rotamase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133735" description="peptidylprolyl isomerase" evidence="7">
        <location>
            <begin position="20"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="8">
        <location>
            <begin position="43"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="141" mass="15315" checksum="D69CEF4C54DB99F1" modified="2017-01-18" version="1">MKITSLITASLALFSFAAAEEKLQIGVTHKIPEDQCHRKARKGDTVQVHYTGTLKEDGKEFDSSLKRGDPLEFKLGTGMVIQGWDQGVAGMCIGEKRKLTIPSHLGYGKRGAGGVIPPDADLVFTVELVGIKGYENIKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P783</accession>
    <name>A0A1E3P783_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_29050</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039934.1">
        <property type="nucleotide sequence ID" value="XM_019181569.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P783"/>
    <dbReference type="GeneID" id="30198815"/>
    <dbReference type="OrthoDB" id="79452at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044879">
        <property type="term" value="P:mitotic morphogenesis checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035024">
        <property type="term" value="P:negative regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF96">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN BEM2_IPL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GEF" evidence="4">
        <location>
            <begin position="348"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="5">
        <location>
            <begin position="782"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="6">
        <location>
            <begin position="1547"/>
            <end position="1733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="103"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1383"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="22"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="103"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1383"/>
            <end position="1396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1405"/>
            <end position="1428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1748" mass="197816" checksum="3C566605AA67B3DB" modified="2017-01-18" version="1">MNTLTQNDGESISQSIHPSSSFKLNHTNTSSSSSRPFNYDTYDSTEIKTGWLNKGNHVNNWTNVNSDSWRIYKAQLKGPVLSLYKVPADVNIKAFDHSACPTSLESASSSTPSTSVRQKRLSTASSRLNMKADTKSSSSNLHSSSASSSSNSISSANTSNSSATTKNFRIKLKYLSEVYPHPDLQLNSANSIISGTLESICHTILFNTLNDDKLSYNLLVILPLFGDVKASLRYFIEYGISFSSQRQSKNKVVVSSNTDHAITQRMALVITTICDSFPGMLLDDDLKELVIKLKETISIHNDELAKQLQSLVSSKEGEMKNLTFFKNQQYQPNGSLNLTNVDDFLKLDSELLAKQINAIDLKFNKVWNPKTDASLLYELENLSYSRYNPLIFNSTNNVHYLGRLLVNHLFGDNESKNSEFKRSRILSKWIELGCFFDKIGDMVSWLAIATVICSMPILRLKKTWSLVDEHYLKTISTEWAPVVFELDRRTMISEASHRSSYHVIAPQGLGISYSKHDVVPYFGDLTVKYIENSTLKQCEKRVQRVKVSFSRWDEYLSNVEQDNDQFKNQTFNENQELTKQLYNLLTNHVQLPPLTQESIMELSLSIEANTIGQYHKLHYNSRTPLITGSYLPTLFTEVLPNYKLFDQSVLIGAGGFAAKPNDTQSEEDDSSFQKVTGVNDVDLHSYVLNSKIPSSKQHVFLKSVRDIFNIGSDVFHIDDDLIFKSLNNGDEWKSSRPASVVLENPSSKRLSQISNNRLKEEANDVFAILDNSNLLNSLIKPLNVVLKAGTFEKLIDILVLTSNVFSKKINQKDIDSYLEKSNLLDNQFLKLNMNNGVFTSTFFATYKSFATTSMLLENLGKRFIGAKSGAVSIAKLNTEGDLHGEQKVFPDWYSQVASDDENLNWKFVGKIQLGILEALSILVNEYYADFTDDLQVKKIFVDILKIIDNEIIVEWKGVLSRYEYSESKNELTEIYEVLSSLYKKIRKNYIKKCYRPLDVFPKHLPNTDQKNFGSISFPKLPSDFEEAELFVEKLDWFINDLFSSVSISDWISVFQLLEIQTGKSLTSLFKFKGTDSTSEDDLEILNVFTWIKSLYGDNMDDKILNKFPPPVKILFDVHSNLEKYFKLQISDTNISRDIRTSRMISILQVLAISRLRNGSVDLFNGSEKLEDSDIGKVSPHVPSFIETAITQAIVSPESRSFSKSWIYSALTLNSSSTADNWDDLVQLLPKFEIEHLNKSLKGALTPCVGWFVERLLEISCFVPNMSIENSKLINFDKRRFTYNCITNIVDMNANFQNTFAQSGSESDQIRDEFSFLFKLAHYPTVDHKQVKEFASRENKEYPKNDQKAKFLGGLIASEQEKIKRDLKKREVLENQERDIKRTQLLSQASRASTTIQRDVPQYNDQSSLRKPRQSSSPSKGSGSSMGKRLGGLLKSVRPFSISVGSNWSGPDRVVHPDELPDVNSVDLSGKHSKPYQQIKLFNTKPLFVHSNIEGFFKVVGENGEDYCFQALNNAEAQSWISALNLSKRYTYLSKDAQGLTSSKVFGVPIADVCEREGSLIPRIVERLLQEIEVRGLDETGLYRIPGSVGSINLLKQAFDEGGDFTLEDDRWFEINTLAGCFKSYLRELPESLLTSELLPEFVLATKQGDLVENMKSLVRQLPVHNYHLLKRLFEHLSKVIQHSENNRMDAVNLAIVFSMSFINNDNIGASMGTDLGALQTILQSLIKNPEAVFNEQYNDDSTVFTAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7A5</accession>
    <name>A0A1E3P7A5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_83091</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039964.1">
        <property type="nucleotide sequence ID" value="XM_019185488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7A5"/>
    <dbReference type="STRING" id="683960.A0A1E3P7A5"/>
    <dbReference type="GeneID" id="30202734"/>
    <dbReference type="OrthoDB" id="2985014at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17327">
        <property type="entry name" value="MFS_FEN2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000068">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000018">
        <property type="entry name" value="MFS transporter permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF24">
        <property type="entry name" value="NICOTINIC ACID PLASMA MEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="34"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="472" mass="53264" checksum="02C7E9910AB75D22" modified="2017-01-18" version="1">MSSEKELEDVKQAVSPEIDKHEEDKLVRKLDFYLMPIFCLIYFLSFLDRSNIGNARVVGLQEQLGFTDSQYSTAVSIFYATYVTVELPGTLAVKKIGPHRYLTFALVAWSLITIFTCFVRSYGSLLATRLLLGLFEGSVFPSQSLYITMTYKRNEQTKRVGYLYICSCLSGAFGGLIATGITKIKPVGSFQSWSWLYVIEGCISLLAAVWVWFGLPDDPKKARFLNEREQHLMSIRAEQTRRYQGKQEFEMKEVYAAWKDPKVWLSCCIQFCCDIVLYGFSTFLPSILKLQLGYNSMEAQYLSVPCYVMAAIGVAVISYCSDHFGHKHYLLVGGNMFAMIGYIILLSTTKGGACYFATYLIAITLYGSVGVNLTWMNINMAPHYRRAAALGTNQMLGNVAGAIAGQIYRKSPYKLGNGFSLGCVVVGSLLATVLRFHLQRENKIKEQILSGEREDHLKERTGDKDLEYKYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7E6</accession>
    <name>A0A1E3P7E6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_27731</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040542.1">
        <property type="nucleotide sequence ID" value="XM_019181404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7E6"/>
    <dbReference type="STRING" id="683960.A0A1E3P7E6"/>
    <dbReference type="GeneID" id="30198650"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000464">
        <property type="entry name" value="GTP-binding protein ypt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ61335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="218" mass="24300" checksum="D3881C43861B40E5" modified="2017-01-18" version="1">MSKSQQSSTTNSASAGSAGEPRFAQFKLVLLGESAVGKSSIVHRFVKDSFDDFRESTIGAAFLTQTIQLDESTTVKFEIWDTAGQERYKSLAPMYYRNANAAIVVYDITQESSLERAKSWIKELQRQANSDIVIALAGNKLDLENERQVSKDIAQAFANEENLLFFEVSAKSGECIKEIFTKIAEKLPLESQLKPNRLQTRGVDLQRPSTNNQRSCCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7F9</accession>
    <name>A0A1E3P7F9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Alternative oxidase</fullName>
            <ecNumber evidence="17">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">WICANDRAFT_83131</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes cyanide-resistant oxygen consumption. May increase respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 17">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="16 17">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the alternative oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="17"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040014.1">
        <property type="nucleotide sequence ID" value="XM_019185495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7F9"/>
    <dbReference type="STRING" id="683960.A0A1E3P7F9"/>
    <dbReference type="GeneID" id="30202741"/>
    <dbReference type="OrthoDB" id="16906at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098803">
        <property type="term" value="C:respiratory chain complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009916">
        <property type="term" value="F:alternative oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010230">
        <property type="term" value="P:alternative respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01053">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.140:FF:000002">
        <property type="entry name" value="Alternative oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.140">
        <property type="entry name" value="Alternative oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002680">
        <property type="entry name" value="AOX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038659">
        <property type="entry name" value="AOX_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803">
        <property type="entry name" value="ALTERNATIVE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803:SF3">
        <property type="entry name" value="ALTERNATIVE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01786">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005229">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12 16 17" id="KW-0408">Iron</keyword>
    <keyword evidence="14 17 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 17 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="325"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="325"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005229-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ60807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="350" mass="41039" checksum="78F037D29BF75A84" modified="2017-01-18" version="1">MILKTVITRSSPIKGPNTRIQPFGVRFISSTKSNQRTIFPPELSNEPQHKSKFDISTKLIINPPLEIDKDQYITHPAFPHPQLSNKDCEQVHFVHRQPISTGDKIADRGVKICRKLFDFITGYKKPDDLGNLKNHTVKGTRYEMTEEKWMTRRGVPGMVAAFVRHLHSLRLLRRDKAWIETLLDEAYNERMHLLTFMKLGNPSWFTRLIIYIGQGVFCNLFFLIYLMKPRYCHRFVGYLEEEAVSTYTHLIHDIDSGSLPKFDKVKIPEIAWLYWTELDEKSSFRDLIQRIRADESKHREVNHTLANLKQEIDRNPFALKLDGIPKKDQPDEYDLKSSRPSGWNRDQLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7P2</accession>
    <name>A0A1E3P7P2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_78008</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040585.1">
        <property type="nucleotide sequence ID" value="XM_019184648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7P2"/>
    <dbReference type="STRING" id="683960.A0A1E3P7P2"/>
    <dbReference type="GeneID" id="30201894"/>
    <dbReference type="OrthoDB" id="270602at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02958">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14346">
        <property type="entry name" value="UBA_Ubx5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006577">
        <property type="entry name" value="UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF6">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13899">
        <property type="entry name" value="Thioredoxin_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00594">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="393"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="476" mass="53383" checksum="FD2084259DCD97ED" modified="2017-01-18" version="1">MSEQVDTFLAITGSSDGKVAEHFIDMAGGDIETAISLFFEHGASAAQPSNPNTTPPSHTDDDAALAERLQNEAYQNQQANEPRAPDAAVHERLVGGEDHFGVFPGTFGGVGGSFGGLLNRPAPQDIFGNARPGIFNQRDDDFPQDDSEEEDKVVEVDSDGDVIESRRDDGMSETQRRLARIFRPPFDIIDKLDLNSAKQKARKEKKWLLVNIQDNAEFQCQVLNRDFWSNGNVKDLVKDNFIFLQYQKESRSGEEYSQFYHFSEYPHIAILDPITGERLKMWSGVPATNSWIQEVFDFLAQFSLEPGHINPTVSHKKKIDPSTLTEEQQMELAIKESLGKKEQDHDDVQILHGGEQNDPIEIDDDVDEEPKELTEQEKFQKIQAIDHPEPEANPATTTRVQIRLGDGSRQVRRFNTDDKVVVIYEVLKATVESVKNGEAFTLTSQRENLIGKLDETISDAGLKNASILLELVDKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7Q4</accession>
    <name>A0A1E3P7Q4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_90232</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040149.1">
        <property type="nucleotide sequence ID" value="XM_019186219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7Q4"/>
    <dbReference type="GeneID" id="30203465"/>
    <dbReference type="OrthoDB" id="4954565at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058353">
        <property type="entry name" value="DUF8040"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF85">
        <property type="entry name" value="GH03217P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26138">
        <property type="entry name" value="DUF8040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8040" evidence="9">
        <location>
            <begin position="49"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="275"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF26138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ60942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="550" mass="63718" checksum="33E5B242D2197187" modified="2017-01-18" version="1">MLASDEVDDLVQSVGILNDFRLFEDEERLFWTYAQAVEVIDTTSLKPRNELTSKLLLLDEHQFREEVHMTKHQFFSIYYLFSKSGLLRDGSRYGIKDKLLMAIYITANSFSYDDCLIAFGLSNKGELSRMFTQVLDAFCAIQHFIINKDIVNINVNRAENPLYQNKSQEFKEAYKIFVDVFGYDGSIGATSFSISGYGLTEDLKNGDYYSYEMGGLGISTMAIINTRGLVDYLGEQYPAAKPGYETYKMDIWSSNLDRVLAYNHESKNFDDSKILMKFGDNEYYCDQFLVVPYEGNNIRCDLLDFGFKKVSDYDYSEPDSQSVMSLQPRDRYELFNLNHSQVRVSIDQLFGAVKNRFRILNNIIEFPTKKIPQLTKFCFILHNLLTFNDLQLFTAVRNIIKNIKVTDFESGRDLDSAEDPTSSTQTRANEYFSNLVTEETKCESRVQRLRVSLMLERNYIKSFPLRLKMFNFKSYDIVYDGPVVPNFSNDEGISIQGRNHQLLFEQVLISVDNGVYKLQREKLEVLKRDIAQFTDEELTQQVQTIFNGTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7T9</accession>
    <name>A0A1E3P7T9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_41432</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040680.1">
        <property type="nucleotide sequence ID" value="XM_019182562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7T9"/>
    <dbReference type="STRING" id="683960.A0A1E3P7T9"/>
    <dbReference type="GeneID" id="30199808"/>
    <dbReference type="OrthoDB" id="504170at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000399">
        <property type="term" value="C:cellular bud neck septin structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032161">
        <property type="term" value="C:cleavage apparatus septin structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005940">
        <property type="term" value="C:septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044879">
        <property type="term" value="P:mitotic morphogenesis checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12194">
        <property type="entry name" value="Kcc4p_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14081">
        <property type="entry name" value="STKc_BRSK1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000394">
        <property type="entry name" value="Serine/threonine-protein kinase HSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.220">
        <property type="entry name" value="Fungal kinase associated-1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031850">
        <property type="entry name" value="Fungal_KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043024">
        <property type="entry name" value="KA1_sf_fungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16797">
        <property type="entry name" value="Fungal_KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="31"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="394"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="576"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="790"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="533"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="395"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="576"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="587"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="790"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="804"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1041"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ61473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1041" mass="118163" checksum="09B5966AF1642800" modified="2017-01-18" version="1" fragment="single">MSYHSRQPSVASSLLSSSTFNTSQINKIGPWKLGKTLGRGSTGRVLLAENINTNQKAAVKVVSKSILNNLESSISDEKGRDAAGLAYGIEREIIIMKLLNHPNVLRLYDVWETSKSLYLILEYVEGGELFDLLVEKGPLLEKEAIRYFTQIILGASYCHALGICHRDLKPENLLLDHDLNIKIADFGMAALENNDRLLETSCGSPHYAAPEIVSGKHYHGFESDVWSCGVILFALLTGRLPFDDENIRNLLLKVQNGVFEMPQDISIEAQDLISKMLTVEPSKRIKTHEILNHPLLLKYPLNQSDLKTLNELPSPNTYLNQINSIDEIDDQILDNLVILWHGRAKAEIIENLLKPHSTTEKIFYFLLLRYRHTQSLTSNNNSLARSLSIAKSVQNGGSVSNSSGSSKTTTPKKNRRSVISVSSSHKKPVSFNTKNRRSIKDINELNTPPVPKTIIDEFKANNVGTGITKSTSKRRSMRTSILTSKRNSITTRLISVYAKLANDNDWEYIDKEAKRTSSNFATLCDEIFDHEKYEKLRLEKEELERQLKLQEEELRKQEELERQKLEDERIAELKRQEEIEAAKEPKLQNNKIRSTSGPTPQRNSQLLDKDEISKIQQRAISHPLESRPKSKLDPGLNFNQDQKKNSKFNYYDENDSIVQAIRRSKFLGSKFDLDLKRQSISNQQKQVSKNFEDEKIVDKHENGFNGPKTIAEVKIPQVTRKSRHFSNSNKRLSVLSIYSTRNSFTNLTSLKELDNTYKPSPLIPEEAEFSTSKSKSSGLRLSYADRLTALQESQEESTPVVNPQDDKKLHADDSMYKGLNIPDLPDHYEPIKPKRKSTIKVEIHDDDEQEPEVEPKPLPKPKRSAPSPPKEINPSVSTTKVTKAPLGDVTNDLNNKRTGSSGGGFFRKWSLGKKDHEQLSSNTNNGGFFRRFSGNHSVQQKTLNTVYESTEMYQTLKRLLLGWNDYGLKNIKTNDELLLIEGEISKNNIMSLNSTRFKLNIEQYGKKGSCIKFEKLKGSTKSFKTLINEIERILNRENVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P804</accession>
    <name>A0A1E3P804_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_77986</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040561.1">
        <property type="nucleotide sequence ID" value="XM_019184646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P804"/>
    <dbReference type="GeneID" id="30201892"/>
    <dbReference type="OrthoDB" id="3982981at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="8"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="645" mass="73483" checksum="938371E13A2EC62D" modified="2017-01-18" version="1">MMLQQQEPLNLLTLPNEIFQLILYQLESSEIVEFLRSSRKVQNMLKDSYHIVTDEVKYLEGNISKELYSDVVDICPIFHEGKNTSFNYNRTFLLQIHDPKTSSETFEKLIHSLPINCRCKISKYVNTQSLGDSSFTEPIEIRAYEFRIIKSVEIVDSTTLRLQNSTWDLSLFKLPKVSKLLLNNCFIEPQHSYKVKFPNLSDLYIDGANHQVFESIELPENVALFGIDAVVENLKSTNLQDLRIINSELSLIGNCDFPKLDQFVIFDSNIGQLNKFRACSLKTISINSRHSTILWNDLHLPNLETLKIIASEISFEFSNVYCPNLIEASIYAYNQPNPEESLSTPFRFLEKLKSLTIWGGCVQILEGLKLENLESFLIDGRVQYELSQDVYFPKLSSLVLWGTSKTLLKIPKLNAPNIFKIELRGDIINLERSDLHCYPMGISLEVRIQHGVEIDAIDVSCLKRLSISYDESRELLQPTLNFKESLNSLELFRFSPILETNPKCAKSEYSSAFCNIQLLLGDDLESLQAWAYGKPVCTRVLSISKLLKMSTARNGLHSRTSDMLMPLDMGNENSGDLFCLRGVSALYFGTTFNPGHYMYEDADALFAKDPDLVKILSDHDAEMFRDFTSDQQGSMGFFGTASLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P815</accession>
    <name>A0A1E3P815_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ribosomal RNA-processing protein 44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_62074</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the RNR ribonuclease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040725.1">
        <property type="nucleotide sequence ID" value="XM_019183305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P815"/>
    <dbReference type="STRING" id="683960.A0A1E3P815"/>
    <dbReference type="GeneID" id="30200551"/>
    <dbReference type="OrthoDB" id="372421at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071031">
        <property type="term" value="P:nuclear mRNA surveillance of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043628">
        <property type="term" value="P:regulatory ncRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09862">
        <property type="entry name" value="PIN_Rrp44-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000010">
        <property type="entry name" value="Exosome complex exonuclease DIS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.700:FF:000001">
        <property type="entry name" value="Exosome complex exonuclease exoribonuclease (Rrp44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041505">
        <property type="entry name" value="Dis3_CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022966">
        <property type="entry name" value="RNase_II/R_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033771">
        <property type="entry name" value="Rrp44_CSD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033770">
        <property type="entry name" value="RRP44_S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF35">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RRP44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17849">
        <property type="entry name" value="OB_Dis3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17216">
        <property type="entry name" value="Rrp44_CSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17215">
        <property type="entry name" value="Rrp44_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01175">
        <property type="entry name" value="RIBONUCLEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10" id="KW-0271">Exosome</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="PIN" evidence="16">
        <location>
            <begin position="84"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="RNB" evidence="17">
        <location>
            <begin position="511"/>
            <end position="844"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ61518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="980" mass="111586" checksum="6021245C7C806544" modified="2017-01-18" version="1">MTTPLVNKRKITDGLTVTSKVFVRSRNGNASKIVREHYLRNDIPCLSKKCNKCFQILTPNAQGELPKFILSEDPLNIKALGGAHYVIVDTNIILQAIDLLENNAIFFDVIIPQTVLDEVRNKSFPIYTRIRTLCKDNDKRFIVFHNEFNEETFTKRLDNETINDRNDRAIRNVAKWFQSHLDDKIKMVLITNDRLNKEAALKDKIITKTLHEYVDLYPNGENFKDLIPTEDSFKPREQSTTIYYKDYYPTSRLMGGLKNGTLYQGAINISSYNFLEGEITIPTLPKPLLILGNENLNRSFNGDSVIVELLPKSKWKKPSTMIIDDENLNSNDDPENDDVTELTDKERRLLTQDAISTQSNDQDELQPTGRVVGIVKRSWRLYVGQIVANSVSKSLTNSAKSCFVKLMDKSLPRIRIKTRRAQELLGKKIVIAIDSWNTTSKHPEGHFVKSLGESESVEAETEALLLEHDVEYRPFSKNVLDCLPEEGHDWIVPQDLQDEQVLAKDPLLPKRKDYRDKLICSIDPPGCVDIDDALHAKQLPNGNWEVGVHIADVTHFVKAGTALDAEGASRGTSVYLVDKRIDMLPMLLGTDLCSLKPYVERFAFSVIWELDQDANIVNVDFNKSIIKSREAFSYEQAQLRIDDQSNQDELTKGMRALLQLSKKLKQQRLDAGALNLASPEVKVHMDSETSDPGEVEIKKLLETNSLVEEFMLLANISVARKIFNEFSQTAMLRRHSPPPSTNFEKLNDMLRIRKNLSISIESSKALADSLDRCIDPNDDYFNTLVRIMSTRCMMAAEYFSSGSYGYPEFRHYGLAVDIYTHFTSPIRRYCDVVAHRQLAAAIGYEPLDLTHRDKHKMELICKNINKRHRNAQFAGRASIEYYVGQVMKNNESTETGYIIRVFSNGIVVLVPKFGVESLIKLEDLTIHVNDAKFDEAEYSLNFKTNDGDRRTVAVFDKVQVEVKSVLDQSVGKRKAQLILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8A9</accession>
    <name>A0A1E3P8A9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_77829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040389.1">
        <property type="nucleotide sequence ID" value="XM_019184622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8A9"/>
    <dbReference type="GeneID" id="30201868"/>
    <dbReference type="OrthoDB" id="10535469at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052413">
        <property type="entry name" value="SUR7_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019">
        <property type="entry name" value="CELL MEMBRANE PROTEIN YLR413W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019:SF2">
        <property type="entry name" value="CELL MEMBRANE PROTEIN YLR413W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="308" mass="34323" checksum="166AD27375326DCE" modified="2017-01-18" version="1">MWLIDRERKQPGSIWSLLTCLTTLILLCVALGGLSSRSNGIKSVFIAHLDFSNANWQMVVPGLDNFRGHGFPPIQRMNLWNNCNGDYDMNGGFDAINPYRTWYSTSLDPRYAVSHQLNSLNPGAFTRDEINTPSGTVIPRVQSGTIIVLTTIACVLNGIAFFCQLLRIPGFVIPKIIAVIFLLVANTMVHVLAKRMVNSFNNNYGTFGIQADLGGRYLAMVWNAFGILLISAALQFTRGPGGIMGNYYEDNSPKFDPEAMKHGYTPNYNSQQMNKQPNDMGYNNMNNYNNGQMVGNYPNEHPPPPAYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8E8</accession>
    <name>A0A1E3P8E8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_81897</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040855.1">
        <property type="nucleotide sequence ID" value="XM_019185307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8E8"/>
    <dbReference type="GeneID" id="30202553"/>
    <dbReference type="OrthoDB" id="3980979at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="294" mass="33243" checksum="C7D0344180306C1E" modified="2017-01-18" version="1">MTSVYYESYSEISTPANSDYLTEEESDEVETESENEHTSSSRSSSSSGLLTRSETNESIEVINTSRLLDSSVDTLAGLLQRSSLGPKVLNSIQSGEQTENNSGIATPQLFTLEEAKKILAAEKKNAEHASIIGRVNKVLRSNEGKISDDPRGFGFKFLCLCITAAFGGRRGIARYDRWESKYDFTSIIPSITDCSNHLRQLNSTMMCYIGHVILYYWDEEPFRRAMISKFGPLDKIIKGKDNIRSEIGAKNLWLPKVREVHSLTKKKIIEEFEGRFEKDTENFEKILLFFGIEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8J3</accession>
    <name>A0A1E3P8J3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_18745</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040936.1">
        <property type="nucleotide sequence ID" value="XM_019180805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8J3"/>
    <dbReference type="STRING" id="683960.A0A1E3P8J3"/>
    <dbReference type="GeneID" id="30198051"/>
    <dbReference type="OrthoDB" id="1738954at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="11"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="314" mass="35564" checksum="ABFF3CE3BA2A4A11" modified="2017-01-18" version="1" fragment="single">LPQYKELSPRYIIQEQIGEGAFSKVFKAFDIELNKHVAIKIIEKQAMNKSQIDSVLKEVSIMKRLNHPNIVTILNFINNDSKSFIILELLNGGEIFNKIVELTYFSELLSKHVIIQIVQAIKYLHQEVGVVHRDIKPENLLFDKIPYKPSTTPPQLRKSDESNKKDEGEFITNIGSGEIGVVKLADFGLSKILWDQATTKTPCGTASYTAPEIIKDEAYSKAVDMWGVGCVLYTLLCGFPPFYDNNPEELTKKVARGDYCFLSPWWDEISKEAKDLVSHLLTVDPKKRYTPDDVLNHPWITGVEKLITPAIDAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8M9</accession>
    <name>A0A1E3P8M9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Anaphase-promoting complex subunit 4 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_102477</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040781.1">
        <property type="nucleotide sequence ID" value="XM_019180355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8M9"/>
    <dbReference type="STRING" id="683960.A0A1E3P8M9"/>
    <dbReference type="GeneID" id="30197601"/>
    <dbReference type="OrthoDB" id="10262475at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990298">
        <property type="term" value="C:bub1-bub3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033597">
        <property type="term" value="C:mitotic checkpoint complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044774">
        <property type="term" value="P:mitotic DNA integrity checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990942">
        <property type="term" value="P:mitotic metaphase chromosome recapture"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990758">
        <property type="term" value="P:mitotic sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140499">
        <property type="term" value="P:negative regulation of mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902499">
        <property type="term" value="P:positive regulation of protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="251"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="350" mass="38673" checksum="7C7738E38F330E7D" modified="2017-01-18" version="1">MSGTGIVELPDSPKDIVSSIKFSPVYNHLLASSWDGIVHLYSTDPQSTQNDSLLKIDTHTPVTDITWDGFTWKKAFLSNVDGEVKEIDLENARATNVIGTKHDSGIQALTYIPNSRGILVSGSWDKSIQYLDCRLQNSGKLGSTAYHTKLPEKVFAMDATENNVVVAMSNRLVHIYDIRNPTSPLQIRESGLKYQTRSLKCMPNGSGYAQSSIEGRVAIEYFDPSSKVQAQKYAFKCHRLPCSDVDLVSPVNALSFHKKYGTLFTAGSDCHVCLWDQSSKKRLRQYSKFDQSVVCLDTDYKDGNSILAIATSDDSFKTVATLDGGAPLPSKSSIYLKYLGENEGMPKTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P934</accession>
    <name>A0A1E3P934_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_13873</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041118.1">
        <property type="nucleotide sequence ID" value="XM_019180587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P934"/>
    <dbReference type="STRING" id="683960.A0A1E3P934"/>
    <dbReference type="GeneID" id="30197833"/>
    <dbReference type="OrthoDB" id="5065855at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019784">
        <property type="term" value="F:deNEDDylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044613">
        <property type="entry name" value="Nep1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468:SF1">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="5">
        <location>
            <begin position="6"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="213" mass="24446" checksum="C315624FD2CF48F5" modified="2017-01-18" version="1" fragment="single">LQFFDISIYKEDLDNLKDDEWLNDNNISFIYEYLERYQLNNFDKLVSNSIILLRPSMVYLLANHPSPKELKGVLPSLETSKFIFLPVNDNDDVEDACAGSHWSLVVISLLDKTALIYDTLERANESEAKHVISQLAKYLGFNFQIRIIEKTPQQINGSDCGIMVGQITGFLLSRLLHLKYLEDHFIDMDLNNVEISAIDGRIFIMGTLLNLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P960</accession>
    <name>A0A1E3P960_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">VASt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_60020</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YSP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041153.1">
        <property type="nucleotide sequence ID" value="XM_019182921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P960"/>
    <dbReference type="STRING" id="683960.A0A1E3P960"/>
    <dbReference type="GeneID" id="30200167"/>
    <dbReference type="OrthoDB" id="2162691at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140268">
        <property type="term" value="C:endoplasmic reticulum-plasma membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13220">
        <property type="entry name" value="PH-GRAM_GRAMDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051482">
        <property type="entry name" value="Cholesterol_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319:SF4">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="755"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="8">
        <location>
            <begin position="539"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="205"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="451"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="47"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="84"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="252"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="897" mass="98678" checksum="B19904D31B8634BA" modified="2017-01-18" version="1">MDTMDDAWSYSYPSSLESLPLPPLSSTNGDKVDSEVAGGAQGNGKPNGKDREKLKIVTEQPKEIKLESPIALRRDSTGNDSTKAGSSPPKPSAMSAPQPALLPRHRNSTTTSQNNLAKSAPADPHTTKHDVESLPADNSSTSSINDTATDLAMIQNQSPPSTPVQGSNFFNNMISTLSFNKGKDGKPSTSMNSIDENGVVTVTMDSHSRTNSHGGSAKDSTTSPSMLSYRRDKRSKRRNTSASSIDIPKSPLVEDKSITEETSPKSETFDTSNYVDEKFKDTSYRYATITRNNDFHKLFKSIPADERLLDDFSCALSREILLQGRLYVTEKNICFNSNLLGWVTNLVIPYADISKFEKTATAGLFPNGIAINLNNGNKHYFASFLSRDSTYNLLTEVWEASDDQANNLSQVNTNSFQSTEGEALVSKDLNLGTEKYDADEYQKALLSIDGDTRTNRRNTSGSDEFYDDDDDDDDDDEEDDSDESSNSEYSGSGKVSEAGKVVSSSQQVVYKAKNTSVYAYTGPDGHQSTSSGIDYTAMQEVILAEENIKCPPGLLFEILFGPNNDLTLQFLGSQGGSNFSKFSDYERNDEGKKERNYNYTKALNYSIGPKSTKCYVQEAIVSLDYDGCTNVLSTTKTPDVPSGNAFSVKTRYVMTWGPENTTNLVISFKVDWTGKSWVKSMIDKSCLSGQEEATKQLIPLVKKIVDDNTFATEEEIKIENNIEPLADAKRADIDAKSLSSAKVTPSTPDSFIPEFSAFNIAVLALLSGILLLQVGILRSIRSVPEKTFSFGVLSEIDKSTENVLFKGEEVLLWNWLNERAGNLDLSQAEKTGVLKNDIDQVINKWVSNDLNSKESKKLIKTFEHHLNSYSEKALNKMKDEQHQQKAAALKAAIKALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9G1</accession>
    <name>A0A1E3P9G1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_76229</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041256.1">
        <property type="nucleotide sequence ID" value="XM_019184346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9G1"/>
    <dbReference type="STRING" id="683960.A0A1E3P9G1"/>
    <dbReference type="GeneID" id="30201592"/>
    <dbReference type="OrthoDB" id="1026733at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000837">
        <property type="term" value="C:Doa10p ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000839">
        <property type="term" value="C:Hrd1p ubiquitin ligase ERAD-L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034389">
        <property type="term" value="P:lipid droplet organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072671">
        <property type="term" value="P:mitochondria-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006577">
        <property type="entry name" value="UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF1">
        <property type="entry name" value="FAS-ASSOCIATED FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00594">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="3">
        <location>
            <begin position="414"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="339"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="529" mass="60894" checksum="435653C589FBC926" modified="2017-01-18" version="1">MPDHISPSEQEKLDQFQMITAFKDDEYDKVVALLRNNWWNLEVALSKYFDGDIDREAARSPPPVEATPFGASSSAFDTQTLGGQSSGFNNSEMTLNQFDSWSHIDAQNPLLSTHGLLPQLPIVRPLSNNRRFQPGLNNSTSSSLWNQFTHSPILFILLIIPRTVTLLLTAVEYLFSFIFPSSPDHLPDKPTLEKFDFKEHFESLIADRSETQFFEGDFNDAFNKAKNETKFLLTVLIGENESSNQFLKKVFNNNTICNLIKEEQVIVYAGNVNEAQSLALARNLKTIVVPAVYLIGNVASGPNAIASMSILSRIPVKSVNSFVTKLRREIEKYKPEFITRKIEQDELNYSRQIKELQDQAYEESLIADKLKQEKREAEKLEKLKKEQEADFKRENKNKFYASLLDKFDNLAHLPKGEFTNIRFKLPSGEHVMQKFSKEDTLFDVYSFVALKLHLKELSEQDLEDLKKISNDIHEDYKHEFEFELISPFPRYVVPVKEDQHVKEVNQLWPNGSVMIEYLVDEDDDSEVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9Q0</accession>
    <name>A0A1E3P9Q0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">WICANDRAFT_82252</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent rRNA helicase required for pre-ribosomal RNA processing. Involved in the maturation of the 35S-pre-rRNA and to its cleavage to mature 18S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with the SSU processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041352.1">
        <property type="nucleotide sequence ID" value="XM_019185359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9Q0"/>
    <dbReference type="STRING" id="683960.A0A1E3P9Q0"/>
    <dbReference type="GeneID" id="30202605"/>
    <dbReference type="OrthoDB" id="10261904at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17955">
        <property type="entry name" value="DEADc_DDX49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF24">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="19">
        <location>
            <begin position="2"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="33"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="242"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="400"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="14">
        <location>
            <begin position="2"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="400"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="431"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ62145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="441" mass="49001" checksum="14F33628E4DB062C" modified="2017-01-18" version="1">MSTFEDLGIDKWLAESLNAMKIHTPTAIQKACIPKILNGHDCIGGAKTGSGKTIAFGAPMISKWSADPMGIFGLILTPTRELALQIAEQFAALGANMNIRVAVIVGGEDMVKQAIKLQNKPHFIIATPGRLADHILNSGEDTVEGLRRVRYLVLDEADRLLSNSFGGDLERCFSILPPADKRQTLLFTATVTDAVRALKDKPRAENKPELFIHEVDTVDKVAIPSKLSTEYIFIPSYVKEAYLYGVLSHEDNEKSTAIVFVNRTHTAEVLRRTLRNLDIRVASLHSEMPQTERTNSLHRFRAGAARVLIATDVASRGLDIPSVQLVLNYDIPADPDDFVHRVGRTARAGRSGESISFVTEKDVTRILAIEERINKKMDKYEKISDNKVIKNTLQKTSVAKREALMDMEKEEFGEKRRANKRKATSDEPAKKSKKSKGKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9W2</accession>
    <name>A0A1E3P9W2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_26906</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041412.1">
        <property type="nucleotide sequence ID" value="XM_019181315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9W2"/>
    <dbReference type="STRING" id="683960.A0A1E3P9W2"/>
    <dbReference type="GeneID" id="30198561"/>
    <dbReference type="OrthoDB" id="10264728at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031416">
        <property type="term" value="C:NatB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000065">
        <property type="entry name" value="N-terminal acetyltransferase complex ARD1 subunit homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051646">
        <property type="entry name" value="NatB_acetyltransferase_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45910">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45910:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="188" mass="21994" checksum="B8ED082DC3511DB6" modified="2017-01-18" version="1">MSSLTPFQATDLFKLNAINLDTLTENFNIGFYLEYLTQWPSLFFKSEEFNDNISGYMMGKTEGSNELWHTHITAVTINHYYRRIGLASYLCDNLEYMTDAKPHETRFIDLFVKVDNQLAINLYEKLGYSVFRRVIGYYGGEHEYPQDTSVVNDLKDAFDMRKSMGRDLGESVRSDGREHRVLPHEIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9X1</accession>
    <name>A0A1E3P9X1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_76394</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome which plays a central role in DNA double strand break (DSB) repair. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041422.1">
        <property type="nucleotide sequence ID" value="XM_019184374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9X1"/>
    <dbReference type="STRING" id="683960.A0A1E3P9X1"/>
    <dbReference type="GeneID" id="30201620"/>
    <dbReference type="OrthoDB" id="3979296at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000008">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 13" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="12 13" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="15">
        <location>
            <begin position="8"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="16">
        <location>
            <begin position="93"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ62215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="132" mass="14044" checksum="BF3AFA8349B8C7D1" modified="2017-01-18" version="1">MSGGKGGKAGSSEKASQSRSAKAGLTFPVGRVHRLLRKGNYAQRVGSGAPVYLTAVLEYLAAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGHVTIAQGGVLPNIHQNLLPKKSTKGAKASQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9Z6</accession>
    <name>A0A1E3P9Z6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Aspartate-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="4">1.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_75909</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the NADPH-dependent formation of L-aspartate 4-semialdehyde (L-ASA) by the reductive dephosphorylation of 4-phospho-L-aspartate. Mediates the second step in the biosynthesis of amino acids that derive from aspartate (the aspartate family of amino acids), including methioinine and threonine, the latter of which is a precursor to isoleucine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-aspartate 4-semialdehyde + phosphate + NADP(+) = 4-phospho-L-aspartate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.2.1.11"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:24286"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.11" evidence="4"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040914.1">
        <property type="nucleotide sequence ID" value="XM_019184288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9Z6"/>
    <dbReference type="STRING" id="683960.A0A1E3P9Z6"/>
    <dbReference type="GeneID" id="30201534"/>
    <dbReference type="OrthoDB" id="1894490at2759"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00016"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00463"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00464"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009090">
        <property type="term" value="P:homoserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18130">
        <property type="entry name" value="ASADH_C_arch_fung_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02315">
        <property type="entry name" value="ScASADH_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000200">
        <property type="entry name" value="Aspartate-semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000016">
        <property type="entry name" value="Probable aspartate-semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051823">
        <property type="entry name" value="ASADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000319">
        <property type="entry name" value="Asp-semialdehyde_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005676">
        <property type="entry name" value="Asp_semi-ald_DH_pep-lack"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00978">
        <property type="entry name" value="asd_EA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006416">
        <property type="entry name" value="PRK08664.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718:SF1">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000148">
        <property type="entry name" value="ASA_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01103">
        <property type="entry name" value="ASD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0791">Threonine biosynthesis</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="337"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Semialdehyde dehydrogenase NAD-binding" evidence="15">
        <location>
            <begin position="6"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="13">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000148-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ61707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="365" mass="39125" checksum="123B4AFEF5077B90" modified="2017-01-18" version="1">MAPTKKAGVLGATGSVGQRFILLLSDHPDFKIEVLGASSRSAGKPYSEAVTWKQTSLLPESAKSIPVSECTVEAFKDCDVVFSGLDSDYAGPIEKAFVEAGLAVVSNAKNYRREPDVPLIVPIVNTDHLSIIESKLKKAEEAGEKRPGFIACISNCSTAGLVAPLKPLVDAFGPIDALTATTLQAISGAGFSPGVSGIDVLDNIVPYISGEEEKIEWETKKILAPLNKDQTGVELLSPEEIKVSAQCNRVAVSDGHTECISLRFKNRPAPSVEQVKQVLRDYVCDAYKLGCHSAPKQTIHVLEEADRPQPRLDRDRDAGYGVSVGRIREDPLLDFKMVVLSHNTIIGAAGAGILIAEILLSKKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA54</accession>
    <name>A0A1E3PA54_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_76473</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041502.1">
        <property type="nucleotide sequence ID" value="XM_019184390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA54"/>
    <dbReference type="GeneID" id="30201636"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="277" mass="30825" checksum="9D8BB8E365499773" modified="2017-01-18" version="1">MAQFIRRNNNNIDVISSTSRPVSSASLQECVETSSSSDLIVQTNNSPTTDNFQTVRIEDHEINPSLPSLIYDNYTLMQPKEKQQIINEPLTSPELNSQSFQSALDTYHQPLTTGDMTAIGDYELETQQVPDDELAGHVNTIHEQDSAQTITAPVTQNQINPMVTVTILSRLFKFVKYLIGLDPPTNQDPERRPLLSPDVENQQPPPTTPGQGPAYNLLSRYRYFFIVLTILIGIGLITVVLATGDSKLLVLVFKAFLCYLLGSNAQYLFGQDFCPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA67</accession>
    <name>A0A1E3PA67_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_60373</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the snRNP Sm proteins family. SmF/LSm6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041516.1">
        <property type="nucleotide sequence ID" value="XM_019182979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA67"/>
    <dbReference type="STRING" id="683960.A0A1E3PA67"/>
    <dbReference type="GeneID" id="30200225"/>
    <dbReference type="OrthoDB" id="268799at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005732">
        <property type="term" value="C:sno(s)RNA-containing ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01726">
        <property type="entry name" value="LSm6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000037">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF1">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006609">
        <property type="entry name" value="snRNP_SmF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10 13" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8 13" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Sm" evidence="14">
        <location>
            <begin position="11"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR006609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ62309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="80" mass="8839" checksum="D955B3A8861B1577" modified="2017-01-18" version="1">MSEVTTTTISAPSKFISDISGAKVIVKLYSGEEYHGTLDSIDGYMNITLEETQEIVSNKVTRKYGDVFIRGNNVLYISQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA69</accession>
    <name>A0A1E3PA69_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_59918</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041049.1">
        <property type="nucleotide sequence ID" value="XM_019182901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA69"/>
    <dbReference type="GeneID" id="30200147"/>
    <dbReference type="OrthoDB" id="4070694at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="89" mass="9781" checksum="3C82F99E8EA91826" modified="2017-01-18" version="1">MGLFDSVKNQVGEENFSKIQGALGGSGDKKSGEQTDYVKKAEGYIGEDRLKQIKDKVGEDNYKKGEDAIRKQFGGSSSEAEKKTEEMLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA75</accession>
    <name>A0A1E3PA75_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine/threonine-protein phosphatase 2A activator</fullName>
            <ecNumber evidence="9">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Phosphotyrosyl phosphatase activator</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_17612</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the PTPA-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="9"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041523.1">
        <property type="nucleotide sequence ID" value="XM_019180753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA75"/>
    <dbReference type="STRING" id="683960.A0A1E3PA75"/>
    <dbReference type="GeneID" id="30197999"/>
    <dbReference type="OrthoDB" id="16120at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008160">
        <property type="term" value="F:protein tyrosine phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04087">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004327">
        <property type="entry name" value="Phstyr_phstse_ac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043170">
        <property type="entry name" value="PTPA_C_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037218">
        <property type="entry name" value="PTPA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03095">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016325">
        <property type="entry name" value="Phstyr_phstse_ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140984">
        <property type="entry name" value="PTPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0697">Rotamase</keyword>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ62316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="407" mass="47202" checksum="83917CB2216FAC42" modified="2017-01-18" version="1" fragment="single">FDYSEPVKRIHDSQGLHLFQSSIALYELQKSLTTIVQLVENKEVPNGVLSKELLYTQQEIDQYSHDLHAPVPMFDQDSSTDSTFLQKVLKEKPYIKSLLDLFHEMSVLIDHVPPLEGPRRYGNLACREWHSKLEDQIDEMLERLLIGPYYKLPNPQGFLNELKWYIINAFGSKERLDYGTGHELNFLAFITGLWKTNVLNQDEIDGYDFLIIFGRYYDLVRKLITLYTLEPAGSHGVWGLDDHFHISYILGSSQFLNNSSSSASSAKHYPTVSPKMVNNKSVVYQYSTKNLYFNSIAFIYKVKKGAFFEHSPILYDISNVKSWEKILKGMLKMYSVEVVNKFPVVQHFYFGNVLFPWRSLKNNTPLPVHTSEENLSEKTNYSNDEWKDKISSSSHQYHQVPIVTAPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAC1</accession>
    <name>A0A1E3PAC1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GPI inositol-deacylase</fullName>
            <ecNumber evidence="10">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_15066</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in inositol deacylation of GPI-anchored proteins which plays important roles in the quality control and ER-associated degradation of GPI-anchored proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the GPI inositol-deacylase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="10"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041548.1">
        <property type="nucleotide sequence ID" value="XM_019180628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAC1"/>
    <dbReference type="STRING" id="683960.A0A1E3PAC1"/>
    <dbReference type="GeneID" id="30197874"/>
    <dbReference type="OrthoDB" id="348976at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050185">
        <property type="term" value="F:phosphatidylinositol deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006505">
        <property type="term" value="P:GPI anchor metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034368">
        <property type="term" value="P:protein-lipid complex remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012908">
        <property type="entry name" value="PGAP1-ab_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039529">
        <property type="entry name" value="PGAP1/BST1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056824">
        <property type="entry name" value="PGAP1_TMD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15495:SF7">
        <property type="entry name" value="GPI INOSITOL-DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15495">
        <property type="entry name" value="NEGATIVE REGULATOR OF VESICLE FORMATION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07819">
        <property type="entry name" value="PGAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25141">
        <property type="entry name" value="PGAP1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25140">
        <property type="entry name" value="PGAP1_TMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133763" description="GPI inositol-deacylase" evidence="11">
        <location>
            <begin position="24"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="647"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="689"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="744"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="817"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="863"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="898"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="928"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase PGAP1-like alpha/beta" evidence="12">
        <location>
            <begin position="76"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase transmembrane" evidence="13">
        <location>
            <begin position="651"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="964"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF25140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ62341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="964" mass="108596" checksum="B19A5A7088589577" modified="2017-01-18" version="1" fragment="single">VSSFGLLVFVAIGILSTQDHIGADSPQCRPIYMYPSYARVTGFDSSHTKFASKYNLFLYREQGKDKVPTENEENILDGIPVLFIPGNAGSYKQVRSIAAEASNIFHDHKHEEQFKGRNLDFFSAHFNEDFTAFHGRTMLDQAEYLNDAIRFILSLYSNSSNPPESVLLLGHSMGGVVSRVMLTLPNYVENSVNTIISLASPHAAAPATFDGDIMRVYGATDKFWRAGFYGDESSISSRRLKNVTLISITGGLLDTTLPADYTIMNGLVPDTNGFTMYTTGIPGVWTPIDHLAIVWCDQLRKAVASTMLSIVDPSSSSRTYPLAKRMELFRRGLLSGFEDYAAQDFAIASNSTEVIVKLDKKDVVTSTGYRTMLSSQKTSIIDLTNIDPNSVFYFLSSLKPSQTIKDNSKPYLMFCEDNLGLPSRKQTIDLSLGASKDDTLEYECVNIGSDVYPVPKSTKDISEASRSSFGGDQSPFYAAQIKSDLFKKYKYILYAGPSDADSSFFATWSIDRFERQSTQVNNSILSLLLGATVELPESYSLVQNIQFNSIISSLLSYKVKFDFEKIEDMPFEPFARQWVEDPFETKWQLGLQENKKISFHGIAPYTPFKSASNPLHFQIWAPFSECPPMVFIKIDIIESLKLWVLRYRLAVVSLPVSIIVVTLILQFLSFSKTGYFPSFQEGLISFSRLMVPICLFVSVLSPLACNPGIQKLLYYVDLIAVTRPGELKDVHVNPYFLGIQEKQLWILGPIFMSVALSLVFTINFILQLISIVILQFYTVLKSTFNLFAGLKSKETKVALGKTTNGEITEKRRLAGTILLLGFVMFYIPFQFAYVVCCIVQGIVCLKAVSTRDLNFLNFNKSLFVLMLWIVPVNVPVLVVFFHNMAVRWETPFSSHHNILAILPIILTVGKNVQGFMLPQPQFPLQKKVTVFLLSYFAFFSLVYGVRHLYWLHYLFNFFCAWIFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAT6</accession>
    <name>A0A1E3PAT6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Methylthioribose-1-phosphate isomerase</fullName>
            <shortName evidence="6">M1Pi</shortName>
            <shortName evidence="6">MTR-1-P isomerase</shortName>
            <ecNumber evidence="6">5.3.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">S-methyl-5-thioribose-1-phosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Translation initiation factor eIF-2B subunit alpha/beta/delta-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MRI1</name>
        <name evidence="7" type="ORF">WICANDRAFT_26557</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5-(methylsulfanyl)-alpha-D-ribose 1-phosphate = 5-(methylsulfanyl)-D-ribulose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19989"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="EC" id="5.3.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 1/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eIF-2B alpha/beta/delta subunits family. MtnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.23" evidence="6"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041708.1">
        <property type="nucleotide sequence ID" value="XM_019181278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAT6"/>
    <dbReference type="STRING" id="683960.A0A1E3PAT6"/>
    <dbReference type="GeneID" id="30198524"/>
    <dbReference type="OrthoDB" id="2461at2759"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00874"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.420:FF:000006">
        <property type="entry name" value="Methylthioribose-1-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="entry name" value="translation initiation factor eif-2b, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01678">
        <property type="entry name" value="Salvage_MtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005251">
        <property type="entry name" value="IF-M1Pi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475:SF1">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 6" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="288"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="419" mass="46120" checksum="5DEBA9D78DE423B7" modified="2017-01-18" version="1">MSLQAIKFDRETISLSILDQLLLPYATEYITINTIADAHDVIKKMQVRGAPAIAIVGTLSILVELNIFLGDKFNGQSFYDLKDFNNFKSQLIQRLEYLVTSRPTAVNLSNAVNEVIEIIKSTSASTIDELYPQILKFGCDLIDDDYTNNVKLGENGKDWILEHLAKENFEGDFSVLTICNTGSLATSGYGTALGVVRSLWKHAEQFNTTINEDEPATKKIKTTTAKFSRVYPLETRPYNQGARLTAYELVYEKIPSTLVTDSMVTYLISSLNKDPSKPPIKACVVGADRIVKNGDTANKIGTYQLALICKPFGIKFLVVAPKTTIDLKTQHGDDIVVEQRPANELQNVSGGVIDLTTNEVVRNEKGYAVSGKVGVAAPGIEVWNPSFDITPHDLIDGIVTEDGVVTKVNGEFDLEALFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAV2</accession>
    <name>A0A1E3PAV2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_76664</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041705.1">
        <property type="nucleotide sequence ID" value="XM_019184431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAV2"/>
    <dbReference type="STRING" id="683960.A0A1E3PAV2"/>
    <dbReference type="GeneID" id="30201677"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133765" description="Peptidase A1 domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="3">
        <location>
            <begin position="51"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="491" mass="53505" checksum="FEA162E9BD02D0AC" modified="2017-01-18" version="1">MNTFLAINICLAYIQYAHAALNFQFNHNNQSHLMKRDDFNSDFEAGSTAAPIIDLYFGSQKQKIPIILDTGSFTPFLQSTDVSKNKGFDYKSSSSYEDKHQDFSVQYADSTSYKGTWSEDDVSNTENGQKFKLNFGLVTNASTQGVSSDTNVFGLGFDYDKPTLIDDLKDNNVISRKTFSIYSSANDHSLGQITFGAIDTEKYTGDLVAVPLRSSGNGYVWWTYLTDLSAGCKSATGGNTYFISFDCGYTTGVTLPSAVHEKLMNNFDAFYESDSQNYVFDCDNATPLEVEVMGHKIEVPTSGFTKKVKNSDKCVLQNINVGSDDNTITFGWVFFPYIYGVFDAENKELLFGKPVTDTSDSHIEDLHDSIPGSTQASLYSSVSDAVTTNSISRTSVQTSSTSIELEGTGVSSQGSCGNKRLARRFSLAADNTPTLATHVYEAVSETASAGSNVTANSSTAVPVSQYEDSASTFGQTSFILMLFSGIVALLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAX8</accession>
    <name>A0A1E3PAX8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aldehyde dehydrogenase 5, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_25826</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Minor mitochondrial aldehyde dehydrogenase isoform. Plays a role in regulation or biosynthesis of electron transport chain components. Involved in the biosynthesis of acetate during anaerobic growth on glucose.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041770.1">
        <property type="nucleotide sequence ID" value="XM_019181195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAX8"/>
    <dbReference type="STRING" id="683960.A0A1E3PAX8"/>
    <dbReference type="GeneID" id="30198441"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004030">
        <property type="term" value="F:aldehyde dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019413">
        <property type="term" value="P:acetate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07091">
        <property type="entry name" value="ALDH_F1-2_Ald2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000001">
        <property type="entry name" value="Aldehyde dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000001">
        <property type="entry name" value="Mitochondrial aldehyde dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="7">
        <location>
            <begin position="31"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00053679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00069627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="503" mass="55018" checksum="22F2E127BA748364" modified="2017-01-18" version="1">MAETVTIKFPTGESYKQPTGIFINNKFQPARSGDTSEVVNPATEEVITKVAKGNKDDVNEAVDAAEKAFETWSQTAPYERSLKLLKLADLIEQNADTIAKIETLNNGKALIWAKSDVQLLIRCFRSTAGAYDKIEGRTIDTQDGYFTYTKKEPLGVCGQIIPWNFPILMWAWKIGPAVITGNTVVLKPASATPLSALFCSQLIADAGFPPGVINTLIGSGRDAGDTMLRHPKIRKTAFTGSTAIGREILISSGESNLKKISVELGGKSPNIVFNDVEDRIDEVVDALIMGIFYNNGEVCCAGSRIYIQSGIYDKVVKKLKEKTEAIKVGDPFEEGVFHGAQALKSQFDTVLEYIEKGKKEGAKLLTGGERVGNKGFFIQPTIFETSSEDYSIVKEEIFGPVATIGKFTTADEAIKKANDSEYGLASGVHTNDLHVALEVSNRLQAGTVWVNTYNDLQESTPFGGYKQSGFGREMGIEVFDNYTQVKAVRMKYHHKTALSEGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB84</accession>
    <name>A0A1E3PB84_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Succinate-CoA ligase subunit beta</fullName>
            <ecNumber evidence="11">6.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_87350</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.-" evidence="11"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041354.1">
        <property type="nucleotide sequence ID" value="XM_019186102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB84"/>
    <dbReference type="STRING" id="683960.A0A1E3PB84"/>
    <dbReference type="GeneID" id="30203348"/>
    <dbReference type="OrthoDB" id="1552at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042709">
        <property type="term" value="C:succinate-CoA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000001">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000004">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00558">
        <property type="entry name" value="Succ_CoA_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_ligase-like_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001913">
        <property type="entry name" value="PRK00696.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815:SF1">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP-FORMING] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="SUCCINYL-COA SYNTHETASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="ATP-grasp fold succinyl-CoA synthetase-type" evidence="13">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-citrate synthase/succinyl-CoA ligase C-terminal" evidence="12">
        <location>
            <begin position="255"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ62147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="380" mass="40583" checksum="6E93A0D09D6728C8" modified="2017-01-18" version="1" fragment="single">YGVGVPNGDAATTPEGAYEIAKGLNTNDLVIKAQALTGGRGKGHFDTGFQGGVQLVNSPEKVKELAKEFLHHKIITKQTGAAGKEVTAVYVVERAYARTEAYLAILLDRATQKPMIVASAQGGMDIEGVAAKDPSAIKTFPIDLEAGVTDAQAKDIASVLGFTKDAEPAAAETVKKLYKIFTEKDSTQIEINPLSETEDHKVLCMDAKFGFDDNASFRQKEVFSWRDLTQEDPDEVKAAESNLNFIKLDGNIGCLVNGAGLAMATMDVIKLYGGDPANFLDCGGGATPETIENAFKLILSDQKVSGIFVNIFGGIVRCDYVAEGLIAATKNLGVKVPIVVRLQGTNFEKASELIKESGLKIYAVNELDEAAETVVKLSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBJ4</accession>
    <name>A0A1E3PBJ4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RWD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_88528</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat WDR59 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041955.1">
        <property type="nucleotide sequence ID" value="XM_019186162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBJ4"/>
    <dbReference type="STRING" id="683960.A0A1E3PBJ4"/>
    <dbReference type="GeneID" id="30203408"/>
    <dbReference type="OrthoDB" id="311712at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049567">
        <property type="entry name" value="WDR59-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="108"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="195"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="RWD" evidence="5">
        <location>
            <begin position="427"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="622" mass="70012" checksum="4E4539EE8EB68C25" modified="2017-01-18" version="1" fragment="single">MANITSPYESVTFGNSLSLRVDGGVGAVSINPSGRDVVLAGRRGLYVVDLDDPFSPPRWLHHLTSWEVADVQWSPHASKPAWVISTSNQKAMVWNLARTANSAIELVLHGHSRAITDINFHPDHPELLATCSVDTSVLAWDTRTPKRPFYTVSDWRAGASQVKWNFKNPNILSSSHDHYFYIWDLRNNSKPLLKINAHERKINGVDFSRTKESEIISSSNDNTVKFWDLAKDTENPVSVINTGFPVWRARHLPFGDGCSIMPLRGGNNSIYLASHSEQEGDNKLKPCYVFKGHTDRVTDFLWRSRHSSSGVDDREFQLVTWSKDCDLRLWNMNDDIYDRVGFKRGEPKELLAYDYKTYREEPGTATNKGYALKKTKDTFVTSRGESINTDDHLTWISGVRIGRSAFAPPIDQNPNMTVGNAPDNLGEEVSVVGHKFPKVKFEKISVSTGTLVLSLNGPWGSNPEDLLFLRLETKYPRTYPSDPPTFRIEENRELVNEKRQEIMQNLNDIAKKFASVERFSLEACLRFLMGEKVDLSNLEKDDDDFNLPLEDFDDLSSLPSSISSSESEAEDDELIPLAQSGLNSAPTFDSTPIPKGCGASWTKSGQLVCFFIPQRESKSQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBN7</accession>
    <name>A0A1E3PBN7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Maf-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_25197</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042033.1">
        <property type="nucleotide sequence ID" value="XM_019181117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBN7"/>
    <dbReference type="STRING" id="683960.A0A1E3PBN7"/>
    <dbReference type="GeneID" id="30198363"/>
    <dbReference type="OrthoDB" id="10267058at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00555">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00528">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003697">
        <property type="entry name" value="Maf-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00172">
        <property type="entry name" value="maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213">
        <property type="entry name" value="BIFUNCTIONAL DTTP/UTP PYROPHOSPHATASE/METHYLTRANSFERASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213:SF5">
        <property type="entry name" value="BIFUNCTIONAL DTTP_UTP PYROPHOSPHATASE_METHYLTRANSFERASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02545">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006305">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="24142" checksum="FF7C273AD9EA4B6C" modified="2017-01-18" version="1">MSFQPFKHPVFDKIKEKRFILASTSPRRIEILTQMGFENVEVYPSNFPEDLKKEDYTPQDYVLNTAIGKAQAVYEELKNKGEAENTIILAADTVVEIDGQIFEKPKDKQDQLKNLTYYRDSKKVQHVLSGVVVINEGVVSSFVEDTALHFDYEASDEMLKAYVDTNEGLGVAAGYRVQLRGSLLMKKIDGDYYNAVGLPFRNTFKLIEKALGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PD39</accession>
    <name>A0A1E3PD39_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Isoleucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_25623</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="2"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042079.1">
        <property type="nucleotide sequence ID" value="XM_019181165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PD39"/>
    <dbReference type="STRING" id="683960.A0A1E3PD39"/>
    <dbReference type="GeneID" id="30198411"/>
    <dbReference type="OrthoDB" id="10264412at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07960">
        <property type="entry name" value="Anticodon_Ia_Ile_BEm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00818">
        <property type="entry name" value="IleRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02002">
        <property type="entry name" value="Ile_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033708">
        <property type="entry name" value="Anticodon_Ile_BEm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023585">
        <property type="entry name" value="Ile-tRNA-ligase_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050081">
        <property type="entry name" value="Ile-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42765:SF1">
        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42765">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="10">
        <location>
            <begin position="47"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="11">
        <location>
            <begin position="723"/>
            <end position="880"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="972" mass="111187" checksum="7C5411C6089A08D2" modified="2017-01-18" version="1">MPVFRAQRRLYSDLAHKYSKTLRLPQTEFPKRSNYEATIKDLVPRSSQDLYKLQLEKSPIKDDVFILHDGPPYANGDLHLGHALNKILKDFINRFNLMMGKQVYYKPGWDCHGLPIELKALETINKKLKKDASMTLSVKEIRDMARNHATKTVETQLESFKQFAILADFKDNYKTMNHQFEINQLKIFQKMLSRNLITRQKKPVFWGCETHTALAEGELEYNDKHRSTAAYLSFPLVEMTDELKHLLKDLESPSVLIWTSTPWTIASNRAISINENFTYTIIKSSEQSLIVAKELASQLLEIKPEFSETFIEIPGTMLVGSSYVNPISNETHPIIHGDHVIDNAGTGLVHTAPGHGNEDYFACLKHGMEVYSPVDNFGKYTKDVPSGFESLVGKRVLGEGGKIMLNLLEDAKMIFHVDPNYIHSYPYDWRSKKPIIIRATPQWFANVGKIKEAASKSLDDVKFTPEKGKNRLLSFIKNRNEWCISRQRSWGVPIPALYSKHDENIVLMDQESVSHIISKIDELGTDAWFEVEENVERWLPESHRGQGADFYKGKDTMDVWFDSGSSWNEIQSFIEKHDLKRDYLADVYLEGSDQHRGWFQSSLLTKIATDDASTTKAPYKNIITHGFTLDEKGQKMSKSIGNTILPENVIQGKNGLPALGVDGIRLWAAQADYTSDMAIGPKILKHVGDSLKKIRITFSFLLGNLNDIKPEEIVEYDNLRPIDKYALSKLVKLNEEILEQYRVFNYNRVIQLLNYHLNTELSATYFDIVKDRLYTDKVDGLSRRAVQSVLLEIHQTYISILSPVLPIVTQEVWDFTLPWIKENQPSPFLKGCKKLDSRYKNSELEAEFDSIWKIKDEVKLLAEQGRKNDKTIRNSLETDVVVNISGKDTGLENIMQKHAQYLADYLLVSHVHINKPLPDSLAYHYSNSVLIDGTTVTVSVVPSSESKCPRCWKYTSVSAEDLCQRCDEVLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU25</accession>
    <name>A0A1E3NU25_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_37092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454217">
        <property type="protein sequence ID" value="ODQ56685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035892.1">
        <property type="nucleotide sequence ID" value="XM_019182512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU25"/>
    <dbReference type="STRING" id="683960.A0A1E3NU25"/>
    <dbReference type="GeneID" id="30199758"/>
    <dbReference type="OrthoDB" id="5414461at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ56685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="49" mass="5349" checksum="CF1CAF684A32CF6A" modified="2017-01-18" version="1">MCEGKKSSLSSMDSDLEAFSHNPADGSFAAMPDQTAAKTNYPNERFLSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU52</accession>
    <name>A0A1E3NU52_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CN hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_37162</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454231">
        <property type="protein sequence ID" value="ODQ56646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035853.1">
        <property type="nucleotide sequence ID" value="XM_019182525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU52"/>
    <dbReference type="STRING" id="683960.A0A1E3NU52"/>
    <dbReference type="GeneID" id="30199771"/>
    <dbReference type="OrthoDB" id="10250282at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050345">
        <property type="entry name" value="Aliph_Amidase/BUP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674">
        <property type="entry name" value="NITRILASE C965.09-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674:SF12">
        <property type="entry name" value="NITRILASE C965.09-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="2">
        <location>
            <begin position="8"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ56646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="308" mass="34836" checksum="A276D56DF93E1815" modified="2017-01-18" version="1">MTKGIRKFTIAGVQLGPILKETKKERTVERLVSLLEKAAESNASLAVFPELALTSFFPRWFIESQENVDSYFEKAKKFKIGFVLGYAELTDQGNHFNTCILVNDKGEIVHKYRKCHLPGFDEPTEGIEFQQLEKRYFEYGDLGFPAYRSDPEWIDATIGMLICNDRRWPEAWRAYALQGMDLCLNGYNTAANGTSLFGSKENKELRQYHSDLSTTANAYFNSCFAVSVAKCGVEDGQGLIGGSIIVDPNGKVIAQAKTEDDELLIAEIDLEDTINGKQKMFDFSRHRRPELYSRLVDQKGSVETDYVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUC4</accession>
    <name>A0A1E3NUC4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_37096</name>
        <name evidence="2" type="ORF">WICANDRAFT_37099</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
            <strain evidence="2">NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454217">
        <property type="protein sequence ID" value="ODQ56683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="KV454217">
        <property type="protein sequence ID" value="ODQ56686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035890.1">
        <property type="nucleotide sequence ID" value="XM_019182515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUC4"/>
    <dbReference type="GeneID" id="30199761"/>
    <dbReference type="OrthoDB" id="4071950at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ56683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ56686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="49" mass="5770" checksum="1A3279533F313584" modified="2017-01-18" version="1">MPRHLISDAHEWINEIPTVPIYYLAKPQRRERASQNQRGKKTLLSLTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUK3</accession>
    <name>A0A1E3NUK3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial inner membrane protease subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_81599</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S26 family. IMP2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035970.1">
        <property type="nucleotide sequence ID" value="XM_019185265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUK3"/>
    <dbReference type="STRING" id="683960.A0A1E3NUK3"/>
    <dbReference type="GeneID" id="30202511"/>
    <dbReference type="OrthoDB" id="9996127at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042720">
        <property type="term" value="C:mitochondrial inner membrane peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.109.10:FF:000005">
        <property type="entry name" value="Mitochondrial inner membrane protease subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037730">
        <property type="entry name" value="IMP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46041">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46041:SF2">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Peptidase S26" evidence="12">
        <location>
            <begin position="21"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ56763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="176" mass="20189" checksum="FDEA5270BF5985FD" modified="2017-01-18" version="1">MINPVYKNGFKYGYRVSLLVASWIPVMIVLNEHVCYVGKIEGSSMRPTLNPTSKLQDWVLLWKWGVRDYNGLKVNDVILFRSPTNPEKVLCKRVKGVQGDTILTRYPYPREQCHIPRNHLWVEGDNLHSIDSNTFGPISTGLVIGTATRIIFPFTRWMEIPNGGREARISKIEAFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUU4</accession>
    <name>A0A1E3NUU4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">D-aminoacyl-tRNA deacylase</fullName>
            <ecNumber evidence="2 6">3.1.1.96</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_81732</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a D-aminoacyl-tRNA + H2O = a tRNA + a D-alpha-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>glycyl-tRNA(Ala) + H2O = tRNA(Ala) + glycine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the DTD family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="2 6"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036113.1">
        <property type="nucleotide sequence ID" value="XM_019185291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUU4"/>
    <dbReference type="STRING" id="683960.A0A1E3NUU4"/>
    <dbReference type="GeneID" id="30202537"/>
    <dbReference type="OrthoDB" id="275783at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097358">
        <property type="term" value="F:D-leucyl-tRNA(Leu) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051500">
        <property type="term" value="F:D-tyrosyl-tRNA(Tyr) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106026">
        <property type="term" value="F:Gly-tRNA(Ala) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900832">
        <property type="term" value="P:D-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900829">
        <property type="term" value="P:D-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00563">
        <property type="entry name" value="Dtyr_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.80.10:FF:000001">
        <property type="entry name" value="D-aminoacyl-tRNA deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="entry name" value="D-tyrosyl-tRNA(Tyr) deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00518">
        <property type="entry name" value="Deacylase_Dtd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003732">
        <property type="entry name" value="Daa-tRNA_deacyls_DTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00256">
        <property type="entry name" value="D-aminoacyl-tRNA deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10472:SF5">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10472">
        <property type="entry name" value="D-TYROSYL-TRNA TYR DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02580">
        <property type="entry name" value="Tyr_Deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69500">
        <property type="entry name" value="DTD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ56906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="150" mass="16329" checksum="72747B88D7765878" modified="2017-01-18" version="1">MKVVIQKVKSASVTVDTKVVSSIQKGLMLLVGISTEDTEQEVEKLSSKILKLRLFEDESGTMWKKPITEINGQILSVSQFTLMARTKKGSKPDFHLAAKGADAKVLYESFLSKLKANIGHENVQDGVFGAMMDVALVNDGPVTITIDSTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUW6</accession>
    <name>A0A1E3NUW6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_65254</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036198.1">
        <property type="nucleotide sequence ID" value="XM_019183880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUW6"/>
    <dbReference type="STRING" id="683960.A0A1E3NUW6"/>
    <dbReference type="GeneID" id="30201126"/>
    <dbReference type="OrthoDB" id="129353at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17745">
        <property type="entry name" value="BRCT_p53bp1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17724">
        <property type="entry name" value="BRCT_p53bp1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047249">
        <property type="entry name" value="BRCT_p53bp1-like_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047250">
        <property type="entry name" value="BRCT_p53bp1-like_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013914">
        <property type="entry name" value="Rad9_Rad53-bd_dom_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047252">
        <property type="entry name" value="TP53BP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15321:SF3">
        <property type="entry name" value="TP53-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15321">
        <property type="entry name" value="TUMOR SUPPRESSOR P53-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08605">
        <property type="entry name" value="Rad9_Rad53_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="5">
        <location>
            <begin position="366"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="95"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="128"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ56991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="635" mass="72144" checksum="25C022F9C91057D7" modified="2017-01-18" version="1" fragment="single">QGAPKQVTKSHLTSSPNVEESSPAIREKADTFEIEKAKADDNNNIETNTINTSNKSFHSQAVDFNALAENITQEFSDVDDESKVVDNTIEEEADNLKLQTEWNEGTSKSSNPVIRPTRNSKRKKSSKANDLQLQFTSSPDKSINHSDLSIVLDANTSQIDDNDHDISTNDQQSILTPEYDVNVLPNGILREETHNLSEEDIRFKDSVWCLANTRYYPGLILSLGKKGLEVKFFESTDEVNGGIFPLDIQIGDTVKIGRLVYLVTGLECVIQNLKEKAIRCMRGYDTVIVKRVKGKGKSMKILDTEEKFPLAEVHVENEEWHRRPRVLNEIKPKRSSINQVQQLKRIIPKTYEEELVLSTPKKPKKTTSDIFHECVFVITGEVDKDGLTKAIVMNGGTVLDDGFASMIDFSNGEPKSDAFKDFKFGALIAEKYSRSPKYLETVALGWPLLFEDFIYDCISDNKLLDNISTYLLASGESSKFRGEVKSADIFKFIQGYISKTDLSQQLRNNRILESKNVFICSKKNIEITQFLFKILGAAKVSFVPITNYTNLISTVTEHSYNKDILIYHDKISSLKSVLDHKVNDNKRRRIKKNSILSEVLSSNQKLQMELVDWEWLVQCVISRHPWKSQFKWSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV35</accession>
    <name>A0A1E3NV35_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_86002</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036268.1">
        <property type="nucleotide sequence ID" value="XM_019185981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV35"/>
    <dbReference type="STRING" id="683960.A0A1E3NV35"/>
    <dbReference type="GeneID" id="30203227"/>
    <dbReference type="OrthoDB" id="5977743at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000093">
        <property type="entry name" value="Cell division cycle-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033308">
        <property type="entry name" value="PGAP5/Cdc1/Ted1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315">
        <property type="entry name" value="METALLO PHOSPHOESTERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315:SF4">
        <property type="entry name" value="METALLOPHOSPHOESTERASE, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="429"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="6">
        <location>
            <begin position="66"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="454" mass="52373" checksum="4B082D96C79AA2FB" modified="2017-01-18" version="1">MVESAVLAGNSVQSKIRSKKSQSKRVVLVIWMALVNYYERSVVKRAMNVCQWTKWEKWNNGNDPHRIALFADPQIMDNYSYPGRPWIVNYFTQKVLDNYHKRNWNYVQNKLDPDSTIFLGDLFDGGRNWDDEDWYVEYTRFNSIYSKKANRKTIMTLPGNHDIGFGETVNVTSLERFKTFFGPTSSVHELGNHTIVLLDTISLSDYSNEAVTKDPRQVLESLADYKTDENPRILLTHVPLYRFPDSQKCGPLRESTKKFPIMKGLQYQTVIDHELSQDILSKVRPKLVFSGDDHDYCHITHPYKYQGQDKTAEEITVKSCSMNMGIKYPAIQLLSLNNPTSSQQQDEDETYKTNICYMPQPFRAIKAYISWAIINTILFLVVFLAPYHWAIITNKINAIVSSQSNKYSLPIAEVTQTSYTPTLTQERDVLGFFINGGIVIVSVLMIFAIYFNAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVN3</accession>
    <name>A0A1E3NVN3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Multiprotein-bridging factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_97936</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transcriptional coactivator that stimulates GCN4-dependent transcriptional activity by bridging the DNA-binding region of GCN4 and TBP (SPT15), thereby recruiting TBP to GCN4-bound promoters. Involved in induction of the ribosome quality control (RQC) pathway; a pathway that degrades nascent peptide chains during problematic translation. Required to prevent stalled ribosomes from frameshifting.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MBF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036476.1">
        <property type="nucleotide sequence ID" value="XM_019186618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVN3"/>
    <dbReference type="STRING" id="683960.A0A1E3NVN3"/>
    <dbReference type="GeneID" id="30203864"/>
    <dbReference type="OrthoDB" id="10253401at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140469">
        <property type="term" value="P:GCN2-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990145">
        <property type="term" value="P:maintenance of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000015">
        <property type="entry name" value="Endothelial differentiation-related factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013729">
        <property type="entry name" value="MBF1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10245:SF15">
        <property type="entry name" value="ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10245">
        <property type="entry name" value="ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1 MULTIPROTEIN BRIDGING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08523">
        <property type="entry name" value="MBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="7">
        <location>
            <begin position="85"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="150" mass="16287" checksum="72BCDDA52856EECD" modified="2017-01-18" version="1">MSDWDNVTIIGSKARVGGGGPRTNVARTQGQINAARRTGQVLSVDKKYSTGNQKGDVEGQRLTKVDRETDVVAPKKIDATVGKTISKHRTEKKLSQKDLATKINEKPNVINDYEAGRAVPNQQVLGKLERALGVKLRGKNIGDPLFGPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW09</accession>
    <name>A0A1E3NW09_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Complex I-B22</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">NADH-ubiquinone oxidoreductase B22 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_81519</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Mammalian complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I LYR family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036511.1">
        <property type="nucleotide sequence ID" value="XM_019185247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW09"/>
    <dbReference type="STRING" id="683960.A0A1E3NW09"/>
    <dbReference type="GeneID" id="30202493"/>
    <dbReference type="OrthoDB" id="13598at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20263">
        <property type="entry name" value="Complex1_LYR_NDUFB9_LYRM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045292">
        <property type="entry name" value="Complex1_LYR_NDUFB9_LYRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033034">
        <property type="entry name" value="NDUFB9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12868:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12868">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B22 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="17">
        <location>
            <begin position="15"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="74"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ57304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="106" mass="12566" checksum="E46E98422439C4A3" modified="2017-01-18" version="1">MSQVTPFSPANRKVVQSLYHRSLKLAFNWINRRDLYRQKAVEIRAQFDLHKDVSDPKELRRLIDKTESLLEKYKHPDPFIPPQRAGGTKFERNTPPCLDEPYPARY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW90</accession>
    <name>A0A1E3NW90_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Ribosome assembly factor mrt4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_80730</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Component of the ribosome assembly machinery. Nuclear paralog of the ribosomal protein P0, it binds pre-60S subunits at an early stage of assembly in the nucleolus, and is replaced by P0 in cytoplasmic pre-60S subunits and mature 80S ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Associates with the pre-60S ribosomal particle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
            <location evidence="7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036592.1">
        <property type="nucleotide sequence ID" value="XM_019185112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW90"/>
    <dbReference type="STRING" id="683960.A0A1E3NW90"/>
    <dbReference type="GeneID" id="30202358"/>
    <dbReference type="OrthoDB" id="10262308at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05796">
        <property type="entry name" value="Ribosomal_P0_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1730:FF:000005">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000003">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033867">
        <property type="entry name" value="Mrt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051742">
        <property type="entry name" value="Ribosome_Assembly_uL10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841:SF1">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 MRTO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="8">
        <location>
            <begin position="132"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="238" mass="27357" checksum="736268CF01074773" modified="2017-01-18" version="1">MPRSKRSKLVTLAQTDKKGKQNKERIFDEIREALDQYRYVWLFYLDDVRTPVLQDIRSDWIGSKLILGKRRVLEKALGDTKEEEYKENLHKFSKLANGVSGLLFTNEDVETVASYFTAYSKQDYCRAKSKSPIDFTIPEGVIYSRGGQIPEDEDVAMSHSLEETFRNKFKIPTKIKAGKITISEPYHVVSKGEVLDVRQALILKQFGIAASEFKVKAKAYYDNESSEIKIVNINIDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW97</accession>
    <name>A0A1E3NW97_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Extradiol ring-cleavage dioxygenase class III enzyme subunit B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_85671</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DODA-type extradiol aromatic ring-opening dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036686.1">
        <property type="nucleotide sequence ID" value="XM_019185927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW97"/>
    <dbReference type="STRING" id="683960.A0A1E3NW97"/>
    <dbReference type="GeneID" id="30203173"/>
    <dbReference type="OrthoDB" id="7396853at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07363">
        <property type="entry name" value="45_DOPA_Dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014436">
        <property type="entry name" value="Extradiol_dOase_DODA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004183">
        <property type="entry name" value="Xdiol_dOase_suB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30096">
        <property type="entry name" value="4,5-DOPA DIOXYGENASE EXTRADIOL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30096:SF0">
        <property type="entry name" value="4,5-DOPA DIOXYGENASE EXTRADIOL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02900">
        <property type="entry name" value="LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53213">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="Extradiol ring-cleavage dioxygenase class III enzyme subunit B" evidence="7">
        <location>
            <begin position="68"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="371" mass="40878" checksum="9ADB6FAC0ECBBE65" modified="2017-01-18" version="1">MASRSFWSVGISLFIIIIALFGSRLQFFTQRLFGVTIARSRANMTTSATAFAASTATAATASSTKTPVFFFSHGGPTFMYPGSGFGGDQGAYNKVQSLGKYIQQTIKPKFIIVVSAHWQSNSNDRVEIGVPSSYSKNVFSSSKDLLDPQELDLIYDFYGFPKHMYDEKFHAKGNVELAQDIKETLSSQNIDSKLTSRGLDHGVWVPFKVAFSSNNPKDKEWDLDCPLVQVSLSASDDFEFHHRLGQALSKYRSLGGIVICSGMTVHNLRDLGIAMDRGGKPLAYVSKFNDLLSSIIASSKGEERLTKLENVQENERSLLYHAHPTLEHFMPVVVAAGAASAEAGQELYNNASLSLGWGVYQFGEYNTQEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWD5</accession>
    <name>A0A1E3NWD5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_64792</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036653.1">
        <property type="nucleotide sequence ID" value="XM_019183805.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A1E3NWD5"/>
    <dbReference type="STRING" id="683960.A0A1E3NWD5"/>
    <dbReference type="GeneID" id="30201051"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005285">
        <property type="entry name" value="Drug-R_PDR/CDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00956">
        <property type="entry name" value="3a01205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="510"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="542"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="583"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="620"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="652"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="760"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1176"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1211"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1252"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1299"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1332"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1452"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="144"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="842"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ57446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1511" mass="170722" checksum="FE7E4A6DD1CE2D5E" modified="2017-01-18" version="1">MSDHNKENNLHNNSQQNTLNDYNGFDSNVDQSIHELARRLTNASQKYPELGVDGDDVLSTTSSIAPGVVTVNNSDVDPRLDPSSDDFNSRYWIKNFKRVMDKDPDHFKHYSLGIVYKNLRALGDATDADYQTTVLNAPLKYVQKSLKSVFSSKESKHTQQFDILKPMDANVKPGEVVVVLGRPGSGCSTLLKTIAANTHGFQVGEESQISYEGVKPQDIKKHYRGEVIYNAETDIHFPHLTVWQTLSLAAKFRTPQNRIPGISREDYANHLTEVYMATYGLSHTRNTKVGNEVVRGVSGGERKRVSIAEVSLSGARLQCWDNATRGLDAATALEFIRALRTQADILDTTAFVAIYQCSQDAYDLFDKVSLLYEGYQIYFGRADEAKGYFVKMGYHCPARQTTADFLTSITSPRERVASKGYEQRVPKTPKEFEQYWKESPEYAQLIKDIDSTLIKQKQENSQDVIKSSHNQKQSKHLRPGSSYTVSFWMQVRYLLIRDFQRILNDLGFNLFQVFANSLIALVLSSIFYNMQKTTGSFFYRGGAMFFALVFNGFQSFIEIMTLFEARPIIEKHKQYSLYHPSASALSSIISQLPSKMIISVAFNLIFYFMVNFRRDPGRFFFYYLMNITATFSMSHLFRLVGSASSTLPASLVPAHFLLLALMIFTGFVIPVNYMLGWSRWINYLNPLAYAFEALMVNEFSGREFECSSFIPGDPRTTPGIPSNGFVCDSVGAEVGSFTVSGSTYLETAYKYRASHKWRNWAINLGFTLFFLAVYMIFSEYNESAMQKGEVLLFQRSTLKKLKKQHGDIKNDLEAGSEKQITEQDEEEGEQNVDVIHAGTDIFHWRDVHYSVKIKKETREILNGVDGWVKPGTLTALMGASGAGKTTLLDVLANRVTMGVVTGNMFVNGHLRDNSFQRSTGYVQQQDLHLKTATVREALQFSADLRQPKDVSKAEKDAYVEEVIKILDMEKYADAVVGVAGEGLNVEQRKRLTIGVELAAKPKLLLFLDEPTSGLDSQTAWSICQLMRKLANHGQAILCTIHQPSAILMQEFDRLLFLAKGGQTVYFGDLGKNCQSLINYFENHGAQKCPPDANPAEWMLHVIGAAPGSHANQDYHQVWLQSSERQEVLQELDRLETELVKLPRDTSVGQEEYAAPLWKQYVIVTKRIFQQHWRSPIYIWSKMVLAVVSSLFIGFSFFKAKNTRQGLQNQMFGFFMYLMIFNPLLEQGLPTFVEQRGLYETRERPSKTFSWKAFIAAQITAEIPWNALVGTAAFFCFYYPVGFYNNASPSGTAAVNQRGAYAWFFNVLFYIYIGTTSHLVMAPLELTESAGNVGYLIFTLGLTFCGVLVSLNKLPGFWKFMYRVSPFTYIVDGFLSNGLANNRVQCSSTELVRFSPPSGYNCAQYMESYITKAGTGYLADPLATENCEFCAISSTNDFLRVVGVSYDRRWRNVGIFIAFIIFNIVGATFLYWLARVPKKADRVKCVKKEGEVNVPQSEYSKSTIEKEDSTIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWT3</accession>
    <name>A0A1E3NWT3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">KOW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_64941</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036800.1">
        <property type="nucleotide sequence ID" value="XM_019183836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWT3"/>
    <dbReference type="STRING" id="683960.A0A1E3NWT3"/>
    <dbReference type="GeneID" id="30201082"/>
    <dbReference type="OrthoDB" id="1539250at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.70:FF:000001">
        <property type="entry name" value="60S ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3260:FF:000001">
        <property type="entry name" value="60S ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.70">
        <property type="entry name" value="Ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001147">
        <property type="entry name" value="Ribosomal_eL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018259">
        <property type="entry name" value="Ribosomal_eL21_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036948">
        <property type="entry name" value="Ribosomal_eL21_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20981">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01157">
        <property type="entry name" value="Ribosomal_L21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01171">
        <property type="entry name" value="RIBOSOMAL_L21E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="18452" checksum="0AE3C51D5D13B23D" modified="2017-01-18" version="1">MTNTHGYRRGTRYMFQRDFKKHGVIALSTYMKTYKKGDIVDIKGNGAVQKGMPHKWYHGKTGVVFDVTRSSVGVIIYKVVGNRYIEKRVHLRVEHVQHSDSRKEFLVRVKENAQKKKEAKEKGEAVQLKRQPGLPRGARVVSTQGNKPITIRPLPYETYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWZ9</accession>
    <name>A0A1E3NWZ9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_70748</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036873.1">
        <property type="nucleotide sequence ID" value="XM_019184214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWZ9"/>
    <dbReference type="GeneID" id="30201460"/>
    <dbReference type="OrthoDB" id="4017072at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="666" mass="76890" checksum="FDF8FBFA2AD72376" modified="2017-01-18" version="1">MLEQVPYCDYSILKRKKNLFSEELHPYLRKVTTVGDYIRNVNDFHHSKKVRTFTRSKKNKFEAIFSILLHTLHYACANTSAVPKSFLKLSHNLPQEVEYILKSTKSSQFLKQAHELDPSFQKEVAQLVNLYSYGDTKLSFDTRKIDQKLLKNIEISYAALLLQLDNRHEIQHLLQWISSIMAPTVTKCCQSLTGLSTFPNVVIKDVLLREPTSEYEFDLMFDLFQHYAEVNTVFSEVLIKNMIHFANRYKFTKLEELVQIILTKGGQSDDAMLNRLVCSLADLGEMKSSRNHYLSVLNAQRLIIDKILERKGEIAPLGYLGIARGLIPLSPQRAGKFLLFVLDQKLTTEESQLASIIKLKLSQSPDTLIRTFDSTMGDVTLEKSPDLWKELFINLEHFNLLNEQNSLGFLKKVETPQFSDAKNELLSHLDLNDILEKTITMNNRSLGIILDKLYRSKKHFHVFGEGYNYARHIFHNISSPSRSTVGVVLLGESFENPSHAYDLYLTLLEKHCDNIPNEKCLLSLLIPPYLDPTLKWGNRYASQIAVHEFRKHCKRSLDDVEGTVIPSNSLWKPYVKLLGRCNYQDELSEVMKLWEDLNVIPERETLAMLLASLPMGVGESKRSHAIKYLVPNEDSGQIQDLSETPNWDWPDDHELYFQREKIVQRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXH3</accession>
    <name>A0A1E3NXH3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_57603</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036579.1">
        <property type="nucleotide sequence ID" value="XM_019182808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXH3"/>
    <dbReference type="STRING" id="683960.A0A1E3NXH3"/>
    <dbReference type="GeneID" id="30200054"/>
    <dbReference type="OrthoDB" id="432719at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056236">
        <property type="entry name" value="HMA_PCA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01511">
        <property type="entry name" value="ATPase-IB1_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46594">
        <property type="entry name" value="P-TYPE CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46594:SF4">
        <property type="entry name" value="P-TYPE CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24534">
        <property type="entry name" value="HMA_PCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00943">
        <property type="entry name" value="CUATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="426"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="731"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="760"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="8">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="788" mass="85507" checksum="630F5E04419C741D" modified="2017-01-18" version="1">MTCTGCSKKVLKVLQNIEGISSINVTFVTGIAEFTINKDILDVPQAVARISKETGFKLTKIEYDFQTIDMIIGEGDLNKLEEFVESIEKLDKSTYRVSYDPQFIGARSLVSKIPGSKLAPPKFNKALEEGKKKLRLMWIKCALSLALAIPVVVLDWSHNSVPYATKSIVSLVLASLVQIIAIPEFYIGALKALIYSKVLEMDMLIVISITAAYVFSLVIFILKRVGVSISINEFFETSSLLIALVLVGRLLSEIAKVKAVSSISLRSLQIETSILLQDGDEIEIDSRLLEMNDKIVISPHSRVVTDGFIVSGEGPIDESMITGECLPVMKKVNDEVIAGTFNGSSKLIAQVTRLPGKNSITDIAESVENALAEKPKVQDLADKIAGYFIPVVIMISTIVFIVWIIVGLKVRHQSGGKAVSVAITYGIAVLAISCPCAIGLAVPMVLIIAGGVAAREGIIIKQSNVLENAYKVTDVVFDKTGTLTDNEMRVEDFKILEDNGIENDEVISVVKQMTLNNTHPVSVAVANFLDQKDASSAAKIEKIESISGSGIQCEFKHSILKLGNPYWTKLHNHEVVKPLLEKGYTLLCCASDSKLICVFSLRTELKPEAKDVIQFLSKKGQTCHIVSGDNRKSVEMVANELGISLSNIQFRATPKSKQAFIKSLKGASKEKVVLFCGDGTNDAIAVAQADIGVQIGTASEVTKASSDVILLGGLDGIVKLQSLSKRTFNRVFFNFIWSAIYNLFAILLSSGAFVKARIPPAYSGIGEIVSIAPVVLAAISLSIKWKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXJ1</accession>
    <name>A0A1E3NXJ1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sphingolipid delta(4)-desaturase</fullName>
            <ecNumber evidence="8">1.14.19.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_85071</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Delta(4)-fatty-acid desaturase which introduces a double bond at the 4-position in the long-chain base (LCB) of ceramides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>an N-acylsphinganine + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = an N-acylsphing-4-enine + 2 Fe(III)-[cytochrome b5] + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:46544"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:31488"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="EC" id="1.14.19.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the fatty acid desaturase type 1 family. DEGS subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.17" evidence="8"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037083.1">
        <property type="nucleotide sequence ID" value="XM_019185832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXJ1"/>
    <dbReference type="STRING" id="683960.A0A1E3NXJ1"/>
    <dbReference type="GeneID" id="30203078"/>
    <dbReference type="OrthoDB" id="200948at2759"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042284">
        <property type="term" value="F:sphingolipid delta-4 desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03508">
        <property type="entry name" value="Delta4-sphingolipid-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011388">
        <property type="entry name" value="DES1/DES2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013866">
        <property type="entry name" value="Sphingolipid_d4-desaturase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879">
        <property type="entry name" value="SPHINGOLIPID DELTA 4 DESATURASE/C-4 HYDROXYLASE PROTEIN DES2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879:SF8">
        <property type="entry name" value="SPHINGOLIPID DELTA(4)-DESATURASE DES1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08557">
        <property type="entry name" value="Lipid_DES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017228">
        <property type="entry name" value="Sphnglp_dlt4_des"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01269">
        <property type="entry name" value="Lipid_DES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="88"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Sphingolipid delta4-desaturase N-terminal" evidence="11">
        <location>
            <begin position="26"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ57876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="356" mass="41778" checksum="031667F843842D5C" modified="2017-01-18" version="1">MSTITHRKNPSQPLTIQTPPSDAPISKLNDFYWTEETEPHTIRRKLILQKYPHVTKLCGPEPLTKFIVFGVVTLQLSLAYYLRDTDFLSWKFFLISYIIGATANQNCFLAIHELSHNLGFKKPLYNKLYSIFTNLPIGIPYSASFQPYHQLHHKYLGDEILDTDIPTNYEAIILSNVLGKSFFATFQILFYALRPMFITQIKFTYIHLVNILVQFSVDYYMVTHWGWKSLSYLIFSSFLAGSLHPCSGHFIAEHYIMNPPKSYDRTKDHPPVETYSYYGILNLFTWNVGLHNEHHDFPYVAWSKLFKLNEIANEFYVDLPKHESWTMVIVNFISDPNVLLYNRVKRENKRKVQPRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXV3</accession>
    <name>A0A1E3NXV3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_64056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037111.1">
        <property type="nucleotide sequence ID" value="XM_019183664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXV3"/>
    <dbReference type="GeneID" id="30200910"/>
    <dbReference type="OrthoDB" id="4058540at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="217" mass="24439" checksum="9CD8BE032EDB1DB2" modified="2017-01-18" version="1">MPAISPVPKRTPSAELLQHDGSDKSSDYLNLAEPPAASKTPDPSNLFPLKRRSSSNYQTALLGDQLKNVNFNQLNTTHGKKPQVNTDPLTTTSSHSSIENQYNPKGLDEESAINNEEEYYPTTGDMSPEGSKRYFQRRSTGNGADESRNSFNVQYRGSVSYPPQSTTKETAPNSEKYGVPRSYHSNQQSPSHYEEHKNEYFPTSFEYADFKRRMSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXY1</accession>
    <name>A0A1E3NXY1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">U2 small nuclear ribonucleoprotein A'</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_45418</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the U2 small nuclear ribonucleoprotein A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037258.1">
        <property type="nucleotide sequence ID" value="XM_019182587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXY1"/>
    <dbReference type="STRING" id="683960.A0A1E3NXY1"/>
    <dbReference type="GeneID" id="30199833"/>
    <dbReference type="OrthoDB" id="433501at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000026">
        <property type="entry name" value="U2 small nuclear ribonucleoprotein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044640">
        <property type="entry name" value="RU2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003603">
        <property type="entry name" value="U2A'_phosphoprotein32A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552:SF6">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00446">
        <property type="entry name" value="LRRcap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="U2A'/phosphoprotein 32 family A C-terminal" evidence="9">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="232" mass="26809" checksum="379EC899DBA39F64" modified="2017-01-18" version="1">MRLTPNVLLDAPSFINPQHERTLSLRSLKIPMIENFTVTKDVNEAIDLTDNDIKILGNFPILTRLKTLLLAKNRIQTIQDDFYTTVPMLESLSLISNSLASLSSLEPLKNCKRLKNLYILNNHISQQEHYRLFIIWLIPQLKVLDFMKIKDKERTQANELFGEVEEPTELAKTILNSKPKHILDNDKDDDQIKDVLKKLSEEDREKLKQQLKTATSLSEIDKIETALKNGYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY13</accession>
    <name>A0A1E3NY13_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_64243</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NUF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037306.1">
        <property type="nucleotide sequence ID" value="XM_019183693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY13"/>
    <dbReference type="STRING" id="683960.A0A1E3NY13"/>
    <dbReference type="GeneID" id="30200939"/>
    <dbReference type="OrthoDB" id="8194677at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051383">
        <property type="term" value="P:kinetochore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045132">
        <property type="term" value="P:meiotic chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.60">
        <property type="entry name" value="Ncd80 complex, Nuf2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005549">
        <property type="entry name" value="Kinetochore_Nuf2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041112">
        <property type="entry name" value="Nuf2_DHR10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038275">
        <property type="entry name" value="Nuf2_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN NUF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650">
        <property type="entry name" value="MEMBRALIN/KINETOCHORE PROTEIN NUF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03800">
        <property type="entry name" value="Nuf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18595">
        <property type="entry name" value="Nuf2_DHR10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="11" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Nuf2 N-terminal" evidence="13">
        <location>
            <begin position="18"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Nuf2 DHR10-like" evidence="14">
        <location>
            <begin position="267"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="161"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="308"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF18595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ58099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="451" mass="54047" checksum="7DD686D03467743D" modified="2017-01-18" version="1">MSRQSSIRRQNHEPVKSDRFPLLDIPELSLCLKSCNLSAPEEDLFKPTPIFVQSIFQQILHIFLSISPNRIRHRQQKLVEDEDEREEFEESLNLLSLQTILYKFFVDCGIYDFMVTDLTKPEPQRVKRLLSAVVNFARFREEHLYDNEAIMQENNLKFDKFQTNLDENKTLKKNIQGLEDRNNKINNNLDQLNEHNHKVEQELRNLKKIQESLTTQHLNYKTEKSRLIQSLEDHNYLLLESKKDLEKMKNYIIESPDIISKIIQDMNNSLESDQQALSDLELRSRKLAITIESFNLIQQDLKNCLKLVEELTIEINKENLNNNKLNQYKEKFEDNNLKVNELNRRIQLLTRQIKNNEEKTLRTTQQKDEKVKEYELKMQELHEIYATLMTEKNLNDRDMQKKKTYINSIQKKMYDLEMSFKKEYDETTLEIQRLNSHFKVYLNQIEEKLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY85</accession>
    <name>A0A1E3NY85_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19 20">CDP-diacylglycerol--inositol 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="5 20">2.7.8.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">WICANDRAFT_34180</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 20">
            <text>a CDP-1,2-diacyl-sn-glycerol + myo-inositol = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11580"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20 21">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.11" evidence="5 20"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037157.1">
        <property type="nucleotide sequence ID" value="XM_019182161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY85"/>
    <dbReference type="STRING" id="683960.A0A1E3NY85"/>
    <dbReference type="GeneID" id="30199407"/>
    <dbReference type="OrthoDB" id="10251079at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003881">
        <property type="term" value="F:CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000021">
        <property type="entry name" value="CDP-diacylglycerol--inositol 3-phosphatidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014387">
        <property type="entry name" value="CDP_diag_ino_3_P_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362:SF4">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--INOSITOL 3-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000848">
        <property type="entry name" value="CDP_diag_ino_3_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 20" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13 20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 20 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15 20" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="17 20" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00070582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRNR" id="PIRNR000848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="ODQ57950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="223" mass="25452" checksum="C1698494C8D5FAE2" modified="2017-01-18" version="1">MSSNSQSSTAIIFHYIPNYIGYLRVITAVISFQLMKSHPIYCTSIYAFSCLLDALDGHAARYYNQTSRFGAVLDMVTDRCTTSSLICFLCVLYPDYALIFQLLVSLDLASHYMHMYATLQTGGKSHKNIDKESSFLLNLYYTNRKVLFSICAFNELFYIALYLYYFPLYNITISGVPYSAAGVLAAICFPFYLFKQLTNIIQLKRASVMLAQLDVEDLQEKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY94</accession>
    <name>A0A1E3NY94_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_95594</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037386.1">
        <property type="nucleotide sequence ID" value="XM_019186500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY94"/>
    <dbReference type="GeneID" id="30203746"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="262" mass="30446" checksum="362BF588E9C2F9FF" modified="2017-01-18" version="1">MLSSRVPFALKALPLLNCRQNNLRLIKNHVFNQTRGLKFKVKRPEKPNVKPERPSKKKNTTKKEPTNDKNKANASQRKMMDAILKSSPKIEKEVKPRNTLEQSAPKTVMRPPKPNKDYISFADRLTPAVIQTSLGVTDEVFSKLIRRINFDGPCDSVILKLIYERVFYLELIEQYLASFPKGTDLNKVLQEKILTQNLKQSRDFIYTLRELKVEKYPEYFYTSSREVMSLKDLKSAKNITWMDRLKSHGMKALGMKSVFNHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYF5</accession>
    <name>A0A1E3NYF5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PITH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_34496</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PITHD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037446.1">
        <property type="nucleotide sequence ID" value="XM_019182191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYF5"/>
    <dbReference type="GeneID" id="30199437"/>
    <dbReference type="OrthoDB" id="2635at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.470">
        <property type="entry name" value="PITH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045099">
        <property type="entry name" value="PITH1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010400">
        <property type="entry name" value="PITH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037047">
        <property type="entry name" value="PITH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175">
        <property type="entry name" value="AD039 HT014 THIOREDOXIN FAMILY TRP26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175:SF1">
        <property type="entry name" value="PITH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06201">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51532">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PITH" evidence="3">
        <location>
            <begin position="27"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="238" mass="26959" checksum="477C81360ECB7F85" modified="2017-01-18" version="1">MSHSCEHEHHHGGHGHDGHDDHDHSHVAPIPTNESQSLRQYVDVFKLTGLNIAQPNDKIQKVFKDGDNKYQVSDYIESDADAQFILNIPFTGNVKLYSIILRTNGEAANCPKTIKLFKNKTNLDFDNINDTKPTYKIEQPQVGHLGDENGDDVDESSFVEHYLPRNLFQSTTQLSLFVENNWEDDEDELTKVYYMEIRGMFTSPLSKDPVITLYESAANPADHKNLLANEIGNQAGFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYL3</accession>
    <name>A0A1E3NYL3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_81121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036999.1">
        <property type="nucleotide sequence ID" value="XM_019185178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYL3"/>
    <dbReference type="GeneID" id="30202424"/>
    <dbReference type="OrthoDB" id="2103474at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025187">
        <property type="entry name" value="DUF4112"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35519">
        <property type="entry name" value="MEMBRANE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35519:SF1">
        <property type="entry name" value="YALI0C06193P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13430">
        <property type="entry name" value="DUF4112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="32881" checksum="9EBFC97FCE46FABF" modified="2017-01-18" version="1">MDAINNFAQKLEGVPGYDMAVDWINDKAGEKYQTKDPFYVEIHGKKKRRKAPETCSKQEQKSWKRVKKRAWLDDKNFFGCYPIDLGLGLAPLLTLVPVIGPLLMFAIHGRLVSIADQEFHLPPATIAKMHGNIGFDLLISLPPIIGSLFSWMNGCSTRNAAMVHTFLVKRERAKEEERLHQMNVNTDAPDNLSGAHSFQAYQNQRINSNQTSQGTQYKGRSSHQENITQPQQAFTRPQHHQVPPGSPAIRQEIPTVLPHSAQQSRKPPPPETYAQNTSNSTDYAQERGRQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYN9</accession>
    <name>A0A1E3NYN9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GTP-binding nuclear protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_34374</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the small GTPase superfamily. Ran family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037435.1">
        <property type="nucleotide sequence ID" value="XM_019182178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYN9"/>
    <dbReference type="STRING" id="683960.A0A1E3NYN9"/>
    <dbReference type="GeneID" id="30199424"/>
    <dbReference type="OrthoDB" id="48625at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00877">
        <property type="entry name" value="Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000131">
        <property type="entry name" value="GTP-binding nuclear protein Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002041">
        <property type="entry name" value="Ran_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071:SF0">
        <property type="entry name" value="GTP-BINDING NUCLEAR PROTEIN RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071">
        <property type="entry name" value="RAN GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00627">
        <property type="entry name" value="GTPRANTC4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51418">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="214" mass="24339" checksum="9B82CBDE96038542" modified="2017-01-18" version="1">MAQEVPTFKLVLVGDGGTGKTTFVKRHLTGEFEKKYIATLGVEVHPLAFFTNYGEIKFDVWDTAGQEKFGGLRDGYYINGQCGIIMFDVTSRITYKNVPNWHRDLVRVCENIPIVLCGNKVDVKERKVKAKTITFHRKKNLQYYDISAKSNYNFEKPFLWLARKLVGNPQLEFVASPALAPPEVQVDANLMQQYQQEMDQATALPLPDEDDADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYU2</accession>
    <name>A0A1E3NYU2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_22451</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037492.1">
        <property type="nucleotide sequence ID" value="XM_019180945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYU2"/>
    <dbReference type="STRING" id="683960.A0A1E3NYU2"/>
    <dbReference type="GeneID" id="30198191"/>
    <dbReference type="OrthoDB" id="10018191at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001732">
        <property type="entry name" value="Zgc:162936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000072">
        <property type="entry name" value="zinc finger protein 143 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF32">
        <property type="entry name" value="ZINC FINGER PROTEIN RST2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="3"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="55" mass="6663" checksum="24B7B5C4E656CD0C" modified="2017-01-18" version="1" fragment="single">RTFQCTGYPDCNMTFTRSEHLARHIRKHTGERPFECVHCSRKFSRLDNLRQHKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYY9</accession>
    <name>A0A1E3NYY9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Patatin-like phospholipase domain-containing protein</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_85437</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Lipid hydrolase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the PLPL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037596.1">
        <property type="nucleotide sequence ID" value="XM_019185891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYY9"/>
    <dbReference type="STRING" id="683960.A0A1E3NYY9"/>
    <dbReference type="GeneID" id="30203137"/>
    <dbReference type="OrthoDB" id="15478at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07232">
        <property type="entry name" value="Pat_PLPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021771">
        <property type="entry name" value="Triacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF66">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE PTL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11815">
        <property type="entry name" value="DUF3336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4 5 6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="8">
        <location>
            <begin position="237"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="633"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="5">
        <location>
            <begin position="268"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="633"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="672"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="690" mass="79749" checksum="71635B51AA36F339" modified="2017-01-18" version="1">MNINYIDEDREYFQSEITSKNDWTPIYTKLSSKKSSKKKKKHSTHKKTDDFFQSYSYRILRYPILGFILLWCSFLVIVYFFIRLYIALYEYFFTWTGQRRRLRLALRKSKDYAEWVSNAIKLDKFLKLDKWKTHPNFYYYDYKTLKITVKKLKALRTENSKEELALFLQGCIKANFAGTENPILYSQTYYGTKEIVEEYNTEVVKALEYIIEDDQISPLLKKDLFKSLNKNFGKTALCLSGGACFAYNHFGVIKALLESDLLPKIVSGTSGGGIVAALATTRTNEELEEVLVPELAQKITACNEPFSVYVKRWWKTGARFDAVDWARKAAWMTRGSMTFKEAYERTGKILNISTVPADPHSPVILCNHITSPNCVIWSAVLASSAVPGILNPVVLMMKDPKTKKVVPFSFGTKYKDGSLRTDIPVEALNTYFNVKFSVVSQVNPHISLFFFAPKGSVGRPVSRKKTGLRGGFIGAGLENFIKLENKKWLKFIKSLDLLPHLMDQDWSNVWLQRFSGTVTLWPKIKLKDFYFILSDPTEERLAEMIRNGELSTYPKLHFIKHRLNIERTIEKGLKKYKKQVRQLQKSQLGQEDAAKVEKLFSSESDIDDAGNTHGNYDLHSGINGLNYQVRFEQDEDDDEGSYNEEEDPDYVEEDEEEDEEDEDGEFDTDASQNKDDSFEGLRNRRKSTFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZD2</accession>
    <name>A0A1E3NZD2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_33524</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037730.1">
        <property type="nucleotide sequence ID" value="XM_019182082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZD2"/>
    <dbReference type="STRING" id="683960.A0A1E3NZD2"/>
    <dbReference type="GeneID" id="30199328"/>
    <dbReference type="OrthoDB" id="5376590at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.100:FF:000005">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 64 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF50">
        <property type="entry name" value="NADH DEHYDROGENASE (UBIQUINONE)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="7">
        <location>
            <begin position="174"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="External alternative NADH-ubiquinone oxidoreductase-like C-terminal" evidence="8">
        <location>
            <begin position="646"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="710" mass="79585" checksum="EAD7541E4EC64DFB" modified="2017-01-18" version="1">MLVPKKQLSHLIAVSNGLLKKNTNTFIKSIHTQANSQIYKNASIKPLFTSNGLLKTSKSSRFFSSATHLKYSELEFPKTIPKSKTFLNDTRFGKLVKYSIIAVGLGTTTIGTLILGFFIYDATTYHPDEIPDNIKINTLALNPNKGGPENLPVLKYNLDDYDTPEKEHLRYRPKLVVLGSGWGSVAVLKNLSHSDYDVTVISPTNYFLFTPLLPSAATGTLEVKTLIESIRKIVNNLDGHYLEAFADKVEFSDKLVKVHQFDKLTGEKQEFYVPYDKLVVAVGSNSNTHGVEGLEYCNQLKTAEDAVSIKKKITYLLEKACLPTTSDEERKKLLSFVVCGGGPTGVEFAAEIYDLLNEDLPKSYPNILRQELSVHVIQSRSNILNTYDERISEYATERFRKETIDVLTNSRVVKVLPDEVIFNQVNETTGEKELKSLPFGLCLWSTGVAQNPLAKEIVSTLHNSQRNKRAIETDAHLRVYGAPLGDVYAIGDCSTVRTDLADHTVEYVRKYIVDKHLKHISSNEIITDEDIKHLKLNYSEIFELGEEISKKHPQASEALNFLNELVPRYDVHQTGHLYFNQIADLLKDIDSRITSLPATAQRAHQQGKYLGKKLSKLAKSSASLTVNDILDGDIDDAVSKPFQYKHLGSLAYIGNSAVFDLPGYSFVGGLVAMYLWRGTYFAQSVSLRVRVLLFLDWLKRGLFGRDILSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZG1</accession>
    <name>A0A1E3NZG1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_64136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TTI2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037194.1">
        <property type="nucleotide sequence ID" value="XM_019183673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZG1"/>
    <dbReference type="STRING" id="683960.A0A1E3NZG1"/>
    <dbReference type="GeneID" id="30200919"/>
    <dbReference type="OrthoDB" id="6417021at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110078">
        <property type="term" value="C:TTT Hsp90 cochaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018870">
        <property type="entry name" value="Tti2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32226">
        <property type="entry name" value="TELO2-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32226:SF2">
        <property type="entry name" value="TELO2-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10521">
        <property type="entry name" value="Tti2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="486" mass="56030" checksum="85AEC6D39F9A1EB1" modified="2017-01-18" version="1">MNWENEVLDLLAGRSLDSGIPSAINLEIQEKYSDVKLDYLLSHTNQRAALAVLLAKIPILDTTEVIRTLENVLHLLKLPTIEWIWWVDDYTVTKGEYRIQIDFKEHARNLLIIAKEVVTQRKDLLNTYKMEILESVGAFNIDAPWCGESMLKISQEVLSHIDIDDEVLIQLVNGLKSKLSNLKNNKVSQAGYKKLNLNNGLRPRLGFNEIEDQRYKWKTENIDSISLFKLLLENLEGSKVKEYWWIISPTILNILDDYEGPIKLKGVELLSILLSKVDDDYLKLTGMIDIFYDAIAPLLTYLPKLTPTKQSVMIQKQTYPVLIRMFSKTPDFKDRLIKLLNTGIYQSINNVRDNFEILPILTDHMIRIVECLGITTVKCLPRLLYTLGMIIADPFIYLHQTLFSKALHCLIAILLNGWPRIENHKYDILGMIVLGFKKYEKSEDVEEKLKVLVALLRKVCDEDKLENDIKELVTNDSTLKGLFNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZJ3</accession>
    <name>A0A1E3NZJ3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptide chain release factor 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_32151</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037858.1">
        <property type="nucleotide sequence ID" value="XM_019181922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZJ3"/>
    <dbReference type="STRING" id="683960.A0A1E3NZJ3"/>
    <dbReference type="GeneID" id="30199168"/>
    <dbReference type="OrthoDB" id="2019491at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070126">
        <property type="term" value="P:mitochondrial translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000004">
        <property type="entry name" value="Peptide chain release factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050057">
        <property type="entry name" value="Prokaryotic/Mito_RF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804:SF7">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="Release factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="6">
        <location>
            <begin position="266"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="319"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="319"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00067174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="401" mass="45723" checksum="CFC29FFC9D1A71E6" modified="2017-01-18" version="1">MLRFHLGRSFRITQRPFGGAFSKRFNSTAPPQAFQQLHPSLVKRAEVLTQELKDLESSLADSGHFDVNQQKKYSRLSAIVDVFREYKENLTLYEELLPILKDESLKEDAEEEIKVTIPKLMDLSDKLKSRLLPPHEFAEKPCLLELRPGVGGTEAMIFTLELFNMYINYANHHRWPYTIVAKTNNGSGNGILEGIISIDEPGAYDKLRFESGVHRVQRIPETETKGRLQTSTAGVVVLPQMAEKNQSEDADERMFAPGEVRIDIMRASGKGGQHVNTTDSAVRLTHIPTGITVSMQDERSQHKNKTKAFNILRARLAEKERQEREAQERSMRTDQVSTTDRSDKIRTYNFPQNRITDHRCSYSLHDVEAVMKGEKLDLLIDELEKHEGQVKSKELENSTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZK3</accession>
    <name>A0A1E3NZK3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Rhomboid-type serine protease</fullName>
            <ecNumber evidence="10">3.4.21.105</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_21696</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Serine protease involved in intramembrane proteolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="10"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037800.1">
        <property type="nucleotide sequence ID" value="XM_019180913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZK3"/>
    <dbReference type="STRING" id="683960.A0A1E3NZK3"/>
    <dbReference type="GeneID" id="30198159"/>
    <dbReference type="OrthoDB" id="2146116at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002610">
        <property type="entry name" value="Peptidase_S54_rhomboid-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936:SF69">
        <property type="entry name" value="RHOMBOID-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936">
        <property type="entry name" value="RHOMBOID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="12">
        <location>
            <begin position="137"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ58593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="378" mass="42064" checksum="830C03949114B3E2" modified="2017-01-18" version="1" fragment="single">SSTNDLLLQHNERHHHHHRHPKYHIKKPSNTLGYKLLNFPYFTIIITIIDIAIFILELVKMNQLTGSAFQTKPYINPMLGPSSNVQIYLGSRFAPCIHPISGIDSGNLDWPCPNSTTLETQTCTISQLCNSDSNTPRQTWRLLSAMFLHAGFVHIIFNLLLQCTMGLDVERQIGSIRYIIIYIISGVSGNLLGLNFAQDGMSSTGASGALFGIIAVNLLIFILHRDKKTVRHYGILIGVLLFEVVACFVLGLLPGLDNFCHIGGFVSGLLLGVVLLNDPKFVRLDKNYSIDYGYKIQGFGNSISNHMDSIRRRKFAIWVIVRIVALTLLVAWFVGLSLNFTKNGGGHCSWCKYFNCLPVHNWCSQGDLASSISSNTSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZQ5</accession>
    <name>A0A1E3NZQ5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_64606</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037677.1">
        <property type="nucleotide sequence ID" value="XM_019183772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZQ5"/>
    <dbReference type="GeneID" id="30201018"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133580" evidence="2">
        <location>
            <begin position="19"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="167" mass="17610" checksum="333D8E553CD6AD93" modified="2017-01-18" version="1">MNIQLFFATFFIIRVALAASLSISSWMTRTIVVTNYGATYTKTPVELYTGQPTTTPTSGTYTTTIYVSNSMETYTKTMLKTYGLVSESTASTHLTVKNPSETYTKTLSTTLEVSSGGSKSESSTSATDAEALTSGSTSSSADGATGLQAVGRLAGFEALCAIAMMLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZT7</accession>
    <name>A0A1E3NZT7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Protein-S-isoprenylcysteine O-methyltransferase</fullName>
            <ecNumber evidence="3 10">2.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_100695</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine + S-adenosyl-L-methionine = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:21672"/>
            <dbReference type="Rhea" id="RHEA-COMP:12125"/>
            <dbReference type="Rhea" id="RHEA-COMP:12126"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90510"/>
            <dbReference type="ChEBI" id="CHEBI:90511"/>
            <dbReference type="EC" id="2.1.1.100"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.100" evidence="3 10"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037880.1">
        <property type="nucleotide sequence ID" value="XM_019180303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZT7"/>
    <dbReference type="STRING" id="683960.A0A1E3NZT7"/>
    <dbReference type="GeneID" id="30197549"/>
    <dbReference type="OrthoDB" id="422086at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004671">
        <property type="term" value="F:protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007323">
        <property type="term" value="P:peptide pheromone maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007269">
        <property type="entry name" value="ICMT_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025770">
        <property type="entry name" value="PPMT_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714">
        <property type="entry name" value="PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714:SF9">
        <property type="entry name" value="PROTEIN-S-ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04140">
        <property type="entry name" value="ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51564">
        <property type="entry name" value="SAM_ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="195"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="257" mass="29461" checksum="64EFB96DB5BE7E43" modified="2017-01-18" version="1">MNRFGKVYISEGCNVTFESDEGGPTPSTLDNDLLPDIKKNPPEEIALTAANLGILFGFAMATLFFSTYKEPSIYLMSLSVFHFLEFYITAKYNPKKVHKESFIINNGSSYTVAHTIAIIEAAVEYVFFPNFKKSFSWIKIIGLILLVGGQIIRSWAMITAGKSFSHLIVIKKSNEHELVTHGIYSVFRHPSYTGFFWWAVGTQLVLVNPIALVGFIIILWMFFSKRIKFEEKYLISFFGERYENYRKTAFVYIPFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P014</accession>
    <name>A0A1E3P014_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_93382</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038008.1">
        <property type="nucleotide sequence ID" value="XM_019186414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P014"/>
    <dbReference type="STRING" id="683960.A0A1E3P014"/>
    <dbReference type="GeneID" id="30203660"/>
    <dbReference type="OrthoDB" id="271595at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106335">
        <property type="term" value="F:tRNA (5-carboxymethyluridine(34)-5-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006448">
        <property type="term" value="P:regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051422">
        <property type="entry name" value="AlkB_tRNA_MeTrf/Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069:SF21">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="3">
        <location>
            <begin position="56"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="169" mass="18990" checksum="29092FF12B9A25D1" modified="2017-01-18" version="1" fragment="single">MCILFTTKSHHTSHKLDIKYVQMKLDFQQLHVTNLLKPWPIVEKFLTERIPGSIGIDVGCGNGKYLGVNKNVVMIGSDRSDGLISCAKDLGHEVMVSDGLSLPHPDNRFDFAISIAVIHHFSTPERRTQAIKHILSKLKKGGEALIYVWALEQENSRRGYKEGDPQDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P077</accession>
    <name>A0A1E3P077_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleolar protein 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 6">Pumilio domain-containing protein NOP9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_32165</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP9 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037962.1">
        <property type="nucleotide sequence ID" value="XM_019181924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P077"/>
    <dbReference type="STRING" id="683960.A0A1E3P077"/>
    <dbReference type="GeneID" id="30199170"/>
    <dbReference type="OrthoDB" id="392571at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040000">
        <property type="entry name" value="NOP9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22493">
        <property type="entry name" value="PUF_NOP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="534"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="616"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="637"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="648"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="658" mass="76655" checksum="9C8CB454D3371231" modified="2017-01-18" version="1">MGKVRGRRAQKEQKLKEAQAEHESEVKIYDESPSVSENKIDQEAVKEPNTFFGLVDTTELDYFKQAEATLNANAFESEADKAGFVASVLEESKGKELKLVTNQICSKLVERLILNCTDDQLKQLFKAFNGHFLSLIYHKYSSHCVETLLVRTAALIEKELLNPTSNYTNDDEYVSMENLFLFFLNEIKPNLKDMIIHQYASHTLRLIILIIAGKELPSTTVSNSTLRSKKSKIARKMIEIKDNQDFDKAFQIPSSFKDELKNLLDSIRSKLDQRTAREYAIHKVASPVLQLLIQVEGIVDRDRQFWHLVFLKDDEEKNSSEESFVEYLLSDPVGSHFFENTIKDQRLKYVERLYKLYMSERILKLAKRDTTGSYVVQILLKKLKQNEVRQILDQLIPEMSILMNSNLEMGKSIIDASTLHKDYKKPVIIEELVKKYTPNEESDILETVLQLSTSTLGNTRDDWPTAEERRRALFLEKLMEYDSSFLNLTVDNLLSLPKERLIQMCLHGVFSHVVEDVLKPEDVEIIKRRRLLNQFVGSIVELSCNAYGSHIVDKLWDFTLKLNNYKERIAQELIGEKEKVKESTYGRQVWKNWSMELFVRKRHDWTFKVKEQEYEKFPPKKPELPAAKSDNQFNGKRKSEGQEEPFQKKQKVRGRNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0D2</accession>
    <name>A0A1E3P0D2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_79361</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038022.1">
        <property type="nucleotide sequence ID" value="XM_019184877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0D2"/>
    <dbReference type="GeneID" id="30202123"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="171" mass="19308" checksum="E63A8F27A1CAA553" modified="2017-01-18" version="1">MNKSEFDPNNTSEELQLELSNSPANVRDASINLSTGMEKGSGSGFMLRMVHIKDIDKEESHHQEMNNNPSHRLNADDVPCEQLLGSSEAQTVSSEVFPESAEVEDSVSSAHESGHAHEINDAHHIDSGLTKTTIFERIFYYYQLLVYIFLLCCISYGFWNFIFDIAQDLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0G4</accession>
    <name>A0A1E3P0G4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_31757</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038080.1">
        <property type="nucleotide sequence ID" value="XM_019181877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0G4"/>
    <dbReference type="STRING" id="683960.A0A1E3P0G4"/>
    <dbReference type="GeneID" id="30199123"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11651">
        <property type="entry name" value="YPK1_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000048">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="253"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="509"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ58873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="579" mass="66524" checksum="D1B64F0DBFED0597" modified="2017-01-18" version="1">MTPETANQHLKVEKQDHLTHSSNSSSHTINSSNQSQQQQQAEIPDNNNFFKPGLLTLKIYNGSNFKIPFDIKNDKSILTRLINGDEHLINRLNQLNVNDDGDETIHPSNSSDLLPAIINIPPSILKNPLIYLTLEFDKTIIPIEPENGTISHPFFNKVSNFDVTKIQNELKIQVFLKIPKILSNINDEDILIGSKKINLKIDNNIKNPIRFKNHERLTFDNEYQQDDVKTNGDLIISIDFKPNENKHLTIDDFDLLKVIGKGSFGKVMQVRKKDSGKIYALKAIRKSHIVSKSEVTHTLAERTVLAKIDNPFIVPLKFSFQSPEKLYFVLNFINGGELFYHLQKEGKFELSRARFYTCELLSALECLHSFDIIYRDLKPENILLDYKGHIALCDFGLCKLNMKNEDKTSTFCGTPEYLAPELLLGQGYTKTVDWWTLGTLLYEMLTGLPPYYDQDTSTMYRKILTSPLKFPDNFDKDCKDLLIHLLNRDPTKRLGCNGSQEIKNHAFFKQIDWKKLYDKNYLPPFKPPVKDNIDTSNFDKEFTDEKPVDSVLNDYLSDSVQRQFGGWTYVGTEQLSGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0G5</accession>
    <name>A0A1E3P0G5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_17597</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037967.1">
        <property type="nucleotide sequence ID" value="XM_019180751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0G5"/>
    <dbReference type="STRING" id="683960.A0A1E3P0G5"/>
    <dbReference type="GeneID" id="30197997"/>
    <dbReference type="OrthoDB" id="4951845at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070692">
        <property type="term" value="C:CTDK-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032786">
        <property type="term" value="P:positive regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045903">
        <property type="term" value="P:positive regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20546">
        <property type="entry name" value="CYCLIN_SpCG1C_ScCTK2-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="2">
        <location>
            <begin position="38"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="2">
        <location>
            <begin position="145"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="287" mass="33766" checksum="8B3C35C08ADC7463" modified="2017-01-18" version="1" fragment="single">STIQLARPFLTKSQIKHAQERTIESSTVYEQRRNQIFNFLMKICVSLKFPTKTLETAMIYYQRYFLFNKFDIAIYYDIAITSIFIASKNEDTIKKLRDILLVSNQIRNTFLNQDQLELYRKKILSLEFKLLETISFDFRLFHVEDFLIKISKSLKIPKDIGYLAWLIAYDSYQTEIALKVPPQTIAIACISLATKLKDFETTIRSEFYYTSDNSVNEALLDLLGLYINSYNYTGLASIYPDFQNKFVEIRIPLSDAQGLKEQTADLIKEDDYFKDRDFSIGERRYML</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0L4</accession>
    <name>A0A1E3P0L4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNL-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_21735</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037679.1">
        <property type="nucleotide sequence ID" value="XM_019180917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0L4"/>
    <dbReference type="STRING" id="683960.A0A1E3P0L4"/>
    <dbReference type="GeneID" id="30198163"/>
    <dbReference type="OrthoDB" id="512667at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024158">
        <property type="entry name" value="Mt_import_TIM15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007853">
        <property type="entry name" value="Znf_DNL-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20922">
        <property type="entry name" value="DNL-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20922:SF13">
        <property type="entry name" value="DNL-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05180">
        <property type="entry name" value="zf-DNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51501">
        <property type="entry name" value="ZF_DNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNL-type" evidence="5">
        <location>
            <begin position="3"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="99" mass="11001" checksum="0C7A2DA014DBE13E" modified="2017-01-18" version="1" fragment="single">LKVDKPEMMIAFTCKKCDTRSSHVMSKQAYTSGTVLITCPGCKNRHLIADHLKIFSDDRITIQDILNAKGESVSSNTDDLVFDDIPEKLKGLIGHHAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0N7</accession>
    <name>A0A1E3P0N7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_84574</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038037.1">
        <property type="nucleotide sequence ID" value="XM_019185735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0N7"/>
    <dbReference type="GeneID" id="30202981"/>
    <dbReference type="OrthoDB" id="2213137at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF315">
        <property type="entry name" value="TRANSPORTER MCH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="229"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="462"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="496"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="530"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="99"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="51"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ58830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="621" mass="69726" checksum="1E089104F7C5B63B" modified="2017-01-18" version="1">MPNSKPTSQNDYQMQDIDIDNENTIRNEDDGDDTQSTLSSAVSHDAEFNPVQLNESQQSADLNNQTHPKLRSDTRDQTELNQELTRQLSVADAIFLNESRTERRGSSQTGEDASLDRLEEEQYEAEEQPLDRATSNVERIFTNKSTGQLDLPPDGGYGWVCVACVTAIQACTWGANAGYGIFLEYYLSNNVFTNATAYDYALIAGLIVFTAQFCAPFAMILMKMIGFKLTMSIACVTHFLGYLLASFATKIWHLYVCQGVIVGVSYAFLFVPASNVIPTWFLKKRAIASGISYGGTGLGGVIYSVSVNATIKRTGDQRWALRMVGLTTLFAMAISIFFIKQRKPFKREKLNFQNLKKNTQLMFSKKVLERKSLWYITLWFAMGIIGYNLVIFSYATYATTMGLSTSQASTLTALINAAQTLGRPMMGFVSDRWVGRINYSIVLDLLLTIFILAFWINAKTFISLLFCGLLIGATIGVGNVMNIVMIADSFNIEEFASAWAILNMGIGCFSLPVEVIALALRDYTISNPFLYTQIFGGLLFFISIVIIAPQREWAVRKSMKKESDQLIRELRDLEPVGSKDLHNVHVEDMTRELKKRSDKLEGLLQNNPGMYFRRLFYPMKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0V6</accession>
    <name>A0A1E3P0V6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_63407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038107.1">
        <property type="nucleotide sequence ID" value="XM_019183540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0V6"/>
    <dbReference type="GeneID" id="30200786"/>
    <dbReference type="OrthoDB" id="10474981at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020366">
        <property type="entry name" value="Ies5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17335">
        <property type="entry name" value="IES5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="193" mass="22183" checksum="4F3512D21047E88A" modified="2017-01-18" version="1">MSDAEPKSEITNNEPKSEISDFQPLKSLKKPKSYAKIYEKLSKKYSNVIGTSENLFSNYTNLRKEYISLSNKIGSITDILGLIDRLHDHQHDTSDSKERLQSIISQKPQLQKILGVLITDDGSFPKHLEELYPLTDLAVHLLQDHVEETDPQSRSAWIKRNHPELINGDFGTRKRRASTEDHDNESKKRIKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0W6</accession>
    <name>A0A1E3P0W6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_22953</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial ribosome (mitoribosome) assembly factor. Binds at the interface of the head and body domains of the mitochondrial small ribosomal subunit (mt-SSU), occluding the mRNA channel and preventing compaction of the head domain towards the body. Probable inactive methyltransferase: retains the characteristic folding and ability to bind S-adenosyl-L-methionine, but it probably lost its methyltransferase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038117.1">
        <property type="nucleotide sequence ID" value="XM_019180969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0W6"/>
    <dbReference type="STRING" id="683960.A0A1E3P0W6"/>
    <dbReference type="GeneID" id="30198215"/>
    <dbReference type="OrthoDB" id="421327at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180026">
        <property type="term" value="P:mitochondrial small ribosomal subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052571">
        <property type="entry name" value="Mt_RNA_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015324">
        <property type="entry name" value="Ribosomal_Rsm22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016522">
        <property type="entry name" value="RSM22_mit_bud"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184:SF5">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09243">
        <property type="entry name" value="Rsm22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007797">
        <property type="entry name" value="RSM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="315"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="185"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="620" mass="70301" checksum="9085DB7EDD6EA17A" modified="2017-01-18" version="1" fragment="single">LDDSELDSKIRVISREDYLAQTLERSPFRDEEGRPIQGSNAEEARLHPRTLIARNPREQIKVPDLISRAIKSHILALRLPKRLRTSVSDMYIALQKNQIHKPTKSAMECDAHIAGLFIQNYASIYQVLSELKKRTKDFNPNKVLDIGFGPATGMIALNQLMGNEFKPEVKDALIVGDKSMKDRAKILLSRQLNEYTGDIKDLELEMDEAQEEMLEDVDENVFQDEIDDRVGAIKTNHIKVKTKLRDYLSPTKKYDLIIATHQLLQDSSRFPFQVDENVDILLKSLSPNGHLVLVERGNPLGFEIVARARHVMIRPDRNGNENGKIPRSYQKNENTKGKNRSNLGKLSEEMEMAKELEERFGSLSEKDLEFEEGLEELEVEDFDKLASNIEESLETIDKDPYHISIVAPCPHHHKCPLQTSKPEYYTTPPGGKLNFCNHEVSVERPPFSLDVKRGKVLASKWGSPTAGRSSIGLPGSGRPNGKNYELASFSYMIAHRSPNDAETVSSIEKRREEATHDDLLGTEGEDQSTWPRIMLQPMKRTGHVTMALCGASGKIEKWTVSKSLKHQIYYDARKSAAGDLWAFGAKSKTVHDMGVNDTKIEKIENFYKNEAKRLASEKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P123</accession>
    <name>A0A1E3P123_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_79449</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038111.1">
        <property type="nucleotide sequence ID" value="XM_019184894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P123"/>
    <dbReference type="GeneID" id="30202140"/>
    <dbReference type="OrthoDB" id="4083131at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="164" mass="18589" checksum="542DAB1C85CB089B" modified="2017-01-18" version="1">MFKLNLFNTPFNFWKQAAAVNAPENTKQPINNNNKIDKTKVISMNHNEEGDFVEVDIKKLSYAEVAALSRTSKPHQIKHDGNKGNNNNKDKVDSSDVVDVNELEREVTDYQMSSVLSKNVLRKQVDALDNAVGDVDDESMGLDEEFNDKSNRRSKKSKFLKKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P137</accession>
    <name>A0A1E3P137_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_32144</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038250.1">
        <property type="nucleotide sequence ID" value="XM_019181921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P137"/>
    <dbReference type="STRING" id="683960.A0A1E3P137"/>
    <dbReference type="GeneID" id="30199167"/>
    <dbReference type="OrthoDB" id="5398391at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3330.10">
        <property type="entry name" value="Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018020">
        <property type="entry name" value="OHCU_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036778">
        <property type="entry name" value="OHCU_decarboxylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37987">
        <property type="entry name" value="CHROMOSOME 9, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37987:SF1">
        <property type="entry name" value="OXO-4-HYDROXY-4-CARBOXY-5-UREIDOIMIDAZOLINE DECARBOXYLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09349">
        <property type="entry name" value="OHCU_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158694">
        <property type="entry name" value="UraD-Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase" evidence="2">
        <location>
            <begin position="13"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="183" mass="21158" checksum="8D2419F2183F7DA2" modified="2017-01-18" version="1">MSYKLPDISTLPTLPQAEQDEVLTHLFEPSETLFTYLRPYLSTSYSSYIELIETSRTAFLKLVDDKERDDLIKDSRIGDIIACHPRLGVPKTTKLSEHSSKEQKSLQSDDETIKKFVTLNETYESQFPGLRFVCFVNGRPRDEIVGIFQDRIDKNDYKQEVIDALNAMCDIAIDRYKKIQPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1A9</accession>
    <name>A0A1E3P1A9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_69501</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038330.1">
        <property type="nucleotide sequence ID" value="XM_019184168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1A9"/>
    <dbReference type="GeneID" id="30201414"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="213" mass="24040" checksum="F293FBDF3FAA747F" modified="2017-01-18" version="1">MSSIESGNVDNMAQTNLLFGVASIIDISDINFDHGVLQLKQTAARLVARGIFQSDFKNAEYFVKTFNIDEYEFERVLHSAQGQIVNCRNELKSFLTRQTVSFDQAEHEFLSYGDLLGPTRTAKLAEIEGQEEETKDIVAWAVNRYLLTLKTSRGGDAQALEGYCKFTTELEKLCLGTFLLLKLYEGKPLTKTLCFHLLKTLCSNTKADLKYID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1G3</accession>
    <name>A0A1E3P1G3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">NADH-cytochrome b5 reductase</fullName>
            <ecNumber evidence="15">1.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_84498</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46680"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14 15">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="15"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037959.1">
        <property type="nucleotide sequence ID" value="XM_019185719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1G3"/>
    <dbReference type="STRING" id="683960.A0A1E3P1G3"/>
    <dbReference type="GeneID" id="30202965"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090524">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NADH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000032">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000009">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF171">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14 15" id="KW-0274">FAD</keyword>
    <keyword evidence="4 14 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="10 15" id="KW-0520">NAD</keyword>
    <keyword evidence="9 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="16">
        <location>
            <begin position="51"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ58752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="300" mass="33390" checksum="6F1009FF2B04B718" modified="2017-01-18" version="1">MASFLSRFTSQRATWVPIAVGSVAVAASLYYTNANKSPLANETPVTFKGDDQWIDLKLVKSVEISPDTRHFYFALPSADHKSGLITASCLLAKYVTEKGNNVVRPYTPISDVEQKGQLEFAIKRYPTGKFGNHLFSLKENDTVSFKGPIVKWEWTPNQFENVTLIGGGSGITPLYQLLHEITKNPEEKTKINLVYGNKTPGDILLKKDIDSIAEKYSDRVKVHYFVDKAEGDWKGETGFITKDWLNKNVPHPDASHQVFVCGPPPLYEAISGNKVSPSDQGELTGALKELGFTKQNVFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1V0</accession>
    <name>A0A1E3P1V0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_31613</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038515.1">
        <property type="nucleotide sequence ID" value="XM_019181859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1V0"/>
    <dbReference type="STRING" id="683960.A0A1E3P1V0"/>
    <dbReference type="GeneID" id="30199105"/>
    <dbReference type="OrthoDB" id="5290825at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015798">
        <property type="term" value="P:myo-inositol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015791">
        <property type="term" value="P:polyol transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000474">
        <property type="entry name" value="Sugar transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF25">
        <property type="entry name" value="SUGAR TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G05830)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="270"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="430"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="493"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="530"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="562"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="139"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="654" mass="73973" checksum="3767A4E230F18A8C" modified="2017-01-18" version="1">MPAEQFDEKTQIEQTSNIEANDSLTKESTAANPSGGIFKTNTSSSGTSSKEKAALTDGASPTEDQNILTQYDLEQVMNMGRNFALKHDLPDPELFARGAALARNPKDYNRLSFLSDEEKEAAYNEIHHPWRIPWKLIQIIFSISLCAATQGADETVINGALLFYPKYMGIANNDERSRWLEGLVNAAPYLCCAVIASFITDPANKRWGRKKVIFWSSFASSVTCFWQGFTNNWYHLFIARCCLGAFGIGPKSATVPVYSSEAAPAPIRGALTMLWQFFTAAGIALGYVFSLAFFHVPDHGIGGGLNWRLMLGSAMLPATLALFQIPFIPESPRWLMGKGRFAEAYKSLRSIRNHDISAARDLFYQYVLLQEEESFDAPTTQRLKELFTIRRNRNATIASFIVTFMQQFCGINVIAYYSSTIFVEANFAELNALGASLGFGVINTVFALPAFFTIDHYGRRFLLLMTFPLMSAFLFLTGFSFWIDKDTHPDGRIGSICLGIYLFTIFYSFGSGVVTFPYTAECFPLYIRTLGTSIMIGNLWFWNFILAITWPSLLEAFKPQGAFSWYAGWNIVGFFLVLWFLPETKSLTLEELDEVFDVPAMTHARYQTVELWHSFQIYILRRKDIVRQPPLHKHHRLAVTNTEWDDKPQTQQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1Z3</accession>
    <name>A0A1E3P1Z3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Elongator complex protein 3</fullName>
            <ecNumber evidence="15">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_69583</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalytic tRNA acetyltransferase subunit of the elongator complex, which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>uridine(34) in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O = 5-(carboxymethyl)uridine(34) in tRNA + 5'-deoxyadenosine + L-methionine + CoA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.3.1.311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="15 16">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ELP3 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="15"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038421.1">
        <property type="nucleotide sequence ID" value="XM_019184173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1Z3"/>
    <dbReference type="STRING" id="683960.A0A1E3P1Z3"/>
    <dbReference type="GeneID" id="30201419"/>
    <dbReference type="OrthoDB" id="10265243at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106261">
        <property type="term" value="F:tRNA uridine(34) acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140018">
        <property type="term" value="P:regulation of cytoplasmic translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000003">
        <property type="entry name" value="Elongator complex protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056591">
        <property type="entry name" value="ELP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23613">
        <property type="entry name" value="ELP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005669">
        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01086">
        <property type="entry name" value="elongater_protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13 15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11 16" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15 16" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 15" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5 15" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Radical SAM core" evidence="18">
        <location>
            <begin position="88"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="17">
        <location>
            <begin position="400"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR005669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005669-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ59214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="553" mass="63261" checksum="43E3F300BE21BEE2" modified="2017-01-18" version="1">MAVGVIKSKQKLAPEKERFLQCCSDITLELIDSLKTSKEINLNGLITKNSKKYKLKSQPRLTDVINSIPDQYKKYLLPKLKAKPVRTASGVGVVAVMSKPHRCPHISFTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPYEQARGRIDQLKQLGHSVDKVEYIVMGGTFMSLDKEYRENFITQLHNALSGYNGTDIDEAIAYSQQSLTKCVGITIETRPDYCTKTHLDDMLKYGCTRLEIGVQSVYEDVARDTNRGHTVKSVCETFAIAKDTGYKIVSHMMPDLPNVCMERDLAQFKEYFENPDFRTDGLKIYPTLVIRGTGLYELWKTGRYKSYNANALIDLVARILALVPPWTRIYRVQRDIPMPLVSSGVENGNLRELALARMKDFGTKCRDVRTREVGIQEVHHKVQPDQVELIRRDYYANEGWETFLSYEDPKQDILIGLLRLRKASKKHTYRKEFTKQRTSIVRELHVYGSVVPLHSRDPRKFQHQGFGTLLMEEAERISREEHGSEKISVISGVGVRNYYSRLGYELDGPYMSKSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2B7</accession>
    <name>A0A1E3P2B7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Altered inheritance of mitochondria protein 24, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_78276</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the AIM24 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038846.1">
        <property type="nucleotide sequence ID" value="XM_019184694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2B7"/>
    <dbReference type="GeneID" id="30201940"/>
    <dbReference type="OrthoDB" id="5295771at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.160.10">
        <property type="entry name" value="Mitochondrial biogenesis AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002838">
        <property type="entry name" value="AIM24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036983">
        <property type="entry name" value="AIM24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36959">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36959:SF2">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01987">
        <property type="entry name" value="AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="313" mass="35064" checksum="ABE89D89F8A088D6" modified="2017-01-18" version="1">MKPQLPLLNAQPTQRIIPQITKSSIIPLQPSQPIHIHHNKILSIKTESLSKISSKTQYNNPLGSLLYGGFYLKYRKFESIIPSEILLAGQNNNKLRLDGLIDWAIFPKDALISYSGVALNVSLHKIPWGSGLSSLLKLGYLHISGRGDVYLQNHGEIYTVNLGEKEEIIVRKNGVVGISVNGDELGSCCDAVALSNHEVQKESQEVVAGKSENELKDLFWNTYHGIGKTLRYLVGLIAKDDFVKVRGPRSLILTSNVSTKFEFPRRDNEIEITEQQLNKSSQDYLSYVTVKDGKVTFESTPSFKETVEKIERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2I8</accession>
    <name>A0A1E3P2I8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_91517</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038903.1">
        <property type="nucleotide sequence ID" value="XM_019186300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2I8"/>
    <dbReference type="GeneID" id="30203546"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="65" mass="7514" checksum="CF4E3E1D10260557" modified="2017-01-18" version="1">MQTYNYAKGNIALKGDMEASSGDQLIAYLLDDYKTILWIDFQLKVYKKYMLIIPFLKTGNMVIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2N1</accession>
    <name>A0A1E3P2N1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">pyridoxal 5'-phosphate synthase (glutamine hydrolyzing)</fullName>
            <ecNumber evidence="3">4.3.3.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_30969</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by a SNO isoform. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the PdxS/SNZ family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="3"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038717.1">
        <property type="nucleotide sequence ID" value="XM_019181781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2N1"/>
    <dbReference type="STRING" id="683960.A0A1E3P2N1"/>
    <dbReference type="GeneID" id="30199027"/>
    <dbReference type="OrthoDB" id="1660966at2759"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04727">
        <property type="entry name" value="pdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000001">
        <property type="entry name" value="Pyridoxine biosynthesis protein PDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01824">
        <property type="entry name" value="PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001852">
        <property type="entry name" value="PdxS/SNZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033755">
        <property type="entry name" value="PdxS/SNZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003215">
        <property type="entry name" value="PRK04180.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00343">
        <property type="entry name" value="pyridoxal 5'-phosphate synthase lyase subunit PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01680">
        <property type="entry name" value="SOR_SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029271">
        <property type="entry name" value="Pdx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51129">
        <property type="entry name" value="PDXS_SNZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0704">Schiff base</keyword>
    <feature type="domain" description="PdxS/SNZ N-terminal" evidence="11">
        <location>
            <begin position="5"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with D-ribose 5-phosphate" evidence="9">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR029271-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ59510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="294" mass="32182" checksum="47864E38B9E7B04E" modified="2017-01-18" version="1">MSDLKLNKGLAQMLKGGVIMDVVNAEQARIAERAGACAVMALERIPADMRASNQVCRMSDPKLIKEIMETVSIPVMAKCRIGHFVESQILEEIGIDYIDESEVLTPADKLYHIDKKKFKVPFVCGARNLGEALRRIDEGAAMLRTKGEAGTGDISEAVSHITLIKQEIEYVLTLKNDEVKLEVYANDNRIPIQRLKDLISNNGKFPVVNFAAGGIATPADAALCMQLGCDGVFVGSGIFKSKNPERLAAAIVNSVTHYNDPAKLLEYSTDLGELMFGVAVDQLKEKEKLSTRGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2N4</accession>
    <name>A0A1E3P2N4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_56196</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038671.1">
        <property type="nucleotide sequence ID" value="XM_019182773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2N4"/>
    <dbReference type="STRING" id="683960.A0A1E3P2N4"/>
    <dbReference type="GeneID" id="30200019"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000386">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000065">
        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF39">
        <property type="entry name" value="TRANSPORTER LIZ1_SEO1, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G00980)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="136"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="398"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="422"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="457"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="491"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="64206" checksum="012C0920ED9075A5" modified="2017-01-18" version="1">MALGYKNDYHSQVDHLVAGSDTDSNHGSNGHLKLDGEVDFRDEDEPYIDITVHLTPEELKRRSKWWYKASVFLWDSADKHPKERSFLFKLDFFLMSSAMLGYFIKTLNQSNISTAYVNGMKEHYHMKGNDYNLMNTLWTIGYIIGQIPSNLILHRISARYYLAGLEICWAFLTILMILPKSMSGLYAIRFLIGLTEAGYFPGLEYLIGSWYSSKELNKRSTLFNCAGTAAGLVSGPLQQRILHASFTEGSRWKPFQWMFIFDAVISAPIGFYTLFADPNTPSTTNAWYFSKDDKLVALERRRRIGAQLNTREPYTFKKVKSFFNTWHIWVFPWLFFCFRLINVAYSSNGFQLWMKMDLKLTPYQYNIYPTGINGGGIGFAIICAYLNDYFKNNLTQWFLFFMFISGIVSYAPLAVWDINVGFKWFCFFFYNIVSSCGQSMIFSWVNKNMAYDDMKRNFLVVTTNLVSYIFTAWISLVVFNQDDAPKFRKGYIFESCISVLGLILTATSWILIKRDDEKYLKEHGRPRDIHEGEAELIIEEGNSLEQGTTVSKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2P6</accession>
    <name>A0A1E3P2P6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SWR1-complex protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_84805</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SWC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038379.1">
        <property type="nucleotide sequence ID" value="XM_019185783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2P6"/>
    <dbReference type="STRING" id="683960.A0A1E3P2P6"/>
    <dbReference type="GeneID" id="30203029"/>
    <dbReference type="OrthoDB" id="19740at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032563">
        <property type="entry name" value="DAMP1_SANT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027109">
        <property type="entry name" value="Swc4/Dmap1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12855:SF10">
        <property type="entry name" value="DNA METHYLTRANSFERASE 1-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12855">
        <property type="entry name" value="DNA METHYLTRANSFERASE 1-ASSOCIATED PROTEIN 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16282">
        <property type="entry name" value="SANT_DAMP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DAMP1 SANT/Myb-like" evidence="10">
        <location>
            <begin position="169"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="127"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="358"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="483" mass="55749" checksum="DDCE18824ABD5188" modified="2017-01-18" version="1">MSSDLLDVLNLQQRDAPAKKKQKVAEPKVKQTGINRELYNLLGQNTPPVPVRNQQKFKDRLNSLTKPSPWTWASFKNGSRSDSLQLHHWVKGSKESVEAAEVPYKFEKYNTSLNIPSFTEEDYNNFIADEPIDGNNEMDIDENKAPDSKEEETSKKPETEDDETNTEKTASEVEEIVKQLPWDYEETRYLFDLSIAFDLKWVVINDRYDFKGNSRSIEDLQERFYSVCQRILIHENDKVEDTQNSNLISNLNFNKRKEIKRKMYLNRLLERSPAEIAEEESLLIEARKFEVSAKKTITERASLLQLLDSPQSNGSINQYLSSQGLTQLYNSLMTEKRKRRVDSPAPENPLTALNEKIKQQHIQRQKQIQQEKLEQQKKKKENPISTLLSKNLTPREEEEFGLKIHAEKISPGVALRSTKLSTFKPGVQAKITTILNEMGLGKKPSLATSRVVAKQEELYKTINILLELKRQVDKLEAEAKIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2V4</accession>
    <name>A0A1E3P2V4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_78455</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039028.1">
        <property type="nucleotide sequence ID" value="XM_019184722.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="30201968"/>
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    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="225" mass="26657" checksum="02BA02387740D1A3" modified="2017-01-18" version="1">MSELIQVQNNGVSDDSYMDVLREERESLEESFEATIKIIDQLIEFYSLDPTDETNISKYQDLIRTLSKDPSVIFRHSKNIDRLEKSFQRLYRERLDELSKINLHSFKDVSTNLETLYDQNLVNNEIETLERDLGDLKPIELDSDKVLTLDLSQDQLLEVLKIEKDKRARFSFLNERVHEPEIKKLEGSYDKWSEREKDLKRFLTVDLEKINKQIQNVKKTEGLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P310</accession>
    <name>A0A1E3P310_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 5B</fullName>
            <ecNumber evidence="3">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Translation initiation factor IF-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_100127</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="3"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038940.1">
        <property type="nucleotide sequence ID" value="XM_019180288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P310"/>
    <dbReference type="STRING" id="683960.A0A1E3P310"/>
    <dbReference type="GeneID" id="30197534"/>
    <dbReference type="OrthoDB" id="4928at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03703">
        <property type="entry name" value="aeIF5B_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000013">
        <property type="entry name" value="eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000026">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000112">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029459">
        <property type="entry name" value="EFTU-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003078">
        <property type="entry name" value="PRK04004.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14578">
        <property type="entry name" value="GTP_EFTU_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="15">
        <location>
            <begin position="408"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="121"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="135"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="163"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="263"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="276"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="300"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="338"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ59733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1007" mass="112240" checksum="3A0A7EF225584585" modified="2017-01-18" version="1">MAKKDKKGKKGGDYWDDEFEDDAPQAEEFGSTPAADESAEATPGATEATEDDVAGDFMATLRKNKEKKAKQTDEKPKNSGASVIKSKKEKEKEKKEKEKQRKKEMAAKKKAATAGNKKDNSAASSNASTPAPESAPKDEPEVPKAEPKGKGKKVPAGIAALRRQLELKKQAEEEERRLQEEEDKLAKEEEEKLAQEEAEKEAARAAKKEKERLKKEQLRAEGKLLTKKQKEEKKAIERRRQQLLNSGVIVEGLQKSGEEAPKPKKVVYGKKKKPTKAQEEEKAAAEAKAKAEVEAKANQDDEDEALVEDWENMALDDDEPLAEDWEAAGNEDESKAEEQEEEIAEVDEEQEAEADSGETEEEVKAKEAAKQKAELEAKAKEAEQKIKEVEQKVSKRNQKQSSSSQADLRSPICCILGHVDTGKTKLLDKIRQTNVQGGEAGGITQQIGATYFPIEAIKSKTAVMAAYEKQTFDVPGLLVIDTPGHESFSNLRSRGSSLCNIAILVIDIMHGLEQQTIESIRLLRERKAPFVVALNKIDRLYDWQTTPNNSFRDSFSKQTRSVQQEFDDRYTQIKVALAEQGLNSELYFQNRNMSKYVSIVPTSAVTGEGVPDLLWLLLELTQKRMSKQLMYLSEVEATILEVKVVEGLGTTIDVILSNGYLREGDRVVLCGMNGPIATNIRALLTPQPLRELRLKSEYVHHKEVKAALGVKIAANDLEKAVAGSRLLVVGEDDDEEELMEEVMDDLTGLLDSVDTSGRGVTVQASTLGSLEALLDFLKDMKIPVMSIGLGPVYKRDVMKATTMLDKAKEFAVMLCFDVKIDKEAEQYATENHIQIFSADIIYHLFDAFTAYQNKLLEQRRKDFLEYAIYPTVLKTIQIINKRNPMIIGVDVVEGSVRVGTPICTVKLDPETKQKTTMVLGKVVSLEVNHKSLEIVKKGQTNAGVAMRLENPSAAQPVWGRHVDEKDPLYSLISRKSIDTLKDPAFRDQVARDDWLLIKSLKPIFDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P330</accession>
    <name>A0A1E3P330_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TMEM205-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_78254</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038821.1">
        <property type="nucleotide sequence ID" value="XM_019184691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P330"/>
    <dbReference type="GeneID" id="30201937"/>
    <dbReference type="OrthoDB" id="1641132at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025423">
        <property type="entry name" value="TMEM205-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053009">
        <property type="entry name" value="Xanthocillin_Biosynth-Assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23241">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PLANTS LEA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23241:SF102">
        <property type="entry name" value="LD23009P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13664">
        <property type="entry name" value="DUF4149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM205-like" evidence="6">
        <location>
            <begin position="12"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="159" mass="17733" checksum="8757C2F1D80C1BC0" modified="2017-01-18" version="1">MSLFRPTAHLFIWSLVWGGSGYYSYVASPIAFKTLSRKEFSALQSKIFPHFFLAQSVSPLLIGLTAPYALTTGALVTLGTAVIGGLTNLLWLLPSTRKIKEARFKLEDEGKEDSVEHKELSKQFGKIHGLSLLFNFVNFAALTTYGFILTKNIIRYVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P366</accession>
    <name>A0A1E3P366_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWR1-complex protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_91646</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Involved in chromosome stability.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SWC5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038965.1">
        <property type="nucleotide sequence ID" value="XM_019186308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P366"/>
    <dbReference type="STRING" id="683960.A0A1E3P366"/>
    <dbReference type="GeneID" id="30203554"/>
    <dbReference type="OrthoDB" id="445677at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011421">
        <property type="entry name" value="BCNT-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027124">
        <property type="entry name" value="Swc5/CFDP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48407">
        <property type="entry name" value="CRANIOFACIAL DEVELOPMENT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48407:SF1">
        <property type="entry name" value="CRANIOFACIAL DEVELOPMENT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07572">
        <property type="entry name" value="BCNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51279">
        <property type="entry name" value="BCNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BCNT-C" evidence="5">
        <location>
            <begin position="215"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="178"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="131"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="295" mass="34314" checksum="BF58FDF497FB8535" modified="2017-01-18" version="1">MSQELAEKKEQEEEYIEEEDEDFDPENAQDDNFSSDDEEEVKPTKKQKKNDKNGGDDYDLSTEKEVKSANYDRIESAEGGLIKTRTQRALEEQQAKQYKTVSGVKSNVDIDDVWKNLQAQSKERLKYRYGSSSPSPSQTPQQLNSNEKIKIKRRYEFAGEIVVEDKWVDRESAEAREYLNSVKNNPNEKVETQEGDDKPKEQKSNDVGKKLRVKRKRPPLLEGIISGAIKPKFNTLEKSKLDFAQFVDKEGIDDELRQHNKNGYLEKQDFLSRVEFFKDTQIKEMRKKELANQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3I0</accession>
    <name>A0A1E3P3I0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SART-1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_79949</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNU66/SART1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038644.1">
        <property type="nucleotide sequence ID" value="XM_019184981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3I0"/>
    <dbReference type="STRING" id="683960.A0A1E3P3I0"/>
    <dbReference type="GeneID" id="30202227"/>
    <dbReference type="OrthoDB" id="5583at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000481">
        <property type="term" value="P:maturation of 5S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045347">
        <property type="entry name" value="HIND"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005011">
        <property type="entry name" value="SNU66/SART1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14152">
        <property type="entry name" value="SQUAMOUS CELL CARCINOMA ANTIGEN RECOGNISED BY CYTOTOXIC T LYMPHOCYTES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14152:SF5">
        <property type="entry name" value="U4_U6.U5 TRI-SNRNP-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19252">
        <property type="entry name" value="HIND"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03343">
        <property type="entry name" value="SART-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="256"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="391"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="565"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="265"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="287"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="396"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="423"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="565"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="586"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="626" mass="72202" checksum="C564313D240A6D5D" modified="2017-01-18" version="1">MSEESISIEETNRIRVSLGLKPIPLPNETKQAPKNDEISLDDTNKLRVSLGLKPITKDNTSNPNEKSSEEYQQEIRNREKEEKIKKNLEIAKSRAAKRKKINISKSLLDDNDQDDDGDDWLSRIKKPSEVKKVKKVKRARVDEDGGMSGVKVGHSIDDINQLQQEDVILTLKDRSIHDDEDELESDLLVKKQKLEKQMAEKLRTNKYKKFNGLESEEKTLLERYNEELNGENGESFILNGETIKVDKSKEVEAVEKKGKRRMEFSLDDDDDGDEDEIMGNSDYAKAKPIKMKKLKKKSSNGRKKEERKDEEGDVVALESVELKDEDSSIQDDFELQNALALKRLKNHRKRKYLKPEDIAKEINEDKENGMNVDKVEQGIVIDENTEFLSSIRARNDEEEPKDEEIVQDAEPIQDTNEDKMDVEPSEPLDNETVEDEEDAQDVNFNGGIGSLLGFLKSKNIVQEKTSDQIESDRKAKELKKKLDLEKLKTEIEKRIYEENLREDSSFKKLSKKEREEVLESELQQFKDLQNSEEVASKLENYKPQVKLRYIDEYGREMNTKEAYKHLSHQFHGKGPNKSKIAKKLKKVEEERKQQYKENSLLGGLSNDNKGDQDSSASQDKVGVRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P442</accession>
    <name>A0A1E3P442_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DAGKc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_78875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039473.1">
        <property type="nucleotide sequence ID" value="XM_019184802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P442"/>
    <dbReference type="STRING" id="683960.A0A1E3P442"/>
    <dbReference type="GeneID" id="30202048"/>
    <dbReference type="OrthoDB" id="3853857at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001727">
        <property type="term" value="F:lipid kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF108">
        <property type="entry name" value="DAGKC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAGKc" evidence="1">
        <location>
            <begin position="63"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="406" mass="44861" checksum="153F0AB184B1EAE0" modified="2017-01-18" version="1">MSEPKKQEFLFQLKSGTQSKSYSLAYEETEAANVNFELLSSVGADGKKDELVISKDNLPTHLKPGVDVTVIDSVNSGVGREKREFYTSVLEPILNELDIKHTLLKTTSALSITNFAKSLDLSKDHTVVLLSGDTSVVEFINGLPTDANVSTKPNINLVLIPLGTGNALVSSSGITSEANAVQKMFSNEKAELPLYEADFPSESFAVHLENAPPIGKLLFTIVLSWGSHAQMVYHAESPELKGLGIERFKIAAYKIFQQKLEYNCDIIIVDENGKEEKMSHSTTHNYSSILAVPRLEKKYHISPDSKISKSELHILDFGDLPKDEFMGLLMEPYKGAGHVNHKGVIYEPIQPKKQIILELGEEDEERSIVCVDGISIKVKNGKGKRITVRFVNSEDLSFKLNLVGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P468</accession>
    <name>A0A1E3P468_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_16128</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Required for assembly of the mitotic spindle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 14">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038899.1">
        <property type="nucleotide sequence ID" value="XM_019180675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P468"/>
    <dbReference type="STRING" id="683960.A0A1E3P468"/>
    <dbReference type="GeneID" id="30197921"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903754">
        <property type="term" value="C:cortical microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061863">
        <property type="term" value="F:microtubule plus end polymerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000073">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF36">
        <property type="entry name" value="KINESIN HEAVY CHAIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 14" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9 13 14" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="16">
        <location>
            <begin position="27"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="522"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="99"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ59692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="560" mass="62538" checksum="7E1727973018E433" modified="2017-01-18" version="1" fragment="single">PGSSLSSSTSRPQTPSFITPSQPYTGSISVAIRAKPSDTFLKDPWFLTNDSIQHNEIGDFKFDHVFHPSVSNYEVYDKVVKNLIKSLMDGYNATVFAYGMTGSGKTYSMSGIKGEPGVIPLSINEIFNHIENADKEIYKHELKVSYLEIYNEKIYDLLNINQLTNPALNNTQTELKIRDVPNYGVKVIGLVEEKVNSEHELLNIIQKGDLNRRTGETDFNTRSSRSHAIVLVRVCTTNTVTGIETFSTLSLCDLAGSEKATAQHERRKEGSYINKSLLALGSVIVKLSSSTTSAGHIPYRDSKLTRLLQPALSGDSLVSVLCTIHTGQNALAETVNTVRFAARAKNISLTVRKHEIDLTSEKDRMIEKLKSQVQIQAEELLLLKSSKGTSIGDENIDSGDASNQVSKEEVAQLSAENRILVERLEHYKRLNDESNAGRVVLKNDIVNDILTKLSNQDMDAANEELILKVEELFKKTYSEIDEYKSYINHLEHQLKTEVQNQSSGKRVKVGHQAFSPSKHASANEVDLILKDQEDEIFELKAELKNKEKIIKALQSAKRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4H4</accession>
    <name>A0A1E3P4H4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Carbohydrate-binding module family 48 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_92764</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 5'-AMP-activated protein kinase beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039578.1">
        <property type="nucleotide sequence ID" value="XM_019186378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4H4"/>
    <dbReference type="STRING" id="683960.A0A1E3P4H4"/>
    <dbReference type="GeneID" id="30203624"/>
    <dbReference type="OrthoDB" id="531008at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140767">
        <property type="term" value="F:enzyme-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000562">
        <property type="entry name" value="Snf1 kinase complex beta-subunit Gal83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006828">
        <property type="entry name" value="ASC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037256">
        <property type="entry name" value="ASC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050827">
        <property type="entry name" value="CRP1_MDG1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF84">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04739">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01010">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160219">
        <property type="entry name" value="AMPKBI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Association with the SNF1 complex (ASC)" evidence="6">
        <location>
            <begin position="267"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="206"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="210"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="380" mass="43216" checksum="86767EA4EC87652B" modified="2017-01-18" version="1">MGHNSSDYNREDENPITQHNENSQSNQDVTMSDSGASNVNLNSQPSAMNIDSELNPEQEQEQAEPEHQPTPRRRSTMILTDDPEFEKLKTKSANLDINNEESISKLEDHNNSQDSQHNNNTIPFEIVWVQGGENVFITGSFTGWRKMIKLTKETDEKFSIKLKLPVGTHRFRFIVDNELRFSDYLPTATDQMGNFVNYIEIQQPDVQMGQDDSQQNEYTQDQSQGQEHGEQKVGKPSDEDRRDSNLGLTNDDDDMGNGYSRFHEEQSSKPNLNYIKDIPAIFTDPSVMEQYYLTLDNNAQNNQNQSWLTPPQLPPHLESVILNNFSTTDKSNSSGALPIPNHVVLNHLATTSIKHNTLAVASVVRYKRKYATQVLYAPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4K4</accession>
    <name>A0A1E3P4K4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="24">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">WICANDRAFT_30420</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 24">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphocholine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38583"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:38584"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(out) + ATP + H2O = a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66036"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:66037"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 23">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Membrane</location>
            <topology evidence="24">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 24">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="24"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039019.1">
        <property type="nucleotide sequence ID" value="XM_019181731.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P4K4"/>
    <dbReference type="GeneID" id="30198977"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140351">
        <property type="term" value="F:glycosylceramide flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090554">
        <property type="term" value="F:phosphatidylcholine floppase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140346">
        <property type="term" value="F:phosphatidylserine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090556">
        <property type="term" value="F:phosphatidylserine floppase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000054">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000087">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000108">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF180">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE DNF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 22 24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="11 23 24" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 22 24" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 24" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="384"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="428"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="1008"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="1036"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="1087"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="1126"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="1153"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="1193"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="25">
        <location>
            <begin position="38"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="26">
        <location>
            <begin position="973"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="21">
        <location>
            <position position="500"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="500"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="500"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="501"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="502"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="502"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="634"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="675"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="697"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="733"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="813"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="814"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="815"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="921"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="927"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="947"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="950"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="951"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="951"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
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            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF16212"/>
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    </evidence>
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            <dbReference type="EMBL" id="ODQ59812.1"/>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1301" mass="147535" checksum="1FD1E9F259DC0CD0" modified="2017-01-18" version="1">MGVAETIHGWFVKPRVLEPRTVYFNQELPPSEIDQKSHRPLKIYDKNEIRTTKYTPFSFIPKNLFFQFQNIANVYFLFLIILGAFQIFGVQNPGLAAVPLCVIVVVTAIKDAFEDSRRTISDLEVNNNPISILQGVENYNVDEKNIPFRSKLSNFNSSIFVWILNTFGKLFKKKQIIDTSTEPRGSLETYHSSLRPSLDNTNTNDIPAGVDHILNKNQTNASQPRFKKSYWKNVKVGDIVRIRNNEEAPADVIVLSSSDPDNRCFIETKNLDGETNLKSRQGLKSTSQLKHARDLAQCNFHIKSEAPSMNLYNYQGSLNWEDNEKSDSSDETINITNMLLRGSTLRNTKWAIGIVIFTGHETKIMLNSGITPSKRSRISKELNLSVLINFALLFILCLVSGIVNGTFYDRKDTSFKFYEFKAYGSTPAVNGIISFFVAVILYQALVPISLYISVEIVKTLQALFIYCDVNMYYEKLDYPCTPKSWNISDDLGQIEYIFSDKTGTLTQNVMEFKKATINGKSYGLAYTEAQMGMDKRKGIDASSEAVKWKKVIDEDRDKMIQILKESPENTHFDPEKLTFISSEYLTDLDNKEDKAQSEANHHFMLCLSLCHTVLTEPLKENPDQFEFKAESPDEAALVQAASDVGFTFIKRTRSGGIVNIQGTEKSFEILQILEFNSTRKRMSVITELDDEIFLISKGADSVIFARLDPNENDEVLLNKTAEHLEEYASEGLRTLCIAGRKLSKQEYQEWSKQYDAASSSMEDRDEKMEELASEIESNLVLMGGTAIEDRLQIGVPESIETLSKAGIKLWVLTGDKIETAINIGFSCNLLGNDMELLVIRPDEGKDPKQDMDSKLTEYLQKFNATGSIEELKKAKTDHSIPEGQYAVIVDGAALSAIFEDEDLQRKFLLLCKQCKSVLCCRVSPAQKASVVKLVRDALDVMTLSIGDGANDVAMIQTANVGVGIVGEEGRQAAMSSDYAIGQFKFLTRLVLVHGRWSYKRLAEMIPSFFYKNIAFTMTLFWYGIYNDYDGSYLFEYTYIMFFNLAFTSLPVIFLAVLDQDVSDKVSLKVPQLYMSGILRKEWSQYKFIYYMIDGLYQSVITFFFPYLVFYKGDIASYNGLNVDHRFWIGIYVCHISVTSCDIYILLRQYRWDWLTLLINSLSILVVFFWSGVWSSSTFSGELYKAAAQVYGQTSFWACFFVGVLVNVLPRFVFTTLNTFYRPGDIDIIRECAVRGDFEDTDSSKASLSDEEKDISSNNMTSTLSRMSLDRVHTTHEIPGLTQAHSLVKTFTQASSVRNAHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4X7</accession>
    <name>A0A1E3P4X7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_84179</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ribonuclease III family. Mitochondrion-specific ribosomal protein mL44 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039491.1">
        <property type="nucleotide sequence ID" value="XM_019185659.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3P4X7"/>
    <dbReference type="GeneID" id="30202905"/>
    <dbReference type="OrthoDB" id="67027at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:ribonuclease III activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19873">
        <property type="entry name" value="DSRM_MRPL3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044443">
        <property type="entry name" value="Ribosomal_mL44_DSRM_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044444">
        <property type="entry name" value="Ribosomal_mL44_DSRM_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207:SF32">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="RIBONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22892">
        <property type="entry name" value="DSRM_MRPL44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="10">
        <location>
            <begin position="33"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="9">
        <location>
            <begin position="218"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00024034"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00035187"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="314" mass="35109" checksum="000256C88B4E5B2E" modified="2017-01-18" version="1">MNRLRNPFILARSLGFQNLKVLTEEEASQSALLVALHKRLELPKEFKLSTLARCLTCRSSQNKLVDNYGLNILGKNLLSYYVSEHLLVQYPRLPTVILNTAINSYISAESLASIGEHAWGIEVDSTSSLQKYLSEEPIEYSYGKLRFVPVNSQSEKGVTKLSGGKHEYETKTAYGLATRSIIGALYASTKSEQATKKFINDHILSRKLDVSKMFEFEQPTRELSRLCKREGFEVPIARLLAENGRTTKNPVFVVGVFSGKNKLGESFGSSLKEAKIRASVNALKNWYLYKPLDPKVPSDESYKPGLVDHGAVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P511</accession>
    <name>A0A1E3P511_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase</fullName>
            <ecNumber evidence="4 14">4.2.1.134</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_83938</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:45812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:83728"/>
            <dbReference type="ChEBI" id="CHEBI:85440"/>
            <dbReference type="EC" id="4.2.1.134"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endoplasmic reticulum membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the very long-chain fatty acids dehydratase HACD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.134" evidence="4 14"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039134.1">
        <property type="nucleotide sequence ID" value="XM_019185625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P511"/>
    <dbReference type="STRING" id="683960.A0A1E3P511"/>
    <dbReference type="GeneID" id="30202871"/>
    <dbReference type="OrthoDB" id="46988at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102158">
        <property type="term" value="F:very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030497">
        <property type="term" value="P:fatty acid elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007482">
        <property type="entry name" value="Tyr_Pase-like_PTPLA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035:SF3">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04387">
        <property type="entry name" value="PTPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 14" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7 14" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ59927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="227" mass="25454" checksum="8680709A0FCC2204" modified="2017-01-18" version="1">MSTEKKSQSANLKQKRSQGWLVLYNQASASLWSIVLFNTVFLSILVGQPDLFKTTNRITTIIQTLALVEVYNSAVGIVRSPVLTTVSQVASRLLVVYGIFQVLPNSPANSHWAYITLSLSWSITEIIRYLYYAQNISSNGQPAKILTILRYNLFFVLYPSGVASEIGMIVLSLGEAEKVVGAWYKWFLIVIIGVYAPGFYTLFTHMLKQRKKALKALKEADHPKKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P598</accession>
    <name>A0A1E3P598_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_77228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039750.1">
        <property type="nucleotide sequence ID" value="XM_019184520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P598"/>
    <dbReference type="GeneID" id="30201766"/>
    <dbReference type="OrthoDB" id="10568794at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="632" mass="72679" checksum="27276FCF94D15845" modified="2017-01-18" version="1">MPVYQPNLSRSNLPQGINSNNNYNNSENNNNSRPFVTTDATGRTVTINVAQDHLVPNTTSQQQQQQQQGNVSDYSFDDDDEDEDEDSVDDFRLRYLNLRGDTDDAGANTYNYSYNSSENSRRLNRPYLNPNNNFINSGSSSSRRTNNNNNNNNMSSNDRFRQAMLTPLPPPPPPQRRGTFNSEIPNSASSTLSVNRDTGNVSLSTYTNNSINLNNSILRRVMHPNNRFNNHQQFSDLMENNNNESHIRQEDFLNNTHNNTNNGGNAATFSLLSDINRYSTQSFYNDLNSQGHDPQEEPSNQQQELPMNYLFNHQEHSFNINRASQNINLDLNEFLAKRREVGDYYNKHLKFKAENPKKRDASQLSEGSVTTHYEGFHFNMIRVKSCSFLKPGLSFMVKNLKSDQIPFTIAFISVDYQKNLVRGSLNFEHLYLMKHWLSENAPDNAFINISSNNTVQFSGELIDFENTDLRYNSYSLESFNNSIADDIQNHSKNFRLIYNNCITRKQLSRWKKLKPFQNLNVKDMIPLITCNECLSKLQENFLLMKIYIDCHNSDNDDQLFNEVEPTILICVDRRTGELELISAEGKAKTIAEQYASNLNNEINDQKVNNSEDTVTLMSMKCKKYVSPSLQFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5D7</accession>
    <name>A0A1E3P5D7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Arp2/3 complex 34 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_30432</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Arp2/3 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
            <location evidence="1 6">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ARPC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039651.1">
        <property type="nucleotide sequence ID" value="XM_019181733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5D7"/>
    <dbReference type="STRING" id="683960.A0A1E3P5D7"/>
    <dbReference type="GeneID" id="30198979"/>
    <dbReference type="OrthoDB" id="148331at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072697">
        <property type="term" value="P:protein localization to cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1460.20:FF:000003">
        <property type="entry name" value="Arp2/3 complex 34 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007188">
        <property type="entry name" value="ARPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034666">
        <property type="entry name" value="ARPC2/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12058:SF0">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12058">
        <property type="entry name" value="ARP2/3 COMPLEX 34 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04045">
        <property type="entry name" value="P34-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69645">
        <property type="entry name" value="Arp2/3 complex subunits"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="312" mass="36880" checksum="2CABB944E2CCB04C" modified="2017-01-18" version="1">MLQLQSDNLLIKKTLTELFETPQNVDRIVSDFDYTTYHLSTPESKTKLLVSINSKSWKDLSKYDVTGKLNSIYGQFQTDTVEPGYNYSIYLDLEEIASKSKEEQLEIIDQISLLKRNTLAAPFEQAFDEYEELNAKYQETGIEPENSSSIIEINYRDEESIYIKPSFDRVTVIFSTIFKDETDKIFGKVFLQEFVDARKRAVQNAPQVLYSHREPPLEIRDLIKFSPNEQKGYVTFVLFPRHLVQQKRYNTISHIQLFRNYFHYHIKCSKAYMHSRMRFRVNQYLKVLNRAKPENDEAVERKTATGRRFLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5K3</accession>
    <name>A0A1E3P5K3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Spindle pole body component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_77209</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039731.1">
        <property type="nucleotide sequence ID" value="XM_019184517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5K3"/>
    <dbReference type="STRING" id="683960.A0A1E3P5K3"/>
    <dbReference type="GeneID" id="30201763"/>
    <dbReference type="OrthoDB" id="5860513at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008275">
        <property type="term" value="C:gamma-tubulin small complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005822">
        <property type="term" value="C:inner plaque of spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005824">
        <property type="term" value="C:outer plaque of spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF33">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="7">
        <location>
            <begin position="92"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="6">
        <location>
            <begin position="378"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="747" mass="87431" checksum="131E01A6922D772A" modified="2017-01-18" version="1">MTGGIEDTPRPRNPTVERITNQFVRENIDADGRSVSGSFFGGDSFENINFDRFSDRRSIYSAAQHRNDSRDYTLEEVAIPYFENHIHQDDILKYISFTLVGTTSNLFPLNNGEIELPDNLSNSESGLLHIVFECGLIYQFLVSLIENFKSNKSSSPTKNAIMSYIYEELTNYMKMVNILSNKQTFSLRSLFADLLDELVKLRFLYFISKRVGNEPGNKLLSLVYRYQNFGDHTVSESSSNLLKYSLEPFLKCLASWSVDGELIDKSNEFFVSRSDSSSKDIEVRFEEDKVPSFFSKELGFQIYLIGKTNIFLKKYCKESKWCNLFSQKLNIIFNRVRLNKIALFNDNEFQKSINDHYSEIINYFNYVIYTKFNFMSILESLKEFLFMGKGDFIQALVTNGIELLNEPSNSLSGHQLTKLLQSAVFGTTVRYHLVFSDEHDLLKNLDARLLEIGHGNVGWDVFTLDYQIQSPISLILNDELNQFKKDYLKIFNYLWKLRRLETLFIDEWRKEKNQRWRQSTSFGRSLFKKINRIRLVQNFLRDFFRNIEKFILEEIIETEYKELCDQLRPKAKKLRVPVVKTGNDFKVARGILSPSDHYLDEMNKFRLTFERKEHGYEEHSIDDLKNIHTHYLNSITKHKLFNGSSAGSKGTVSKKYYINQVNSLINLVFKFVITVKEFNMLVDEFEGHSRFGEEYSESANISGRFSTIYNNLLKLFDDFNYELKIFINDLSNDDDLKMRYLGISLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5N9</accession>
    <name>A0A1E3P5N9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_28570</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039968.1">
        <property type="nucleotide sequence ID" value="XM_019181506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5N9"/>
    <dbReference type="STRING" id="683960.A0A1E3P5N9"/>
    <dbReference type="GeneID" id="30198752"/>
    <dbReference type="OrthoDB" id="7042322at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43510">
        <property type="entry name" value="AMINOTRANSFERASE FUNCTION, HYPOTHETICAL (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43510:SF1">
        <property type="entry name" value="AMINOTRANSFERASE FUNCTION, HYPOTHETICAL (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="3">
        <location>
            <begin position="54"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="370" mass="41178" checksum="971ABB4928F74F84" modified="2017-01-18" version="1">MVFQNPFKVEQYMDEHETNITYNLGETCCHSLSLKDIEQLSGNPPPLDKIDSIRLTYGAIRGTDDLKNGISSLYDNIGLDDIVVTNGAIGANFLTIYSLIDSTDHVIVVDPTYEQLKSVPTIFKGEVSLLPLKSEEGWIPSVDTLKSLIKPNTKLIIINNPNNPSGQYIQTSILEKIVNVAKENDAYLLCDEVYRPLFHGVSKPKSIVELYEKGISTGSCSKAFSAAGIRLGWIASKDKDFINACFLRRDYNTISISMIDDIIGTYILLNKDAILKRNHELCAQNIKIIEDFVHRNSTKVSWVKPNSGATCFIKLHTVENTYDFCSNLAVQHSTLIVPGEVFDHPGYIRIGFGNSSKELVEGLKILEALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P619</accession>
    <name>A0A1E3P619_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_61444</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040088.1">
        <property type="nucleotide sequence ID" value="XM_019183175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P619"/>
    <dbReference type="STRING" id="683960.A0A1E3P619"/>
    <dbReference type="GeneID" id="30200421"/>
    <dbReference type="OrthoDB" id="9936937at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058841">
        <property type="entry name" value="HTH_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040554">
        <property type="entry name" value="KPWE_PEX14_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36855">
        <property type="entry name" value="CHROMOSOME 10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36855:SF1">
        <property type="entry name" value="PEROXISOME MEMBRANE ANCHOR PROTEIN PEX14P N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25871">
        <property type="entry name" value="HTH_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17733">
        <property type="entry name" value="KPWE_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PEX14-like helix-turn-helix" evidence="3">
        <location>
            <begin position="5"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Peroxisomal membrane protein PEX14-like KPWE" evidence="2">
        <location>
            <begin position="95"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="193" mass="22216" checksum="CABC98A8CD0E87D6" modified="2017-01-18" version="1">MEGQEQVYLQFEAFDFENEDFKNGLENVYESYLDHINQNNDGKINEIVTEIKPEEKQRLEVQAKTFYFCQQTGNILNIEDYEEWKKTRETAEPEYSSNYQELVELILAGKEIPGIKQIPDTVLEGETTEHKAEVRKKPWEIRREQEEKARAEAAAATAAAAVDAEGKEGSENVKDEDFGEENTLDEESKIAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P645</accession>
    <name>A0A1E3P645_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">DNA damage-binding protein CMR1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_84283</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-binding protein that binds to both single- and double-stranded DNA. Binds preferentially to UV-damaged DNA. May be involved in DNA-metabolic processes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the WD repeat DDB2/WDR76 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039619.1">
        <property type="nucleotide sequence ID" value="XM_019185683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P645"/>
    <dbReference type="STRING" id="683960.A0A1E3P645"/>
    <dbReference type="GeneID" id="30202929"/>
    <dbReference type="OrthoDB" id="9890280at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000001">
        <property type="term" value="P:regulation of DNA damage checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050853">
        <property type="entry name" value="WD_repeat_DNA-damage-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14773">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14773:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="324"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="38"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="82"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ60412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="516" mass="59699" checksum="DBF2CA3A3DF6CA14" modified="2017-01-18" version="1">MGLSEFEKQRLENIERNKKLLSSLNLNSISHEIYQDIKKEEQHDLKRRKTSSPKPKKPRDKQSIPLPTRRSRRLAGVSLDDGSDKSREFELEEEKRRKEKEELEKLKTIRVNGDLSLLDLIKDDDDEVKTEDGNDKLLTKFQSFLNNNGNFSIGDYYETIMAKDNSSKDVKNLREEFQSKTIYPHFKPNEIKLTKERMTYIAFHPSIDKKLIIGGDTNGNCGIWNSENTEEVDITSFKLHGKSICKYEFNINEPNKVYTASYDGSIRSIDLSTLKSTQFFINDFDGGISDINFNNQHEIYYTTLQGEFGRLDLRSNDHSKRKILRLSNKKIGGFTINPNDPDQLATASLDRTLKIWDLRNIVDCDWNEWDDELQTLHSFGTYDSRLSVSTVDWNHSNDLVCNGYDDTVNIFNLDTSNWSKDTNEDLTPNHKIKHNCQTGRWVSILKAKWHKFPVDKTEKFVIANMNKYYDVYDRNGVQLAHLSDPLMTVVPAVCNFHQTENWLVGGGASGKVYLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P678</accession>
    <name>A0A1E3P678_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_77530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040065.1">
        <property type="nucleotide sequence ID" value="XM_019184567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P678"/>
    <dbReference type="GeneID" id="30201813"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="456" mass="51720" checksum="B10082306F9E33C2" modified="2017-01-18" version="1">MSANSIIQFYLNTANTTANSSQHDLSKVKPSQLPASPEMATTPASLLKTNNNNIIFKKIEQLPTPAAESNLSVSPGEQQAKTGSGVPSFFSIPLRFFASKKSTPKLSDDELDKEIDDFKKASPIDVIKDSPTDIFSDQINTGDEVNQTDSVERTNSNMLKSENDGTARQSDLLSGDYDPIYDTYITYINHTTPEGDLILGEEPKQDVDDANPIPNNETLQPVANPHNEIKPIYQPEYDAYENIQLEFGISTLKRIKTRTKEILRQVSRHYSNVRDKLYEDHTPADNNETPSPPQIEPSTFRSELKRRLSSVSIRRSSLSIRRHNSGPKWKWPRRNSESNQLNTIPSSDNSPLKTVYNTFSHKGEHSKNQTLSELFQSADFESRLNEGSVSGRKSYQEDENVEDEYHISYRENNELKPTCSNNGLNTFQERLVHFKEQTLQRSKSFQTLAKYFTQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6C7</accession>
    <name>A0A1E3P6C7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Thiamine transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_92995</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039709.1">
        <property type="nucleotide sequence ID" value="XM_019186391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6C7"/>
    <dbReference type="GeneID" id="30203637"/>
    <dbReference type="OrthoDB" id="2018619at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF15">
        <property type="entry name" value="NICOTINAMIDE RIBOSIDE TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="480" mass="52986" checksum="ED5C4D46AE47E3A5" modified="2017-01-18" version="1">MAALTIGNILIVLYTILNSYPGQVYHIGFTVCQRIVFGVYGSGLGILIRIILSITYFGSQAWLAGLCVTVMLSSITPNYLNMENTFPESVKMSTRDFVSFFIVQIISIPFFLIKPEKINIFVNASCVMALAGMVGMFGYMVKLNGGPGSIYYEKVTLSNSEFGWMYLMGINVWYGALSPDICNQSDFSRFSSSKPKLQWGIFCGIFFTEIIPLAGLICASISTDLYGKQYWLPTDICLQWLNDHYTPRTRCACFFLGLSFLTSELSLNTLTNGFAGGMDLAGLCPKYINITRGSIITALLSWVVQPWLFYNTSSTFLGVMSSFGVVVTPIIAIIVSDFMLVRGGKLKLNDLYTTSKDGSFYHTAGVNWRAVFVFFASVVPGLPGMAAYVNPSIKLKTSIINYFYGSTDRKIAAGIDLYNAFTLDEYKRLNLIPYDGVNPEEVIVLEDFTKDDTHGGNSTSDSTSGIASSKEKIKSVFNVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6J5</accession>
    <name>A0A1E3P6J5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sphingolipid long chain base-responsive protein LSP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_29261</name>
        <name evidence="5" type="ORF">WICANDRAFT_29490</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
            <strain evidence="5">NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040165.1">
        <property type="nucleotide sequence ID" value="XM_019181590.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040256.1">
        <property type="nucleotide sequence ID" value="XM_019181615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6J5"/>
    <dbReference type="STRING" id="683960.A0A1E3P6J5"/>
    <dbReference type="GeneID" id="30198836"/>
    <dbReference type="GeneID" id="30198861"/>
    <dbReference type="OrthoDB" id="5599269at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036286">
        <property type="term" value="C:eisosome filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000005">
        <property type="entry name" value="Sphingolipid long chain base-responsive pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028245">
        <property type="entry name" value="PIL1/LSP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962:SF1">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13805">
        <property type="entry name" value="Pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="277"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="280"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="302"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="312" mass="35040" checksum="BB2BA8D1F74AE7F2" modified="2017-01-18" version="1">MHRTYSLRNSRAPTASQLQSPPPPTSTTKSKFFGKAGIASTFRKNAAGNFGPELARKLSALIKLEKNVLRSIELVARERREVAKQLSIWGEDNDDDVSDVTDKLGVLIYEIGELEDQFIDKYDQYRITLKSIRNIEASVQPSRDRKQKITDEIAHLKYKDPQSPKINVLEQELVRAEAESLVAEAQLSNITREKLKIAFNYQFDAVRELAEKFALIAGYGKALLELLDDAPVTPGETRPAYDGYEASKQIIIDAENALASWTLDQAVVKPALSLHHTSEDVYEPAEEEAAEAEWAEEHEQAETEPVEETTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6Q5</accession>
    <name>A0A1E3P6Q5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_61670</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040308.1">
        <property type="nucleotide sequence ID" value="XM_019183228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6Q5"/>
    <dbReference type="STRING" id="683960.A0A1E3P6Q5"/>
    <dbReference type="GeneID" id="30200474"/>
    <dbReference type="OrthoDB" id="2595934at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR OF PROTEASES PRTT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="18"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ61101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="573" mass="65160" checksum="B28EBDA546212E4B" modified="2017-01-18" version="1">MTEPTQIKHVHVPRAAKACLACRRQKTRCFPSNDSNSCLRCQSLSRPCSFLKDGGADSSSGGGGNASTGSSGNSNRKRSISAIDTSTTIDGVNIIELDKNVKKILTLLQPKTHPITSLNRGEDDSTTTKHKSPFQTSPFTLISNSVSSQSSTIRQLFNPTNIKPTPPISVLTLEIITIKEAITLVDKFKECYGRWISLPNNITTEELINSIQISSPILLTSICVTSLRYQKQTNHDNKLYLDLIKQLKLELFQEISNPINSLEFLKALVILSVYGYSLSVSDFTIDPWFLSGIAVQKFLTLDVDSELLNLDNLHESLDLEQECEKLSNFRIWNHICLVHLVNCVFSSRMCVLDEIRLDQSRKSLDLSQATNFDGRIVSEISLQLLLYNYLQSGSTTNDLGNHFNTDFESFEQELNLWHEQWNYLFRQPTLQFVEFGYHYVYLVAMYHYQFGGDKKIDELDQEDEIKDKEVLKKFIYHSIRLIDHINGIKDIVYFLCLSDQIHLCTMYTTLLFIKLIKWSYKYAGLGQIDMLDLISKAKLIKEKFIQVSRETNDLAFTFATSIQEAIDSVLAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6Y0</accession>
    <name>A0A1E3P6Y0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">S16a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_83350</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. eS19 is required for proper maturation of the small (40S) ribosomal subunit. Binds to 40S pre-ribosomal particles, probably required after association of NOC4 but before association of ENP1, TSR1 and RIO2 with 20/21S pre-rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040330.1">
        <property type="nucleotide sequence ID" value="XM_019185526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6Y0"/>
    <dbReference type="STRING" id="683960.A0A1E3P6Y0"/>
    <dbReference type="GeneID" id="30202772"/>
    <dbReference type="OrthoDB" id="428974at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000118">
        <property type="entry name" value="40S ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_eS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018277">
        <property type="entry name" value="Ribosomal_eS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00628">
        <property type="entry name" value="RIBOSOMAL_S19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00055140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00075881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="144" mass="15714" checksum="700CAE1D185ABD4D" modified="2017-01-18" version="1">MPGVSVRDVPAQDFINAYASFLQRQGKLEVPGYVDLVKTSAGNELPPQEAETWFYKRAASAARHIYLRKQVGVGKLNKLYGGAVNRGGAPARHVDASGSVNRRVLQALEKIGVLEISPKGGRRISDNGQRDLDRIAAQTLEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6Z3</accession>
    <name>A0A1E3P6Z3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_77651</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040201.1">
        <property type="nucleotide sequence ID" value="XM_019184591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6Z3"/>
    <dbReference type="GeneID" id="30201837"/>
    <dbReference type="OrthoDB" id="3982796at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="261" mass="30962" checksum="9EAB8A61EBB139B2" modified="2017-01-18" version="1">MSPRQREFILKVNKELFLEFGEVPEKRKEYLKLKRLLDKKHRHGREFNDHQELLEVFKSITEDQSIKRNTQIIAKIDMGEMSFILTQDLFQYVQIGDDLYDLNSRFYEFSRRIYDYCLVQSFLDYTPELGLFMKILDFKMQRLFATEKYKSPSQRKDTFLVHMAMDKAAGINAPLVKATEKFMKLYPKEVTRFISSSPNKDRNPQDFTDIPDSRCVRRRMKGVPFTNNGARKYLLKDARELLESEDEPPAKHESDVKADSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P717</accession>
    <name>A0A1E3P717_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase RBP11-like dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_104276</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040370.1">
        <property type="nucleotide sequence ID" value="XM_019180409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P717"/>
    <dbReference type="STRING" id="683960.A0A1E3P717"/>
    <dbReference type="GeneID" id="30197655"/>
    <dbReference type="OrthoDB" id="10248581at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06926">
        <property type="entry name" value="RNAP_II_RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.10:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037685">
        <property type="entry name" value="RBP11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008193">
        <property type="entry name" value="RNA_pol_Rpb11_13-16kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I,II,III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946:SF16">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01154">
        <property type="entry name" value="RNA_POL_L_13KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RBP11-like dimerisation" evidence="6">
        <location>
            <begin position="31"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="122" mass="13976" checksum="EC18BEE2A076C642" modified="2017-01-18" version="1">MNAPDRFELFILPDGVSKIKVEPDARVPNAAIVTFEKEDHTLGNLLRAQLLKDERVLFAAYKVEHPLFAKFVMRIQTEENYSPREALRNACNSVISQLDVLRERFKNEWALKSFAADAADLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P746</accession>
    <name>A0A1E3P746_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NADH-cytochrome b5 reductase</fullName>
            <ecNumber evidence="12">1.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_90595</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46680"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11 12">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="12"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040456.1">
        <property type="nucleotide sequence ID" value="XM_019186247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P746"/>
    <dbReference type="STRING" id="683960.A0A1E3P746"/>
    <dbReference type="GeneID" id="30203493"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090524">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NADH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000009">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF143">
        <property type="entry name" value="PLASMA MEMBRANE-ASSOCIATED COENZYME Q6 REDUCTASE PGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 12" id="KW-0274">FAD</keyword>
    <keyword evidence="4 11 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0520">NAD</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="14">
        <location>
            <begin position="66"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
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            <position position="131"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
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        <location>
            <position position="133"/>
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        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
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        <location>
            <position position="139"/>
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        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
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        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="141"/>
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        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
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    <feature type="binding site" evidence="11">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
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        <source>
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    </evidence>
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    </evidence>
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        </source>
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        </source>
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        </source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ61249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="304" mass="34501" checksum="9F0B147B1E7D83D8" modified="2017-01-18" version="1">MSSKVEDKTELLKQPLHGIYIPVILVIVGICIMNVDYLPHVLVLLVSLLGFRLFNAYNRRRSLDEKKWRSLELLDKTIISRNTAIYRFGLVREDEVLEIPVGHHVAVKVEDEIRHYTPISSKFDKGFFDILVKSYPDGKVSKKFAEMKPGQFVDFRGPVGRFSYSTNQVKEIGLVAGGSGITPILQVISEITTTPEDFTKLSLIYANETENDILLREEIEELNAKYPNIDIHLTLTHPPANWEGETGYVTKEMVQKYLPKPSEDSRILVCGPQGMKTLLIDITKELGFTEAKMPSKGNDQVFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P786</accession>
    <name>A0A1E3P786_WICAA</name>
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        <recommendedName>
            <fullName evidence="11">DUF788-domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="9" type="ORF">WICANDRAFT_77886</name>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
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        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <text evidence="2">Belongs to the TMEM208 family.</text>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="PANTHER" id="PTHR13505">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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            <end position="38"/>
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    </evidence>
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    </evidence>
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            <dbReference type="EMBL" id="ODQ61243.1"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="179" mass="20905" checksum="6F080F1A64FE2F53" modified="2017-01-18" version="1">MAGQSAKKQVAQNFKILKEIHLITWISYSIFTFFNYFLNRPQSSKFFIITAVPVFGSLYVIEKFGRPVLDPQGKIIRQGQDLQQEGLTEYLFDIIYYTVILNFLTILFNSSKIWWLYWAIPVFAVYKIYGVINAGRQMFGGGAKSGQQVEEYDGEKEQAKSKRQQKREARGDKPKMRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P791</accession>
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        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
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    <organism evidence="2 3">
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Phaffomycetales</taxon>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="GeneID" id="30200504"/>
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    </dbReference>
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    </dbReference>
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    <dbReference type="Pfam" id="PF00646">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="7"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="254" mass="29818" checksum="D7D316BB6F5808A3" modified="2017-01-18" version="1">MDIETINFPLRQLPPEIFQIVLFHLSQASIISLSKTCRALKQQCYSKLYGNIYIIVSTYCRSYEFTVTKKMKSRMTLSQNFTVISGLLSLSKFVTHTLPTLRTFVTKLYVDFLSFDKVLNPRIFDLLFDGPHAGDPLDVRDFEVPNDKYLIMEDINSVRKLFLRHFFLRLRKMDGYEVEYFKKIDNPLSFSGDLISYSTVINYEAMIIDLNEVIDGCERALVLDYRTYEAWPLLQHKVTQKTIHVHRAHYIARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P798</accession>
    <name>A0A1E3P798_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Swiss Army Knife RNA repair protein HAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_67771</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019184099.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="30201345"/>
    <dbReference type="OrthoDB" id="5596992at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990259">
        <property type="term" value="F:histone H2AQ104 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018812">
        <property type="entry name" value="SAK_HAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335:SF23">
        <property type="entry name" value="OB FOLD-CONTAINING PROTEIN, NUCLEIC ACID BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Swiss Army Knife RNA repair protein HAD" evidence="2">
        <location>
            <begin position="128"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="496" mass="56807" checksum="DF6A0F2C7E1AA01F" modified="2017-01-18" version="1">MDQNTTDQTALSFPVGVPPFFFMPPIPPIGQMNQLTPFPKADVASIEQGASKDEPSQKMRKKNKAKEKKKNKNHPNKGPASTKSTDLHVGKENPLEFWDCSNDLGDIPSVEQIKKIHIYDFDNTLFSSPCPNPNLFNEQTIGMLTNSDMLHYGGWWAEPLIFKNAGEGWDVESKRQWESFWNGDVEDLLRLSYEQDDALAIIMTGRKSHLFTDLFKEILDIRGIKFNGLMLKKGNFPSTITYKTQVLTDILDYYENIEKVTIYDDRPSQLKGFQKCLNEYNEAVRPELIYNLVPVAPEVKYLEPKTERKIIEEIVEQHNEFVDQGKSTGKLGKVSLKQSFFYSGYMLEVESKAKVLEYILDKYDTAFTPEFQKKLKFLLGFIPIAKSKVSKALELELSSEPVIEWKITEFGGLNDEYFGLSVVPINSNVQVKTLFDPPFLSFATTKKSTVSGLEEFEKITDWEPIEDDIRIKTHFGQVVQFKIVTDNTRKRKRPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8C4</accession>
    <name>A0A1E3P8C4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-classical export protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_59745</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019182866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8C4"/>
    <dbReference type="STRING" id="683960.A0A1E3P8C4"/>
    <dbReference type="GeneID" id="30200112"/>
    <dbReference type="OrthoDB" id="2155101at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024242">
        <property type="entry name" value="NCE101"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28011">
        <property type="entry name" value="NON-CLASSICAL EXPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28011:SF1">
        <property type="entry name" value="NON-CLASSICAL EXPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11654">
        <property type="entry name" value="NCE101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="54" mass="6128" checksum="B3D50E4B1C344842" modified="2017-01-18" version="1">MASYPYLISRTLDPLFGVFIGVASFYAHEQRVGRKPGHSLNELVAKRFSSEKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8C6</accession>
    <name>A0A1E3P8C6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_66025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040872.1">
        <property type="nucleotide sequence ID" value="XM_019184028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8C6"/>
    <dbReference type="GeneID" id="30201274"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="165" mass="19299" checksum="8005612E65A674D9" modified="2017-01-18" version="1">MDRIRLNIQTITNNLQQHINPNFNFNFNNFIINNNHHAQYTAGRNNIKSINTGFLSFDFDRLGIHDGSNQLIGSSIGDYTKLLVPLMQNLPKISQPFLNVDLIEQFSNRDNCWMSKSKCCYVSHEFLMNVLFLSYGVEKNQKQKICYIIKGEDPMYTYYWEITPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8H4</accession>
    <name>A0A1E3P8H4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_25483</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040916.1">
        <property type="nucleotide sequence ID" value="XM_019181152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8H4"/>
    <dbReference type="STRING" id="683960.A0A1E3P8H4"/>
    <dbReference type="GeneID" id="30198398"/>
    <dbReference type="OrthoDB" id="672793at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043530">
        <property type="term" value="F:adenosine 5'-monophosphoramidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01277">
        <property type="entry name" value="HINT_subgroup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039384">
        <property type="entry name" value="HINT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648:SF1">
        <property type="entry name" value="ADENOSINE 5'-MONOPHOSPHORAMIDASE HNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648">
        <property type="entry name" value="HIT FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="4">
        <location>
            <begin position="6"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="2 3">
        <location>
            <begin position="101"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="1">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="146" mass="16021" checksum="D82EF765B1DE6219" modified="2017-01-18" version="1">MSAPCIFCKIIKGEIPSFKLFETELTYSFLDIQPTAKGHALVIPKEHGGKLHNLSDESLADLLPMAKKIAKLLDVTNDGPEGVGYNILQNNGRIAHQEVDHVHFHVIPKVDKETGLGVGWPAQATDFEALGKLHKELLAKLESGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8M2</accession>
    <name>A0A1E3P8M2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">[acyl-carrier-protein] S-malonyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_27245</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>holo-[ACP] + malonyl-CoA = malonyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.1.39" evidence="1"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040970.1">
        <property type="nucleotide sequence ID" value="XM_019181353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8M2"/>
    <dbReference type="STRING" id="683960.A0A1E3P8M2"/>
    <dbReference type="GeneID" id="30198599"/>
    <dbReference type="OrthoDB" id="541883at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.250">
        <property type="entry name" value="Malonyl-CoA ACP transacylase, ACP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050858">
        <property type="entry name" value="Mal-CoA-ACP_Trans/PKS_FabD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42681">
        <property type="entry name" value="MALONYL-COA-ACYL CARRIER PROTEIN TRANSACYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42681:SF1">
        <property type="entry name" value="MALONYL-COA-ACYL CARRIER PROTEIN TRANSACYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Malonyl-CoA:ACP transacylase (MAT)" evidence="5">
        <location>
            <begin position="4"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="314" mass="35558" checksum="908F746D9CFB75CD" modified="2017-01-18" version="1" fragment="single">EYNLKSYKQSLQSQISPEFASNVFNPAADFYEKTSNTQPSIISISLLILDILKRHHDIDLISKGGIDYVMGHSLGEFAALVVNDVFKHHPDEILWLVRQRGLLMEQALSHSPDKFKMSALVFPAMNFDDVEVLLKDSLKEVGTVGVANINNPQQIVISGEEKEVDQVVQLIKDKSNIKRRLRVFPLQVTTPFHNPLLESSKIKFNHIIEEKFNIERDQRLKIPILSNLTGKPVYTHGEALDNFIEDFINPVQFKKSIEYVIDESNDDLHFINIGPNASINQNSVTKTDVSKKVSSNTPVGTVEEIKAFVEAYEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8Y2</accession>
    <name>A0A1E3P8Y2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">assimilatory sulfite reductase (NADPH)</fullName>
            <ecNumber evidence="4">1.8.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_98522</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hydrogen sulfide + 3 NADP(+) + 3 H2O = sulfite + 3 NADPH + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.2" evidence="4"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041076.1">
        <property type="nucleotide sequence ID" value="XM_019186656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8Y2"/>
    <dbReference type="STRING" id="683960.A0A1E3P8Y2"/>
    <dbReference type="GeneID" id="30203902"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009337">
        <property type="term" value="C:sulfite reductase complex (NADPH)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004783">
        <property type="term" value="F:sulfite reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06207">
        <property type="entry name" value="CyPoR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000010">
        <property type="entry name" value="Sulfite reductase [NADPH] flavoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000011">
        <property type="entry name" value="Sulfite reductase flavoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF109">
        <property type="entry name" value="SULFITE REDUCTASE [NADPH] FLAVOPROTEIN COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0288">FMN</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="15">
        <location>
            <begin position="713"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="665"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="666"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ61869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1100" mass="121677" checksum="7D202AF17795A1F7" modified="2017-01-18" version="1">MACAALATVNSREIVYREEIILEIVANAKKPYQSSSVVNLSAASISSPFGLTKDPQSLKGTVYTTPNGLINSAIYANASKIFSYKSIGHENALDIGLKLWSVLQRKNPNGVVPNLSSFEVRSGASNAVLGYLSAASQGGKDSNGEVYPVVASGSALPYFQGQLSKFTNEKLPLSFQIAALDYDSEANALVPNYVTPLSVANALNYTVFTPLSASEAQPLALLSSVFANVSPAVNLYDGPTYLKESSRIDNVLDFETLSSTYNKLKTAVSGWESLPFVKGPSAALSALNSILGTDYKPFEYYGHKDATSVFVVYGSVESELFAKEISELALKSHLKIGVIAVRVPLPFDAEEFVSVIPESTQSLIVIGQSLDNKSSLLKTNVASTIFFNGLAKQVKVSEFIYSPSFIWSSIAVEQIIGSFIVLPKKQPNVPANLGSSVPYGDFIFWSLDNDKFVDVASKIAHAFSLDQGSIVKFRSKFDNLIAGGVYQAQISSTKSDFKLESPTVGAVDRADLVVVETLEILKNLNVVKTIKQKGTIVLVHQDLPKDLQSFVDSLPVQFRRNVAKNENKVVIVDLATIGDYAQTQGRTGLIAIQSIFWKYSAPQLNINDIVRRVLHASGSDIELLPAVLVSIITDIFEKGIKEVQFDPKWAELPIEENEVTLPIFPTESSFEPNPRSTPEPEQPEETSYVEIAKKFAFGEAYGIDNNLRPDLPVRNFVVKVQENRRVTPNDYSRNIFHIEFDITGTGLTYDIGEALGIHGRNDPEEVLRFLEFYGLNPEELVQVPNRDNSDIVEVRTVYQAFTENLDLLGKPPKRFYESLAAFATDENEKKKLELVASAEGAPELKKYQDEEFYSFSDILELFPSAKPSLTELLSIIPPLKRREYSIASSQKMHPNAVHLLIVVVDWVDNRKRKRFGQCSRYLSQLSIGSELVVSVKPSVMKLPPLSTQPIVMSGLGTGLAPFKAFVEEKIWQKQQGLEIGEVYLYLGARHRKEEYLYGELWEAYKAAGVITHIGAAFSRDQPEKIYIQDRIRQTLPELKEAIIKKNGSFYLCGPTWPVPDITAALEDIVAEEAKERGVEVDAAREVEELKETSRYILEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P952</accession>
    <name>A0A1E3P952_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">30 kDa heat shock protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_78108</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal/bacterial/fungal opsin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040689.1">
        <property type="nucleotide sequence ID" value="XM_019184661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P952"/>
    <dbReference type="GeneID" id="30201907"/>
    <dbReference type="OrthoDB" id="536545at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd15239">
        <property type="entry name" value="7tm_YRO2_fungal-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001425">
        <property type="entry name" value="Arc/bac/fun_rhodopsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043476">
        <property type="entry name" value="Yro2-like_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28286">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28286:SF1">
        <property type="entry name" value="30 KDA HEAT SHOCK PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01036">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01021">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="274"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="34650" checksum="AAF42B25F037A446" modified="2017-01-18" version="1">MAGNEATLINKSSAGISITDHGSDWYWTCFSVFGLFALLHASIFIVFHKSSTQERRNKAMFHVGTLSSNAVLAFTYYTLASNLGYAPVAVEFNHIQIGDDTRQVFYARYIGWFLAYPGLLYTFELNSLAQNILSRTDYLQLFQTLFLQICSTEVLVLGLLIGSVVHSSYKWGYWTFGVASQLFMITIFFYHQFKTASTTSLITKLLVPFFSLCFILYPVCWGLSEGGNVIQPDSEGVFYGVLDLINFWLIPLALIFDSYKHGTVINEAHLPTRGDDVEKQTSPELRASGETEVGNQENPDEGALETQHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9C8</accession>
    <name>A0A1E3P9C8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UNC-45/Cro1/She4 central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_59900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041031.1">
        <property type="nucleotide sequence ID" value="XM_019182899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9C8"/>
    <dbReference type="STRING" id="683960.A0A1E3P9C8"/>
    <dbReference type="GeneID" id="30200145"/>
    <dbReference type="OrthoDB" id="4059796at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040144">
        <property type="entry name" value="RAP1GDS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10957">
        <property type="entry name" value="RAP1 GTPASE-GDP DISSOCIATION STIMULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="558" mass="62944" checksum="9829F8736444DE88" modified="2017-01-18" version="1">MAEMDLEHVLFGLSTFVNDCEEKKLNVDNVYKESYLKLLDQMALLLREESTRDHDLIRSNLPKFIDSADYLLVNQEQPGYNELLLEVIRVLANSIADNDVNRRQLSSKSDFIEHLSNQLESKPDDSSLNERILILLKNLVIDTPDVAKDVSFITQSLLSYISQSDGGIFMAVDLLTDLIGNMQYNPNPQNIENLIKLLVKTLNQQSDLDEDEFAELVVNISSVLEGITVDPRFKFDKAETEKTLQVGLFDSLKKIDSLEFQNILMAKRRLFASIGNISANLTTSNKPMRTYSISNVQDQNQQNGYIISGCFTIIGNSIASPEDRNEVLDQAPALVNDILSKYNYLTDPVQFQGILHLLKALTNFDTVGTLFTDKNFKVLEFLIEATVRNSKYYTNFNVLLTGFLKKLFVLVNQRHVEKILNSGVPEILTSSDPSNESNLIILLLLNKVAIYKSDSNIDIFCNRIFKFSNDIQAAYLFELTKTIGVLLQHRSSYVLENYSSSLITLLTTIDSIDASSNDNAAKAVLNNGRYISGTLLNLSKENDIDPELKALSEKLLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9H8</accession>
    <name>A0A1E3P9H8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Lipoyl-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_22294</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Eukaryotic pyruvate dehydrogenase (PDH) complexes are organized as a core consisting of the oligomeric dihydrolipoamide acetyl-transferase (E2), around which are arranged multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3) and protein X (E3BP) bound by non-covalent bonds.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ61612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040819.1">
        <property type="nucleotide sequence ID" value="XM_019180936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9H8"/>
    <dbReference type="STRING" id="683960.A0A1E3P9H8"/>
    <dbReference type="GeneID" id="30198182"/>
    <dbReference type="OrthoDB" id="202158at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004742">
        <property type="term" value="F:dihydrolipoyllysine-residue acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000010">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="entry name" value="E3-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045257">
        <property type="entry name" value="E2/Pdx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151">
        <property type="entry name" value="DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151:SF82">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE COMPLEX PROTEIN X COMPONENT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="5">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="6">
        <location>
            <begin position="97"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00065810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="14664" checksum="BB295083BB8BA62F" modified="2017-01-18" version="1" fragment="single">SMPAMSPTMNEGGVTEWKFKEGDSFKSGDILLEVETDKSQIDVEAQDDGILAKIIKADGSKDVPVGEAIAIIAEPGDDLATLELPEIKSMKADPKQTFFPSVASLLEANKISKEDALSNIKASGPNGRIVKGDVLAYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAC4</accession>
    <name>A0A1E3PAC4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_82405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041560.1">
        <property type="nucleotide sequence ID" value="XM_019185374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAC4"/>
    <dbReference type="STRING" id="683960.A0A1E3PAC4"/>
    <dbReference type="GeneID" id="30202620"/>
    <dbReference type="OrthoDB" id="1112565at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="547"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="574"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="616" mass="68808" checksum="B69B65E62C24067A" modified="2017-01-18" version="1">MSENSAQQSKKPAFFRTLPENIATSPIGIVKISNSPFPSLDPTRQFRSVYERAGFDVYRPNKTRQSSRVTSPVIKENGFRSQSTSQLNQQTGMPKVFDEKRNFSEHHNISHSNHNYGDPIPGFNKAPLTIDANLANGSKDGEITTHLSTGSTATSTFDRAGPKPLHSSTSSTFTDTEPRSASTETFSKFREGSSSNFVSAQSLPYSARSKRSSKDSTHTYNTSPSSSRIARESNGYHSPAQSFPRISPTRKLFAPQNYANEPEVQQGRGYMAHESNDLSFQASTGGDRSIDTVKYTPEERLEPSLDVSMNNVPRGFPDKSYESVNPLSNSTSFDVSEPIDVISDDGVDNSDDESFKSEILSPKNQHFNFENGKDQQLDDSLSFGQDSTQRDISLQLPVSPPQSQQQEQLNTIPQLTVSDTEGNYVHHDDFLPPSDYKKSTDMRQSAVSIAASSIYSNDNRQSITPISEFETIEEHEFENYPDLPKSSGPVYEEIEQQLESLSMKSPQPSQPPQILIHSLKEVSTDAFDEEHDEPEDVQEFQKTNGARSIDNVSKNNEYTTQDQDEYVSKSPNLIEEHKQEPEVPKYPAGEENQYILKVVNYQVNGTENALHVLYVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAE6</accession>
    <name>A0A1E3PAE6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_76450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041477.1">
        <property type="nucleotide sequence ID" value="XM_019184387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAE6"/>
    <dbReference type="GeneID" id="30201633"/>
    <dbReference type="OrthoDB" id="10645769at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="249" mass="28330" checksum="A4DECA698692325A" modified="2017-01-18" version="1">MHRYNESVLHKWTNNAERELYLNGNNHDLNDSRLFDYNEELNNAVEESWIDLEKFNASRRQDRVNEGEEDEEDDDDDSRTLRLNSEESGNEDEYVKNPNRRINFELNDAMGTDIKSKRSFCDGYDLNTSLTTILSREKQHSTENSKIMFMDVSNSSSMLSNVSGSELSFKASTKKRKIKSSTSQSNSGDYANSIVFISIIASLSFSAGYALGQKSEEVIIVKPITKLKPRSARFNGQDSGTNWESATRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAT1</accession>
    <name>A0A1E3PAT1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_82540</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041732.1">
        <property type="nucleotide sequence ID" value="XM_019185395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAT1"/>
    <dbReference type="STRING" id="683960.A0A1E3PAT1"/>
    <dbReference type="GeneID" id="30202641"/>
    <dbReference type="OrthoDB" id="3969558at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000211">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="57"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="510" mass="55905" checksum="73F0F84ABB452FBD" modified="2017-01-18" version="1">MTSTLEDKFFDLCTVYTIFFLVKKWLFMALIFVHPSTLLSSYAAESQLIVSTLGKEKSHVNVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYHVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGIGEFEAGISKDGQTREHALLAYTLGVKQLIVAINKMDSVKWDESRFEEIVKETSNFIKKVGYNPKTVPFVPISGWNGDNMIEPSTNCPWYKGWKKETKAGEAKGKTLLEAIDAIDPPSRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMVVTFAPAGVTTEVKSVEMHHEQLTEGLPGDNVGFNVKNVSVKEIRRGNVCGDSKNDPPKAAESFNAQVIVLNHPGQISAGYSPVLDCHTAHIACKFDTLIEKIDRRTGKKLEEAPKFIKSGDAAIVKFVPSKPLSVEAFTDYPPLGRFAVRDMRQTVAVGVIKAVEKSDKAGKVTKAAVKATKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB02</accession>
    <name>A0A1E3PB02_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_27368</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041677.1">
        <property type="nucleotide sequence ID" value="XM_019181366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB02"/>
    <dbReference type="STRING" id="683960.A0A1E3PB02"/>
    <dbReference type="GeneID" id="30198612"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04098">
        <property type="entry name" value="eEF2_C_snRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01683">
        <property type="entry name" value="EF2_IV_snRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000009">
        <property type="entry name" value="116 kDa U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000646">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000022">
        <property type="entry name" value="U5 snRNP-specific protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1430.10">
        <property type="entry name" value="Yeast translation eEF2 (G' domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031950">
        <property type="entry name" value="EFTUD2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035655">
        <property type="entry name" value="U5-116kDa_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF6">
        <property type="entry name" value="116 KDA U5 SMALL NUCLEAR RIBONUCLEOPROTEIN COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16004">
        <property type="entry name" value="EFTUD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="8">
        <location>
            <begin position="82"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="916" mass="102651" checksum="5170154F9126C3C0" modified="2017-01-18" version="1">MDSDDEQNNNQVILQEDKVYYSTMSETFGNDVETVIQTTDNQTIDQPLVNPQIEKKFKIEDKNLPKTTYSKQYLAETLNYPNKVRNVSLVGSLKSGKTSFLDTMILQTHQLKKLSKNQKSFKKLRYTDNETLEVKRGLTIKLSPMTILLPNLRGSSVAINFIDTPGHVNFSDEMSVAQRLTDISVVVVDVVEGLTIGARQAIDNALNNNVELAFVINKIDRLVLELRLPPLDSYHKIRNTIDEINTYIQENQYLGNYKHNSLISPELNNLLFASADLNFSFSLKSFATLYADRYGMDIDAVAFSKRLWGDVYFDEELNKFTTKSSKGLKRSFIHFILEPLYKIISLVLATEPEDLQKSLYESFNVGLDKQLLKSDAQTLLKETFKLIFGGSQGFVDIIEGLKSPEEHSKTNLYTGPESDLSKSILLASSEGPLVAQVGKIIENSTALVRVLSGSIEKGQTIKVLGEHYNDDDEDLQILTVDELFLGCGRYKIPIDKAPAGSIVLVGGIEVISKSGTIVGNDIQEDVFTFRPIDYINKAVFKVVVAPDVPSELPKLLDGLRKINKTYCGVEVKVEESGEHVIFGSGELYLDSLLRDLREMTRINIKISDPITKFSETVVDKSITKVSIKSQNGQNEITIIAEPLEENLATDIENNKFKDVKRINKVLRDNYGWDSLAARSLWTFGPDSNGPNTLLNDTLPDEVDTNLLNSVKKSIIQGFQWAVREGPLADEPIRNVKFKLIDISLSKDSLQRGNGQIIPMVRRACYASILTSTPKIMEPVYSIEIISTAQTVRIIEDILDKRRGAVFKDTPIAATQLYKIQGFVPVIDSIGLETDIRVATQGQALVSLYFEKWTVVPGDPLDQDVFIPKLRPAHINSLARDFVMKTRKRKGLNGEPTLAKYIDKELVDNLKELGLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB42</accession>
    <name>A0A1E3PB42_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">HTH La-type RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_60568</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041717.1">
        <property type="nucleotide sequence ID" value="XM_019183018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB42"/>
    <dbReference type="STRING" id="683960.A0A1E3PB42"/>
    <dbReference type="GeneID" id="30200264"/>
    <dbReference type="OrthoDB" id="439993at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140691">
        <property type="term" value="F:RNA folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042780">
        <property type="term" value="P:tRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061818">
        <property type="term" value="P:tRNA folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08029">
        <property type="entry name" value="LA_like_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12291">
        <property type="entry name" value="RRM1_La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000158">
        <property type="entry name" value="La ribonucleoprotein domain family member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002344">
        <property type="entry name" value="Lupus_La"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF140">
        <property type="entry name" value="ACHILLES, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00302">
        <property type="entry name" value="LUPUSLA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="7">
        <location>
            <begin position="71"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="171"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="257"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="29"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="33229" checksum="201E6B713E626C8C" modified="2017-01-18" version="1">MSEAKEVQAVESVEVPKTEEVTSVEEPVVESKTEVTEEDKKEEQSEAKPEDAKADDAKLEEPKSEETKTEEAASEFEPEEVLKQVEFYFSDSNLPKDKFLWRTVQSNQEGWVSISTIASFNRMKKFRPLSAVVDALKKSEFLVVSENGEMIKRKNPILPPSDSDKQSRFQRSIYAKGFGEETPTTQEDLEAFFQPFGKINEVRLRRDDNKKFKESVFVEFADSEDAKKFLELSEKPKYKDNELLIMSKPAYVEMKANNENFGGNKNNKKRKFNGFKNGKRGGNFKRSRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBU2</accession>
    <name>A0A1E3PBU2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein DML1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein dml1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_60929</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the partitioning of the mitochondrial organelle and mitochondrial DNA (mtDNA) inheritance.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the misato family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042090.1">
        <property type="nucleotide sequence ID" value="XM_019183082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBU2"/>
    <dbReference type="STRING" id="683960.A0A1E3PBU2"/>
    <dbReference type="GeneID" id="30200328"/>
    <dbReference type="OrthoDB" id="271881at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049942">
        <property type="entry name" value="DML1/Misato"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029209">
        <property type="entry name" value="DML1/Misato_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019605">
        <property type="entry name" value="Misato_II_tubulin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION REGULATOR MISATO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391:SF0">
        <property type="entry name" value="PROTEIN MISATO HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10644">
        <property type="entry name" value="Misat_Tub_SegII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14881">
        <property type="entry name" value="Tubulin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Misato Segment II tubulin-like" evidence="8">
        <location>
            <begin position="3"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="DML1/Misato tubulin" evidence="9">
        <location>
            <begin position="117"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="364"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ62883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="483" mass="55257" checksum="1E25DB668C251077" modified="2017-01-18" version="1">MPEVINISLSQRANHLSTHFYNAQESYLDYTKSVSKADNNPNVFFKPTLSRDKSTVNYNPRALIWDLKGGFGALGQFEYFVSEEDEDEWKGKTESIAKDRIPKSAYQKALDNNSRVPQLDTSNTLYWTDYSRVLYEPKAYNELKNWEVDPKNYPKGRLALGQEREFVDYQVGVEEWKNDYTGVSFLEDKYRTSLEECDSLTGLNVVSEVDSAWGGFTSEMLHELRDDYNPKNSLFTWGIFNGKKMEDLSNKEILSRIRTFLELQRSSSLFIPLATPKNLPESLQIDRSSHWQTSALQTLVFEPFQTITSQREHNVNFNTIEDNLTLGSQRRIVSTVKASTGELSFDFASEFFVEAKNLHEFSRSSVHRPAKISSQEDFKDPSQLPGKPTSIEGEKVDKSQTDYSSNLAFGTPDSFPDFVNPSDNVTATLGITTAARKTLLTMKTFVSKFVRGDDRENMIQELETLAEDYEHGWNDSDESDDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBY3</accession>
    <name>A0A1E3PBY3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Spt20-like SEP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_60446</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041594.1">
        <property type="nucleotide sequence ID" value="XM_019182994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBY3"/>
    <dbReference type="STRING" id="683960.A0A1E3PBY3"/>
    <dbReference type="GeneID" id="30200240"/>
    <dbReference type="OrthoDB" id="1932706at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021950">
        <property type="entry name" value="Spt20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046468">
        <property type="entry name" value="Spt20-like_SEP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13526">
        <property type="entry name" value="TRANSCRIPTION FACTOR SPT20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13526:SF8">
        <property type="entry name" value="TRANSCRIPTION FACTOR SPT20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12090">
        <property type="entry name" value="Spt20_SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spt20-like SEP" evidence="2">
        <location>
            <begin position="92"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="362"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="438"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="471"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="490" mass="56070" checksum="F8AC18A9BE104595" modified="2017-01-18" version="1">MSVTSAQEKAKRTYNEMKAGIPVNGIPVNGTRQQYLQQQQQQQHRPSPLAQQQRQAQLNGNAHGLAGVRSPNQRRVYNFAETSEEILKKYENFDPSLEFHIHENHYRFGNQDGIISKNSQLVKDFLEYVAREEIPHAIVEVLRDAGIRFYEGCIILKVYDHRNTIEVESINETTKNKELKKVPRSFKTLLKPTQLSLFYDLLYQTDSALQRFSDHLGVSMEAEILSLTKRKLDLSVPSNPYHIPEPFRPQSEYPKINKETGEIIHNHRLESTDPESKAFKPYKDLHDDLPQQNSEYEQFMLLMNDKANHALGDSGSDPSQFTRLRFVEQWRIKKEKLKQQSMSANLTSRQFSSQNGNLTPAQQHQLQQQQRLMQLQQQRMQGMSPLQQQQHLLNQQKAMAQQQLQQQQQQQPQQAPQQNKDVDEDGKKVKKPKKPTKKAAAAAAAAAATAGKTLPGYSGKTLPGGGEEPPKKKRGSYKKKNKTDANTNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC33</accession>
    <name>A0A1E3PC33_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">U6 snRNA-associated Sm-like protein LSm1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LSM1</name>
        <name evidence="8" type="ORF">WICANDRAFT_26643</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the cytoplasmic LSM1-LSM7 complex which is involved in mRNA degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the heptameric LSM1-LSM7 complex that forms a seven-membered ring structure with a donut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042180.1">
        <property type="nucleotide sequence ID" value="XM_019181284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC33"/>
    <dbReference type="STRING" id="683960.A0A1E3PC33"/>
    <dbReference type="GeneID" id="30198530"/>
    <dbReference type="OrthoDB" id="10263346at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01728">
        <property type="entry name" value="LSm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034104">
        <property type="entry name" value="Lsm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044642">
        <property type="entry name" value="PTHR15588"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588">
        <property type="entry name" value="LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588:SF8">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Sm" evidence="7">
        <location>
            <begin position="36"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="164" mass="18427" checksum="2970EEDB9C242610" modified="2017-01-18" version="1">MSASPTPEVSEKSSKNAVEQLSENVAELYLESFSFTTAAAIIGYVDRKVCVTLTDGRQLFGVLRTFDQYGSIVLQDTVERIYLPDNRFGEDYLGVLLVRGENIVIIGDVDIDKEDEPLSKLTRIPFPEAKAEQKQANEKSTVDSKKKTKKLHKFGLVSDHFYVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCE2</accession>
    <name>A0A1E3PCE2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_99209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042101.1">
        <property type="nucleotide sequence ID" value="XM_019186684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCE2"/>
    <dbReference type="GeneID" id="30203930"/>
    <dbReference type="OrthoDB" id="21418at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029196">
        <property type="entry name" value="HAPSTR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38645">
        <property type="entry name" value="CHROMOSOME 9, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38645:SF1">
        <property type="entry name" value="YALI0F12243P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15251">
        <property type="entry name" value="TAPR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="207" mass="23441" checksum="2ABBA07DFFB1033C" modified="2017-01-18" version="1">MDNISNLSSNLPPTKPISEESIKEINQDLVLEFKNAAKSVASLYKLSLQKNSMLRYKGYLDCIDDLLNLIKNDGDVENWALMKKLELEGKLTGSELKKESDKANMSSSPSKQDLLDPQIPMDYDFTMSHPNQHKFPPTMPMLSVDHSNHRVPKDKRLIKTPGTNNNKESPNTDVSDENNSSEDEANIDDSAAKRKLQSMRTDKRVKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCJ0</accession>
    <name>A0A1E3PCJ0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_82592</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041804.1">
        <property type="nucleotide sequence ID" value="XM_019185405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCJ0"/>
    <dbReference type="STRING" id="683960.A0A1E3PCJ0"/>
    <dbReference type="GeneID" id="30202651"/>
    <dbReference type="OrthoDB" id="2130629at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17476">
        <property type="entry name" value="MFS_Amf1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF14">
        <property type="entry name" value="AMINOTRIAZOLE RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="343"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="372"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="445"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="20"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="500" mass="55037" checksum="570591D2FB81DA5C" modified="2017-01-18" version="1">MITVDPRPPAFKNAAHEISVVLLIMFAQLLNQAGTTQTLPMMNLLEKSFPNITPNDKVWFMAAFPLTSGAFILISGKFGDLYGLKKILLIGISWSLIWTLMTGIAGYSHNIVFFCLCRGFQGIGLAFILPSAIGIAGNLYPNGQRKALVFCFVAACAPTGATLGVVFGALTAQFGHWQWAYYSNAIVLALMGVCVVYCVPKIKPHHSSSKMDWLGAITGVTGLILFNFSFNQAPEVGWSSPYIIVLLIVGVLFLIAFFYIEKRVESPILPSEIMNTHLLLILSVIAFGWASFSIWTYYYWTFLLNLKNWTPLSGAASYGCLLFFGIVASLMVSVLIRKIRASYILFAAMCAFFIGITMLSTTPVHQTYFNMIFAQMVILSFGMDMSFPAASLVLSDTLPKRHQGMASSLVSTMINYSQSISLGFAGTAETKIFAKTHDLLKSYRAAMYVGVGLASISILLAIVLVICEQEEDHGESLELPEIQSYSLEKQRTNKKDIEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCZ9</accession>
    <name>A0A1E3PCZ9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Chromo domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_82756</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042039.1">
        <property type="nucleotide sequence ID" value="XM_019185432.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3PCZ9"/>
    <dbReference type="GeneID" id="30202678"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:nucleolar chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="C:site of double-strand break"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="C:SLIK (SAGA-like) complex"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:chromatin DNA binding"/>
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        <property type="term" value="F:histone binding"/>
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        <property type="term" value="F:histone H3K4me3 reader activity"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="F:nucleosome array spacer activity"/>
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        <property type="term" value="P:double-strand break repair via homologous recombination"/>
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        <property type="term" value="P:nucleosome organization"/>
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        <property type="term" value="P:regulation of chromatin organization"/>
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        <property type="term" value="P:regulation of transcriptional start site selection at RNA polymerase II promoter"/>
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        <property type="term" value="P:sister chromatid cohesion"/>
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        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
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        <property type="entry name" value="DEXHc_CHD1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Chromodomain-helicase-DNA-binding protein 2 isoform 1"/>
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        <property type="entry name" value="Chromodomain-helicase-DNA-binding protein 2 isoform 1"/>
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        <property type="match status" value="2"/>
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    <dbReference type="Gene3D" id="6.10.140.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="Tandem AAA-ATPase domain"/>
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    <dbReference type="InterPro" id="IPR041150">
        <property type="entry name" value="Cdh1_DBD"/>
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    <dbReference type="InterPro" id="IPR056302">
        <property type="entry name" value="CHD1-2/Hrp3_HTH"/>
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    <dbReference type="InterPro" id="IPR025260">
        <property type="entry name" value="CHD1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
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    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
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    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
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    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
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    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
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    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
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    <dbReference type="PANTHER" id="PTHR45623:SF14">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
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        <property type="entry name" value="DEXDc"/>
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        <property type="entry name" value="DUF4208"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CHROMO_2"/>
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        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Chromo" evidence="11">
        <location>
            <begin position="165"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="11">
        <location>
            <begin position="265"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="368"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="677"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="956"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1214"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1039"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
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            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
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            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
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            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ62832.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1414" mass="162718" checksum="7E24927A66C4DB81" modified="2017-01-18" version="1">MAPAEFDEEVLENPELYGLRRSGRAHVKPTSFEESDVSDDDDDEISKPKRKKKQIDTYSEDEEEAQEADDQDGFDDEEDDDDEEFGAPKKSKSKLKKRRKVHKKPKISTQQEEVRFSSRNSKAINYQVDEEDDADLLVSDDDEDNYYYYDTNTETTPVPDNGVDLVIDHQLIDPENEPNAFRDLAQAKKDYKFLIKWTGKSHIHNTWEQYSNLVGLKGIKKVDNYIKQFIILDYQIRHDPLTTREDIEAMDIERDRRLDEIAEYKNVERVIDSERSVNDDGDEELKYFVKWRRLNYDEATWELAETIVQIGPNEVKQFQQRLNSKILPSLSSSYGSQRPRFEKLVVQPKFIKNGELRDFQLTGLNWMAFLWSRNENGILADEMGLGKTVQTVSFLSWLIYARRQNGPHLVVVPLSTIPAWQETFEKWSPDLNCVYYLGNTEARKTIRDYEFYNNKKIKFNVLLTTYEYILKDANELGSVKWQFLAVDEAHRLKNAESSLYESLNAFKVSNRLLITGTPLQNNIKELAALVNFLMPGKFQIDEEIDFESSDNDQETYIRDLQSKLKPFILRRLKKDVEKSLPSKTERILRVELSDLQTQYYKNVLTKNYAALNQGSKGGHVSLLNVMSELKKASNHPYLFDNAEEQVLAKFGTSTNGGFHSREDILRGMIMSSGKMVLLDKLLTRLKKDGHRVLIFSQMVRILDILGDYLSIKGINFQRLDGTVPSAQRRISIDHFNAPDSSDFVFLLSTRAGGLGINLMTADTVIIFDSDWNPQADLQAMARAHRIGQKNHVMVYRFVSKDTVEEEVLERARKKMILEYAIISLGVTDGTNRSKKNEPSTGELSEILKFGAGNMFKASENQKKLEDLNLDDVLNHAEDHITTPDLGESNLGGEEFLKQFEVTDYKADVDWDDIIPAEELAKLKEDEKKRLEEQYIQEQMQMYSRRQAVMKNMQRTNTFEEDEDDSESTKKANRKKKADYNKITEKEIRAIYRSIMKLGDISNKWEDLINEGTLPENKNPDLFQKCYDELVEISKDLVKKEEERRSKVLAKMEKEALEHKEKIDKGEVENKDGKNPMLLWMLKRKERKAILFEYQGVKSLNAELILSRPQDLKLVHDAIASFDDPLHFKFNSTIKPVYGWTSNWTQHDDEMLVVGIDKYGYGSWIQIRDDPFLGLSSKMFLNEVNSKDSKDDKNTKKVPGAVHLGRRVDYLISVLKGGDDDNTGGNTPNPSTPNEPKKRTRKPRAEGSAKPGPKPKGVTGAGSRSRTPDYPKVTKRGKKPESAASTPNPEHPEKKHPKKPAAASAPTKKVDEGYESMDEQLCKDTLSPMRHSLKRLNKGATGLDRKEWAKILKTELINVGDFINKTVKKGDVKDEDKFKKHLWFYTSHYWPAKVKSTQIKSMYQKLKDSSGPEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PDB3</accession>
    <name>A0A1E3PDB3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_103589</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042074.1">
        <property type="nucleotide sequence ID" value="XM_019180384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PDB3"/>
    <dbReference type="STRING" id="683960.A0A1E3PDB3"/>
    <dbReference type="GeneID" id="30197630"/>
    <dbReference type="OrthoDB" id="3981110at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF281">
        <property type="entry name" value="BOX A-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="8">
        <location>
            <begin position="204"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="77"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="138"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="249"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="317"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="147"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="318"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="357" mass="40253" checksum="FED889030B7611E4" modified="2017-01-18" version="1">MDFDNDLSLFINNIYNKPQQQQAPSLPKTSNNTKLAALQHESTTNNSIFKMYNKPEFKSLPNNERVKNLTWRLNSLNEASKTKSAKRRTKSASASSNSKIMKPTPQQPTKKKQNSLPKIDPIGDDFDYVAHIKKTSQEEYYPNLPPSSINNNTNNNFKHNSSNSSTSSTTNSNSSLLTSSLTSLSTTSSSHSTITAQTNLIEPNSKLISCTNCNTNTTPLWRRSSNGDVLCNACGLFYKLHGVIRPVKQKQQQQQQQEPQQYKTPITNPSISSQIPTTISSMSSMDTAMSNDFINMDQLQSMIDLKSLDELEYQDSFNFNNKPSQQPSQQSNQFGNDHLTNFMQNYDQDYDWLKLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU38</accession>
    <name>A0A1E3NU38_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_71449</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454216">
        <property type="protein sequence ID" value="ODQ56689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035896.1">
        <property type="nucleotide sequence ID" value="XM_019184246.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU38"/>
    <dbReference type="STRING" id="683960.A0A1E3NU38"/>
    <dbReference type="GeneID" id="30201492"/>
    <dbReference type="OrthoDB" id="3980503at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019436">
        <property type="entry name" value="Say1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF31">
        <property type="entry name" value="STERYL ACETYL HYDROLASE MUG81-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10340">
        <property type="entry name" value="Say1_Mug180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ56689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="37383" checksum="090E58F6A1C8D3D6" modified="2017-01-18" version="1">MSKSKYTEPDQNIVVALAKGVFELPSTFIKINKDPAYSRHTLYAKWEITLLKTFIKNLHYKQLLFFSPNKSIKSQLKSTKSKHGSGLNNYGKELEGFPNAVWLAEKPGREPEDPVIIFYHGGGYLFQFRSDTHLVYLVNLAKKLPSHSILIVDYPLSSHPAPLNYNLDLWSYLFKEENLQNVITMGDSAGGHLILSSLASLKLTTSSMQLPKLFKHVLISPWIDLSSIAPPGENHDILDHDRSYGWGEIFSHNNEPIDVANADYGGLLDEHNTLVTVGELEMLRAGIEIFANRYNLPILLESQGVHDQITMEKFLGYKEGDIFDSIVDFCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUN9</accession>
    <name>A0A1E3NUN9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosome quality control complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_65727</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NEMF family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036053.1">
        <property type="nucleotide sequence ID" value="XM_019183976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUN9"/>
    <dbReference type="STRING" id="683960.A0A1E3NUN9"/>
    <dbReference type="GeneID" id="30201222"/>
    <dbReference type="OrthoDB" id="207084at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904678">
        <property type="term" value="F:alpha-aminoacyl-tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140708">
        <property type="term" value="P:CAT tailing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043043">
        <property type="term" value="P:peptide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.310.10:FF:000003">
        <property type="entry name" value="Zinc knuckle domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.310.10">
        <property type="entry name" value="ibrinogen binding protein from staphylococcus aureus domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021846">
        <property type="entry name" value="NFACT-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051608">
        <property type="entry name" value="RQC_Subunit_NEMF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239">
        <property type="entry name" value="NUCLEAR EXPORT MEDIATOR FACTOR NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239:SF6">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11923">
        <property type="entry name" value="NFACT-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05833">
        <property type="entry name" value="NFACT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="8">
        <location>
            <begin position="547"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="NFACT protein C-terminal" evidence="9">
        <location>
            <begin position="851"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="438"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="703"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="331"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="500"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="798"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="438"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="703"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="722"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="756"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="767"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00070414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ56846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="975" mass="112337" checksum="50BA2639ABECE481" modified="2017-01-18" version="1">MKQRVTSLDLQILVSELQNEITNYRLQNIYNIATSNRQYLLKFGLPDSKKNLVLDCGFKLHLTEFSRPTPQAPSNFVVKLRKHLKSRRLSSIKQVGIDRVLVLSFSDGMYHLVLEFFSAGNVVLLDHERRIMALQRLVEEKGNNDRYAVGETYNLFDESLFEEDEQVEPKFVPIQHDASTIQQWINDKKVTPSDDKGKKNKVLSIQKVLYQKVPYLSADLISKNLHKHSINPSSSSLEYLERADELDTVLKETESEVQTILHTKPLTGYLVLKHNPLYDASKDEEDTKFLYEQFHPFEPAQLKDDEELVQIQGYNKTLDKFFSTIESSKYALRIQNQENQAKKRLDQARNDKQQQVQRLVDVQAVNSLKGETIIFNADIVEEAKAAVQALLDQSMDWKTIEKLIKIEKGKNNKVASAINLPLNLKENKISLNLLTEAAYEDDEEEESESSSDSDSDSDEDEKPKAKKDNKVKNSINVTISLGLSAFANASEYFTVKKSTVEKQKKVEQSASKALKNIEQRIEKDLKKNLKQENDILRKLRNPYFFEKFNWFISNENYLIISGKDDSQTDLIYHRYVNDDDIYVNADIDGSSHVFIKNPNKGEVSPSTLMQAGILSLATSKAWDNKMVTSPWWLYASDVTKNDIDGTILKAGSFRYLKEKNFLPPSQLVMGFAFLWKIKTEENQKELEQQLEELDNLDINDIQQDDKESNEVESKAPEPVEEVKEEEPETTEDAEEAEEGEDQESDDQEETPELESQFDTMSLASKASKQKKPVRGKKSKMKKIASKYADQDEEERRLRMEALGTLKQQNQREEELKKQQIQKINHLKKVEKKKRQEEITANKYANYKENINYDKIMNELTPTLHKEDEILEAIPVFAPWSALQKYRYRVKIQPGNTKKGKAVQEVLHYLNMRPTDPSESDRNYDWPREHEAIAGLKDTELLPSIYVGKLKVLLPGKSSAAKSTNKFANSRKSKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV23</accession>
    <name>A0A1E3NV23_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Secreted protein CSS2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_81182</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036150.1">
        <property type="nucleotide sequence ID" value="XM_019185189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV23"/>
    <dbReference type="GeneID" id="30202435"/>
    <dbReference type="OrthoDB" id="5059029at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046624">
        <property type="entry name" value="CSS2_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20521">
        <property type="entry name" value="DUF6736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133516" description="Secreted protein CSS2 C-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Secreted protein CSS2 C-terminal" evidence="2">
        <location>
            <begin position="70"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ56943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="216" mass="22871" checksum="4780FB5791C17013" modified="2017-01-18" version="1">MVAITKLAFISASLYSQFILSAPVNQTSIPENGSKLSIDDILGVGSIDLEQFSVNETTGLDYGEIRHDGSSLPKREDAHVCLAKKNLKDCVSGGNDIRSVGDGIATVISSRDGICGNTGIQSVPGVTGDIYMKYQSTGKGCHTTAKPRTISGAIKKWISTQSEGQVCKSQCIKITHGGYWTGYVAFGNNKDAVKDVKCDSSIGRVSRCSKGGVNQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVB8</accession>
    <name>A0A1E3NVB8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tubulin binding cofactor C-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_97540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036241.1">
        <property type="nucleotide sequence ID" value="XM_019186588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVB8"/>
    <dbReference type="GeneID" id="30203834"/>
    <dbReference type="OrthoDB" id="194775at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012945">
        <property type="entry name" value="Tubulin-bd_cofactor_C_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07986">
        <property type="entry name" value="TBCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin binding cofactor C-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="96" mass="10876" checksum="39C0684C891B92A0" modified="2017-01-18" version="1">MQNCKIFVKCHQLRIHDSNNSEIFPTIDSQNAIIEGCSNLIFKNEDIQVNDFDSPGSVSSNYTKSGIEQKDQDLYKQLQSADKLQDLLANITAFLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVU3</accession>
    <name>A0A1E3NVU3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">WSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_65492</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036441.1">
        <property type="nucleotide sequence ID" value="XM_019183919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVU3"/>
    <dbReference type="STRING" id="683960.A0A1E3NVU3"/>
    <dbReference type="GeneID" id="30201165"/>
    <dbReference type="OrthoDB" id="5985073at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051836">
        <property type="entry name" value="Kremen_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269">
        <property type="entry name" value="KREMEN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269:SF16">
        <property type="entry name" value="PROTEIN SLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="9">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="88"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="164"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="94"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="164"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="311" mass="31987" checksum="E79DC1DAFE955940" modified="2017-01-18" version="1">MSQVGCYSSLPSSYSDKSTYQYQSSSHCSDECSGYSYFALQGGNTCICGNDAPSGSKSSDCNTPCFGYGEEMCGGSNAYMIFSTGNGGGGSGNSGSSSNGQSSTSTSNTGTSSTTGSNTQTSSSTTSSNTQTSKTSTTSSKETQVTQSVSGSTVVVMSTVAVSATPTSSASESQTQSTSSSSTSPSSSSSSASGSSKPSKKTNLIGPVVGGVVGGVGLIALVSVLFFFLRRRSSKSRDAELADSAYYDAIKRDNSTVARPLSNPFLSKEEFTDQRLNPVMLGRRRISEGSLADEADYSRKILRVANPDDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVV7</accession>
    <name>A0A1E3NVV7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_36311</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036473.1">
        <property type="nucleotide sequence ID" value="XM_019182420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVV7"/>
    <dbReference type="STRING" id="683960.A0A1E3NVV7"/>
    <dbReference type="GeneID" id="30199666"/>
    <dbReference type="OrthoDB" id="10256055at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41677:SF1">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41677">
        <property type="entry name" value="YALI0B19030P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="388" mass="44487" checksum="24CD23BBDFE2709C" modified="2017-01-18" version="1">MAIAVLPTSLTPDVLHDNHSYDVPTDIYEDHVAPLLKELNKFGIDDPSSIQFDPSKHLLFTDDYYEKTKRLTLEELGVTETHQPPISKIGVSEAFPLFTDETIAIMRWEMFQKECFSKCGRIANHSNTGDVDMYLRGFAKEYAPFTFQAWTHPKVMEILSTMAGVELEHMFDYEIGHSNVSIRQPASKILDTSAKYENPDDIPAIVSWHYDSPQFVCVLMMSETDKMIGGETSLLTGDGTVAKVENPKKGWVNVLQGRILRHVAPKPKGDYTERITQVCSFRPRDPLLDNCVLTTIKPATLSGSRYNEFYKQWMNYRLDTLTQRINILKNKINTEIDKGELFNQIETINFIQDKIVEYAEHTWQEFEVVDDGIVQKPASFKVTQSRWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVX6</accession>
    <name>A0A1E3NVX6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_65578</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036528.1">
        <property type="nucleotide sequence ID" value="XM_019183940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVX6"/>
    <dbReference type="STRING" id="683960.A0A1E3NVX6"/>
    <dbReference type="GeneID" id="30201186"/>
    <dbReference type="OrthoDB" id="436519at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050817">
        <property type="entry name" value="DjlA_DnaK_co-chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24074">
        <property type="entry name" value="CO-CHAPERONE PROTEIN DJLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="8"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="87"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="383"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="281"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="385"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="513" mass="60554" checksum="6382C0208AA9F44E" modified="2017-01-18" version="1">MIVQAKEDYYTALEIEVTSHISDVDPSTIKKAFRKLALIYHPDKGKGAGDVIFKNINRAYEVLLDPTKKSEYDKWYKKFYKRTHVTQNHIAKERTKKQKMEQAELRRMKEESGKKNQAEKDAKVQEAERERKAREKQKREEEVLKKRREEEKRKIEEKLRKEKMEQKKKEQERANEEKEQKLRAEQALRDEKARKLREEVLKKQSAERARKEKLKQRSEELKNLFPSNEGKKAKRPASQSAYPNSGWSNPQYYASSGSQQNGRREFARPRKSRFKEQHMSKADFHKKIRELQQEAEKCHEDREKFTNECEDFDKIAERIELSAEDALEKAERKYRESIAIAEAMFNKAKLAQESANMAHFNMELYLKEYEELFNKYGNIYGEPNSEGEEEVSEGSEEEGLEPETPKPFKFDFNPPKTDHFQLKSNPWKTYIDTCKGENKVLCGSVLEKQAQIFIFFQFTSNYPVPSMLLLYCKSKMKLNSTPATHRNTRNKLALETRDLWNKAVQEALKAQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVY5</accession>
    <name>A0A1E3NVY5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_64723</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. MetX family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036576.1">
        <property type="nucleotide sequence ID" value="XM_019183791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVY5"/>
    <dbReference type="STRING" id="683960.A0A1E3NVY5"/>
    <dbReference type="GeneID" id="30201037"/>
    <dbReference type="OrthoDB" id="191364at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004414">
        <property type="term" value="F:homoserine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009092">
        <property type="term" value="P:homoserine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00296">
        <property type="entry name" value="MetX_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01392">
        <property type="entry name" value="homoserO_Ac_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268:SF11">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000443">
        <property type="entry name" value="Homoser_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="5">
        <location>
            <begin position="59"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="236"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="266"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="291"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="388"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000443-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ57369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="471" mass="52355" checksum="0BA837149C3A92A8" modified="2017-01-18" version="1">MGIHKKVESQPENPFSGLVNKQTIVEIPEFVLESGEVLTNVPVAYKTWGQLNKAGDNCMVICHALTGSSDVSDWWGPLLGPGKAFDPTKFFVICLNSLGSPYGSASPVTNDPNTGQRYGPEFPLCTVRDDVRIHKLVLDDLGVKQLAVVIGGSMGGTLVLEWAFYKDYVKNIIPLATSARHSAWCISWSEAQRQCIYSDPKYQDGYYPLNDPPTAGLSAARMTALLTYRSRNSFESRFGRKDPSLVKGIQNKVDKPSINRSPSTPQEEHWAIHNEGHRLGRKLQPNGLEKTGSSSQINSPRPRSESTDSNASLASTASSVHKHQTYFSAQSYLRYQGEKFISRFDANCYISITRKMDTNDIAREREGDLESLLESLTQPALVIGISSDGLFTYSEQETLARYMPNAKLERINSPEGHDAFLLEFAAINKLIIDFLKENCSEILNAPGIEWNENIEEVTESVFGEAEEVTNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW52</accession>
    <name>A0A1E3NW52_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_22959</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNR ribonuclease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036608.1">
        <property type="nucleotide sequence ID" value="XM_019180970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW52"/>
    <dbReference type="STRING" id="683960.A0A1E3NW52"/>
    <dbReference type="GeneID" id="30198216"/>
    <dbReference type="OrthoDB" id="372421at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048027">
        <property type="term" value="F:mRNA 5'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000900">
        <property type="term" value="F:mRNA regulatory element binding translation repressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903450">
        <property type="term" value="P:regulation of G1 to G0 transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.690:FF:000001">
        <property type="entry name" value="Cell wall biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.700:FF:000002">
        <property type="entry name" value="Cell wall biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000100">
        <property type="entry name" value="Cell wall biogenesis protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041505">
        <property type="entry name" value="Dis3_CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041093">
        <property type="entry name" value="Dis3l2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF9">
        <property type="entry name" value="DIS3-LIKE EXONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17877">
        <property type="entry name" value="Dis3l2_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17849">
        <property type="entry name" value="OB_Dis3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNB" evidence="3">
        <location>
            <begin position="664"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="57"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="410"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1161"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="83"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="96"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="308"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="427"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="448"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="475"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1167"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1271" mass="142188" checksum="A28276320BB64868" modified="2017-01-18" version="1" fragment="single">NNGYNQPSYTTTTGGKNPRQIHIAHRRSPSELTSLMIEQITLQRQLELVQQQQQQILAQQGGSGGSSGYSAGSSGNQFQPPNSHRRTGSNTHRRSQSSMTGSTTSPQNTGGSALGHNRRHSLGLNEAKKAAAQVQAQRSGKEPSTTDVEESSSSMPPPSGFKFPSTVIDAPSPPPQTQSYASVSRRPSNGGGHGRSRSSVGHSPQRSYQFPPKSPQQSSSGGFDSLAPPPQNFSYHERRQSGHQRSNSRNFDPNTQQNINMNWRSQQSNQQQQQRQLSPSRNSFTEGSSSFLEPPQPQFTPGHRIRGSHNNNNNSISSLSGFQLPGQVGGRKSLFAPYLPQSSIPDLVAEGRLVVGTLRVNKKNRSDAYVSTDGLLDADIFICGSKDRNRALEGDLVAVELLAVDEVWSSKREKEEKKRRKDNTQTSSSNNNSNNSSEIIDDVHNDASTHSTTTTTTTTSGEDDKDESTSVGGSLKRKGSLKQRPTLKKNDDVEVEGQSLLLVEEEEINDEYKPLYAGHVVAVIDRIPGQLFAGTLGLLRPSQQAKDQQSSSRPKIVWFKPTDKKVPLIAIPTEQAPKDFVENHEKYSDKLFVASIKRWPITSLHPFGTLVSELGPMNDSNTEVDAILRDNNFLCEEYLDVETGEEREDFEINDVDFEQEFNKRRDFTNEYVLAISETGVNSDNALHVKRISNDKIELGVHVSDVCAFIKEGSLLDKKARKRSSSVFLAQKVVDLLPKSINELLSFVKNEKSLALSVVFEIDTLKFEVDNVWIGESFIQPKNRLTFDQIDSILDDGDESNEYVSSASKDYIRTISLISREFRRQRLENDQLNNEVNLTLIDQIDDEKVRLHLNLFKSSLSSSILNEIFYKVNGTIAQRNYSILGDVAFLRRQTIPTLTKLESFKKKILNLGIEIDTSSSAKLINSILSIKDETIRQGVEILFYKSLSRSKYFVAGKVEAENYGAFYFNLPLYTHFTSPLRRYADLIVHRQLKQTFDQDLKLNGDNEELESLKMTAEYCNFKKDCAKASQDQSIHLLLSQTINEMSKKTGQLIVIGKVLQVYESAFDVFLPEFGIEKRVHGDQLPLKKAEFDKKHGVLELYWEKGVDSGTFIPEDEREPLSYRNSIKNKFKTSANEIAQYQQDFNKQDHDLNQELERLKLSPPSFKIPSPRTTTRSSTFEDPVSQSLPTTPVDSVKSVVGRTSSSSKIETGQGSSREKKLHALDSYLDKLTLRVEGGDYIQEIKELSDIPILLRAEIGMALPCLTVHALNPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWC6</accession>
    <name>A0A1E3NWC6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_107615</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036507.1">
        <property type="nucleotide sequence ID" value="XM_019180509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWC6"/>
    <dbReference type="STRING" id="683960.A0A1E3NWC6"/>
    <dbReference type="GeneID" id="30197755"/>
    <dbReference type="OrthoDB" id="442947at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="35"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="129"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="242"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="210"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="210"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="326" mass="35184" checksum="20327A39A9E004BB" modified="2017-01-18" version="1">MTEQEASESLFDTKFTSDASIDTSILPSTEENTTAIIGFRVLVSVKEAGIVIGKNGSVIADIRDQTGVKAGVSPVIQGCPDRILTVTGPLDSTCQALGLIAKALVSSPLDDSLFQYFPLKRLLPPGIEGSTSLRLLIPNAQMGTIIGRQGARIKALQENFNVRLVASKDFLHNSTERLVELQGLPEEIESSTKIIARCLIEDWHSAAGTTFYTPTPRTPRRSNYSSSSSNTSTSNQSNSTPGDVSRTVNFPSEYVGCLIGKAGSRIQEIRKVSGAQITIASEDDESGERTFTLSGSNRSVGKALSFLQQNLEREKQRRERIAAGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWD3</accession>
    <name>A0A1E3NWD3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SWIRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_35060</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036726.1">
        <property type="nucleotide sequence ID" value="XM_019182267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWD3"/>
    <dbReference type="STRING" id="683960.A0A1E3NWD3"/>
    <dbReference type="GeneID" id="30199513"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031496">
        <property type="term" value="P:positive regulation of mating type switching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000020">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000014">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032451">
        <property type="entry name" value="SMARCC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF41">
        <property type="entry name" value="BRAHMA ASSOCIATED PROTEIN 155 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16495">
        <property type="entry name" value="SWIRM-assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SWIRM" evidence="8">
        <location>
            <begin position="186"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="9">
        <location>
            <begin position="370"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="375"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="329"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="624"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="538"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="138"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="339"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="670" mass="75101" checksum="0BCC3257FAD8C994" modified="2017-01-18" version="1">MNEPPSSEEPKETDAEDHERIAFDTDKPEEQEGKPTSPPQNKDDESKTDEAPAPTVDETAQEKSEEEKPESKEDEEKDEEPKPDSKADQDGDISIPDANNSEYEGESDQGEEDEEPSDAEEDDAEEEDGVRKSKSRSRTAEPPKIKDENDAQNDDAKDQTDVKESSDATAVGGSKNEPYIPQTHTIVIPSYASWFDFKSIHPIEKESLPEFFTNQNPSKTPQIYVKYRNFLINAYRLNPNDYLTVTAARRNLVGDVGTILRLHRFLSRWGLINYQVDAVEKPKPVEPPFTGDYNVTYDAPRGLFPFESFKPPLEPYKLEKLKELTRGKRELNGDGNGVEAKKEEQTGEGEIKDEPIVNGGDVKRPKITKSINDGWTKEDLKKLLEGLTKFKGDWESIATHVGTHTVEQCIIRFLKLPIEDKFVGDSAQNLGPLKYAPYLPFSQADNPVLSTLAFLVSLVDPDVVKAATSNAIKIIDEKDLEQTLEKEEKGESSEVAGNGVEESARISFTTVGARSHIFKTNEEIEMNKLTNVIVNTQLSKLELKMAKLESVEKELDLEKRILQKQQEELFLDRLSFSKTSSLVISKLNNAVEKASSDPQELSRLVLEAKELLAKPARSELASFDLSKRDTKSVQPDQTTDEAENAATTVADDDLKPISIETPQTYRYWSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWF2</accession>
    <name>A0A1E3NWF2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit DAM1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Outer kinetochore protein DAM1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_64850</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="15">Component of the DASH complex consisting of ASK1, DAD1, DAD2, DAD3, DAD4, DAM1, DUO1, HSK3, SPC19 and SPC34, with a stoichiometry of one copy of each subunit per complex. Multiple DASH complexes oligomerize to form a ring that encircles spindle microtubules and organizes the rod-like NDC80 complexes of the outer kinetochore. DASH complex oligomerization strengthens microtubule attachments. Within the complex, DAM1 and DUO1 may form the microtubule connections. On cytoplasmic microtubules, DASH complexes appear to form patches instead of rings. Interacts with the outer kinetochore component NDC80; the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DAM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036708.1">
        <property type="nucleotide sequence ID" value="XM_019183818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWF2"/>
    <dbReference type="STRING" id="683960.A0A1E3NWF2"/>
    <dbReference type="GeneID" id="30201064"/>
    <dbReference type="OrthoDB" id="5586015at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990537">
        <property type="term" value="C:mitotic spindle polar microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990758">
        <property type="term" value="P:mitotic sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013962">
        <property type="entry name" value="DASH_Dam1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28113">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28113:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08653">
        <property type="entry name" value="DASH_Dam1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="150"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="150"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="180"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ57501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="213" mass="23699" checksum="24229E00E361094C" modified="2017-01-18" version="1">MSKKPSRSTTPHRLSRPSNVLPISPGVHYPIELDKTPVDDLSSQFIELNDSLVDLDSNVQQIQQIHESLNSFNESFASFLYALQVNAYVTEFNEVPTNFKEFKKVIGINEKIEKLESILNEREVPINSNTNVGDDTYMTNDDDGSFVVQPRNSILPSQPQQRSGIATTSTTARQRQSTIGASTANQGKNNSKIPVGKRVKVGGPVKDKRPPFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWL0</accession>
    <name>A0A1E3NWL0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_35233</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036710.1">
        <property type="nucleotide sequence ID" value="XM_019182286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWL0"/>
    <dbReference type="STRING" id="683960.A0A1E3NWL0"/>
    <dbReference type="GeneID" id="30199532"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034389">
        <property type="term" value="P:lipid droplet organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF282">
        <property type="entry name" value="WW DOMAIN-CONTAINING OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="322" mass="37128" checksum="DA27F958F7B0983F" modified="2017-01-18" version="1">MDFNPDDLPYTNPREERRVALVTGGNSGIGWYTVLHFYLHGFVVYIGGRSFTRVSKSIKEIEAEAIRRRSTVVENDLNQRFLGELRFVEIDLLSLKSVEAASIVIRATEPQIDLLVLNAGVMALPFALTKDNFEVQFQTNYVSHFLLTQRLLPLIKGRIIFVSSVGHHFEVFRFNRDWNFNYKPNIIFTWFRYAMAKTSGIQYMKLLALKNPDIHCYSVHPGFVMNTNLFSYWTRLPIIGVFFWFCFQVFGFFFGCSNEEGCYATLKCALSNETLLEENGSYFKAGGILTSPSRVASDINNAAYNWVWTVRQLRDRGFNDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWV9</accession>
    <name>A0A1E3NWV9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_17279</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036389.1">
        <property type="nucleotide sequence ID" value="XM_019180736.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3NWV9"/>
    <dbReference type="GeneID" id="30197982"/>
    <dbReference type="OrthoDB" id="162894at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="term" value="P:positive regulation of autophagy"/>
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        <property type="entry name" value="Serine/threonine-protein kinase cek1"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GREATWALL"/>
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    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="600"/>
            <end position="985"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
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        <source>
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    </evidence>
    <sequence length="1404" mass="153989" checksum="576619260DD773C7" modified="2017-01-18" version="1" fragment="single">LIEASAKNPSVLMELDMDGNVKHLGKAWESIIGTKVQKILNRPISNIVLGDEDDKKVFNNAISIMTTDDASYRVRFVVATNDPKPESGDGESFDSISTNGEMIELEGQGILIHNSKNIPTHSMWILKPFYANALNVQLPTKLSETLGFGADIFTSYLSALSEAGIINEDEVPAPPQVLCRICEQQVPSWWLEKHSQLCFNEHKCESEVQDAHDALVEQKQIIATINESLSKQQVNLQEEVNDYKGLPLPKVAPSDSPVARNATPLVRRGRSASIFGALRFPFKTLALLSELCEQATSINPSEFNEESDTFEFSPNTKQALEEVSSSAIPESSDPAIILLSKDTEKLVKDKVDAVSRLNSSLLYSQKIKDEVDEYVIATISETIKNIRNQTYDVVPSNPSSSSERLQTSTEKNESTNKLVYPRPTSVQSNIFTDAYLKTDNLPSPSRESIREALNETRGQYSPSRSITPNEALKDSNQEDHVPSFSDLNIKSPILTPQRKGSPGAPPGFHNSPMSSIQRNVKSSGTPSFEHQVNTPFSSPLILAHDPSDHFEKRSSGDNNYLSISSAGSGSISTGHLGKPPLSPLLVAVAPKPSAPSIKDYEVLKPISKGAFGSVYLARRKVTGDYFAIKVLKKADMIAKNQVTNVKSERAVMMAQSESPYVAKLYSSFQSKDYLFLVMEYLPGGDLSTLVKMLGNLPDEWAKHYIAEVVIGVDDLHKKGIVHHDLKPDNLLIASSGHLKLTDFGLSRMGLVRRQERAHKHSIPGDDAFAQAAMSSHHRKSSSVTPFSLSASGPPSANDFSSAGSSPTSSFFENMIKGEKKKEERSGSGSSVVDSPLLRPLHRSASQSSFALEENPSFPSTPTTKNLALFDPENTTFNKKFVGTPDYLSPETIQGTGENEASDWWSVGCILFEFLFGYPPFHAKTPEKVFENILNGRVDWPNLAPEEELAYCSPEAKNLIKRLLDMDPETRLGVNGAQEIKDHPYFSGVDWDDLYNSAGSFVPVVDDPESTDYFESRGAALHEFPSDGSDSDADDSMDSKNDAPRTQVNSTTALGSPSTMNSPTSKHLSLAIPVHLRERRPSKLNDNSGEFGSFQFRNLPALDKANKDVINRIKAEHLEHRSSMSSSSSESGTRSRNYSISSNNGLRRAVSPSSSIMRTHSPSRMSINSANAPQSVPLHERLNSSASRSGRSSPRSDSGSPSTKSFAKPQTPTPQGQPTNPSRVSRSNLFHRAFSDFSPSSSDTEDSRSSALQRVRKRRQSSKFSESSNSRLSILDILLCEPIPILRYSMKKNLESLGCAVVAVSAGDEIVRRATGEVKFDLIITALKLPKLGAIDIAKLLKHTSSVNSETPVIAATAYYKEATTANIFSDVLEKPVGRKQLKGILEKHCRWKIDRAEEAVSDTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX64</accession>
    <name>A0A1E3NX64_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleolar 27S pre-rRNA processing Urb2/Npa2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_107217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_019180495.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:ribosome biogenesis"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Ribosome_Biogenesis_Reg"/>
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        <property type="entry name" value="Urb2/Npa2_C"/>
    </dbReference>
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        <property type="entry name" value="UNHEALTHY RIBOSOME BIOGENESIS PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15682:SF2">
        <property type="entry name" value="UNHEALTHY RIBOSOME BIOGENESIS PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10441">
        <property type="entry name" value="Urb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleolar 27S pre-rRNA processing Urb2/Npa2 C-terminal" evidence="1">
        <location>
            <begin position="926"/>
            <end position="1092"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10441"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ57738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1092" mass="125333" checksum="5007A857C4EC23F8" modified="2017-01-18" version="1">MAQKEWSAEGITRFLRSKDTSIEDIIQVSNDLLDDKLDVYLPNKVIFVFELLCDRLGDNKYKKYRVNPEIWLLFNKTWTLLDSQPKIRNRTFQGLRFGEALASALSHTESSKQFLETITTTINFVRFNSVISNNVFDFSLDILTQYLELLNKLSDELEEEEVSIKVNGISSFLQSIISTEKTSKKFTTNFASTSLPSILRLSEKRQQQYPQLLALIKKILLSKEQQESLIPNLVSLINTKPSTGAIVNYYRIIIDTISKNDIESAEKMFTLITEKHPTASEQLLGYLSSIKRTLSQTFLDTIIDKEFSNNQQNWNLVSSVVQLDIEIGIKNSEKIMTSLEEQVYEEDLKLGEQLVNSFIRARELVNFFELWLKVIKANPTSRWLKSEFTEVVSHNIHVLSFSQLKTLIDLLLSSDVDVAELTLLNTVVQGLFKTQDTVLVQQARELFSPIWSINQDQDQLWYLKFQLLCLYEDILDSSGFEKIIKDSKSPKNINHYYTLFRTREIFEYDISPLAPKFLSFLKKSKLTSEILQTVFTRWFVLVNLLFTSEQIEQIVDLLLSDVNSASKIFENDLLFEQQKITETLINQISSSFSDKKADKSFKIQVLQLIPIQCYPRRLKSLILDQLVDEVLSKHSSNKQIILETIQHILQTPTFRSKIESDISTIYKIVDTFPETNLFEKIWPHYIQQIKETTSQDFISSIIDHVTQSFSQNKSNKLSSSFYIGFTILSNPPCTDASNVLELKSAFVNASKRLLNSFIDKSKGKNSEISWLLNVLYQLNLSIEDFKLLKPSIQNFGKQLTEGDNVAKTNIFLLFSKFPQTGDFAIEYFEALYIVLRDSGISSEELLKGLGNVVSSLTQEEFSKSFKVLIESFNDNSDNSLYFVEILSIYWKFFQRDSPDSIKFFINSLSTVLLNFHKISHSQGLIHILNSIKSLLVEKTWIITQYGLELIITLLSKTADSLLISNNIHSEEIYQSLTTTLSTILLIQRYRLSNRHHLIISLFKSLLACLSKRQHNGVLSSSINAGEKFARVLTNLCEPSQHSFKEASNGLNSSTSLAKKALRKHLAVLLLSYIYFALKYSFDSHVREAILPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXD2</accession>
    <name>A0A1E3NXD2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Nucleoporin NSP1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_81134</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037010.1">
        <property type="nucleotide sequence ID" value="XM_019185180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXD2"/>
    <dbReference type="STRING" id="683960.A0A1E3NXD2"/>
    <dbReference type="GeneID" id="30202426"/>
    <dbReference type="OrthoDB" id="2538017at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140693">
        <property type="term" value="F:molecular condensate scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990000">
        <property type="term" value="P:amyloid fibril formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025574">
        <property type="entry name" value="Nucleoporin_FG_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024882">
        <property type="entry name" value="NUP58/p45/49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13437">
        <property type="entry name" value="NUCLEOPORIN P58/P45 NUCLEOPORIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13437:SF2">
        <property type="entry name" value="NUCLEOPORIN P58_P45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13634">
        <property type="entry name" value="Nucleoporin_FG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="8" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="60"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="297"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="162"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ57803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="527" mass="53197" checksum="3961953FE5331483" modified="2017-01-18" version="1">MFGSNSGTSGFGRSASATGLFGGGAPANTGFGSSSTNTGNTGGGGLFGAAKPAATSGGGLFGSSSNTSSGSGGLFGSGNNQSNTNPGSGSGGLFGNTNNTSNTTGGGLFGNNTSNTGTGFGLGNNTSTTGGGAFGNTNNQSTTNSNAGGLFGSNNTQAPAASSGGLFGNTSNTTSTNNGGGGLFGANNSTNKPAAGGLFGNTGASTGFGANNPATSNTTTGGGLFGSKPAATTTTGGGLFGSNNNAATSGAQSGGLFGNNNSTNNNTTGGGLFGSKPAAPSGGLFGNNNTANTTANTTGGLFGSNNTTTNTGSSLFGGSQPLQQQQQQQQQQLPQITPLTTVGDLPEQFRREIEQLDQYIQSQVQISEQLKAEQDEHTEVIDSIPRDIEFLQNRYSSTNQALDNDLATLQDIRASTDEALHDSENFFQILQRLLAAGSKISSIELDVYFNKKVEFYKSKLEEYTRVLGEIDAAVEGIEKYSISDLGGIDLIISTLHEEFNLFMEVANTVADIHQKVKTLSERSTGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXG3</accession>
    <name>A0A1E3NXG3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_101736</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Mitochondrial peptide exporter (TC 3.A.1.212) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036569.1">
        <property type="nucleotide sequence ID" value="XM_019180331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXG3"/>
    <dbReference type="STRING" id="683960.A0A1E3NXG3"/>
    <dbReference type="GeneID" id="30197577"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18573">
        <property type="entry name" value="ABC_6TM_ABCB10_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000058">
        <property type="entry name" value="ABC transporter B family member 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000218">
        <property type="entry name" value="Multidrug ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF1">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002773">
        <property type="entry name" value="ABC_prm/ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="257"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="337"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="375"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="101"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="433"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="58"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ57362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="710" mass="78403" checksum="587E0D43287195C9" modified="2017-01-18" version="1">MMLPFRSLASGIRLPLSRITPKLTHPHPSTSLIQCSKYHTLFNKNQSIIPRTPLTILQRYNSTNPPSPDTTKLSPHDSKASGFQDIKRLFELAKPETKPMIGALFLLMISSAISMSVPLIIGKFLDSGNENKEDDNEDKKEFGDTKIYGLTMNQFFGAIAGVFVIGAVANSGRVIILRTIGEKLVARLRTRTLKKSLEQDASFLDNNRVGDLISRLSSDATIVSRSVTQNISDGIRAVLSGGVGLTMMSLVSWKLTSIMALLAPPLGVMAFFYGRRIRNLSRNLQKSIGDLTKVAEEQLNSTKTIQAYGTENLEVSKYSENVREVFKIGYKEAKLSGIFFGTTGLIANVGLITLLGIGSSMISNGSLTVGELSSFMMYAVYTGSSMFGLSNFYSELMKGAGAAARIFELNDRKPEIPLTKGLKPSLKDLSSTIKFKNVSFWYPTRPNIQIFNNLTFEISPGEHVCIVGPSGSGKSTITSLLLRFYDVQKGEVLINDKNLKNFNLRSFRKKIGVVQQEPSLFSGTILENITYGIHNYKEQDLQIALNKANVLKFINEFPDKLDTIVGPRGTQLSGGQRQRIALARALLLDPPLLILDESTSALDTKSEKIISETLNERCKKGLTTISIAHRVSTIKNSSRIIVIGKDGQVVETGSFDDLISIKNSFLNQLLSKSEDEQYVEVKDQPSNEQVLEEIEENVEIQTEKDLSLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXN3</accession>
    <name>A0A1E3NXN3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_56460</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037156.1">
        <property type="nucleotide sequence ID" value="XM_019182782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXN3"/>
    <dbReference type="STRING" id="683960.A0A1E3NXN3"/>
    <dbReference type="GeneID" id="30200028"/>
    <dbReference type="OrthoDB" id="3064516at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05674">
        <property type="entry name" value="M20_yscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000027">
        <property type="entry name" value="N-fatty-acyl-amino acid synthase/hydrolase PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017141">
        <property type="entry name" value="Pept_M20_carboxypep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047177">
        <property type="entry name" value="Pept_M20A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE/HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962:SF1">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE_HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037217">
        <property type="entry name" value="Carboxypeptidase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="8">
        <location>
            <begin position="278"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="539"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="570" mass="63898" checksum="4E79C255462F4858" modified="2017-01-18" version="1">MTEFQPVTTPVKKRKLNIYKKILASIVLGGLFIYQASNHIKSCSQTDNTIYDETTPVVAVRPPNYNSDFNSAFKNYFDEEFRNKSIAKLAGAVRIETESYDNGQSPVDDITNWIKFYKLHDYLKDTFPLVYETLKVETVDKISLLYTWEGSDPDLKPLLLTAHQDVVPVEPKTKNLWTHDPYGGVFDGEYLWGRGSSDCKNLLIGTLEAVEQLIKDGFKPTRSIILGFGNDEESTGFGASALNRTITERYGSDSIFAIVDEGTGVINYDDQYIAIPSTAEKGHLNVFIELTTPGGHSSVPPDHTSIGIISRLVALLEDHPFDPILSAKNPLSQFLKTVTKDSKNVPDSLKKDIQNLDKDKSANERVIEYLSKEPSTNYAIRTSQAIDIIHGGVKSNALPEYVTLLINHRISADSNSKETVDHFLTHLKKISKQFDLGIILDGEFIIPSTKNGVFNVSAQDPLEPSPISPIDGEIWDVFAGTIKHVFDDIVLKDTNETLKIAPYLMVGNTDTRRYWGLTRNIYRFQAQLAKSEDLTSGIHSVNEHISVDQHLQVLTFLYEFILNVDKVKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXP6</accession>
    <name>A0A1E3NXP6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Monopolin complex subunit Csm1/Pcs1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_80090</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037133.1">
        <property type="nucleotide sequence ID" value="XM_019185001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXP6"/>
    <dbReference type="STRING" id="683960.A0A1E3NXP6"/>
    <dbReference type="GeneID" id="30202247"/>
    <dbReference type="OrthoDB" id="2431049at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034506">
        <property type="term" value="C:chromosome, centromeric core domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033551">
        <property type="term" value="C:monopolin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990644">
        <property type="term" value="F:microtubule site clamp"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045144">
        <property type="term" value="P:meiotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23787">
        <property type="entry name" value="RWD_CSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040349">
        <property type="entry name" value="Csm1/Pcs1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020981">
        <property type="entry name" value="Csm1/Pcs1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038608">
        <property type="entry name" value="Csm1/Pcs1_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28006">
        <property type="entry name" value="MONOPOLIN COMPLEX SUBUNIT CSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28006:SF1">
        <property type="entry name" value="MONOPOLIN COMPLEX SUBUNIT CSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12539">
        <property type="entry name" value="Csm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Monopolin complex subunit Csm1/Pcs1 C-terminal" evidence="3">
        <location>
            <begin position="229"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="183"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="100"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="324" mass="36951" checksum="E22760696844D267" modified="2017-01-18" version="1">MPPKRKTGTRSTRRNKKEEEEQDQQLIEEDVPEVEEPPKKQARGRGKKKKVEPAEEKQEQSEKELTKENIEPEEEPAVPSKKQQSKVSPITTSKSIAPASPSKRVSSISSRIPSPVKKHPLGSNKPDLEFKKTEQAINQNFVSKNLPKLSSIIDDLKSSKADALFDELKCSTETRFKTSDDLINSLTSRNQQLSIELEELRGRLSELENNPNGDVQPIANEEESFQNELILDMMEQIVGLRIHSVEDTDEALSFDCSQSGKNGVLDYKLTIPKEDSSEIIYTPVNKEESNDLKKYLPEYFFDDLTFPIDTLQQFYHKIYRGLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXY4</accession>
    <name>A0A1E3NXY4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Altered inheritance of mitochondria protein 36, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_80887</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036759.1">
        <property type="nucleotide sequence ID" value="XM_019185138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXY4"/>
    <dbReference type="GeneID" id="30202384"/>
    <dbReference type="OrthoDB" id="4081130at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="27728" checksum="C70E19BDF359AEAB" modified="2017-01-18" version="1">MLRGGFTNFRPLCQQSTLRSIRTPTPLLKRCINNSSKPDPDRGPRFRNFIALGIISYVILAQVVEAVNKENPRNKSMSESEYFKQQMKLKRRKAIFTQDEKSVWFLKSDDPEKLKSLKIDGFEILDPNVLLEKEKQDKDSLYYALLNDAEYKTLPTGLLVDLISKQLKASDSKKFIVINYPSDIKESVKFEEKVVTVKKLIHLKSSQDKGDDDDDVVKYYKTVNKVDEIKSDKDITKLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY12</accession>
    <name>A0A1E3NY12_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Selenoprotein O</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_101407</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SELO family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037288.1">
        <property type="nucleotide sequence ID" value="XM_019180321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY12"/>
    <dbReference type="STRING" id="683960.A0A1E3NY12"/>
    <dbReference type="GeneID" id="30197567"/>
    <dbReference type="OrthoDB" id="10254721at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070733">
        <property type="term" value="F:AMPylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00692">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003846">
        <property type="entry name" value="SelO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057:SF14">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02696">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="571" mass="65053" checksum="DC54ABD08E31CF81" modified="2017-01-18" version="1">MTKIPLKEITKLFNRPRTLKSGAYQFTVPEKRPSYKHLLSSPKALEFLSLPPDSPSDPYYTSIVTGESFYNDEKIWPYAQAYAGFQFGQFAGQLGDGRVSNLFTLETPTQGKQELQLKGAGKTAFSRFADGKAVLRSSIREFIISESLAGIGIPSTRALSIGLLPDTKAQRERAEVCAVVARFAKSWIRIGTFDLYRWRQDREGLRDLCDYVISDVLETLPKFTPGEFEKDEYKEVTEGEEIVDGLIKITDSSRYDQMYRAIVNLNAKTVAYWQSYGFLNGVLNTDNTSIIGLSIDFGPFSFMDQFDPNFTPNHDDVQLRYSFANQPSVIWWNLTRLGESLVELLGAGPDIVDDEFFINKGIKKDQEDHVIKRVSSIIKLAGVEYKKTFMETYNSLMTQRIGLKEVKPGDNDLIARMLEVLVKTKVDFNKFFIKLQNSQDLDDISTYLTPELVERIENPQDKVDHELNEELTTTINSFIAKYKERLGSTSYEERYSIASKVNPQFTPRNWMLDEVITEMYDDPNDVSKLSKLLKMSSNPFDPSQWGEELKDVEKHWVGSEGTTMIQCSCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYC3</accession>
    <name>A0A1E3NYC3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_64138</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037197.1">
        <property type="nucleotide sequence ID" value="XM_019183674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYC3"/>
    <dbReference type="GeneID" id="30200920"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="32016" checksum="96C87769356FAF3B" modified="2017-01-18" version="1">MFINRGEEFSKLDKEFSQIIKNTKLKVVSLPETNRSILLLPAVVYPDRVTNKYLIEADSFKEFFDLNGDELHRLQNTNNYRDDVFLKVYDIREIFEYYFRNIEHIAEMDPLITSDEAYHRKVISQFTREVNINKFIRSHNASCSRGEDKINCPDMLSYGAIIRENTFYGFYICFQYIKVHERLSRSDIPNITHEVEKLHKVGITHGKLMENISVNEDGTVTFFDFASAHRLDEGTSKLEKHSDVCNDFLSILRLSGHLEKSKDGFIKNPATRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYE4</accession>
    <name>A0A1E3NYE4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_22629</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the type 2 lipid phosphate phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036929.1">
        <property type="nucleotide sequence ID" value="XM_019180956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYE4"/>
    <dbReference type="STRING" id="683960.A0A1E3NYE4"/>
    <dbReference type="GeneID" id="30198202"/>
    <dbReference type="OrthoDB" id="301434at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03388">
        <property type="entry name" value="PAP2_SPPase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF28">
        <property type="entry name" value="DIHYDROSPHINGOSINE 1-PHOSPHATE PHOSPHATASE LCB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="9">
        <location>
            <begin position="80"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="432" mass="49555" checksum="F8CFA086174F8624" modified="2017-01-18" version="1" fragment="single">DSGNYPHIHYKTKIHPLRFKFRQFLLPYIRAETPILAKIQKNSRNSFFDYYFSYTANFASHTFYVLMLPLPIWLGYGQVGRDLVFIIGYGIYFTGFLKDFCCLPRPRSPPLHRITLSGYTAKEYGFPSSHSANATAVSLFLLKKLLENYNNFQSSLTPNILLFVLAIYYISLIIGRIYCGMHGFSDIIVGSLIGCICLVVRTLTQEWYDSLILQKSILIPICTTLVNYFLIYIHVSPVDDCPCYDDSVAFIGVIMGLEISHWGYIKTSYSLDHTYGVDPLDIPFSFAHSGILKVLLRIFVGVSLVVTWKEISKPLLFKIFKPFYDLIVTNDKESLTFNRIRSDTLERKEKLTDAKGLIKDIGNPRKRDSVGPMSSIDRTEIEELQKHQNYFAELDKLHETNVIFTCGAFKPRYDIEIIVRLIVYAGIPIVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYZ6</accession>
    <name>A0A1E3NYZ6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_12462</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037471.1">
        <property type="nucleotide sequence ID" value="XM_019180533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYZ6"/>
    <dbReference type="STRING" id="683960.A0A1E3NYZ6"/>
    <dbReference type="GeneID" id="30197779"/>
    <dbReference type="OrthoDB" id="5368598at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023041">
        <property type="entry name" value="Glucose_rcpt_Git3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022596">
        <property type="entry name" value="GPR1/2/3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF37">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR GPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11710">
        <property type="entry name" value="Git3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11970">
        <property type="entry name" value="GPR_Gpa2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="13"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="6">
        <location>
            <begin position="8"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="303" mass="35082" checksum="84CB1F39373D9FE7" modified="2017-01-18" version="1" fragment="single">ETFTHNQLNALRIITITTSSISILSCLITFYTYLAIHPSRKKFRHQLIIFLIIFDFLKALAILLYPILSLIKNTKQIGPRTINFLGFFTAFATEGGDLVILSFAIHTLLTIFYPTQTGGLYIIRYYIYVLSIVGPAILASLAFINNVGYVELDIWCYLPERPIWYRIALSWGPRYGIMLAILIIYCTIYFYIISQLKKLENIQTSASGNGNNNDFNINLQIGNEEKLKLRYKVMARQIKIIFLYPIAYFFLWMFPLVSNSLRLQLKSNYGIAVLVSFMQPFNGTVDTLVYLYREKPWNLTYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ18</accession>
    <name>A0A1E3NZ18_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_64102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037159.1">
        <property type="nucleotide sequence ID" value="XM_019183671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ18"/>
    <dbReference type="GeneID" id="30200917"/>
    <dbReference type="OrthoDB" id="1555154at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="231" mass="26540" checksum="7D96B6B0FC6D9A1D" modified="2017-01-18" version="1">MNPLIRFLLPKLNSPFTEFYFPFVRKIEGFEDLRLLDNTNCYFSQLEVLTIGSLSFQLFNLKAPKLKVFNHSKVILDRTAFNENVSTARISYSKFLDNLMCSNLECLEVIGAVAHSTLYKFNSVKILRIYADNSSESFNLEEYKSLEELVISKCSKLRSIEGLHCNSQTLRLLDLSNNKKFDQDISGFFTNMEVVNLSNTYIQKYPNLETSDNHLIVVNVEGCKNLNIKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZG9</accession>
    <name>A0A1E3NZG9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_85455</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037621.1">
        <property type="nucleotide sequence ID" value="XM_019185893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZG9"/>
    <dbReference type="STRING" id="683960.A0A1E3NZG9"/>
    <dbReference type="GeneID" id="30203139"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010995">
        <property type="term" value="P:free ubiquitin chain depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02659">
        <property type="entry name" value="peptidase_C19C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000044">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024729">
        <property type="entry name" value="USP7_ICP0-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029346">
        <property type="entry name" value="USP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF644">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14533">
        <property type="entry name" value="USP7_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12436">
        <property type="entry name" value="USP7_ICP0_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="MATH" evidence="8">
        <location>
            <begin position="18"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="182"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1119" mass="130312" checksum="C0ED06ABA08BEE32" modified="2017-01-18" version="1">MSTRITKDPNEEDEIMKKGQYTWEIKEYRALNEAKVRGPTFTVGGVEWNILLFPRGNSNQHLALYLEPVQPKKTNPETGEEEPLDPNWYVCAQFTLVISNPKDPKNYILNTSHQRFNKDATDWGFSNFTDLKALYQSRKDHGPLIWEDKVNITAFVKVLKDPTGVLWHNFLDYDSKKETGFVGFKNQGATCYLNSLLQSYFFTKSFRKAVYQIPTEGEDPNDSVALALQRIFYQLQHSQDPLDTTELTKSFGWDTGEAFTQHDVQEMNRILMDRLEARMKGTVAENSLNNTFVGKMKSYIKCINVDYESSRTEDFWDIQLNVKNLSNLTQSFQNYIEVELMNGENQYAAQDYGLQDAKKGVIFESFPNVLHLQLKRFEYDFNYDQLVKVNDRHEFPESIDLSPYLEHQEGSNPDPLIYDLHGVLVHAGDITTGHYYAMIKPDQSNNWYRFDDDKVWKVTRRQVFDENFGLDPLDNTKLAKMTRVQYQNYQYRRHTSAYMLVYINRNESANILQEVTESDVPTYIVDEVKKELEERERLRKELEESYLYLNINFYTNKLFKKYEGFDLGPYTEEDSPYTLRVLKEKKFTEIFTELKESLDIDGESVNFWIMNYRRNQTLRPSSQLQYDNLTVGEIYEKFLSKKHSSANLWIEEPENDLNTAIGEESTVAETQETELVTSSEKILMFLKYFDPENQSLKGLTHFYTDSDATIESLIPKINKYLNFDPDQTLDLFEEIQPTSIEVLVPSYSFYKAELSNGDILTVQKSVLNNNEDILSSNSRDIYPFYDSANLYYEYLRTRIYVKAQPLADTSNDDEYVVKKDTDELFDFWISTHAPYEELATRISKVAKVDPKYLRIFVNYSGNRIPLRTDMFVGNVTPKSVSALTPLFEYEVLNISLHELENMKSIKVHWLTSGYFHYQLLDILIPKTGTIQDLIDKIQHKTGFDAKDKENCMVWANVNFKFFQVLYPEIQVASLENTLTIFAAILPEETITLEKQFAIEEGDQIDDNGNKADHEKLVPVIQFNKDPTRQHGISFVFKLIPGEKLSETKKRLQKKFGLGTKEFSKVSLCAWDTQFKKPLYLEDDDLVTYDEITESDYLCMDHPNRTSRQGSLQGGGISIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZH3</accession>
    <name>A0A1E3NZH3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PH-response regulator protein palI/RIM9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_63101</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037796.1">
        <property type="nucleotide sequence ID" value="XM_019183490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZH3"/>
    <dbReference type="STRING" id="683960.A0A1E3NZH3"/>
    <dbReference type="GeneID" id="30200736"/>
    <dbReference type="OrthoDB" id="2354757at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035838">
        <property type="term" value="C:growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051380">
        <property type="entry name" value="pH-response_reg_palI/RIM9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28013:SF8">
        <property type="entry name" value="AEL027WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28013">
        <property type="entry name" value="PROTEIN DCV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="486"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="347"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="491"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="518"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="536"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="621" mass="67080" checksum="F37ED938408F606E" modified="2017-01-18" version="1">MGLVFGCSTVVVVINAIALAFLILATITAPVVSMLGLAEADNVKYGVFGYCSSSGSNCSDATFPYKVTSLSPNTNDWKMDDSARDTLAKILIVTPIAAGLTLFSFIFNLFAHFKAFGSSLAFYLIGFFFTLLAFFGAAITCIVTFLLFFPHVTWCAWILIPAAILNLFAVPLMFFALRTTPSSFKDDDEEGEVEDKNLTDLSDGDNFKSNNEFKLDDFTGPNTTFTNSSIDKNIPSSTLKVITNDTSSMTDQRSGLEKATAHDYSTNKNPTKDFNYNGQIQTQHYGNNNLNNEGTPNLVSSQNYNRGGAVRSEAPSIKPQSAKPDYSTAGASLYPKSAYQSQNSAVPPSRSQQRSQYQAPQRPLNQFQSLQQPGSLPRNATSTSSSGLNTPGAQNTNAIPSSVSQSGVPSGVPTTSSSVYNNTESNYNSANGLGNSNHGSSASDDYDNELANDNDSDFTSVSQRAVNPRYYRGANAAPTLFPNQEQQQLVSPSQGPGSYFPSGQPQQQQYQQQPYPDYTSSHYSGSAVQPGPYSPQPQFQPGSAPLPSQHQQPPRKTASDVLLSTNPDFMVSGPAFQRQQFPRRANPQATGSYRPAYKRVQGGNSKITPASLGGDSPYNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0C2</accession>
    <name>A0A1E3P0C2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_79334</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019184873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0C2"/>
    <dbReference type="GeneID" id="30202119"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="586" mass="68548" checksum="01461B9912FF08A1" modified="2017-01-18" version="1">MIKLESKVSVTSPAIRSLRAKSQEELLESFINVQQYDKLLYYLESSLFNNAPTLNCPSAFVINCLFTLATLGEYYNADLIRKEFDKPLSSQPLHIRAINILKRLMCIVQQSSEKGDQTFHRTEYNADDVNYTKSQLIRALELIIRTDKRDLRPRILRKDYKEKKEPEPEADEFTNTQPSDNEKVNSTDWSPQKKLLEQQFQLVKGPGADSDDESESEGENILDRKSYMVLKNERFDIALQNDSFWAAVSWALECSTSRDKVRLEAWKVWRPILNILIDIATIDLEDFISAKKSNTDGTDGMPAMFTETLAYKLLIMVGRYNPMDNLLDVIFPKVEQTMNKRDKKVGPIFANELTTARSVHLVDFLFQRPNDERISMESMLLRKKLYFLTYRAANDANPDDCFKESRFSSWKLARYLTQKLVDLSYVDFKKFFILQESELSETWKLDTILDVLFDWFVQINGYVFDTPTLDFLEDSDPEFMSSFLCNVFYEKEFYKKHTEADSLLKHMQTDFSKLNFSLYFLLNLWLKFSNFLSTEKEQKIKEIVRWCETGESKRRAALQHAKPKDKNVHKNLFSLVQAVKAQFNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0Q8</accession>
    <name>A0A1E3P0Q8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="5">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">2-oxoglutarate dehydrogenase complex component E1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_55455</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 2-oxoglutarate + H(+) = N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:12188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:20092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="1.2.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="5"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038266.1">
        <property type="nucleotide sequence ID" value="XM_019182760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0Q8"/>
    <dbReference type="STRING" id="683960.A0A1E3P0Q8"/>
    <dbReference type="GeneID" id="30200006"/>
    <dbReference type="OrthoDB" id="413077at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02016">
        <property type="entry name" value="TPP_E1_OGDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1150:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12470:FF:000003">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11610">
        <property type="entry name" value="Multifunctional 2-oxoglutarate metabolism enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1150">
        <property type="entry name" value="TPP helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042179">
        <property type="entry name" value="KGD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006914">
        <property type="entry name" value="PRK09404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008907">
        <property type="entry name" value="PRK12270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF4">
        <property type="entry name" value="2-OXOADIPATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="17">
        <location>
            <begin position="638"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ59059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1005" mass="113191" checksum="A15006B3D2466C7D" modified="2017-01-18" version="1">MLKTLRYRGGSNLPRARLINQLRSSNLIARRSLATGTDSFLQTQNATYIDEMYDAWKKDPSSVHVSWNAYFKNLDGNVPPAQAFSAPPTLVPTPAGGVATHIPSSGANVNDDVLLHLKVQLLVRAYQVRGHQKAHIDPLGISFADDKSVPTPKELTLEHYGFTDADFSRPITLGPGILPRFAKDGRTSLTLKEVIDTCERLYCSTIGIEYIHIPSREKCDWLRERIEIPQPYKYTVDQKRQILDRLVWATSFETFLSTKFPNDKRFGLEGAEAVVPGVKSLIDRAVELGVEDVVIGMPHRGRLNMLSNVVRKPNESIFSEFTGSVEVDEGSGDVKYHLGMNYQRPTTSGKRVNLSLVANPSHLEAEDPVVLGRTRAIQHYKNDVGTFDKAIGVLFHGDAAFAAQGVVYETIGFTALPAYATGGTVHVIVNNQIGFTTDPRFARSTPYPSDIAKAINAPIFHVNADDVEGLTFIFNLAAEWRATFHTDVIIDVVGYRKHGHNETDQPSFTQPLMYQKIAEKKSVIDVYTKKLIDEGSFTKEDIDEHKQWVWNILEESFTKAKEYKPTSREWLTTPWEGFKSPKELATEILPHLPTAVEENTLKHIGEVISSWPEAFEVHRNLKRILGQRKKAVESGEGIDWATGEALAYGSLVNEGYHVRVSGQDVERGTFSQRHAVLHDQKSENTYTPLKHVSENQADFIISNSSLSEYGVMGFEYGYSLTSPDAFVQWEAQFGDFANTAQVIIDQFIAAGESKWKQRSGLVLTLPHGYDGQGPEHSSGRIERYLQLTNEDPRYFPSEEKLNRQHQDCNFQVAYPTTPANLFHLYRRQMHRQFRKPLALFFSKQLLRHPLARSPLDDFSKDSHFQWIIEDAEIGNKIGTKEEIKRVILSVGQVFTALTKKRETLEDKSTAFIRIEQLHPFPFAQVRDALNSFPNVEDIVWCQEEPLNMGGWSYAQPRLQTVLKETIHNDKIVRYAGRDPSASVAAGTKSMHIAEENAFLEDAFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0R6</accession>
    <name>A0A1E3P0R6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_79405</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038067.1">
        <property type="nucleotide sequence ID" value="XM_019184887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0R6"/>
    <dbReference type="GeneID" id="30202133"/>
    <dbReference type="OrthoDB" id="3981101at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="751" mass="86550" checksum="9E404F12A6D6352A" modified="2017-01-18" version="1">MLIPKNILGITRRRLQNTSTRRKLYYAEHIPGPSIPPFPIPPASTLRRGTPIRQQPVEMEKDDAQDQVNGMLDKLINGMKSFEDVGVENDRVEQTRTKQLNFNLLPLKLLEKLNKTSDYHVKLSCLLDYRYLLKVKPEFVENTLFKLGIETAEDICDSLSEDLVLKWVKFQLTHKDFTKAGQVTNQIIMRRNGDLDVEKLCATVFNTLRSDHDLKYGVLDLMRALYELLDHQNTLFKFVYTTLTNNEEIMVSNGVEYKFEDIWKMIQLCRVIEEVADVRVLPEPYWKTDFDYINLILGLKCFKAGDLERGFSHLKRRKVIGNDGVVYRVIKAGLENKTKNTTSTKTMAKPTTFENRVLKVIISNNERHLSPIDLLTFQKFSKNIYTFNNSIKELTNKETVFALKNVCLKKFLDSGLLKLAQNMVANNLDQVYKFEVSYLATVFHLANDSQLLGEVVSRLPTVTKSSFLSVLFTQQLKIEQSNYHTKESKIPERQFEKIQWILEAVNYQLCDKTFRKITPLLTKLPLKLQQSIITKISGPNFPLYIKYLSSGIPLSSTLIPILVNTGKCRTRSELQSLARYISRSCSVEEIGYILSNIKEQIRYDSLHLKITDALVRFGEPDFEKAKQLIEEHPHDRSKFLLNQVLLQKNETIINYSDSYVKKPIELVKLKALYRLTDDTKLGYGSSLRAQVGRLIMANKFDKNMAVLLLKKIVSKIKSNGELKNTERLKYGVAICQRYGVSEEVISEILHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P101</accession>
    <name>A0A1E3P101_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_24602</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038091.1">
        <property type="nucleotide sequence ID" value="XM_019181041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P101"/>
    <dbReference type="STRING" id="683960.A0A1E3P101"/>
    <dbReference type="GeneID" id="30198287"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051000">
        <property type="entry name" value="Homeobox_DNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324:SF9">
        <property type="entry name" value="HOMEOBOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324">
        <property type="entry name" value="HOMEOBOX PROTEIN HHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="6">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="3"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="62" mass="7521" checksum="26927AFD57FBF515" modified="2017-01-18" version="1" fragment="single">ARRKRRRTSPLELSILQAEFEKGQTPNRTRRLDIAKRVNMTEKAIQIWFQNRRQTLRRQSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P150</accession>
    <name>A0A1E3P150_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Nucleolar GTP-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_32456</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Involved in the biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Nucleus</location>
            <location evidence="2 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. NOG subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038416.1">
        <property type="nucleotide sequence ID" value="XM_019181966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P150"/>
    <dbReference type="STRING" id="683960.A0A1E3P150"/>
    <dbReference type="GeneID" id="30199212"/>
    <dbReference type="OrthoDB" id="415015at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01897">
        <property type="entry name" value="NOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1190:FF:000001">
        <property type="entry name" value="Nucleolar GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000496">
        <property type="entry name" value="Nucleolar GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024926">
        <property type="entry name" value="NOG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010674">
        <property type="entry name" value="NOG1_Rossman_fold_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012973">
        <property type="entry name" value="NOG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45759">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06858">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08155">
        <property type="entry name" value="NOGCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038919">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="OBG-type G" evidence="10">
        <location>
            <begin position="168"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="575"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="590"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR038919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="639" mass="73689" checksum="E94FEC5D9AAA6549" modified="2017-01-18" version="1">MQLTWKDIPTVPEANDLLDIVLNRTQRKTPTVIRPGFKITRIRAFYMRKVKFTAEGFDEKFQDLISGFPKIDDVHPFHRDLMDTLYEKNHYKVSLAAVSRSKTLIEQVARDYIRLLKFGQSLFQCKQLKRAALGRMATIVKKLKDPLTYLEQVRQHLGRLPSIDPNTRTLLICGYPNVGKSSFLRCITKADVEVQPYAFTTKSLYVGHFDYKYLRFQAIDTPGILDRPTEEMNNIEMQSIYAIAHLRSTVLYFMDISEQCGFSIEAQVKLFHSIKPLFANKSVLVVINKTDIIRPEDLDQERQDLIQTLTQVPGVEIMTTSCHEEDNVFQVRNKACEKLLTSRIEQKLKGTARVNNVLNKIHVSRPTPRDELKRDAFIPEGVAKLTKYDIEDPNRRVLHRDIEAENGGAGVYNINLKDAYLLEDEEWKNDKMPEILDGKNVYDFLDADIAAKLQALEEEEEKLENEGFYDSDSEIEDEEVEDIQEKAQWIRDKQQKMINAARNRKALKNGGTMPRSKLTKNYSELEEHLQSLGHDTSILNSKKNKKLGDRSYQETGAEVLQQEFQDFADYKKQPAYKSDRLNDGVSDGALRSKADRLAKIQRRERNRQARQGEADRHATASLPKHLFSGKRGVGKTDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1B6</accession>
    <name>A0A1E3P1B6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">2-dehydropantoate 2-reductase</fullName>
            <ecNumber evidence="2">1.1.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Ketopantoate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_33374</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.169" evidence="2"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038402.1">
        <property type="nucleotide sequence ID" value="XM_019182064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1B6"/>
    <dbReference type="STRING" id="683960.A0A1E3P1B6"/>
    <dbReference type="GeneID" id="30199310"/>
    <dbReference type="OrthoDB" id="73846at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008677">
        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003710">
        <property type="entry name" value="ApbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050838">
        <property type="entry name" value="Ketopantoate_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00745">
        <property type="entry name" value="apbA_panE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43765:SF2">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43765">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase N-terminal" evidence="6">
        <location>
            <begin position="7"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="7">
        <location>
            <begin position="209"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="351" mass="39100" checksum="94EDFC01E6CECFD4" modified="2017-01-18" version="1">MTKSTNVYILGAGSMGGLVACELSSLANCPNITLLLRNPARVEHFRSINKSTIYINRLFKDPVEKISNKFNSASSKDINEDIENLIITTKTYQTQNALKDYLPFIKPTSNILLIQNGLGVVDELYKDVWPNIGERPNIYQGVISHGGFITSNTGDNYEISHAGFGDLKIAKFPRDPNSAEEEEIVKPDFLKQLEDAKGLATSVLSYSDLLLIQLKKFVVNACMNPVTAIINCVNKELKSFDDLSKLFMDITNEVIDVFMATNPLLSKNPNASKILDKQSLVDYVVLCGTVWNAQNSSSMRQDTLHLRDTEVDYINGYVVKVAEANGLKADVNKTITNLVKLRLSVNRIREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1N9</accession>
    <name>A0A1E3P1N9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hsp70 nucleotide exchange factor FES1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Hsp70 nucleotide exchange factor fes1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_63896</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FES1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038606.1">
        <property type="nucleotide sequence ID" value="XM_019183631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1N9"/>
    <dbReference type="STRING" id="683960.A0A1E3P1N9"/>
    <dbReference type="GeneID" id="30200877"/>
    <dbReference type="OrthoDB" id="10250458at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071629">
        <property type="term" value="P:cytoplasm protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050693">
        <property type="entry name" value="Hsp70_NEF-Inhibitors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013918">
        <property type="entry name" value="Nucleotide_exch_fac_Fes1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316:SF18">
        <property type="entry name" value="HSP70-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316">
        <property type="entry name" value="PROTEIN FOLDING REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08609">
        <property type="entry name" value="Fes1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nucleotide exchange factor Fes1" evidence="5">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="283" mass="32141" checksum="B6CA621A510DE23B" modified="2017-01-18" version="1">MDKLLNWSLAQQDPETAKKVGAPDPKLLAELFGQNVDDATLMKQNIELIENPECDEDNKLTAFDNFEMLIENLDNANNIENLKLWPKLINLLDSENDEFKNLTSSIIGTSVQNNEKSQTDFLKYPQGFTKLISLAKSNKVVRIKALYALSNLIRNNEQSYELFDQHKGWELLGPIITDKEAKDKVKLRSLSLLGAILSIAPKDDIKKHIHEYSIIEKILSLIKPGANISVVDKSLNLIVHLINGGYEFNDDEVKQVKESVEIIEKDFHDVINIDDFQVLKQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1V4</accession>
    <name>A0A1E3P1V4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome b-c1 complex subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_85018</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the UQCRB/QCR7 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038673.1">
        <property type="nucleotide sequence ID" value="XM_019185826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1V4"/>
    <dbReference type="STRING" id="683960.A0A1E3P1V4"/>
    <dbReference type="GeneID" id="30203072"/>
    <dbReference type="OrthoDB" id="425749at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099617">
        <property type="term" value="C:matrix side of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1090.10:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1090.10">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003197">
        <property type="entry name" value="QCR7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036544">
        <property type="entry name" value="QCR7_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12022:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12022">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 14 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02271">
        <property type="entry name" value="UCR_14kD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000022">
        <property type="entry name" value="Bc1_14K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81524">
        <property type="entry name" value="14 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="125" mass="14352" checksum="CC54B92F65F4F6B1" modified="2017-01-18" version="1">MASFTSIAKASDFILRTPVLKSIFVPASKFFCQYAGYRQMGLKLDDLIHDEHPIVQKALTRLPKEEIYARNFRTLTAAQCGITHHLLPKNEQVKAEDDVPYLLPYLLEIEAEAFEREELDNIKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1Z5</accession>
    <name>A0A1E3P1Z5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_94873</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <location>
            <begin position="165"/>
            <end position="183"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="349" mass="39554" checksum="F5123F719B590F53" modified="2017-01-18" version="1">MVGNILRFTRHFSSFSALKRHHDFKEFETMFKQRFTPKKGVTIKEATLIESIHDIIKFRSHCKKIDAPSVDGLFNEIVEAPNFRVDEKGLKKLLLMKLPIENTLSLIKSYNVKNPSSVISKETAMIPLRKCLWDGDVDGAIKIVDLTTGSDKYISKKKFDHGKDLFKYFGGFVGSIGVVDFGLRSVGVEPMMGMYAMFATYVLNMTFFATMAFSGKIAGKSDNLQFVQGIPQTYRVLHSDELEMLSRTAEVDAIVNGVDNFASAEFVEKISGKNLELIEPESQILLQEYWLSGGDNFEWVEPDQDPAELLWKQRLQKLNPKTIKADTKWTEKLIEAKRDGNSEIKNSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P210</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_63728</name>
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    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038441.1">
        <property type="nucleotide sequence ID" value="XM_019183599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P210"/>
    <dbReference type="GeneID" id="30200845"/>
    <dbReference type="OrthoDB" id="10523105at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="70" mass="7337" checksum="FBE07F1390739A5C" modified="2017-01-18" version="1">MFPTAIRAFSRSHVAMNASAPTGKKAAVQWVVYFIGITAGLTAGAAVLLNDEAHKNKQVVEAAKPEPTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P248</accession>
    <name>A0A1E3P248_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
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    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_63917</name>
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    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038630.1">
        <property type="nucleotide sequence ID" value="XM_019183638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P248"/>
    <dbReference type="STRING" id="683960.A0A1E3P248"/>
    <dbReference type="GeneID" id="30200884"/>
    <dbReference type="OrthoDB" id="3979937at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021013">
        <property type="entry name" value="ATPase_Vma12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31394">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31394:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11712">
        <property type="entry name" value="Vma12"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="146" mass="17155" checksum="8DDF4FBDA68AD725" modified="2017-01-18" version="1">MTKFIIPNELIPRLDTIQHGSTIRQRGWITTNDLIYISQVYKIELSQLTKNLQIYISPPPKSSYTPEFAAQLAKLKIIQQEQEYQELINRGNYKFDKDYISPSEFAKEVKNQITTIVNILVSVLSVVYAIWYWTDNYQFNQGTRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P290</accession>
    <name>A0A1E3P290_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Glutamine synthetase</fullName>
            <ecNumber evidence="3 11">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_78243</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 11">
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9 10">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="3 11"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038803.1">
        <property type="nucleotide sequence ID" value="XM_019184688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P290"/>
    <dbReference type="STRING" id="683960.A0A1E3P290"/>
    <dbReference type="GeneID" id="30201934"/>
    <dbReference type="OrthoDB" id="1936100at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.70:FF:000004">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000004">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050292">
        <property type="entry name" value="Glutamine_Synthetase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852:SF57">
        <property type="entry name" value="GLUTAMINE SYNTHETASE 2 CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="12">
        <location>
            <begin position="23"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="13">
        <location>
            <begin position="109"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ59596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="371" mass="41684" checksum="C755AB45D4629A96" modified="2017-01-18" version="1">MSETTENTATLQKYLTLDQRGKILAEYIWIDGSGSIRSKGRTLNKKISSVDDLPEWNFDGSSTNQAPGHDSDVYLRPVAYYPDPFRRGDNVIVLAECWNNDGTPNKFNHRHECAKLMAAHKSQKVWFGLEQEYTLFDQYDNVYGWPKGGFPAPQGPYYCGVGSGKVYARDVIEAHYRACLYAGIEISGINAEVMPSQWEFQVGPCEGIEMGDQLWVARYLLHRVAEEFGVKISFHPKPLKGDWNGAGCHTNVSTESMRQPGGIKYIDDAIEKLAKRHKEHILLYGSDNDQRLTGRHETASMTSFSSGVANRGASIRIPRSVAKEGYGYFEDRRPASNIDPYLVTGIIVETIIGEIADADMSKEFQRELSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2D3</accession>
    <name>A0A1E3P2D3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GABA-specific permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_31937</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid/choline transporter (ACT) (TC 2.A.3.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038710.1">
        <property type="nucleotide sequence ID" value="XM_019181900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2D3"/>
    <dbReference type="STRING" id="683960.A0A1E3P2D3"/>
    <dbReference type="GeneID" id="30199146"/>
    <dbReference type="OrthoDB" id="4476201at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015101">
        <property type="term" value="F:organic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000046">
        <property type="entry name" value="Amino-acid permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF6">
        <property type="entry name" value="GABA-SPECIFIC PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="291"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="416"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="454"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="492"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="556" mass="59778" checksum="A567A18363DCA2AF" modified="2017-01-18" version="1">MNKIELTHVTSREVSSHDPSTSISHRIDARHVTDPDELLAEIGYKPELKRTFGTLEVFGIAFSIMGLLPSIATVMSMGLTGGPVTLLWGWFIAGFLILTMGIAMSEMASSIPTSGGLYYWTHYFAPPSLKNTLSFIIGTSNSLSLTGGVCSITYGFAQQVLAAAYLGSNGSFKITNAMTFGVFAAGIIAEFLITCFASGAISRLQTTSIVANVILIVIFFIALPIGTAKNASFNDASFIFGKFENSSTWPSGWAFIQFGFGGCAWVISGFDSCIHMAEEAKNPSKSIPYGIIGSITVCWITGFFIMIVIGACITPDIASILQSSTGQPLAQIFYDSLGQKWAVAFISLSAICQFFMAASCMTASSRQIWAFARDDGLPFSSFIKVVNKKFAVPIRAVVASAIAAATMGLLILAGPIAANALFSISIIGNYVSWSTPQVLRLFFSKNLFKPGPFYLGKVFSPIVVSVSILAQYLILVFSFFPTTSHVSDAKTMNYAIVVNVGAWSLALIYYFSLKRNIYIGPKSNLPDEEFIEGIDYDVHGGKDSGDQISYGKGNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2J5</accession>
    <name>A0A1E3P2J5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glutathione synthase</fullName>
            <ecNumber evidence="4">6.3.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Glutathione synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_91531</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic GSH synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.3" evidence="4"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038913.1">
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        <property type="reaction ID" value="UER00210"/>
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        <property type="project" value="EnsemblFungi"/>
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    </dbReference>
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        <property type="entry name" value="GLUTATHIONE SYNTHETASE"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="13">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="144"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="230"/>
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        <ligand>
            <name>substrate</name>
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        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004965"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00010385"/>
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        <source>
            <dbReference type="EMBL" id="ODQ59706.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
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    </evidence>
    <sequence length="379" mass="42924" checksum="99CF899939C56859" modified="2017-01-18" version="1" fragment="single">MATAAFPELDSAKESILVENLLQWSLANGLVIYPPDFKSYQPVSAPVTLFPTPFPRVAFKDADDVQLLFNELYAKVSSDLEFLSEIIDDLAKFDSGFTGKLWEIFLKAKKIGISQPLSLGIFRSDYMVNEIEGLKPQIKQIEFNTVSVSFGGLSTKVGELHRYLNNHGDYDIGEGTDKYYDEKDLPISKSITKLSEGLADGNYYYNDCVSNTKTVVLVVVQHKERNVFDQRLLEYALLTKHGVKSIRLTLSEIDSKTYIDPQTKKLYIKSTNDEVSVVYFRSGYAPNDFQNEQDWENRLTLETSKAIKAPTLLTQLVGAKKIQQILTDEVILKKFLKEKGESNFQQLLDTFVSIYPLDDSERGKQAKKLAFEKPGNFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2M6</accession>
    <name>A0A1E3P2M6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_78357</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="T_SNARE_dom"/>
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        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="t_SNARE"/>
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        <property type="entry name" value="SNARE fusion complex"/>
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    </dbReference>
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        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
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            <begin position="164"/>
            <end position="226"/>
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            <begin position="114"/>
            <end position="156"/>
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            <begin position="139"/>
            <end position="149"/>
        </location>
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        <source>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ59721.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="254" mass="29673" checksum="08F70FA9425B29C6" modified="2017-01-18" version="1">MSTQLSNLRLIESYLDDLRSLIEESDRIQSLGVSSEDNDYDLRKLLSKITKFLERNDDGDDYDELVEQYQELLNEVKTIDTKDYEYVRNIHIKPLNNKKSVRFNDKVEEASPKKFKPYKDQVLDPPAQSDTDQLFDGRELDDDQEDDTDSVNSSSNQEMFIQHQQELVQQDEHLDTLAQSVRRQRALGLDINQELDDQNILLDDLEAQLDHNDRNLSSGQKRLKYFSEKAKENGQWITIIILVIILVLLLIILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2W1</accession>
    <name>A0A1E3P2W1_WICAA</name>
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        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_78322</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
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        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038890.1">
        <property type="nucleotide sequence ID" value="XM_019184701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2W1"/>
    <dbReference type="STRING" id="683960.A0A1E3P2W1"/>
    <dbReference type="GeneID" id="30201947"/>
    <dbReference type="OrthoDB" id="10264220at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071469">
        <property type="term" value="P:cellular response to alkaline pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006797">
        <property type="term" value="P:polyphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903778">
        <property type="term" value="P:protein localization to vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="397"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="442"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="518"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="551"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="612"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="709"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="738"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="61"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="804" mass="92107" checksum="0A989F402ECB1E54" modified="2017-01-18" version="1">MGQEEAIFRAADMSLVQLYIPSEISRDAVYALGESGTIQFRDLNSKVNTFQRSFVQEIRRLDNVERQYRFFKNELDDREIELKPAPYIEDEQFSPPSKPSDIDEKVENAELLEQRLNQLVDSSETLETRKKDLQQLRHVLKGADEFFSKASKNADLVSLHEDSQDVENQVQQQQQIPNQSFVTGVIPRSKINLLEKILWRVLRGNLFLDSIEIEEPIYDPKTKKTLLRMFSLIKKIAESLDADIYEIESEESKRQALLDEVNGNYSDLTTVLETTNRTLDTELIAIAQELSNWSAIITKEKSVYETMNLFDYDVNRKALIAEGWIPTNEIEQVKQILQTSSTGLQSIVNTLHTTKTPPTYHKTNKFTDAFQNICDAYGVAQYKEVNPGLPTIVTFPFMFAIMFGDMGHGFILGLAALMLVIHEDKIARIKRDEIFDMAYSGRYILLLMGCFSMYTGFLYNDLFSKSMTLFKSGWEWPKHHEGETIFASKTGIYPIGIDPAWHGTENALLFTNSYKMKLSILMGFIHMTYSYCFSLVNHLHFSSMIDIIGNFIPGLLFMQSIFGYLSICIVYKWSIDWNSIGKPAPGLLNMLISMFLSPGKVEEYLYSGQEKIQVALLLIALVCVPWLLLLKPMYFKYKMNQEQKYQELNDDVEAIQDLPTEPDQLAEHQDDDDEEHEGHNFGDIMIHQVIHTIEFCLNSVSHTASYLRLWALSLAHAQLSTVLWSMTIQGAFKTTGFIGSLSIVFLFGMWFILTVVILVIMEGTSAMLHSLRLHWVESMSKFFVGGGKPYEPYSFKTILSSVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P312</accession>
    <name>A0A1E3P312_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secondary thiamine-phosphate synthase enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_91734</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0047 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039012.1">
        <property type="nucleotide sequence ID" value="XM_019186313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P312"/>
    <dbReference type="STRING" id="683960.A0A1E3P312"/>
    <dbReference type="GeneID" id="30203559"/>
    <dbReference type="OrthoDB" id="10255963at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047_YjbQ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00149">
        <property type="entry name" value="TIGR00149_YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJBQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615:SF8">
        <property type="entry name" value="UPF0047 PROTEIN C4A8.02C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
        <property type="entry name" value="YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004681">
        <property type="entry name" value="UCP004681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111038">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="16190" checksum="9447DB870CAF937D" modified="2017-01-18" version="1">MSWYQKKISLKSRSRGAYLITEEIINALPELKDYKVGVLHLFLQHTSAGLSLNENWDSDVRQDLNLALNELVPDDVKGTHYAHSSAGEGPDDMPGHIKSTLVGTDLTIPISNGRLATGTWQGIYLLEFRDYQHTRNVVATINGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P322</accession>
    <name>A0A1E3P322_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CoA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_91747</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039022.1">
        <property type="nucleotide sequence ID" value="XM_019186317.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P322"/>
    <dbReference type="STRING" id="683960.A0A1E3P322"/>
    <dbReference type="GeneID" id="30203563"/>
    <dbReference type="OrthoDB" id="5138418at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303:SF2">
        <property type="entry name" value="COA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303">
        <property type="entry name" value="CYTOPLASMIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA-binding" evidence="1">
        <location>
            <begin position="11"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="118" mass="13179" checksum="93CE7766B8F0697B" modified="2017-01-18" version="1" fragment="single">MSMKSFFSPSRVYAVIGATPNEQKFGFKVFNWYLARDLPVIPIHPSATHVLHVPTSQDIALAINRADEEYPNNDGISISFITPPKVTESVLSELEAKNLVNKVKGVWYQPGAYDDEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P353</accession>
    <name>A0A1E3P353_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Letm1 RBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_15260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038887.1">
        <property type="nucleotide sequence ID" value="XM_019180638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P353"/>
    <dbReference type="STRING" id="683960.A0A1E3P353"/>
    <dbReference type="GeneID" id="30197884"/>
    <dbReference type="OrthoDB" id="73691at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="33993" checksum="392E600341247291" modified="2017-01-18" version="1" fragment="single">ANNTLPIVLRTKKVVFEDIQKENPKSEGVQLKFKQYTGYFKSIIHFYRIGIQNVWQNRTRVAEIKSKYSIENVEQDGSITKRKLKNSGDLINLLNALETMQIIEQETLKKFDKTTILNLNRLEFQTILRTQQDFYKIPLFAMILLVFAETTPILCYIFPELAPSTCVFPGLLIKKYSSSTKAFQQLTKLRLERYGAVYSQGEIPFQSVYKLPHDELKLLVQSLNLKSKYLPVFLYPISTLQARLKFHYDLIKVDNHYLINGEDGNNIWGLNKNELIRSCLDRGLLDLEKDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3C7</accession>
    <name>A0A1E3P3C7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_68538</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039186.1">
        <property type="nucleotide sequence ID" value="XM_019184123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3C7"/>
    <dbReference type="STRING" id="683960.A0A1E3P3C7"/>
    <dbReference type="GeneID" id="30201369"/>
    <dbReference type="OrthoDB" id="10264505at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051063">
        <property type="entry name" value="PDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672:SF3">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133645" description="Thioredoxin domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="12"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ59979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="306" mass="35459" checksum="234317A45E4AEAA8" modified="2017-01-18" version="1">MLNIFKYCLFLLLINSSFAYKTFKDVIVADDSNFEDLIHEKGKYTFINFYSKQCIHCQKLAPNFQILTQLFNNTKIQIAQLEGRENKRIRKKESIAGFPTLRLYAFDGTHLGSYNGDRSIEDMADFVNSHTGVVPNWPDLKVLQISSKLELDEIVTMEDNVVLAFIAPWLENWVGKYSFIERLASKLSNVRFIKVDATSEEATELTSLYKIDKFPTILHFNSHDSLTNFRILEINKDIDIIDGPTIEEFLNNQIGQAYDSIENLEIVKGEDAEQQDVQLKRGFNFDVQKKTTPEQEEEAYKRLREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3K2</accession>
    <name>A0A1E3P3K2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acetyl-CoA hydrolase</fullName>
            <ecNumber evidence="4">3.1.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Acetyl-CoA deacylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_56232</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + H2O = acetate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="3.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyl-CoA hydrolase/transferase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.1" evidence="4"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038689.1">
        <property type="nucleotide sequence ID" value="XM_019182776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3K2"/>
    <dbReference type="STRING" id="683960.A0A1E3P3K2"/>
    <dbReference type="GeneID" id="30200022"/>
    <dbReference type="OrthoDB" id="10250396at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008775">
        <property type="term" value="F:acetate CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003986">
        <property type="term" value="F:acetyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006083">
        <property type="term" value="P:acetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.70:FF:000002">
        <property type="entry name" value="Acetyl-CoA hydrolase Ach1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.10:FF:000003">
        <property type="entry name" value="Acetyl-coA hydrolase Ach1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.20:FF:000001">
        <property type="entry name" value="Acetyl-CoA hydrolase Ach1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.70">
        <property type="entry name" value="4-hydroxybutyrate coenzyme like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.20">
        <property type="entry name" value="Acetyl-CoA hydrolase/transferase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.10">
        <property type="entry name" value="Glutaconate Coenzyme A-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026888">
        <property type="entry name" value="AcetylCoA_hyd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038460">
        <property type="entry name" value="AcetylCoA_hyd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046433">
        <property type="entry name" value="ActCoA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003702">
        <property type="entry name" value="ActCoA_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43609">
        <property type="entry name" value="ACETYL-COA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43609:SF1">
        <property type="entry name" value="ACETYL-COA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13336">
        <property type="entry name" value="AcetylCoA_hyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02550">
        <property type="entry name" value="AcetylCoA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-CoA hydrolase/transferase N-terminal" evidence="9">
        <location>
            <begin position="10"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Acetyl-CoA hydrolase/transferase C-terminal" evidence="10">
        <location>
            <begin position="328"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="524" mass="58134" checksum="92A1CA40281B8A9C" modified="2017-01-18" version="1">MSQILKQRVRYAPYLQKLRTPEQCVELFKNGQYLGWSGFTGVGAPKVVPDALAQHVEDNNLQGQLQFNLFVGASAGPEESRWATNNMIARRAPHQVGRPIASNINDGKTQFFDKHLSMFPQDLTYGFYTREKENDLLDFTIIEATAITEDGSIVPGPAVGGSPEMLSVSDKIIIEVNTVTPSFEGLHDIDLPINPPNRPPLPYTSADHKNGRISIPVDPNRVAAIVESTKKDVVPPNTPSDQMSKNIASHLVKFFRDEVKAGRVPENLHPLQSGIGNIANAVIEGLADANFKHLTVWTEVLQDSFLELFQNGSLDYATATSIRLTEKGFEKFFDNWDEFSSKLCLRSQVVSNSPEVIRRLGVIAMNTPVEVDIYAHANSTNVSGSKMLHGIGGSGDFLRNAKISIMHTPAARPSKKDPTGISCVVPFASHIDQTEHDLDVIVTDKGLADLRGLSPRERSRVIIKNCAHADYVDQLLDYVNRAEFYAQKTKSLHEPHILRDAFKMHLNLAQNGTMKLDSWDQKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3P5</accession>
    <name>A0A1E3P3P5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_19617</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039242.1">
        <property type="nucleotide sequence ID" value="XM_019180833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3P5"/>
    <dbReference type="STRING" id="683960.A0A1E3P3P5"/>
    <dbReference type="GeneID" id="30198079"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045719">
        <property type="term" value="P:negative regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060917">
        <property type="term" value="P:regulation of (1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14004">
        <property type="entry name" value="STKc_PASK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000320">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000314">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF51">
        <property type="entry name" value="PAS DOMAIN-CONTAINING SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="766"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="635"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="670"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="218"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="670"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="693"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1024"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ60035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1024" mass="115397" checksum="B14CF253F68F0A36" modified="2017-01-18" version="1" fragment="single">NLNEILKFPKESTHAYSYAHLSPNSLAVRLSILKRSLEILVDHPDLLQKEDSDLLPDGGAGISEALHLDIENFISQIQNEDDEKFKIQSNASSAALAALFTTQNNSSGTSTPRKPPSKDSTTINTTHLGLKSLINILKEDKSLKSGESQKELAMNLHDLSLSNVADEAMSQPMVRSDSESQLKIKLMHALATPFYEPIVSTPIISLQKGASTLALSSLNQQQQQQQSSAPGISGDNRIFHSMSQKNTSPQAVFTCELNDPWNLKAANDLACLIFGVSRNSLKRLTLLDLISPDSRDFVLNRLVKNSIERNQEIIFSGQVIAIAKPGNKLTWASMWAKKKGNLIICMFDQVPCDSIDLVACLDSLKIEFFKKISGNSFFNNTFLSSLDTLSQLLNNIEEYTSLEDENDGNYQNIPEVYLQSQSINVQRYFTLKKNGFNIPCALFTKIIDDNLNKVKLKIHSLPYIAGVFIISNNPTNYNILSFNESIAKNLFGFHESQLKHQSITKIIPNFQKIMTIILQNFKNLKIIPGLVLPEHFFRQMNSILEKSGNGSTTNDLEANFLNSIGIDGKHVDGSTIKIDVQLRCSNVDTLILWVTHSRSVYQNELDSKVSTARPTMKKTRSVRSVRIVDNKEEIVEEEVEVEHDGSKDDEDEDEIPSQLSILNENEIQSVSRSSSLRSSNVTSSSDLSTLSSIKNDQFSNDDIGQTSSNDQLNETDLENFKVLSKNMNEASLLKIENDTIEKNKSISKYYPKKIGLKRREKSIKEFKILKNMGQGAYGKVVLAEYRKDPNYRIVIKLIIKERILVDTWVRDRKLGTIPSEIQIMSTLNNNPHPNIMRLIDFFEDDDYYYIETPQHGFPTPGIDLFDLIELKKSMTEFECKSIIKQCISAINHLHNNGIVHRDIKDENLIIDNNGVIKLIDFGSAAYVKTGPFDVFVGTLDYAAPEVLNGLPYEGKPQDVWSLGILLYTLIYKENPFYNVDEIMEGELRIPFVTSQGCIDLIKKILQRDIRKRPKMADIVCDSWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3U3</accession>
    <name>A0A1E3P3U3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Copper-fist domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_78507</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039081.1">
        <property type="nucleotide sequence ID" value="XM_019184737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3U3"/>
    <dbReference type="GeneID" id="30201983"/>
    <dbReference type="OrthoDB" id="3981116at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.430.10">
        <property type="entry name" value="Copper fist DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001083">
        <property type="entry name" value="Cu_fist_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036395">
        <property type="entry name" value="Cu_fist_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00649">
        <property type="entry name" value="Copper-fist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00617">
        <property type="entry name" value="COPPERFIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM01090">
        <property type="entry name" value="Copper-fist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00412">
        <property type="entry name" value="Cu_FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57879">
        <property type="entry name" value="Zinc domain conserved in yeast copper-regulated transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50073">
        <property type="entry name" value="COPPER_FIST_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Copper-fist" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="391" mass="43170" checksum="599AD4D87684862B" modified="2017-01-18" version="1">MILLDHERLGCEQCIRGHRAADCQHLAKYLISIKGRGRPAAKDESIRFRVSPHPRVIKTTWEAIESKNGRGKSGTRKFCYHLDPGTPVELYKVKLGKGFKVIGPVTDFTYHSSFRVKSNADGTTENVGSSSSQMSQARVPQLPYLQPTATSSTVPSFPAHLATQYNGFHRPIPVKQESISPPTVPNPQAAAASQAVALSPSDPNYLVPPTPAPSPVNGMISRESSRVMDEMFEIDSRPSVSSMSSNSTSHSDLVLNSLQQHHQAQQQLQQPLTTEELNILQLNGLFDHEAAFKAFQADNSIQCDQPIHFQQQEQPQYEPQATSSANSSLTFQLDASTSVEPLNGEQTIENETSNNGLLNSEYFFQHFVADSNEPQWPSNFVRGSNDGYSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P450</accession>
    <name>A0A1E3P450_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">asparaginase</fullName>
            <ecNumber evidence="2">3.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_29798</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-asparagine + H2O = L-aspartate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="EC" id="3.5.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the asparaginase 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.1" evidence="2"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039402.1">
        <property type="nucleotide sequence ID" value="XM_019181651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P450"/>
    <dbReference type="STRING" id="683960.A0A1E3P450"/>
    <dbReference type="GeneID" id="30198897"/>
    <dbReference type="OrthoDB" id="542841at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006530">
        <property type="term" value="P:L-asparagine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08964">
        <property type="entry name" value="L-asparaginase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1170:FF:000001">
        <property type="entry name" value="L-asparaginase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004550">
        <property type="entry name" value="AsnASE_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00520">
        <property type="entry name" value="asnASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500176">
        <property type="entry name" value="L_ASNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00057">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="L-asparaginase N-terminal" evidence="9">
        <location>
            <begin position="28"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="10">
        <location>
            <begin position="259"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <feature type="active site" description="O-isoaspartyl threonine intermediate" evidence="5">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="115"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="382" mass="42013" checksum="95DDE63DBE09183B" modified="2017-01-18" version="1">MPNQDISITTKTNNEIDIDSHDSSLPVVKIFGTGGTIASKGSTNTQTAGYQVDLNIEDLINSVPDLAEIAHLKYQQIVNLDSKEINSTHLLLLHREIKKAYEEEGIKSIVITHGTDSLEETAFFLELTINYPDIVIVLTGSMRPHSSISSDGSFNLYQAVAVSANEKSKGRGVLVVLNDSIGSGFYITKSNANSLDTFKSIGQGYLGSFVDNEVHFYYPPSKPFTNSLFFDVDSLKLTELLLFSSNPTSSTRRHEFPEVTILYNYQGFNPRIIELAVQQMDSKALVFACSGAGSLTDETNEILKWIWHKYNIPVVYSKRSMDGTVPRAALPKNDNGAVYEGAIAGGYLNPQKCRILLQLALQKGYTVSKIKSIFENGGIYGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4B1</accession>
    <name>A0A1E3P4B1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_54064</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCM10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039472.1">
        <property type="nucleotide sequence ID" value="XM_019182730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4B1"/>
    <dbReference type="STRING" id="683960.A0A1E3P4B1"/>
    <dbReference type="GeneID" id="30199976"/>
    <dbReference type="OrthoDB" id="273123at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040184">
        <property type="entry name" value="Mcm10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055065">
        <property type="entry name" value="OB_MCM10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015408">
        <property type="entry name" value="Znf_Mcm10/DnaG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13454">
        <property type="entry name" value="PROTEIN MCM10 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13454:SF11">
        <property type="entry name" value="PROTEIN MCM10 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22379">
        <property type="entry name" value="OB_MCM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09329">
        <property type="entry name" value="zf-primase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MCM10 OB-fold" evidence="10">
        <location>
            <begin position="153"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger Mcm10/DnaG-type" evidence="9">
        <location>
            <begin position="291"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="54"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="537"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="537"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="568"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="600" mass="68915" checksum="AF6E5D3B29B51852" modified="2017-01-18" version="1">MEDPREIQSDDEFSKLTDEERIEPEDSGDESVDEELQKLERRKLELLEKKKRKVELQARKKATAEVSRSPSPVKQKRSNIEDVKPKNLTRDLTHSNDTDQKGSTSYFIKMLDESQDHDQNEKDEARKLLSSRIYTFDEDLVCEPFAVNEKEYFSALNVSKRYLTHEALSSILEEKKILRLPKLYAKVAPPTFDEPRYANWVVIGIISKKSSPRATSDNRSKFMTIALTDFQLTVDILIFGKNVEKYVKLRVGDIVAILNPEIYIWKPDSQGVIRSFSLSIKHSYDCVLEIGKARDFGLCQSMKRDGTICGTPINTSKEDCCFYHKELRIRKTQGKRVELNGSFSLRSPTKNGRKQQFLMGGKDNKGNYSNSTIVEDRYAPKVDRISKSLVYFSNPHAGKAFFNDSYQNPDILKNIEEKKRKIQERKKESALRAKLLRLGDKPIEKKAPISKEEQIALQKKKALLKTAFQKDTLKNIGYDPTSKPNAKGVLDSDEVGDANETVLNEFSSLTQNKNINLGLSKEELAKRKEQRVQADSLMKRLKDREEKMKRESLQETFTKKRKGKDVNLDSESDSDFEVEGGDTKDAYIKFKLSQSKSQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4S7</accession>
    <name>A0A1E3P4S7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_63051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039706.1">
        <property type="nucleotide sequence ID" value="XM_019183484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4S7"/>
    <dbReference type="GeneID" id="30200730"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="164" mass="18762" checksum="5357B3E4745C41EF" modified="2017-01-18" version="1">MANLTHFDQLDTSPYTNRVDFEHELDSDITIRGTENIENIKYSTEYSRDHLMTSPLVSQTRKHRPVRFADSSPRAHFSTSNIQNLIDLDDYYEDEDDEDYYPTSSDADETDDDFPIDGLEESHGLIQRIQPDVTTMEKVVILSTFIGTALGIVVGYVLFLSESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4U0</accession>
    <name>A0A1E3P4U0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Intradiol ring-cleavage dioxygenases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_83898</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the intradiol ring-cleavage dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039074.1">
        <property type="nucleotide sequence ID" value="XM_019185616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4U0"/>
    <dbReference type="STRING" id="683960.A0A1E3P4U0"/>
    <dbReference type="GeneID" id="30202862"/>
    <dbReference type="OrthoDB" id="5238185at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018576">
        <property type="term" value="F:catechol 1,2-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009712">
        <property type="term" value="P:catechol-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.130.10">
        <property type="entry name" value="Aromatic compound dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007535">
        <property type="entry name" value="Catechol_dOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000627">
        <property type="entry name" value="Intradiol_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015889">
        <property type="entry name" value="Intradiol_dOase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050770">
        <property type="entry name" value="Intradiol_RC_Dioxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33711">
        <property type="entry name" value="DIOXYGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G02910)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33711:SF7">
        <property type="entry name" value="INTRADIOL RING-CLEAVAGE DIOXYGENASES DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00775">
        <property type="entry name" value="Dioxygenase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04444">
        <property type="entry name" value="Dioxygenase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49482">
        <property type="entry name" value="Aromatic compound dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00083">
        <property type="entry name" value="INTRADIOL_DIOXYGENAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Intradiol ring-cleavage dioxygenases" evidence="7">
        <location>
            <begin position="126"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="318" mass="35603" checksum="70BF85624B1F515E" modified="2017-01-18" version="1">MSDETITENTKIVNSKTPNPRLKFILSKLVDHLHDFARGTRLTTEEWDVGIDFLTPVGQTCTEIRQEFILLSDVLGLSVLVDGISHPKPEGATIGTLLGPFHTHDAEEHQQGDSICSEGKGEPLLITGKLTDLQGEPIEGASIDLWECDENGLYDTQYPDRDGPDCRGIVHTNAQGEFVIKCIKPTPYPIPHDGPVGQLLQVIKRHPYRPAHIHFIIEKPGYDKLITALYHKGDRFENSDAVFGVKTPLLFELVPLGEKLAKKYDMNANDWYLEQNFTIVTEGEARECRIAKSKKFLKENVEGEFEFNENGLPIASVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4W1</accession>
    <name>A0A1E3P4W1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_92819</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal/bacterial/fungal opsin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039610.1">
        <property type="nucleotide sequence ID" value="XM_019186380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4W1"/>
    <dbReference type="GeneID" id="30203626"/>
    <dbReference type="OrthoDB" id="536545at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15239">
        <property type="entry name" value="7tm_YRO2_fungal-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000160">
        <property type="entry name" value="Related to Opsin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001425">
        <property type="entry name" value="Arc/bac/fun_rhodopsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043476">
        <property type="entry name" value="Yro2-like_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28286">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28286:SF1">
        <property type="entry name" value="30 KDA HEAT SHOCK PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01036">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00251">
        <property type="entry name" value="BACTRLOPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01021">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="314" mass="34803" checksum="F82229833AB5199C" modified="2017-01-18" version="1">MAYIDLELVKRGGNRAIEINPPTGADFHITEHGSAWLWSAFCVFIVIGLVIIGLMFRKPANERVFYFTSILPTMFMALAYFTMASDLGWAPIRAEFNHVKTDTQDEFPGYRQVFYAKYIGWFLSFPFHVINLGFLSRTPWGQIAFNVCFTEMYVVMLLIASLVSSTYKWGFYVLAIGALIFVTVSTFVTSGRLADKVGKDCHAYFHIAAGILTVLWFIYPICFGVSEGGNDLQPDSETIFYGVLDVVVFGFYSLAFLLATNDFTLERLGLLTPEFEVIPHEKSGLSVAGRASGETAVSRSASGVTSPSAASPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4W2</accession>
    <name>A0A1E3P4W2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like modifier HUB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_30820</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039662.1">
        <property type="nucleotide sequence ID" value="XM_019181766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4W2"/>
    <dbReference type="STRING" id="683960.A0A1E3P4W2"/>
    <dbReference type="GeneID" id="30199012"/>
    <dbReference type="OrthoDB" id="3881at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000753">
        <property type="term" value="P:cell morphogenesis involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033120">
        <property type="term" value="P:positive regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043687">
        <property type="term" value="P:post-translational protein modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01791">
        <property type="entry name" value="Ubl_UBL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000052">
        <property type="entry name" value="Ubiquitin-like protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039732">
        <property type="entry name" value="Hub1/Ubl5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13042">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="3">
        <location>
            <begin position="2"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="73" mass="8160" checksum="8A31C9799EBAA1F2" modified="2017-01-18" version="1">MIEVVVNDRVGKKVKVKALEDDLVGDFKKLLAAQIGTAWEKILLKKGNTTLKDHISLGDYEIHNGTSVELYYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4Z4</accession>
    <name>A0A1E3P4Z4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vacuolar protein sorting-associated protein 55</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_30225</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OB-RGRP/VPS55 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039124.1">
        <property type="nucleotide sequence ID" value="XM_019181703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4Z4"/>
    <dbReference type="STRING" id="683960.A0A1E3P4Z4"/>
    <dbReference type="GeneID" id="30198949"/>
    <dbReference type="OrthoDB" id="14246at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034424">
        <property type="term" value="C:Vps55/Vps68 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007262">
        <property type="entry name" value="Vps55/LEPROT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12050">
        <property type="entry name" value="LEPTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12050:SF0">
        <property type="entry name" value="RH04491P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04133">
        <property type="entry name" value="Vps55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="14236" checksum="8AFDFE59729740C7" modified="2017-01-18" version="1">MQVAPLTKIIFLAGFLAAGFLLIILSCALWNNWYPLYVVGIFLAAPIPNAIFSRTEFDDFMSDSSSATNDFAKFLTGTLVLSGLALPIVLYHVRLVVLPATVMSMTGGLIVYTSIIVFGMFFHENEEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4Z6</accession>
    <name>A0A1E3P4Z6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_62869</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    </dbReference>
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        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051186">
        <property type="entry name" value="RRM_HNRPC/RALY_subfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968:SF26">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
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        <location>
            <begin position="301"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="230"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="373"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="523"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="605"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="54"/>
            <end position="67"/>
        </location>
    </feature>
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        <location>
            <begin position="73"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="89"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="126"/>
            <end position="141"/>
        </location>
    </feature>
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            <begin position="155"/>
            <end position="167"/>
        </location>
    </feature>
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        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
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        <location>
            <begin position="230"/>
            <end position="244"/>
        </location>
    </feature>
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        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
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            <begin position="394"/>
            <end position="412"/>
        </location>
    </feature>
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        <location>
            <begin position="548"/>
            <end position="564"/>
        </location>
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            <begin position="565"/>
            <end position="575"/>
        </location>
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            <end position="625"/>
        </location>
    </feature>
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            <begin position="643"/>
            <end position="658"/>
        </location>
    </feature>
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        <location>
            <begin position="679"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="700" mass="78075" checksum="A6BF0CA6955ACF36" modified="2017-01-18" version="1">MSDDNVYEFHENEQSTFDIPDDTHTEKEPEEEVVGKEEEDNEEYDPSTVGNDNADSDDPYSSVDEDAEEKKIQSQSNSQEPSNEASEPESVKESSKENIEEKKELEVNQDKEETKEETKEETKEEEKEEKEEEEDDDDYDPTAEVSTNEEESKAQPVSSGISATETNQQSATPTTQATSLASSLPSNSNTNPDIQQSVAKIMSSNVLSDPEFLKLSPTEQQQRILTLLGTNNNTQTQQTQSTSSYSAPAPNYTDFNSQTYPKDHRPDLSLPMTIEEISKYNAFIDGEAKYSSTRPTPAPNSRLFVGNLPANVTSKQDLFRLFNGYGHILQISIKGSYCFIQYSDAEAVERAINAERGIPFNGKDLVLEIAKNSRPGQGHQQTTNDRLYNNNFHNQDRKRGREDHFEGSDDRSTRRRRGGALECQIFVKRTADPQYARDVARQFQSAGIATEYEFLKPGQDLSKKINDVAYNGILSVVLINKNRNSDVQIYEKTPDGSIRYDEYLSVSAEDATQLLLRAKQSRFTGGGPSAAAPRGGYNSNSYGSRNNFSQQFEPSYGQQSNNFYQPPAQQQALPQQQQLLQSLQNLPPSALQNLLAMAQGQQPAAIPQQQFQQPYQQPYQPQPAAGSYGSPQVDLASLLGKVQSQAQPQQQYGNQGYNPLGGNDSYQPTNYQQPPPQQSQPQNSGSNVQSLLDTLAKLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P575</accession>
    <name>A0A1E3P575_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rgp1-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_77291</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019184535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P575"/>
    <dbReference type="STRING" id="683960.A0A1E3P575"/>
    <dbReference type="GeneID" id="30201781"/>
    <dbReference type="OrthoDB" id="1918at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014848">
        <property type="entry name" value="Rgp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12507">
        <property type="entry name" value="REDUCED GROWTH PHENOTYPE 1 RGP1, YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08737">
        <property type="entry name" value="Rgp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="47"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="570" mass="64517" checksum="B549BD47AC42F0D1" modified="2017-01-18" version="1">MLTHAIYNQLLTKNVRIEIVYESSPVFADEEVSAIVRFRHLGEPSKQTIHHNASESNTNGHQEQQQNSAQSHESEPEGGWFGKRISMQLSNATRALFLEEAEKTEKKPALRNRPVDFLSGYVQLSGFFTYDEQVVDGEKLKDFRMKAAVAGRIGGLEGLELSKNLNEGVFGRLTSGIGNLLNTSIDELAAEDQDKKKELEKLVPFFSTSQSLLFSELHLESGEVKTFYFKCKLPKTLPPSYKGSSIGINYHLIVGASLNKSIPKPLNLHFPLKIYPNFDEEGYQPISNLDKFILLPPDKILNEAVQVYGGRRTSFKTIKKVILTEPKLTQNTKKEKFISRIEHLIDNEKDQDLNINDFKGINSKETLTAFTESINNRGEIDENIPVRVQKDGYTFEKQVTKIQTQYIISRNGQSITTLTFSKPFYKIGEEIKLSLDLHDKDLQTTGVLISLESLELIRDSFSADLDMTKTLPRSLAFYKESYPTYNVKNLPVNIPIPLHATPQFKTNIFESRWCLSLKFVLSQDEKYEEVHSDETGSMLNAKENISGTDFICRLPIPVLPSDQDFGGITT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P583</accession>
    <name>A0A1E3P583_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PABS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_39990</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
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    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:spermine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006597">
        <property type="term" value="P:spermine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.140.10:FF:000001">
        <property type="entry name" value="SPE3p Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000013">
        <property type="entry name" value="Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030668">
        <property type="entry name" value="Spermi_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558:SF11">
        <property type="entry name" value="SPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="SPERMIDINE/SPERMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000502">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="5">
        <location>
            <begin position="11"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="297" mass="33549" checksum="DE2B42172B0A6B68" modified="2017-01-18" version="1">MSDLTHPLIQNGWFKEVSDESFPGQALELKVKKILHSAKSEFQDILVFESTDYGNVLVLDGIIQVSERDEFAYQEMITHLPIFAHPNPKSVLVIGGGDGGVLREVLKHEDIEKIVLVEIDETVIELSKKYLKGMSVAFSHPKINVKLCDGFKYLQDLSLSDDKFDIIITDSSDPDGPAAAFFQQQYFKLLHSALNENGIVITQASENVWLKIELLKNLKATISEIFPVVKYAYTTVPTYTSGQLGLMISSKNPNAKLNIPARTVSKEKEEELFKYYNSEIHKASFILPNWAKYYLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5B0</accession>
    <name>A0A1E3P5B0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNase MRP protein 1 RNA binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_18708</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039832.1">
        <property type="nucleotide sequence ID" value="XM_019180803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5B0"/>
    <dbReference type="STRING" id="683960.A0A1E3P5B0"/>
    <dbReference type="GeneID" id="30198049"/>
    <dbReference type="OrthoDB" id="5414547at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000294">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, RNase MRP-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22573">
        <property type="entry name" value="RMP1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047205">
        <property type="entry name" value="RMP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047204">
        <property type="entry name" value="RMP1_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37792">
        <property type="entry name" value="RIBONUCLEASE MRP PROTEIN SUBUNIT RMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37792:SF1">
        <property type="entry name" value="RIBONUCLEASE MRP PROTEIN SUBUNIT RMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20945">
        <property type="entry name" value="RMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="RNase MRP protein 1 RNA binding" evidence="2">
        <location>
            <begin position="19"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="112" mass="13105" checksum="4FB49AEC7E8926E9" modified="2017-01-18" version="1" fragment="single">IMTIIPKESLVALEHEFGIIKLIHHRNKNQHRVATWWKHLNNLKRYLTKVISLIHTYNRNKDDKVRQKLQKVSRHLYFNICKSAFRAFNGVIALGQFITLGLTLVGALGKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5R7</accession>
    <name>A0A1E3P5R7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein SYS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_40262</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039996.1">
        <property type="nucleotide sequence ID" value="XM_019182552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5R7"/>
    <dbReference type="STRING" id="683960.A0A1E3P5R7"/>
    <dbReference type="GeneID" id="30199798"/>
    <dbReference type="OrthoDB" id="542931at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019185">
        <property type="entry name" value="Integral_membrane_SYS1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12952:SF0">
        <property type="entry name" value="PROTEIN SYS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12952">
        <property type="entry name" value="SYS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09801">
        <property type="entry name" value="SYS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ60789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="22831" checksum="ABE83625AA7C71BE" modified="2017-01-18" version="1">MNNLRHALSGLQRFQGYLKLPNLSEITSQDSSSSSSSSPSKLTIQIILLQCFYYITATVLSFSIAWILGLDFNFGWVFNWDLVSLDNSLGLTIIGMWLFDSLLCVLFMTVIVGRSKLAWDFAITIHAINLVVVWIYSGEFPRSFVWWSVQVGSCLILVSLGTWSTRWKELRETFFEGIVDVEMGHSGQRSAIEMRDVSEDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P609</accession>
    <name>A0A1E3P609_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lipid droplet-associated hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_77387</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. LDAH family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039917.1">
        <property type="nucleotide sequence ID" value="XM_019184548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P609"/>
    <dbReference type="GeneID" id="30201794"/>
    <dbReference type="OrthoDB" id="448051at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019363">
        <property type="entry name" value="LDAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13390">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13390:SF0">
        <property type="entry name" value="LIPID DROPLET-ASSOCIATED HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10230">
        <property type="entry name" value="LIDHydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="35114" checksum="611F923CE5CBA437" modified="2017-01-18" version="1">MLKSFAGVKLPTSVYHQLPRARPANGPLLIFIPGNPGVISYYVPYFKLLGAQFKTLEILGIAQAGHQIPDGFNGDEFDLVVDLQGQIDHKVAVIEEFLSDGAGANRDVLIMGHSVGSWMVERIVLKLLDKVNFKFIGFITPTIIDIHKSEKGGTAYPLIKILPYLNILLAKLSSLLGYLPERITRFLLSQTLRNPPAHALESTSLFISNPQHIRQSIGLAIEELNVIQNDWDFFENEFLKQTTTIPKWFFFSNVDHWVTNETQKEITERMEGVENAFVENSDEMVHSFCVKQSEEFAEATTKALNELYFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P667</accession>
    <name>A0A1E3P667_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Acyl carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_20217</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039649.1">
        <property type="nucleotide sequence ID" value="XM_019180862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P667"/>
    <dbReference type="STRING" id="683960.A0A1E3P667"/>
    <dbReference type="GeneID" id="30198108"/>
    <dbReference type="OrthoDB" id="448946at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099128">
        <property type="term" value="C:mitochondrial [2Fe-2S] assembly complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000035">
        <property type="term" value="F:acyl binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.10:FF:000003">
        <property type="entry name" value="Acyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01217">
        <property type="entry name" value="Acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00517">
        <property type="entry name" value="acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002148">
        <property type="entry name" value="PRK00982.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="ACYL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863:SF28">
        <property type="entry name" value="ACYL CARRIER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13 14" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 14" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Carrier" evidence="15">
        <location>
            <begin position="14"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ60442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="92" mass="10375" checksum="4BAE9368A47CFC44" modified="2017-01-18" version="1" fragment="single">GVRFYSDVPELTKDVIQERIYEILENFDKVSANSKIENSSSLTKDLGLDSLDLVDIIMALEEEFCIEIPEKDQSQFKTVGDAIEYIANTPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P689</accession>
    <name>A0A1E3P689_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VPS4-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_83134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040017.1">
        <property type="nucleotide sequence ID" value="XM_019185497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P689"/>
    <dbReference type="STRING" id="683960.A0A1E3P689"/>
    <dbReference type="GeneID" id="30202743"/>
    <dbReference type="OrthoDB" id="2158714at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013640">
        <property type="entry name" value="Vfa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28218">
        <property type="entry name" value="VPS4-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28218:SF1">
        <property type="entry name" value="VPS4-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08432">
        <property type="entry name" value="Vfa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="194" mass="22436" checksum="2109D8D04A372295" modified="2017-01-18" version="1">MEPFKNIYHLRKVAQSNSKPCAFCYKITGSVLITSDGSSDFFYTCDSHLKDAAFATPIYDKEYNEAKAKKEALDKEIKELKLKWDEKNRYAGWDKLVSKFSSTKKDGKDDDKKDEKDSTSKSEETQIKELSKQVSNHESTLQKQPRTYQLNKDIFKIRLEKKYKSKQSVQSTQLNQKLKDPTFFPSVPQAPPNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6J9</accession>
    <name>A0A1E3P6J9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1</fullName>
            <shortName evidence="8">Oligosaccharyl transferase subunit WBP1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_28654</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DDOST 48 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039779.1">
        <property type="nucleotide sequence ID" value="XM_019181521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6J9"/>
    <dbReference type="STRING" id="683960.A0A1E3P6J9"/>
    <dbReference type="GeneID" id="30198767"/>
    <dbReference type="OrthoDB" id="29105at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004576">
        <property type="term" value="F:oligosaccharyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005013">
        <property type="entry name" value="DDOST_48_kDa_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055459">
        <property type="entry name" value="OST48_MD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055457">
        <property type="entry name" value="OST48_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10830">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10830:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23358">
        <property type="entry name" value="OST48_MD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03345">
        <property type="entry name" value="OST48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009027338" description="Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1" evidence="8">
        <location>
            <begin position="19"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="393"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="OST48 N-terminal" evidence="9">
        <location>
            <begin position="26"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="OST48 middle" evidence="10">
        <location>
            <begin position="276"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="428" mass="48955" checksum="735FA9DEB690F602" modified="2017-01-18" version="1">MLYFKGLILLVIATVSLAVSVLDPATLIIYDPKLTNLDDYSKLIDNLKKQSYKLTIKQIDDESIKLYKNDEKLYQNVLIFPTKTRTIGSDITGFKLLEFFNDGGDVFIISNPNGLSESVRTFINQLGIFPSPRNHQLLDHFVYDETKAFESHDVLKLNESNIVDQGAIIKNKVDSFNYKGSSALLGNGRLLIPVVQAPTTSFVKDLKNDDFVLENSWTVGTQGYLAVGFQGMNNARAFWIGDEDLLNEDFAQDFIKWTFQETGVLKVTSVEHSHLDGRSYEETPYKIKDDVIYKIGVSEWDGSKWVPFITDDLQVEIKLLDPYHRLNLKPILTQGESTIYGVEFKLPDHHGVFSFHTNYRRSGKSFIDEKNTLAIRHLANDEYPRSWDITNSWVYITSAVVVFIAWVFFVVFFIYSTDETLVEAKKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6K8</accession>
    <name>A0A1E3P6K8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription activator GCR1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_61625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040266.1">
        <property type="nucleotide sequence ID" value="XM_019183218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6K8"/>
    <dbReference type="STRING" id="683960.A0A1E3P6K8"/>
    <dbReference type="GeneID" id="30200464"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060963">
        <property type="term" value="P:positive regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052146">
        <property type="entry name" value="HOT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022210">
        <property type="entry name" value="TF_GCR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784:SF2">
        <property type="entry name" value="HIGH-OSMOLARITY-INDUCED TRANSCRIPTION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784">
        <property type="entry name" value="PROTEIN MSN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12550">
        <property type="entry name" value="GCR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription activator GCR1-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="111" mass="13204" checksum="B11610D986E9F38E" modified="2017-01-18" version="1" fragment="single">LNPNPKSVQEVLDEYYYGYQGQPSLKYLEESQKRWRKGNKNLSKTFSRRFRVVTAVEIGTQMYAAEMGGNEALAKERVVNELENLRNRENGGRETMYWLFHHIPEHLKRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P750</accession>
    <name>A0A1E3P750_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sorting nexin MVP1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Sorting nexin mvp1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_52136</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for vacuolar protein sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040385.1">
        <property type="nucleotide sequence ID" value="XM_019182685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P750"/>
    <dbReference type="STRING" id="683960.A0A1E3P750"/>
    <dbReference type="GeneID" id="30199931"/>
    <dbReference type="OrthoDB" id="10064318at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07597">
        <property type="entry name" value="BAR_SNX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06866">
        <property type="entry name" value="PX_SNX8_Mvp1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000042">
        <property type="entry name" value="Sorting nexin mvp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028662">
        <property type="entry name" value="SNX8/Mvp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035704">
        <property type="entry name" value="SNX8/Mvp1_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045734">
        <property type="entry name" value="Snx8_BAR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47554">
        <property type="entry name" value="SORTING NEXIN MVP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47554:SF1">
        <property type="entry name" value="SORTING NEXIN MVP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19566">
        <property type="entry name" value="Snx8_BAR_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="13">
        <location>
            <begin position="147"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="421"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ61178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="529" mass="61257" checksum="2827CADBC1E5A9E6" modified="2017-01-18" version="1">MSSDLFGHDDPADPWSLPGPKQWSTSSNTGIPQDLDQDYHNDENNNESEDDDDDVQIGNNSSGNNSHDNVFEETRVTSSINPLDSNGNNENNLHKSIWEENPILSEPLSASTLTGAAPHNELRNGDSNTGDFFSWSNAIRNTFNPLTRDIITISEIPEKEGLLFKHTNYLVAHLVPLPDTDATNDRKVVRRYSDFVWLAEVLLKKYPFRLIPELPPKKITSNSDVQFLERRRRGLIRFINQIMKHPILRTESLVLMFLTVPTDLSSWRKHANYDTTEEYKDVKISQRFLNLWDASIKQLWMDAQVIIKDAFESWTKLTVLVERFEKRSQQIAQDNERFQKILEDFKKTTPVLYSVENSDISSINEHLRIAGKHLSDSKQLLDDESQNIENGIVQDFKTYGDYLNSISGLFERYDRLGGNQIEQLTKKVQFNQQKLNELNGKPDARGVEVDRYSEAIKRDKIEIENQTNRDWLIKETVLSEFVLFQETQYQITKIFQDWVADKLKYSELHSNNWNQLANSLIDMPISRVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7B2</accession>
    <name>A0A1E3P7B2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_77818</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040377.1">
        <property type="nucleotide sequence ID" value="XM_019184617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7B2"/>
    <dbReference type="STRING" id="683960.A0A1E3P7B2"/>
    <dbReference type="GeneID" id="30201863"/>
    <dbReference type="OrthoDB" id="19690at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46115">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="119" mass="13826" checksum="CB1651189DD967B4" modified="2017-01-18" version="1">MTVQNQSELVQEIYNIDEFKSIIQDNELVVLDFYSTQCPPCVVVAPLYTHLAEKYSKAKFFKINGLLPEGAIVQKSIDVVWWPTFVIYKNGKEVWRVKIPNPPQQHPTAELEEELKKVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7L7</accession>
    <name>A0A1E3P7L7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_61431</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040074.1">
        <property type="nucleotide sequence ID" value="XM_019183173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7L7"/>
    <dbReference type="GeneID" id="30200419"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="615" mass="70928" checksum="DF63D5DFD145B6F4" modified="2017-01-18" version="1">MAKKRRISSVTVSSGNDPALDAKLLNSLIDQGVNFDLLANPDFKSFIEGNNPNFKCPSTNVANKFTEISASKYIQHVASTISNSDFPFVYLMMSFWRYSGIKLMNLNIQYYDNARRTCCIGVFKVKSFNEEAIANLIVNRLKELHLNKTVIGVTSDNSSFAKEIKKKLLQSSKLPMLKKECLFGCLINVLKDALYTFTGVYHQEMKLKKGTDHDSSAEASNTDSTEIEEQVFIKPELEDNYADDEEESEDEEEDEDELYRDKANKKQQQQQESEREEKLVHMNSIDSDYTVRLDNKPMSLREENHELKELNEILYYTVTTKVQRQRNFYSIGSCLKQLYDFLQDSELLLMDHLIDSSVHFGILENLIIRLSQIENLSKNLDHTPTLKAATITVKKLTDYWEEVKTNPILMMASQMFDLNKMKSLYSALNKDQENLLSEYLPKMGEHEQNNSIPTKKIESANIDSTNGFYHTNGNKRKRQLEDDRMLELREKLGLGVHRPGQESKENQDGSAELTHFKSFFMTSKIFKELKPNQVVKFYFQDNDDEDIEYNLKGLYPNISKLAKIAAITPVTLLECDRSSEYAKTVMFVNKFSTHINHVEKVSNLTIVKSFLKNHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7P5</accession>
    <name>A0A1E3P7P5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pre-rRNA-processing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_52587</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the RIX1 complex, composed of IPI1, RIX1/IPI2 and IPI3 in a 1:2:2 stoichiometry. The complex interacts (via RIX1) with MDN1 (via its hexameric AAA ATPase ring) and the pre-60S ribosome particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the IPI1/TEX10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040643.1">
        <property type="nucleotide sequence ID" value="XM_019182692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7P5"/>
    <dbReference type="STRING" id="683960.A0A1E3P7P5"/>
    <dbReference type="GeneID" id="30199938"/>
    <dbReference type="OrthoDB" id="361362at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120330">
        <property type="term" value="C:rixosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030174">
        <property type="term" value="P:regulation of DNA-templated DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024679">
        <property type="entry name" value="Ipi1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16056">
        <property type="entry name" value="REGULATOR OF MICROTUBULE DYNAMICS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16056:SF2">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12333">
        <property type="entry name" value="Ipi1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Pre-rRNA-processing protein Ipi1 N-terminal" evidence="8">
        <location>
            <begin position="127"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="343" mass="38368" checksum="7E88D0B4AAB44F68" modified="2017-01-18" version="1">MASTKKQKDKKKDFQKAKLKVGKTKAKPSNYTDTSFVAKTISLPNQSLSRNLDQDTDLIKRLSLVKHHASTTRKETLIHIEQHLPDNPSLYKQILNAVIPLIIDQSASVRTALISLLNAASEKQTNLMELHARSIILYIHSAMTHIVPEIRNDSTKFLDVLIKHGPDSLVRSAWIKTLKSFFTLLNWTLSDSKQSVSLAITTSSVTNNSGKSKKQGLDSLVRFIRIGAFANEDELSKKRIVLTAHPLTTKYLTPITPQPFAHLKLFTTELSSTNKNTVDKTIDLNTISCEDVETRKRVLVEFFIPQIKKNLTNLIKEGGEVGKSAKTLEAICNEVLSSKIIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P806</accession>
    <name>A0A1E3P806_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fumarylacetoacetase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_61570</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040214.1">
        <property type="nucleotide sequence ID" value="XM_019183201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P806"/>
    <dbReference type="STRING" id="683960.A0A1E3P806"/>
    <dbReference type="GeneID" id="30200447"/>
    <dbReference type="OrthoDB" id="3978420at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050163">
        <property type="term" value="F:oxaloacetate tautomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.850.10:FF:000002">
        <property type="entry name" value="2-hydroxyhepta-2,4-diene-1,7-dioate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820">
        <property type="entry name" value="ACYLPYRUVASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820:SF112">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_1G02370)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="3">
        <location>
            <begin position="71"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="283" mass="31405" checksum="6849AABD9FB8680F" modified="2017-01-18" version="1">MTFKRLVRFIASDGKTYFGDAILQTKQSDPRLATQAYKISGNIFGSYKVTDEVLSIDKLLPPLDHSLVHTIRMIGMNYKKHAIEINLPIPKYPCLFYKPVSTLNGPSDDIIIPKVAQIEDIRIDYEAELVVVIGKQGSDIEESKALDYVLGYTAGNDVSQRTWQIERGGSQFSTGKMFDTWAPIGPAIISPELIQDPNGLKISSKINGEIRQNSVTNDMIFNVRKLISFLSQGTTLLPGDILFTGTPQGVGSGFKPPKWLKNGDIVEINVEKIGTLQNKINFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P841</accession>
    <name>A0A1E3P841_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TECPR1-like DysF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_25178</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040780.1">
        <property type="nucleotide sequence ID" value="XM_019181112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P841"/>
    <dbReference type="STRING" id="683960.A0A1E3P841"/>
    <dbReference type="GeneID" id="30198358"/>
    <dbReference type="OrthoDB" id="74314at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032581">
        <property type="term" value="P:ER-dependent peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052816">
        <property type="entry name" value="Peroxisomal_Membrane_PEX28-32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010482">
        <property type="entry name" value="TECPR1-like_DysF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28304">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28304:SF2">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06398">
        <property type="entry name" value="Pex24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="TECPR1-like DysF" evidence="8">
        <location>
            <begin position="40"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="404"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="451" mass="53129" checksum="E2CE79200E8A584E" modified="2017-01-18" version="1">MSQQLTDKLIEKLISIALPPTNEFSLDSLEGRVQAGKLRPGLSVQTMSRNFIQLNSRLSFPFEMIDNIIKFFNWDNPKLTLTIMVLYTHLILKPLPMMLSLPFCLIIIYIMAPAYAIRHQPEYHSIIGKNNPILAEGPQLKDYEIPKPAPEISREFILNLTDLQNHMLLYVIAWDFINNLFAKFAYFIDESISSFFYLCFLMIGGFTILFTESIISFIPIKFLLIVAGWGFTIIFHPYFKDYFFNLVYSEETRYYLLNKTNKFEQILEENFGFIEPQEQREVEIFEIQNFDKKLKEWNFLFYLNNDFTKLSKPRLDKKYTNVEIIGTTSLQNVKPPKDWEFIKNDQWKIDLNPESWVSNHLLNDLVKVDNETKWVYDVDNDIKGEFRRRRWTRSVTRFSEIEFQKHNNNNTENHNNNDDEKPGLSPSVSHFETDHTSPLVAPVSPNSGEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8Q4</accession>
    <name>A0A1E3P8Q4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein bS18m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_16455</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS18 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040897.1">
        <property type="nucleotide sequence ID" value="XM_019180691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8Q4"/>
    <dbReference type="STRING" id="683960.A0A1E3P8Q4"/>
    <dbReference type="GeneID" id="30197937"/>
    <dbReference type="OrthoDB" id="21463at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.10">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001648">
        <property type="entry name" value="Ribosomal_bS18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036870">
        <property type="entry name" value="Ribosomal_bS18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479:SF40">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS18M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01084">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00974">
        <property type="entry name" value="RIBOSOMALS18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46911">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="105" mass="12109" checksum="3F7A916B202295ED" modified="2017-01-18" version="1" fragment="single">KTSENTNIARQFQTNFPIGTTYDPFDFSMAKIHLEKKKLREKRLQTNGFDRKNLNPLDFYTTPRYLSAYLSNTGKILNREVTGLSNKNQKRLTVAIKRAINAGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8W5</accession>
    <name>A0A1E3P8W5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_59925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041056.1">
        <property type="nucleotide sequence ID" value="XM_019182904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8W5"/>
    <dbReference type="STRING" id="683960.A0A1E3P8W5"/>
    <dbReference type="GeneID" id="30200150"/>
    <dbReference type="OrthoDB" id="2135762at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000444">
        <property type="term" value="C:MIS12/MIND type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013950">
        <property type="entry name" value="Mis14/Nsl1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31749">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN NSL1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31749:SF3">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN NSL1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08641">
        <property type="entry name" value="Mis14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="127"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="215" mass="25134" checksum="C721ACB71F2A1B3A" modified="2017-01-18" version="1">MSQEHHQKIHLNLKDVKHLQIQFAKAATDKLNLYLPTSDHSDPLKSRVSSLVNDFIYEIFEASKNSLDIDGATGNRDSLRSLLENSTNEVEPFDFSLQDQVRKAYQEVEQETIKLTKMRRDAPSEIKKSYEDSLEQSLSKIETLQKELENLESENEADIENHEDQFKRGVSSRLEDMISDYEESMRSIKEIKDQMPQERNEIEKLNNIIEFLQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8Y0</accession>
    <name>A0A1E3P8Y0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_81911</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040881.1">
        <property type="nucleotide sequence ID" value="XM_019185310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8Y0"/>
    <dbReference type="STRING" id="683960.A0A1E3P8Y0"/>
    <dbReference type="GeneID" id="30202556"/>
    <dbReference type="OrthoDB" id="4064185at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="53843" checksum="61D906D6D174E839" modified="2017-01-18" version="1">MKNPLEEEIQLAKVLNLLKLYELKLTEFQANLIFEKVPEEASGFVEKFISKTVRSHLKNEDTDVLLSIGYRSIMKDSSLNNPAGCHETWLNIKHLRNNIAQHEHKILGVLLRVFNKNKKFRRLAMDIANELPRSSYSDPALTGPLIHLSISMKRPQLANAVCSTIKPPISRELLSDLLVMHLSFGDSEGTERVLHQIKEMDSELSARDYMVIISQLLRRNKFDEALKILKTIPLGCAMISYSTMINHFVKQSISQGDTISPDNLAIIDELITKASTVSVDGFWDRLAPMYIKYLVSSGLKADLETAQRIYINTFLDFKAKEYLENHLSLKLNYSLDDTILTTNPFLIEDEHQKGLIYLRATDGCKVIILKIIADAAIRIGNVEGLTFALNQLGEAGYTKNEIILDYSRKYRKKASQKGFNPRRLRSYELNDDHRFEDIRENDGLEKLKFLKKFNRKSASKVKQMSDTRFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9B8</accession>
    <name>A0A1E3P9B8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_26541</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041212.1">
        <property type="nucleotide sequence ID" value="XM_019181275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9B8"/>
    <dbReference type="STRING" id="683960.A0A1E3P9B8"/>
    <dbReference type="GeneID" id="30198521"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035841">
        <property type="term" value="C:new growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035840">
        <property type="term" value="C:old growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030950">
        <property type="term" value="P:establishment or maintenance of actin cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902660">
        <property type="term" value="P:negative regulation of glucose mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903139">
        <property type="term" value="P:positive regulation of cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070610">
        <property type="term" value="P:regulation of fungal-type cell wall (1-&gt;3)-alpha-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000573">
        <property type="entry name" value="RHO family GTPase Rho2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ62005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="189" mass="20919" checksum="BF852EC7714AAC53" modified="2017-01-18" version="1">MSSSVKRKLVIVGDGACGKTSLLYVFTLGEFPTEYHPTVFENYVTDCRVDGKAVQLALWDTAGQEEYERLRPLSYSKANVILIGFAIDQPDSLQNAKAKWSDEVSRYCPFVPVILVGLKKDLRNNSPDKTNRKKFVQPALGEEVARLIGAKKYLECSALTGEGVDDVFEAATRTSLLVLNERSSCCTIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9S8</accession>
    <name>A0A1E3P9S8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_25123</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. Bacterial non-heme haloperoxidase / perhydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040844.1">
        <property type="nucleotide sequence ID" value="XM_019181104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9S8"/>
    <dbReference type="STRING" id="683960.A0A1E3P9S8"/>
    <dbReference type="GeneID" id="30198350"/>
    <dbReference type="OrthoDB" id="408373at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000205">
        <property type="entry name" value="Non-haem bromoperoxidase BPO-A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050471">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43433">
        <property type="entry name" value="HYDROLASE, ALPHA/BETA FOLD FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43433:SF3">
        <property type="entry name" value="NON-HEME CHLOROPEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="22"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="273" mass="30229" checksum="155EF076F9DA658A" modified="2017-01-18" version="1">MPYLKLSDGTDLYYKDWGTGDPIVFSHGWPLNADAFEDQMIYLANKGFRVIAHDRRGHGRSSQPWDGNNLDQYADDLAELVHHLKLKNVVHVGHSTGGGEVARYIGRHGVNNVSKAVLIGAIPPLMLKTESNPNGTPIEVFDDIRSNVLKDRSKFFKELAPTFYGFNREGSAKSDGLIEGFWFQGMQASIKALHDCVTAFSSTDQTEDLKKMNVPTLVLYGDDDQIVPPIASSQAALKLLPQPTEKVYKGASHGVCSTHKDQVSEDILKFIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9U5</accession>
    <name>A0A1E3P9U5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_75863</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040864.1">
        <property type="nucleotide sequence ID" value="XM_019184276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9U5"/>
    <dbReference type="STRING" id="683960.A0A1E3P9U5"/>
    <dbReference type="GeneID" id="30201522"/>
    <dbReference type="OrthoDB" id="261831at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd15853">
        <property type="entry name" value="SNARE_Bet1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039899">
        <property type="entry name" value="BET1_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="39"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ61657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="129" mass="15130" checksum="A4A65139AE8B1503" modified="2017-01-18" version="1">MASSQLHQRERTSLFDQPQRVSSPYDKQTKADYNESELSHLESQNDDEMGVMSTKIQQLKGLSLRMGEEIRGSNKTLEELGNSFDQLGTRLKNTFNRMLVMAKRSGISIKTWFFLFVFVILFFFWVWIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9W6</accession>
    <name>A0A1E3P9W6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_87413</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041410.1">
        <property type="nucleotide sequence ID" value="XM_019186112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9W6"/>
    <dbReference type="STRING" id="683960.A0A1E3P9W6"/>
    <dbReference type="GeneID" id="30203358"/>
    <dbReference type="OrthoDB" id="1932312at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="275" mass="31018" checksum="C8927169CD718A22" modified="2017-01-18" version="1">MDGKIKLFKLDEDKVSMISEVQLHKRLVTDFKVWIDQDDGTPFICSIGWDFQLKISRIVNDSIEIISNFKLLSNATSIELGIYGKTPIVFLTRLDSTQLSLFTFKQPHIFEISRISLNDAEFSTHGFTPMSISLTSHKLDSNTFIAVGTSHVPYMRLIIIKLPPLDPLLTNLEIETEQHLTSAQVHRGLIIGNYNTLSPQDKYSQGVVHWRNDGSGVWISGDDGIIRGLDLKNGEVVQEHKGHDKRIKCLVVDELQGQGEVLISSGIDKKLIYWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA17</accession>
    <name>A0A1E3PA17_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_25521</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041463.1">
        <property type="nucleotide sequence ID" value="XM_019181155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA17"/>
    <dbReference type="STRING" id="683960.A0A1E3PA17"/>
    <dbReference type="GeneID" id="30198401"/>
    <dbReference type="OrthoDB" id="1658724at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000254">
        <property type="term" value="F:C-4 methylsterol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="5">
        <location>
            <begin position="146"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="307" mass="36397" checksum="6FA53B372CAC5418" modified="2017-01-18" version="1">MSQVFANATNILPTSQSYSDALSLISSTNPQLNIVEQYWAAWYHYMQNDVLATGLLFFLLHEFMYFGRCLPWYIIDKIPYFNKWKIQPTKIPSDKEQWECFKSVIMSHFLVEAIPIWTFHPMCQKLGISISVPFPTLKTMVLQISLFFFLEDMWHYWAHRLFHYGPFYKYIHKQHHRYAAPFGLTAEYAHPVEIMSLGAGTVCMPILYVYFTKNLHLFTLCVWITLRLFQAVDSHSGYEFPWSLHHFIPFWAGAEHHDLHHHYFIGNYASSFRWWDFSLDTEAGPEAKAEREERMRLKAEQKAKKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA32</accession>
    <name>A0A1E3PA32_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_82349</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041482.1">
        <property type="nucleotide sequence ID" value="XM_019185368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA32"/>
    <dbReference type="STRING" id="683960.A0A1E3PA32"/>
    <dbReference type="GeneID" id="30202614"/>
    <dbReference type="OrthoDB" id="3357846at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015244">
        <property type="term" value="F:fluconazole transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF23">
        <property type="entry name" value="FLUCONAZOLE RESISTANCE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="431"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="463"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="500"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="84"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="544" mass="60463" checksum="6A56F117BE9F6FD3" modified="2017-01-18" version="1">MSSAKILIRGSLLGRFIYRVSSRKYLGYKEESKDYVIPEKYLEPYQKPEIDSSDESTLTLDKSKFIIVDWDGDDDPDHPNNWPIWIRIFVGLFISTLSAFVYMASAVYLPAIEELQDEFHIGHVVATLPLSLFVIAYGLGPLIFSPLSEHPSIGRNSLYVITLVIFAILQIPTALAKDIASFCVLRFIGGIFASPALATGAATFADMYSLPYMPVGLALWGIGSFSGVSLGPLIGSALEVSTHGWRWSFWFMLILSSFLALAFATIIPETYHQTILAGKAERLRRITGNPHIVLESDLHTHDKSPSEVLTEILWRSVEIGLREPVVVMINLYIALLYAVMYLWFEGFPIYFAETKHFSTMATGVAYVSIIVGVALGAFLYIFLIFQDYTKPMLRKEKVHPEAFMRASIIGSIILPIGLFIFGWSATRDHHWIGAIIGAAIYGFAGIMMFQTLLNYIGMSFHRYVASAFASNAFLRSVVAGCFPLFGRAMFKNLSTSQFPVAWGSTILGIITIMMISVPVLFYLKGPKLRASSKYSGTGREGGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAG4</accession>
    <name>A0A1E3PAG4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_76393</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041421.1">
        <property type="nucleotide sequence ID" value="XM_019184373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAG4"/>
    <dbReference type="STRING" id="683960.A0A1E3PAG4"/>
    <dbReference type="GeneID" id="30201619"/>
    <dbReference type="OrthoDB" id="550575at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="2"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="713"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="833"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="718"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="736"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="747"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="833"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="900"/>
            <end position="912"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="912" mass="104567" checksum="D0DD4EF0327CDFE8" modified="2017-01-18" version="1">MGTNLDSLPREVIGQIVGFLKADEAAKLRQLSKAIKSGVDASPLWKKECHNLWLSKFDQRNDKVDDNTNYCQYFNTRMRIDHGVVKILDQIVESDSSDYVFNECWKIVQKKNLVTPVLNRIRKDPKNKFRLSVRYFATALLRSIRRATAYETIDRCVAKGTSPYTSVSTTYEETLYNLSHFDPGFEDAAPYRERTLETIRKEAQKDPKFKKAPIGEKVAIIYRSVTSVLHGLDSPLARGDTSEDNSIVRLYAKEVLGHPITAIAIVERIARDFNIPVQIMEHKYIESGKIPAYILRVGVSSDFDYVWITGPVMHNHTFRDITYNCVKAGSLLQTFRQLEHATESSVGRILKPMELANQIGMYTQNFQLFESKLKQDEENKPLSKLYPITSSEYSEMYHQALYDYYWFVQSSGKKFRKSVYFGVAQDGMFKKHTSQGFAAALSKQFPFDLPLIHNKKVFHDMPLGQYQRYVKKDYFNFQDSLDLRYNVPEDIKKSVSQRFKPGDTCYDEIYNDFKGDFGVILGYQYTFNPENPDLVYRLATSGRHYISKNRVIVDGQCLPRVFDVICENIGIGLFMKSFDPKKMKFEPTPFLKKSGSTTLRRFNPFTTDAVDDMLARQGFDVDSEYLDHDTGFFGEGVEIYDSNAQYTSHANPEDDEESEGEGIGPEMIRQFMTSNVNESPGNVAEFLRMMAAFDGLGAGEGYDLDGERYEHNYFHSDYDDDDDDDDDEDEDEDEHYYDRNLGHEHDSEDDYEDEDEDEDEDEDEDEDEHFDPVDPHGVIPPGISPNIAREGLTPLFSMLQAMGADTDNVVEMLNRQGTRIAEFHNQQQEIRNRNGENTGASTQRVFTPSNPASLSDLQNRNAEPVVQPRASQPRSAPQPRTTQPRAPEPRAPEPRAPAQESEDEHDELWDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAJ5</accession>
    <name>A0A1E3PAJ5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">4-hydroxyphenylpyruvate dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_27477</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="10">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 3/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the 4HPPD family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041527.1">
        <property type="nucleotide sequence ID" value="XM_019181377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAJ5"/>
    <dbReference type="STRING" id="683960.A0A1E3PAJ5"/>
    <dbReference type="GeneID" id="30198623"/>
    <dbReference type="OrthoDB" id="414569at2759"/>
    <dbReference type="UniPathway" id="UPA00139">
        <property type="reaction ID" value="UER00362"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003868">
        <property type="term" value="F:4-hydroxyphenylpyruvate dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07250">
        <property type="entry name" value="HPPD_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08342">
        <property type="entry name" value="HPPD_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.180.10:FF:000001">
        <property type="entry name" value="4-hydroxyphenylpyruvate dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005956">
        <property type="entry name" value="4OHPhenylPyrv_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041735">
        <property type="entry name" value="4OHPhenylPyrv_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041736">
        <property type="entry name" value="4OHPhenylPyrv_dOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01263">
        <property type="entry name" value="4HPPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959:SF1">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009283">
        <property type="entry name" value="HPP_dOase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="VOC" evidence="11">
        <location>
            <begin position="33"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="11">
        <location>
            <begin position="207"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="376"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR009283-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="418" mass="47185" checksum="51441F42652CE1BC" modified="2017-01-18" version="1">MQKTNQVSVEAEGSTVEQQEADIGADISKCFSGYHHVKWYVSNAKQAASYFIHCFGFQWIAYRGLETGSKAISGHVVKNGDVVFEFVSPIVVSSSDPIVNSQLNDIHNFIKIHGDGVKDVAFGVNNVPMVYANAIKNGAKEVRSPQKLGDHYGIVECATVEVFDDVHHTLIDSSGYNGLFLPAYVFVTDSKSSEFYLNQLPKVKFLQIDHCVQNEDWNQLERACLMYSKIFGFHRFWSVDEKAVSTQYSALRSVVMASSNELVKMPINEPAEGLFKSQIEEFIEFNNGSGVQHIALLTDDIITCVKNLRSRGVLFIDIPAKYYDNLKRRLQQFNIKLNENLSELQKLGILVDFDENGYLLQLFTKPLSDRPTFFMEIIQRNKHNGFGAGNFKALFESIESEQKLRGTLEKSTHSTIME</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAL0</accession>
    <name>A0A1E3PAL0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein LOT5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_82488</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LOT5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041663.1">
        <property type="nucleotide sequence ID" value="XM_019185387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAL0"/>
    <dbReference type="STRING" id="683960.A0A1E3PAL0"/>
    <dbReference type="GeneID" id="30202633"/>
    <dbReference type="OrthoDB" id="19714at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039924">
        <property type="entry name" value="ICln/Lot5/Saf5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03517">
        <property type="entry name" value="Voldacs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="211" mass="24659" checksum="0B736AC081E08B8A" modified="2017-01-18" version="1">MVVAANDSSDLLVDLFAFNTSFIIWFNGLEKGLEISYLDIPAHAVRKNQLGQLELYIQMNYSNSGDQSPCLIEFSFTPKYWENDRYFNQEIEKLFTYEFFGLNKGSKMIFNTFNAIGKCSCFHNNNSDDYDDERSMDDADEVIDEFYEMGLDNSGNADDMTDTNEGEIKWNSGVNVKIYNDEEKFKTRSLEELDVEHNYRRFKYFKNSFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAM9</accession>
    <name>A0A1E3PAM9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_86967</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041134.1">
        <property type="nucleotide sequence ID" value="XM_019186079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAM9"/>
    <dbReference type="GeneID" id="30203325"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133788" evidence="1">
        <location>
            <begin position="17"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="56" mass="6430" checksum="C244C1B488AE4534" modified="2017-01-18" version="1">MLQVLGFCVTWPLLQSLVLFDDFKIVYPNNIVWINYGSCDIDFNSNSLFDLIKAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAZ1</accession>
    <name>A0A1E3PAZ1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL15/eL18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_12757</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041745.1">
        <property type="nucleotide sequence ID" value="XM_019180544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAZ1"/>
    <dbReference type="STRING" id="683960.A0A1E3PAZ1"/>
    <dbReference type="GeneID" id="30197790"/>
    <dbReference type="OrthoDB" id="361383at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000017">
        <property type="entry name" value="Mrpl10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_uL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_uL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005749">
        <property type="entry name" value="Ribosomal_uL15_bac-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01071">
        <property type="entry name" value="rplO_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934:SF11">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="5">
        <location>
            <begin position="79"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="223" mass="24922" checksum="1DD4B08D128C8EA8" modified="2017-01-18" version="1" fragment="single">STRSVSLLSSLKPSYNSTSSIKRVGRGPSSGYGKTSGRGQKGQKARGKVKSWFEGGQTPIFKLFPKIGFNNVKALQLKEVNLIRIIQFHQDGRLDLKEGETLNMRKMKQIGLVTGTLRDGVKILATGKERLDFPIKIEATRASAEAIKAIERAGGKFTARFFTKLGLRAHLHPMHFLKTRGYIPLPGRPASKRNIDYYSNPRKRGYLIKENDPLLQAIRDAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB21</accession>
    <name>A0A1E3PB21_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pre-mRNA-splicing factor CWC2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_21320</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1, U4, U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the 'nineteen complex' NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM CWC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041775.1">
        <property type="nucleotide sequence ID" value="XM_019180901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB21"/>
    <dbReference type="STRING" id="683960.A0A1E3PB21"/>
    <dbReference type="GeneID" id="30198147"/>
    <dbReference type="OrthoDB" id="10251848at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045787">
        <property type="term" value="P:positive regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033120">
        <property type="term" value="P:positive regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032297">
        <property type="entry name" value="Torus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF2">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16131">
        <property type="entry name" value="Torus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="9 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="18">
        <location>
            <begin position="63"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="17">
        <location>
            <begin position="126"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="16">
        <location>
            <begin position="63"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ62568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="226" mass="25831" checksum="4D36632BBE0AC43D" modified="2017-01-18" version="1" fragment="single">KPARVQVDPETIPQDDAPPQTGTVFNIWYNKWSGGGNQFQLVKSKYKLNVEKDSGYTRANKIDGQKYFCLFFAKGMCTKGRKCEYLHRIPNDLDFFPQTVDCFGREKFSEYRDDMSGIGSFNTVNKTLYIGGLIIKDNTQDLLNKEFRKLGKIAKINVINNKNCGFITFKNESSAQFAKEAMFGQSLYGTDILNIKWANEDPNPAAIKAKKRQHEEETQQVVEQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB83</accession>
    <name>A0A1E3PB83_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SRR1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_25948</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SRR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041845.1">
        <property type="nucleotide sequence ID" value="XM_019181209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB83"/>
    <dbReference type="GeneID" id="30198455"/>
    <dbReference type="OrthoDB" id="551431at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012942">
        <property type="entry name" value="SRR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040044">
        <property type="entry name" value="SRR1L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28626">
        <property type="entry name" value="SRR1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28626:SF3">
        <property type="entry name" value="SRR1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07985">
        <property type="entry name" value="SRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRR1-like" evidence="2">
        <location>
            <begin position="23"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="226" mass="25993" checksum="8369009E983A65E1" modified="2017-01-18" version="1">MKASLEKKKHTIKSSTLYENISSTLQPHKLSKIRCVALGSPSQEEPALYQLALLLIIVEENSIESQNVSLYDPVFTPLDIDFFKSLNFEVSEDYLPKDDEATLFFLPHAPLNLTNSVILSQNPKLLLSNNIQTHTERLTKLELFEKYPIMSKLVNLLQPQGDKTDGFEAVVKKKSRKSQRNKFVEPTIDYSKIDSYFENIEIISFKDFEEGEWLNSFTDLAFHRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBB8</accession>
    <name>A0A1E3PBB8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_60725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041875.1">
        <property type="nucleotide sequence ID" value="XM_019183045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBB8"/>
    <dbReference type="STRING" id="683960.A0A1E3PBB8"/>
    <dbReference type="GeneID" id="30200291"/>
    <dbReference type="OrthoDB" id="2535391at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="247" mass="27431" checksum="906FB5C6BA3174A4" modified="2017-01-18" version="1">MKIPYYLHSAYLKSNNLSKIKQLEKDNYLLKSSTNSRSLERLPLRPETIDLTANDSIDGLPGMDQRKVVTRSFVNQIKQSSSQRVVPSGSSRNHAPTHSNQSFEITKGRMAESTNILNNSPIKSIVRNNLQSMRNGSHGLSTPTSHSSHRVMSNDSFNSTLSSKTPEPFFPPTANMAMNQKKQSGSNRQLESNRVTKNRASTSQIAQRLSAHMKIGSATPSTGLRVQTSNGIRPGSSFTQNNIFRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBH2</accession>
    <name>A0A1E3PBH2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Crh-like protein</fullName>
            <ecNumber evidence="14">3.2.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_87537</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the glycosyl hydrolase 16 family. CRH1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.2.-.-" evidence="14"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041444.1">
        <property type="nucleotide sequence ID" value="XM_019186115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBH2"/>
    <dbReference type="STRING" id="683960.A0A1E3PBH2"/>
    <dbReference type="GeneID" id="30203361"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06923">
        <property type="entry name" value="ChtBD1_GH16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02183">
        <property type="entry name" value="GH16_fungal_CRH1_transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000159">
        <property type="entry name" value="Glycosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017168">
        <property type="entry name" value="CHR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF22">
        <property type="entry name" value="GLYCOSIDASE CRH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037299">
        <property type="entry name" value="Glycosidase_CRH1_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7 14 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133797" description="Crh-like protein" evidence="17">
        <location>
            <begin position="20"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="18">
        <location>
            <begin position="60"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="333"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="347"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="389"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="15">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR037299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR037299-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ62237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="443" mass="48000" checksum="EC5B02513888C483" modified="2017-01-18" version="1">MHLSNRFILTLSVITYVSAAGILCNSSSSCPEDLPCCSQYGECGVGSYCLGGCHPVFSYSIDSCMPMPVCKNLTTSFSDIDDLINQEDYLGDASDGDWIYNGYIDKFDDSLLLAMPNQSSGTVLSSTHYVWYGKISAHLKTSHLQGVITAFILFSNAQDEIDYEFIGSELDLVESNYYYQAVLNYTNVKNTTVSNTYENFHTYEIDWTPEELTWSIDGNAFRTLKKADTYNESTESYHYPQTPSRVQISLWPGGDSKNKIGTVAWAGGAIDWNSDDIKDNGYYYMLLKNISVECYVPPSSIHIEGDQSYVFNNSKSFDQDDIIITDEDTVLGSKKATGLDPDEGKESSSASSSSSSSTDQSTKTKSSSGGKSTGSSENTTKKDGGDKGGSSSTSAATGFVQFGESTSASGGKDASSSNGGIMSLPENSIISIVVSFLMFFTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBH9</accession>
    <name>A0A1E3PBH9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fimbrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_76918</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019184468.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3PBH9"/>
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    <dbReference type="OrthoDB" id="431378at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099079">
        <property type="term" value="C:actin body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120106">
        <property type="term" value="C:mitotic actomyosin contractile ring, distal actin filament layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051639">
        <property type="term" value="P:actin filament network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044837">
        <property type="term" value="P:actomyosin contractile ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070649">
        <property type="term" value="P:formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd21294">
        <property type="entry name" value="CH_FIMB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21300">
        <property type="entry name" value="CH_FIMB_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21303">
        <property type="entry name" value="CH_FIMB_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000042">
        <property type="entry name" value="Fimbrin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000010">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000016">
        <property type="entry name" value="Probable fimbrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000027">
        <property type="entry name" value="Probable fimbrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039959">
        <property type="entry name" value="Fimbrin/Plastin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961:SF18">
        <property type="entry name" value="FI19014P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961">
        <property type="entry name" value="FIMBRIN/PLASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="124"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="272"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="396"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="519"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="628" mass="69802" checksum="CB75C253186A8BDD" modified="2017-01-18" version="1">MNIVKLQRKFPVLTQEDLFNVIEQFRALDVEDKGYAEKQAVIEKISDSGEGSYDDVRQTLKQVSVDSSGRVELDDYVELVAKLRENKTAPKSAQSSAIQHKGTGAKASIIVGGSTSGSTHTINEEEKSEFTKHINSVLAGDADVGDRIPIPADTFQIFDECRDGLILSKLINDSVPDTIDTRVLNFPKKGKALNNFTISENANIVINSAKAIGCVVVNVHAEDFIEGKEHLILGLIWQIIRKGLLNKIDIKLHPELYRLLEEDETLEQFLRLPPEQILLRWFNYHLKAANWNRRVTNFSKDVADGENYTVLLNQLDPQLCSLQPLQTQDLLTRAEQVLSNAKKLDARKYLTPSALVAGNPKLNLAFVAHLFNTHPGLDPVEEAEKPEIEEFDAEGEREARVFTLWLNSLDVQPPVVSLFEDLKDGTILLQAFEKVLPGSVSPKHINKRPSSGADISRFKALENTNYAVETGKANHFSLVGIEGSDIVDGNKLLTLGLVWQIMRRNIVLTLKSLSANGRELSDSDILKWAKEKVAKGGKSTTVRSFSDPSIANGVFLLDVLNGLKPGYVDYDLVTPGHSEEDRYANARLAISIARKLGALIWLVPEDINEVRSRLIYTFVGSLMAIEHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBQ8</accession>
    <name>A0A1E3PBQ8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetyl-CoA acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Ergosterol biosynthesis protein 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_27319</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.9" evidence="2"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042060.1">
        <property type="nucleotide sequence ID" value="XM_019181356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBQ8"/>
    <dbReference type="STRING" id="683960.A0A1E3PBQ8"/>
    <dbReference type="GeneID" id="30198602"/>
    <dbReference type="OrthoDB" id="5404651at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000007">
        <property type="entry name" value="acetyl-CoA acetyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919:SF165">
        <property type="entry name" value="ACETYL-COA ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="10">
        <location>
            <begin position="6"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="11">
        <location>
            <begin position="274"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="8">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="353"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00084041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="404" mass="42157" checksum="4FBD6BEE6CF8590A" modified="2017-01-18" version="1">MSENSVYIVATARTPIGSFQGALSSLNYVDLGAHAVTAALSKVPEIKPEQVEEIVFGNVVQANVGQNPARQVALKSKLGVDTVATTVNKVCASGLKATIIAAQTILLGNADIVVAGGAESMTNAPYYVPAARSGARYGDSVLVDGLQRDGLADAYDGTAMGVSAEKCARDYNLSREDQDNFAIKSYQKSQKAHESGKFKNEITPVVIPGFRGKPSTTVDVDEESYKLNVDKLKSARTVFQKENGTVTAANASPINDGGAALVLVSHKKLVELNLKPIARILGWGEAAQNPEDFTTAPSLAVPKALKHAGLKIEDVDFFELNEAFSVVGLSNQKILGIPEEKLNVYGGAVAIGHPLGCSGARIVVTLASVLQQEGGKIGVAGICNGGGGASSIVIEKLSSEHISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBT4</accession>
    <name>A0A1E3PBT4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_99129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041967.1">
        <property type="nucleotide sequence ID" value="XM_019186681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBT4"/>
    <dbReference type="GeneID" id="30203927"/>
    <dbReference type="OrthoDB" id="3996692at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="143" mass="16390" checksum="0122C21B8EF546C8" modified="2017-01-18" version="1">MKEIYDLQKQVALLKIDNLGYIHTNGDLKSTTSSPDKPSLSPISPVTGLTVTTVENNNENSRSKYYSNPKTPLLQQNINTHRNGSPSSPKFNSSISVLRNEFKKLVNEMHTTYKSELEKEKVERKRLEHLLKLYEERSNDIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC10</accession>
    <name>A0A1E3PC10_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_82524</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041709.1">
        <property type="nucleotide sequence ID" value="XM_019185391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC10"/>
    <dbReference type="STRING" id="683960.A0A1E3PC10"/>
    <dbReference type="GeneID" id="30202637"/>
    <dbReference type="OrthoDB" id="3064516at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05674">
        <property type="entry name" value="M20_yscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017141">
        <property type="entry name" value="Pept_M20_carboxypep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047177">
        <property type="entry name" value="Pept_M20A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE/HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962:SF1">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE_HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037217">
        <property type="entry name" value="Carboxypeptidase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="8">
        <location>
            <begin position="307"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="509"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="539" mass="60599" checksum="5900AE90689C00BD" modified="2017-01-18" version="1">MILSLISYSIYTHISCFNETAIEIPPNPYANIKPLRPSDSKKAHFIYTDPEFRNKSLETYQGILQIPTEVYDFTQRPEIDISGWEPFLKLHEYLEETFPLVFKHLKVEKINKLGLIVTYQGSDSSLPPLLLTAHQDVVPVDTDTLKSWKYPPYSAHFDGQFVWARGSADTKNLLSGIFETFEELLKDGVKPKRTIIFAGGYDEEAIGRYDGAVSLSSFLEERYGHKSFYGLVDEGGSVSKFGDSWIATPSIGEKGYLDIYIELFVPGGHSSIPPDHTSIGIVGELTTLIESNPYPSVLTLDNPILDLLRTVGETSNELDDSIRYDYIHAKFDEGALKRTLERLTKEKGTKYLVRTSQAIDIIQAGVKINALPEVLTLGINHRISLELTADEIIHNVVGHVEKIAKKHNLGLTWNNSEVILDTTDEGHFNVYSVQALDPSPLSTTNDKTWDVFASSIKHTYDDIVFKSSNDTISVAPSLVGGYTDASRYWPLTDHVYRFIAATGAGYDIHAINERFEFDSHLQTIAFLYDYILNVNEYDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC14</accession>
    <name>A0A1E3PC14_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mitochondrial resolvase Ydc2 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_67124</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042142.1">
        <property type="nucleotide sequence ID" value="XM_019184069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC14"/>
    <dbReference type="STRING" id="683960.A0A1E3PC14"/>
    <dbReference type="GeneID" id="30201315"/>
    <dbReference type="OrthoDB" id="3980521at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000402">
        <property type="term" value="F:crossed form four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070336">
        <property type="term" value="F:flap-structured DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039197">
        <property type="entry name" value="Mrs1/Cce1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015242">
        <property type="entry name" value="Ydc2_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28072">
        <property type="entry name" value="CRUCIFORM CUTTING ENDONUCLEASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28072:SF1">
        <property type="entry name" value="CRUCIFORM CUTTING ENDONUCLEASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09159">
        <property type="entry name" value="Ydc2-catalyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mitochondrial resolvase Ydc2 catalytic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="489"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="564"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="577"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="647" mass="73986" checksum="F4E2383E7F05313B" modified="2017-01-18" version="1">MAIDMGIKNFSFAKLQLDKTLPFNTPPLIKEWTKLDVNQWADVSSDASYEPSKYAELSYRVVDELIFNKDQDVPDVVLIERQRIRSAGGKNVLEWVYRVNMFETGNDAYENGDFKSHFEEDRLEHKKSKRDGEKIRSHRFTKVFRRKEKAQSPDYTSSPEDDCRIEKELSNSLDTYTSTKVTEKEVNSFQEESDDTSIQDSNVKIQTSKRHRSLRISWRSKRDVPSSSIKSSKSNKRSGFRSFFFRSKGPISVPSENKTESSVVTSSVYDVQETLTQRYMKDSSGLNQLLQDMDAIPFDLSVKKENTFHEKVTKVIQSLSPSKKSPEESTSRSDKDAEDDYSQDFETWNPYGRTNNKDKVDRISITSSEFELSKKYNVNLSVQRRPSKLGSFARSLSLRAKGKLRSHLNSDESLKEKEKEKFKENNEELSTNQKESLSSLFGQNLTKPSPNGGNNYQSDFSSASSTTVAGNDDLFGKLYAKTLDAVDKGSPSIENFSGSTKISTPSRNESSKIENIEDPKTPIEKSTPKPGSDAYHNVIVNFASIDDDTYDSEEVSSFLQDFYEPSRPTTKRDSRKTSCQLSRSNSRAFGSVRRNPLEFKKETQRPLTAVELFQKELDENKQELEELSFQIIRDRNHHRKVINGLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC54</accession>
    <name>A0A1E3PC54_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATPase expression protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_77153</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for translation of the mitochondrial OLI1 transcript encoding subunit 9 of mitochondrial ATP synthase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the AEP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042203.1">
        <property type="nucleotide sequence ID" value="XM_019184510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC54"/>
    <dbReference type="GeneID" id="30201756"/>
    <dbReference type="OrthoDB" id="10649877at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045182">
        <property type="term" value="F:translation regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031467">
        <property type="entry name" value="Aep1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17049">
        <property type="entry name" value="AEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 6" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="491" mass="55796" checksum="D6C3FEE58ABCCB05" modified="2017-01-18" version="1">MTSVLKKTVHKGSRSIDFINHNLKTSITQPKRGEIEKSLDPVNNISPVKNIKVYDHPIHPFHRPSAASKLIKVLRPHNKLDAGPIFSLESHTKGGHKVNLSPSFNSSREAIAFLDKLPQDYQILNPIEQPPISSSLSESAKSQIEEMRTDDVQSWSKILKARIVSKDGSIITNEDKLVFLLEKTINSTPESVSNVIPLLKSYITFVENSKLVSNISQFFLHQVLKYEYENPKQILQEISEIEKLLADEYPGVKKEPSFINVLLKTHLHLKEYAGTKLLIDSLIAQGFTPDAKTVEMYLKFHVDKLISSKTLSVTKNAQVKADFFDSIIDMDDVFHSVMNPNIAKLLLHGSDSLEEASSILKELQSKIQGQELKDTYKSFEVRMVNIMNQEDRTFVPHILQSALRNTFYQKHERVTEFANRSNVILLNRTVRYGSLIFATKLLEKVRVSPDDINGLSKILEQREFETINDSPGYDTRSRDAFLERLNAKSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCB6</accession>
    <name>A0A1E3PCB6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FK506-binding protein</fullName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_103379</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5 6">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FKBP-type PPIase family. FKBP3/4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041819.1">
        <property type="nucleotide sequence ID" value="XM_019180381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCB6"/>
    <dbReference type="STRING" id="683960.A0A1E3PCB6"/>
    <dbReference type="GeneID" id="30197627"/>
    <dbReference type="OrthoDB" id="77911at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023566">
        <property type="entry name" value="PPIase_Fpr3/Fpr4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43811:SF19">
        <property type="entry name" value="39 KDA FK506-BINDING NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43811">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001473">
        <property type="entry name" value="FK506-bp_FPR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="8">
        <location>
            <begin position="319"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="57"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="164"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="66"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="173"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="255"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR001473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="405" mass="44919" checksum="37FFDF51A27F4D95" modified="2017-01-18" version="1">MTSLLPLASYNLALNPFTPTQAIESELPVTVHLTLAAIDPEGAIDDDEQPSTLRILKSSAFRNLDEDSSDDDLSEDGEDAELNEDSDEDKIEEDSDEEDAGENDAEDDDEEDDEDFNDDEDDEIEEYVVCTLSPKSQYQQTLDLTISPDEEVFFVVTGSYPIHLSGNFVEHPYDEEDSEFDSDEDSEDEYDLSPDEDEIIGEDFDINDLEDESDVEGKIEELVEEEEAKKVEAPKETKKSKKENKKRQVEEEEAKPEPKETKKQKKAKEEKNVKFNKELEQGPTESKKESAPKSTTKTLPGGVVIEDRTVGKGPTAKNGSRVGVRYIGKLKNGSVFDKNTSGKPFNFGLGKGEVIKGWDVGVVGMAVGGERRIVIPAKMAYGSQKLPGIPANSELTFDVKLVSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCI3</accession>
    <name>A0A1E3PCI3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cell wall mannoprotein PIR1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_26623</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PIR protein family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042131.1">
        <property type="nucleotide sequence ID" value="XM_019181281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCI3"/>
    <dbReference type="STRING" id="683960.A0A1E3PCI3"/>
    <dbReference type="GeneID" id="30198527"/>
    <dbReference type="OrthoDB" id="5415592at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054508">
        <property type="entry name" value="PIR1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051153">
        <property type="entry name" value="Yeast_CWMannoprotein_PIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000420">
        <property type="entry name" value="Yeast_PIR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254:SF1">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00399">
        <property type="entry name" value="PIR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22799">
        <property type="entry name" value="PIR1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00929">
        <property type="entry name" value="PIR_REPEAT_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50256">
        <property type="entry name" value="PIR_REPEAT_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133815" description="Cell wall mannoprotein PIR1-like C-terminal domain-containing protein" evidence="9">
        <location>
            <begin position="19"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall mannoprotein PIR1-like C-terminal" evidence="10">
        <location>
            <begin position="226"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="59"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="309" mass="31030" checksum="A6C4C881D65D0A84" modified="2017-01-18" version="1">MQYRSALFTTALVSSALAAYVPSEPWTTLTPDASHPSGAVTDYASTFGIAVVPVTASSSGASASASPKAKRDAVSQIGDGQIQASTATPSSTKKAASTAQAVSQIGDGQIQASTSTAKPSSTAQAVSQIGDGQIQASTSTAKPSSTGQAISQISDGQVQASTSTKASVTASAASQIGDGQVQASSASASSALASASASQKADDSNPVSAVSCKSDGSLALTLKDSVLTDSAGRIGSIVANRQFQFDGPPPQAGAIYAAGWSITEDGYLALGDSDKFYQCLSGNFYNLYDENVADQCHEIKLEAVDLINC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCM6</accession>
    <name>A0A1E3PCM6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">V-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_21278</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041929.1">
        <property type="nucleotide sequence ID" value="XM_019180900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCM6"/>
    <dbReference type="STRING" id="683960.A0A1E3PCM6"/>
    <dbReference type="GeneID" id="30198146"/>
    <dbReference type="OrthoDB" id="190375at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15843">
        <property type="entry name" value="R-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051097">
        <property type="entry name" value="Synaptobrevin-like_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136">
        <property type="entry name" value="SNARE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136:SF168">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="76"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="153" mass="18368" checksum="FF29D79D29BCC089" modified="2017-01-18" version="1" fragment="single">LHLSLYFLKYILPSNDLVTVLTICSADIPRSFPLLTLDTIIKDYREYRETSNSNVEFKIKFNQIIKFQENQFQRNLIEITKNDLNQVKYILNDNIEQVLERNERINLLVNKTDRINTLSSNFRSNTTRVKRKMWWQNVKFWVLVVIFVVILLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUA1</accession>
    <name>A0A1E3NUA1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein L17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_81792</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL17 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454222">
        <property type="protein sequence ID" value="ODQ56664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035871.1">
        <property type="nucleotide sequence ID" value="XM_019185296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUA1"/>
    <dbReference type="STRING" id="683960.A0A1E3NUA1"/>
    <dbReference type="GeneID" id="30202542"/>
    <dbReference type="OrthoDB" id="275000at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.10">
        <property type="entry name" value="Ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000456">
        <property type="entry name" value="Ribosomal_bL17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036373">
        <property type="entry name" value="Ribosomal_bL17_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00059">
        <property type="entry name" value="L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL17M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413">
        <property type="entry name" value="RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01196">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64263">
        <property type="entry name" value="Prokaryotic ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ56664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="24584" checksum="976F730A17689641" modified="2017-01-18" version="1">MPVRHFSRTGSHRRAMMRNLVTSLIEHESIITTHAKAKETQKLADKLISLTKKDNITQAKVDAGEDLFKLKTTIPKLFQDLRTRFAKREGGFTRVLRLEPRLGDRAEQSIVELVDGPKEMKLWMTARVVARLQLQNLPVDDLTQHNVDKLIQFRAGGQEEFDALVERCKKEFYRDGKSEDLKNLPPPAENMRKPYTGYKLVKGFKVMPRPTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUM6</accession>
    <name>A0A1E3NUM6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nucleoside transporter FUN26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_13022</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036043.1">
        <property type="nucleotide sequence ID" value="XM_019180557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUM6"/>
    <dbReference type="STRING" id="683960.A0A1E3NUM6"/>
    <dbReference type="GeneID" id="30197803"/>
    <dbReference type="OrthoDB" id="46396at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034257">
        <property type="term" value="F:nicotinamide riboside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002259">
        <property type="entry name" value="Eqnu_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332:SF88">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01733">
        <property type="entry name" value="Nucleoside_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016379">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01130">
        <property type="entry name" value="DERENTRNSPRT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="344"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="380"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ56836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="406" mass="45258" checksum="B9E3C135B78F0D9C" modified="2017-01-18" version="1" fragment="single">VIFSFYNDNIQLSLSQLRYFTFLSIGISILWPWNCFLSASVYFIKKFGTGSGLSNNYSSTMMTVSTLTSFLLNSYLSTKQFANFVNRIKTGSLVNFSIFLLLSLIELFLPGLHNAGYFVFIMLLILFSSMGTCYQQNGCMAIVNVMGPIYAQAVMVGQAIAGVLPSIALMLSNLLYPGDANESSTSIVIYFFTTSIITLFSFVLLMITNRYKDDLPQDIEHESPVSETEYVPFKVLFAKLKFIVLSIFTVFVITLVFPVFASNITSVNPGFGKITTDAIYIPFIFLVWNLGDLAGRLACGFPQFVISSDRVLFLYSILRFAIVPFFFLCNLSKKDGDPAINSDFFYILLQFIFGFTNGHCLSCCFMNVGNYVEDEQKEAAGGFTTIFLSLGLAAGSIFSYLFVVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUS9</accession>
    <name>A0A1E3NUS9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nucleoporin NUP188</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_65670</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Nup188 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035997.1">
        <property type="nucleotide sequence ID" value="XM_019183962.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3NUS9"/>
    <dbReference type="GeneID" id="30201208"/>
    <dbReference type="OrthoDB" id="102511at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018864">
        <property type="entry name" value="Nucleoporin_Nup188_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044840">
        <property type="entry name" value="Nup188"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041634">
        <property type="entry name" value="Nup188_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048883">
        <property type="entry name" value="Nup188_N-subdom_III"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431">
        <property type="entry name" value="NUCLEOPORIN NUP188 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18378">
        <property type="entry name" value="Nup188_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10487">
        <property type="entry name" value="Nup188_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21093">
        <property type="entry name" value="Nup188_N-subdom_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup188 N-terminal" evidence="10">
        <location>
            <begin position="44"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin Nup188 N-terminal subdomain III" evidence="12">
        <location>
            <begin position="521"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="domain" description="Nuclear pore protein Nup188 C-terminal" evidence="11">
        <location>
            <begin position="1261"/>
            <end position="1552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ56790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1574" mass="177619" checksum="345091A3FEB24665" modified="2017-01-18" version="1">MTVENFGASLGGEVATSGASDNWSFELALSLLQIPHDDKRLKSLNEFLLKNQELLSTPLVFQKTSNDALPSKEKLKTYGEGVTEAHLKYAEEISKLLNISQKDAFRIIVDTLKRVPESNESQKTKTYSNDEFERQSKHKLYKLYISSIMRERRTVIRLIRTLVSGESIPFIEKKFSEKIFQDGEFISNAITSLQTFVDLINNNESDFADIISSEISLTIVEMLKLLISLLIVVSTTANNVVKWFNFLKSNKFFTDLKDLIPEEFYEEIEALRSITTLLFLGFNTNDDSFDPISPYLSDTNSIKSINSVLSKSSEDPLTLYAWTIILYTLDDAKTSVLFGETFAQVHGYFAVKAAELDVFKRIEHFHQALHYDNLYSAIISSFLIAAVPFIQLSDKISYTYLQIFKDTPNTFVEKFFTNEDTQRLLFLAKAKFPEVIIPYLRILSINGAFAHDELSKLSSYMHTISSQNLDYDNDTESDTIILKNGLYVNPPYEQNKDVLLYLPESTRGKLVPTADPKTEAAIFQYNYNGWALIGRIMQNVSGFEEDEEVLVTILELLSNTLGSVDYETSVDILESLSSFVDNGDILEIILKLFEQSLHQRNIRVLTPVADFLISLVGNFPQIVWSHLVRSDLLEHGGRGGLIATILGSVETVSGDYKFTISLLKLLNELVLNCISAPEDWTTQKNEVIPKFTSHAVQVFESFIYWNYKNSYQKFQIGTLIIDTFSKILYSVYGIDPESEINKKVTKSLAASASKVIKSFTNSFPDVRTIKPIIAAVELLDQTPAVFDTSGRVGFWFDQWSRASLEFSKLVVSIRSVIGSPPSTLEISLFTKAPLLINAYTKHTVLRGDILQLLTQLVSAKWPTDSPSLLSHLGDHHTGVLLASVSADLQHKFDDFKVKKYLYDFFSAVVEGNQKGLSMIFLNGRDIRDTTKKQSSILDILKNNVGRLDYYPESLSIHLVEAIAHAFNSWSSKKEENDVEFIQTLVKKLSDFKTGSSDLKNDDDIIQEAYRYKLNSRIAEICALFIFTSKDEKASKPIVDLLSDGKVVELIKPLYQPFGYRASLHENLNNNFESKWPSYKLQQFIRSPLANSTRYGENAVYDLRLLDDILGNNPYWTGNNLTKGYRSEVISASLNIQYVSSQISAAKSWGALLTAYVKKFKISSTFVEIIKKLLQANIDEGVKVNLFEEIYRVRVELSFFFLYSITKDRELKENEIVEIMRLALQLATSADVDFLGSVLSSKADIYRPLLRIVSNLLSASKKNTKVIEILSTDLLEFFEFVIAKGTSVILGSIQSDISSPDIGGRVEDLYLIISLFKGLVATNPPTSFTTKMSTLLVDFGTLKAILNVYSSSHLLKVNNEPLFADLTLSFMLELVSVDSIAEQLISSGLFSTLIQSPISLIIQEGNISVQSSPRYHNIWSNGLLAIILTLLSKFGARLLPEICVFVNYFIKQISSTIQSWSQDSLAITIPALQETEQIIILQRALKVLYYEFGLNIQNMQQQTAEIIPELDSKKGRKALSDAFTHLLAHPKFLTSRIIPTTFEEQRMFEGEDKIRTPLVEQLASQIGELKQSLTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUV9</accession>
    <name>A0A1E3NUV9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rrp15p-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_85944</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRP15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036186.1">
        <property type="nucleotide sequence ID" value="XM_019185973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUV9"/>
    <dbReference type="STRING" id="683960.A0A1E3NUV9"/>
    <dbReference type="GeneID" id="30203219"/>
    <dbReference type="OrthoDB" id="20949at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012459">
        <property type="entry name" value="Rrp15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13245">
        <property type="entry name" value="RRP15-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13245:SF14">
        <property type="entry name" value="RRP15-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07890">
        <property type="entry name" value="Rrp15p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ56979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="23658" checksum="40B2A213B8758C1A" modified="2017-01-18" version="1">MSKKRVSVQLPAKSASVTGEKRKRAEKPENDELDLGEVSSDEEEDSDDDDDENLPKLKKRKKAKDDGSESFANAVNAILGSKLKAYDRKDPILARSKQTIKKAESDKLEAKARRELLAEKKKVYDQDRIKDLLPTDDSKAREVLEHERKLKKIAQRGVVRLFNVVMSTQTRTSTEVNKTKVLGQEQKDKLITEISKEKFFDLVKAAGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV16</accession>
    <name>A0A1E3NV16_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_85986</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036248.1">
        <property type="nucleotide sequence ID" value="XM_019185980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV16"/>
    <dbReference type="STRING" id="683960.A0A1E3NV16"/>
    <dbReference type="GeneID" id="30203226"/>
    <dbReference type="OrthoDB" id="302728at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="11">
        <location>
            <begin position="112"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ57041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="345" mass="39382" checksum="6D3A6F6A9BD3FD56" modified="2017-01-18" version="1">MWQIEKFCCALASLFPKVLSNGLYLWAIYAFCYELCLRSIGGLTGLILASIGFYLAVQGIYMYYKVVKLGAGSPLDFAELRIDSDYNDLESSRTEPPQFLVDNSLMIKSNGKFRFCDKCKVWKPDRSHHCSSCNRCCLKMDHHCPWFATCVGFKNQKFFVQFLVNTVIFGEYALLISGIELYQFFNNEEYNDEFLSLNLVLLAVLGLTVTVAVGLFTGITLYFLSSNLTTIEYYDYMRYRNNLEIANDSYYRYSKQPSAKDLGNAYNLGSIKANLNAVLGTTWLEWILPIENKGFSDPYENRGLYFPSNPKIRAALVSNSKLQQQLLHELQRSSVSNSADTDRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV54</accession>
    <name>A0A1E3NV54_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PTM1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_81202</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036171.1">
        <property type="nucleotide sequence ID" value="XM_019185193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV54"/>
    <dbReference type="GeneID" id="30202439"/>
    <dbReference type="OrthoDB" id="3982857at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053938">
        <property type="entry name" value="PTM1-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21902">
        <property type="entry name" value="PTM1-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133535" description="PTM1-like N-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="PTM1-like N-terminal" evidence="3">
        <location>
            <begin position="60"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ56964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="459" mass="53477" checksum="B0CA4D0C24AC0AD5" modified="2017-01-18" version="1">MLKSLVFLIALIQLCLCDSNYTISDSYELCFSIDRADENQPILLDVALLEYEPLPGLTPEESKTSVPIIIYNENEINHEKQFAGVDSSTLNLCNDDAIKRGYCQKKHKGKILFEYNEGAGFKGNTYNEILNELGTFKDVFQFNKTGFYCARALGTNAKNFKVQLFIKGDSSTDHIIKKNRFVNLFSVFILVGFGGLYLSKWLDYSKTFDTKIPYLVKVLLAYIATQLTISVLKSVSAYIYDHPFESSNGHKVLSAISSSISKFQNAAMLFYLYQYSGNFTTNKIEFEYKKVFGTFCFVDMSAIVFYKLVRDHYYERYHEPGAFSLIYGSYELILILLFLFQLVIKVRIFLRYYRTAKGFYGFQQNPDHPKLDQFKWLILTNLFWELFEHMFHSMFEEMMHKVFRFGSHNLTNFMFNVIDGDYSQAFLDTIVLGVEFYVLLNFWTPDYFLIVEDYNKDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVI1</accession>
    <name>A0A1E3NVI1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Heat shock protein SSC1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_35932</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036416.1">
        <property type="nucleotide sequence ID" value="XM_019182374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVI1"/>
    <dbReference type="STRING" id="683960.A0A1E3NVI1"/>
    <dbReference type="GeneID" id="30199620"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000014">
        <property type="entry name" value="Chaperone protein DnaK HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000003">
        <property type="entry name" value="Heat shock protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000004">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000003">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="636" mass="69591" checksum="32B49791662874D3" modified="2017-01-18" version="1">MLRGLNKNLVKNLTNQRRYASVRSSLPTVIGIDLGTTNSAVAVMEGKEARILENDEGQRTTPSIVAFDKHGDMLVGNAAKRQAVLNTENTFYATKRLIGRRFDDDETKFDLNHFPYKIVENDNGDAWVESFGKKYSPSQIGGFILKKMKETAESQLAQNVKNAVVTVPAYFNDSQRQATKDAGQIAGLNVLRVVNEPTAAALAYGLDKTNDGLVAVYDLGGGTFDISILDIEDGVFEVRATNGDTHLGGEDFDILLVQFILSDFKHKTGIDLSSEPDKVQRIREAAERAKIDLSHVKETTIDLPFIKDTHHISISLTEDELDQMSFHLISKTIDPVKKALKDADLSKKEIDEIILVGGMTRMPKIRKTVEEIFGKKPSTAVNPDEAVALGAAIQGGVLSGAIKDVLLLDVTPLTLGIETYGGVYSPLIPRNTTVPVKKTEIYSTGVDNQTGVEIRVFQGERGLVQDNKLIGNFKLSGIPPLPKGVPQVEVSFDIDADGIINVSAREKTSGKETSITVIGNSGLTEEEIQKMIQEGEQNKDTDKAKRQKVEAATNAELVVNDTEMAMGLAFGDVLKADPDYPELENKIKDAKNAIQSARNGGDVEVADIKNISKELQELSIKIFQRVARKQNEAKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVY3</accession>
    <name>A0A1E3NVY3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_65587</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036538.1">
        <property type="nucleotide sequence ID" value="XM_019183944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVY3"/>
    <dbReference type="GeneID" id="30201190"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="149"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="242" mass="27548" checksum="93CE51D46BE70F24" modified="2017-01-18" version="1">MTRAFSLSTFDYNGRENSSKALIKGSSEDNDPLKKSASRVLKALLFPFPSNASTYKQVLNIMKMGSAETEAKAEEKKEKSEPIQQHGKTLQTKNIQKITRNDLINGTYDLNPKPIRTRSTADRARRHHRHHRHHHHNENGEIVHRSRSKTSTPNSIKSPSSSISPEIRNIAHIDDDAEKNEMFMAAHEQVIKDQMKKSKRASDSELLYQHTHTDDGTLTVPVHRTVTLDSDVSDYYSSYETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW19</accession>
    <name>A0A1E3NW19_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_65571</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036521.1">
        <property type="nucleotide sequence ID" value="XM_019183938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW19"/>
    <dbReference type="STRING" id="683960.A0A1E3NW19"/>
    <dbReference type="GeneID" id="30201184"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PPR-containing_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939">
        <property type="entry name" value="MEMBRANE-ASSOCIATED SALT-INDUCIBLE PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939:SF1">
        <property type="entry name" value="OS04G0684500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="72"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="209"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="625" mass="72878" checksum="317063EC6645ADD3" modified="2017-01-18" version="1">MELEQRDVANFYRYVSNHGFSLVYKEYLRISEVYGGDPPRQILRDAIGKAIRSSNMSALKKLCDVYKGKYNDMFTYHSFMAYYLQVGKPSKFFHVFREMANAGYEPDIRTYNLIFQQFSAKKDLESTFALLDYLTGKGMKVTIEELCHVLVCCAKQIDVDSATKVDQLADKYELKKTGEFYSSLMAVYTEANLHEKAIELFNSYDGTPNADLYANLIKTHVRKGDYDSAREAYKSMMEQNIEMTRKFYAVLIEYFCKIGEFTNARTLMDSIDLEDGNDMEPFAVMMENYMHKGLHAAAISVYNFLLENNVTPGPRVYNKLMECLVQLSFVNNHNFSRPARIAEELLATQKENPKKHYLSFKIIKPLVRYLSKQYDPVYALKLLEDYKSSRSEYSTSKNISILKEELIIYGETKQYYRFNAVFENFLDEIKRRMVRGKRPHKSKDVFLPIIGYRARSGAVLDEQKQLQDLVQNFIFAHNFNVSNENLNEIADILLADSRTFMFGMEIVETKLMGGFIAHAVVRAREWRNSVLPLGANPIEYQNPITMPKIYLKDEFYLKIAIHLDNYLSMIRKMDPNKMPDFMEKFRIKFPKVFKNFPGTISNFADVEGYDYKKDFRDSGIANDRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW43</accession>
    <name>A0A1E3NW43_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_64777</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW43"/>
    <dbReference type="GeneID" id="30201046"/>
    <dbReference type="OrthoDB" id="10547871at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="516" mass="60314" checksum="D88C21AE4015D144" modified="2017-01-18" version="1">MIVPTGPSKSIKINIFDKLPRESTVFIASQLNQTDKYNLLRSSGYLYTTLLPTLYEKVVVISTSTSINVREIGNGRREFHYPNDKSIVSDKRAIKKFFKTINKTVYNHDGKIRISTLMNLFHCYYSLIPEGFKTFNDDSDKYYTLDDLEDTFFDCFHEAFDFEKTEIPISEFFCLNVFKCLVKLTYCKDICVPSMVYSKLIYNLDHYDYIRDRKVERGVDQTFKNYYKKFLKGELFLDFFRISNGLKLSLSNLTNIRVSFADPFLARSILGSCDSSKLKKLTLDHMCCGHDCESDHLKTLTHDQLNRKFLLIDEFFKLSHIKCIFNRLQDLELVFHNGYNRAVFRKFLFGICHFIVTPNKKFSIIGGKLKLKSLVIRETTSNFTKIREYNHKISDHVSQIHRREVQKSCTLLLFLDLVSISNYFTIFDLIDFTKIEKVDILTKYFPKGTELDAFLDKYFKSFYKNLIVNMKNPLAPNMVTIGIEQYDKSVMVAKAVIHDDESEKDENKVAVVEVEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWL9</accession>
    <name>A0A1E3NWL9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycerophosphodiester phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_85682</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036743.1">
        <property type="nucleotide sequence ID" value="XM_019185929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWL9"/>
    <dbReference type="STRING" id="683960.A0A1E3NWL9"/>
    <dbReference type="GeneID" id="30203175"/>
    <dbReference type="OrthoDB" id="197419at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047389">
        <property type="term" value="F:glycerophosphocholine phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14484">
        <property type="entry name" value="SPX_GDE1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057506">
        <property type="entry name" value="C2_GPCPD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051578">
        <property type="entry name" value="GDPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22958:SF1">
        <property type="entry name" value="GLYCEROPHOSPHOCHOLINE PHOSPHODIESTERASE GPCPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22958">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25329">
        <property type="entry name" value="C2_GDE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SPX" evidence="6">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="508"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="7">
        <location>
            <begin position="805"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="580"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1161" mass="130294" checksum="A942234E91791837" modified="2017-01-18" version="1">MKFGKTITSHQVPEWSHYYMNYKDLKKQIKLITKLQESILKVVEEQDPEIADLQLSDSLNNSQVKSALAGFFFSLDRNIEKVDDFYNKQYSEYDRRLKKISSIINSFNGHIEELDNEELEEIIGVLIELRSCFRNLKWFGELNKRGFVKILKKLDKKTGTSQQNPYLNARIFPLSFANEFDIVKDLSSINDFLNKLSPHIETLNDTKKKSSERRTSGSVTPDKYLRFIQRDDDEPLINQLIVEYRSIVLVPVKLMVSLLNKAALSQSTKCIAKLLEVIPSLGDPSDISGRNFFHHHIIALGKQVQKAEESTEISSKSTENVSALLVGAFGPDGVNSNDSPTSLLYILKNLPPHLRPALLQRDNYKRTPLHYAAQFGLREITTIIIDFLKSWNEWDSTVAIDDLSVWGDAESLSPLHLAVLGTHPKTVEVLIANSDRKLTSSRLFLLAARLNSPEILGSLLGCKGIEIDFADPDTHETALYIASKLNFSKTVEFLLRQKANTEIGENSFGWTPIFIAAVEGLYQIVILLKEYGAEYSLEDESGWTPMEHACLRGHLQISDNLRPKNYDRFQLIENSFKSNNSSTGSVDKVNNSGITSPNSSIDKLSKNHKEDIYKAIKPKHSKQTQPIKSFGHRYLTDDSVILITLGSNDTREGSQAIKLDRVPISKVHSTELDTALSLVVRAKSQFDEPPTVIDLPIDDTHEPITINSNKANPKDEIILFDIVPTYGGQHKQVLGRAVALLDKIYTNVGENRCSLNKVVTIPIIETETLDVLGTLKFEVLIVTPFHHPNMNFNRAETYWKSLVSTRVIGHRGLGKNIDAKKSLQLGENTVESFIAAASLGASYVEFDVQLTKDHIPVVYHDFLVAESGVDIPMHELTLEQFLNLNHVKDDDSKRSKDDDYLMRPRSASHYVYNDGSNEGNSNSDFHLDKMRLTKTYKNLKFKGNSRGSSIASSFVTLKELFKKIPENVGFNIECKYPLLYESQHEELGEISIDLNFWIDTVLQIVYEYGKGRDIIFSSFHPEACLLLSLKQPAIPILFLTEAGTSIMPDVRAGSLQSAVRFAKKWNLLGIVSAAEPIVKCPRLAQVVKASGLVCFTYGVLNNDPDNARLQMEAGVDAVIVDNVLAVRKELTKTNDIVSHETSPEPLGNIDAKAAKAVSVQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWP0</accession>
    <name>A0A1E3NWP0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 subunit I</fullName>
            <shortName evidence="7">eIF3i</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 39 kDa subunit homolog</fullName>
            <shortName evidence="7">eIF-3 39 kDa subunit homolog</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TIF34</name>
        <name evidence="9" type="ORF">WICANDRAFT_34884</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat STRAP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the eIF-3 subunit I family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036763.1">
        <property type="nucleotide sequence ID" value="XM_019182248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWP0"/>
    <dbReference type="STRING" id="683960.A0A1E3NWP0"/>
    <dbReference type="GeneID" id="30199494"/>
    <dbReference type="OrthoDB" id="24966at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000127">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03008">
        <property type="entry name" value="eIF3i"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027525">
        <property type="entry name" value="eIF3i"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877:SF1">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24805">
        <property type="entry name" value="EIF3I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="6"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="48"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="187"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="341" mass="38141" checksum="E0FBACAFAF46D60B" modified="2017-01-18" version="1">MRPLLLKGHERSLTQVKYNREGDLIFTVAKDKEASVWYSFNGERLGTFVGHGGTIWSLDVDNKTELLVTGSADFSAKLWKVSNGEEVYDWKCASPVRRVEFNPQNDKLLAVLDAVMGHKGDISVFKVNHDDYTKQEQEPILVIPTREGFERVTVAGFSYLGKYIIAGHKDGVISKYDAETGEFITSVKIHDGLVTDLQFSDDKTYFITSSKDKTSKILDVDNFNVLKKFESDSPLNTAAITPLKNFVVLGGGQEARDVTTTAANQGKFEARFYHKIFEDEIGRVKGHFGPLNYIAIHPKGTSYASGGEDGYVRVHNFDKSYFDFKYDVEITAEAAASKNQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWS7</accession>
    <name>A0A1E3NWS7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-(5'-phosphoribosyl)anthranilate isomerase</fullName>
            <ecNumber evidence="3">5.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_36430</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrpF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.24" evidence="3"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036224.1">
        <property type="nucleotide sequence ID" value="XM_019182435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWS7"/>
    <dbReference type="SMR" id="A0A1E3NWS7"/>
    <dbReference type="STRING" id="683960.A0A1E3NWS7"/>
    <dbReference type="GeneID" id="30199681"/>
    <dbReference type="OrthoDB" id="524799at2759"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00042"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044643">
        <property type="entry name" value="TrpF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894">
        <property type="entry name" value="N-(5'-PHOSPHORIBOSYL)ANTHRANILATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894:SF1">
        <property type="entry name" value="N-(5'-PHOSPHORIBOSYL)ANTHRANILATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="N-(5'phosphoribosyl) anthranilate isomerase (PRAI)" evidence="9">
        <location>
            <begin position="73"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="232" mass="25553" checksum="122926A9FC253680" modified="2017-01-18" version="1">MLVKICGVKDVEAAQVALESGADMIGMIFVPGKPRTIQVSNAKEVVSLVESKRKGLNSNELYDSLKPGQDSLWFEQVHDSIKSNGPYSVGVFRNQSIEEINNIIEEVDIDFVQLHGQESHDEYIPQLKKPVITRFVPNETKVLQSLKPNKHLLPLFDSEAGGEGVKLNWSNLSDWSAKNNARYLLAGGLTPDNVHEAIKLDGVIGVDVSGGVETNGVKDYVKIKEFVKNALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX45</accession>
    <name>A0A1E3NX45_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial</fullName>
            <ecNumber evidence="5">1.1.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Isocitric dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">NAD(+)-specific ICDH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_97189</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-threo-isocitrate + NAD(+) = 2-oxoglutarate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:23632"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.41"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Octamer of two non-identical subunits IDH1 and IDH2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.41" evidence="5"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036976.1">
        <property type="nucleotide sequence ID" value="XM_019186571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX45"/>
    <dbReference type="STRING" id="683960.A0A1E3NX45"/>
    <dbReference type="GeneID" id="30203817"/>
    <dbReference type="OrthoDB" id="10261637at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045242">
        <property type="term" value="C:isocitrate dehydrogenase complex (NAD+)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000001">
        <property type="entry name" value="Isocitrate dehydrogenase [NAD] subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004434">
        <property type="entry name" value="Isocitrate_DH_NAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00175">
        <property type="entry name" value="mito_nad_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF42">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="12">
        <location>
            <begin position="32"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ57769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="364" mass="39348" checksum="CB4218C8C3DAEC9C" modified="2017-01-18" version="1">MLRAGFKSQSRRTFATAVSDSLLPKKYGGRYTVTLIPGDGIGKEITDSVVEIFKKENLPIDWETINVTGKEGSKEGVREAVESLKRNKVGLKGIWKTDATQSGHGSLNVAFRKELDIYASLTLIKNIPGVKTRLDGIDLVLIRENTEGEYSGLEHESVPGVVESLKIITQFKSERIAKFAFDFAKKNNRKEVTAVHKANIMKLADGLFRSTVKTIGAEQYPDINVKDIIVDNASMQAVSNPQQFDVLVTPNLYGAILSNIGTALVGGPGLVPGANYGREYAVFEPGSRHVGLDIQGKGVANPTAMILSSALLLRHLGLDSSADRISKAVYKVIQEGKNTTKDIGGNASTQEFTKAVIDELAATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXA2</accession>
    <name>A0A1E3NXA2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATPase AAA-type core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_24427</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036807.1">
        <property type="nucleotide sequence ID" value="XM_019181030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXA2"/>
    <dbReference type="STRING" id="683960.A0A1E3NXA2"/>
    <dbReference type="GeneID" id="30198276"/>
    <dbReference type="OrthoDB" id="5925at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051598">
        <property type="term" value="P:meiotic recombination checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044539">
        <property type="entry name" value="Pch2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45991">
        <property type="entry name" value="PACHYTENE CHECKPOINT PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45991:SF1">
        <property type="entry name" value="PACHYTENE CHECKPOINT PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase AAA-type core" evidence="3">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="205" mass="23342" checksum="F19E5D7E63CE73A1" modified="2017-01-18" version="1" fragment="single">PGSGKTTLCKSIAQKVSIQHGKGVFIEFQCSKVFSRFFGESSKNLEMIFKDLRNLIEQNPNLLIIILIDEIETIASSRTNLLKQNETNDGIRVVNTLLTQLDQLKKFDNFLILSTSNNIDSLDSAFQDRCDETFHIKTPNSEAVYQILKQSTNELIDHKIIIHPELLSNSEDDHHNLLKEVSRILYQKGFSGRHLSRLPMMALAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXC6</accession>
    <name>A0A1E3NXC6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosomal protein L32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_80960</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036837.1">
        <property type="nucleotide sequence ID" value="XM_019185155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXC6"/>
    <dbReference type="STRING" id="683960.A0A1E3NXC6"/>
    <dbReference type="GeneID" id="30202401"/>
    <dbReference type="OrthoDB" id="268693at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_eL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018263">
        <property type="entry name" value="Ribosomal_eL32_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_eL32_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L32 AND DNA-DIRECTED RNA POLYMERASE II, SUBUNIT N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="Ribosomal protein L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00580">
        <property type="entry name" value="RIBOSOMAL_L32E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="14991" checksum="977B59B89273150B" modified="2017-01-18" version="1">MAASLPHPKIVKKHQKKFKRHHSDRYNRVAENWRKQKGIDSCVRRRFRGTIPQPNIGYGSNRRTRHLMPSGHKAFLVNNVKDLDLLLLHTKTYAAEISHSVSSKNRIEILARAKALGLKVTNPKGRLALEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXS6</accession>
    <name>A0A1E3NXS6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chromosome segregation in meiosis protein 3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_80161</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037206.1">
        <property type="nucleotide sequence ID" value="XM_019185015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXS6"/>
    <dbReference type="STRING" id="683960.A0A1E3NXS6"/>
    <dbReference type="GeneID" id="30202261"/>
    <dbReference type="OrthoDB" id="437078at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012923">
        <property type="entry name" value="Csm3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07962">
        <property type="entry name" value="Swi3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromosome segregation in meiosis protein 3" evidence="2">
        <location>
            <begin position="35"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="307" mass="35673" checksum="9D0F21B73ADBD5C9" modified="2017-01-18" version="1">MSQDTSAIDSLLGLNLDQPAADQPLPKPRKRINNLNESHFLGIKGLSYIKKNQKQIRSKLNHKDDYKNLTNLLNFYQLWGHSIYPKANFKDFLDMTVKVGSKSKLLKRQRQDWLNQEMGVEKYSSLKNEGLQQDIDNPQDRDNVNNQGSDDEMNDNNNNDDDDDDQVVKQRSKGNRLFVAEDESDDDDDLYAVPKRRVEKLLSQGDNEEIGKDTIAKENTEDKEEEDKQNKDPHTQKKGLQDFNDDFEDDDDDDFDQILEKPAPPPPTQHQPEEDPDAVMRELQQPQPQDEYDEDEIEYEIMREAGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXT6</accession>
    <name>A0A1E3NXT6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_97227</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037017.1">
        <property type="nucleotide sequence ID" value="XM_019186577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXT6"/>
    <dbReference type="GeneID" id="30203823"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="63" mass="7416" checksum="3C5D3A7AB80564BE" modified="2017-01-18" version="1" fragment="single">MYARLHLTLEICHKNKKTKTVSQIHDFGIFEKKNQNAIEKSFWQLSMAIGLLGKFLCFFQKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXY6</accession>
    <name>A0A1E3NXY6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_95292</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037235.1">
        <property type="nucleotide sequence ID" value="XM_019186488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXY6"/>
    <dbReference type="STRING" id="683960.A0A1E3NXY6"/>
    <dbReference type="GeneID" id="30203734"/>
    <dbReference type="OrthoDB" id="10254604at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05243">
        <property type="entry name" value="SDR_a5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15020">
        <property type="entry name" value="FLAVIN REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15020:SF50">
        <property type="entry name" value="UPF0659 PROTEIN YMR090W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="1">
        <location>
            <begin position="9"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="232" mass="25304" checksum="2F35AEA6316EA0E8" modified="2017-01-18" version="1">MVLKVAIFGANGKVATQLLKKFSQLKTEFEPTAFIRNASQGAKFKELGVNYSTDIDITNSNLNTITEALKGYDAVVFSAGAGGKGLDLTFSVDLDGAVKVSEAVKAANVKRFILVSAIKSLDREFWWDGPLRSYYIAKKYADEIISKDDDLNWTILQPGYLKDEPGNGKVEDPKKVEDIADGIPKGDSTKISIPREDVAQAIVEILRDGSSNKKFIPLISGSLEIKEAIKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY32</accession>
    <name>A0A1E3NY32_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_34758</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="1"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037117.1">
        <property type="nucleotide sequence ID" value="XM_019182229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY32"/>
    <dbReference type="STRING" id="683960.A0A1E3NY32"/>
    <dbReference type="GeneID" id="30199475"/>
    <dbReference type="OrthoDB" id="413460at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015616">
        <property type="term" value="F:DNA translocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032392">
        <property type="term" value="P:DNA geometric change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030491">
        <property type="term" value="P:heteroduplex formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045144">
        <property type="term" value="P:meiotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18004">
        <property type="entry name" value="DEXHc_RAD54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000020">
        <property type="entry name" value="DNA repair and recombination protein RAD54B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.850">
        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="261"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="579"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="866" mass="97597" checksum="BBA738DF24A52FDF" modified="2017-01-18" version="1">MNIPSNSRSPSSSSDIAADTNDTHQASEDIKENIQPKLVSKPAAFKSVLPKRSLVAPSISPPLKQQKRESIPGHERFFEVVWRKQTMKKNKTWDGDGFIVLTSNYISLRSDDSSNYKELSRLNNVSKFTTDGVFSMGSYEVEVSSNEINDSQEIEKFKFNARLGNIGATQIQPPLEPIKPDIKKETPKPFKPINTQFKQVVHTEPKPQRGEPLYDPTSEGAIVLRKQHPDDTDVVIDPFLAKKLRPHQVQGVRFLYECVMNQRGFDGHGALLADDMGLGKTLMTITTLWTLLKQSPQGFENPICHKVLIACPVTLIGNWKREFKKWLPMNRLNVLTLNSKNTIAKDKQDVKNFAKTKVYQVLIMGYEKILNMKEELALTKFDLLICDEGHRLKNNSNKTLQALNSLEVEKKILLSGTPIQNDLSEFFNIIDFINPGILGNYNQFKRNFMNPILKSRELNCLNPEIIEKGKEKSQDLIDITKPFILRRTASVISNHLPPRTDVVLFCRPTSLQINLFNEVLSSQNFTSLLDKSSGSSSLGLITMFKKICNSPSLIKNDKLFKEVSDSNIVHQVTSGKILVLTELLKEISAVGEKVILVSNYTQTLDILQNIVLKLNLTYQRLDGSTPNKDRDSIVNLFNNSSSKSNFAFLLSSKSGGVGLNLIGASRLILFDNDWNPSVDLQAMARIHRDGQKKPVFIYRLITTGCIDEKIFQRQLMKNNLSDKFLDNKKGSKDDLFDMNDLKDLFTLNLKTSSNTHDLIECDCGGEGEEISDLEEDDEEEEETAPIAKKQNSWVSALEVKNNSTDEGSERKKTIRKCLADYRHIDPAYLLDGSGDVTTGDEVTEKVVYNSPKLISFILSKVNKPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYQ6</accession>
    <name>A0A1E3NYQ6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">enoyl-[acyl-carrier-protein] reductase</fullName>
            <ecNumber evidence="11">1.3.1.104</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_34139</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 2,3-saturated acyl-[ACP] + NADP(+) = a (2E)-enoyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22564"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9926"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78785"/>
            <dbReference type="EC" id="1.3.1.104"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.104" evidence="11"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037523.1">
        <property type="nucleotide sequence ID" value="XM_019182152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYQ6"/>
    <dbReference type="STRING" id="683960.A0A1E3NYQ6"/>
    <dbReference type="GeneID" id="30199398"/>
    <dbReference type="OrthoDB" id="7482721at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141148">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08290">
        <property type="entry name" value="ETR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000112">
        <property type="entry name" value="Enoyl-[acyl-carrier-protein] reductase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051034">
        <property type="entry name" value="Mito_Enoyl-ACP_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43981">
        <property type="entry name" value="ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43981:SF2">
        <property type="entry name" value="ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="13">
        <location>
            <begin position="18"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ58316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="356" mass="38891" checksum="506ACC56D6777DB4" modified="2017-01-18" version="1">MSTRSITTAKSIIYSEHGIPKDVISVHTHQLPDPKGNQVILKSLGFPINPSDINQLEGVYPSKPEKTLEYGTEKPSAIAGNECLYEVIELGSGVQDLKLGDRVIPLFSNSGTWTSHSIAAEDKLIKLPKDISTVFGATISVNPTTAYQLVNDFGLEKGDWLIQNAGTSAVSQAVAQIAKTKGINVISVIRDRPNLQETIDKLTKQGAAKVITEEQNGDRGVSAEIAKLTEGKLKIALNSVSGKSSSNIARKLAPDSTIITYGGMSKQPVTFPTSLFIFKNLKAFGFWVTENNKKDPDSKRRTVADLVKLYQNQQLEELSVHSNDWIYDEFSDEQIKELIVNALENSSGGKQFITVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYR1</accession>
    <name>A0A1E3NYR1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_95807</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037470.1">
        <property type="nucleotide sequence ID" value="XM_019186503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYR1"/>
    <dbReference type="STRING" id="683960.A0A1E3NYR1"/>
    <dbReference type="GeneID" id="30203749"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000048">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="94"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="349"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="431" mass="49556" checksum="685BDB4524E88E0C" modified="2017-01-18" version="1">MPNSRKLSGLYIANEDKLKFTAIADSDDDSDDDNDQFLDPNSFNIPSGRKFSTISRRNSVRSKISINGSAKDSTNIPITQDYSSDLSQRTINDFKEINILGIGSYGRVYLVEDVYTKKLYAKKTIKKAKISIDEKLFKTHKNEREILSSINHPSIVKLFYSFHDYQDINFILEYIPGGEIFYHLSKTKRFNEFDTAFYLAEISQALTHLHKVAGVVYRDLKPENVLLNAQGHVVLTDFGLSSLDEVCHSILGTPQFTAPEVLKGEEYSYPADWWSFGIMMYDMLVGETPFSGNNRAKIFNKILKTKLNIPDYLSEDAKDLLRKLINKKPEKRLKIDSDFENLSNHRFFRKIDWENLRNLKPPIIPDTTDISKANNFDHGYINLILAKEEKILDNVEAENDQSKNDETDLFDNELFNGFSFVASKNFLDTHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYR2</accession>
    <name>A0A1E3NYR2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear segregation protein BFR1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_80496</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037558.1">
        <property type="nucleotide sequence ID" value="XM_019185074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYR2"/>
    <dbReference type="STRING" id="683960.A0A1E3NYR2"/>
    <dbReference type="GeneID" id="30202320"/>
    <dbReference type="OrthoDB" id="2195113at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042175">
        <property type="term" value="C:nuclear outer membrane-endoplasmic reticulum membrane network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039604">
        <property type="entry name" value="Bfr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31027:SF2">
        <property type="entry name" value="LEBERCILIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31027">
        <property type="entry name" value="NUCLEAR SEGREGATION PROTEIN BFR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="468" mass="52678" checksum="2632DB7856E4081F" modified="2017-01-18" version="1">MSSDSFSATSNEPRVQRRFIKSPDSKVRDDTLAKINQEIKQKDIALAEINAQLKKAVTDPKVSNERKTLIGELNELRKTQADFKGKRDLINNKIKEIDASLKRKIGEVQATTSKNNFKSAEEIDARIKKIDDNISSGDMKLVEERLAIKEITSLRKLRKDFSGIQTQQKSIDADKEKIAALKKELSGLNSKEVSAKYESIQKQLDELNLDNKSVNDKRTSLYNKRNALQKEKDALYNSIRKTRADFDEQYKHFKQALADERKRVADEEAKLKAEKEKSERSSKTSKILAEATQPAFEEEIDTVHTLLNVFDPSYVKPTKSNKSALEKSTFVTERQGRVIEQSTEDEIIKKDDNLFFAGSGPHKSSKKGKKGSKKFTLEPDVISSLGDLEIPLPLSQDDVPQTVEALKSKLQTFTATQEETTKKNIEAAKEKIAKLEAGWAKQDAKEAEAVAKAEAKKEAESKEAADQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYS1</accession>
    <name>A0A1E3NYS1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_64404</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037472.1">
        <property type="nucleotide sequence ID" value="XM_019183728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYS1"/>
    <dbReference type="STRING" id="683960.A0A1E3NYS1"/>
    <dbReference type="GeneID" id="30200974"/>
    <dbReference type="OrthoDB" id="10264848at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045046">
        <property type="entry name" value="Vps9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101:SF25">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN AND VPS9 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101">
        <property type="entry name" value="RAB GDP/GTP EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00167">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="2">
        <location>
            <begin position="160"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="417"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="472" mass="54858" checksum="CF55E7316625D953" modified="2017-01-18" version="1">MFHTPPIDSSFKDESTKESYSDPLHTIINQQPADNNEGNDNDDEGLPQELPPQLNELITIFLKDLKTPKYNKPLTPEQLYSTFQTFYKKFHQRAELFVKGSVYYKKRITRIELQTRNEIASDKKFNKTIELKIRRYIEIAEEQICIELFEKLFNKFPKDIKINSYLIEKLSIIKKIKGIDYNVLLDIAKIEFDQPIIKEISHKFNELNHFTTPCSKLKVLISIHSSINQFLKTQLSNEFVDGDYFLPLLIYLILINEDDVDFYSNFIYIKRFRQDSLLVGESLYCLTNFEAAITFIQHLELLNDGFDYSNLTQHELDFLKLKLSLEIDESIDIHIPSDGHILSNSTDGIKLISSAIDSSFKSMMHRFGSSSIVPTQTNEMIDEVEPSNDGYNPINKLVGVMKWRSNTPTPPDNGTVTNRSRSNTLNRSRSSTLNSTPVDIHKFKNRKFKDLKISELEELFNDYSTLTKWLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ04</accession>
    <name>A0A1E3NZ04_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_80590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037658.1">
        <property type="nucleotide sequence ID" value="XM_019185088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ04"/>
    <dbReference type="GeneID" id="30202334"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="225" mass="25997" checksum="EC31322CA10E263E" modified="2017-01-18" version="1">MRMNQRINQVYEVNMMLVKRRNELENELENELIGNLSLMSNSISTAEVSQYSLNASCIFTIDQVLDYQIEDLGNTKGKLVLLEDIKIKNANELFNDILADFNSLLYEDKLNRKKHLRIPNVILMKDCEDANEQELDITAFESSTTSLCPYLKASTQQILDGKVIDGNLKREDIINNIHDSLNSTFNITITIKKNQFKNNVLKQIHATKFEQVNRNKKIKLEINEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ12</accession>
    <name>A0A1E3NZ12_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Allantoin permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_34340</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037633.1">
        <property type="nucleotide sequence ID" value="XM_019182173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ12"/>
    <dbReference type="GeneID" id="30199419"/>
    <dbReference type="OrthoDB" id="2018619at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11482">
        <property type="entry name" value="SLC-NCS1sbd_NRT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4160.10:FF:000001">
        <property type="entry name" value="Uracil permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012681">
        <property type="entry name" value="NCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00800">
        <property type="entry name" value="ncs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF2">
        <property type="entry name" value="ALLANTOIN PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="398"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="478"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="508"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="575" mass="63879" checksum="C17BCE3935DBCB31" modified="2017-01-18" version="1">MIEDVNSKEKVSIDAETTTTIESNTRDDNSNVFKRFLNKISLQDDDLTLSESFLINKDLQPVFDKTERPWRWYNFVFLWVAESFNINTWQISATGIISGLSWWETWISVWIGYSIVGGFIFISQRVGSHYHISFPVSCRTSFGTFGSIWPIVNRVVMACVWYSVQAYLGSQALSLCLKAIFGSDLETRIPNKIASSGTSSFMFLCYFLFSLISLPFIYCKPQKIRHLFTVKAYTCATAGVGFLVWTIVRAGGIGPVIHKETSLSGSAHAWAFVISTMNCVANFATLVINSPDFSRLAYSPQSSSISQLVAIPASFSITALIGILSSSASTTMFGETYWSPLDLLDRYVQAGTPGDRAGVFFIAAAFVLAQIGTNISANSISAGTDGSALLPRFINIRRGGFVCAAIAFCICPWNFFTSSANFTTYLSAYAVFLSSVAGVVAADYCVVRRGYINVFHLYSNNSEYHYSYNRFGINWRGFVAYICGILPNVVGFAGAIGRKVPIGATYVYNVSFFSGYIVAFVVYCALCWVSPVPGIPEKKFLTKRGWFEQNVNYDVDNFFEEMGKPDDFIRTDTKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ43</accession>
    <name>A0A1E3NZ43_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Probable metalloreductase AIM14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_33741</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Probable cell surface metalloreductase. May be involved in iron or copper homeostasis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ferric reductase (FRE) family. AIM14 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037673.1">
        <property type="nucleotide sequence ID" value="XM_019182109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ43"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ43"/>
    <dbReference type="GeneID" id="30199355"/>
    <dbReference type="OrthoDB" id="17725at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000293">
        <property type="term" value="F:ferric-chelate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033215">
        <property type="term" value="P:reductive iron assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050369">
        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF198">
        <property type="entry name" value="METALLOREDUCTASE AIM14-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972">
        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00463">
        <property type="entry name" value="AIM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Ferric oxidoreductase" evidence="16">
        <location>
            <begin position="110"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding 8" evidence="17">
        <location>
            <begin position="270"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Ferric reductase NAD binding" evidence="18">
        <location>
            <begin position="360"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF08030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ58466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="526" mass="61260" checksum="22E80F84DF5B5C57" modified="2017-01-18" version="1">MVKFTNSSSLDYKLDQFLVVTEDNIQYRYYTLGICFAHALLIIINTVLVRPRLSSIKRKSVWWRTFKSLIDPPLWIQVSIWIIIAVLINVIPLTSTSRTELDWVIFWKRTGTVGYSLTPFILLLSLRPNPLPQVLYANLLPLHKWLGRIYICLICVHSVYFIQQWGRSSRFSELFWKVNPITGFISFVIWLIFALVSLRPFRTRLYKVFYCLHMIAIWTTVPLIGIHSRPRTHFINVVNLVLMIYMGFSKIYYYAVINKRLEFLDIERHINSQLIVLTLPKSYYRGKQLRFNPGSHVRINHRFINPLSWISPSHPYTIASLDTDQEIKLIIKETKFKIHSLGRYSISSPFNNETGTIHGYDNITIICGGSGIAFGLPFYRMNNPDCFVHLVWLVRCIEDLHVLLDIYITCNEESFEPVQEIQQDDDNIISMMSLNELFEWNDGDYTEMKEDDVVELQEMGISTMDTSCCFIHHEGRPSLKAIVNEDFQTGTCTNCLVACGPSRLVKECSNLAKLYDSEFISETYNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ47</accession>
    <name>A0A1E3NZ47_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_96319</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037656.1">
        <property type="nucleotide sequence ID" value="XM_019186514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ47"/>
    <dbReference type="GeneID" id="30203760"/>
    <dbReference type="OrthoDB" id="272985at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="53" mass="5742" checksum="E324A5CCAFEE7ECD" modified="2017-01-18" version="1" fragment="single">MSHKKDLEYINVGLKIANETNDHMGGKTIIFSGDIKQLPPVSTSNTSSISPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZB2</accession>
    <name>A0A1E3NZB2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_34671</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037710.1">
        <property type="nucleotide sequence ID" value="XM_019182216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZB2"/>
    <dbReference type="STRING" id="683960.A0A1E3NZB2"/>
    <dbReference type="GeneID" id="30199462"/>
    <dbReference type="OrthoDB" id="276546at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000138">
        <property type="entry name" value="NADPH dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF91">
        <property type="entry name" value="NADPH DEHYDROGENASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="4">
        <location>
            <begin position="21"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ58503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="406" mass="45536" checksum="2B92D2BBAD680F51" modified="2017-01-18" version="1">MTLAQPDYDQRIQPLSLKDTNLFKPIKLGDIEVSHRVVLAPLTRLRADPDSYVPNVELASEYYRQRSENPGALIITEGIVVSKFTTGFFDGCPGIWNEEQTEAWAKIFKPIKDNGSFVVAQLFGLGRQAIPDVLAKNGIPYKTATAGRYITHDGAYDFKEKALEFNNPLLELTLEEIEQHIVDFIQAGINCIKAGADGVQVHVANGYLLNQFLDPESNQRTDVYGGSIENRSKLPLKIIDGLIDAIGAGKVSVRFSPYSKFGGFNGVQDDPTLLAQYAHIVGELEVRAQQGKRVQFLDIVEPRIFTGVSTDEYPEFKGGNNDFFYHIWKGPIVRAGDYMSNHDALKNDIQDGRTLIAFGRYFISNPDLPTRLQKGLPLNKYYRELFYSPGPKGYIDYQKHSETLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZD6</accession>
    <name>A0A1E3NZD6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_101596</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037567.1">
        <property type="nucleotide sequence ID" value="XM_019180326.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3NZD6"/>
    <dbReference type="GeneID" id="30197572"/>
    <dbReference type="OrthoDB" id="5600252at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008186">
        <property type="term" value="F:ATP-dependent activity, acting on RNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23827">
        <property type="entry name" value="RWD_YLR419W-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056328">
        <property type="entry name" value="DSRM_DHX29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059023">
        <property type="entry name" value="RNA_hel_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF267">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE YLR419W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24385">
        <property type="entry name" value="DSRM_DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26026">
        <property type="entry name" value="RNA_hel_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="6">
        <location>
            <begin position="407"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="581"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="794"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1393" mass="157034" checksum="7C1E9E5991E8FC17" modified="2017-01-18" version="1">MAKKPSKQSQQPPKEAPSNDAKQKGKKNEKANSNNNKSSSSNGTASTPASTARPSSGKTPINLLHEHAQRSKWEKVQYDMFKVKDGFVASAILQWKDPKTNEFIDVKITHKLPSKETPIEARYFAATVALHRVCFNKNLHMVLPREFKDVWNELEDQRKAIWKENSEKHNKLYNNEPFKVVLEERKLQAKKSKEAQVKLNNEAKVKKTPVVLTSIKSSVPSTAKLPKFKATAESKVTKPQTSKKSTSAVSFPRKVWETALSFTFTVEQRDLIQKSIKSHINWQKTTKNESEMKSLLLSIGFRPSHVDESLQYQDPLSFLLFNVPEDDLPFFFQDSTSSDSITVAEKNEGLVRKIMNFGVGRSEAIMALNGNGQDLQKTIISLTQKLVNYRGNQDFIDPIESQDLWNEEIGSLSSIYEPSKIIVLDESSVQINFNSFLCLRVYKSPCYPNDLAGLMISTIDENHKLPNYVKLNLVKGMAHYAIDNALGISYIYSLVDWLQENSERIIESPGLLLESSDIAQSTKDLNIDSQNSSTQRRFFKSNPDINFIKNDYEKRTKTVELKNSIKARTKLPAWNEQENLLKIINSNKVSLITGETGSGKSTQLVQFLLDDLYSKGNYHTQIFCTQPRRISAIGLAERVAEERASVCGEEVGYIIRGANKSNKNTRIKFLTTGILVKFLQNGDDFLNNAILVIDEVHERSMETDLIIILLKKLLNKYKDLKIVMMSATVDLSVFQNFFKGLSSAHIKGRTFPIEDFYLDDVLQKTDFQIEMNDEWISPRADSKFFQTGNVNYDLITSLVSEIHQDLIDESNDGSILIFLPGVAEINKCVKLLNQNFNPPSVILPLHSALSPQEQHRVFETFPGKRKIVVSTNIAETSITINDCVVTIDSGRVKSMSYNSIDNTTRLVEIFESKAEAKQRRGRAGRVSNGKSYKLFTKETYDSMMDSPIPEIKRINLESLYLVVKSMGIKDVIAFLNTGMDPPPLNSLIKSEELLKCAGLVNEYDDLTELGKFISLLPIIDPKHGKLLIFSIIFGCTDIGILTASVLSVGTPFIKSADLRDEIKQTLSKTKDLGDLLSNVIIVDQYLKLHSPSEKKQFMNKNYLSFNKINEIKSAKTQYLSILEDIGFLPLKYKEGQSHLNRNSSNYNVIKAIICGSFYPQVARVQLPDPKYLNTSSGAVQVDPEAKATKYWIRNEEYISALNRSGVVEDEKLPATRAFIHPSSVMFDTNSNSSTPQSGIPQDLKSDREDGLIEFEKIQQMRPKDFTPTLRNAKSKALKSSFIVYNSSTVTSKLFLRDITPTSTLSALLFGGPLSYDLSSISSGRQSPGIVLDNWLPIKTWSKNAVLIKELRLLLDQVIKLKLEDPSYSSNKENKKALELTSRLKLFESTIGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZE1</accession>
    <name>A0A1E3NZE1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_84357</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037766.1">
        <property type="nucleotide sequence ID" value="XM_019185699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZE1"/>
    <dbReference type="STRING" id="683960.A0A1E3NZE1"/>
    <dbReference type="GeneID" id="30202945"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015190">
        <property type="term" value="F:L-leucine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903801">
        <property type="term" value="P:L-leucine import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF26">
        <property type="entry name" value="GENERAL AMINO ACID PERMEASE AGP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="459"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="40"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="508" mass="56553" checksum="901EECCE459A66F4" modified="2017-01-18" version="1">MDSGKEQISDNVSVVETSPVGDDILDNGLNAGLKNRMINLIALCGVIGPGVFIGISNALASGGPVGLLVGFSIVGILVISMMFSIGELNAAYDQNFAILASRFVTRGFGATVALYYVVVWITNIISEYTSLTAALQVYSTKIPTYGWFLIFWLVFTIFQTLDVSWWGESEYVLGVMKLMFLTGFYIFAIVYAGGGIKDHKPDNPFGNFPLRSGFKGIANSFVYAGVYYSGVEGVSMLAGESRNPRKAIPVAVRNTFIRVFYVYFGLSIFYGITVAYNDPMLTSGDKVLKSPMTIAFTNAGWANGKYFVTTMIWITCFSSINSAIFFASRALFTWAKEGYGPKIFTKVNKRGIPYVAIHFVHLFGFLSILSYKSGSSVAYNYIVSVTGVASFIVWTCISITHLRFRKAYIKNGLPLEELPFLAPLFPWSNYIAITLGIVLTLVQSWSCFVPFDYKTFIDGYIMLPLFFIVWFCYDFFYFKNGFIKFNEIDFDSGRRPDLDDLPEKLSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZF5</accession>
    <name>A0A1E3NZF5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_93043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037803.1">
        <property type="nucleotide sequence ID" value="XM_019186395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZF5"/>
    <dbReference type="STRING" id="683960.A0A1E3NZF5"/>
    <dbReference type="GeneID" id="30203641"/>
    <dbReference type="OrthoDB" id="3980283at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="230" mass="27509" checksum="9E19B476A110A843" modified="2017-01-18" version="1" fragment="single">MYSLLEFRPSRINPNNWLLNELILKDFQIEEDEEVFIEKTNGENFVLNHGYNNNHQEDYSIFGNLIFVKMVHLSYHINDVLNDVFNLKSLKFDDFDTILIKSSRLLINLDEWFENLPLQLKNSTILNNQITSAPLILLFYTVKFLIYRRLINHFIETSTSNEQHYEKFKPIFKKLFLEISKFNENFINNLSIQHFTNCFWYSNTTKSFITVAIVNLLLIKTTRDEIYLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZK5</accession>
    <name>A0A1E3NZK5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCA7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_24108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037244.1">
        <property type="nucleotide sequence ID" value="XM_019181016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZK5"/>
    <dbReference type="STRING" id="683960.A0A1E3NZK5"/>
    <dbReference type="GeneID" id="30198262"/>
    <dbReference type="OrthoDB" id="21678at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904802">
        <property type="term" value="P:RITS complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013243">
        <property type="entry name" value="SCA7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037804">
        <property type="entry name" value="SGF73"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47805">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47805:SF1">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08313">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51505">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCA7" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="50" mass="5235" checksum="C611EB31D6056B2D" modified="2017-01-18" version="1" fragment="single">KQKGPVDVEKQCGVALPNGGFCARSLTCKTHSMGAKRAVPGRSASYDTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZL5</accession>
    <name>A0A1E3NZL5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_34520</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion inner membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037715.1">
        <property type="nucleotide sequence ID" value="XM_019182198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZL5"/>
    <dbReference type="STRING" id="683960.A0A1E3NZL5"/>
    <dbReference type="GeneID" id="30199444"/>
    <dbReference type="OrthoDB" id="1551503at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042719">
        <property type="term" value="C:mitochondrial intermembrane space chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140318">
        <property type="term" value="F:protein transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000008">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit TIM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050673">
        <property type="entry name" value="Mito_inner_translocase_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13172">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="14">
        <location>
            <begin position="14"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ58508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="87" mass="10561" checksum="49E8BB9486DF3221" modified="2017-01-18" version="1">MDQLNQREQQDFQRIVEQKQMKDFMRLYSNLVERCFTDCVNDFTSAKLTSKEETCVMKCSEKFLKHSERVGQRFQEQNALLTEQMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0D9</accession>
    <name>A0A1E3P0D9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Non-structural maintenance of chromosomes element 1 homolog</fullName>
            <ecNumber evidence="4 15">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_69136</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the Smc5-Smc6 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the NSE1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4 15"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037927.1">
        <property type="nucleotide sequence ID" value="XM_019184157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0D9"/>
    <dbReference type="STRING" id="683960.A0A1E3P0D9"/>
    <dbReference type="GeneID" id="30201403"/>
    <dbReference type="OrthoDB" id="185455at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011513">
        <property type="entry name" value="Nse1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014857">
        <property type="entry name" value="Nse1_RING_C4HC3-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20973">
        <property type="entry name" value="NON-SMC ELEMENT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20973:SF0">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07574">
        <property type="entry name" value="SMC_Nse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08746">
        <property type="entry name" value="zf-RING-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="13 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Non-structural maintenance of chromosomes element 1 RING C4HC3-type" evidence="16">
        <location>
            <begin position="202"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ58720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="268" mass="30259" checksum="0E741815A2166A8A" modified="2017-01-18" version="1">MSDFIYTNVERALLQYIISVGSAREESLTSVHTKLKQTDDEDLALIDGLKERRLDETIEKINDKLHLLGYEIAKAKSQETQEINYVFINTIADAPAKISTTHSPKEIEVIKKIIEKIIVECEDESFVITSSEALKICSRSAQLSASEGEHLLRELVNEGWLNRSNKGRYSLSIRALTELKRTLIDKYGIRSRTSPEGHVTLCTGCNEIVTSGVRCFSDSCFIKFHSECQGHYFRAKGTKCPNDECTVDWAEDGPRPVGEKRLRSKLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0F5</accession>
    <name>A0A1E3P0F5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_64541</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ELP6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037609.1">
        <property type="nucleotide sequence ID" value="XM_019183758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0F5"/>
    <dbReference type="GeneID" id="30201004"/>
    <dbReference type="OrthoDB" id="9995306at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19495">
        <property type="entry name" value="Elp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018627">
        <property type="entry name" value="ELP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16184">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16184:SF6">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="30021" checksum="46796D28277C8A3B" modified="2017-01-18" version="1">MAQKQDLNLFIDNSIIPEKVFTSEPLFGTITHTQGTSPSWLVNLLIENSLYGTCVINHEKDQHRQIRADTVVISFLHDYDYYEKDLKRNGIELNDNKQFHFIDLFTDLFQEISTPESIGKLFDQIATQIQGYQNTKKIILIEGIEFLLSATSISTIQLLNQINKLNKLAKGIFVITSADKELIEASQGNQNLPEFKTLDFIVRLIHRSNIVMGLRPLETGRAKDITGTLTISKGTIPFESLVVSEKEYLYFINRETTKLFFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0L2</accession>
    <name>A0A1E3P0L2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_79553</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038223.1">
        <property type="nucleotide sequence ID" value="XM_019184912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0L2"/>
    <dbReference type="STRING" id="683960.A0A1E3P0L2"/>
    <dbReference type="GeneID" id="30202158"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000017">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004762">
        <property type="entry name" value="Amino_acid_permease_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00913">
        <property type="entry name" value="2A0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF13">
        <property type="entry name" value="HISTIDINE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="121"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="442"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="489"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="522"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="10">
        <location>
            <begin position="93"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="595" mass="65982" checksum="06EA668572EBC6E3" modified="2017-01-18" version="1">MSDADSKRASGITVNTSNNPIAPNNDVFTDSNEVPGSSRWQNFKNSFKEALPETSDSYIDKTELKDYNELTDVEKAAFNSSKTQMKKDLTFRHLMMIVIGGALGTGLFVNSGGSLNKGGPASLVIGWVIIATMMFSTMVALGELCVTFPVTGGFTTYATRFVDPSFGFAMGWNYALQWLILLPLELVAAAITIKYWNHTIDSDAWVAIFYVFIFVLNMLDVKYYAESEVVLSLIKIIAAVGFFILGIVLICGGAPEGGYIGGKYWHDPGAFSHGFKGVCSVFVTAAFSFAGTELIAVMGSECSNPRETIPKAAKQTFWLITIIYVTMLTLIGCLVPYNDPRLLNSDNGDYASISPFVIAIQNGGIKGLPSLMNVIILLAVLSVANSSVYASSRVFASLADIGHAPKFFSYIDRKGRPLVATLFTLIFGLLSFIASSDKQDEVFTWLSALSGLSTIFAWLSICISHLRFRRALYVQGRSTDELTYVSQTGAWGSVYGIVLNVLVLMGQFWVALFPTNKADAKSFFEVFLSLAIFIVFYFGHKLWKRNWILFIRAKDMDIDTGRKHIDMELLKQEIIFEKQRLANSPVYYRLYKYWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0M2</accession>
    <name>A0A1E3P0M2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_23350</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038100.1">
        <property type="nucleotide sequence ID" value="XM_019180985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0M2"/>
    <dbReference type="STRING" id="683960.A0A1E3P0M2"/>
    <dbReference type="GeneID" id="30198231"/>
    <dbReference type="OrthoDB" id="17948at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000171">
        <property type="term" value="F:ribonuclease MRP activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034965">
        <property type="term" value="P:intronic box C/D snoRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000294">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, RNase MRP-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002738">
        <property type="entry name" value="RNase_P_p30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01876">
        <property type="entry name" value="RNase_P_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="247" mass="28063" checksum="D4C7CB6DA5CAFE1A" modified="2017-01-18" version="1" fragment="single">MLIDLNIHWPQKNYQTPLSHNDIVKLKNTLVTLEELGYTHIALNYTLNHSDKIPSNQTINPINLDLLKEFQNRLKIYTRVTIIVDEPSQGQSISKLQNGFDIVSVLPISEKGLLLACNSLDIDLITFDYSKKLPTFLKQKTIGAAIKRGVKFEITYSNFLKNENRPQFINNTLNLIRASRNQNLVVSSGAQQSVELRSFPDVLNLLQLLNFKQSDGNNLIDKASKVLLNGRLRIKSYKQTVMIGEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0N9</accession>
    <name>A0A1E3P0N9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_63547</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038253.1">
        <property type="nucleotide sequence ID" value="XM_019183569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0N9"/>
    <dbReference type="GeneID" id="30200815"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="53" mass="6148" checksum="18C4D408365B51FB" modified="2017-01-18" version="1">MLLKLDLSAIQLRDCAILMNCTDTDVDYDTPITKFDVSLLFILRYSYETEVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0R2</accession>
    <name>A0A1E3P0R2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_70420</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037714.1">
        <property type="nucleotide sequence ID" value="XM_019184204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0R2"/>
    <dbReference type="STRING" id="683960.A0A1E3P0R2"/>
    <dbReference type="GeneID" id="30201450"/>
    <dbReference type="OrthoDB" id="3981115at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15398">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15398:SF4">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN 8 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="5">
        <location>
            <begin position="385"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="121"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="121"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="192"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="217"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="258"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="289"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="479" mass="53504" checksum="9EBE0E30A8BCAC61" modified="2017-01-18" version="1">MSIINNNPLLQLVRTHISPDKTIETLPKVTIRDVILSITKIFQSSDIQILANNDNFKIKLSREDFQGLIDKNYGRYRDNAIESIRDYEEDYEKVMNEISMIEKGELDQLVIQEYKQSLPAQLTKPAQQQQQPSNTPAQQLKPQVQAPPSIIQQQSQTQTQAPPQLQPQQTKQVQAVHQTQASTQSQPPQATIPQTQSVPVPSTRLQATNAIEEPEKSNISVSKTDNNLPPNPVKSVDFAPVSNGEENEPVPATMENGNAEEETEEPEEVKAPTPEATTPPGDDNDVEMVDVKEEVETEKEGVKEEELIGETSDAKESTDGSEGDDRSVESARKRRSSTSKLEDKTPAKKRAKLSTSVPSSPAGPVHAPPNKKLQILSNPLIANITSYKYASTFLQPVSESNAPDYYSLIKEPRDLKTIKQMVKDGRIQNAAELEREILLMFANAIMYNKDGTDINNWSKEMQIETDKLLQLFKESYDTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0T0</accession>
    <name>A0A1E3P0T0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">5-formyltetrahydrofolate cyclo-ligase</fullName>
            <ecNumber evidence="5 7">6.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_80661</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>(6S)-5-formyl-5,6,7,8-tetrahydrofolate + ATP = (6R)-5,10-methenyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10488"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.2" evidence="5 7"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037734.1">
        <property type="nucleotide sequence ID" value="XM_019185106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0T0"/>
    <dbReference type="STRING" id="683960.A0A1E3P0T0"/>
    <dbReference type="GeneID" id="30202352"/>
    <dbReference type="OrthoDB" id="2015992at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030272">
        <property type="term" value="F:5-formyltetrahydrofolate cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009396">
        <property type="term" value="P:folic acid-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02727">
        <property type="entry name" value="MTHFS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407:SF1">
        <property type="entry name" value="5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407">
        <property type="entry name" value="ATPASE INHIBITOR/5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006806">
        <property type="entry name" value="FTHF_cligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="15"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="164"/>
            <end position="172"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00036539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="226" mass="25438" checksum="AC9C26FBD82EDDE8" modified="2017-01-18" version="1">MSQLNAQTLAIVAGKKLLRKQIMSKLSSLPKNVIQAQSLESFNILKNTKIFKEAKSIGVFMSMDTEIKTSYILKEAFDCGKDVYLPRIETQSQDQITLYKNGPHYVMFYQMDNYNSCINLPQVGKYKLQEPSGGVCLLNDHPYGDKGLDLLIMPGVAFTSDLNRLGHGGGFYDDFLKRYKDTYGKHPFLLGIGLGDQLVDEIPTDIHDEKLDGLIIRDKLYGNDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0V8</accession>
    <name>A0A1E3P0V8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_17192</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037764.1">
        <property type="nucleotide sequence ID" value="XM_019180731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0V8"/>
    <dbReference type="STRING" id="683960.A0A1E3P0V8"/>
    <dbReference type="GeneID" id="30197977"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03048">
        <property type="entry name" value="GST_N_Ure2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF8">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE GSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="92"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="23327" checksum="EB0D9D14DACE1251" modified="2017-01-18" version="1" fragment="single">LKFYSAGTTNGQKVALLLELLGLKYILRPVDFRKTKENKQEWFLKINPNGKIPAILDVDSEGNTITLAESGALLLYLAEKYDVNHKYSYASTDPLHWEEVELLFFHASGLNPAQANLNWFRNNSPEDAKNIERFNDEVIRSYQILDDKLASNGSGFLVGDHVSLADLIALPHARVLSNSGIDVSQFKNLEKWVAKVSDIPEVKKALAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0Z8</accession>
    <name>A0A1E3P0Z8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_63652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038363.1">
        <property type="nucleotide sequence ID" value="XM_019183592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0Z8"/>
    <dbReference type="GeneID" id="30200838"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="263" mass="30695" checksum="DD8C24C795B77D54" modified="2017-01-18" version="1">MKYTQVKEKIEYLINCLNEDESYILAAQITQLLTLIELNFGINIDAKIHEVSNLFINQYYDVAIRKVLLLDFINGLVELDIIALVNEMVNEDSSSNIGKVDKKYPLVTSSPLPKENNDTISLQEKQLKDLDDHINSRISSLGNSFNESPKPYRPENKNTSTCLIFFTILFNRIYSLLITPNQLTLVLNLILVSLLSITVTFQNFEVPFNIYNLTYKLFIYPTSKDLYWWQKIPYTEQKIWGFLDYIKFKDQNFTMDTKRTLIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P120</accession>
    <name>A0A1E3P120_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_31808</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038157.1">
        <property type="nucleotide sequence ID" value="XM_019181883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P120"/>
    <dbReference type="STRING" id="683960.A0A1E3P120"/>
    <dbReference type="GeneID" id="30199129"/>
    <dbReference type="OrthoDB" id="21225at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000146">
        <property type="entry name" value="Putative two-component response regulator SSK1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="350"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="216"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="266"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="217"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="270"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="301"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="3">
        <location>
            <position position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="542" mass="60138" checksum="346443F29E8DD246" modified="2017-01-18" version="1">MSKQGVKYSSPTKQQQSYRAGSPQIPTINTSRETLTRRIWIRRPEGNPTSLYVKETDLIDDLKTLIMQKFPLTLSKACDPADLIIRLEPSLGTNSPSYFLSPSNPKFPQPISAGANSASSAGYSPSFNSPISPIPVSATKHSSLIPQKPPTLNDFSNLSIKSNSPINIALEADRPIWNILDQYYPNGMSMDNAFIIDVPHSHLIKDDEIAYLHSSRRMNSLPTQSPLSIRSTPTSYHRRSQSNPPDHSSAVLLLPKDFSALNETVRKANNESSNSKSDSVYSLDRTISPSTSDTTLHPTMSQQAPLLPQTSSSSPQHAAPLTPFPRAESANSMKKKDENSTMAKVLPSVNVLIVEDNLINLRILSAHLRRHKIHYDIAKNGQEAIDKWRTGGFHLVLMDIQLPVMSGLGASKEIRRLEKINRIGVFANSDLQNMEPKKPEDQLDLNVFRSPVIIVALTASTMKQDKQQALAAGCNDYLTKPVNLDWLLNKITEWGCMQALIDFDGWKSGERMTNISAAIPTLTRKRSSSRRIHSHEISAQGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1K6</accession>
    <name>A0A1E3P1K6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_23571</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family. DCP2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038337.1">
        <property type="nucleotide sequence ID" value="XM_019180995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1K6"/>
    <dbReference type="STRING" id="683960.A0A1E3P1K6"/>
    <dbReference type="GeneID" id="30198241"/>
    <dbReference type="OrthoDB" id="18996at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140933">
        <property type="term" value="F:5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03672">
        <property type="entry name" value="NUDIX_Dcp2p_Nudt20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000003">
        <property type="entry name" value="M7GpppN-mRNA hydrolase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1050">
        <property type="entry name" value="Dcp2, box A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007722">
        <property type="entry name" value="DCP2_BoxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036189">
        <property type="entry name" value="DCP2_BoxA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044099">
        <property type="entry name" value="Dcp2_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114:SF17">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05026">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01125">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
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        <location>
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            <position position="1"/>
        </location>
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        <location>
            <position position="186"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005279"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59130.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="186" mass="21569" checksum="CA95B9C1E6378C7C" modified="2017-01-18" version="1" fragment="single">VLEDILVRFVINAPPVDLSDNARVFFLFEEAHWFYLDYVRSINPYLPNLKMKAFSKKLIELFPLIWNGGDPDEGLKEFGNYKQSIPVRGAALFNESLSKILLVQGVESPNWSFPRGKISIDEDDVTCAIREVKEETGFDITSYINKDDYIERTIRAKNYKIYLVKNIPEDAEFKPIVKNEIKEIGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1W7</accession>
    <name>A0A1E3P1W7_WICAA</name>
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        <recommendedName>
            <fullName evidence="7">D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="5" type="ORF">WICANDRAFT_32527</name>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <text evidence="2">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
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        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
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    </dbReference>
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        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="4">
        <location>
            <begin position="189"/>
            <end position="336"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ59184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="40892" checksum="C8B41CE5DC2F50EF" modified="2017-01-18" version="1">MTKHKVLIPIHPDKCDLADHLPEFKKFQQTFDVHIEVLTTSENFIHALKTKYNDIAAVWATSSLYKLGGLNPFIDYLPDTVKVVAFPWVGHNAFDGKKLKERGIILANIGDSPSKDVADIALLLTLATFRYSSYLEHALRTSDASISEARDSFGSDSFDPKTGEPIPPEDIKHKNLAKKLTIGGKTLDSPANKVAGLVGLGSIGKEIAKRLDAIGMSIKYTKRTPLKPEEEEALPFKAEFYSSFEELIPHVDLLVIAVPHSESTINLINKDSVKLLKKNVRIVNIGRGTAIDEDILLEALDDGTVNSVGLDVFQNEPKIDKRFLNRWDVVILPHIGSFTVDNFRDANIRVIENIEDVLLHDGPSLSPVN</sequence>
</entry>
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    <accession>A0A1E3P284</accession>
    <name>A0A1E3P284_WICAA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">WICANDRAFT_100030</name>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ59581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="30197531"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="797"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
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            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
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            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
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            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
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        </location>
    </feature>
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        </location>
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        <location>
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            <begin position="738"/>
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        </location>
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="EMBL" id="ODQ59581.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="953" mass="106629" checksum="78158C8C5BFE69E8" modified="2017-01-18" version="1">MSSNHKIRYDYDQDGNRRSKYLQTLENLDVPASGPAPQKHIHLITLRQYLKQRIIESLSPKKQLKQEPKGPFGTHKSMTSIKKPSYKDVPSKKLDFGTVPKDLNSKQASNNLASELLSAEDKIQPLSQSSISVPKIDNSFLVKRKENENHQQQHSTLSPTPHQPIMPKKSTLFVASDDSETETESENAISQLTLKRIFVSLDSDDEDAFDEDGHPHFLPLDQEKFQPVDITSPVKISSQRRTSQIGADINEQLNDELHSPAHISDHGSPEFHESTTDVFAIPEDTAVNLSPPSRRKLTIRKPIIAQPAKPQKPKLEPISKPSQPKKEQPEPEEEDDDEEDEKDDEVEEDEEVENSEDESDDTYESNSIESADDEQDGEEKDDADADPSFDEDSYSKKGRKKLEISDSELEGLFSSDDEEAKRRAIDRNKRKGLARRKTAAEIRRRKLKEQEELSTSNSSDDTRYTSAREISGSDNDDDSASESGRRPRLLRGKVHDDEYQEEVGEDEFVVDNSDDIIYSSDDEFEKLLRTIESEEKKTELRHRRRHRRRYSHISSESENSSEGEVPVRSRKKRKKSVISSSSVSGSSQKIDPPSSKASQETKLTQSQSQNPPVPVAKSQGASSSTGSQEKSALKDHQAKKVKSTTSNSKATLNGKQGSKKKQSETSKPQSTGSKNDAKSNTLKDMPILVPRGPEPQKAQRNTKHRTTIGGRTKTSNSSNVARSSDQGRTGIRIIRDVPKKRKVERPKKSNKTQTTQTTQTQTSPKASRNLVFPNRPEAKAAEHVDMSEEEELDEDDEEFKALYRKKVDAMKAIPSASESESEEAGKNDLSARDTDGGDLDSLFNSPTEESPSMNLSEDNGEEKQREAEKEIEEEIEKEQEKETAPEPTLPPAPIEPISVSATPTPALAPAETTTEPTYTVLSDSDDSDNESEDDDDLVLIDEDTFKKSFTPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2Q3</accession>
    <name>A0A1E3P2Q3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alcohol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_62195</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038815.1">
        <property type="nucleotide sequence ID" value="XM_019183324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2Q3"/>
    <dbReference type="STRING" id="683960.A0A1E3P2Q3"/>
    <dbReference type="GeneID" id="30200570"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000947">
        <property type="term" value="P:amino acid catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08297">
        <property type="entry name" value="CAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000002">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="10">
        <location>
            <begin position="18"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="350" mass="36960" checksum="36FFEC20FFE566C4" modified="2017-01-18" version="1">MSFEIPKTQKGVIFYENGGELHYKDIPVPTPKANEILINVKYSGVCHTDLHAWKGDWPLGVKLPLVGGHEGAGVVVAKGANVTNFEIGDYAGIKWLNGSCQSCEFCEKSYEANCPKADLSGYTHDGSFQQYATADAVQAAKISKGTDLTEVAPILCAGVTVYKALKTADLAAGEFVAISGAGGGLGTLAIQYAKALGLRVVAIDGGDEKGELVKSLGAEIYIDFTKTKDIVKDIIEATNGGPHGVINVSVSEKAIEQSTEYVRPCGTVVLVGLPAGAVAKAQVFGAVVKSVSIRGSYVGNRADTREAIDFFERGLVKAPIKVVGLSELPNVYKLMEEGKILGRYVVDTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2Y8</accession>
    <name>A0A1E3P2Y8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">F-actin-capping protein subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_69611</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
            <location evidence="1 9">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the F-actin-capping protein beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038454.1">
        <property type="nucleotide sequence ID" value="XM_019184175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2Y8"/>
    <dbReference type="STRING" id="683960.A0A1E3P2Y8"/>
    <dbReference type="GeneID" id="30201421"/>
    <dbReference type="OrthoDB" id="9979678at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099079">
        <property type="term" value="C:actin body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000142">
        <property type="term" value="C:cellular bud neck contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008290">
        <property type="term" value="C:F-actin capping protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904600">
        <property type="term" value="P:mating projection actin fusion focus assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902404">
        <property type="term" value="P:mitotic actomyosin contractile ring contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.570:FF:000001">
        <property type="entry name" value="F-actin-capping protein subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.210">
        <property type="entry name" value="F-actin capping protein, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037282">
        <property type="entry name" value="CapZ_alpha/beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042276">
        <property type="entry name" value="CapZ_alpha/beta_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001698">
        <property type="entry name" value="CAPZB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043175">
        <property type="entry name" value="CAPZB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019771">
        <property type="entry name" value="F-actin_capping_bsu_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10619">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10619:SF0">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT BETA ISOFORMS 1 AND 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01115">
        <property type="entry name" value="F_actin_cap_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00192">
        <property type="entry name" value="FACTINCAPB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90096">
        <property type="entry name" value="Subunits of heterodimeric actin filament capping protein Capz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00231">
        <property type="entry name" value="F_ACTIN_CAPPING_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0117">Actin capping</keyword>
    <keyword evidence="6 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="285" mass="32494" checksum="17A15EE14FB5C352" modified="2017-01-18" version="1">MSKKLNRKMSEESYDAALDLLRRLDPKNIAKNLQSIIKLNPDLAEDLLSSVDIPLNVARDEANGKKYLTCDYNRDLDSYRSPWSNKYYPVLEEGDDESPFPSEKLRQLEIYANDSFDVYRDLYYEGGVSSVYFWDQPDEEDSQNDSNFAGVVLLKKNTDDEQGLWDSIHVLEVLVGRSKATYKVTSTIILDLSKVDKDKKDSISLSGNLIRQTEKELAITDEHSHIINIGTLVEDIESKLRNLLQEVYFGKTRDIVGDIRSLQGLSSSEDDKKKQQELVKGIQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3F1</accession>
    <name>A0A1E3P3F1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sm protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_78470</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the snRNP SmB/SmN family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039045.1">
        <property type="nucleotide sequence ID" value="XM_019184726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3F1"/>
    <dbReference type="STRING" id="683960.A0A1E3P3F1"/>
    <dbReference type="GeneID" id="30201972"/>
    <dbReference type="OrthoDB" id="2020720at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070990">
        <property type="term" value="F:snRNP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036261">
        <property type="term" value="P:7-methylguanosine cap hypermethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01717">
        <property type="entry name" value="Sm_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050914">
        <property type="entry name" value="snRNP_SmB/NAA38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B AND N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Sm" evidence="12">
        <location>
            <begin position="7"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="105"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="161" mass="17837" checksum="01C474B2706D6D54" modified="2017-01-18" version="1">MVGVNVSKKTRLSDLINYRVKVITLDSRQFIGELLSFDKHYNLVLANTEEFRLTKKSLLSLKDRARNKSDEPESNLIQEQKRILGLVILRGEHVISVTIEAPPANQKSRLSVKQGSGVIKPLKNNNTKSTGQSLTAPVRTQSTPGFSGAPKGFNPPPGFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3Q1</accession>
    <name>A0A1E3P3Q1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial</fullName>
            <ecNumber evidence="6">1.1.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Isocitric dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">NAD(+)-specific ICDH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_92286</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-threo-isocitrate + NAD(+) = 2-oxoglutarate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:23632"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Octamer of two non-identical subunits IDH1 and IDH2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.41" evidence="6"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039316.1">
        <property type="nucleotide sequence ID" value="XM_019186353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3Q1"/>
    <dbReference type="STRING" id="683960.A0A1E3P3Q1"/>
    <dbReference type="GeneID" id="30203599"/>
    <dbReference type="OrthoDB" id="10261637at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045242">
        <property type="term" value="C:isocitrate dehydrogenase complex (NAD+)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000003">
        <property type="entry name" value="Isocitrate dehydrogenase [NAD] subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004434">
        <property type="entry name" value="Isocitrate_DH_NAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00175">
        <property type="entry name" value="mito_nad_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF34">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="15">
        <location>
            <begin position="38"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ60109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="369" mass="40020" checksum="B1B225B58B187C58" modified="2017-01-18" version="1">MLSSRLFFRPSTQHIARRTYATQVAKFNGKKNANGKYTVSFIEGDGIGIEISKAVKEIYAAANIPIEWESCDVTPLLVNGQTTIPQPAVDSIYKNLVALKGPLATPVGKGHKSLNLTLRKTFGLFANVRPAKSIAGYPTPYENVDTVLIRENTEGEYSGIEHTVVPGVVQSIKLITKEASERCIKYAFEYARSIGKPRVLVVHKATIMKLSDGLFVETAKEVAKQFPDVEIDFEIIDNTSLKLVTDPSKYSNVVMVMPNLYGDIMSDLASGLIGGLGLTPSGNMGDKVSIFEAVHGSAPDIAGQNKANPTALLLSSCMMLQHMGLESYGEKIHKAVLKTIASGKENRTGDLKGSATTSHFTEQIIKNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4K0</accession>
    <name>A0A1E3P4K0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA polymerase beta thumb domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_92816</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039608.1">
        <property type="nucleotide sequence ID" value="XM_019186379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4K0"/>
    <dbReference type="STRING" id="683960.A0A1E3P4K0"/>
    <dbReference type="GeneID" id="30203625"/>
    <dbReference type="OrthoDB" id="205514at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.210.10">
        <property type="entry name" value="DNA polymerase, thumb domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037160">
        <property type="entry name" value="DNA_Pol_thumb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022312">
        <property type="entry name" value="DNA_pol_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029398">
        <property type="entry name" value="PolB_thumb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11276:SF28">
        <property type="entry name" value="DNA POLYMERASE LAMBDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11276">
        <property type="entry name" value="DNA POLYMERASE TYPE-X FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14791">
        <property type="entry name" value="DNA_pol_B_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00869">
        <property type="entry name" value="DNAPOLX"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA polymerase beta thumb" evidence="4">
        <location>
            <begin position="71"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="143"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="174" mass="19966" checksum="E6A5C6B256404D99" modified="2017-01-18" version="1">MNEMKTILSKLRELLMKSDYLKCELNKPSSKLMAGCALGPVGNKKLTGKFTTGKCRRIDFLLVPWSEIGAALIYFTGNDNFNRALRFRASKMNLILNESGLFKRITYKQGQKVKDREMLIESFSERKIFKLLGVEYEAPEQRNMGSNEKLVDVKPDEIDENEVIKKNDEETDST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4K5</accession>
    <name>A0A1E3P4K5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_20837</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039417.1">
        <property type="nucleotide sequence ID" value="XM_019180887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4K5"/>
    <dbReference type="STRING" id="683960.A0A1E3P4K5"/>
    <dbReference type="GeneID" id="30198133"/>
    <dbReference type="OrthoDB" id="2261376at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001406">
        <property type="term" value="F:glycerophosphodiester transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000140">
        <property type="entry name" value="Putative MFS phospholipid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="346"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="374"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="412"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="57"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="494" mass="54786" checksum="65D7B39565777630" modified="2017-01-18" version="1" fragment="single">TRDIPKTPKQFLVSWVPRAKKEITLTKDQQNLAETEDEYNLRLQQVNEMGRFKKYLPIITTGAGLFSDGYVNNSIGTASTCLKLLYPKEYGSSPAIRNVTSIAFVGTVVGMLIFGVISDYHSRKFAMLAGTIILIVFTILCSGAWGAHDSIHGMLAALTAYRFFLGVGIGAEYPAGSTACAEASALLPPGKRNRWFCWFTNFMIDTGFVVSSFVPMVMLWICTPKHLTPVWRVTIGVGAIPPISLFILRLFYKEGEVFQKTRFKKVRVPYWSILKFYWFRLTIVSVIWFIYDFSVYAFGLFSSYVIAGLYDSDDLYKVFGWNVVLNLFYIPGAFLGAIVSDYLGPRLTLVIGVSLQSIIGYIMAGCFESLQKHVAAFVVVYGIFMTFGEFGPGDNIGLLASKTSCTPVRGQYYGLAAAFAKIGAFIGTYCFTPLTDRYGIKSAWWLASSLGLFAAFLALFLLPSVDQSAMQAEDAKFLEYLQSTGFDISQLGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4X2</accession>
    <name>A0A1E3P4X2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_29958</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019181671.1"/>
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    <dbReference type="STRING" id="683960.A0A1E3P4X2"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF60">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="146"/>
        </location>
    </feature>
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        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
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        <location>
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            <end position="285"/>
        </location>
    </feature>
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        <location>
            <begin position="291"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="ODQ60274.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="365" mass="40819" checksum="0DB16E1F21B6D117" modified="2017-01-18" version="1">MEKGNKDRQKGLHLFISSLGPTLGPCLSALIAGYIVERADWRWCFYVLCMFNFAIAVGGNLFLKETYAPTLLSRKAAKLRKLTGNQNLHCIYEVATGETTFGKFYVNLSRPLSVLFKNPMVLGLGIFMAVAYGCLYILIVTFPAVWGEVYGFGKGSTGLMYLSFLIGYIIGIVFFQYSATWVLHRLIAKNNGVSKPEFRLPMLLVSGITLPVGLFWYGWGAEKKLHFMFPAVGAAIFGFGLIAVFYCIQNYLIDMNPRFAASSIAAASIFRSYLGFAFPLFAPIMFKNVGYGWGCSIFGFIALATGIPFPLFVILYGERLRLWGNRIMDRAQAKRDAKNLARLKRMQEKQLVQSEQHKDATSESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5B6</accession>
    <name>A0A1E3P5B6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_62982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039631.1">
        <property type="nucleotide sequence ID" value="XM_019183471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5B6"/>
    <dbReference type="STRING" id="683960.A0A1E3P5B6"/>
    <dbReference type="GeneID" id="30200717"/>
    <dbReference type="OrthoDB" id="429143at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF279">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="1">
        <location>
            <begin position="40"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="468" mass="52352" checksum="4927EC7DFFC09095" modified="2017-01-18" version="1">MSKPNYLPVPNYTKPFWLSLQDTDPFSCYKSSERVPAKTDILIIGSGYAGTSTAYNLFNEDPSLDVTMVEARTICSGATGRNGGHLKPYCHRMYLDYEAAHGQFVAAQVVNFEVDHLYKIKQIVEKEEIDCDFVLTRACDVYRDSKRIENDLKAFDKMMANPYVRDDIKQSIQMVKGGDVATISKVPDAKLCFTYPAAHIWPWKLMTGLLKKCVAKGLKLYSNTPVTKVTKSDKTGEYIVETENGSIVAKKVVFATNGYTKGILNQFNEVIVPVKGLVTHIKPNDPSKPIPQLPNTYGLFDRVGLNSDYLINRSDGGVIVGGASNLMIKPNGDYSELFNVTDDSYYPERAAQHLQGYMQKFFSTWKDFKTVNDYTWSGILGFTNDKFPYVGELDFVGMRNAYIVAGFSGHGMPRVYLSGKAIADCITSGKSIQQVGVVPECFYASAKRLAKAEHDYQKEIENHIKSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5N7</accession>
    <name>A0A1E3P5N7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fumarylacetoacetase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_67433</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
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    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019184082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5N7"/>
    <dbReference type="STRING" id="683960.A0A1E3P5N7"/>
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    <dbReference type="OrthoDB" id="3978420at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050163">
        <property type="term" value="F:oxaloacetate tautomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="2-hydroxyhepta-2,4-diene-1,7-dioate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
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        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
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        <property type="entry name" value="ACYLPYRUVASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820:SF112">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_1G02370)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAH"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="3">
        <location>
            <begin position="72"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="293" mass="32425" checksum="A63FA32C705F0D60" modified="2017-01-18" version="1">MSFKRLIRFIGEDNQEYFGDAILPEDEIDVYKAKEATIIIGDVLKDYTLTNYTQKIKKLLSPLSPDQIPSLRCVGMNYTKHAIEIKMEIPKYPVLFFKPTSAIVGPSDPIRVPAACQIPPAKVDYEVEMAIVIGKEGKDIPLHKAHEYILGYTIGNDVSQRTWQMDRGGTQFGVGKMFDTFAPLGPCIVSSNVLPDTDGLNISCKVNGITRQDSNTSDMIFNCFEVVSFMSNGTTLKPGDVIFTGTPSGVAVGLNPSPWLKDGDEIVFEIDKIGTLKNKVVFENDPEITKLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5S2</accession>
    <name>A0A1E3P5S2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_105249</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039489.1">
        <property type="nucleotide sequence ID" value="XM_019180443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5S2"/>
    <dbReference type="STRING" id="683960.A0A1E3P5S2"/>
    <dbReference type="GeneID" id="30197689"/>
    <dbReference type="OrthoDB" id="269518at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070096">
        <property type="term" value="P:mitochondrial outer membrane translocase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045721">
        <property type="term" value="P:negative regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23797">
        <property type="entry name" value="UBCc_UBE2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000061">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="6">
        <location>
            <begin position="3"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="150"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="156"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="196" mass="22558" checksum="7042D5E00A57CBB4" modified="2017-01-18" version="1">MSSPKRRIETDVMKLLMSDYDVSLINDSMQEFYVRFKGPEDTPFQGGTWKIHVELPDQYPYKSPSIGFENKIFHPNIDEGSGSVCLDVINQTWSPMFDIFNIFEVFLPQLLRYPNPSDPLNGDASSLMLNDKKLYESKVREFVKRYASNPNLKVGEDDEESDEELSDVSSLSDNEDEDEIDIEDDDDDEMVGEMEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5V0</accession>
    <name>A0A1E3P5V0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Galactose oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_67474</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040012.1">
        <property type="nucleotide sequence ID" value="XM_019184085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5V0"/>
    <dbReference type="STRING" id="683960.A0A1E3P5V0"/>
    <dbReference type="GeneID" id="30201331"/>
    <dbReference type="OrthoDB" id="3980762at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133695" description="Galactose oxidase" evidence="3">
        <location>
            <begin position="23"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="410"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="465"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="531"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="349"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="471"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="483"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="498"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="559"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="700" mass="77800" checksum="C711B78C7BB378CD" modified="2017-01-18" version="1">MNLLGVEVWILLTLIISHVVKAQWDTAYSPTYSNAFLKLSNNEILQIPNNQSYNYGDIASNIITAPTKQNYSITVANGDLYAFYKGSIQSNYSISISIYNTNTSKWEGVKYNTTLSKNTNYQHFDDSTVMTSPDKDGHDSIYIYGGLYNNTVSNRILQYNPYTKTLNSIITSISPTGFYGSANAILDSKGTSNLLIGGKASSGWVSMFQIALWEYKSWTFKTVSSSSLSINSRINPLVLPVFKGDETHASSVLVLGGLLGTNLASPYVLNLNLTDDWKWQDFTKKTDFNVNDILGAVVLNSTLFTVNANSKKRSNGYTLHLYDTDGLKKVDTFDSAFKFSKTTTDDKSSSSSSSSSSESSVSSSISSSISSEASSEVTSSVSLENSSSSSILSFSSATGNVTPRSSNAKVIVPAVIVPIISIALISLLCFFIYKKYYKKESDLDDDTDSEHPDLYNEVNQIDNQSISSWNQKRDEYERRRYSQQRTPTRAKPTTTMISPSQQQRSPTRQSVDFSFDNTNNNLAKKIIKFSNHNKPSSQHQRSHGPFANPEDYADKQELSPMSNHANRSRVSLISSKGDTVSSSSEELAPSPFDEEEEQIDRFLGNRDVQVLVSSKRRSKLRITNPDVESMESISEAGSGSTIYDEKIGESNHDLNSRFTNNDVFHDENGIENDDYVREILKAFDDDNLSTDIGSFDKYDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5Z8</accession>
    <name>A0A1E3P5Z8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cargo-transport protein YPP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_77547</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in endocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic granule</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the YPP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040083.1">
        <property type="nucleotide sequence ID" value="XM_019184571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5Z8"/>
    <dbReference type="STRING" id="683960.A0A1E3P5Z8"/>
    <dbReference type="GeneID" id="30201817"/>
    <dbReference type="OrthoDB" id="29013at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23270">
        <property type="entry name" value="YPP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051722">
        <property type="entry name" value="Endocytosis_PI4K-reg_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083:SF464">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT DOMAIN 7, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN, TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="796" mass="92467" checksum="9F88E891229EA8A1" modified="2017-01-18" version="1">MGKVDNPIDTILQKKGFGIWPQDVVQVEQDDPLITALNIEYRFQQHLTINKLDGLIPLQNPDSKIDLFIEESKSLLALSSGLDPITKFILLINVGHFKFIQNQINDSIDILKAADLNFGSHANNYIQSLFYRKHFLLANSYHQLQDFTNEEKWLITGVNFFDKIPTLSNIESLKWLNLIYSKVLRLISTPTVSKLDKFFQNKNFIMSFIDYSIDQGIKYDPKELINFVTTRSNSLLSSTKFPKSDELNNYELEEFLSQVNDCKLIKPELSADLIEKGINKTYQSHILLRALTKNLLILGKKEESYHSFEVYVDYIERYYVQNNNSFNDILSILNTFKLMLSFRFRGNKSSDDLTLEEYDRYVSILNKFKKLLGNFYDDNGVLKIEQDFDLLDEDSLLLNVNNDKLRQFLSEIWYTLAISNIRLYKSSYSIFPKNESILKESIGFFKNSIYNDYKNDEIILQYVKFIATLRKIKESYSILKNFLTKQTIKNLTFFKSWHLLALIVSIEENKDESFKIINFLINEIDEFLELTQGESLSIELKNCFIQIKITHLSIIESLLGVEQSLDSLPELFALYNNLFKEPIVQESEKIESKSNQLHRTSTLAKIKSIRSHKEVKTTPAPAPPTIKISIEQAKLLQQIWLVTSTIYSKANLFEDAQQAVLEAEKSFKQTSETLTTLGLISIVDDPQLSLKSFEKALDIDRDYSLAIIGLSNLFLHQNSAQAKIFVNEKDSRAAIARVKILLELLTERFNSSMISEVWYLLSQIYEEYGDKKRFRDALWKSVDLEETRPIRDFRYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P605</accession>
    <name>A0A1E3P605_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ER membrane protein complex subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_28084</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040082.1">
        <property type="nucleotide sequence ID" value="XM_019181448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P605"/>
    <dbReference type="STRING" id="683960.A0A1E3P605"/>
    <dbReference type="GeneID" id="30198694"/>
    <dbReference type="OrthoDB" id="16510at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045050">
        <property type="term" value="P:protein insertion into ER membrane by stop-transfer membrane-anchor sequence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008504">
        <property type="entry name" value="Emc6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029008">
        <property type="entry name" value="EMC6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20994">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20994:SF0">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07019">
        <property type="entry name" value="EMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="86" mass="9928" checksum="0EF028C41ECB63C4" modified="2017-01-18" version="1" fragment="single">LQYIQDYTSLIFGTSAGILQLESYYGFAFFSITSLIITLLYVIFVTKLKPSLFYENPLNDIFLNNLSRSLTAYLMMWTLTYALVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P637</accession>
    <name>A0A1E3P637_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex component SEC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_61306</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SEC5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039947.1">
        <property type="nucleotide sequence ID" value="XM_019183154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P637"/>
    <dbReference type="STRING" id="683960.A0A1E3P637"/>
    <dbReference type="GeneID" id="30200400"/>
    <dbReference type="OrthoDB" id="26242at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048309">
        <property type="term" value="P:endoplasmic reticulum inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001927">
        <property type="term" value="P:exocyst assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029175">
        <property type="entry name" value="EXOC2/Sec5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039481">
        <property type="entry name" value="EXOC2/Sec5_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15469">
        <property type="entry name" value="Sec5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC2/Sec5 N-terminal" evidence="5">
        <location>
            <begin position="64"/>
            <end position="833"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF15469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="837" mass="96537" checksum="750D2978D41070D0" modified="2017-01-18" version="1">MEEEILKAYQLKTLNPQSWEEDSDEINPTNPITDKDFNDGDSYALLLSIVNKDSDLQEVHNALDPLNPHVTIQQVARERNLNGNDISKFSINSKQFNSKQFLKFIHKDDSFSQLSESLNILEGSIVERNKELRLLVEREFLRFVRSKSSLDGILSQFQKTGFNDDEVGLKSLKSSVNDANKEATLLIKPILQKKQREQKLKQALEYVEKKKLFFNLPKSFKNYINENDYDNLIHDYKNAKQLKEDNNDKIISRIWEELEMIIDTYKKTLWQSLTKDESDDDFLKSIKKLIELDVIDNPVLEWIDLKLKKFISLFNETFGKYHEKILNVQLNILSTVDEQDFTNFKSALESNRPLIDSALIIEMWLMIVKLIEKLQELVTNFIKFWNHVERFLNGTYQQRFVSNYIDPNSPYLKFEKYEIEDVKKKGETFIDLLVNKLIRFFSSTQDSLKTLHTSKTNENDGATDNFGFLPPFTNSLSALKYLPKIYTSTSEILNNLGQLAITDKTVQSLRDTSVLLSERIVGSVCASWLNDCRSFHNLENWENLNTGETLIPNLIYDYEVYVIKGLGKLLFSKLPEAKVAQIVKYPSKKILTGVQIQFLRSFDVLLESMIKKIIEENQNQTVSKDIKSHHKLLTLSNIKKLKTSVVPSTLEEFDNTFDSNLHSQNLEIYAILDKMELTIFDSYINEQRKHLSKILLTGISNTQWSSISSPPSKVSSFIYESIHLLIVVYTSVSKVSDNLIGKVLQDLLEYTSVNLLKDLREVGDFSIEGLRQVSLDVEFLKKLIGKSASQATVNNLNLVYKNVFGPRVDTKTILNSITPLLNDSLDGSRIEFNIFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6A7</accession>
    <name>A0A1E3P6A7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_28718</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040001.1">
        <property type="nucleotide sequence ID" value="XM_019181531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6A7"/>
    <dbReference type="STRING" id="683960.A0A1E3P6A7"/>
    <dbReference type="GeneID" id="30198777"/>
    <dbReference type="OrthoDB" id="277011at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000043">
        <property type="entry name" value="General stress response phosphoprotein phosphatase Psr1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="35"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="209" mass="24220" checksum="96FDA23E4666F306" modified="2017-01-18" version="1">MNEDETMDDFFDLQRLQQGQAHASNGWLLEDIEPKFKNKKCLVLDLDETLVHSSFKYLRQADFVIPVDIENQIHNVYVIKRPGVDEFLKRVGEIYEVVVFTASVSRYGDPLLDELDIHHAVHHRLFRDSCYNYQNNYIKNLSQLGRPLNDIIILDNSPASYIFHPQHAIPISSWFSDAHDNELLDIIPFLEDLAKDNVDDVGLVLDINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6D7</accession>
    <name>A0A1E3P6D7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Vacuolar protein sorting-associated protein 35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_28344</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Plays a role in vesicular protein sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the VPS35 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040067.1">
        <property type="nucleotide sequence ID" value="XM_019181478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6D7"/>
    <dbReference type="STRING" id="683960.A0A1E3P6D7"/>
    <dbReference type="GeneID" id="30198724"/>
    <dbReference type="OrthoDB" id="10258141at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170071">
        <property type="term" value="C:CROP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030906">
        <property type="term" value="C:retromer, cargo-selective complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140312">
        <property type="term" value="F:cargo adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900102">
        <property type="term" value="P:negative regulation of endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.660:FF:000003">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.660">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35, helical subcomplex Vps35-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005378">
        <property type="entry name" value="Vps35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042491">
        <property type="entry name" value="Vps35_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03635">
        <property type="entry name" value="Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009375">
        <property type="entry name" value="Retromer_Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="767" mass="88861" checksum="50C2EEC3C2100548" modified="2017-01-18" version="1">MSEPITPEEQNALLQDSINVIKQETAQMKRSLETRGKLMDALKHASLFLTELRTSQLTPKQYYELYMMVFDSLNLLANFLKDNHPHHHLADLYELVQYAGNIVPRLYLMITVGTVYMSVPDAPVKEIMKDMIEMCRGVQYPIRGLFLRYYLSQRAKSLLPENQDSIQFVITNFIEMNKLWVRLQHQGPSKERLKRVKEREELKILVGSNLVRLSQLQNDKEDYKTSVLPAILEQIVQCKDVLAQEYLLDVVIQVFPDEFHLYTLDEYLNCIKDLNPNVSIKKVLITLIDRLTDFAKQTSEVPKEEKKINLFETFWYYVNQLEQSRELSIEEISGLLESVSRLTLTYYPTNYGNIDLIFKFAIDKIKELSTSSSQELETDQNFKSLLLAPINNYDNLLDILKLKNFGDLLDSEPIKIQKIVSLEILNKILNAGIRLKKIEHVDGSFKLLRVLIENKEKTTEESVEFITEQEKLAKIIHLIGNKDTTIHAKLLANAKGALSKGNIKHTYPALFFNTLKLIRKNKLKENQNSLFKFISRVINDLYRIGLNDQSFKLYLASASIADQVQAEEIAYEFFAQAFTIYEEAISDSRLQFQSIVSIAGVLQQCRNFSRENYDTLITKTALHGSKLLKKPDQCRSVYLSSHLWWGVEIPALGEEEGVSEFYRDGKRVLECLQRSLRVADACMDSSVSIELFVEILNRCLYYFIHGNESITIKYINGLIELIQTNLESSVTNENGQINENPKKHFQRTLEYIESQKEIDERFQLITW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6G0</accession>
    <name>A0A1E3P6G0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_61453</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040097.1">
        <property type="nucleotide sequence ID" value="XM_019183179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6G0"/>
    <dbReference type="GeneID" id="30200425"/>
    <dbReference type="OrthoDB" id="5229455at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF46">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00755">
        <property type="entry name" value="AFLATOXINBRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="33"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="74"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="598" mass="69169" checksum="CDF9CCDA86FF91A5" modified="2017-01-18" version="1">MILNELNSGKTPNNNNNDNNNSNQQKLKRSRDGCQNCKEGRIKCDKTKPICKVCQRKGLNCDYSKKLMWISVDKTDSRHPSFKNKKPPQKTTKPPTKVKKDTHLEDKIIPKIDAIEERDMLDTTEEYELLTPSRRNSEVRSRNSSIISNGDDNNNGEFIDEEYIDNLSDDFSESVQLDLDTISHHGLMKLDFQKIVESQRNFSNNFHSFPREISNIFQSQDTQNIFSHYLFVTCISLDPLNGAGPQNISEKFFVPVAVNDPATLHGILSISASQLSFSNPEYKRLSLLHKSLSYKHLYALLKDDKQSISLNALSAIICHLSIEIIQSGLQNWPVHIKALRKIVQERKKRNMKEDQLTWFLYLRIARYDNFATLTTGEKPLFKEFNAVDFKSFHPLIQNFDCIFGCPTTVLFFCSELTYIGKTIFDSKQDGKFKSLGDHLETLNYSEIGLKKRLTKLLKRNQSSINANDWNAIEKIWSSALIIHFQQAVYDSSSQQYDFEIEKENFFDALKEFVLYDSFFCSNLLWPLLKISTILIDPNDRDFLRKTIGAFFDKTNIGAFKGVLKIVDELWELIDEGTFTKPSDWWVFHEKKGWNIFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6G2</accession>
    <name>A0A1E3P6G2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribulokinase/uridine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_79043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039654.1">
        <property type="nucleotide sequence ID" value="XM_019184830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6G2"/>
    <dbReference type="STRING" id="683960.A0A1E3P6G2"/>
    <dbReference type="GeneID" id="30202076"/>
    <dbReference type="OrthoDB" id="6362633at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="1">
        <location>
            <begin position="26"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="224" mass="25555" checksum="0D7F5DFCE64F9C8B" modified="2017-01-18" version="1">MDQTENNLALRALKLLEQIPSDQRLLIGLAGSPGSGKSTISKHVVQKINELYGSEIAIVVTQDGFHYYRSELEKFPNREEAFKRRGAPFTFNSKKFLKLVKLLREPINENVVITAPDFDHKLKDPKPRAIKVEPNHMIVIIEGNYVLLKDENWKEIGSLVDERWKINVDSTTARSRIVKRHLESGISNSMEEAIKRCDGNDMINAEYIRLNSCVPDLVIQSIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6J2</accession>
    <name>A0A1E3P6J2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">D-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="9">1.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">D-lactate ferricytochrome C oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_28347</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(R)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + pyruvate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13521"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.1.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.4" evidence="9"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040160.1">
        <property type="nucleotide sequence ID" value="XM_019181479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6J2"/>
    <dbReference type="STRING" id="683960.A0A1E3P6J2"/>
    <dbReference type="GeneID" id="30198725"/>
    <dbReference type="OrthoDB" id="7786253at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000014">
        <property type="entry name" value="D-lactate dehydrogenase (Cytochrome), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF111">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="13">
        <location>
            <begin position="137"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ60953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="570" mass="63421" checksum="D7EFAE8BB0935759" modified="2017-01-18" version="1">MFYRGAAPKIGFRITQRQRFQRTNQFQRFLASSAKSTKTDAKSPSRVLLISVFTTGAIVGFYFAVRKVLNNPPEFLFPSSSKTELADLPSPQYANLLEFKVAKDKFLELLGKENVSETSNDIETHTKNNYTLHEPEPNEKPQLILYPSSTAQVSEIMKIAHDFKIPIVPFSGGTSIEGQYISTRQPCIIVDLSKLNKIIKFNEKDLDITVGAGVSWLDINEYVEPFGLMFGPDPAPGALIGGMIGTSCSGTNAMRYGTMKENVVNLTVVLADGSVIKTKQRAKKSSNGYNLTNLFIGSEGTLGIVTEATVKLHIKPKYETVAVVSFNSIVDATDTVTDLISQGVQLNAVELLDEKMMSCINYSNQTSKKWDVKPTLFFKIGNNNSKILKELIKDVQNISKTHNYHNFQFAKNEAQQVELWSARKSIFWSSIDYGRSQVSPDVKIWSTDVAVPISNLSSMIEETIKDTEQTGLYTTIVGHVGDGNFHCLVMYDVKDSAKAMALVNRMTERSIKYEGTCSGEHGIGVSKRSHLEVELGKDTIDVMRKIKLSLDPLKIMNPDKIFKIDPSDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6K3</accession>
    <name>A0A1E3P6K3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Succinyl-CoA:3-ketoacid-coenzyme A transferase</fullName>
            <ecNumber evidence="3">2.8.3.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_83305</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 3-oxo acid + succinyl-CoA = a 3-oxoacyl-CoA + succinate</text>
            <dbReference type="Rhea" id="RHEA:24564"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:35973"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="2.8.3.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Ketone metabolism; succinyl-CoA degradation; acetoacetyl-CoA from succinyl-CoA: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 3-oxoacid CoA-transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.3.5" evidence="3"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040272.1">
        <property type="nucleotide sequence ID" value="XM_019185521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6K3"/>
    <dbReference type="STRING" id="683960.A0A1E3P6K3"/>
    <dbReference type="GeneID" id="30202767"/>
    <dbReference type="OrthoDB" id="1933379at2759"/>
    <dbReference type="UniPathway" id="UPA00929">
        <property type="reaction ID" value="UER00894"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008260">
        <property type="term" value="F:succinyl-CoA:3-oxo-acid CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046952">
        <property type="term" value="P:ketone body catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.10">
        <property type="entry name" value="Glutaconate Coenzyme A-transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012792">
        <property type="entry name" value="3-oxoacid_CoA-transf_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012791">
        <property type="entry name" value="3-oxoacid_CoA-transf_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014388">
        <property type="entry name" value="3-oxoacid_CoA-transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004165">
        <property type="entry name" value="CoA_trans_fam_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004164">
        <property type="entry name" value="CoA_transf_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02429">
        <property type="entry name" value="pcaI_scoA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02428">
        <property type="entry name" value="pcaJ_scoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707">
        <property type="entry name" value="KETOACID-COENZYME A TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707:SF23">
        <property type="entry name" value="SUCCINYL-COA:3-KETOACID-COENZYME A TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000858">
        <property type="entry name" value="SCOT-t"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01274">
        <property type="entry name" value="COA_TRANSF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="5-glutamyl coenzyme A thioester intermediate" evidence="4">
        <location>
            <position position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000858-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="500" mass="54174" checksum="322EA634028BD610" modified="2017-01-18" version="1">MSFGDFVSSLAVDILAIGSRISKVVQTTDEALEGIQSGQTLLSGGFGLSGVSDTLINALLEKKEINNLTAVSNNAGLDGQGLSKLLQSGQITKMIASFIGMNKTFEKLYLDGKIDLELTPQGTMVEKVRAGGAGIPAFYTPTGVGTWVEEGKLPVRYGENGEVLKTTKPKETREFNGRKFLLEDSIFADVAFVKAFKADTLGNCWFRGSSQNFNTAFGKNAKLTIVEAENIVEPGEIKPENVHLQSVYVDRIIKSTTPKEIEIVKLAEDQKSPEELLSSGNEKELKRFKISQRASKEFNGFKSANLGIGMPNLSVAFLPEDSQVMLQSENGILGMGPHPRKGEEDADLINSGKETVTLLKGASLFGSEESFAMIRGGKVGLTMLGGLQVSENGDLANWGLPGRVKGMGGAMDLVSNPQATRVVVLMEHVDKKGRSKILKKCQFPLTGTKCVKRIITDLAVFDVVDNDHLKLIEIADNTTLEEVKEKTDADFEIADDLKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6N0</accession>
    <name>A0A1E3P6N0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_28229</name>
        <name evidence="6" type="ORF">WICANDRAFT_28941</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
            <strain evidence="6">NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040215.1">
        <property type="nucleotide sequence ID" value="XM_019181558.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040307.1">
        <property type="nucleotide sequence ID" value="XM_019181462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6N0"/>
    <dbReference type="STRING" id="683960.A0A1E3P6N0"/>
    <dbReference type="GeneID" id="30198708"/>
    <dbReference type="GeneID" id="30198804"/>
    <dbReference type="OrthoDB" id="5511599at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039297">
        <property type="entry name" value="COX7a"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02238">
        <property type="entry name" value="COX7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="58" mass="6888" checksum="1CE43225B5DE07CA" modified="2017-01-18" version="1" fragment="single">NRVIELQKLYQSSPKPLWMKHPRSKFYIYPFWALFTGVTAINLYYTGRAILGIKDPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6R5</accession>
    <name>A0A1E3P6R5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Heat shock transcription factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Heat shock factor protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_23695</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">DNA-binding transcription factor that specifically binds heat shock promoter elements (HSE) and activates transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homotrimer. Homotrimerization increases the affinity of HSF1 to DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040250.1">
        <property type="nucleotide sequence ID" value="XM_019180999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6R5"/>
    <dbReference type="STRING" id="683960.A0A1E3P6R5"/>
    <dbReference type="GeneID" id="30198245"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032993">
        <property type="term" value="C:protein-DNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000027">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF427">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="13">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="99"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00059868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00084017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ61043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="195" mass="22547" checksum="8223A1B620139959" modified="2017-01-18" version="1" fragment="single">PAFVLKLWTMVNDPNNHDLINWYHDGNSFLVTNRELFVQKILPKYFKHSNFASFVRQLNMYGWHKVQDASSGSLHSDEKWQFINPNFQKGRPDLLDKIVRNKPNDEHNNSTQGSGGGNNQSNDGNGTNGQFDVSLLINELNTLKANQMKITQELSRVRSDNESLWQELFQTREKNLVQNEKIEKILHFLASVYGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7B4</accession>
    <name>A0A1E3P7B4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_77761</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040321.1">
        <property type="nucleotide sequence ID" value="XM_019184609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7B4"/>
    <dbReference type="GeneID" id="30201855"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="190" mass="21143" checksum="7FC8E73EEA162EF3" modified="2017-01-18" version="1">MLNSQHLDSTTNDTLEDAIPQNDRPPSCQVTINNVNEATTETADQPETTSITEISIKSSQTPLPDYNQAVQIQQNLPSPAATCSTRPPRYTLNGDPSYIDGIQRQTASGTNPYDNVFSIENVFIQREQALQQRRSASNNNDNRIYLNNAQGDNPIILNCTIPCWQFIVVVLLIYAVILLFFAAITRNNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7F7</accession>
    <name>A0A1E3P7F7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_29394</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040417.1">
        <property type="nucleotide sequence ID" value="XM_019181600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7F7"/>
    <dbReference type="STRING" id="683960.A0A1E3P7F7"/>
    <dbReference type="GeneID" id="30198846"/>
    <dbReference type="OrthoDB" id="10262413at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051783">
        <property type="entry name" value="NAD(P)-dependent_oxidoreduct"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48079:SF6">
        <property type="entry name" value="NAD(P)-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48079">
        <property type="entry name" value="PROTEIN YEEZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="14"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="338" mass="38069" checksum="9924F482243438F4" modified="2017-01-18" version="1">MTVEQPTTYQRQKVLLLGATGFIGGEILYKLLTLPQFEKLDITILTRCYEKARKIHSTTQGRVRPIVGSVRDVFLMHHEFQRADIVINANGVDSIRCAKYLSKVASKVRKPYLILHTSGASTISATKGASDRVFSDLENNNEIVHTPVDEIILGIEERNPKFVKTVIVSPPTIFGRNDGFIRREAVSIPKLVKLAMKNKQAFTAYSGDYHLSHVHINDLADLYVMLLEHLLVGNNIKTGREGYYFAENGVHDWKDISAQIGKLLKQKGLVKTEEVAQLQPEQIQKLANDETAPYYWGTNAVTKADLARSYGWQPKFTTADLLNDLETTVDHIALQENY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7K4</accession>
    <name>A0A1E3P7K4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DWNN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_13338</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040616.1">
        <property type="nucleotide sequence ID" value="XM_019180569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7K4"/>
    <dbReference type="STRING" id="683960.A0A1E3P7K4"/>
    <dbReference type="GeneID" id="30197815"/>
    <dbReference type="OrthoDB" id="106784at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000005">
        <property type="entry name" value="E3 ubiquitin-protein ligase RBBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000070">
        <property type="entry name" value="E3 ubiquitin-protein ligase RBBP6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014891">
        <property type="entry name" value="DWNN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033489">
        <property type="entry name" value="RBBP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025829">
        <property type="entry name" value="Zn_knuckle_CX2CX3GHX4C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439:SF0">
        <property type="entry name" value="CELL DIVISION CYCLE AND APOPTOSIS REGULATOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08783">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13696">
        <property type="entry name" value="zf-CCHC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01180">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51282">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DWNN" evidence="9">
        <location>
            <begin position="5"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="8">
        <location>
            <begin position="174"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="77"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="344"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="344"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="370"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ61409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="395" mass="44955" checksum="24D1512619A4C2AB" modified="2017-01-18" version="1" fragment="single">MSSTIFYRFRSQKNSSRIQFDGTGLTVYDLKKEIINENKLGTGLDFDLRLYNNDTNEEYQDDAVVIPRSTSVIARRSPATRNGKGTAARYVTGKPRATKIELKPQQQTQATQQDNNNQTQQTEEERINAMFANQSDQWKQTQQVMSTATPVYNKPAASSTTNNPDDVPPPGYMCYRCGSKDHWIKNCPTNDDPNWEGKRVKRTTGIPKTYLKTIEKPEDESTGVKSYMMNEEGKYVVQVADEKSWETYKKKVNKTQDMINDMKDIDPALIDPSTGKVFQDPVNTPCCDSIFSKQVIEDKLLESDFVCPKCGKEDIYLDQLKPNVELNKKVEEFLDSEFEKRGVKRTTQEDLEQSKRQNIGSLPIKPDASQQQQQQPGGQIPMMPMNPFGLPFVPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7M1</accession>
    <name>A0A1E3P7M1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RAM signaling network component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_83568</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019185563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7M1"/>
    <dbReference type="STRING" id="683960.A0A1E3P7M1"/>
    <dbReference type="GeneID" id="30202809"/>
    <dbReference type="OrthoDB" id="1394818at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019487">
        <property type="entry name" value="RAM_signalling_pathway_SOG2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10428">
        <property type="entry name" value="SOG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="218"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="503"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="298"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="503"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="513"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="782" mass="87739" checksum="976CA3C34CBEC2EF" modified="2017-01-18" version="1">MSSEQVQTSRTRLMQLITEELTRSIKSEDDVGGMKLIGLNIQSIPPEAVELIRESVERLSLQNNNISNLPQYFNRLSKLRYLDIHDNQFSTFPDILTTMSELEILDISFNNLRSLPNDLGHLKNLKVLSLKNNNFEILPTSILSIKNLKILEIENNPLKLPSQAFINNIPHKIHSQEWLQEIKNYLVYNKDLIQSNLSEIEHLQNGNVNMATTPIKETEKPPLVSSQSTISSNTSHPSSSVQHLERSRSTSESYVSSRAAKRMGFIVKRPNPEPTPEAVNEVESPPVEPLSITRRNRSMSAASSVSSHSPIPSTIDEDQETIGKDTKLQIPSGGFSPMNTDKLDSIPEHKSNDYFKRLSTLPEQRPNLTQFKIVDISRKILFSITEFQSTIKKLNAFCSEKSIIVDMVSQLYYSKNVIDHLVESLEKTEQNDDGYVAIDEILKSVTQTVSIFKKMIDILQSNYQAYVKNADVCFIRMLLLSLFLSYGEVYNAFRILNPESQSTQRQNKDIYSNNNHNNNNNNSRNAPPQLPPLKVNDLGMNPTITQQQLNEYGEADQKLYESLHNALESSSQVYAELNDAISKSAIANAVKPTEEQQQNLNGNTISKIKELTNTCVVSMDLNRKIKTKLGSNGFKTINVVEKKKFFDEINALLKSIITILGSTKSIMNEMPILNEVRPSLASLTKATKDVTIMLEVSSYRLINDSFSSLNSAVPQSAASMQAPMLSSIPSVMSIPQMNTPFLGMPSVHTPLQSAAVTHPNSNPFEATLANNLQHEENLDTIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7P7</accession>
    <name>A0A1E3P7P7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Topoisomerase 1-associated factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_28594</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the timeless family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019181511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7P7"/>
    <dbReference type="STRING" id="683960.A0A1E3P7P7"/>
    <dbReference type="GeneID" id="30198757"/>
    <dbReference type="OrthoDB" id="310853at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031298">
        <property type="term" value="C:replication fork protection complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044998">
        <property type="entry name" value="Timeless"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006906">
        <property type="entry name" value="Timeless_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940:SF4">
        <property type="entry name" value="PROTEIN TIMELESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940">
        <property type="entry name" value="TIMEOUT/TIMELESS-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04821">
        <property type="entry name" value="TIMELESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0236">DNA replication inhibitor</keyword>
    <keyword evidence="8" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Timeless N-terminal" evidence="11">
        <location>
            <begin position="56"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="617"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="993"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1130"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="624"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1001"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1031"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1141"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1211"/>
            <end position="1220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ61388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1264" mass="143192" checksum="716B3C6632E836C0" modified="2017-01-18" version="1">MSDVEDEAQVPTYSNEGIGPTLTKQEEERRLIRSYIAVLTSALGGVDYSSEETNPPYRLGDEAFACLKDIKKWLKSYDEERNTWEVAVACAESGLVVNDLIVILCDWEAGELNKNGPEYKKKMNDKIALACLELLVPLTWPLQLSEGMYKGQLQHYSNLKKHQVNYKKNILSYRGGKVLKAVVRLALPIMATPRSERSGRDNGILRLCTFFFRNVLAIEPASITKETSKHLKPSDIADNMPSGVNAEDISLGACLVAFEENLVFQYLLTICGSINKDFDGQFLSMACLEMIYDLTRGVRASVVIQERRKQVPLGATPPPSASTISTNQSTTSLGLSDLLSKESEMKTKFKSVGSSRHSRFGTLLSLQTPDQGRLTLSGQQNLSERQNTIAAFDSHKKWHKAQPFRYDSDDNVVFTEVHLSHQPNKILNKFTNDFLDGGFNPLINAISKDFTSESEDRPNTSKIHFLLVIAWFLEAERHRNGAKANNIDYGLIVAGLTEKTFILVFKYLTEGFDNKVWSLVHAAVITFKEIVTTTNFMALSESEEDKEIATNIKERLFHKKHYLDLMAKVPTYAYKHSPTYVNNCVDLVDVVLKTLEEFANEDMVLFIQSNRKQRKRKNVSREQEEIDDMEGSDGEELERTSRKLSAQRKFDFKKYQTQFVNAAAIETYMAYLSRFEDLSEKDIKKGLKFLHRAFYIQKNHVVLFRLDFMLLLHRLLSGDGLPKRSNIRKHFSEFLSYYMKKLKKSLDTTPSLYIELLFDKFHDTRTEHYLGTGEVYVEPKKIKRVTKYALLKGDEDMDLETKVSILVAGLIDDDMKHLAEWVGEELEKALMSRIHNNIPREQEDDELPEQKPIVDFVLKSNDVKLMNSLIINPKLRYLISLAGCELPSQTGDNCILKADSDTTRLSETVTYIKMYLIQPVAFDNDKNANDFIKIKQIRGAFEGDSDDGYNGGGDDVYGWDDDDIAFEVKGEKVDEGYNDDILDNLEDAIGKTDRALPKGVARSKNKPKREGRRRKSENADSDSDDDDRPRQRARKKRRNRGPALPYHDLSNDEGEAGASKKKAQVKSHDYVSAKYVNDSADESEDEEFFANEQRLREILIKNNGVLGAEQLQEFVNRTMNGAASESEFANGNVYISEDEKEEPKAAERDASDSESDLENSINRSEVLDQGSDTGSLSDEQMGEPTQAKKATFDEGSDLEGPSIDFGYDLNNDSDDEDLMLIDEVAVNPPVPFNDEKIKDESDEGEPSVAPRRKTKVVFSDDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7U2</accession>
    <name>A0A1E3P7U2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_78112</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040693.1">
        <property type="nucleotide sequence ID" value="XM_019184664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7U2"/>
    <dbReference type="GeneID" id="30201910"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133720" description="Secreted protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="185" mass="19341" checksum="E02C597A31ACF194" modified="2017-01-18" version="1">MVLAQTILQSVLFALVANAAAIPIQDGQKFNSSAYSVPPLSVPSVSLDPGSASIASPSFSSSPSSVVAQSAASNPSSFSSVSVPIASPPPSDATVPRVSPQDKKIKQEQQYQKRDDDDDDDDDKDDDKDKDDDEDDDQSSSASPSEFIQFVSSSSSASVSATPTADDHDDDHDDDKDGDKDGDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P823</accession>
    <name>A0A1E3P823_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Splicing factor YJU2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_83640</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the spliceosome which catalyzes two sequential transesterification reactions, first the excision of the non-coding intron from pre-mRNA and then the ligation of the coding exons to form the mature mRNA. Plays a role in stabilizing the structure of the spliceosome catalytic core and docking of the branch helix into the active site, producing 5'-exon and lariat intron-3'-intermediates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the spliceosome. Present in the activated B complex, the catalytically activated B* complex which catalyzes the branching, the catalytic step 1 C complex catalyzing the exon ligation, and the postcatalytic P complex containing the ligated exons (mRNA) and the excised lariat intron.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CWC16 family. YJU2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040735.1">
        <property type="nucleotide sequence ID" value="XM_019185573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P823"/>
    <dbReference type="STRING" id="683960.A0A1E3P823"/>
    <dbReference type="GeneID" id="30202819"/>
    <dbReference type="OrthoDB" id="674963at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000384">
        <property type="term" value="F:first spliceosomal transesterification activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000349">
        <property type="term" value="P:generation of catalytic spliceosome for first transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03226">
        <property type="entry name" value="YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007590">
        <property type="entry name" value="Saf4/Yju2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043701">
        <property type="entry name" value="Yju2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111">
        <property type="entry name" value="SPLICING FACTOR YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111:SF1">
        <property type="entry name" value="SPLICING FACTOR YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04502">
        <property type="entry name" value="Saf4_Yju2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="180"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="228"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="267"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ61528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="298" mass="34263" checksum="5AC0C489EA1E6EFE" modified="2017-01-18" version="1">MSERKVINKYYPPDYDPSKIIKKKKKVKTGSASLQTVRLMTPFSMKCLNCGEYISKSKKFNARKENTSESYLGMKIVRFHIKCPRCASEILFRTDPKSADYVLEFGAQKNYDSGNTNKIMKEETQEETLERLEKEEEEAKRKELEKDKKQTSLEELEAKLNDIRHQQEISEQIDEIRERNAKISERATNAELQKEIKERMRKAATERLVQENEEDEKLAEAAFKKQQPESNATQDGSDGSAEVDNTYTNKEASSGSESDSDSDSYSQPLFSKKTSVPQKVVMKRKNNSLGISVKKLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P895</accession>
    <name>A0A1E3P895_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_75842</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ61636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019184270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P895"/>
    <dbReference type="GeneID" id="30201516"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="250" mass="29117" checksum="985252A7E61AC4FF" modified="2017-01-18" version="1">MFKKLFKRTSNNEFTVRMSEPKESYYLGDRNGLVFETQNIGELGLNLKEIMPRVIDDVDHREFNEIPMILSELIVTDYKGNSGTIILLKPADSDMVTELEYFGDCIRKDDESAFIDPNPSRSLFKKAPAIDNFRMRVTFINIPKSIKSIDLFEYLVKHPPLETKVVFVNVDITNYYDKGEKNEEQFVLNIIKSQKMDLKDQLKEFEKALAPDQILKYPKVFVKMIMTFHQNVELLPSYTETELPAYSSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8J8</accession>
    <name>A0A1E3P8J8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Autophagy-related protein 33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_86656</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ATG33 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040942.1">
        <property type="nucleotide sequence ID" value="XM_019186063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8J8"/>
    <dbReference type="STRING" id="683960.A0A1E3P8J8"/>
    <dbReference type="GeneID" id="30203309"/>
    <dbReference type="OrthoDB" id="5336366at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051668">
        <property type="entry name" value="ATG33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37278">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 33-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37278:SF1">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 33-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="23621" checksum="5EC1CDE6A68EA035" modified="2017-01-18" version="1">MGVCLAVTKVIGTTTLGIYTGIITSNITNYDILIHVLQSTSISNFNEFNSNLKSKLQKNSILLGLIGGVSSLAFQLSYFKAPLRSKHPYLIYASLVLPLSSIIYGILTRGELIKYFKLDEVINSFKQSEDGSNVKKVKENVRSELDNSVYKDLGDDSSSNEGGQQEDDEISKEVEIHLNKTSLEQLLGKLNLANKIVGGVSFIGFVIASVGIYGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8P6</accession>
    <name>A0A1E3P8P6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_59864</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ61786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040993.1">
        <property type="nucleotide sequence ID" value="XM_019182889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8P6"/>
    <dbReference type="STRING" id="683960.A0A1E3P8P6"/>
    <dbReference type="GeneID" id="30200135"/>
    <dbReference type="OrthoDB" id="3979850at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="51041" checksum="42EA53D7C0F30C6E" modified="2017-01-18" version="1">MSSNDQLSELKEVVKKYRGPILIERLYHFKSSDPQIVGQAAFEALGYLESVDQCSPYIDQFIERLKVLPSNEAISRFQDPQWNYQVQNNKSKSAKAVGEQDLYHETNVKKTIDIAEFHTFNKQYFEATNALSTSAIGSPTCQLAEYSAQVALIQGNYSAVLNQDKRVEAFLTTSLSNELYLKRLTDQEVTSYWRVKYLILMAHFLKQEYEEAANLIYDLVDAPAIQSASGKQISALQLLKDSDFGQYIYEDEIVLASFISILLYKDPSELNNTLNDTEFTTLTGSKITDIRPFFISLNAAKFKELNILLDKLNYVGETNVFFNRVWSQIRLKFRQKSYSLYLRLAVRVSADHLSNRLNIEKGKLVKEINDYIKEKRLEFSYDAENEIFETNKKDKRIDFAESLQKLCEASDTVENLLREKLENAQKNALKAKNALNKRQDEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8Y8</accession>
    <name>A0A1E3P8Y8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PRA1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_67996</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PRA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040619.1">
        <property type="nucleotide sequence ID" value="XM_019184108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8Y8"/>
    <dbReference type="STRING" id="683960.A0A1E3P8Y8"/>
    <dbReference type="GeneID" id="30201354"/>
    <dbReference type="OrthoDB" id="63113at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004895">
        <property type="entry name" value="Prenylated_rab_accept_PRA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317:SF0">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03208">
        <property type="entry name" value="PRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="126"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="179" mass="20404" checksum="07E45C7ED39033BC" modified="2017-01-18" version="1">MSAFPSQFNAAQFKFNKWDLDTIKHQNPYLHHKITNIKPLKEFFNLSKLNTPKTINEAKTRLLFNLNFFNSNYLIIVLIISIFLLFQKPMLLISLIGLIGGFIVLNNYEFEQIRIGNVNIHLNKTYLYLGLIILTTPVIFYSSPISTLISLSSISGGIIITHGLLIDNLPETSFNESVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P962</accession>
    <name>A0A1E3P962_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_86980</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
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    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041152.1">
        <property type="nucleotide sequence ID" value="XM_019186086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P962"/>
    <dbReference type="STRING" id="683960.A0A1E3P962"/>
    <dbReference type="GeneID" id="30203332"/>
    <dbReference type="OrthoDB" id="427213at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018045">
        <property type="entry name" value="S04_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="389"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="424"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="486"/>
            <end position="513"/>
        </location>
    </feature>
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        <location>
            <begin position="547"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ61945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="686" mass="75298" checksum="6B19E537D1D4400C" modified="2017-01-18" version="1">MPSESSSLLNKQSGSSQHSNYESLPSPRRPRTRVSNLRYSESPSGAPRSFSPQRLHKPSSVNSEESVRPSSESEHLKWSQRVPYYLPCYSWLPSYNLNKFFGDLVAGLSLASFQIPLALSYATAIAHVPALCGLYSLAIPPIIYAVFGSVPQMIVGPEGAISLVVGQAVEPLMSHDKHVDPVDLVSVLSCIGGFYLLGAGLCRFGFLDNVLSRALLRGFISAVGIVMIINSLYDELGLNLIKDIPAHFHSPVRKFIFIIETINRSHRPTAIISFVSFVLIQVTQHIKGKLIKKGYNRWVFLPEILIVVVSSTILSAVYDWKSLGVAVVGKIKTKGFSYKFPISKETMPLYHSLMSSGFLAATLGFFESTTASKSLGSAYDLPISSNRELVALGSINLCGGIIGALPAFGGYGRSRINAISGAQTTMSGLIMGSVTLITIFFLLDYIYYLPQCVLSVITTIVGLKLLDETPADLRFHIRAKGYSELITFCITITATLLYSVEAGIALGVGYSVIRVIKNSTKSGIQILGRLPNTNTFLNADEPLPEDQGEFLEEIEGCMIVKIAEPLTFSNASDLKLRLMRLERYGSAKTHPAAPRTREDSMNKHILFDLNGMTYFDSSAAQIFYEILEAYKKKVPTKHGIRKRIKNSGISDLTDGKFYNSIDEVMMLLDNEEASQIHQVPDTASIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9E1</accession>
    <name>A0A1E3P9E1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Peroxin/Ferlin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_23028</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041236.1">
        <property type="nucleotide sequence ID" value="XM_019180973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9E1"/>
    <dbReference type="STRING" id="683960.A0A1E3P9E1"/>
    <dbReference type="GeneID" id="30198219"/>
    <dbReference type="OrthoDB" id="5586090at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006614">
        <property type="entry name" value="Peroxin/Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052646">
        <property type="entry name" value="Peroxisomal_PEX28-32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010482">
        <property type="entry name" value="TECPR1-like_DysF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31679">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31679:SF2">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06398">
        <property type="entry name" value="Pex24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00694">
        <property type="entry name" value="DysFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00693">
        <property type="entry name" value="DysFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Peroxin/Ferlin" evidence="7">
        <location>
            <begin position="228"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Peroxin/Ferlin" evidence="8">
        <location>
            <begin position="321"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="354" mass="40519" checksum="696BF224FDDDF5EE" modified="2017-01-18" version="1" fragment="single">QTKTESSPLLSSTPVTVSKILVKSYPYFVIADKLLSVLTWTNDDAWLSLLVVAVYATLVLYFESIVTYFGHILAVAILVAYSILSKHVETTISTKPTLDDIIQLLTSVTVKADLFLNPITSLALTSYDIKRLLFTTVFLSPLYVVITYFLLTPRTLLLLFGLYCLTYHSIFSRVTRKVLWKFRLVRLLSFYMTGLDFGGININKDSGIFAAVKKVNEKVGIKNKDGKPIVFTYVLYENQRRWLGIGWTPNLLSYERTAWTDEFLNESSPTDKFKLPETEEGSGMVWRWVDKTWRLDLTNDGALQLPSSKPKTTANPGPDDGFIYYDNTWKKPSTEDSFSKYTRRRRWIRTAELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9T1</accession>
    <name>A0A1E3P9T1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4 15">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_76353</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Endoplasmic reticulum membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4 15"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041382.1">
        <property type="nucleotide sequence ID" value="XM_019184367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9T1"/>
    <dbReference type="STRING" id="683960.A0A1E3P9T1"/>
    <dbReference type="GeneID" id="30201613"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23283">
        <property type="entry name" value="beta-trefoil_MIR_PMT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF50">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="168"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="595"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="635"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="664"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="692"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="331"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="394"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="463"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ62175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="779" mass="88388" checksum="CF2B01F53F033C9A" modified="2017-01-18" version="1">MAPKKTRQQTNEKSSHVEELDPVTEFVFEKGVDRPFLITTPPPNLSHLRSVTTFKERLFLAALVIFALFIRLQILYQPNSVVFDEVHFGGFAKKYILGTFFMDVHPPLAKLLFAGVASLAGFKGDFGFEKIGDVYPETTPYFWMRAFPAVLGVGTVVLLYLTLRASGVRPLVAFITGLIFTYENANVTISRYILLDSPLIFFISAAVYGAKRFENEIPFSFQWYRALVSTGVALGLALSSKWVGLFTVAWVGALNVWDLWFLIGDLTVPVCKIFKHTIARGLILLGIPLVLYLTFFAVHFKVLYKEGDGAAFMSSAFRSTLQGNKIPGNILADVGVGSVISIRHIDTQGGYLHSHDSLYETGSKQQQITLYPHLDTNNNWLVELYDATEAPTSFQQIQDGTKIRLKHILTGRRLHSHDHKCPVTENDWQKEVSAYGYEGFEGDANDDFVVEIQKAYTKSPDAQQNLTALQSVIRLRHAMTGCYLFSHEVKLPKWGFEQQEVTCATQGVLSKSLWYVEQNENPFLPADARRVNYKQPSLIEKTIEAHQRMWHINQNLNAPHNWQSLPNTWPLLSRGINYWGEDNRQIYLLGNAPTWWASTAVIVIFAVYAATQILRWQKGASVGVDRHIYNFNVQTFKYILGWIIHFAPSFLMGRQLFLHHYLPAYYFAILTLGHFLDIIVSYIFAKKRSIGYTLIIIFSLISFLFYVNYSPLIYGTPWTKAQCEASKLVDTWDYDCNKFHESLSDYNIPPKIFEPSESYGGAQPIASQQNVGGQQVLQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA74</accession>
    <name>A0A1E3PA74_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial import receptor subunit TOM5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_25886</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Tom5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041407.1">
        <property type="nucleotide sequence ID" value="XM_019181200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA74"/>
    <dbReference type="GeneID" id="30198446"/>
    <dbReference type="OrthoDB" id="3999796at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019603">
        <property type="entry name" value="Tom5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10642">
        <property type="entry name" value="Tom5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="49" mass="5443" checksum="3FFC829CEA659D7B" modified="2017-01-18" version="1">MFGGGQPLSEEEKKAHQAQTNNTVITSVYIAAALWATPIIIHFVQKQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAC6</accession>
    <name>A0A1E3PAC6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cardiolipin synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_17773</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041457.1">
        <property type="nucleotide sequence ID" value="XM_019180759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAC6"/>
    <dbReference type="STRING" id="683960.A0A1E3PAC6"/>
    <dbReference type="GeneID" id="30198005"/>
    <dbReference type="OrthoDB" id="10020554at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043337">
        <property type="term" value="F:cardiolipin synthase (CMP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006873">
        <property type="term" value="P:intracellular monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007006">
        <property type="term" value="P:mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000017">
        <property type="entry name" value="Phosphatidyl synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004570">
        <property type="entry name" value="Phosphatidylglycerol_P_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF60">
        <property type="entry name" value="CARDIOLIPIN SYNTHASE (CMP-FORMING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000847">
        <property type="entry name" value="Phos_ph_gly_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="159"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ62250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="24324" checksum="7B92A78C99536509" modified="2017-01-18" version="1" fragment="single">KITKPIKGLIPEHENIYTIPNILTFTRLVTAPIIGFLIVKQQVVYALALFTYSCITDFIDGYIARRFKMHSVVGSIIDPMADKFLMVTCTACLAHAGQIPLYLAVVILGRDVLLAISAIYYRYISLPPPKTFLRYWDFSIPSAEVHPTTISKYNTGFQMLYIGAAVVKPVLLSVIDPSYIELLEYCFSGFGYFVGTTTVLSGLSYVFSKDAVKILNQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAC9</accession>
    <name>A0A1E3PAC9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Methylenetetrahydrofolate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_87701</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041535.1">
        <property type="nucleotide sequence ID" value="XM_019186124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAC9"/>
    <dbReference type="STRING" id="683960.A0A1E3PAC9"/>
    <dbReference type="GeneID" id="30203370"/>
    <dbReference type="OrthoDB" id="41403at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004487">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009396">
        <property type="term" value="P:folic acid-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000012">
        <property type="entry name" value="Methylenetetrahydrofolate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF3">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="2">
        <location>
            <begin position="16"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="3">
        <location>
            <begin position="148"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="25946" checksum="37571745711F1DBC" modified="2017-01-18" version="1" fragment="single">MSDSTSKPAGRTILASNIAKGFISEVEEGVKQLGRSPKLVGFLANDDPAAQMYADWTGKTSKSLGFDYELRVVNKDDLEEAIIDANGDDKVDGIMVYFPVFGNRQDQYLQQVVSLEKDVEGLNHKYIQNMYHNVRFLDAEEKQKSVLPCTPLAVVKILEYLGVYNNLLAYGNRLYGKQVVVVNRSEIVGRPLAALLANDGATVYSVDLDGIQRFTRGEGLKLSRHQVVDVDLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAD2</accession>
    <name>A0A1E3PAD2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cysteine protease RIM13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_76362</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family. PalB/RIM13 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041391.1">
        <property type="nucleotide sequence ID" value="XM_019184370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAD2"/>
    <dbReference type="STRING" id="683960.A0A1E3PAD2"/>
    <dbReference type="GeneID" id="30201616"/>
    <dbReference type="OrthoDB" id="167576at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022683">
        <property type="entry name" value="Calpain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051297">
        <property type="entry name" value="PalB/RIM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46143">
        <property type="entry name" value="CALPAIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46143:SF1">
        <property type="entry name" value="CALPAIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00720">
        <property type="entry name" value="calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="7">
        <location>
            <begin position="140"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="709" mass="81500" checksum="E2AE9BB04D2AEA23" modified="2017-01-18" version="1">MEQDNVPKALRILERAKYQYALGATANAKKLGIEAINLLNSVLKDGAHDKVILKEICNDAIEFYQNISNSKYLTFDEKLTWLSSSVNGGFFPPAILKHSFIADEELNDELHLSEDQTAKLTSWKSINNDNDWSLDGIDHLYQDYLQDCSFVASLISLSKFNSKLLLNTIYPHKSSKRYGVTLHFNGCQRLVEIGDQLPVLSKECLYIKSRSNEHLLWVSLIEKAYLKVQGHGYEAKGSNASIDTYVVSGWIPQFIHADQRVDLNSLWEDLYDSFQNNRLVLSLGTGKHSEYYYPNHDYSILELVESTRSIVIKNPWEESIRAVKFEELFDHFETLYINWNPSNFIHTQMNFIWTYNDDIFFNNPQFSVTNDNPATVEVWILIEKFMGFEQSSINVLVYQSNGQKLFSKGKSLVYNSSNNSGFHLSKFKLGAGQSATIVVTNDSKQTQNFSIHAYSSKRVSLTKAKAQYPFLKSIKDEWDVDSCGGNWSHQSYVKNPQYQIEVPEAGQDVEVIFGLFSNKDVLLNLQLYWDDDKPFSEKASLIHEKYHAGSMTKCIKLKSGQKYKLVMSTYDTDILAPFRLLINSNVGLSIQKLSSRLGLFLRTFKFDWNNKNRFKLKFQVKRETPVKVHIWTDNLYSSYRPKIRASLFYSDGRPIQINTEFDDSIYGIWLKKAIISPTDSVILLIERFEVGNDEMSIEIGSDQKIEVLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB15</accession>
    <name>A0A1E3PB15_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_60673</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041822.1">
        <property type="nucleotide sequence ID" value="XM_019183032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB15"/>
    <dbReference type="STRING" id="683960.A0A1E3PB15"/>
    <dbReference type="GeneID" id="30200278"/>
    <dbReference type="OrthoDB" id="1661054at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007255">
        <property type="entry name" value="COG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21311">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21311:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04124">
        <property type="entry name" value="Dor1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="365"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="383"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="395"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="439"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ62615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="578" mass="66258" checksum="31433E6024F0CA4B" modified="2017-01-18" version="1">MTEVENYDASSLEVLLQELSDDLPSEIQSLLESPALHSDILKYLNDISSIESNDYLSSIPPTPAANRDGNIVEERTLVQDIAELEANQRSIDSKLKNLIIKSQGSIIENEIGLKDAYQSFDTEFAHNAKSLWRTFKDDDIDEIKDDQDQELQELEFDLDPTTKQDTFYQTISNIKNTQLNETEKHKNLSIVLDNMDQILNVLELPSLAGACIKAGYYSEALEISSYTRRLAIRFPNSDLVKEVEAGVKSEMSMMLTGLIRLLRTNLKQSSIIKILSYLRRIQPFSQSNNADEQLKRILLHSRYEFIKLELSSLLPLRESALHEKYLKRSIEVIREFCFGSIMTYKSIFADIDDFELEDVKIELEEVEQSEEQIPKVEKEEEVSTQAEASETKANNAKEEGDAAEKEFNDESKLDADSKDQKVEEGSEDNKEEDVPADSLEQKQDENKQTEQKDKSTDDADELKELKEPTPEKPIIKLLENRKQSNILLFEFVRNVLIELIQNLKVSLLEVHEKSIRDGLYLQLIYCSQSLGRIDENFSDLMTVTLLNAKDENSKHIIDRESWAVSIQKQQQLAKSLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB90</accession>
    <name>A0A1E3PB90_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HORMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_60732</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB90"/>
    <dbReference type="STRING" id="683960.A0A1E3PB90"/>
    <dbReference type="GeneID" id="30200294"/>
    <dbReference type="OrthoDB" id="21254at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016035">
        <property type="term" value="C:zeta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003511">
        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045091">
        <property type="entry name" value="Mad2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842:SF10">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02301">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HORMA" evidence="2">
        <location>
            <begin position="19"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="123" mass="14890" checksum="E238229653E601B6" modified="2017-01-18" version="1">MTKIIPKKKPDGSLSLTPNNLYFTFQQFLVTWINIITKERSIYPEESYEFRNSFNLQCPYNRHPDVTKWNDDLISDIMDDLCFKKIKLISLIIFDKETEETIERYSLNMFEFLHTPKDYYDKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBB4</accession>
    <name>A0A1E3PBB4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mRNA transport regulator MTR2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_66934</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041910.1">
        <property type="nucleotide sequence ID" value="XM_019184065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBB4"/>
    <dbReference type="STRING" id="683960.A0A1E3PBB4"/>
    <dbReference type="GeneID" id="30201311"/>
    <dbReference type="OrthoDB" id="25408at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019488">
        <property type="entry name" value="Nucl_pore_RNA_shuttling_Mtr2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10429">
        <property type="entry name" value="Mtr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="18775" checksum="48AF904B9D19E728" modified="2017-01-18" version="1">MVDQGTQLEAYIKELFQTLDKQFSPQLNLDSYKAQFLPQLKLNSPVILNGNPIGGKLAFQEHWLKFPGSQHNITSLDYHSIPGTGTFVINLSGKVRFDETGKTKLGETADIGGPAPVPSIGGAPRTIWGSWLGFNANLILDEAALQNPNSEVINSLNWKIVYRPEDALVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBF6</accession>
    <name>A0A1E3PBF6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor ISY1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pre-mRNA-splicing factor isy1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_88362</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ISY1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041837.1">
        <property type="nucleotide sequence ID" value="XM_019186146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBF6"/>
    <dbReference type="STRING" id="683960.A0A1E3PBF6"/>
    <dbReference type="GeneID" id="30203392"/>
    <dbReference type="OrthoDB" id="1739576at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071020">
        <property type="term" value="C:post-spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000384">
        <property type="term" value="F:first spliceosomal transesterification activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000389">
        <property type="term" value="P:mRNA 3'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.660:FF:000001">
        <property type="entry name" value="pre-mRNA-splicing factor ISY1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009360">
        <property type="entry name" value="Isy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037200">
        <property type="entry name" value="Isy1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13021">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ISY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06246">
        <property type="entry name" value="Isy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140102">
        <property type="entry name" value="ISY1 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="134"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="279" mass="32758" checksum="089FA6523A99434B" modified="2017-01-18" version="1">MSRNSDKANTVLFRYQEQKAEEAGYIDYNSTARPRSVQKVTNLKDAENWRKQVLKEISQKVTKIQDEQLSSYQIRDLNDEINKLMREKYAWEFHIKDKLGGTDYLRSAGRFNEGGVLIRGYRYFGRAKELPGVKEILEKQQQDLKDQKTNQENAKSQKQKIKELESRVNLEYYGYFDEKNSKAGDSLGDNVWDEVNQILGDEITVPKPMEIESDEKEMLTDELLKFERSATRKRSKKLGNIQDPKEITIIENSEPPSQQQVEAYLVERRRKQLSEKYNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBI3</accession>
    <name>A0A1E3PBI3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_25771</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041454.1">
        <property type="nucleotide sequence ID" value="XM_019181188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBI3"/>
    <dbReference type="STRING" id="683960.A0A1E3PBI3"/>
    <dbReference type="GeneID" id="30198434"/>
    <dbReference type="OrthoDB" id="29596at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990391">
        <property type="term" value="C:DNA repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062128">
        <property type="term" value="C:MutSgamma complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062037">
        <property type="term" value="F:D-loop DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051026">
        <property type="term" value="P:chiasma assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF20">
        <property type="entry name" value="MUTS PROTEIN HOMOLOG 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="5">
        <location>
            <begin position="447"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="618" mass="70210" checksum="979CC63224A123D9" modified="2017-01-18" version="1" fragment="single">TFDSSDTVYVEPETLYALQILKENDSGLNKKDSHHLQSLFGMLNQTHSALGYKRLRSWFYAPLANISLINKRLNTVEVLISEDNTQYLENLMCFQKQLPNINQTISSIEMGRPSVKSWSQMASFLKYAIEIYDVVGLMKDIEFVEVLQNILTEVDVEQLRNLCTAVDSAIDFDQSKQWKRVYVRDGLVNHLDCCRKNYDRLETTLEEAASEISIRYPHVPSTFLNVVYLPQVGYLISIDITNRDEVPEEWEEVFATVTNMYYKDEYTHGMDDELGDVYQLIIDLEIEVLYELQSGIFDSMDMLLTAGELFAELDCFMSLASISMLQGYVKPEIVDESVIEIKNGRNPIFENLVPSYIANNTDIEKHQLCIVTGANGSGKSLYLLQVGIIVYLAHLGCFVPAQFAKIGLTDKILSRIFSMESLEKCESSFGLDLQKMSKCLQLGTEKSLLLIDEFGKGTDVIGGPALLGAIIENLSNSETPPRTIITTHFHELFKNNIIELSSKVKHNHMSVIFQQQDGEDKLTYLYELKDGLALNSFGIDCAKACGIPNNIITRANEILRESHESLISSVDSSKYRDIEQAKYIAREFLTWDLETEESQTEQELKEHLNNTLHVINTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBK0</accession>
    <name>A0A1E3PBK0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_27146</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the formin homology family. BNI1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041539.1">
        <property type="nucleotide sequence ID" value="XM_019181343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBK0"/>
    <dbReference type="STRING" id="683960.A0A1E3PBK0"/>
    <dbReference type="GeneID" id="30198589"/>
    <dbReference type="OrthoDB" id="1104827at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005522">
        <property type="term" value="F:profilin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070649">
        <property type="term" value="P:formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2220:FF:000006">
        <property type="entry name" value="Cytokinesis protein sepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051661">
        <property type="entry name" value="Actin_filament_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47102:SF1">
        <property type="entry name" value="BNI1-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47102">
        <property type="entry name" value="PROTEIN BNI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="5">
        <location>
            <begin position="31"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="444"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="408"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="495"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00037935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="519" mass="60534" checksum="59F625BCB555C7FD" modified="2017-01-18" version="1">MLSLKSNPESKKAAFKPLSNILTQKKIDEISKSLHRPSKKLKQLHWEKIDKVDQTLWGESTHSRTDELMELGILKEVEDLFTIKETKKKVSPSSATAKTDKITFLPRDLSQQFGINLHMFSNFTEEQLISKVIKCDYDVLNNISVLEFFNKDELNEIGNGLLRNFKPYSTDFVTGSKPEKDVTVLERSDRIFLELCYNLRFYWRSRSRALLMIKTYEKDYYDLSKRIQTLDDAVKSVKNSKNLKNIFILIREIGNFMNNKPVEGFKLASLQKLNFIKDQNQNTFLQYLEKVIRRAYPEYLKFMEELSSLQDSSKISVEQLSVDIDAYIKTIKSVERSVESGNLSDPKKLHPDDRIVTKVLPRLPEAKRKYQLLEDQQKFVISDFEKLMKYFGENPSDAGSKASFFQKFQEFMNDFKRVQKQNHEEEQKIKIYEKRKAMMLMNKKAREESRGSVTTDGESDVDGKQEEDVVDNLLRKLKGVTNERRTSTTTSSRSRNMDQEDTKLLTRAQTLLEGTKNIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBW3</accession>
    <name>A0A1E3PBW3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Serine/threonine-protein kinase TOR</fullName>
            <ecNumber evidence="15">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_24841</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Vacuole membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the PI3/PI4-kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="15"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042113.1">
        <property type="nucleotide sequence ID" value="XM_019181062.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3PBW3"/>
    <dbReference type="GeneID" id="30198308"/>
    <dbReference type="OrthoDB" id="381190at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010972">
        <property type="term" value="P:negative regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016242">
        <property type="term" value="P:negative regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901992">
        <property type="term" value="P:positive regulation of mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031137">
        <property type="term" value="P:regulation of conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905356">
        <property type="term" value="P:regulation of snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05169">
        <property type="entry name" value="PIKKc_TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000020">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.150:FF:000001">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000371">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000004">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.150">
        <property type="entry name" value="FKBP12-rapamycin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009076">
        <property type="entry name" value="FRB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036738">
        <property type="entry name" value="FRB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057564">
        <property type="entry name" value="HEAT_ATR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024585">
        <property type="entry name" value="mTOR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026683">
        <property type="entry name" value="TOR_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF9">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08771">
        <property type="entry name" value="FRB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23593">
        <property type="entry name" value="HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11865">
        <property type="entry name" value="mTOR_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01346">
        <property type="entry name" value="DUF3385"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01345">
        <property type="entry name" value="Rapamycin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47212">
        <property type="entry name" value="FKBP12-rapamycin-binding domain of FKBP-rapamycin-associated protein (FRAP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="FAT" evidence="17">
        <location>
            <begin position="1236"/>
            <end position="1789"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="16">
        <location>
            <begin position="1963"/>
            <end position="2278"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="18">
        <location>
            <begin position="2308"/>
            <end position="2340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU364109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ62906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="2340" mass="265416" checksum="22FC271E8AF805A2" modified="2017-01-18" version="1">MSQAPDSFALSQIFLGLKSKNAQERAAAVVELRNHLVTTAREVSSEQFTRYNNEINNKIFELIHGSDPSEKVGGILAVDMLIDFFGGEDLPNQTSRLANYLRFLIPSTDVEIMKLAAKTLGKLAIPGGSLTSDFVDHETRNAIELLQTEKQESKRHAAVLIIAAIAENSPTLFHSYVNTILENIWSALRDSKLIIRIDAAKALEKCLDVIYERDLNLRKNWFNKLIEGASAGFKMNTSEAIHGSLLVFKELVKKGGMFIQDKYDAIFETSFKFREHKYNIIRQEVTHIIPLLAAYNPPKFVEKYLHRTLLFYLSQLKEENEKAAILISIGDIAYEVEGQITTYLDAILDNIRDGLNSKSRARRDIEPAIFYCISKLASAVGPALTKHLQRDILDMLLSCSLSDHMRECLLVLTQRIPPLEQTINTKTLNLLSYCLSGYSFSQPGSPNSHNIMNAQLARQYRDATASRNGINNDDYIIVQALKMLSFFNFKNESLFEFVRYCTITYIEHDNTEVRKIAALTSCDLFLKDSVCFQTSSYSLNAVSEVLDKLLTVAITDPVPDIRLEVLNSLGKNFDPQLSQADNVRLLFMALNDEFFAIQKVAIQIIGRLSSINPAYIVPFLRKTLIQLLTKLEYSTASRKKEESAILLSLLISSSKNITKPYVKPIIDVLLPKARDKSASVAASAIAALGELTVVGGEDVKPFISDLMPLILETFQDQSSSFKRDSALKTLGQLSGSTGYVIQPLLDYPQLLGIIINILKSETSPNIRRETVRLLGILGALDPYKHKEVERSSKHIVVEGPPTDVALLMQGLSPSNEEYYPTVVISTLLKILKDPSLAAYHSSCIQTIVLIFKTITITCIKFTGSIIEAIISVMHTCPPSMLEFYFQQISALISIVKQHIRPFLPEVFDTIKEYFHLANLQVTIISLIESIAKALDGEFKTHIPSVLPLLLDALGNDKTTNVSLRILKAFVVFGGSIEEYIHLIIPNVVHLFEYGPQNVRKAAIITVGKISRNVNLNDISSRIVHTLLRVLSTRNDELVKASMNTLSLLLLQVSNEFIVFIPSITKVLFKYNIESPVYDQLVNKLLNDEPLPQNLILDKEPESGDGSDIVELPSKKLPVNQQILKNAWQCSQCRTKEDWQEWSRRLSVQLLKESPSHALRACSALAGSYYPLAKDLFNASFASCWSELYTQHQEELVQSLCMALSSPNNPPEIYQTLLNLAEFMEHDDTALPIHISTLGQYAQRCHAYAKALHYKELEFSQEPSTPTIESLISINNQLQQSDAAIGILKHAQQHHDLQLKETWFEKLQRWEDALKAYNEREVDTIDTTMGKMRCLHALGEWDQLSQLAQEKWTNSSTDIKRTVAPLAAAAAWGLGNWERMDTYITVMKPESPDRSFFNAILCLHRNNFGEASSHISKARDLLVTEITALVSESYSRAYGVVVRVQMLAELEEIIKYKNLPQHSDARAVMRKTWNARLLGCQRNVDIWQRLLKVRALVVKPKQDMDVWIKFANLCRKSGRLGLAEKSLNSLLEEGDDPNTSRAPPQVVYAQLKYMWATGSQREALSHLIDFTSRMSHDLGLNPDDLITQPLPTHTTIKNVQEYTRLLARCFLKQGEWQVALQPNWRTENSETILGSYLLATHFDSKWYKAWHNWALANFEVISLLTSKSAINQNDENENAYTQELIQQHVVPAIRGFFHSISLSESNPLQDTLRLLTLWFTFGGLPEAAQAMSEGFSMVKIDTWLEIIPQLISRIHQPNQTVSRSLHGLLSDLGKAHPQALVYPLTVSVKSDSVSRQRAASTILDKMRIHSPTLVGQSELVSDELIRVAVLWHELWYEGLEDASRLFFGEHNVDKMFAVLDPLHKMMEKGPETLREVSFQNTFGRDLSHAYEWVLSYRRTKDISKLNQAWDIYYNVFRRINRQLPQLQTLELQYVSPKLLAAKDLELAIPGTYRPGKPIVSILSFEPSLTVISSKQRPRRFGIKGSDGKEYFYLLKGHEDIRQDSLVMQLFGLVNTLLVNDPECFKRHLDIQRFPAIPLSPKTGLLGWVPNSDTFHVLIREYRESRNIILNIEHRIMLQMAPDYDNLTLLQKVEVFTYALDNTRGQDLYKVLWLKSRSSESWLDRRTTYTRSLAVMSMVGYILGLGDRHPSNLMLDRITGKVVHIDFGDCFEAAILREKYPEKVPFRLTRMLTYAMEVSGIEGTFRITCEHVMRVLRDNKESLMAILEAFAYDPLINWGFDLPTQAIADATGIKFQLVNTAELLRRGQIDEQEAAKLEVQQKIEIRNARASLVLKRITDKLTGNDFRRFNELNVSSQVDKLIQQATSVENLCQHYIGWCSFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC28</accession>
    <name>A0A1E3PC28_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome biogenesis protein YTM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">YTM1</name>
        <name evidence="9" type="ORF">WICANDRAFT_77021</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat WDR12/YTM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042067.1">
        <property type="nucleotide sequence ID" value="XM_019184489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC28"/>
    <dbReference type="STRING" id="683960.A0A1E3PC28"/>
    <dbReference type="GeneID" id="30201735"/>
    <dbReference type="OrthoDB" id="10251381at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110136">
        <property type="term" value="P:protein-RNA complex remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000706">
        <property type="entry name" value="Ribosome biogenesis protein YTM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03029">
        <property type="entry name" value="WDR12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012972">
        <property type="entry name" value="NLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028599">
        <property type="entry name" value="WDR12/Ytm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855:SF11">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN WDR12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855">
        <property type="entry name" value="WD40 REPEAT PROTEIN 12, 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08154">
        <property type="entry name" value="NLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NLE" evidence="8">
        <location>
            <begin position="7"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="137"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="196"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="272"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="359"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="444" mass="49050" checksum="D5BEB3B126598680" modified="2017-01-18" version="1">MSDKSQIKIKFFTREEDESLQVSENPLYVPISLKRYGLSEIVNHLLETETPVPFDFLINGTLLRTSIDEYLTENALSSEAFLTLEYTRSVLPPSFLASFNNEDWVSSLDVKHNQILSGSYDGIVRTWDLSGKVQKQFSGHSGAIKAVRWISETRVASAGNDRQIRLWKASATDSNGIRDLDEDEDEPEEGKTLAILEGHKAAISSLAVNNNRILSGGYDNAIGFWSTNYKEMTSIDPLADPNNNVTSTAAKKRRKLALQDASIRRRAPLSFLEGHSQPVEDLIFAPNDPSVAYSVSQDHTIKTWDLVTSRPVDTRTTGFSLLSITSLPKLNLLACGSSARHITLHDPRASATTVTQSQLIGHTNFVVSLAASPDNDYMFSSGSHDGTVKVWDIRAQKALYTITRESKAPKGQDKVFAVSWDQTGIISGGQDKKIQINKGTQLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC92</accession>
    <name>A0A1E3PC92_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bul1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_26456</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042246.1">
        <property type="nucleotide sequence ID" value="XM_019181264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC92"/>
    <dbReference type="STRING" id="683960.A0A1E3PC92"/>
    <dbReference type="GeneID" id="30198510"/>
    <dbReference type="OrthoDB" id="3977431at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039634">
        <property type="entry name" value="Bul1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022794">
        <property type="entry name" value="Bul1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007519">
        <property type="entry name" value="Bul1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904:SF1">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04426">
        <property type="entry name" value="Bul1_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04425">
        <property type="entry name" value="Bul1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bul1 N-terminal" evidence="2">
        <location>
            <begin position="23"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Bul1 C-terminal" evidence="3">
        <location>
            <begin position="474"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Bul1 C-terminal" evidence="3">
        <location>
            <begin position="601"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ63039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="683" mass="77148" checksum="3CA0DB56142F1863" modified="2017-01-18" version="1">MYRAFQFTPSKDSAESSSHYEEPPNYTNPQNDSGSDSEPFTDGSSTRLTTPTSALSESNANSPAAGSFEEFESSYADKVHTLPHADFKELEVSINVTKDAPIDTKKSYEKEALLKEYTSGDIVHGNVIIHNTSDKPIQFQMFHVTLEGYITIVDKDNKKQFIKRFLTMVDMSASWSHGCVSPAANISFDPLSKDEEGCVLGLRYDRIIEAHTKHKKFFAFKFPYSLLDNVCRHQQEVHSLLPPSFGVDRVKSNGKFSRIPFSPALNYGHLGTHGSPILTNDISEAKLCVSYSINAKVIGSSQKIPTALCVWKEEEYALRFIPFGFAVPLFSSKKCLDSMVQSIEHGFQMASRALKLKVEGKLDEVRRFDLEIKHRQLRVSSNDYAGNQTQSFPLRNKSFGGSDFSKVEAHLKYLYPRSTSSFLTARVKANQTGITKSGLIKISTTIPKDGLPYKSPKLLKKINEVSNLSETGLKNYDVLSSSLSMTEKQKLTKLRLYLNFTPSDNSLEVAPPPIKFVKTTLLVFNIYSSSPIPIKLSQDLFLEEKNLEQLKSKFDGYYKAFTELQSELKSNSIEINSYLDNSILQDIISMKDLQVDKFSIQAFDSSIDESTWNLSKKAEDRRIWERDITLSFTFKEKMSETLIPNFQSCILSRVYSIGVKFGFKNGQSCELFVPIRIRKFDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC93</accession>
    <name>A0A1E3PC93_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_26051</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042188.1">
        <property type="nucleotide sequence ID" value="XM_019181222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC93"/>
    <dbReference type="STRING" id="683960.A0A1E3PC93"/>
    <dbReference type="GeneID" id="30198468"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF515">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE RTK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="41"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="342"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="347"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ62981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="378" mass="42671" checksum="7AF1F23778E33A15" modified="2017-01-18" version="1">MSSQGSTPPPPNGAYTTASSAASNKLLFGTDNAGELLEKYGIPGKVLGEGAGGSVSVVERSDGKLYAVKKFRPKNDRESEADYSKKVTSEFCIGSTLKHQNVIETLDMLKEGLNFLVVMEFAPFDFFTIVMSGLMTKHEIFCCFKQITNGTSYLHSMGLAHRDLKLDNCVVSNQGIIKLIDFGSAVVFKYPYENEIVRAKGVVGSDPYLAPEVLTERYYDPRPVDVWSIAIMFCCMTLRRFPWKAPKQTDNSFKLFSTKPEINPENPNHKSRSKGPYRLLRLLPHASRDLIAAMLQLKPEDRLLIDQVVNNEWFKSIECCQVGQDGELVTKPENHKHHLVTEDELNEIQKKKEQQKKREEERINNERQNQSTTVKVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCE9</accession>
    <name>A0A1E3PCE9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">COX assembly mitochondrial protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_25476</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for mitochondrial cytochrome c oxidase (COX) assembly and respiration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the CMC family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042167.1">
        <property type="nucleotide sequence ID" value="XM_019181151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCE9"/>
    <dbReference type="STRING" id="683960.A0A1E3PCE9"/>
    <dbReference type="GeneID" id="30198397"/>
    <dbReference type="OrthoDB" id="532630at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013892">
        <property type="entry name" value="Cyt_c_biogenesis_Cmc1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08583">
        <property type="entry name" value="Cmc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="101" mass="12094" checksum="B5583781F9D5D8DB" modified="2017-01-18" version="1">MHPQVHAPRFISCVDYIEALEKCHQQEYLKRCFGICNNQKEALSKCLHEARLETQKHLILKSREKQKGFRESWKKMDEEAYGEEEFLKKLLQREKAKRGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCG7</accession>
    <name>A0A1E3PCG7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribose-phosphate diphosphokinase</fullName>
            <ecNumber evidence="4">2.7.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_61024</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">5-phosphoribose 1-diphosphate synthase involved in nucleotide, histidine, and tryptophan biosynthesis. Active in heteromultimeric complexes with other 5-phosphoribose 1-diphosphate synthases (PRS2, PRS3, PRS4 and PRS5).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="4"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042187.1">
        <property type="nucleotide sequence ID" value="XM_019183099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCG7"/>
    <dbReference type="STRING" id="683960.A0A1E3PCG7"/>
    <dbReference type="GeneID" id="30200345"/>
    <dbReference type="OrthoDB" id="413572at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000014">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000056">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF36">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="14">
        <location>
            <begin position="5"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="115"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="130"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="184"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ62980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="460" mass="50563" checksum="42544C40C36EC09C" modified="2017-01-18" version="1">MVHDIVVFSGSSHPGLAERICHNLSLDKGKVKLGKFSNGETSISVNESVREKDVYVVQSGCGHVNDNFMELLIILSACKTASAKRVTAVIPLFPYSRQPDIPYTKKGAPLVSKPKSEYTFESVPNTPRANSRNSNFESSSFETPQKEIQPSELIHQQPPPSFNLEQPTPKNHHHHPSRIPKIPETNQNSTSDGNTKPISINSGYKQWVAQAGTLVADLLTCAGADHIITMDLHDAQFQGFFDIPVDNLYSKPILQHYISSKIPNYQDAIIVSPDAGGAKRATAIADTLGMSFALIHKERRSHQSSRHPSNVNSNNELKSVALQPNLSSDSGFQITTVEKKLKYATTMLVGDVKGKECILIDDLVDTSYTITRAAKLLKDQGAKKVYALITHAIFSGDALKRVKASAIDKLIVTNSVPQEDHQKELGDALEVLDVSRIFGEAIRRIHNGESVSMLFDHGWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PD94</accession>
    <name>A0A1E3PD94_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ERCC4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_82769</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042054.1">
        <property type="nucleotide sequence ID" value="XM_019185437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PD94"/>
    <dbReference type="STRING" id="683960.A0A1E3PD94"/>
    <dbReference type="GeneID" id="30202683"/>
    <dbReference type="OrthoDB" id="361020at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905348">
        <property type="term" value="C:endonuclease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006277">
        <property type="term" value="P:DNA amplification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000736">
        <property type="term" value="P:double-strand break repair via single-strand annealing, removal of nonhomologous ends"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000710">
        <property type="term" value="P:meiotic mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000715">
        <property type="term" value="P:nucleotide-excision repair, DNA damage recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20078">
        <property type="entry name" value="XPF_nuclease_XPF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000009">
        <property type="entry name" value="UV endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006167">
        <property type="entry name" value="XPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047520">
        <property type="entry name" value="XPF_nuclease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00596">
        <property type="entry name" value="rad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150:SF0">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="7">
        <location>
            <begin position="731"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="955" mass="109056" checksum="C9E639B3EAD22D3C" modified="2017-01-18" version="1">MPLFVKDDEEETDELVLNLNSVKDDNKPEEQVNEDEQAEYPLIEKEEEDLVEQIEPVNISLTLPLKFQQLILEDLVSDDSLLVLGKGLGLEPIAANLLHTLSAPATIANVTKRSLVIVLGANDEENEKISEELTELSWIDDGAEDSKRPFVVIKSDQYSSDKRQKLYQSGGIMSVTTRIFIVDMLSGIVDPSKITGIVVLHAERVNDLSNESFIVYMYRQKNKWGFIKALSDTAEEFSSGFSPLHKKLKDLRLKKILLWPRFHIEVTSSLLTKDTKTVTEVRVSMTESMKHIHTGLLGCLQKCIEELKRKNPTLAIEYWNIENALDPHFLGIIHMILDPNWHRISFDSKQLVKDIKLLKDLLRCLISYDAVDFYEMIQLVLEANKPSVDKRNTESPWLMADESQAVIAYAKKRVFANNEYVLEEAPKWEQLAILLDDIVLERSHNPQKDGPVLIMCSDSKSAFQLKRYISTMKESKDTQRSFSARKIMVDKLQAYLDSKETKATVTSKIQEAAAKENEDQELHISRAFARGPVTSKRRRTRGGAAVAAVGRLMSAPVAGDDIESKMSKEDVEMELFELDAKILEDVEVLKEQTPSTFFEYIDRENQVIIEKYSSKTNDLILQELMPSYIIMYEPDLAFIRRVEVHQASYKDKGAAKTYFMYYSDSAEEQKHLTAIKKEKEAFTKLIREKSALASHFETDEDFSKFSIRKSQVMNTRIAGGSTFRTSTDEARVIVDTREFRAPLPGLLHRIGLRVVPCMLTVGDYIVTPKVCIERKSIPDLIGSFKSGRLYEQCERMSKYYELPALLIEFDNNQSFSLEPFSETRVRPNSVQAPHPSSSSRMQDEIQMNLAALVLKYPKMKILWSSSPYQTTELIMELKASREEPDIVKAVNAGLKEDKQRANDDTSITNIDFYSIMRKVKDTSKLVEMTRDELVQTVGEDLGEKIYDHIQANYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU56</accession>
    <name>A0A1E3NU56_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Copper-fist domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_107852</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454224">
        <property type="protein sequence ID" value="ODQ56658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035865.1">
        <property type="nucleotide sequence ID" value="XM_019180511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU56"/>
    <dbReference type="GeneID" id="30197757"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.430.10">
        <property type="entry name" value="Copper fist DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001083">
        <property type="entry name" value="Cu_fist_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036395">
        <property type="entry name" value="Cu_fist_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00649">
        <property type="entry name" value="Copper-fist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01090">
        <property type="entry name" value="Copper-fist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57879">
        <property type="entry name" value="Zinc domain conserved in yeast copper-regulated transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50073">
        <property type="entry name" value="COPPER_FIST_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Copper-fist" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ56658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="286" mass="32179" checksum="0BD03D8B8421B61C" modified="2017-01-18" version="1">MLYLGVLRFCCWKCRSGHRQLQCTHYDRAEKSLVFNVGQQGRPALDEERRVFKLVAADFDAEANPKCSESKLYKFQCRKSPRFRCCNGDSLSIDIYLDPKKSKVVEIHPPMEIHSGSNGISVDAFQLPRRNGKAKFINPDSSKIKDYKSTQSVDTDIIAEKKTHPLNVKLLPPPLSTKPPSTSPVNATRGRLNPKTQSATKRTIEREDTPIPSSSLSLSPSPSSQKRPRTSSKQTQTDPVKELSSPNLPSQNTRSHAFENISNHVPSTPMEPLRTRITRRRSKSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUE3</accession>
    <name>A0A1E3NUE3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_98192</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454216">
        <property type="protein sequence ID" value="ODQ56704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035911.1">
        <property type="nucleotide sequence ID" value="XM_019186642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUE3"/>
    <dbReference type="GeneID" id="30203888"/>
    <dbReference type="OrthoDB" id="10544234at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133502" evidence="1">
        <location>
            <begin position="19"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ56704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="83" mass="9152" checksum="8703ABAE92049C79" modified="2017-01-18" version="1">MKFLPIVALSALFMNVQAQSLTGSYITSCETPTDTTQLLSVETGVKGTNVLILTQYAVTRYFDTCNGNTWSQEVTSTEYTEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUF6</accession>
    <name>A0A1E3NUF6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="6">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_37133</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the class-I DAHP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="6"/>
    <dbReference type="EMBL" id="KV454222">
        <property type="protein sequence ID" value="ODQ56660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035867.1">
        <property type="nucleotide sequence ID" value="XM_019182522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUF6"/>
    <dbReference type="STRING" id="683960.A0A1E3NUF6"/>
    <dbReference type="GeneID" id="30199768"/>
    <dbReference type="OrthoDB" id="4699125at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000005">
        <property type="entry name" value="Phospho-2-dehydro-3-deoxyheptonate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006219">
        <property type="entry name" value="DAHP_synth_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00034">
        <property type="entry name" value="aroFGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009395">
        <property type="entry name" value="PRK12755.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE DAHP SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225:SF18">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE, PHENYLALANINE-INHIBITED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001361">
        <property type="entry name" value="DAHP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP synthetase I/KDSA" evidence="7">
        <location>
            <begin position="51"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ56660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="366" mass="40137" checksum="B82728816147DCB9" modified="2017-01-18" version="1">MFIQNQHIGDRTRLEDWRITGYDPLTPPDLLQHEFPLDEVNKKVILEGRDDAIKILNGQDDRLIIVVGPCSIHDPKAALEYQAKLKKIVDEVKGELLIIMRAYLEKPRTTVGWKGLINDPDIDNSFQINKGLRIARELFVNLTKTLPIAGEMLDTISPQFLSDLFSLGAIGARTTESQLHRELASGLSFPVGFKNGTDGSLTVAIDAMRSAAHSHHFLSVTKPGIVAIVGTEGNQDTFVILRGGKSGTNFDEESVKKAQSDLIAQGITSKPRIMVDCSHGNSNKNHRNQPKVAHEVAEQIKKGSESICGLMIESNLVEGRQDVPASGKQDLVYGCSITDACIDVDSTEAVLKELAEAVKARRALKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUK8</accession>
    <name>A0A1E3NUK8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_71357</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 31 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036023.1">
        <property type="nucleotide sequence ID" value="XM_019184240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUK8"/>
    <dbReference type="STRING" id="683960.A0A1E3NUK8"/>
    <dbReference type="GeneID" id="30201486"/>
    <dbReference type="OrthoDB" id="10257739at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1340">
        <property type="entry name" value="Mediator of RNA polymerase II, submodule Med31 (Soh1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038089">
        <property type="entry name" value="Med31_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008831">
        <property type="entry name" value="Mediator_Med31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13186">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05669">
        <property type="entry name" value="Med31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0010">Activator</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ56816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="93" mass="11409" checksum="59ABF82238ECFD51" modified="2017-01-18" version="1">MELPTRWEVELEFVQALANLQYLNFLAQNKYLQDSNFLHYLKYLEYWRKPEYAKYLVYPNCLHILTLLQTEQFREQILRADVAGVIMNDMCRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUV0</accession>
    <name>A0A1E3NUV0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein HRI1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_65234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036178.1">
        <property type="nucleotide sequence ID" value="XM_019183875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUV0"/>
    <dbReference type="STRING" id="683960.A0A1E3NUV0"/>
    <dbReference type="GeneID" id="30201121"/>
    <dbReference type="OrthoDB" id="4045395at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.320">
        <property type="entry name" value="Protein HRI1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031818">
        <property type="entry name" value="Hri1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043047">
        <property type="entry name" value="Hri1_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16815">
        <property type="entry name" value="HRI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ56971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="21705" checksum="ECA523D649D73B17" modified="2017-01-18" version="1">MTGKAKPIPNTNKVKYQQEINSVSPWIPGSLAASFNSVPNNDFVYEKIGEMQNPDTHKIQPYREIWRDIDPLKSDAYDFIGKDPQNNNHGAKIPCFVLKVVKRDNVEGTVIRVGNLIQGALFNTRSGETKAARYSLIEGEWRRCCSINSYRYMEDEPKIFKHVRLPTGVEAGVNIINRDGFEWEMIETNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV40</accession>
    <name>A0A1E3NV40_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HhH-GPD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_36286</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alkylbase DNA glycosidase AlkA family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036215.1">
        <property type="nucleotide sequence ID" value="XM_019182416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV40"/>
    <dbReference type="STRING" id="683960.A0A1E3NV40"/>
    <dbReference type="GeneID" id="30199662"/>
    <dbReference type="OrthoDB" id="415889at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032993">
        <property type="term" value="C:protein-DNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032131">
        <property type="term" value="F:alkylated DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043916">
        <property type="term" value="F:DNA-7-methylguanine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000004">
        <property type="entry name" value="DNA-3-methyladenine glycosylase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051912">
        <property type="entry name" value="Alkylbase_DNA_Glycosylase/TA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43003">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43003:SF5">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="5">
        <location>
            <begin position="135"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ57008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="336" mass="37849" checksum="1806E352C9AEBB09" modified="2017-01-18" version="1">MSAAKRTLRSASIVRDFPQDGDKLGPINDEDIPSTPKKKRVRQSATPKSPKKKTSFKQREAELLLQYPIPSDLSLPESFKSLHQPEFVKGLKHVLTIDPSLYPLIVSQPFGPFHPSEKPSNTDQGHFEKLCRSVIGQQVSGAAARSIEAKFVGVFNDKGQFPTPQEVINTKDETLRSAGLSVRKTEYIKSICQNFIDGEISQKIFENCSDQDILEKLVSIKGIGEWSAKMFLTFALHKLDVFASDDLGVARGISRYLESRPEILQKAQKEVDTSIQKKKSKFDDGKKRDWKIIHDLYVQHIAKDYAPYRSLFMLICWRASSTNVDVLSETSTTTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVG9</accession>
    <name>A0A1E3NVG9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Activator of Hsp90 ATPase AHSA1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_36611</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036302.1">
        <property type="nucleotide sequence ID" value="XM_019182454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVG9"/>
    <dbReference type="STRING" id="683960.A0A1E3NVG9"/>
    <dbReference type="GeneID" id="30199700"/>
    <dbReference type="OrthoDB" id="567237at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.20">
        <property type="entry name" value="Activator of Hsp90 ATPase Aha1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036338">
        <property type="entry name" value="Aha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015310">
        <property type="entry name" value="AHSA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 HSP90 CO-CHAPERONE AHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009:SF15">
        <property type="entry name" value="HSP90 CO-CHAPERONE HCH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09229">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01000">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103111">
        <property type="entry name" value="Activator of Hsp90 ATPase, Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Activator of Hsp90 ATPase AHSA1-like N-terminal" evidence="2">
        <location>
            <begin position="13"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="142" mass="16570" checksum="5577CB714620BF28" modified="2017-01-18" version="1">MVVNNPNNWHWVNKNCLPWSKDYFEENLQDLELDNEEYLITITSTAVSGDCDVTQRKGKVLCIYDMVLTFDIEGKDKAKESEFKGTIKIPEFIHDETDYDYQLSNFGDHKAHVRKTFVPLIHAKLLKFQSDLIDSHSKDVQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVK1</accession>
    <name>A0A1E3NVK1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Amine oxidase</fullName>
            <ecNumber evidence="15">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">WICANDRAFT_97685</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a primary methyl amine + O2 + H2O = an aldehyde + H2O2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16153"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:228804"/>
            <dbReference type="EC" id="1.4.3.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <cofactor evidence="15">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="15">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <text evidence="14">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="14 15">Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the copper/topaquinone oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="15"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036375.1">
        <property type="nucleotide sequence ID" value="XM_019186606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVK1"/>
    <dbReference type="STRING" id="683960.A0A1E3NVK1"/>
    <dbReference type="GeneID" id="30203852"/>
    <dbReference type="OrthoDB" id="5379943at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009308">
        <property type="term" value="P:amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.20:FF:000001">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.40:FF:000014">
        <property type="entry name" value="Peroxisomal primary amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.20">
        <property type="entry name" value="Copper amine oxidase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049947">
        <property type="entry name" value="Cu_Am_Ox_Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049948">
        <property type="entry name" value="Cu_Am_ox_TPQ-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000269">
        <property type="entry name" value="Cu_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015798">
        <property type="entry name" value="Cu_amine_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036460">
        <property type="entry name" value="Cu_amine_oxidase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016182">
        <property type="entry name" value="Cu_amine_oxidase_N-reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015800">
        <property type="entry name" value="Cu_amine_oxidase_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015802">
        <property type="entry name" value="Cu_amine_oxidase_N3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638">
        <property type="entry name" value="COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638:SF86">
        <property type="entry name" value="COPPER AMINE OXIDASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01179">
        <property type="entry name" value="Cu_amine_oxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02727">
        <property type="entry name" value="Cu_amine_oxidN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02728">
        <property type="entry name" value="Cu_amine_oxidN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49998">
        <property type="entry name" value="Amine oxidase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54416">
        <property type="entry name" value="Amine oxidase N-terminal region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01164">
        <property type="entry name" value="COPPER_AMINE_OXID_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01165">
        <property type="entry name" value="COPPER_AMINE_OXID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13 15" id="KW-0801">TPQ</keyword>
    <feature type="domain" description="Copper amine oxidase N2-terminal" evidence="18">
        <location>
            <begin position="23"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase N3-terminal" evidence="19">
        <location>
            <begin position="115"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase catalytic" evidence="17">
        <location>
            <begin position="242"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="319"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via topaquinone" evidence="13">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <feature type="modified residue" description="2',4',5'-topaquinone" evidence="14">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="20">
        <location>
            <position position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ57168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="633" mass="71047" checksum="211DC18F7D00D270" modified="2017-01-18" version="1" fragment="single">MERLQQIASQTTASAAPPPAPAHPLDPLSIAEIKAVVSVVKAKYGSGAEISFNTVTLKEPSKKSFLEWKDKNGPAPPRFAFVVLLEAGKPGVQEIVVDITNLYVTNLKHVHGVQPILTVEDLQSTEDVIRKDAGVIEQCILSGIPATDMHKVYCDPWTIGYDERWGNSRRLQQAMMYYRSDEDDSQYSHPLDFVPIVDTETKEVIYIDIPNVRRPVSKHPHSNFYPSDAVKEHGYRTEQKPINVTQPEGVSFKMDGHVMEWSNFKFHIGFNYREGIVLSDINYNDHGNVRPIFHRMSLAEMIVPYGSPDFRHFRKHALDIGEYGAGYMTNPLALGCDCKGVIHYLDAHFADRAGDPITIKNAVCIHEEDDGILYKHSDFRDDFATTIVTRATRLVISQIFTAANYEYCIYWNFLQDGSIRLDVKLTGILNTYVANEGEKVGPWGTQVYPQVNAHNHQHMFSLRIDPRIDGDNNSVGSSDVMSSPHELGSLENPYGNGWLGKRTVYKTVSDSLTHANAETSRSWDIFNPNVLNKYSGLPVSYKIVSNQTPRLLAKENSIPSKRAPWARHAMNVVKYEEDRLYPSGVHVPQWSGDGPVGIRGWIGDGSDNIENTDVIVFHTFGISHVPAPEDFPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVY7</accession>
    <name>A0A1E3NVY7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">proteasome endopeptidase complex</fullName>
            <ecNumber evidence="4">3.4.25.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_81512</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="4"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036503.1">
        <property type="nucleotide sequence ID" value="XM_019185245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVY7"/>
    <dbReference type="STRING" id="683960.A0A1E3NVY7"/>
    <dbReference type="GeneID" id="30202491"/>
    <dbReference type="OrthoDB" id="37597at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080129">
        <property type="term" value="P:proteasome core complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03761">
        <property type="entry name" value="proteasome_beta_type_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000013">
        <property type="entry name" value="Proteasome subunit beta type-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF3">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="active site" description="Nucleophile" evidence="13">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ57296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="285" mass="31202" checksum="6CB0B738E2890923" modified="2017-01-18" version="1">MDAIASKYTHKISSLSALGYDDEDALKHDFVNNVNLPLQPSLSMPPISQPTDFLRAHTDDSQNPDCKIKIAHGTTTLAFRFQGGIVVAVDSRATAGNWIASQTVKKVIEINPFLLGTMAGGAADCQYWETWLGTQCRLHELREKSRISVAAASKILSNLVYQYKGMGLSMGTMVCGYTKKEGPTIYYVDSDGTRLKGDVFCVGSGQTFAYGVLDSNLKWDLTVEEALYLGKRSILAAAHRDAFSGGSINLYHITEDGWVFHGNHDVGPTFYEIKEQEGSFNNVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWM7</accession>
    <name>A0A1E3NWM7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_102067</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="protein sequence ID" value="ODQ56967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_019180343.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3NWM7"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:cysteine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF63">
        <property type="entry name" value="HIGH AFFINITY CYSTEINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="369"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="430"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="463"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ56967.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="59956" checksum="A403D640FF7BB99D" modified="2017-01-18" version="1">MSPIKKEADSINVQELKNGDGASSTEFKQTDLADGEKVTYKEADELLKFMEQYEPTSKELTPEESKHLDKKNFSITLLLVTLVTLILFMDKATIGYTTILGIYKDTDLTKKGFNNLNSIFYAGYLIAQWPCHILMQKFPIGKYLSISIFLWAVILGGTAACKNYSQLMACRFLLGATESVVTPACEITLGMFLDTEQREVAQPIFWMATAIAPLISSFVAYGLLHVENVSTFPWRIFMSLNAGLSLILALIVWIFYPDNPSNAKFLTVDEKVHLIKKIQNSTKSSIEQKTIKKHQIIETFKDPISWLFALQSFTLMLSNSLHYQQNQLYLDIGVNNLGSSLVSATGAGFDILMYLVFTIIIKKFPNQNGYIGSICLIIPIASSIAMVTIPWENKKALLANMILAGSTKGVAYIVELGWTTSSAAGYTKKLYRNVMFMIAYSVANMISPQLWNPKDAPRYYSSWIVQIVVAFFLNGVILLIIRLILSKRNKQRLQDDDDKDYYVQTDEGVLERVDVAMLDLTDLENKKFIYPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWT0</accession>
    <name>A0A1E3NWT0_WICAA</name>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
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        <name evidence="2" type="ORF">WICANDRAFT_64930</name>
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    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133560" evidence="1">
        <location>
            <begin position="25"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="167" mass="19511" checksum="C3ECDDC4E5C02751" modified="2017-01-18" version="1">MGSSRLSCARAFLILVFRAAAVHSRAKEQEIQDFYIYAKSRLSEFSKELNDSDSEIYETFDDDDDEDDDYEAYFHNLLYIIQKDVFKKAEKLCSEMSIEHSCSYGMELTKRQIAMKCIENELPFDVGLYEEFLNHGFTLNKTSTTIEPYFSTISDLIVELCKEKYMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX24</accession>
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    <protein>
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            <fullName evidence="3">Gluconeogenesis factor</fullName>
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    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
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                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043743">
        <property type="term" value="F:LPPG:FO 2-phospho-L-lactate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="CofD-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MATERNAL EFFECT EMBRYO ARREST 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31240:SF0">
        <property type="entry name" value="MATERNAL EFFECT EMBRYO ARREST 18"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="419" mass="46938" checksum="8B2B96B41A6F1220" modified="2017-01-18" version="1">MNNQLVVISGGTASNSLVNLFQSLSPNITYILPISDNGGSTSEILRVLGGPAIGDLRSRITRLIPDEGFKNLLSYRLSEDGDEAKLEWTSIVEGQHEIWNGLEIQCKEMVRSFLIHVHMELLKKSRNPSTNFKYEMASIGNLLLTGIRLFVGSLDSAIELILRMTRISPNIKILPCLNTNYTYHISAKLHNGDIITGQSQISHPNEPISTILSSSTSIHKIYDPVIIHEDIPYQHPDLNISQLHFSKNENQPLKSPIDKIFYINPYGEEIHPQANSRTIHSIHDSQTLIYSVGSLMTSTIPVIILKGIGMAIKKSSANKILLLNGDYDRETFRLSALNFIETLCGAVKYSIDDDRVLLTYDEVVTHLIYMEDSEIPVDVEKISYLGIKCISVPKDKKLNENINVYDLSSLQNSLKTLIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXP1</accession>
    <name>A0A1E3NXP1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ACT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_34501</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetolactate synthase small subunit family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ57959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019182194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXP1"/>
    <dbReference type="STRING" id="683960.A0A1E3NXP1"/>
    <dbReference type="GeneID" id="30199440"/>
    <dbReference type="OrthoDB" id="2013116at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
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        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990610">
        <property type="term" value="F:acetolactate synthase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04878">
        <property type="entry name" value="ACT_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000001">
        <property type="entry name" value="Acetolactate synthase, small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="entry name" value="ACT-like. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004789">
        <property type="entry name" value="Acetalactate_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019455">
        <property type="entry name" value="Acetolactate_synth_ssu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039557">
        <property type="entry name" value="AHAS_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054480">
        <property type="entry name" value="AHAS_small-like_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053050">
        <property type="entry name" value="ALS_regulatory_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00119">
        <property type="entry name" value="acolac_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31242">
        <property type="entry name" value="ACETOLACTATE SYNTHASE SMALL SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31242:SF2">
        <property type="entry name" value="ACETOLACTATE SYNTHASE SMALL SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22629">
        <property type="entry name" value="ACT_AHAS_ss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10369">
        <property type="entry name" value="ALS_ss_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="7">
        <location>
            <begin position="76"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="271"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="297" mass="32570" checksum="AF3A12C64AAB9C7C" modified="2017-01-18" version="1">MVSPILKSSLNQRLIRQLGQRSNSSSTSALAYKLLRNKQRPPLPTLETPKWSAESAVASILYESPSDVKPPVKQHVLSCLVQNEPGVLSLVSGTLAARGFNIDSLVVSNTEVKDLSRMTICLREKDAVIEQARRQIEGLVPVYAVLDYTNAEIIKRELLLARVSLLGPEYFEDLLEHHGDLTGSQDSNASGSKYHPRNLTVSEILRLKNEYFSAIQKIVDQFGGKVVDISDRNIIVELSAKPSRISSFLTLIRPFGVLEVARSGMMALPRTPLSDSTEDAPAVNADDVVDVSQLPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXV8</accession>
    <name>A0A1E3NXV8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hap4 transcription factor heteromerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_64147</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037205.1">
        <property type="nucleotide sequence ID" value="XM_019183675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXV8"/>
    <dbReference type="STRING" id="683960.A0A1E3NXV8"/>
    <dbReference type="GeneID" id="30200921"/>
    <dbReference type="OrthoDB" id="3981308at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018287">
        <property type="entry name" value="Hap4_TF_heteromerisation"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10297">
        <property type="entry name" value="Hap4_Hap_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hap4 transcription factor heteromerisation" evidence="4">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="35"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="49"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="275" mass="31157" checksum="73F26A44789ED1F6" modified="2017-01-18" version="1">MTHKYKHTNRLERNYPHIDINHLQSINKPTIIKTSKNWVLPPRPRPGRKPTTQSNTQSQSSTSSTTTTFQPQQAQPQPQQPKKTKKKDVSIQNALKKIQDENQSLKLELSKLVSDLKTLQSETSQFETSNSSSSSSPNSLHKKRNHLEMEDELTSYKSLSLFDESDDDNSSQISTPSLMSNSSSSIGSTLSSVPEDDVLKIEDFLNLSYFENSTNGTISIPPTSQPSKKPIMGFKKNEQHENIEFQFLKDQGVFKGDKDLFLEVGNNIEQEIIEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY98</accession>
    <name>A0A1E3NY98_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_95294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037241.1">
        <property type="nucleotide sequence ID" value="XM_019186489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY98"/>
    <dbReference type="STRING" id="683960.A0A1E3NY98"/>
    <dbReference type="GeneID" id="30203735"/>
    <dbReference type="OrthoDB" id="10254604at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15020">
        <property type="entry name" value="FLAVIN REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15020:SF50">
        <property type="entry name" value="UPF0659 PROTEIN YMR090W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="1">
        <location>
            <begin position="8"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="160" mass="17535" checksum="0116475283E05437" modified="2017-01-18" version="1" fragment="single">MSKVAVIGATGKTGSLVVQSLTNAGFDVTGLVRNPAKARTIEQFANINFETFPLESTSVSKIALFLKDFDSVVFAAGVADLSKHTDVIQIELDGAMKIIEACEQAGVKRVVFISSIAASDRDFWYDNDYTRVYYTAKRTIDKVLERSMLDYTIVEPGPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYD4</accession>
    <name>A0A1E3NYD4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Glucose-6-phosphate isomerase</fullName>
            <ecNumber evidence="4 11">5.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_35058</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the GPI family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="4 11"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036794.1">
        <property type="nucleotide sequence ID" value="XM_019182266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYD4"/>
    <dbReference type="STRING" id="683960.A0A1E3NYD4"/>
    <dbReference type="GeneID" id="30199512"/>
    <dbReference type="OrthoDB" id="5831190at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047938">
        <property type="term" value="F:glucose-6-phosphate 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05016">
        <property type="entry name" value="SIS_PGI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1390.10:FF:000001">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000004">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00473">
        <property type="entry name" value="G6P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023096">
        <property type="entry name" value="G6P_Isomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001211">
        <property type="entry name" value="PRK00179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469:SF1">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="7 11" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ57587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="556" mass="62234" checksum="2512924826A05253" modified="2017-01-18" version="1">MASSNTFSDFKLTTDLKAYKDLQELYDNGGKDLKIANQFVKDPKRFEKYSKEFTNFDGSKILFDFSKNIFNDEIFEKLVELAKEAGVEKLRDELFKGEHINFTEDRAVFHIALRNRSNKPLYVDGENVAPEVDSVLQHIKEFSNQVRSGEWKGYTGKKITDIVNIGIGGSDLGPVMVTEALKHYSSPDLNAYFVSNIDGTHIAETLKRVNPETTLFLIASKTFTTAETITNANSAKHWFLEHAKDTAHIAKHFVALSTNAEEVAKFGIDTKNMFGFENWVGGRYSVWSAIGTSVALYIGYENFENFLKGAEAVDKHFTETSIEDNIPLIGGLLSVWYNNFFDAQTHLVAPFDQYLHRFPAYLQQLSMESNGKSVTRGNVFTNYSTGSILFGEPATNAQHSFFQLVHQGTKVIPSDFILAAQSHNPIEDNLHQRMLASNYFAQAEALLTGKNEDQVKAEGATGGLVPHKVFTGNRPTTSILAQKITPATLGALIAYYEHLTFTEGAIWNINSFDQWGVELGKVLAKVIGKELDDKKPVETHDPSTKGLINKFKEWSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYR4</accession>
    <name>A0A1E3NYR4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycine cleavage system P protein</fullName>
            <ecNumber evidence="7">1.4.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_85326</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 7">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GcvP family.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="7"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037462.1">
        <property type="nucleotide sequence ID" value="XM_019185877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYR4"/>
    <dbReference type="STRING" id="683960.A0A1E3NYR4"/>
    <dbReference type="GeneID" id="30203123"/>
    <dbReference type="OrthoDB" id="6537869at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016594">
        <property type="term" value="F:glycine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000005">
        <property type="entry name" value="Glycine dehydrogenase (decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000007">
        <property type="entry name" value="glycine dehydrogenase (Decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003437">
        <property type="entry name" value="GcvP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049316">
        <property type="entry name" value="GDC-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049315">
        <property type="entry name" value="GDC-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00461">
        <property type="entry name" value="gcvP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003346">
        <property type="entry name" value="PRK04366.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773:SF1">
        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773">
        <property type="entry name" value="GLYCINE DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21478">
        <property type="entry name" value="GcvP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="8">
        <location>
            <begin position="43"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="8">
        <location>
            <begin position="649"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="818"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR603437-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1000" mass="110903" checksum="141EC2DEDFBA87D7" modified="2017-01-18" version="1">MLSNPLQRSLTCDISSSNFQKIYDPSISVNAQDRLKPLDNFQRRHLGPNYNDVTHMLETLGYNDLDEFIKHTIPENVLVKRKLNIEPENGFTETEMIENLESIAKKNKVYRSFIGKGYYNTITPPVIKRNVLESPEWYTSYTPYQPEVSQGRLESLLNFQTVISDLTGLPVSNASLLDEGTAAAEALSISFNNSRKKKTKYVIDKDIHPQTLSVLKTRAYPLKIELVQLDLNSKEGLQQLESIKNEISGALLQYPTSSGDIFDYEHLQKIADVVHSSKGLLAVASDLLALTLLKPPSSFGADIAFGSSQRFGVPMGFGGPHAAFFSVIEKLNRKIPGRIVGVSKDRLGNQALRLALQTREQHIKRDKATSNICTAQALLANISANYAVYHGPVRLQKIAKRVYGFTSILANFIRENSSHEIENGQWFDTLTIKLNGVSADEILQKAVDQYQINLFKADDYTVSLSLDESVVTKDLVDLTELFTGKQIPIESLNEFPDFPSSLTRTDKILSNPVFNLHHSETAMLRYLHNLHNKDISLANTMIPLGSCTMKLNATVEMLPLSWPEFGSIHPFVPKDQVKGYKELIEILEKDLANITGFTATTLQPNSGAQGEFTGLRVIKQFHESKGEFDRDICLIPVSAHGTNPASAAMSNFKVIPIKCLENGSLDLVDLEEKVEKHKANLGAIMITYPSTYGLFEPTVRRAIDLVHEHGGQVYMDGANMNAQVGLTSPGDLGADVCHLNLHKTFAIPHGGGGPGVGPICVAEHLVPFLPKHSVFKLRTETETSIESVSSAPYGSASILPISYAYIKLLGSTGLPFSSSIAMLNANYMMNRLKDHYEIAFIGLDGIERCGHEFILDLRKYKDQGIEAIDVAKRLQDYGFHAPTLAFPIPSTLMIEPTESENLEELDRFVDSLISIKGEIDAFLKGEPLGQVLKNAPHSLSDVVSSNDWESRGYTREQAAYPLEHLKTNKFWPTVARLDDTYGDLNLLCTCPSVEEVANQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYS7</accession>
    <name>A0A1E3NYS7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_80516</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037578.1">
        <property type="nucleotide sequence ID" value="XM_019185078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYS7"/>
    <dbReference type="STRING" id="683960.A0A1E3NYS7"/>
    <dbReference type="GeneID" id="30202324"/>
    <dbReference type="OrthoDB" id="61900at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L27a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_uL15_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="6">
        <location>
            <begin position="28"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="105" mass="11762" checksum="ACEE23E0EBBE91FC" modified="2017-01-18" version="1">MDKYHPGYFGKVGQRYFHKQQLHFWKPVLNLDKLWTLVPEDKKEQYLSSSSASAAPVIDTLAHGYGKVLGKGRLPEVPVIVKARFVSKLAEEKIRAVGGVVELVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYV9</accession>
    <name>A0A1E3NYV9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GP-PDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_13227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037475.1">
        <property type="nucleotide sequence ID" value="XM_019180564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYV9"/>
    <dbReference type="STRING" id="683960.A0A1E3NYV9"/>
    <dbReference type="GeneID" id="30197810"/>
    <dbReference type="OrthoDB" id="1058301at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034479">
        <property type="term" value="F:phosphatidylglycerol phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034478">
        <property type="term" value="P:phosphatidylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43805">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43805:SF1">
        <property type="entry name" value="GP-PDE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="1">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="237" mass="26964" checksum="36B001C677BC1A09" modified="2017-01-18" version="1" fragment="single">YKDKYPENTLIGFKGAVDAGVDVLETDVQVTKDGVVVINHDPTTGRVYDKDLVVADSNLDELKDLTNKSDPSQKFLTFQELAEWVITQKDGLKIMLDIKPYNNKLILVKIIRDLKNVYNDLTFWYDKIIFGLWRLDFYEYGVVTGLIKNFEIINISISPVISNTLIEYSQTVPKGFKLNAISILDVSSWANDFDNYRETVLKPNNIGLYLWTVNSPDDIKKSLAVKVDGMVTDDPVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ41</accession>
    <name>A0A1E3NZ41_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_70362</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037646.1">
        <property type="nucleotide sequence ID" value="XM_019184202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ41"/>
    <dbReference type="GeneID" id="30201448"/>
    <dbReference type="OrthoDB" id="3981031at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="120"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="602" mass="69247" checksum="FB47D6AB5DF0D105" modified="2017-01-18" version="1">MTFLSQICMRGSLRSSHTIRQNIRSNNNTRLFIENRFFHSQRNVRPSLQNKALKSPTGGNLFWHYLNAPGNMLFLTTNFIGLGILVTYRCFSSVAVANRTSQDVETNILLDNTMNGLINFDSSGDRFNLLKQQIEELKQEELKLSALEEEYEEEDILETELKELEQEEEDLALMTSRSQLNEEEVDEMLPYTEMELDEYGPPLVRDAKDERFKSLLISQLLSSFYVNDFLGQIFRENEPDLQLLETLSTPAKESLVLALYGLLERQNDGECANVDSFKAFFDKLPVKEIKHFIEDAFASDLRYQGTAYNNTLIKDEYLENSLEAADLESLSMEKINKIIRKDYEFDCEAYLNLAVLLINTKSLAKSPTYDVCAKIVEQYNEKGNNRKHRNLSNITNVLNSVFFHLDQLKIEKLGDPFTQLMKSSIRLGNKTGFHSALRKMKCDEASLLHKQKHNNIKTVTPRDIKFGDKFSIKAYATALEGLLHFRASSEHIHGVIGKLLKSLVSSDGELKVLTQDKVNIDSHSLFSSIPLEVFKLFVRFAEEYSDNVFLGWCFKFMNRYYDVSSTVYDKSHELLRETAISMGNKDLLAQIDDAYKAGRRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ78</accession>
    <name>A0A1E3NZ78_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_94973</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037124.1">
        <property type="nucleotide sequence ID" value="XM_019186476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ78"/>
    <dbReference type="GeneID" id="30203722"/>
    <dbReference type="OrthoDB" id="10627793at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="129" mass="14299" checksum="2409761714953C11" modified="2017-01-18" version="1">MTKNHHHSSPPITYRLKVLHIRHTHLSKLEAQYPDCDELTSNISYPIFNKPTKSRPFSVFSSSGVMSNNNSILNNHSLISDTNSTITGSSTLASSSMNHPADSLTSSKNKIQLKYRGLMTTNEEILPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P000</accession>
    <name>A0A1E3P000_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PhoD-like phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_84482</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037940.1">
        <property type="nucleotide sequence ID" value="XM_019185715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P000"/>
    <dbReference type="STRING" id="683960.A0A1E3P000"/>
    <dbReference type="GeneID" id="30202961"/>
    <dbReference type="OrthoDB" id="2419400at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07389">
        <property type="entry name" value="MPP_PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.70">
        <property type="entry name" value="PhoD-like phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018946">
        <property type="entry name" value="PhoD-like_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038607">
        <property type="entry name" value="PhoD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043904">
        <property type="entry name" value="PhoD_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46689">
        <property type="entry name" value="MEMBRANE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46689:SF1">
        <property type="entry name" value="PHOD-LIKE PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19050">
        <property type="entry name" value="PhoD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoD-like phosphatase" evidence="1">
        <location>
            <begin position="134"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="PhoD-like phosphatase" evidence="1">
        <location>
            <begin position="425"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="680" mass="77405" checksum="3410B0E25E039F9E" modified="2017-01-18" version="1">MSLQDWIQDVQPISKIYEILEASKNVPDECPVNDPLGGVDIRCGPILRLIGTLEDNTDNYRASIMLVVKGSEIPKVSYVQGPVIPDSLDELAKGEYKHQVFSTVGDYKFYRFLIDTKLIAKEQKIRYTVNDVLSYEFFLPAASQPMNIVSYSCNGFSLSTQTETYKSSLWYDVLNRHHSSQRYHVMLGGGDQIYCDAIKNASKVFHDWLEEKNPVKKHHQKLTKEISDSFHDFYINQYMKWYGQGFWKGTNGKILEALFPVAMATIPSINIYDDHDIIDGYGSYRDSTMSTEIFKGVGNIAYQYYMLFQQQVNYKDDDEAYLKDPSWILGSKPGPYMGEKSHSIFTKLGKGIGFLGLDCRTERKLKTIVTKETYELVFKRLQAEVKKDPSIKHLLVLLGVPIAYPRLVWLEWLLNSVILAPARTLAQKGIIAKGLVNEFDGSVEVLDDLNDHWCSKHHKKERNNLIGKLQEFGSKNGVRVTILSGDVHLAAVGRFKTKMHLNHFFNKEKYQEHNDAVKSKPEEDPRLMFNVISSAIVNAPPPDAMAGLLNKRSGIHHFDHNTDEDMVPIFTKDVDGSIRSNKEFLNKRNWADLISIHNIQDKEGYKVGDERLPGPVTGIPEGLKIRDERDLSYRISENSLIATIHVEKNHEDIKSETLGYEIAIPGLSGKYQLENIGLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P024</accession>
    <name>A0A1E3P024_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_84536</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peptidase M18 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038018.1">
        <property type="nucleotide sequence ID" value="XM_019185725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P024"/>
    <dbReference type="STRING" id="683960.A0A1E3P024"/>
    <dbReference type="GeneID" id="30202971"/>
    <dbReference type="OrthoDB" id="9880441at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05658">
        <property type="entry name" value="M18_DAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.250.10:FF:000001">
        <property type="entry name" value="Aspartyl aminopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.250.10">
        <property type="entry name" value="Aminopeptidase i, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001948">
        <property type="entry name" value="Peptidase_M18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023358">
        <property type="entry name" value="Peptidase_M18_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570:SF4">
        <property type="entry name" value="VACUOLAR AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02127">
        <property type="entry name" value="Peptidase_M18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00932">
        <property type="entry name" value="AMINO1PTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101821">
        <property type="entry name" value="Aminopeptidase/glucanase lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="25"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="537" mass="58544" checksum="B600088D0333B563" modified="2017-01-18" version="1">MAYSQKDLEKAIYKTTMLTLKDVLSRQTSESKSSKSKLPSNTSDKKSKRRSTNVPSFNVEKVTYDDDYFIKYADEYITFTGKNPTTFHAIHFFSKLLEANGFKYLSEKDEWSDLSAGLYYTTRNSTSLGAFAVGKDWKPENGVGIVGSHIDALAAKLKPVSSKTKVDGYELLGVANYAGALSPTWFDRDLGIGGRVLVRVEDKNSSTGYKIISKLISSSPKPIARISTLAEHFGAAAAPPYDKETKAIPVIGYNSGSDSEPTKEEKKSPLIGKHPIDLLRFIAELAETSVADIVQLDLDLFDVQPGTKGGLRDDFVFAPRIDDRVCSFAAINALIEFTKNGPIPSDSFSQVLLFDNEEVGSLTRQGAKSTLVNSITERVIDSLVGSKDVSVEVASRTAFANSVILSADVTHLLNPTYKSEYLDDHFAVPNKGITIALDPNGHMATDSVGQTIAQEVARLNDDELQYFQIKNGARSGGTIGPTISSNTGARTIDLGIPQLSMHSIRAAIGVKDVGLGVKYFNGFFKYWQDVNKSFGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P057</accession>
    <name>A0A1E3P057_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2',3'-cyclic-nucleotide 3'-phosphodiesterase</fullName>
            <ecNumber evidence="3">3.1.4.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_31783</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the metabolism of ADP-ribose 1',2'-cyclic phosphate which is produced as a consequence of tRNA splicing.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 2H phosphoesterase superfamily. CPD1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.37" evidence="3"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037942.1">
        <property type="nucleotide sequence ID" value="XM_019181880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P057"/>
    <dbReference type="STRING" id="683960.A0A1E3P057"/>
    <dbReference type="GeneID" id="30199126"/>
    <dbReference type="OrthoDB" id="514292at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009187">
        <property type="term" value="P:cyclic nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012386">
        <property type="entry name" value="Cyclic-nucl_3Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28141">
        <property type="entry name" value="2',3'-CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28141:SF1">
        <property type="entry name" value="2',3'-CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07823">
        <property type="entry name" value="CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ58735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="212" mass="24013" checksum="7F1AC8B7487506E8" modified="2017-01-18" version="1" fragment="single">LAIALWLCPKQSSQIYETLNSLIIGINPLFEDAPRFEPHITITSQLSINSMSDVNTVLNSCVAALAGGPEMDIELTFNSLKINHKNSYFKKIYLSLLKTKNLASLATIIRELYVEFPNLQNQAESKYAAQDWCNEIFDPHLSLVYTNLTHFDNALIRTIKTRVEDLLNTDTLSLEDDELLEYNLGWTNGALKIVRCEGPVEEWQVLGKVDIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P099</accession>
    <name>A0A1E3P099_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_79437</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038098.1">
        <property type="nucleotide sequence ID" value="XM_019184893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P099"/>
    <dbReference type="GeneID" id="30202139"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="510" mass="58616" checksum="4D967AFFAEAF6B39" modified="2017-01-18" version="1">MSDSSDAEQTHVIGGDALHSIRLSGFLQDNRDQLLLNIKREFTKPLMEINYQLLLGNFHESRLNGTNQESLLSEVSAGGFSISELLVELCKFIELEIENTDPRLKDEKSIRGITQIIAGDFGEEVYYNLWKFWFLVPGSSNVVDFDDLGGSTDLVGSSINKVTNNLGKMKFIPSKIDPELIFLPVIEKLETKIKRILKQKANPFSYVEVLTDLLSSIKKSLKGEYRVAKKKEFDLKSEHNNRVVSPLLDFINYLFGTKLASTGKELSFDYQNLQNTTTSRLVKKRLKALESESKITLYPDITLLDENEIQNGYDSNMRYLLLEYKTPRILIDLNSPEHFNKTVINKSLIQTYSYALCIAGQKSGTIIDFETSLFIKFPKRFTEEPQNNRRRRRQNNNNNNDGDDQSNGKRKLIANIQYYIVDDSQLYNQSEEPTFDQKFNSKILLATEIYNQFLEDEEEPKKSDQKRRKLKASSLISDTASLEHLDETKLEFNKIDALLSRTRSELVESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0C5</accession>
    <name>A0A1E3P0C5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inheritance of peroxisomes protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_64453</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for peroxisome inheritance.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the INP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037524.1">
        <property type="nucleotide sequence ID" value="XM_019183739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0C5"/>
    <dbReference type="STRING" id="683960.A0A1E3P0C5"/>
    <dbReference type="GeneID" id="30200985"/>
    <dbReference type="OrthoDB" id="3980558at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005780">
        <property type="term" value="C:extrinsic component of intraperoxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045033">
        <property type="term" value="P:peroxisome inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024758">
        <property type="entry name" value="Inp1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12634">
        <property type="entry name" value="Inp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="56"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="67"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="469" mass="53230" checksum="B6DEF56D0A3026BF" modified="2017-01-18" version="1">MLTSSSPMQALSPTTSLTGKSMTSDEIVDITNSRKLLRPTNEFGSLVLTPRKRALARKNNNNNNNNDPITSTPASSNGMVSPPLSSQKSIETLNSPQKALEDPIKINSVHEEETFDPEERITLFHFFKANIYSFEEMDNMEEKQGRLLGHGKFEVYQMHMKKVSYFQCGSVVYPILPRLKILKISHNQFILPLSNPERYWRIVIETNDSLIIKELEEVYKKICHFRNLYIQSTSELPITKTLPPPITTLPTSKSQASLSSITTAVACFALESDSSTFVEQISTESTPLPTQDEEETDEVESVVSSLDSALEDLTEDPKHFIQQEQLETSRYFTPSSSVQHTTLIDESRDTILLSPTFQHESSRILPKSRSSRSASLYIAESSWMDPTDDLITSSPGTDRYIKLNLNPNPRFLNKKKEKNLQKRIVTDSVLTNKSHRYSSYDVYNMLCDEDLDHQEKDAGFTGFLKSFWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0P6</accession>
    <name>A0A1E3P0P6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_79385</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038047.1">
        <property type="nucleotide sequence ID" value="XM_019184880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0P6"/>
    <dbReference type="STRING" id="683960.A0A1E3P0P6"/>
    <dbReference type="GeneID" id="30202126"/>
    <dbReference type="OrthoDB" id="10253390at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021829">
        <property type="entry name" value="DUF3419"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47473">
        <property type="entry name" value="BTA1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47473:SF1">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11899">
        <property type="entry name" value="DUF3419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransferase" evidence="2">
        <location>
            <begin position="136"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="790" mass="89900" checksum="CC063396491E96EF" modified="2017-01-18" version="1">MSFKNIILDNSVYYKDTVQSHLFNSAHYLSDSTYAQYLKFAAVVLTGCSFYLYFLANEKTRSFLVFIWACFVKPFTPSNRKVHNDHQNNLELFYKTQAKFYDTTREVLLQGRENAMKLAFSHLRADHKTRKLVWIDIGGGTGLNIEKMDQYVAKLNDNFEAVYLVDLSPSLCDVAKGRVAKHGWKNVHVICGDACDFEIPHQEVDLITYSYSLSMIPLYHSAIDHAAKFLKPDEGIICSVDFGVQSDSTSIGRTNTLGGLVNRHVPWFYRTFWRIWFECDKVFLDPSRREYLDYKFGTLKSTNCYNSTLGQIPYYIWLGVNKQHDSQLMYRFNSLATESPYLAPIDPKKSGAINKDAPTSKALEAALDNVKKGLPYPSLFYQKQTWRVYYDELSDDYECFKNQYIYAFTWEDPREDAKILNLTSKDTVLAITSAGDNILSYASLGSNAPKRIHGVDLNPCQGHLVELKLAALTALSHEETWKLFGEGKIENFQELLIGKLAPHLSSNAFQYWMEKGPKTFSITGKGLYDTGSTRWALRLARYVFKITGLSSKVEELCNAKTLNEQKEIWNTSIRPVLYSAIVGKIFVGNPVFLWKALGVPANQAAMMDASILQYIIDTLDPIIGRSLVSSDNYFYYLTLKGHYTPKNCPDYLTKSGFKQLTKTSNSPLEGIRLHTDYLNNVLARLTKKTISVAVIMDHMDWFDPEGKEVDDEIDALHSALCDGGRVMLRTAAQVPWYIKHFEAKGFSCTAAATRYSGTSIDRVNMYASTWICTKIPTKKERQMSTLTLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P133</accession>
    <name>A0A1E3P133_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_33288</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038322.1">
        <property type="nucleotide sequence ID" value="XM_019182054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P133"/>
    <dbReference type="STRING" id="683960.A0A1E3P133"/>
    <dbReference type="GeneID" id="30199300"/>
    <dbReference type="OrthoDB" id="5121955at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052073">
        <property type="entry name" value="Amide_Lactam_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171">
        <property type="entry name" value="FARA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171:SF3">
        <property type="entry name" value="FARA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="41"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="81"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="92"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="603" mass="68772" checksum="C63C9F2EF69C736D" modified="2017-01-18" version="1" fragment="single">MKQEENHFVVPNTQEDPSADDKPKDTKKYSVKFKRSRSSRACEVCHSRKVRCDAMIHIPCTNCLTFGCECKFPEPKIRKNAKKKKVENNDISTTENSNHTTTTSSNGLEKPVLKQDPPTISHSKKKNEPVAAVSAQSAKIDQSKFSQKMSSISFIGTSSTANLLTDRANVSSSHLIKDEFVPPTLNRIMEKTRIGLDTVQMEILRVRGAFLLPEKQLCDDLVNTYFERIYPVEPLVDKEKFIKAYKEGSISILLLQSVLLAASRVSENPSLYDSDGSNYLASCTFFQRAKALYDANYERDPLTLVQSLTLFTRFWEGLDDILGNSYYWSRVAVTVAQGYGFHRTLNDTILTPEEMKKWKLAWWNLYIKDISASVSFGRPKIIHLEDCDVEMLRLEDFPDDVSYEDAESFIQLIKLSEIISIVMQEQYSAKAERFKHRENWVITHCDMLMSSWRNNLPHNLQYVPNKKFPLSVNCLNLYYYSAVCLVHRSNMVRTATLHGKTYPSEGIVFQASRIIADIAAKMMKYDQMKYCNALVVTSLFTASMTFICHMDSSNSSISKTARLGYEVLFKALNEFGKNYLIAAVIAHNMQKLASDNKARQKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1I0</accession>
    <name>A0A1E3P1I0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial dicarboxylate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_84929</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038553.1">
        <property type="nucleotide sequence ID" value="XM_019185809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1I0"/>
    <dbReference type="STRING" id="683960.A0A1E3P1I0"/>
    <dbReference type="GeneID" id="30203055"/>
    <dbReference type="OrthoDB" id="448427at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005310">
        <property type="term" value="F:dicarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000107">
        <property type="entry name" value="Mitochondrial dicarboxylate carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="34"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="122"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="223"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="34202" checksum="453851CCC3AD3967" modified="2017-01-18" version="1">MSKIVQIQHQQQQAECTTNSQPSIPLISKPKEKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMALSIWKHDGILGVYSGLSAGLLRQATYSLTRFGVYEYLKENYVPSDKQSSMAYLLPISMISGGVGGIVGNPSDIVNIRMQNDTGLPLDKRRNYNHAINGVTRIIKEEGVSSLFRGLGTNLVRGVLMTSSQVVTYDISKNLLIKNLQMDKDSKLTFFSASLIAGLVATTICSPADVLKTRIMNSSGSGEGVLKILAHAFKNEGPSFMFRGWVPSFVRLGPNTIITFLVVEQLREYRVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1I4</accession>
    <name>A0A1E3P1I4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">H/ACA ribonucleoprotein complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_32809</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ("psi") residues may serve to stabilize the conformation of rRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the GAR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037979.1">
        <property type="nucleotide sequence ID" value="XM_019182007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1I4"/>
    <dbReference type="STRING" id="683960.A0A1E3P1I4"/>
    <dbReference type="GeneID" id="30199253"/>
    <dbReference type="OrthoDB" id="2187159at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034513">
        <property type="term" value="F:box H/ACA snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000454">
        <property type="term" value="P:snoRNA guided rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.230:FF:000001">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.230">
        <property type="entry name" value="Probable tRNA pseudouridine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038664">
        <property type="entry name" value="Gar1/Naf1_Cbf5-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007504">
        <property type="entry name" value="H/ACA_rnp_Gar1/Naf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23237:SF6">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23237">
        <property type="entry name" value="NUCLEOLAR PROTEIN FAMILY A MEMBER 1 SNORNP PROTEIN GAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04410">
        <property type="entry name" value="Gar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="123"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="141"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="208" mass="21080" checksum="471AF48A4CF8377F" modified="2017-01-18" version="1">MNRGGNRGGFGGRGGRGGFGGRGGFGRPSAPQGPPDSVLEMGAFLHPVEGDIFCRSINTKIPYFNAPIYLENKTQIGKVDEILGPLNEVFFTVKPSEGVQAASFKEGDKFYIAPDKLLPIERFLPKPKVAGPKPSKKKSAGGRGGARGGFGGRGGARGGFGGRGGSRGGFGGSRGGFVGRGGSRGGFGGARGGSRGGFGGRGGRGGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1K5</accession>
    <name>A0A1E3P1K5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_93978</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038281.1">
        <property type="nucleotide sequence ID" value="XM_019186437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1K5"/>
    <dbReference type="GeneID" id="30203683"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="54" mass="6243" checksum="712D5A158FC906E1" modified="2017-01-18" version="1" fragment="single">MEYWNVFLRSDLNKPGKKEKAKDLVLIPGNNQSSNNSRQQKKALVPNRNLSFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1L3</accession>
    <name>A0A1E3P1L3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_63713</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038425.1">
        <property type="nucleotide sequence ID" value="XM_019183596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1L3"/>
    <dbReference type="STRING" id="683960.A0A1E3P1L3"/>
    <dbReference type="GeneID" id="30200842"/>
    <dbReference type="OrthoDB" id="10260596at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2650:FF:000001">
        <property type="entry name" value="40S ribosomal protein S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001377">
        <property type="entry name" value="Ribosomal_eS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014401">
        <property type="entry name" value="Ribosomal_eS6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018282">
        <property type="entry name" value="Ribosomal_eS6_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11502">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01092">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosom_S6_euk"/>
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    </dbReference>
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        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00578">
        <property type="entry name" value="RIBOSOMAL_S6E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ59218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="244" mass="27720" checksum="E5CBEED919F24660" modified="2017-01-18" version="1">MDFKNNKAGRLNIAYPANGTQKTVDIDDEHRLRVFYDKRIGTEVEGESVGDEFKGYVFKITGGNDKQGFPMKQGVLLPNRVKLLLAKGHSCYRPRRSGERKRKSVRGAIVGPDLAVLSLVIVKQGDADIEGLTNEQVPKRLGPKRANHIRKFFGLTKEDDVRQYVIRREVVKGDKKYTKAPKIQRLVTPQALQRKRHLKSLKIRNAQAQRDAAAEYAQLLAQRLHERKAEKAEVKKRRASSLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1M9</accession>
    <name>A0A1E3P1M9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_13923</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MSOX/MTOX family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038445.1">
        <property type="nucleotide sequence ID" value="XM_019180588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1M9"/>
    <dbReference type="STRING" id="683960.A0A1E3P1M9"/>
    <dbReference type="GeneID" id="30197834"/>
    <dbReference type="OrthoDB" id="2219495at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051698">
        <property type="term" value="F:saccharopine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008115">
        <property type="term" value="F:sarcosine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045170">
        <property type="entry name" value="MTOX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961:SF26">
        <property type="entry name" value="L-SACCHAROPINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961">
        <property type="entry name" value="PEROXISOMAL SARCOSINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="390" mass="43868" checksum="FCE8E4D5E92F30E9" modified="2017-01-18" version="1" fragment="single">VLIVGCGVFGLSTALELARKGYKVKAIDLYPIPSKWAASNDLNKIIRTEYADIFYTKLSVEALKLWENDPLYKDVYFKSGRITLSPSSKENQHRTKFENQGFANLAKLGVDVSGIFEIDNGAKLTGLFPQFKGNDFGEISAKFNPNAGYGHASNSLLNVYQEARKLGVEFIFGTKGNAKKIINNNSIQVESGDVYTADKILIAAGAATGFLVDLKDQIRALGLFVTHIQLTPDEYTKFKDIPIFFSAEYGYFFPPDAKHHHMKIALTYSDAENKIPDPFNSDKLLSLPRFKEQNQQDTFPKKGESHVRRLLQKTIPELSQHELYNSKLFWISDSPTNDFLIDKLPDSENIVVACGDAGHGYKFLPNIGKYITLKMQGKLDEITSKRWSWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1P3</accession>
    <name>A0A1E3P1P3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tryptophan synthase beta chain-like PALP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_105935</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038321.1">
        <property type="nucleotide sequence ID" value="XM_019180457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1P3"/>
    <dbReference type="STRING" id="683960.A0A1E3P1P3"/>
    <dbReference type="GeneID" id="30197703"/>
    <dbReference type="OrthoDB" id="10259545at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000016">
        <property type="entry name" value="Cysteine synthase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="7">
        <location>
            <begin position="46"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="383" mass="41954" checksum="651BB7BF9C2ACD34" modified="2017-01-18" version="1">MPQSFTWKEISACGISLIGILYTLNQIYKTSSPKLSKLSPPEIGVEGLIGNTPMILIKSLSKHTGNNIYAKLEYMNPGGSAKDRVALAIIQSNQDNLKKGDRIFEGTSGSTGISIGTVGNSLGYKSEIHLPDDTSPDKLVLFETLGVDIKKVKPASIVDPSQYVNSAKNANEVFNNSGDENNGVFADQFENDANWRIHYKTTGPEIYKQLQGKIDVFIAGCGTGGTIAGVSRYLKEKLSNVVTILADPQGSGFFNRINYGVMYTNEEKEGTRRRHQVDTIVEGIGLNRITHNFSMGEEYIDYSLKIKDEEAIKMAKYLSVNDGLFIGSSTAINAVAAVQACQQNGWKNKNIVIIACDSGTRHLNKFWKEAVKVPNDVKLEDIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1T2</accession>
    <name>A0A1E3P1T2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_69528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038361.1">
        <property type="nucleotide sequence ID" value="XM_019184169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1T2"/>
    <dbReference type="GeneID" id="30201415"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="560"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="569"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="779" mass="88013" checksum="891638852D42F595" modified="2017-01-18" version="1">MSSSQTTKRRRSDIRKQSSSYLYYNYDRETGRAICNECLKVDQKTVIEGFPSGGLTSQLFSHLAVKHDISKESCDQYQAQLKKGEATTGERLQPTLAVSLTGVRLSTPDRKNQNQHGEETSSQERYSTLIQKSLIKNNNPLNFYDDEITQELFSAIPGFKSPSSARVAFDLSQNCARLLNEVAQTLVDDDIPISLSFNEVNIDGNSFVAINSHFIHDATVNEVTLGFIETSFGSASLLKQAVSKTIGLLGLKDQVISITTEMESSSAFARELLIQEKEFPSLSISQMPGCSRQLLNWVASGFIRDFTIQSTANFEHFTKSDIELRRVVTGILSYFSRDEEGNTSTSINDLVSLMNRIALMVNNSPDLKEHYTTIKKNYEAQNKDVYVPDIVEPNEESLSSFLGSIKALTRNKAILVQLSQRDHLNMLPKVSPTEYKVAYCIDLALKDFDTFIDRLTSKKFSMNFVLPIIEEVIMNMKELEQTANATPVLRQGARHGAIKASRYYKRIKNDPLVLLSSLINPQIKDICPNFIFETITEDPQCLNIISEFLMNNGYIKRSRKREMDKVDINDSSSASATPKSPRKPASDLKKVIEKRLKSGLMSSQEYQKIMDVIEKEKSTDGSHSKETFTKSSAKEEVMRFFNFIESAEFYNLPSTFDTNTDYFKNLMKFYLTDTKFKSMFPNIQTAIKICLTAQASSTSSELTSIQARSRWALDGKETSLRIYETDGNEELLNTTLSSFMILKSLCNEDSTLASLEKFNIEASNEQEVSEKYILTLMLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1X1</accession>
    <name>A0A1E3P1X1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Replication factor C subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_84970</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038612.1">
        <property type="nucleotide sequence ID" value="XM_019185816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1X1"/>
    <dbReference type="STRING" id="683960.A0A1E3P1X1"/>
    <dbReference type="GeneID" id="30203062"/>
    <dbReference type="OrthoDB" id="761538at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000030">
        <property type="entry name" value="replication factor C subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000002">
        <property type="entry name" value="Replication factor C subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000136">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF1">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13177">
        <property type="entry name" value="DNA_pol3_delta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22534">
        <property type="entry name" value="RFC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="34"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00070185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="353" mass="39635" checksum="4AFAB8BCE5E86E8E" modified="2017-01-18" version="1">MSLWVDKYRPRTLDSIDYHEDVSKRLKALASSGDFPHLLVYGPSGAGKKTRVIAVLHELFGAGVEKMKIDVRTFQTSSNRKLEFNVVSSPYHMEITPSDLGNNDRVVIQDLLKEIAQTEQVDFSGKHRFKVVIINEADSLSRDAQAALRRTMEKYSKNIRLILISNTTSNIIAPIKSRTLLLRIPSPTIDDITKVLSKLASKQEIQLPSDEQDKIQVLNNVGASSKRNLRRALLLLEALTMQNETLKTTTPMITLDWEGVIKKLAGNISRDRNVARISSSRTVLYELISHCIPPKIILKTLAFELLNFVNDKVKSDIVELAAIFDERLSLGTKSIFHLEGFIAKSMVAIEKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P204</accession>
    <name>A0A1E3P204_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphotransferase</fullName>
            <ecNumber evidence="8">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_79472</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the hexokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="8"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038134.1">
        <property type="nucleotide sequence ID" value="XM_019184899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P204"/>
    <dbReference type="STRING" id="683960.A0A1E3P204"/>
    <dbReference type="GeneID" id="30202145"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019158">
        <property type="term" value="F:mannokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000034">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019807">
        <property type="entry name" value="Hexokinase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF30">
        <property type="entry name" value="GLUCOKINASE-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00378">
        <property type="entry name" value="HEXOKINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="9">
        <location>
            <begin position="10"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="10">
        <location>
            <begin position="227"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="480" mass="52980" checksum="20F95E0A3745E784" modified="2017-01-18" version="1">MSSSKQLKEEVDKIVKDFTVDETLLKTLTDYFIESMDAGLSHKSEEKSKGLPMIPTYVTRIPTGREHGTFLAADLGGTNFRVCSVDLHGDSTFALEQSKAKIPEDLLDGTSEELFTYLAKKVKQFVERHHADKVSTDSEKHLKLGFTFSYPVNQTALDAGTLIRWTKGFKIQDVVGKDVVQALQTELDKQSVPVKVVALANDTVGTLLSHAYATGASPNGKQGDTVIGAIFGTGTNGTATFPLEDIHKLDPELVKELKANGETHMIINTEWGSFDNDLKHLPNTIYDQRIDEVSTNPGYHMFEKRVSGMFMGEILRQVLVELHSKGVMLQQYPTPERLPHRLRTPFELDSEVLSHIEIDDSSHLKETELSLLQSLRLPTIEEERTAIQQIVRAISRRSAYLAAVPIAAILIRTNALEGYHKEVEIGVDGSVIEFYPGFRSMVRHALALSPIGPDGERKVHICIAKDGSGVGAALCASTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P214</accession>
    <name>A0A1E3P214_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VHS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_15786</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038144.1">
        <property type="nucleotide sequence ID" value="XM_019180661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P214"/>
    <dbReference type="STRING" id="683960.A0A1E3P214"/>
    <dbReference type="GeneID" id="30197907"/>
    <dbReference type="OrthoDB" id="10068368at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14232">
        <property type="entry name" value="GAT_LSB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16980">
        <property type="entry name" value="VHS_Lsb5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044103">
        <property type="entry name" value="GAT_LSB5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045007">
        <property type="entry name" value="LSB5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47789">
        <property type="entry name" value="LAS SEVENTEEN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47789:SF1">
        <property type="entry name" value="LAS SEVENTEEN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VHS" evidence="2">
        <location>
            <begin position="14"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="298" mass="33766" checksum="D692373437D0E183" modified="2017-01-18" version="1" fragment="single">MGVFTDHPHTAITEKINSIIETDLEPDIELPELINTIKLQYSGAIEAARAIRKKLKYGQSKEQFKSLDLLNAMVVNGGRQLEELYNDRKLLERLKLSATDPQINTKLRKKIIILAISWNENFSDKKYYQGIAGLKKQIPTKSYKRKKSTTETGSSSNFMNDEAYNSRSRTNTSQNVDPNVKYKIPKINLQKESPKIKLLIAEASQASINLSNSLKSVNVAKGETSTDNVKATQYFDKSRIIRRKILHYLQLVDSEEFLGSLIHANEELVQALQQYTEYAAKITSHDSDDESLSSYESG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P270</accession>
    <name>A0A1E3P270_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_32875</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038199.1">
        <property type="nucleotide sequence ID" value="XM_019182012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P270"/>
    <dbReference type="STRING" id="683960.A0A1E3P270"/>
    <dbReference type="GeneID" id="30199258"/>
    <dbReference type="OrthoDB" id="2740448at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668:SF18">
        <property type="entry name" value="MALTOSE FERMENTATION REGULATORY PROTEIN MAL13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="10">
        <location>
            <begin position="41"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="606" mass="69020" checksum="8960E93759E4A194" modified="2017-01-18" version="1">MISNNNNNNNNNNNNNNNNNNNNNSSASSGRVSKKRTNTRPCDACAVRRVRCDVATSKSGRCTNCVNHDLECTNIRVRHKSGPKRIQQKTRDNIFSPNLASTNNQNLITCNIPLDKLLPYLQIYQTWYYGLWPVLSVAHLVSKIIDNSVTGQQSSDGKIVLDERNAIPYTLCCAVCATISVQVKFLKSAAGVINVENALSAEEYVQEAKRIRFLFEDRIDPSIDTLLSSFFLHMYYGNVPGGSKRGIVYLREAISMAQMLGLHDPESYQGKSGAEVHRWKKIYYMLLVTERYTCFQEKLPVLLDPTVDMPSLQDEEYPELLLGFTELVDVFAATEKQFFHEINHKLLLSDSDNDKIDADLSMFQDFLQNQSTDMKRQWIGQMQTKLNKKFEQNRISNDSQKLNIVLSKSWFQSLGWLISSENYLINQNESMESCFSAKFPLKIAHDFLTETKDLPVFAFESNGAPGVCNKLLEIADSLYSFSIMSPHQPESTMAFDQLNTIFQMIVKFRSSEVQLPKKLFSKIENLIEAKRITFRQPDPLDLLLDTNEINDEQTQTNESSLNPRNDPIINDDMKNSLSPYTQFTMCYYIPSPNSSNAVQSSQITEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2A0</accession>
    <name>A0A1E3P2A0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidase M3A/M3B catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_84955</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peptidase M3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038587.1">
        <property type="nucleotide sequence ID" value="XM_019185815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2A0"/>
    <dbReference type="STRING" id="683960.A0A1E3P2A0"/>
    <dbReference type="GeneID" id="30203061"/>
    <dbReference type="OrthoDB" id="534666at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06455">
        <property type="entry name" value="M3A_TOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000074">
        <property type="entry name" value="Metalloprotease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.40:FF:000001">
        <property type="entry name" value="Thimet oligopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.40">
        <property type="entry name" value="Endopeptidase. Chain P, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.10">
        <property type="entry name" value="Neurolysin, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024077">
        <property type="entry name" value="Neurolysin/TOP_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024080">
        <property type="entry name" value="Neurolysin/TOP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF84">
        <property type="entry name" value="SACCHAROLYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="10">
        <location>
            <begin position="215"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="660" mass="76355" checksum="6D82F82F55FECF0D" modified="2017-01-18" version="1">MLVPPQAPPSWNHTPEQILKETESYITNGKQLHDKIGAIDQPTIENSFKPFAEFDNENAGISNILTFYQHVSASKELRDASTEAEQKIRDFSIEQGSREDLYKVFNKLFNETKNYDSLTDETKKYIKEINDGFKRNGLALPLEQREKIKEIQKKLSNLSLEFGKNLGEQQEFLLFTKEELDGVPKDVIDQFEKITDDSGEEKLKMTFKYPDIFPVLKYAKNENTRKLAFVGDQNKVPQNEPLLQEAVKLRTELAKTLGYKTFADYILEKKMAKNSETVLSFLDDLQKKLTPLALKEREQLLSLKNKDLKEKGLEQSDQYYVWDHRFYDTLLLEQQYKVDQQKIAEYFPMESTISKMLKIFEKLMNLKFIESIDEKSVWHQDVKQFAVWNTDDGESSPKFVGWLYFDLHPRDGFNKTDDSRQYPVTALVCNFSKPTKDKPSLLKHDEVTTFFHELGHGIHDLVGKTEYAEFHGPSGCSWDFVEAPSQMLEYWTWSKDELRELSSHYETGAKIDDELIDSLIRSKHVNGGLFNLRQLHFGLFDMTLHSINPDTDIAKFWNESREKIALVSSGGVETKGFNSFNHIMGGYQAGYYGYLWSQVFAADIYYTKFKSNPMDTKVGVEYRDIILARGGSKEENDNLKELLGREPNNEAFSKELGLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2C2</accession>
    <name>A0A1E3P2C2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_63548</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038254.1">
        <property type="nucleotide sequence ID" value="XM_019183570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2C2"/>
    <dbReference type="STRING" id="683960.A0A1E3P2C2"/>
    <dbReference type="GeneID" id="30200816"/>
    <dbReference type="OrthoDB" id="3981064at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="303" mass="34897" checksum="2C61AF8F446EA548" modified="2017-01-18" version="1">MARIKHTNRTSKNTNGTVNGNGKKLTKKKVAELPKPVNKAKDEEDIQVVNTVVSCLDIISRQRIEHCVKSARCKHIECFDLKSFCEVNNIINYYEKEHYAFSGKFDVIPAVNHVRSKFTVTDSPRKRQTRFNNATAKEKKYMVTLSQQGKKSPTLFKVVHTKDKSALFPRNLTTKQYQPGWIVRCPLCSIKFCPSELMHDDFMTTVLKYVPKPVRSIEISEDWIVSEVKEKSPFQNQEVVAIDDDDLDNVFVNKNKHSKHKKHKKHRKQVNIGDFDSDDDFDDLMIGELENTGSTMHDPVIID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2F3</accession>
    <name>A0A1E3P2F3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_62255</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. CAR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038876.1">
        <property type="nucleotide sequence ID" value="XM_019183338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2F3"/>
    <dbReference type="STRING" id="683960.A0A1E3P2F3"/>
    <dbReference type="GeneID" id="30200584"/>
    <dbReference type="OrthoDB" id="440553at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF51">
        <property type="entry name" value="QUINIDINE RESISTANCE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="171"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="315"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="408"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="468"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="48"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="510" mass="56197" checksum="47E844DB3EA0B5F1" modified="2017-01-18" version="1">MSNEERLTSGYTSPLVNDSEKKKKEDHNVSTKKLSKHTVFDKSEKYILVLLAASTAIWSSFGSPIYYPALQILENYFDISEELVNVSVVVYMICQGISPTVFAPFADRYGRRPVLIVCLLIFIGSSIGLAVSNSYALILVLRCIQSFGISPAVAIACGITGDWTERKERGWFVGLTSGFTLFGQAFGALIGAALIAAFDWRAIFWFLAIGGCVTLLVMLCCLPETMRSIVGNGSITPKHFIDRAPALLIPYYKRKWDLDDPDYSTLAPQKKTDFLASLKILKKPELAICLLNGAIHYGLWTVQLTSLTNELSKSYGYSVMKIGLCYLPAGVGGLFGSFYSGRILDWVYRREYVKFEDKKKAGLIGEYEKFNIVKSRVLVAVPYTIVTDGFTLMYGWCLAKKVHVSTVLVSEFLISNGCMALVGINTTLLIDLYASNSSAATSCVNLTRCLTGAIFVAALTSMNKSMTIGGTFTLMCALGLISGALLYIPLKFGMKWSAARDAREQRKRND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2G9</accession>
    <name>A0A1E3P2G9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_78191</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038750.1">
        <property type="nucleotide sequence ID" value="XM_019184679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2G9"/>
    <dbReference type="GeneID" id="30201925"/>
    <dbReference type="OrthoDB" id="10599670at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="125"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="242"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="282"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="125"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="363" mass="41815" checksum="3B5B9C17E6BF5E77" modified="2017-01-18" version="1">MFSSISKAWNEVSDESGGGKESSVKFSQSTRDSPRRNGHYIHYDKSKNPLDRIRKTEGKKGSPRILKPSNDNKQNSVWGSALKNLSKVFKKDDGELGSMKEYMNGMIKVPKERKELVRSKRGDTLSIGDLDKDNVNERESRRRRRTDTISSRVESIGNSFNRYKKRRNGDPTEVPKYESYSKFEIDVPKYQYQKETNNGYENYSTAVDTRYHSNETSWKAHTPTDSKPASQGLTFSKITAHDLPSSPIYHNTQSSLSKEHSKKSNLSLENNVDKSYIDSSRIEQLCKKVEQLENTVEGLRDELRQANEKSLQLEKSINSNDHSILKDLDKTDDMPHFKFEDDNNISRPLSPVKVDLDRFKFIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2H1</accession>
    <name>A0A1E3P2H1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Glycosyltransferase family 71 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_24671</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038802.1">
        <property type="nucleotide sequence ID" value="XM_019181046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2H1"/>
    <dbReference type="STRING" id="683960.A0A1E3P2H1"/>
    <dbReference type="GeneID" id="30198292"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF1">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="446" mass="52665" checksum="7C7F69863CD30362" modified="2017-01-18" version="1" fragment="single">FAGPVKENYQQDPIYTKEFLLKFLTLNPKEVQLMKKSHSSMVKSLPKKFPDVLSSNFQPNGYVYVGGSHFNYLVLLSLKSLRASGSVFPVEVLIPKMVEFDVELCNYIFPLYNAKCLVIEDFITKETSKKINKYQMKSIALLINSFKNVMLLDSDNMILKNPDYLFLNEPFKSKGFISWPDLWRRATSPFFYKISGVSINDYKKIRNSYWHILKKQFHDIKPDWLYSYHDFEGTIQETSSESGQLLINRETHAKTIFLSTYYNFYGPDHYYPLLTQNSHGEGDKETYISAAHALGQTYYQVEEFARDFHKNHHRVKFGRQRDIIGIGQYDPMIDYNRSTLMTLEESKRNDVDQFKYSSGHSIWEYKIWNQSELMFLHCNWPKLYTWDLINSKGIRSFKNAKGKRQRLYDGLINELGGKRDFELEIVGYMKWYFCEHKDLQIRGVFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2I1</accession>
    <name>A0A1E3P2I1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Karyogamy protein KAR4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_15577</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MT-A70-like family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038667.1">
        <property type="nucleotide sequence ID" value="XM_019180650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2I1"/>
    <dbReference type="STRING" id="683960.A0A1E3P2I1"/>
    <dbReference type="GeneID" id="30197896"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000742">
        <property type="term" value="P:karyogamy involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045123">
        <property type="entry name" value="METTL14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13107">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13107:SF0">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51592">
        <property type="entry name" value="SAM_MTA70L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="34393" checksum="451B891E0A42D831" modified="2017-01-18" version="1" fragment="single">PQKKFKDNDYSNNYIHTGELPSKFVRNVKNPVEGYPKLQRLFQLKERQTQAYACTPFGSRVDIDNMVPTLNKWIHKDKLVFDVIMIGSLTENQFIYPLLTQLPLEKLCSKPGFLFIWASAQKINELTRLLNSDGWAKKFRRSEELVFVPVNKDSPYYPNRDGIEDDSLFEKMQWHCWMCITGTVRRSTDGHLIHCNVDTDLNIENEEDNNKNLVVPNHIYKVAENFSSANRRLHIIPSRSGYNNPVKPRRGWVIMSPDIIIDNFEPLRYKNEITEIGTNVPQDNEIENLRPKSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P308</accession>
    <name>A0A1E3P308_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_32889</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038474.1">
        <property type="nucleotide sequence ID" value="XM_019182014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P308"/>
    <dbReference type="STRING" id="683960.A0A1E3P308"/>
    <dbReference type="GeneID" id="30199260"/>
    <dbReference type="OrthoDB" id="4036490at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012470">
        <property type="entry name" value="Pup1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07954">
        <property type="entry name" value="DUF1689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="274" mass="31526" checksum="E53A049E22DB493E" modified="2017-01-18" version="1">MDLAVKFYEADQQLDRNDRELLGKLYTKVTYSTLVFGWGAFFAVASIPFYRQKLKTGSRKGTNVGRAFLLGFAGMIIGAPIASKYSYNWQVDSLQDTNSKCYQVAKILKPSEAVKWMMYYRLTADHPEHIMKDPRSLEAAKQRDRKLMQNRDPMGLYSGPRFDMQQGKKNKQDQEEAVKEEGEIEGTSNISSWDKIRLQNGMKESGTSGGGSAWNKVRQQQSNTTTQEKPVNPFTTNVYKPEQTKSFDEQENEFEKLLENERHIGEEEEKSNKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3E0</accession>
    <name>A0A1E3P3E0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">GPI ethanolamine phosphate transferase 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_31074</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the GPI second mannose.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the PIGG/PIGN/PIGO family. PIGG subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039208.1">
        <property type="nucleotide sequence ID" value="XM_019181794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3E0"/>
    <dbReference type="STRING" id="683960.A0A1E3P3E0"/>
    <dbReference type="GeneID" id="30199040"/>
    <dbReference type="OrthoDB" id="272139at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051267">
        <property type="term" value="F:CP2 mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16024">
        <property type="entry name" value="GPI_EPT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000045">
        <property type="entry name" value="GPI ethanolamine phosphate transferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037674">
        <property type="entry name" value="PIG-G_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039527">
        <property type="entry name" value="PIGG/GPI7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045687">
        <property type="entry name" value="PIGG/GPI7_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23072:SF0">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23072">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19316">
        <property type="entry name" value="PIGO_PIGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 12" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="436"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="487"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="570"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="610"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="650"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="689"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="736"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="771"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="GPI ethanolamine phosphate transferase 2 C-terminal" evidence="13">
        <location>
            <begin position="376"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF19316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ60001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="796" mass="90743" checksum="40331001CD2186DF" modified="2017-01-18" version="1">MRWISVLTLTVLQIAGLLIFCLGFFPSKVVLKGFGQFDNGQYDPQFNKIVVMVVDALRSDFIFSEDSHMYFVQQLLQQGSAIGFTAYSNPPTVTLPRLKGISTGSTPNFLDAILNVVEEDSSSTLANQDSWVTQLKKAGKRIHMYGDDTWIKLFPSVFDKFEGTSSFFVSDFTEVDNNVTRHLDSELKEQNWDCLILHYLGLDHIGHKGGPKSIFMPPKQQEMDLIVKRIYKSLQEDTLLVLLGDHGMNDLGNHGGSSAGETSAGLVFISDKLKKLKTNSNVPPPNRDDFTFLQRVQQIDLVPTISGLLHLPIPRNNLGIMIRDFLPLWNKEQQFNLMKENLKNFERLSENPLIAQDIDSAFTALQEAQSDLTRSATNYDTQKIFIGLGIVILTSLLILINFWNTVGLKVVVFYTLNIVYGATMFGSSLVEEEYQVWWWVLTFILTVLCFNKNFNFFALIFLLRIIRGWNNSGQKFVGDTILNFLQENYIINWILVFFTISIYGFNLNYGGISYYHPVISFVISFLLSITTFTFKLLFSLANGEKIPKELVQLAAYSIDKLGASNLQESLVDIARLFFVTVLFVFGWRFFLKFKNHDYWYFTDLHNLITFVLIFQSSLQNIPLFLVFLGVKHQIGVVTGGLTKTNKLFKVGLVSLSILVLQQFSFFSTGQTNSLATVDLSNAYNGITGYNIFAVGLLTFISNFAGPIYWSFASLPILFENKENLKIVKYEIIKMRWLVNASFYSISGVFTLLACYMLRYHLFIWTVFSPKLLYTIVWNIFMNVCIELVCLVFTVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3N1</accession>
    <name>A0A1E3P3N1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_78576</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019184754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3N1"/>
    <dbReference type="STRING" id="683960.A0A1E3P3N1"/>
    <dbReference type="GeneID" id="30202000"/>
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    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019436">
        <property type="entry name" value="Say1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF31">
        <property type="entry name" value="STERYL ACETYL HYDROLASE MUG81-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10340">
        <property type="entry name" value="Say1_Mug180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133650" description="Alpha/beta hydrolase fold-3 domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="347" mass="38706" checksum="0BAE68709C3C07E8" modified="2017-01-18" version="1">MKITLIPIFLSIIHVTLSAPALTFPTPKIGEDIVKYTEAFLSIFITLLDNNFPVVQIGNLVPDLSLDFNMKVVEPFAKDLPNYNTEFPVEGLDQGKVKWLVEAENRTKDDPVIIYSHGGGYVFGVFPFFPFLFTRIYQETGNNRLSILMLDYKLSLEEKYPYQLGELALVYNKIAESSNNIILAGDSAGVHLSLNLLRHSKFPVSGVNQVSAGNITGGLFFSPWVNMYVDDTTGSHEYNMGKDILTPKIIREGGKQFVPSSNLLTSNVVNFYNDTEANWKELVPKNVLATYGGHEIMETDDKSWFELAGLSGDQIFEDKGAIHDAVIFTPDLSAVVPKAVEFLKTNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3P0</accession>
    <name>A0A1E3P3P0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_31492</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039313.1">
        <property type="nucleotide sequence ID" value="XM_019181839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3P0"/>
    <dbReference type="STRING" id="683960.A0A1E3P3P0"/>
    <dbReference type="GeneID" id="30199085"/>
    <dbReference type="OrthoDB" id="2159336at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110085">
        <property type="term" value="C:mitotic actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990528">
        <property type="term" value="C:Rvs161p-Rvs167p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060988">
        <property type="term" value="P:lipid tube assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072741">
        <property type="term" value="P:protein localization to cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030100">
        <property type="term" value="P:regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07599">
        <property type="entry name" value="BAR_Rvs167p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000048">
        <property type="entry name" value="BAR adaptor protein RVS167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000189">
        <property type="entry name" value="BAR adaptor protein RVS167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046982">
        <property type="entry name" value="BIN3/RVS161-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174:SF1">
        <property type="entry name" value="REDUCED VIABILITY UPON STARVATION PROTEIN 167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="7">
        <location>
            <begin position="17"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="404"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="289"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="463" mass="51615" checksum="58189513936903C7" modified="2017-01-18" version="1">MSFKGFTKAINRAPQNFRQKFHMGTSTSDPVYEDAERRFKEIEVETQKLSDESKKYFTAVNGMLNHQIGFSKAMEEIFKPISGKLSDPNSTIPEDNPEGIEASTQYREIVADLQTTLKPDLELIDTKIVKPAQELLKVIQSIRKMSVKRAHKQLDLDRHKNTYMKYENKKERTPKDEEKLYKAEADVQIAQEEYDYYNNMLKEELPILFQMEAQFVQPLFVSFYFMQLNIFYTLYNKFEDMKIPYFDLNTDIEEAFAAKRGDIEERVAEIGITHFKVGHARAKLEMTKRRYGKGGEESPGGVVSPTSTGSNLPPYTPPPTGAAGGYGLTPQGYPDEKKDPYGQQPAYGQQPAYGQQPAYGQQATPQSYGAAPAYATPPTQYSSPAPAAAVPQYGSPAPAPVPAPAAETCTALYDYSAQAEGDLSFAAGTVIEIVQRTADANGWWTGRANGVTGVFPGNYVQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P439</accession>
    <name>A0A1E3P439_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Autophagy-related protein 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_62248</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Autophagy-specific protein that functions in response to autophagy-inducing signals as a scaffold to recruit other ATG proteins to organize preautophagosomal structure (PAS) formation. Modulates the timing and magnitude of the autophagy response, such as the size of the sequestering vesicles. Plays particularly a role in pexophagy and nucleophagy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Preautophagosomal structure membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ATG17 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038869.1">
        <property type="nucleotide sequence ID" value="XM_019183337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P439"/>
    <dbReference type="STRING" id="683960.A0A1E3P439"/>
    <dbReference type="GeneID" id="30200583"/>
    <dbReference type="OrthoDB" id="1937984at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007240">
        <property type="entry name" value="Atg17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045326">
        <property type="entry name" value="ATG17-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28005">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28005:SF1">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04108">
        <property type="entry name" value="ATG17_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Autophagy protein ATG17-like" evidence="8">
        <location>
            <begin position="16"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="151"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="424" mass="48995" checksum="5435248EB5A2C574" modified="2017-01-18" version="1">MEQEVQKWLEDATKKLSAAETQGAKANTLLTTTKSTISSNNGKLIKSNFLLDSINQLVQILETIHDSVELRLNDRRQSVKALKNYLELSLTELRKEFDKLKQIKIDANLLSGDHRTLYDFVSNEVLETLLSKQDELEKSRESYVVVIEQQQEALKTKLNGFNKQFNQLEKDVTEVNDAGWISDLIDENNKLEEEVAGLLESLTNHYDQCSRGARIFDGEEPEISEVEKTELFEVLAKDFREVPDVLNDLEECIEDIEQRCKKIDSYLSTKFYDIKLKHLVQDLTSFGENELMQVMSTLDKQTIDFQAVSTDINEKCDEAHQLINHYERFVESYYRLILELDRRQGVQLSMEKVFNEAKTKLQALQEDDYKKRDTFFQTNGSYLPNDLWEGLNDDSPLCNLKFEIHGLPEINPSTLATARKNIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4C3</accession>
    <name>A0A1E3P4C3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial presequence protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_62911</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039556.1">
        <property type="nucleotide sequence ID" value="XM_019183459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4C3"/>
    <dbReference type="STRING" id="683960.A0A1E3P4C3"/>
    <dbReference type="GeneID" id="30200705"/>
    <dbReference type="OrthoDB" id="4953at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000015">
        <property type="entry name" value="Putative zinc metalloprotease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000031">
        <property type="entry name" value="Putative zinc metalloprotease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016:SF16">
        <property type="entry name" value="METALLOPROTEASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G07610)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016">
        <property type="entry name" value="PRESEQUENCE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="2">
        <location>
            <begin position="52"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="3">
        <location>
            <begin position="194"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1002"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1010"/>
            <end position="1042"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1042" mass="117884" checksum="CE1E0BA9517EF4E2" modified="2017-01-18" version="1">MTGSNFELLTSFDVEYAPSTIKKWRSKRTGLQAVLIDKESPIVNGFFAVATEVEDDSGTPHTLEHLVFMGSKKYPYKGLLDTLGNIAFSSTNAWTATDQTVYTLTTAGWECFKLLLPIYLDHILNPTLTDEACYTEVYHVDGEGKEKGVVFSEMQGIQNQPWFLSALNSSRALYHEKSGYSSETGGLMENLRKLSNDTIRKFHQSSYRPNNLCIILSGTVEEDEFLKTLDEFDSELPELGNDNYRPFVDSKYPIVPLDKEVVKNIEFPDKDESHGELSISWVGPKNEEYEQDLAITLLLEYLSESPVALLTKNLVEIDDPFASEIEYNTDDYTRIIPYIKLNDVPTEKLEQAKDKVFQLLKEHATIENFDLPRIKEIVENSKHKLIFATEKSEDILIDYTVTNFLYSDVESLTKNLKTMHDYETISKWTIEQWVELFQKYLINNHAAVVIAKPSKQLYKQVKDEGKKMIEDRLADLGDEGLRKLKGKLDEAQAKNDAPIPENILNSFPKPDPSKIKFIKTTSAAIGLNKDYENDLSNETVSRLVEDTPKDFPLYINVEQFKSQFISIDILMSSFEIPVELLPYFDLFSELFSLPLEIGGERIPYEQVIKDLQNDTVEHSFYTAYNNQFTEFVNAKIQVKKANYTKAIDWFKKIFFNTKFDENRIKVTVDKILNGLPSTKRSGLSMLSSITSRNCFTERSIKHSKDSLYTENFFRNLSKKLEDGGYKEIEQDLETIRSKLFKTNNFRIVISGDLSDIEHPIKAWEHFIEGQDLANDSLISVPRSFDVRTELGESLSKKVKIITAPASESTFLLASTKAPTDYHNEDAAAISLAASYLQAVEGPFWRGIRGTGLAYGANVSRSVETGQLSFDIYRGADAIGAFNVASKIVNDLASGATKLDKTLVEGAVSSLVNSIASSESNYYNAAASKYLDNVLKKRGPDFNTKFIRKLSQITEDDLIRVLNKYYVPLFDSKTSVIFVCAHPSKSEELNKFFSGLGYEVSVEEVPSSNSEDGDEEGSEFDSEEYTEESGSDSEEEETDSDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4E2</accession>
    <name>A0A1E3P4E2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Survival protein SurE-like phosphatase/nucleotidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_78747</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SurE nucleotidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039337.1">
        <property type="nucleotide sequence ID" value="XM_019184781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4E2"/>
    <dbReference type="STRING" id="683960.A0A1E3P4E2"/>
    <dbReference type="GeneID" id="30202027"/>
    <dbReference type="OrthoDB" id="4018688at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008252">
        <property type="term" value="F:nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="entry name" value="Survival protein SurE-like phosphatase/nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030048">
        <property type="entry name" value="SurE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00087">
        <property type="entry name" value="surE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457">
        <property type="entry name" value="5'-NUCLEOTIDASE SURE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457:SF0">
        <property type="entry name" value="PHOSPHATASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G01070)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SurE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133653" description="Survival protein SurE-like phosphatase/nucleotidase domain-containing protein" evidence="4">
        <location>
            <begin position="20"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Survival protein SurE-like phosphatase/nucleotidase" evidence="5">
        <location>
            <begin position="22"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="329" mass="35738" checksum="E8DE278C733C3C78" modified="2017-01-18" version="1">MKLSSILTALTTVVSTALAKNIVLTNDDGWASTEIRATYRSLTQAGHNVYLVAPLEQRSGFGGQFVFSFTNTLLHDDQFHFKKAGDPAWGHEPNDDHIWYFNATPAACVGFAFDYLLPTYFSNVSIDLIVSGVNQGLNLDDSMFTISGTIGATYNAIYRGHSAIAFSGSTSNNSFYKDSLNEDPNDPANIYASKVVELTSKVFESQGENERALPLGVGLNVNFPKVTTLTKDNSCSNPPFVFSRLTGKDVAISALKFNETTGLFEYSSIKHGTDATATRYNGILSLPNENSVINTGCYTPVSAFSIDYTAPIEQSNEVHGLISDLLVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4N1</accession>
    <name>A0A1E3P4N1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="7">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_30082</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="7">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="7"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039648.1">
        <property type="nucleotide sequence ID" value="XM_019181686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4N1"/>
    <dbReference type="STRING" id="683960.A0A1E3P4N1"/>
    <dbReference type="GeneID" id="30198932"/>
    <dbReference type="OrthoDB" id="3370at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17956">
        <property type="entry name" value="DEADc_DDX51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="166"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="391"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="36"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="565" mass="63823" checksum="7AA067C3794D9606" modified="2017-01-18" version="1">MDSGSDESESSDSESESEDSSDESNSEDETKDEVEVEKINDEPEIQQDHDTKFSSIISRFNKSIAKPKQDEEESEESDDQTEKHELIPLPQPELPRDQRLSRALTNNSLNWLANPQYIKTDSTKPFNEFDISPKILKNLQDLGFDNAFATQIKTLDLLLPEINNKLNPNSIKGDLLVNASTGSGKTLAYTIPIIQSLQNRIVPKLRCIILVPTKPLINQVYKTLISISKGIDLNIVTLGKTDLNLQDEHLKLKTNVPDIIVSTPGRLVDHLNLQSIELKNLQWCIIDEADRLLNQSFQDWSNVLIKNLNSINEGNNLSKIYKPNLIKMIFSATLTTDSGKLSNLNFHNPRLIIVNNEESILQNDKIFTLPSQLSEHVVKLSSSSSSHKPLYLLKLLESLQFPHNVLIFTKSNESTLRLSRLLSLISNKLQIPLIISNINSSQSRSEKSKLLTQFAKGEIHILISTDLISRGIDILTIQHVVNYDLPNSSREYVHRVGRTARANNKGDAYNFLIGKGEQNFWNKFNLDINRGDNEISEIELQDIDSMKDAYVESLGELEKEVFATN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4W8</accession>
    <name>A0A1E3P4W8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Centrosomin N-terminal motif 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_79059</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039672.1">
        <property type="nucleotide sequence ID" value="XM_019184835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4W8"/>
    <dbReference type="STRING" id="683960.A0A1E3P4W8"/>
    <dbReference type="GeneID" id="30202081"/>
    <dbReference type="OrthoDB" id="10255522at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012943">
        <property type="entry name" value="Cnn_1N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19327">
        <property type="entry name" value="GOLGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19327:SF0">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07989">
        <property type="entry name" value="Cnn_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centrosomin N-terminal motif 1" evidence="5">
        <location>
            <begin position="121"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="91"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="121"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="91"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="966" mass="112926" checksum="4D44108141B1A5F2" modified="2017-01-18" version="1">MFKTKPLIGTPNDSRVGNKFEFTPIGYKVNDENRDPRTNTIKRTSKLNPMSTIKRNRDPFNGSPLANSVRGGSSFIEPESFDTIHYSDNGRQQISKQHKTSSPVPNGFDNHDLLNNGSNPVRQQQENVSKLQDENYNLKVKIASLTRFLNSITNNEQQEIYQQNSELQEKLIEMRGQISLLNQELSDSKKSTNSAANDVEVEKFKDEIRSLNKIIQELREATENQHELEREVNKLHQVIEELETENIKEKQSSISPAYVDKLEEQLEEVKERLHDVEFELNRKNEELDGKDDKLEEMEEILRKHKSLSDKQQDQIEDELEDMKDAIRAKDKEIDSLMDKLDAKDSLLAQLKNDYDSLKEKLQNDYAKLKDGQVESNKFQQKLKELEIEKNQKNDQLKQSQSKYSETLRELSDLRHDLASANDMMRKQENVIDELRSTKTSNEKIYKFADDLKVKVKSLEKELHSLSLERDVLQKQLAKSDQEIIGLKNNNERTYKAYNELKKKQQQNQSTQATTSNNEQFWKSEIDQLSNQINDLNVENKKLSRELQNEKVLKKLDVDSYEKHEVKNLTARCNDLQLELSEKENLFNHTVNKYKNEIENYKSMIREKEDELRKVSSELRAIKYSTTQQLDEEKLEALKVKSSKEYQIKAMKIELDNLKEEHDAEVKSYKKLIERLKNASSGKDVDDVNSRLVSNTANETDQLLQKINDKNNKIRILNNKLTEAVSNVKVLEKEVSRLEGVKLSLLHDNKKLDAKIDLLSDELVSQRKETERLLSQATDPSTIYELKRELKLRENQLEASNKEFNEMKHDLINKYREIHDEKSSLERRVEKIIKNYRQLQNNLNGKVFDNKSPSLSSKELIMTKDQADFYRMKYNKSTYVINDLKFMNSFMLKSIQATNSHIKKDVKKLQTAGIYPDYELICNKKPSLSVLFKFVVAAVRIKRKTEHSGIRNKKLENLKFKLELQDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4W9</accession>
    <name>A0A1E3P4W9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_29186</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039728.1">
        <property type="nucleotide sequence ID" value="XM_019181585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4W9"/>
    <dbReference type="STRING" id="683960.A0A1E3P4W9"/>
    <dbReference type="GeneID" id="30198831"/>
    <dbReference type="OrthoDB" id="206700at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045913">
        <property type="entry name" value="TBC20/Gyp8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913:SF7">
        <property type="entry name" value="RE60063P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 20/GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="56"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="266" mass="31059" checksum="B38B921EBA0E2F29" modified="2017-01-18" version="1" fragment="single">MRVETLDWDTISLKLENSINDPKINQRNLKESSLSTAVEMQDYKILQYLTKTKGGLITSEFRKVIWPVLLGVEHLEDEVEPDWRLLPQHKDEDQVGLDVERSFVYYPQNIPSTDKRKLKDDLRSIITRVLRTYPELSYYQGYHDICTVFLLVFEDDLELCFKVLSIFTLVHLRDFMMPNIDVTLKMLKLIPELTSKIDEDLYNDLIMGNLEPFYGLSPIITLFSHDITEFSTICQIFDLIIANGSIGVTIYIYVCLLSNKKDKIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4X4</accession>
    <name>A0A1E3P4X4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flo11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_84247</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039575.1">
        <property type="nucleotide sequence ID" value="XM_019185675.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P4X4"/>
    <dbReference type="GeneID" id="30202921"/>
    <dbReference type="OrthoDB" id="3982858at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133667" description="Flo11 domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="731"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="817"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="885"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="949"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1079"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1206"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1310"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1079"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1101"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1121"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1141"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1206"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1230"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1260"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1310"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1320"/>
            <end position="1330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1392" mass="145274" checksum="BF099992FF192C89" modified="2017-01-18" version="1">MKLLNLLYATFIWSFAYCHYIQRESTPEVVSGITTVTTGLGLSTTLTSECAFATHSGSINGKRFKAAYEISGETTTSVVAAIPFRNRELTVTWLFTFDEPLDANDFKVRQLGVDISSIDGYVNLEEGSFLVTYQVTLPFITYLTSAIDYCLFEYVAFDFHKVDSAGNDFFYLLYTDNLGSALVDGLRGAVCDIFTATNCPIYQAQLPFDCEAFKKLTRCDGHTTRTIQPSGVSSTKKLPTASVTSHTTTISSRSVAKDVTTALPAKPLATTPSVVSGITTVTTSLFAEETYTSQCKYSAQTGSINGKHYKAAYEISGKTETEIVAGLPFHNRILTVTWFFEFDKSIDAKSFRVRQLGFDISAISGTVNLEQGAFVVSYEVKIPLITYLATAINYCLFEYVAFDFHKVDSAGNDFFYLLYTDNLGSALVDGLRETICDVFTETYCPLYQSQLAFDCDAFKRFDKCDAVTTITLGSPASSATSQIDQVTSSNTLAISSGSPVAHGDSTSEASSILVTSGSTTATIASTTPDVVSGITTVTTSVGISGTFTSECDFETETGSINGKKYKAAYAISGETTTSTVAGIHFHNRVLTVIWFFQFDKPLDANDFKVRQLGIDISSIDGEVNLEQGAFVVSYQVTLPFITYLTSALNYCLLEYVAFDFHKVDSSGNDFYYLLYTDNLGSALVDGLRETVCDLVTLTNCPLYQAQLAFDCDAFKDFQSCHGATTFPGLPTSSSSTVRITSSSSSTKSSSSFVASSSSAKTSVSISIHSSDFVRTNSLSSAASSSFVSSPASMSSGQSSSLGLSTGSTSIKASTLSSSSLSSTMPNSSSSLSSSSSPATSRLVSSSSSLTRSASAVSSMSSSTTFSSVSLISTSSRILSIGSLSSHLNHDSSTVSSSTYRESASQSSSSSLPSTSNSMVTSSCLVSFSRELASSSSSSSKLSVGLASDTSASTSSGSTSEGASTTSSAFSSNTDSYQYLTSSTSYVSYIASVSSSSTLSAPFPFTNSSSTFNTHVQGSSSATSISTTLSGDVHLSSAASLTSSTPGTSSTASSLTFGAHSSKNTNVISSSSTAVYESVSTNLPSSSSSSSNALETSALNSPGSTSTDILPSSSIPDEFPLTGTNAHGSIATSTDKSRDTADSTMKSSSGTNFAISSPLSASSGILSYITSTDSAGNIETGAVKDVTKTGSAGETYHASTTVFSSSKAYSTSTGSSGSDVSNSSNKDDGKANNGNSNSSSQDGISSPNSSSASGYHDHKSSSSGSNTNTAGGSIAGSQNDHHGVSNSGSGSSNIAHTLSSIPLSQVAVSFSSSGSESSNSNDKYGSSNASIDRETLTSTTVQVTKFQIASDTSPSKLQDSSTTPLVSEFEAKAVSNAGNIFLGLISFVLILFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5C5</accession>
    <name>A0A1E3P5C5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_63034</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039687.1">
        <property type="nucleotide sequence ID" value="XM_019183480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5C5"/>
    <dbReference type="GeneID" id="30200726"/>
    <dbReference type="OrthoDB" id="5361617at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="2">
        <location>
            <begin position="9"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="296" mass="33870" checksum="6D3A7933BBEE0535" modified="2017-01-18" version="1">MTEEIQGYFIIDASALTKGLGNIKRWIDEQNYNVKLFIPSYTIHELDYLKKGMSMLATNARESIRFIDRVVSSEDHHIGSKLILETPDQVGPNWRKCMTFKVRSPKTHEFPNNKNVTSKPSVIKYENEEDDDEADERGNQSNEKNLKKERAELPARYKYLLRPCIKKTHVDPEDWKLITEDLTTKIWCESFGIKCINVSEAESLLFQLKNKTQIGNDTNYTFSKLSIEDEPRPLSSSSSSSNGGKRKRSRARKSNNNNANIINDINNTGGDSQNTHRESYDSISFAPRGSGELWVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5Q9</accession>
    <name>A0A1E3P5Q9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Homologous-pairing protein 2 winged helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_67454</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HOP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039988.1">
        <property type="nucleotide sequence ID" value="XM_019184084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5Q9"/>
    <dbReference type="STRING" id="683960.A0A1E3P5Q9"/>
    <dbReference type="GeneID" id="30201330"/>
    <dbReference type="OrthoDB" id="272266at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120231">
        <property type="term" value="C:DNA recombinase auxiliary factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120230">
        <property type="term" value="F:recombinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007129">
        <property type="term" value="P:homologous chromosome pairing at meiosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000709">
        <property type="term" value="P:meiotic joint molecule formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010774">
        <property type="term" value="P:meiotic strand invasion involved in reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010776">
        <property type="entry name" value="Hop2_WH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15938:SF0">
        <property type="entry name" value="HOMOLOGOUS-PAIRING PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15938">
        <property type="entry name" value="TBP-1 INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07106">
        <property type="entry name" value="WHD_TBPIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5" id="KW-0469">Meiosis</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homologous-pairing protein 2 winged helix" evidence="7">
        <location>
            <begin position="15"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="86"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="211" mass="23823" checksum="CE59526F9B7FE563" modified="2017-01-18" version="1">MPPKKEKKVQVKGDEAEKTILEYLEQQYRPYSVNDIIQNLHGNISKAAAVKALENLTAENKIVTKVLGKMSISVANKVEVDQSEESINYDEELEVMKQNIKEASQNLSALQTELSETTKYPSDEELDAELKSLQKEVEDLSHKIEAAKSKPSISTESVNKIKSLSASITSESQKRQKLYKNFLKYIAEFIDPKKLIFDLGLEEVTMVQNKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5Y2</accession>
    <name>A0A1E3P5Y2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_83173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040063.1">
        <property type="nucleotide sequence ID" value="XM_019185501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5Y2"/>
    <dbReference type="STRING" id="683960.A0A1E3P5Y2"/>
    <dbReference type="GeneID" id="30202747"/>
    <dbReference type="OrthoDB" id="10263222at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090730">
        <property type="term" value="C:Las1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007174">
        <property type="entry name" value="Las1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15002">
        <property type="entry name" value="RIBOSOMAL BIOGENESIS PROTEIN LAS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15002:SF0">
        <property type="entry name" value="RIBOSOMAL BIOGENESIS PROTEIN LAS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04031">
        <property type="entry name" value="Las1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="412" mass="48544" checksum="F970CD982B5D8B5A" modified="2017-01-18" version="1">MNKHPRQVPFRSQKDIHFLKTQLFAPESTLSTQTRAIQRILTLETKTKLPHTLLSTAYLFQIHHEFQSNPTASTVLQLSATMTLIRFVNGLLDPSQQSQFAIPLNLLAKKIGLPSWFVELRHAGTHEGLPSLEMLEIGVLGALDWLKVNYWEKIKSEEEEEEEEEDVDDEDWKDVLRKYRRIRRVDINKFIKFGDSSEEGKEYWKVVKVLESGVLKNEFYKVLMFKKCIINEKYKWEQVRLLYEPLFLYLIKSQESFVLKILDKLMFKVYEDENKLVSVDEEEVYNGKEIQQVKSWCLWILEKINNNEDNIIGKMVEVLSKTNQSFSIEMLERLKLKNVEVDKINLKINTIQSNLKEKNNITPSKRKEDDDLDIFQDLENLKKRVKLNKSTNLPIKIFEPHSNWIPKPFGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P608</accession>
    <name>A0A1E3P608_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">fructose-2,6-bisphosphate 2-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_28583</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the phosphoglycerate mutase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.46" evidence="2"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040068.1">
        <property type="nucleotide sequence ID" value="XM_019181510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P608"/>
    <dbReference type="STRING" id="683960.A0A1E3P608"/>
    <dbReference type="GeneID" id="30198756"/>
    <dbReference type="OrthoDB" id="267323at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000005">
        <property type="entry name" value="GpmB, Fructose-2,6-bisphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000644">
        <property type="entry name" value="GpmB, Fructose-2,6-bisphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF44">
        <property type="entry name" value="6-PHOSPHOFRUCTO 2-KINASE_FRUCTOSE 2,6-BISPHOSPHATASE LONG FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="7">
        <location>
            <begin position="5"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="229"/>
            <end position="236"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="462" mass="53475" checksum="390E953E5355EE22" modified="2017-01-18" version="1">MPVYTVSHVQNIRICVVMVGLPARGKSFISQKIVRYSSWLSIKAKSFNVGSYRRQTEEHPDAEFFDSHNEAGMKIRQEAALHAVKDMMTWFNEEQGVVAILDATNSTKERRDWILRLCRENSIEPMFLESWCNDEKIIKKNVLDVKTTSPDYAGQDPDVAAKDFMKRISKYEDVYEPLDEVVSKDLTFVKLINVSQQVIVNKIETYLESRIVYYVMNLHIKPRCIYLSRHGESMYNLEGKIGGDADLSERGFKYAQKLPELVKQLAGEDHKKLTVWTSTLKRTQQTAQHLPYNQLQWKALDELDAGACDGLTYEEIERQFPQDFKERDENKYEYRYRGGESYRDVVIRLEPIIMELERQENILIITHQAVLRCIYAYFMNVPQEESPWMSIPLHTLIKLEPRAYTTLVERLKADIPAVSTYKERGTSQLGEREDAKNKSKHLITGEPVKVKTQVAAANASVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P653</accession>
    <name>A0A1E3P653_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_20299</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039967.1">
        <property type="nucleotide sequence ID" value="XM_019180863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P653"/>
    <dbReference type="STRING" id="683960.A0A1E3P653"/>
    <dbReference type="GeneID" id="30198109"/>
    <dbReference type="OrthoDB" id="427030at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF11">
        <property type="entry name" value="ZINC FINGER PROTEIN YPR022C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="7">
        <location>
            <begin position="51"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="583" mass="67195" checksum="CA351D5A62B2FE09" modified="2017-01-18" version="1" fragment="single">LELLESDLGNIPSFQGNSTLITSELMRRMLFLIPTLTSHEDFDATALELCLNIYWTTFHIQYPIIHKPSFNTLTAPPILILAMVLMGAALSNSIGNPFKDPLGMAMEIGIPLRWLIFKHRSPGASEPWEIQSLLILEVFEKHYSSRDLHERSSVHYAAKIEMMKRSTVLGGDPYATESKSHTELNETDQYVLWNKWIHAESMKRCALMAFCFDLNSLITSGHQASLYVNKLRLGLPCDDCLWEADIDTLKNLELPKNPEMVLTALKKLLKGEKVQTNSFGKKVMLFAVVSLMVQFELRDETMAMITDESTSDILKDVWRDKLSYALDSWKYNVNEGSCCDVSTLLVDLRVRKADSGTVYFDLNDTKCKYPSYHMAQIRLRIVNHDMLILAGLPLRMSVKVDDKDYKNVEMRLRKWAKSLDGRVSVVHAYLCVIECLLNFQDGVIDYRPDKDPVHERAHIINASVMIIWCYNFLERGPESDKFNEGLEEYNEDAFDYLVRIKEELDRNLVLDRGAFEYHMSIRKCAANLSSIDNLNNLASFFNIVSSLYTQCFWELGVEYSRLHHHCKERFMGKQDPFCKDMYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P697</accession>
    <name>A0A1E3P697_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_83112</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039991.1">
        <property type="nucleotide sequence ID" value="XM_019185492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P697"/>
    <dbReference type="STRING" id="683960.A0A1E3P697"/>
    <dbReference type="GeneID" id="30202738"/>
    <dbReference type="OrthoDB" id="415023at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22762">
        <property type="entry name" value="OTU_fungi_OTU2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049771">
        <property type="entry name" value="OTU2-like_OTU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF10">
        <property type="entry name" value="DEUBIQUITINASE OTUD6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="2">
        <location>
            <begin position="169"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="304" mass="35349" checksum="1415868C32FDD92F" modified="2017-01-18" version="1">MSEFETEDELLARHRKENKELIANITGLKKQATKSKKKEVQRKCADMELKLKEKHEQELKELRSRGGDGNLEESKQVEQDEDEDDDELTPEKLLAQLELEKKEEEPKQQQQKQQQQKGPKRNRQKERLAKRQAALDEQRKQAEEEASLQPDFKKIEDENIKSLCSIAKLKEFDIKPDGNCLFASIADQLKIRQGVEVDIKTLRTQAANHIRKDPDTFIPYLFDEETMSLKNIDEYTEKIEKTAAWGSDLEILALSKEYDSPISVMMTGRSTLKINEDGNNEELKLVYYKHSYALGEHYNSLHDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6J1</accession>
    <name>A0A1E3P6J1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-dependent oxidoreductase 2 FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_61438</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040081.1">
        <property type="nucleotide sequence ID" value="XM_019183174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6J1"/>
    <dbReference type="STRING" id="683960.A0A1E3P6J1"/>
    <dbReference type="GeneID" id="30200420"/>
    <dbReference type="OrthoDB" id="10254877at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050315">
        <property type="entry name" value="FAD-oxidoreductase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43400:SF7">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE 2 FAD BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43400">
        <property type="entry name" value="FUMARATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="5">
        <location>
            <begin position="8"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="233" mass="25944" checksum="E5D179C551F97219" modified="2017-01-18" version="1">MSQPTLPVVVVGSGLAGLTSVYKLLERKIPVILIDKMDKMGGNSIKASSGINGVLTSTQKQFGIEDSVQGFFEDTKKSGGKIGDLNLQQKLVKESTEAIRWLQQDIGVPLDLVNRLGGHSQRRTHRSSNIPPGFEIISKLKNKIVEHEENLKLLLNSQIVDIDISNGKIIGLKVLDLINKHEDYIKTNQIVMATGGFGFSEELIKKYRPDQINNFRIHQKNKTIKQFTQNKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6K7</accession>
    <name>A0A1E3P6K7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">General transcription and DNA repair factor IIH</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_27932</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the GTF2H2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040101.1">
        <property type="nucleotide sequence ID" value="XM_019181425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6K7"/>
    <dbReference type="STRING" id="683960.A0A1E3P6K7"/>
    <dbReference type="GeneID" id="30198671"/>
    <dbReference type="OrthoDB" id="284275at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01453">
        <property type="entry name" value="vWA_transcription_factor_IIH_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000477">
        <property type="entry name" value="General transcription and DNA repair factor IIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000015">
        <property type="entry name" value="General transcription factor IIH subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007198">
        <property type="entry name" value="Ssl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004595">
        <property type="entry name" value="TFIIH_C1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012170">
        <property type="entry name" value="TFIIH_SSL1/p44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
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        <property type="entry name" value="ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07975">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04056">
        <property type="entry name" value="Ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015919">
        <property type="entry name" value="TFIIH_SSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01047">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="94"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="13">
        <location>
            <begin position="318"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR015919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR015919-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ60894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="422" mass="47107" checksum="E53D74DF8B096768" modified="2017-01-18" version="1">MDIDESDDEFIERDTEHESQTGVANRTRRKAVKSAEIKGANGGYSWEDEYQRSWDIVKEDEAGSLAGIVSGLLESKKKRIIKNVTPFQRGIIRNLILVLDLSSSMLEKDLRPNRHSMAISLAIQFVTEFFDQNPISQLGIIIMRNGIGQLVSQVSGNPQEHIDALKSIKKLEPKGDPSLQNALEMGRGLLMHVASHCTREVLIVDGSLLSLDPGNIHKTVNSLVEEKIRVKIIGLSAQVSICKEICKKTNYGDESCYSVILNEHHFKDLFMDAVIPLPVNKINKSFTLVKMGFPYRVSEEVPSFCSCHSKLTYGGYICPNCKSKICSLPTICPCCNTMLILSTHLARSYHHLIPLKNFMEVPVSDNYSSLNCFGCQIDFPAPSSSQLTSSRYVCTECSNHFCIDCDVFIHEILHNCPGCEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6L9</accession>
    <name>A0A1E3P6L9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ketopantoate reductase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_61091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039724.1">
        <property type="nucleotide sequence ID" value="XM_019183109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6L9"/>
    <dbReference type="STRING" id="683960.A0A1E3P6L9"/>
    <dbReference type="GeneID" id="30200355"/>
    <dbReference type="OrthoDB" id="4068630at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051402">
        <property type="entry name" value="KPR-Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708:SF34">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708">
        <property type="entry name" value="PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="661"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="453"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="671"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="686"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="700"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="718" mass="81309" checksum="4C4C49EE9DDCC5B8" modified="2017-01-18" version="1">MGSGAVNVLIIGDSPTTHFFTWKFLCQRSKFNVFVISEYTKATSKWHSEEGGDQVFDNVVSFISIDKFVQSKSNPVSYDLVLFAIADTAQPATLCQKLKSCLKNDSLILVDASYGVCFEVLILNELPNATVLSILCTADVKLIAKSEYQVLHETQSYNVRVGFSCFSLSGLCEETLLLSKIVESNKFLSLQNSRLDHIMNSLAKIRLVKYLNHTVFARSNLRNSEEGQSFGSYIWFHCIRRIALEVISITFEEFDYQNFNNNKTTAQIVPNLVHELITIAEKSNASDIWNFFRNKKNVIESLLNCYSADELKVMKVAIRSDPIFLHHTDTQYCFAHDLHSPLKMLVYQPLYLSELLTLPNVSIGFLYGIFLERTEVKILKFFRKLKIPSKLNLTDDAVSTATDEIREKNLLALQNKDGVALNELNEENIHDEFYYYDVDQIPTYSENDGDGVDGNTAQNNNNNDNTKTDVNNSDINNDIGNASKNNNNNNNSTQREVKSEQRGKRTVVQDGNSHLSSVHTADGVKLLKSNPDRPRESIQKILEDSSRIPLQLLRPKKERYLVLPIIKWDGSQFLKTDTKLRIVESSRRHVLLEFSKQKIINPQVRPKTPSMNNASYQNYHLDTIQSASNDNTSEILSVTTSRYGHVDSTVNAKGKVEINVTSSSKKRINDSPASKSSISNRNKTRTNRDNLIDELRGLSVSDNQNTENDVETIKSQCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6M3</accession>
    <name>A0A1E3P6M3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ataxin-10 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Copper transport protein 86</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_77650</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play a role in the regulation of cytokinesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ataxin-10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040200.1">
        <property type="nucleotide sequence ID" value="XM_019184590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6M3"/>
    <dbReference type="GeneID" id="30201836"/>
    <dbReference type="OrthoDB" id="379794at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051374">
        <property type="entry name" value="Ataxin-10/CTR86_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019156">
        <property type="entry name" value="Ataxin-10_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13255">
        <property type="entry name" value="ATAXIN-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13255:SF0">
        <property type="entry name" value="ATAXIN-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09759">
        <property type="entry name" value="Atx10homo_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ataxin-10" evidence="7">
        <location>
            <begin position="375"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="479" mass="55281" checksum="BCD43CF574779C6C" modified="2017-01-18" version="1">MVDIGSSLIVLEKVLEAVGSESRSEEPLLDVMSQLTVIVKSSITDLELRHEMAHSHHIWRMIKVDLEQEPEEVNLELKFRLIRGVVLLARNLLSVARDIALEIGIRQAIFKFGLNLQRIQENEGIYNNCLVSAFQCLSNLTVNMKDHEPEVLYELVAVFKSIKTWNEDTQLPVFSYFKNIMDSPDFLHFALSKGEGKPILKELLIQFEKLDLEGELSSIGILIVSIFTKLIVHESFLKFITVYNDPDYTIRYLKVAQTIITSKESWEVFELTVILSWVYELFQKTVPQIQSYFETNSQDEPLFIYQKASSSLDILATLSKYDHSRKFLLSYEGVQNLVGLLGIIQKNVKPKKLKDDQKLEAKKDLKIEYKNFPHIKSFIIEILSSLVYQNFDVQELMRELHGLELVLSNCIIDDNEPFIKERSIVCLRFLLLNNEKNQEFVSKLEAKQAVQDDTLDEAGFEVEIVDGKVKLKQKPNYKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6X2</accession>
    <name>A0A1E3P6X2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_77814</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040373.1">
        <property type="nucleotide sequence ID" value="XM_019184615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6X2"/>
    <dbReference type="STRING" id="683960.A0A1E3P6X2"/>
    <dbReference type="GeneID" id="30201861"/>
    <dbReference type="OrthoDB" id="4065319at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051009">
        <property type="entry name" value="PRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36089">
        <property type="entry name" value="CHITIN SYNTHASE 3 COMPLEX PROTEIN CSI2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36089:SF1">
        <property type="entry name" value="CHITIN SYNTHASE 3 COMPLEX PROTEIN CSI2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="286" mass="30940" checksum="F3C045BAB7484723" modified="2017-01-18" version="1">MPKSLQSKGTSTSSTQSSTSSSTSGSSSGSTSSSMPKMSSSSESFKTTGMPKISATASPNSNSTSSSSSYTVTFTPIVPTASNPEIHIETLPSGTTFIAVGSVLGLVFSVLILTRLIMSCVSSRRARKGNMMQNQQQEFDYDPTLFQEKDLKKHTSRASIYSLGSTSTLNVLGSQASMEQMIAPSGRSLRTALMNNNSRASMFISPTEMLVNQHLYSRSQEDHGYSSYDSPIESPSTAAYTTPSLEPARNDQRRPSRPPSVFMEQLFDDEQSINSYEYDSDAHNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P790</accession>
    <name>A0A1E3P790_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_77895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040460.1">
        <property type="nucleotide sequence ID" value="XM_019184631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P790"/>
    <dbReference type="GeneID" id="30201877"/>
    <dbReference type="OrthoDB" id="10579404at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="277" mass="31484" checksum="B8B87E95165292D6" modified="2017-01-18" version="1">MASNPKPKLKKGYTKSVTSFEGFLHQLKTNHESHQDVQCDGFAHFLKYDLGNLNKLIKGPQVDDTDASSNIVNRNGEAKAVVNGEEVQVYAKKEGKPVKIYAEDGPDGISYTTVPPGNNEEKDNTTSTQVIDSKKEQDVAEYATNDEKLVPLAIPDYKPTREDYMKVIGQVRLEVKKKIINPISKASGYKWEYSKGHFKDEILLLEFSCFQDVTKDMQPSKFTNRHIVEIYKLGECSSSLNVFINVITRQIRISYRHKSHTKLIDIIYSLLSQIYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7E1</accession>
    <name>A0A1E3P7E1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_28079</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISP family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040553.1">
        <property type="nucleotide sequence ID" value="XM_019181447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7E1"/>
    <dbReference type="STRING" id="683960.A0A1E3P7E1"/>
    <dbReference type="GeneID" id="30198693"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015908">
        <property type="term" value="P:fatty acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015918">
        <property type="term" value="P:sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05384">
        <property type="entry name" value="CAP_PRY1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000012">
        <property type="entry name" value="Secreted protein PRY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SCP" evidence="7">
        <location>
            <begin position="3"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="133" mass="14320" checksum="6675B9351D47DD3B" modified="2017-01-18" version="1" fragment="single">DSSFASEILKAHNDKRSQDGASPLSWSKKLEQYAQDYADKYDCSGGLTHSGGPYGENLGLGYSTSGVVDAWYGEKSDYNSNSPSASHFTQVVWKSTTQLGCAKKECGSYWGQYTVCSYDPAGNVAGGYAQNVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7R8</accession>
    <name>A0A1E3P7R8_WICAA</name>
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        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_28165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ61453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_019181455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7R8"/>
    <dbReference type="STRING" id="683960.A0A1E3P7R8"/>
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    <dbReference type="OrthoDB" id="2014058at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="NDUFB8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12840:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12840">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE ASHI SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05821">
        <property type="entry name" value="NDUF_B8"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="19295" checksum="A67F1A3950C733B6" modified="2017-01-18" version="1">MLRINPALRLRPVSILSKRALTTTRFNLQTKIALNEDPTPKIEPQDEIPTSYPDVKPELYYNRDPYGDWDDVQNRRNINEPLHEDEDVLNLWSPEYYQYVSDGEAFKLVSYFFLGVAGIFGVCYTFFYPKRIAVPRNYPHDGLSKALGARNSEEAELLGARVDKTAY</sequence>
</entry>
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    <accession>A0A1E3P7X7</accession>
    <name>A0A1E3P7X7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_61587</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040229.1">
        <property type="nucleotide sequence ID" value="XM_019183203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7X7"/>
    <dbReference type="STRING" id="683960.A0A1E3P7X7"/>
    <dbReference type="GeneID" id="30200449"/>
    <dbReference type="OrthoDB" id="3980997at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031452">
        <property type="entry name" value="Kre1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17056">
        <property type="entry name" value="KRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="220" mass="21966" checksum="C61AE324F46E8820" modified="2017-01-18" version="1">MISIAVVTTTNINGVTVTLTNTVTSTGTGTTNTATTNTATTNTATTNTATTNTATTNTATTNTATTGTGTATTGTGTGTATTGTTTGGAADGEVEGTDTASTGRTTTKLSQGTDTSSVNPTYTYSDATTTYITSSAWTSIWVTVTYSNGGVATVQTTYSQRFKSFYSSVNQPSSGTIGLGSISGSVGVVKSKHLVTQSTNAGIINTISSGLIFVVALLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7Z5</accession>
    <name>A0A1E3P7Z5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_27943</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040740.1">
        <property type="nucleotide sequence ID" value="XM_019181429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7Z5"/>
    <dbReference type="STRING" id="683960.A0A1E3P7Z5"/>
    <dbReference type="GeneID" id="30198675"/>
    <dbReference type="OrthoDB" id="68611at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018823">
        <property type="entry name" value="ArAE_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023244">
        <property type="entry name" value="Brefeldin_A-sensitivity_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052430">
        <property type="entry name" value="IVT-Associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049453">
        <property type="entry name" value="Memb_transporter_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47804">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47804:SF4">
        <property type="entry name" value="AFR661WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10337">
        <property type="entry name" value="ArAE_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13515">
        <property type="entry name" value="FUSC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02047">
        <property type="entry name" value="BREFELDNASP4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="559"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="607"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="640"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="663"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="698"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="Putative ER transporter 6TM N-terminal" evidence="7">
        <location>
            <begin position="17"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Integral membrane bound transporter" evidence="8">
        <location>
            <begin position="588"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="993"/>
            <end position="1018"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1107" mass="124972" checksum="CC75533DFF7A9F89" modified="2017-01-18" version="1">MQQITSSLLRSISKKLPNRILAQRIFKCVFNSTIALIFCLIPKVVDVLGDEPAMLPLISVMVHPGRRFGTMIEAVIFCISGLLLGNSYALLGRFLAQKALTPEKLHLVDKLQKVENYQNYRTALAILAVFEIIMLFFHGWMRSISHKFFAMVFPLFLVVHFTFMDNLKTDAGTIAKSFTIPFFLGIAISLACNLLIFPEFGSSYLGRSMIITLNELHSTIDSTVQFFISVGNDTSLFKVECPSLAQLTKKKQSLRAKLNSTQAVLQECMYEISYSYLSPAQLRNIIEVLKSQLSYVSALINASQLEFTLLNDNSTTEGENDNKLLITVLERARIPIFELQKVISDSLYVIKVGIAKSYDVDFSSISKSPSIELQDFDLESEDFDARINTLARAMAEFDITFRKELDNLNTEYEDYLSPNDDMFLLSSFLMNLKETANAVCNIMKETKLIYEYRLTQERKGFFGRKVWFSFLTSSDQLKGWFSANSNDLANANETASLNGDTQSKIYLGDDDRPRRKSMSTENEFFENVAAKRKQTIHHNYFDKIIVNSLAFYEKFKPHFRFGIQITVGLMVASFPMFIPKSREWYVDIRGTWIGFVCILVLEPQVGSTFFVFFLRGVGVISGAAWGYLSYVAAINQTNPYLETIVTIFGAIPGYYYFLGTPYVKAAIIGIISIYIVLLSSALPSEIGGSILQNFGKRCLAMIYGGAVALICQLIIFPIKARDELIYEVSDAIGMIAKLQLIYAVGLNGEDSKLSMTEERYENYKKLSATAKSALTRADAYRGSARKEPRLKGNYQDIERVFYEIIFILREVLDRMDNIVLLRKVYGSAIIEEFNSTVFPYRRQVTASINNNLRAVQCALMTKAPLPQYLPSSKIAQQRLIKKVTEVVQEKYPIIKKENNESSDSEGDSQIFVEFRGHGHRPDSSHREHLLKEKFLSWNASSSATEEVIDYLEELVELIKVLVGVHEFKYGFLSRSLYSDYAAKAARGYKDMVTGHEDEDKGNATTPVEDEDDINDSASSVEEYDVNPIALVNTGVSSKSRKSVDSNATKLSNGFRKRVYSIGSWVRGDLTRTKSLGQYTDGEIEGDEDNELPLSLMRVVSKKSTIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P883</accession>
    <name>A0A1E3P883_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_52559</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Golgi apparatus</location>
            <location evidence="11">trans-Golgi network membrane</location>
            <topology evidence="11">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040631.1">
        <property type="nucleotide sequence ID" value="XM_019182691.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P883"/>
    <dbReference type="GeneID" id="30199937"/>
    <dbReference type="OrthoDB" id="242910at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120095">
        <property type="term" value="C:vacuole-isolation membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051365">
        <property type="term" value="P:cellular response to potassium ion starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000425">
        <property type="term" value="P:pexophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13980">
        <property type="entry name" value="STKc_Vps15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000497">
        <property type="entry name" value="Phosphoinositide 3-kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045162">
        <property type="entry name" value="Vps15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583:SF0">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="27"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="970"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1297"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1326"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1326"/>
            <end position="1339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ61424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1392" mass="158653" checksum="9A285F05F476D280" modified="2017-01-18" version="1">MGSHLSLMAPTAPSIAVSAYVDVLDQIQYLQELGTSRFLKTVKANDTDGSIVIKVFIKPDDNLELSQWRKVLDEQKAKLIDIPSVLPYLKIIETDRAGYLIRQFIKTNIYDRISTRPFLEDVEKKWMIFQLLNTLNECHEQGITHGDIKTENILVTTWNWILLSDFAPFKPVYLPEDNPGSFSFFFDTSQRRTCYLAPERFLNNADYTPELNNDAKITNEMDIFSLGCAIAEIYLEGTPIFSLAQLYKYKRGEFIPDLAGIDDVHIRELVESMISLNPAHRLSAKEYLEKYRRLAFPENFYTFIYEYFKSFNEDIVPFHSTGRVYECDSRINRLYNDFDKISYFLGYKYDVTEEDEMDNDEKIRNGSLVPIRLSLPGIPKNYSLKSTSKVVGGTDDSALIILSFIFTSMRNVRQEASKIQALELILALSERIHDEAKLDRVLPYVIVLFTDSSINVQAAALKSLTQLLLLVDAIGPVNVLLFSEYIIPRLSNLLSNASPYLRMVFASCLPYLAQTSLRFHDMATILKTTNDGFNTAGMFDVSKESLISDFETFAISMLTDPEANVKVALLRNILPLCSFFGKEKTNDLILSHLITYLNDKSSFLRISFVEAVVGLSIYVGTTSLEHYILPLLVQTLTDPEELVVIKVLQIFQDLSKLGLIKKDYIWDLLSTVSKLLLHPNEWIKQSTLSLIITIADTLTLTDLYCMLYPIIRPYIDYDVTDFSWDTLFSVCKRSVSRSVYNLACTWSLRAENTLFWQQVKSPHTDAFGNRGLDFLSKPIPGMSNSKVSAGNSIIFSNMEVPLSNEDKGWVDRLMSSGLNGQELWKIADLREYIYRVSRLTTRTGSRDFEERNGVEIQQLGVLPRNVFFDSKLHSEVLVTPPIDEISTNSPIPSIDDRLTIDAAPDPNIISSTVTNSYTGNDPYVVRFLNNVKIEPSLEEFPEFNQPIPQAQFNVSISRSWSPKGDLISHLKEHKSGISTIDVSPDHNFFITGDDDGQLKVWDTMRLERNVTQSSALSVDLESPIVKIKFLSTHYSFAVALKDGSIKVIKINFKKSKRQHTVMDSFTVIRETKLENEYATDLLFGQKDQKHVLYLVTSASSIIAMDIRVMETIFKLEENPSHGNLTCFTVSRDNSWLVIGTSRGILSLWDLRFRLHLKSWKFKSGYPIKKVISCSDDYHLNRKKGRYVTVIGGTGDSDATIWDISTGICREVFTRSAENNNLDKFNLVELNDGKAVESEILKGFSNLELDNIQKDYSFTALQLIEKHIDHRRKFWILTASPAFEMVLWNVHHPEDSRMLSNNSTNNQQGKPTYSTSQINPNLRIITERYPTEPETGSKDAKSKKKRGKGSLLTEAQEELMKRHHDIITDFVLVLKPYEMIVSVDRSGVINVYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P894</accession>
    <name>A0A1E3P894_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">JAB1/MPN/MOV34 metalloenzyme domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_59714</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M67A family. CSN6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040838.1">
        <property type="nucleotide sequence ID" value="XM_019182861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P894"/>
    <dbReference type="STRING" id="683960.A0A1E3P894"/>
    <dbReference type="GeneID" id="30200107"/>
    <dbReference type="OrthoDB" id="1378at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024969">
        <property type="entry name" value="EIF3F/CSN6-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540:SF8">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13012">
        <property type="entry name" value="MitMem_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JAB1/MPN/MOV34 metalloenzyme" evidence="2">
        <location>
            <begin position="5"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="282" mass="32169" checksum="F84FECE3D6894225" modified="2017-01-18" version="1">MSAKRVALHPLVMLNLSDHYIRHQQQSTAVANGRLLGVLLGNEGKDGSLQLINSFEFAFQDDGVPDLSYLQTRLEQQSVILPNEYLVGVYEIKQKHELKPSDEILQLHDLLKEYNVDLVTLVFNPELSLKKFTNQKFVKIYDNQLNDLKYSVDTKEAEKVAVDTVLDVKDVENEDELNKATDQIESQSFILKTLHSKIELILEYLTKTDPASTPGYYDILRQVNSFISKVQSKQNDDIKTKLIDLETDYNMLISLGTMNDTIKGLDSINLDLKKYDSQPVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8J5</accession>
    <name>A0A1E3P8J5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Peroxisome assembly protein 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14 16">Peroxin-12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">WICANDRAFT_27360</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the PEX2-PEX10-PEX12 retrotranslocation channel, composed of PEX2, PEX10 and PEX12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040837.1">
        <property type="nucleotide sequence ID" value="XM_019181365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8J5"/>
    <dbReference type="STRING" id="683960.A0A1E3P8J5"/>
    <dbReference type="GeneID" id="30198611"/>
    <dbReference type="OrthoDB" id="107372at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016562">
        <property type="term" value="P:protein import into peroxisome matrix, receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017375">
        <property type="entry name" value="PEX12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888:SF0">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12 PEROXIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038074">
        <property type="entry name" value="Peroxisome_assembly_p12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 16" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="318"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR038074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ61630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="386" mass="44932" checksum="A44C6B41D22FF066" modified="2017-01-18" version="1">MDYYSSLDSRSLDSTPTIFEIISSNELEQLLSPSLRYIIVHYAQKYPNLLLKFAMKFDELNLVFRGLVEYHYLSKWNSSFTENFYGLKRQTLNKINIKDKNHKIFEMIETKKRLSNWQIYASLINLVGGAYVNEKLDVIYEKLYGKVLLKTLKPTTLTNKLKYYYVKVYPYLLSIIKLLNVVFQILYLSGRTKSPSFIDYLLKIEYARLSQFDYDLHDKKSSTTTKPETSSTSTRTRPPSFGQHLNRMLSVYYKPLKSLMMKLSTTVFPLAIFLLKFLEWWNSSEFASKISKNQQNLIDKDIPPPNSSKIPITKTDICPICSNQITNPAVIETGHVFCYPCIMRYLMESDPVKGGRCPITGRRLIGCRYSNAAKEWKVDGVRRLMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8M5</accession>
    <name>A0A1E3P8M5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mitochondrial fission process protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Mitochondrial 18 kDa protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_25925</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MTFP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040973.1">
        <property type="nucleotide sequence ID" value="XM_019181206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8M5"/>
    <dbReference type="GeneID" id="30198452"/>
    <dbReference type="OrthoDB" id="424969at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019560">
        <property type="entry name" value="Mitochondrial_18_kDa_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11001">
        <property type="entry name" value="MITOCHONDRIAL FISSION PROCESS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11001:SF2">
        <property type="entry name" value="MITOCHONDRIAL FISSION PROCESS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10558">
        <property type="entry name" value="MTP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00029631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="223" mass="24907" checksum="43B34EB0F7DA6215" modified="2017-01-18" version="1">MSKSNDITTSIQEISESTKPGSLDNSDSVDSNVRYAAYANRLRTILLASHRYVAYTSDIGESFRPAVHPFFVRACYGISWTYVLGDVSYEAWKAKLRKEGRYTPGLKPWNSNISAKGDPTAATLAELNALPDWRLVALERGLFQSVASMGLPAFTIHTTVKYATKYAEKNIKNAVLRTWGPIGLGLSIVPALPYLFDKPVEHGLGYVFQNLEQYLHNKEKKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8R5</accession>
    <name>A0A1E3P8R5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pre-mRNA-splicing factor CLF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_59877</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in pre-mRNA splicing and cell cycle progression. Required for the spliceosome assembly and initiation of the DNA replication.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041006.1">
        <property type="nucleotide sequence ID" value="XM_019182892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8R5"/>
    <dbReference type="STRING" id="683960.A0A1E3P8R5"/>
    <dbReference type="GeneID" id="30200138"/>
    <dbReference type="OrthoDB" id="541719at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071008">
        <property type="term" value="C:U2-type post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000354">
        <property type="term" value="P:cis assembly of pre-catalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055433">
        <property type="entry name" value="HAT_Syf1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055430">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF3">
        <property type="entry name" value="CROOKED NECK-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23231">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23233">
        <property type="entry name" value="HAT_Syf1_CNRKL1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats" evidence="12">
        <location>
            <begin position="51"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats" evidence="11">
        <location>
            <begin position="347"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="29"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ61799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="664" mass="80037" checksum="FCD2B9F77A86590F" modified="2017-01-18" version="1">MEKNRTPAEFQISAEQILLEAYERKDEPLNKTNVKIADLEELHEAQRRQRQEYEDALRRNRLDFGQWMRYAQYEVEQKDLRRARSIFERALEINSHHVPLWIRYIDTELKSRNINHARNLFDRVTTLLPRVDKLWFRYAQTEETLGNVIGTRNVFKRWMKWQPDTPAWDAYINFEKRYDEFDNVRKVFNQFVYVHPYAETWIKWARFEDEFGTADNVREVYSASIDSLLNEKLIVNFAKWEGQQKEWERARAIYRFGVSKFPNSILINDQLSQFEKQYGDKDGIESSILLKRKKRYEDELDSDPLDYDTWWAYLSLLENYPLEVQREAYEKAVANVPTSVEKNSWKRYVLIWIRYATLEELNEEIEKTREIYKRLTKVIPNKKFTFSKVWILYSNFEIRQSHLQQARKILGFAIGSYPKPKTFKHYIQLEIKLKEFDRVRKIYEKYVETFADHADVWIEYAELESNLNDIDRARGIFEIGLEQLASSKSVSELWYRYVDLEVEQREYERGRYLFERFLETDKNSTTIWIKYALYELGVPTQDQIAEYEKKVEEAGDDELEFEFDISESSKSKTRDVFEKALTHFKSNNLKDERIVILEAFKKFEQVHGDDSSIAKIEKRQPTVVKKVKELEDGSTREYFDYVFPDDIKFSKFLENAKKWAKQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8R9</accession>
    <name>A0A1E3P8R9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_61826</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040474.1">
        <property type="nucleotide sequence ID" value="XM_019183252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8R9"/>
    <dbReference type="GeneID" id="30200498"/>
    <dbReference type="OrthoDB" id="3980425at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="132" mass="15933" checksum="0CE703A58F106D42" modified="2017-01-18" version="1">MVEYTKEEQEKLVQRFEIQRDERRAGLVNEIRKLCEIFESKIQRRVHKVPKKFAELKIKDVLNVERDHKIKIYQLISDIEAYKKTNNRKINDDEQMRLEIAQLRRKKADNLRSLQKRKESIATTGRVSKPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8T2</accession>
    <name>A0A1E3P8T2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Profilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_83516</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the profilin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040569.1">
        <property type="nucleotide sequence ID" value="XM_019185558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8T2"/>
    <dbReference type="STRING" id="683960.A0A1E3P8T2"/>
    <dbReference type="GeneID" id="30202804"/>
    <dbReference type="OrthoDB" id="421374at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070064">
        <property type="term" value="F:proline-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140311">
        <property type="term" value="F:protein sequestering activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090338">
        <property type="term" value="P:positive regulation of formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00148">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.30:FF:000001">
        <property type="entry name" value="Profilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048278">
        <property type="entry name" value="PFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005455">
        <property type="entry name" value="PFN_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036140">
        <property type="entry name" value="PFN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027310">
        <property type="entry name" value="Profilin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11604">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11604:SF0">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00235">
        <property type="entry name" value="Profilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00392">
        <property type="entry name" value="PROFILIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01640">
        <property type="entry name" value="PROFILINPLNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00392">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55770">
        <property type="entry name" value="Profilin (actin-binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00414">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="126" mass="13718" checksum="370CDF19B02A92ED" modified="2017-01-18" version="1">MSWQAYTDNLISTGKIDKAAIYSRAGDSVWAQSNNLTLSGPEISELARGYDDPSGLQANGLHLQGQKYFLIRNDERSIYGKHDAEGVVTVRTKQTILIGHYPAGVQAGEATTIVEKLADYLISVGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P931</accession>
    <name>A0A1E3P931_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">DASH complex subunit DAD3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Outer kinetochore protein DAD3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_20322</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DAD3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040931.1">
        <property type="nucleotide sequence ID" value="XM_019180865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P931"/>
    <dbReference type="STRING" id="683960.A0A1E3P931"/>
    <dbReference type="GeneID" id="30198111"/>
    <dbReference type="OrthoDB" id="2443965at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013965">
        <property type="entry name" value="DASH_Dad3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28017">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28017:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08656">
        <property type="entry name" value="DASH_Dad3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="15" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ61724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="68" mass="7869" checksum="470A8EE717BA1C4F" modified="2017-01-18" version="1" fragment="single">QLTELETKVLIQYQNLAKRLNHLAQVINKLINQPSTKLLENLQLIESKISLVFTLFKGAVYSLFLQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9D5</accession>
    <name>A0A1E3P9D5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DASH complex subunit DUO1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Outer kinetochore protein DUO1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">WICANDRAFT_87146</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DUO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041232.1">
        <property type="nucleotide sequence ID" value="XM_019186096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9D5"/>
    <dbReference type="STRING" id="683960.A0A1E3P9D5"/>
    <dbReference type="GeneID" id="30203342"/>
    <dbReference type="OrthoDB" id="5599235at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013960">
        <property type="entry name" value="DASH_Duo1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28216">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DUO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28216:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DUO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08651">
        <property type="entry name" value="DASH_Duo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="70"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="70"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="108"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ62025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="142" mass="16302" checksum="5DC0BDDB55C31276" modified="2017-01-18" version="1">MDATSSHIDTVISTTDSTDKLLDLWIKILSQTSYTTALLNDKSWKGVTQHEEEYASQLRKFEELNKKYTAEKQRREQEKESQRQKRQALEQRAKEREDALRRRVYGNSSTSTRGSSVTRRGTTSSRSASVRGRGTTKGTVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9I1</accession>
    <name>A0A1E3P9I1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4 14">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_76250</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4 14"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041276.1">
        <property type="nucleotide sequence ID" value="XM_019184348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9I1"/>
    <dbReference type="STRING" id="683960.A0A1E3P9I1"/>
    <dbReference type="GeneID" id="30201594"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031502">
        <property type="term" value="C:dolichyl-phosphate-mannose-protein mannosyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23284">
        <property type="entry name" value="beta-trefoil_MIR_PMT2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000012">
        <property type="entry name" value="Protein O-mannosyl-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF52">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
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    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="207"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="236"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="622"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="687"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="718"/>
            <end position="736"/>
        </location>
    </feature>
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        <location>
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    </feature>
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        <location>
            <begin position="482"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
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            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ62069.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="758" mass="87117" checksum="7FD6D317EABCCDF5" modified="2017-01-18" version="1">MSSKVTGSSKNESEVRARKVVIDEVTNDEIVTDQIETDEIIQDSDEKKSRSTKCSQLCQKLLKIESVLGPLLFTLLALYVRFYKIGIADKVVWDEAHFGKFGSYYIKHEFYHDVHPPLGKMLIGLSEWIAGFDGDFEFKSGTKYPENLDYKTMRYFNALFSAACVPVTYFAAKTLDLSLITVYFIALMVTLEASYIALGKFILLDSMLLFFTITTFFAFAKMHSLRRAGQEFTKSWYGWMVFSGLSIGAVCSVKWVGLFITILVGLYTIQDLFEKLFEKDFEIKKYAKHWSVRIATLIFIPFLVYLISFKVHFHLLSKSGTGDASTSSLFQANLENNNIKKSPRDIVYGSEVTIRSHGMSPNLLHSHVQIYPEGSNQQQITAYGHSDGNNNWVFKYSRRSGQTLDENDDTLVNVKDGDIIRLVHKFTGVNLHSHTIPAHVTKKHWEVGGYGNEQVGDSKDDWVVEIVEQLHSANKSYPKEDGSILHPLSTNFRLRHADLGCYLATTGASYPTWGFKQSEIVCKNSWSKYDKSTWWNVEDHMNGKLTIDENYVAPKSNFWSDFMLINVAMASSNNALVPDEDKFDALASKAWEWPTLHVGLRMCGWGPRDARYFLIGNPFNTWLSSVSLIVFIYLILHSVIKYQRQTLEWTEDEYWRFIVAGVYPLIGWFLHYMPFVIMGRVTYVHHYVPALYFAIFVLGYLVEFFIGKTKPIIKYPIYAILFGVLIFTYFFFAPFYHGMVGSNKDYEYLQLFSSWRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9I7</accession>
    <name>A0A1E3P9I7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Meiotic nuclear division protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_38060</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for proper homologous chromosome pairing and efficient cross-over and intragenic recombination during meiosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the MND1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019182530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9I7"/>
    <dbReference type="STRING" id="683960.A0A1E3P9I7"/>
    <dbReference type="GeneID" id="30199776"/>
    <dbReference type="OrthoDB" id="9978204at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Mnd1"/>
    </dbReference>
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        <property type="entry name" value="Mnd1_HTH"/>
    </dbReference>
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        <property type="entry name" value="Mnd1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mnd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mnd1 HTH" evidence="7">
        <location>
            <begin position="2"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR026991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="189" mass="21803" checksum="3AAF702C68BF80D1" modified="2017-01-18" version="1">MFYNIKEVEAQGSKFTGVHSMQIKDLLQSLVDDGLVNAEKCGVTTLYWCFEYDKHKKMKILVEKSAEKLGVVNNDIKKITEKIEIEKAKRTKGNSQEMLKQIDSLRKQKQSLAEKMSSSAFDMDRVDSIKKELQDEISNGEICTDNIESLIYYFQKNNGINRGVFKQELNIPEEFKDFPNLDEVFAKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9L5</accession>
    <name>A0A1E3P9L5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_60127</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MUB1/samB family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041265.1">
        <property type="nucleotide sequence ID" value="XM_019182941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9L5"/>
    <dbReference type="GeneID" id="30200187"/>
    <dbReference type="OrthoDB" id="5594178at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990304">
        <property type="term" value="C:MUB1-RAD6-UBR2 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.140.2220:FF:000003">
        <property type="entry name" value="MYND-type zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051664">
        <property type="entry name" value="MYND-type_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47442">
        <property type="entry name" value="MYND-TYPE ZINC FINGER PROTEIN MUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47442:SF1">
        <property type="entry name" value="MYND-TYPE ZINC FINGER PROTEIN MUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="9">
        <location>
            <begin position="607"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="343"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="652"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="351"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="363"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="373"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="652"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="681"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ62058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="690" mass="78614" checksum="8513F76B9045764A" modified="2017-01-18" version="1">MRDSNYKSISLNRAAVSISTNLYDRRALDSTSDRPLVNSLNHLTFLTSSSAKVRETLSTDGGLERLVGILHECKKKPDNVVGEKKKTIAQEKNDAIIAWKWTLAFQCLVLIGTRGTESIRKRVVEAGIIPIIATVLDNYLLIVRNFDNNIDNNLNDNFLNLVNLQQKQSILQQQQDSLSKENYDDYDAEIKNYLNSNSELFNYLHEDYNTPPSTSSSSSQEEANNTTNSPIQVLRNINNEINIQQNLQDINQTINSPRFFLNGILIPKEDDVVWSLQLLAFISKYSNLKDFLQFTNLVDPLSLRSLLNKNNSSNQQNCSIPPPLENDDLDNELEYELISNLSSDENDKNNHQNQQQQQQQQQPSNTQSTIEDSSQSQPQTQRPSSQSSTSQILIQNNNESNDNIITDNDELINSNNEKPDLSTLVNLYSQSNSINNSNKNLNNLNNINNNNVNDQLIEKKQSCFTKSLLNSFIKFAHCCEKESPPPSPHPSNEAKTLSLIELTIKLNKFYQRKHSLNKLTKIQSNIKKREQYQSKWNYNSYFQKNPSSSSDIDSSLKITHHLNIFPLVEKFTIKSINSPDMCYWSGVIMRNSCRKDESRGGVRQCASFDCGKWEEFPRQFAKCRRCKRTKYCSKNCQLKAWVYHKHWCVDSNSSSSGMSSGRIQNSQRQEENEGAGGAGATTQLNENLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9N4</accession>
    <name>A0A1E3P9N4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_27098</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041285.1">
        <property type="nucleotide sequence ID" value="XM_019181337.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P9N4"/>
    <dbReference type="GeneID" id="30198583"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF119">
        <property type="entry name" value="ROA1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="492"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="516"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="579"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="629"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1042"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1075"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1113"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1151"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1182"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1271"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="72"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="697"/>
            <end position="950"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ62078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1309" mass="146834" checksum="542CC9BD28257436" modified="2017-01-18" version="1">MTSKWHPTVTNSYSSSHDIELESNEIDQSKDQPQLSFVNIDPVPVKVRDLSISARLDNPFQRLNPFHRFRNKKNKDDLENANIKTILDTTSFDLPSGSLMAIIGGSGSGKTTLLNVLANRSSSSTLFRKGDISFGGKSLNHIRNAYVIQQDILSPNLTARETLVYATELRLPDSTTKLERLRLVEEVILELGLKDCADTIVGDSTHKGLSGGEKRRLSIGIQILSNPSLLFLDEPTTGLDAHSAFLLIKTLKQLANRGRTIIISIHQPRSDIFFLFDYLCILSQGKTVYSYKVDKVLEYFGQLGYTVPENVNPADYLIDITAVDIRTTQAEQESSKRLVKFVNNWQNYEKTIINDNIEMEKGIPMASSVNPANSQHKAPFWRELTVLTRRTFLLSIRDSVTLLSMVCEALFMGIICGWVFYKPKHDLSGIRTMTGAAYSANALQGYLMLLFEVYRLTSVDIKLYDRERSDNCVSIVGFLLSRRIAKLFTEDIIVPLFFSLTTYFMFGFIYTAKKFFIYFATIFLIHETSMTGAMFSVSLSRNFANASLFSNLNYTLQSMACGYFVNAKTMPVYVRWTKYIAYVWYGFGALVSNQFTGFIGSCPYENPQDCASYQGKYIIHNLGYWENWITVPIVVIMCWAIGFYLVAGVILKFNKVDVALSKQVKSSTPEEKQDEELEQLEHVREEVNTPEKLNIDVNLLNLCLGVRIKKGRTSKEKIILDNISATFKAGKVNAIMGPSGSGKSSLLNLVSGRLSSDLINRYTSSGEIFFNNYIVTTKMVNSICSYVSQDDDHLLATLSVRETLTVAADLRLGQLPQDKKFEIVEDIINKLGLKSCANTLIGSEFIKGISGGEKRRVSIAIQLLNNPKILLLDEPTSGLDSFTASSILEILQKLAYEGKTIILTIHQPRSDLFQKFGQVLLLAKGGKVGYNGEQLKMVDYFASLGYPCPKLTNSADHVLDLISVNNQTLHKEKITKERVEKLLNNWSGMNVSTSPQDVRTINESEFPKIFGSFIREPAKFHVAFYACVKQLGITTLRNQQVFIARISQVAGMGVIIALFFAPLKNDYIGVSNRLGLVQEVTSLYFCGMLNNLSSYPDQRNYFYHEYDDRVYGILPFFLSYMIVEIPFEIISSFIFAAFLGPVVGLPRTPEMFFAIAYVSFLITFCGESLGIITNTIFHHAGFAVNVVSIILSIGTFMAGIMSLQMDSVLKGINWISPLKYGMSILLNMAFSDDVTFKCDEASSRDVNGDCVFNNGGDVLRQYALKAPYKKFFGVLLVVAFIYRFVIAYGILRLKLLKINLGVFNSTAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9S3</accession>
    <name>A0A1E3P9S3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Guanine nucleotide-binding protein alpha-1 subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">GP1-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_26788</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041325.1">
        <property type="nucleotide sequence ID" value="XM_019181300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9S3"/>
    <dbReference type="STRING" id="683960.A0A1E3P9S3"/>
    <dbReference type="GeneID" id="30198546"/>
    <dbReference type="OrthoDB" id="5817230at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
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        <property type="term" value="P:regulation of carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
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    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-5 SUBUNIT"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
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    </dbReference>
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        <property type="entry name" value="G_ALPHA"/>
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    </dbReference>
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    <keyword evidence="8 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="2" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="50"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
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        <location>
            <begin position="281"/>
            <end position="287"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
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        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="306"/>
            <end position="310"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="375"/>
            <end position="378"/>
        </location>
        <ligand>
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        </ligand>
    </feature>
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        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ62118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="459" mass="52370" checksum="DAFB33F643033436" modified="2017-01-18" version="1">MGCAASTPVQEEDDPLLQSRKANAAIEKRLMMEKQREKNEVKLLLLGAGESGKSTVLKQMKVLHQNGFTHQERQQYAQVIWADAIQSMRILIIQARKLGISLDSDLPNSELSHFKTVILRAKVLDHIDTGIAGGSNFLNDYVLKYSERSEAKRRIQSTGRTGSVWDSIEQKQQQEQQQLLGGGDNGDKVTQLEEITQGLNDTSIRPGVSTINSNVLNNQSENFTRDLIAESIKKLWYEDKGIQQCFIRSNEFQLEGSASYYFDNIDNFANPNYICTNEDILKGRIKTTGITETNFNIGSSKFKVLDAGGQRSERRKWIHCFQDITAVLFVLAVSEYDQMLFEDERVNRMHEAMMLFDSLCNSKWFINTPFILFLNKIDLFEEKIKRSSIRKYFPDYRGKLGDVNSGIKYFEDIFLGLNRTNKPIYVHRTCATDTQSMKFVLTAVTDMIVQSNLKKSGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9V8</accession>
    <name>A0A1E3P9V8_WICAA</name>
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        <recommendedName>
            <fullName evidence="5">LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_18293</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="ODQ62199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019180778.1"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
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        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="5">
        <location>
            <begin position="47"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="5">
        <location>
            <begin position="163"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="222" mass="26712" checksum="8625368D1C0E28F9" modified="2017-01-18" version="1" fragment="single">RYFKEGSLLELPHLSHYDLRFYYNRIEPIDRLAILHRIARSWFRFTNRLELKTWIAHGSLLGYYFNGLIMPWDDDLDVQVTASSFEKLMEYNGTLVIDYNDLNLGKYLIDINPWYGVRSKESDNKIDARFIDVESGLYIDITTLSHDYIDKNDVQNENLIFCKDYHVYKIDELSPLIPTIFEGEVGYVPNNIQNLLLREYKRKSLYLFEYRGFQFDKFRRIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA92</accession>
    <name>A0A1E3PA92_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein YTP1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_82307</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041427.1">
        <property type="nucleotide sequence ID" value="XM_019185365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA92"/>
    <dbReference type="STRING" id="683960.A0A1E3PA92"/>
    <dbReference type="GeneID" id="30202611"/>
    <dbReference type="OrthoDB" id="4137487at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018827">
        <property type="entry name" value="YTP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31685">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_6G12730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31685:SF2">
        <property type="entry name" value="PROTEIN YTP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10355">
        <property type="entry name" value="Ytp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="288"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="361"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Protein YTP1-like C-terminal" evidence="3">
        <location>
            <begin position="145"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="399"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="427" mass="48247" checksum="2E19F30A1AD920C5" modified="2017-01-18" version="1">MDMDDSEEFTRPNITNAGSKTFHWLMTLALLLILPSISTTLVFAEKVHTAFFLQIVSLVYAVIEALFLRFPDGDGHEDRTSRGTAWFLLFLNALTVFIGSMNSGANWFMNKNGETKKEFHWIAKLGEKSVRIIHRTLSVLLTLTGWVKSCLAPVSMFGFCREIHTGQCLAHGIMGTAFIGYGLVYALILVIPRIRETKHRNSQDFYDSLVMTVWGIVNTFTEHRWGREGWGHGDYQHTAMGIIWWSGGLLGLYLGRGGRRTWIPSLLLIYTGWAMSEHNQHLAISTKVHYMFGLSLMLGGAIRIAEISFLLKDARSIPGKIMSFQYLPSFCLIESGVLFMAANEEQLRLVLRLEADHSSYIMVVTSAAFLIYLWFLLLLELFLNLTSSNRIDLSKYASVSGSEPNPDAEGNFATDFELDNFDHGEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAA2</accession>
    <name>A0A1E3PAA2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_12249</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AFG1 ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041361.1">
        <property type="nucleotide sequence ID" value="XM_019180526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAA2"/>
    <dbReference type="GeneID" id="30197772"/>
    <dbReference type="OrthoDB" id="548867at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002222">
        <property type="entry name" value="AFG1-family ATPase, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005654">
        <property type="entry name" value="ATPase_AFG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040713">
        <property type="entry name" value="ZapE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169:SF2">
        <property type="entry name" value="AFG1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169">
        <property type="entry name" value="ATPASE N2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03969">
        <property type="entry name" value="AFG1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="586" mass="68172" checksum="CE5D6A72DB6C823D" modified="2017-01-18" version="1" fragment="single">SISVTDPLVIYQNYVSRGVLKPDAAQLRAAVEFQKLYYRVKDYVPPEDVQIRIRQLVREIEARHANNNTNYTPSWYKPSVTKSRDLIKVLTDEEELTNFPSPHGFLLNGEVGCGKSMLMDIFANSLPHESKCRWHYSNFMLWVYNGIHLIYQRRAQNSNHTRHSMISLENEFILFEIAQKMIRKNTVLLLDEFMLPDLAAAKIVKLLFTYFFRLGGVLVATSNRLPEDLYATDFRKKEFRSFLRVLEARCYSLDMNSENDYRAILMEESNEKNCKSYLISKDSLTHEQDWLDLLSTEIDSFDLSKGDEQKITVYGRELTIPWHNNGVVKFDFNDICKGLYGPGDYISLASNYHTFIIDNVPVLKVSMKNEARRFITLLDALYESKCKLFLRTQVSPDSLFFPDTSSKEEQEEANRIKVQDEEMFSKTQIALTAPYRPNVSYYDDEKIVYNETETAKVVKNFDLSSTNFTDRKAFTGEDEKFAYKRAVSRMKEMTGSLHWRLEGKWTPVDKSMRPWERLKSEQLNDANIEFEAMDNEHNVVTGRVVHNASGVLAPVFNVVHFWSMGLWGKGNRLKDDIARKWIKGNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAE7</accession>
    <name>A0A1E3PAE7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Chorismate synthase</fullName>
            <ecNumber evidence="4 8">4.2.3.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_25655</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21020"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="EC" id="4.2.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>FMNH2</name>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
        </cofactor>
        <text evidence="8">Reduced FMN (FMNH(2)).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the chorismate synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.5" evidence="4 8"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041592.1">
        <property type="nucleotide sequence ID" value="XM_019181169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAE7"/>
    <dbReference type="STRING" id="683960.A0A1E3PAE7"/>
    <dbReference type="GeneID" id="30198415"/>
    <dbReference type="OrthoDB" id="1721239at2759"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004107">
        <property type="term" value="F:chorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042602">
        <property type="term" value="F:riboflavin reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07304">
        <property type="entry name" value="Chorismate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.150.10:FF:000004">
        <property type="entry name" value="Chorismate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.150.10">
        <property type="entry name" value="Chorismate synthase AroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00300">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000453">
        <property type="entry name" value="Chorismate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035904">
        <property type="entry name" value="Chorismate_synth_AroC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020541">
        <property type="entry name" value="Chorismate_synthase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00033">
        <property type="entry name" value="aroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003793">
        <property type="entry name" value="PRK05382.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085">
        <property type="entry name" value="CHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085:SF0">
        <property type="entry name" value="CHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01264">
        <property type="entry name" value="Chorismate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001456">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103263">
        <property type="entry name" value="Chorismate synthase, AroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00787">
        <property type="entry name" value="CHORISMATE_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00788">
        <property type="entry name" value="CHORISMATE_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00789">
        <property type="entry name" value="CHORISMATE_SYNTHASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ62385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="377" mass="40712" checksum="809988DEFAD36374" modified="2017-01-18" version="1">MSSFGTLFKVTTYGESHCKSVGCIVDGVPPGLELTEADIQPQLTRRRPGQSKLSTPRNEKDRVFIQSGTEYGKTLGTSIGMLVANEDQRPHDYSETDLYPRPSHADFTYLSKYGIKASSGGGRSSARETIGRVAAGAIAEKFLQKANNVEIVAFVSQIGEVKMTRDVTDPKFVELLNTVTREQVDDAGPVRCPDPSVAQAMVEEIEKYRDAKDSIGGVVTCVIRNAPIGLGEPVFDKLEAQLAHAMLSLPATKGFEIGSGFEGVQIPGSKHNDPFYLEEETGRFRTKTNNSGGIQGGISNGENIYFSVAFKSAATISKEQPTATYEGKKGVLAARGRHDPSVTPRAIPIVEAMAALVLADSLLVQKSREYAHNIVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAG0</accession>
    <name>A0A1E3PAG0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Processing of GAS1 and ALP protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_76579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041616.1">
        <property type="nucleotide sequence ID" value="XM_019184409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAG0"/>
    <dbReference type="STRING" id="683960.A0A1E3PAG0"/>
    <dbReference type="GeneID" id="30201655"/>
    <dbReference type="OrthoDB" id="4227028at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011431">
        <property type="entry name" value="Trafficking_Pga2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28199">
        <property type="entry name" value="PROCESSING OF GAS1 AND ALP PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28199:SF1">
        <property type="entry name" value="PROCESSING OF GAS1 AND ALP PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07543">
        <property type="entry name" value="PGA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF022909">
        <property type="entry name" value="UCP022909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="14739" checksum="A8E694178B5F9A50" modified="2017-01-18" version="1">MSEIIDKIVPYIPESLKEIDIHKAIRLTVIVGGYILIRNVAQKYLANRQLENQLKQDTERKEQERIEDLVDDPNAKASGLDDGSWGWGKKTRRNVKRQEKILQEQIENLQNGQALDEDKDIEDLLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAQ6</accession>
    <name>A0A1E3PAQ6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-chaperonin molecular chaperone ATPase</fullName>
            <ecNumber evidence="2">3.6.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Hsp70 chaperone Ssb</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_86996</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the heat shock protein 70 family. Ssb-type Hsp70 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.10" evidence="2"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041164.1">
        <property type="nucleotide sequence ID" value="XM_019186088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAQ6"/>
    <dbReference type="STRING" id="683960.A0A1E3PAQ6"/>
    <dbReference type="GeneID" id="30203334"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd24093">
        <property type="entry name" value="ASKHA_NBD_HSP70_Ssb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000014">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000004">
        <property type="entry name" value="Heat shock protein SSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000005">
        <property type="entry name" value="Heat shock protein ssb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ61957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="613" mass="66044" checksum="5FFF50400EF906FE" modified="2017-01-18" version="1">MAEGVFPGAIGIDLGTTYSCVATYDSAVEIIANEQGNRVTPSFVAFTSEERLIGDAAKNQAALNPKNTVFDAKRLIGRRFDDESVQKDIKTWPFKVIDSNGAPLVEVEYLDETKTFSPQEISSMVLTKMKEIAEAKIGQKVEKAVITVPAYFNDAQRQATKDAGAISGLNVLRIINEPTAAAIAYGLGAGKSEKERHVLIFDLGGGTFDVSLLHIAGGVFTVKATAGDTHLGGQDFDTNLLEHFKAEFKKKTGNDISGDSRALRRLRTAAERAKRTLSSVATTTVEVDSLFDGEDFASNISRARFEDINAALFKSTLDPVEQVLKDAKISKDKVDEVVLVGGSTRIPKVQKLLSDFFDGKQLEKSINPDEAVAYGAAVQGAILTGQSTSDDTKDLLLLDVSPLSLGVAMQGNVFAPVVPRNTTVPTIKRRTFTTVADHQTTVQFPVYQGERVNCAENTLLGEFDLKGIPPLPAGEPVLEAIFEVDANGILKVTAVEKSTGKSANITISNAVGRLSSADIEKAIEEAEKFKSSDEAFSKRHEAKQRLESYVSSIEATVTDPVLSSKLKRGSKDKIEAALSDALAALEIEESTADDYRKAEVGLKRVVTKAMATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAZ0</accession>
    <name>A0A1E3PAZ0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_26453</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat G protein beta family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041792.1">
        <property type="nucleotide sequence ID" value="XM_019181263.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAZ0"/>
    <dbReference type="STRING" id="683960.A0A1E3PAZ0"/>
    <dbReference type="GeneID" id="30198509"/>
    <dbReference type="OrthoDB" id="10255630at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120171">
        <property type="term" value="C:Cdc24p-Far1p-Gbetagamma complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031680">
        <property type="term" value="C:G-protein beta/gamma-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005937">
        <property type="term" value="C:mating projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001965">
        <property type="term" value="F:G-protein alpha-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031682">
        <property type="term" value="F:G-protein gamma-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097110">
        <property type="term" value="F:scaffold protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043577">
        <property type="term" value="P:chemotropism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061951">
        <property type="term" value="P:establishment of protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903260">
        <property type="term" value="P:protein localization to mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016346">
        <property type="entry name" value="G-protein_beta_1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19850">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN BETA G PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25391">
        <property type="entry name" value="WD40_Gbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002394">
        <property type="entry name" value="GN-bd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="65"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="158"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="199"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="242"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="315"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="351" mass="38901" checksum="CB6000283A8E746E" modified="2017-01-18" version="1">MSPQQDDPQEYITKQLNKARLESRQLYAQIDKVKAKIQDTTLLDSATQIPSLSKDSINLKPLQNLKGHNNKIADMKWSKDSKSILSASQDGFLIIWDPTTGLKLNAVPLESQWVLSCAYSPSAQLVASAGLTNNCTIYRISSNLQDEFAFQQRIVSIFKGHTCGITACDFINDEEILTASGDMTCALWDIKKGKKTAEYLDHLGDVLCMDINKSDTNQLVSGASDGYARIWDRRSSNLEQSFFVSNSDVTSVKFFKDGNSIVTGSDDGVIRLFDLRADCITSLDFSQSGRLMYSCYTEHGCMVWDLLKAEIVGKLEGHNNRVSHVATSPNGLAVATASWDTTMRVFTPSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB40</accession>
    <name>A0A1E3PB40_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_76806</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041843.1">
        <property type="nucleotide sequence ID" value="XM_019184452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB40"/>
    <dbReference type="STRING" id="683960.A0A1E3PB40"/>
    <dbReference type="GeneID" id="30201698"/>
    <dbReference type="OrthoDB" id="551302at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007062">
        <property type="entry name" value="PPI-2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04979">
        <property type="entry name" value="IPP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="227" mass="26820" checksum="17B6A027A3409DE1" modified="2017-01-18" version="1">MAGGILKNKLEQDQQDNLKLTEDTKEFRNQVLKNTKLNALLKNNIDPTILNDQGDIDPSKISQLSDDDKEKLKWNAKNLQDNEIIQKQIIENLGQTIDEPKTPFQKAAQPELNEYYQDDDDVEDLNSFSLGEPEIKVDELDQKFDVKVHPEEEEEEQEEEKPKKSFAELRKQHYHNEVPHHKDVEFEEEDDDDKEEETPQQKKTFEELRKQHYHHEHVPVLEKPDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB51</accession>
    <name>A0A1E3PB51_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gag1-like clamp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_60699</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041850.1">
        <property type="nucleotide sequence ID" value="XM_019183037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB51"/>
    <dbReference type="STRING" id="683960.A0A1E3PB51"/>
    <dbReference type="GeneID" id="30200283"/>
    <dbReference type="OrthoDB" id="5576875at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053274">
        <property type="entry name" value="Fluconazole_resistance"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025124">
        <property type="entry name" value="Gag1-like_clamp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28065:SF1">
        <property type="entry name" value="DUF4050 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28065">
        <property type="entry name" value="FREQUENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13259">
        <property type="entry name" value="clamp_Gag1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gag1-like clamp" evidence="2">
        <location>
            <begin position="193"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="291" mass="31941" checksum="62264B13CD339E10" modified="2017-01-18" version="1">MAEGLNNSLNNKNIASKVNSNKDEASTTELKKQHTNKSFRSSRSIKSNSNNQIPTNQQQQQTASSSKKTVLKSIRNLFVKASINLSIAAAKLKSFAEDALSSDEIVNELFLVTSSSSVDSSLLEQQEAAATVANNQTATGEGARTGEQAETDSRAGTSDMTVNSSNEVGANGEDGLNGVFSLPRFQDFDAVQEAQKLQKENEVFCEGHQVWQKRRDVWLNSTSEERNKAKQKSLENSLIGVSKDNYPIIYNSLIEKQKTLKKPMNLKDALKIINAGWIASKKWERAAQGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB67</accession>
    <name>A0A1E3PB67_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">40S ribosomal protein S16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_66725</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041671.1">
        <property type="nucleotide sequence ID" value="XM_019184052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB67"/>
    <dbReference type="STRING" id="683960.A0A1E3PB67"/>
    <dbReference type="GeneID" id="30201298"/>
    <dbReference type="OrthoDB" id="426865at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000007">
        <property type="entry name" value="40S ribosomal protein S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001749">
        <property type="entry name" value="PRK00474.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF16">
        <property type="entry name" value="RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="16348" checksum="6E95BFE4BBFC04A9" modified="2017-01-18" version="1">MSEEKRVKSVQTFGKKKTATAVAHVKEGKGLIKVNGAPITLVEPEILRFKVYEPLILVGLDKFSNVDIRVRVTGGGHVSQVYAIRQAIAKGLVAYHQKFVDEQSKNELKKAFASYDRTLLIADSRRSEPKKFGGRGARARFQKSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBE5</accession>
    <name>A0A1E3PBE5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_82668</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041908.1">
        <property type="nucleotide sequence ID" value="XM_019185420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBE5"/>
    <dbReference type="STRING" id="683960.A0A1E3PBE5"/>
    <dbReference type="GeneID" id="30202666"/>
    <dbReference type="OrthoDB" id="4356994at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="27"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="762" mass="86626" checksum="C668DCA1B759F484" modified="2017-01-18" version="1">MELWTVDSYKKNSGVPKMRKRKRLTLTCTSCRQRKIKCDQGRPCSSCIKRKIPADMCIYNDTPFVNENGSDSPGGVAADDSNENNVKALKDEVEYLKKQLAELQHNGANSNTSTSGSTPFNSTNNPKLKLYGYLHSKPNKTVHFGPTSWRSLVDREENFDGLINEAKDYIKNVRKKWKFENDINKMSSDDYKSINVESPIMLLDNLARYLPCYEVVSNHVSRYVKGYWNRRTPIIDNDEFLKDFDGLFSQMSDGSCHIRIKNKSIDYAKIALVLIVVKHSMNTENAYSAIRQAYDSTDRMLLYANKLLELSNFMSKPSIPALQTLILLRQFKMINPSDDDGGDGHKGTLQFKTAINMAIVLGLHGDIDSIYRGYALNLRTLLKVIWKHLLFQDAILSFHMGIPLSINKSFINPSGLDDSDAYGKVTSCLRDAVDSLTSNECRQNDLLRIINKMESCHEGDLLHLSTTLRGLSEDTVLSDETCLVKIELKMFAIYTIHVLYDVLYQGTDSSNPDRELFYNGTMKYGVLTISHFIEAMIRFNKICMENSNTPEIFVLVEMCQSLVGVSKIIFLKVYTCLYTFELVRLVNINTDAQPTLQQVFSLSITEIERLKVDDRSSMLNISFKSPAFMHALMTLSCKYLLKLRNNSFGTVFSMNFCLLTVLAVFKYFEKFIDQKFHKQKHAINQLTKEKPIISATTFTATDDSFNDMVQKILYSGELDFFDVQYGENLSYDGLDDVLAGTGMFYDESTGNFTSSTSHNGPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBK4</accession>
    <name>A0A1E3PBK4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_76447</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041474.1">
        <property type="nucleotide sequence ID" value="XM_019184385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBK4"/>
    <dbReference type="STRING" id="683960.A0A1E3PBK4"/>
    <dbReference type="GeneID" id="30201631"/>
    <dbReference type="OrthoDB" id="2093493at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22849">
        <property type="entry name" value="NuzM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016813">
        <property type="entry name" value="NADH_Ub_cplx-1_21kDa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37325">
        <property type="entry name" value="OXIDOREDUCTASE 21 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G05910)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37325:SF1">
        <property type="entry name" value="OXIDOREDUCTASE 21 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G05910)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF022976">
        <property type="entry name" value="NADH_Oxi_21kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="187" mass="20878" checksum="560BAE87A2353ED8" modified="2017-01-18" version="1">MSGGPVPVYKKYTQGSKGIWEKIRQVLTLVPNRSSGNPVVPLYRVPAPGSQPKGKYYEDPYSLPAGDIQGNTYFKRDARRNYPKISAFDQTRVSGLLKLGTSTDPRISIGNTGEKELQVFKEPEAKIYLSSSLKAADDQIIQKQILGQYGVVVAPSLNLSKKSQVRIVKGDHGMYPENYPCRIFNYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC80</accession>
    <name>A0A1E3PC80_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translocation protein SEC62</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_26401</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC62 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042212.1">
        <property type="nucleotide sequence ID" value="XM_019181259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC80"/>
    <dbReference type="STRING" id="683960.A0A1E3PC80"/>
    <dbReference type="GeneID" id="30198505"/>
    <dbReference type="OrthoDB" id="200187at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031207">
        <property type="term" value="C:Sec62/Sec63 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071256">
        <property type="term" value="C:translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004728">
        <property type="entry name" value="Sec62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011553">
        <property type="entry name" value="Sec62_asco"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00869">
        <property type="entry name" value="sec62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12443">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12443:SF9">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03839">
        <property type="entry name" value="Sec62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="156"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="218"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="219"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="243"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ63005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="270" mass="30926" checksum="9B6CCDFFFD9F67CE" modified="2017-01-18" version="1">MAVNVAKFLRKNSLLKQRKGLLQGNLVDFFRFKRASRALLSEDYKKKQENPKNNLPPVRNETDAKGVFAMLIRNQLVHPGEKLSSAKAKENQLVPKKGTPWFLPLQKAELTPDEYYIWFYNEKSPWDIVLGFGVIAAVFTLILFPLWPAFMRKGVWYLSMAMLGLIALFFVIAIIRLIIFVITYVILSPGLWIFPNLFEDCGFIDSFKPFYAWNYPKEKKSKKSKKKAAAQSPLEQIPEKKTSTPASTSSSKPNQSTNVKRKATLEEVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC88</accession>
    <name>A0A1E3PC88_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_26085</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I NDUFA5 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042240.1">
        <property type="nucleotide sequence ID" value="XM_019181226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC88"/>
    <dbReference type="STRING" id="683960.A0A1E3PC88"/>
    <dbReference type="GeneID" id="30198472"/>
    <dbReference type="OrthoDB" id="286811at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006806">
        <property type="entry name" value="NDUFA5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12653:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12653">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 13 KD-B SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04716">
        <property type="entry name" value="ETC_C1_NDUFA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ63033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="15419" checksum="793997293CAB4232" modified="2017-01-18" version="1">MRFTPLLRSTQVLVRSATGNPTGLTGILTHPNPRPALIELYRSTLGFLDDKFPEESVYKKSTLNFTKKRLEIVEKHEEIDKIEAAIGNGLIEELLIQANDEYELAKKMVEWKAWEELEEKPLEDQWVYFGKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUF7</accession>
    <name>A0A1E3NUF7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]</fullName>
            <ecNumber evidence="10">1.3.1.88</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_36867</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>5,6-dihydrouridine(16) in tRNA + NAD(+) = uridine(16) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53380"/>
            <dbReference type="Rhea" id="RHEA-COMP:13543"/>
            <dbReference type="Rhea" id="RHEA-COMP:13544"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:53382"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>5,6-dihydrouridine(16) in tRNA + NADP(+) = uridine(16) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53376"/>
            <dbReference type="Rhea" id="RHEA-COMP:13543"/>
            <dbReference type="Rhea" id="RHEA-COMP:13544"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:53378"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5,6-dihydrouridine(17) in tRNA + NAD(+) = uridine(17) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53372"/>
            <dbReference type="Rhea" id="RHEA-COMP:13541"/>
            <dbReference type="Rhea" id="RHEA-COMP:13542"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:53374"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>5,6-dihydrouridine(17) in tRNA + NADP(+) = uridine(17) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53368"/>
            <dbReference type="Rhea" id="RHEA-COMP:13541"/>
            <dbReference type="Rhea" id="RHEA-COMP:13542"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:53370"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 5,6-dihydrouridine in mRNA + NAD(+) = a uridine in mRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69851"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:69853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 5,6-dihydrouridine in mRNA + NADP(+) = a uridine in mRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:69857"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Dus family. Dus1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.88" evidence="10"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035982.1">
        <property type="nucleotide sequence ID" value="XM_019182480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUF7"/>
    <dbReference type="STRING" id="683960.A0A1E3NUF7"/>
    <dbReference type="GeneID" id="30199726"/>
    <dbReference type="OrthoDB" id="272303at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102262">
        <property type="term" value="F:tRNA-dihydrouridine16 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102263">
        <property type="term" value="F:tRNA-dihydrouridine17 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF5">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(16_17) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="18">
        <location>
            <begin position="20"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="381"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="381"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="400"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ56775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="422" mass="48430" checksum="3B58A1B7DD1CD069" modified="2017-01-18" version="1">MTKLQGRELYNSIGNPKRIVAPMVDQSELAWRILSRRYGATLCYTPMFHAKLFATSEKYRKDMWSELDGDDSKDRPLVVQFCANDPEYLLQAAKFVVGKCDAVDLNLGCPQGIAKKGGYGAFLMDDWDLIYKLINKLHTELEIPVTAKIRVYEDKQKTLDYAKMVLSAGAQFLTVHGRLREQKGQKTGLADWEILQYLRESLPKDTVFFANGNILYPEDITRCLTKVNCDAVMSAEGNLYNPGVFMKESADLEKQFPRVDQLLREYYEIVKECPGNASKIAMKSHFFKILRPFLPHHTDIRQQIATCTPKHKFEEIEQIVINVEKIVQEIFAKPDIEELDKITTGEQQLWGGAYKTIPYWRCQPYFRPVDGVTGDKRVTDALKQKEEGKTAEVVESTSTEQDKNNKRRAEEELDNESKKQSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUM3</accession>
    <name>A0A1E3NUM3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial outer membrane protein OM14 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_81616</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035990.1">
        <property type="nucleotide sequence ID" value="XM_019185273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUM3"/>
    <dbReference type="GeneID" id="30202519"/>
    <dbReference type="OrthoDB" id="3980970at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039453">
        <property type="entry name" value="OM14_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17304">
        <property type="entry name" value="OM14_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Mitochondrial outer membrane protein OM14 C-terminal" evidence="4">
        <location>
            <begin position="123"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="56"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ56783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="191" mass="20528" checksum="CB009E76B05DF586" modified="2017-01-18" version="1">MSSYAEKAAESGPADNKKIPSPPEVKHSTPSGSVEAVEENAKKSALGGKAVVEEKVNSLKKDLPKLGEEAKKAEKEAAEKLKKKYDENKGPVLNFIRSISEKITKATDYVVAKTSSGAVVAKEELKNPAVATQTVVGIGAISAAIVALQDRQRIFRFKSDNEISLILAAVAGFVVLDGYLFSVFYPKYKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUR3</accession>
    <name>A0A1E3NUR3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">V-type proton ATPase subunit E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_86262</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036030.1">
        <property type="nucleotide sequence ID" value="XM_019186025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUR3"/>
    <dbReference type="STRING" id="683960.A0A1E3NUR3"/>
    <dbReference type="GeneID" id="30203271"/>
    <dbReference type="OrthoDB" id="10263003at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.30">
        <property type="entry name" value="ATP synthase, E subunit, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00311">
        <property type="entry name" value="ATP_synth_E_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038495">
        <property type="entry name" value="ATPase_E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45715">
        <property type="entry name" value="ATPASE H+-TRANSPORTING V1 SUBUNIT E1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01991">
        <property type="entry name" value="vATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160527">
        <property type="entry name" value="V-type ATPase subunit E-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ56823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="25857" checksum="719228DE9AB428F6" modified="2017-01-18" version="1">MSTARALSDTQVADELKKMESFIKKEADEKAKEIQLKADEEYEIEKANIVRSETSAIDALYEQKLKKASLTQQIAKSTIANKTRLKVLSTREKVLDDIFDEAEKQLKEISKDKSKYKPVLTGLIEEGLLSLLEKSVTVKVRKSDVEIAKEAIQEAAKNFEEKAKFPVEVNVDEENYLNDDIAGGVILVNGTGKIELDNTLEERLKLLSEESLPAIRLSVFGQSATRKFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV15</accession>
    <name>A0A1E3NV15_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_36397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036140.1">
        <property type="nucleotide sequence ID" value="XM_019182431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV15"/>
    <dbReference type="STRING" id="683960.A0A1E3NV15"/>
    <dbReference type="GeneID" id="30199677"/>
    <dbReference type="OrthoDB" id="10017101at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="38"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ56933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="264" mass="30331" checksum="397D8C2F96CF20B8" modified="2017-01-18" version="1">MSSTDQTYYRDGHDASISLTHSWRNVKNSCAYMLNFIKPTDKILDVGCGPGTITYDLGKYVPKGEIIGIEPTKELIDEALRDETTKKSQPNVSFEVCSVYSLPYPDSCFDIVHAHQVIVHLENPLKALQEMKRVLKPGGYICCRDGDLQACIFYPEVFKEPLFYYLEKATTEFTCWYGGSRLKELFIGVKFKPDKIIHTTSTWCISSRADRELFSNMYIKRLDSIKYNSSDKYTKSQLKEAWLQWANDDKASWVQIHGEVIAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV22</accession>
    <name>A0A1E3NV22_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">DNA replication complex GINS protein PSF3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_58643</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The GINS complex plays an essential role in the initiation of DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the GINS complex which is a heterotetramer of SLD5, PSF1, PSF2 and PSF3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GINS3/PSF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036256.1">
        <property type="nucleotide sequence ID" value="XM_019182828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV22"/>
    <dbReference type="STRING" id="683960.A0A1E3NV22"/>
    <dbReference type="GeneID" id="30200074"/>
    <dbReference type="OrthoDB" id="10251744at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11713">
        <property type="entry name" value="GINS_A_psf3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21693">
        <property type="entry name" value="GINS_B_Psf3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010492">
        <property type="entry name" value="GINS_Psf3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038437">
        <property type="entry name" value="GINS_Psf3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055221">
        <property type="entry name" value="PSF3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22768">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22768:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22466">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
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        <location>
            <begin position="4"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="GINS subunit" evidence="8">
        <location>
            <begin position="76"/>
            <end position="174"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006343"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011352"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00015140"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU367161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="177" mass="20320" checksum="FAEE14493C9F6348" modified="2017-01-18" version="1">MSYYDLDDIISHGQKIPCKFNIDVKGLGYLEGNPGKNIAKDTKLELPMWIAEVLAVCMTSEESSTAFIELIQPEAINTKVINAIKTSSTSLDVHSISQHYYALVQKWGELFTDKELVDVVQQMLKERAEEINNHAQSLRGIKQESSFLYTLDEFEKKLYKTSHESHKNLRKWLHNES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVG5</accession>
    <name>A0A1E3NVG5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7907 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_65363</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ57103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVG5"/>
    <dbReference type="STRING" id="683960.A0A1E3NVG5"/>
    <dbReference type="GeneID" id="30201145"/>
    <dbReference type="OrthoDB" id="4018368at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="DUF7907"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25484">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133537" description="DUF7907 domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7907" evidence="2">
        <location>
            <begin position="29"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="168" mass="18659" checksum="88AB4796B7F976D4" modified="2017-01-18" version="1">MKFSTILTTAATSLALAQARTVRFFTESNDTQINHKGLYSTHEGAGINYMFLGGDDAQNYTITDYGEIFLSQDSADGSTFKQTLSIVNYPNVFPYLQLSVTPDNVNWYIDDGYLSGNGSDNFWVAKNTSDPYNFSQYSYTIGLFLNGSTDPKQYDAAARVKILADIQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVH3</accession>
    <name>A0A1E3NVH3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Membrane anchor Opy2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_81318</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ57094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036301.1">
        <property type="nucleotide sequence ID" value="XM_019185214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVH3"/>
    <dbReference type="STRING" id="683960.A0A1E3NVH3"/>
    <dbReference type="GeneID" id="30202460"/>
    <dbReference type="OrthoDB" id="2402916at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018571">
        <property type="entry name" value="Membrane_anchor_Opy2_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09463">
        <property type="entry name" value="Opy2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane anchor Opy2 N-terminal" evidence="3">
        <location>
            <begin position="32"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="364" mass="39369" checksum="EAC635DF00ECE145" modified="2017-01-18" version="1">MGLTRSLNIPTEIIKRNEASSASPTATDANGCVVCSSDPVCPKCEDGEDCLLTIQTCQQCPKTYCKAVGGRSHSSGPSAQTVGGLAGGITGGLLLLIGIGSYIFYKYYVKKKLVFNRVNHKEFYFDDDVEVNFRHNHPGNDGQQPPNQGSSRNSLATTMFTRASNIIPIAYIPGVTIGPNTDSGNSRASQYSETNTIDSELIGDRFSKASIIGNPSLTTTAIRAKPKLVNIHDANHGNKNLPDANRDAVPSTAVSASQLGGVKSIKVSDKKKYNPGLQTEILEEESDNESVMPVDSKNVDYEPFIIEEEEEGEDEDTSAENMTTERKSTRRDSNDSAGSVILEVEVDRNPMSPFDDKFTLDDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVH6</accession>
    <name>A0A1E3NVH6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Halotolerance protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_17419</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036355.1">
        <property type="nucleotide sequence ID" value="XM_019180741.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVH6"/>
    <dbReference type="STRING" id="683960.A0A1E3NVH6"/>
    <dbReference type="GeneID" id="30197987"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045807">
        <property type="term" value="P:positive regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903329">
        <property type="term" value="P:regulation of iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13994">
        <property type="entry name" value="STKc_HAL4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000183">
        <property type="entry name" value="Serine/threonine-protein kinase hal4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF558">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="116"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ57148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="392" mass="44673" checksum="65727B5285A6481A" modified="2017-01-18" version="1" fragment="single">NANNSSSANLKNLPRFLVYEDGSHEHHLRNAKRQEKLGNMLKNLLGAQKLRDEAVSAVPEILSNNPPSLFSGLVNQFSNVKQHEENFPFSGATRVQGVQASANSTSHSKTFVERYGRCQEVIGKGTFGVVRISHKKFNNSEKETLFAVKEFKRRPTETEQKYSRRLTSEFCISSSLKHVNIIETLDLLQDAKGDYVEVMEFCSGGDLYTLIIAAGKLEYLEADCFFKQLIRGVVYMHEMGVAHRDLKPENLLLTEKGILKITDFGNGECFKMAWEKDIHFSGGICGSSPYIAPEEYTTKEFDPRSVDIWACGVIYMAMRTGRQLWRVAKDEDEFYLKYLEGRKSTKGYEPIEHLKRARCRNVIYSILDPVPSRRINGKQILNSEWGREIKCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWE9</accession>
    <name>A0A1E3NWE9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_35529</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036650.1">
        <property type="nucleotide sequence ID" value="XM_019182321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWE9"/>
    <dbReference type="STRING" id="683960.A0A1E3NWE9"/>
    <dbReference type="GeneID" id="30199567"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005619">
        <property type="term" value="C:ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990317">
        <property type="term" value="C:Gin4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001400">
        <property type="term" value="C:mating projection base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032160">
        <property type="term" value="C:septin filament array"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031107">
        <property type="term" value="P:septin ring disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000196">
        <property type="entry name" value="Cell division control 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="6">
        <location>
            <begin position="104"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="465"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="485" mass="54806" checksum="3448D00E742A5D9F" modified="2017-01-18" version="1">MVESTNTHNPAHADAVDEAPIKESESATNVQIPSEPHTTSAESNGNGSAGAGGLEPPTDEAPNLKSNGPTVTTTPEIKIIRRKLNGYVGFANLPKQWHRKSIRRGFNLNLLSVGESGLGKSTLINTLFNKELYQPKEPRDPAVERPTGVSIDTISSEIEENGVKLNLTVIDTPGFGDQVNNTDSWQPIVDEINSRFDSYLEFENKINRTTVADTRIHACLYFIEPTGHSLKPLDIEFLKQVHKKVNVIPVVAKSDTLTEEEILEFKQRVLDDIKTQGIEIFEPKIYENDDEETILATKEILSKVPFAIVGSTTEIQTADGRTVRGRQYPWGVIEVDNEDHNDFVRLRQLLIRNFLEELREKTANVLYEAYRSEKLEKLGIPQDHSVFREFDPSIKQQEEKALHEAKLAKLEAEMKQIFQQKVSEKEKKLQKSEAELFSRHKEVKEKLLKQIKSLEEKKKQLENARLNAYEPAPAPAPKAKKGFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWH4</accession>
    <name>A0A1E3NWH4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">T-complex protein 1 subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_35553</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036557.1">
        <property type="nucleotide sequence ID" value="XM_019182326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWH4"/>
    <dbReference type="STRING" id="683960.A0A1E3NWH4"/>
    <dbReference type="GeneID" id="30199572"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03337">
        <property type="entry name" value="TCP1_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000037">
        <property type="entry name" value="T-complex protein 1 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000073">
        <property type="entry name" value="T-complex protein 1 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000005">
        <property type="entry name" value="T-complex protein 1 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012719">
        <property type="entry name" value="Chap_CCT_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02344">
        <property type="entry name" value="chap_CCT_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ57350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="528" mass="58428" checksum="D12A335D54324F44" modified="2017-01-18" version="1">MQAPVVFMNTSSERQTGRQAQISNITAAKAVSDIIRTCLGPKAMLKMLLDPMGGIVLTNDGHAILREIDVAHPAAKSMIELSRTQDEEVGDGTTTVIILAGEILAQTFPYIEKNIHPVIIIQALKQALSDALEIIHQVSRPIDIENEPAMIKLISASIGTKYISKWSDLMCSLALKAVKTVLIDDGYGHKEIDTKRYVRIEKLPGGEIEDSEVLQGVLLNKDVTHPKMKRYIENPRIILLDCPLEYKKGESQTNIEITKEEDWSRILQIEEDQVKQLCETILNFKPDLVITEKGVSDLAQHYLLKGGVTALRRVKKSDNNRISRATGATIVNRVEDLRESDVGTNCGLFKVEQIGDEYFSFLTKCSNPLACTVMLRGPSKDILNEIERNLQDAMAVTRNVIFEPSLSPGGGATEMAVSVKLSEKAKTIEGVQQWPYQAVADAFEVIPRTLIQNAGGNPIRILSQLRAKHAKGDYTFGIDGDKGKVVDMNSYGIWEPEVIKQQSIKTAIESACLLLRVDDIVSGVRKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWX1</accession>
    <name>A0A1E3NWX1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">TFIID subunit TAF5 NTD2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_35585</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat TAF5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036792.1">
        <property type="nucleotide sequence ID" value="XM_019182331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWX1"/>
    <dbReference type="STRING" id="683960.A0A1E3NWX1"/>
    <dbReference type="GeneID" id="30199577"/>
    <dbReference type="OrthoDB" id="10266330at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08044">
        <property type="entry name" value="TAF5_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.500">
        <property type="entry name" value="TFIID subunit TAF5, NTD2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007582">
        <property type="entry name" value="TFIID_NTD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037264">
        <property type="entry name" value="TFIID_NTD2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF1">
        <property type="entry name" value="CANNONBALL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="TFIID subunit TAF5 NTD2" evidence="10">
        <location>
            <begin position="131"/>
            <end position="261"/>
        </location>
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            <begin position="391"/>
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        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
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        </location>
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        </location>
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        </location>
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            <begin position="1"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00009435"/>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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        </source>
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        <source>
            <dbReference type="Pfam" id="PF04494"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ57585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="726" mass="81806" checksum="972DC9BEBFC17F22" modified="2017-01-18" version="1">MSQNPQVKQEGGSVNSPGQIPNNNIRPGAQQPVQGQASNQPGTPQQQQQAQQQAQQQAQAQQQRRANPQFSPADLNRIVLEYLNKKGYHKTEAVLRVESSHTPTPPIAPQPSTNINRSIPDKQVQSNEKFDPEIYERGYSMLRTWVESSLDIYRPELYRVLYPIFVHCFLDLIYKNAKVEARRFFDKFAIDHEILHGTEINKLAGIGSIDHLEENELAKTFRKNTYVLHVTRTSLNLLLYFLHENEALGGSIMISLINRYIQPKIITGRVDTQIGEGSLDDGIKSAEEIEKFNSQPVKLGNLPMDPEYSKEVEVELKRRDDKEGVTEDTLVEEFSKLKKETEDSPARETLPLPQRTALDLKREIIAVRESRDKIKLDAIQASAPSVCMYTFHNTNNDLTTLEFNDDSTLIAGGFQDSYIKLWSLDGSPLTSILKNDEHSDENTRKLIGHSGSIYGLSFSPDNRYLLSSSEDKTTRLWSLDTYTPLVSYKGHNHPIWDVKFSPFGHYFATASHDQTARLWSCDHIYPLRIFAGHLNDVDTVEFHPNSTYVFTGSSDKTCRMWDISKGNTVRIFNGHTGPVNTMAVSPDGRWLASAGEDSIINIWDIGSGRRLKSMKGHGRNSIYSLAFSKEGSILISGGADNSVRVWDIKRGTNEAGPIPEPFPSQTDDPTTSINVNSKEKMEEIRRRKEVVPSADHLASFFTKRTPVYKVHFTRGNLVLAAGAFNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX02</accession>
    <name>A0A1E3NX02_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Maintenance of telomere capping protein 4</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">WICANDRAFT_97140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038769">
        <property type="entry name" value="MTC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38426">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38426:SF1">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="631"/>
            <end position="649"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        </location>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX42</accession>
    <name>A0A1E3NX42_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_35492</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019182312.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="30199558"/>
    <dbReference type="OrthoDB" id="407617at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000024">
        <property type="entry name" value="Putative membrane protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF37">
        <property type="entry name" value="PQ-LOOP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="239"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="288" mass="32794" checksum="61FE9E3F37F624C2" modified="2017-01-18" version="1">MGNEKAATVLGTLGTVCWCIQLIPQVYYNYRRKNCEGFPPLMMFLWAACGIPFSIYFMSTRSNVSLQVQPEVFFCFCTIAWIQTLYYPPVQLSKKKITLMVASFILFAIGCQVGFILWLRPLHDRGITWPTLIFGVIASVLLAVGLLPPYFELAKRKGRVVGINFWFIGIDCAGAFFSMLSVVVGSMDIMGIILYCVCMALEVGIFTSHFIWCLRFKWFRKSDDLESNDDVELGLGDKESLEKKRSHEVSSEILDHRADTHSAVDNEETRTVESTEEHKEHEITTKEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXK8</accession>
    <name>A0A1E3NXK8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">4-hydroxybenzoate polyprenyltransferase, mitochondrial</fullName>
            <shortName evidence="10">4-HB polyprenyltransferase</shortName>
            <ecNumber evidence="10">2.5.1.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">4-hydroxybenzoate hexaprenyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Para-hydroxybenzoate--polyprenyltransferase</fullName>
            <shortName evidence="10">PHB:PPT</shortName>
            <shortName evidence="10">PHB:polyprenyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">COQ2</name>
        <name evidence="11" type="ORF">WICANDRAFT_101315</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of coenzyme Q (CoQ) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>an all-trans-polyprenyl diphosphate + 4-hydroxybenzoate = a 4-hydroxy-3-(all-trans-polyprenyl)benzoate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:44504"/>
            <dbReference type="Rhea" id="RHEA-COMP:9514"/>
            <dbReference type="Rhea" id="RHEA-COMP:9564"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
            <dbReference type="ChEBI" id="CHEBI:78396"/>
            <dbReference type="EC" id="2.5.1.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Mitochondrion inner membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
            <orientation evidence="2 10">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.39" evidence="10"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037136.1">
        <property type="nucleotide sequence ID" value="XM_019180317.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXK8"/>
    <dbReference type="STRING" id="683960.A0A1E3NXK8"/>
    <dbReference type="GeneID" id="30197563"/>
    <dbReference type="OrthoDB" id="18170at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008412">
        <property type="term" value="F:4-hydroxybenzoate polyprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13959">
        <property type="entry name" value="PT_UbiA_COQ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1780:FF:000001">
        <property type="entry name" value="4-hydroxybenzoate octaprenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.140:FF:000003">
        <property type="entry name" value="4-hydroxybenzoate polyprenyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01635">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006370">
        <property type="entry name" value="HB_polyprenyltransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039653">
        <property type="entry name" value="Prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01474">
        <property type="entry name" value="ubiA_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048:SF28">
        <property type="entry name" value="4-HYDROXYBENZOATE POLYPRENYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048">
        <property type="entry name" value="PRENYLTRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="263"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ57929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="335" mass="37046" checksum="917BAF9D177E25CD" modified="2017-01-18" version="1">MLRSHSIFLLQSSIRFTQKERDEAAALRLKSLGPYVSKLPTSWIPYAELARLDKPVGTWLLYIPCTWSILIGAQQIGAPLGSTLGMLAIFGTGALIMRGFGCTVNDLLDKNLDNKVLRTIERPIASGRVSPFQAKMFAGGQLAAGLAVILQIPWDCFLLGSSSLFLVCTYPLFKRFTYYPQAVLSLAFNWGALLGFPAMGVWDPNVMIPLFLSSFCWTMTYDTIYAHQDKKFDINAGIKSTALKWKDHSKKIFVGLTATQLSLLALTGVNAGIITGPGFIIGSGIFAYRLVQMIRKVDLNDPKSCWSHFTGNISSGLYFSYGLFFDYLLQVFGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXW7</accession>
    <name>A0A1E3NXW7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_69992</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037215.1">
        <property type="nucleotide sequence ID" value="XM_019184189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXW7"/>
    <dbReference type="GeneID" id="30201435"/>
    <dbReference type="OrthoDB" id="10614765at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="470"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="471"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="496" mass="56614" checksum="7E0D68C5B0D7B56A" modified="2017-01-18" version="1">MNHVALQNSESTLTRPQTSRTHSLETRGRSTSIRSNNQEQGIMQHQQSEQIYQFRPPEVVKNIQKVPNVRDPTFEKSRSRETSTVRKDYLSNVEIIDMRRSSSAASTNFNRPSRVPSNLNPNPQYPSTHHVNNRYRNPMSAYSSNEAVDTIVPNHSNILYQKYQRPSITSKPSIESKAPSTISQYQTPRSNVSVSMDQDLVPAFNDLKMELGDAMNDHIDKQTDTFQTDNRYVNSDSNSTKSNNSENPSLLNDANTSPEEPIFKTSISEEISDEKQLPKHVEKFGRIQQRVNFLKQMKDEDSNNSAWKSVSVEQKMFFESLNNDLRNLKRSNHKPFLKSLERTDQSEKQVLNSSASVTSTQYLLKTYNPMTEGKHHSLAKPHPTPTKEIDIGKLKQLLSQSTKLAEMMWEAEEEKYRPSQITFKSENKSSTDTAVSSLNSEAFSVKLVSHTYEPKDLDAIKESFDGYEISTTHHNETGDVRRMNDSKQASPLSQRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY45</accession>
    <name>A0A1E3NY45_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mso1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_80239</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037285.1">
        <property type="nucleotide sequence ID" value="XM_019185026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY45"/>
    <dbReference type="STRING" id="683960.A0A1E3NY45"/>
    <dbReference type="GeneID" id="30202272"/>
    <dbReference type="OrthoDB" id="3981242at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033101">
        <property type="term" value="C:cellular bud membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032120">
        <property type="term" value="P:ascospore-type prospore membrane formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061025">
        <property type="term" value="P:membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028095">
        <property type="entry name" value="Mso1_N_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14475">
        <property type="entry name" value="Mso1_Sec1_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mso1 N-terminal" evidence="2">
        <location>
            <begin position="24"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="186" mass="21008" checksum="76EE8BF645A0D626" modified="2017-01-18" version="1">MSLHHDKGNFWTKVRSSTNKISSSLANLSIRSEHDGDSPNSTLIHKALVNHYVSKNEQFPQWLGEEQENHQHHNHPQRAQPQNTAQEQIRQPVQTQRFQPQSQQQQQQQQQGTPGTSRFASRFANDQNTRIPSRFATSGADHGSNGGSRFAGSNTRPGHPERSDSSSGSAMKDRLKRNNYRNNFGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY68</accession>
    <name>A0A1E3NY68_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_70083</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037323.1">
        <property type="nucleotide sequence ID" value="XM_019184192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY68"/>
    <dbReference type="GeneID" id="30201438"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="316" mass="36726" checksum="682C3A61D7ADDE4B" modified="2017-01-18" version="1">MTTLVKNNADTEPESESERSKESEDENTNAENINRNKILMTSLKHSQETEDKREMSDSNGSNNQQRLWKKPTNRVNKGQGYKSKSTTSNSLLETNFTTLDEAEYIRYYRQNHKNSNAKILSNILKEMNQEIGELKPDSDYLKWFHLKLNQPSQSSTHNMCAFKHHNSKINNGMKFDFSMLEKLTTINLLVLDLKDINHYKKMCKTNFKYNKPLLPILSTKDAIIYDSKIRNDLNQDVSTSSINGELQDPENKRRLTSDEDMKNKEEVDKQIKSKSTENEEGKINVGRVTRPKLKLSETAKEDEMRETTETDDKEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYK2</accession>
    <name>A0A1E3NYK2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 15">Ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="8 15">5.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_35321</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="17">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate degradation; pentose phosphate pathway; D-xylulose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 15">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="8 15"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036864.1">
        <property type="nucleotide sequence ID" value="XM_019182294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYK2"/>
    <dbReference type="STRING" id="683960.A0A1E3NYK2"/>
    <dbReference type="GeneID" id="30199540"/>
    <dbReference type="OrthoDB" id="1927044at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00411"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000074">
        <property type="entry name" value="Ribulose-phosphate 3-epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02227">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026019">
        <property type="entry name" value="Ribul_P_3_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004076">
        <property type="entry name" value="PRK05581.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01163">
        <property type="entry name" value="rpe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="RIBULOSE-5-PHOSPHATE-3-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001461">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01085">
        <property type="entry name" value="RIBUL_P_3_EPIMER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="14 17" id="KW-0170">Cobalt</keyword>
    <keyword evidence="12 15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="10 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 17" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="16">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="152"/>
            <end position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="203"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00009541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00013920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR001461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ57657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="234" mass="25290" checksum="2ED7F979E268C854" modified="2017-01-18" version="1">MVKSIIAPSILASDFSNLGCECHKVLGAKADWLHIDVMDGHFVPNITLGPPIVDSLRKTIPRGDGVTKPKAFFDCHMMVAEPEKWVEEFVKVGADQFTFHYEATKDPLGLVKLIKSHGIRAACAIKPGTPVDVLFELAPYLDMALVMTVEPGFGGQKFMADMMPKVQTLREKFPNLDIQVDGGLGKATIPAAAEAGANVIVAGTSVFTAEDPAETIKFLRDAVDVQLKARGLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYU6</accession>
    <name>A0A1E3NYU6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_33428</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERT1/acuK family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037573.1">
        <property type="nucleotide sequence ID" value="XM_019182068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYU6"/>
    <dbReference type="STRING" id="683960.A0A1E3NYU6"/>
    <dbReference type="GeneID" id="30199314"/>
    <dbReference type="OrthoDB" id="2538135at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045991">
        <property type="term" value="P:carbon catabolite activation of transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045013">
        <property type="term" value="P:carbon catabolite repression of transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045722">
        <property type="term" value="P:positive regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050335">
        <property type="entry name" value="ERT1_acuK_gluconeogen_tf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056751">
        <property type="entry name" value="PAS_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47659:SF1">
        <property type="entry name" value="TRANSCRIPTION ACTIVATOR OF GLUCONEOGENESIS ERT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47659">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24990">
        <property type="entry name" value="PAS_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="12">
        <location>
            <begin position="15"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="13">
        <location>
            <begin position="225"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="112"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ58366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="343" mass="39427" checksum="1002CF96BC407699" modified="2017-01-18" version="1" fragment="single">PAQRNKPVRKKTARACQHCHKAHMTCDESRPCKRCVARGWQETCVDAPRKKKKYLMDVPDELEQQTTPNSYSMNISPSIESPAQNTASQLNDFAADLEYSILSDIIKQDYLSPSGMRHSPSQSFTDQTQSPPGPYPQQQQQQQHNTYLDGFRNNSTHNIYAKINKPFSYTPGFHALINYLRNRFNKKELVKMAKSMANYRPSFIACTNTLKEEDLIFMEQCFQRTLLEYDKFISISGTPTIVWRRTGQIAYVSEEFCILTGWTKEQLLNKITFIVELMDDVSVVEYFDLFSTIAYGDFRGATMTGCTLLTPENTTMKTTCMWTLKRDVFGIPMMIIGNFLPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYV5</accession>
    <name>A0A1E3NYV5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_81095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036974.1">
        <property type="nucleotide sequence ID" value="XM_019185175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYV5"/>
    <dbReference type="GeneID" id="30202421"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="300" mass="35081" checksum="D5074E07520B54C7" modified="2017-01-18" version="1">MRQYSSYWSIEKFAKSGEGYKTVRQLKERRPARNIDPVLITTMNTPAPVQLVPQEVVQAYLDLTAHNSPFKNIPRYVPTDIKYSGYGYYNPPISEIHQVVLFHLKAKVNGTADHTFNFLAAQQAKESKETKETDPFTLKSLSVCLPIVLDKKYIVIRRQAKAEAADIFTPQETKQAFIHQDNFFQQTRFKKNNLEFKLSKYTSYDPESVLILRIRYQVLEHLDESVMHIFEHIKKPHPMKVIRLFDYPETIFDHNKEESNPTRQFFVIAKRPADQKEVPIPVEFKEPNVRCMVIDTSGWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ86</accession>
    <name>A0A1E3NZ86_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Branched-chain-amino-acid aminotransferase</fullName>
            <ecNumber evidence="16">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_80660</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 2-oxocarboxylate + L-methionine = 4-methylsulfanyl-2-oxobutanoate + an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:31763"/>
            <dbReference type="ChEBI" id="CHEBI:16723"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:31765"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 6/6.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 14">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="16"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037733.1">
        <property type="nucleotide sequence ID" value="XM_019185105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ86"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ86"/>
    <dbReference type="GeneID" id="30202351"/>
    <dbReference type="OrthoDB" id="1732691at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052656">
        <property type="term" value="F:L-isoleucine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052654">
        <property type="term" value="F:L-leucine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052655">
        <property type="term" value="F:L-valine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010326">
        <property type="term" value="F:methionine-oxo-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000004">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000005">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009897">
        <property type="entry name" value="PRK13357.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825:SF44">
        <property type="entry name" value="BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825">
        <property type="entry name" value="SUBGROUP IIII AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 16" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="10 16" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9 15" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="13">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR006468-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ58526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="388" mass="43069" checksum="25DED78085C67160" modified="2017-01-18" version="1">MSSSALHTSSKAANLAKLDASKLKVTKNPNPKTPLPNDKLVFGQTFTDHMLTVKWNNEKGWDAPEIVPYGPFQMDPSSCVFHYAFELFEGLKAYRDSNDQIRMFRPDKNMVRMNKSADRIVLPTFDSEELIKAIGELIKIDKHLVPKDKGYSLYIRPTLIGTTAGLGVGIPSDALLFVITSPVGPYYSTGFKAVKLEATDYAVRAWPGGVGDKKLGANYAPCIKPQLEAASRGYQQNLWLFGEEKYVTEVGTMNVFFVFKDSTTGKKELVTAPLDGTILEGVTRDSILELARERLDPNEWAVSERYYSIYEIRDRAAKGELVEAFGSGTAAVVSPIKAIGFEGEDIQVPLLPGEQFGQLTNDVAQWIAKIQYGDEEYKNWSRVVADLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZH0</accession>
    <name>A0A1E3NZH0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_79110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037770.1">
        <property type="nucleotide sequence ID" value="XM_019184842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZH0"/>
    <dbReference type="STRING" id="683960.A0A1E3NZH0"/>
    <dbReference type="GeneID" id="30202088"/>
    <dbReference type="OrthoDB" id="1929311at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032704">
        <property type="entry name" value="Cms1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14617">
        <property type="entry name" value="CMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="236" mass="27181" checksum="973D8590AAA578B5" modified="2017-01-18" version="1">MSNNPDDLDDGLDYQVDSEPEAEGVEIKEDEQSEDEVVKNDQDNNEGKPSAKRRRKTDDKLKEKKRVKMEQDMKLKKKIPQLNSTELGHHFSQLLIKYNDDATHLDYFTKSDFVDASKYKENRNLDNFKDFTDKYNKGLTLIVSISRVRIGDLFKALGPKSKAIKVGKGYDGKIRDDTKYVIGTVERLLNLSFEGHEIKSIILDASYQDQKLHSILDEAKLTTLLKKYKGTKIILY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZL0</accession>
    <name>A0A1E3NZL0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_34168</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037661.1">
        <property type="nucleotide sequence ID" value="XM_019182157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZL0"/>
    <dbReference type="GeneID" id="30199403"/>
    <dbReference type="OrthoDB" id="411123at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018647">
        <property type="entry name" value="SLFN_3-like_DNA/RNA_helicase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09848">
        <property type="entry name" value="SLFN-g3_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="70"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="458" mass="52909" checksum="14827CBA47360913" modified="2017-01-18" version="1">MTTAKAFTSRKRPSGNTNKRNTKRSDNNQPFIPSAAVKKISQIQLSDEQQVLKSNIFKFINDNLENYTGNPSVYLIDGDAGTGKSVILNSLFNEIQKISRTSSDAEKQILTNTKNYLVVNHPEMLKLYHQISRQFPYILKTDLERPTSLINKFQKNKDTDKVDVLIIDEAHLLATAKDPFKRFNQDNHLEELLKISKIVIIVFDEKQSLRMGSYWSLNDSKDGASILPFLETQSLQSFESFNLTKQFRVSAKPDLIKWITELSTTKALLPLPIDDYESKTFDFKIFENCQEMYNSIKLQNSKFGQSRILATYDFPYRLDGKDYFVYSESDDFKLRWDRYLPQLTTPWSERDDTIDEVGSVYTIQGFDLNYAGVILGRSIGYDSTKDCIKIKPEFYDDNAGFTKKKNIHNVDKVKEKIILNSVNVLLTRGVKGLYIFAWDKELRERLMKGDSRVLVRNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZS0</accession>
    <name>A0A1E3NZS0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Carboxypeptidase</fullName>
            <ecNumber evidence="12">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_84369</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Release of a C-terminal amino acid with broad specificity.</text>
            <dbReference type="EC" id="3.4.16.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="12"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037785.1">
        <property type="nucleotide sequence ID" value="XM_019185701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZS0"/>
    <dbReference type="STRING" id="683960.A0A1E3NZS0"/>
    <dbReference type="GeneID" id="30202947"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046938">
        <property type="term" value="P:phytochelatin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.410:FF:000001">
        <property type="entry name" value="Carboxypeptidase Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008442">
        <property type="entry name" value="Propeptide_carboxypepY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF113">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE CTSA-4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05388">
        <property type="entry name" value="Carbpep_Y_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 12" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Propeptide carboxypeptidase Y" evidence="13">
        <location>
            <begin position="4"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ58578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="487" mass="55157" checksum="FD05763BF13100A7" modified="2017-01-18" version="1">MKLNSATDALNVPIDNLSQDVKTLWSEMTLKFPNAVSSLEFWSKPKPFTKKLNGWDFHVNNENLPNHSLRVKKTPESLGVDKVKQYSGYLDVEDEDKHFFYWFFESRNDPKNDPIILWLNGGPGCSSLTGLFFELGPSSIDEGIKPVYNKHSWNSNASVIFLDQPVNVGYSYSSSSITDTVAAGKDVYAFLELFFQQFPQYKDLKFHIAGESYAGHYIPVFASEILSHEERQFNLSSVLIGNGLTDPLTQYKYYEPMACGEGGEPSVLPEEECEAMLNSQDRCLSLIESCYESQSVWSCVPAAIYCNNAQMGPYQKTGRNVYDIREMCEGGSLCYTGLEYIDKYLNRDDVKEALGAEVDNYESCNFDVNRNFLFAGDWMKPYHKAVTDLLESKLPVLIYAGDKDFICNWLGNEAWTNVLPWSGADDFSSAKVKSWNVKGKEAGKVKNHENFTFLRVYGAGHMVPYDQPENSLDFFNNWIGGNYAINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZU7</accession>
    <name>A0A1E3NZU7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_84441</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037890.1">
        <property type="nucleotide sequence ID" value="XM_019185712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZU7"/>
    <dbReference type="SMR" id="A0A1E3NZU7"/>
    <dbReference type="STRING" id="683960.A0A1E3NZU7"/>
    <dbReference type="GeneID" id="30202958"/>
    <dbReference type="OrthoDB" id="5141738at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000044">
        <property type="entry name" value="Hexose transporter Hxt3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF75">
        <property type="entry name" value="GALACTOSE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="151"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="348"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="413"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="52"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ58683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="555" mass="62145" checksum="FB007DE344FF560C" modified="2017-01-18" version="1">MSTETSGQQTPVDEHHSDDHEKLENSSLESGHQQQGGIPERPLKEYFFISALCMMVAFGGFVFGWDTGTISGFVNQTDFKRRFGQINDHGEYYLSNVRVGLIVSIFNIGCAFGGIILAPLGDKFGRRIGLMCVMIVYIVGILIQICSFDKWYQYFIGRIISGLGVGAIAVLSPMLISETAPKALRGTLVSCYQLMITFGIFLGYCTNYGTKIYSDSTQWRVPLGLCFFWAILMIIGMVLMPESPRYLVEVGRLEDARHSIARSNQIHDDDPAVFGEVEIIQAGIEKEKSAGTASWGELVTGKPRILYRVVLGIMLQTLQQLTGDNYFFYYGTTIFKAVGLEDSFETSIVLGIVNFASTFVGLWVVERFGRRNSLLGGAAGMIACFVIFASVGVTRLYLNPEHTESSKGAGNCMIVFACIYIFFFASTWGPIIYTVVSETYPIRIRSKAMALATAANWLWGFLIGFFTPFITSAINFYYGYVFMGCLVFAFFYVFFFLPETKGLSLEEVHELYEEKVLPWKSTSWVPPSRRTAQQAAEQKEEIAAQEAPKFLKRFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZU8</accession>
    <name>A0A1E3NZU8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="15">ATP-PFK</shortName>
            <shortName evidence="15">Phosphofructokinase</shortName>
            <ecNumber evidence="15">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_43602</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15 16">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="15">Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 15 16">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterooctamer of 4 alpha and 4 beta chains.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="15"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037933.1">
        <property type="nucleotide sequence ID" value="XM_019182579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZU8"/>
    <dbReference type="STRING" id="683960.A0A1E3NZU8"/>
    <dbReference type="GeneID" id="30199825"/>
    <dbReference type="OrthoDB" id="537915at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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    <sequence length="964" mass="105240" checksum="E60CF3F6F68CCA05" modified="2017-01-18" version="1">MSVSGVAFTSFATKDLKTFEEAIGFYKSLGFKLIRNYTRDLNASSSLKGVSHDSLRETWLESFKISQVDPKNGARIPLQESNSIQSEGVVLKLRLTGSDLTPMESSSLLDGELNFFTTELEQVSKILGLKIESSKIITKDPLGNKISFVEHPTPFVPSKIDADFFASQLAQELSTESQDPESKKKKIAVMTSGGDSQGMNAAVRAVVRAGIFYGCDVFAVYEGYEGLVNGGEFIKQMAWEDVRGWLSLGGTLIGTARSKEFRERWGRLTAARNMISNGIDALIVCGGDGSLTGADLFRSEWPSLVDELVANGDFSEDEVAPHRHLTIVGLVGSIDNDMSGTDNTIGANSSLERICEMVDYIDATATSHSRAFVVEVMGRHCGWLALNAGLATGADYIFIPERAANKDTWKDELKQVCRRHREKGRRKTTVIVAEGALDSELNPITSEEVKDVLVELGLDTRITTLGHVQRGGSAVASDRMLATLQGVEAVKAVLDLTPEDPSPLIGVLENKIVRQPLVDAVKLTKSVAAAIEAKDFDKAISLRNTDFIESYQNFQSISQHDDGSHLLDASKQLNIAIIHVGASSAALNAATRAAALYALSRGHKPFAVQNGFSGLVRHGSMKPLTWIDVEDWHNLGGSEIGTNRTLPSQDIGTVAFYFQKYHIDGLIIIGGFEGFRALKELEEARNNFPVFNIPLTLIPSTVSNNVPGTEYSLGSDTCLNSLVSYCDAVKQSASSSRRRVFVVEVQGGHSGYVASYIGLVTGSVANYTPEKKITLKTIQEDIALLKENFLQDQGDIRSGKMLIRNETASSIFTTDLIADIIEESSNKRFETRTAIPGHVQQGGAPTALDRVNASRFAIKCAKFIEEWNEKISSNNLDGSDPMLRFKFVDGKKVSIVGDNEESACVIGIEGSELKFTSIKQLWENEADIKLRKGLTVHWEHFNEIADILSGRLILRSKSKSQGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZW8</accession>
    <name>A0A1E3NZW8_WICAA</name>
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        <recommendedName>
            <fullName evidence="14">Asparagine-rich protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_43408</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:cellular response to temperature stimulus"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="entry name" value="ERI-1_3'hExo_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RRM_ARP_like"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051274">
        <property type="entry name" value="3-5_Exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047201">
        <property type="entry name" value="ERI-1_3'hExo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034351">
        <property type="entry name" value="Nrp1_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23044">
        <property type="entry name" value="3'-5' EXONUCLEASE ERI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23044:SF61">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="254"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="11">
        <location>
            <begin position="353"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="11">
        <location>
            <begin position="433"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="395"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ58628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="462" mass="50995" checksum="FF26055343146462" modified="2017-01-18" version="1" fragment="single">MSDVFIVIHLATTCDENGVYVTKDSSEIIEIAWSLLDANTLEKLDSDSVLIRPINTPITALCTSITTLTWEHVRNAGTFRDAVLKLDQFIHEKITSKGLDFTFVTLNAWDLRVQLPREARDKSVVLPPYLQHSRCFDLKQEYSKWQAHHPETLSYSSSNLSSICTALEVEIEPQGNLAAVAAASSNGLNGNLPFHLQQLALQTPRRAIDETIVLEKILKSLIKKSQPIEDHSDVLSRPFDGRADVRAFLAERSKVLHLSNLPQDTTQSELESWFTQYGGRPIAFWTLKTPDQHKPTGLGFAVFSSHEEAAESLAMNGRALNDRSIEVQPSSARVLDRAQEILTPFPPSKNRPRPGDWTCPSCGFSNFQRRTACFRCSFPAASAAAIQESMYSSNGTNTPIGSSNGNSNNNNNNNGNGHGNGNGQSRVSSVPFRAGDWKCGNEGCSYHNFAKNICCLRCGAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P022</accession>
    <name>A0A1E3P022_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
            <ecNumber evidence="8">4.2.1.93</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">ATP-dependent NAD(P)HX dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_69165</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(6S)-NADHX + ATP = ADP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19017"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>(6S)-NADPHX + ATP = ADP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32231"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.93" evidence="8"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037960.1">
        <property type="nucleotide sequence ID" value="XM_019184158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P022"/>
    <dbReference type="STRING" id="683960.A0A1E3P022"/>
    <dbReference type="GeneID" id="30201404"/>
    <dbReference type="OrthoDB" id="8110916at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047453">
        <property type="term" value="F:ATP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000023">
        <property type="entry name" value="ATP-dependent (S)-NAD(P)H-hydrate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF C-terminal" evidence="9">
        <location>
            <begin position="12"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="181"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="220"/>
            <end position="224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="239"/>
            <end position="248"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="334" mass="36303" checksum="4914108E1C520B24" modified="2017-01-18" version="1">MSQLSQKSGKELLHLTKKLIPPLLPQFHKGQAGRIVVFGGCEDYTGAPYFAAHAAAVFGADLSHIICEKNAATVIKSYTPDLMVHPYLYEKDNVPNDVKDIDEFLGTKILTKVKALLARIHVAIVGPGLGRDELMLKTLEIVINYLREKKIPIIVDADGLFLVSQKPDVIKGYDLAILTPNVVEFKRLADAVGVDVNGDDQIEEAKQLSKKLGGVTILRKGSSDIIVKNDEALESTTKGSDRRAGGQGDSLTGTVATLLAWGHAYKTGLWEHPDEIQEDQLPLLACFGGSTVTRVAAREAFKAKGRAMQATDLHANVGKAYQIVFEDSNGEFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P045</accession>
    <name>A0A1E3P045_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_64447</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="415"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="582"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="415"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="484"/>
        </location>
    </feature>
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        <location>
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            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="583"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ58312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <sequence length="609" mass="68404" checksum="DD25BC16C2F99B76" modified="2017-01-18" version="1">MNTNDPLQDAQGSSSIVTAPTFQNAVNIPGANVSNPQLSLSKNELTNLPSLTSIPSSDLLVHPLNSSSSSASTFEKTLSATSSYADSVDSQRKSSNEDNNASAIVQLARRRRSSKSKEKHQRKNSALSRPQGRPLSKETRIRLTLVPTLGILYSILVSDTMRKTLKVLNYSDEENGMLLTNEELSTRNVISGFSSLLNVKTARFSEDGLSYSPEKNANIPKFASIDEAVYNLFGLNDYTLIRVSRAAATEVGGSVLLKIETAMPGYLYLNDDEDEDYKTVVENKAMYLEAIGSKGDRPSTTFLKKKVARPRYKSDMRIYLIPRINSCAIYCEKRMFKRDLLRGFLNMNKLDRSIIVAFNYQEAIEELEKEGYGDLIKEMKLPTVEEFNRSAHVPHSSNYPMSSVGLSISKQSEIQHLQHLQQQQQQQQQQQQQQFNHVPPPPHQQLYPGYPIQRPNEVPPFYDPAQPQPQLSYGFPQQSPSQIYQLPHHPQQPSSQANLFPQYNSYTYSQEVEPNQQPATQPGFSSMDQQFVQQPMEEMVGAHDPNRRINSQSVSYFNQQQMFPSQVMNPYSNIPHGQHFINPATAAPTATATAQSFPPAQDASGQYYQ</sequence>
</entry>
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        </recommendedName>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
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    </organism>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="27535936"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="entry name" value="Protein kinase activator Bem1"/>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
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    <dbReference type="InterPro" id="IPR035550">
        <property type="entry name" value="Bem1/Scd2_PX"/>
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    <dbReference type="InterPro" id="IPR035548">
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        <property type="entry name" value="Bem1/Scd2_SH3_2"/>
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        <property type="entry name" value="NADPH_Oxidase/PX-Domain"/>
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    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
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    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
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    <dbReference type="InterPro" id="IPR036871">
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        <property type="entry name" value="SH3-like_dom_sf"/>
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        <location>
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            <end position="65"/>
        </location>
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        <location>
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        <location>
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        <location>
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        </location>
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            <begin position="188"/>
            <end position="211"/>
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    <feature type="non-terminal residue" evidence="8">
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="50658" checksum="A09B00FF6467DAB9" modified="2017-01-18" version="1" fragment="single">LQSPKKVIKALYDYKPQGPGELEFNKGDFFHVIGNEDDPEWYEATNPSKNTRGMVPVPYFEVSNKKLSTDSMPTSRSSRSSSSNMLYAIVLYQFKAERSDELDVNIGENIILCAHHNYEWFIAKPIDRLGGPGLVPVSYVSVINILTGNSTGNDVVDDINGANLPTVDDWKNKNARYKASSIPLGNVEEDSTIPVQQEQQDGHRESRAQSQFTFENETQVDESIPYIVDASVDSFNVDNGRYWFHVNAELNNGESRSLCRYYEDFYDFQIKLLEYFPNEAGRQQQQSKRILPFIPGPLTYVTDKITKKRQVDLNDYVKELISLPKYISQSNYVKKLFETRHEYDHLIDSDNQFVQLERNKVVNSGYYEDEDPNSTIVKDESVKTLNAESGYKINHQQQGTPVKLKFYYRDDIFALMINKDVLLDELREKIRTRIYDDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P080</accession>
    <name>A0A1E3P080_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_32881</name>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037881.1">
        <property type="nucleotide sequence ID" value="XM_019182013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P080"/>
    <dbReference type="STRING" id="683960.A0A1E3P080"/>
    <dbReference type="GeneID" id="30199259"/>
    <dbReference type="OrthoDB" id="4078873at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015343">
        <property type="term" value="F:siderophore-iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF87">
        <property type="entry name" value="SIDEROPHORE IRON TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="226"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="429"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="455"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="496"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="571"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="68"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="633" mass="70095" checksum="5A7A1F1C3CDBCC09" modified="2017-01-18" version="1">MSNKKEDFNQIVEPELTNSEKKQVLDQTLDKPSSTSSIKEQTTNSTDLDQDLENQNKQVYETAWPKKLLYIAYAGLIFTALVENFAADSTKNLDSYATSSFNAHSLIATAAVVYKISAICAYPILAKLADLLGRGEGFGLAIFLYTVAYILYAACGNVETYIIAEIFYAIGRNGYRVFQQIFIADTTSLINRGIWSQLPGALTAVPSLYAGSEVMDAFLEHSTWRWGYGCFAIIIGVSMIPLTLIMTILDKRAKKLGQRKEIQVLKNLPQGSIWKKTYHLLFVELDVLGGVLLIVGFALFFIPFTLTGKQSSYRWHEGKLIAMLVIGFITFWCFVAWNALYKHKPIPNRKPFVPPQSLTNKTVIIVLVMVALDLCENSSFATYFSTVLQVGGYYTAGEATRIDNSKKATIDIASVICGLLMKYTKRSKIYVMIGVPFLVLGHGLLVYFMNRDGKLEKTVLLIVMEVFIGLGRGFYQCGLQVTIQAIAGVDGIAMSTAFFMAFNSVGSLIGSAIAGGIWNSVLLNKLEKYLPTDSKSKATSIYKSIKVAKSFKKGTPERIAISKAYRETIQIIGYTGLGIIAPMLILMFLIKEVKLTDKRDIYEGDNVEDEAEGVQVPVNNTTIPATEPMVIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P082</accession>
    <name>A0A1E3P082_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[RNA-polymerase]-subunit kinase</fullName>
            <ecNumber evidence="2">2.7.11.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_84418</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.23" evidence="2"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037857.1">
        <property type="nucleotide sequence ID" value="XM_019185707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P082"/>
    <dbReference type="STRING" id="683960.A0A1E3P082"/>
    <dbReference type="GeneID" id="30202953"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070985">
        <property type="term" value="C:transcription factor TFIIK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140836">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat S5 kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905866">
        <property type="term" value="P:positive regulation of Atg1/ULK1 kinase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07841">
        <property type="entry name" value="STKc_CDK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000097">
        <property type="entry name" value="Putative cyclin-dependent kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000498">
        <property type="entry name" value="Serine/threonine-protein kinase KIN28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037770">
        <property type="entry name" value="CDK7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013516">
        <property type="entry name" value="Phyto_chromo_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF0">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00245">
        <property type="entry name" value="PHYTOCHROME_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="15"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="21"/>
            <end position="29"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 11">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR637770-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR637770-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ58650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="320" mass="36333" checksum="9518953618422A83" modified="2017-01-18" version="1">MLETNNKSSSSVQRYTKSKKVGEGTYAVVYLGKQIESGRDIAIKEIKTGQFKDGLDMSALREVKYLQELRHVNVIELVDVFSASDNLNLVLEFLPADLEMIIKDREILFSPADMKSWLLMTLRGVHHCHRNYILHRDLKPNNLLLAPDGQLKIADFGLARSLGSPNEDLTSNVVTRWYRAPELLFGAKHYTEAVDLWSVGIIFAELMLRVPYLPGRDDLDQIDVTFKALGTPTEENWPNVSSLPLYNNLNFYPCPSRQEIRNRFLAATETALDLMCGMTELNPAKRWNTEKALLSKYFTDLPAPTEPAKLPKLTKKESSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0C4</accession>
    <name>A0A1E3P0C4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_33960</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037599.1">
        <property type="nucleotide sequence ID" value="XM_019182134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0C4"/>
    <dbReference type="STRING" id="683960.A0A1E3P0C4"/>
    <dbReference type="GeneID" id="30199380"/>
    <dbReference type="OrthoDB" id="71302at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11398">
        <property type="entry name" value="bHLHzip_scCBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047206">
        <property type="entry name" value="bHLHzip_scCBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47787">
        <property type="entry name" value="CENTROMERE-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47787:SF1">
        <property type="entry name" value="CENTROMERE-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="5">
        <location>
            <begin position="8"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="110" mass="13091" checksum="363B459963891D03" modified="2017-01-18" version="1" fragment="single">GTEEWHRIRKDNHKEVERRRRENINTGIKELASLLPTQDSNKSQILQRAIEYIKRLKENENNNIEKWTLEKLLTDQAVAELTASNEKLKAELERAYREVEHWKKVSVGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0J1</accession>
    <name>A0A1E3P0J1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_96217</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037649.1">
        <property type="nucleotide sequence ID" value="XM_019186513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0J1"/>
    <dbReference type="STRING" id="683960.A0A1E3P0J1"/>
    <dbReference type="GeneID" id="30203759"/>
    <dbReference type="OrthoDB" id="432483at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097035">
        <property type="term" value="P:regulation of membrane lipid distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000749">
        <property type="term" value="P:response to pheromone triggering conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05574">
        <property type="entry name" value="STKc_phototropin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000121">
        <property type="entry name" value="Serine/threonine-protein kinase nrc-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000078">
        <property type="entry name" value="Serine/threonine-protein kinase nrc-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45637">
        <property type="entry name" value="FLIPPASE KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="416"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="328"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="79"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="122"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="146"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="203"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="272"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="335"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ58442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="812" mass="91457" checksum="1A3F7ED5AF5FA6B4" modified="2017-01-18" version="1">MTSTALRENNRNLFLSHDSISQKGSPSPAPAEDFSSSSSRNVETVPQSNNESTQNLQPPTPPLSKLRSRSMSLGKIFNFGSNKADQNDSNGQPLEQPHEAKIHEPLSPSVEPISDNQFHQPTDTEKPLDPTHEEKKLTTKIKSFFKINSNSATTSPNLTSTKPEIKINNDDDNEEDEDFKLENPSKIHESNLHHEYPIKETLDPPTETLEEQPQQTPTSKTSSGGSKFAQAARRLRSPSSPNPPNNNKKEASSNNNFITSQPYFAHQGLPPHGDDEIHKEIIDNSSSGKGHGPIPFALKKNNTNSQSSLNQIIEEFDQRLNSFEIPENQKEEEVQQQSEAQQQQQQSNGQPKPPLRRVASAPLGLKNDSSSNEHTHSDSDKKLEEITESKTRPRKKSFLSSNAIKVSELQVGPQSFEKIRLLGKGDVGKVYLVREKETNRLYAMKVLSKKEMIQRNKIKRALAEQEILATSNHPFIVTLYHSFQSEDYLYLCMEYCMGGEFFRALQTRKSKCIPELDAKFYASEVVAALEYLHLMGFIYRDLKPENILLHQSGHIMLSDFDLSKQTESIKNPSMSFNNNRNYQTLDTKICIDGFRTNSFVGTEEYIAPEVIRGKGHTVAVDWWTLGILIFEMLFGTTPFKGANRNQTFSNILKSDVSFPDTQLISSNCKNLIKKLLIKDENKRLGSKLGASDIKNHVFFKNTQWALLRNQKPPMIPVLSKNGTDFHKPNKNNKQSSIDISNEQILKEVSDQEDSNVDDPFGDFNSMTLIHEGNNDDDSLIYGTGNSYGSISYTVTSGNRPRTNSKSIFKTKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0K5</accession>
    <name>A0A1E3P0K5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_63467</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FMO family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038168.1">
        <property type="nucleotide sequence ID" value="XM_019183553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0K5"/>
    <dbReference type="STRING" id="683960.A0A1E3P0K5"/>
    <dbReference type="GeneID" id="30200799"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000138">
        <property type="entry name" value="Flavin-containing monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00370">
        <property type="entry name" value="FMOXYGENASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="452" mass="51273" checksum="9CE2B8B33B8359A4" modified="2017-01-18" version="1">MTKRYSRIAIIGGGPVGLGVAKALGLEKFPQIDLFEKNSKFGGLWNYEGPTTTRPSIPSVHHSDPEFNNDEKSPMYKYLETNITKEIMQYKDFPFPEECETFPTRQEVLQYIQNYGETLSTDVNVLFNSNVLSVEKIDDVWKVEVTIDGGNIETREYDAVVLANGHYNQPYIPDVEGLKQWNKLEAGSITHAKYFNESLDFKDKTVLVIGNSASGLDIALQLSTVAKKVYNSVRTPSPVADIKIHGVEEIGEIVKYDYLNSKTVVTKDGAHYKDIDAVIFCTGYLYKFPFLKSYLSGDDALLTNGERVRRLYKHLFYIPDPSLVCVGLPKSVIVMPFAECQGAFIARVLSGRLRLPSEKEQREEELKEIEARGEGKEFHNIKPPLDTAYCQELQKIIDDQGLNEGFEADRWGDYRVGLRKESGPAKARRYEQIVEHANSLRENNEPYRLLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0U3</accession>
    <name>A0A1E3P0U3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Glycoside hydrolase family 132 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_84720</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SUN family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038268.1">
        <property type="nucleotide sequence ID" value="XM_019185762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0U3"/>
    <dbReference type="STRING" id="683960.A0A1E3P0U3"/>
    <dbReference type="GeneID" id="30203008"/>
    <dbReference type="OrthoDB" id="5339822at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051526">
        <property type="entry name" value="Beta-Glucosidase_SUN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005556">
        <property type="entry name" value="SUN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31316">
        <property type="entry name" value="BETA-GLUCOSIDASE-LIKE PROTEIN NCA3, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31316:SF0">
        <property type="entry name" value="SECRETED BETA-GLUCOSIDASE SIM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03856">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133620" description="Glycoside hydrolase family 132 protein" evidence="12">
        <location>
            <begin position="22"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="70"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="92"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="419" mass="43179" checksum="5DEFF4A6621CBEBA" modified="2017-01-18" version="1">MKFSTSFVAAYAAYLMGSAVAAPHAHAGVAKRDAECATTAHHHHQHKRAVAVEYVYSTVTVDGQGQTVSASASQTTLEPSSVVDRAASVKPSSSSSSTSTSTSAAPTSTSASSSEAQSTTSSSTSSSSSSSSSSGGSSGSSGSIYGDLSDFSGPNKEFQDDTISCTDFDSLVGQGVISLEHLGFGGFSGIYHPSDTSTGGKCTEGAYCSYACQPGMSKTQWPSEQPGNGVSVGGLVCKNGKLRRSNTNQKYLCEWGVDAAYVVNNLSQNVAICRTDYPGTENMVIPTNVEGGKSLPLTVVDQSTYYQWGGKGTSAQYYVNNAGVSWEDGCVWGSAGSGIGNWAPLNFGAGAVNGISYLSLIPNPNNKESLNFKVKIVASDDNSNVSGECTYANGQYNGNGNDGCTVGVTKGSAKFVLYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P184</accession>
    <name>A0A1E3P184_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ergosterol biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_63740</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERG28 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038453.1">
        <property type="nucleotide sequence ID" value="XM_019183603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P184"/>
    <dbReference type="STRING" id="683960.A0A1E3P184"/>
    <dbReference type="GeneID" id="30200849"/>
    <dbReference type="OrthoDB" id="6485510at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005352">
        <property type="entry name" value="Erg28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15451:SF19">
        <property type="entry name" value="ERGOSTEROL BIOSYNTHETIC PROTEIN 28 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15451">
        <property type="entry name" value="ERGOSTEROL BIOSYNTHETIC PROTEIN 28-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03694">
        <property type="entry name" value="Erg28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="11" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="86"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ59246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="16333" checksum="FA5901175DA29AB9" modified="2017-01-18" version="1">MSSFLDLVGPWIPQTPGILPKWLLFISIVSVFNSAQTYFSGLELTRRVYENKPLETTNLSARTFGTWTFVSAVIRFYGAYYLTNPQVYQITLASYLIAFFHFNSEWLIFKTAKFGKGLLGPLIVATSTIVWMTLQKDYYMGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1I3</accession>
    <name>A0A1E3P1I3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_63761</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038472.1">
        <property type="nucleotide sequence ID" value="XM_019183605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1I3"/>
    <dbReference type="STRING" id="683960.A0A1E3P1I3"/>
    <dbReference type="GeneID" id="30200851"/>
    <dbReference type="OrthoDB" id="7608935at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071031">
        <property type="term" value="P:nuclear mRNA surveillance of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071036">
        <property type="term" value="P:nuclear polyadenylation-dependent snoRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071037">
        <property type="term" value="P:nuclear polyadenylation-dependent snRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051644">
        <property type="entry name" value="TRAMP_AT-DNA-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46543">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46543:SF1">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="9">
        <location>
            <begin position="136"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="183"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="220"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="287"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="303"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="354"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="365" mass="41338" checksum="4F95D55A1BB82574" modified="2017-01-18" version="1">MDVVEAGDTDFVKPSEGSKTISLTIDEVNDNADELVELRGEGRYFGASDTPSGPVCSNCHQRGHKKANCKTVVCMACGMVDDHYSQHCPKSIQRRVYCQTCSSTRHFSDRCTSIWRSYLTTKDSDRKMGLPTNIYCYNCAEKGHYGDECPSERTSRVPNLDGSAFSGHNLPQQLRDKYFSLHKKRRRDDDYDEDDRYNDNYSISSDSKKDQKKKNGLFNRIKDTFKRNKGSDGYDDEGPSKKKNKPSKKEIRKAQSTPNFNNRPIHKSGVLPPPAANRSSLDYPRNPSYNNGYQQGGYSRVDSYNQPYNQSYNNQSYNQGYNQGYNQGYNNGPSRSGYLEPSRSGGPGGGYKGGRFRQKNKGPRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1U1</accession>
    <name>A0A1E3P1U1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcriptional adapter 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_84588</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="ODQ58877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_019185738.1"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="GOC"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
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            <end position="111"/>
        </location>
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            <end position="107"/>
        </location>
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            <end position="153"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="10">
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        </location>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
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            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycetes</taxon>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051264">
        <property type="entry name" value="FAD-oxidored/transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43716">
        <property type="entry name" value="D-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43716:SF1">
        <property type="entry name" value="D-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="5">
        <location>
            <begin position="95"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="501" mass="55560" checksum="61034B42393A6B5D" modified="2017-01-18" version="1" fragment="single">MLRSTVKRSFSTLTRRSLLVKSSSAPLSSRIVTRNYASKIVPLTSETYPNVHRDQRYKKLSSQDLDFFKSILPESSIIQDSDDLLFYNEDWMRKYRGQSKLTLKPKTTEQVSQILKYANEHSLAIVPQGGNTGLVGGSIPVFDEIVLNVSNLNSIRSFDKTSGILKADAGVVLEIADQYLAEHGYIFPLDLGAKGSCHVGGVVATNAGGLRLLRYGSLHGSVLGLEVVLPDGRVLSSLDALRKDNTGYDLKQLFIGSEGTLGVITGVSILCPPRPQSFNVAFLGVESYEAVKTTFKSAKRELGEILSAFELMDSNSQVLAKEHLKTTHPLEDEHPFYVLIETSGSDATHDQEKLEKFLEQAFEQEIISDGVIAQDETQLKNLWNWREQIPEASTIGGGVYKYDVSLPLDHLYGLVDAVRERLIEHKLLGETEEFPVIDAIGYGHVGDGNLHLNVAVRRYTKDVEKVLEPFVYEWVSSKKGSISAEHGLGFQKKNYIGYSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2A1</accession>
    <name>A0A1E3P2A1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Stress response RCI peptide</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_91138</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019186280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2A1"/>
    <dbReference type="STRING" id="683960.A0A1E3P2A1"/>
    <dbReference type="GeneID" id="30203526"/>
    <dbReference type="OrthoDB" id="3980682at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="77"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="15722" checksum="EDA64D821991620B" modified="2017-01-18" version="1">MQFDTSDVILYVLAFFLPPFPILIRVGFCTNQFLLNVLLTLLFGLPGTLHALYIIYITSPITGSPDRRVINGDYERVIENDGTTPNNDNYPQAVFDQQADNAQQIPSSSSIHQPVNAPPPPYEDSKDFNKDSTDNKVQKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2D2</accession>
    <name>A0A1E3P2D2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_31580</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038264.1">
        <property type="nucleotide sequence ID" value="XM_019181850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2D2"/>
    <dbReference type="STRING" id="683960.A0A1E3P2D2"/>
    <dbReference type="GeneID" id="30199096"/>
    <dbReference type="OrthoDB" id="9986881at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF31">
        <property type="entry name" value="POLYAMINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="388"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="447"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="483"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="71"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="520" mass="57291" checksum="C435B4887CE46670" modified="2017-01-18" version="1">MSRVESRSEVSRDLSRALTNADSVEQDALKDTSPLPPMGLNKVIPTDYPDKEPYTVSYNGADDPLHPHNWPVMKKLITCAIIGFNTGCIAWGSSIFSAATIVVARQYHVGNVVSTLGTSLYVLGFATGPVIWAPLSELYGRRMILILSCLGFTLFQFAIATAENLQTILICRFFGGTIGAAPLVVVPASFADLFNANSRGKAITIFSMTVFCLPIIAPVAGSFIVYSYLGWRWTEYVTGIMGAAGVVLITFFYEETHHPIILVNKARELKQRTGNWLIHAPHDEFSLSFKDIVEKNLTRPIFMLFTEPIIFLITIYNAFIYGILYLLLSAYPIIFAEGYKMKGGVAELPYLGVALGMVFGGCYCYAMEGRYIKAMNANGGKPVPEARLYPMFAGAILFPVGIFWLTWTGNYHNKVHWMAPTASGVFTGFGLITIFNSSINYIIDSYLIFAASAMAANAFLRAAFACAFPLFSVAMFHNMGTNWAGLLLACVGFVLIPVPFLFFRFGKKIRGKSKYAFVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2N9</accession>
    <name>A0A1E3P2N9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_31904</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038369.1">
        <property type="nucleotide sequence ID" value="XM_019181897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2N9"/>
    <dbReference type="STRING" id="683960.A0A1E3P2N9"/>
    <dbReference type="GeneID" id="30199143"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF80">
        <property type="entry name" value="SUPPRESSOR PROTEIN MPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="32"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="68"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="115"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="152"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="190"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="233"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="272"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="428" mass="49821" checksum="84596E3052B7A291" modified="2017-01-18" version="1" fragment="single">DILKLSVDQYGCRFLQKKLDLDSSIKDVIFNKIFNNLINLIIDPFGNYLIQKLIDHLSTYQKDLMIEKIHGYLFLISINQYGTRSLQKIIDKISNTYQIDLVIKGLQMNDVPNVSIEDNNIVKLIKDLNGNHVIQKCIFKFPPEKFQFLIDSICMDNNIVRISTHKHGCCVLQKLLNNANFNQILKISNNLLIYLDDLINDQFGNYIIQFLFELNFLKSSNSINFLIDDFFNRIYGNLIQLSCLKFSSNVVEKFIKILKHKQNYVYLIEIIKFVDSNFEILIKDKFGNYVIQTLIDQFYDVVELSTEMNNLILNIKSYLPVIKSAPYARKIQLKIQQLETNYNLMSSASSINSFERNGGHGGGNFHTNSAFQFDNDQFRNPKNQYYMNYNSNPHFINDQQFISSSTQFIYPPPPTNSDFPAPNQFQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2Z9</accession>
    <name>A0A1E3P2Z9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Protein YOP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_31803</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required to generate and maintain the structure of the tubular endoplasmic reticulum network and the vacuole. Induces high curvature in membranes and causes membrane tubule formation. Involved in membrane/vesicle trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038464.1">
        <property type="nucleotide sequence ID" value="XM_019181882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2Z9"/>
    <dbReference type="STRING" id="683960.A0A1E3P2Z9"/>
    <dbReference type="GeneID" id="30199128"/>
    <dbReference type="OrthoDB" id="10009287at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180020">
        <property type="term" value="F:membrane bending activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048309">
        <property type="term" value="P:endoplasmic reticulum inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990809">
        <property type="term" value="P:endoplasmic reticulum tubular network membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032581">
        <property type="term" value="P:ER-dependent peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051292">
        <property type="term" value="P:nuclear pore complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF161">
        <property type="entry name" value="RECEPTOR EXPRESSION-ENHANCING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="180" mass="19753" checksum="309BA25DF4B74C93" modified="2017-01-18" version="1">MADFASQFQSTLKGLDSKFAGNSILTQFEKVSGLPKSYGILGSGVFYFLLIFLNIGGIGQLLSNIAGFVIPGYYSLLALQTATSKDDTELLTYWVVFAFLNVIEFWSKAILYWIPFYWFIKTVFLLYLALPQFGGAQIVYNVVIKPIAEQYITGKPSAANKFANAVDDAAEGVASGASRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P327</accession>
    <name>A0A1E3P327_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Imidazole glycerol phosphate synthase hisHF</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="12">Glutaminase</fullName>
                <ecNumber evidence="12">3.5.1.2</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="12">Cyclase</fullName>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_42228</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 12">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The glutaminase domain produces the ammonia necessary for the cyclase domain to produce IGP and AICAR from PRFAR. The ammonia is channeled to the active site of the cyclase domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">In the C-terminal section; belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="12"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039093.1">
        <property type="nucleotide sequence ID" value="XM_019182567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P327"/>
    <dbReference type="STRING" id="683960.A0A1E3P327"/>
    <dbReference type="GeneID" id="30199813"/>
    <dbReference type="OrthoDB" id="10254903at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01748">
        <property type="entry name" value="GATase1_IGP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04731">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000094">
        <property type="entry name" value="Imidazole glycerol phosphate synthase hisHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000039">
        <property type="entry name" value="Imidazole glycerol phosphate synthase hisHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00278">
        <property type="entry name" value="HisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004651">
        <property type="entry name" value="HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050064">
        <property type="entry name" value="IGPS_HisA/HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014640">
        <property type="entry name" value="IGPS_HisHF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010139">
        <property type="entry name" value="Imidazole-glycPsynth_HisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00735">
        <property type="entry name" value="hisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01855">
        <property type="entry name" value="IMP_synth_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235:SF2">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE HISHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISF/H IGP SYNTHASE SUBUNIT HISF/H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036936">
        <property type="entry name" value="IGPS_HisHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 12" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 12 15" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="3 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="16">
        <location>
            <begin position="7"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="PRFAR binding" evidence="14">
        <location>
            <begin position="362"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="PRFAR binding" evidence="14">
        <location>
            <begin position="400"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="PRFAR binding" evidence="14">
        <location>
            <begin position="472"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="PRFAR binding" evidence="14">
        <location>
            <begin position="498"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="PRFAR binding" evidence="14">
        <location>
            <begin position="521"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="13">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="13">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="13">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="402"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="14">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR036936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR036936-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR036936-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ59886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="552" mass="60198" checksum="0ECED2CC56B4DD77" modified="2017-01-18" version="1">MPEIVHIIDVESGNLRSLQNAVEYLGYETKLIKDASDPELSKATKLFLPGVGSYGHFVRQLNAKGFVKPVKEYIAQGKPIMGICIGLQALFDGSEESPDDIGLNYINFKLSKFDPTNKSVPEIGWNSVSAVGDNMLYDINPNNRYYFVHSYAAILNEKLKKDLSSNGWEIAITKYGDEEFVAAVAKDNIFATQFHPEKSGLAGLKVIRAFLEQKKHSTLPEITVPNDYSNNGLTRRVIACLDVRTNDNGDLVVTKGDQYDVREKDAQGGDVRNLGKPVELAEKYYKQGADEVTFLNITSFRNSPIADQPMLEVLKLAAKTIFVPLTVGGGIKDVVDPDGTKVPALQVAGLYFRSGADKVSIGTDAVIAAKKYYANGGKGDGTSPIETISKAYGVQAVVISVDPKRQYVKSQADTQNTTFKTKFPGPNGEEYVWYPTTIKGGRETTDLGAYELVQACEALGAGEILLNCIDKDGSNSGFDLELIDHIKSAVSIPVIASSGAGKPEHFSEVFTKTKTDAALGAGMFHRDEFTVKQVKVHLRESGLQARLDEETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P331</accession>
    <name>A0A1E3P331_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bystin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_20619</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bystin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039096.1">
        <property type="nucleotide sequence ID" value="XM_019180880.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P331"/>
    <dbReference type="STRING" id="683960.A0A1E3P331"/>
    <dbReference type="GeneID" id="30198126"/>
    <dbReference type="OrthoDB" id="2192561at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="entry name" value="Bystin"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BYSTIN"/>
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        <property type="entry name" value="Bystin"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="455" mass="52848" checksum="C5D8CB2FD32DA6C2" modified="2017-01-18" version="1" fragment="single">KPRHDPLYKELTTQGGNLRTKARGAKKVERDEEAAEDYIDSTSSRKILQLAKEQQDEVEKEENRELKAQNNFSFGSRYIEQDDEEEEEDEENYENISDFGSDVEYDEFEVEEEVDERDAALFEMFLNKQGRSAETQAFNLGDKIMATLKAKEQEHEEEEQEKTIERPKDAVLLPPKVIAAYTQVGQILSTWTHGKLPKLFKVIPSLNNWQDVLYVTNPPEWTPHVVYEATKLFVSNLQSKQAERFVEDVLLERFRSDIKESETHSLNYHIYRALKKSLYKPGAFFKGFLFPLVETGCSVREATIAGSVLSKVSIPPLHSSVALNYLLNMDFSPPTTVFIRILIEKKYALPYQIIDNCVFYFMKFRLITDTSMMDEAEERDAKKQPLLPVVWHKAFLAFAQRYKNDITDDQRDFLLETVRQRNHKDISPEIRRELLAGTARQDPSAEKEEVMTDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3H0</accession>
    <name>A0A1E3P3H0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_69774</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038634.1">
        <property type="nucleotide sequence ID" value="XM_019184181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3H0"/>
    <dbReference type="STRING" id="683960.A0A1E3P3H0"/>
    <dbReference type="GeneID" id="30201427"/>
    <dbReference type="OrthoDB" id="1742084at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037382">
        <property type="entry name" value="Rsc/polybromo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054551">
        <property type="entry name" value="RSC4_Ig-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062:SF19">
        <property type="entry name" value="PROTEIN POLYBROMO-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062">
        <property type="entry name" value="SWI/SNF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24189">
        <property type="entry name" value="Ig_RSC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="49"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="198"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="138"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="287"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="138"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="287"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="302"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="324"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="546" mass="62351" checksum="C99917B5C4183A0D" modified="2017-01-18" version="1">MAPKQRRKSGGATSPEQPAKRQKIAPANDKETLDFYSTTLTLVQDLREGKNQISFDFLKLPSKKLYPDYYQTIKSPISINEISIKVKTNQYPTTQDFLNDFKLMANNANEYNDPESFIAKNSLTILDFVEDQVKQFNAGSNKPTEVEQQTPKLPKIKIRAPAAPSSSSTPSTPATPSNAGLKKKYLEILQELIDFEVDGIRIGDPFLEEVSRKDFPDYYQLIKHPMALNSVRKHINGNRMRSLDQFIEEINLIWQNAQLYNEEESLIYQDSKTLQDYFQKRIEELKHELSQPKSNDHSTPSSKKKPVIKLKPPVKSKLNLQVKNEQEDRPIDEPAKDASNEEENKLETKEEEVTTEPENKQVIEPTTETKVEGQDQLETIKSTRKRSSKQTAADALIQEISIASSRSVYKQQVKNQNNNPIPAIFQNWFEYRFEANDFQMQSYTLSLPPQQGAVSVLAALNDSLTDRKHQANLTVNGERVSPIPSVQYADTGRRLTSRYELKLAVGLNMVVFDVAVDPIFNEESRLRSQDALPNVEKLTFWIQVIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3J4</accession>
    <name>A0A1E3P3J4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_78609</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039192.1">
        <property type="nucleotide sequence ID" value="XM_019184758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3J4"/>
    <dbReference type="STRING" id="683960.A0A1E3P3J4"/>
    <dbReference type="GeneID" id="30202004"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF33">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="105"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="694" mass="77957" checksum="A0F701CC7630076A" modified="2017-01-18" version="1">MTALPLPNADKITNLEEIYPLLPLDFSKTKTSTPVPGSQTDQYSPVFKNSYVKDGELVGYLHPKYKTFYHLFQENLKIHSQNKSIGYRVKDSKTGQFSSEFHFETYQQLAEKRDYAGSGFLHLVSEYAPNADINNFILTLFSANKPEWIVSDLGTHAYSIPNTPLYDTLGTTAIKYILNLTETPIIVLSKNKLEKILNVDSKFLKILITIEELDPTEDAILIKDAESKGYKILDFKKLLAIGKENLKPHRVPTPDTLYTISFTSGTTGIPKGVELKHSHVMASGSFLFIHVTFPKNPVSLVLLPLAHVYERSKIIYELGKGAAIAFPSNPENPKTFLDDIKILKPSHISAVPRLYSRIESGIKEKIKSTPGFKGAILRFAVNYKLNHNDDEFLVKILDSLIINKIKQQVGFGNLDFLISGGSPLAGESIYYLRKVLNSGFYQGYGSSETFGGIAITTKFNKDTSRCGSIGVTSEFKLRNLPEMNYTFDSNRSGELILRGTQVFNTYYKNPEATSDSVDSEGWFSTGDIAQVDEFGSIAIIDRVKNFFKLAQGEYIASEKIENFYSGNNSFINQIFVYGDSFQSYLIGIIGIEEPMLLKLIKNSSSLGSKISTHEQLLAKLNDLEVRKFVLKELNNNIEHTGLYSFEKLKNIHLAIEPFNTEDDTITPTLKLKRPNAKKKFQTTIDELYKEGPIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P413</accession>
    <name>A0A1E3P413_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_78660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039245.1">
        <property type="nucleotide sequence ID" value="XM_019184768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P413"/>
    <dbReference type="GeneID" id="30202014"/>
    <dbReference type="OrthoDB" id="4093852at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133668" evidence="1">
        <location>
            <begin position="19"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="185" mass="19663" checksum="0B9974C1DC10CCD0" modified="2017-01-18" version="1">MNPQIWLLALLVPSVVNAISDSFTLELSTKENGLKNLQIMETDEDIFIGQSSDYFQVLNGTIQDDGSLKLGNNSFIGISKNLFTVTNDTTEYAQPFSIDKKGKLNLYGSKKFKAIPSGGTGSARWTLGSAQATSNLKEGVYEVEIKCVNSKGKSANKFSIPGSGASGHLDPISGLIAAGIVIGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P466</accession>
    <name>A0A1E3P466_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thiamine pyrophosphokinase</fullName>
            <ecNumber evidence="7">2.7.6.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_62706</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>thiamine + ATP = thiamine diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11576"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18385"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the thiamine pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.2" evidence="7"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039350.1">
        <property type="nucleotide sequence ID" value="XM_019183422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P466"/>
    <dbReference type="STRING" id="683960.A0A1E3P466"/>
    <dbReference type="GeneID" id="30200668"/>
    <dbReference type="OrthoDB" id="25149at2759"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00597"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030975">
        <property type="term" value="F:thiamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07995">
        <property type="entry name" value="TPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10240">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.320">
        <property type="entry name" value="Thiamin pyrophosphokinase, thiamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006282">
        <property type="entry name" value="Thi_PPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016966">
        <property type="entry name" value="Thiamin_pyrophosphokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007373">
        <property type="entry name" value="Thiamin_PyroPKinase_B1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036371">
        <property type="entry name" value="TPK_B1-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007371">
        <property type="entry name" value="TPK_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01378">
        <property type="entry name" value="thi_PPkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622:SF8">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04265">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04263">
        <property type="entry name" value="TPK_catalytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031057">
        <property type="entry name" value="Thiamin_pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00983">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63862">
        <property type="entry name" value="Thiamin pyrophosphokinase, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamin pyrophosphokinase thiamin-binding" evidence="8">
        <location>
            <begin position="222"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR031057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="303" mass="33795" checksum="C661ECFB19E5A00B" modified="2017-01-18" version="1">MSENTVPLEEMTNITENPESIEVSCPAGANIISLTSALKPTSRSALLILNQPIVIPRFKALWDNFQLKICGDGGANQLFRLFTTDEERKLYLPHYIIGDLDSLENDVRSWYESHGVEVIPQTTQYATDLNKCLDAVEVYYNLKDLNQEFVAKEIDHYDGLIKLHSNIKSHNAIQCVLLGAIDGRFDHTIQSISLLFNLSKTNPNLRLYYLSPTDLIFMIPKGVNHLEYENLSAIHGSNCGLLPLGGPVKLTTHGFKWDVANWESSIKGSVSSSNRLVGVNGATIESDDDLILNIQVNHQELSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4G1</accession>
    <name>A0A1E3P4G1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_84255</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039586.1">
        <property type="nucleotide sequence ID" value="XM_019185679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4G1"/>
    <dbReference type="STRING" id="683960.A0A1E3P4G1"/>
    <dbReference type="GeneID" id="30202925"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF18">
        <property type="entry name" value="AMINO ACID PERMEASE_ SLC12A DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="384"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="455"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="486"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="59"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="553" mass="61043" checksum="0B6FBD4DF6F93DA5" modified="2017-01-18" version="1">MNHAQKKSEAYQEGMKVLCDQHDSAEKGNHVEVSVDDSDSSSVFGEDRHRLKSTLEQRHIQMLALVGVFGTGIFLSSGSALATTGPAGMLIGYSLIAFIVGLNQVALAEVASLMPVSSATVRHLEQFIDPAWGFAYGWISIWGSIMPGEISAAAVIISYWTDISQAAWISIIIFVIIATNSYTVRFYGEVEFVFALIKISLLIGLIIVSIVITSGGGPTHETIGFRFWRNPGPFKESITTGNLGKFAAFWKTLSGVVYSFGGVQSVPALAAEVKNPRRTVFTACKRIFYRVTFLMITTVFCLTLIVSSNDKRIANSSGNAQSSPFVVAIRNANIKVLPHIINAAVLTSAFSAANLSIVHGSRTLFALAVKHQAPRVFLRTNKRGLPWVGVIFIAAFMPLAYMNVSTNAANVFNWFQSLTSANLLLGWILISANHIHLSRAMKAQGISRDSLPHKFKFGVQAAWFSGIASLILLLTGGFKNFVHGDFLISSFFSSYFIIPLSWGLFFFWKFFKKTRYYRPHEVVLTPLFKDVEENPEEPEPKVRGWKIITLLWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4H9</accession>
    <name>A0A1E3P4H9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_68886</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the citrate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039603.1">
        <property type="nucleotide sequence ID" value="XM_019184142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4H9"/>
    <dbReference type="STRING" id="683960.A0A1E3P4H9"/>
    <dbReference type="GeneID" id="30201388"/>
    <dbReference type="OrthoDB" id="8017587at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046912">
        <property type="term" value="F:acyltransferase activity, acyl groups converted into alkyl on transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006101">
        <property type="term" value="P:citrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.230.10:FF:000001">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.580.10:FF:000001">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010109">
        <property type="entry name" value="Citrate_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01793">
        <property type="entry name" value="cit_synth_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007128">
        <property type="entry name" value="PRK09569.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739:SF8">
        <property type="entry name" value="CITRATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="3">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="351"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR610109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="471" mass="52419" checksum="B2C4ECC2E2901A95" modified="2017-01-18" version="1">MSSLRLASQTLRSNSSLLRKNSILLATRSYSSGEKTLKQRFAELIPEKVEEIKQLKKDYGKTVIGELTLDQAYGGMRGIKGLVWEGSVLDPVEGIRFRGRTIPDIQKELPKAEGGEEPLPEALFWLLLTGEVPTESQVKTLSAELAARSQLPKHVEELIDRSPSHLHPMAQFSIAVTALESESQFAKAYASGVSKKDYWNYTYEDSIELLAKLPNIAARIYQNVFKDGKLPQVNKDLDYGANLANLLGYGSNQEFVELMRLYLTIHSDHEGGNVSAHTTHLVGSALSSPFLSLAAGLNGLAGPLHGRANQEVLEWLFKLKKEVKGDYSKETIEKYLWDTLNAGRVVPGYGHAVLRKTDPRYTAQREFALKHLPDFDLFKLVSTIYEVAPQVLTKHGKTKNPWPNVDSHSGVLLQYYGLTEESFYTVLFGVSRAFGVLPQLIIDRAVGAPIERPKSFSTAKYIELVKGLSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4R3</accession>
    <name>A0A1E3P4R3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_78819</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039411.1">
        <property type="nucleotide sequence ID" value="XM_019184790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4R3"/>
    <dbReference type="GeneID" id="30202036"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="667" mass="78018" checksum="7950B786E43C9381" modified="2017-01-18" version="1">MPGSAEPHLMNRYYDFVESALIGASTRSLQNFEFQEFWSSNRAQSVTQQTIYDKISEIKDILIRGSDTTNQPLLHQRFAFRTFFIFPDISVLQCLRFTPLDNGNRYVLYKNKIPDLIFNKAISVAHFSSSEIPDSFFGRVFNSMSDRFEKTITEGFPDPVGRPSARFEDAITERTRGHYRGMFEYFSEHHGINADTLGNKQFKDRARIYLERACLRPIMFMFKGFFKVEKMFECEPLKLLKETVDICDRRGKVVHSFPWELSSLDMERKDGQVFRLPILFVDSCTSQLYSDICFDPILKKNIRNALVSMIATNTPRCIIIDCSQMFYFELDAIDNEIEVNQKEVRLPLRYKFMDIDTAGLTATMVIASLIYHGYFIKRDTDFVKDKRIMADAKKLFMRNYHELTFEIKKFSDEIYLDQEIQEHHQRFKRVIFDTKRHGKLNYSGENFDSRKSFQTIRMSYEQYQKTFQITTPLDDRGKHGVILRIFDPIRRIIGGQFLDVVDSPRAMFELCQQEYQDTLTAFKEITSYNNTTSDIDAKINLPRVHENGNIVFSTRNRKKFFCGAYIVIEEIKGQIKTPEEMSLASKQLDLMHNLFMVFDGQLELEDIVVQDGKVFFTNFASCFILYAGCLPYMEEDIRRLRKFRKEILGEDENSNISSPLDASPSVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4U4</accession>
    <name>A0A1E3P4U4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Fatty acid synthase subunit alpha</fullName>
            <ecNumber evidence="18">2.3.1.86</ecNumber>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="18">3-oxoacyl-[acyl-carrier-protein] reductase</fullName>
                <ecNumber evidence="18">1.1.1.100</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="18">3-oxoacyl-[acyl-carrier-protein] synthase</fullName>
                <ecNumber evidence="18">2.3.1.41</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="26" type="ORF">WICANDRAFT_54108</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 18">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>acetyl-CoA + n malonyl-CoA + 2n NADPH + 4n H(+) = a long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP(+).</text>
            <dbReference type="EC" id="2.3.1.86"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 18">Belongs to the thiolase-like superfamily. Fungal fatty acid synthetase subunit alpha family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.86" evidence="18"/>
    <dbReference type="EC" id="1.1.1.100" evidence="18"/>
    <dbReference type="EC" id="2.3.1.41" evidence="18"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039507.1">
        <property type="nucleotide sequence ID" value="XM_019182732.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P4U4"/>
    <dbReference type="GeneID" id="30199978"/>
    <dbReference type="OrthoDB" id="4251012at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005835">
        <property type="term" value="C:fatty acid synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004321">
        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101026">
        <property type="term" value="P:mitotic nuclear membrane biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900535">
        <property type="term" value="P:palmitic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00828">
        <property type="entry name" value="elong_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08950">
        <property type="entry name" value="KR_fFAS_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.20:FF:000005">
        <property type="entry name" value="Fatty acid synthase alpha subunit FasA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2490:FF:000001">
        <property type="entry name" value="Fatty acid synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.25.70:FF:000001">
        <property type="entry name" value="Fatty acid synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="entry name" value="4'-phosphopantetheinyl transferase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040899">
        <property type="entry name" value="Fas_alpha_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047224">
        <property type="entry name" value="FAS_alpha_su_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026025">
        <property type="entry name" value="FAS_alpha_yeast"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041550">
        <property type="entry name" value="FASI_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050830">
        <property type="entry name" value="Fungal_FAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004568">
        <property type="entry name" value="Ppantetheine-prot_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00556">
        <property type="entry name" value="pantethn_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982:SF21">
        <property type="entry name" value="FATTY ACID SYNTHASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982">
        <property type="entry name" value="MALONYL COA-ACYL CARRIER PROTEIN TRANSACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18325">
        <property type="entry name" value="Fas_alpha_ACP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18314">
        <property type="entry name" value="FAS_I_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000454">
        <property type="entry name" value="FAS_yeast_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="4'-phosphopantetheinyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="22" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 20" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 18 21" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carrier" evidence="24">
        <location>
            <begin position="147"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="25">
        <location>
            <begin position="1115"/>
            <end position="1650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="101"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="352"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="101"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="active site" description="For beta-ketoacyl synthase activity" evidence="19">
        <location>
            <position position="1298"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="1764"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="1765"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="1766"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="1862"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="1863"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="21">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="ODQ60300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1875" mass="205564" checksum="DBE9E382ABE93423" modified="2017-01-18" version="1">MRPEVEQELAHVLLTELLAYQFASPVRWIETQDVFLKDYNTERVVEIGPSPTLAGMASRTIKGKYESYDAALSLQRQVLCYAKDAKEIYYTPDPADLAAKEAASAPAATESTPAATPAASGAPAAASAPAPAAAPSGPVAEIPDEPVKASLLLHVLVAQKLKKSLDQVPMGKAIKDLVNGKSTIQNEILGDLGKEFGSTPEKPEETPLEELAETFQDTFAGALGKQSSSLIARLMSSKMPGGFSVSSARKYLQSRWGLGAGRQDSVLLICLTQEPASRLAAEADAKAYLDGVAQKYASIANISLSSAAAASSSGGSGGATIDAAAFEELTKDNKILARQQLQVLARYLKLDLNKSNKLVDQQKDNLKALQDQLDNLTNELGEYFIEGITSSFSRKKARVYDSSWNWSRQSLLTLYFEIIHGCLKRVDRELVSEAINIMNRSNDTLIKFMEYHINKADATKGENYALAKELGTQLIENCKQVLNLDPVYKDISYPLGPKTNVDHKGNVKYSEEPRENVRKLQQYVHEMSQGSEVTKIPQPTIEQDLTKVYNAISKQAELSSGTKVEFEKLYGELIKFLESSSEIEIKKNTTIAGMNDDNLDSDSTKEVASLPTTSSISNPVSKVIPPETIPFLHLKKRLPDDSWTYDRSLTSVYLDGLEQAAVNGVTFKGKHVLITGAGAGSIGAEILKGLIQGGANVVLTTSRFSKKVTEYYQEIYTNYGAKGSSLIVVPFNQGSKQDVEALVDYIYDDVKKGGLGWDLDAIIPFAAIPENGIEMNQIDSKSEFAHRIMLTNLLRILGSVKNHKFERGIETRPAQVILPMSPNHGTFGADGLYSESKLSLETLFNRWHSESWANQLTICGAVIGWTRGTGLMSANNIISEGIEKLGVRTFSQSEMGFNLLGLLTPEITELCQKSPVLADLNGGLQFISSLKERTNKLRSEILETSDIRRAVSIETALEHKVVNGANADAPFAKTQINPRANMKFDFPTLKPYEEIKKLSPNLEGLLNLEKVVVVTGFSEVGPWGSSRTRWEMEAFGEFSLEGCIEIAWIMGLIKYHNGNLKGRPYTGWVDAKTNEPVEDKDVKGKYEEFILDHCGIRLIEPELFNGYNPEKKEFIQEVVIEEDLAEFEASKETAEHFKHEHGDKVEIQENPETGEFTVKLLKGASLYIPKALRFDRLVAGQIPTGWDARRYGIPEDTISQVDPITLFVLVATVEAFISAGIKDPYELYKYIHVSEVGNCSGSGMGGVSALRGMFKDRYKDQPVQNDILQESFINTMSAWVNMLLLSSSGPIKTPVGACATAVESLDIGVETILSGKAKVCIVGGYDDFQEEGSYEFGNMNATSNSLDEFEHGRTPGEMSRPATTTRNGFMEAQGSGIQVIMNADLALKMGVPIYGILGMTATATDKIGRSVPAPGKGILTTAREHHGDLKFKSSLLDISYRKRQVERRVKEVKQWIDSEYEFLQEEAKELSADEQNTYIAERIQEIQNEAQSQIKQAKDYYGNFFYQNNPRIAPIRGALATFGLTVDDIDVCSLHGTSTKANDKNESATLDMMFRHLGRSEGNPALAVFQKYLTGHPKGAAGAWMFNGALQILNSGIVPGNRNADNIDKVLEDFDYLLFPSRSIRTDGVKAVSVTSFGFGQKGAQAIAINSDYLFAVLSKEEYETYAKKFNERHNKAYSHFHKSFINNSLFKAKDKAPYDDDLQNPVYLDPLARVAPAKSGNLEYASKSIQSAESFVSASSKDTSKVLGELSSKVAGSKNVGVDVELISSINVENETFIERNFTDAEIKYCQGAPSPQSSYAGTWSAKEAVFKALGVESKGAGAQLKDIEIIRDSNGAPQVELHGEALKAAPKGSTIKVSISHDDFQSVAVAILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4Y2</accession>
    <name>A0A1E3P4Y2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_92581</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039501.1">
        <property type="nucleotide sequence ID" value="XM_019186371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4Y2"/>
    <dbReference type="STRING" id="683960.A0A1E3P4Y2"/>
    <dbReference type="GeneID" id="30203617"/>
    <dbReference type="OrthoDB" id="5572108at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10206">
        <property type="entry name" value="ASKHA_NBD_Arp8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="116"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="135"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="624" mass="71623" checksum="DA0EB12A85E18032" modified="2017-01-18" version="1" fragment="single">MSETPQPLSPTADAETPLTGSPAPTSENPKKKNSQAGKKVSAEILERRRVGRLKAAETMARKIKKSGIEKRENALKFSTFESGNLINQKNYFTDYLKKDDQIFILRERKQIRLNKVQMKQGKSVKDQDTPIPGNADEDDEMDDDEAEEIDERVGSKTIVIHPGSRNIRIGLGSDVYPQTFPFVLAIPKDDEFNKQNNYELDEEQQSIIEDNRKLLQRDFKERMRYYKRRILPNSNEIVTNFNKKVTPETIPEHNDIHKTEYFKPTANDRCFIADEALRLDSTDFKLRYPLLTNGRFNEDEYRSFAETLGDIQLFISKTLEKKFQVTKLKQYKAVLVIPDLYDKVYVENFIELLLQMSFQGVAVIQESLASTYGAGVSSACVIDIGAKTTKVSCIDEGMIIPNSRANLSYGGDDITRLFYKLLKESNFPIDYDVNNDFDWPALNQLKEKFITFQDANVTVQLYNFIKRYPSKLSEKYEFKVFDEVMLAPMGLFFPKVFEKVKDFESTPFTNRKSLDVYNGQSNNPTSTTQKNCFENNSYADKIDFEVLKDLLNPDTLEDAKEDEVPTIAALDKVIIQSIANASRYDFSKAKTFYENLLIVGGGAKIEALDFILTDRINIWRPKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P565</accession>
    <name>A0A1E3P565_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_78984</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039591.1">
        <property type="nucleotide sequence ID" value="XM_019184816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P565"/>
    <dbReference type="STRING" id="683960.A0A1E3P565"/>
    <dbReference type="GeneID" id="30202062"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000065">
        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF36">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G08340)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="422"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="464"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="60"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="527" mass="59136" checksum="6D31888431E02357" modified="2017-01-18" version="1">MTDKPTSVLQNEVTSATIEESYEEPKKSKLLDAFRAIFIWYPSNYDTEEKKLLFKLDISILVYACTSYFAKTLDTKNVTNAYVSGMKEDLLMNGNELNWLVIVHQMGYVLGQIPELLLLSRPRFSKWVLPSLEIVYGALTLLQSTVKTTNHLYAIRFFVGLAEAPTYAGVMYVLGKFYSSKSFRGNPPELYVRTATFFFGLNGGNLVSGHLQAAAHKHLSGVHGHTGWQWLFLIDGIITLGLSVVGFFLWTGIPEAGKPILLTDKEYDLVFKRLKRFDINEAKPLTVDTFKRALSDWKWYLFCLNYTIMVIVWYPIGYFSLWLKAKGNYSVSQINRYPTGCDAIGLVVSFIGTTVAGVYPPWIIYTIGALGPFVGSIILSVYHVPDPAIFAAFYISYLINITSPILYSQVNLILRKDSELKALVFGSMMTFAQFVYVWLAFALYPTTAKNPARAAPQWNIGWPVTAALSALMIVTFVLIILLHKREVRNGTAFKNPDNSDTSSEIDADATQSITSKNATKDVVKLIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5C4</accession>
    <name>A0A1E3P5C4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_84005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039234.1">
        <property type="nucleotide sequence ID" value="XM_019185633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5C4"/>
    <dbReference type="GeneID" id="30202879"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="58" mass="7014" checksum="485A24092D294D5A" modified="2017-01-18" version="1">MVEKTVWFTNWMNWLEYLHSSQIPKNTNLSKRRAHAFHYKSSRHINASRSGKELTCYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5E1</accession>
    <name>A0A1E3P5E1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TATA-box-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_77273</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBP family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039800.1">
        <property type="nucleotide sequence ID" value="XM_019184528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5E1"/>
    <dbReference type="STRING" id="683960.A0A1E3P5E1"/>
    <dbReference type="GeneID" id="30201774"/>
    <dbReference type="OrthoDB" id="2127950at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000120">
        <property type="term" value="C:RNA polymerase I transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097718">
        <property type="term" value="F:disordered domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008301">
        <property type="term" value="F:DNA binding, bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001179">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000979">
        <property type="term" value="F:RNA polymerase II core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001092">
        <property type="term" value="F:TFIIA-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001188">
        <property type="term" value="P:RNA polymerase I preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070898">
        <property type="term" value="P:RNA polymerase III preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04516">
        <property type="entry name" value="TBP_eukaryotes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000001">
        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000002">
        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033710">
        <property type="entry name" value="TBP_eukaryotic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="25891" checksum="D9BD19B20E75614D" modified="2017-01-18" version="1">MDSFKTPQTAADAKAFTSSNSFAYPTASQDLSEIKQDPSLVAEPVNGDAEDSTTSGIVPTLQNIVATVNLDCRLDLKTIALHARNAEYNPKRFAAVIMRIREPKTTALIFASGKMVVTGAKSEDDSKLASRKYARIIQKLGFNAKFTDFKIQNIVGSCDVKFPIRLEGLAFSHGPFSSYEPELFPGLIYRMVKPKIVLLIFVSGKIVLTGAKQREEIYQAFEAIYPVLNEFRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5L5</accession>
    <name>A0A1E3P5L5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="3 15">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_28273</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="15">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="3 15"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039953.1">
        <property type="nucleotide sequence ID" value="XM_019181467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5L5"/>
    <dbReference type="STRING" id="683960.A0A1E3P5L5"/>
    <dbReference type="GeneID" id="30198713"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071507">
        <property type="term" value="P:pheromone response MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07849">
        <property type="entry name" value="STKc_ERK1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000206">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000073">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0574">Periplasm</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="14"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ60746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="356" mass="41197" checksum="CA3BCA83A9D05B15" modified="2017-01-18" version="1">MGQKRITFNISTSFEVQQLLGEGAYGVVCLALHKPTGTKVAIKKIQPFERSLFCVRTLREIKLLKHFKHENIISIFDIQKPGSFEDFKEVYIIQELMQTDLHRAIISQRLTDDHVQYFIYQILRGLKALHSANVIHRDLKPSNLLLNANCDLKICDFGLARIDEQKQGNYANSPENKGISMMTEYVATRWYRAPEIMLTASKYTTAIDVWSVGCILAELLMGNPLFPGKDYRDQLIMIFKVLGTPTGEDYHSIRSRRAKEYIRSLPFYKKVPFQKILPYVNPLAIDLLSRMLTFNPSERITVVEALEHPYLATYHDPTDEPEAPPIPQEFFHFDKMKDQLSVSELKKMLYDEIMSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5P5</accession>
    <name>A0A1E3P5P5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP-dependent RNA helicase ROK1</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">ATP-dependent RNA helicase rok1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">WICANDRAFT_77280</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DDX52/ROK1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039807.1">
        <property type="nucleotide sequence ID" value="XM_019184530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5P5"/>
    <dbReference type="STRING" id="683960.A0A1E3P5P5"/>
    <dbReference type="GeneID" id="30201776"/>
    <dbReference type="OrthoDB" id="360161at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048254">
        <property type="term" value="P:snoRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17957">
        <property type="entry name" value="DEADc_DDX52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000759">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044764">
        <property type="entry name" value="DDX52/Rok1_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF15">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="19">
        <location>
            <begin position="123"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="154"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="341"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="63"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="510"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="14">
        <location>
            <begin position="123"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="550"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ60600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="562" mass="63842" checksum="30C30D338B4B9D1D" modified="2017-01-18" version="1">MDIFRVLSRGASLKKSKDIVTDYALPSEKQNASQKKKEQNMNKEVEKEIDFFRTRKFHDLAKSEDDVAKEEEISKKHHEIDQEQVEPPPPKISNEEDAKILRKSYRANVTGDDIPLPIGSFEDLISRFEFDSRLLTNLIDSGFTEPTPIQSEAIPITLFDRDVLACAPTGSGKTLAFVIPMIQQLLQSNKEQNNGLRGLIISPTKELAQQIFDECNKLVKKMPLKIAILSKSLSSKLKNNIIKKDKFDIIISTPLRLIDLVKNEALDLSKVEHLIFDEADKLFDKNFLEQTDDILTQCKNPSLRKSMFSATIPSSVEETANSIMNNTVRVIIGHKEAANQQIEQKLTFCGNEEGKLIAIRQLIQSGELKPPVIIFLQSITRAKALYHELLYDKLNVDVIHAERTQIQRQKIIEGFKKGDIWVLICTDVLARGIDFKGVNLVINYDVPNSAQAYVHRIGRTGRGGREGKAITLYTKEDSIAIKPIINVMKQSGSKYDEWMDNMTKISKKQKQSIKNGQIKRNDISTVPKVVKEKRKRRQEMIQASKRRKQGKLEDKDSGSESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5W8</accession>
    <name>A0A1E3P5W8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_78847</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039444.1">
        <property type="nucleotide sequence ID" value="XM_019184796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5W8"/>
    <dbReference type="STRING" id="683960.A0A1E3P5W8"/>
    <dbReference type="GeneID" id="30202042"/>
    <dbReference type="OrthoDB" id="9439903at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051643">
        <property type="entry name" value="Transcr_Reg_ZincFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24396:SF19">
        <property type="entry name" value="FI01119P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24396">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="281" mass="32166" checksum="4464E5E6A74181B1" modified="2017-01-18" version="1">MQNLIDDPVFYNLLVNNRESIGSTSSNATNSPQEKQQQQQPNAVEQFIKNEQVDFSFLNNVEFPLMNNNNNNNSISTPSETSYQASIFSENLSLPNQNYDTDALDDLLSKDINFQISSELPPKPTPSTTKQFHKFYTSTINSQSGQLQTSTSSIAKPQLTYHKCPFCQRQFKNKSYLSRHLKKHDTVKDFKCPFFNEQHTKCHHLDGEFSRKDTFKAHLKSIHFIYPIGVAKIDRNSSMGRCAGCFQEFKSNNDWLNEHIEKKSCPGFAINQKSDENASKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P680</accession>
    <name>A0A1E3P680_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">fumarate reductase (NADH)</fullName>
            <ecNumber evidence="7">1.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">NADH-dependent fumarate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_77545</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>succinate + NAD(+) = fumarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18281"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.3.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.6" evidence="7"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040080.1">
        <property type="nucleotide sequence ID" value="XM_019184570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P680"/>
    <dbReference type="STRING" id="683960.A0A1E3P680"/>
    <dbReference type="GeneID" id="30201816"/>
    <dbReference type="OrthoDB" id="10254877at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016156">
        <property type="term" value="F:fumarate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000007">
        <property type="entry name" value="NADH-dependent fumarate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050315">
        <property type="entry name" value="FAD-oxidoreductase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43400:SF7">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE 2 FAD BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43400">
        <property type="entry name" value="FUMARATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="9">
        <location>
            <begin position="6"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00077246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ60873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="249" mass="27237" checksum="C236926CE6740B62" modified="2017-01-18" version="1">MLLGLPTTNGAQTLGEGQVLLEKIGAELIDMEQIQIHPTGFINPKDPGSRWKFLAAESLRGIGGVLLNTDKNERFINELSTRDVVSQAILKQQDSKALLVLNDDMYQDFKFQLDFYIKQGLVVKTSVKDYFKENAGKVVDLLSRYSKESISDEFNREYKAHVFKEMKVSSELLIAEITPVVHFTMGGVKINGDGQVLDTKGDVIEGLYAVGEVSGGVHGANRLGGNSLLECVVFGTSAAKRIAGELGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6E7</accession>
    <name>A0A1E3P6E7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Superkiller protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_27706</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040041.1">
        <property type="nucleotide sequence ID" value="XM_019181402.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P6E7"/>
    <dbReference type="GeneID" id="30198648"/>
    <dbReference type="OrthoDB" id="421075at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055087">
        <property type="term" value="C:Ski complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070478">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039226">
        <property type="entry name" value="Ski3/TTC37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040962">
        <property type="entry name" value="TPR_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15704">
        <property type="entry name" value="SUPERKILLER 3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15704:SF7">
        <property type="entry name" value="SUPERKILLER COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18833">
        <property type="entry name" value="TPR_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="38"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="638"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="672"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="939"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1140"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1342" mass="152693" checksum="E83BFB977344264A" modified="2017-01-18" version="1">MSEAKGYLKAAKQALKDDNPEYCIQLAEDALKIDPKNYFAFVFIGKSSHLLQQIDPAIKAYKKAIEVDHDNITAWKGLFLIVSNHNDYKQFFEFAGDYAELLVSLQEGLSEFIREIRKYLKNNSSDEVYEEYLRSLLPENKLGQVCLGIIEKPQKVITEYLDIQLKRENSEVSKKTTRERLKSKNQQLMDKIAWEYYKDSVIPHLYEELINTLDDDEKRRETEEQYLKYRYKVLKAAPSTAKPDLFASIKEMIEGMVLVKHTSFFAWSLYFDWIDSSDLSTIDETTLKNFLKLFKSEPLGQIIYAYVLSDLSPFKIDIEEKKKPSKSKELSQEEQQLEQLETDETDSALAPEELLALMVQGLESCKTSLLAYRIVLSYQIHLKLFESALATCQAAIQLLVTSTRHTGFDFKNSKEDLLVNYGVVLTYYEAPKNFKRALQIFDKILVESPTNVNARVGKGLILVERGELQDARTLLAQVTEEFPDNDEAIMELSWCQVKLGEYQTGREGLCQVLSNLKGNTLHILELKASVNWKIAQSYLLEGGNVDKAYEYLVESLRASKNFAPSYTSLGEIYLNHYEDEKRSAKCFLKAFEIDSNEVTAAWYLVKDFTAKTDWPRAEMFCKRLIISENSKRRLGNDSWPYRILGCASLETQDDAKAVEWFQTAIRLNANDTESWIGLGEAYYGCGRLEAAAKVFRRALESEPEHWVAQYLLAVVETHISEFDVAIEIFGKLLGERPNEECVVAGLYDALYQYANECVSRGFFGKAIGLVLKAVELLPKGNKQSLNFWKITGDLVHIFLIVQSKIDQFPHKEFLELFQSVDVDLDSSLIDEYIEGEKLVELVALFLVYCNQAALSLNPSSRPLRSSSFYNLGLSQLIAYLKTKNTEFRDSSIISLKESIKLQSNYAEPWIALGVASISVNPRVAQHCFIKGSSIDPKNVEIWSDLALLYLRYDDPELAVESYMRGQSLAPSEPISWLGHALAAQAQGDFELASNLFTHAFILSNGRSPVAQLLYGLNICLKRIGKGDDVQDVEAVQELSTACYGMLQYLKHYPLDPFGLSVSCLILEKLNDYELGQEISTQLLSILEKAYEESESDDVLLNFARIKAQSARFELGVQNYEGAIECAQECLELTEDEKTVISSRTVLGLSYFFLDKFDEALEEFKLILSLSNDAKRLVVLISQVLYIYDTEETKQAALDQLFHNIETAGTSLLVTLVIGAISVVENLEDYLVIIKSELEALPLSELISDKFKNVPYLVSQVNRRLKKNEQFTWQRSAYFFPDDLRIWENLDRNISLKIATHGKVNAKDLSDAYLNTGELRDIQRSMFLSPWNVASAEALKGCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6I0</accession>
    <name>A0A1E3P6I0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CTLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_61595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040238.1">
        <property type="nucleotide sequence ID" value="XM_019183207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6I0"/>
    <dbReference type="STRING" id="683960.A0A1E3P6I0"/>
    <dbReference type="GeneID" id="30200453"/>
    <dbReference type="OrthoDB" id="3980019at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTLH" evidence="1">
        <location>
            <begin position="86"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="252" mass="29253" checksum="55F8AAF8A4E04E55" modified="2017-01-18" version="1" fragment="single">MSNTSNISGNNNNELDWDALMESNLIDKQSLNHLVLNYLIFEGYESSTVKFAKEIGIDLNNKGTKQDDDEDEVNPLQKNLLMGINSIKIRNEIKLNILNGEVQLAINKLNLNYPDLLEQNNFLHFKLLLLNLIEMIRNNNDDSKNDDNNQELFVMNIIKFAQDKLTNKAIKNVKFMEELELTMTLLLYSNNKDLLPAKLNELFEFKLRRDIADLVNKSILVKDSNNLIDLNYKKKFYEVKHEDNDNNNGNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6Q0</accession>
    <name>A0A1E3P6Q0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor CWC15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_61199</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039839.1">
        <property type="nucleotide sequence ID" value="XM_019183126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6Q0"/>
    <dbReference type="STRING" id="683960.A0A1E3P6Q0"/>
    <dbReference type="GeneID" id="30200372"/>
    <dbReference type="OrthoDB" id="30179at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006973">
        <property type="entry name" value="Cwf_Cwc_15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12718">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12718:SF2">
        <property type="entry name" value="SPLICEOSOME-ASSOCIATED PROTEIN CWC15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04889">
        <property type="entry name" value="Cwf_Cwc_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="63"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="91"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="222" mass="26248" checksum="4E46CD783DF59837" modified="2017-01-18" version="1">MTTSHRPTLESRKGRDLQQTSIIHSRQVAAHKQLKYRKTTTIDPVDAKEKFNELKLELLQREKTHFKDSKGDDYDYKDRQLAIEDGEVESYEDRMERVAKETKDLDDSSDEEEDQEDEGSESESDEDDESDDDAELLKELEKIRKERAQAELQKQLKEKEESAINSNPLLSNNNSTGVRKNWRQDKVFNYKNNQEKHKTDDDYVNDLLRSDFHQKFLNKYVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6R4</accession>
    <name>A0A1E3P6R4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_90341</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040323.1">
        <property type="nucleotide sequence ID" value="XM_019186231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6R4"/>
    <dbReference type="STRING" id="683960.A0A1E3P6R4"/>
    <dbReference type="GeneID" id="30203477"/>
    <dbReference type="OrthoDB" id="1936594at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044244">
        <property type="entry name" value="TTC27/Emw1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="640" mass="72629" checksum="E06ADC74D97F9EF8" modified="2017-01-18" version="1">MEHLALPNLLSARKLTNFEFVLTGAKAKRTKFQQVAHSGLIILAKSNYFNAIKSSGKNQTPENFDLNSDLLLEKPHFEDIGNENLDEQIIKKQKTDLQEIDYSTLLPVSLRSEYIPESLKALDPNNQPTLSDYDNIQLLLRLYTLRQTSPSNDALISSQLMSLVARVLYQSQNTSVNYTVFSRALWERSILETTKARTIERGILQMQSLVEEIGLKIQTKYIPSTTEVSSAATRSKFLFQLPLIPRWDLDTKLAEKFMSLGLVKSAIEIYERLGLEADVALCHASIGEESKALEIIEQRIQTHPNDARAISIKGDLTGNPEYWYKAWDMAKYVKAKVSLCKYYYYPPQGVTKNIEESIKHMRDALSINPLNFANWYFYGCLGLESSQFELAAEAFTRCVALDDSNSLAWSNLASAHVSLDRTKEAFAALKKAVATDDNKKSWRIWDNYLLVAAKLRDWDEVLLACKRLVESKKEKSGEGSIDIPIVEKLVEILVSNPYPEDECELTFFQKSCLEFTTVTLPSVITSSARLWRIIARVELWRKKPWASLDCHEKAYRAISHNPDLEVDEKVWNETVEACSDLVAAYESLGELPGKHGAGDLVCKDWKYKAKSTIKSLMSKGKQSWEDSDGWEQLTELRSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6S7</accession>
    <name>A0A1E3P6S7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Beta-lactamase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_83354</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040336.1">
        <property type="nucleotide sequence ID" value="XM_019185527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6S7"/>
    <dbReference type="STRING" id="683960.A0A1E3P6S7"/>
    <dbReference type="GeneID" id="30202773"/>
    <dbReference type="OrthoDB" id="428260at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050789">
        <property type="entry name" value="Diverse_Enzym_Activities"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43283:SF3">
        <property type="entry name" value="BETA-LACTAMASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G07500)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43283">
        <property type="entry name" value="BETA-LACTAMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase-related" evidence="1">
        <location>
            <begin position="22"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="420" mass="46974" checksum="8BE4C0F573666F8C" modified="2017-01-18" version="1">MPVPNTPPILSEKAIESLKKVIDQSVQNPKKDIPGVSVAITNSKGDDLFSYAKGVTGADNTDEITTDSIFWIASCTKLLASIALMQLVEQGKVDLDSSEQIEKYIPELTRLPIVSHDNEDGLNLKPRKNKITLRHLLSHTSGLGYSFFSDILKKYANFFHVDEFDVSSERELLLPLLFEPGTNWSYGVGIDWAGVLLQRVTGQTLNDYIVEHILDPLGVEETGMEPEETLRKKFVQMNVRDKNGELSTTDHIYKKVLKGDYQTLFHSGGAGVFSKPKEYIKVLATILNDGVSPKTGKQILKKETIDLMFSNQIEKFPNFGRAGIKSSDDFLTNSIADIYPQGDLPQGWGLSMFLNLHETSTGRGNNSGWWCGLANLFWWIDREHDIAGFVGAQILPFADIKVMTLWAQVEQEIYKDLGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P739</accession>
    <name>A0A1E3P739_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Holocytochrome c-type synthase</fullName>
            <ecNumber evidence="10">4.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_28048</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Lyase that catalyzes the covalent linking of the heme group to the cytochrome C apoprotein to produce the mature functional cytochrome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>holo-[cytochrome c] = apo-[cytochrome c] + heme b</text>
            <dbReference type="Rhea" id="RHEA:22648"/>
            <dbReference type="Rhea" id="RHEA-COMP:10725"/>
            <dbReference type="Rhea" id="RHEA-COMP:10726"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:83739"/>
            <dbReference type="EC" id="4.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cytochrome c-type heme lyase family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.17" evidence="10"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039979.1">
        <property type="nucleotide sequence ID" value="XM_019181443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P739"/>
    <dbReference type="STRING" id="683960.A0A1E3P739"/>
    <dbReference type="GeneID" id="30198689"/>
    <dbReference type="OrthoDB" id="1158011at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004408">
        <property type="term" value="F:holocytochrome-c synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000511">
        <property type="entry name" value="Holocyt_c/c1_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12743">
        <property type="entry name" value="CYTOCHROME C1 HEME LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12743:SF3">
        <property type="entry name" value="HOLOCYTOCHROME-C SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01265">
        <property type="entry name" value="Cyto_heme_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00821">
        <property type="entry name" value="CYTO_HEME_LYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00822">
        <property type="entry name" value="CYTO_HEME_LYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0349">Heme</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="263" mass="30235" checksum="2D6CC1DB55D3CBB9" modified="2017-01-18" version="1">MGWFWADNSENVKQASSDVSAAKCPVNHGNITEVPAVCPMKKDNGQTDDTLNPLNNMPSFISSSKLPGQKLDLPTERTFSTIPRGEDENEGVWEYPSPQQMFNAMMKKGKSDGIPEDAVESMVDVHNFLNEGAWQEILEWEKPYVEEYKKQPRLLQFTGRPHDLSPRARMYLQLSKVFPETFNTEPPFDRHDWYVLRANGSGSWDQVRYVIDYYGGPDDPHGMPTFFLDVRPALDNPTNAKDRMRHWLKPYWDKAMGKESNDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P753</accession>
    <name>A0A1E3P753_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SDE2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_67852</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SDE2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040463.1">
        <property type="nucleotide sequence ID" value="XM_019184102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P753"/>
    <dbReference type="STRING" id="683960.A0A1E3P753"/>
    <dbReference type="GeneID" id="30201348"/>
    <dbReference type="OrthoDB" id="547031at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051421">
        <property type="entry name" value="RNA_Proc_DNA_Dmg_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053822">
        <property type="entry name" value="SDE2-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786">
        <property type="entry name" value="SPLICING FACTOR SF3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786:SF1">
        <property type="entry name" value="SPLICING REGULATOR SDE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22782">
        <property type="entry name" value="SDE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SDE2-like" evidence="10">
        <location>
            <begin position="95"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="201"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="208"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="223"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ61256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="278" mass="31379" checksum="C563BD88B2C6A3A9" modified="2017-01-18" version="1">MSGSEEKCIIFVKCINGIDDFQFDVPMDSSITNVKQQVRDRLPPSVVPRASISSMGGLDINPYKNVHDLYYSVTSNQASDFTKFLNLKINICLCGGKGGFGQLLKAQAHSMSKKKNRKNLKSNSKDYFKTLDGRRVKTIKKIKQLDEYMSTLDEHEKTKIAEKKAKLQKILDVDLNKNVKFEDTKFLDDVEKQLEEIRESVNYVESSSSDDEISSDDESDEGNSQSIQEGSSASSSSSTKNRRSKFTSFFDEDEDDDEIEKEQSTKSLPNSKGKEIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7N3</accession>
    <name>A0A1E3P7N3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">6-O-methylguanine-DNA methyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">DNA repair MTase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">O-6-methylguanine-DNA-alkyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_29485</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040610.1">
        <property type="nucleotide sequence ID" value="XM_019181613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7N3"/>
    <dbReference type="STRING" id="683960.A0A1E3P7N3"/>
    <dbReference type="GeneID" id="30198859"/>
    <dbReference type="OrthoDB" id="2548197at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032132">
        <property type="term" value="F:O6-alkylguanine-DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070911">
        <property type="term" value="P:global genome nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052520">
        <property type="entry name" value="ATL_DNA_repair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42942">
        <property type="entry name" value="6-O-METHYLGUANINE DNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42942:SF1">
        <property type="entry name" value="ALKYLTRANSFERASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="Methylated DNA-protein cysteine methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding" evidence="5">
        <location>
            <begin position="10"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00030795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00031621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00033095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="125" mass="14329" checksum="2684013ED7AD59D6" modified="2017-01-18" version="1">MSRQDEARAFHYAVYEAVQLIPQGRITTYGHIAKLIYKPRNSRQVGQALKFLPREGQEHEYPYSTANVPWWRVVSSAGVISPREGGAVQLQADLLRQEGVEVDGNNKLKLSEFGWFPETEFDEYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7S4</accession>
    <name>A0A1E3P7S4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ribosomal RNA small subunit methyltransferase NEP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_28725</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040676.1">
        <property type="nucleotide sequence ID" value="XM_019181533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7S4"/>
    <dbReference type="STRING" id="683960.A0A1E3P7S4"/>
    <dbReference type="GeneID" id="30198779"/>
    <dbReference type="OrthoDB" id="269804at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005880">
        <property type="term" value="C:nuclear microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070037">
        <property type="term" value="F:rRNA (pseudouridine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18088">
        <property type="entry name" value="Nep1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000003">
        <property type="entry name" value="Ribosomal RNA small subunit methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005304">
        <property type="entry name" value="Rbsml_bgen_MeTrfase_EMG1/NEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636">
        <property type="entry name" value="NEP1/MRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636:SF5">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03587">
        <property type="entry name" value="EMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ61469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="245" mass="27209" checksum="991517B07146C083" modified="2017-01-18" version="1">MTESKKKEVLPASLVPEQPTPLNSKDKDTKRLIVVLSAACLETHKISSGHGSGGQDKYALLNCDDHQGLLKKMGRDISEARPDITHQCLLTLLDSPINKAGKLQVYIQTARGVLIEVNPSVRIPRTFKRFSGLMVQLLHKLSIRSVNSEEKLLKVIKNPITDHLPIKCKKITLSFDAKLTRVQDYVEKLDQDESICVFVGAMARGKDNFADEFVDEKIGLSDYPLSASVACSKFCHGCEDAWGIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P857</accession>
    <name>A0A1E3P857_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Galactose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="4 15">2.7.7.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_25040</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>alpha-D-galactose 1-phosphate + UDP-alpha-D-glucose = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:13989"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.7.7.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.12" evidence="4 15"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040765.1">
        <property type="nucleotide sequence ID" value="XM_019181090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P857"/>
    <dbReference type="STRING" id="683960.A0A1E3P857"/>
    <dbReference type="GeneID" id="30198336"/>
    <dbReference type="OrthoDB" id="418412at2759"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008108">
        <property type="term" value="F:UDP-glucose:hexose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
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            <begin position="27"/>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
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                <person name="Salamov A.A."/>
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                <person name="Barry K.W."/>
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                <person name="Deshpande S."/>
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                <person name="Meier-Kolthoff J.P."/>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="126" mass="14391" checksum="7173D03A5D8F1D9D" modified="2017-01-18" version="1">MNTQPGDYKYPEQQQQQPQQQQQQQSQQPVVIVQHPEQPEQPRQYSTLSKVLLYISVIIPPIPVIIVATDANDIFINLCLLFLFGVGALFHSIYVVYKYTSGEKSYGAWIKKYGHKNEKRPSGGNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8I3</accession>
    <name>A0A1E3P8I3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_75919</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040926.1">
        <property type="nucleotide sequence ID" value="XM_019184293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8I3"/>
    <dbReference type="STRING" id="683960.A0A1E3P8I3"/>
    <dbReference type="GeneID" id="30201539"/>
    <dbReference type="OrthoDB" id="10267824at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000020">
        <property type="entry name" value="60S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.950.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000006">
        <property type="entry name" value="60S ribosomal protein L8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="entry name" value="Ribosomal protein L2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023672">
        <property type="entry name" value="Ribosomal_uL2_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_uL2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_uL2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007180">
        <property type="entry name" value="PRK09612.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL2 RNA-binding" evidence="5">
        <location>
            <begin position="11"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL2 C-terminal" evidence="4">
        <location>
            <begin position="96"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="254" mass="27347" checksum="CB6992BE30BC6676" modified="2017-01-18" version="1">MGRVIRNQRKGAGSIFTAHTRLREGAAKLRTLDYAERHGYIRGVVKQIIHDAGRGAPLAKVVFRDPYKYKLREETFIANEGVYTGQFIYAGKKASLNVGNVLPLGSVPEGTIVSNVEEKPGDRGAVARTSGNYVIVIGHNPDENKTRIKLPSGAKKIVSSDARGVIGVIAGGGRVDKPLLKAGRAFHKYRVKRNSWPKTRGVAMNPVDHPHGGGNHQHIGKASTISRGAVPGQKAGLIAARRTGLLRGSQKTQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8J2</accession>
    <name>A0A1E3P8J2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_26698</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMC family. SMC2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040928.1">
        <property type="nucleotide sequence ID" value="XM_019181293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8J2"/>
    <dbReference type="STRING" id="683960.A0A1E3P8J2"/>
    <dbReference type="GeneID" id="30198539"/>
    <dbReference type="OrthoDB" id="10255539at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034506">
        <property type="term" value="C:chromosome, centromeric core domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000791">
        <property type="term" value="C:euchromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990814">
        <property type="term" value="F:DNA/DNA annealing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003680">
        <property type="term" value="F:minor groove of adenine-thymine-rich DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903342">
        <property type="term" value="P:negative regulation of meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070550">
        <property type="term" value="P:rDNA chromatin condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070058">
        <property type="term" value="P:tRNA gene clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03273">
        <property type="entry name" value="ABC_SMC2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1060.20:FF:000005">
        <property type="entry name" value="Structural maintenance of chromosomes 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000278">
        <property type="entry name" value="Structural maintenance of chromosomes 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000385">
        <property type="entry name" value="Structural maintenance of chromosomes 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027120">
        <property type="entry name" value="Smc2_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="831"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="253"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="682"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="746"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="960"/>
            <end position="1009"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ61721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1169" mass="131987" checksum="1F0A0CD2077CB5D9" modified="2017-01-18" version="1">MRVEELIIDGFKSYATRTVISGWDAQFNAITGLNGSGKSNILDAICFVLGISSMSTVRASNLQDLIYKRGQAGVTKASVTIVFDNNDTAKSPIGFESVPKISVTRQIVLGGTSKYLINGHRAQQQAVLQLFQSVQLNINNPNFLIMQGKITKVLNMKPAEILSLIEEAAGTKMFEDRKDKAEKTMAKKETKLQEIRSLLAEEIEPKLEKLRNEKRMFLEFQQTQSDLEKLSRVVNAHDFTKYSKRFYKLKEEYESKQSIMENSSSEIERLTNEIATLTEDLEKFKERKAQEAKKGNKLKDLEARQNELSKTITRLNTSKGIISENMKDEEKKKQKLINQIQTLELDIEKRLNAHKNLEQEYNQAKSRLSELKETHTKKEELLSTLTTGVSSKGKSGGYNAQLQEEKQKLAAANVSIQQSKMKVEHLVSDNKSNQPKLTKAKKEHEAFLQEINQLKQKQADLEGQLTKVGFDPSKVKDLKRREVEINRELHKVTNEVNYLKRKVANLEFSYTKPTSNFNARSVKGVVAQLFSLDEDKAASATALEVAAGGRLYNIVVDNEVTGSQLLERGQLRKRVTIIPLNKIAARTLSQQQVHIAKELCPGKVELALNLIGYEAEVSRAMEFIFGTRLLCEDADTAKKVTFHPQVRARSVTLQGDVYDPEGTLSGGSRKNNGSVLVNIQNYNKVNNHLKSLQNDLKNFQFELSNEQELSDKTKSIQNELSLTSHQIQLAEKNLRTNASSQILYKFENNEKEIQELTEAVQEKQGLVQSINNEIAKIENDMKEFSSNRGSKLQELEKEVGSLAAEASKLEQQLNNAEDTFQSSQIELEQLGGELQTAKESVGDVDATIEDLKTQAVSVEKELQDQGKLIDEINAQIDVERENQVGINEEIAELNKVLKSKNQQVNDIKLNSQKLTHEVEKLAGSYKSLGKHLEELVEENSWLNDENIVNSVIQQYPNINLDECHEQIARLNERFQGMKRKVNSNIMSMIDNVEKKETALKQMIKTIEKDKSKIEETIVTLNEYKRDTLVKTWEKVSTDFGQIFGDLLPSSFSKLVPPEGKDVTEGLEVKVRLGKVWKESLVELSGGQRSLIALSLILALLQFKPAPMYILDEVDAALDLSHTQNIGHLIKTRFKGSQFIIVSLKEGMFTNANRVFRTRFQDGTSVVSVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8L0</accession>
    <name>A0A1E3P8L0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carbohydrate-binding module family 21 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_83408</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040414.1">
        <property type="nucleotide sequence ID" value="XM_019185538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8L0"/>
    <dbReference type="STRING" id="683960.A0A1E3P8L0"/>
    <dbReference type="GeneID" id="30202784"/>
    <dbReference type="OrthoDB" id="3981307at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001069">
        <property type="term" value="F:glycogen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005979">
        <property type="term" value="P:regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2440">
        <property type="entry name" value="Carbohydrate binding type-21 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005036">
        <property type="entry name" value="CBM21_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038175">
        <property type="entry name" value="CBM21_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050782">
        <property type="entry name" value="PP1_regulatory_subunit_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307:SF51">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT GAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03370">
        <property type="entry name" value="CBM_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51159">
        <property type="entry name" value="CBM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM21" evidence="2">
        <location>
            <begin position="293"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="505"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="579" mass="65656" checksum="DF2E7E0A3E937282" modified="2017-01-18" version="1">MERSRETAGSVPNERSSGYNALTREKLFLLNLENNNMKNMKQHQEQRLSIINNPYNSYTTTPELSNSSSSSSLNSSESDPAITGHDHFYKFYNNGSDSSIATVVQKKLSSTHSNSTLNLQNLDSHQEPVEKPQLEHDEMIVDVPVITASSTATTTDDDLSTTSEDQMTIPRSLKSSLKLPSLKRSKSLPSTKTVRFSTDLTKVKTFNEYAKPSSISLENSPDQSPPYYSNTNGRAYFDLKEDLFTNQSTPWFDQSSSSSEEEDDEEDNYFYKTPSSISQNKWKLKSMNFDLFRQSDPQSYVKLVSLNISKDSIVGLVQVLNISFEKQVEVKFTVDCWNSIYIVNASFDRSLNSSRDQFRFEINLNNSLLINSKRSKNQPINIELCVKYSSSNQAVFYDNNNGSNFKFQMVSNKQHRYSSNVKRSSQRPTYSTLPSVSSTAYDDNFASAILSSPDYYSSNENYTTSRYFPDNTDYFNTKFQGSNFQSTNNHSSIPSWSKNYASTSSSNTSSTVSSISSNISTTSNSSNETIKASNNNNESLNNLNAQSLEKSRQHYNEFLKKYCFFKSDDKKDHESIFIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8M4</accession>
    <name>A0A1E3P8M4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_24864</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040867.1">
        <property type="nucleotide sequence ID" value="XM_019181066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8M4"/>
    <dbReference type="STRING" id="683960.A0A1E3P8M4"/>
    <dbReference type="GeneID" id="30198312"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000065">
        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF15">
        <property type="entry name" value="TRANSPORTER SEO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="331"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="375"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="424"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="458"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="492"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="575" mass="66248" checksum="0CE7031DA15F37B3" modified="2017-01-18" version="1">MISKDYSAWYWKFIPHERKVEDLSDDEKSFDVTGSEFNVEEELEFRDEANRKWWKFFDEYEYRYNKQTRSQKSWWRFFDKNASSAEKKLIIKIDFLIMTFAILSYWSKNLDQANINNAYVSGMKEDLNMQGNDFSNAQALFQAGTVIFQIIFMYLFPRVPIHFLFFAGDLMWSAITFATAFIHTPQQLHVCRFLVGAGESGYFIGMHYLLGSWYKADELGRRGGLFYCGQMLGTLTAGLVQAAISQNLDGVHGRAGWRWMFLVDGLATSSVAFIALYLVPGTPENCCSLFLTDDDIRLARYRLKKANIAPPSKDPPPFFNKKLWKKIIFDWRIYILGVLDYLFWNAVNTAFSNFALWLKYLDRYDTPHLNRLTSIPPALGILWILLVCGSADILKSRAFAIFWAEVFVLMGNIVLAKWDIAEPAMWYAFMISYFGLSVSSVVYAWLNDIMRYDSQERSIVLCAVNIFGNQSTAWTLPTVFRTSTAPRYHQGYVFVAGHSAALMVWSYVTLFFYKRQERKDARSNGIVLYNSKTGDISVEARKHLETESAAGGVFEADSNSLTKTQELLEVRSISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8S5</accession>
    <name>A0A1E3P8S5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_76000</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041016.1">
        <property type="nucleotide sequence ID" value="XM_019184307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8S5"/>
    <dbReference type="STRING" id="683960.A0A1E3P8S5"/>
    <dbReference type="GeneID" id="30201553"/>
    <dbReference type="OrthoDB" id="3979953at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="280" mass="32432" checksum="07171090406B6142" modified="2017-01-18" version="1">MVKPATQRRRNKVYQQQLQAQQAQQAQQAAKQKEDEIGGMHDEADVMSFRSAATHRFLLNQELIENVTEKWVHTDAIVKPKPFAHFQTTKENIDEIKDELYFGNVKLMRQKLEKLEKELEEVKKEQESIDEKTNNKIIREVVDQLSNAFVNRDSSNFENFEKTVQEKINKPLVDHLYEIKSFPQINNDTAQAPPNYWEELAKAKEEQAAQAAASLNNHLVMTDHSYMQEPVQNVQEQLQYVEAPPAFGNDDLNMMDDVGFGLHSTLDDQDFLSQIDHSME</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8T4</accession>
    <name>A0A1E3P8T4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_76016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041033.1">
        <property type="nucleotide sequence ID" value="XM_019184310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8T4"/>
    <dbReference type="STRING" id="683960.A0A1E3P8T4"/>
    <dbReference type="GeneID" id="30201556"/>
    <dbReference type="OrthoDB" id="294251at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000026">
        <property type="entry name" value="TBC (Tre-2/Bub2/Cdc16) domain family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="422"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="670" mass="76444" checksum="0E3B46DEDEE961C4" modified="2017-01-18" version="1">MSNNSRGQRMSISRSRSQTSTKKNSIPENLGPLYDDNGPEGGSSEFLASLNAPYHAKMSSESVDKIGRSSSPSRGLFETHQLNPESIPGNLHVSPNKSIEHLIKRYGAVTLIRQLSTDLAQREAEMSLIRKRNSTRENTLMKLLNEMGLSTSQIELILRERLDKSEMSLKDINDNSYLKDLINDAMSEHFDDVLQPVDSRTSIATSPATLRTPQRSSTSVTLNAGKQSSTQKVSTRPHSMSSPPLPSNDLKQHHNNSIISQQHGSNSILNVVSHRFLNHVPQPVHDAIVNSSTSNNKRLVPMEMENFDPHDVKPPTYQEQSTEDDRGYIDKFGFMYDRENKDGTKITSPRNPIDKSFTSRLLEIANDYDKTQHQNNKQWDAFIKKISLLNNENSDDLLVINGENLSNFKHLYKDFTKLVQNGVPMKYRTKIWSELTGSKNLMTPSEYYKLVYETKANEEAESQIELDLYRTMPFNIFFKDNGPGLKKLKNILIAFSRKYPNIGYCQGMNFIAANLLLVYPNEEEAFWAFIGLVDNVLPKDFFNLVNVKNDLTIFKENFIENLPKLNAHFNEIDVEIEPICFNWFISLFSDSLPIHIVFRVWDIMMLNGYTEIFKISIALFKTFEKNLLGFDSNVDVYEFMKNLNTANFNLKGTELIKISSSIILNTSEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8Y6</accession>
    <name>A0A1E3P8Y6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dipeptidyl-peptidase IV</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_25349</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S9B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040977.1">
        <property type="nucleotide sequence ID" value="XM_019181134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8Y6"/>
    <dbReference type="STRING" id="683960.A0A1E3P8Y6"/>
    <dbReference type="GeneID" id="30198380"/>
    <dbReference type="OrthoDB" id="16520at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF160">
        <property type="entry name" value="DIPEPTIDYL AMINOPEPTIDASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="8">
        <location>
            <begin position="84"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="7">
        <location>
            <begin position="542"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="749" mass="85729" checksum="74D1A77489E390C2" modified="2017-01-18" version="1" fragment="single">LEKITMESLRSGKFFVYDSELSFINPSSGLAEFDDQGLYYTRSHDKIVAEKGTDKSYSQVLLPNFEFQHEGISHKVVKYYPTYDLTNAIVATEMETVYRHSSLAYYWIHNIAENSFTPLATGEQGALVKLSFATWSPKYNYISYIQDNNLYVKSLKTSDIKQITNDGSKTVLNGRTGWVYEEEVLATGKALWWSSNEKHLIFMRTDESGVPSYGIDYYVQDPSGAYKYPKTKEIPYPKPGFSNPKVSMKVYSVESDETHEIKRESDLGDEYIIYEALFIDDANFIIKETDRESNILHVRHYNPVSDESKVVYKVDAVKEYNGWIEKFNSPIPIPPKSGSSSWGYVDTIVVNGYNHLVYFESTTTETPKALTSGEWEVLNAPLAYNKEKNLVYFAANKRASTDKHIYSVDLETGKIDSFTDDSDQAYYEAEFSPNAKYVSLYYKGPETPVQKLFKVEGKLEMKLLSISTYLEMSKRTHAFPKKNFHKVEVKTAEDETPVELNVIEYLPPNFDSSKKYPLLVHFYGGPGSQVVNSKFDVWFEDSVSSALDAVVLYVEPRGTGGKGWKFRSWQRKKIGYWEPRDITEVAKTWISKGFIDADRTAAWGWSYGGFTTLKTLEFDAGETFKYGMAVAPVTNWLFYDSIYTERYMDKPQNNKEGYEISQISKVGNLGKANRFLIMHGTADDNVHIQNTYSLLDQLDLGGVENYDVHVFPDSDHSIYHHNANIIVYDKLFSWLRDAFQGDFDKLGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P944</accession>
    <name>A0A1E3P944_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_78028</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040604.1">
        <property type="nucleotide sequence ID" value="XM_019184649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P944"/>
    <dbReference type="GeneID" id="30201895"/>
    <dbReference type="OrthoDB" id="3980992at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="86"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="126" mass="13934" checksum="4999F26CA5B31C92" modified="2017-01-18" version="1">MSSKEPTPINTAAASASASTDSFVDAPESADAIETVEVDDKDTLKEEPLATPNHSLIQSNGSEENQPEESEKKTDVVTIFDVATEIENMLKEIDSKIDENDQLVQQKIEKINKTLDKIENKMRNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P957</accession>
    <name>A0A1E3P957_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_59983</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041115.1">
        <property type="nucleotide sequence ID" value="XM_019182911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P957"/>
    <dbReference type="STRING" id="683960.A0A1E3P957"/>
    <dbReference type="GeneID" id="30200157"/>
    <dbReference type="OrthoDB" id="508119at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000313">
        <property type="entry name" value="MFS quinate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF8">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="207"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="366"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="472"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="34"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="559"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="559"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ61908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="594" mass="66227" checksum="BECC3694AE60752D" modified="2017-01-18" version="1">MTAAVVHPKQSLRKKFFNKDNSPPEIFNRTLYISVFIFGILGCARGLDEGGISGSVAQKSFKHQFGLDDESKTEHYLANLKSNITSMVQLGSIGGCLMALYFVDKFGRIRTLQGVCVFWIASVIIQITAKDVGQLYAGRLIEGLSIGQTVVLGPTYLSEIAPKAIRGLCNCVFAGAVYFGIMMSYFSHYGTALHVSASSRVQWVAPTSLKIIFAGILFIGSWFCKESPRWLMKKNRVDQAYRNLSKIRNLPEDHPYILSEINDIQEHMKHEKEVMQDTSVLGLFKEFITVPSVRYRLFLGLGIQILGQWSGANAITIYMPELTALVGYTGTKKLLMTAILGVVKFTSAYLTAFFLIDFLGRRKCLYIGLTVQCLSTLYFAVFITVVPKVSNDEFTMTSSEKHAGVCALAMLYLNGVGWTMGWNSIQYLINSEMFNLRHRAIGTALIMTFHFANQFGNTKAVPSMMIAMHHGGTFFFFFGVIIIGMFFCWFFVPEISGRSIEGMEELFELPWYLIGRKGSKLAPDHSAVTQVHFDETGHGNAMGGDIEVKQDDEFVENVNSSDNGLMKNAQTNNTTTDVSTENGSNIDLEKQQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9C1</accession>
    <name>A0A1E3P9C1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_15330</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040684.1">
        <property type="nucleotide sequence ID" value="XM_019180641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9C1"/>
    <dbReference type="STRING" id="683960.A0A1E3P9C1"/>
    <dbReference type="GeneID" id="30197887"/>
    <dbReference type="OrthoDB" id="10030083at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03390">
        <property type="entry name" value="PAP2_containing_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF35">
        <property type="entry name" value="RE23632P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="7">
        <location>
            <begin position="69"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="221" mass="25225" checksum="353006530A321504" modified="2017-01-18" version="1" fragment="single">PFLREFLINDPSIQHPFTKFEQVNDTTCILISSLIPLISITLVLLYQNNFQPQKILQSKQRLIKFQLSILGLILTLSITGTITVFLKNLIARPRPDFIDRCQPDPSKLTSKLLYTIDICTRPDKELILEGLRSTPSGHSSISFSGMTYLTLFLCSQWRVFSNRTRLHFLFCAALPIFIAVWIALSRTQDYRHHFGDVTMGGMIGVVVSWGCFRKIFPSVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9C2</accession>
    <name>A0A1E3P9C2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_76095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041117.1">
        <property type="nucleotide sequence ID" value="XM_019184322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9C2"/>
    <dbReference type="GeneID" id="30201568"/>
    <dbReference type="OrthoDB" id="10595778at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="203" mass="23895" checksum="E76839A454908487" modified="2017-01-18" version="1">MSTEGEFHIIKEAIVDGEPINSTRTEQYITASPDSNISSIQHHLFIKSTRPRYVETLQDVSTLHQFLKDQLSFDEESPILEYSTSRIRHYEPILGYENLSDIYDYSKRFGTDPAEILQRLDALYGAIKDWDLDQVGRIIDNERFNRYVELLQDVDEVRMEREFKERVIKRMIIEQISSIVRPIKNDRSIKEKEINLVILLNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9C3</accession>
    <name>A0A1E3P9C3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_76194</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041220.1">
        <property type="nucleotide sequence ID" value="XM_019184339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9C3"/>
    <dbReference type="STRING" id="683960.A0A1E3P9C3"/>
    <dbReference type="GeneID" id="30201585"/>
    <dbReference type="OrthoDB" id="1747771at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF90">
        <property type="entry name" value="CENTROMERE DNA-BINDING PROTEIN COMPLEX CBF3 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="48"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="803" mass="91310" checksum="06FC01D38AE3E2BB" modified="2017-01-18" version="1">MEASATTNTEHNSVSKDQDTIMEENSSKSSSPEETAVHIKRRPRRSYNCGPCKKQKIKCDTQTPCGACKRHNRTDQCLASPPNPPSPDHKKRIKKISLSLGEPVNIKPMNQHQIHNNGSPVGVLPSTASGNSPDFKLPSLQAYTSSFKINSGGFEAPRDNLIPLGQRMQQLSTDASPYIASKENSISQIPRPWDRSSTSPGYNSSNSLFKPIKSEHDEVYQLRSQISLLTSRLTSLETKLESLDQKKSMKLAIDDNVFEYLSTIPHQVSINDASENSKSINIKFLQPDKLDNWDDFVQVIPDNLALSNIAQFFKLYLNPPMNLIDAKTLDYCFDSQKLDKGKSLVTQEDWECLSLVAMVCSLTVLSYPQDSIESHLNIKRTNALYVSNTLFQISKNCLNIVKYRENPKLIHLQILLLCSNYLKVFNKKNLLITTNSEMVSIAYTLGLQSLIIPQNSLEVETSQKIWWIICLNDTLNSLKFQIPPLIRLENLPKAQIGGSHILKAFISAEVSSFNSIITYIAKITKICSEIPLVSKQTTLEYVESLVIIDRQLTSLQIPHSFSLLNPRNDIVSNFQSCYLHFVLVLSRFEIYKKIYFTSQNSSIWLVMVSVLESFFKRYSELKNMYPPKDHLNNYLQISDHLIISAITNLLISISDPEILPRGYKQIIHSYFDSILEDLQLLRLSVLNDTTPFYNQSFKIIQRLSSVYQLQESRSLSAEVSAQIEESQKKQLEVSSKLKDSRVFESQESELGLVPSNEFFNVFLTSLYQEQRNSQQRSVTNETWSLKDVLDNDQIFFLRCIGIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA80</accession>
    <name>A0A1E3PA80_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_76488</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041520.1">
        <property type="nucleotide sequence ID" value="XM_019184394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA80"/>
    <dbReference type="GeneID" id="30201640"/>
    <dbReference type="OrthoDB" id="10549803at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="174" mass="20067" checksum="0F8D889CA05812FF" modified="2017-01-18" version="1">MSEQAPKNKSTPRRRYYNKNKNKKGAQKDNNLSSNSSSSASLSSLQSNKSRSAQRKPQLNPADSRDGEIRSLIYKLSILKTFKLINSNSKQKTFRIDINNDSYKFLIPIDKKHSTSIQSIEELSHNKFVIRNFNSKAKSNDKSLLFYVNYLINNYENLSMNPSDYQSFEVNKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAD3</accession>
    <name>A0A1E3PAD3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="7">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_82443</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="7"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041570.1">
        <property type="nucleotide sequence ID" value="XM_019185379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAD3"/>
    <dbReference type="STRING" id="683960.A0A1E3PAD3"/>
    <dbReference type="GeneID" id="30202625"/>
    <dbReference type="OrthoDB" id="407390at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901524">
        <property type="term" value="P:regulation of mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF25">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING)] KINASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Branched-chain alpha-ketoacid dehydrogenase kinase/Pyruvate dehydrogenase kinase N-terminal" evidence="8">
        <location>
            <begin position="58"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="457" mass="52436" checksum="7A6F52D28472D14C" modified="2017-01-18" version="1">MIRSRLTTTAPKTLKYINVIAKHLDDLGPYPPHSQYINPQHYYQNTVLMDWSNRTPHPVTLRQLAAFGRALNEEKIISSANFVRLELPIRIALKLRELQELPFNVVNNFHLARVYESYYDIFDRFRKIPQIRTIEDNEKFCKTLEDVLTDINLLNLPNLMMGALECCILNAMPQTQLDELVSSLLRARISRRLILEEHLSLTKNFTQNPNLPRSHNFIGDIFFKCSAKEHLQISANNAAKFISSLYPNVKSPKLIIEGQKDLSFQFLTSHLHYLFGEIMRNSYEATVKQFLLTNPTPTESDEPPPIRVCIIDNKDHVGFRFSDHGGGIDLKPIGRIFSFGKDSTNARESLANFHKLPNLELETLYKKPSNIKERQNSLMKTSIGGIDNNYIKETTLINLIQRPANYKLGLGLAMCKVYADYWNGDLVMHSLNGYGTDTYLKLGKLGHTQDKLQLDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAE0</accession>
    <name>A0A1E3PAE0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_27451</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peroxiredoxin family. Prx5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041596.1">
        <property type="nucleotide sequence ID" value="XM_019181374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAE0"/>
    <dbReference type="STRING" id="683960.A0A1E3PAE0"/>
    <dbReference type="GeneID" id="30198620"/>
    <dbReference type="OrthoDB" id="1882547at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03013">
        <property type="entry name" value="PRX5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000159">
        <property type="entry name" value="Peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037944">
        <property type="entry name" value="PRX5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430">
        <property type="entry name" value="PEROXIREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430:SF39">
        <property type="entry name" value="PEROXISOMAL MEMBRANE ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 7" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5 7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="32"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="6">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR637944-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="188" mass="20204" checksum="296F4AD9CFD0FEE6" modified="2017-01-18" version="1">MLPQIRSTLFRSAAPLRAQAVRGFRSCAPSLIQVGDSIPSVSTLREGSPGNEVDLAQLTKTGKSIIVGVPGAFSPACSASHVPGYVKHIKEFTDKGYKIFVTAVNDSFVTKAWGDELAKDSGIRFIADPSADFSKATELSIDATPFFGGVRNKRFVLIVEDGKVKSETVEPDNFGLDVTKAEEILKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAT8</accession>
    <name>A0A1E3PAT8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tryptophan synthase beta chain-like PALP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_82538</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041730.1">
        <property type="nucleotide sequence ID" value="XM_019185393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAT8"/>
    <dbReference type="STRING" id="683960.A0A1E3PAT8"/>
    <dbReference type="GeneID" id="30202639"/>
    <dbReference type="OrthoDB" id="10259545at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="1">
        <location>
            <begin position="21"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="356" mass="39060" checksum="9CF72355BC885057" modified="2017-01-18" version="1">MKQTTDSSEYPKITRDEFIKGVGNTPLIKIKSLSNETGCDIYCKAEFFNSGKSIKDRAAIYLLNDAMSKGLVQPGGTLVEATAGNTGIALALLGRTFNPPFKVRLYVPETLVQAKIDVLETLGAKVIKCPTNVPADHPESFNNKAINYCKENEEAFHVNQMDNLENKRSHYETTGPEIWRQTDGKINAFIAACGTGGTYAGIATSLKDVSKGEVKCYVADREFSGLHSFVQSKGTSWEHESGESFVEGVGRQSLTRNLDDILEFTDGSFNALDSEVILMLYKLLDEENLSVGGSSGLNICCAKKLAQTLPKGSVIVTTAADAGERYASKVFNKEWLKEKGHWDKIPEHLQKYATYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB03</accession>
    <name>A0A1E3PB03_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Luciferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_82575</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the NtaA/SnaA/DszA monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041778.1">
        <property type="nucleotide sequence ID" value="XM_019185402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB03"/>
    <dbReference type="STRING" id="683960.A0A1E3PB03"/>
    <dbReference type="GeneID" id="30202648"/>
    <dbReference type="OrthoDB" id="5561043at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051260">
        <property type="entry name" value="Diverse_substr_monoxygenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016215">
        <property type="entry name" value="NTA_MOA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03860">
        <property type="entry name" value="FMN_nitrolo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011">
        <property type="entry name" value="ALKANESULFONATE MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011:SF16">
        <property type="entry name" value="C2H2 FINGER DOMAIN TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000337">
        <property type="entry name" value="NTA_MOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="6">
        <location>
            <begin position="36"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="492" mass="55526" checksum="0555DE2EFE5390E1" modified="2017-01-18" version="1">MTSANKRQKTARKPLILNFFEHAGPAQMFPGIFAHPKDESSTYKSRAYWIKLAKIAERGKINALFIGDTLSPYDVYQGPESVTSTAIAGAQYPTNEPTSVVPLMAEVTENLGFGLTISTLAEPPYHLARRLATLDHLSDGRIGWNVVTTFTNSAARNNLNGQDLPKSEDRYDRAHEYVDVIYELLLSSWRDDAVLNDKERRIFADPDAIRQVQFKGKYFNVPGYQFTEPSPQRLPVIIQAGASGSGQKFGARIAELVFINEETPEKLKEKIQAIRKIAKDEYGRDPEHLKFISLINVYVAKTSEEAKEKFKDITSYVSKDGVQVLFGGWTGVDLSKYDYDEDLRQVGKNDPRSHLKTLLGAPNKVTRRDLIEKISGIKSNTTFVGSVEEVADQIEDLVDRSGIDGFNFAYNLWPGSFEDIVDLLIPELRRRGLAWDDYPVKGGTFRENIYGIKGQTFVPEDHGAYKYKWNAGITKEQFEKDLNESQTEHFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB35</accession>
    <name>A0A1E3PB35_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">D-serine dehydratase</fullName>
            <ecNumber evidence="11">4.3.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">D-serine deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_82199</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the conversion of D-serine to pyruvate and ammonia. May play a role in D-serine detoxification.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-serine = pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13977"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35247"/>
            <dbReference type="EC" id="4.3.1.18"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:13978"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DSD1 family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.18" evidence="11"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041294.1">
        <property type="nucleotide sequence ID" value="XM_019185348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB35"/>
    <dbReference type="STRING" id="683960.A0A1E3PB35"/>
    <dbReference type="GeneID" id="30202594"/>
    <dbReference type="OrthoDB" id="20198at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008721">
        <property type="term" value="F:D-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036088">
        <property type="term" value="P:D-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000016">
        <property type="entry name" value="D-serine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.20">
        <property type="entry name" value="D-serine dehydratase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051466">
        <property type="entry name" value="D-amino_acid_metab_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026956">
        <property type="entry name" value="D-ser_dehydrat-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042208">
        <property type="entry name" value="D-ser_dehydrat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28004:SF2">
        <property type="entry name" value="D-SERINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28004">
        <property type="entry name" value="ZGC:162816-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14031">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01119">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0216">Detoxification</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="D-serine dehydratase-like" evidence="14">
        <location>
            <begin position="317"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00075219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ62087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="437" mass="49013" checksum="CF114270167FC0D1" modified="2017-01-18" version="1">MTFLSPLIAYPAPQIKETLLEHSKGKSIDSLQTPALLINKSVVQDNCTRLLKRVDHLGYRFRAHVKTHKTIEITKQQLGYDLPNYEGRKYDSIVVSTLKEAYSVVNYQESTGEIFINDIVYGLPNIAEDTLFHILELSKKVKHFRLLIDNVEHIKILKAFNDKYNVEAPWSIFIKLDAGTARAGIEKPQVLSHVLSKVLSNPYLSLYGFYVHAGHSYSSNNVEESEKHFLEELQCVSDAVNLLEQIAPELNFKDIVVSVGATPTLHSLEHDLYTGTSKKIEDLLSNLKATLEVHAGNYGLCDLQQVSTGCVNTGNVGLHVLTTVISQYPQRKHPIGELLTNSGVIALSREPSPKFPGFGRVVTDPKYGEWEVNRLSQEHGILRPVGKSLEEGKLIPIGTKVKIIPNHACITANSFNAYYVLDDKETIIDVYIPWRGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBM8</accession>
    <name>A0A1E3PBM8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 14">6-phosphogluconate dehydrogenase, decarboxylating</fullName>
            <ecNumber evidence="10 14">1.1.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_60852</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10 14">
            <text>6-phospho-D-gluconate + NADP(+) = D-ribulose 5-phosphate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:10116"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="1.1.1.44"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10 14">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10 14">Belongs to the 6-phosphogluconate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.44" evidence="10 14"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042012.1">
        <property type="nucleotide sequence ID" value="XM_019183067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBM8"/>
    <dbReference type="STRING" id="683960.A0A1E3PBM8"/>
    <dbReference type="GeneID" id="30200313"/>
    <dbReference type="OrthoDB" id="434986at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00410"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004616">
        <property type="term" value="F:phosphogluconate dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019521">
        <property type="term" value="P:D-gluconate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000002">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.320:FF:000002">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000007">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006114">
        <property type="entry name" value="6PGDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006113">
        <property type="entry name" value="6PGDH_Gnd/GntZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006184">
        <property type="entry name" value="6PGdom_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006183">
        <property type="entry name" value="Pgluconate_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00873">
        <property type="entry name" value="gnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006765">
        <property type="entry name" value="PRK09287.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11811">
        <property type="entry name" value="6-PHOSPHOGLUCONATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00393">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000109">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00076">
        <property type="entry name" value="6PGDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01350">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00461">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0311">Gluconate utilization</keyword>
    <keyword evidence="5 10 14" id="KW-0521">NADP</keyword>
    <keyword evidence="6 10 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 10 14" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase C-terminal" evidence="15">
        <location>
            <begin position="181"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="35"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="77"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <begin position="131"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <begin position="188"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ62805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="492" mass="54041" checksum="763C10EBA2429FEA" modified="2017-01-18" version="1">MSAPTGDIGLIGLAVMGQNLILNAADHGFTVVAYNRTVSKVDHFLDNEAKGKSIIGAHSIQELVDNLKRPRRIILLVKAGQAVDAFIEQLLPYLEKDDIIIDGGNSHFPDSNRRYEELKQKGIHFVGSGVSGGEEGARYGPSLMPGGAEEAWPHIKDIFQSIAAKSDGEPCCDWVGPAGSGHYVKMVHNGIEYGDMQLISEAYDIMKRVGGFTNKEIGDVLATWNKGVLDSFLIEITRDIMYFNDDDGTPLLEKILDSAGQKGTGKWTAINALDLGMPVTLIGEAVFARCLSSIKSERVAASKILPGPQIPKDAVKNRQEFIDDLEQALYASKIISYAQGFMLIREAAKEYGWKLNNPAIALMWRGGCIIRSVFLAEITSAYRENPDLENLLFNKFFSDAITKAQSGWRKTIGLTTQYGIPTPAFSTALAFYDGYRSAKLPANLLQAQRDYFGAHTFRILPEFANDKYKVDQAIHINWTGRGGEVSASTYDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBN2</accession>
    <name>A0A1E3PBN2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_76879</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041917.1">
        <property type="nucleotide sequence ID" value="XM_019184458.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3PBN2"/>
    <dbReference type="GeneID" id="30201704"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057283">
        <property type="entry name" value="RGF3_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052233">
        <property type="entry name" value="Rho-type_GEFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572">
        <property type="entry name" value="RHO1 GDP-GTP EXCHANGE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572:SF1">
        <property type="entry name" value="RHO1 GUANINE NUCLEOTIDE EXCHANGE FACTOR TUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23582">
        <property type="entry name" value="WHD_RGF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="4">
        <location>
            <begin position="597"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="826"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="5">
        <location>
            <begin position="995"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="91"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="248"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="375"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="120"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="162"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="270"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="308"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="398"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1322" mass="150471" checksum="840AE4A553F6B671" modified="2017-01-18" version="1">MSFQRNGFGDNQQRAPYPDTELAWDPVESLEEFNKSTYSSYIQHLDSVPLDTAPPIPGPPLPTRPPQHIIDQHISSINQKEQFQSFQRTPFINTNTAPNSTSLQSTPSTSRVRGPRQLPNKFSNPNLSASINSSPGYGVRQEWPQINYNPGGFNTPPRPDPFNSSPQRSMPNSSPFMNQNESSFMSSPIQIQPLNSSPVQQIHQSPITGPRPFPFSSPSYNQQPPSPTSQRRLPLKVNTISNQYSIQNDTSFMDSPTSAYTQKLPDIPTLTPSTMQESPVQFNTDQQRTSRNSPSPTKGLAGPRLMPSMSSHSTLKISPSSSPTKVDYYDHRTSWNSAYSLNIIPADENDDLDSISAPPSAVEIVPEVPNVSTIPPIPRTTSLFDPDSIPIPDEPPQRGSTITSRQTGGEKGLPPLPDSLNLPSLPFSSYSLMASHFKECENIQLLSELFKWSVSLVNEWSEGLNISKNEYKRSLKLMISNNSPKLNNFIIDANIETILNSFEKQNAIYFDHNDNVHFMDNVNVSGVLPQLTGCYSKAHNISSLYQCYSPRCSLTIYQPPVPTVPAKESGVAKLGEWTSYWNITEQDLVEMDESEVKKQSHIFELIRQQQNIINLGEIQIREYGQSFKNANPQLLPDVSKFYNDAFNSVKPLIDIHKKHLLEPLLHRINTQGKFITNIGELFLNWATAATVPYLKYTEKLATVRELIKFEKNRQSRFAEWLYKVDQNPKVMEASLDHNRIFFSGFIGHTQLLSLALNSVYKKTRENDMDHVLLKNAIDEINKLNRKIDEMQDSALQNRHLRLLAGRLTWKNNVLANDLKLSEPNRRLIKEGAVTRKREKWITSNYYLILLDNYLLITEPQKDEHYKISEKPIPIEFLQIETKNFASASNVNLSTEGEIYPFKVRYTGQNVSYTFYAETLAERDSWFHSINQVQSKKVKNSIFEPFKISILADQFAYEEGEQPQKLPLLAAGSNIDVALKSIEADKSTDVTRPLMFSEVSCGTTFHFQDRSFYVLGLNFGLYITEAENPKGWKRVLELGKITQVEQLDSLMVILADKALYYFNIVSLLLNYYDQNYDGQTVGEKLSKRDVSFFKIGTYYNTKLLFLMKSSISTVGSRFKVYTPIMDTFGVFQYFQLYKRFQYASDCFGISIFNSMFVLHTSKGFEILSFQILNESQPIPKFIEYLKKPKTELDLIKKKLNSSSAKPLTMVKVFQKPQFYLIYDSFAIVIDTIGQLISNNFIFPFKFKCSKVCLKENFLICIGEDIVEIFDLNYDDALGFQKFDPVQIITGKDIRLIDGIDAKIVMAHPKITGRQLVITLDRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBP7</accession>
    <name>A0A1E3PBP7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_15058</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042050.1">
        <property type="nucleotide sequence ID" value="XM_019180627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBP7"/>
    <dbReference type="STRING" id="683960.A0A1E3PBP7"/>
    <dbReference type="GeneID" id="30197873"/>
    <dbReference type="OrthoDB" id="278280at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032378">
        <property type="entry name" value="ZC3H15/TMA46_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681:SF0">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681">
        <property type="entry name" value="ZINC FINGER-CONTAINING PROTEIN P48ZNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16543">
        <property type="entry name" value="DFRP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="165"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="165"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="41"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="310"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="332" mass="38378" checksum="29179EF286C49E47" modified="2017-01-18" version="1" fragment="single">MPPKKGKQKEPAPKKDAKKNAADKTFGMKNKNRSTKVQQYIKQVETQVSEAQMKKNAAMAKRKAEEKKAAELAKMEAQRLLNMQVEQKVPFGVDPKTVLCVNFKNGNCPRGAKCKFSHDMNVGKKATKKDLYTDDRALKEADTMDKWDEEKLRSVIKSKHGNPKTTTDKVCKFFIEAVENGKYGWFWVCPNGGTECKYRHSLPEGFVLKTKEQKRMEKMELESQPKITLEEFLETERHKLPKTGLTPITVETFARWKENNKIKKLNDKEKDGKKLTGREIIEKKFLDKNYYENEDDASQAFDLSEFRKELEPEDDNIKDYGDGKNVSFETKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBS3</accession>
    <name>A0A1E3PBS3_WICAA</name>
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        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_60471</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3PBS3"/>
    <dbReference type="GeneID" id="30200244"/>
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    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
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    </dbReference>
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    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="151"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="378" mass="43636" checksum="B0C11427072BBA11" modified="2017-01-18" version="1">MEFSSAKGILVVDPIEARNICIKRASSNVYCLFQIDNRVYRSKTTAFKVPSLPRWKEDSQCRFDIDPGVTPLLKVFVLEKTNGLPLVVGKGELNLIDIFYEKKSDRWVKLSPMGQLDLELTFYQAAPMLPRKTLPNPKVPPKHPQREGEIKENLKQKVSSVDQVFEEENREERHRLRQLAKKVKRRYRESRSSREFVMKEQLKKMTNDELLYVEVNQKIEKALPDVPGDDSVKIEENIPDINVLSIDTESISQIDLNKLPFSADSIGTHMITKLDELKAQQEKQKEEDSKKMYTPLSTEAFSIISRLERGRARPKDLQVEGGASDYKGDGTWGKGRLTDAAYTGERPRLPPKIPYGMNAEEYYMLQRREYVDYFKTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBW0</accession>
    <name>A0A1E3PBW0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL5m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_24788</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL5 family.</text>
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    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019181057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBW0"/>
    <dbReference type="STRING" id="683960.A0A1E3PBW0"/>
    <dbReference type="GeneID" id="30198303"/>
    <dbReference type="OrthoDB" id="539541at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="50S ribosomal protein L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_uL5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL5 N-terminal" evidence="6">
        <location>
            <begin position="138"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL5 C-terminal" evidence="7">
        <location>
            <begin position="195"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="101"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="299" mass="33714" checksum="3E0C8A8E50AFC16E" modified="2017-01-18" version="1">MFSGKVVASHGGARLFSTTSAASGRVGCSTVKPVHHLVKIDKNKLSPRFPELNLEKNDIRNPAFRPVATHQDRVEEYYKNSIQSDLLLINFQHGERRQLGLKRRQWDGSSPNHPSRSLRKPQGSAVETTDYKPHTTKNIPRIESLVINCYARAARENSNHAITAALQLQQITNAKPKVIHSRSDVIAWKLRKGWPMGAKVELKGRPLSQFLATLTEIVFPRIREFRGLTVRSGDKNGNITFGLTPEDIKFFPEIESNQDNWPFTYGMTMTIKTTGQTDGEARTLLSAYGFPFYGTERKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBW6</accession>
    <name>A0A1E3PBW6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_89102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042102.1">
        <property type="nucleotide sequence ID" value="XM_019186174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBW6"/>
    <dbReference type="GeneID" id="30203420"/>
    <dbReference type="OrthoDB" id="3997547at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="211" mass="24478" checksum="28F39E4F5607F5A8" modified="2017-01-18" version="1">MFVEHSGPRADRILSADVSMRENTRLFSPKSLSSSFKIHASPKRRNSEDFDHMKRTKSDELELVKLKHDNDITSCHGNDWPVFIHPEGGYIQQSPYGFIHIVKDENSERSSIDNTINLETFTTKLPALKANTPQKKSTKQKIPLPYSRLPLPYSPSGEIENYMVEGYEEEHNKSYDHHKEDIVNVNYNLYDQHTEDAHLENEKSEDFDMIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC38</accession>
    <name>A0A1E3PC38_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Glutamate decarboxylase</fullName>
            <ecNumber evidence="3 9">4.1.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_60915</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 9">
            <text>L-glutamate + H(+) = 4-aminobutanoate + CO2</text>
            <dbReference type="Rhea" id="RHEA:17785"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="4.1.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.15" evidence="3 9"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042077.1">
        <property type="nucleotide sequence ID" value="XM_019183081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC38"/>
    <dbReference type="STRING" id="683960.A0A1E3PC38"/>
    <dbReference type="GeneID" id="30200327"/>
    <dbReference type="OrthoDB" id="5152799at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004351">
        <property type="term" value="F:glutamate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000017">
        <property type="entry name" value="Glutamate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010107">
        <property type="entry name" value="Glutamate_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01788">
        <property type="entry name" value="Glu-decarb-GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321:SF3">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="522"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="531"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="544" mass="62144" checksum="895B40569212FFF6" modified="2017-01-18" version="1">MLTKLIDPEKLEDTILSNSHYHNPNNRKDDTEFVSQNKYQLPENGLSEELTYQLLHNDLSLDGNPTLNLASFVNTFSTETAKELAKENIVKNLADADEYPTLMDLTQRNVSILGKLWNRTSEEPVGTATTGSSEAIMLGGLAMKKIWQERQKAAGKDYYKPNILMGSNAQVALEKFARYFDVEARLIPVSEKSNHVLDLTKIEENIDENTIGIFVILGSTYTGAFEDVEQVSKILDKIEEEKGIYVPIHVDGASGAFVAPFIYPELKWDFRIPRVVSINTSGHKFGLTTAGLGWIIWKDKKHLPEGLIFKLRYLGSVEESYTLNFSRPGYQSIHQYYNFLHLGSKGYREIHHRSLKNARVLSNFLEKTQYFECISNIHRKKGQLTFDPSLSREELANEDNEDSYYNKGLPVVAFKFRDEFKQQYPHIPQALLSTLLRKRGWIIPNYPLPPNEEATEILRVVVRYELTDDLLFRLISDILKTTQALIKSSIFYQQSVDATSAADKNRYIYDLLTSIASGGEEDINSHEKKQDHKKNHHTTFKGTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCR9</accession>
    <name>A0A1E3PCR9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 9">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="1 9">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 9">Tyrosyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_82891</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="1 9"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042211.1">
        <property type="nucleotide sequence ID" value="XM_019185457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCR9"/>
    <dbReference type="STRING" id="683960.A0A1E3PCR9"/>
    <dbReference type="GeneID" id="30202703"/>
    <dbReference type="OrthoDB" id="337870at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070184">
        <property type="term" value="P:mitochondrial tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000001">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02006">
        <property type="entry name" value="Tyr_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024088">
        <property type="entry name" value="Tyr-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024107">
        <property type="entry name" value="Tyr-tRNA-ligase_bac_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766:SF0">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766">
        <property type="entry name" value="TYROSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ63004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="480" mass="53961" checksum="EFEED0977DAB8C6E" modified="2017-01-18" version="1">MRLLKPHSLNFLVDQNELKKDLEEPLMAHLNKRGLISSTTSEELEKVLEKTKLGLYCGADPTAKSLHLGNLLPLLILLHFNLRGHHITGLVGGATGAVGDPSGRTTERATMEEQTRLDNVSKIKQQMEHFFNNGVRYVKYLSTSKVIEEGRIQSKNNYDWWKDVTLLEFLAKYGRHIRVGQMLARDSVKGRLESENGIGFNEFAYQILQAYDFWHLFRNENVSIQVGGNDQWGNITAGIDFITRLKANLKENNKKDKEIQRLTGRSSFGVTVPLLTTASGEKFGKSAGNAVFIDPTVTPSFDLYQYFIKTPDSEVEKLLKIFTFLPLKQIEEIITKHNEDPTIRSAQRILANEVTDLIHGVGSGKDAEIVSSILYPLPEDPYPDITSDELISSFKAANVLKVLPKEEFIGKPYSAILSKVQDISRNEARKLITSGGVYQGYERIRIDTSDSMLIGDEQLIDGKVLLLRVGKGKYHVIQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU50</accession>
    <name>A0A1E3NU50_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_98195</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454216">
        <property type="protein sequence ID" value="ODQ56711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035918.1">
        <property type="nucleotide sequence ID" value="XM_019186643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU50"/>
    <dbReference type="GeneID" id="30203889"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ56711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="75" mass="8521" checksum="1D05CCE2413BF05B" modified="2017-01-18" version="1">MTVEVVKPWTGSLDRYRCPLSIKINLITRVLVVVGGGARCTLVLKRLTSRDYKLMLNLSIDLQSSQNLESLFCYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU75</accession>
    <name>A0A1E3NU75_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription initiation factor IIE subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_65623</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TFIIE beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035948.1">
        <property type="nucleotide sequence ID" value="XM_019183952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU75"/>
    <dbReference type="STRING" id="683960.A0A1E3NU75"/>
    <dbReference type="GeneID" id="30201198"/>
    <dbReference type="OrthoDB" id="5323195at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005673">
        <property type="term" value="C:transcription factor TFIIE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001097">
        <property type="term" value="F:TFIIH-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054600">
        <property type="entry name" value="TFA2_E-tether"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040501">
        <property type="entry name" value="TFA2_Winged_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016656">
        <property type="entry name" value="TFIIE-bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003166">
        <property type="entry name" value="TFIIE_bsu_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12716:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12716">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22254">
        <property type="entry name" value="TFA2_E-tether"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18121">
        <property type="entry name" value="TFA2_Winged_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02186">
        <property type="entry name" value="TFIIE_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016398">
        <property type="entry name" value="TFIIE-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51351">
        <property type="entry name" value="TFIIE_BETA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIE beta" evidence="9">
        <location>
            <begin position="73"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="19"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="237"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR016398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ56741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="291" mass="32351" checksum="B6B179B65A31B80C" modified="2017-01-18" version="1">MSNPLLANLNAFKNKVKSAPVLSSARSKAQTPPASAKSNNTKPSSSSSSNSLKRSAGDDYDDDFDGEHSIKNKKPAYDASGSHLSTKLLLAVDYIKERSKPVHIDALLSYLSLNNDEQKSKLLSLIKNLDKIQYDPNENTLEYVSIHNIKNKDELLNYLREQPTFKGISVKELKDGWNDCIQSINELENEDKILVLRTKKDNHPRLVWANVGGELGGVDDEFVKAWQNVKLPDRSELPGLLNKRGLKPASVDPATVKSATNNEPTKKRKQRKGKITNTHMTGILKDYSNGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUA3</accession>
    <name>A0A1E3NUA3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBM1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_71465</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454216">
        <property type="protein sequence ID" value="ODQ56706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035913.1">
        <property type="nucleotide sequence ID" value="XM_019184248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUA3"/>
    <dbReference type="STRING" id="683960.A0A1E3NUA3"/>
    <dbReference type="GeneID" id="30201494"/>
    <dbReference type="OrthoDB" id="3978949at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030248">
        <property type="term" value="F:cellulose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000254">
        <property type="entry name" value="CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035971">
        <property type="entry name" value="CBD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00734">
        <property type="entry name" value="CBM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00236">
        <property type="entry name" value="fCBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57180">
        <property type="entry name" value="Cellulose-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00562">
        <property type="entry name" value="CBM1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51164">
        <property type="entry name" value="CBM1_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="47"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="CBM1" evidence="3">
        <location>
            <begin position="128"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="CBM1" evidence="3">
        <location>
            <begin position="286"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="16"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="90"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="221"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="547"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="16"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="221"/>
            <end position="245"/>
        </location>
    </feature>
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        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
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            <begin position="547"/>
            <end position="668"/>
        </location>
    </feature>
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            <end position="718"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ56706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="718" mass="73275" checksum="5C7E24D80F0EC517" modified="2017-01-18" version="1" fragment="single">MNDYYYQCVPGTATTPASKPTTAATSTTSTQKTTSSTSTSTSKSNQNCVSAYGQCGGANYGSPSCCTDGYYCSSMNDYYYQCVPGTATSTSTSTSTTTSAKQPTTTSTSSTSTSSTTTSKPTTASATQCVSDYGQCGGVNYVGPNCCGNGFACSSMNDYYYQCVPSDATSYSRATSTLSSKLPTDTFTSKITSSSNGLVFSSTTTTESSIILASSSASKSSSLTSSVVPHSSATSDTSTHTPASHQNSESSKNSPSTISATTITLSGTTSSSNDETSSLVSTSAEICVTASGRCGGKGYYGPSCCVQGWSCRSIDEYNYQCVEEVTTSSNAQPSSATPSSSSIIKPTSIIVSYSDNYSASPSSTTSERPSTTSEGSFTSSTFVSSQITLPPSSTTAEFSTHTTTLSQYTTYRQNTNYRVLIYLVDTDGTTYKVREEYANTSMQQQTTIATYYPVTTSLTSISSIGSISQIESSFISPGNTGSSGYSSVASSSELSRVSSAPVSSSDYSSVASSSEWSSASSGSSAPVSSSDYSSIASSSEWYSASSSSSAPVSSSDYSSIASSPESSSASSGSSAPVSSSDYSSIAPSSEWSSASSGSSAPASSSDYSSIGSSSEWYSASSSSSASVSSSDYSSIASTSEWSSALSGSSDYSSIASSSERSTVSSDYSKVTTVKPGETTASLDLSSSSDYSSVASSSEWSSASFVSSAPVSSSDYSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUC0</accession>
    <name>A0A1E3NUC0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_81563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019185255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUC0"/>
    <dbReference type="GeneID" id="30202501"/>
    <dbReference type="OrthoDB" id="10552735at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ56728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="224" mass="25467" checksum="CBAF44AEDA081CCE" modified="2017-01-18" version="1">MSRQVNPNHSAEEFESFNSNYSKYDVSKKSNFQNIYSEEIAIESNTANIFQSINRINQLINHSESLSTEINEKFAELQLVNQDVEENLKNISIPKTIPTTIHYDSKPKNQYLPTPTDDYDLGKPSIGTTDPYDIPSSPDLSFNGGGSDIDEVEIEINDKISRMVEKDYNSENILKLKNIVNSTFDVLTYGKDDNERLLMNTSDDIKNKSELLIDKLHGLVESFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUD3</accession>
    <name>A0A1E3NUD3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_98187</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454216">
        <property type="protein sequence ID" value="ODQ56694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035901.1">
        <property type="nucleotide sequence ID" value="XM_019186639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUD3"/>
    <dbReference type="STRING" id="683960.A0A1E3NUD3"/>
    <dbReference type="GeneID" id="30203885"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001123">
        <property type="entry name" value="CK1/CK1/CK1-D protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ56694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="251" mass="29168" checksum="A9C0613AE30F5FE1" modified="2017-01-18" version="1" fragment="single">MINQVPVAIKFEPRKTEAPQLRDEYRTYKHLNGSYGIPNAYYFGQEGLHNILVIDLLGPSLEDLFDWCGRRFSVKTTVQVAVQMISLIETLHAHDLIYRDIKPDNFLIGRPGQPDENQIHLVDFGMAKQYRDPRTKQHIPYREKKSLSGTARYMSINTHLGREQSRRDDLEALGHVFFYFLRGQLPWQGLKAPTNKQKYEKIGEKKRTTALNDLSIGLPHQFATYLETVRNLNFEETPNYEGYRNLLLSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUG3</accession>
    <name>A0A1E3NUG3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">MICOS complex subunit MIC12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Altered inheritance of mitochondria protein 5, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10 12">Found in mitochondrial proteome protein 51</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_81779</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Mitochondrion inner membrane</location>
            <topology evidence="2 12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the MICOS complex subunit Mic12 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454220">
        <property type="protein sequence ID" value="ODQ56670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035877.1">
        <property type="nucleotide sequence ID" value="XM_019185294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUG3"/>
    <dbReference type="GeneID" id="30202540"/>
    <dbReference type="OrthoDB" id="4037694at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044284">
        <property type="term" value="C:mitochondrial crista junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031463">
        <property type="entry name" value="Mic12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17050">
        <property type="entry name" value="AIM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133525" description="MICOS complex subunit MIC12" evidence="13">
        <location>
            <begin position="20"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ56670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="110" mass="12559" checksum="1DBED65D7E76A8BC" modified="2017-01-18" version="1">MGNRIHGFLAGVLLTTSVAYYTSQEFKRSQEFVSKTLRDTESIIQNHGKEPQPVPRSVEFQYRPSIKQTVADLWNEQILSTVRSIYSFDVERFTSSITKSVQDGVNNISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUY2</accession>
    <name>A0A1E3NUY2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LYC1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_36708</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036111.1">
        <property type="nucleotide sequence ID" value="XM_019182460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUY2"/>
    <dbReference type="STRING" id="683960.A0A1E3NUY2"/>
    <dbReference type="GeneID" id="30199706"/>
    <dbReference type="OrthoDB" id="2020070at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055100">
        <property type="entry name" value="GNAT_LYC1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053013">
        <property type="entry name" value="LAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34815">
        <property type="entry name" value="LYSINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34815:SF2">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13527">
        <property type="entry name" value="Acetyltransf_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22998">
        <property type="entry name" value="GNAT_LYC1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LYC1 C-terminal" evidence="1">
        <location>
            <begin position="177"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ56904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="372" mass="43299" checksum="149D97B5B186AF91" modified="2017-01-18" version="1" fragment="single">TAMTKNSDLVLELISDPELIRFTHLENSEAWRSKLTKEQYADREWTIGCQSSLGKRKESQNLGVFHYVLRDLSIKAENNHDNIVASCETMNRIAWRIDGKEGELTEVICACIGGVFTLPNHRKKGYAAEMINRLNDALDRQLGSKSFTFLYSEVGEYYSKFGYDSFEVPTHSIPTENVSSTKVPKAHYHFLKYDDYQKLVESQYSHVKQDLIQNACDTDKTLVTLAPDIDIFSWFHDRDIFISKILRPEIQLKHFGAVLNQTNDHIIWLHDWNEEKLVIVRIYNENEHDLEAFKKLINIALEEAQLYNFKEITLWDSSLGGEKHHDEAFEYIRGLKGVKTLQPNSSVSAIRLHNKTQKGDYLWLNNDKWCWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUY8</accession>
    <name>A0A1E3NUY8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_65276</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036218.1">
        <property type="nucleotide sequence ID" value="XM_019183885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUY8"/>
    <dbReference type="GeneID" id="30201131"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="579" mass="66393" checksum="B5166AB465F412D8" modified="2017-01-18" version="1">MPSLLSIPLEVLESISYWLNPSDLLTIAQIKELEPIWRNWSILKYSNSSFTSPDSITCHVNNMKYKSKKRKITPQLVLVILDVYDDPFEKFAEHLLGKFPHHLSILAKNEEVLAKFLISLKPMADSKFFNIDSVEIKGSDLYLDYKTIPFYAPEAQYLELYNVEFIKKHNQKVVSPLLEKLKVTGLNDPDVLKFPATTTLIRPGESLLTSSRFVDLLEGVETLKIEHLYDSGGLDGLNLSTLVNLTIGVDNVLNFSRIKAPMLKNLKILYCEQFVTISDCEFDSLETIEANNCLIGECQRNFAPKFTKLKLKPNFYPIVQEINHGGFFDQVKELTTCNDYFISMVNLKKLEFLHICEVENGAVGELSFVKCPSLKTLCLSYVDFDEKLKPNAPNLENLSMVKCNINYDIFNNIPILLPKLKVLKFEGRIYYYNRIEGTLGNLEWKFSNLKLSELETLIFGNKTESYSRRGIFRTLVTKIGFYNCDFPKLKLFSVDHWSHDFKLINLQIGAPNLKFLKLRSPGTGELDLSNYNHLKALSIQDVSILKYPNCESLEFIGVYACEFKFLHIGELTNLIEVNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV46</accession>
    <name>A0A1E3NV46_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Exoribonuclease phosphorolytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_65791</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036117.1">
        <property type="nucleotide sequence ID" value="XM_019183986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV46"/>
    <dbReference type="STRING" id="683960.A0A1E3NV46"/>
    <dbReference type="GeneID" id="30201232"/>
    <dbReference type="OrthoDB" id="2504340at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF2">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT MTR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="9">
        <location>
            <begin position="48"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ56910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="236" mass="26048" checksum="7FAEBE33238F49A1" modified="2017-01-18" version="1">MSNTIDRRRLLGPPNAKPLIFAPLSEKDQQKTLIDKTKEDDIEAQISRTFIKTGLITNANGSSFIEVDGNIISVSVYGPRPIRGSFVEKTTLSVHLEDSTDLFSDLLNKKFCNYIENNFLSVINLSKYPKSGIDIFINIISVQDAEKLYLKTLSLVSDATTLALVNAGIEIIDLVVSGFDLNNNSVLSFIKTNEIVGLLSESEDSLDNLSEVIRKTQKKSAVVKNALTTYLISNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVC8</accession>
    <name>A0A1E3NVC8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Histone deacetylase</fullName>
            <ecNumber evidence="2 10">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_81273</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17 10">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="2 10"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036251.1">
        <property type="nucleotide sequence ID" value="XM_019185207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVC8"/>
    <dbReference type="STRING" id="683960.A0A1E3NVC8"/>
    <dbReference type="GeneID" id="30202453"/>
    <dbReference type="OrthoDB" id="1918432at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032221">
        <property type="term" value="C:Rpd3S complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070211">
        <property type="term" value="C:Snt2C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061188">
        <property type="term" value="P:negative regulation of rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045128">
        <property type="term" value="P:negative regulation of reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061186">
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        <property type="term" value="P:positive regulation of macroautophagy"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:protein localization to nucleolar rDNA repeats"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:rDNA chromatin condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030174">
        <property type="term" value="P:regulation of DNA-templated DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10004">
        <property type="entry name" value="RPD3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
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        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF10">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037913">
        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="14">
        <location>
            <begin position="41"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00061569"/>
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037913"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ57044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="436" mass="49045" checksum="2411D48B4BD5D3CD" modified="2017-01-18" version="1">MATSNYAQKPFDPITVKPNDKKRVAYFYDADVGNYAYGAGHPMKPHRIRMAHSLIMNYGLYKKMEIYRAKPATKQEMCQFHTDEYIDFLSRVTPDNLDMFQKESVKFNVGDDCPVFDGLYEFCGISGGGSMEGAARLNRGKCDIAINYAGGLHHAKKSEASGFCYLNDIVLGIIELLRYHPRVLYIDIDVHHGDGVEEAFYTTDRVMTCSFHKYGEFFPGTGELRDTGVGKGKNYAVNVPLRDGIDDATYKSVFEPVISKIMEWYQPSAIVLQCGGDSLSGDRLGCFNLSMKGHANCVNFCKSLNVPLMILGGGGYTMRNVARTWAFETGLLNSELLDTDLPYNDYYEYYGPDYKLDVRNSNMYNANSPEYLDKILTQIFSNLEHTKFAPSVQINDVPKDAEDLGDEDEDLPDAIDTKGGSQYARDNLITPKTEFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVL5</accession>
    <name>A0A1E3NVL5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Replication factor C subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_13861</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019180586.1"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="446168at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902983">
        <property type="term" value="P:DNA strand elongation involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BRCT_RFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Replication factor C subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Replication factor C subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000001">
        <property type="entry name" value="Replication factor C subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Replication factor C subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013725">
        <property type="entry name" value="DNA_replication_fac_RFC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012178">
        <property type="entry name" value="RFC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF6">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25361">
        <property type="entry name" value="AAA_lid_RFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08519">
        <property type="entry name" value="RFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036578">
        <property type="entry name" value="RFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="11">
        <location>
            <begin position="5"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="83"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="620"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="620"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="651"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ57249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="665" mass="73465" checksum="579DB5E433452315" modified="2017-01-18" version="1" fragment="single">EIPEGAPNCLTGLTMVFTGVLPNLPRDDAENLAKKYGAKVTKSISSKTSVVVLGEEAGPSKVRKIKQLKIKAITEDGFIQLIKGMPEEGGGGAEAEKARAKREEEERKALEESVRLQEEEEAAAKKREAAIKKAQASGDYASTSKLKTDAEKLWTVRYAPTSTSQICGNKSSVTKLQTWLEQWPAKFGNRKPAKGESEMRAVLIHGPPGIGKTTAAHLVAKSLGYDILEKNASDVRSKGLLNAGVGNILNNSSVMGFFNPSESKNSNGDKFCLIMDEVDGMSGGDRGGVGQLASYCRTTQAPIILICNDKSLPKMRPFDRVTLDIPFRRPSAKEMKSRLMTIALREKIKLDPNIIDQLVQATSNDIRQIINLLSTVSKTQKNIDSENSNDISKAWQKNIALKPFDIIPRLLSGSNYSKNSQVPLYKKMEYYFDDHAFVPLMLQENYLNSKPANGGSKLKHLELVANAAESISQGDLVDRKIHSAEQQWSLMPLHAIMSTVRPASFVAGSLGGRVNFTAWLGQNSKAGKYYRLLTELQYRSRLRTSTDQVEFRLEYIPVLAKKLLDPLVEGGADSIPDIIEILDHYYLNKADWDFLMEFPIGEDVTQAKLKKIPTATKSKFTRKYNSSSHPISIYRPGLSTGVSKPKKDAPDLDDVVEDDPEPEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVR1</accession>
    <name>A0A1E3NVR1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_97592</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036287.1">
        <property type="nucleotide sequence ID" value="XM_019186598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVR1"/>
    <dbReference type="GeneID" id="30203844"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="56" mass="6571" checksum="469BDB787787C6F1" modified="2017-01-18" version="1">MTVFITSKNPRSIKSSRYHNPLQYQNNLSKMAQSLYLKLEYKFIISESKTKPKTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVV6</accession>
    <name>A0A1E3NVV6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_86261</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">High-conductance magnesium-selective channel that mediates the influx of magnesium into the mitochondrial matrix. Essential for the splicing of mRNA group II introns in mitochondria by affecting mitochondrial magnesium concentrations, which are critical for group II intron splicing. It also suppresses a variety of mitochondrial intron mutations and its absence may disturb the assembly of mitochondrial membrane complexes.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Mitochondrial inner membrane magnesium transporter required for mitochondrial magnesium homeostasis. Modulates the conductance of the MRS2 channel. Involved in the splicing of mRNA group II introns in mitochondria by affecting mitochondrial magnesium concentrations, which are critical for group II intron splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Forms homooligomers. Interacts with MRS2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homopentamer. Forms homooligomers. Interacts with MFM1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Mitochondrion inner membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036029.1">
        <property type="nucleotide sequence ID" value="XM_019186024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVV6"/>
    <dbReference type="GeneID" id="30203270"/>
    <dbReference type="OrthoDB" id="10251508at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045016">
        <property type="term" value="P:mitochondrial magnesium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12823">
        <property type="entry name" value="Mrs2_Mfm1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000005">
        <property type="entry name" value="Inner membrane magnesium transporter MRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.330:FF:000002">
        <property type="entry name" value="Inner membrane magnesium transporter mrs2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039204">
        <property type="entry name" value="MRS2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890:SF27">
        <property type="entry name" value="MAGNESIUM TRANSPORTER MRS2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890">
        <property type="entry name" value="RNA SPLICING PROTEIN MRS2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22099">
        <property type="entry name" value="MRS2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 16" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="364"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU366042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ56822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="426" mass="48494" checksum="3DA225740F2D5856" modified="2017-01-18" version="1">MFCSTSIRRVIFPKKNLDLTKIVKPITPNDSFVSCTVFDLNGNVIAVSKHFPKMKFLQEHGLYPRDLRKIDTSAIDIIPSIVVRDNCILVNLLHIKALVEANKVMIFDTSNPSAASKLGLFVYDLEAKLKTPSTGWVQQYEHRALESILINVMTCLETELHHHLSVCGLILAELEDQIDRDKLRDLLIKSKALSTFYQKALLIRNVLDELLENDDDLSGMYLTEKLQSTITTPPEERTDYGEVEMLLEAYYKQCDEFVQQSESLINDIKSTEEIVNIILDANRNSLMLFELKVTIYTLGFTVATLLPAFYGMNLKNFIEESQLGFGTVVGVSVILAFLVTSANFKTLRSVQKLTLMAPGTKSSAKQAFMKQTPPQPPSSSKPYASTAHKRFSLAQRFRLFRRKRLMGDQKQRDIVWKWLIEEKSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVY4</accession>
    <name>A0A1E3NVY4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_65557</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
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            <begin position="1"/>
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        </location>
    </feature>
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        <location>
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            <end position="34"/>
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    </feature>
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    </feature>
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        <location>
            <begin position="114"/>
            <end position="142"/>
        </location>
    </feature>
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        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
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            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="217" mass="23746" checksum="AA5CCBAE89DDEF3E" modified="2017-01-18" version="1">MESISSHYAAARRNKAKLASSGLDRQEIEVDKENIPSYAPHSKRTGVSEMASATKRRKTEVGAKEIHSPFKQTLSPSKGQTNLSKILNSAPQPPRETKQTRPVLKPLDRKPLKTSPSISNLSKPLPRSSTTASVKTYLNPTVSSLKSSTSSKHLSRSSTLNNLTKPTAASISKSSTTHNLAQTLHRKPSSTNLQESVSRIPRSKSTINNKNARPSWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
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                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
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            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
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        <property type="project" value="UniProtKB-SubCell"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW27</accession>
    <name>A0A1E3NW27_WICAA</name>
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        <recommendedName>
            <fullName evidence="14">DUF1640 domain-containing protein</fullName>
        </recommendedName>
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    <organism evidence="12 13">
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            <taxon>Saccharomycetes</taxon>
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        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CCDC90 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036407.1">
        <property type="nucleotide sequence ID" value="XM_019182415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW27"/>
    <dbReference type="GeneID" id="30199661"/>
    <dbReference type="OrthoDB" id="889336at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000214">
        <property type="term" value="P:regulation of L-proline metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000018">
        <property type="entry name" value="Mitochondrial protein FMP32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024461">
        <property type="entry name" value="CCDC90-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360:SF1">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07798">
        <property type="entry name" value="CCDC90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ57200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="22365" checksum="F754967AB2FF9FA4" modified="2017-01-18" version="1">MLPRIGIRNFSTFRPLRAVHFDTRKFVLQLQDEGFTPEQSEGVVRIVSSALADGVNSIGSTLVTKEILSKMTYQQKVDFAKLRGELQTIDRSEFTAIQSENERLRNDLEKLRTRIREEITKSNAGVRLDLNLEKGRIREEGSQHESHISEINTRIDQEVANMKMQIDGVKTQVMQWLIGVCTGTFALVLAYVRLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWE7</accession>
    <name>A0A1E3NWE7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_96753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036652.1">
        <property type="nucleotide sequence ID" value="XM_019186532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWE7"/>
    <dbReference type="GeneID" id="30203778"/>
    <dbReference type="OrthoDB" id="10584446at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012655897" evidence="1">
        <location>
            <begin position="16"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="290" mass="33835" checksum="EC0AEBA287310185" modified="2017-01-18" version="1" fragment="single">MRLIWCLFWVQAALCQSSYEDIFYDILPKRLQTTNVAIAASTRLYANRYVDVKDLVEDLIGDPHYVNSFDFSKPYDYAHQDYNASASKSTSELSNEIEKGLYTKLLEIYQSFQRIYSILGDMTIDDFVAIYDYIYGDIPPDEVITTLGDVDKEPDELFENMFEFNNGFEPKFEQIYPLIPFVDVDDYQELSPGSTTGDDEVQAQLLLTLIKIPLLIIKIILKIIWLLIELKKYIELIETKIILYIIKIIQKICKIIMRIKKKISKIVKDFKNLYIPYLIAKKIKAIILLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWM0</accession>
    <name>A0A1E3NWM0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_85684</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3NWM0"/>
    <dbReference type="GeneID" id="30203176"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13577">
        <property type="entry name" value="SnoaL_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="11"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="142" mass="16047" checksum="793E0933053FE7CC" modified="2017-01-18" version="1">MSSYYPIKIQYLTPREQVIDAINRLYAGFDDSNIELAASAFVHSDEIVVKLGGKVINGWTNLREEFIKNVMPVSTIHQLTNFRVIFESATTAKLTAHCVAHHSTKERGYYSRGGLAEVSLVLDEVDDLWKIVELTMKGKWVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWN7</accession>
    <name>A0A1E3NWN7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_64882</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036740.1">
        <property type="nucleotide sequence ID" value="XM_019183825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWN7"/>
    <dbReference type="GeneID" id="30201071"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="346" mass="40400" checksum="42649AB55A1A6CA3" modified="2017-01-18" version="1">MFSLSLFPREIILEIFPLLYRDDLISLLLVPEMIQAWSRWVCIVTNNTEFKDPNLIRYYKVVDIGSFLDITVYEPMVKGITILHVLKNELEPDTQNWLTLKDDAPVLSLDNLHPFTLSRGTSVSLEGEGSNYKYNLHYFMGRFKFFRKKNSQLTSINTKGMPSVVINDSSHVFPLRLLDTRRLSICYCDFGSSLTILEAPLLERLYFKFCKLSEIRFEIPRFLELECTSPKFLETAEPFNVERVRELKLTKYIDWGNLSRLNRLKNLEKLTISTSLRFASIECHIIRDVVLPNLKSLEFLHSSRIKFKNCHFPSLKEFRLIQTYIEAWSKSDSNFGNVGPDEIIVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWT8</accession>
    <name>A0A1E3NWT8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_81268</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036244.1">
        <property type="nucleotide sequence ID" value="XM_019185205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWT8"/>
    <dbReference type="STRING" id="683960.A0A1E3NWT8"/>
    <dbReference type="GeneID" id="30202451"/>
    <dbReference type="OrthoDB" id="5817083at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46283">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ57037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="458" mass="51589" checksum="DE6294D72C3275B8" modified="2017-01-18" version="1">MSEGQDRCYICLGTGDERPPLARPSDSSKLVQMCQCSLRAHARCLTDWASDLEYRARDDPQLDNNGGAMASPADGFRLGVPVAVIKDGAFKKHHIMKTRVKCPQCGVYLFIDIKPSRLLSLHNLIKGVSREITKTGLMAVVGSSIACSLAISVVGIFVSGGLQLFLSIAPESVLTKLLDFKSGRINVALSNEEVGSRQLFLLGSFPIFLYGLRSENAYLDLFSSVYPTFFFPNLVKGFSNSGPKIFLLFSEPLKRIYSLFYTMTFNRLYYSWTKLVKPIYLADRLSLEELETFERESQDKITLKENSKSQNIFQRLYSCLFGTSDEERAAIRKRLWTETKAIFSRDYSTVFHKRSRFMSILTTILWPILGAKLGKLLLKVVKINTFCNKYSSTPDEATYIANLMGSVLLVLVKDLFNLYIAWLNVKQLLEVSVVRASFGPEDPSNEWAQGSVMFPFRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX28</accession>
    <name>A0A1E3NX28_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Actin-related protein 2/3 complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_35194</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as a component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="function">
        <text evidence="7">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Arp2/3 complex plays a critical role in the control of cell morphogenesis via the modulation of cell polarity development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ARPC5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036901.1">
        <property type="nucleotide sequence ID" value="XM_019182281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX28"/>
    <dbReference type="STRING" id="683960.A0A1E3NX28"/>
    <dbReference type="GeneID" id="30199527"/>
    <dbReference type="OrthoDB" id="195498at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.190:FF:000003">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.190">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006789">
        <property type="entry name" value="ARPC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036743">
        <property type="entry name" value="ARPC5_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12644">
        <property type="entry name" value="ARP2/3 COMPLEX 16 KD SUBUNIT P16-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04699">
        <property type="entry name" value="P16-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039096">
        <property type="entry name" value="p16-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69103">
        <property type="entry name" value="Arp2/3 complex 16 kDa subunit ARPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="151" mass="16808" checksum="CEA7DEA4DD23CE47" modified="2017-01-18" version="1">MEDWRRIDIDALDTENQILAEELKPDVPPVSAEEVQSKISTLRSYISKGSYNDAILFITEDPPYGADESSKAQLLNSVLDVLSAVKQSDIPTIVESLSIEQQNVLVKYLYKGMSVSKGQQQGGILLSWYDKTVDITGLGPVVRYLSDRRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXB4</accession>
    <name>A0A1E3NXB4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_81517</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the p23/wos2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036509.1">
        <property type="nucleotide sequence ID" value="XM_019185246.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXB4"/>
    <dbReference type="STRING" id="683960.A0A1E3NXB4"/>
    <dbReference type="GeneID" id="30202492"/>
    <dbReference type="OrthoDB" id="1564555at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032212">
        <property type="term" value="P:positive regulation of telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06465">
        <property type="entry name" value="p23_hB-ind1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000013">
        <property type="entry name" value="Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045250">
        <property type="entry name" value="p23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22932:SF1">
        <property type="entry name" value="CO-CHAPERONE PROTEIN DAF-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22932">
        <property type="entry name" value="TELOMERASE-BINDING PROTEIN P23 HSP90 CO-CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="3">
        <location>
            <begin position="2"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="118"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="118"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="208"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="223" mass="24541" checksum="C22979E265E84C20" modified="2017-01-18" version="1">MAIPPEVLWAQRSNKDDETKNVLFVTVKISDSKNTKVDLTSTHLNIESDSDETKDHYKLNLEFYKEVDPEQSHHHTSGNSIYFVIRKKEKQEEFWPRITKEKLKYHYIKTDFDKWVDEDEQDEKATEDELAGMGGPPGFGGGANESFNMNDLAALQGLAGGQGGAGGAPDFSQLANLAGGAGGDFDFSKLAAGAGPVDPQTTEADFSSSDEEDQTEEVTEKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXE9</accession>
    <name>A0A1E3NXE9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_65184</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ57836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXE9"/>
    <dbReference type="STRING" id="683960.A0A1E3NXE9"/>
    <dbReference type="GeneID" id="30201116"/>
    <dbReference type="OrthoDB" id="3980255at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244:SF471">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT BETA 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244">
        <property type="entry name" value="KELCH REPEAT DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="692" mass="77723" checksum="668F2E4BA69EF726" modified="2017-01-18" version="1">MSLTPLLAVSYTSNYGETDAGQSHKSMERKLKHYYLSLMRSQSKGLDGINIGNDSSEEETETFKDSISNPYMFIHDQEYDNLEYNKISTPKDYSRNYMKSHKIWTPHANTNGILNILNHQKGFSSEALPPPKVDPDRCSSYSVVDDAKFLNYLHLPKTMGKDQLPTLIYHTSCEVNGNIYLLGGASTFFNQNGVIKDLSNYIVDGIKLPKPINSRLLNNPTVIPNDHLFVISSETNLVSKPEVSGDVPPPLLSMSASKITNRHIFYYGGFEVINQVQYDNSKNKIHIKKSVKLNNSGYVLDVMSLKFRKFELIAHPNMITRYPLTVPRFGHSSCAVNLAKHMKSDENSSASGIAATVFIMGGFRQDPEDPTDFKAIKDLWKVELSITYKGKHDYVQFGESVLATPIPGPIDDASIPCARAFHSAEILDASIVHGHHTFNNKSKVHNPNKVGIARPSSPTVSLISQNSSSSNNKPFNEELLDLMLIVHGGTDGAELFGDVWWFNFDEENWYSISTYFHDFDQNLIPKETVSEVQMKRAGHRCILIDKYFYVIGGGMPSDFDDFDQEKIDQDQFPDISPLVANIKCRLKGSSSKQDQYQRLFILNLQTQTWLMTKYFHGILPLFGTSQSLSYFGNIGATVTQSNSKVFVVGGQLIPHHKYDNHLNESRRVNILNSGLSIFEFPLGSGLKSFGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXM8</accession>
    <name>A0A1E3NXM8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_81522</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036514.1">
        <property type="nucleotide sequence ID" value="XM_019185248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXM8"/>
    <dbReference type="GeneID" id="30202494"/>
    <dbReference type="OrthoDB" id="4096984at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="319" mass="37392" checksum="DE5C28192A159D27" modified="2017-01-18" version="1">MSHQSFKTIQRQVNGLLNQSRSETNCLKAIKLCRGFLKDNPTTSSTFNENSINEIIKYKKPDLEQFLFDKLRLQSQPSNVLRSYLVENPVPQSRFIFDAVKGNTDIELNKTALRQLLYKNKDVETSLSLIKMNRNHFEVKEVKNLQKLGLMYVGGVLSFNGLLEFAIAGHEAIHLLFDIYLFNMFFLTFFLKKGYSDLVTWRPGISMFQKYFHHYDYKMCHKLVSIFDELYTTNVSNYHLTNSILIEQFQKDMVLDREQIEKLIRDEFREMGLMITEGSDEAMFKEYWSTGGDGFEWVEPDRDPVEFLEIAAKKLETEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXU7</accession>
    <name>A0A1E3NXU7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_64177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037236.1">
        <property type="nucleotide sequence ID" value="XM_019183683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXU7"/>
    <dbReference type="GeneID" id="30200929"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="261" mass="29791" checksum="0A37E46B1B86ED47" modified="2017-01-18" version="1">MNENNSSFQKLPELEASNERLTLVVEYQANNREEFVERLAELLEKSETIPHHKKVIKIKQFKGSQIKKTLSWDVEGENLVRRKEITNQDGHLESTAVPENDGEGLEAGVDFAPGMTYSLSLSKGRLLSVTRFEKIFTRSKLDLNEVKTISLIFLIVCQLCVSAILWLNDYQSSGDQLWYFIPSYIAAATSVEILCYYLIIPSEEEKATGKSESAKRLTTLKIVGWSITLIGFVIFIGIRENFFSKVILYIIHLALYLIRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY28</accession>
    <name>A0A1E3NY28_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein PBDC1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_64215</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PBDC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037275.1">
        <property type="nucleotide sequence ID" value="XM_019183689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY28"/>
    <dbReference type="STRING" id="683960.A0A1E3NY28"/>
    <dbReference type="GeneID" id="30200935"/>
    <dbReference type="OrthoDB" id="10248897at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990593">
        <property type="term" value="F:nascent polypeptide-associated complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061770">
        <property type="term" value="F:translation elongation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3560.10:FF:000001">
        <property type="entry name" value="Protein PBDC1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3560.10">
        <property type="entry name" value="yst0336 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023139">
        <property type="entry name" value="PBDC1-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008476">
        <property type="entry name" value="PBDC1_metazoa/fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021148">
        <property type="entry name" value="Polysacc_synth_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13410">
        <property type="entry name" value="PROTEIN PBDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13410:SF9">
        <property type="entry name" value="PROTEIN PBDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04669">
        <property type="entry name" value="PBDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polysaccharide biosynthesis" evidence="5">
        <location>
            <begin position="15"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00061201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00069779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="144" mass="17202" checksum="351AF62B1404C0AF" modified="2017-01-18" version="1">MSTFNAETAENLEDIEKQFAVKAVHQAETYWNLLTKIKGSKLKLTKYDDDIYDLLVEKFPEFKDPEYARSINEDEMKSKTGKERWRLFCEEFKHIEDYNFGTLLRTKSDSEYDQETTIFVVRIQFYAIEIVRNRAGQNDWIYGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYB1</accession>
    <name>A0A1E3NYB1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CoA-transferase family III</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_80312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CoA-transferase III family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037363.1">
        <property type="nucleotide sequence ID" value="XM_019185038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYB1"/>
    <dbReference type="STRING" id="683960.A0A1E3NYB1"/>
    <dbReference type="GeneID" id="30202284"/>
    <dbReference type="OrthoDB" id="5863171at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="entry name" value="Crotonobetainyl-coa:carnitine coa-transferase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="entry name" value="formyl-coa transferase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052985">
        <property type="entry name" value="CoA-trans_III_biosynth/detox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48229:SF1">
        <property type="entry name" value="ALPHA METHYLACYL-COA RACEMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48229">
        <property type="entry name" value="CAIB/BAIF FAMILY ENZYME (AFU_ORTHOLOGUE AFUA_1G05360)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="CoA-transferase family III (CaiB/BaiF)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="471" mass="51293" checksum="8C98258705A16D47" modified="2017-01-18" version="1">MTALQELLAIRGGAPLKADEVEITGSDPIYKTPFKLGGTTAEVLAACGVAANDLWELKTGRRQKVSVNVEAAAGGLDEKNNSLAKNQDGEYSRIEGSESMKHMASLTQPWETSDHKWFLPHTNLKHLEARVLKVLECESTVESLQKAISKRTAQELEDSIAQANACGGIARTEEEWLQHPQGKYLSSVPVVEIAKIIDSPKESLSDKGDMPLSGVRVLDLTRILAGPVCGLTLAELGADVLMVTAEKLPQVNEFVRDTSHGKRSCFLDISKKEDASKLKELVKDCDILIDGYRPDRLAAYGFGPEELVKLRPGLIHVSISCFGSGGPFSSRAGWDQVAQAVTGMCVKQGESIGSNKPQLTPVFACDYVCGRLGAFGAMLALNRRAQDGGSYSIQVSLCQAAMLLQRQGYVDDFLDAPNLISKELFEKYQVIEDNTAYGDLKVLGPVLKLSETPCQWVRKTPKLGQDKPEWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYG1</accession>
    <name>A0A1E3NYG1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_80394</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL36 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037456.1">
        <property type="nucleotide sequence ID" value="XM_019185058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYG1"/>
    <dbReference type="STRING" id="683960.A0A1E3NYG1"/>
    <dbReference type="GeneID" id="30202304"/>
    <dbReference type="OrthoDB" id="9616667at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1760:FF:000003">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1760">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000509">
        <property type="entry name" value="Ribosomal_eL36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038097">
        <property type="entry name" value="Ribosomal_eL36_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10114">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01158">
        <property type="entry name" value="Ribosomal_L36e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01190">
        <property type="entry name" value="RIBOSOMAL_L36E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ58249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="102" mass="11560" checksum="46B54C2ABBCD9516" modified="2017-01-18" version="1">MATKSGIAVGTNKGRKVEQLEKAPKISYRKGALSNRAKFVRSIVREVSGLAPYERRLIELIRNAGEKRARKVAKKRLGTFQRAKAKVEEMNNIIQESRRQGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYP3</accession>
    <name>A0A1E3NYP3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_81150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037029.1">
        <property type="nucleotide sequence ID" value="XM_019185183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYP3"/>
    <dbReference type="GeneID" id="30202429"/>
    <dbReference type="OrthoDB" id="10554396at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="373" mass="42078" checksum="95F4841358A777B7" modified="2017-01-18" version="1">MLVQYGTGPGELVDMDNHSVHEAPSSARAPSFGSADHYDYGVKLDETEKPSSSIKYHMLGIVQLVLKMYSLGVVLLIVTLFELKRTVVSVLNHLALVITSLLSTCLFILVLFHQMILSLRENIHYLLHLEDSSTTSCTKFPSRVKYSELHSLFWSSDKSHKIRISDDFQSFEIIPTNNINLSYAKKRLNSDSTVVADPLAVDVCNEPQGINLSFISSPGISKVQQFNNKYKEKLKKRPHWWKVGTSPISIPEGHASEDREQSQESLDNDEEIHHGSDPGSPLEVMKSSDFENQKFVLASSPLDLNETEYQSHSQQQQSPIKLSPSNSSKFHSKFKVAMESNRQNVIQNYAKSLKTFKNHHISTNLHHSLQDSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYT3</accession>
    <name>A0A1E3NYT3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_34029</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037500.1">
        <property type="nucleotide sequence ID" value="XM_019182142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYT3"/>
    <dbReference type="STRING" id="683960.A0A1E3NYT3"/>
    <dbReference type="GeneID" id="30199388"/>
    <dbReference type="OrthoDB" id="272141at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030437">
        <property type="term" value="P:ascospore formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF4">
        <property type="entry name" value="PROTEIN KINASE MCK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="28"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="363" mass="41363" checksum="8C916F999421481E" modified="2017-01-18" version="1">MSSSYVDTEFVADDVTSNKTNVTKKMLIKEYKKIGEGAFGTVTQAKLLLDGESWLGPFAIKKVPAQTEYKSRELEILRLTNHPNIITLEYFFTHKSPSDGKIYQHLAMECLPETLQFEIRLHYSNKLELPLKHIKLYTYQIARAMLYLHALGICHRDIKPSNILVDPSTGVLKICDFGSAKRLEPRQPSVSYICSRYYRAPELIVGCQYYSTQIDIWGLGCVLAEMILGKAIFQGDDPILQLREICKLLGPPDKRFIYNSNPKYDGPLFSKPLFNGKVSTRFTKILGPVGETGIDLLTKVLVFEPEKRLSPRRILAHEFFDDLRVDSQFVPRGSSTPVELPKLFNFSSFELDVIGELRDRITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYW3</accession>
    <name>A0A1E3NYW3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_80466</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WHI5/NRM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037530.1">
        <property type="nucleotide sequence ID" value="XM_019185069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYW3"/>
    <dbReference type="GeneID" id="30202315"/>
    <dbReference type="OrthoDB" id="5345625at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013734">
        <property type="entry name" value="TF_Nrm1/Whi5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08528">
        <property type="entry name" value="Whi5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="233"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="262" mass="28792" checksum="C1B2AF19A3CBB2F6" modified="2017-01-18" version="1">MSTFERPVLSVVSANALNKKHNSDLGNIKPTLPNLRSIISSKSFSNSNYSTTLSSIKKPTPSSKYTKNAEKLKYRLQLAYYKLRTDQVSVPIGEIIKKQEAKLQKQHHQPKKPIVTPSLTRHISLLAASTPLQSNPITNQKKKVSFKPIELPPTSKFSTNPTKTSSAALSRISEYQRPGNLRSLSLPQLGKSDSAHQHRSLKIEDLVNNTTQARVDVTPVKGRADENGDLIMSSPTKKLHSSTPGSYGAAKSLLQLSLISSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ38</accession>
    <name>A0A1E3NZ38_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_80595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037663.1">
        <property type="nucleotide sequence ID" value="XM_019185089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ38"/>
    <dbReference type="GeneID" id="30202335"/>
    <dbReference type="OrthoDB" id="4019919at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116907">
        <property type="entry name" value="Hook domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="209"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="308" mass="36318" checksum="1DB5D4C54D905502" modified="2017-01-18" version="1">MPLEESNLKLISAVINWLSLFPNSTITSNIFNEIWKPDVVVEILQIILDSSFEVNLVKGLDTVEQYELIFNNVKQYYKQHMISRKKFYKYLDLLKFEELSKGDLLSLNCLCFIFLEIGFHCYKSSVAISIISLLNESDEEELKKFMYDEDDLNCKNLILNQKVNLLELENGKLLQKISSRDKELIELQKNMYLKNQNDVNLNLEFNNGLKIKNDEVLNLKRQLSVLQTKNECLTKENELLNNDLEILRNLKGMLSSSDLNPIYQTIQPKLIESNDDYELLKWEYSLMMEWMSVIQKEKFKRQEKSLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ81</accession>
    <name>A0A1E3NZ81_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phospholipid:diacylglycerol acyltransferase</fullName>
            <ecNumber evidence="9">2.3.1.158</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Triacylglycerol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_34405</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a glycerophospholipid + a 1,2-diacyl-sn-glycerol = a monoacylglycerophospholipid + a triacyl-sn-glycerol</text>
            <dbReference type="Rhea" id="RHEA:14057"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:64615"/>
            <dbReference type="ChEBI" id="CHEBI:136912"/>
            <dbReference type="ChEBI" id="CHEBI:136913"/>
            <dbReference type="EC" id="2.3.1.158"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.158" evidence="9"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037531.1">
        <property type="nucleotide sequence ID" value="XM_019182182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ81"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ81"/>
    <dbReference type="GeneID" id="30199428"/>
    <dbReference type="OrthoDB" id="190846at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046027">
        <property type="term" value="F:phospholipid:diacylglycerol acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006672">
        <property type="term" value="P:ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140042">
        <property type="term" value="P:lipid droplet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055091">
        <property type="term" value="P:phospholipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000160">
        <property type="entry name" value="Phospholipid:diacylglycerol acyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003386">
        <property type="entry name" value="LACT/PDAT_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11440">
        <property type="entry name" value="LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02450">
        <property type="entry name" value="LCAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ58324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="647" mass="73116" checksum="349039358C974BE9" modified="2017-01-18" version="1">MPLKKRSNKTSKHKNLEEKSRLDNDELIEISEDSESTPATNSKNGGSKITSSTSAKPAKKAVKESRRFVFLLGALVGVLIAVYFGAVQHGATDFEKLVNFESLQDYLDDWKDVIPNGVQNALNDFKQSSPGDGKDFSEDFSVGKAMKAENNLTDKYPVIMVPGVISTGIESWSLEGTKDCPSQPHYRKRLWGSMYMLRTMFLDKTCWLKHIMLDEETGLDPPGIKLRAAQGFEAVDFFMAGYWIWNKILQNLSVIGYEPNKMVTAAYDWRLSYLDLEKRDQYFSRMKQQIELRFKISGEKSILIGHSMGSQIVFYFMKWVEAEGEHFGNGGKDWVNKYIAAFIDISGSVLGAPKAVPALISGEMKDTVQLNALAVYGLEKFFSRRERLDMMRTFGGLASMFPKGGDLIWGNLNGALEDVLHNNTDTFGNFIRFEKPIGKFSAKNLTMSDSIDFLLDNSPEWFNRRTKEHYSYGVAKSPKELKENEKFFNKWSNPLEVPLPNAPELKIFCFYGVGNPTERSYIYQEEEDKEISKLNVSILSKSDKQSVFFTDGDGTIPIMTHAMCHKWKTSPNYNPGGSQIKIVEMKHAPDRFDIRGGAKTAEHVDILGSAELNELVLKVASGRDDLIQERVVTNMTEWVASMDFGFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZD9</accession>
    <name>A0A1E3NZD9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Glycosyltransferase family 71 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_34130</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037625.1">
        <property type="nucleotide sequence ID" value="XM_019182150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZD9"/>
    <dbReference type="STRING" id="683960.A0A1E3NZD9"/>
    <dbReference type="GeneID" id="30199396"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000033">
        <property type="term" value="F:alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392:SF1">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="567"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="620" mass="72872" checksum="050C6D25717F5F98" modified="2017-01-18" version="1">MRNKIQEYKKNNEDKEPSDGKKAEFELDFQKMVEVTLGAEQNMIDAVTHLRVFGKCYLNDNGVSSSVLSNWFSEKEAKKQDEEKIDMCYDIEQRLFPWLSRELPVFKRWIDKFNARLLSKRYARSNCFLNTWRSMLNGKGIVLSAADKYESDLVGLLRVLRALNNKLPIQIVHKGDLSEKVQDNLVKVARADDVQVPKYLYDQIKDFTPQNFPKQEIWFVNAKRCIRNEYRGNFNMYANKLIAYLFNSFDDILLMDTDSIPLVKPIELFKSGPYQRTQTIFFKDRFNVEKVSKHDSEYFKRLMPSKIDESLFTIPKTTNHTLENRYIKNQFRHVMEAGMVGIKRSTHFIGILTSIQLNFWTATNSRIWGDKELFWLGQSISGNENYEFNKYDVVAVGELTPMEDRPRNTIAHELCSTHPGHLSGDDDFTLLWINSGMRYCKVDTWEGDMDQERIKKKFKSVKEVETYYKNPVKINAALIPPGGDRSKPNGKDEPAFGWIMTPPCKGYLWCAYDILGGSTDPKDRGAYVEYDNVTEYRNEYLGKLWLNQDLVDLSKYEVQIKVDEEKERVKKVKEDNEKKLREEKEKKEKEERERREKEEKEKKEKEQQEKQAQDGNDHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZI4</accession>
    <name>A0A1E3NZI4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_64650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037722.1">
        <property type="nucleotide sequence ID" value="XM_019183780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZI4"/>
    <dbReference type="GeneID" id="30201026"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="82" mass="8684" checksum="9C05532E13E8243B" modified="2017-01-18" version="1">MSGSKSSNQGMQSQMASNNASNRAMHSQMESNKGSNRTSNRGMNSQMESNNSANRSMHSQMASNNASNRGMHEAVSGSKGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZJ1</accession>
    <name>A0A1E3NZJ1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Inosine triphosphate pyrophosphatase</fullName>
            <shortName evidence="12">ITPase</shortName>
            <shortName evidence="12">Inosine triphosphatase</shortName>
            <ecNumber evidence="12">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="12">NTPase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">XTP/dITP diphosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">HAM1</name>
        <name evidence="13" type="ORF">WICANDRAFT_32318</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triphosphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ITP + H2O = IMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:29400"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(6)-hydroxy-dATP + H2O = N(6)-hydroxy-dAMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:83971"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:233529"/>
            <dbReference type="ChEBI" id="CHEBI:233530"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:83972"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>XTP + H2O = XMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:28343"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="12">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="12">Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="12"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037833.1">
        <property type="nucleotide sequence ID" value="XM_019181943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZJ1"/>
    <dbReference type="STRING" id="683960.A0A1E3NZJ1"/>
    <dbReference type="GeneID" id="30199189"/>
    <dbReference type="OrthoDB" id="6288734at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009204">
        <property type="term" value="P:deoxyribonucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.950.10:FF:000003">
        <property type="entry name" value="Inosine triphosphate pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03148">
        <property type="entry name" value="HAM1_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027502">
        <property type="entry name" value="ITPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="RdgB/HAM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067:SF9">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE/HAM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="65"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="175"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00093255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ58626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="191" mass="21156" checksum="9EC24ACA25CEB2BD" modified="2017-01-18" version="1">MSSPAITFVTGNAKKLKEFQAIIGDDSIRNQSVDLEEVQGSIKDISTKKAIAAAEVIKGPVLVEDTCLVFNALSKPELELPGPYIKWFVESVGVENLPRLLAGFEDKSAQTVCTFAFTEGPGKEVKLFQGITEGKIVEPRYNGEEVFGFDPIFEPTGFSQTFGEMDKSLKNTISHRYRALEKVKEFLKTRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZU3</accession>
    <name>A0A1E3NZU3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Adenylate kinase isoenzyme 6 homolog</fullName>
            <shortName evidence="10">AK6</shortName>
            <ecNumber evidence="10">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Dual activity adenylate kinase/ATPase</fullName>
            <shortName evidence="10">AK/ATPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_32994</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has also ATPase activity. Involved in the late cytoplasmic maturation steps of the 40S ribosomal particles, specifically 18S rRNA maturation. While NMP activity is not required for ribosome maturation, ATPase activity is. Associates transiently with small ribosomal subunit protein uS11. ATP hydrolysis breaks the interaction with uS11. May temporarily remove uS11 from the ribosome to enable a conformational change of the ribosomal RNA that is needed for the final maturation step of the small ribosomal subunit. Its NMP activity may have a role in nuclear energy homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with small ribosomal subunit protein uS11. Not a structural component of 43S pre-ribosomes, but transiently interacts with them by binding to uS11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the adenylate kinase family. AK6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="10"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037832.1">
        <property type="nucleotide sequence ID" value="XM_019182023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZU3"/>
    <dbReference type="STRING" id="683960.A0A1E3NZU3"/>
    <dbReference type="GeneID" id="30199269"/>
    <dbReference type="OrthoDB" id="10251185at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000372">
        <property type="entry name" value="Adenylate kinase isoenzyme 6 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00039">
        <property type="entry name" value="Adenylate_kinase_AK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020618">
        <property type="entry name" value="Adenyl_kinase_AK6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595:SF0">
        <property type="entry name" value="ADENYLATE KINASE ISOENZYME 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595">
        <property type="entry name" value="POS9-ACTIVATING FACTOR FAP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 10" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="NMPbind" evidence="10">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="10">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="184" mass="20840" checksum="08C6C9B9BD179228" modified="2017-01-18" version="1">MSYRPLPNIILTGTPGCGKTSHAELISQEIKGFKHVNITDFAKENDAYDGFDKERNSHIVDEDKLLDALEPLLEKGGILIDWHANDLFPERLIDLVVVLRTENGILYDRLSKRDYSQSKIDENLDCEIMEVILQEAREAYAPEIIVELQSNTTEDIDSNVARVISWIDTWKVNNPDGASNELAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZV0</accession>
    <name>A0A1E3NZV0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aldehyde dehydrogenase 5, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_80365</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Minor mitochondrial aldehyde dehydrogenase isoform. Plays a role in regulation or biosynthesis of electron transport chain components. Involved in the biosynthesis of acetate during anaerobic growth on glucose.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037419.1">
        <property type="nucleotide sequence ID" value="XM_019185054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZV0"/>
    <dbReference type="STRING" id="683960.A0A1E3NZV0"/>
    <dbReference type="GeneID" id="30202300"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004030">
        <property type="term" value="F:aldehyde dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019413">
        <property type="term" value="P:acetate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="Aldehyde dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mitochondrial aldehyde dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="7">
        <location>
            <begin position="45"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00053679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00069627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ58212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="506" mass="55599" checksum="51A6D74A0189C0D7" modified="2017-01-18" version="1">MSSLILRRFQSTFSRQLAKTIPITLPNGLKYNQPTGLFINNKFYETNESKIKVINPSTEQDIVSVSSAIKTDVDFAVDSAELAFQHWSKTDPLYRAKLLNNLALKIEENFEIMASIESMDNGKTLALAKGDVQLVIDYFRSTAGYCDKLDGRSISENGYMNFTIREPLGVVGQIIPWNFPLLMLSWKIAPALATGNTVVLKPASDTPLNALFFANLVKEAGIPDGVVNILPGSGRTCGEAILQHPKIRKIAFTGSTSIGRDVMVAAAKSNLKKITLELGGKSPNIVLEDCDQDKTLQILVDGIFKNAGQICSSGSRIYIQDSIYDEMLIKFKNYIENEIKVGDPFNSENYQGAITNKSQFNTILSYIDIGLKSGHAKLLTGGERIGDVGYFIKPTVFYDVEQDSQINKEEIFGPVVTISKFSSIDQVVTMANDSEFGLGAGIQTTNLSKALQIAQLIKSGTVWINTYNDFSPNVPFGGVKQSGFGREMGQEALDNYTQVKAVRIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P030</accession>
    <name>A0A1E3P030_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NOT2/NOT3/NOT5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_84400</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037831.1">
        <property type="nucleotide sequence ID" value="XM_019185703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P030"/>
    <dbReference type="STRING" id="683960.A0A1E3P030"/>
    <dbReference type="GeneID" id="30202949"/>
    <dbReference type="OrthoDB" id="25391at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="4">
        <location>
            <begin position="47"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ58624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="171" mass="20158" checksum="60DB17B52DC76054" modified="2017-01-18" version="1">MDRYGLNGLLGLLRAENPDQNTVAIGSDLNILGLDMSEKSIDVKLSKTFPSPWIETSRSEVEPQFTVPEYFKVDSLSKVEDKLTLFTDETLFYIFYTRTKDVLQELSARELMNRNWRYHKELQVWLTKDSNTEPIQQSPQSERGLYIFFDPHNWEKVRKEFILYYQAIMST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0B8</accession>
    <name>A0A1E3P0B8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_105752</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 21 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038103.1">
        <property type="nucleotide sequence ID" value="XM_019180453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0B8"/>
    <dbReference type="STRING" id="683960.A0A1E3P0B8"/>
    <dbReference type="GeneID" id="30197699"/>
    <dbReference type="OrthoDB" id="526653at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.10">
        <property type="entry name" value="Mediator complex, subunit Med21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037212">
        <property type="entry name" value="Med7/Med21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021384">
        <property type="entry name" value="Mediator_Med21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13381:SF0">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13381">
        <property type="entry name" value="RNA POLYMERASE II HOLOENZYME COMPONENT SRB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11221">
        <property type="entry name" value="Med21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140718">
        <property type="entry name" value="Mediator hinge subcomplex-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0010">Activator</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="139" mass="15779" checksum="F3A1E89D34342522" modified="2017-01-18" version="1">MADRLTQLQICLDQLVEQFCATLNYVDKNHDFVPAQDEEKMADAQATVADPKEFQSTMNELSTDIILKTRQILALITSLPGAGVSPRDQISRIQELQNELVSIEQTKVEKIRQKDELLEWCNGLISDFSKDLIDSRRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P165</accession>
    <name>A0A1E3P165_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_17576</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038280.1">
        <property type="nucleotide sequence ID" value="XM_019180750.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P165"/>
    <dbReference type="STRING" id="683960.A0A1E3P165"/>
    <dbReference type="GeneID" id="30197996"/>
    <dbReference type="OrthoDB" id="2020758at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02662">
        <property type="entry name" value="Peptidase_C19F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF888">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="25"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="397"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="401"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="420"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="511" mass="57951" checksum="97F36AD13013C553" modified="2017-01-18" version="1" fragment="single">RRFRYGSNQNTKQQKKLMKKGGNIGGLINDGNTCFMNSVLQSLASSNSLISFLNDETIEGDIKEKDLKFTKSFKTLLENLNNKHFNKNHDYKTNPMLKTMTKTPNKNFLMGYNQEDAQEFFQSILSELESDFKKIHSSNTTTNKTEPVLTSSLPSDTTHGIDNLGYIGPVYIPSSQIDPNLQDSQLKSTRFNLLTPVDGLSAERIGCLQCGEMGGIRYSVISGLSLNLPPDNYNALKLTDLLTKWIEPEIIEGVDCNRCALNSVLQYQQDQLSTLEAKEQTSSTEKLIELLKLRVGEIERELEKPIIDDEIFKKLHTKNMMKKCSKSKQILISRPPPLLAIHINRSVFDPMTYRVRKNNARVVFPLKLDLDPFVAEPNDINTDARLPLSKKVKVQEPIVQSESSSTSSLETPDSSNNEKEEGEEKLEQDSDSDSDEQEIPLSIEKQDISGPLVYALRSVIVHYGTHNYGHYIAFRKYRGVWWRTSDETIDIVDEDEVLSAPGVFMLFYELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1B8</accession>
    <name>A0A1E3P1B8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Malate synthase</fullName>
            <ecNumber evidence="3 11">2.3.3.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_63630</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 11">
            <text>glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="11">Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with PEX9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the malate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.9" evidence="3 11"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038340.1">
        <property type="nucleotide sequence ID" value="XM_019183584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1B8"/>
    <dbReference type="STRING" id="683960.A0A1E3P1B8"/>
    <dbReference type="GeneID" id="30200830"/>
    <dbReference type="OrthoDB" id="186072at2759"/>
    <dbReference type="UniPathway" id="UPA00703">
        <property type="reaction ID" value="UER00720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004474">
        <property type="term" value="F:malate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00727">
        <property type="entry name" value="malate_synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1220.12:FF:000001">
        <property type="entry name" value="Malate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.360:FF:000001">
        <property type="entry name" value="Malate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.360">
        <property type="entry name" value="Malate synthase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1220.12">
        <property type="entry name" value="Malate synthase, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044856">
        <property type="entry name" value="Malate_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011076">
        <property type="entry name" value="Malate_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006252">
        <property type="entry name" value="Malate_synthA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019830">
        <property type="entry name" value="Malate_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001465">
        <property type="entry name" value="Malate_synthase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048355">
        <property type="entry name" value="MS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048356">
        <property type="entry name" value="MS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046363">
        <property type="entry name" value="MS_N_TIM-barrel_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01344">
        <property type="entry name" value="malate_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902">
        <property type="entry name" value="MALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902:SF1">
        <property type="entry name" value="MALATE SYNTHASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20659">
        <property type="entry name" value="MS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20656">
        <property type="entry name" value="MS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01274">
        <property type="entry name" value="MS_TIM-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001363">
        <property type="entry name" value="Malate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51645">
        <property type="entry name" value="Malate synthase G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00510">
        <property type="entry name" value="MALATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Malate synthase N-terminal" evidence="13">
        <location>
            <begin position="22"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Malate synthase TIM barrel" evidence="12">
        <location>
            <begin position="173"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Malate synthase C-terminal" evidence="14">
        <location>
            <begin position="423"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001363-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF20659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ59133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="553" mass="62813" checksum="18A0F0769CC3A832" modified="2017-01-18" version="1">MVRKTLDNVQLLGKLDSSPKYPSNTTPQHLLTKSALEFITLLHRTFNPTRLQLLSNREKVQQHLDNGGELTFLQETQQIRNDPTWTGPPLAPGLIARNTEITGPPSSRSMLVNALNSDVKTYMTDLEDSASPTWENVIYGQLNLYDAIRGQVDFTNEQTGKTYKVNKEKTTPTLILRPRGWHMVDNHILVDNEPISASLFDFGLYFFHNCHELIKLGHGPYFYLPKMEHHLEARLWNDVFNVAQDYMGITRGTIRATVLIETLPAAFQMEEIIWELRQHSSGLNCGRWDYIFSTIKRLRNDPSHVLPDRDQVTMTSPFMNAYVEQLIKICHSRKVHAMGGMAAHIPIKNDIQANNAAMAKVQNDKIRELTKGHDGSWVAHPALAPICNEVFQEMGTPNQIHFIPDYKTNAEDLTNTKIDNARITENGIRINLYIGLSYMEAWLRGSGCVPINNLMEDAATAEVSRSQLYQWVKHGVVLDDTGEKVTPELTTRLLKEETAKLLKNAPVDNKFEIAAGYFKPEIRGERFSEFLTTLLYDEISTIGEPVDLSTLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1D1</accession>
    <name>A0A1E3P1D1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Plasma membrane proteolipid 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_79780</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038468.1">
        <property type="nucleotide sequence ID" value="XM_019184949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1D1"/>
    <dbReference type="GeneID" id="30202195"/>
    <dbReference type="OrthoDB" id="10586373at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="96"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14214" checksum="538D891A2AA23F60" modified="2017-01-18" version="1">MYFINNYTLIILALIFPPIPVIITKGIRKDHSLLLNLLWHFIGYIPAVIHSIFVIAQCEYGGFVSKPSDWKYIKLNSPSYPLMEMGRAFVAIERTESGESDDSVDSTSCVQLDNSPMSLKDDDTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1H2</accession>
    <name>A0A1E3P1H2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Isocitrate lyase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_33016</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Can also use Mn(2+) ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038462.1">
        <property type="nucleotide sequence ID" value="XM_019182024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1H2"/>
    <dbReference type="STRING" id="683960.A0A1E3P1H2"/>
    <dbReference type="GeneID" id="30199270"/>
    <dbReference type="OrthoDB" id="4078635at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004451">
        <property type="term" value="F:isocitrate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046421">
        <property type="term" value="F:methylisocitrate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019629">
        <property type="term" value="P:propionate catabolic process, 2-methylcitrate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006254">
        <property type="entry name" value="Isocitrate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018523">
        <property type="entry name" value="Isocitrate_lyase_ph_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01346">
        <property type="entry name" value="isocit_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21631">
        <property type="entry name" value="ISOCITRATE LYASE/MALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21631:SF13">
        <property type="entry name" value="MITOCHONDRIAL 2-METHYLISOCITRATE LYASE ICL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00463">
        <property type="entry name" value="ICL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001362">
        <property type="entry name" value="Isocit_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00161">
        <property type="entry name" value="ISOCITRATE_LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="111"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="219"/>
            <end position="220"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="432"/>
            <end position="436"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR001362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="546" mass="62091" checksum="89CDE91EDEC59910" modified="2017-01-18" version="1">MLGKRFQSTISRFPYPSRSEQFKYYEEQIESIKQWWSSPRFKGIKRTYTAELVAGHRGSFNIQYPSSIQAQKLFNLLNTRFDEGKPVHTIGSIDPVQITQSAKNQEIAYVSGWACSSVLTTTNEVSPDFGDYPYDTVPNQVERLFKAQLLHDKKSYSDWLQNPKRDEVDRIDYLRPIIADADTGHGGTGSVMKLAKLFAERGAAAIHIEDQLHGGKKCGHLSGKVLVPTSTQISRLIATRLQWDIMNTENLVIARTDSETGNLLSSTVDPRDHEFILGVVDPVKPLSEVLYEAEAQGANSEHINSVEQEWVENHKLYTFNEAVESKLKDLKKMDLYANFLQQSQNKSLYDSKLIFKRLSGGEEIFFDWEAPKTREGYYLVKASIDVAVKRVIEYAPYADLLWLETKTPDLKYAQEFAKRIHQVYPDKKLVYNLSPSFNWSAHGFTEHDLQQFIWDLAKSGFVLQLVSLAGLHTNAVSNYELSKNFKDQGMKAYVELVQRKEKEIGCDVLTHQKWSGANYYDDLISAVQSGSSQTSSVGKDSTEHSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1W2</accession>
    <name>A0A1E3P1W2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_79913</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038602.1">
        <property type="nucleotide sequence ID" value="XM_019184974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1W2"/>
    <dbReference type="GeneID" id="30202220"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF284">
        <property type="entry name" value="EG:103B4.3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="405"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="469" mass="52259" checksum="E658B96AA8BA32C8" modified="2017-01-18" version="1">MNQPYTPIKRKSTSDLINNQIQLESTPPNTTITDEQIEFPEGGIQSYVPLMYESTLSISPVIATLTNLSIFAKLLNTLLETSYATSNSWIMTWFLFMFFNGMTLFGDYSDKFTTILPTIIGSILVLISTSLIPTTSSFLGMLLIFGILGLGSSCQYLSSLITISHFFHKRRGYALGVCLSTGGVATSWFVNILLTSVLDDVDELNKIIYLFAGLWGCQCFLTIMYSLNRITDEHDFRLRSPILKELDKKRDVLGRSWCPYIGTDLRLVQDFKFRNNSIAIGIGFGILFIVYSYLDIFINASNATPTEDTYFITQLITCFAIFSSVFIGVLSDSIGVFNVQIGSFLHLSIINLILLPFTNHHLYILYAYLGVLTISAFSIVITSFLSISALSTNTLELGSRLSNSFGIASIPALIISRIAGSILGYQIEEFSAAKFGVLITLFSLFGIYFVNNVNEHSIILFQQKLSRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1Z6</accession>
    <name>A0A1E3P1Z6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_56280</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038713.1">
        <property type="nucleotide sequence ID" value="XM_019182778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1Z6"/>
    <dbReference type="GeneID" id="30200024"/>
    <dbReference type="OrthoDB" id="3979586at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032218">
        <property type="term" value="P:riboflavin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF177">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER MCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="38"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="5">
        <location>
            <begin position="38"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="430" mass="46858" checksum="1F7A0BDFC7E0B164" modified="2017-01-18" version="1">MSNSNDIELHETHLTTPDTSQETLEDDDFPEGGKDANLVVLGSLLGLLITFGIMNSVGVIQAYVATHQLETASTTSVGWVFSIYFFVSFIGSILTGPYFDAKGAKSSMIIGSLLMVGGLFATANCNTIYQFLLAFGFVFGMGSSFVMTATLGSVSHWFNKRRALALGGCTIGGSLGGVIWPLMFRSLFPMIGFQWSLRLMGLISLICLSISTYLLKVRLPAVKSENFIKDSFVMMDLIHEPRFLTLSAAILLGEFSLILVFTYMPSYTMYHGYSESDAFLVTITCNCVGLIGRSLPSYAGDHFGRFNVMCISVVACTILIFIIWLPFGSNLKIMYTFAAFYGLFNSTFQALTPVCCGQISKTKDFGKRYGTVYFFVSSGNLIALPVGGALIGNGKGYNNLIILAGVTEALSAILWIISRVISTGWKLNKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P282</accession>
    <name>A0A1E3P282_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">dihydrolipoyllysine-residue succinyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">2-oxoglutarate dehydrogenase complex component E2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_63508</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.61" evidence="5"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038214.1">
        <property type="nucleotide sequence ID" value="XM_019183563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P282"/>
    <dbReference type="STRING" id="683960.A0A1E3P282"/>
    <dbReference type="GeneID" id="30200809"/>
    <dbReference type="OrthoDB" id="5391403at2759"/>
    <dbReference type="UniPathway" id="UPA00868">
        <property type="reaction ID" value="UER00840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004149">
        <property type="term" value="F:dihydrolipoyllysine-residue succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033512">
        <property type="term" value="P:L-lysine catabolic process to acetyl-CoA via saccharopine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000006">
        <property type="entry name" value="Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050537">
        <property type="entry name" value="2-oxoacid_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006255">
        <property type="entry name" value="SucB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01347">
        <property type="entry name" value="sucB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416:SF5">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="14">
        <location>
            <begin position="76"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="151"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="165"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="212"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ59007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="456" mass="50272" checksum="6B255D09CF542816" modified="2017-01-18" version="1">MLSSRNLRTISKRLPSNATTLVSSQSKNTIKLGSQLRNLSYKSALTTSSASSSLLRNTVRSNVKFQPTLAFVRLNSQVVKVPDMAESLTEGTLREFNKQIGDFVEQDETIATIETDKIDVEVNAPFSGTIKEFLAQPEDTVEVGQDLLKIEPGEAPAGGAGAAAPKDEAPKEEAKKDDEAPKEQPKKEEAPKKEEPKKESKPAPKKEESKPTQQSQETTSFTKFPRTESRVKMNRMRTRIAERLKESQNTAASLTTFNEVDMSSVMDLRKLYKDEIIKKNDIKFGFMGLFSKAATLAMKDLPSVNGAIEGDQLVYRDYVDISIAVATPKGLVTPVVRNAESLSVLEIEQEIVNLGKKARDGKLTLEDMAGGTFTISNGGVFGSLYGTPIINLPQTAVLGLHGVKERPVVVNGQIVSRPMMYLALTYDHRMLDGREAVTFLKTVKELIEDPRKMLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2F8</accession>
    <name>A0A1E3P2F8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_79615</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038289.1">
        <property type="nucleotide sequence ID" value="XM_019184921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2F8"/>
    <dbReference type="GeneID" id="30202167"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="175" mass="20468" checksum="013D9E7CCF48CAE8" modified="2017-01-18" version="1">MDRIRLNIQTTNRQAQPQTTSNNLQQHTNFIINNFNSNNNHHVQYTAARNNIKSVNNSFYLSYFKRLGIHDGSIQLIGSSIGDYTKLLIPLMQNLSKSSQPFLNFDLIEQFPNQDNCWMPKSNCCYVSHEFLMNVLLLSNVVEKDQRQKICHPMKSDRPDHTYYWELTPSQAKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2H0</accession>
    <name>A0A1E3P2H0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_31509</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. TatD-type hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038299.1">
        <property type="nucleotide sequence ID" value="XM_019181842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2H0"/>
    <dbReference type="STRING" id="683960.A0A1E3P2H0"/>
    <dbReference type="GeneID" id="30199088"/>
    <dbReference type="OrthoDB" id="6079689at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050891">
        <property type="entry name" value="TatD-type_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10060:SF15">
        <property type="entry name" value="DEOXYRIBONUCLEASE TATDN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10060">
        <property type="entry name" value="TATD FAMILY DEOXYRIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01090">
        <property type="entry name" value="TATD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01091">
        <property type="entry name" value="TATD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ59092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="373" mass="42491" checksum="7FA4F5AB5947C490" modified="2017-01-18" version="1">MSDSKPQAAAGFHHLIKYFDIGVNFTDPMFHGSYHGKNHHQSDIEDVILRAKTFNVRRMLLTGSSLEESRTTLEIASSYPDYLYSTVGVHPCSVNEINETNEVEYLQNLKELAISGKKAGIVKAFGEIGLDYDRLHYTPKEHQLKYFQKQLDIAVEVGLPLFLHMRAANDDFIQTIKPYLHQLKNGVVHSFTGTLEELDALLELGFYIGINGCSLKTEENLKVVEKIPLEKLMIETDAPWCEIRKTHAGYKYLTSYPNETYPQVLTKQSSEISLNSIASSKKAPQIKLDPLLPFPVLKKEQYDRYDPTIRTLIGEKASPLIKSRNEPVHIGQVVEIIAKVKNESVETVVKHAWENTLKLFKVEDVDDEEFFTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2P0</accession>
    <name>A0A1E3P2P0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RNA polymerase Rpb6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_91135</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal Rpo6/eukaryotic RPB6 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038727.1">
        <property type="nucleotide sequence ID" value="XM_019186279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2P0"/>
    <dbReference type="STRING" id="683960.A0A1E3P2P0"/>
    <dbReference type="GeneID" id="30203525"/>
    <dbReference type="OrthoDB" id="259769at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020708">
        <property type="entry name" value="DNA-dir_RNA_polK_14-18kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028363">
        <property type="entry name" value="RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006111">
        <property type="entry name" value="Rpo6/Rpb6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002208">
        <property type="entry name" value="PRK01099.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227:SF5">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500154">
        <property type="entry name" value="RPB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000778">
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    </dbReference>
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        <property type="entry name" value="RPB6/omega subunit-like"/>
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    </dbReference>
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        <property type="entry name" value="RNA_POL_K_14KD"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="73" mass="8301" checksum="46176D10E2800E5D" modified="2017-01-18" version="1">MTKYERARILGTRALQISMNAPVLVDLEGETDPLQIAIKELAQKKIPLVIRRYLPDGSYEDWGCDELIVDIDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2Q9</accession>
    <name>A0A1E3P2Q9_WICAA</name>
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        <recommendedName>
            <fullName evidence="3">DUF4110 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_83829</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ59766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019185606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2Q9"/>
    <dbReference type="STRING" id="683960.A0A1E3P2Q9"/>
    <dbReference type="GeneID" id="30202852"/>
    <dbReference type="OrthoDB" id="4447at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025183">
        <property type="entry name" value="DUF4110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052588">
        <property type="entry name" value="Kelch_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46063">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46063:SF1">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13422">
        <property type="entry name" value="DUF4110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4110" evidence="3">
        <location>
            <begin position="546"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="410"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="493"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="363"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="415"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="496"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="528"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="634" mass="73238" checksum="BDC5BEEBDEF9165F" modified="2017-01-18" version="1">MAKKNSKKDKEAKKARAEEKNKKNLSKAEGKNKKKAKKTGEVDEDDVDIDEILANYKKEQEAFEAINVETVTRPSKRINVSMVANPNHGKKELFIFGGENTVDNISAFYNELYTYAPDSDQWRKITSKNSPMHRSSAAIAAHPSGVVLLHGGEFSSPKQNTFYHYSDTWLLDATTKEWTKIEQKNGPSARSGHRITVWKNFFIMYGGFRDLGSSTSYLNDLWVFDITNYKWKQIEFPPNHPTPDARSGHSLIPSADGAVLWGGYCKVKAGKGLQKGKILTDCWMLKMKSDLGAIRWERRKKQGFQPSPRVGCSMVHHKGRGVLFGGVYDYEETEESLDSSFFNDLFTYQVENNRWYSLSLRAQKKKQQKLNKKQNRDEDLEELLNSILAKAKLEDDEEDNEDDDEIEAELKKFDDESDSDEDEQGERNEYPVVNTLPHPRFNAATAVVDDSLFIFGGVWELGERDYSIDSLYSIDLAKLDGMKVYWENLKDVENAKEEEEEDEDDFDEEDDEDEDDEGETEDKKLVAEEDEEDEEDIIEDEPEIPDLRPWLPHPKAFESLRAFYVRTGADFLQWAISNNRDARGKHLKKKSFDLCQDRWWERREQVRIEEDRLEELGGIGEVIEKDTTKTTKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2U1</accession>
    <name>A0A1E3P2U1_WICAA</name>
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        <recommendedName>
            <fullName evidence="2">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_91429</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038870.1">
        <property type="nucleotide sequence ID" value="XM_019186295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2U1"/>
    <dbReference type="STRING" id="683960.A0A1E3P2U1"/>
    <dbReference type="GeneID" id="30203541"/>
    <dbReference type="OrthoDB" id="4822at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071008">
        <property type="term" value="C:U2-type post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045211">
        <property type="entry name" value="TFP11/STIP/Ntr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23329:SF1">
        <property type="entry name" value="TUFTELIN-INTERACTING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23329">
        <property type="entry name" value="TUFTELIN-INTERACTING PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="149"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="205" mass="22849" checksum="0793B8E99A718DD2" modified="2017-01-18" version="1" fragment="single">MSDNEIINEGLQRKRKLNLLDFIKNDGHEQLEQENPNKFEPMNFQRPTTGLGGPKPRDDYDDYEGGEDVEMEDYDSDIRPSFGVGKNHNMLFTQSTPMMFQKASDTKDEDIIPQEQPKEEVKPKLSYSDVEALENKPTSVTKNTFKGKKYGIGAALLKKMGYIEGQGLGKHGQGITQPIEQDVRKVGVGIGGGVVKSTKKAKGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2U9</accession>
    <name>A0A1E3P2U9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_62109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycotina</taxon>
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            <taxon>Phaffomycetales</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2U9"/>
    <dbReference type="GeneID" id="30200555"/>
    <dbReference type="OrthoDB" id="4035860at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="218"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="250" mass="27510" checksum="CA97DB0D1569BF78" modified="2017-01-18" version="1">MSDQTDAGHYPDTSKGIKGRFSWVKRLIQSRSNSSFAMANHRRVSSTNKHQVKINHERHRDENQVPTPVSSNPINSSHSRGNDRTVITDRSNGTPVTSQNRNLVTRFDLSPETDNSFDVDQSLNQSMKTSSKTPSIAHESINTRNDNISTLPVISMSTVSVRSSTFSENSQPSTSGTVLSDHPTLYTNASTVGIPPASIMDRRERLHHPVSLNNYHLTGHNNSSHTINTSTSPTSAIPNEYGTDISSLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P399</accession>
    <name>A0A1E3P399_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flavin reductase like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_100125</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the flavoredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038937.1">
        <property type="nucleotide sequence ID" value="XM_019180287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P399"/>
    <dbReference type="STRING" id="683960.A0A1E3P399"/>
    <dbReference type="GeneID" id="30197533"/>
    <dbReference type="OrthoDB" id="10250990at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33798:SF5">
        <property type="entry name" value="FLAVIN REDUCTASE LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33798">
        <property type="entry name" value="FLAVOPROTEIN OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00903">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin reductase like" evidence="5">
        <location>
            <begin position="48"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="264" mass="29709" checksum="C1B8E4D23D2AE4B5" modified="2017-01-18" version="1">MTKLEHQYNKEWSVGQGSTSDDWKTYKKLELDIQDPSELETNYRFFISGVVPRPVAFVSTLDKEGNQNLAPYSFFNIVSSNPPVFVFGVSKNAARDDGTKDTLTNILDTGELTINFISDWFVDAANYTSIPSPKGIDEFKLAGLTPSPSKKVKPPHVAESAFSIEAKLLTTHEWHTKSNPDQKSGTLIIVEGVHSWVREDILNKERKTIDISKYRPVGRLGAINYTTITDNGYPITRPHMNKSSSEIKEGDWIDELDRLAQIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3D5</accession>
    <name>A0A1E3P3D5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_31345</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038977.1">
        <property type="nucleotide sequence ID" value="XM_019181821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3D5"/>
    <dbReference type="STRING" id="683960.A0A1E3P3D5"/>
    <dbReference type="GeneID" id="30199067"/>
    <dbReference type="OrthoDB" id="10266508at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097308">
        <property type="term" value="P:cellular response to farnesol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036180">
        <property type="term" value="P:filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900445">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000010">
        <property type="entry name" value="Sensor histidine kinase RcsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000002">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1530:FF:000001">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1530:FF:000002">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1530">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18947">
        <property type="entry name" value="HAMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="52"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="145"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="237"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="329"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="421"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="12">
        <location>
            <begin position="495"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="13">
        <location>
            <begin position="873"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1017"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1024"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="10">
        <location>
            <position position="922"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ59770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1140" mass="125576" checksum="4E191D83DCA461DC" modified="2017-01-18" version="1">MATIDIISTLKDPLAFQNAVSTKQDIEATRLQILQFLGDKELEIKHLEKSKKSFQKASMEIGNVLIAVAMGDLTKQVEINDEPDPEILKVKMTINTMVDQLHTFASEVTKIATDVAHGEFGGQVKNQGTVGIWRTLSDNLNVMALNLTNQVREIAEVVRGVAGGDLSRKINVHAQGEILELQEIINTMVDQLKTFAFEVTRVSRETGVEGRLGGQAVVVGAEGIWKELTDNVNAMATNLTNQVRNIANVTTAVAKGDLSKKVTADCKGEILDLKSTINEMVDSLQTFALEVTSLATDVGIKGILGGQAVVNGVEGAWKDLTDNVNAMATNLTNQVRSIAAVTTAVAHGDLSQKIDVKAQGEILELKDTINKMVDSLQVFVSEVTRVAKDVGIDGKLGVQAQVSDVDGLWKEIITNVNVMAANLTSQVRAFAQITAAATDGDFTKFITVEASGEMDTLKTKINQMVFNLRESIKRNTAAREAAELANRAKSEFLGIFSHELRTPFNGILNYTQLTLDTELTQYQREMLSTVHNLANSLLTIIDDILDISKIEANRMTIEQVEFTLRGTVFGALKTLAVKSIEKPLDLIYQVDASFPDYLIGDSFRLRQVILNLVGNAIKFTSKGHVSLSVKKATTSLQQQITQTAPQDDVLVLEFCVSDTGIGIKKDKLDLIFENFTQADGSTTRKFGGTGLGLSISKRLIHLMGGEIWVTSAYGEGSRFYFTIAVRPVPDQVFTKQSDALLTFNLHQILFISTQHSAQETNKIEEDLKGLNLRPTVVHTIDEANLEEPCKYDVIIIDSIDTGNKLRLLPEIKYIPFVLLHHSIPRLNMRVCLDLGIQSYGNTPCSIMDLADYLKPALESRIVPSDDDAPQSYNILLAEDNLVNQKLAVRILEKYGHEVHVAQNGLEAFEEVKQNRYDVVLMDVQMPIMGGFEATEKIRKWEKEMNPIDPLSVRVPIIALTAHAMLGDREKCMQAQMDEYISKPLKPNLLIQTIAKTIHHVNKLKEIASMNPTNDQLANEGLLNFPSNGNNSSTNLAETSPSNKRSQSNQELGKVINNINSTTTKDNLATALSPALKSLERPLLSINNRSVTDSALSTLSSMKNSPVESEFLKRSIDEQVIDDINDNYDDKEDEDGDLEVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3E3</accession>
    <name>A0A1E3P3E3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">diacetyl reductase [(S)-acetoin forming]</fullName>
            <ecNumber evidence="2">1.1.1.304</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_53638</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(S)-acetoin + NAD(+) = diacetyl + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27286"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15687"/>
            <dbReference type="ChEBI" id="CHEBI:16583"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.304"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.304" evidence="2"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019182717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3E3"/>
    <dbReference type="STRING" id="683960.A0A1E3P3E3"/>
    <dbReference type="GeneID" id="30199963"/>
    <dbReference type="OrthoDB" id="47007at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052588">
        <property type="term" value="F:diacetyl reductase ((S)-acetoin forming) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045150">
        <property type="term" value="P:acetoin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014007">
        <property type="entry name" value="23BDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02415">
        <property type="entry name" value="23BDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005559">
        <property type="entry name" value="PRK07231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF121">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="15"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="188"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR614007-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR614007-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="262" mass="27940" checksum="99CE710F72EA27FC" modified="2017-01-18" version="1">MTSRKVTALVTGAAQGIGKAIAIQLAKDGYQVAITDLPFQKAKALETVKEIETIGSKSLFIPCDSSKKNEVFDAVEETYQTFGSFNTMVNNAGIATIAPLLETTEEQINKILNVNINGVVYGIQAAAKKFEQLGNQPGKIINAASIASYEAGVMLGLYSATKFAVRGLTQSAAKELASKKITVNCYCPGIVMTPMWDEIDKKSCEITGAPRGSVTKRALDKIVLGRCSEPQDVANLVSFLASEKADYMTGQAIVVDGGSIYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3H9</accession>
    <name>A0A1E3P3H9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_92138</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039248.1">
        <property type="nucleotide sequence ID" value="XM_019186347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3H9"/>
    <dbReference type="STRING" id="683960.A0A1E3P3H9"/>
    <dbReference type="GeneID" id="30203593"/>
    <dbReference type="OrthoDB" id="2943660at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051430">
        <property type="entry name" value="Fungal_TF_Env_Response"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31944">
        <property type="entry name" value="HEME-RESPONSIVE ZINC FINGER TRANSCRIPTION FACTOR HAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31944:SF131">
        <property type="entry name" value="HEME-RESPONSIVE ZINC FINGER TRANSCRIPTION FACTOR HAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="19"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="179" mass="20272" checksum="6EF0867EBE68BC9C" modified="2017-01-18" version="1" fragment="single">MSSEDEATRPKKRQRMSVVCLNCKARKIKCDKKRPSCTNCVKCNVGHLCRYEAPHWVNRVINTEGQSKLDNGEKELLPQVLTGPNSGGNEAALQEEVKRLRNRLESIENSLVQSPDSSSQKSIKREYEDDIIDFYGAYNSLTIKRSGMLDHKPLSSSAITKKDHFLSLVSGYMFLCNFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3J5</accession>
    <name>A0A1E3P3J5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyridoxamine kinase/Phosphomethylpyrimidine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_94868</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038679.1">
        <property type="nucleotide sequence ID" value="XM_019186468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3J5"/>
    <dbReference type="STRING" id="683960.A0A1E3P3J5"/>
    <dbReference type="GeneID" id="30203714"/>
    <dbReference type="OrthoDB" id="10028886at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008902">
        <property type="term" value="F:hydroxymethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19367">
        <property type="entry name" value="TenA_C_ScTHI20-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004305">
        <property type="entry name" value="Thiaminase-2/PQQC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00097">
        <property type="entry name" value="HMP-P_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858:SF17">
        <property type="entry name" value="HYDROXYMETHYLPYRIMIDINE_PHOSPHOMETHYLPYRIMIDINE KINASE THI20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03070">
        <property type="entry name" value="TENA_THI-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="2">
        <location>
            <begin position="28"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Thiaminase-2/PQQC" evidence="1">
        <location>
            <begin position="323"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="531" mass="57683" checksum="386F13CF019B4966" modified="2017-01-18" version="1">MSTTVVPITWPKLEPSKLSTVLTVAGSDSSGGAGIEADIKAITANGAYALTAITALTAQNTKGVDSVVYTDKTQFEKILNANFSDIDIDVVKTGLLTVDAIDVLADFVSKYHVGKPFVIDPVLVATSGATLGKKEVLLRAIDKLIKKATLLTPNFIEAKEILKHLDLDFEVENLEDLKKAAKIIQSKTGVVSVLLKGGHIPWNSQNKVAKEGEEAIITDVLYHSVDDSFTVYESDFVITENTHGTGCTLASSISANLAKGYDVKHAIDSAIRYVHGGIVAASKIGHGNGPLNHVYHIVSTPALTHSLSTLFLEGHFVDFLKSHPTVTPIWDMYVNHPFVAQVANGSLPKHKFVHFLKQDYAYLINYARVHSLAASVAPDIGCIQREAGILENIAHEMKLHVGKLAKHGITDLTSLKLSRAGKEYADYLMAIAKGGDWFEINVAMAPCLFGYSEAAFNAEVTVGEGEYLEWINDYKSEGYSDAVELGRITLERHSIGISTDKALKLVKIFADVALLEVNFWTDALNFEEETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3Q7</accession>
    <name>A0A1E3P3Q7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL13m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_68661</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039333.1">
        <property type="nucleotide sequence ID" value="XM_019184130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3Q7"/>
    <dbReference type="STRING" id="683960.A0A1E3P3Q7"/>
    <dbReference type="GeneID" id="30201376"/>
    <dbReference type="OrthoDB" id="274622at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1180.10:FF:000007">
        <property type="entry name" value="50S ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_uL13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_uL13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_uL13_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01066">
        <property type="entry name" value="rplM_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="163" mass="18657" checksum="10D114C17ACAE025" modified="2017-01-18" version="1">MSQKIGRTSLAFARLWHHVDVAQETRSVGRLASSIAITLMGKHKPVYHNSNDMGDYVVVTNCKKLRVTGNKFEDKEYWKHSGKPGHLILTPMEKIVADKGFGEVLKRAVSGMLPKNKLRKHRLDRLKVFDGSDHPYKQNLYAVHDQQPLVREKLQQLESKNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3Y2</accession>
    <name>A0A1E3P3Y2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_92108</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="3"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039215.1">
        <property type="nucleotide sequence ID" value="XM_019186340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3Y2"/>
    <dbReference type="STRING" id="683960.A0A1E3P3Y2"/>
    <dbReference type="GeneID" id="30203586"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.300:FF:000006">
        <property type="entry name" value="Beta-glucosidase H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000495">
        <property type="entry name" value="Periplasmic beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050288">
        <property type="entry name" value="Cellulose_deg_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715:SF27">
        <property type="entry name" value="BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07691">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00758">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA14" evidence="6">
        <location>
            <begin position="398"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="837" mass="92793" checksum="53082BA28F2C6955" modified="2017-01-18" version="1">MSDFDVEKVLEQLTLTEKIGLTAGIDFWHTYPVERLNIPSLRFSDGPNGVRGTKFFNGVKGAVFPNGTGLAATFDKDLLREAGNMMALEGKHKGAHVILGPTTNMQRGPNGGRGFESFSEDPYLAGIASAAIVNGIQDKHIAATIKHYTGNDLEHERNSSDSIISPRALREIYLEPFRLAVKYSNPLCFMTAYNKVNGEHASQSKFLLQDVLRGEWKWDGAVMSDWYGTYSTSQALENGLDIEMPGPPRMRTELAISQQVSSKELHINHLNDRVRAVLNLVKYAKQGDIPENAPEDEKNNTSETAEFLRKLAAESIVLLKNDNNVLPLKKEDKIAVIGPNAKYGAFSGGGSASLAPYYVVTPYEGIANKIGSNPAYTVGAYAHDTLPGLSEQAINPKTGKPGIHMSFYHEPRGTPGRELFDELDLTTSYHIMFDYDNKAVRDYLFYADLECVFTPEETADYDFGVTVLGTAQLFVDDKLVVDNKENQKKGQAFFNTGTVEEVGSVRLEAGKKYNVRIEFGSGKTRTYTEQDGVDFVGNGAIAFGVAKAIDPEGEIKKAVELAKSVDKAIVVIGLNREWESEGYDRKDLTLPGYTDKLVEAILDANPNTVIVNQSGTPVEMPWIKKAKTLVHAWYGGNELGNAIADVLYGDVNPSAKLSLSFPIKIEDNPAFLNFHTERGRVLYGEDIFIGYRYYEKLQRRVAFPFGYGLSYTTFKFSKLSNSVDESKDELKVSVNVQNTGSVKGKEVVQVYIRKVDSDVIRPIKELKGFEKVELDAGSNKTVELKLSLKDSVSFFDEFQDKWSVQAGEYEIHVGASSDDIELIDKFNVKKSFFWSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3Z4</accession>
    <name>A0A1E3P3Z4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mannose-1-phosphate guanylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_31237</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in cell wall synthesis where it is required for glycosylation. Involved in cell cycle progression through cell-size checkpoint.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>alpha-D-mannose 1-phosphate + GTP + H(+) = GDP-alpha-D-mannose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="EC" id="2.7.7.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.13" evidence="3"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039280.1">
        <property type="nucleotide sequence ID" value="XM_019181812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3Z4"/>
    <dbReference type="STRING" id="683960.A0A1E3P3Z4"/>
    <dbReference type="GeneID" id="30199058"/>
    <dbReference type="OrthoDB" id="1733332at2759"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00930"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004475">
        <property type="term" value="F:mannose-1-phosphate guanylyltransferase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06425">
        <property type="entry name" value="M1P_guanylylT_B_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000013">
        <property type="entry name" value="mannose-1-phosphate guanyltransferase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045233">
        <property type="entry name" value="GMPPB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="10">
        <location>
            <begin position="2"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="11">
        <location>
            <begin position="252"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ60073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="362" mass="39710" checksum="2524559177E2FEB1" modified="2017-01-18" version="1">MKGLILVGGYGTRLRPLTLTVPKPLVEFANRPMILHQIEALANAGVTDIVLAVNYRPEVMVETLKKYEQEYGVSITFSVETEPLGTAGPLKLAEDVLKKDKSPFFVLNSDVICEYPFEELAKFHNAHGGQGTIVATKVDEPSKYGVIVHDISTPNLIDRFVEKPVEFVGNRINAGLYILNPEVIDLIELKPTSIEKETFPQLVEQKSLYSFDLEGFWMDVGQPKDFLAGTGLYLTSLARRSPEKLTTGKDYIDGNVLIDPSAKIGEGAKIGPNVVIGPNVTIGNGVRIKESVVLSNSTIKEHALVKNTLVGWNSEVGRWARLEGVTVLGDDVKVKDEIYVNGGKVLPHKTISANVEKESIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P402</accession>
    <name>A0A1E3P402_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_78837</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039433.1">
        <property type="nucleotide sequence ID" value="XM_019184793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P402"/>
    <dbReference type="STRING" id="683960.A0A1E3P402"/>
    <dbReference type="GeneID" id="30202039"/>
    <dbReference type="OrthoDB" id="1894652at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21203">
        <property type="entry name" value="ECM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133683" description="ER membrane protein complex subunit 10" evidence="1">
        <location>
            <begin position="21"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="20388" checksum="08A6A3B797013D15" modified="2017-01-18" version="1">MLNIIRISVLLASFVSLALATAPVQLYAHSLETHELSINLGVLTYNETSGSTQVIDAPELPEFEEGDYCIGAEINGNFQCHSYARLEFPLRYELIISTKPKTGELISMSFRRAENEGIKSIIKPAVPGAQPATKEAPKVVKNAEGKEVLEEEEPPKSFIQKYWMYIVPALIMFMISSGQEPQGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P467</accession>
    <name>A0A1E3P467_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15 16">tRNA ligase</fullName>
            <ecNumber evidence="1 16">6.5.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">WICANDRAFT_31162</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">One of the two proteins required for the splicing of precursor tRNA molecules containing introns. The ligation activity requires three enzymatic activities: phosphorylation of the 5' terminus of the 3' half-tRNA in the presence of ATP, opening of the 2'3'-cyclic phosphodiester bond of the 5' half-tRNA leaving a 2'-phosphomonoester and ligation of the two tRNA halves in an ATP-dependent reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 16">
            <text>ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m = (ribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="14 16">Belongs to the TRL1 family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.3" evidence="1 16"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039201.1">
        <property type="nucleotide sequence ID" value="XM_019181803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P467"/>
    <dbReference type="STRING" id="683960.A0A1E3P467"/>
    <dbReference type="GeneID" id="30199049"/>
    <dbReference type="OrthoDB" id="276239at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051730">
        <property type="term" value="F:GTP-dependent polyribonucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003972">
        <property type="term" value="F:RNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036498">
        <property type="term" value="P:IRE1-mediated unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032056">
        <property type="term" value="P:positive regulation of translation in response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000622">
        <property type="term" value="P:regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001934">
        <property type="entry name" value="tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019039">
        <property type="entry name" value="T4-Rnl1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012387">
        <property type="entry name" value="Trl1_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015966">
        <property type="entry name" value="tRNA_lig_kin_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015965">
        <property type="entry name" value="tRNA_lig_PDEase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32004">
        <property type="entry name" value="TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32004:SF1">
        <property type="entry name" value="TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09511">
        <property type="entry name" value="RNA_lig_T4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08302">
        <property type="entry name" value="tRNA_lig_CPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08303">
        <property type="entry name" value="tRNA_lig_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019634">
        <property type="entry name" value="tRNA_lig_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 16" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="T4 RNA ligase 1-like N-terminal" evidence="20">
        <location>
            <begin position="63"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA ligase kinase" evidence="19">
        <location>
            <begin position="391"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA ligase phosphodiesterase" evidence="18">
        <location>
            <begin position="563"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="17">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00055002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR019634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR019634-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF08302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF08303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF09511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ59994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="819" mass="94546" checksum="9FA62297D5C6ED28" modified="2017-01-18" version="1">MSLQNDPNCVQELIEKLEEATTFKKRGKATKRVLDILDSEQKLISWKFNEWDYAKDKIKLPSNARGLFTLLDEPKIAVRGYDKFFNINEVYSTRWEWLEQNTTGPYIVTAKENGCIILISGLEDGTIVVCSKHSTGNREDTTKNHAIAGQLFLEEQLKQLNLTTKDLALKLYELNLTAVAEFCDDGFEEHIIEYKGRDAGLYLHGLNLNKPIFETYSIEKVENFADSWGFKKIDYFIEKNIGDLKLKLDDYSKSGSYNGKEIEGFVIRCKKAEDSSDYFFKFKFEEPYLMYRQWREVTKEYITHKQRSSIKFTKHKFITNKYLDFVIPLLEEEPTVAKDYISGFGIISLRNRFLQHYGKSGPEILNQELLEELEVLNSLDALKIDEKTKFVIIPVATIGCGKTTTAQTLVNLFKNSWGIISNDDIPNGKSAKVVFVKKALEILSTKKVVVLDRNNHQYRERQQIFDDFAAFRDDYFPHDTNVQFICLNFIPEDSNDKELWDTTVQRVFERGDNHQSIKASSESAKVKGIMSGFIKRFQPVITEKEPDSKFHQVIDLNPSGQNSSLTNAEKVLSELNQKYPILVTKVPSVEEVQIAFTSALEYKPNYNKVMKKFGRQSNSQNHEKTKSKEKQRKPVFFSIDIPQRQRIISGIDELIQKKAPFLNIENSSWCYLKENNRIQGEFHITLVHVNQGKRGNEHDRNVWANYLTMYESLGSKFESEVFADISLKKLIWNKKIVTILVNVLQIYHKDKILSGLGVANKHPHITVGLVSNEVKPVYSNTLAEEASFETGEGLFDNNLNVLHWEDEFVLKKLPLKVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P482</accession>
    <name>A0A1E3P482_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">Pre-mRNA-splicing factor PRP46</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Pre-mRNA-processing protein 46</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_83775</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in pre-mRNA splicing and required for cell cycle progression at G2/M.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 8">Belongs to the WD repeat PRL1/PRL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038894.1">
        <property type="nucleotide sequence ID" value="XM_019185593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P482"/>
    <dbReference type="STRING" id="683960.A0A1E3P482"/>
    <dbReference type="GeneID" id="30202839"/>
    <dbReference type="OrthoDB" id="10256122at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000012">
        <property type="entry name" value="Putative pleiotropic regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045241">
        <property type="entry name" value="Prp46/PLRG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19923:SF0">
        <property type="entry name" value="PLEIOTROPIC REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19923">
        <property type="entry name" value="WD40 REPEAT PROTEINPRL1/PRL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="1 7 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="138"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="180"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="222"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="264"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="430"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00026147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU369036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="453" mass="51784" checksum="D128244FCE5C62E9" modified="2017-01-18" version="1">MFLGSLLTISHRKPLMCYEKELHNCSKIQNHHYLMIKNRKEYKHTYELPAYLQEKINSKTKKTAPKLETEEEAEDQLIIRTQQPSPADLDTNGNQLVLHDPKIEHNASRALVQRQQHQQFYIPEKPEWHAPWKLMRVIGGHKGWVRSLAVEPENKWFASGSNDNTIKVWDLASGQLKLTLTGHVMTVRDIAISDRHPYMFSCGEDKTVKCWDLERNRVIRDYYGHLSGVYSLDVHPTLDVVVTGSRDSTVRVWDMRTSTGIFTLSGHTQPLTKVKCQATDPQIISTSNDKTIRLWDLAAGKTQTVLTHHKKPIRGLALHPTQYAFATASPDNIKQWKFPEGSFMHNFMPRHEGIINTLSINQDNVMFSGGNNGSLAFFDWNSGHKFQELQTTTMPGSLESEAGIFSSTFDKTGMRLITGEADKSIKIWRENPDATPESDPGIPFDPEQFRDRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4V3</accession>
    <name>A0A1E3P4V3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_63001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039652.1">
        <property type="nucleotide sequence ID" value="XM_019183474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4V3"/>
    <dbReference type="GeneID" id="30200720"/>
    <dbReference type="OrthoDB" id="1687175at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="624" mass="71045" checksum="F44C3D9B68C0B6A9" modified="2017-01-18" version="1">MSLLALPDEILLRICNLLDVYDLSSLAKVPVIGPRIARFYMFYHDETTEARALARKLEDNSPVYVYSLEDSPEDYDPSEIWSTFIILGQPNDQIILDKIKDMPQKASIKIICTSKSLVNLIESRSIFESCRNTVSICFLNLWELGMVQITGLDPGSLDFPELHELTLVCCTATNDTSKFKLPKISSILFGCSTESLSILKNFDFEKYDLLQLIKLTFLDFQNDVNIGDITFGKAKEVHLALNHNGFPTNIRGHLKNVSMEHVSSLVLTDFTSLIDIDAPNLTEMTIDINTSSFTKVKFLNFNAPKLLRIGTTNPDFVLFDTFENFNAPLLKEIDINELSLRDNPDYVFENLRTISTAEYTLWHGKFNSLVLTTLEISLGPSMELYNDLKATDFPSLRNLQIHSHSPCDLSKIPFGKTYPSLLRLEFFGFYNVQSDTLNQLLSRCPNLKELEMEPQDAAITIDGVSSDTLEDIRIFPNEDTFQKFEMTNCHFKKLKKFFIAPDTDTSMPDQFDRFSGRIEMIAPNLESILVGLEMESIDISKFKTTKLKRVLLRGPVSEIELGDIQDLKDLNLFYPFTKLGFGQEPAALHNFTLPDRLNFPAVSEYAVMRLMSIYRNTALKRNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P581</accession>
    <name>A0A1E3P581_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Nuclear transport factor 2</fullName>
            <shortName evidence="3">NTF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_77265</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Has a role in nuclear-cytoplasmic transport of proteins and mRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039792.1">
        <property type="nucleotide sequence ID" value="XM_019184527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P581"/>
    <dbReference type="STRING" id="683960.A0A1E3P581"/>
    <dbReference type="GeneID" id="30201773"/>
    <dbReference type="OrthoDB" id="6507044at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00780">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000005">
        <property type="entry name" value="Nuclear transport factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045875">
        <property type="entry name" value="NTF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12612">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NTF2" evidence="4">
        <location>
            <begin position="8"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00026247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU369002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="125" mass="14222" checksum="75479D88792B3AF8" modified="2017-01-18" version="1">MSVDFNTLAQQFTEFYYNQFDSDRSQLGNLYRDQSMLTFETSQVQGAKAIVEKLTALPFQKVGHRITTLDAQPASPNGDVLVLVTGDLLIDEEQNPQRYSQVFHLIPEAGSYFVYNDIFRLNYGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5Y1</accession>
    <name>A0A1E3P5Y1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Thiamine transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_61221</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039861.1">
        <property type="nucleotide sequence ID" value="XM_019183131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5Y1"/>
    <dbReference type="GeneID" id="30200377"/>
    <dbReference type="OrthoDB" id="2018619at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11482">
        <property type="entry name" value="SLC-NCS1sbd_NRT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012681">
        <property type="entry name" value="NCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00800">
        <property type="entry name" value="ncs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF15">
        <property type="entry name" value="NICOTINAMIDE RIBOSIDE TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="391"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="476"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="555" mass="62610" checksum="1A095A1053C8ABF0" modified="2017-01-18" version="1">MSYMRKFLKAIEVPVGERETLSVLRNPDLQPIKPEFRVWGFWSNFAYWGIISFSMGTWLSASASLSLGLSVREIIGGYILGDILTLCFTLSNSFPGTDWHIGYTVCQRIAFGIYGSGLGILIRVLLSIVGAGSNAWLGGLACSLLLSAWSKDYLHMKNTFSEGIAMTRRDFVGFIIFQVITCICYWVKPHKMNVPLITSCFACFFAMLGMVIFLVNKADGYGELFQLETELSGSKRAWTWVYIITIWFSSVSPGSTNQSDYSRFASSKTKLWIGTILALLLPTTIIPLFGIIGASCTKAAYGEQMWVPTDIVLYWLNEHYDAKTRCAATFIAFAFIFSQLCLNVVTNGFAGGMDLAGILPKYINIKRGALLTTALSFAVQPWNFYNTSSSFLTVMSSFGVITTPIISIIVCDYFIIRKMRYRISHAYELKGDYYYTYGVNWRAYIAFVCGVGPGLPGMAWQINNHYFTNAQGIINFYYGDSFFAFLISFTLYWFLCWIKPVQISQAVDDKDYYNALTEKEQIKYGIEPNEDVVSEHSSEEFPETKLEKVVQKLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P676</accession>
    <name>A0A1E3P676_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_83126</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Endoplasmic reticulum membrane</location>
            <topology evidence="2 10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the OST1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040007.1">
        <property type="nucleotide sequence ID" value="XM_019185494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P676"/>
    <dbReference type="STRING" id="683960.A0A1E3P676"/>
    <dbReference type="GeneID" id="30202740"/>
    <dbReference type="OrthoDB" id="310030at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007676">
        <property type="entry name" value="Ribophorin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049">
        <property type="entry name" value="RIBOPHORIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04597">
        <property type="entry name" value="Ribophorin_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009027340" description="Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1" evidence="10">
        <location>
            <begin position="18"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="465" mass="53136" checksum="E594AE3E63660B17" modified="2017-01-18" version="1">MKLTLLALSFLLTIVSCSFIPDQSWENVEFKRTIDIAKSYSKEKIDLVIKNVDSKPNAEYYYAVPSYAFDKLSVYAFFYANGLKTLSSTLINDTTTVNNEPLVNYIKVQLPQPIAPKQTFKLSANFAVTDCLEPYPHKIELTDEQFLLLKTNKLPISAYKTLDSSLAIGGISDGKELELEGKENPYKGVVKNQQLTYEFNEEISPFKFTVLNLLYRHNLPLTKVTSLQRGVWVSHWANSLQFEEFYKLTNKAAQLKNGFSRSDYMMNRQMLKQSSSLAAIELKLPEESKDVYYTDLVGNVSTSRVFSDRLYIKPRYPIFGGWNYNFTIGWTNSLTEHLRQSGSDEYILAVPFINGPVDVSYDEIEISFYLPEGAKLLDIASVIPYEKKTEGHELSYFDLSNGHTKITLTFNNIIDDLSHLELVIKYEYGFWSFIKKPLDIAKVVFIALISYLALTKVDISLKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6G5</accession>
    <name>A0A1E3P6G5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein mL59 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_61590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040232.1">
        <property type="nucleotide sequence ID" value="XM_019183205.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3P6G5"/>
    <dbReference type="GeneID" id="30200451"/>
    <dbReference type="OrthoDB" id="18529at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037507">
        <property type="entry name" value="Ribosomal_mL59"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040922">
        <property type="entry name" value="Ribosomal_mL59_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28041">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28041:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18126">
        <property type="entry name" value="Mitoc_mL59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Large ribosomal subunit protein mL59" evidence="1">
        <location>
            <begin position="25"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="170" mass="19928" checksum="A0289B756EB54BD6" modified="2017-01-18" version="1">MRVSLGLFNTAVKTEISYFNTLPEKLQRFFTRYPPRPFKEYASESTLTTAENANPFIANKHPITKRYHTAKYSMRRQSDLWKIAYRHGIQDSLPPLANGKKFYQEKYDTKPLMKGVLRPKGAKYERERPLKEAKMKEALLHADEKIAELRGKKKIENRLAKKAKKAKHFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6J6</accession>
    <name>A0A1E3P6J6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Gfo/Idh/MocA-like oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_90300</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040263.1">
        <property type="nucleotide sequence ID" value="XM_019186227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6J6"/>
    <dbReference type="STRING" id="683960.A0A1E3P6J6"/>
    <dbReference type="GeneID" id="30203473"/>
    <dbReference type="OrthoDB" id="3978523at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051450">
        <property type="entry name" value="Gfo/Idh/MocA_Oxidoreductases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377:SF1">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase C-terminal" evidence="2">
        <location>
            <begin position="139"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="42345" checksum="834370EDFFA41565" modified="2017-01-18" version="1">MSQLKLIVCGLGLIGPRHAEQIAQNKETELIAFIDPSPKAQLIADQFNVPLYSSLTELLNAGLRPDAAYVCTPNALHVSVALELANESIHILVEKPISTKVEDAIALKDCVESKGVKLLVGHHRRFNPYILATKKHLSKLGAIIAVDGVWTLKKNDTYFKQVYWRNVKSLGGGPILINLVHDLDLLQYLMGPIKRVYAEKLSQHRDIVESDDPVDEGAVLTLSFKSGAKGTFIVSDNVISPFNFEMGTGENPLIPKVEDPTDGVFYRIFGTKGTLTVPDFHYYHQNDLKPGLEKGWWEPLHKTVLEKDISGIPFALQLDHFVKYVRGEEDAKCTITDGISALLVIEAIMESLESGAPRNVKSVSSFTSFLKNKDEDPIKSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6Q9</accession>
    <name>A0A1E3P6Q9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator Rua1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_61209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039849.1">
        <property type="nucleotide sequence ID" value="XM_019183128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6Q9"/>
    <dbReference type="GeneID" id="30200374"/>
    <dbReference type="OrthoDB" id="3982958at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028012">
        <property type="entry name" value="Rua1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14616">
        <property type="entry name" value="Rua1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator Rua1 C-terminal" evidence="1">
        <location>
            <begin position="91"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="429" mass="49497" checksum="B5A8AA469A0D8668" modified="2017-01-18" version="1">MFKVLDQLKTRDGKIPYEFLDVSYQNSTFHDYTLESFKEKYVSIYGEDGIVKNLQRYETMPNSRIIHENQICYKVVESFSSPKTKLGKPKVNTYILCPLCDPFKNSHLKPSFTSKIRDGQRVAVDQNQPQFYDTLHSQYEHHLSQVHGVMRFGAAANPFVGLSLTQGKVNAETPVYELNCICPYSLPSAYPCLAEHRLKVLDRNDNPFKAYFRHVHLQHYNLSSKNPDFVYKPEISDVNGNVYLNIFIPLSVQKFQSSLRHLKDCCAFTDRAPILCKDQDLLSLVASYVSTTPKKESTLNDPYSQTPSLKRKRSLSPTITIEDLRNFDSMKRIKPSKNPCVNRNDGKIFTDTKVEETGSTHSVQLLGDKYLAKELMKGHRQRFFGYKHPSAEQNQKEVQRAMESLREYAGIDFFPFSSFRVKRPSKCFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6Y1</accession>
    <name>A0A1E3P6Y1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">UDP-N-acetylglucosamine transferase subunit ALG14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Asparagine-linked glycosylation protein 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ALG14</name>
        <name evidence="12" type="ORF">WICANDRAFT_28221</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway. Anchors the catalytic subunit ALG13 to the ER.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 11">Heterodimer with ALG13 to form a functional enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ALG14 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040398.1">
        <property type="nucleotide sequence ID" value="XM_019181461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6Y1"/>
    <dbReference type="STRING" id="683960.A0A1E3P6Y1"/>
    <dbReference type="GeneID" id="30198707"/>
    <dbReference type="OrthoDB" id="17098at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098548">
        <property type="term" value="C:cytoplasmic side of Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043541">
        <property type="term" value="C:UDP-N-acetylglucosamine transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004577">
        <property type="term" value="F:N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013969">
        <property type="entry name" value="Oligosacch_biosynth_Alg14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12154">
        <property type="entry name" value="GLYCOSYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12154:SF4">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE TRANSFERASE SUBUNIT ALG14 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08660">
        <property type="entry name" value="Alg14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ61191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="221" mass="24711" checksum="0B2F3621A376EC6B" modified="2017-01-18" version="1">MQVTFVVVGVLAVAALLVIRLAFVLPALEHHQLRKKPLKIIEEKSIMVLLGSGGHTGEMIRILSSTESVTKAKKLYWVISSGDSSSSIHLQKLQNELNISERSKTIELPRARKVGEGLISSIGSTIKTIISTFKQFIQMKAFPDVLMVNGPGTSVPICYFFFILKLFGIHSTKILYIESLARVSDLSLSGKLCYLISDRFIVQWEKLQTRYPNAEWYGILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6Z0</accession>
    <name>A0A1E3P6Z0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ferrochelatase</fullName>
            <ecNumber evidence="7">4.98.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_83392</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the ferrous insertion into protoporphyrin IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>heme b + 2 H(+) = protoporphyrin IX + Fe(2+)</text>
            <dbReference type="Rhea" id="RHEA:22584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="4.98.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Porphyrin-containing compound metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ferrochelatase family.</text>
    </comment>
    <dbReference type="EC" id="4.98.1.1" evidence="7"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040392.1">
        <property type="nucleotide sequence ID" value="XM_019185534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6Z0"/>
    <dbReference type="STRING" id="683960.A0A1E3P6Z0"/>
    <dbReference type="GeneID" id="30202780"/>
    <dbReference type="OrthoDB" id="1323at2759"/>
    <dbReference type="UniPathway" id="UPA00252">
        <property type="reaction ID" value="UER00325"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00419">
        <property type="entry name" value="Ferrochelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03411">
        <property type="entry name" value="Ferrochelatase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1400:FF:000001">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1400">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00323">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001015">
        <property type="entry name" value="Ferrochelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019772">
        <property type="entry name" value="Ferrochelatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033644">
        <property type="entry name" value="Ferrochelatase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033659">
        <property type="entry name" value="Ferrochelatase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00109">
        <property type="entry name" value="hemH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108:SF1">
        <property type="entry name" value="FERROCHELATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00762">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53800">
        <property type="entry name" value="Chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00534">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0408">Iron</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 7" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="349" mass="39426" checksum="0B658EAD9B916D33" modified="2017-01-18" version="1">MGGPSTIPEVHDFLYKLFADGDLIDLGVGRFQRLLAKFISARRTPKIEKYYQEIGGGSPILKWSQYQAEEACKILDKSNPETAPHKPYVAFRYARPYTEDTLQKLLDDGITRAVAFSQYPQFSYSTSGSSINELWRQTQKLDPQRKIEWSVIDRWPDHPGLVKAFAGNIKEKLAEFPESIRDKVKILFSAHSLPMDVVNSGDAYPAEVAATSYAVMKELNFSNPYRVVWQSQVGPKPWLGAQTAKLVERIEADSEGIVLVPIAFTSDHIETLHEIDLELIGESKYKDRIKRAESLNGNQTFIEGLADIVGSHLKSDELYSKQLPFDCQLGVGRDVFKHPSELFGDHYKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P730</accession>
    <name>A0A1E3P730_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Coupling of ubiquitin conjugation to ER degradation protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_29271</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the CUE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040365.1">
        <property type="nucleotide sequence ID" value="XM_019181591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P730"/>
    <dbReference type="STRING" id="683960.A0A1E3P730"/>
    <dbReference type="GeneID" id="30198837"/>
    <dbReference type="OrthoDB" id="3824970at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14424">
        <property type="entry name" value="CUE_Cue1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000050">
        <property type="entry name" value="Related to AMFR protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="CUE" evidence="11">
        <location>
            <begin position="68"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="33"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="108"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="33"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="50"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="166"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ61158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="212" mass="23531" checksum="AD715F6858EB3D29" modified="2017-01-18" version="1">MDSSTITFIATLVIAFIFLRWFISSDEEDEATIQSTATSTSPSQSQAQDSQHDAEEQSTRQERLRRPVTNSMIEVVQQLAPHLTVGQIKLDLQRTGSVQETVDRFLSEGTLPYPEGEGPNASRTANPAQDSRNQVDNIKPENLLKKYGINESKSEESTAAASGSSTKEKGWSESAEDRASTLQQKKVDMIVKARKRLESQLKNEQDLSKLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7H2</accession>
    <name>A0A1E3P7H2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein AHC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_77943</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040515.1">
        <property type="nucleotide sequence ID" value="XM_019184638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7H2"/>
    <dbReference type="STRING" id="683960.A0A1E3P7H2"/>
    <dbReference type="GeneID" id="30201884"/>
    <dbReference type="OrthoDB" id="5355528at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058707">
        <property type="entry name" value="AHC1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058706">
        <property type="entry name" value="zf-C2H2_AHC1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25910">
        <property type="entry name" value="AHC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25909">
        <property type="entry name" value="zf-C2H2_AHC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AHC1 N-terminal" evidence="3">
        <location>
            <begin position="54"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="AHC1-like C2H2 zinc-finger" evidence="2">
        <location>
            <begin position="161"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="445" mass="49565" checksum="BA95236911606BC7" modified="2017-01-18" version="1">MSQESGYNFNSSGSDHDQNKSNIYLQIATPKSPNSDGFSPTEIHDEEHSLEDEIKQNVINQIDVEILLKHREYNLVQNEISKVQTQLDTLQKLHDDPAYSKYVQNVIDMRDGVKNELNNYNTSTNDSEIITRRHSFYNIRKSGSEEPPKPHRTSYGGLRPVVDSNGNKICVHKRSDGVIVKIECPTCARSDFGSAQGFLNHARLAHQVEYKSQDHAALVCGAILPDIEQDDIGIASIQKLKDMGIDADKNLAPGVFTNGSIGSSDEMSGPQKKKARRSISKTGVPSGYLEKLYNKEVSDEGTFKEIYQDATTKTEVDFGDDDPEATTPPISQDSSTLENQQQSKLFKKGHNRRKSRGGLSAVTFDDQIPSTSSEHEVEKLSPRDITAQELIVEEHLNSSASTVRFSENSITFGEVDGLTPAQRRRVPTIPNPLRTRSRSSRENSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7P3</accession>
    <name>A0A1E3P7P3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">NEDD8-activating enzyme E1 catalytic subunit</fullName>
            <ecNumber evidence="8 11">6.2.1.64</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_61876</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalytic subunit of the dimeric E1 enzyme, which activates NEDD8.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.64"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.64" evidence="8 11"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040527.1">
        <property type="nucleotide sequence ID" value="XM_019183263.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7P3"/>
    <dbReference type="STRING" id="683960.A0A1E3P7P3"/>
    <dbReference type="GeneID" id="30200509"/>
    <dbReference type="OrthoDB" id="10255449at2759"/>
    <dbReference type="UniPathway" id="UPA00885"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019781">
        <property type="term" value="F:NEDD8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01488">
        <property type="entry name" value="Uba3_RUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.520:FF:000001">
        <property type="entry name" value="NEDD8-activating enzyme E1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.520">
        <property type="entry name" value="Ubiquitin activating enzymes (Uba3). Chain: B, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023318">
        <property type="entry name" value="Ub_act_enz_dom_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030468">
        <property type="entry name" value="Uba3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF6">
        <property type="entry name" value="NEDD8-ACTIVATING ENZYME E1 CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="12">
        <location>
            <begin position="29"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ61320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="329" mass="36623" checksum="207D5D089D079ADE" modified="2017-01-18" version="1">MSRSDLSIKPFLLQKGPFADDEFEPGQQVIDTIYNAKILVLGAGGLGCEILKNLACSGFRDIHVIDMDTIDLSNLNRQFLFRHSDIGRPKAVVAAEFVMKRVKGVKITPYYGKLQDKDEAYYKQFTLIISGLDSIEARRWINAKLVHMVDPDDFETVKPLIDGGTEGFRGQAKVIFPTFTACYECSLDTLSGQTTFPMCTIANNPRIPEHCIEYVSQIEWQQSHPGEKIDTDNPDHVLWLYEASLKRAKEFSISGITRSLVLGVVKNIIPAIASTNAIIAAACCNEAFKIVSSSNPILDNFMMYSGTDSVFTYSFAYERKDDCPICGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7X2</accession>
    <name>A0A1E3P7X2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Mannan endo-1,6-alpha-mannosidase</fullName>
            <ecNumber evidence="4 12">3.2.1.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_78127</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for normal synthesis of the cell wall.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>Random hydrolysis of (1-&gt;6)-alpha-D-mannosidic linkages in unbranched (1-&gt;6)-mannans.</text>
            <dbReference type="EC" id="3.2.1.101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyl hydrolase 76 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.101" evidence="4 12"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040710.1">
        <property type="nucleotide sequence ID" value="XM_019184668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7X2"/>
    <dbReference type="STRING" id="683960.A0A1E3P7X2"/>
    <dbReference type="GeneID" id="30201914"/>
    <dbReference type="OrthoDB" id="4187847at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008496">
        <property type="term" value="F:mannan endo-1,6-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007117">
        <property type="term" value="P:budding cell bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000006">
        <property type="entry name" value="Mannan endo-1,6-alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005198">
        <property type="entry name" value="Glyco_hydro_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014480">
        <property type="entry name" value="Mannan-1_6-alpha_mannosidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145:SF21">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DFG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03663">
        <property type="entry name" value="Glyco_hydro_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016302">
        <property type="entry name" value="Man_a_manosd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 12 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133722" description="Mannan endo-1,6-alpha-mannosidase" evidence="14">
        <location>
            <begin position="22"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="433"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR016302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ61503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="455" mass="50374" checksum="4325247630525B55" modified="2017-01-18" version="1">MILSSKLTFIALSTIIQAVYAVDLQISDKDSICSAASLVADGMMNYYSGSQYGGTVGMFQPPYYWWQAGHAFGGLLDHWYYCQNTTYENVLYSSLIAQTGTNYDYIPKNQSTTEGNDDQGFWGLFVMAAAERNFTEPHGDDKKVPSWLTMSQAVYNTMWKRWDRENCGGGLRWQIFTWNSGYNHKNTVSTACLFHMAGRLARFTGNDSYAEVADEVYQWLIDAHFLEEADAARVYDGADIADNCSNVVKLEWTYNYGLLLGGAAYLYNATQEDIWMHRVQNLVEGSKVFFNNSIMYERACQGGGTCNTDQRSFRAVFSRALGLTSLLVPSLYDDIRPLLEKSASAAAKSCSGGTDGITCGQDWTVNGWDNKYGLGEQMAALEVMLSLLPGTMPAPLDHNDSGVNTTGDYNAGLDSNTNQLDHEPLNIQTKDKAGAAIITAVVLFIIILGAIWMII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P820</accession>
    <name>A0A1E3P820_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Copper-fist domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_104158</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040224.1">
        <property type="nucleotide sequence ID" value="XM_019180400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P820"/>
    <dbReference type="GeneID" id="30197646"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.430.10">
        <property type="entry name" value="Copper fist DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001083">
        <property type="entry name" value="Cu_fist_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036395">
        <property type="entry name" value="Cu_fist_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00649">
        <property type="entry name" value="Copper-fist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01090">
        <property type="entry name" value="Copper-fist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00412">
        <property type="entry name" value="Cu_FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57879">
        <property type="entry name" value="Zinc domain conserved in yeast copper-regulated transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50073">
        <property type="entry name" value="COPPER_FIST_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Copper-fist" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="173"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="284" mass="31720" checksum="8FAA0A29615C16B4" modified="2017-01-18" version="1">MLYLGVLRFCCWKCRSGHRQLQCTHYDRAKNVFNVGQQGRPALDEERRAFKLVAADFDAEANPKCSESKLYKFQCRKNPRFRCCNGEIDIYLDPKKSKVVEIHPPMEIHSGSNGISVDAFQLPRRNGKAKFINPDSSKIKDNKSTQSVETGTVAEKTTHLLNVKLLPPPVSTNPPTTSITSPVSATRGRLTPKTQSATKRTIEREDTPIPSNSLSLSPSPSSQKRPCTSSKQTQADPVKELSSPNLPSQNTRSHAFENISNHVPSAPMEPLRTRITRRRSKSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8N2</accession>
    <name>A0A1E3P8N2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">3-hydroxyanthranilate 3,4-dioxygenase</fullName>
            <ecNumber evidence="9">1.13.11.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">3-hydroxyanthranilate oxygenase</fullName>
            <shortName evidence="9">3-HAO</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">3-hydroxyanthranilic acid dioxygenase</fullName>
            <shortName evidence="9">HAD</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Biosynthesis of nicotinic acid protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">BNA1</name>
        <name evidence="10" type="ORF">WICANDRAFT_26687</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>3-hydroxyanthranilate + O2 = (2Z,4Z)-2-amino-3-carboxymuconate 6-semialdehyde</text>
            <dbReference type="Rhea" id="RHEA:17953"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:36559"/>
            <dbReference type="ChEBI" id="CHEBI:77612"/>
            <dbReference type="EC" id="1.13.11.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the 3-HAO family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.6" evidence="9"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040980.1">
        <property type="nucleotide sequence ID" value="XM_019181290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8N2"/>
    <dbReference type="STRING" id="683960.A0A1E3P8N2"/>
    <dbReference type="GeneID" id="30198536"/>
    <dbReference type="OrthoDB" id="204928at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00330"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000334">
        <property type="term" value="F:3-hydroxyanthranilate 3,4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006569">
        <property type="term" value="P:L-tryptophan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06123">
        <property type="entry name" value="cupin_HAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000093">
        <property type="entry name" value="3-hydroxyanthranilate 3,4-dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00825">
        <property type="entry name" value="3_HAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010329">
        <property type="entry name" value="3hydroanth_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03037">
        <property type="entry name" value="anthran_nbaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15497">
        <property type="entry name" value="3-HYDROXYANTHRANILATE 3,4-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15497:SF1">
        <property type="entry name" value="3-HYDROXYANTHRANILATE 3,4-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06052">
        <property type="entry name" value="3-HAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 9" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>O2</name>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ61773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="174" mass="20044" checksum="4D1D823C64B105FC" modified="2017-01-18" version="1">MLPEPINIKKWIEENEHLLQPPVNNFCLHRGGFTVMIVGGPNERTDYHVNQTPEWFYMYKGDMTLRVVDEGKFRDITIREGDSFLLPGNTPHNPVRYADTIGLVVEQDRPEGVNDQIRWYCSNCSEIVHNITFYCSDLGTQVKEAILTFDNNIDLRTCKTCGTLNYSKPQVSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8U8</accession>
    <name>A0A1E3P8U8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_26046</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for vesicle fusion with the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041042.1">
        <property type="nucleotide sequence ID" value="XM_019181220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8U8"/>
    <dbReference type="STRING" id="683960.A0A1E3P8U8"/>
    <dbReference type="GeneID" id="30198466"/>
    <dbReference type="OrthoDB" id="10255013at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070300">
        <property type="term" value="F:phosphatidic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031321">
        <property type="term" value="P:ascospore-type prospore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15849">
        <property type="entry name" value="SNARE_Sso1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.70:FF:000008">
        <property type="entry name" value="Syntaxin family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF307">
        <property type="entry name" value="PROTEIN SSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00503">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="10">
        <location>
            <begin position="196"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="199"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ61835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="297" mass="33346" checksum="1744CF3E965351E4" modified="2017-01-18" version="1" fragment="single">YNNYGYDNSNPYQENYELNNYGAQQGAGGVATGASADDFVQYMNQISIINNDLDNYNQLIELIEFNQKKILNEINEEQELATRKQLDGLIAQASSLQQTLKVKIKDAQSKAGKDSAKKAQAENSRQRFLKLIQDYRVVEANYRDQNKQQAARQYKIVQPDATEAEVENAINDVGGQQIFSQAILNATRQGEAKTALAEVQSRHRELQRLEKTMAELTQLFHDMEELVAEQNVQVENVNQEVEGAQKDIELGVGHTTKAVDSARKARRKKCWCIIILIIVILIIIAVIVGAVAGKYAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8Z8</accession>
    <name>A0A1E3P8Z8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4112 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_61980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040629.1">
        <property type="nucleotide sequence ID" value="XM_019183290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8Z8"/>
    <dbReference type="STRING" id="683960.A0A1E3P8Z8"/>
    <dbReference type="GeneID" id="30200536"/>
    <dbReference type="OrthoDB" id="2103474at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025187">
        <property type="entry name" value="DUF4112"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35519">
        <property type="entry name" value="MEMBRANE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35519:SF2">
        <property type="entry name" value="PH DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13430">
        <property type="entry name" value="DUF4112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="252" mass="28344" checksum="4918826BCAEEFD27" modified="2017-01-18" version="1">MGIAGWAIKYISQRIFRENQLNKFGTEDPYYDVFEVEQQSYFGGAPRVKKIKKPKAIPVGISGNDERVLRKVLRRAYHLDMQFNVFGYRVGWTGIVGVVPVVGDIAGILFSLLLLKTARDVDGGLPLDVQAQFLFNIIVDFLIGLIPIVGDVVEIMYKANSRNALILEKHLNAKGEKNLGLTRDHGKLIDDTRSISSAAVSEDSEFDTTSPLKSPARYRSSPEENIKLRNQLKVGQDPNRVETVFKERTYSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9E8</accession>
    <name>A0A1E3P9E8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_59667</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040789.1">
        <property type="nucleotide sequence ID" value="XM_019182853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9E8"/>
    <dbReference type="STRING" id="683960.A0A1E3P9E8"/>
    <dbReference type="GeneID" id="30200099"/>
    <dbReference type="OrthoDB" id="3980623at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
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            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="410" mass="46587" checksum="FE4485AEA8D9F4A1" modified="2017-01-18" version="1">MGSCLSKEDAPASAQVSNKVQRTSKLDNPSSSSNEKDANDIAQNNKPLNGNNQQIIQQQQQQLSDTQQARKQSIVPSESSNGTSTAVNSVLLLGSGESGKSTILQQLKILHSNGFSDQELFDFKPFIFGNIILSAKDLVEAYKKFDIKLETDDQVDETDFEFISSATAPVDPHKSFDKRLALKIIALWKNQSTAELIKNHRQDFYLMDSAKYFFENLDRISQEDYLPTVTDVLRTRKKTSGIFDLKFELNGLKIHIYDVGGQRSERKKWIYCFDNVTSIIFCVALSEYDQTLLEEKTQNRLEESLTLFDSVVNSRWFSRCSIVLFLNKIDVFVEKLPYSPLENYFPDYSGGNDANKAAKYILWRFTRVNRSGLPIYPYITQATDTNNIKLVFVVVRESILSNILHDTGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9U0</accession>
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        <recommendedName>
            <fullName evidence="4">37S ribosomal protein MRP2, mitochondrial</fullName>
        </recommendedName>
    </protein>
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        <name evidence="5" type="ORF">WICANDRAFT_60227</name>
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    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS14 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041368.1">
        <property type="nucleotide sequence ID" value="XM_019182956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9U0"/>
    <dbReference type="STRING" id="683960.A0A1E3P9U0"/>
    <dbReference type="GeneID" id="30200202"/>
    <dbReference type="OrthoDB" id="413436at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1480:FF:000001">
        <property type="entry name" value="30S ribosomal protein S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_uS14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018271">
        <property type="entry name" value="Ribosomal_uS14_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19836">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19836:SF19">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US14M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00527">
        <property type="entry name" value="RIBOSOMAL_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00076896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="115" mass="13404" checksum="6E1D60A03B43440A" modified="2017-01-18" version="1">MGNFRFPVKFKLPTSYINHMVLRDHFKRLQAAEHEVTIQALKYIARNTQLPPRARLEAQLQLTTMPNYTRMTQIKDRCVASGSAKSVIKDFRLCRTQFREKARNGDLPGVKRGVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9V2</accession>
    <name>A0A1E3P9V2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_49532</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041402.1">
        <property type="nucleotide sequence ID" value="XM_019182631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9V2"/>
    <dbReference type="STRING" id="683960.A0A1E3P9V2"/>
    <dbReference type="GeneID" id="30199877"/>
    <dbReference type="OrthoDB" id="427368at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034085">
        <property type="term" value="P:establishment of sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057646">
        <property type="entry name" value="WD40_WDHD1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022100">
        <property type="entry name" value="WDHD1/CFT4_beta-prop_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048591">
        <property type="entry name" value="WDHD1/CFT4_hel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19932">
        <property type="entry name" value="WD REPEAT AND HMG-BOX DNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19932:SF10">
        <property type="entry name" value="WD REPEAT AND HMG-BOX DNA-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20946">
        <property type="entry name" value="Ctf4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12341">
        <property type="entry name" value="Mcl1_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24817">
        <property type="entry name" value="WD40_WDHD1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDHD1 first WD40" evidence="9">
        <location>
            <begin position="13"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="235"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="WDHD1/CFT4 second beta-propeller" evidence="7">
        <location>
            <begin position="402"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="WDHD1/CFT4 helical bundle" evidence="8">
        <location>
            <begin position="695"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="340"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="340"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ62195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="799" mass="88730" checksum="63F360B85D1D48B0" modified="2017-01-18" version="1">MVEPEVRNVYSSGGSTYVAYTKNGDHLLVAGSSGLIRNYKVGAEDSEPNNIDILDEISDLAVFGDDKVAVSSKIGEVELYSLKDNKAVGKVIRSSLPLRSTVFTHIGTMVAAAGDDDEVQIADLSDNTRVIKLKVNDQVHDISYNSTIDLISISLSNGEIKMYSLTSEEPQLLATIKDEIPKLIYQDDDEEAEDNVITTRVEWNPNGDHFAIPSESKDIKVFSRSENYKQVYSFPRLHQKNLADLKWSPNGDYLASADIGNTLIIWDSESRDTVLEKKLDSRVNNLAWAKNNADGYDLAVGSENGDAYIFSNVVKAKKAKGARLNGISALVDDEADISVDEGSDQEEANADDINSENELTNDEGDDGEKRADFFDDDDGFPERSIKRSRPSTFIGAKKYKLKPYSPGCTPYISDRRYLTINSIGYVSTVKQDSHSSITVSFFDTSVHREYHFEDLYGYDLASLNTEGILLGVSGNDSNITKILFRPHENVNEAWEKNIPTSSGEIITSISLSDSVILVCTSFGYIRSFTLFGLPIGLQKSSPVLASATNTNYMFTVSLSNNNQLIYNLQDLEGRFLQRDLTLPLELQNDADLLKGIFFSTYGDPVIVGTDGVILVLARWRTPLQATWVPILDSEEKVKEIGGKGDLSVWPLGLHANNLNCIIVRGSQFPTYPLPLPSELEIKLPTTKDSNQDDIAEEQFLRSRTMGELLGETLANDGEIFEEDNERLNEYAIAHDRAVLQLFANACQEGKNAKAWRLAREVKQDHGLVACNKIAERIGLVSLSNRINKLREQRMEEEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9Z1</accession>
    <name>A0A1E3P9Z1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 14">Sulfiredoxin</fullName>
            <ecNumber evidence="4 14">1.8.98.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_76297</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>S-hydroxy-S-oxy-L-cysteinyl-[peroxiredoxin] + [protein]-dithiol + ATP = S-hydroxy-L-cysteinyl-[peroxiredoxin] + [protein]-disulfide + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17545"/>
            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="Rhea" id="RHEA-COMP:13681"/>
            <dbReference type="Rhea" id="RHEA-COMP:17976"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:61973"/>
            <dbReference type="ChEBI" id="CHEBI:61974"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="1.8.98.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the sulfiredoxin family.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.2" evidence="4 14"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041327.1">
        <property type="nucleotide sequence ID" value="XM_019184360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9Z1"/>
    <dbReference type="STRING" id="683960.A0A1E3P9Z1"/>
    <dbReference type="GeneID" id="30201606"/>
    <dbReference type="OrthoDB" id="10023328at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032542">
        <property type="term" value="F:sulfiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032272">
        <property type="term" value="P:negative regulation of protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16395">
        <property type="entry name" value="Srx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1530.10:FF:000005">
        <property type="entry name" value="Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1530.10">
        <property type="entry name" value="Conserved hypothetical protein from pyrococcus furiosus pfu- 392566-001, ParB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016692">
        <property type="entry name" value="Sulfiredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21348">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21348:SF2">
        <property type="entry name" value="SULFIREDOXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017267">
        <property type="entry name" value="Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="ParB/Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="7 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB-like N-terminal" evidence="17">
        <location>
            <begin position="11"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="82"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Interchain" evidence="16">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR017267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR017267-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR017267-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ62120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="125" mass="13973" checksum="33175C28B0E0A6CE" modified="2017-01-18" version="1">MSIQSGSIGKLSYLPLNQIKRPIPPVLDYDKIDAMSSQLDGIPMASKTCTLDEAVELNGELPPIDVLAIRENDQVYYFAFGGCHRFQAYERKGPETLVKCKILPATKRQLKLYLGGSVDHIFKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA06</accession>
    <name>A0A1E3PA06_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">T-complex protein 1 subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_38392</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041456.1">
        <property type="nucleotide sequence ID" value="XM_019182536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA06"/>
    <dbReference type="STRING" id="683960.A0A1E3PA06"/>
    <dbReference type="GeneID" id="30199782"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03338">
        <property type="entry name" value="TCP1_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000010">
        <property type="entry name" value="T-complex protein 1 subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012717">
        <property type="entry name" value="Chap_CCT_delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02342">
        <property type="entry name" value="chap_CCT_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="529" mass="57148" checksum="BF0B5BE65A29077F" modified="2017-01-18" version="1">MAKAAVAPSNATFKNKEKPQEVRKSNILAARAVADAIRTSLGPKGMDKMIKTGKGEIIISNDGHTILQHMAVLHPAARMLVDVSGAQDVEAGDGTTSVVILTGALLGAAEKLLQKGIHPTIIAESFQRAAARSVEILLDMSTKISLDDREALIRAASTSLSSKIVSQHSHLLGPLAVDSVLKITSPDSSNVDLNDIRLIKKVGGTIDDTQLVDGVVLTQNVVKNAGGPTRIEKARIGLIQFQLSPPKPDMENNVVVNDYRQMDKILKEERAYLLNICKKIKKAKCNVLLIQKSILRDAVNDLSLHFLSKLGILVIKDIERDEIEFLSKSLSCKPISDVDLFTEDRLGSADLVEETESSGSKIVKITGVKNVTSPTVSVICRGANNLVLDEAERSLHDALCVVRCLVKEKALIAGGGAPEIEVSRTLMKEARSLSGTEAFIWNEFAEALEVIPTTLAENAGMNSINVVTDLRNRHENGERNAGISVRRSAATNTYDEHVLQPVLVSTSAITLASETVKSILRIDDITFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAB6</accession>
    <name>A0A1E3PAB6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SPIN90/Ldb17 leucine-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_25696</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041447.1">
        <property type="nucleotide sequence ID" value="XM_019181176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAB6"/>
    <dbReference type="STRING" id="683960.A0A1E3PAB6"/>
    <dbReference type="GeneID" id="30198422"/>
    <dbReference type="OrthoDB" id="445362at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030125">
        <property type="entry name" value="SPIN90/Ldb17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018556">
        <property type="entry name" value="SPIN90/Ldb17_LRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13357:SF1">
        <property type="entry name" value="NCK-INTERACTING PROTEIN WITH SH3 DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13357">
        <property type="entry name" value="SH3 ADAPTER PROTEIN SPIN90 NCK INTERACTING PROTEIN WITH SH3 DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09431">
        <property type="entry name" value="SPIN90_LRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SPIN90/Ldb17 leucine-rich" evidence="2">
        <location>
            <begin position="201"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="394"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="432" mass="50447" checksum="37E49D80578ADBB1" modified="2017-01-18" version="1">METDLIFPNGDEFWKYIENTLSCENFDDYHIANRKLVTFIKTVADHVQIYITNDQDIYRCGVYLINSPIYEAHRIFCISKMLSFLNIDLLDQNFKLFVSYVLLLDCKNDASILQVVQDYQGFHVLYKNLHQNFENLAIFSNDEGGERQTIAVIKKICTVQLDLMFQMCKYLSISVEELSLIDTFFVNYIFESLVIIDADEDVFNFAKFKVILSINEQFMIVSRENKIENKVFQTIATHTTFRNFSECLLVYFNREEDRCLQIMISKVLYLIFTNKTTSELFYHNDLNVLVDVLLRELTDLSEEEESIRNTFLRVLYPILKCSQFENTLYKKDKLKSLLGYFAGQSESKFWHQTETTKRLALRCLSIKWLALEDDDSASNSPSPTKDTLTVPEVNNSSTSINSISKRQPPPAPLPRKCFGRNNSNQHIPQRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAB9</accession>
    <name>A0A1E3PAB9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_24927</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041099.1">
        <property type="nucleotide sequence ID" value="XM_019181075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAB9"/>
    <dbReference type="STRING" id="683960.A0A1E3PAB9"/>
    <dbReference type="GeneID" id="30198321"/>
    <dbReference type="OrthoDB" id="5215911at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF30">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04702)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="452"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="490"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="521"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="59"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="567" mass="63427" checksum="20407CF2BB8DEB0C" modified="2017-01-18" version="1">MGHGAINYKAVPGTTHLVDLDGHMNAHHSKGDSQIVLIPTPSEDPDDPLNWSARRKWLAVFCNVVYTFGVGVPSAAIYSVLTNIAAETDITLGQLNAGTGYMFLFFGLGCLIFQPLALQYGKRPTYLLSMFATTLICVWSPYCKSNGEWIGSKILQGFFGAPIESLGEITMSDIFFEHERAKGMGIYAVALLTSNFLAPMIAGFISDGIGWKWVMWMCSIFGAVCFVFLFFFMEETNYNRKLGSKKINIMTIEGIGKDEENITSVTSEEKRIGTVLSHQTMEERMQETNENLYNEVDMASSSDELNYESKPRKTFKDKLSLTGGFKEKFLLLHYFIGPFRMARFPSVLWGGFLYGSSLVWFNLLNATEATVLGGAPYHFSSAMCGLAYISPTIFSVVFFFLSGYLSDFLKVKLAKRRNGLSLPEDRFWVLIVYMVLGFLASIGWGVGAHYGAHWMVLVVSMGVLGGLGVFGCISAVTYCSDAYHELDCEAMVVVILIRNLMSFACSYGLTDWVVNMGYKNAFISSGCILFFCNGSFLLMRYTGPFWRNKTKHIYWKYVEQNRKVVGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAG3</accession>
    <name>A0A1E3PAG3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent RNA helicase DBP10</fullName>
            <ecNumber evidence="4">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP-dependent RNA helicase dbp10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">WICANDRAFT_49892</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits and is required for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family. DDX54/DBP10 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="4"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041600.1">
        <property type="nucleotide sequence ID" value="XM_019182641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAG3"/>
    <dbReference type="STRING" id="683960.A0A1E3PAG3"/>
    <dbReference type="GeneID" id="30199887"/>
    <dbReference type="OrthoDB" id="10261375at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17959">
        <property type="entry name" value="DEADc_DDX54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ATP-dependent RNA helicase DDX54"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012541">
        <property type="entry name" value="DBP10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033517">
        <property type="entry name" value="DDX54/DBP10_DEAD-box_helicase"/>
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        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
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        <property type="entry name" value="DEAD_box_RNA_helicase"/>
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        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
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        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DBP10CT"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DBP10CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0347">Helicase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="20">
        <location>
            <begin position="106"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="137"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="357"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="361"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="831"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="106"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="366"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="379"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="831"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="866"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="905"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="920"/>
            <end position="932"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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            <dbReference type="ARBA" id="ARBA00010379"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00019117"/>
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            <dbReference type="ARBA" id="ARBA00021760"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022741"/>
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            <dbReference type="ARBA" id="ARBA00022801"/>
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            <dbReference type="ARBA" id="ARBA00022806"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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            <dbReference type="ARBA" id="ARBA00022884"/>
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        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ62393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="932" mass="105159" checksum="A02D0DCCACE4DFE6" modified="2017-01-18" version="1">MSSSDEEDFDIAGNIALDGSESEAYSSDDNSDVEVQDEIISSDDDEDEQQAPAKRQKVAPQQKKKDTAFPTLELSDDEGELEKKKEDVEFSSYFNIKSEATKAKAGSFASFGLSKFVLSNISKKGFRQPTPIQRKTIPLILQNRDIVGMARTGSGKTAAFSLPLVEKLKIHSAKVGARALVLSPSRELAIQTYKVIKEFSKGSDLRTVLLIGGDSLEDQFSLMMSNPDIIIATPGRFLHLKVEMNLDLKTIEYVVFDEADRLFEMGFEEQLNELLSALPPNRQSLLFSATLPRSLVDFAKAGLTNPVLVRLDAESKISDQLEMCFVSTKNNEREGNLLNLIQDVIKIPLATQDEIKKLDSLNKETDSEDSDNEDEDKSKKRKKNFKRKRLPPANQLPSPHSTIVFVPTRHHVEYITTMLKDSGYAVSFIYGTLDQHARKRQLLNFRAGLAPILVVTDVAARGIDIPVLANVINYSLPGSSKIFVHRVGRTARAGNKGWAYSIVSENELPYLLDLELFLGKKIMLTPMFEKKCEILKEQQGDSYVEPKVSYTERMVLGAAPRMNVENMSELFTNLINNDYDLKVLQGVAAKAEKLYFRTRTSASTESVKRAKEVKIGGWDEQNLLFGKNLEKEKLDFLAKLQNRNHKETVFEFGKRDDSLVDLMNTRRRQIAPIQRRAKERKELLEMERIAGLTHGLEDEILKGDSNEVGYSVNDKVLDDEFETAEELLNSKKPKKSFRDPNFYMSHYAPSAVIQDQQLSLNSGFTNDAQNATFDLNNDDNIQTHKQTQTMQWDKKAGKYVNSKSTDKKYIIGESGQKLPASFRSGRYDAWKKGKKTDGVKVGARESANDGGGGKKFNHKKQQAPRAPDKKRDDYHKQKKKVEQALEKGIHVKGFNKAGKGSNEMKSTEQIRKQRELQEKRRAKNARPSKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAK1</accession>
    <name>A0A1E3PAK1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP phosphoribosyltransferase</fullName>
            <ecNumber evidence="5">2.4.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_76092</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of the enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + ATP</text>
            <dbReference type="Rhea" id="RHEA:18473"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="2.4.2.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ATP phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.17" evidence="5"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041114.1">
        <property type="nucleotide sequence ID" value="XM_019184321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAK1"/>
    <dbReference type="STRING" id="683960.A0A1E3PAK1"/>
    <dbReference type="GeneID" id="30201567"/>
    <dbReference type="OrthoDB" id="2574at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003879">
        <property type="term" value="F:ATP phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd13592">
        <property type="entry name" value="PBP2_HisGL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.120:FF:000003">
        <property type="entry name" value="ATP phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000123">
        <property type="entry name" value="HIS1p ATP phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00079">
        <property type="entry name" value="HisG_Long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020621">
        <property type="entry name" value="ATP-PRT_HisG_long"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013820">
        <property type="entry name" value="ATP_PRibTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018198">
        <property type="entry name" value="ATP_PRibTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001348">
        <property type="entry name" value="ATP_PRibTrfase_HisG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013115">
        <property type="entry name" value="HisG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00070">
        <property type="entry name" value="hisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03455">
        <property type="entry name" value="HisG_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403:SF8">
        <property type="entry name" value="ATP PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403">
        <property type="entry name" value="ATP PHOSPHORIBOSYLTRANSFERASE ATP-PRTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01634">
        <property type="entry name" value="HisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08029">
        <property type="entry name" value="HisG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01316">
        <property type="entry name" value="ATP_P_PHORIBOSYLTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP phosphoribosyltransferase catalytic" evidence="15">
        <location>
            <begin position="59"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine biosynthesis HisG C-terminal" evidence="16">
        <location>
            <begin position="222"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000915"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004667"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009372"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ61907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="297" mass="32351" checksum="9ABAAD4AE6B9B95C" modified="2017-01-18" version="1">MDLVNHLTDRLLFAVPKKGRLYEKAIQLLKGSDIEFHRYNRLDIALSTNLPIALIFLPAADIPTFVGEGRCSLGITGVDQVEESEVDVELALDLTFGKCRLQVQVPQDGPIKDPKQLIGKTIVSSFTTLTRKYFEKLEGVKPGEPLKTKIKFVGGSVEASCALGVADAIVDLVESGETMRAAGLIDIDTVLSTSAYLIESKNPSHPELVKTIKNRIEGVLAAQKYILCNYNAPRNNLDALLSITPGRRAATVSPLDDEGWVAISSMIEKNKKGEIMDKLKAGGASDILLFEISNCRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAR2</accession>
    <name>A0A1E3PAR2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_76673</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041713.1">
        <property type="nucleotide sequence ID" value="XM_019184432.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAR2"/>
    <dbReference type="STRING" id="683960.A0A1E3PAR2"/>
    <dbReference type="GeneID" id="30201678"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005823">
        <property type="term" value="C:central plaque of spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990819">
        <property type="term" value="C:mating projection actin fusion focus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045160">
        <property type="term" value="C:myosin I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051019">
        <property type="term" value="F:mitogen-activated protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032120">
        <property type="term" value="P:ascospore-type prospore membrane formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007114">
        <property type="term" value="P:cell budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000742">
        <property type="term" value="P:karyogamy involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016237">
        <property type="term" value="P:microautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903525">
        <property type="term" value="P:regulation of membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051300">
        <property type="term" value="P:spindle pole body organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000527">
        <property type="entry name" value="Calmodulin-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="9"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="45"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="82"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="148" mass="16460" checksum="E6AA5EB00BF99560" modified="2017-01-18" version="1">MASEKLTEEQIAEFKEAFALFDKDQDGRISGTELSTVMRSLGFCPSESEVTDMMNEIDVNGDHQIEFSEFLALMSRQLKTNDSEQELLEAFKVFDKNGDGVISAAELKHVLTSIGEKLTDKEVDQMINEISDGSGEIDIKQFAALLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAZ3</accession>
    <name>A0A1E3PAZ3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_99004</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041768.1">
        <property type="nucleotide sequence ID" value="XM_019186674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAZ3"/>
    <dbReference type="STRING" id="683960.A0A1E3PAZ3"/>
    <dbReference type="GeneID" id="30203920"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000001">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004762">
        <property type="entry name" value="Amino_acid_permease_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00913">
        <property type="entry name" value="2A0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF10">
        <property type="entry name" value="S-ADENOSYLMETHIONINE PERMEASE SAM3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="390"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="414"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="460"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="496"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="7">
        <location>
            <begin position="64"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="570" mass="62409" checksum="72B3190EC4E7D983" modified="2017-01-18" version="1">MDSLEKGHSSNNSTSNLSAFESFKHSFKRRTVSIPPEVDQMDPYHKVNYILANQPLKKSLKRRHLQMIAIDGTLGTGLFIGLGYSLSQGPGATLIGFSIVGFGIYCVMQAAAEMAVAYPVSGSFASHVGRFVDPALGFTIASNYGLSWLISFPSELIGCSMTIKYWSDVNPVAWVAIFWTFIVTLNLFGVKGYGESEFILSIIKVLAIIIFIIIGIVLICGGGPNSTGYIGAKYWHDPGSFSHPALKSICSTLISAGFSYGGTELTLISAIETSKASSVTRATKQVFWRIALFYITTIVVIGCLVPRTDPRLLGGSTSQDITASPFVIALSNTGSMGVKVSHFMNAVILVAVLSVGNSCVYASSRVIQSLGASGQLPSIFAYIDNEGRPLVGIIVSALIGLLCFVVASSKQSIVFTWLFSLCSISAFFTWFSICLSHIRFRLALYKQGRNTEELGYKATLGIYGSIIGGIILLLVIIGEIWISIFPLGSPASVQTFFQNCLSIPLMIVVWSCYRIIKGTFFKKFWIPLDEIDLDTCKRYPDVDLLKQEQQLHAEKLAAKPLYYRIYRFWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBF1</accession>
    <name>A0A1E3PBF1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Activator of Hsp90 ATPase AHSA1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_82694</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041943.1">
        <property type="nucleotide sequence ID" value="XM_019185424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBF1"/>
    <dbReference type="STRING" id="683960.A0A1E3PBF1"/>
    <dbReference type="GeneID" id="30202670"/>
    <dbReference type="OrthoDB" id="567237at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08892">
        <property type="entry name" value="SRPBCC_Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.20">
        <property type="entry name" value="Activator of Hsp90 ATPase Aha1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036338">
        <property type="entry name" value="Aha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015310">
        <property type="entry name" value="AHSA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="ASHA1/2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 HSP90 CO-CHAPERONE AHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009:SF22">
        <property type="entry name" value="LD43819P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09229">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01000">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103111">
        <property type="entry name" value="Activator of Hsp90 ATPase, Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Activator of Hsp90 ATPase AHSA1-like N-terminal" evidence="3">
        <location>
            <begin position="13"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="162"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="340" mass="37825" checksum="812BE49280BE2AD6" modified="2017-01-18" version="1">MVVHNPNNWHYVEKNCIDWTRQYFKEKLVDLEAESDDKSQHAKITGVTSVEGDSIVAQKRGKVISLFDLKLALAYSGNVGETSVKGSITIPEVAYDSDEDDYQFEVSVFSETSENTKIKEVVKKKLIPQLRKAFAQYGKDLLSTHASDIQLPADQVKSELTKANQLKSSSSSTSVDKKQTETSNSTTSSSQPTPTQSSSSVPKYNTSTVHLEPVFNTTAEQLYVTFLEKNRVGAWTRANPGFNGDFLKVGDEFTLFGGNVSGKVTKLVPNETIEQQWRLNSWKEGHYANLTIGLHQGDSETKLDVLFKGIPIGEEDQVRGNFENYYVRAIKITFGFGAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBL5</accession>
    <name>A0A1E3PBL5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_60856</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042016.1">
        <property type="nucleotide sequence ID" value="XM_019183069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBL5"/>
    <dbReference type="STRING" id="683960.A0A1E3PBL5"/>
    <dbReference type="GeneID" id="30200315"/>
    <dbReference type="OrthoDB" id="10033309at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017771">
        <property type="entry name" value="Cyanamide_hydratase_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03401">
        <property type="entry name" value="cyanamide_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35569">
        <property type="entry name" value="CYANAMIDE HYDRATASE DDI2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35569:SF1">
        <property type="entry name" value="CYANAMIDE HYDRATASE DDI2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
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            <end position="162"/>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <sequence length="228" mass="25854" checksum="ED083BDEE768C2CF" modified="2017-01-18" version="1">MSKYGFVKIERDFIQSIPDANEPIVHHDTSLPQTELARKVLQFAKSQLSTPTLNHSLRVYLYSKAIIKDQFPDWDLDEEVIFVTALLHDLGTTDENLKATKMSFEYFGAIKARELVLKETNGDQDYADAVCEAIIRHQDLNDKGYITQLGLIIQISTVLDNAGFDSVSSLIHSDTLKVVNQRYNRDNWLNCFAGVVQKENVLKPWGHTSSLGVEEFPANVLKNPLHYT</sequence>
</entry>
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        <name type="synonym">Hansenula anomala</name>
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        <lineage>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:subtelomeric heterochromatin formation"/>
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    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        </location>
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            <end position="212"/>
        </location>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="212" mass="24455" checksum="674528C1A3932D2D" modified="2017-01-18" version="1">MPPATNKRIKTLSISRPIIYGNTAQPLGDKRPPGAPEEHTHSWTVFVKGPNGEDLSYFIKKVVFKLHDTYPSPTRSIESPPFQVTETGWGEFEIGIKIYFVNEANEKNISLYHHLRLHPYGLPPDAVLTDADRNVKSIQYDEIVFNEPTEQLFEIMTQKPGNRLPETKKQSEFSKETEREEIVRFEHGIKEVNQEIEKLKSQIQELQQLKAK</sequence>
</entry>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="GeneID" id="30201645"/>
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    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BED-type" evidence="6">
        <location>
            <begin position="160"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="46"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="121"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="194"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="374"/>
            <end position="413"/>
        </location>
    </feature>
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        <location>
            <begin position="59"/>
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        </location>
    </feature>
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            <end position="140"/>
        </location>
    </feature>
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        <location>
            <begin position="245"/>
            <end position="278"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="376"/>
            <end position="387"/>
        </location>
    </feature>
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        <location>
            <begin position="388"/>
            <end position="399"/>
        </location>
    </feature>
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        <location>
            <begin position="402"/>
            <end position="413"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
    <sequence length="413" mass="45956" checksum="040F9D9539B98BC1" modified="2017-01-18" version="1">MSNSINPLINFDRPQEHAIDSALVQALDSYEAAQVPHHLQQSHNLGRQQVHPHQHHQQLHQQNNGQLQHKQHDQSNDQRLLQQVPIDPSINDSTSEKNAAVAAAAAAAAAANDMLNARNTAQAAVNSSSKMNNSRNNSASTGPKITKPRINKPGQKFGAKKKSWVWNWFIQDTVDSNVATCDFCGKVIKRLASDKGSPKKLGEHLKTHKIDKNSINPKRDSMIVGDANRHYFNTLNAAQTFKPHPLSNQPSSSNSQSQQSHSQQSQQQNNHQTLSQNSPYSRQQTTGASNTNAEFTNTNYDRSGFDQNPYSQPKFQKDIMKFLVENKLPLAIVKSPSFRQVIFTLKPEAIPDLNELNHLYSSLLEVIHNEKGPLSNVDDVNNDQQNDSADHQTSRHDDSNVDDSLTASTSGWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="4">Low temperature requirement protein A</fullName>
        </recommendedName>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycotina</taxon>
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            <taxon>Phaffomycetales</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ62724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_019180689.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3PBX9"/>
    <dbReference type="GeneID" id="30197935"/>
    <dbReference type="OrthoDB" id="191995at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="Low_temperature_requirement_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36840">
        <property type="entry name" value="BLL5714 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="LtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="307"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="49466" checksum="0BE2F1FDDBB8C271" modified="2017-01-18" version="1" fragment="single">DVEEFIKKYGDINVSYIEPPPSSRWFTKPYALNYFHDGKLFRTKHERTSGRLELFLDLIYVGIAMNLSSNAIKEPNWLSFVKYLFYFIPAWTIWADLKDFMNYYFNDDLLQKFYVVFILALLLIYDNNVEFIFESRNALLTTITAYFLARITLAIMLIFYSIYVFEHRTQMRLYSATLIVTSSCWYFLLLIRSTAGIIVFSAIWLILEQCCFVISYHPWTKKKLGLTHSTALNIEHEDERLSAFFIISIGEFLLGIVSGNALSEGWNTKVAKGIALLIDAFIFLELYSHKDGSIRATHAMRRSVSTALGYIYIHLPLIASLLIVGDAGADLAKLGEPYEEERGVVFFFSAGIFIALCALTIHPLLDKPKDDPKDHLVNRYLRIGFRIPVGGIILGLAWTEWKIVTLIWIDTLFLMLLFVYELFAINPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCH6</accession>
    <name>A0A1E3PCH6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histone H1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_103356</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the histone H1/H5 family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ62587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="30197623"/>
    <dbReference type="OrthoDB" id="1110759at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:nucleosomal DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045910">
        <property type="term" value="P:negative regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="H1/H5"/>
    </dbReference>
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        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467:SF36">
        <property type="entry name" value="HISTONE 24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467">
        <property type="entry name" value="HISTONE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="9">
        <location>
            <begin position="22"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="83"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="127"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="144"/>
            <end position="179"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ62587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="187" mass="19679" checksum="1DDA37160359A2DD" modified="2017-01-18" version="1">MAKAASTTKATKATKASSAEKTHTPYAEMIKNGILNLKDRNGSSRQALKKYIQNNYKITASNFDSLFNAALRRGVEKEIFIQPKGTSGPVKLNKKKPAAEPKAVEAKAAAPAKKATTTKKTTTAKKPVAKKAAPKKAAAKKVTKAAPAKKPTPAKKAAAPAKKATAAKKAAPKKATTTKTTKRTAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCN7</accession>
    <name>A0A1E3PCN7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Subtelomeric hrmA-associated cluster protein AFUB-079030/YDR124W-like helical bundle domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_76900</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041939.1">
        <property type="nucleotide sequence ID" value="XM_019184463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCN7"/>
    <dbReference type="STRING" id="683960.A0A1E3PCN7"/>
    <dbReference type="GeneID" id="30201709"/>
    <dbReference type="OrthoDB" id="5338458at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047092">
        <property type="entry name" value="AFUB_07903/YDR124W-like_hel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021264">
        <property type="entry name" value="AFUB_079030/YDR124W-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023278">
        <property type="entry name" value="Ethylene_insens-like_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36102">
        <property type="entry name" value="CHROMOSOME 10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36102:SF1">
        <property type="entry name" value="YDR124W-LIKE HELICAL BUNDLE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11001">
        <property type="entry name" value="AFUB_07903_YDR124W_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116768">
        <property type="entry name" value="DNA-binding domain of EIN3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Subtelomeric hrmA-associated cluster protein AFUB-079030/YDR124W-like helical bundle" evidence="2">
        <location>
            <begin position="97"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="417" mass="47636" checksum="07595E8F2DFD0F93" modified="2017-01-18" version="1">MNQLEDEIIEALTKFVQEDGSFALLLSFPNGLVKFHSSDDFKSYIDLFTQDQTDPANSQSPASLNNNVDTPKQETQEKGLIPLVRALESPTFELHLSSKREVMNHLRACFREMQQLACKTIAKAWIKAIEPKKQTNFPYNRGNDSRPPWWPSHIRHCEPDHLVKSERIDLLVGIARHEDTDVRKLRDATNRVPSLDKHRLKVLDEVYLLVKTEKEEGEGTLLVSDFENATRLSTKKRKPVRAVPFRRRYVKKVELEDDDYDQADETNDQVEEQEKQESQIAEDTTLTEDEVPVEEEVQKENDIAGVSSVTDPPPSRIFDAPPSAFENPGASSSLPDEASTTPPPPKRYTFRDFVKTTPIGTTTTEDGRVFPNAFNLQMFSASRVDPEVLEQSSRYPAYAQSPQQDEDEFEQDDHIWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTX5</accession>
    <name>A0A1E3NTX5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_65905</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454245">
        <property type="protein sequence ID" value="ODQ56629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035836.1">
        <property type="nucleotide sequence ID" value="XM_019184018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTX5"/>
    <dbReference type="STRING" id="683960.A0A1E3NTX5"/>
    <dbReference type="GeneID" id="30201264"/>
    <dbReference type="OrthoDB" id="3980873at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="127"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="16"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="61"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ56629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="274" mass="31224" checksum="2A777686D32A42BF" modified="2017-01-18" version="1" fragment="single">MNSYIQNYGDQPLDLSTAPSSSSNVSSYFMNQHHSQSSNNGNNNNNGISNQFWRKPNTANTTSSSTFTPITSANNNNNNTANSSTSTIPPQYSNQQRSFNNDRHFNQIPANNNNNNFDYPSNNNEKEERLKIDLKIKESQIESLENEIQRLSESMNLIDSKNNNSLLSNNQQIPKNINEIFNKLTNNYTILENELNDTKKRLESLITSITLNPTNSITNNGRYDELEISHKIILKLDLLKQANEEMLKQLSFGKSKQVDIELNLLKMENAKLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU40</accession>
    <name>A0A1E3NU40_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome alpha-type subunits domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_98189</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454216">
        <property type="protein sequence ID" value="ODQ56701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035908.1">
        <property type="nucleotide sequence ID" value="XM_019186640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU40"/>
    <dbReference type="STRING" id="683960.A0A1E3NU40"/>
    <dbReference type="GeneID" id="30203886"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080129">
        <property type="term" value="P:proteasome core complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ56701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="19387" checksum="DD39D5DC9ECDC960" modified="2017-01-18" version="1">MTADAGILINSARIQAQNYLKTYNEEIPVELLVRRLCDIKQGYTQHGGLRPFGVSFIYAGYDERYDYQLYTSNPSGNYSGWKATSIGANNAAAQTLLKQDYKNDITLNQASELALKVLSKTMDTTTLSSEKIEFATIQKDKNGKIYQRLWKPAEIEALLNETGITKKEDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUQ3</accession>
    <name>A0A1E3NUQ3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_65694</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036020.1">
        <property type="nucleotide sequence ID" value="XM_019183969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUQ3"/>
    <dbReference type="STRING" id="683960.A0A1E3NUQ3"/>
    <dbReference type="GeneID" id="30201215"/>
    <dbReference type="OrthoDB" id="1405469at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000306">
        <property type="entry name" value="IKS protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF138">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE IKS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="142"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ56813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="504" mass="57156" checksum="65AF105A631919D6" modified="2017-01-18" version="1">MNSLLSNVHNLTPGFVRNDPLRNAVVLHNKITNELSVVSTISPPVDHELIPYQGESASPVIEIGRMRKCPHCGRVFEEGSASSTSSQTGNNNNAKAFDAGTDDSFIDSNYFRLLGNSSISGEGATNNLPDSLFTQGYFNKFFKEIELLGRGARGSVYKVEHILNGVSLGFFAMKKITIGDDSKWLLKVLKEVTMLCKISFNNNNLVNYNHVWLEISSINDFGPKIPCAYILQQYCSGGNLEEFIINLQNPILKPHELKKWKLSHKRQQLKGRLLNNDEILLIFQQIVSGVYELHKNHIIHRDLKPSNCLLAEPYFTDFTQLESDISKIPTILVSDFGESQLEGEFRNATGATGTLEYTAPELLNFNDDNVPQFNKKTDVYALGMILYFMCFGKLPYEYSNEVELLKNEIKGSNELMMIDREDLLPELKYLIKNLTSKNPDERMSVSEILDVLARFDHLEKNEKEEEKIDNPQPLRRLSLTDAPIKITCWPIEFVNFELYPSPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUQ9</accession>
    <name>A0A1E3NUQ9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">4-aminobutyrate aminotransferase</fullName>
            <ecNumber evidence="4">2.6.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GABA aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Gamma-amino-N-butyrate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_36732</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23352"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="2.6.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer and homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.19" evidence="4"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036126.1">
        <property type="nucleotide sequence ID" value="XM_019182465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUQ9"/>
    <dbReference type="STRING" id="683960.A0A1E3NUQ9"/>
    <dbReference type="GeneID" id="30199711"/>
    <dbReference type="OrthoDB" id="10260828at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034386">
        <property type="term" value="F:4-aminobutyrate:2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000029">
        <property type="entry name" value="4-aminobutyrate aminotransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004631">
        <property type="entry name" value="4NH2But_aminotransferase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00699">
        <property type="entry name" value="GABAtrns_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43206:SF1">
        <property type="entry name" value="4-AMINOBUTYRATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43206">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ56919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="470" mass="51766" checksum="EA369A539D57307A" modified="2017-01-18" version="1">MSVAAKYYPNEPTEPVIKTAEIPGPASKAGIESLGKVFDSRPVYFIADYEKSLGNFIADVDGNVYLDTYAQIASVPLGYNNPALIEAAKSPEMLRAIIERPALGNFPGKDLEGIIKNVLKVAPEGQDQVWNGLSGADANELAFKAAFMWYQQQKRGNADFTTEENESTMENQAPGSPDLAVLSFKRAFHGRLFASASVTASKPVHKLDLPSFKWPKAEFPSYQYPLEDHVEENKAEDARTLAIVENLIKTWSVPIAALIIEPIQSEGGDNHASAEFFQGLRDITLKHGVVFIVDEVQTGLGATGKLWAHEHFKISPAPDLVTFSKKFQSAGYFFHDPKFVPNVAYRQFNTWCGDPARMILAGAIGKEVLDQGLVAKAAEVGDYLYSELEKVAAKYPTKLQNLRGKNRATFIAWDLESGAARNAFLYKMKQNGVNIGGCAEVSVRLRPTLTFEKKHADLFVKVLNKTLSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUS5</accession>
    <name>A0A1E3NUS5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA-splicing endonuclease subunit SEN34</fullName>
            <ecNumber evidence="2">4.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11 12">tRNA-intron endonuclease SEN34</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA-splicing endonuclease subunit Sen34</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_15917</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.</text>
            <dbReference type="EC" id="4.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN15.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA-intron endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.16" evidence="2"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036136.1">
        <property type="nucleotide sequence ID" value="XM_019180665.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUS5"/>
    <dbReference type="STRING" id="683960.A0A1E3NUS5"/>
    <dbReference type="GeneID" id="30197911"/>
    <dbReference type="OrthoDB" id="48041at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000214">
        <property type="term" value="C:tRNA-intron endonuclease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000379">
        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22363">
        <property type="entry name" value="tRNA-intron_lyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1350.10:FF:000008">
        <property type="entry name" value="tRNA-splicing endonuclease subunit Sen34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016690">
        <property type="entry name" value="TSEN34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059049">
        <property type="entry name" value="TSEN34_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13070:SF0">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13070">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26577">
        <property type="entry name" value="TSEN34_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017250">
        <property type="entry name" value="tRNA_splic_SEN34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TSEN34 N-terminal" evidence="15">
        <location>
            <begin position="2"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA intron endonuclease catalytic" evidence="14">
        <location>
            <begin position="166"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR017250-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF26577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ56929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="264" mass="29656" checksum="17832AC41D6EAA72" modified="2017-01-18" version="1" fragment="single">AGKALVFNLDVIKSLRSLGIGGVLSGTLPTAPQQNIFLGIPLQLMVEEVIWLVTKGYAYLIPDEKLIKELVDDLDEEDIKSIQYERAIGFQSQKEQKLNQLNDKLNSLKKKVQKTPPSDALITQSLFVNIYDDSNSLPNNTLLKSIYAKDIKLQKRLLESLNYDRLNYLIFDYLKSKEYFLAPGLRFGGKFIGYPGDPLRYHAHLIINAVGWDQDIGLMNIVNGGRLATGVKKVWLIGSENNDMDIDKDDGEVVCFSVEWAGFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV44</accession>
    <name>A0A1E3NV44_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_65217</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036161.1">
        <property type="nucleotide sequence ID" value="XM_019183873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV44"/>
    <dbReference type="STRING" id="683960.A0A1E3NV44"/>
    <dbReference type="GeneID" id="30201119"/>
    <dbReference type="OrthoDB" id="4085284at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="3"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ56954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="96" mass="11012" checksum="9433083F973DB813" modified="2017-01-18" version="1">MSSSIRVSNLPKDVTEETIHSLFLSFGNILSITSQKNYIDIQFQEISDAQAALDNMTGFKLFDSYLQVSKISEYNPLDSLEEKKPIWEQNHVLNQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV85</accession>
    <name>A0A1E3NV85_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_85919</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036147.1">
        <property type="nucleotide sequence ID" value="XM_019185971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV85"/>
    <dbReference type="STRING" id="683960.A0A1E3NV85"/>
    <dbReference type="GeneID" id="30203217"/>
    <dbReference type="OrthoDB" id="3064516at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF8">
        <property type="entry name" value="PEPTIDASE M20 DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133518" description="Peptidase M20 dimerisation domain-containing protein" evidence="6">
        <location>
            <begin position="18"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="7">
        <location>
            <begin position="202"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ56940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="390" mass="43139" checksum="156E415404200202" modified="2017-01-18" version="1">MLFASLIVTCFSLLAAGSPLQFPLLSELYQYSPDHDLSKPKIDQLFELHKLLVQIPSVSYSEYEVSTKLADYLRSHQLSVDLQRVAENRYNVFAYKGYQNDTKVLLTSHIDTVPPFFPYKISKYGKRIYGRGSTDAKGSVAAQVISFLDLLENGDIKEGDLSLLFVVGEENGGHGMQYVNSNIDYHWETVIFGEPTENKLGVGHKGGYGGVLNVTGLASHSGYPELGINANTILVEFLSTLLSQKLPFDDLLGNSSLNIGEINGGVAANVIPDHAQAILSFRVASHLKFFQDLIPGLIKESKYRDRIEFFSTIGKGRGPVFFDYKIDGFESIVLGYGTDAFSLSRKQLKNKILYGPGSIHVAHGANEFVSTDDLIKAVEDYKKLVKYGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVH2</accession>
    <name>A0A1E3NVH2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SPX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_86050</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036333.1">
        <property type="nucleotide sequence ID" value="XM_019185986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVH2"/>
    <dbReference type="STRING" id="683960.A0A1E3NVH2"/>
    <dbReference type="GeneID" id="30203232"/>
    <dbReference type="OrthoDB" id="10260443at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990816">
        <property type="term" value="C:vacuole-mitochondrion membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015169">
        <property type="term" value="F:glycerol-3-phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006797">
        <property type="term" value="P:polyphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000185">
        <property type="term" value="P:regulation of phosphate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01115">
        <property type="entry name" value="SLC13_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14478">
        <property type="entry name" value="SPX_PHO87_PHO90_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF92">
        <property type="entry name" value="LOW-AFFINITY PHOSPHATE TRANSPORTER PHO91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="358"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="387"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="437"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="483"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="524"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="567"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="610"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="641"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="664"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="707"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="800"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="8">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="819" mass="90589" checksum="0238317A1C3B641F" modified="2017-01-18" version="1">MKFSHSLQFNAVPEWSSKYIAYSNLKKLIYNLQRESLTSRKINANDTEASYLISHDNQHQERDLSKVFLAALDSELKKIDSFYKEKEEVTYADFETLCKDLDDFENEIRTTQSNESNTAANTHKQPYRAKSIPNDFNEQIGDDSGVIDSDDPNYVQPYRGRSGSLQLSQRARKNSDLVRSRSPEFWDTDYDNLAFGNDPFSVLADIRITLKRRSIALFTVLSELKSFIELNKTGFTKALKKFDKSLNTKLKADYLENLDNNSYVFRSDTANVLKDKIDRLVQIYASLATGGNIENAKSELRLHLREHVVWERNTVWKDMIGLERKAQNATVAKQTDSKEVLVGEEGAPISSKRLLNPTAWKLLFIIAVTLLLMIFSPFKQVEQKNCFALLVCSSLLWATETIPLFATSLLVPLLIVVLGVLKDDQGERLDPEASSKFIFSAMWSSVIMLLLGGFTLAAALSKYNIAKILSTWILSKAGTNPKVILLTIMSVALVVSMWVSNVAAPVLCYSIIQSLLRTLPRGSVFAKQLILGIALASNIGGMASPIASPQNIISIGIMDPQPSWGQWFVISIPVCAISLILVWLFLLVSFKSNEQVSIAGIRTVDEKFTPTQWFISIVTIGTILLWCLAHQLEGVFGEMGIISIIPMLLFFGTGILSAEDFNNFLWTIVMLAMGGIALGKAVSSSGLLATIALAIQSRVEGFSLFSIMIIFGLLILVVATFVSHTVAALIIVPLIAEIGAQLDDPHPNLLILTSAFLCSCAMGLPTSGFPNVTAISLVDDFGERYLTVGNFISRGVPSSLIAYLVVVTVGFVLMRVLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVQ4</accession>
    <name>A0A1E3NVQ4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAM86 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_81605</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035979.1">
        <property type="nucleotide sequence ID" value="XM_019185268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVQ4"/>
    <dbReference type="STRING" id="683960.A0A1E3NVQ4"/>
    <dbReference type="GeneID" id="30202514"/>
    <dbReference type="OrthoDB" id="194386at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF130">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE EEF2KMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ56772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="32852" checksum="A657885B59153CB1" modified="2017-01-18" version="1">MDNLLNNLRQRIPIQEISIPKELSDDELLNVLTEISQENPYYVKSFFNKLFRTDIDQDEFYELFSEVINSKPKQVTETDIITYNINGVLVKIRETPRIISGSGTTGLRTWEAALYLSLYLSNHLELVQNGNVLELGTGTGLVSLYLLKEPKLKLQDLIITDGDSALIESLSHNFKLNDISIASVNCHSLWWGKDVVPSSIDTIIAADVTYDAEVIPSLVKCIQTGLEQGAKQALIAATIRNEDTIKVFEETIVQNGLNFTIESRVRKPSELDATVWFSQLTQEIRIYKITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVZ9</accession>
    <name>A0A1E3NVZ9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ADP/ATP translocase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">ADP,ATP carrier protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_36159</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ADP(in) + ATP(out) = ADP(out) + ATP(in)</text>
            <dbReference type="Rhea" id="RHEA:34999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:35000"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 16">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036501.1">
        <property type="nucleotide sequence ID" value="XM_019182400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVZ9"/>
    <dbReference type="STRING" id="683960.A0A1E3NVZ9"/>
    <dbReference type="GeneID" id="30199646"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009061">
        <property type="term" value="P:anaerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015886">
        <property type="term" value="P:heme transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000001">
        <property type="entry name" value="ADP,ATP carrier protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF14">
        <property type="entry name" value="ADP_ATP TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00927">
        <property type="entry name" value="ADPTRNSLCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="11 14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="7"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="111"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="211"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ57294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="303" mass="33030" checksum="D99058BF38D8B137" modified="2017-01-18" version="1">MSEQPKKSFLTDFLMGGVSAAVSKTAAAPIERVKLLIQNQDEMIKQGRLSHKYNGIAECFKRTAADEGIISFWRGNTANVIRYFPTQALNFAFKDKFKSMFGFKKSEGYWKWFAGNLASGGLAGGSSLLFVYSLDYARTRLANDAKSSKGSGERQFNGLVDVYRKTLASDGIAGLYRGFLPSVVGIVVYRGLYFGLYDSLKPVVLVGSLEGSFLASFLLGWVVTTGASTASYPLDTVRRRMMMTSGQKVHYSGAFDAFRKIVAAEGVKSLFNGCGANILRGIAGALVISLYDKLQVLLLGKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW00</accession>
    <name>A0A1E3NW00_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_65640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035964.1">
        <property type="nucleotide sequence ID" value="XM_019183957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW00"/>
    <dbReference type="GeneID" id="30201203"/>
    <dbReference type="OrthoDB" id="2564148at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ56757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="773" mass="90361" checksum="176CE6293F00BEB9" modified="2017-01-18" version="1">MSSPNVLSNSWKLGELVPPLHSPRALHHHHSSSNSPYNNSLLYNDNNNNNNFNYSFINKDFIDFDPHDQSTALPNHASISAFRNLTSTPRSSHPTSLPKSLPIDWNLFNKQFTLNQKLRQSKLKRKRSELGDATDVDVDDEPQIQNNFENLPFQVIDFIADCVESQTDLINLSQTCKFFNRIINKRLYSKIIIVDKITDKHKNYDSNYTLLDVKNYPKFLASLHSNEIIIQLINEIIIDTSSNNTSYDQLTDNIFESLYELFLTKNNNLKVLENLDLNNLKKFDSLIQYNRDYLIKHNHNKYNYYVSSADSLDEIIRKDLLNLQNFQLLDLNDINKLPSSTKSLSIFIENQHNFKDNFKLNLNGWRVLSNLTSLQLSTPTTFDSFVEELYSIYTNTNNTFKPTKLSNIKTLSISNVHKYDLENGHSNELSLAKLNTIIDMSKIQELELKVKCYENVLCNCCIMKFFNDWYETLGLDSKFSSLTKLSIINHSPSSNSNINIQWDQILTNKIFLEKFGGENLEFFFINLNDFPFVPLFEKTASFETNDSMKLPINNDILRIRKLMYQSLLKSFKNLQDFIIPDFFYNWLVYNNIEDLSNDDNLNYLNYLDSCHCVECCEARLIFKNYSKTTAFKDFKGRFNSNLNYNKGPCVSNYNKLNDSPDSYRIFYNFLIRELKSRIPLKQLNKIQNSLNMEKFPPNQISNTIVSKQDFNKLIKLIIHSLKHHINYILVQLPNIKKFNLGGILFKCYKQQDNKIMVHAVYDDYNEVFKIRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW86</accession>
    <name>A0A1E3NW86_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_65517</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036467.1">
        <property type="nucleotide sequence ID" value="XM_019183927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW86"/>
    <dbReference type="STRING" id="683960.A0A1E3NW86"/>
    <dbReference type="GeneID" id="30201173"/>
    <dbReference type="OrthoDB" id="440781at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021569">
        <property type="entry name" value="TUG-UBL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46467">
        <property type="entry name" value="TETHER CONTAINING UBX DOMAIN FOR GLUT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46467:SF1">
        <property type="entry name" value="TETHER CONTAINING UBX DOMAIN FOR GLUT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11470">
        <property type="entry name" value="TUG-UBL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="315"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="477" mass="53714" checksum="B770773D8276B607" modified="2017-01-18" version="1">MSSVAISYELRTIKLKATPGTPVSELLEKAAAHFKLDSATTGLFHNNSELSTSLPFRLLNLPSGAKLTLKATKTKKASEINIKVQFINSTFEKNAVVKKVGNQLTLGQLVQEIEKDILSPILSTSKNLKFQGMTKVVTQEDFGNVTLAQFGAENNASIRLIFEVSNKVTKPENKPTDEAAFIHEKAKEPEKERNVSSTILEEKPTQDVPDIESQDNTAQDKQDTPVALKPDDDDVNAMEVDEPQQQSQNKGAATEEIQKHLYVYKAPEKPIETYEPQEEDYDLTVSHARQYQSILSQRAHESYKSKRLRQEANKKKITNIEIRIRFPDESSLQVNFTPLETNKDLYNLVASTLVDTSSFVLSIPYPLKDISNDETKLTESFESRNLLLFKSDKTSGPFLKDEYLDKAKDLKEAEDVKLDLERQLMKDDNDEDNIDDSKIQNGQIPKKTESSSFKSKTPASEQKEGSKVPKWLKLGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW88</accession>
    <name>A0A1E3NW88_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein S2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_17924</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036590.1">
        <property type="nucleotide sequence ID" value="XM_019180765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW88"/>
    <dbReference type="STRING" id="683960.A0A1E3NW88"/>
    <dbReference type="GeneID" id="30198011"/>
    <dbReference type="OrthoDB" id="2320368at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000055">
        <property type="entry name" value="Mitochondrial ribosomal protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_B">
        <property type="entry name" value="Ribosomal_uS2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005706">
        <property type="entry name" value="Ribosomal_uS2_bac/mit/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01011">
        <property type="entry name" value="rpsB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S2 PROKARYOTIC AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="29573" checksum="D243B8725E7FA8E2" modified="2017-01-18" version="1" fragment="single">KFPFLIPSKKSEPYTSQELHLRREFFNSSNSKLGSNLTDVYIPHHEVFYPKSIKNVSISKLLAAGAHLGHSTSLFRPSTQPYILGSYKGIHIIDLEQTLSYLRTASKVVEGVAERGGIILFIGTRENLQRPLELAAERCGGYYVATRWVPGTITNCTEISSWDRQEVDFEGVATGRVLNADEVKGLVKPDLVVILNPVENRVAVRECIQSRIPTIGIIDTDSEPSLVSYPIPANDDSVRCLTLLVGVLSKAGETGRLKRFKKIQDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWB0</accession>
    <name>A0A1E3NWB0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aminopeptidase</fullName>
            <ecNumber evidence="11">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_64766</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="11"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036625.1">
        <property type="nucleotide sequence ID" value="XM_019183798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWB0"/>
    <dbReference type="STRING" id="683960.A0A1E3NWB0"/>
    <dbReference type="GeneID" id="30201044"/>
    <dbReference type="OrthoDB" id="10031169at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061957">
        <property type="term" value="C:NVT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120113">
        <property type="term" value="P:cytoplasm to vacuole targeting by the NVT pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000001">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000004">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF174">
        <property type="entry name" value="PUROMYCIN-SENSITIVE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="14">
        <location>
            <begin position="104"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="12">
        <location>
            <begin position="317"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="13">
        <location>
            <begin position="609"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="390"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ57418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="952" mass="107537" checksum="5711E7E0C48FC4DD" modified="2017-01-18" version="1">MSALTQKLASPISSAISRRSLKKLLDLKRFASTFVIKNPNSYSSNYNSFNLNSIVRNQARLPQGINYTHTRFHSYHSHSRMCQSAGGVATNIPSDREVLPTNVKPIKYDLTFDPNFETFKFDGNAKIDLDVLEESNTITLHTLDIEIHSTKVGEIKPTGTKFDEAKQSVTFTFKDNFTKGSKITIDIDFTGVLNNNMAGFYKSSYKENGETKYLATTQMEPTDCRRAFPSFDEPALKAIFDITLVCDNHLTALSNMDVKEVKQLDNGRKATVFNSTPLMSTYLVAFIVGELKYVESNDFRVPVRCYASPGLEKQCQYSADLTAKTLAFFEKAFDIKYPLPKLDNVAIADFSAGAMENFGLVTYRVAELVYDEENSTLATKQRISEVVQHELAHQWFGNLVTMEWWEGLWLNEGFATWMSWYSCNEFYPDWKVWESYVSDSLQQALNLDALRSSHPVEVTVKRADEINQIFDAISYAKGSSLLKMISGWLGEDVFIKGVSNYLKKHQFGNTQTEDLWEALSEASGKDVVKVMGIWTQKTGYPIISVEEDAENGTIKVKQNRYLSTGDVKPEEDEVIFPVFLALRTQDGIDKSVVLDSREKTLKLEKGLDFYKLNGDQFGYYRTSYPPERWAKLGKAGSEGLLSVEDRTGLVADAGALATSGYQSTSNLLSLVHSWKNETNYVVWTEIITRVAAVKNAWIFEDQAVTDALKQFSKSLVAPTIHSLGWEFKKSDSFKDQNLKTVLYGSASASGDEIITKHSLEIFKKYVEGDKKAIHPNIKPSVFATVARNGSTKEYEQILDIYNNPISPDEKIMALRTLGRFEEPELIQRTLGLLFDENTIKNQDIYVPMQGLRTHKAGIEALWKWVQLNWDDIIKVLPPGFSLLGSVVKISTSAFSSDEKIEEIQKFFATKDTKGFDQGLAQSLDTIRSKSQWIKRDSEDVNAWLTEQGFLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWM5</accession>
    <name>A0A1E3NWM5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_85714</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036788.1">
        <property type="nucleotide sequence ID" value="XM_019185936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWM5"/>
    <dbReference type="STRING" id="683960.A0A1E3NWM5"/>
    <dbReference type="GeneID" id="30203182"/>
    <dbReference type="OrthoDB" id="1888931at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050085">
        <property type="term" value="F:mannitol 2-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000090">
        <property type="entry name" value="NADP-dependent mannitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF13">
        <property type="entry name" value="L-XYLULOSE REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="292" mass="31909" checksum="95387CD4A8AEFF4D" modified="2017-01-18" version="1">MTIPVRQEGAEKIVSYISSEFSDELPRPNPERKHVSDLFKLTDKVAVVTGGARGIGFAIADCFAEADAKVVVLIDYSNDVVEVAQELSKKNKGSKIVGYRCDVSKSEQVEETINSIESEFGTIDVFVANAGVVWKTGNVINKENDDYSSWNQLMDVNLNGVYYCSRFVGRIFQKNQKGSFIITASMSAHIANVPMNLTPYNVTKAAVKHLAKSLAIEWAGFARVNSVSPGYCDTGLNDHLSRSFRGKMWSLVPVGREAQPFEIASAYLYLASDASSYVTGTDILVDGGYTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWT6</accession>
    <name>A0A1E3NWT6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BioF2-like acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_65423</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036369.1">
        <property type="nucleotide sequence ID" value="XM_019183908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWT6"/>
    <dbReference type="STRING" id="683960.A0A1E3NWT6"/>
    <dbReference type="GeneID" id="30201154"/>
    <dbReference type="OrthoDB" id="10061051at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038740">
        <property type="entry name" value="BioF2-like_GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13480">
        <property type="entry name" value="Acetyltransf_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="BioF2-like acetyltransferase" evidence="2">
        <location>
            <begin position="626"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="799" mass="89456" checksum="729351AB6D3586C2" modified="2017-01-18" version="1">MSDLSEKVGALASRKAGIIITKYHLHPKFLVSKYAMVLYAIAMNLTHVILFAVFSKDDGGDVSIYSLLGLVLDTIAFLMIAFNRESIKAKLNKQKYALLRKVLRIFQAIWVTFHGIMVVLKVVYSAASLTAPYYTGSLANNNEDYVTALAVFAVASLFCDCIVFILLVNVLYIKASFQASRFVLLVVSIFQFLLNIGVVQVTITSLKESDLSSLKSQKFFQQETALFFHTSAFVVCLSILGVYVSIMDVDEHHKLLKVTKKFFTFFKVYGVIAFLFAMASFVCSLATISKPYYKHVVNRKVAPVLFGIIMGTCVTILVSASIYMRPPSKKNKLKVEEIDLSMLTKAQKAEFAKLITYNHNSNSGISGEAALSLMESYALSVLDGMSCKVLRVYKENVDSKLPSYENYKAWDLLDRQNMIFDDDFEVLGSVETLVGDAKPLSKNQLKKLQKKKDKTKGTALFTSENICTEDDYAKAKIFYEKLISTEALVLLTLIENYDMTEAVPGYFGRVLSRFFGKDSLLKLLTIRFGLLGFHWPFRRATFYCSTTKKPVARSAAVMEAITDWNSSLKSGEKCSMILDPTYDNDQSSKAIEASGWNQVALPASHVIDLRPYKNKSLKFYLKQIKYRNTDTKFSENGGETIETFEFTEQHSHQVIDLWKNIAAKRTTGGNTSVLIDPNEKFIMSMGQANSNSDRSILFLKLGNDILASSVLYRLGDTITSDLQGIDYSIGKDYKIYFVMMQKVIEIALKEGKSFVDFGPTTEKPKMDIGCQSIPLKGAITAPNFFLSTSVKFAASKVHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWW6</accession>
    <name>A0A1E3NWW6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein CSF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_81007</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036886.1">
        <property type="nucleotide sequence ID" value="XM_019185161.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3NWW6"/>
    <dbReference type="GeneID" id="30202407"/>
    <dbReference type="OrthoDB" id="10051416at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140268">
        <property type="term" value="C:endoplasmic reticulum-plasma membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904121">
        <property type="term" value="F:phosphatidylethanolamine transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006113">
        <property type="term" value="P:fermentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055091">
        <property type="term" value="P:phospholipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029636">
        <property type="entry name" value="Csf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056779">
        <property type="entry name" value="Csf1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048636">
        <property type="entry name" value="Csf1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32085">
        <property type="entry name" value="PROTEIN CSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32085:SF3">
        <property type="entry name" value="PROTEIN CSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25038">
        <property type="entry name" value="Csf1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21678">
        <property type="entry name" value="Csf1_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Csf1 N-terminal" evidence="3">
        <location>
            <begin position="34"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="domain" description="Csf1 N-terminal" evidence="3">
        <location>
            <begin position="1252"/>
            <end position="1504"/>
        </location>
    </feature>
    <feature type="domain" description="Csf1 C-terminal region" evidence="4">
        <location>
            <begin position="1667"/>
            <end position="2919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2919" mass="330493" checksum="75BD72BCDA8F0623" modified="2017-01-18" version="1">MDASSQFASVSITRTKDFSWVFFADWFLTYFLAIGIIFYFSRFIGVFVSYALRVLLWKTKKIKIDVQSIKLSFLGGRLFFRNFTMIDRDQTICALQGSITWRYWIPRVRKTELELQNGDPDGNIELNSKLPCRIIIEVDGLEVFVYNRTVSYENIMSQLSAQKNTRENKEDDKANYDVFSNSTSESTIRPDERETASPSSFLMKILPIKLKVNKGAIVAGNKTTQAILVVSYATGDGIIDLLKADNPLDEFRQSFQIDFERLQVALKPNISYQGEKSESSGLFFQKPSKKKYANRWMLYRSLINLWNIVIHKHKEKGQQAEWRGLAQYIDADTESINNVKEREHEYGKYTNILDSDFCSVEYYYDLPGPVPVDAPPTNPLDGLDIGNGGSSPKFGMNVKLSNSTIFYGPWAERQRHSLHQMLFPTLCRDGVPQGKLKPGKLRIYTQYQLFVEIMDEAIIRVPIREFSKNEEFAKIAKEAPDLKRPFGWIELKMDKGSIINVKTGLIASNSGYDNRMTMTLQNLEVRSSVNHDIMMRCKEHVITADIGYPLRWNGHTIWTFLNESKNVDTFFLREHITLLSDMFTDFASGPATPYELFRPFTYVLDWKVSKYAIYMNVNDANIINNPLDFNDNIYLSFQGDTLDINVHIPLEAVLRKVNTISYTLSTPQLSMMLDTPPWHTLNNFLEHKEVGRSKDFKIEGSYTYHSEVGLDLVDTIIIGCKGKYMVLKCYGFVVKYIISIKENYFGDFTTFKTLEEYTQLFNEGNENDNSQQESTIDQEAERKRAFKRIENEKDVHFSFDVEEGRLVLPSNIYDCSSNMSLFFSTLNMDLRFTNYYMDFEADITPAKGIYVDKCGPQSVLDFRGYESLERHDLFIDGLAIHGHRMFGLPPDELKYFCKWDLAPGEVDFTGRAEALEGLISSVIKLGFGYKNLENNLLIDEPEIHDVTSVSLVLPKVHIKLDDPQIDSTVVLELSKISFFFIDVSNPRYTERASAVVENILLNNDFAIERAKQDDHVLQHDGPFHRVPFFLREELRNAEYNGLYGAIVPSFSIPDGSPPITSDTIDMVFDDLGLDSDPITDDSSSEASSMKSQDTSMDFFQDFKSDFLKSPGGKPQKNNRKKNYFKTVAGSYINPTGNYRDQDFTPSYTADPNYEYDNFILNFGDIDVTAAPNSVSDLLKFADQYLTRSMIAVLDDLHIAVLKKLHLLRNMKPAVKNIRLVTPKVSLNFGDFAWDPKSEPSFNLDHLGVVLENPSLACSFTKTTENTEEAVIALHILNTVVGLYEAKDMSPFKLTMEDLEIWLNKTTKKTESASLRKFDVELQSDKVEWLKAFIENLINSGSSLLTEALRIKDNVKKSEIELLYQISLASTIFKVEHDPAVITRPAYIIRLSRQHIRANDSWKIITRLRHIYENLPEQWINEHQQIFLQKNFQAPDTAENDVVDVFSKWRSWEFDDMERTYIFRYVFSKILHEALMNSIFECSVEDAMVKLSAHDCDHDFIALDFLEVSGTKSFVEALNSSLSGYDNSNSTSVRVALIRGKLSPLTQVLKSFKDMKMNFTKPSDDSPASVSDSETKDNFGKKVAQYALIVDSYDFKIDLYTISAGISGSEVALTALHSLNDKKQDITTTFDSKRFTLEVRTDKAPSITYTISNFGFNVSSANDFKAGPKVLDVHAASASFVMQQGTDHYTTLLCNVNDDLHKLKLAFVYEGSSKREVLQSSSKSTLWQEINSATARLKVKKLSWNMSVVDPLSIVGSMENYEVMVSLANFKVVNALDLSSFTMSIKSKELEILKLSSKSLSLVSRAVFESKKVMEILVHSGSTVLLIPDILPLAKHVNQTISQAKSSVSRLKNVLQMLKDSNKSNDLNTSTVPTNVNIEPIDMLKKISFVSDTFLVAVKVANSTYGIDLKGISCSFQDFDSFDVGPMKVKPFGSFEVESTKLVIKDRQIASHISTIFDVNFGLKVFNSYQDDAVLQTLQIESAYCRLMLHPRTIVKMIEFGHDVSYLFGLVDLSPSKPKQPSTDKIKFISYFHSIHMLFYNFCVGFMFEEPSDDYAGIIAGCEKVFVISEESYGKLSLIHAYFSIANGQSSSDFYSSVNELESPNRAFLPNMQVMYAVKKANNKKDFMIRVTGEELDVKFVSSSIVLIEQTLKSVGTIESLKKKIRKYPKKPPSTVPQEGYKLPSDVTSITCFMNFAGGLVKLYRSDDFELLEDPYSPSFELRTPAVKIAAEYSKTSGLKDHHVNFEVFTSSSNNTLYSTCVPVLMDIWHSVKSLGGSSKSSSSVQVKKDEASSIPKDTEFLKVLTNFHVNLAIHIDKQELSLSCEPSAKVQAVVGIESIDIRLGTNDEDVTQPLSGILSIEKLGASLQHIYSREISGSVEVDQLIITFLLTGPEKLKVYAAGKIENVASYVNLKQLQDLDLFKDLWFPNENHPHSSSSSLRSVVSIANSAHDENLVLRFQNVSSSRAVPWNFDFMILNVNLEVDLGQSLGILTLELDRFWAASRKKTNWEQNMAIGFDTISLKSKGRLSGHLSVKNIRVHSVIQWSLVGDSFDIPLVLLSFGLQSIEAKASFDYNVFLIARILNSYITVYNQIDKEKVLQDKLVASTICQSIEIFTTALAASNILDIYNTISRIRQDNKRSYREDLKTSKADSGDHKKSLDILNVIATLRTELEVKVGKFLLQVYPGSLLDSQVLIIDVGGLTAHFEQKLVEDMIGTDLKLKLHNTSVALSSFRKQLPEELLTEMSVEKYIIHAATAKGGSIFIFPSLESAMQTWQAPKSNIIKYKFKSQFGGKVEVRWNLGSVNFIREMWATHARAFSSRLQKPVDVDNPNVPIYEDVNIEEKLKDVELEGKYVYIAIEAPIIDAPQLRDLGDATPPLEWFGLHRQRFPGITHQFVIVGLQKLVHEVEQQYGRVLGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX66</accession>
    <name>A0A1E3NX66_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PXA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_65437</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036384.1">
        <property type="nucleotide sequence ID" value="XM_019183911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX66"/>
    <dbReference type="STRING" id="683960.A0A1E3NX66"/>
    <dbReference type="GeneID" id="30201157"/>
    <dbReference type="OrthoDB" id="5582218at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003114">
        <property type="entry name" value="Phox_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF3">
        <property type="entry name" value="SORTING NEXIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02194">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00313">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51207">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PXA" evidence="2">
        <location>
            <begin position="78"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="465" mass="53660" checksum="115AA35EC716D0B0" modified="2017-01-18" version="1">MSKVAPNDRKIPSRQRMVKLTNQRPNAKATFQSQSSSTETIVSEKDTPPYLQQLIKKIFYPNSQCNEYKDALPALTSSSEVDLQLYSLIGLLFKLFVHSWYQRLTDDHEFLDEIVDITAHITRNLETRLKRVDLTQLLLDDVPLILNDHLEAYRKIHEELGSRFLPDETMDKAMDHIYNHFALMDDDETVEADYLKVLSKNVLKYLLPHDDLNSTLVEEFMKSVLSDLLLKNIIEKIIQPYQIFEIISIACEFLVDQDGGNEVKEDDRNGFNNIQTFFSQVIDLVSKTIFVSDKKRPTSVNVYSLSIFSFLNNLFQINNTRPVLYAFIKSTTPIASQAPVNDLLNNLIQNTLVKSLRNESLIALIIQKLRNNLFPSDDEMGPPRIVPDLEEFEKIRKTTKDNLKSVCNKFKLLSNLVIASESELDDTLDDFLTTFENKRMNKNLIYRILDLIVIKLIPELCGMQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX90</accession>
    <name>A0A1E3NX90_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Flo11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_81084</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036965.1">
        <property type="nucleotide sequence ID" value="XM_019185173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX90"/>
    <dbReference type="STRING" id="683960.A0A1E3NX90"/>
    <dbReference type="GeneID" id="30202419"/>
    <dbReference type="OrthoDB" id="10684707at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025928">
        <property type="entry name" value="Flocculin_t3_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13928">
        <property type="entry name" value="Flocculin_t3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="130"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="358"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="158"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="453" mass="46453" checksum="FCE0B52A8B715730" modified="2017-01-18" version="1">MTGFIKDGEPHWNVVVPGNLGTWSSVNLKGSTDGSHYLLRGVEILLEGDSASGISEVSDYEESAAYSDSIEYDSELIFSIFAHAVAGKDYVHSVEVWVDLVDEFGLQKRDAHKYELSATIVIDASISSSTSASLSGTSTSSDTAESKSTGGISSTSKVQTTDTSSSIPTSEGDGASRTQVSNVGSVVSSVASDFPSTTIITITSCSNGVCSKVPVTTGVAVGTLTTDQIVKTFTTLCPLSPTTTEKISGGVENLRPGSSVTLKSTITALSTTVVTLTACDSGVCSKMPTTTGVSVATKTINGVESLLTTFCPLTTTVTITENHDSSPSASTVDNSFHIDADDFDNVFVSGTGLASDEFPQETTINENPNSTGSNGAPNSATVTSEIGGVSNAFNEGFTTTALQKASSTVSQETSATFGSDGTQSAASISTYEGDASKSIISLGSLLAACIILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXW4</accession>
    <name>A0A1E3NXW4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Urea active transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_64881</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036739.1">
        <property type="nucleotide sequence ID" value="XM_019183824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXW4"/>
    <dbReference type="STRING" id="683960.A0A1E3NXW4"/>
    <dbReference type="GeneID" id="30201070"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11476">
        <property type="entry name" value="SLC5sbd_DUR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031155">
        <property type="entry name" value="DUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154:SF3">
        <property type="entry name" value="DUR32P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="296"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="405"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="462"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="501"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="590"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="626"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="542"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="669" mass="72716" checksum="EA770106085578A9" modified="2017-01-18" version="1">MATESLDLLSKSTGYGVLIGVGAAFAIFIVISTKLSNKYLSENSQSTEMFMVANRSVGVGLTGNAVYSSWTWATALLWCVTMVYLYGVQSAFWYCAGSSIQICIMSVLGIEAKKKIPTSHTCLEIVQLRYGRTGHLLYMVLCLINNLVSASSMILGASGAISAICDNLHIVACTLLIPFGVLLYTAVGGLKSTFLTDYVHSFILLIVMCYICTAALTNENIGGLDGLYDKLVAKEAAGELRYIEGNYKGSFITGKSQGAVFFGLIHSIGDTGLTVMDSSFWQKSFSADVRATVPGYLIAAVLIYANYWALGMVLGIGNVILEDSPIFPTYPNKMTTYEVNSGFGLIYTLKALLGKQALGGFFLVLYLGVTSTVSAQLISVSSIISFDVYRNYINPKATNKQLINVSHLGVIFFGLLSAGFSIMLHYVGVDMTWFSYFYSMLNTPGVIPMIFLITWKHQSKAAFIISPILGIIAGLAVWTGTAYALYGSVTIKTTGEQLPCLYGGLTALLLPGVASVIISLIKPANYDWNKLTSQSNLISVNDDEDDGSTTKDNNTSEGSLKEKQETTLASNEIESVDIKDGKVMSRYTKLAYGSTIFVTLVTWVVWPLPLYRDYVWSKAFFKGNVVMGFVWLYAALVVIGLFPLYDGRRSLKKVFYGVLGKSDTASQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXZ9</accession>
    <name>A0A1E3NXZ9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Origin recognition complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_19732</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the origin recognition complex (ORC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the ORC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037087.1">
        <property type="nucleotide sequence ID" value="XM_019180839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXZ9"/>
    <dbReference type="STRING" id="683960.A0A1E3NXZ9"/>
    <dbReference type="GeneID" id="30198085"/>
    <dbReference type="OrthoDB" id="346673at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007220">
        <property type="entry name" value="ORC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056772">
        <property type="entry name" value="RecA-like_ORC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056773">
        <property type="entry name" value="WHD_ORC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14052">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14052:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04084">
        <property type="entry name" value="RecA-like_ORC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24882">
        <property type="entry name" value="WHD_ORC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Origin recognition complex subunit 2 RecA-like" evidence="6">
        <location>
            <begin position="52"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 2 winged-helix" evidence="7">
        <location>
            <begin position="290"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU368084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="360" mass="41418" checksum="09C4080EE2F5EA4E" modified="2017-01-18" version="1" fragment="single">DKALFLDGPEGYFDQHRTRVKTSNNSMVQAPPLEYDELNSFVLLSGFLHNKEKQKLINTYKEMYTQWYFELSEGFSLVFFGIGSKRKLLLDYAEEYLTKMVDAPTIVVNGYNPATVFKEVLMTIVSTLNLKKVPKRLGDLVDHIVEYFQKKDHSIAKLILLIHNIDGPGLRDEKTQVYLSKLASIKQFYVLVSVDHINAPLLWDSVSLNNFNFLWHNTTTFADYLTETSFQDLLTLGQTQKSAGSKGAKYVLSSLTTNSRSLYRVLVSNQLQNMEDDLSSKKVQNDNQLKGTIKHALEYKKFYTLCAEEFISSNEINFRTMLMEFVEHKMAVLTKDQSGTEMVFIPFSLEELQKLLEDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYA8</accession>
    <name>A0A1E3NYA8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Signal recognition particle subunit SRP72</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_80204</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the SRP72 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037251.1">
        <property type="nucleotide sequence ID" value="XM_019185018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYA8"/>
    <dbReference type="STRING" id="683960.A0A1E3NYA8"/>
    <dbReference type="GeneID" id="30202264"/>
    <dbReference type="OrthoDB" id="5421607at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013699">
        <property type="entry name" value="Signal_recog_part_SRP72_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026270">
        <property type="entry name" value="SRP72"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14094">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14094:SF9">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08492">
        <property type="entry name" value="SRP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038922">
        <property type="entry name" value="SRP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 9" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="Signal recognition particle SRP72 subunit RNA-binding" evidence="11">
        <location>
            <begin position="521"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="530"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="530"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="540"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="562"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="581"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="600"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ58044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="610" mass="68925" checksum="E998E2803CF24660" modified="2017-01-18" version="1">MSSLNELFKKLEVHSANEEHQEVFDTSYEILKSSPHDARAFRWTIVSSINLDQYLTALKVFKNYPQADTGDLLLENLYVYYKLNLNKELAQHFEDLGDKVNENKGLLHLKAQFLYRTGDYEAALSHYHELINATIDEDEDPEVLDLSVNERAILADGYQVGVFNRFNSKLPLTPDREDTYDLIFNEALIHLGLKNYSKSLELLDIAEDKCKEANPDEEDQLNETFPIRLQRAYVHQIIGEFEKAKEILTGFDLSKLSDSLNTLVLNNNLISLKNIESPSLVLKELDYPNVLTKLSPRFSILQNNKFFKNYAKLSTQVGKNISTNKSLNDFTKSALASLSKAGVLLDQDDLKTQAKKSFKYAQSSKKLSASLAAAQLNVNIGNLDAAATVLEILEPKDKLLPGVSSALLSLYDQLGSSKKRLALFNEIYELYSNKSEFPNIDEYDFIKILALKFHNVETEKSKTLLNKLNNYQSDDLISIVLSQDSSDSSKLQPVEELTAGLNVDELISQGVERLSKQQVISSSANKVVKKRVRKNKKLPKSFDENKLPDPERWLPLKDRSTYRPKKGKKKLTKDTQGGVADNTTEENFNATPKAKQAKPAQKKSKKKGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYL4</accession>
    <name>A0A1E3NYL4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_70209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037473.1">
        <property type="nucleotide sequence ID" value="XM_019184195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYL4"/>
    <dbReference type="GeneID" id="30201441"/>
    <dbReference type="OrthoDB" id="3980926at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="232" mass="27411" checksum="035AB8A8E1796A2B" modified="2017-01-18" version="1">MTSTAKRSYSPTIDDIRQFNIKRQRILNDFEGLSLSSASASPASKPKPSSSKHKEYIPDIEEFLINNQDDQDILESSKHIDIDNQDSAHWIIPNSIISIPGVDSKQVEFSYDRLKSRKLFENRYNIKNHDQYITNDEILYDYEILRYWSLMKYIKSPSLYIYEHWKHWYFNDCVTHLKHDDRIEMLPDDYSGEIDLTNEIQDYDNDLMIDDDDQNSTSYNANPSLTDEMDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYN4</accession>
    <name>A0A1E3NYN4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NFACT RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_34320</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC25 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037440.1">
        <property type="nucleotide sequence ID" value="XM_019182172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYN4"/>
    <dbReference type="STRING" id="683960.A0A1E3NYN4"/>
    <dbReference type="GeneID" id="30199418"/>
    <dbReference type="OrthoDB" id="200398at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039730">
        <property type="entry name" value="Jlp2/Ccd25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13049:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13049">
        <property type="entry name" value="DUF814-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="4">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="194"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="137"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ58233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="220" mass="26231" checksum="8C5F1CF967000EAB" modified="2017-01-18" version="1">MVYYFKSVGSDGSFESNPRFIYMGKDKYENEDLIKYGFEEDVWFHVDNLSSAHVYLRLEIGEKWDEIPEGLLIDCAQLTKANSIDGNKRDNITIIYTPWANLKKTKGMADGEVSFKNSKKVKKIHVQFRENSIINRLKKTREEIKGKEHLIEAKNNRDKEHVSNLLQLKYQEKLQQEQEKKRIAEEKHRQETLYDDLWDDNEVNKTSNQKRMYDPEEDFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ59</accession>
    <name>A0A1E3NZ59_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_64605</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037676.1">
        <property type="nucleotide sequence ID" value="XM_019183771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ59"/>
    <dbReference type="GeneID" id="30201017"/>
    <dbReference type="OrthoDB" id="4095537at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133583" evidence="2">
        <location>
            <begin position="19"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="153" mass="16583" checksum="4C2B554E6332D6E3" modified="2017-01-18" version="1">MQLFKSLLYVLFLSFVTAQSTSSWMTYTKTITNHGRTYTKTVNELYTGQETTLPTSGTVTTTITVSNSKETYTKTMVQTYGQALGTTTHLTVKNPSETYTKTLSTEIAKNEASAASESSGSSSSTSSSEAGAGPLKEFNNMFAYAFIIGAMFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ88</accession>
    <name>A0A1E3NZ88_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">U3 small nucleolar RNA-associated protein 11</fullName>
            <shortName evidence="6">U3 snoRNA-associated protein 11</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_85144</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the ribosomal small subunit (SSU) processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Nucleus</location>
            <location evidence="2 6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the UTP11 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037199.1">
        <property type="nucleotide sequence ID" value="XM_019185848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ88"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ88"/>
    <dbReference type="GeneID" id="30203094"/>
    <dbReference type="OrthoDB" id="29058at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007144">
        <property type="entry name" value="SSU_processome_Utp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03998">
        <property type="entry name" value="Utp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015952">
        <property type="entry name" value="U3snoRNP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="247" mass="29326" checksum="FE0BE364AC00DA9D" modified="2017-01-18" version="1">MAKLVHNVQKKQHRERAQPGDRKKYGLLEKKKDYQLRARDYHQKQATLKSLKQKVQARNPDEYYHAMTNKKTDSRGILISERDTEALSTGQVKLLKTQDSSYIKTLRNEEAKKIERLEKTLNFKSSGKHTVFVQDEIAKKEFDPVKFFQTDRNLLNARENRLRLDQLQTDQSLVPRLDEDAMPKESLDKKRIKKFSQLKAHLERQKELKEVEERMDIQREGMKNGNKKKVVDAIGRTTYKFKKERKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZE2</accession>
    <name>A0A1E3NZE2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-dehydropantoate 2-reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_85536</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037740.1">
        <property type="nucleotide sequence ID" value="XM_019185905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZE2"/>
    <dbReference type="STRING" id="683960.A0A1E3NZE2"/>
    <dbReference type="GeneID" id="30203151"/>
    <dbReference type="OrthoDB" id="3978163at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000017">
        <property type="entry name" value="2-dehydropantoate 2-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051402">
        <property type="entry name" value="KPR-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708:SF30">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708">
        <property type="entry name" value="PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase N-terminal" evidence="1">
        <location>
            <begin position="6"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="2">
        <location>
            <begin position="197"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="37710" checksum="554FD08FEE46A3CE" modified="2017-01-18" version="1">MTKSNVLLIGTGGVGTIVAYGIDYVGKANLSVVVRRDYSKVIESGWDLNSCDYGEIKGWKPQNIYPNVEAAKQDGSSNIYDYVIVTTKNLPDITKIEELIEPVVTPKKTTIVLIQNGFDIGRPFIKKYPQNVVISGVSHIGSHNHHGVITQTQNDKTLISYFENPNLSKEHQEAVTKDFISIYKNDKNTCTYFPDAKWYRYRKLVYNASLNTVAALTGVDTGRLELSGGLEAISIPAMKEVISIAKADGVELPGDVINLVVHSDDGDWFEPSMRVDVKKGNPIELEVILGNLLVVADELKVSAPTLKILYELLKVVQFKLKEGQGLISLPAKRPINDKVYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZR8</accession>
    <name>A0A1E3NZR8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphatidylglycerophosphatase GEP4, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_32399</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037896.1">
        <property type="nucleotide sequence ID" value="XM_019181954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZR8"/>
    <dbReference type="STRING" id="683960.A0A1E3NZR8"/>
    <dbReference type="GeneID" id="30199200"/>
    <dbReference type="OrthoDB" id="198652at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000165">
        <property type="entry name" value="HAD superfamily phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027706">
        <property type="entry name" value="PGP_Pase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010021">
        <property type="entry name" value="PGPP1/Gep4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01668">
        <property type="entry name" value="YqeG_hyp_ppase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF25">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROPHOSPHATASE GEP4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09419">
        <property type="entry name" value="PGP_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="23407" checksum="0D40CA36A6991D4E" modified="2017-01-18" version="1">MNVSATLNVFRLFFTPSYSVPHLTVSSFDKIPIPLKFPNHQPNIRAIVLDKDNCFAKAHDDKVWPEYKEAWDKLRTTYPGASLLIVSNSAGTNDDKNHEQAKVIEERTGVTVLRHTTKKPGCHNEIMEYFKKNNIASGPEEVAVVGDRLFTDVLMANMMGAWSIWVRDGVVRSESLICKIERSVYSQFTQRSNPLLPPIPKFKTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P016</accession>
    <name>A0A1E3P016_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_63184</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037885.1">
        <property type="nucleotide sequence ID" value="XM_019183504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P016"/>
    <dbReference type="STRING" id="683960.A0A1E3P016"/>
    <dbReference type="GeneID" id="30200750"/>
    <dbReference type="OrthoDB" id="4489171at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14270">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055335">
        <property type="entry name" value="Ucp6/RUP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39597">
        <property type="entry name" value="UBA DOMAIN-CONTAINING PROTEIN RUP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39597:SF1">
        <property type="entry name" value="UBA DOMAIN-CONTAINING PROTEIN RUP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBA" evidence="3">
        <location>
            <begin position="5"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="82"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="622"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="439"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="625"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="643"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="675" mass="76768" checksum="67B86129AB61D923" modified="2017-01-18" version="1">MGNADVSDAAVKQITEMGFPEEQAIQALRATGGDVNEALNLIFQGADFSQGQELLQQDAQLPDYVDPGYNYQVDEKRSIPIESGLINDKIQPPSYEQEENPPPVPPRQNRPIHIEDIQHRSQSEVSLSDSGYLPDSETYKYQQQLQVEPEYSIYEDLTKYQKPDDAPSLLMPVRNGVLESYLNPFLTVLHSIPAFRKAVYNQTFETLGFHPRWYKGELINVPDGTAIKAKNGETYDLRFLLEVQRIFAFLDGDSGRYFASMNNFIRSFPKIAQKKFGSIDNIYEAYAVFYSVLASQLDVSGSGNALSFFESELEDLADANKKFGMFHVEAEELKGDLYSTLHSILWGDDLDNQQFLSKLSDVMTMSFEPSYDETIISKGVLISEKFYPQIYTTDFKETISELLEERNSFDKKKRLISRELLNLRAFNGKHVSSILSTSIEFLSSELKNLNEEDKELADAVKDLESIKADNHEKIISLTDKQNTLQDEKAKINPYDVNAIFKRHGSVPEPYLLTGVILSNNEFYFLRKVKEDLIDLEQNQNQWIRVLYEPKYGNDLRCSVSSFEEVQRVVHATTQGQYETSLVLIYVKESTWTTEVEYEVPESVTNFIAKDKLELEKSLELLASSTDEELSDEEGDEEEDGNGMEQSAGVTNQEGEISLKGNDENSEDKENAPPTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P035</accession>
    <name>A0A1E3P035_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_70236</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the importin beta family. Importin beta-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037509.1">
        <property type="nucleotide sequence ID" value="XM_019184197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P035"/>
    <dbReference type="STRING" id="683960.A0A1E3P035"/>
    <dbReference type="GeneID" id="30201443"/>
    <dbReference type="OrthoDB" id="951172at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010458">
        <property type="term" value="P:exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000028">
        <property type="entry name" value="Transportin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="11">
        <location>
            <begin position="31"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="429"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="322"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="327"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="347"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ58302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="879" mass="98169" checksum="F6D7A19121743F63" modified="2017-01-18" version="1">MSWEPNPTAIEQLKHILAGTLSPNSHERSQATEALDQAKIQEDFHNYLLFILVNDKSTTSQIRASAGVNLKNDMIKNFNVKTNDYLLENILKGLLDDDPFVRNITGTVITSIFSTLGVAKWPFVLPQLMELSETGNVTSQEGATGALAKICEDSSHILDTDYNGQRPLDFMVPKFIALTGSNSPKVRANALHCLNQIILTKTQSFLVHIDDFLQRLFSLAGDQDPAVRRNVCTAFAYVLEARPDKIAPHLDGCVNYCLHSITTSEDEVALEACEFLLALATSDIPEDLIKPYLPNILPVLLKSMVYSEMDIFLMDNTDDADVEDRDEDIKPTNAKVKDAHNQKNGGGDDEDEEDEDGDYSTEWNLRKCSAATLDVLTSVAPSEVLQISLPILRENIVSEQWPIREAAILAFGAVSEGGIELASQQLPALIPFLVERLQDDESSVRQITCWTLGRYSAWICSEAHKGGLYSNYFIPTFQSIIACSLDKKKMVQESACSSIAQFIENSETDLIQPLAADLVENFKKCFQYYQRKNLVILYDAIQTFVERIELDSGAIEAILPPLLKKWELLSDDDKELWPLLECMSSVAASLGEKFAPYAVQVYERSLRILQHCIEMDKQCKSDPNIQVPEKDFIITSLDLIDGLCQGLGEHSGELINEHLIKLVIECFNDPIDDVRQSAYALLGDIAIYIPNTLGSHIDEVIISIGREIVARNQENFAVCNNATWALGEISLRINLNNYLEKLVGILIDLLKSQAMSTVLENAAITIGRIGISSPEFFSPHLNEFLLDWSAHMMYLEENEEKETAFQGMCNIILTNPTGFNNESLVAFVNTITMYLNPGQKLAEIFHKLLSGYKEMLGDSWTQFLQTIDNGAELTGRYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P047</accession>
    <name>A0A1E3P047_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MaoC-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_33196</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038033.1">
        <property type="nucleotide sequence ID" value="XM_019182042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P047"/>
    <dbReference type="STRING" id="683960.A0A1E3P047"/>
    <dbReference type="GeneID" id="30199288"/>
    <dbReference type="OrthoDB" id="3257538at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019171">
        <property type="term" value="F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052741">
        <property type="entry name" value="Mitochondrial_HTD2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28152">
        <property type="entry name" value="HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28152:SF1">
        <property type="entry name" value="HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="31113" checksum="30C1FACE387A3406" modified="2017-01-18" version="1">MKGRQWILMDTMSSTHSQYLKAALYSYFNVQKPLGKFLSNGYHLLYFNPKHEETELSKDGYDSHQIPANLMFKRRLWTGGQIDFLHPVAYDSQAVCVESIQNSKSFGENHMVTIQREVVADGLISLVERRSLFYTNDLYKKSTPQFPQKPAIYTHELKPTDILLFRYSSLTFNAHRIHYNKDYAMREGYPNILVHGPLTVTLLLEWFSTLFGDLVVKSFKYKNIAPLFANEKIKLCLGQTSDLKYDTWIENEDGELCVSGTIEVINNDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P075</accession>
    <name>A0A1E3P075_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_84555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038046.1">
        <property type="nucleotide sequence ID" value="XM_019185729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P075"/>
    <dbReference type="GeneID" id="30202975"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="216" mass="25086" checksum="8C9DA034D6D45AC3" modified="2017-01-18" version="1">MLRQIQAQSTTNNSSQQSQAQTTSHFQEIGTNQNHHDRHQQGKITTQASTDTMDVTNMIDYNTIHLFTMSSNLIDPKGCIKFGNYTIAGVIKFLVELNTFCFGESTKSFDTLSKPQTQTLLLKFKQNVIFTELHSSHDDLQSSDNFIFNKAGVYFPFRYWFQRRKVLNNHSLEILVPMRFMLGYYYLVFIIPFTYYWESFPVVTLGLLFFICSLCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P089</accession>
    <name>A0A1E3P089_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL4m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_79429</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038088.1">
        <property type="nucleotide sequence ID" value="XM_019184890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P089"/>
    <dbReference type="STRING" id="683960.A0A1E3P089"/>
    <dbReference type="GeneID" id="30202136"/>
    <dbReference type="OrthoDB" id="275876at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013005">
        <property type="entry name" value="Ribosomal_uL4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746:SF6">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL4M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="252" mass="28328" checksum="CAB12616F1D376F8" modified="2017-01-18" version="1">MFVKQNSILMNPIKQQIAKLATVATNTIKEHAQEPPKYVLSSLRSFPSLKPTTFQPIPSSLLNTPIRRDLLWKAVIFDADNARVGSSNPKARNQMGYSNKKLAPQKGTGNARVGDRGSPTRHDGGVALARNAPNFHQTELPTKVYNKAVRIALSDAYRNGKIFLIKDDELDLPTNDDIAIKMFLRKFEISNKKLLFITNGLKENLLKATEFNSKKIDVAEKEGVKVKDLLKADKIFIELKSLQYFQDIYGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0J4</accession>
    <name>A0A1E3P0J4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribonuclease P protein subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_32306</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038112.1">
        <property type="nucleotide sequence ID" value="XM_019181941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0J4"/>
    <dbReference type="STRING" id="683960.A0A1E3P0J4"/>
    <dbReference type="GeneID" id="30199187"/>
    <dbReference type="OrthoDB" id="124041at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000171">
        <property type="term" value="F:ribonuclease MRP activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034965">
        <property type="term" value="P:intronic box C/D snoRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000294">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, RNase MRP-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="entry name" value="Ribonuclease P/MRP, subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016848">
        <property type="entry name" value="RNase_P/MRP_Rpp29-subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027081">
        <property type="entry name" value="RNase_P/MRP_p29_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="Rof/RNase P subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR027081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="265" mass="31061" checksum="52CEEA51BC926F65" modified="2017-01-18" version="1">MDRKYPLESLILSRSYENSSKINELLEQRYALNDKPFLVLRPTEGEREREQTLLTRVKNSKIQHQKNQKKDQTKIVFKRYIKKSIYLQKKLKSRLNKSNIDDVCKLKEFKKIPHFEDFLKLNELWIGYIQDLLQLDLQNKRNISVPQVLTKLSSADYNGCFLRVLKSKNSVNIGKNGIVIYDSKNFFIIVEPRDANDPKSIGGLKMIEKKGSVFSFVVPLFDIQNEEIDANDDNYLEFSIIGSRFQFRAPDRSGKKFKSRNVDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0K4</accession>
    <name>A0A1E3P0K4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Choline kinase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_31677</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the choline/ethanolamine kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038213.1">
        <property type="nucleotide sequence ID" value="XM_019181867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0K4"/>
    <dbReference type="STRING" id="683960.A0A1E3P0K4"/>
    <dbReference type="GeneID" id="30199113"/>
    <dbReference type="OrthoDB" id="10267235at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004103">
        <property type="term" value="F:choline kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004305">
        <property type="term" value="F:ethanolamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05157">
        <property type="entry name" value="ETNK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007521">
        <property type="entry name" value="Choline_kin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603">
        <property type="entry name" value="CHOLINE/ETHANOALAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603:SF93">
        <property type="entry name" value="RE24176P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04428">
        <property type="entry name" value="Choline_kin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01633">
        <property type="entry name" value="Choline_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Choline kinase N-terminal" evidence="3">
        <location>
            <begin position="91"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="588" mass="67444" checksum="B76B8098DE1E1486" modified="2017-01-18" version="1">MSAETYSHHRRSSLRSAPTTSGSRSRSRSKPRRPSLGSRRNSAKSFISIKNELEPFTPSPTSSSQQNDQGGLGQLPSSNSSSNIQFTETDIPFINATLDHTLPIGYFKQDLLSVIQNLRISKWHRLPSHVYSDIKIHKMSGALTNAIYKVDPPSVVVLKNLNLHTYHFPTLLLRIYGPNVESLIDRDYELKILVRLSKQNIGPRLFGCFTNGRVEQFLDNATTLTRKDIHDKKTSARIARRMKELHQGIKLSKFEKAEGPSVWKIIDKWLKHVDELIESKTIVIDDKELFKTDFATFKKYTKAYRDWLYKRYESPEALNNALVFCHNDTQYGNLLFTSPVVPPPDKTNNNTTANNSTTSLTTNFSNLSLENLNEIKPSIQEKKQDRNLVVIDFEYSGPNVPSYDIANHFCEWMHNYNHETDTWKIFEEDYPKLDEQLNLIYSYILFYNDIQDVTKLEKQAKELFNDCIAWRGAVNIFWGLWAIIQNGDNNATKDHEIVEMGPNGEMYKIVFDPEDEESEVTVVDSDEDESSGNAGASSADVDNFEYLKYSKDKLLCFWGDLIQFGLVDKDALNKPDEVKLLPAKFYDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0S6</accession>
    <name>A0A1E3P0S6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_63379</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038077.1">
        <property type="nucleotide sequence ID" value="XM_019183536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0S6"/>
    <dbReference type="GeneID" id="30200782"/>
    <dbReference type="OrthoDB" id="4024240at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="87"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="653" mass="75187" checksum="ABD066203818A569" modified="2017-01-18" version="1">MAPLYKVNIPIGHAGVPFYSNSQTPDNQRSLIDKDSISSILPFVSSSINSDTFGDKDYNYHPSIGTYNQYSSVRNNSIESLEPLSFFDRLPFEIKLKIANELNQYDALNLMKVSKSFFQPAVVRLYHTIVIDPCYSQFNQEIYQAELQEHDQSVNSSTFIKMKYSLKNFLKIISQEMPIEMCKIGYYNSHYGSLINKIQFVELPDGFNSPFDLNFFTFMSVKRLERLSRFYWSSDEELSFEIVNRLPNKSNLNSLHVSVDFKKVRAGSTEFEAFRGFNNLEKLSLEPFLNSFNLFQIFKNLLEGNNDKLNLKVLRLARVNSNHNFIKNPSSSLLVLTSYIIENEPIKLSEYDMNCILNLFILFSAAETRLEDLNVLSLDSIVVSTMDARRLDSVMNLENLKQLELKNITEVQLPEDVHSPLSMNDLYARLDAGFLKHLGKNLKNLTRLSIDYRESLRDTVPAFIYNLENLQELDITIRWNVTKLCSIQSWKHLCFMYIKAILKHSKTLKKLSLDTKEDSVFCDLHKLIFTECLLELGGLTELESLRLHGYSLQPCGPLLASMLKKLKYFELFGSGAGGAPHMGLQVVHDGVLDDWFRVQHVAVAISEQNEHIEFIKIDKCLFEVQKTGKLLPRDGLEGWFESKVRVILNRVDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P179</accession>
    <name>A0A1E3P179_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminoglycoside phosphotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_79547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038217.1">
        <property type="nucleotide sequence ID" value="XM_019184911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P179"/>
    <dbReference type="STRING" id="683960.A0A1E3P179"/>
    <dbReference type="GeneID" id="30202157"/>
    <dbReference type="OrthoDB" id="191037at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05154">
        <property type="entry name" value="ACAD10_11_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052898">
        <property type="entry name" value="ACAD10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041726">
        <property type="entry name" value="ACAD10_11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47829:SF1">
        <property type="entry name" value="HAD FAMILY PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47829">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12880)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="1">
        <location>
            <begin position="55"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="394" mass="44624" checksum="542408A445DE86E2" modified="2017-01-18" version="1">MGIAPVVDSEQDVGPIRSEFNVEKLEKFFASPSLPEGTTMGPQIGWSPLIPPLSVQQFKFGQSNPTYLLTDSKGTKAVLRKKPTLNKKLISKTAHAIEREFFMLASIKNINDRRPKENHVPVADVFLLCEDEQHIDAVFYVMQFVKGRIFHDPSLSPVKSTQDRSQIWDAVLDTASAIHSIPSNELFDQLPTRFFKKPTPNPKVTYFDRQVKSLNKIHDLQSRDVDQIPHFKETSKWLLKNAPKDPEVPHLIHGDFKIDNFVFHPTKPVIIAVLDWELCTNGHPLFDLANVLQPFCMPPSLNKYFAKFDIDEDPHLVDRVLDGYIEKAKPDWDPKKLWTVGVVFGLYRVAVICQGIAQRVVRGVASSAEAQQTARLFPHVGDLAFKLTNSDPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1B4</accession>
    <name>A0A1E3P1B4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_69634</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038476.1">
        <property type="nucleotide sequence ID" value="XM_019184176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1B4"/>
    <dbReference type="STRING" id="683960.A0A1E3P1B4"/>
    <dbReference type="GeneID" id="30201422"/>
    <dbReference type="OrthoDB" id="2544694at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11578">
        <property type="entry name" value="DUF3237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="171" mass="18819" checksum="ADDA65EEDDB8D006" modified="2017-01-18" version="1">MSDPSSIASLKPAFFLHVDADEPQPIYKNAQGSLTLVKLNGGYTKTLDPKYPFDTEVEFGIDNLTTKAGRDGTITNLDCQLYLKSKKNGGGIHFAYSGVVIQGEQVVDVVLKKSNDSGFTKSYVTNHPIVTLDESNKEESWIQGKNLIGKGRFTRSESGKLAVEYYVYALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1J1</accession>
    <name>A0A1E3P1J1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SUI1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_84931</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Additional factor that functions in concert with eIF-2 and the initiator tRNA in directing the ribosome to the proper start site of translation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SUI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038556.1">
        <property type="nucleotide sequence ID" value="XM_019185810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1J1"/>
    <dbReference type="STRING" id="683960.A0A1E3P1J1"/>
    <dbReference type="GeneID" id="30203056"/>
    <dbReference type="OrthoDB" id="10248435at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043024">
        <property type="term" value="F:ribosomal small subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990145">
        <property type="term" value="P:maintenance of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11566">
        <property type="entry name" value="eIF1_SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000005">
        <property type="entry name" value="Sui1 translation initiation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005874">
        <property type="entry name" value="SUI1_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01160">
        <property type="entry name" value="SUI1_MOF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10388">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004499">
        <property type="entry name" value="SUI1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="SUI1" evidence="6">
        <location>
            <begin position="27"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="109" mass="12533" checksum="D3ABC3055FD21EC7" modified="2017-01-18" version="1">MSSIQNLKSFDPFADTGDDDTQPTNYIHIRIQQRNGRKTLTTVQGLPDEYDLKRILRVLKKDFACNGNIVKDEELGEVIQLQGDQRVKVMEFLTTQLALQKKNIKIHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P275</accession>
    <name>A0A1E3P275_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycoside hydrolase family 132 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_21265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SUN family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038635.1">
        <property type="nucleotide sequence ID" value="XM_019180899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P275"/>
    <dbReference type="STRING" id="683960.A0A1E3P275"/>
    <dbReference type="GeneID" id="30198145"/>
    <dbReference type="OrthoDB" id="5554151at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053088">
        <property type="entry name" value="Beta-glucosidase/SUN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005556">
        <property type="entry name" value="SUN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31654">
        <property type="entry name" value="SECRETED BETA-GLUCOSIDASE ADG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31654:SF0">
        <property type="entry name" value="SECRETED BETA-GLUCOSIDASE ADG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03856">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="28531" checksum="1F3FEDC99FE42252" modified="2017-01-18" version="1" fragment="single">KRGNTCQFPSDKGLVAVQKDGKNGGWAMHNDQSCTAGSWCPYACPPGQLMGQWDPDVKSYSYPGSQNGGIWCNDDGEIEEKKSGSYCYGGKGTVIAKNQVGDNVAFCQTVLPGNEEMLIPTNVDGNGKKTLAVPGSDYWAGTAAHYYINPPGVSTDDGCKWGSTDHPWGNWAPYVAGANQDDNKETFVKIGWNPVYLEDSSPFKNKKPDFGIRVTCDNEDSCSGLPCSIDPSKDGVNGIGNNKGSSGAGGGDFCVVTAKNGAKANIEIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2B1</accession>
    <name>A0A1E3P2B1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Choline/carnitine acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_24646</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038742.1">
        <property type="nucleotide sequence ID" value="XM_019181043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2B1"/>
    <dbReference type="STRING" id="683960.A0A1E3P2B1"/>
    <dbReference type="GeneID" id="30198289"/>
    <dbReference type="OrthoDB" id="240216at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004092">
        <property type="term" value="F:carnitine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006066">
        <property type="term" value="P:alcohol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.20">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042572">
        <property type="entry name" value="Carn_acyl_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF48">
        <property type="entry name" value="CARNITINE O-ACETYLTRANSFERASE YAT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00439">
        <property type="entry name" value="ACYLTRANSF_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="8">
        <location>
            <begin position="11"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="608" mass="69136" checksum="BD4C90AA5C1209E8" modified="2017-01-18" version="1" fragment="single">TFQKEAQLPNLPVPSLNDTLKLLEISLAPLLNDDEFYQLQRKIQDFKTNEISSILQNHLLKFHQNEDCYLDHLNLDHILIDHKALPRNPFLILENDPLKKIKMNQTQGERAAVLTNSALKFISSLRLGYLNQDETFGGKPLTMKPYLNLFGTTRLSEFGSIRSKTNLTSDYILILSKSQFYTLKVLNEDQKLLFSIDELSKLLDELVEEVNQVENPNSTAIGSITSDTFKNWSSSRSYLQREFKENLDKIDAALFVLVLDHSEPDNTSGEELAKVLSVGSLKIDDHGVQTGSCTSRWYDKLQLVVTANSVAGIIWDSFTADGTSVLRFTSDMFTDSVLRLGDGNYSLFPKVKLTKSEDYPRPKPEKLHWDFAADAQTSLHLAETRLTDLICTHLTNTKTLNFGRNFAKKIGVKADSLIQISLQIAHYALYGRPISTVEPVSTRIFKNSRSELYPVQNDFITKTCQIFVSDSTPSIRWQSFIDCCKFHSNNLRKCAKGEGFEKHLKALQNVYLQRQIFNQVNPEFKIPDDEIPPLLFDDVIFPLFVPDLVASNCGNPAMRLFGLTPAVSNGFGIGYIIKDDTTEFCLISQFRQNERFLSTLDWVFHQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2E2</accession>
    <name>A0A1E3P2E2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acid phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_84725</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histidine acid phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038274.1">
        <property type="nucleotide sequence ID" value="XM_019185766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2E2"/>
    <dbReference type="STRING" id="683960.A0A1E3P2E2"/>
    <dbReference type="GeneID" id="30203012"/>
    <dbReference type="OrthoDB" id="258392at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050645">
        <property type="entry name" value="Histidine_acid_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567">
        <property type="entry name" value="ACID PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567:SF142">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133649" description="Acid phosphatase" evidence="3">
        <location>
            <begin position="22"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="448"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="55646" checksum="DB84C3F827619E8D" modified="2017-01-18" version="1">MLLNSVLTTIALGALASTASAEDSDYKVVGSLIFGRHNDRPAKPASYLTPIGAESQYTIGQFYRERYFGLDSSNVKSSSNEGNTTTISGLEKDGLYVNGQIYCEAESSNVILFSHYAFLQGLYPPQNVAGTSVGNLTLAEIANGSVVQNPLNGYQYVQSNIQENATDGYIWTKGDSNCPASDAAIDQVQNSDLFKTLTNESAEFYQSIADIPFIKEKFNSSKVNFKNSMNIFDQVWVNTIHNETVAQQFNESLIDAIQFWSDKYQWLISDNQVNANLTIGAKTLMARIADKLTTTKNNGKPYLNYLTGSFNTMYQLASVMNLDIADSKFRTMPNYGATYVFELLNNTQNETFVKFSFKNGTQELNTYPIFNSTSDLMSWDDFVSNVGKISIPKVSSWCSACGYDDISSDNVIDMCVPSSDTYEQALKLESEGVDLASYKKNKMSLADAGGIGAGVTIGVFAILGGLIFLGLKLRNKNEKKSAILPSNVSDLSSNTDIESHVEKGSSHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2Y5</accession>
    <name>A0A1E3P2Y5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA polyadenylation factor FIP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_104828</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FIP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038982.1">
        <property type="nucleotide sequence ID" value="XM_019180430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2Y5"/>
    <dbReference type="STRING" id="683960.A0A1E3P2Y5"/>
    <dbReference type="GeneID" id="30197676"/>
    <dbReference type="OrthoDB" id="1917198at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007854">
        <property type="entry name" value="Fip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051187">
        <property type="entry name" value="Pre-mRNA_3'-end_processing_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484">
        <property type="entry name" value="FIP1-LIKE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484:SF0">
        <property type="entry name" value="PRE-MRNA 3'-END-PROCESSING FACTOR FIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05182">
        <property type="entry name" value="Fip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-mRNA polyadenylation factor Fip1" evidence="7">
        <location>
            <begin position="161"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="223"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="35"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="223"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="285"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="320" mass="35577" checksum="A3AF5108BCD1A39E" modified="2017-01-18" version="1">MSNEIDDDDAFLYGSEDEGDAQVSKKQKLESTTQDQKESATTEKGESKEDSNEDEGDDEDGDSDSDSDSDIEFIIGSEEPKTTTNTSQETQAQQSGTSAAPSTSGVTVPETIDEITDTTQTEAPIGDSSTQATTTDATITRIPGIDINKVGEFEGKPITSINLQDLKEKPWRQPGADVSEYFNYGFDEFTWTAYCSKQDKLRSDFNPQKVMMSMMPMGMPPMMMGMPGFQMPPGGQMPQIPQGPQQQQQQQPQVPPPPPQGLPSQPQQMSGNEKTMSLNPNLPKAPRERDPRDRMERENSWGQNDGNNYRDRERRNRGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P329</accession>
    <name>A0A1E3P329_WICAA</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_83820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019185604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P329"/>
    <dbReference type="GeneID" id="30202850"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="146" mass="17535" checksum="F9FC09C9C939C18A" modified="2017-01-18" version="1">MIKESVLTLSQVLNSSHSILIKFIEFISKCQIDENITKFSPNSFSETTMIIINFEGYEQLYHELFNDLYDLEEVLKDFKELIRKESPCFKEYTQKYHEHSLKDDEIDDGLFSQDVFTEFDQDDFEKFSKKRKIRIGIEKRKLFSEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P343</accession>
    <name>A0A1E3P343_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GPI-anchored wall transfer protein</fullName>
            <ecNumber evidence="8">2.3.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_29821</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">A acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Endoplasmic reticulum membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the PIGW family.</text>
    </comment>
    <dbReference type="EC" id="2.3.-.-" evidence="8"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039042.1">
        <property type="nucleotide sequence ID" value="XM_019181655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P343"/>
    <dbReference type="STRING" id="683960.A0A1E3P343"/>
    <dbReference type="GeneID" id="30198901"/>
    <dbReference type="OrthoDB" id="15270at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032216">
        <property type="term" value="F:glucosaminyl-phosphatidylinositol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009447">
        <property type="entry name" value="PIGW/GWT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06423">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017321">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 8" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="396"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="470"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="495" mass="55104" checksum="6EF6AD02F5B22135" modified="2017-01-18" version="1">MSSLKERKQDFVAGLSGGEIPEINAVTSIALVAYFSWSLINKYTDLFKGSKKWEPTSLIIDFLLNWGGLLLSITVYSGSPLFLAAAILLPSASLSVLFGTPIKVKADQKPASKTLTDKTYLTKKPFITAYRGGMLIITTLAILAVDFQVFPRRFAKVETWGTSLMDLGVGSFVFSMGIVSTRSIILAKFNNVKVTYISRLWKSISSSFTVLGLGFLRLFFVKNLDYQEHVSEYGVHWNFFITLVLVGPLTIFMSPLFSVIPRCIAGLLVSLVYEWFIVNREGFLNYLLLAPRTDLVSSNREGIFSLFGYLSIFLAGQSTGFYVLPSVKSKYNLFKPSDKNTIISTQTSKLTFFQRLTTVSPITGLLIWFVVYLSAFNFVSANHEYSVSRRLANLPYVLWVCAYNTGFLTFYALTDKLLDSATSSYEEKVPPSLESINSNGMILFLLSNVSTGLVNMTINTLDASTGKSMVVLFAYSSLIALVGAFLYYNKIFIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3B4</accession>
    <name>A0A1E3P3B4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_84952</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038584.1">
        <property type="nucleotide sequence ID" value="XM_019185813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3B4"/>
    <dbReference type="STRING" id="683960.A0A1E3P3B4"/>
    <dbReference type="GeneID" id="30203059"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004032">
        <property type="term" value="F:aldose reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006066">
        <property type="term" value="P:alcohol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019568">
        <property type="term" value="P:arabinose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042843">
        <property type="term" value="P:D-xylose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042180">
        <property type="term" value="P:ketone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000018">
        <property type="entry name" value="Glycerol dehydrogenase Gcy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="8">
        <location>
            <begin position="22"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="7">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="312" mass="34779" checksum="BAB5FD7CD5F280EA" modified="2017-01-18" version="1">MVELQNSTATITLNTGAKIPQLGLGTWRSTEGQAYDAVLHAIKETGYVHIDSAAIYKNEEEVGKAIRDSGVPREKLFVTTKLWGTYHRNPEAALDESLKKLGLDYVDLYLIHWPVAFKTDNIKDGNYLTVPEKPDGGRDIDIDGWSFVNTWELVQKLPETGKTKAVGVSNFSINNLKELLAAPTTKVVPAANQIEVHPLLPQTELIEFAKSKGIVTEAYSPLGSLDSPLFTNKTLLEVAKSYDVSVAQLLINWALKRGYVVLPKSVNTERIDANFKVFDLKDEDFEKVSDLVKAEGEQRFVKPDWSPFPVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3E6</accession>
    <name>A0A1E3P3E6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Squalene monooxygenase</fullName>
            <ecNumber evidence="4 10">1.14.14.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_62365</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, and is considered to be a rate-limiting enzyme in steroid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>squalene + reduced [NADPH--hemoprotein reductase] + O2 = (S)-2,3-epoxysqualene + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25282"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15440"/>
            <dbReference type="ChEBI" id="CHEBI:15441"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the squalene monooxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.17" evidence="4 10"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038987.1">
        <property type="nucleotide sequence ID" value="XM_019183349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3E6"/>
    <dbReference type="STRING" id="683960.A0A1E3P3E6"/>
    <dbReference type="GeneID" id="30200595"/>
    <dbReference type="OrthoDB" id="1678617at2759"/>
    <dbReference type="UniPathway" id="UPA00767">
        <property type="reaction ID" value="UER00752"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004506">
        <property type="term" value="F:squalene monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013698">
        <property type="entry name" value="Squalene_epoxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040125">
        <property type="entry name" value="Squalene_monox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835:SF0">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08491">
        <property type="entry name" value="SE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 10" id="KW-0274">FAD</keyword>
    <keyword evidence="5 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0492">Microsome</keyword>
    <keyword evidence="8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="429"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="454"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Squalene epoxidase" evidence="11">
        <location>
            <begin position="189"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ59780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="483" mass="53507" checksum="59D6E917277AE702" modified="2017-01-18" version="1">MTTSTEYEAIVVGAGAIGPAIAVGLARQGRKVLLVERDWREPDRIVGELMQPVGLRALRSLGMVQAINNIDATPVEGYTVFYEGQKVQIPYPFKADVAPYKPLKNCVFDGNDKVLDDSTLSRKVYEEGEREMGVSFEHGKYISNLRNIAKNEGLITPLEATVTGIIQDQTGVHGVKVNLPNNEKREYRAALTVVCDGIFSKYRKTLGESHVPTVNSGFIALKLKDADVPVKYTGHVILGGSFSPVLVYQITPTDTRMLCAYNGPKPPKDIKGYLQNEVLPNLPKELHPSVKEALKDQIKTHPNSYLPARQNDIPGLLVIGDALNMRHPLTGGGMSVGLGDTILVLKLFENIKNLADREAVNEALLEFHYERKHLDTVVNVLSIALFSLFSADSANLRILQKGCFRYFQRGGDCVSIPVGFLSGLLPRPYLLTWVFFKVAFYSIACNFGDNGLVLLPWSIIQIFTILFTAAAVFTPYLWKEFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3J2</accession>
    <name>A0A1E3P3J2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCA7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_62359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038981.1">
        <property type="nucleotide sequence ID" value="XM_019183348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3J2"/>
    <dbReference type="GeneID" id="30200594"/>
    <dbReference type="OrthoDB" id="10267123at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013243">
        <property type="entry name" value="SCA7_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08313">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCA7" evidence="1">
        <location>
            <begin position="155"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="316" mass="36578" checksum="95894E3BE9C403DF" modified="2017-01-18" version="1">MTLIPDTAALYHGDDDGVGIHQRSVNQQSDSMAPKLPFDVNDYLNFELNEDFISSSGDFINDYSTSDSNHSDSCFSVHQSSSKSLDGGFNQLTYEMDIFDKENYDYEHDYNDISRYGEFNDDDDTCYLKNYKLSSGAQPTLPSNDFKIFEYDHKDFNCSVITKLNNTPCKNKITCNFHSIPEKSKVERCDSVLKLIKNFKYLQKLNEDISKFTISSKSKSKSNGYTAASRMKRHNSFNDSSHIKKKPQLLNPIDILNDNDDYYQNEEDDDEFINTLILKIGLLKNDPQERLLEFQYKTELKNLATIEYYNQNQFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3Q3</accession>
    <name>A0A1E3P3Q3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_78181</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038739.1">
        <property type="nucleotide sequence ID" value="XM_019184677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3Q3"/>
    <dbReference type="STRING" id="683960.A0A1E3P3Q3"/>
    <dbReference type="GeneID" id="30201923"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="159"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="242" mass="27771" checksum="B86401A01A243C45" modified="2017-01-18" version="1">MQSVQRIRLPSVNDIFPKVENQLIMNQQCYNVQANMIQSSPIEMHQNYSLQQPQVTYTLAPQVSANLPQISQQQPATAGPIAQPIPYYPQNSIPIHHHPHQHLPPASITPSNSPINYINTGNNMGNTPPPYNYHQPRHNGVIMGSIINSQNKYKISKNSVTRQSNAWSANDDELLKYLKEVKNLGWREISMYFENRTANGCQFRWRRIVALSKQQENYQHTIKRTDLQNTESRSNSLESLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P456</accession>
    <name>A0A1E3P456_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_78698</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039285.1">
        <property type="nucleotide sequence ID" value="XM_019184775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P456"/>
    <dbReference type="STRING" id="683960.A0A1E3P456"/>
    <dbReference type="GeneID" id="30202021"/>
    <dbReference type="OrthoDB" id="6058203at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01446">
        <property type="entry name" value="DSP_MapKP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18533">
        <property type="entry name" value="PTP_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF561">
        <property type="entry name" value="PROTEIN TYROSINE PHOSPHATASE 36E, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="6">
        <location>
            <begin position="229"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="494"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="635"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="11"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="350"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="352"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="759" mass="86515" checksum="2299C404FB1D3614" modified="2017-01-18" version="1">MSILRSPLEQNSGFTFPDSSGPSNSSADAIKKDTNNNNNIIATSVKETFQIQQPSSYFASPESINHVSHSKFKHSRIGTIDENLMDTSNQFEKSFANLSLKKVSSRDTVNSESTLVESLHNKNDSLNSINSIDTLNSFRLKRFNSASSVPSFNAITSFGSPEITHHSTFPLLTSSPNGSLCSSASSSSASSSNSTFQLKKVAKFPIPSTISTMTNTELSLILEGMTSDEDNSILILDVRPYIDYNTKHIKNAINFNLPSTLLKRSNFTLTRCLSNLSIIENVKINKFLSDEEASDKKIIIYDDSSVTNNEISLAAYGTSNKFLNDQNFKGEIYVLKNGLINFNEEFPGLTEASSNSNGNANNHSTGHQPIKPPHNRSLSLANISSSTPISPNLSRFQLPKLPKTPVFKIRHNEEFYDFDNYKIINEFKFLNSTINRPDENIEKPLPKWLKTVLDKNNYLSLFEKFKNLEIEEKKRINNLVATNNVTSGIELGFKNRYKDIFPYEHSRVKLSLTPTQEHQGYINANFINCPQLTKLRYIATQAPLSETTKDFTKLCEDNDISLIISLTNQFENGIEKCFPYWDDSENFEILERTNLNDFVLRRLRLTKYNKEVLQIQILNWSDFDIMVENQQVDVLKLIYLKKTILDHLQKQDQNVIVHCSAGCGRTGTFCTLDSIINTKFDFSDMNNNNNKSLIDFDPIFEIVENFRVQRISMVQNLRQYLFIYDCLLNYYENFKDFRNDMFGELDNLNILKNYLSEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4E4</accession>
    <name>A0A1E3P4E4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_92761</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039576.1">
        <property type="nucleotide sequence ID" value="XM_019186377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4E4"/>
    <dbReference type="GeneID" id="30203623"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="52" mass="6188" checksum="51D85FA4FBBFE445" modified="2017-01-18" version="1">MRWILTIVTTHTFCKRCLLITTFDFDFEKEQSIDDHSWSSQLPKSVTITTEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4F2</accession>
    <name>A0A1E3P4F2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribulokinase/uridine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_62752</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039395.1">
        <property type="nucleotide sequence ID" value="XM_019183430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4F2"/>
    <dbReference type="STRING" id="683960.A0A1E3P4F2"/>
    <dbReference type="GeneID" id="30200676"/>
    <dbReference type="OrthoDB" id="738517at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="1">
        <location>
            <begin position="82"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="225" mass="25634" checksum="8A1C0EB95B8C2BA0" modified="2017-01-18" version="1">MSEENNTVVILVGGGHAAGKKTVCERIKSNFLENLNANIEIEIFDLDVYIKDQPEIPYGPTRFDCSKLLNEIELFEKKSDIKKLILVPGLYSLYDRRLRNIASMKLFIDCDADTRLTRWITAETLNGETKLEDLLDNYVNRARPEFNEFIQPTKEFADVILPNDEEINGISLICDGIQPLFNQSSLSKSGKTLYPRFGFDSESSSTLNLQAEAFNGQKDRFYDLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4F4</accession>
    <name>A0A1E3P4F4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial succinate-fumarate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_30885</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038979.1">
        <property type="nucleotide sequence ID" value="XM_019181776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4F4"/>
    <dbReference type="STRING" id="683960.A0A1E3P4F4"/>
    <dbReference type="GeneID" id="30199022"/>
    <dbReference type="OrthoDB" id="204711at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005469">
        <property type="term" value="F:succinate:fumarate antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000021">
        <property type="entry name" value="SFC1p Mitochondrial succinate-fumarate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049563">
        <property type="entry name" value="TXTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788">
        <property type="entry name" value="SUCCINATE/FUMARATE MITOCHONDRIAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788:SF2">
        <property type="entry name" value="SUCCINATE_FUMARATE MITOCHONDRIAL TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="8"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="108"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="213"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ59772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="35100" checksum="8A235AD405F6083F" modified="2017-01-18" version="1">MSDKKKSSSTAVNLIAGGTAGLFEALCCHPLDTIKVRMQLHRKSGIVKNPGFITTGISIAKKEGVTGLYKGLGAVVMGIIPKMAIRFSSYEFYRGLLKDPNTGVITTGSTFLAGVGAGITEACLVVNPMEVVKIRLQAQHHSMSDPLSVPKYRNAGHAVYVIIKEEGFKTLYRGVSLTAARQATNQGANFTVYSKLKEYLTEHHTQNGNNGVIPSWQTSCIGLISGAIGPFSNAPLDTIKTRLQKETKQASAAGEEIKQSALSRITRIGKDLIKEEGVGALYKGITPRVMRVAPGQAVTFTVYEIVRGWLEQLPALSNKEASAFDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4I9</accession>
    <name>A0A1E3P4I9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_68753</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039435.1">
        <property type="nucleotide sequence ID" value="XM_019184137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4I9"/>
    <dbReference type="STRING" id="683960.A0A1E3P4I9"/>
    <dbReference type="GeneID" id="30201383"/>
    <dbReference type="OrthoDB" id="2133190at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052099">
        <property type="entry name" value="Regulatory_TF_Diverse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019006">
        <property type="entry name" value="Sre1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336">
        <property type="entry name" value="TRANSCRIPTION FACTOR HMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR HMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09427">
        <property type="entry name" value="DUF2014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="173"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="678"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="266"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="678"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="698"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="864" mass="96999" checksum="4CDC2E7E97764339" modified="2017-01-18" version="1">MTDVFYSFDDTDALLASLSSSSANTLSPSDNSEFSGSAKSSSYGLSPEEVIYSDNWNNNFNGADADTLNDDFFGKDLINFDANELSNNDQQVDNNNTTKVKLENNYASTFVSPESMAEGDFNTVNNKLQPQVLKREESLDSDSLSENESPDFTSSSTIKKTSSTANKVTKPKKTKATHNVIEKKYRTNINTKITALRDSVPALRMAAGDANTTINDLDGLSPAAKVNKAIVLTKATEYIKHLERKNSLLMRENQQMKHYLGSLPAQSQQQHHIPQQQFHPQPYNMPPNPQYQTSPLQNNAQPSPDSFQPANYNGQQVPIANVQQPYQMGLPGKVLMGGMATMVGQNLFNGGGDYGDFRGLSAFPIMGNPLFIKFFHMIQFLFVSLSVVYLISPALFQKSQAPKDKKTKIATVEWYELAKELVFIQIGSKLKREATQGEVDLLINQALLSSEKHSFFSLTHLLLRLFTYQPSFEVSFGKLVVAKLLLSYTDIATFINLNGIISTSLSEIANQKVSDSNCRYFFKEFKTISAQNSEAFKRLLNIVIGLPIGTNCERGVEDKGYFLILRDERVLFDYKTLLISLRANEIFREVLLEYINLTFNSEEIAKLDNEELREGKKELWNKLNLAENLAPKRSIVDIRIKLFKSILNEKYVDSVLQLVNEESKAVIATKKLNGEVVEEKLSSEEETSKPPSPILKDENEDEVYDSDESFGDSETEEEDEVEIAQPAPNPEVVESTPSSSINPKFQNLLSQDLFNALVSSSILKFAHADQHEQVSKLLKYIKFSKDELTLLSFISLYKLVQKFPKNWLEGKEGEVIENLVGHLRVWVGDSSNVNFNDLKEVGLHLKREISDNLVEVGKSFSELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4J7</accession>
    <name>A0A1E3P4J7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoribosyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_78750</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039341.1">
        <property type="nucleotide sequence ID" value="XM_019184782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4J7"/>
    <dbReference type="STRING" id="683960.A0A1E3P4J7"/>
    <dbReference type="GeneID" id="30202028"/>
    <dbReference type="OrthoDB" id="9973266at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032263">
        <property type="term" value="P:GMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046100">
        <property type="term" value="P:hypoxanthine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032265">
        <property type="term" value="P:XMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000033">
        <property type="entry name" value="Xanthine phosphoribosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363">
        <property type="entry name" value="HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363:SF1">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="3">
        <location>
            <begin position="18"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="216" mass="24431" checksum="8D7A9A9C0F6B15F1" modified="2017-01-18" version="1">MTDNQDIEDSKVYISYNHVHQLIQEAAPRLKNFNPDLIICVAGGGLIPARQVRTFLKEPGSPNVRIMAVVLSLYEDIGSVSTQAEKLGKQVVRTQWLDYINTDFTLLGKNILIVDEVDDTRTTLNYAVTELKKDVEEQAKAKGIDVKDAGTNFGIFVLHNKLKPKKADLPPEVYNEKNYIAARDIEDVWIAYPWESNDIVYHTKRAVEQGNDVFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4L7</accession>
    <name>A0A1E3P4L7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Luciferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_100398</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the NtaA/SnaA/DszA monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039361.1">
        <property type="nucleotide sequence ID" value="XM_019180293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4L7"/>
    <dbReference type="STRING" id="683960.A0A1E3P4L7"/>
    <dbReference type="GeneID" id="30197539"/>
    <dbReference type="OrthoDB" id="5561043at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051260">
        <property type="entry name" value="Diverse_substr_monoxygenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016215">
        <property type="entry name" value="NTA_MOA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03860">
        <property type="entry name" value="FMN_nitrolo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011">
        <property type="entry name" value="ALKANESULFONATE MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011:SF16">
        <property type="entry name" value="C2H2 FINGER DOMAIN TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000337">
        <property type="entry name" value="NTA_MOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="6">
        <location>
            <begin position="42"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="490" mass="55385" checksum="A584B57F0E44A03B" modified="2017-01-18" version="1">MSGPATKKQQTTKPKKTLILNAFENAASGHQFPGLWKHPKDRSRSYKDLDYWIYLAKLLERGKFHSVFIGDALTIYDDYKGPNNFGPAASSGLNVPKNEPSAPVTAMAAVTENLSFGLTFSTISEHPYHFARRLATLDHLTKGRVGWNIVSSYLNSTARQLLNGEPLPDHDERYVKTEEYVQVVYKLFLSSWRDDAVKLDLDKGIFSDPDLIREINHVGKYFNVPGPSISEPQRLPVLVQAGTSTKGKHFAAQHAEAVYTNGLTPENLREKIDGIRDLAEHYGRDRYDIKVLSSAIVITGETEEEAQAKYEELKKYADPDGAQVLFGGWTGIDLSQWDDDQELKYVKNNGMRTYVENLTKFNPGKTFTKKTIVERISINGSTSATFVGTPDQVADKIEEFVEISDVDGFNFTYVVWPETFENLVDLVIPKLQAKGLAQTEYTVENGTYRENLYFQKGQSFVPQSHPAYNLRWTDKDSQEEFEAKLSKTYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4N9</accession>
    <name>A0A1E3P4N9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_62812</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039457.1">
        <property type="nucleotide sequence ID" value="XM_019183443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4N9"/>
    <dbReference type="STRING" id="683960.A0A1E3P4N9"/>
    <dbReference type="GeneID" id="30200689"/>
    <dbReference type="OrthoDB" id="4703at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052416">
        <property type="entry name" value="GTF3C_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15052:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15052">
        <property type="entry name" value="RNA POLYMERASE III TRANSCRIPTION INITIATION FACTOR COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="587" mass="65664" checksum="A1EE50994B1F125D" modified="2017-01-18" version="1">MPPRARRNTKRIDYSKFADPSAQNGVDSDGDEEVKDADLHESDNDDDDVLEDGDDEPIDEDVTEDETIEEEIPKAKAKSKKAPKTKKSTTPQPENGNNTPVKEIRLTKHITNLKEKFYRFYGANEDKLVEVLKLRRAWESSMFDFPSSQLKEYIPSVTIKDVPKAKFTELSKEQFNEKFPLERSINFGYNENPSETIAPGGIAPNQRAQVINTGGLVTDIAWLSHGSEAAEQILAVAVSDISDTSIAPEFSLLGYKNYKSGFLIYSLDTQTYTLTLIKAVIHNWGNSWDLQWKNHRGMGVLSAVFNDGKVRLIHFDYEDEEDVSYTEITEPSFEYELPNMKITSYDWLGDKIICGTESGYVAEFVLGDKVPSYVHHIQSGYIFSIISAESKYDETMILTSCADGSCALMSTKDFQSTKVITPKTKSISKSIAYSRQLYSFVQLDAQYSAKLTLLRSLFVVTSVVKHDGSTESIATSNLHPMLLSGGADGKVKITNLMRRILNGQKQTLSNHKILTLWELQYSANEDLYRLVDNLKVETLSSNDNTNVSNIYPPGVSINSIKWNENENAGKWYAAGSTSGLVIVGSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4Y7</accession>
    <name>A0A1E3P4Y7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_78988</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039595.1">
        <property type="nucleotide sequence ID" value="XM_019184818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4Y7"/>
    <dbReference type="GeneID" id="30202064"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133669" evidence="2">
        <location>
            <begin position="18"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="243" mass="24838" checksum="9AC8C9405E5FD24B" modified="2017-01-18" version="1">MLLKNIILASLIPLIAAQANGALDQDAGNNGRRTSAAEVASAGTTEATQANTKQESAATVKSEATEATQQSQAIVKTESQATQNTLQTTAQSQPTAANTDQSTTVWWTPSQSVWWTPTQTVWWTPSTSAEVSTIVSGSSTYLTTLATSVRSGSSESSSGSASNSKSANGKLTTEKDVVLTTVSFTSGSSTGAYTSSYTTSSIHTLNSNSSSGANALLVQGGSVKDSLKIVVPVCVAAGALIFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P527</accession>
    <name>A0A1E3P527_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome assembly protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Notchless protein homolog 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Ribosome biogenesis factor RSA4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_62906</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NLE1/RSA4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P527"/>
    <dbReference type="STRING" id="683960.A0A1E3P527"/>
    <dbReference type="GeneID" id="30200704"/>
    <dbReference type="OrthoDB" id="10267436at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110136">
        <property type="term" value="P:protein-RNA complex remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000092">
        <property type="entry name" value="notchless protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012972">
        <property type="entry name" value="NLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF0">
        <property type="entry name" value="NOTCHLESS PROTEIN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NLE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NLE" evidence="11">
        <location>
            <begin position="32"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="135"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="178"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="220"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="269"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="392"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="434"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="476"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00061016"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00068030"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF08154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ60344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="509" mass="56100" checksum="996BD7EE84C0C977" modified="2017-01-18" version="1">MATLIPPPSKKQKREAQKPREVDLVPADLPNVLIKFQASDTGESVGGSIRVPGNISEQQLEQLLNQLIGESDEPVPYSFSLLNDATIGDLVDIKDNLYSSVLKPGIKTTEDFMTLVYTPRAIFKVKPVTRSSASIAGHTSTILCTQFAPHTSSKMVTGAGDSTARIWDCETQTPKHTLKGHTNWVLCVAWSPDGEKIATGSMDNTIRLWDENGNSLGDALRGHTKWITSLSWEPYHLVKPGDSPRLASGSKDGTVKIWDTARRVPLMTLSGHSSSVSCVKWGGSGVIYSASHDKTIKVWDAKDGKVINTLKSHAHWVNHLSLSTDYALRMGPFDHTGVKPANQKEGIQKAKQNYEKVAKVNGVIDERVVTASDDFTMYLWEPLKSTKPICRMTGHQKLVNHVAFSPDGRNIVSASFDNSIKLWDGKTGKFDSTFRGHVAAVYQTAWSSDCRLLVSCSKDTTLKVWDIRTKKLSVDLPGHQDEVFSVDWSVDGKRVASGGKDKHVRLWTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5D1</accession>
    <name>A0A1E3P5D1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Deacetylase sirtuin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_83011</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class I subfamily.</text>
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    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039858.1">
        <property type="nucleotide sequence ID" value="XM_019185475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5D1"/>
    <dbReference type="STRING" id="683960.A0A1E3P5D1"/>
    <dbReference type="GeneID" id="30202721"/>
    <dbReference type="OrthoDB" id="2919105at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099115">
        <property type="term" value="C:chromosome, subtelomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="C:mating-type region heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005721">
        <property type="term" value="C:pericentric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140765">
        <property type="term" value="F:histone H3K56 deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140861">
        <property type="term" value="P:DNA repair-dependent chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009299">
        <property type="term" value="P:mRNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031508">
        <property type="term" value="P:pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046459">
        <property type="term" value="P:short-chain fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF15">
        <property type="entry name" value="NAD-DEPENDENT HISTONE DEACETYLASE HST4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="7">
        <location>
            <begin position="77"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="369"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="423" mass="47448" checksum="2CF1CA55CDEE7CBD" modified="2017-01-18" version="1">MEMITPPSSQTYVKLLRREVILPLTPPSERVLQQEDEKKGIKTKAKRKPKLKYRPPNGTVLNISTFDDSVSIIEQDDNIDYEDVSFLHYAIKHSKKVTVVTGAGISVASGIPDFRSSNGLFQGLKSATSSGSTGKALFDSNVYRDEESTAKFHAMIRQLHTLTEESEATDFHHMLNNISEEGRLLRLYTQNIDCLETSLPNLQTTVPIKSKPPYPPTIQLHGTIKYMYCSKCRWNSEIQPEIFNSNQAPACPECTELDEIRTIAGKRSQGIGCLRPRIVLYNEFHPDGEHIGQVSTLDLKSKPDCLIIAGTSLKIPGVRKLVRELARAVHAAKGCVIWMNTDEPSISIVDFVEYIDMIVVGDCQDIPKVKKEESDKSSRPVKSPKKQSRVTKPNPQTKKSKSKKDTKIQSRLEELVTKINEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5K7</accession>
    <name>A0A1E3P5K7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">5-hydroxyisourate hydrolase</fullName>
            <shortName evidence="7">HIU hydrolase</shortName>
            <shortName evidence="7">HIUHase</shortName>
            <ecNumber evidence="7">3.5.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_83071</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23736"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
            <dbReference type="ChEBI" id="CHEBI:58639"/>
            <dbReference type="EC" id="3.5.2.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4 7">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the transthyretin family. 5-hydroxyisourate hydrolase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.17" evidence="7"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039943.1">
        <property type="nucleotide sequence ID" value="XM_019185484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5K7"/>
    <dbReference type="STRING" id="683960.A0A1E3P5K7"/>
    <dbReference type="GeneID" id="30202730"/>
    <dbReference type="OrthoDB" id="10265230at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033971">
        <property type="term" value="F:hydroxyisourate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05822">
        <property type="entry name" value="TLP_HIUase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.180">
        <property type="entry name" value="Transthyretin/hydroxyisourate hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014306">
        <property type="entry name" value="Hydroxyisourate_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023418">
        <property type="entry name" value="Thyroxine_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023416">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036817">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02962">
        <property type="entry name" value="hdxy_isourate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10395:SF7">
        <property type="entry name" value="5-HYDROXYISOURATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10395">
        <property type="entry name" value="URICASE AND TRANSTHYRETIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00576">
        <property type="entry name" value="Transthyretin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49472">
        <property type="entry name" value="Transthyretin (synonym: prealbumin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00768">
        <property type="entry name" value="TRANSTHYRETIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transthyretin/hydroxyisourate hydrolase" evidence="8">
        <location>
            <begin position="4"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="141" mass="16090" checksum="D729E22DA6EF2850" modified="2017-01-18" version="1">MPPITCHILDTTRGKPAADVVVAIYRISELPMENSDEITITENLPFGLAKTNKDGRVSEWIFNPKDNLESLGIKGQEWNTLKPGIYKIRFQTSKYFKQLISDARTFFPFVDIMFNVSNPPDAHYHIPLLLANHSYTTYRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P685</accession>
    <name>A0A1E3P685_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_15351</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040138.1">
        <property type="nucleotide sequence ID" value="XM_019180643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P685"/>
    <dbReference type="STRING" id="683960.A0A1E3P685"/>
    <dbReference type="GeneID" id="30197889"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF81">
        <property type="entry name" value="PROTEIN ABHD13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="83"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="281" mass="31794" checksum="1E8CF6B179EC9EE2" modified="2017-01-18" version="1" fragment="single">KMFLATTASVTTASLLLLYKYQLKLIYPSGLNDARAPGAFHSTPQEYEIPYEDLYLETEDGVKLHAFLCLQDPKSPDYQNKTVLILNPNAGNIGLAVPTVYNFYQLGFNVFIYDYRGYGLSTGESTEIGLKKDTETVMEYINEHDQLNKSSVILYGRSLGGAVAIHIASKNYKLVKGVILENTFLSLRKNIPHVFPFLKYVAILCHQLWDSETAISKIDPNVKMLFLGAELDEIVSPDHMKKLYELANVGDRYEKFVLFQGFHHNDTTLAPGYWDIISDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P694</accession>
    <name>A0A1E3P694_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein mL59 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_61494</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040143.1">
        <property type="nucleotide sequence ID" value="XM_019183187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P694"/>
    <dbReference type="STRING" id="683960.A0A1E3P694"/>
    <dbReference type="GeneID" id="30200433"/>
    <dbReference type="OrthoDB" id="18529at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037507">
        <property type="entry name" value="Ribosomal_mL59"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040922">
        <property type="entry name" value="Ribosomal_mL59_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28041">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28041:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18126">
        <property type="entry name" value="Mitoc_mL59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Large ribosomal subunit protein mL59" evidence="1">
        <location>
            <begin position="25"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="170" mass="19956" checksum="A0289B7571A54BD6" modified="2017-01-18" version="1">MRVSLGLFNTAVKTEISYFNTLPEKLQRFFTRYPPRPFKEYASESTLTTAENANPFIANKHPITKRYHTAKYSMRRQSDLWKIAYRHGIQDSLPPLANGKKFYQEKYDTKPLMKGVLRPKGAKYERERPLKEAKMKEALLHADEKIAELRGKKKIENRLAKKAKKVKHFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6G9</accession>
    <name>A0A1E3P6G9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">snRNP core protein D1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_28918</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the snRNP core protein family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040061.1">
        <property type="nucleotide sequence ID" value="XM_019181552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6G9"/>
    <dbReference type="STRING" id="683960.A0A1E3P6G9"/>
    <dbReference type="GeneID" id="30198798"/>
    <dbReference type="OrthoDB" id="9626941at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036261">
        <property type="term" value="P:7-methylguanosine cap hypermethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000016">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="12">
        <location>
            <begin position="2"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="86"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ60854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="112" mass="12762" checksum="E8F045AA1EB35177" modified="2017-01-18" version="1">MKLVRFLMKLNNETVQIELKNQTIIQGTIISVSPNMNIILKKIKMVIKNRDPQLLDYINIRGNMIRDVILPDSLNLDLLLTESNLTTSSSSSKKRKVEDSNRGGVKRVKRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7B8</accession>
    <name>A0A1E3P7B8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Checkpoint protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_28560</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HUS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040465.1">
        <property type="nucleotide sequence ID" value="XM_019181504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7B8"/>
    <dbReference type="STRING" id="683960.A0A1E3P7B8"/>
    <dbReference type="GeneID" id="30198750"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044778">
        <property type="term" value="P:meiotic DNA integrity checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016580">
        <property type="entry name" value="HUS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007150">
        <property type="entry name" value="HUS1/Mec3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12900:SF0">
        <property type="entry name" value="CHECKPOINT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12900">
        <property type="entry name" value="MITOTIC AND DNA DAMAGE CHECKPOINT PROTEIN HUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04005">
        <property type="entry name" value="Hus1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011312">
        <property type="entry name" value="Cell_cycle_HUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="264" mass="30337" checksum="B08E679F842F5F0A" modified="2017-01-18" version="1" fragment="single">TLGAIIGLRKYCVLRFTPTHLNIISASLSEPQVWCKLSNELFDVYEVESLRENTISFEMNVDQLFHVLKQFEKSNSDQLAIRLQKRENDSGKRSASLAVFFDDTISTTSVVTHTFSISVRLLRKESDERITEPELSNVDVLMKLPVDISMLFRRIERYKNLEFLKIIGTKNGSLSLNIQEENNLNVTITWNELLQVQQNSDSLSGDESFEIMVKLRDWKLGAKICEICKNMVLIISKDEALVLHCYLDDSETCEIIYFINGVNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7C7</accession>
    <name>A0A1E3P7C7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_99809</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040500.1">
        <property type="nucleotide sequence ID" value="XM_019186698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7C7"/>
    <dbReference type="STRING" id="683960.A0A1E3P7C7"/>
    <dbReference type="GeneID" id="30203944"/>
    <dbReference type="OrthoDB" id="347657at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000196">
        <property type="term" value="P:cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05581">
        <property type="entry name" value="STKc_PDK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000128">
        <property type="entry name" value="Serine/threonine-protein kinase ksg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000534">
        <property type="entry name" value="Serine/threonine-protein kinase PKH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039046">
        <property type="entry name" value="PDPK1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057614">
        <property type="entry name" value="PH_PKH3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF163">
        <property type="entry name" value="3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25347">
        <property type="entry name" value="PH_PKH3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="17"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="322"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="441"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="696"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="332"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="383"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="472"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="501"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="706"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="783"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ61293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="815" mass="91135" checksum="CFD69A226BB21350" modified="2017-01-18" version="1">MATTTSTTTRKRSVQDFQFGNRIGEGSYSTVYKAVDIQSKKVYAIKVLSKKHIVKEKKIKYVNIEKTTLNRLGRHPGVVTLYYTFQDDASLYFVIDMAEFGELLTIIRKLGSLSEECSKYYMIQIIDAVDFMHKKGIIHRDLKPENILVNSDMKLMITDFGAAKVLDLDDQGNIIDSNATEESFAKGSFVGTAEYVSPELLKYNKCGFECDIWAIGCILYQFLVGVPPFKGKTEYLTFEKIVNLQYQWPNFYIPSTIKDLVDSLLKTNPFDRLTIPQIQQHKWFGNFNWEDKNRIWVTNPPKLEPYQPIIQQHNENSQQLKKILNPPQPPALTSSRPRSPNSPLSRPLQQAPQPQLQRVHSPQAMPQQRIVQPKPPVPQQQQSYHQVSPIKQQIPQQSNFQQKPSAPAFVQAIKQQVPPQIKSQQPTPQLAPALVPMSSQVKPQTIVQQTQKPSRPPPQQRSTSQPSKIQPPLTTRPSPAQKSNSFSPTKPPAIRPGTNRSSPSSTSSPKSPLRSSSSNGTSGKLPLDISSKLSQCENIIKLDYINESELIYSSLYTFDPSSPLSNLKKNLGLDDVSINEIIMKNEKLLKDDSKRVVMMITDDGNLYLFKSSTLKKFEFYLKIDLTDKMFAMYDYEFSEFSESGYLILEISKIDKLIFLAPLSKEVKEDLREISNIHIGLNLSWIEALLKTKEFLKSKPSSPPVQSPASSQPMTKSSSTTSVTTAPTSSTSTVKKSQSATTASTTSSSSKVKVSNGVNSNGGSKTMNSKTKPPQAKPPKRPTSKSPTITTQQQIQQQNRKFAGGAAAAAFNAATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7M7</accession>
    <name>A0A1E3P7M7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar import and degradation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_17147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GID4/VID24 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040636.1">
        <property type="nucleotide sequence ID" value="XM_019180727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7M7"/>
    <dbReference type="STRING" id="683960.A0A1E3P7M7"/>
    <dbReference type="GeneID" id="30197973"/>
    <dbReference type="OrthoDB" id="62at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045721">
        <property type="term" value="P:negative regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007039">
        <property type="term" value="P:protein catabolic process in the vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018618">
        <property type="entry name" value="GID4/10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14534:SF3">
        <property type="entry name" value="GID COMPLEX SUBUNIT 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14534">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09783">
        <property type="entry name" value="Vac_ImportDeg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00061469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="23616" checksum="A337D97913219DD0" modified="2017-01-18" version="1" fragment="single">SSTTLTDFFIKNQIKPQTTSILRPGAIFVGSQQSGRSTYEVEVELKSVDLSKSYLSGYLKIQGLTESHPEIITFFESEIISENYSFFTDNKDWGSTDKIDIQHWTKFQNWRNSLPVKKILDKNYVHKNFLKHNHIYMRWKERFLVPDAKVENIDGASFAGFYYISFNQKTGNISGLYFHKSSEKFQQLELSYVPSLGVGGSYQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7N7</accession>
    <name>A0A1E3P7N7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_90698</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040517.1">
        <property type="nucleotide sequence ID" value="XM_019186254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7N7"/>
    <dbReference type="GeneID" id="30203500"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="59" mass="6474" checksum="5D00CABDFBBD37F9" modified="2017-01-18" version="1">MSDPLDRLVMISQHVHNQKINKSTIPRGMPEIPANIQTNFSRPVPGGQVKSPTAIKPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7Q1</accession>
    <name>A0A1E3P7Q1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_61887</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040537.1">
        <property type="nucleotide sequence ID" value="XM_019183267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7Q1"/>
    <dbReference type="GeneID" id="30200513"/>
    <dbReference type="OrthoDB" id="3979708at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05325">
        <property type="entry name" value="carb_red_sniffer_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051468">
        <property type="entry name" value="Fungal_SecMetab_SDRs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544:SF7">
        <property type="entry name" value="NADB-LER2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="254" mass="27675" checksum="ACFC86B6BEBC04ED" modified="2017-01-18" version="1">MSGKVYFVSGGNRGIGFQFVKILSSNQENTVIASARDPVKATELQALADTQKNVKIVKLDVSDKASVDALDVQLKEVAKDGIDVLIANAGISQSVLPAIDTAEDIYLRHYRTNVLGPIFLTKALYPYLKLKETRHLTYVSSLAGSIGGFIPFTSSAYGQSKAALNYSIKEISFELGAEGFTAVAMQPGLVDTDMSRAGLKALKEVNPDVVEILKDYPNIPAEESAKSQLENIIFKLNKDMNGKFFDYHGVEQVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7S6</accession>
    <name>A0A1E3P7S6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Glycosyltransferase family 71 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_83218</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040134.1">
        <property type="nucleotide sequence ID" value="XM_019185507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7S6"/>
    <dbReference type="STRING" id="683960.A0A1E3P7S6"/>
    <dbReference type="GeneID" id="30202753"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000033">
        <property type="term" value="F:alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392:SF1">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="690" mass="80306" checksum="E00D96CE634DA5C7" modified="2017-01-18" version="1">MINQALRYLQSLRHLVSNFSFNKRLINLNVFIFVLILIIIAQSLYSYVYSTNYDSILTKYEPKTFNNFPFEKKCNLYFDHLHKSNHDWILNNFDTKGYDDNYITFQDFLEFKKTAEKNMVFDEAKILELEKEFNERSFDSIKVDQRIIDAVTTLRIFGKCFIEVDDSKKPGFISSIFEDNKKQGEDEKEAEKQNKLQQDLCHDTEARIFPWLSNILPTYTRWNGEVSQGLPDLSNTQSNYDDNEEFHSHGKAHDKRENVRTNCFLKSFKNAMNGKGIVISASDGQKDELMRLALILRATDNKLPIQVMHKGDLSEKNQKELIHQFRRPIKLENLPLSFERLALHQAPKFPPQDLWFVNVENSINKDYKQYFKKYANKLLAYLFNSFEDMILIDTDTVPFVELESILKFPGFVEKGAFFFKDRELRGSNTQNQVNYFNRLFPSKLDNFFFQIPLVTDFTLKNRFIGDLRNHYMESGVVAIKRTTHFTGMLSAVQLNFWSITARKIHGDKELFWLGQSIAGNENYEFNRLGAASVGELTPRENKLFPDSLANELCGNHPGHVNGYDNQTLLWINSGFTFCKNTDAAGFDAGKPLFKQYDGDSLREHYKAVTKIRSAIIPPPQEIEGKNDKGNPTLSWSDAHKYCFSYTWCAYDGVGEMSNDIERGFLVNFDDQQVKTFDFFGDLWMKGVRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7W7</accession>
    <name>A0A1E3P7W7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Defect at low temperature protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">DLT1</name>
        <name evidence="9" type="ORF">WICANDRAFT_78129</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Required for growth under high-pressure and low-temperature conditions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Membrane</location>
            <topology evidence="2 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DLT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040712.1">
        <property type="nucleotide sequence ID" value="XM_019184669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7W7"/>
    <dbReference type="STRING" id="683960.A0A1E3P7W7"/>
    <dbReference type="GeneID" id="30201915"/>
    <dbReference type="OrthoDB" id="4096362at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038869">
        <property type="entry name" value="DLT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40021">
        <property type="entry name" value="DEFECT AT LOW TEMPERATURE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40021:SF1">
        <property type="entry name" value="DEFECT AT LOW TEMPERATURE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="357" mass="40068" checksum="5E5B2ABAB10F5B5B" modified="2017-01-18" version="1">MPTQPIGGRLINNPKIRRWLYRSSLIFFIAFFAGFCAVVPIDSIVQASQSSNYALNTFVVVGALVLFGVTGIVLTTTRIYMQRSALQDIPKRYVPLDDGDLPKECDVMIKENFANCEKIRSKALNTDDDDEEVVKHPGLSGPKSELLPPSLAFDEVVRAVGDKLKWGGALMGSDTRVPPNSSFREIVAYLEEHLIATDYKLNKEYVELYEVLRFSGDLISEENFIKFMTLSIEFVKITRLNMADESQLERIRRQYSESEASLNAPGLNGSEFYSKLSLPGALLSRSANHTNNGSTTASISQVDTNWLTNSRPTLQLNEFQEPAQSEQEYQRYHDMLRRVGTGQTVLSFRSGKGPEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P814</accession>
    <name>A0A1E3P814_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_86392</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S5A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040758.1">
        <property type="nucleotide sequence ID" value="XM_019186042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P814"/>
    <dbReference type="STRING" id="683960.A0A1E3P814"/>
    <dbReference type="GeneID" id="30203288"/>
    <dbReference type="OrthoDB" id="1731724at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01452">
        <property type="entry name" value="VWA_26S_proteasome_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000005">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027040">
        <property type="entry name" value="PSMD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10223">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10223:SF0">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="4">
        <location>
            <begin position="5"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="193"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="230"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="193"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="230"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="268"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="281" mass="30641" checksum="FF56F214AB857646" modified="2017-01-18" version="1">MVLEATMIVIDNSEFTRNGDYLTNRFEAQLDAVDLIFQAKTNSNPENTVGLLAAAGNGPQVLSTLTADFGKLLSGIHETKIEGSLHFATGIQVAALALKHRQNKVQHQRIVAFVGSPIQESEKELEKLAKKMKKNNIAIDFINYGEESINTSKLEKFVSIVNSHDNSHLVTVPPSDRLLYEVVGSSPIVIGEDGGNFNAGGETGGDGFDFGSDPNMDPDLALALRLSLEEEQARQAREQQTRTETQAPLESINESTREEPSNSNDNENNNDGDTNMEDAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P818</accession>
    <name>A0A1E3P818_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14 17">Homoserine dehydrogenase</fullName>
            <shortName evidence="14">HDH</shortName>
            <ecNumber evidence="5 14 17">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">WICANDRAFT_75758</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of L-aspartate-beta-semialdehyde (L-Asa) to L-homoserine (L-Hse), the third step in the biosynthesis of amino acids that derive from aspartate (the aspartate family of amino acids), including methioinine and threonine, the latter of which is a precursor to isoleucine; production of homoserine leads to a branch-point in the pathway as it can either be O-phosphorylated for processing to threonine, or O-acylated for processing to methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-homoserine + NADP(+) = L-aspartate 4-semialdehyde + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15761"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.1.1.3"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:15763"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:25213"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 17">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 14 18">Belongs to the homoserine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="5 14 17"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040756.1">
        <property type="nucleotide sequence ID" value="XM_019184254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P818"/>
    <dbReference type="SMR" id="A0A1E3P818"/>
    <dbReference type="STRING" id="683960.A0A1E3P818"/>
    <dbReference type="GeneID" id="30201500"/>
    <dbReference type="OrthoDB" id="67851at2759"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009090">
        <property type="term" value="P:homoserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000006">
        <property type="entry name" value="Bifunctional aspartokinase/homoserine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011147">
        <property type="entry name" value="Bifunc_Aspkin/hSer_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019811">
        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022697">
        <property type="entry name" value="HDH_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43070:SF5">
        <property type="entry name" value="HOMOSERINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036497">
        <property type="entry name" value="HDH_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14 17" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 14 17" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="9 14 16" id="KW-0521">NADP</keyword>
    <keyword evidence="10 14 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14 17" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Aspartate/homoserine dehydrogenase NAD-binding" evidence="20">
        <location>
            <begin position="11"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Homoserine dehydrogenase catalytic" evidence="19">
        <location>
            <begin position="151"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>L-homoserine</name>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR036497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ61549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="361" mass="38705" checksum="FE2889DF8D3AB1AB" modified="2017-01-18" version="1">MVKSVNLAIIGTGVVGSAFLNQLKALKSQISYNVILIARSAGSLISKDYSPIDITNWEATVEKATDSNLSTEALLDFLKKSPLPAILVDNTSNGEIAASYSQLVKNGVSVATPNKKGFSSDLASWKELFNNAQGENAGLVYHEATVGAGLPIIGTINDLVQTGDEVEKIEGIFSGTLSYIFNEYSTAEPKDVKFSEIVKVAKELGYTEPDPRDDLNGLDVARKVTIVARLSGSEVESPTSFPVQSLIPKDLESVKSAAEFLEKLPNYDHELQSLKDEAAKENKVLRFVGKVDIPNKIVSVGVEKYDYSHPFAALKGSDNVISIKTKRYQNPLIVQGAGAGADVTAAGVLADTIKIAQRIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8D3</accession>
    <name>A0A1E3P8D3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Endonuclease</fullName>
            <ecNumber evidence="17">3.1.30.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">WICANDRAFT_59756</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the DNA/RNA non-specific endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.30.-" evidence="17"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040880.1">
        <property type="nucleotide sequence ID" value="XM_019182868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8D3"/>
    <dbReference type="STRING" id="683960.A0A1E3P8D3"/>
    <dbReference type="GeneID" id="30200114"/>
    <dbReference type="OrthoDB" id="5418055at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004529">
        <property type="term" value="F:DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00091">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.570.10:FF:000004">
        <property type="entry name" value="Nuclease 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.570.10">
        <property type="entry name" value="Extracellular Endonuclease, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018524">
        <property type="entry name" value="DNA/RNA_endonuclease_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044929">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001604">
        <property type="entry name" value="Endo_G_ENPP1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020821">
        <property type="entry name" value="ENPP1-3/EXOG-like_nuc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040255">
        <property type="entry name" value="Non-specific_endonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13966:SF5">
        <property type="entry name" value="ENDONUCLEASE G, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13966">
        <property type="entry name" value="ENDONUCLEASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01223">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00892">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00477">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01070">
        <property type="entry name" value="NUCLEASE_NON_SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 17" id="KW-0540">Nuclease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA non-specific endonuclease/pyrophosphatase/phosphodiesterase" evidence="19">
        <location>
            <begin position="78"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="ENPP1-3/EXOG-like endonuclease/phosphodiesterase" evidence="18">
        <location>
            <begin position="79"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR640255-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR640255-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU366055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ61673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="330" mass="36686" checksum="692156ACD9BB5224" modified="2017-01-18" version="1">MSKYLASGALGAGVSVAIANFFWGKPSSTSTASPVDSSSGYGVPAQQGQSGGLVSGPVVNPSEFFKYGFPGPIHDLQNRQEFVSCYDRQKRNPYWVVEHITPESIKSNGTVDRKKSVFKEDEKIPATFKAQLRDFFRSGYDRGHQAPAANAKFNQTAMDETFYLTNISPQVGEGFNRDYWAHLEFFCRDLTKTYGSVRIVTGPLYLPKRDPDGKWRVTYEVIGNPPNVAVPTHFFKLIVAENPNRNPGDQSLAVSAFVLPNAPISNQTPLRSFEMPIEALEKSSGLTFLQNVPENKKKQLCREVDCSIVVREFNNSLPPKKDMLLLPPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8T7</accession>
    <name>A0A1E3P8T7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_81951</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040927.1">
        <property type="nucleotide sequence ID" value="XM_019185313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8T7"/>
    <dbReference type="STRING" id="683960.A0A1E3P8T7"/>
    <dbReference type="GeneID" id="30202559"/>
    <dbReference type="OrthoDB" id="18110at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23524:SF1">
        <property type="entry name" value="MRH DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23524">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="258"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="302"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="336"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="360"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="396"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="420"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="3">
        <location>
            <begin position="255"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="466" mass="50452" checksum="0E3671CEF9A9172C" modified="2017-01-18" version="1">MVFMSSTQSFFLTDVLGISTKVGDYIGTLGFADELMSISLSPFLGALSDKIGAKHIQVCGVFIVGLSFFILTLAKNVYPDMLLIRLFFAIGATASGSMMTALLAELSSSDLEYYDPIEQHEPQEINEEEANKAHSKRNGKLTSVIGISSGLGALFAVSAYLPLPNKFGQREPPAQALKHSYFVVGAIALTSSIFLSYGLFSSKNFKIPFLSKYLSPYDDVLDDIEEEEHEVHLEQQKHTYFGLLKLGFSEAKDPKIALAYLGGFLSRSTTIVSAVFLPLYVNKYYYKEGICSTADRSSCREAYIQAAILTGIVHMVSLIFAPIFGFTADKFGRKNSMIISCIFGIVSSFGFAFSKSPKSAAAVVFSCFLGASQIGIILTSMSLCTDKKRAHNGSISGVYSLVGGIGILIISKVGGYSADFWIGAPFIILGGFQIITIVASLYAGGKLDFLLSKFSARLQLPEDEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8Z2</accession>
    <name>A0A1E3P8Z2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetylglucosamine-induced protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_102701</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041045.1">
        <property type="nucleotide sequence ID" value="XM_019180363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8Z2"/>
    <dbReference type="STRING" id="683960.A0A1E3P8Z2"/>
    <dbReference type="GeneID" id="30197609"/>
    <dbReference type="OrthoDB" id="10053431at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022036">
        <property type="entry name" value="DUF3605"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35020">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-INDUCED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35020:SF2">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-INDUCED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12239">
        <property type="entry name" value="DUF3605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="24809" checksum="AAB9C990D9CAAC72" modified="2017-01-18" version="1">MNPVNQDTSIVREEPISWEEVCQIIKTNHLERLKRSKSKLAEYKRFKDYLKENNISSTAILLGDDLQWAKVDSNHQILETYLEARSDKLLRDPRDLKIIENRFPYNFESNISHLLVWTKVPIESDPTSPRGDISTYTRKLINKYIWNTFVKGLGIPESNIIWFRNWHALQSIRELSHIHVLIKDLSPEQLSRIKGTPGVPLSDDDYIELAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P940</accession>
    <name>A0A1E3P940_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_76108</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041133.1">
        <property type="nucleotide sequence ID" value="XM_019184324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P940"/>
    <dbReference type="GeneID" id="30201570"/>
    <dbReference type="OrthoDB" id="39175at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF7">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="286" mass="33108" checksum="8B3F31DB7FBD84FF" modified="2017-01-18" version="1">MANTRAKKITKHSKKFERTQTGCLQCRQKKWVLIQNLRIIKTLTNIFERKKCDGKRPSCESCIKRGISCYFPANIRIFQYQKKQNKEFVSEPTPKSLAKSDADSKIEPINFKQVKLEHPTCNTEQDKRRPSVDDELKENLLSLSDKIEQILEVKEEPDIESIQLQSRSDSIASLISDSDNDSIISMNQPRTPPSTSTYEISESQRATNLIHEGGSINKILNQMTIDLIKKSINNLQYIDFEKKRDLSILQAQDILEEEQYNKSLGYIDEVMPDELIFHPDEYLSFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9W8</accession>
    <name>A0A1E3P9W8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">asparaginase</fullName>
            <ecNumber evidence="2">3.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_82299</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-asparagine + H2O = L-aspartate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="EC" id="3.5.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the asparaginase 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.1" evidence="2"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041416.1">
        <property type="nucleotide sequence ID" value="XM_019185363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9W8"/>
    <dbReference type="STRING" id="683960.A0A1E3P9W8"/>
    <dbReference type="GeneID" id="30202609"/>
    <dbReference type="OrthoDB" id="542841at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006530">
        <property type="term" value="P:L-asparagine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08964">
        <property type="entry name" value="L-asparaginase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1170:FF:000001">
        <property type="entry name" value="L-asparaginase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004550">
        <property type="entry name" value="AsnASE_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00520">
        <property type="entry name" value="asnASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500176">
        <property type="entry name" value="L_ASNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133755" description="asparaginase" evidence="10">
        <location>
            <begin position="22"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="L-asparaginase N-terminal" evidence="11">
        <location>
            <begin position="41"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="12">
        <location>
            <begin position="255"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="active site" description="O-isoaspartyl threonine intermediate" evidence="5">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="128"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ62209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="368" mass="39591" checksum="D9E7ADD890316654" modified="2017-01-18" version="1">MLLSKTILASILLSLITSTQAVPLLKREDSPVPKNASLPTVKIFGTGGTIASRGGSGSQTAGYELGLTVADLVESIPDLAKAANIEFLQVSNVGSSSVNYTHLIPLYHNVSQSLLNDEIDGAVVTHGTDTLEETAFFLDLTIKTEKPICVVGAMRPATAISADGSMNLYQAVSIAGSEEAKGRGTLIPFNDRINSAFWATKTNANTVDTFKAVEQGFLGTFLNNDVEFFFPPVRPNGYHYFDISNVTDASQIPEVVILYSYQGLDPELVRYAVEERGAKGIVLAGSGAGSWTATGYTVLEELMAKYNIPIVRSRRTMDGSVPVTAPLKEYISSGYLNPQKSRIMLQLCLYSGYNEEQIRSVFSGVYGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9Z5</accession>
    <name>A0A1E3P9Z5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Condensin complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_49607</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the CND2 (condensin subunit 2) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041440.1">
        <property type="nucleotide sequence ID" value="XM_019182634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9Z5"/>
    <dbReference type="STRING" id="683960.A0A1E3P9Z5"/>
    <dbReference type="GeneID" id="30199880"/>
    <dbReference type="OrthoDB" id="362021at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070058">
        <property type="term" value="P:tRNA gene clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022816">
        <property type="entry name" value="Condensin_barren_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108:SF9">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05786">
        <property type="entry name" value="Cnd2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017126">
        <property type="entry name" value="Condensin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="8 11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="138"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR017126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ62233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="690" mass="78467" checksum="B0A231041D665E0C" modified="2017-01-18" version="1">MAYVQSKVVKKRLQEKRRVSGASLLRDELNNDSGNDSLLDGLPAFQQNKGVMMANFEEWIKMATDNKINTTNSWNFALIDYFHDLNVLREKDNNINFQKASATLDGCVKIYSSRVDSVATETGRLLSGLAARKQMEAEKAKEAAIEEGDEEDGDDTNAEYEDGDQQQTKKKKSRASKTKELTDTLVSFDSIRVKKLSQELSIDPLFKKTLAEFDEGGAKSLLLNTLNMDNHTRVVFDATTGDQQQEDKIEEGERDEHEERDIEMSGMDEEDIDISVFKDFIFQDESDMDSLLLCPSFGHLESVLKDVKKAKSILNDVNQQNISIPGDDEHTNAGPGNNDMDFDFDFGGDYDDDGSFGNLDQEAAAVLFNDETHAENEDDTRVVANVVDHDLMAYFDHTLKRNWAGPEHWKVKQLKKDKAQQLQPLLNGTDKKRKPKEEFKIDFMEDMDEDEEEKLFAKSKVATTLSAEQQESTDSHLLPDDLYFSSEQLVRLFLKPDQKLKIFQKRVKSDDTINHQPQPKDEQEVADANFWADNYKEQETRVDDEPSFYGGNDDYDFGYDAGDFGDDDIGKGDSNETKLDFGTQLITGGNKVKPEYVNYSKTAKNVNVKYLKDNLWQSLEGKKLDTEQQDEKQFSDVIKDIGKLYPKEEKKDLSTSFCFICLLHLANEHSLTLESNDQLTDLQIKGLKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA09</accession>
    <name>A0A1E3PA09_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nucleolar complex protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_25957</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP14 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041450.1">
        <property type="nucleotide sequence ID" value="XM_019181212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA09"/>
    <dbReference type="STRING" id="683960.A0A1E3PA09"/>
    <dbReference type="GeneID" id="30198458"/>
    <dbReference type="OrthoDB" id="441771at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007276">
        <property type="entry name" value="Nop14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183">
        <property type="entry name" value="NOP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04147">
        <property type="entry name" value="Nop14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="98"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="137"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="259"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="167"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="267"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="279"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="333"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="370"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="804" mass="92497" checksum="8098579888FA8D60" modified="2017-01-18" version="1">MAGSQLKQLKAALKENGLTGQTNVKRKGKGSKRAPSETRRDDREEKIQRIREQFNPFEIKTNKTKYETNGKFVKGAQGKPGISKQIGEDQRKAAWEAKKAAKNKSGLLKDRRFGENDASLNPEEKMLQRFTHERLKQSNRANLYNLDDDSDEEGGLTHMGKSLALQDDFDAEDLENSDDDDFMKPKKRSFDDEGLAEEDEDESAVPKKKTKTEVMKEVMAKSKYYKAQRQMAQEQRENQVEELDDEYADVLHELGTIPKSKPAAVQASKTPNGINYEQSVRELNMDRRAAPADRTKTEEELKKEWEDKQKELEQARLRRMQGDDYGAEKGPDELDDDFWGEESEDQAEGVETDGSDAEVEEESESESESTGRAFTGSKNKVSCPQTLEDLHSQLESVALKGTPAHIKKIIDTYSPRLQAGNKEKLAVFTNVLFQHILFLSESDDVDSDDFPEIQESLISTLKKLSERFPNELTEFLREQIKEMQERINETIAGGDEYPMVSDLVFFGLVGMLFSTSDHYHLLVTPSLILMGESLEQLKYNSLNLLIAGTFISDTILKYQRIAKRYIPEVTFFLQKALLSLSPKEIKHSSITTKPDVRLPLKKGITTEEQTLRISDVDRELQESHEEALLKKIISVIDYALDTWKEKTAFIEIAQPFSVILAKYQKFYPEFQKITQLVDKFNRLLKFANDERKPLELQTHKKLAIATYAPKFEENFNPEKRSYDHDRSRQETNKLRHLLKQEQKIALREIRKDSRFEARQQIKEKKDSQDAYHSKMARILNSISTVEGAEKNAYDREKKARKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA95</accession>
    <name>A0A1E3PA95_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein SDA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_25183</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for 60S pre-ribosomal subunits export to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the SDA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041351.1">
        <property type="nucleotide sequence ID" value="XM_019181114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA95"/>
    <dbReference type="STRING" id="683960.A0A1E3PA95"/>
    <dbReference type="GeneID" id="30198360"/>
    <dbReference type="OrthoDB" id="2196187at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007089">
        <property type="term" value="P:traversing start control point of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027312">
        <property type="entry name" value="Sda1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048292">
        <property type="entry name" value="SDA1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007949">
        <property type="entry name" value="SDA1_MD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012977">
        <property type="entry name" value="SDA1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12730">
        <property type="entry name" value="HSDA/SDA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12730:SF0">
        <property type="entry name" value="PROTEIN SDA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21638">
        <property type="entry name" value="SDA1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05285">
        <property type="entry name" value="SDA1_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08158">
        <property type="entry name" value="SDA1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SDA1 N-terminal" evidence="9">
        <location>
            <begin position="64"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="SDA1 middle" evidence="8">
        <location>
            <begin position="539"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="SDA1 C-terminal" evidence="10">
        <location>
            <begin position="675"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="546"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="648"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="550"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="563"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="648"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="725" mass="81538" checksum="214BDEBC817EC385" modified="2017-01-18" version="1">MVKKRGAAILPTNIILLQNLVRRDPESYREEFLQQYAHYESLRDIFIASQASHDATEFAELIGFVSAVCSCFPRETANFPDELKSLITNNHRDLTPDLREKIIQCLTMLRNKDIITAESLIQTIFPLLTAYSSGTNVAGTNVKALRHQVYSTLITLLKSVNTGAKNQKLNRSTQALLFNLLEQKDSQGIWATRLTRELWRRGIWDDSRTVEIMTQATLHPDSKVAYSGVKFFLGADKERDEAMEDESDDEGFDMTALRHKMQINKKTSKRGKKLETAVKTMKKKNNSKHSATYLNFSAIHLLRDPQGFADSLFSTHLQGKSSNKFDLEQKIALMNLLSRLIGTHKLTVLGIYSFFLKYLTPKQRDVTQIMAAAAQASHDLVPPESISVVVRKIADEFVSDGVAPEVAAAGINTIREILSRAPLAIDVTLLQDLTEYKGSKSKPVNIAAKSLISLYREVAPEMLKRKDRGKTASMEVQAGEKTGLPQFGADTGVQGIPGLELLAQWRQEQGLAANDGEDDEANWEVDDEEIDDEDIEGEWVTIESDKEYDVNSDLDLSDDEGESKEQSVEPATEEAKEEAISKIVKLDPEAAFQELASTRILTPADFAKIEELRVQAGVEKIMGSKMRNEEHVDSSSLVGPVKYKQLREERIAQAREGRDEREKFGSRRGQRDAPHSTTNREKARKKNFVMMIHKRDVQGKAKRSLRDKQKVLQAHITKQKKGRHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAA9</accession>
    <name>A0A1E3PAA9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WDR5-like beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_48870</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041024.1">
        <property type="nucleotide sequence ID" value="XM_019182619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAA9"/>
    <dbReference type="STRING" id="683960.A0A1E3PAA9"/>
    <dbReference type="GeneID" id="30199865"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059122">
        <property type="entry name" value="Beta-prop_WDR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050349">
        <property type="entry name" value="WD_LIS1/nudF_dynein_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44129">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25175">
        <property type="entry name" value="Beta-prop_WDR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="18"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="WDR5-like beta-propeller" evidence="4">
        <location>
            <begin position="19"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="60"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="102"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="144"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="190"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="327" mass="36294" checksum="DD23C68813A444BD" modified="2017-01-18" version="1">MADGQVKDDLYKIRYKISSQHNKESTTIKISPNGLYFATASSDATIHIFNLESGSFIRSLSSHSRGISDIAWAQDSRHLASVSDDMTIRIWDVEFGECLNILEGHTYHVTCVEFNYRGNLLVTGSADEAIRIWDAQNGKCLKTLSAHSDPIASVDLSWDGTIIASASYDGLIRLFDTESGQCLKTLIYDKGGSSFPVSYIRFSPNGNYLLATTLDNTIRLWDYMNNRVVKTYQGGTGFSEKYTCGSRFVTTSKDPLIASGSENGNIYIWDLQSKKILSTIEISPSSPVLQIDTFGKVLCAISMDGTVKVFDLSDEFKEYDSDRTDTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAN0</accession>
    <name>A0A1E3PAN0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_25796</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041683.1">
        <property type="nucleotide sequence ID" value="XM_019181192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAN0"/>
    <dbReference type="STRING" id="683960.A0A1E3PAN0"/>
    <dbReference type="GeneID" id="30198438"/>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF22">
        <property type="entry name" value="DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION-REGULATED KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="56"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="365" mass="42178" checksum="265952025FF8B14C" modified="2017-01-18" version="1">MSSYEKAEILKNHEVYFIGHKRSKNSNLRDFSGNFGFDDGEKNLIVETGDHINYRYQVISKLGKGMFGNVMKCYDHKTGTDVSIKIMKNDITWSLQCINEIKILKKMKHENLLAYYGHFNFRSHICISTELLGVNLFEALEATKFNGFDISIIRKWSKGLLSGLDFIHKQGFVHADLKPENIMLVDSYSLDIKIIDFGSSSAIGDVSYPYIQSRYYRAPEILLGCRYNEKIDIWSFIALIFELYHGDPLFGARDESQLFSMIVKLIGMPTPDTILTMRNEVLKYGSINKKGDPKLVDKRVFIFTKFDEKGKYKGLRIDQSPTDKNYLKDLKETQPQFVDFLNSALIWNYKERPSAQTLLNHKFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAR9</accession>
    <name>A0A1E3PAR9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nitroreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_88118</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nitroreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041710.1">
        <property type="nucleotide sequence ID" value="XM_019186137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAR9"/>
    <dbReference type="STRING" id="683960.A0A1E3PAR9"/>
    <dbReference type="GeneID" id="30203383"/>
    <dbReference type="OrthoDB" id="2138173at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.109.10:FF:000001">
        <property type="entry name" value="Nitroreductase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="entry name" value="NADH Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033877">
        <property type="entry name" value="Frm2/Hbn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43035">
        <property type="entry name" value="FATTY ACID REPRESSION MUTANT PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43035:SF1">
        <property type="entry name" value="FATTY ACID REPRESSION MUTANT PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="FMN-dependent nitroreductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="7">
        <location>
            <begin position="8"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="191" mass="21218" checksum="CC10D4ACE64B765F" modified="2017-01-18" version="1">MSQFLKTITSRRTHYALQNTLPEGVTVDQVQSIVQQIVKHTPTSFNSQTVRAVIVTGELHKQIWGSVADAIENESGKKRPISAKDEAYGSIIFFDEEETVKKFQTDFAIYAPYFPIWATTANGAAQINTWAALQEIGLGGHLQHYNGYVQAALGDRVPKSWSVQAQIVFGTPVSGPYEKTFIDNEVKVLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB09</accession>
    <name>A0A1E3PB09_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_66846</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041806.1">
        <property type="nucleotide sequence ID" value="XM_019184059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB09"/>
    <dbReference type="STRING" id="683960.A0A1E3PB09"/>
    <dbReference type="GeneID" id="30201305"/>
    <dbReference type="OrthoDB" id="28208at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05471">
        <property type="entry name" value="pepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034164">
        <property type="entry name" value="Pepsin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF75">
        <property type="entry name" value="ENDOPEPTIDASE (CTSD), PUTATIVE (AFU_ORTHOLOGUE AFUA_4G07040)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133762" description="Peptidase A1 domain-containing protein" evidence="8">
        <location>
            <begin position="18"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="9">
        <location>
            <begin position="97"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="455"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="455"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="126"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ62599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="481" mass="51704" checksum="2876113F1186E9E8" modified="2017-01-18" version="1">MLSFHILLLFFSVSIFAIIPIGEDRFISTKSYKVSSVQLPPQGFENYLQLLKASPQSKGLNIQKDSDNNNNGSSNSTVLNLKPGITTLQTDENNECYFVNTTIGKKEFPLIIDTGSAYLWVYGDNCTDSACQNNALYTPASSESSTSTFALAYVTGTASGQVVEDNIIVNKLATTQKFKFGKADKVPDFFKNYPVSGIFGLPSNDSSAIQSIISALYDSHAISIEKFSISLGNISNSTDYPNSGIFAIGDPIKELYKGDIHYSDLIENDSHYWLIPIESVTLDSFDVNFTNPVNLGDGQANSSTSRKAIVDSGTTVLALPKQDALDLHSYFTNSITDGTNFAILCNSSFNIGLNINGHDWTIEPENYLGAKYSDPSMAGYCVSNIQGIDSAAADSWILGAAFLKSVYAVFDVNNQQIGFATKNEDVVLTSTSTSPSSSAIQTSSAAYYGNSSSIMTSLSSNSSASTSASKTSHKAAEQYGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB43</accession>
    <name>A0A1E3PB43_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_60642</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041795.1">
        <property type="nucleotide sequence ID" value="XM_019183030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB43"/>
    <dbReference type="STRING" id="683960.A0A1E3PB43"/>
    <dbReference type="GeneID" id="30200276"/>
    <dbReference type="OrthoDB" id="6159137at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="126"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="219"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="385"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="301"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="503"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="301"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="312"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="674" mass="75712" checksum="C0BCC15AF86E5DDE" modified="2017-01-18" version="1">MSISKEEFNKAEQEAQAKAAEEAVKENDQVDDTTVTKPSTRSSEDNHQFHSPLNSNAIITISTDEPIDIQPLLKTHETFELLPSVPPFIKIKFIDKNEATNLKDEILLKYPDLEINVEYDALTHPGNIYVRGLLPEITTQDLYKIFEPFGEITSLKIVNDEFGRSKGYGFINFVDGDSADEAITKLNGLLVNGSKLYLNHHIAKKERLERMDFEKSNFTNLYIKNLPIDYTLELFNQLFEKFGEINSTFLPELPEHELNNPTSKRFGFVNFKNHESAIAAIEELNNKEISPNHLLSVSRAQRRDERNDHHNNNVNSFRNNSISPTPAPLIFQSPPPIISTNPIPTALPPNLITSPQLQHPISATPSQGTTPIIPLPLPGPNHQQSNLYVKNLSPTIDDNALHSAFAPFGIIVSAKVMTTEDGSSRGFGFVCFRTSPEASRALLAMNGNVLDGNILHVSFAQKNSRRKFQSNPPHQQRMEYPYYYNQYQTAEFAPMYYGGNGNAPPGGAPPPPQTPGHLATGLPNGNNGYIPYNAVRYSNNGNGFNKYNGFKNYNNGFNRVQQQQKQQSSDERINSEYSKKIGKLTSEISSIGEDNDDVKIEKISKFLIIPKLLENGQIENVELKAKDFLQRQVDDYKQQQQDDGADSSDVYLDIINDWANDENSLIQSLEKLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB94</accession>
    <name>A0A1E3PB94_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 25">tRNA-dihydrouridine(47) synthase [NAD(P)(+)]</fullName>
            <ecNumber evidence="4 25">1.3.1.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">tRNA-dihydrouridine synthase 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">WICANDRAFT_25221</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U47 in cytoplasmic tRNAs. Catalyzes the synthesis of dihydrouridine in some mRNAs, thereby affecting their translation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>5,6-dihydrouridine(47) in tRNA + NAD(+) = uridine(47) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53364"/>
            <dbReference type="Rhea" id="RHEA-COMP:13539"/>
            <dbReference type="Rhea" id="RHEA-COMP:13540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.89"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="20">
            <dbReference type="Rhea" id="RHEA:53366"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>5,6-dihydrouridine(47) in tRNA + NADP(+) = uridine(47) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53360"/>
            <dbReference type="Rhea" id="RHEA-COMP:13539"/>
            <dbReference type="Rhea" id="RHEA-COMP:13540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.89"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="23">
            <dbReference type="Rhea" id="RHEA:53362"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a 5,6-dihydrouridine in mRNA + NAD(+) = a uridine in mRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69851"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:69853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a 5,6-dihydrouridine in mRNA + NADP(+) = a uridine in mRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="22">
            <dbReference type="Rhea" id="RHEA:69857"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 25">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="25">Belongs to the dus family. Dus3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.89" evidence="4 25"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041855.1">
        <property type="nucleotide sequence ID" value="XM_019181119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB94"/>
    <dbReference type="STRING" id="683960.A0A1E3PB94"/>
    <dbReference type="GeneID" id="30198365"/>
    <dbReference type="OrthoDB" id="259935at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106414">
        <property type="term" value="F:mRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102265">
        <property type="term" value="F:tRNA-dihydrouridine47 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000145">
        <property type="entry name" value="tRNA-dihydrouridine(47) synthase [NAD(P)(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45846">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(47) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45846:SF1">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(47) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25585">
        <property type="entry name" value="zf-CCCH_DUS3L"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="26" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 25" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 25" id="KW-0288">FMN</keyword>
    <keyword evidence="11 24 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="17 25" id="KW-0520">NAD</keyword>
    <keyword evidence="15 25" id="KW-0521">NADP</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16 25" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 25" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="14 24 25" id="KW-0862">Zinc</keyword>
    <keyword evidence="13 24 25" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="28">
        <location>
            <begin position="97"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="28">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="24">
        <location>
            <begin position="97"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="24">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="26">
        <location>
            <begin position="429"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="18"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="27">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00045934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00049513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU291113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="ODQ62648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="616" mass="70023" checksum="3809C9F517C65CEC" modified="2017-01-18" version="1">MSEETKRPGSPENTGAPELKKPKTSAEVESVPRETRGIAHVKKEYIVDSRPVEVIDDDSAEAAGDRNDDGGNGKGKGKGKGKKQRGQNKNREIKQNQEKVQLCQSLVDPELEKVCKFGAETCRFTHDLGVYLEAKEKDIEGVCPVFNALGYCPAGMKCRFLHSHYDGEGKKLIKDLTKYEEAKLSNHELNHITNEEKLSLIKKKFDFPLSDAVVKIMDDIQEDNKDNANEYVETRFFAAEKKKLDLVGKKIVSPLTTVGNLPYRRLMRTLGADVTYSEMALTLPLIQGTNSEWALPKAHCSEYPGFGVQIATSKHWQGAKSAEIVSKLCPSVSELNLNSGCPIDLLYRQGSGSGLLDQPARLIRILNSMNYCSGEIPTTVKIRMGTRDNHPIAHNLVKRLVKETQTAAITLHGRSRQQRYTKEANWDYIAEVGQVLKETEQELEDDKDRRDFQKINFVGNGDCYNFNDWYKAVENPYIDSVMVARGALIKPWIFEEIEAQQYLDKSSIERLEIVKTYADFAMQHWGTDEYGIALSRRFLCEFLSFFHRYIPYGILEKYPIKLNERPENWKGRDDLETLLGSNDYKDWIKISEMFLGPAGENFNFTPKHKSSSYEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB95</accession>
    <name>A0A1E3PB95_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_50406</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome b5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041890.1">
        <property type="nucleotide sequence ID" value="XM_019182651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB95"/>
    <dbReference type="STRING" id="683960.A0A1E3PB95"/>
    <dbReference type="GeneID" id="30199897"/>
    <dbReference type="OrthoDB" id="432299at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004128">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000001">
        <property type="entry name" value="Cytochrome b5 reductase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051872">
        <property type="entry name" value="Cytochrome_b5/Flavoprotein_Rdt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237:SF1">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="6">
        <location>
            <begin position="87"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="164" mass="18409" checksum="E3FB82A4B10680ED" modified="2017-01-18" version="1">MTSSNTTTTSQNLRRIPVIQPPNSSQQRARSQLAPPTTASFPSLNSAQRAGGARKKVVLEQGCSPLDWARLNSSGQNLRGIEPHQFPLKVDKEMLKLHKQKNDCWTVLKGKVYNITPYVKFHPGGVEEIMKCAGKDGTSLFNKYHAWVNFERMLENCFIGFYIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB96</accession>
    <name>A0A1E3PB96_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">P-type Na(+) transporter</fullName>
            <ecNumber evidence="19">7.2.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">WICANDRAFT_99077</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>K(+)(in) + ATP + H2O = K(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75815"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>Na(+)(in) + ATP + H2O = Na(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:14634"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IID subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.3" evidence="19"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041886.1">
        <property type="nucleotide sequence ID" value="XM_019186679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB96"/>
    <dbReference type="STRING" id="683960.A0A1E3PB96"/>
    <dbReference type="GeneID" id="30203925"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008554">
        <property type="term" value="F:P-type sodium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000016">
        <property type="entry name" value="Calcium-transporting P-type ATPase putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000015">
        <property type="entry name" value="Sodium ion P-type ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000039">
        <property type="entry name" value="Sodium P-type ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000047">
        <property type="entry name" value="Sodium P-type ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006414">
        <property type="entry name" value="P-type_ATPase_IID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01523">
        <property type="entry name" value="ATPase-IID_K-Na"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="17" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="291"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="748"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="785"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="830"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="873"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="924"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="959"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="23">
        <location>
            <begin position="2"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00035017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00035029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="ODQ62679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1016" mass="112212" checksum="A328FBD90CD2E88D" modified="2017-01-18" version="1">MSSFDERKLNHSATIGLTEQAAEALLKEHGENTLGDDQKISLPKIIIHQSCNAMILVLIISMAITLGIKDWISGGVIAFVVGINVGIGSLQEFKASKTMNSLKSLSSPTARVLRNGELKTVPTKYVVPGDIVSVKVGDTIPADLRIVDCLNFETDEALLTGESLPVAKEYEVVYEDIIGVGDRLNMAYSSSVVTKGRATGIAVKTGLHTEIGEIAQSLKSQKKKLNTDDDTSFFKNVLVLIKDGIGSFLGTTVGTPLSRKLSKLAIMLFFVAVVFAIIVMATQKFKVSSEVAIYAICVAVSMIPSSLVVVLTITMSVGAKIMVKRHVIIRKLDSLEALGSINDICSDKTGTLTQGKMLVKAVWVPSGGTYNMIDTNDPLDPTVGTMKYHTKSPLEASQEEKSNDDVEVDSENEYFQKWLLCATLANIATVFKDEHGDWKAHGDPTEIAIQVFAHRNNVPRTSLTKGDDPKYVHLNEFPFDSSIKKMSAIYSESGNEKENLVFTKGAVERVLTSCKDWYVDGKLQQLTAEDHEVIEKNMNTLSGDGLRVLAFATKTIPKDHDIKTRETIESDLTFLGLVGIYDPPRLESLGAVKKCHKAGINVHMLTGDHPGTARTIAQEVGILPHNLYHYAQDVVDSMVMTAQQFDDLTDAQIDQLPVLPLVIARCSPKTKVRMIDALHRRKKFCAMTGDGVNDSPSLKKADVGIAMGIAGSDVAKDASDIVLSDDNFASIINGIEEGRRMNDNIQKFVLQLLAENVAQALYLMIGLVFKDENGLSVFPLSPVEVLWIIVVTSCFPAMGLGVEKAQPDIMQKDPKPSKESVFSWEMLVDLFVYGVWMAICCLCCFIICIYYKGDGELGIDCNSKYSDSCYYVFRSRSASFATMTWCALILAWEVIDMRRSLFAMQPETETPYTQVFKDLWGNQFLFWSVIGGFLSAFPVVYIPVINKKVFLHGPIAEEWGIAFALTVAFWIGCEVYKVFKRLYFKKQIVHNPEHDLEKKNPFEKYTSFSRAATMQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBJ2</accession>
    <name>A0A1E3PBJ2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Helicase-associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_60842</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042000.1">
        <property type="nucleotide sequence ID" value="XM_019183065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBJ2"/>
    <dbReference type="STRING" id="683960.A0A1E3PBJ2"/>
    <dbReference type="GeneID" id="30200311"/>
    <dbReference type="OrthoDB" id="3980792at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF99">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="591" mass="68147" checksum="1B0F690422B14391" modified="2017-01-18" version="1">MGKLDLIEENSQLIFHSIENNDFTFLVSTGVVPLLQFISKIENTKYLFVSDDTSLATRFKNVPYQTSVYDSRFSVVGSSWVTYDLCIYLISEDVRFFELFDFIILDGIDDREPESEYLFSLMLNLKMIGKYIIPLKSNFKTELYTGFVGNFGYTSNVLKLKEEPSKSVYYLPSDPSNLNKEIIKTINDILVIEKSLEQCDILVMLSSFNEIQVFKTQLVDADIKHDFSILTDLSAYKTIGAHRRVILATDSKILRDVDHRIRYIVDSGVMKLKKFDECGLESFSTRFTNRNQILQHLSILEHGNSSYFGMFVEDKIVDFEYGINDCFLVQLLYFLKIGLDVEKFEFLKSPMEQVIKSAINLSIYLGLVKIEDGSEIKLTELGSTVMKFQVELEISSLSLFIALSISNRFNSTNELLKISSLLLSCNVDSLSRDLKNFNHHKTRTHDVPQSDFLTLLNIFDTMIENQQHIKQSSQVFRDSVMSKEAKKQILHCFKQLRSKLNQPVNFERAIEDDIQKCLFSGFQLHASTVLQNVSNPGTIELEPILSKRPDRLNEMDKVKIILTSDLYENNDIIIYHSREYFFSLLLLTFAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBM7</accession>
    <name>A0A1E3PBM7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HAD-superfamily hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_60354</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041494.1">
        <property type="nucleotide sequence ID" value="XM_019182974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBM7"/>
    <dbReference type="STRING" id="683960.A0A1E3PBM7"/>
    <dbReference type="GeneID" id="30200220"/>
    <dbReference type="OrthoDB" id="10251048at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006353">
        <property type="entry name" value="HAD-SF_hydro_IIA_CECR5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01456">
        <property type="entry name" value="CECR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF93">
        <property type="entry name" value="FI11325P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="39588" checksum="59962B38CBCC700F" modified="2017-01-18" version="1">MFTSTFKRQLIPTFSRALHSSSALRTKAHEAGFVFDMDGVLVKGKKAIPKAKEALQLLDERNIAWILMTNGGGVSEAQRAEFLSEELDLDIDIDQIVQSHTPLVTLPNKSEQKVLVIGGDGDKSRKVAENYGFGLALTPADYIKTTPAIWPFHRKELLEFGRNLPGIDDLKIDSILVFNDPRDLGTDLQVIFDVLKRDPNTPVIFSNNDWLWANEYKEPRFGQGVIRFIIEQMYKEIHGKEANRTILGKPTKVAYDYAHHILINRSIHLNQISDIPKSSLTLGEEIKGSHIKQVFMVGDNPASDIIGGYNYGWNTALVRTGVYRDGDQLPCKPTVIADDVYGAVHQGIKHLGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBT9</accession>
    <name>A0A1E3PBT9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Glycosyltransferase family 32 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_26380</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042096.1">
        <property type="nucleotide sequence ID" value="XM_019181255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBT9"/>
    <dbReference type="STRING" id="683960.A0A1E3PBT9"/>
    <dbReference type="GeneID" id="30198501"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.20:FF:000002">
        <property type="entry name" value="Initiation-specific alpha-1,6-mannosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039367">
        <property type="entry name" value="Och1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834:SF11">
        <property type="entry name" value="GLYCOSYLTRANSFERASE HOC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="313" mass="36576" checksum="8E7F9824E8BBB0DC" modified="2017-01-18" version="1">MKQQDEKINNLEKNRKELEKKIQELRRPPIDSTLREKLAFLFPYDQSRKFPAYIWQSWKYGLNDERFGEKFKEGELQWAVKNPGFVHELFNDDTSSAIIHHLYVLIPEIVEAYEVLPSIILKMDFFRYLILLARGGIYADVDTLPLQPVPNWIPENVSPNEIGMIIGIESDPDTPDWRKTYARRLQFCQWVIQAKPGHPILREIVAKITELTLQKKKQGDLEINDNLDIMKWTGAGIWTDVVFTYFNDYVQSGIYSKITWKDFTKLEVPKLISDVLVLPITSFSPGIGTMGAKDEDHPLAFVKHYFENIWKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBW1</accession>
    <name>A0A1E3PBW1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Replication factor C subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Activator 1 41 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_26492</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042116.1">
        <property type="nucleotide sequence ID" value="XM_019181270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBW1"/>
    <dbReference type="STRING" id="683960.A0A1E3PBW1"/>
    <dbReference type="GeneID" id="30198516"/>
    <dbReference type="OrthoDB" id="4199794at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902983">
        <property type="term" value="P:DNA strand elongation involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000011">
        <property type="entry name" value="Replication factor C subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000032">
        <property type="entry name" value="Replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000237">
        <property type="entry name" value="replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF20">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="58"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00075373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="358" mass="40401" checksum="9A0035BDFC9505D4" modified="2017-01-18" version="1">MSVNSFFAKNSNKESSKPKEITERALPWVEKYRPKNLDEVTAQDHAVTVLKRTLKSANLPHMLFYGPPGTGKTSTILALTRELYGPSLSKTRVLELNASDERGISIVREKVKNFARLTVSNPSAEDLENYPCPPYKIIILDEADSMTADAQSALRRTMETYSGVTRFCLICNYITRIIGPLASRCSKFRFKPLDNQDALSRLQYIQKEENIRLEEGVLEEVLKIASGDLRKAITFLQSAARLHSSLMTTDKDDLITVKSIREIAGSVPRDILEGLLKVSETKEFEKIFKVVDDAISEGWSGVEIVNQLHDELILNDAYSPEQKNQFSQILFETDQRLSNGTDEHLQILNLVTKISKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC08</accession>
    <name>A0A1E3PC08_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_82465</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041624.1">
        <property type="nucleotide sequence ID" value="XM_019185382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC08"/>
    <dbReference type="STRING" id="683960.A0A1E3PC08"/>
    <dbReference type="GeneID" id="30202628"/>
    <dbReference type="OrthoDB" id="4068661at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
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        <property type="entry name" value="RRM"/>
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        <location>
            <begin position="148"/>
            <end position="223"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="92"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="121"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="243" mass="26587" checksum="F2B3B72A23680892" modified="2017-01-18" version="1">MAPRIGKRSAKNSSSNPLAQRLDSTKIKPSSSSSKDNNNNNNKRKPYDSNPLLARLSEIGNRKSSGSNNNSNENPLAQRIRNGKFVPPSITSQTQALQNQRLRKSAPSKTPEGKITKRSKSASNNNNNNIRNGSSKTVLKIRNASNPNFLKIKNLEYGTNIRDLKVVLESIGKTKSIKIKDLESGSALAEVIFVSEATLEKAHTQLNGAIADGRTLRTEITSDSEIFNGAVDSYPNGPRSMRR</sequence>
</entry>
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    <name>A0A1E3PC15_WICAA</name>
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            <fullName evidence="4">Altered inheritance of mitochondria protein 11</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="primary">AIM11</name>
        <name evidence="5" type="ORF">WICANDRAFT_25621</name>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AIM11 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="AIM11"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
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            <end position="47"/>
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    </feature>
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        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="163" mass="18370" checksum="BD3C69D58F1267C7" modified="2017-01-18" version="1">MSAFKQGLELPKAVIERNEEYRQRRRKQMIFFFATTAVTLVSSRLAYRGVQTRRYVPGLFNANHVPPPFSFHRDAISAIAYSSVLATSSFAMAIAGVSWTWDVSTVKEFTYKLKTKLGGDEKEKELSEAKLDEESLSLQDAINSFLNGEGFEEEEATKLADSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC17</accession>
    <name>A0A1E3PC17_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dolichol kinase</fullName>
            <ecNumber evidence="3">2.7.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_27543</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polyprenol kinase family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ62512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019181384.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3PC17"/>
    <dbReference type="GeneID" id="30198630"/>
    <dbReference type="OrthoDB" id="377083at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004168">
        <property type="term" value="F:dolichol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043048">
        <property type="term" value="P:dolichyl monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032974">
        <property type="entry name" value="Polypren_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13205:SF15">
        <property type="entry name" value="DOLICHOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13205">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="447" mass="49997" checksum="841A70BE97896520" modified="2017-01-18" version="1">MESEKDSNLLISAACGIIALVSAVIIAFRVQTNNAKKEGSTEPIPLPPWNIIYLIFLPSFLALTLNKDLLLYNVALTSTLLNLPFFAKILVQMSILGSNSDHTDYIVNFKVTAGHLAINYVLGQISEYKSLDKVEVNLFSILLTDLYLIDSDSLYVLILQKLFISFGAGIVLIYLITKTYNENNWLRTILLMCSWTGTFVGLTMYQLDPILGKNSIVWIYEYAAATPTRIQILSIWLVSLILLIPTIFNYKIQLSPNFRRKIWHFLVLGLIVYPLHLDAEFVKVSLAGSLILFLVVEQIRYLKLYPFGVTLDKNLRLFADFRDDKGPIIISYIYLFIGISLPILFNNSVVGLVVLGVGDSLASIIGSNYGTINWGRSQKTVEGTMTFVTASFAVCAALKQIGWFFQNKTFHSLLLTCTLSGILEGNSNLNDNILIPGFMSIVLEILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC71</accession>
    <name>A0A1E3PC71_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MIF4G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_82897</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042220.1">
        <property type="nucleotide sequence ID" value="XM_019185458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC71"/>
    <dbReference type="STRING" id="683960.A0A1E3PC71"/>
    <dbReference type="GeneID" id="30202704"/>
    <dbReference type="OrthoDB" id="10252707at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005846">
        <property type="term" value="C:nuclear cap binding complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000339">
        <property type="term" value="F:RNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042789">
        <property type="term" value="P:mRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027159">
        <property type="entry name" value="CBP80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015172">
        <property type="entry name" value="MIF4G-like_typ-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015174">
        <property type="entry name" value="MIF4G-like_typ-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412">
        <property type="entry name" value="CAP BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412:SF2">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09088">
        <property type="entry name" value="MIF4G_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09090">
        <property type="entry name" value="MIF4G_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIF4G-like type 1" evidence="1">
        <location>
            <begin position="342"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G-like type 2" evidence="2">
        <location>
            <begin position="587"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ63013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="850" mass="97589" checksum="D68FC70F90482EA3" modified="2017-01-18" version="1">MSGFNRKRRFGNDDYQQPYQKRFRLPPIVELSKTLCGDINTLGESSKKYVEDVEFMTAPIIGNFENEKTLRDAVLNTLFAVVTEQPQKVLHISGLVQVVNAKKSSIGKTIAEFFHQKAQELIEQSINEEVIPESFETGPWNKIKLILRFLSTLSTFIKESSLVTLYSQFLKHSIDLQNNEPEKRCPLAEAIYYNTLISIPYLLSFGEPSEAMKEGIETILRIADDFKYVDEELTITKPFAVDSKAPYEPKNIISLILPAVKEFFKDDYKKVFSLFPQLSQSLKHLLTESEEFGLPQFSIPAVEAFKYITGLDTGLGSVDGMWRTPRLTFEVYLPILGFDTVPKSSDYAGLLFDDIVIDIVEALEFNRKEVARQIITLDLFFADGVFAPPATSIDQLKEINANNEELENKASTWKTEDVAIKNVLSLIFKLPTVSQPFAYFYTTLVEACTNAAQAIAPVLGRAIRFFYSNIHTADFELRFRYLDWLAVQLSNFNFTWKWREWETDSIKNSKSNYHPRIAFIKNLISKELRLATREKIQQTLTEEFHQYLDVKLFETEEIKEYYVSLFGEENLESAGLDFAEDGSKLFFLKENLSLKEDTEKLIEVFHKGSNKEYKTVVDGVREKVQNYPNPEKLVITLVFQIIAFVGNRSISHAGKYIGNTFEFLRSLLGRDEEESLERQLWAIDSILKYWNSAPENGYLVLDVLESYDVLSPKAMIKYSLDDSHKVNFGLVNVSATESIFRLLTNVAFSGKSSAEFEFIFNELMAIISSSLKTIENKGDIEVPSVEDDFDEKNEHIWKYQTSIGFLRAVLRKYSDEYVSLVPKLEESLKSIEHVPTKELVEKWFTELKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCC7</accession>
    <name>A0A1E3PCC7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_26791</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041829.1">
        <property type="nucleotide sequence ID" value="XM_019181301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCC7"/>
    <dbReference type="STRING" id="683960.A0A1E3PCC7"/>
    <dbReference type="GeneID" id="30198547"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019185">
        <property type="term" value="C:snRNA-activating protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050891">
        <property type="term" value="P:multicellular organismal-level water homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901002">
        <property type="term" value="P:positive regulation of response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902584">
        <property type="term" value="P:positive regulation of response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902806">
        <property type="term" value="P:regulation of cell cycle G1/S phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032875">
        <property type="term" value="P:regulation of DNA endoreduplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000037">
        <property type="term" value="P:regulation of stomatal complex patterning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033993">
        <property type="term" value="P:response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042796">
        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000355">
        <property type="entry name" value="Transcription factor MYB124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051575">
        <property type="entry name" value="Myb-like_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621:SF1">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="17"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="17"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="9">
        <location>
            <begin position="20"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="69"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="73"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="115" mass="13322" checksum="89864B54D1E56449" modified="2017-01-18" version="1" fragment="single">MSQQNNNNNKPPSSKSSTNLRRGPWSPDEDRKLIESINLFGATNWVRISQALGSRTPKQCRERYHQNLKPSLNRNPISPEEGQLIEELVAKYGKRWAEIARHLNGRSDNAIKNWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCC8</accession>
    <name>A0A1E3PCC8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Target of rapamycin complex 2 subunit BIT2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_66787</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041744.1">
        <property type="nucleotide sequence ID" value="XM_019184056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCC8"/>
    <dbReference type="STRING" id="683960.A0A1E3PCC8"/>
    <dbReference type="GeneID" id="30201302"/>
    <dbReference type="OrthoDB" id="2290221at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013745">
        <property type="entry name" value="Bit61/PRR5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32428">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT BIT61-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32428:SF2">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT BIT61-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08539">
        <property type="entry name" value="HbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="397" mass="44823" checksum="412384710BF65744" modified="2017-01-18" version="1">MSEDPFNRKRSTSTLANTISFTHTRGGGEEDRQQSNKPTSPFGYLNRNGSSSTIHSITHTLSNVGFNSVNPSIHSPESSTNSVTSPTQGKRENALTRAKLFARNKTKELKKTRDTDRPHHSHHHHNFLKINSHHGLSSSSSNSKVLDSGSTLYSFDPSNIIDNDVMKHVVSDANLKGLSFEEKDQIADNLWTTITSTVLPLFKLENSSSLKLKTPIEDVNKMVEVFLRLRIENKTTATSLISEVQELLKNGLNIMENELTFNENSDQGSPFHRLAITWEYFFANVYHYLLAIFLPLQLEFEGIGPIVKNAKEYWSDISEPSVTQSTKKVILSAFRDYVVIPYFDTELSVQDVEDSERRTLVQCFGILQSIHSANYNQRIIEHLSSILQQNLAPLKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PD29</accession>
    <name>A0A1E3PD29_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sidoreflexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_25033</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sideroflexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042069.1">
        <property type="nucleotide sequence ID" value="XM_019181089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PD29"/>
    <dbReference type="STRING" id="683960.A0A1E3PD29"/>
    <dbReference type="GeneID" id="30198335"/>
    <dbReference type="OrthoDB" id="6608471at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015194">
        <property type="term" value="F:L-serine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:monoatomic ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071454">
        <property type="term" value="P:cellular response to anoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140300">
        <property type="term" value="P:serine import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004686">
        <property type="entry name" value="Mtc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00798">
        <property type="entry name" value="mtc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153">
        <property type="entry name" value="SIDEROFLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153:SF6">
        <property type="entry name" value="SIDEROFLEXIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03820">
        <property type="entry name" value="SFXNs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ62862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="327" mass="34925" checksum="0708D5D7AA921DE6" modified="2017-01-18" version="1">MASSVPGPIALPESKFDLSTYWGRVRHCAEISDPTMLLTTSSSLDSAKKLISDYRNGILSKMTPELWHAKKVLDSTVHPDTGETVLLPFRMSCCVLSNLVVTAGMLTPGLGTAGTLFWQVANQSLNVAINTANANKSHPLSTTQLIQNYTIAVTASCSVALGLNSIVPRLKSLSQNTRLVLGRLVPFAAVVTAGIVNVFAMRSEEIRKGITVFDKNGDEVGTSKTAAKYAVGETAASRVINATPIMVIPPLILVRLQKGFLKGKGTGIQTLTNLGLILTTSLVALPFALAVFPQYQGISVHKLESTLAGKKDKDGNEIETVFFNRGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU57</accession>
    <name>A0A1E3NU57_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Multifunctional tryptophan biosynthesis protein</fullName>
            <ecNumber evidence="6">4.1.1.48</ecNumber>
            <ecNumber evidence="5">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_86194</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two components I and two components II.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48" evidence="6"/>
    <dbReference type="EC" id="4.1.3.27" evidence="5"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035928.1">
        <property type="nucleotide sequence ID" value="XM_019186009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU57"/>
    <dbReference type="STRING" id="683960.A0A1E3NU57"/>
    <dbReference type="GeneID" id="30203255"/>
    <dbReference type="OrthoDB" id="524799at2759"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005950">
        <property type="term" value="C:anthranilate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000136">
        <property type="entry name" value="Multifunctional tryptophan biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000031">
        <property type="entry name" value="Multifunctional tryptophan biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF4">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="15">
        <location>
            <begin position="6"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Indole-3-glycerol phosphate synthase" evidence="16">
        <location>
            <begin position="223"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ56721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="488" mass="53945" checksum="86C9BC9710207747" modified="2017-01-18" version="1">MSSKVVIIDNYDSFTWNLYEYLCQEGASVEVFRNDKISIQGVEELKPDTIVISPGPGHPLTDSGISRDVIKHFAGKIPIFGVCMGQQCIFSVFGGEVGYAGEIVHGKTSLINHDSKGMFQNVPQGIAVTRYHSLAGTKVSLPEDLEVTARTDSGIIMGVRHKKYTIEGVQFHPESILTEEGHRMIKNILNIRGGYWNDNENCTIFTDKEVKSSTTKKVKTSILDEIYAKRTLDVEELQKIPGFTFDDLKKNFDLGLAPPLVDFYDRLKNGGTESCLLAEVKRASPSKGPIDLQANSAEQGLLYAKAGASAISVLTEPHWFKGSIQDLKNVRQVVSSLPNRPAILRKEFIFSEYQILEARLAGADTVLLIVKMLNDALLKQLYNYSVELGMEPLVEVSSAEEFQRALILKPKVIGVNNRDLHSFQVDLNTTSSLVSLTPKDTLLIALSGITNGHDAEKYKAEGVNGYLVGEALMRAENVEEFVKDLLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUI1</accession>
    <name>A0A1E3NUI1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_86237</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ56786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035993.1">
        <property type="nucleotide sequence ID" value="XM_019186019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUI1"/>
    <dbReference type="GeneID" id="30203265"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ56786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="62" mass="6733" checksum="9DF024929FA70F9A" modified="2017-01-18" version="1">MQVKNQFLTKPGIVSDSMRNKVHRHMCRLVSRDQGLSATLGGVDFGGLGLQPTLFISAAHPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUK1</accession>
    <name>A0A1E3NUK1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bola-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_36924</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036013.1">
        <property type="nucleotide sequence ID" value="XM_019182490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUK1"/>
    <dbReference type="STRING" id="683960.A0A1E3NUK1"/>
    <dbReference type="GeneID" id="30199736"/>
    <dbReference type="OrthoDB" id="203381at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052275">
        <property type="entry name" value="Mt_Fe-S_assembly_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46188">
        <property type="entry name" value="BOLA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46188:SF1">
        <property type="entry name" value="BOLA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ56806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="113" mass="12948" checksum="DB914CD642BCD756" modified="2017-01-18" version="1">MFRFRSINSSFQGLRSINPTLKQNLLPNKRFYSDVRLSDYEQLIYNKLQKELSPQQLEVMDISGGCGSMFAISVVSENFKGLTMIKQHKLVNSILADEIAKWHGLQLKTKAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVH0</accession>
    <name>A0A1E3NVH0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Phosphomannomutase</fullName>
            <ecNumber evidence="5 13">5.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_36445</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>alpha-D-mannose 1-phosphate = D-mannose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11140"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="EC" id="5.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the eukaryotic PMM family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.8" evidence="5 13"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036406.1">
        <property type="nucleotide sequence ID" value="XM_019182438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVH0"/>
    <dbReference type="STRING" id="683960.A0A1E3NVH0"/>
    <dbReference type="GeneID" id="30199684"/>
    <dbReference type="OrthoDB" id="10264771at2759"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00424"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02585">
        <property type="entry name" value="HAD_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1240.20:FF:000001">
        <property type="entry name" value="Phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005002">
        <property type="entry name" value="PMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043169">
        <property type="entry name" value="PMM_cap"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466:SF0">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03332">
        <property type="entry name" value="PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00445">
        <property type="entry name" value="alpha-phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01143">
        <property type="entry name" value="C2.B.3:_Phosphomannomutase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01140">
        <property type="entry name" value="C2.B:_Phosphomannomutase_and_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ57199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="250" mass="28516" checksum="5E5268275C73AF3C" modified="2017-01-18" version="1">MSFQHKEKPDTLVLFDVDGTLTPARLTISPEFKQLLADLRKKVVIGFVGGSDLQKQVEQLGANVLNEFDYCFSENGLTAYKLGQQLASQSFIGWIGEEKYNELAKFILGYLANIDLPKRRGTFIEFRNGMINVSPIGRNASTAERNEFEQYDKEHKIREKFVQALKDKFPDVALTYSIGGQISFDVFPTGWDKTYALQHVEQEGFKEIHFFGDKSYKGGNDYEIYEDPRTIGHAVNSPDDTARILKELFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVX1</accession>
    <name>A0A1E3NVX1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alcohol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_36130</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036495.1">
        <property type="nucleotide sequence ID" value="XM_019182398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVX1"/>
    <dbReference type="STRING" id="683960.A0A1E3NVX1"/>
    <dbReference type="GeneID" id="30199644"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000947">
        <property type="term" value="P:amino acid catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08297">
        <property type="entry name" value="CAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000002">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="10">
        <location>
            <begin position="43"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ57288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="375" mass="39929" checksum="DB5A6F16EAA636FD" modified="2017-01-18" version="1">MLRTITNRLATQSRAPISRSFLRLNSSLAIPETQKGVIFYENGGKLEYKDLPVPTPKANEILINVKYSGVCHTDLHAWKGDWPLPVKLPLVGGHEGAGVVVAKGSSVKNFEIGDIAGIKWLNGSCMSCELCEQGYESNCIQADLSGYTHDGSFQQYATADAVQAAKIPKGTDLANIAPILCAGVTVYKALKTADLKPGQWVAISGAAGGLGSLATQYAKAMGLRVLGIDGGEEKEQLFKSLGGEVFIDFKKEKNIVEAIQNATNGGPHGVINVSVAEAAISQSTEYVRPTGTVVLVGLPAGAVCKSEVFSHVVKSISIKGSYVGNRADTREAIDFFTRGLVKSPIKIVGLSELPEVYELMEQGKILGRYVVDTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW21</accession>
    <name>A0A1E3NW21_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_46402</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036578.1">
        <property type="nucleotide sequence ID" value="XM_019182595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW21"/>
    <dbReference type="STRING" id="683960.A0A1E3NW21"/>
    <dbReference type="GeneID" id="30199841"/>
    <dbReference type="OrthoDB" id="648285at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000119">
        <property type="entry name" value="MFS monosaccharide transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF73">
        <property type="entry name" value="METABOLITE TRANSPORT PROTEIN YDL199C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="272"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="337"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="431"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="26"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="538" mass="59682" checksum="BAC29CEA4C532D6B" modified="2017-01-18" version="1" fragment="single">MTDHSEYQQLTLKESYLVGKPLLYFTTCFVSTGVLLFGFEQGVMSGLLTNHRFKDYFKDPSSAAIGTMVAILEIGALISSLLSGKVSDKVGRRRAIRYGAIIFAIGGALQALAPSLTILTIARFISGLGVGLLTTVVPMYQTEISPPHSRGRLGCVEFTGNIMGYAISVWADYFCSFLESNAAWRIPLSIQSLIAIALYFGSYVIVESPRWLLNHDHDAEGIVVIADLTSGGNVQDERARNEYREIKETVLLHRLEGESSYRYMFKRYKKRVFIAMSGQAFAQLNGINVISYYAPLVFEQAGWEGRDAILMTGFNAIIYVLSTLPPWILVDKWGRRPILLSGAIVMGVALTAVSYSLYVNTEKTRHLVVILVFIFNAGFGYSWGPLPWLIPSEISPLSIRAKIASLATASNWAFNWLVGEMTPILQELITWRLYLIHSTSCFISFFVVYTVFPETAGVSLEDMDSIFDDRSSTFSFHGSTYQPIDSEAQSIADETVPGGNIHQNLSTAALARQQALANPTVDYSNPLLTKQKSEQSFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW28</accession>
    <name>A0A1E3NW28_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_71266</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036543.1">
        <property type="nucleotide sequence ID" value="XM_019184237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW28"/>
    <dbReference type="GeneID" id="30201483"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="145" mass="17070" checksum="D3ED8594DA386714" modified="2017-01-18" version="1" fragment="single">MSARRDTSDAGAADLDDNIEDEIDKILDMYEYIMEKFQDFLKKELKSSKIMKKQHQDQEISCINPGSRISRNNRDNIPLSVENNKKLMKKREKSVEMKKLQSLDGEKIITDEKEIQDEALLFYNNLFNIKSELADDELERKSEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW82</accession>
    <name>A0A1E3NW82_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_64738</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036591.1">
        <property type="nucleotide sequence ID" value="XM_019183794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW82"/>
    <dbReference type="GeneID" id="30201040"/>
    <dbReference type="OrthoDB" id="10572718at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="119" mass="13895" checksum="88053CD68B469816" modified="2017-01-18" version="1">MELFIGQFDQFIDHLVWKATKTTLIILNIDQELSIPSNFQRSSVQYLKNVELIDLIHIDSLQKLMVYLSMKQVNHNDESIAIWELPSENLILSKLTKLNNKVILGFNTTTDLSTYKKWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWD2</accession>
    <name>A0A1E3NWD2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HCP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_35745</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036688.1">
        <property type="nucleotide sequence ID" value="XM_019182348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWD2"/>
    <dbReference type="STRING" id="683960.A0A1E3NWD2"/>
    <dbReference type="GeneID" id="30199594"/>
    <dbReference type="OrthoDB" id="272077at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051726">
        <property type="entry name" value="Chitin_Synth_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430:SF3">
        <property type="entry name" value="ACTIVATOR OF C KINASE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430">
        <property type="entry name" value="PROTEIN SKT5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="442" mass="50032" checksum="96AC76B4E5C4ECED" modified="2017-01-18" version="1">MTKSPSRVLDPNLINKSTEQTTKRKPPPTAEPQQPHEEVPIALQPNKSLYSDEVSLSNFNRIKDFAIEDFRKGNFSADPQIDWIKTMLQYACDEQFMKECNINGEKLTRTLSPNDVKKNEQIFIKQAIKLLKKVVHVTKDPDAQYIVACLYSNSPSIKVPYPAILEKDYAKSFDYYTKAANQGHPLAMYRLGVSYEIGIGTSQNDKKAMECFIRSAEFGCVASMFKLGMIYSRGALKVSRSASKSLNWLNKAAELASKENPHSLFELAKYHEYDFPFLKNETLTQSDANFLKELQSLGLNKDERVALQYYKEAAKLLYAPAQCKLGWCYEYGKLGCVIDPRRSIGWYSRAAKQGNFIAEMALSGWYLTGASGVLEPNDKEAFLWARKSAESGFVKAEYALGYYYEVGLGCQADLELSRKFYLKAATQGHDKAIAKLREMRGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWQ3</accession>
    <name>A0A1E3NWQ3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_64786</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S14 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036647.1">
        <property type="nucleotide sequence ID" value="XM_019183803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWQ3"/>
    <dbReference type="STRING" id="683960.A0A1E3NWQ3"/>
    <dbReference type="GeneID" id="30201049"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006746">
        <property type="entry name" value="26S_Psome_Rpn12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033464">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12387">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12387:SF0">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10075">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="78"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="271" mass="31815" checksum="C03E7AA1A91BF7A5" modified="2017-01-18" version="1">MPSLEDLTKQLKSAFEAKQYSKTEELLSPIKILLIQNDLLIPNYQKINKDPHYVEDLVISRSILEIGALSSINSQDFDRFENYLQQLKVFYFNTVSNEQLSKSVNKSKLISLYLLLLLSKGDVVKFHTELEFLGNHLKNIENDLYLSYPIKIENWLLEGYYDKAWELISSKNSEEKIKLKEFNIFNDTLLQAIRFEISRSIEKTYKKLPFSNAKYLLFLNSEKEVEAFAKEQNWEYSNGELLFNLSAKEDKFGFTQDEEDSQLNNSEKLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWQ8</accession>
    <name>A0A1E3NWQ8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_64903</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036760.1">
        <property type="nucleotide sequence ID" value="XM_019183829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWQ8"/>
    <dbReference type="STRING" id="683960.A0A1E3NWQ8"/>
    <dbReference type="GeneID" id="30201075"/>
    <dbReference type="OrthoDB" id="409928at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000006">
        <property type="entry name" value="40S ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17509" checksum="F1FB595EF6E1898D" modified="2017-01-18" version="1">MSLVNLGNIAAHLQNVTRVHLATTSIPFSRLHLEVAAHLYKQGFISSVQRGSLQGPDKVPTEVTPDNIATRRLWLGLKYRGTDSVIRKFSLVSKPSCRIHLNKDELKLLAAGRKVRLIKPLQPSELMLIKDNKTKKVLDLAEAVNKGVEGEVLCRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWU6</accession>
    <name>A0A1E3NWU6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_80987</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036866.1">
        <property type="nucleotide sequence ID" value="XM_019185159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWU6"/>
    <dbReference type="GeneID" id="30202405"/>
    <dbReference type="OrthoDB" id="3979586at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032218">
        <property type="term" value="P:riboflavin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF177">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER MCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="82"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="330"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="385"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="449"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="5">
        <location>
            <begin position="84"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="29"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ57659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="482" mass="52580" checksum="E1A179AED51AB66B" modified="2017-01-18" version="1">MITIDRFERPSILIPDPAKHRHDSIELNDQTPAEEGTSTAIGITKQQSHTQRTQEIYDGGDDDPQIELVDEDISFPDGGAEAYLVVFGALLSLIITFGLMNTIGAIQAYLTTHQLANASASSVGWIFSVYFFFAFGGGIYAGPIFDYKGARLPMFIGSVLIIIGLFLTANCSKVYQFVLAYGVIVGVGSSFLMSASLGAVAHWFNKKRATALGICSVGGSLGGIIWPLMLRSLYPKIGYAWSMRILAFCSILCLGAGCLLVKHRLTRPTSDEAKSPKQILKDSFVLKDLVHEPRFLALTISVFLCEFSLILVVTYMSSYTIYQGYSESDAFLVLIVCNTTGVLGRYLPNHMGDYWGRINIMLVTVFVCAILILVVWLPFGHSLSSMYAFAGLYGFFSSSTLSLTPVCCGQISRTEDFGKRYGTVYFLVSFGNLIALPIGGAIIGDGSGYNNLIITAGVFEAVAAGFWLLTRYYCVGMRLCKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX51</accession>
    <name>A0A1E3NX51_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">T6SS Phospholipase effector Tle1-like catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_34864</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036935.1">
        <property type="nucleotide sequence ID" value="XM_019182246.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX51"/>
    <dbReference type="STRING" id="683960.A0A1E3NX51"/>
    <dbReference type="GeneID" id="30199492"/>
    <dbReference type="OrthoDB" id="3162439at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018712">
        <property type="entry name" value="Tle1-like_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33840:SF2">
        <property type="entry name" value="TLE1 PHOSPHOLIPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09994">
        <property type="entry name" value="T6SS_Tle1-like_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T6SS Phospholipase effector Tle1-like catalytic" evidence="3">
        <location>
            <begin position="39"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="311"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="276"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="655" mass="75167" checksum="60177E1DE5E3E118" modified="2017-01-18" version="1">MSVKELLNHKLKSVIVDEEDVSQSASSDGIRTGDLKKGKNIVLCFDGTHNKFGPSPYTNLLKLFRMLEKDDPESQICYYQPGIGASMSVESESMFERYLSKQSLSDNLDSLIAFSLDQHIIDAYKYLMRFYQNGDKIYLFGFSRGAFIARVLSSMIDRVGLLNAGLEEMAESAWSIYSAWEYAAQPSQPDYTTTLIDEYKKTFSRVDTPRIEFVGMFDCVNSVGLLRDRLFPLSTKSGLIKNLSHAVSLDERRGKYRQNLIFPFSYKPSFFSLTSTEQSSGNSSRKVTNAGSSTKTSVSSSISSILLEHFGEKSQKKLNDAQKLMKDIEQKLKETSRMSIRSRLKRVETNNNNTNGTDHQASFSTFGSSSDKQKLITGEYKEVWFAGNHGDIGGGWASDVNGHFLSHIPLRWMFGEALRVGVIFQRERLVNFANRFSSLDSMLSCNHDMLSFYKGELTHEISQAERETVLELSKATFNKAYIPDFRSCEVISDQASFEQFSPIQGYDGRGNSSIWNVLGWWIVEFLPIGTKIEDEDDKWRNVYVPNLGRPRSVPQYSKLHWSVYWRMKLCYDYLPRNLPEYVENILNERRSNHNDSESRDELMEFDVSSAFDDHTSDLIKKLIMETRLLFKQWDSEHWKQVPDDLNPIMRELGYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXG1</accession>
    <name>A0A1E3NXG1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">6-phosphofructo-2-kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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    </organism>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
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            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
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        <property type="term" value="F:ATP binding"/>
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        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="6Pfruct_kin"/>
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        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="6PFRUCTKNASE"/>
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    </dbReference>
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    </dbReference>
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            <end position="106"/>
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        </source>
    </evidence>
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</entry>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
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                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="27535936"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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    </evidence>
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    <sequence length="267" mass="28631" checksum="B507237819B1F760" modified="2017-01-18" version="1">MSGIRSNNPFAQEAEEEIQSHISNSNGLSRQSSVNNNRTPDPSILESSSTEAITLDGHGGSTQQNNTNGSTNNSEPIIPSIQVNQDTEPAQVDGGSLHGNSRRSSTSTNNNYSIPEWQQVDDSHPDGPPPAYSEVDPISPSAANQSSNLYQRPTEEPPRSPSTGQYERPSAPFPPQRPQIDTQFAPPPGPPPNSHFPPPGPPPNSFMPPPGPPPSHLSPEEFSPRLPPRPTSPRLPPRPNVPRRNIYPGASGASYSNIGAPPLPNRR</sequence>
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<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXV0</accession>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
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                <person name="Calvey C.H."/>
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                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
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                <person name="LaButti K.M."/>
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        <source>
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        <text evidence="1">Belongs to the ATG14 family.</text>
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    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00009574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="244" mass="28980" checksum="42FC40F923E3A7DA" modified="2017-01-18" version="1">MVSYNDEIKSIQSQTKTTKENSLNIEKQINNSQSILFQELINLYLVKRKRLSGVKNQYIFMISFIPIINLENLLSFNFEIINASLERICKFIYQISTIWFINLPFQIEFNHQQQPSILNFKLFSPDSNIEQIHDLSNFELKLFLNGISRLILNIFEILKFFQLDNEIKLSKQLFNIDEMIYKIVNNNYNFNELSSDNDQSNPIKGNIDIDELTDLVYKHILNKINQKNNEWHVVQNDFLIDEDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY31</accession>
    <name>A0A1E3NY31_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Tubulin-specific chaperone A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_35042</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Tubulin-folding protein; involved in the early step of the tubulin folding pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
            <location evidence="2 9">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the TBCA family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036694.1">
        <property type="nucleotide sequence ID" value="XM_019182265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY31"/>
    <dbReference type="STRING" id="683960.A0A1E3NY31"/>
    <dbReference type="GeneID" id="30199511"/>
    <dbReference type="OrthoDB" id="296187at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048487">
        <property type="term" value="F:beta-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.90:FF:000010">
        <property type="entry name" value="Tubulin-specific chaperone A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004226">
        <property type="entry name" value="TBCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036126">
        <property type="entry name" value="TBCA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21500">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21500:SF0">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02970">
        <property type="entry name" value="TBCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46988">
        <property type="entry name" value="Tubulin chaperone cofactor A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="103" mass="11711" checksum="1E3BEB481520F9C2" modified="2017-01-18" version="1">MAPTQLEIKSKSLARLIKEEGLYQKELKEQEEHVQGLKSSNADSYEIKKQEEVLEDTRKVIPEVRKKISEAQESLESYITDYTGTEDLTAAKENIEAAKKLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY43</accession>
    <name>A0A1E3NY43_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_64072</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037127.1">
        <property type="nucleotide sequence ID" value="XM_019183668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY43"/>
    <dbReference type="GeneID" id="30200914"/>
    <dbReference type="OrthoDB" id="3980803at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="157" mass="18096" checksum="C4BC09926995F2B0" modified="2017-01-18" version="1">MANPLLHQHEDTAQGSPCLKKRKLEEEEEVEGGAEVSKLKQFQSKPIEENIDYISLKSSLSLLQSNLKNLERNIHELSSFKKTIKESNNIKEIAGLINENSNYLTEITYKGSCIKCPVINWNLNYGLKLEKLNDDDECYEKINEGYEILKRDKLFNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYC8</accession>
    <name>A0A1E3NYC8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Flavin-containing monooxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_85234</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FMO family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037320.1">
        <property type="nucleotide sequence ID" value="XM_019185864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYC8"/>
    <dbReference type="STRING" id="683960.A0A1E3NYC8"/>
    <dbReference type="GeneID" id="30203110"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="56816" checksum="C57D6DF2A83DF87B" modified="2017-01-18" version="1">MTHTNEVRVLPRKVKSVAIIGGGASGAIALDSLIKEEKFEKIVLFERRDVLGGVWVLDHKPDQLDVPPGFTQDQLDPKVKIPKDFQNAKDVTKLPRTQQQRYLHTASYEDLRTNIPEQLMTYSDEKTWGADEKLYVDGHYIRGVAIRDYIDRYINRHRDHVVLRTTVEDITKDYSTSDTQFELTLRTETDEKDNDGNYIDHWRKESFDSVVIATGHYHVPSIPKVPGLDEVYHRFPSKVKHSKTFRANDDFKDQTIIVIGSRASGADIVDITSKDAKFVYQSRRSKGPLRFDGAENTTLKPVITKYELVNDQDIIVHFADGTTVENPDQIIYATGFRFSYPFLKDLYPNFTTGYINPDLYLHTFSIKDPLLSLIGVPTDAISFRAFEYQAVLVSRFLAGKVVLPPLNEQLQWTLQRFIEKGDQRAYHTIDFGKKHEFLNLLTSIGGGVEPIGQTGRPFPTWTEDDLQLLLSIEERFKKFFGEGEANDFKNSTIKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYM4</accession>
    <name>A0A1E3NYM4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_85307</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037430.1">
        <property type="nucleotide sequence ID" value="XM_019185875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYM4"/>
    <dbReference type="STRING" id="683960.A0A1E3NYM4"/>
    <dbReference type="GeneID" id="30203121"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006598">
        <property type="term" value="P:polyamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000050">
        <property type="entry name" value="Aldehyde dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000012">
        <property type="entry name" value="Betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43720">
        <property type="entry name" value="2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43720:SF2">
        <property type="entry name" value="2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="6">
        <location>
            <begin position="28"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="501" mass="55079" checksum="0BBA4935F828FC8D" modified="2017-01-18" version="1">MSDLFVDIQLPTGESYKQPIGLFINNEFVEGHSDELISTINPTNGELITKVHSANEQDIDIAVKSARETYENVWSKTSGSAKGELLYKLAQLIEEEKEILAKIDTLDAGKPYHSNSLGDLEQIIELTKFFAGYADKINGKYIPISDDKFVYEIKEPYGVVGQIVPWNYPLAMASWKIQSAIAAGNTVVIKSAENTPLSLLYFGQLVKKAGFPPGVINIVSGLGPIAGSAIAKHEDIDKVAFTGSTNVGKIIQKLATSNLKAVTLECGGKSPSVVFNDANLKEAVKWSSLGIYYNTGQNCTANSRILVEEDVYDEFLVKFKQYVLENWITGDPFDSKTTVGPLISKVQLDRVLEYVKIGEDEEHLEKLELGDKAIYSKHNGWFLNPTVFLDVPVDSRLFQEEIFGPVVTITKFKGYKDALSKANNTQYGLGSAVFTKDIKKAHKFGKGLKAGTVWINSSNDEDIRASFGGYKMSGIGRELGESGVDVYTQIKAIHVNLADDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ01</accession>
    <name>A0A1E3NZ01_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_33989</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037515.1">
        <property type="nucleotide sequence ID" value="XM_019182139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ01"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ01"/>
    <dbReference type="GeneID" id="30199385"/>
    <dbReference type="OrthoDB" id="10020333at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031431">
        <property type="term" value="C:Dbf4-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902977">
        <property type="term" value="P:mitotic DNA replication preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001100">
        <property type="term" value="P:negative regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045739">
        <property type="term" value="P:positive regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903468">
        <property type="term" value="P:positive regulation of DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060903">
        <property type="term" value="P:positive regulation of meiosis I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905263">
        <property type="term" value="P:positive regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905342">
        <property type="term" value="P:positive regulation of protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904968">
        <property type="term" value="P:positive regulation of spindle attachment to meiosis I kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031503">
        <property type="term" value="P:protein-containing complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14019">
        <property type="entry name" value="STKc_Cdc7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF23">
        <property type="entry name" value="CDC7 KINASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="87"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="484" mass="55271" checksum="7BA90771E0A47038" modified="2017-01-18" version="1">MKSNKPIASSNTSHTNNPTALKHEHATIPKSRDIKKEQPLQQQPPSKTNQIRKSETREASDDSDIPLEILEEMDKVQETFQDLDKKYRLIDKIGEGTFSTVYKAEDITGKTRYLMGDSIWSSPDIKRRKTKKKSTKVYVALKRIYVTSSPQRIFNELNLLYMLSGSSRVAPLLEALRYQDQIIAVLPYYAHADFREFFRDLPLAGIKIYMYELFEALNFVHDKNIIHRDIKPTNFLYDPFKKRGVLVDFGLAEQEFPPNSTDMNAVGYCPCVSHERFSTEETLKGYPKDDPRPGRRANRAGTRGFRAPEVLFKCPNQTRKIDIWSAGVILLTLLSRRFPFFNSPDDIDALVELTTIFGLKKMQECANLHGLGLDSTLNTLNKTTTLDELIHWCLALEDNGDTLPKDCVAYDTLSVFNTDGHIVFGNSDDDKTISLKRQHQNAIEVMNHCFELDPSKRSSAAQILRLPFFSSMNGEEDDEVLIID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZT6</accession>
    <name>A0A1E3NZT6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_79267</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cclA family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037928.1">
        <property type="nucleotide sequence ID" value="XM_019184867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZT6"/>
    <dbReference type="STRING" id="683960.A0A1E3NZT6"/>
    <dbReference type="GeneID" id="30202113"/>
    <dbReference type="OrthoDB" id="10266026at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042800">
        <property type="term" value="F:histone H3K4 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12872">
        <property type="entry name" value="SPRY_Ash2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037353">
        <property type="entry name" value="ASH2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10598">
        <property type="entry name" value="SET1/ASH2 HISTONE METHYLTRANSFERASE COMPLEX SUBUNIT ASH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10598:SF0">
        <property type="entry name" value="SET1_ASH2 HISTONE METHYLTRANSFERASE COMPLEX SUBUNIT ASH2"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="105"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
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            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ58721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="410" mass="46856" checksum="D3525C2851BA27A3" modified="2017-01-18" version="1">MSSTEPVIPQKRNLESEERHQYQTRSTTTTSIPNNQQLDSHDIKALYSKKKNIVINKFKPIISYKPIDFEQFNAPLAKSSFDPNFHIIEDIPFNTRRGFQYSPCRPNPDLSLLKFSTSDLPPFNASLSYFDRSSGTFISGDYNSVTTDKGWLSARTNIPIQEGKVYFEFNIVNSNEQSHVRIGVGRREASIEAPIGFDAYGYGLRDKTGQKLHLSRPVKFMKEGFKSGDVIGFLVELPKTEFQDIVRDQIAIRYKNRLYFERFDYTPTEKMEHLLNPVTVFGEKAIPDLNPFKPDFLPNSSIKIYKNGEFIGTAFEDLYAFLPPYSEQKNNINKLNNNGTLGYYPTVSVFKGGIAEINPGPNFKCKPNDLEPDVKEFHELYNEKIADDVVWDIIDEIEAEAIQTTIDNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZU2</accession>
    <name>A0A1E3NZU2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_70153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ58202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019184194.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="30201440"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="189" mass="21368" checksum="7B9358321E92C4C5" modified="2017-01-18" version="1">MPSVPTSTTRFFRTPGPFIVNSRDRTFQRYTEAELEIGPIASTPSANIDIVFGGPLVGFIDVTGFDDGYDPDISEGETIESIKTPLVPVEGDNVKDGTLYAVNLDNYYKEYCAYSGDEDTDEEDEEVCTEVRMLLKEPIDEASQRQMLEHRATSSEEENLVRNSSDDRFDMEEERRTTSKSSEMSRFVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P064</accession>
    <name>A0A1E3P064_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Trehalose-6-phosphate synthase</fullName>
            <ecNumber evidence="6">2.4.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">UDP-glucose-glucosephosphate glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_31819</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>D-glucose 6-phosphate + UDP-alpha-D-glucose = alpha,alpha-trehalose 6-phosphate + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58429"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="2.4.1.15"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.15" evidence="6"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038036.1">
        <property type="nucleotide sequence ID" value="XM_019181885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P064"/>
    <dbReference type="STRING" id="683960.A0A1E3P064"/>
    <dbReference type="GeneID" id="30199131"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005946">
        <property type="term" value="C:alpha,alpha-trehalose-phosphate synthase complex (UDP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071465">
        <property type="term" value="P:cellular response to desiccation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000007">
        <property type="entry name" value="Trehalose-6-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000035">
        <property type="entry name" value="Trehalose-6-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012766">
        <property type="entry name" value="Trehalose_OtsA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02400">
        <property type="entry name" value="trehalose_OtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF106">
        <property type="entry name" value="BCDNA.GH08860"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="475" mass="53869" checksum="6F0E23A7E7068496" modified="2017-01-18" version="1">MSPPGNVLVVSNRIPVTLKKTEDGQYQYNMSSGGLVTALQGLKKTSTFQWFGWPGWEVPEKDQAKVTKDLDEKFNASPVFLNDDVAELHYNGFSNSILWPLFHYHPGEINFDENAWAAYNEANRAFATEIMKHVKDGDVVWVHDYHLMLLPELLRDEIQKRGIKDVKLGFFLHTPFPSSEIYRILPVRQEILKGVLSCDLIGFHTYDYARHFLSSVERILGVKTLPNGVEYKGRYVNVGAFPIGIDVGNFTEGLKKPEVIKRVTALKEKFKDTKIIVGVDRLDYIKGVPQKLHAFEVFLNEHPEWIGKVVLVQLAVPSRGDVEEYQSLRATVNELVGRINGTFGTVEFVPIHFMHKSIPFDELISLYSVSDVCLVSSTRDGMNLVSYEYIACQEELKGSLILSEFTGAAQSLNGALIVNPWNTDELAQAIHESLTLPDEKRAANHTKLFNYISKYTSAFWGENFVKELYKIDNQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0Q1</accession>
    <name>A0A1E3P0Q1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kish</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_31998</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Involved in the early part of the secretory pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Golgi apparatus membrane</location>
            <topology evidence="2 9">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the KISH family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038172.1">
        <property type="nucleotide sequence ID" value="XM_019181907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0Q1"/>
    <dbReference type="STRING" id="683960.A0A1E3P0Q1"/>
    <dbReference type="GeneID" id="30199153"/>
    <dbReference type="OrthoDB" id="10034655at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051523">
        <property type="entry name" value="KISH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009653">
        <property type="entry name" value="Ksh1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13229">
        <property type="entry name" value="PROTEIN KISH-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06842">
        <property type="entry name" value="DUF1242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009027333" description="Protein kish" evidence="9">
        <location>
            <begin position="26"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU910717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="70" mass="7920" checksum="27705176DE1A18AA" modified="2017-01-18" version="1" fragment="single">SALFNFSALLQVILLLICSCTYIHTNWPSLLDRNHHGLLGVFWKCARVGERASPYVSIALIYMAINQIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0R1</accession>
    <name>A0A1E3P0R1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PIG-P domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_15671</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038001.1">
        <property type="nucleotide sequence ID" value="XM_019180653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0R1"/>
    <dbReference type="STRING" id="683960.A0A1E3P0R1"/>
    <dbReference type="GeneID" id="30197899"/>
    <dbReference type="OrthoDB" id="690928at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052263">
        <property type="entry name" value="GPI_Anchor_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013717">
        <property type="entry name" value="PIG-P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08510">
        <property type="entry name" value="PIG-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="PIG-P" evidence="6">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="114" mass="13558" checksum="57641390A5C5AA10" modified="2017-01-18" version="1" fragment="single">EYRGFALYLIISVFHLIWLCWTFAPARYLEKIGIYYYPSRWWSLSLSCTVLMGMLYTYIALQMWNVEVETLKLDDLNTIVDDDAVLEKDLDKYGWCDTNGVYDLRITDVNKVLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0R7</accession>
    <name>A0A1E3P0R7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_69456</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038276.1">
        <property type="nucleotide sequence ID" value="XM_019184166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0R7"/>
    <dbReference type="STRING" id="683960.A0A1E3P0R7"/>
    <dbReference type="GeneID" id="30201412"/>
    <dbReference type="OrthoDB" id="298012at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF178">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE YGL185C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="4">
        <location>
            <begin position="65"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="185"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="365" mass="41136" checksum="B3EA79FBD2838BF7" modified="2017-01-18" version="1">MSKPQVLFIGDLNESLDEFQAFKQKFEIIHYKLTTKQDFIHSLTTRFQSISAIYCGWAGFMPIGGLTDDLIQLLPQTLKVITCCSVGYDPYDVESLKTKGIYFYNTPSLGADHVADLVLWHVLESFRKFSTFQRITNDKGDTVKTRGSLQTHGYDTEKGGLGVDQDEWERETYPFGHICGGLKVKGPRGSKIGLIGFGKIGQKIGKRCDALGMNVYYHKRQPLDKLEELILGYKVEYLELDELLQNCEVIVICCPGNKSTMNLLNRERLDLIQSNGKIINVGRGFIIDEEYMISKLEKGELGFIGMDVFTGEPNINPRLLNRSDVSLTPHIGSSTEDVFDNTAIFNLKNIYDELIMGKDGESRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0T1</accession>
    <name>A0A1E3P0T1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Early meiotic induction protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_32355</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038140.1">
        <property type="nucleotide sequence ID" value="XM_019181946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0T1"/>
    <dbReference type="STRING" id="683960.A0A1E3P0T1"/>
    <dbReference type="GeneID" id="30199192"/>
    <dbReference type="OrthoDB" id="2017405at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021475">
        <property type="entry name" value="Pants/Emi1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28052">
        <property type="entry name" value="UPF0545 PROTEIN C22ORF39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28052:SF1">
        <property type="entry name" value="UPF0545 PROTEIN C22ORF39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11326">
        <property type="entry name" value="PANTS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="78" mass="9125" checksum="4F3C360E771124E9" modified="2017-01-18" version="1" fragment="single">TTMSCTEAFDQLVACYSLGGQIRHYYRYGEMNDCVSQFSKFKFCLTNSDPVKIQQFYKETLDKKKDTGSSEDVWELRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0T9</accession>
    <name>A0A1E3P0T9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aspartate/glutamate racemase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_96599</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037744.1">
        <property type="nucleotide sequence ID" value="XM_019186522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0T9"/>
    <dbReference type="GeneID" id="30203768"/>
    <dbReference type="OrthoDB" id="412093at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="264" mass="29262" checksum="6E5D599D4C8231FF" modified="2017-01-18" version="1">MSTYPPRDQLPPLGALGIDYTEDIHRPPGDPLNELSYHFPVIHQTVPNATVPNVVASGKFSEEFLQAFVDACNSLEKRGAIGIITSCGFLAQVQHRLAEKINIPIATSSLLQVPLVLSILSPKKKVAILTFDGETLGEAHFEGIGVTPEMRKRVKVFGCKDGGHLRNLCIEGTPYVHEELEKELVELAETAVSEDDSFGAFVLECTQMPPTSKAIQKATGLPVYDAITMIDWFYSGLRTRIIPEDKFKEDGLRPRKREPKDKYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0U2</accession>
    <name>A0A1E3P0U2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Oligomycin resistance ATP-dependent permease YOR1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_44143</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038222.1">
        <property type="nucleotide sequence ID" value="XM_019182581.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P0U2"/>
    <dbReference type="GeneID" id="30199827"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18597">
        <property type="entry name" value="ABC_6TM_YOR1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18606">
        <property type="entry name" value="ABC_6TM_YOR1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001750">
        <property type="entry name" value="ATP-binding cassette transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000010">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="269"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="299"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="380"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="768"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="808"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="887"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="157"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="472"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="775"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="1087"/>
            <end position="1330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ59015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1338" mass="150518" checksum="60A1B547768C76D7" modified="2017-01-18" version="1">MPLFDRFSKRSTVDVNLKEQSTITAESDSAQAELEAPVLLKKTSPLITFIFGKKVPPIPEKREPYPFVNANIFSQLTFSWISDMIKRGYYRRVEDEDLYYLVGDLTIEEMCKKFEANMDRRILEWKKKKQSQPEERFTKYLVLRALNDTFFKTYWSAGAFRVLCDTSQVLNPLLVRELVKHVNAQQTDPTLGPGKAIGYCIGISLLLIVSSLGNCNCLHLGMLSGAQAKALLTNLCYKKSFKLSAKGNSEFPKGKVNSLVMTDLARIDMALGVIHFLWSFIIALIIAIVILLVLVGAPALVGIATCFVIIGYIFVANKYLKSLRRKTIAFVDNRVGGVREVVNAMKMIKLYCWEVPYFKIIEKYRNDEKKHLLKMNLFKASMFSLMTSTGVLATMFTFLTMYGVGKNFKSYNIFSSVALFNSLRQPLTVLPSATAYVIDGSIALDRITAFLQADEKEEYVEHHEIQESENSIEVEKATFGWDLDGEVAKQEILKDISLSIKRGEFVVITGAVGTGKSSLLSAIHGMMIKYLGSVKVYGSQSFCSAPWIQNETIRENILFGNTFDHKKYSRVIKACSLEDDFAMFTHGDFTEVGERGITLSGGQKARVSLARAVYTNSDIILLDDVLSAVDARVGKHIMDDLICGYIKDKTRVLATHQLSLIQSADRIIFLDGSGGIDVGTEDELLSRNNDFINLMKFSSEKEGAEEELEDPQEVGDTIKKISTIISAKEDVGGDEQKKHKIIEEEFRSAKSISWEVYYNYFSLGAGRFYFIVLPIFGLSCILNGFLFAFNSVWLSFWVSDKFGFSSHVYAGLYAMFCVLAALANVSMLLLLGKLNNDAGLRLFILALQKVLRTPMSFMDTTPIGRILNRFTKDTDTLDSNIADQLSIFAMGSISVCSTFILCGIYIPYLFIAYPFIGFFYISLSNYYQTSALDIKRLEAINRSNVFSHFNETLSGMSTIKSYGSVKRFSERFEYLIDKMDMAYFPTLANQRWLAVRLDFTASGVSLLVSLLCVCGVFNLGSSSTGLLISYVMQIASIVSMLMRSKTQIENDMNSCERLYEYAYQLPQEAAPLITPGPKPTWPEQGSIEFENASLRYRDNLPLVLKNVTFNVAPGEKIGIVGRTGAGKSTIMNALFRINELATGKVQIDGIDISTLGLDQLRSKMSIIPQDPVLFKGSVRQNLDPFNSYNDVELWDALKRSWLIEKDSELTLDANETMHKFHLDQHIKDEGENFSLGERQLIALARALVRNTKILVMDEATSSVDYETDSKIQSTIANEFSQCTILCIAHRLRTILKYDRILVLDHGEVKEFDTPFNLFKQNGIFTDMCQRSNIIEADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0W2</accession>
    <name>A0A1E3P0W2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcriptional regulatory protein SDS3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_63583</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038288.1">
        <property type="nucleotide sequence ID" value="XM_019183575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0W2"/>
    <dbReference type="STRING" id="683960.A0A1E3P0W2"/>
    <dbReference type="GeneID" id="30200821"/>
    <dbReference type="OrthoDB" id="70376at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013907">
        <property type="entry name" value="Sds3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21964">
        <property type="entry name" value="BREAST CANCER METASTASIS-SUPPRESSOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08598">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01401">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="171"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="31123" checksum="BCD1550C2CA220A0" modified="2017-01-18" version="1">MSKKDKKRATVQGKLNKMDENFKLDKDYHYRSMLQDLQRSLSTMHQGTNEVLVERMKDFEESRDYELVELRLWEEYQVQRAEKEFFEEVEKANTEHDEMVKLVKEKLYANLEKQIKQLKEDKILLDLANSHSYNMDTSLTNVHDFHKNTRSSNSLKDSTISSIGYMSDRRGLRRRDMNYSTNNDDSHLSANDSGNNSSSKKRKTNGSSGRYSSNDETFMSDNGDLSSILFGEKPEKVHTRHSSKSFVTPPGLKPEEINDDLLLMKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0Y5</accession>
    <name>A0A1E3P0Y5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MOSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_63636</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038346.1">
        <property type="nucleotide sequence ID" value="XM_019183586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0Y5"/>
    <dbReference type="STRING" id="683960.A0A1E3P0Y5"/>
    <dbReference type="GeneID" id="30200832"/>
    <dbReference type="OrthoDB" id="17255at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005303">
        <property type="entry name" value="MOCOS_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03476">
        <property type="entry name" value="MOSC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133625" description="MOSC domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="MOSC" evidence="2">
        <location>
            <begin position="205"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="375" mass="43592" checksum="B98A8676BABFF92E" modified="2017-01-18" version="1">MILVSLLFLSSLVSFIFLPQLIKSLNLSTTTPDNELSNILRWLLNFRYYLISKLLFFKYPAKVGKIDKIFVYPLKSVAYIQPTKWEIDEHGLKHDRQYMIGFWDSKGNCYQGYTLRNAPRLALVSIDYNLEENWFKFTYPKLDGSTSFFKLPCNVSKTFLETHAIGEDKEKITDLWGIKFDSINLGSDLIPQDFYDSMGLNREGTTLLYSSKGKFVKTAHPKDLEQMRKVLFHDYYPIHLISQRNVDELNQRIKDSGTKDRYVEALQFRPNVVIGGNDKIELDSWYKVLINGHLWSVVQKTPRCSIGNVRMDKGDFDKSNSVTRTLRSYRRIDPGDKNGIFLGDYAIHHDSGYFISVGDEFYLKQQKISTYLPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P141</accession>
    <name>A0A1E3P141_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">WICANDRAFT_94176</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="17">Component of the 850 kDa m-AAA protease complex, a heterohexamer composed of YTA12/RCA1 and YTA10/AFG3. Associates with the prohibitin complex, composed of PHB1 and PHB2, inhibiting the activity of the m-AAA protease complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038406.1">
        <property type="nucleotide sequence ID" value="XM_019186444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P141"/>
    <dbReference type="STRING" id="683960.A0A1E3P141"/>
    <dbReference type="GeneID" id="30203690"/>
    <dbReference type="OrthoDB" id="1413014at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005745">
        <property type="term" value="C:m-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097002">
        <property type="term" value="C:mitochondrial inner boundary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140567">
        <property type="term" value="F:membrane protein dislocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000019">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000003">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF2">
        <property type="entry name" value="AFG3 LIKE MATRIX AAA PEPTIDASE SUBUNIT 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="20">
        <location>
            <begin position="299"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="723"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="732"/>
            <end position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00065348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ59199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="745" mass="82500" checksum="1A52F8B89FDAC53F" modified="2017-01-18" version="1">MNTLRSRAALIGRPGFISSRLSTRISNISNVCLKRQPLLNQNVRSFSCYKPLAFQYSRFNAKDEDGKDKKNKKDGKKNNERDYQTMGEYFKSKEFYRSLYLTLGLTLVFYLITPGDSSAQNILTFQEFKSKYLEKGLVEKVYVVNKYLVEAELRSEAGVSSFGGKPSVAFTIGSVDVFEEQIDEIQDRLKIPPNERIPVAYVEKASILNILVPFLPTVLLLGGLYYLTKRVGQGSGGAGGPMSGLFNVGKSKAKLFNQETEIKTKFKDVAGCDEAKEEIMEFVEFLKNPQKYEKLGAKIPRGAILSGPPGTGKTLLAKATAGEAGVPFLSVSGSEFVEMFVGVGASRVRDLFKTARKMAPAIIFVDEIDAIGKERGKGGMGGNDEREATLNQLLVEMDGFESSDHVVVLAGTNRPDVLDPALMRPGRFDRHVSIDRPDIEGRKAIYRVHLKKLTLKINEDGFVEEKNDDLSGRLAALTPGFSGADIANACNEAALIAARYGDKFVELRHFEQAIERVIAGLEKKSRVLSKDEKKVVAYHEAGHAICGWYLEYADPLLKVSIIPRGQGALGYAQYLPPDRYLLSTDQFKDRMTMALGGRVSEELHFKSVTSGAHDDFKKVTNIAESMVKTLGMSDKIGYLSFDTSDQGGFQVQKPFSEKTSRIIDLEVKKIVDECYERCKNLLVEKADGVEKVAQLLLEKEVLTRDDMIRLLGPRKFKENADFEKYLGGGKSAPPPPPEETGPAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P149</accession>
    <name>A0A1E3P149_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_100903</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038187.1">
        <property type="nucleotide sequence ID" value="XM_019180310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P149"/>
    <dbReference type="STRING" id="683960.A0A1E3P149"/>
    <dbReference type="GeneID" id="30197556"/>
    <dbReference type="OrthoDB" id="10261640at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051179">
        <property type="entry name" value="WD_repeat_multifunction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857:SF8">
        <property type="entry name" value="ANGIO-ASSOCIATED MIGRATORY CELL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="58"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ58980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="410" mass="45135" checksum="E0FCED4BB16D6FFA" modified="2017-01-18" version="1">MSEEQDEFIGVNEVDEVYEGTNEPEPMSEDEDDVEELQNDQDQEEQEIDMSNNSWGYFDKHEDSVFTVFSHPTLPLVVSGGGDNTVYMWTSHSQPPKFVQQIKGHKESIITGAFTGDGEFLITGDMAGKIMIHQSTKRGQVWKLYAELEEIEEIVWISAHPKQPVFAFGGADGSVWVYQITPSVEQIMSGFAHQSESTGGVFVNTDDLDALHLVTISTDGSIVGWNGFTGQAEFKLAETEFKGQQLPWITLSPQGNSNILAVGSNEGTLAIININNGTVLTTFRVVELKEDQEELDASIETITWSDSLPLIALGLVSGAVILFDAKTWRERHSIILEDAITKLEFVKGTPYLIGSSMNGKIYKWDARTGQEIFAGVGHNMGVLDFSVVENGNKLITAGDEGVSLIFVTPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P181</accession>
    <name>A0A1E3P181_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MoaB/Mog domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_16780</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>adenylyl-molybdopterin + molybdate = Mo-molybdopterin + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35047"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>molybdopterin + ATP + H(+) = adenylyl-molybdopterin + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:31331"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58698"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MoeA family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the MoeA family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the MoaB/Mog family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037884.1">
        <property type="nucleotide sequence ID" value="XM_019180709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P181"/>
    <dbReference type="STRING" id="683960.A0A1E3P181"/>
    <dbReference type="GeneID" id="30197955"/>
    <dbReference type="OrthoDB" id="4349954at2759"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061598">
        <property type="term" value="F:molybdopterin adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00886">
        <property type="entry name" value="MogA_MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.190.11:FF:000001">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.980.10:FF:000001">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="entry name" value="MoeA, C-terminal, domain IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.10">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.190.11">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192:SF5">
        <property type="entry name" value="GEPHYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="MoeA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="MoeA N-terminal region -like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01079">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="4 5" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="7">
        <location>
            <begin position="3"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="MoaB/Mog" evidence="7">
        <location>
            <begin position="405"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="157"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="629" mass="68125" checksum="707A3A5569D78F81" modified="2017-01-18" version="1" fragment="single">KVGLLVISDTASKDNSEDKTSVKIEEFFANSELNPGYEIIKKDIIPDDIGQIQLKLRQWTETDELKLILTSGGTGFSDRDVTPEAVKPLIEKEASGIVHTMMSYSLKITPYAMMSRPVAGIRNKTLIVTIPGSPKGAVENIEGIIGVLGHALKQIGSAGSRHLHKSEPKAKEHTHSHSHGHHHHHHHHAHEGGHHGVKRHILSNDLSKPITQRARESPYPMIEVNEAFKLINEFTPSPQVEIKKINDPSITGYIVAEDIYANINVPNFRASIVDGYAVINSDGAGVYKVVQVSHASPSVSSTLQKGTIARVTTGAPIPDGANAVVPVEETELISTKDEGEEEDEVKILATNVEENDNVRGEGTDIAKGDLALAKGSQISPVGGEIGLLASIGVTSLKVFKKPKIGILSTGDELVDLGENCPTGELKFGQIYDTNRPLLLQTFRNWGYETKDLGIAKDTSKATEATIRKAFLEDDLDYIITTGGVSMGELDLLKPTIERQLGGIIHFGRVAMKPGKPSTFATIEVKGKRKVIFALPGNPASASVTSHLFVLPSLRKFSGVINLQDPKELPGFSKVNVTLTKQTKLDPRPEYQRVYIHQSTIGDELFADSTGFQRSSRVGSLAGANGLLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1I7</accession>
    <name>A0A1E3P1I7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ethanolamine-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CTP:phosphoethanolamine cytidylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_84757</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.14" evidence="10"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038317.1">
        <property type="nucleotide sequence ID" value="XM_019185773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1I7"/>
    <dbReference type="STRING" id="683960.A0A1E3P1I7"/>
    <dbReference type="GeneID" id="30203019"/>
    <dbReference type="OrthoDB" id="40021at2759"/>
    <dbReference type="UniPathway" id="UPA00558">
        <property type="reaction ID" value="UER00742"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004306">
        <property type="term" value="F:ethanolamine-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044608">
        <property type="entry name" value="Ect1/PCYT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780:SF2">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="12">
        <location>
            <begin position="13"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="314" mass="36223" checksum="910DA85605CD8960" modified="2017-01-18" version="1">MTMTELKEDRIWIDGCFDFFHHGHANAILQAKQLGSELYIGIHTDEEIKLNKGPTVMNLKERQYTIDANKWSTKTIINAPYVTQPSWMDEYQCKYVVHGDDITTDSDGNDCYGIVRDQGRLKLVKRTEGVSTTDLINRLLVPTTKHYIKDVGGMIDENKVLIKNFASGVDGKELYDGVWFVDNDEVKELYYKEMGPVVYVEGSFDLFNPYHISVLEELKLKGLEILVGVYRDGVEQTSMDLLQRSLCVLQCKYVDAVVIGRFNVSEGFVNEFDELNQNGISKRIQDHYELYVERQRKKGVKHELEQELEKNSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1K7</accession>
    <name>A0A1E3P1K7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_101076</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038430.1">
        <property type="nucleotide sequence ID" value="XM_019180313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1K7"/>
    <dbReference type="STRING" id="683960.A0A1E3P1K7"/>
    <dbReference type="GeneID" id="30197559"/>
    <dbReference type="OrthoDB" id="9986881at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF31">
        <property type="entry name" value="POLYAMINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
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        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
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        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="26"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="449" mass="49565" checksum="6801FB4850A26401" modified="2017-01-18" version="1">MYTVDFDGPDDPLIPFNWTIRKKLFVSTVVAVHSSLISWASAIFSPAIVTVGHRFGVSREVATLGLSLYVLGFATGPVIWAPLCELYGRKPIMVLSGIGMTLFQFAVATGKDIQTVLICRAFGGAISAAPLVCVPAIFGDIYRDETRGKAITVFIMTIFIFPIVAPVVGGFIVNSYLGWRWTEYLTGIMGSFTTLMIIFFYPETHHPILLVSKAHKIKELTGNWMIHAAHDEFTLTIKDIVEKNLSRPLVMLVTEPIILLISIYNAFVYGILYLMLSAYPVIFAEGYGMKGGVSELPYLGMVVGMVFAGVFAFLMEGKYVKALRANNHKPVPQARLYTLFYGCFAFPIGLFWLFWTGAYPKHVHWMAPTASGVFTGFGLVTIFTSSINFILDTYLAFSASAMAANAFLRAIFACAFPLFGVQMLHNMGVQWAGLLLGLIENPSFSKMEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1N6</accession>
    <name>A0A1E3P1N6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_79687</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038367.1">
        <property type="nucleotide sequence ID" value="XM_019184934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1N6"/>
    <dbReference type="STRING" id="683960.A0A1E3P1N6"/>
    <dbReference type="GeneID" id="30202180"/>
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    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710:SF243">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA-2 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="154"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="323" mass="36031" checksum="C68AA34A9922FB98" modified="2017-01-18" version="1">MSSSSENSSNFAGSNTPVTSQSSQTNFDGQNRTSGAQNIRVATDALAQHLESGNATLLEANTDNERSPDVLFRGSFTQGFPSFSVTIDRTNITSDGAVVGEPVFNFGNLPNNLRDMVQSLMESNRPRKKKATKDAIKRLKIIDPTTLKESERVCSICYDKFDQPKNEGRANDSKPITISINDTRELLTDDPPIIFPKDPTGSRYSEYHRLAKQSKHKDTGKEPEDNEPEGEHIPVQMPCGHIFGRSCLLEWLKSNISCPLCRREVEAQPVQATEEATADQRFFRNRSICPATWSASDNLYEDPEIPFPPTRRSRISIFNTALN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1X7</accession>
    <name>A0A1E3P1X7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13">Amino-acid acetyltransferase, mitochondrial</fullName>
            <ecNumber evidence="5 13">2.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Glutamate N-acetyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">N-acetylglutamate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_63414</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 13">N-acetylglutamate synthase involved in arginine biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>L-glutamate + acetyl-CoA = N-acetyl-L-glutamate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.1" evidence="5 13"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038114.1">
        <property type="nucleotide sequence ID" value="XM_019183543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1X7"/>
    <dbReference type="STRING" id="683960.A0A1E3P1X7"/>
    <dbReference type="GeneID" id="30200789"/>
    <dbReference type="OrthoDB" id="5585968at2759"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00106"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106098">
        <property type="term" value="C:NAGS/NAGK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006592">
        <property type="term" value="P:ornithine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011190">
        <property type="entry name" value="GlcNAc_Synth_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006855">
        <property type="entry name" value="Vertebrate-like_GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342:SF4">
        <property type="entry name" value="AMINO-ACID ACETYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04768">
        <property type="entry name" value="NAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007892">
        <property type="entry name" value="NAGS_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51731">
        <property type="entry name" value="GNAT_NAGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7 13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="14">
        <location>
            <begin position="358"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR007892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ58907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="519" mass="59431" checksum="B6AA6210DDC5014F" modified="2017-01-18" version="1">MLKSGKGVFRRLLSTKSLPFEGNLKRKSNDNKDLMLSILKLTTTKRETNNYLNKYSSFSKASNLDKSEIPPPLRLVVIKIKGNVLNYEESELRKFNKTLDYIQKLGASPLLLIDADFLHDRYDLNFSKIDNYLSDQYNYLNKCLEDKKLLTVTPVRCILSKNEQTDTELDIPEMILNPLKQDQVPILFPYVFAHKFGREVIYQSKDYLKVLIQNLSKLNSQTGNTLLTVEKLIFIDRLGGPPSIERGNASHVYINLNQEYERIMSELNIGHLSITEKASHIKNLEDLKDILTSTNNELTGIITTLPIANENLNLNPIIYNILTDRTLVSSSLPITKFQNKQDITTRVSKTSIIRGGLEIHSFKKIDDGIDIKKFKHLIDTSFKRSLNLDHYLSRIEPILSQVIIVGDYEGIAIVTNEKSPTTGEVIPYLDKFAVSREAQGSLNIADIIVNILRRDYKDGLLWRSRKVNPVNGWYFQRSDGSLNLQDSEFRIFWIGKGIDKVKLQTFVEIGKSIKPSWDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1Y8</accession>
    <name>A0A1E3P1Y8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_79467</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038129.1">
        <property type="nucleotide sequence ID" value="XM_019184896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1Y8"/>
    <dbReference type="GeneID" id="30202142"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="245" mass="27743" checksum="6EE06985CA2E63B3" modified="2017-01-18" version="1">MLKNITHQIRHFPRAECQTNKNSLCSELNTLSTLISKQLHSSAINASSPKGEKILQAIFAPKRQLRSKLPFKFPTKLSLRKEKPVYVTRSLYNDTKTEEWVCHDRSLPIEFSVKKGPKYTEVTQTKITNQSGPIVSLRGYGIIGERTVEGKLITKSRHYDDGRFTRSIVRKVRKVNKYDDGAVFTEERTETCVPDTDPNAPPGALIQTGELVKERSAPFKLKGDLYDNSCNNFLQISSLEASKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2A3</accession>
    <name>A0A1E3P2A3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein mS33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_63527</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS33 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038234.1">
        <property type="nucleotide sequence ID" value="XM_019183565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2A3"/>
    <dbReference type="STRING" id="683960.A0A1E3P2A3"/>
    <dbReference type="GeneID" id="30200811"/>
    <dbReference type="OrthoDB" id="2257454at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013219">
        <property type="entry name" value="Ribosomal_mS33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13362">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13362:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08293">
        <property type="entry name" value="MRP-S33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="82"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="108" mass="12752" checksum="83BCA8F251B513DA" modified="2017-01-18" version="1">MSSFLPPKSRLLELTKLRCNIFNENFNPLNIRTGSKILTQRLKGENIKRYYGNPDMIRFKELRSLYPTIDWVDPAEVYRLKINQSRKRRGKGAPKKKKEKGGDKKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2J8</accession>
    <name>A0A1E3P2J8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Trimethylguanosine synthase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cap-specific guanine-N(2) methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_14585</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78479"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="Rhea" id="RHEA-COMP:19089"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:78480"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78507"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="Rhea" id="RHEA-COMP:19090"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:78508"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78471"/>
            <dbReference type="Rhea" id="RHEA-COMP:19085"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:78472"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78475"/>
            <dbReference type="Rhea" id="RHEA-COMP:19086"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:78476"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. Trimethylguanosine synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038898.1">
        <property type="nucleotide sequence ID" value="XM_019180613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2J8"/>
    <dbReference type="STRING" id="683960.A0A1E3P2J8"/>
    <dbReference type="GeneID" id="30197859"/>
    <dbReference type="OrthoDB" id="194443at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071164">
        <property type="term" value="F:RNA cap trimethylguanosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017126">
        <property type="term" value="P:nucleologenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032210">
        <property type="term" value="P:regulation of telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019012">
        <property type="entry name" value="RNA_cap_Gua-N2-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741:SF32">
        <property type="entry name" value="TRIMETHYLGUANOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09445">
        <property type="entry name" value="Methyltransf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="235" mass="27032" checksum="6F20100850A26141" modified="2017-01-18" version="1" fragment="single">QDQLAQLQPESKYLLNYDRLPKNAKKYWKQRYFLFKKFDQGIHMTSELWYSVTPEDIGIFVAKFIQACNPDIQCVADICCGGGGNTIQFARRFKKTICIDINDDNLYCTKKNCEVYGVLEKVELRQVDWTKVKGTEYFEYLKQNVDLIFSSPPWGGPAYKGKDLFDLSLLLPMPIKELLVSFFEISPNVVLFLPRNSDLTQLAEVTREILGPDAKCRVLYTYCGGYIKGITAIWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2M5</accession>
    <name>A0A1E3P2M5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alcohol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_62190</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038810.1">
        <property type="nucleotide sequence ID" value="XM_019183322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2M5"/>
    <dbReference type="STRING" id="683960.A0A1E3P2M5"/>
    <dbReference type="GeneID" id="30200568"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000947">
        <property type="term" value="P:amino acid catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08297">
        <property type="entry name" value="CAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000002">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="10">
        <location>
            <begin position="17"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ59603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="348" mass="37683" checksum="D359C3AC92552B7C" modified="2017-01-18" version="1">MSLPTTQKAIIFEQHNGPLLYKDIPIPQPKPTEILINLKYSGVCHSDLHAYKGDWPEPTILPLIGGHEGAGIVVGVGSQVSNFKIGDYAGIKWLNKTCLSCEYCTLGEETCCENQDISGYTVSGSFQQYVVADAIQAAKIDPNVNLAEVAPVLCAGLTAYKALKKANLRQGQWCVIIGAGGGLGSFGVQYANAMGFRVIGVDSGDKKSLVLDELKAERFIDFTKTDDIVQDVVELTNGGAHGVINFSVSEIALNLGIKYVRPLGTVVIVGMPANSFAKSNVYEHVCKQFNLTASSVGTRQDTIEALDFFNRGLIKSQIVIAKLSELGKVYEQMERNEIQGRVVVDTYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2N3</accession>
    <name>A0A1E3P2N3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA replication regulator Sld3 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_78386</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038956.1">
        <property type="nucleotide sequence ID" value="XM_019184715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2N3"/>
    <dbReference type="STRING" id="683960.A0A1E3P2N3"/>
    <dbReference type="GeneID" id="30201961"/>
    <dbReference type="OrthoDB" id="5395343at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013948">
        <property type="entry name" value="DNA_replication_reg_Sld3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042511">
        <property type="entry name" value="Sld3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28067">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28067:SF1">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08639">
        <property type="entry name" value="Sld3_STD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication regulator Sld3 C-terminal" evidence="2">
        <location>
            <begin position="177"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="DNA replication regulator Sld3 C-terminal" evidence="2">
        <location>
            <begin position="245"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="633"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="420"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="437"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="655" mass="73703" checksum="590228CBE18A1952" modified="2017-01-18" version="1">MDQDQGVIGGHNIDGTFLISNNSVKDEPMALTLIKPIPYLLSDVSDNINHLQPSSKYQIINNNKSRIFRSNDDVLHQKVTGSMLVKLTVHSKDQYGVVSKLDAHNHFIINYLNTPMNAKSQESFQPLPYTPNIITQWSSTLSETKQNSFKLSMTPPIDNKSPIKNTALNEEGETIDDPFDYFLDRYFENLYLLTTPLTFFTKSTFTRLKTLCEGDNNEYESILKQFVIDHETFDNRHVMSNNGLLNSTLLYEKENIYRKKFIQKSLNLIDFDIHSSTSVETNKSLSNVLNNFKIRELHLQILLLIELIYVTDHDNVKHFKKLSKKPHLTKKSLVGRRKLTPTINGGAVPLEEQSVNKQVLNYNQILDIYIDKLCIWDVLMGTSSTDNSTPKFMSYIIVPYFNKKCPNSVKHMLKKIKGPSFKTSSSSSSKTNEKSSKSSSSSASLKRSASISSSNAEIKRPSLSRSSSNIKLEDIQELKTSLSRSNSDLQSLKRTSSFTANNLSKRQIDMSLPPIPSEPESQPLKRNSSIFTRVGKKLTQSITNNPTSSKSLSAPPIETFSQVDATPVKSVKYINPLLDQTPRDGENKVVTSSSPIEYAKTPQIMKTPAVIRSSVKKPGEPVDFNNAEEVEQMLGSPMGKGVRRRLFAPPTSQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2Q4</accession>
    <name>A0A1E3P2Q4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_84814</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038389.1">
        <property type="nucleotide sequence ID" value="XM_019185785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2Q4"/>
    <dbReference type="STRING" id="683960.A0A1E3P2Q4"/>
    <dbReference type="GeneID" id="30203031"/>
    <dbReference type="OrthoDB" id="269872at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006451">
        <property type="term" value="P:translational readthrough"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004556">
        <property type="entry name" value="HemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050320">
        <property type="entry name" value="N5-glutamine_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00536">
        <property type="entry name" value="hemK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18895">
        <property type="entry name" value="HEMK METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18895:SF74">
        <property type="entry name" value="MTRF1L RELEASE FACTOR GLUTAMINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="4">
        <location>
            <begin position="75"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ59182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="256" mass="28865" checksum="13478BB056F9BA84" modified="2017-01-18" version="1">MESASQELNWIKNELPKQQWLQACKQRSELVPLQYILGTQPFGHLEIKCRKNVLIPRWETEEWSHLLVELVKNIKNLKVLDVCTGTGCIPLLMADLIPHFNFKGIDISQDALDLALENKSSLGISNVEFVKGDVFDGNILGGTNFDLITANPPYVTEKSYYSKDTEESVRIYEPKLALIGDTEFYTALIENVVKPSGADGFIFELAEENQVQTTKKLLNSNWKVTTFTDSNGKLRCVFGYKVGSQMQILENLKSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2R6</accession>
    <name>A0A1E3P2R6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_78406</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038978.1">
        <property type="nucleotide sequence ID" value="XM_019184717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2R6"/>
    <dbReference type="GeneID" id="30201963"/>
    <dbReference type="OrthoDB" id="4036043at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="204" mass="23671" checksum="603AA1A5669318E3" modified="2017-01-18" version="1">MTDSPHTPINLINSNPTSNSTSIYNQLLKSPLNFDSDIDEQDESHHLLQFEQPFDQSPSSNSENVEIMISNLMIVLEDPQKDVNKQQIKTILQKCLITISHYKLQNEILRFENNEVNKRNDVENDLIKRQVDYLLKSPFKPSDESSITHEETTVQPKKQRRSSSRVKKPTTGAKNLKLVEKFNNHPDKLNEFNNCIKVFHLEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2T5</accession>
    <name>A0A1E3P2T5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_91399</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038860.1">
        <property type="nucleotide sequence ID" value="XM_019186293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2T5"/>
    <dbReference type="STRING" id="683960.A0A1E3P2T5"/>
    <dbReference type="GeneID" id="30203539"/>
    <dbReference type="OrthoDB" id="4356994at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052693">
        <property type="entry name" value="Yeast_MDR_Regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31405">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31405:SF8">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="644"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="33"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="696"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="719" mass="83274" checksum="575EA1CDAFBAAA91" modified="2017-01-18" version="1">MDAQNNNNFNRNNYTDHSSPDSNKKRRKRVSLTCTSCKKRKVKCDQGKPCSSCVKKRIPPHLCIYEDSPFIPNTEQSTTPTETIILLKDENVKLKAELEELKKWKEIVSGDPMPTPLGGKVQLFENISMKANKVMYFGPTSWRTLLDKEGTYTELVKSAKNYVSSVKREWKNEKNLNHIPYEEYKDINVGTPKTLLDNLANFLPKYDVLENYMIKYLDGYWNQRLPIIEREQLMKDFHQIVIPNGDDSGTFTFKIINKSIDYAKIALMVIVLKYMTATSNSHQQDSDYDSRDHLLRYADKLLEFSKSNSKSTIPALQALILIRQFRMINPIDGDGGDSSGGALTFKFAINMAILMGLNKDIDTLYAKSPPYTRQIFKNIWSHLMYQDAVLSFHLGIPLTIEDQYIDRHYFENNDLLSTITMFLREIVRVLTRSDHCSQHEIVSIIEKMENYNNGEFQKLSSSVKELSSADVVLNDFQKLISIELKLFAIYTLHVLYDVLYHGVEDYDPNKAVYYNGTMKYGTLSATHFIEILLRFNKFCVDNKDNEDKLFKIVEMSQSMVGVSKIIFIKIFCAICTFEVIRLFEDDLRSTHPKTLYFSMDITDFEKLNPHDRSHTLNQSYRSPAFLHGLMTLACKYMLKMKNESFQSVFNLNFCLFVVLALFKYFERFIDRKLSKEKETSSLDKIRATVKYEDCEDADLPHDDTSIPSPPAILQTSQDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2X7</accession>
    <name>A0A1E3P2X7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Maltose/galactoside acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_18580</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038817.1">
        <property type="nucleotide sequence ID" value="XM_019180795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2X7"/>
    <dbReference type="STRING" id="683960.A0A1E3P2X7"/>
    <dbReference type="GeneID" id="30198041"/>
    <dbReference type="OrthoDB" id="25818at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03357">
        <property type="entry name" value="LbH_MAT_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051159">
        <property type="entry name" value="Hexapeptide_acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024688">
        <property type="entry name" value="Mac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416:SF54">
        <property type="entry name" value="ACETYLTRANSFERASE, CYSE_LACA_LPXA_NODL FAMILY (AFU_ORTHOLOGUE AFUA_2G08430)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416">
        <property type="entry name" value="SIALIC ACID SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12464">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01266">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Maltose/galactoside acetyltransferase" evidence="3">
        <location>
            <begin position="3"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="191" mass="21277" checksum="06427BFEFB80891B" modified="2017-01-18" version="1" fragment="single">KDYELMISGNRYNCMNEQLLMDRLIRHEMAQDYGNLRMGSQSPEEYFQTRNEHLKKIFGKIKGDRGAYIEAPFQVDYGYNIELGKDFYANFNMTILDCSLVKIGDGVQFGPNVVLSCATHPVDSKERCVDFIEWAREIIIGDKVWLGANVTVLPGVTIGAGTTVGANSVVSRSLPAYCVAVGTPARVVKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3C4</accession>
    <name>A0A1E3P3C4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_68545</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ORC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039193.1">
        <property type="nucleotide sequence ID" value="XM_019184124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3C4"/>
    <dbReference type="STRING" id="683960.A0A1E3P3C4"/>
    <dbReference type="GeneID" id="30201370"/>
    <dbReference type="OrthoDB" id="10265211at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20704">
        <property type="entry name" value="Orc3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020795">
        <property type="entry name" value="ORC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045667">
        <property type="entry name" value="ORC3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040855">
        <property type="entry name" value="ORC_WH_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12748">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12748:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07034">
        <property type="entry name" value="ORC3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18137">
        <property type="entry name" value="WHD_ORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Origin recognition complex subunit 3 N-terminal" evidence="7">
        <location>
            <begin position="5"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 3 winged helix C-terminal" evidence="8">
        <location>
            <begin position="596"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="746" mass="86606" checksum="EE9827EEEBA85E3D" modified="2017-01-18" version="1">MDVSQFNESQKTVYVLRKPSKRQKIIESSQAVSEGAAVSINDPKVPFLRLLYGEEPIENVKLRYHLYQNSIQAQDTIAKKILDETSDQILTNISNIINLPINNEQLDSVLLLSSSSFTSSSSFISQLTSHLLNGDHNNHRVVRLSSKECNTIKVTLKKIVSSILYDTLADGDNFIDNDSDEEENNVSDESEEEEDDDDDILLQHKRLPFDLDSIVEWCQQIYSKKNESIDTTSTRIVLLFEDTDSFNIGLLNHLIKLIHRFTNRIPFRLIFNIGTNVENFSKKLDNELHFLLKFHQFKIEQTGLILNKIVSDIILQGDFLIGNESIYFLLNRFQNSTRDVNEFLKCLKFLKMSFFFNQPFAIIGEILQRQNGEILLNDSYYAAIKNTESFRDYINQSINSDQYDPDEILALINQDNKDNDLISKLVIKATNDYIKNLSNFKTVFKIITNVLLKHVSISKQTYLEILAKLTNKDELVRNQLLNEILTSLKKLEIDALEDLLHNINEEAANNDLLQSLNSKVKAVSIDLTALKETLNDPESSKIQFKIDYRKLLESLISTIDFFLKKSFKNPFELLFNEVFQLNDLDILKSTIIPQTRTIIEDSLTDPELYINMNNKSQTQDPLERLNIILNPIICELFQIYRETSLNLNIYDFYQVFKNSLRSEEIMKLFMEILDNTDLLEKYLNDADRFKFIYIFFNDADISNEMKFDKLMLVFFLQKCGELIDCGFLKQIKRKGDSLEKCIWRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3W2</accession>
    <name>A0A1E3P3W2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_21398</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039388.1">
        <property type="nucleotide sequence ID" value="XM_019180903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3W2"/>
    <dbReference type="STRING" id="683960.A0A1E3P3W2"/>
    <dbReference type="GeneID" id="30198149"/>
    <dbReference type="OrthoDB" id="361494at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070912">
        <property type="term" value="C:Ddb1-Ckn1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042238">
        <property type="entry name" value="Rad28/ERCC8/Ckn1/ATCSA-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202:SF1">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="57"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="90"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="176"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="246"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="387" mass="43121" checksum="5836A96695954154" modified="2017-01-18" version="1" fragment="single">MQSHLLDRQLGKISTRSFELLATESLYYNLESQDYPKFTQKKKTIGSINALDFDKIDHKFLLSGGADSTIKIWKVGSQKAELVGDLNKSNGGHEFGVTDVKWWPFDTGMFLSSSYDMNLKVWDTNTMEEAFNFNLDSKVNCFDVSSTGAHSLIATGADSSHVRLLDLKTTSAIHSLTGHNGCIVSTKWSPNNANVLATGSSEGEVRIWDIRRTDSCLVQLDLYRTDEKAPLSSNDSRKVGVLKSSVKAHARGANGLCWLESGKTLITTGNDEKIRVWNLEATGGVNTLLNFGPFVRNRYSSYKSILLSPMEETELQYLWYPSDNGEILVFRIVDGKLVARLRKNLQENSRSCCIAYAGNNSATYYSGTVDGKINVWGPKDERSFEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4L6</accession>
    <name>A0A1E3P4L6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_92579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039500.1">
        <property type="nucleotide sequence ID" value="XM_019186370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4L6"/>
    <dbReference type="GeneID" id="30203616"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="62" mass="7261" checksum="32AF9273823E8D1D" modified="2017-01-18" version="1" fragment="single">MSEEFGKDDNEVQINDNSIVETWTEIMDGYRKIHAKKDSEEIELESISQEPEEKSRSEDVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4N8</accession>
    <name>A0A1E3P4N8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycoside hydrolase family 5 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_63013</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039666.1">
        <property type="nucleotide sequence ID" value="XM_019183476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4N8"/>
    <dbReference type="STRING" id="683960.A0A1E3P4N8"/>
    <dbReference type="GeneID" id="30200722"/>
    <dbReference type="OrthoDB" id="1887033at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046557">
        <property type="term" value="F:glucan endo-1,6-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000100">
        <property type="entry name" value="Glycoside hydrolase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GH"/>
    </dbReference>
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        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF43">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="6">
        <location>
            <begin position="77"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ60459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="500" mass="56887" checksum="55DAB0962B6DCC86" modified="2017-01-18" version="1">MVLDKLKSLAIDQESLKAAAPAAPAGKPPSKKQIYQSRYNFGVNFGSLFVLEKFIFDKFFIDNTSCELEAITAYSKKNGAENTQKELEDHWNNYATDQDWEWLKSKGVTAIRIPIGYWHVNGGSFASDTPFAKVAGVYKNAWSIFKNIVAKAKQYDIGVLIDLHALPGGANAAEHSGQQLSKAEFWKSKKNEKLALSVLEFIAQEFKNEENIVGLQIVNESEFDNTAASQKHYYTKAVKHIRNIDPEIPIVISDGWWPDQWVKWVVENEQDLKNQSVGIVIDHHVYRCFSDADKKKAPEQIIKDLNGDLLTNLSGQADVMVGEYSCVLDGDSWNKAGGDRNELVKQYGNELSKLFYQRAGAGSYFWTFRFEHGDGGEWGFVPQVETGAIPSRPTQVTQVPPEDEVNKILENELNQHSQYWDGQNKNEKYEHWRYKEGFITGWADANEFSKFDGSTIGRINAWKIARRNEHISHRKNSNFLWEWDQGFDKAVSIFNQVAFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4P8</accession>
    <name>A0A1E3P4P8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein BCP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_54399</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BCP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039676.1">
        <property type="nucleotide sequence ID" value="XM_019182736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4P8"/>
    <dbReference type="STRING" id="683960.A0A1E3P4P8"/>
    <dbReference type="GeneID" id="30199982"/>
    <dbReference type="OrthoDB" id="27543at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025602">
        <property type="entry name" value="BCP1_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261">
        <property type="entry name" value="BRCA2 AND CDKN1A INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261:SF0">
        <property type="entry name" value="BRCA2 AND CDKN1A-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13862">
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    </dbReference>
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        <property type="entry name" value="BCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="251" mass="28824" checksum="EF887B1129FD9BC5" modified="2017-01-18" version="1">MSKRRIEEEEESDIDISSTDSENSDQEEEEEEIINVDFDFFDINKDIDFHAIKNLTRQLLGEDGKKIQISPLADLILEAPTITIKTDGKDSDPYAFLTALNLKDLKTNDYLKYIHKSDAKLSAYLNKISHKNNALLLGERMINMPIQVIPAIFKLTLEEAEKANGAHFDHYLIPSRKYEVNDETEENSNKRIRTVEVDYYHFEDKFLEDNASFKTQLESRGGIIQSFIVVSHEGVIKAIGELEDVITSMFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4Z2</accession>
    <name>A0A1E3P4Z2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NADH-ubiquinone oxidoreductase 12 kDa subunit, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_62956</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039605.1">
        <property type="nucleotide sequence ID" value="XM_019183465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4Z2"/>
    <dbReference type="STRING" id="683960.A0A1E3P4Z2"/>
    <dbReference type="GeneID" id="30200711"/>
    <dbReference type="OrthoDB" id="10252718at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039993">
        <property type="entry name" value="NDUFB10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13094:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13094">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE PDSW SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="11199" checksum="84AFEE723F3FC97F" modified="2017-01-18" version="1">MAQEHIRPELVSFDNIDYEDPIALRSAQESMLREQWIRSNALRVCRRALEKCYRHHGVNHYEECRDLAEKYMQMLPTHKVKGFYGYQRNDPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P539</accession>
    <name>A0A1E3P539_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_29695</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Essential for proper morphogenesis of the vacuole. May exist as structural reinforcement on the surface of the vacuolar membrane and be required for maintenance against rupture by osmotic pressure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097576">
        <property type="term" value="P:vacuole fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06897">
        <property type="entry name" value="PX_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15858">
        <property type="entry name" value="SNARE_VAM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000058">
        <property type="entry name" value="VAM7p Vacuolar SNARE protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
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        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431:SF14">
        <property type="entry name" value="LD15323P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431">
        <property type="entry name" value="SORTING NEXIN 17 AND 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="PX" evidence="7">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="6">
        <location>
            <begin position="277"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="273"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="340" mass="39192" checksum="70CB94B78634407D" modified="2017-01-18" version="1">MDVKVTIPNTSLSDDGSHTVYHVLVKTAKKSHKLQKRYSDFAALKEELESLYSDQIPYQFPKKTFLRKTINNERLAEERRLQLEKFLQDVINDQINIKWRRSLPFREFLNLPPGAFSTVNESTKEKLDTAWTLSSTNNDEPIVDLTTWIDTVRETKNVLQDARSKIFINSVEARKKLILGRQKFDGLTKGLDVAISQSAIGEGESQRRRELLHSLKREHQDLEQLLSNVSSNPNKVDIRTPVGKSKNQLFKGRVLGKPQETERTRGVENQQLLQMQRNDMQEQDQELERLSVVINRQKELGVAINEELALQNELLDELDTEVDRTTAKLNYASKKIGKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P559</accession>
    <name>A0A1E3P559_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Superoxide dismutase [Cu-Zn]</fullName>
            <ecNumber evidence="8">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_83973</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="8">Binds 1 copper ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="8"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039184.1">
        <property type="nucleotide sequence ID" value="XM_019185632.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3P559"/>
    <dbReference type="GeneID" id="30202878"/>
    <dbReference type="OrthoDB" id="2015551at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902693">
        <property type="term" value="C:superoxide dismutase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016670">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901856">
        <property type="term" value="P:negative regulation of cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00305">
        <property type="entry name" value="Cu-Zn_Superoxide_Dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000001">
        <property type="entry name" value="Superoxide dismutase [Cu-Zn]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018152">
        <property type="entry name" value="SOD_Cu/Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00068">
        <property type="entry name" value="CUZNDISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00087">
        <property type="entry name" value="SOD_CU_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00332">
        <property type="entry name" value="SOD_CU_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6 8" id="KW-0186">Copper</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="9">
        <location>
            <begin position="14"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="154" mass="15736" checksum="0D0C8281C42FE65C" modified="2017-01-18" version="1">MVKAVAVLRGDAGVSGTVTFEQASESDPTTISYEISGNDANAERGFHIHEFGDNTNGCTSAGPHFNPFSKTHGAPSDETRHVGDLGNIKTDSNGLAKGSITDKLVKLFGPTSILGRTVVVHAGTDDLGKGGHADSLKTGNAGGRPACGVIGFQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5H1</accession>
    <name>A0A1E3P5H1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U3 small nucleolar RNA-associated protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_61261</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039902.1">
        <property type="nucleotide sequence ID" value="XM_019183144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5H1"/>
    <dbReference type="STRING" id="683960.A0A1E3P5H1"/>
    <dbReference type="GeneID" id="30200390"/>
    <dbReference type="OrthoDB" id="4055624at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018843">
        <property type="entry name" value="Utp8_b-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053881">
        <property type="entry name" value="Utp8_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10395">
        <property type="entry name" value="Utp8_b_propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22542">
        <property type="entry name" value="Utp8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Utp8 beta-propeller" evidence="1">
        <location>
            <begin position="2"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Utp8 C-terminal" evidence="2">
        <location>
            <begin position="357"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="563" mass="63470" checksum="D890D5CCD12DF07F" modified="2017-01-18" version="1">MSLSEPYTLAKLPRVSGTSERCEVSRNSDSEALHVGISGASISNYVLKPSPKLIWSHSIPPSSIITSLENDDDQYYVGVYNKTKKTHSLQVIKKLANDSELVKEVEIQSKIINIKSFTNSTIIITEDSVISFDPAFEVSWESKNLYKAIYSEFIEKDVILVVEHQAKKNNLNYRLLSVTGSEVNSKIYENKAKSTDLKFTYSEGVLFQYVNNSIVLYQLPHFQETKTLTLDQLSIKAPSSSKFSFESPAPDRLLLIIDQDFYLINTNFNIVLSTTSSTKSKAEILSTTKAASKNSRASLFGIVLRDGDIAGVPITLDSNTLKDSLGKRPSPNDETFKVVPSIFDIKDEVVDIDSIINRKDFDSALLTFLEAENDYYTEKDKVVDSKFIKSIVSHIFKQNELPERAMTYLLTHPLFPTIDGLLSLLRSKPRLLRQAIVTANVSIKELNQELNTTENDEIFKDIITRLLEFPKDKLNFKDLDSFKIVERIISLDYGYELISLLIDASGLFTWSDDLIIKLQEVLSKKIEALDSGSNALAVIEQIELKHLKTVKKVPVYSIEKLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5J6</accession>
    <name>A0A1E3P5J6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">5-oxoprolinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_77199</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxoprolinase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039721.1">
        <property type="nucleotide sequence ID" value="XM_019184515.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P5J6"/>
    <dbReference type="GeneID" id="30201761"/>
    <dbReference type="OrthoDB" id="3643at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF2">
        <property type="entry name" value="5-OXOPROLINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="4">
        <location>
            <begin position="6"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="2">
        <location>
            <begin position="234"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase B/oxoprolinase" evidence="3">
        <location>
            <begin position="733"/>
            <end position="1246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1290" mass="140529" checksum="76446D428C33270F" modified="2017-01-18" version="1">MTQGIKIAIDRGGTFTDIYASIPGKQDLVFKLLSVDPNNYPDAPTEGIRRVLETAHDSKIPKGTLLSVDTIESIKMGTTVATNALLERNGAKVALLTSKDFRDVLEIGNQTRPDLFNISARKLDQLYSKVVEIDERITLVNYTEGGGGLEHETNADAEAITGDLIRIIETPNISKVKLQLEELKAEGYSSLAICFLHSYLYPKHEQQVAKVARSLGFQVSVSTELQPTMGIVDRCSSTVADAYLLPKIQEYLVGFGSGFQGGLKAMGNKLLFMQSNGGLCPWNKFTGLRAILSGPAGGVVGYAETCYDKRPSNKKSTLGFDMGGTSTDVSRFSGRYEHVFSNVVNEVHLTTPQLDISTVAAGGGSMLFWKNGMFVVGPQSAGAHPGPACYRKGGPLTITDANVFTGRLLPKHFPHIFGPNEDEPLDVERTRVLFEELTTVINNELKSKMTPEDVAIGFLKVANEAMSRPIRTLTEGKGYNTADHNLAVFGGAGGQHSCAVARVLNIENVLIHKYSSLLSAYGISLADVVVEKQTPISIVYNQENLETIKTKLDDLDKVVESEASEQNVGSSPVSTELYLNMKYLGTDTCLMTLKPEVGWDFKSQFISQHQQEFGFTLEREVIAEDARVRKIIRSNDGNKLNPFEESEYVVKTNAPQPLESTKVYFENMGYLDSNIYKLGSLSTGTMITGPSIIIDDTQTILIEPDCEAVVLSDCVMIKLTNTKSTKLSTTIVDPIQLSVFGHRFMSIAEQMGKIRLFSALFNSDGKLLANAPHIPGHLGSMFLAVEKQLGIWKGQLKKGDVVFTNAHTTGGTHLPDITVLTPILDEHNNVLFALCARGHFADIGSIVPGSMPANSKELYQEGAIIESELLYKEGVFQHDKVVDLLYTKPGQYPGCSGSRLLDDCISDLKAHASANNKGLILMQNLINEFGLDVVHLYSNAIQKSAEAAVRDLLKIAYDRFGGEPLKAVDYLDDGTAIAVTITIDREDGSAILDFTESGDQFYSNLNCPIAVLYGSILYVLRCLINVDIPLNSGCMQPLDIRVREGSLFMPSPDAAVAGSNVETAQRISDTLFKAFQACAASQGTCNNFTFGSSDPQYGSFGYYETIAGGGGAGENFHGQSAVQVHTTNTRMTDSESFEKKFPCILRKYEINRGTGGKGLYKGGDGVIREIEFTIPIEATCLMQRRVFAPYGMNGGHPGKRGYNYWLKKLDDGSFKRVALGGQNTVFVKAGDRIRIETPSGGGWGVPIDEQDDDKKSISEQIHYPCIDAPFAKPTILTGSIGMKAITQTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5L9</accession>
    <name>A0A1E3P5L9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small nuclear ribonucleoprotein Sm D3</fullName>
            <shortName evidence="9">Sm-D3</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">snRNP core protein D3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_19983</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the snRNP core protein family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039948.1">
        <property type="nucleotide sequence ID" value="XM_019180850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5L9"/>
    <dbReference type="STRING" id="683960.A0A1E3P5L9"/>
    <dbReference type="GeneID" id="30198096"/>
    <dbReference type="OrthoDB" id="6425924at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036261">
        <property type="term" value="P:7-methylguanosine cap hypermethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01721">
        <property type="entry name" value="Sm_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000002">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034099">
        <property type="entry name" value="SmD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="7"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="91" mass="10218" checksum="B13A9C2ACC8944E5" modified="2017-01-18" version="1" fragment="single">LNMSAGIPVKLLNEAQGHIVSLELNSGETYRGKLIESEDNMNVQLSDITLTSREGQVTHLDHVFIRGSNIRFFVVPDMLRNAPMFDPNHVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5T0</accession>
    <name>A0A1E3P5T0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Very long-chain fatty acid transport protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Very-long-chain acyl-CoA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">WICANDRAFT_30374</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Acyl-CoA synthetase required for both the import of long chain fatty acids (LCFAs) (C14-C18) and the activation very long chain fatty acids (VLCFAs) (C20-C26) by esterification of the fatty acids into metabolically active CoA-thioesters for subsequent degradation or incorporation into phospholipids. The transport and fatty acyl-CoA synthetase activities are genetically separable and are thus independent activities. Esterifies VLCFAs in the peroxisome matrix. The VLCFAs are actively transported into peroxisomes by a PXA1-PXA2 heterodimeric transporter in the peroxisomal membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a very long-chain fatty acid + ATP + CoA = a very long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54536"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58950"/>
            <dbReference type="ChEBI" id="CHEBI:138261"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039404.1">
        <property type="nucleotide sequence ID" value="XM_019181727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5T0"/>
    <dbReference type="STRING" id="683960.A0A1E3P5T0"/>
    <dbReference type="GeneID" id="30198973"/>
    <dbReference type="OrthoDB" id="10253869at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031957">
        <property type="term" value="F:very long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044539">
        <property type="term" value="P:long-chain fatty acid import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000038">
        <property type="term" value="P:very long-chain fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000020">
        <property type="entry name" value="Long-chain fatty acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000019">
        <property type="entry name" value="Long-chain fatty acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107:SF15">
        <property type="entry name" value="FATTY ACID TRANSPORT PROTEIN 3, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="13" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="20">
        <location>
            <begin position="64"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="21">
        <location>
            <begin position="521"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00068795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="ODQ60197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="648" mass="73202" checksum="1BC405A25C840D30" modified="2017-01-18" version="1">MSKAFDYVKSLSSSIVGLGLQGFSNLDEKTHIADDMYIIQFVIKKAIGHAYSILTGQVSYWYIFENVVKQHPNKLALVYPKPVPGQTGDGAFQLESYTFQELYDIILRLSEILAHRYGVKEGDTIGLDATNKPLFIFLWFALWNLGATPAFINYNNIGNPLVHCIKVANISQVFIDPAAASPINETKDDIIKECPDVALHFLDEDKLLDEIKDPNSIKFRPATRNANDPDYATGALIYTSGTTGLPKPAIMSWRKACLGSALYGHIVRIKTNSVVFTSMPLYHSTASVLGVCTTFNQGAAVALSPKFSTSTIWTQIKLTKATHLQYVGEVCRYLLNAPIHPDERSHCLQVAYGNGLRRDIWKEFKDRFGIHAIGEFYAATESPIALTSFQEGEYGIGACRNYGKLINYILSYQQTLIRMDPEDNSVEYRNSKGLCELTRVDEPGELIMKLFWAKSPEKVFQGYLGNKKETESKIIRNVFRKGDAWFRSGDLLKSDANGLYYFVDRLGDTFRWKSENVSATEVENQFLQLHDILQIVVVGVKVPNHEGRAGFAVVELKEGVTKTEDELLSEFLEVLQKELPRYAIPIFIKFVDKIQESDNHKVPKKLYRDQKLPKGENGDETIYWLKRGKYEILSESDWKEIISGKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5Y0</accession>
    <name>A0A1E3P5Y0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_61415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040056.1">
        <property type="nucleotide sequence ID" value="XM_019183172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5Y0"/>
    <dbReference type="GeneID" id="30200418"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="287" mass="32920" checksum="C8264DC9950CBE3A" modified="2017-01-18" version="1">MPNFKTPTKMQSSSFYIVHTKPNTELVGSFSEYPSQAKTRNSENEDNSLNLPIKSKTHGFQLSKALKYNHSTFVIQSQDVKKKLFKNNLLSYGIPKTEKTKEKLVNLHFPFSINIIKLPTVTDEQIFNYILTNNITEKKYIQYSSFKNDKFPLFIFDNRSELPNVEVVGNMIKADVTSISKINGKPHKNKVYLRVLNKMKYKTVSNSKNACDQTFLISSDRSLICSKVGITNAISLFIRDFHKGEVSSKDYDYSTSVVPSVEEIKPNPPSYEDALEQCEPPRYRENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P687</accession>
    <name>A0A1E3P687_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_104059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040090.1">
        <property type="nucleotide sequence ID" value="XM_019180393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P687"/>
    <dbReference type="STRING" id="683960.A0A1E3P687"/>
    <dbReference type="GeneID" id="30197639"/>
    <dbReference type="OrthoDB" id="4138492at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="512" mass="57492" checksum="D78AF6AFC6CB94FB" modified="2017-01-18" version="1">MLKLVAIVAVGVLLAVLAYSKYLDSQKDIHELALSEQSNISPTRKNDETAIYRNNQTPHGLPLLTGLQIRHGFKLRNGNLRDIWSIVMGQKNINSVTFIENGNEVKADAYHLNGIFEKLNLFFKDHEISTVGVAVPLYSFHGFVSSFTCFVNGLTAHHFNHLPRTNPDVDVLMVDEKNLPFALRLDIKLIIVVSQDPLKSFSQQNVINWNELGDLTSLNSDTYSYNYDPEYDSGVPFKDTFNFATTDYSHQNFVSAIASVARSLPLGHELSHNDTLLIGYDDSSVKYWAKIFSVLLFGGSIVVGSSSTLKFDETLIEKYRPTILAIDSKTAKRFFLKHEPKGIIHSLLVSRVNYLLSRGIFSTMAALNEYKSLRLIYIDQLNSSNENNSSTELNQIRSLTGARVISERLIPGVIGSILNTNFYDYRVFTNSKLKNCGTSSLSLEIKLYKYNGLNIEKRHGELCIRGFIIGKPNDEKNLEEAIQKGERVGSEGWMPTGIIGKFGVDGCFYEDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6B3</accession>
    <name>A0A1E3P6B3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone acetyltransferase type B catalytic subunit</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_61519</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040166.1">
        <property type="nucleotide sequence ID" value="XM_019183191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6B3"/>
    <dbReference type="STRING" id="683960.A0A1E3P6B3"/>
    <dbReference type="GeneID" id="30200437"/>
    <dbReference type="OrthoDB" id="10253098at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.360.10">
        <property type="entry name" value="Histone acetyl transferase 1 (HAT1), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019467">
        <property type="entry name" value="Hat1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037113">
        <property type="entry name" value="Hat1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017380">
        <property type="entry name" value="Hist_AcTrfase_B-typ_cat-su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013523">
        <property type="entry name" value="Hist_AcTrfase_HAT1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12046">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE TYPE B CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21184">
        <property type="entry name" value="HAT1_C_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10394">
        <property type="entry name" value="Hat1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histone acetyl transferase HAT1 N-terminal" evidence="9">
        <location>
            <begin position="8"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ60959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="389" mass="45665" checksum="2B7DA29609648CF4" modified="2017-01-18" version="1">MSYNPEVWTISSNEALKVSIADDQGYVSFPPLFTYPIFGDSEQIFGYKDLKIDLVFDSITFLPFLKVSYKEKLNDEVDDITAKLLEFLPKSTIVDDEVKWVDAFKKEQEELLDFNKEFELINTYNVKNEEFSIFKTSFQTSEGEKTKAVDLMKRLQIFVLLFIEAGSYIDTSDELWDLFILVSNKTKKILGFSTAYSYFKYNGAIEFDSNPNVKKRNKISQFVVLPPYQKTQHGSSLYNSIISYWNGNELVEEITVEDPNEKFDDLRYRNDFKRLFEEGFINQLPKNLKTIDNKWFITNANKFKIELNQFKKLVEIGYLYTENIKLARLLIKKRLYEKNRDGLVELELAIRNDKLQTAYESIKQEYDRLIDSMRVRAVNDIGSSKKQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6F9</accession>
    <name>A0A1E3P6F9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_19982</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040218.1">
        <property type="nucleotide sequence ID" value="XM_019180849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6F9"/>
    <dbReference type="STRING" id="683960.A0A1E3P6F9"/>
    <dbReference type="GeneID" id="30198095"/>
    <dbReference type="OrthoDB" id="440755at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF46">
        <property type="entry name" value="BLR6921 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="24650" checksum="E0ACE907139BEF56" modified="2017-01-18" version="1" fragment="single">YIPLVIGVLVFFSVVIFEMGYIQNYKDKVKGSNDWRYTIQLLFPREVITIPNFFQYLVSTFLFYIDFIAIMSTMIQYNLYITLDPPILAAVKVLPVPLGLTVGALLYKEKTAQRIGTKFVIMASPILLLTMTVWLSRVDFRKSHNFWKYQCFSELIFGFGINLYFQVYWNDILTGTPVHLQGVVSGILQTAGQIGICFGNTIIAALVGDLTLNHDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6Y8</accession>
    <name>A0A1E3P6Y8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxisomal membrane protein PEX17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_104233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040325.1">
        <property type="nucleotide sequence ID" value="XM_019180406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6Y8"/>
    <dbReference type="STRING" id="683960.A0A1E3P6Y8"/>
    <dbReference type="GeneID" id="30197652"/>
    <dbReference type="OrthoDB" id="2357318at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055334">
        <property type="entry name" value="PEX8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39214">
        <property type="entry name" value="MICROBODY (PEROXISOME) BIOGENESIS PROTEIN PEROXIN 8 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39214:SF1">
        <property type="entry name" value="MICROBODY (PEROXISOME) BIOGENESIS PROTEIN PEROXIN 8 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="633" mass="71511" checksum="650A99850FA5F6B2" modified="2017-01-18" version="1">MYRLGSQGRAAQQRLQQQPYSSTVEFQTPSSQAQNDVNLLLSALKTPGNLSSTNLLSYIVHYLPRVRNEGNLKLLMDAFFSNRVIFPEQIHLEETYMIIEAIKATFDSKIRVSEPTLPLTKFFHGVVTSIFESGVEPWKKILVITGVILSQSTYQEQSIPETKHYFQTTYGSLINLNHELIMRVFQTTSINSEEVNALTAISLSCSLPFFSPSQRQHVPHESILLFTIHLIFQSPYGLGNHSTEFLAGSNSPVVKHLSRLSFLIENSLVHGVSFGVLDSTLNIILRFSETLHLRSANTDQSWNVLKNGIFAVVIIFQGFVSYLLKLSNRLSKQHYSSIATKILQTFFHLSFILEKIGTGGFQAYNFVYISSLDALLQYSHQSAEALGSYFTKNINLHSNDHYQVSKTLFTLTFFETLCQTTSIEYYENTISPIVDFIIHTTTHSRPLIEAAHSVVIATFIPTNSHIIASTAIPYLHTVLSQFPQLLSATQLRIAIETIARAVAPPSEAHQLNKDNLREVLHTLYIRCLNTRPGLPIPTSQPISQGQESDNNQPMTVRTGIIAALISTLPYLPTQILNNWLNNIWELTESSDPIEFQFCEKNLWEMISEGFDMQRGNVGINWWYNRGNNVEAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P702</accession>
    <name>A0A1E3P702_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">pyridoxal kinase</fullName>
            <ecNumber evidence="2">2.7.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_61567</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pyridoxine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.35" evidence="2"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040211.1">
        <property type="nucleotide sequence ID" value="XM_019183198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P702"/>
    <dbReference type="STRING" id="683960.A0A1E3P702"/>
    <dbReference type="GeneID" id="30200444"/>
    <dbReference type="OrthoDB" id="3976101at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008478">
        <property type="term" value="F:pyridoxal kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009443">
        <property type="term" value="P:pyridoxal 5'-phosphate salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01173">
        <property type="entry name" value="pyridoxal_pyridoxamine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004625">
        <property type="entry name" value="PyrdxlKinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00687">
        <property type="entry name" value="pyridox_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534:SF2">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="8">
        <location>
            <begin position="80"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="9">
        <location>
            <begin position="284"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ61004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="812" mass="92861" checksum="EAA7DD911CE00B6D" modified="2017-01-18" version="1">MTKESQRIHKLLSIQSHVVHGYVGNSAATFPLQYRGWDVDALNTVQFSNHPAYGSFKGTRSTAEELKEIYQGLNEIGTDYDAILTGYIPDDEGLKAIGEICIDICRKNHIKWILDPVLGDNGKIYVSEGNIEIYKELLKSGYVNLVTPNQLEVEVLTGVKVNDLTSLKKSISEFYNQYPGIENIVVTSVQFDDDDDHLYSAGSTKINSSTKKGFYFKVPSIKASFSGSGDLFSGLLTSAFFKYYDKFEGELNELTPIERLPLLNALNEVLSIVEKVLVLTYDVVLSRILSFLPQSDVCSTAQCSKKLHHVSLERLYKRICIRKDPVYHSNIWYIDCSWTTVSGYRSLHKREDQNDYIIHLKLDRLIQSLQNVKYSSLVKEVIILDKVFNYEDSNEQLLDLIETLEDKAKNIEVLDVPDSLLKAVSITSPKKIVTDDIDQIKETKNLKSLTIQLHSNTEERFPSVLPSLNNLEELILIDEESASLRILKTLSEVGLQKLKLKRLAFNHVHGLHDYNAVLRNLTITFVEECVDLGSLEELEMAIGCEVKNCDCLDSFLDDLAPKLTILKKLSIQEKTFHRDHYLSEKWDVNINRFLLHLPPGLKFLSIRHNPPRDGKLENNVEGNYVRRRKLYEKVLPTLKSLEVLASPTLLQTCACYEILVSDLLWNGCECEYCSEVLNLFDHYLMNHQYYDEEDADFRDVIAPRLFGIGGVELLKRLKNQSDLDVLKFGPGETTWDFHGYESINHFNDYDCMFDESFFEPLAVCVSHFYKGYLEFFEQNLPNLKTVVLSGIFFTIGPDRTIKCIYDNPKPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P710</accession>
    <name>A0A1E3P710_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_89817</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039854.1">
        <property type="nucleotide sequence ID" value="XM_019186196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P710"/>
    <dbReference type="STRING" id="683960.A0A1E3P710"/>
    <dbReference type="GeneID" id="30203442"/>
    <dbReference type="OrthoDB" id="5418899at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="201"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="275" mass="30893" checksum="AD1F017D3B9737F5" modified="2017-01-18" version="1">MVMGPTPSVLQWSGYRPAPQTKPLNESSDLFPTISTSEAIVNPQQQNGSITNIAQSPVNQIQHQHSQQHHDFNSPTSTNSSFSQAKNQSFDDYRYSHTSTNWLYGPGANVHPGHQHPEFQLDPQHNTTSFDDVGTNFNGEEASKPRTESSLTSMSSPEYSSLLEDDNGSPKSMRGRSSSTQSAKGKVHKDATGKTKRSRMGCLTCRHRKKRCCERRPKCQECERLGLKCTWPVPGLEHRNRPKNAKMNEDMHYDAFYGPIKILRGIVEYRINATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P711</accession>
    <name>A0A1E3P711_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription factor IIIC 90kDa subunit N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_104325</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040428.1">
        <property type="nucleotide sequence ID" value="XM_019180412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P711"/>
    <dbReference type="STRING" id="683960.A0A1E3P711"/>
    <dbReference type="GeneID" id="30197658"/>
    <dbReference type="OrthoDB" id="6021743at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024761">
        <property type="entry name" value="TFIIIC_delta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024764">
        <property type="entry name" value="TFIIIC_Znf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12657">
        <property type="entry name" value="TFIIIC_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12660">
        <property type="entry name" value="zf-TFIIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117289">
        <property type="entry name" value="Nucleoporin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor IIIC 90kDa subunit N-terminal" evidence="1">
        <location>
            <begin position="21"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor IIIC putative zinc-finger" evidence="2">
        <location>
            <begin position="514"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="584" mass="66191" checksum="053C64A93AD5BC50" modified="2017-01-18" version="1">MLSDATVQRVDLQGYSESIRWSDDGSLAVNTTGSLTILKPRYQSGALKSANELFEIEKLEIESWPRNGLYKNQPADEVVSTNFSSTDDFVTKLLWSPLTQNRQSFLAVLTASKSVNILENGKVKTMIGEYATFKTQKEMDDAVTYSFVWYKKDQDLYFITGQASGNLKFYKFDDDEFKFLQGISLPSSSPIVKLKVDSGVLVAVSADNEIIQVNDLLSEKDQEIKILKSKDRFSVYDIFLTQEFVVFTSPAKFQKIDLKTGSIYEVITNIYDSSIIIPNGSSFILISNTTTVKVDQNLKVQADDKVHPVKIKRLKRWNSRIDSFRLKSNTIKIYGADYNFSGDVLAILYEIEDQSGFKYKISSENKYNIAFLSLDNKFDNVGSSLAIYQEFMLTGKIPPTTIENLSKPTPKGQDFKDYLKSNVLKDAEILKLIANNIISRDKDAEIRARYSQLLLEFVKQNEVPLENSLDRVTYNYIVNAVNGNSNEESQEFKVVTDILEETFKTDKNNNPDALVSESGHAWNRCALTFLPIVSTNVKVDPVTQKRIIDISKDELNEYGYFTRTILETLNKVSIYSGCRFGSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P720</accession>
    <name>A0A1E3P720_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_16500</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040438.1">
        <property type="nucleotide sequence ID" value="XM_019180694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P720"/>
    <dbReference type="STRING" id="683960.A0A1E3P720"/>
    <dbReference type="GeneID" id="30197940"/>
    <dbReference type="OrthoDB" id="5424147at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024461">
        <property type="entry name" value="CCDC90-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360:SF12">
        <property type="entry name" value="MOZ PROTEIN REPRESENTS A CHROMATIN-ASSOCIATED ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07798">
        <property type="entry name" value="CCDC90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="169" mass="19676" checksum="041D659F13299A86" modified="2017-01-18" version="1" fragment="single">YVDTMHIYNQLKKLGFNNGQSDLILQLINENLTHQLNKMNEKFTTSMEMENESYLFEAAQSELRIEINTSRELDLHTLENEKNSLDLLLREESEELNKFMIVSKNDTQVLINDQNSENTLMQKGIKMKIKDLDNKISTNINSDIKSDIESLRWQTTRSGLFAILVLVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7I9</accession>
    <name>A0A1E3P7I9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_104465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ61386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3P7I9"/>
    <dbReference type="GeneID" id="30197663"/>
    <dbReference type="OrthoDB" id="2567806at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031349">
        <property type="entry name" value="Tfb6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37781:SF1">
        <property type="entry name" value="ADR380WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37781">
        <property type="entry name" value="TFIIH COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17110">
        <property type="entry name" value="TFB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="273" mass="30888" checksum="715BEF038F6D1394" modified="2017-01-18" version="1">MSDQANYSDAPNSPVHPDPDQEASNLPERSLPGSVLDLLDLNPDLEAKTATESTTRNTNGLEHTIDDHLYDRLEDFQPTLVPSPLNPVQPHLGRRLSSSQQSKFINYADERLLHIQRRFVQSFGLSELGYSSIDELLNDVKQLLNFVWLSIDSNPTIHDKEYYSTLPDTNFGQTHFLIRIAGDLVEYVGKMEITQASGVEILKLLKSLDDKFARLIDGTIPGGKSINRTESVRLYGIAERTRVLVSDLFEKKQIEGFHYELSKVYEQVLDRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7U3</accession>
    <name>A0A1E3P7U3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_52671</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040696.1">
        <property type="nucleotide sequence ID" value="XM_019182695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7U3"/>
    <dbReference type="STRING" id="683960.A0A1E3P7U3"/>
    <dbReference type="GeneID" id="30199941"/>
    <dbReference type="OrthoDB" id="1405595at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000065">
        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF11">
        <property type="entry name" value="ZINC FINGER PROTEIN YPR022C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="42"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="589" mass="67920" checksum="380059A32B2F6D4D" modified="2017-01-18" version="1" fragment="single">MVHLDTGSITKSRPFECPTCQRRFTRIENLRRHERQHSQQRLSCQHCLKLFSRNDALKLHLKRFHQNGSILKSAASVKIDSPLEKVSRDKLETLLQLYWENFHPTFPILHKMTFRSPGDYDDDDDDDDATIIHEFAQNALVNIMCCIGLRYLKDDESKQLRLSLEAYVLKKLQTPSVEDPALTDLQASLLYIHCGIYSGNEEWFKKAEIELHYLIEIARETGIFQNTPAVSQLGKEWDKFIFSESRRRISYAIYFIDSQLATLFNYPPVTSHYEIKHILPCSDELWEMPTAEDWEAYKESHVETQGLYFLEGLQDALIYGKMSKPTSSFGAMTILSAMHIMIRHMTQYAGILETVPSHAQDPFSRRSQLGNGLNGLRTLIPKNTHSDPFRQKTMWDLFEYFWNLAYIHLHLPDTVITSGIIEVSLDATVVTCATLAKPQSKNPPGIALLNNDFSQIPYQTHALASSHLGFFLSSYKKGVQEQNPAMTFMFYKACLKAWQILNVALKKDEAENFEYPLELDEQKRQMLESKYLMQRLAGDILCNLEGSTDYSDLQYFENWVESSLSSLNTWGIGDCASCSFRDMLIEPQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7W6</accession>
    <name>A0A1E3P7W6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription activator GCR1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_61528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040174.1">
        <property type="nucleotide sequence ID" value="XM_019183193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7W6"/>
    <dbReference type="STRING" id="683960.A0A1E3P7W6"/>
    <dbReference type="GeneID" id="30200439"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060963">
        <property type="term" value="P:positive regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052146">
        <property type="entry name" value="HOT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022210">
        <property type="entry name" value="TF_GCR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784:SF2">
        <property type="entry name" value="HIGH-OSMOLARITY-INDUCED TRANSCRIPTION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784">
        <property type="entry name" value="PROTEIN MSN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12550">
        <property type="entry name" value="GCR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription activator GCR1-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="118" mass="13996" checksum="6F3D62C0D9CAB9AB" modified="2017-01-18" version="1">MKTISINSIQNPKSVQEVLDEYYYGYQGQPSLKYLEESQKRWRKGNKNLSKTFSRRFRVVTAVEIGTQMYAAEMGGNEALAKERVVNELENLRNRENGGRETMYWLFHHIPEHLKRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P859</accession>
    <name>A0A1E3P859_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flavodoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_26357</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WrbA family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040802.1">
        <property type="nucleotide sequence ID" value="XM_019181249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P859"/>
    <dbReference type="STRING" id="683960.A0A1E3P859"/>
    <dbReference type="GeneID" id="30198495"/>
    <dbReference type="OrthoDB" id="504689at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032126">
        <property type="term" value="C:eisosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160020">
        <property type="term" value="P:positive regulation of ferroptosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000001">
        <property type="entry name" value="NAD(P)H dehydrogenase (Quinone) FQR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010089">
        <property type="entry name" value="Flavoprotein_WrbA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01755">
        <property type="entry name" value="flav_wrbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002999">
        <property type="entry name" value="PRK03767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546">
        <property type="entry name" value="FLAVODOXIN-RELATED PROTEIN WRBA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546:SF23">
        <property type="entry name" value="FLAVOPROTEIN-LIKE PROTEIN YCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="2">
        <location>
            <begin position="5"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="200" mass="21237" checksum="DE758E29E492ED17" modified="2017-01-18" version="1">MAPKVAIIIYTLYGHVAKLAEAEKAGVEKAGGQADIYQVPETLSDDVLKLLHAGAKPNYPIATPETLEQYDAFLFGIPTRYGNFPAQWKAFWDSTGGLWAKGALYGKVAGVFVSTGTPGGGQEVTIVNSLSTLAHHGIIYVPLGYKNAFPLLTNLEEVHGGSPWGAGSFAGGDGSRTASKLELEVAEIQGSSFYETILKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8G3</accession>
    <name>A0A1E3P8G3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_86630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040910.1">
        <property type="nucleotide sequence ID" value="XM_019186060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8G3"/>
    <dbReference type="STRING" id="683960.A0A1E3P8G3"/>
    <dbReference type="GeneID" id="30203306"/>
    <dbReference type="OrthoDB" id="3980190at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019540">
        <property type="entry name" value="PtdIno-glycan_biosynth_class_S"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10510">
        <property type="entry name" value="PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133758" evidence="1">
        <location>
            <begin position="19"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="298" mass="34802" checksum="FDC3884175F0A525" modified="2017-01-18" version="1" fragment="single">MLYFNLFLIIGVLLRVSAAEFNLTIPIYFDIPKHLSFLVPKTQQSLDTKLAHYDFKINYKTHCLLGKGPSYTVKLDYAKDESYFISPYGKTIIVYQTDLLIAHNKIPEFIAQLIVEFVLKYEVEYFDKKTINDADNVGIELAPSYDVKILIKAPVDFQWNYELFQYLFQPVLNELQDLIYLNVIFDYVYEEDNTVLHLEDIESILDYEKLTILVHLSSKEQKISGIDSNNIIIPKKGAVQLARVEETLSEMQQIEILEVLTGQLFRLLGMEPTEPKSPFIRIDFMIRKQVYINYQKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8I0</accession>
    <name>A0A1E3P8I0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_62079</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040731.1">
        <property type="nucleotide sequence ID" value="XM_019183306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8I0"/>
    <dbReference type="STRING" id="683960.A0A1E3P8I0"/>
    <dbReference type="GeneID" id="30200552"/>
    <dbReference type="OrthoDB" id="10266058at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12232">
        <property type="entry name" value="RRM3_U2AF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000097">
        <property type="entry name" value="U2 snRNP auxiliary factor large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23139">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="395"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="499"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="44"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="125"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="603" mass="69688" checksum="A97C3C3CED782425" modified="2017-01-18" version="1">MSTHNVLEALKQKLLREQAQNESIRPSETPVKREVTSSNSGYGRRPDDTKNTPDRSGYSRREERANEVKQESPQEQKPIETRSMDDRQTHDRRDYRGTRGGDHYSSGRREYPNRNDHHDRRHTAERRDYGERRDYSGRDRRDYNRRDDGPSRYRDDRYERRDERPTRYRERDTRGGDRYYDNTNNQQQRYSRSTDYSNNGDDASYNEDLTNVVPIDKLERKSLWDLKPKGFEKVSSERAKISGLFPLPGESKQVDISKLEGLVSGGDLNSQTSILFEEISIESLSSRNSKQLIITGVEFSLFPVERLVEYLKTYINSLKVENAFIVSHELIESKYLVINLSNSNITTILYSSFKYFQNELHLKIKIDRPQSYITPNSKLLSPEELSKSKVPDSGNKIAITNIPNDIKQDEILQDLQEIGHVSKIDLLLDNEENSKGIAFVEFEDIIADQAIEAINKIVINETTLFAIFASKGLPKQEEEITYRTISSVAQSGSLKIDTNVLILLNVVHPEELKNEVVYQEVLNDITNECSKYGKVLNVKIPKPAEDYKRNLKSLDTSIGKIFVKFENEEGASRASKLLNGRKYHERTVLVSFLPESDYNLNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8L2</accession>
    <name>A0A1E3P8L2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Domain of unknown function at the cortex 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_13266</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040948.1">
        <property type="nucleotide sequence ID" value="XM_019180565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8L2"/>
    <dbReference type="STRING" id="683960.A0A1E3P8L2"/>
    <dbReference type="GeneID" id="30197811"/>
    <dbReference type="OrthoDB" id="2119945at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013897">
        <property type="entry name" value="Duc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34826">
        <property type="entry name" value="UPF0590 PROTEIN C409.17C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34826:SF2">
        <property type="entry name" value="UPF0590 PROTEIN C409.17C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08588">
        <property type="entry name" value="Duc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Domain of unknown function at the cortex 1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="268" mass="30231" checksum="760494590DA7027C" modified="2017-01-18" version="1" fragment="single">LKISASTAYDGEFKLVPVNSEKPLSFHSEIGDIQLLLRVKDFYGSKDHDKSSSKDSEYFKSNPNSNISIQISFTPNQDGITGDKLLFGNDFEYPIKDYLPYGTSTGLKLFKNYVDPSVDGDLFAEKPYLYGKALSSFNVINDKLKPNSNEGENHFIKEDLVNDEPTSDKLLIPVESTDRQKFFQDIKNLEGFEFKKGHTYTFDFSSGLVSLKNSNFNIKLPGGFEVNLSNYLNEKFNSVRYVLKKGTSAELGVEFGEPILIINFELVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8N1</accession>
    <name>A0A1E3P8N1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Anaphase-promoting complex subunit 4 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_102611</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat MORG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040935.1">
        <property type="nucleotide sequence ID" value="XM_019180361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8N1"/>
    <dbReference type="STRING" id="683960.A0A1E3P8N1"/>
    <dbReference type="GeneID" id="30197607"/>
    <dbReference type="OrthoDB" id="1068471at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051980">
        <property type="entry name" value="WD_repeat_MORG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22842:SF3">
        <property type="entry name" value="WD REPEAT DOMAIN-CONTAINING PROTEIN 83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22842">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="32451" checksum="F4307ACB78735190" modified="2017-01-18" version="1">MKFHYDSHFHTESHINSIKFNTEQYLFYTQKNTIQLTSPSINTQLVYSGHNNSINSIDILDDQLLSCSSNIQIHNLLQNEAPIRKFYKDDPFKINQVIFLNNSLISSVDDNKSLKIFDLKQSTTFKPIQTYSNPKDSINCVCYDDDGFLLICGSNDGNLYTYDLRMGLLYIDQFQTPITSIKARNGGVLINCLNQGLIKFDIKSKQKVEFGGIDRSNDEDYKYDNAILEIDDGYCYVTGSRHKLYGFGGLNDVVEMKGSVLSSIDHCDGCLVIGSDYGVEKVRVND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8N7</accession>
    <name>A0A1E3P8N7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_75876</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040877.1">
        <property type="nucleotide sequence ID" value="XM_019184279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8N7"/>
    <dbReference type="GeneID" id="30201525"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="248" mass="28179" checksum="5F936E2007282D5D" modified="2017-01-18" version="1">MKRIMVNQIDGADNVDYIPLNEFKSLAYYEALFLKDPQLAPVIQENICIIADNLGDGESNWDDVPSDILRDIPFGRMDLETFDSSQTTADYFIIFVHYVGSSELANLIKTLVENKKFHQLNIYNNETFYEFDKHFKDIPNDPYLEFLNYDEQDTYLIEVGTEQCLFFAGDSPNFDYLMGTHEGVLKKACSSPTCLNLIIEIKSKTPRNLIFKCQKFPKLVNTTVNCNGNSSSITFEGCNIGPSGLPIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P917</accession>
    <name>A0A1E3P917_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">diacetyl reductase [(S)-acetoin forming]</fullName>
            <ecNumber evidence="2">1.1.1.304</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_49048</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(S)-acetoin + NAD(+) = diacetyl + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27286"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15687"/>
            <dbReference type="ChEBI" id="CHEBI:16583"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.304"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.304" evidence="2"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041116.1">
        <property type="nucleotide sequence ID" value="XM_019182626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P917"/>
    <dbReference type="SMR" id="A0A1E3P917"/>
    <dbReference type="STRING" id="683960.A0A1E3P917"/>
    <dbReference type="GeneID" id="30199872"/>
    <dbReference type="OrthoDB" id="47007at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052588">
        <property type="term" value="F:diacetyl reductase ((S)-acetoin forming) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045150">
        <property type="term" value="P:acetoin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014007">
        <property type="entry name" value="23BDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02415">
        <property type="entry name" value="23BDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF121">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="61"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="184"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR614007-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR614007-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="258" mass="27463" checksum="066A0C0D5808AA42" modified="2017-01-18" version="1">MSKVALITGAAQGIGKSIALQLAKDGYKVAILDLPTQEQLGQEVVSSINKIGQDSLFIPGDVCSRSEIFNAVDKTFNKFGGFNTMINNAGIAKLGPLLSITPESWEKSLQTNISSTLWGIQASAKKFIELNQPGKIINAGSVASHEGFVNLGIYSVTKHGVKSLTQTAAKELASQKITVNSYCPGMIPTNMWDSINEMTGDYSGRAKDALLKSYTPKIAMGKVGDAQDVADLVSFLASEKSNYITGQSYVVDGGLVFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P950</accession>
    <name>A0A1E3P950_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_86975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041143.1">
        <property type="nucleotide sequence ID" value="XM_019186083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P950"/>
    <dbReference type="GeneID" id="30203329"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="143" mass="15839" checksum="02A8D3DF3B4CFF81" modified="2017-01-18" version="1">MKRAREEYEEELGESEEELKVPEDSDEEEDGFGSYVPHIHYSGFAQFVPALPTNEDIDARLSGINLRGSDNPIPEDSNRGGTGNRGRGHRGRGRGHRGGWRGRGRGRGRNNGGNTLRGDVTPPPRFNNSRPSRTWSDALVSPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9G2</accession>
    <name>A0A1E3P9G2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA polymerase</fullName>
            <ecNumber evidence="12">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_82096</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="12"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041147.1">
        <property type="nucleotide sequence ID" value="XM_019185334.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P9G2"/>
    <dbReference type="GeneID" id="30202580"/>
    <dbReference type="OrthoDB" id="6755010at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000510">
        <property type="term" value="F:H3-H4 histone complex chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000731">
        <property type="term" value="P:DNA synthesis involved in DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006278">
        <property type="term" value="P:RNA-templated DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05776">
        <property type="entry name" value="DNA_polB_alpha_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05532">
        <property type="entry name" value="POLBc_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000004">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000003">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000036">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2820:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3200.20">
        <property type="entry name" value="DNA Polymerase alpha, zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024647">
        <property type="entry name" value="DNA_pol_a_cat_su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038256">
        <property type="entry name" value="Pol_alpha_znc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045846">
        <property type="entry name" value="POLBc_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015088">
        <property type="entry name" value="Znf_DNA-dir_DNA_pol_B_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45861">
        <property type="entry name" value="DNA POLYMERASE ALPHA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45861:SF1">
        <property type="entry name" value="DNA POLYMERASE ALPHA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12254">
        <property type="entry name" value="DNA_pol_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08996">
        <property type="entry name" value="zf-DNA_Pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 12" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase alpha catalytic subunit N-terminal" evidence="17">
        <location>
            <begin position="7"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="15">
        <location>
            <begin position="383"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="14">
        <location>
            <begin position="697"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger DNA-directed DNA polymerase family B alpha" evidence="16">
        <location>
            <begin position="1182"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="58"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="206"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="730"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="218"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="733"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ61940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1379" mass="156184" checksum="4A9DC754A7270496" modified="2017-01-18" version="1">MAKADKLKALRAARAAGTSKNVYHEEEPEDIYDVVDEDDYRAKRREDLLKDDFVIDDNGEGYVDTGADEWDNRGRHRYHSSEDEEEVEDSHSRKKKKVKKTAPINNFFKPSSTVAAATKKKVDANFDDILDDFMVNETSFDNHMDSSPTRTKQAKAESAELEIPVIAKKPEAIKGEQEDEDEESEDEVVVVKRPRTSTTNVNRSVNMTSSAKQESIKSDPVTFTSSPSSSYTPTSDKIDKELVADDSTDSFKMYWLDYTEVENTLILFGKVLTNDKQLVSAMVQVKGFDRELYFLPRDYRQVDGEDTDEKISAMDVHEEVAPLIMGKFGLSSLKAKPEVKKYAFEMPGIPKEKEYLKLILPYQPPKSAKVTLPSDLSGETFSHVFGTNANIFETFVLQRNIMGPCWLEIKNADFNAVRNTSHCKVEVAVGKPAFITPIEKSTPEFQITPPPLTSCSISVQSMMNQKENRQEVVAVTLATYRNLPQDAPVDENLKPDDFVTLTRPIGVSFPPGLKTLADKNKLPLRVFSNEKTLLNCLAALVKLSDPDVFIGHRLENISLDIVLHRMHDLKVATFSTFGRRQRKAWPDRFGKNNANTSFLIKEIFAGRLLCDISNEMGQSLTTKCQSWDLPEMYDVVCKKKHMPLEINWANPQYAENANSLFMVLKENCMNCLITAEIAFGMQILSLSKQLTNLAGNAWAHTLGGTRAGRNEYILLHEFNRNGYIVPDKETRQQRQAFQQQQQQDMNDDGDEAQQQSSGNNKKSKYQGGLVFEPEKGLHKNYVLVMDFNSLYPSIIQEFNICFTTVDRDLNNIENLPDVPANSTQQGVLPRLLSTLVNRRREVKKLMKDPTASAVEKAQYDIKQQALKLTANSMYGCLGYVHSRFYAKPLAMLVTNKGREILMDTRQLAESLGLRVVYGDTDSVMIDTNCDNFKEAIKIGEDFKVKVNERYRLLEIDIDNVFKRLLLHAKKKYAAMNVSFDKMGKEIATLEVKGLDMKRREYCPLSKDISIYVLQKILSDLDPEVALNEVYSYLEETTSKIKNFEIRPENFKINTKLSKDPSAYPGGKNMPAVQVALRLKEQGKVIKAGSVITFVITEGDEGSVAERARSLNEMLAKNSTLKPDPNYYLEKQIFAPVERLVERIEGVDIVRLAKALGLEHRSKQLNNNSNNGASMGTLQPLESTIPDSERFKNAAHLFLNCKCGNHFKFGGIVASHDYQITFNGVQCKKCESQISTLGVSSQLEHRIRSFIAKYYQGVVVCDDSTCGMTTKQISVYGKRCLSHGCKGVMRYKYSDKELYNQLLYFDSLFDVSKNKTQSLRPLYDSEDKSQPTPMIKGQIDALSEQNRENFEVFKSVVQKYLSDCGRRYVDMGSMFDFMNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9M1</accession>
    <name>A0A1E3P9M1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ketoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_60179</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041322.1">
        <property type="nucleotide sequence ID" value="XM_019182952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9M1"/>
    <dbReference type="STRING" id="683960.A0A1E3P9M1"/>
    <dbReference type="GeneID" id="30200198"/>
    <dbReference type="OrthoDB" id="153074at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05367">
        <property type="entry name" value="SPR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000281">
        <property type="entry name" value="Uncharacterized oxidoreductase YIR035C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF8">
        <property type="entry name" value="BENZIL REDUCTASE ((S)-BENZOIN FORMING) IRC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="5">
        <location>
            <begin position="2"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="252" mass="27250" checksum="D8D8776002CA91F8" modified="2017-01-18" version="1">MGVIILTGASRGIGAVMADILLKNKENNLVAVARSEGPLRNLKIKYGEERVATVVGDLSEASVAKKIIDTAITQFGRIDSIIANAGVLQPVAQIKDVDIDQWKKLFDVNFFSIVSLVSLALPELRKTKGHVVLVSSGASTKSYDGWGAYGASKAAINHFAIQLAGEEPDVSIVSVAPGVVDTQMQVDIREKFGQNMTPEGLKRFTDLKSNNELLDPSVPGTIFANLAQRGFSKEINGKYLRYNDPLLESYTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA61</accession>
    <name>A0A1E3PA61_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_24815</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041488.1">
        <property type="nucleotide sequence ID" value="XM_019181059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA61"/>
    <dbReference type="STRING" id="683960.A0A1E3PA61"/>
    <dbReference type="GeneID" id="30198305"/>
    <dbReference type="OrthoDB" id="411372at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16464">
        <property type="entry name" value="RING-H2_Pirh2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039512">
        <property type="entry name" value="RCHY1_zinc-ribbon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008913">
        <property type="entry name" value="Znf_CHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037274">
        <property type="entry name" value="Znf_CHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017921">
        <property type="entry name" value="Znf_CTCHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037275">
        <property type="entry name" value="Znf_CTCHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319:SF0">
        <property type="entry name" value="AND RING FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G08900)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319">
        <property type="entry name" value="RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05495">
        <property type="entry name" value="zf-CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14599">
        <property type="entry name" value="zinc_ribbon_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161219">
        <property type="entry name" value="CHY zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161245">
        <property type="entry name" value="Zinc hairpin stack"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51266">
        <property type="entry name" value="ZF_CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51270">
        <property type="entry name" value="ZF_CTCHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHY-type" evidence="6">
        <location>
            <begin position="121"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="CTCHY-type" evidence="7">
        <location>
            <begin position="191"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="258"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="44294" checksum="1E85B3ADDC3CD043" modified="2017-01-18" version="1" fragment="single">MLDQVIIPESKEPYRNRHHKKKHKHITREIATNSELEELAYVSQSELNDEAFLRKRIKKIRRLSISEEEKNRLTQKLMMGAHYDKIRFQETQEDENMTTEDEDDDLVIVNDCDKVPTYHNESLEIFGCPHYQRNCKMECPRCKQWFGCPICHDEVVTDHKFKREDTRHVMCLKCFEVQEPSEFCDNCDIQFALYYCSKCKLYDNDENKDIYHCDDCGICRLGLGLGQDFFHCKGCNACLSIELQDDHRCIERATQSDCPICGEYMFTSVKPVVFMSCGHAIHQSCYEDHSKHSYKCPTCQKTVLNMEAQFRVLDMEISLQPLPQPYCQWTCVVSCNDCNAKSSCAYHVLGLKCDNCKSYNTVQLKLIKPEESDYESDIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA86</accession>
    <name>A0A1E3PA86_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome production factor 2 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ribosome biogenesis protein RPF2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_98832</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
            <location evidence="1 4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the RPF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041536.1">
        <property type="nucleotide sequence ID" value="XM_019186670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA86"/>
    <dbReference type="STRING" id="683960.A0A1E3PA86"/>
    <dbReference type="GeneID" id="30203916"/>
    <dbReference type="OrthoDB" id="407658at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
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    </evidence>
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    </evidence>
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    <sequence length="316" mass="36257" checksum="F416F06DA976FD6C" modified="2017-01-18" version="1">MIRTVKPKNARSKRALKNKEAKLVENTKQALFVPGSTSNKVLHDTMVDLSALKKPDAKRFNKKNDGRPFEDSSKFEFFSEKNDTSLLVYSSSSKKRKNNLTFIRTFGYKVYDMIELLISDKYKLLSDFRKTTFAIGLKPMFTFNGAVFDSHPVYKHIKSLFLDFFRGQVTDLQDVAGLQHIISITAADFQEGEPLPQVNFRVYLLKTFKSSQKIPRVELSEIGPRLDFAVGRYEHPSPDMEKEALKKPKELEAKVKKNVETDSMGDKLGRIHVGKQDLGKLQTRKMKGLKKRYDQVDEDFDDPVYDDEPSAKKAKI</sequence>
</entry>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
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                <person name="Barry K.W."/>
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                <person name="Hanson S.J."/>
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                <person name="LaButti K.M."/>
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                <person name="Meier-Kolthoff J.P."/>
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                <person name="Otillar R.P."/>
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                <person name="Kurtzman C.P."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
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            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
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        </location>
    </feature>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <sequence length="106" mass="11733" checksum="729322A24A9E51C4" modified="2017-01-18" version="1">MNSSAPFKQFLNNFWFVSSLAIGAIYVTKTIIENKREKSFHPEHFDNGDNKVSVQDNIAKIKPGFAESTGEYKRKSEYEGAGNSYSSRKGGDKFSGILDFGGAPKT</sequence>
</entry>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
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                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
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    </dbReference>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="228"/>
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        </location>
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    </evidence>
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            <dbReference type="EMBL" id="ODQ62395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
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    </evidence>
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</entry>
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        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
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            <taxon>Eukaryota</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
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                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="30203384"/>
    <dbReference type="OrthoDB" id="3981312at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="110" mass="13152" checksum="82588424941C9A8E" modified="2017-01-18" version="1">MKTTRQVLDDLKRQRTILMKKHAEAMKSKKNDEKRLSNVRELQANLMRKTRQIKNLNGNSRPKSYVYGEDPTRILQQRQQSDSENYFDYQNNQSYNFETFMRSKSSTALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAW1</accession>
    <name>A0A1E3PAW1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Autophagy-related protein</fullName>
        </recommendedName>
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        <name evidence="12" type="ORF">WICANDRAFT_26512</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
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        <lineage>
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            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Autophagosome membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Vacuole membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ATG8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041762.1">
        <property type="nucleotide sequence ID" value="XM_019181272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAW1"/>
    <dbReference type="STRING" id="683960.A0A1E3PAW1"/>
    <dbReference type="GeneID" id="30198518"/>
    <dbReference type="OrthoDB" id="6738456at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120095">
        <property type="term" value="C:vacuole-isolation membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008429">
        <property type="term" value="F:phosphatidylethanolamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140042">
        <property type="term" value="P:lipid droplet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061025">
        <property type="term" value="P:membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071211">
        <property type="term" value="P:protein targeting to vacuole involved in autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031503">
        <property type="term" value="P:protein-containing complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016241">
        <property type="term" value="P:regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905153">
        <property type="term" value="P:regulation of membrane invagination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16128">
        <property type="entry name" value="Ubl_ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000149">
        <property type="entry name" value="microtubule-associated proteins 1A/1B light chain 3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="lipid moiety-binding region" description="Phosphatidylserine amidated glycine; alternate" evidence="10">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR604241-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="133" mass="15478" checksum="8E5EFE826C392E5C" modified="2017-01-18" version="1">MFFQFKEEHSFEQRKAEATKIRQRFNNRIPIICERAEKSNLPEIDKKKYLVPYDLTIGQFVYVIRKRIHLPAEKAIFIFVNDILPPTAASMSSIYEEHKDEDGFLYVLYSSENTFGDATVDIASLPGVIELNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAX3</accession>
    <name>A0A1E3PAX3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_50164</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFA1 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041748.1">
        <property type="nucleotide sequence ID" value="XM_019182646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAX3"/>
    <dbReference type="STRING" id="683960.A0A1E3PAX3"/>
    <dbReference type="GeneID" id="30199892"/>
    <dbReference type="OrthoDB" id="1920692at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017384">
        <property type="entry name" value="NADH_Ub_cplx-1_asu_su-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17098:SF2">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17098">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE MWFE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15879">
        <property type="entry name" value="MWFE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ62541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="87" mass="10393" checksum="C712E3069C47D0D0" modified="2017-01-18" version="1">MIPFEGLLPYGIMMTLLTLGGGLVTATRVYQNNGHRVRYAYDNWEEQMMERDYRLTGKFREQVANEKAPEVFKTNSFWKLEKPSRWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB32</accession>
    <name>A0A1E3PB32_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_76331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041359.1">
        <property type="nucleotide sequence ID" value="XM_019184366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB32"/>
    <dbReference type="GeneID" id="30201612"/>
    <dbReference type="OrthoDB" id="10664520at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="196"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="266" mass="29892" checksum="0C1FD4A65B454977" modified="2017-01-18" version="1">MSKTETAMKQTKYIKFSYRSPTKRKGIKQENDGMESIPRIITSQEDEHSPTRVKREREEDEDEGTNTLQQQAKEGYVRVDDDYYSKLDKNQYPFSSKYIPMKPASEDEGSQSPILNNPSILLHQMDNYKTVSAESEQQWGQVSHQVVDSLRVWRKLYKILDNKKRQLKKAQCKKNSSSTGFKNGGVDGGLNGGGLSNNSSNDSSNGNEESEDQVSSDNSNGRATKRRKLSGGQQQGEDTQQLVGGEALGNGYSSNERKTRSTRSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB64</accession>
    <name>A0A1E3PB64_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein ECM19</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">WICANDRAFT_60714</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041863.1">
        <property type="nucleotide sequence ID" value="XM_019183040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB64"/>
    <dbReference type="GeneID" id="30200286"/>
    <dbReference type="OrthoDB" id="4080273at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="89" mass="9937" checksum="E9E663D776E868FD" modified="2017-01-18" version="1">MSRINFFNFSAIALVCTAGVYTGTKFFEPIVVERLRLDGNLRDDIEVPKYDENGELIVAEPQQLPPKPSEFLTDKQAASSNQEQSDASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBA2</accession>
    <name>A0A1E3PBA2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_26904</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit p55 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041449.1">
        <property type="nucleotide sequence ID" value="XM_019181314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBA2"/>
    <dbReference type="STRING" id="683960.A0A1E3PBA2"/>
    <dbReference type="GeneID" id="30198560"/>
    <dbReference type="OrthoDB" id="268763at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000070">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054559">
        <property type="entry name" value="PSMD12-CSN4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040134">
        <property type="entry name" value="PSMD12/CSN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040896">
        <property type="entry name" value="RPN5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12/COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22241">
        <property type="entry name" value="PSMD12-CSN4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18098">
        <property type="entry name" value="RPN5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="231"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="445" mass="51840" checksum="2459E1E28914499D" modified="2017-01-18" version="1">MSRDAPIKADKDYTQLLDEQFPQIDVLGKNDYNAALEKLLLLEKQTRQASDLASSKRVLTKIVDLLGDHKDWKALNEQLVLLSKKHGQLKLAVQVFIQEAMSYLDKTPDLTTKIEVIDTIRTVTENKIFVEVERARVSRELSHIRRSQGKIDEATEILVELQVETYGSMNLREKMEFILEQVELCILKGDFNQATILSRKILVKTLQLAEYQDIKLQYYELLIKIGLNKNDYLNIAKHFLSIYEIEDIKKDETKWKPVLINIVYFLILSPYDNLQNDLIHKLDLDTNLRKLDLHSKLVKSFITQELMRWPIIKDVYSGELQKSEFFNNKEKANEKHWSDLRDRVIEHNLRVISTYYTRITLLRLNQLLDLNETETEHFISTLVNQGTIYAKINRPAKIVSFAKPKDSNELLNEWSTNVDELLGHIETIGHLITKEEMMNGIKASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBK9</accession>
    <name>A0A1E3PBK9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">T-complex protein 1 subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">CCT-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_82711</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. In yeast may play a role in mitotic spindle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041968.1">
        <property type="nucleotide sequence ID" value="XM_019185429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBK9"/>
    <dbReference type="STRING" id="683960.A0A1E3PBK9"/>
    <dbReference type="GeneID" id="30202675"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03336">
        <property type="entry name" value="TCP1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000002">
        <property type="entry name" value="T-complex protein 1 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000045">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012716">
        <property type="entry name" value="Chap_CCT_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02341">
        <property type="entry name" value="chap_CCT_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="544" mass="58628" checksum="4D5378ECC2708138" modified="2017-01-18" version="1">MATVRSIKHLGGIGLIGQLEKCVQIYGDQVTEERAENARLSAFVGAIAVGDLVKSTLGPKGMDKLLQSASSDQALVTNDGATILKSIPLDNPAAKVLVNISKVQDDEVGDGTTSVTVLAAELLRESEKLIDQRIHPQTIIEGFRIATQAALSSLEKAAIDNSKDSDKFYEDLLSIARTTLSSKILSQDKEYFAKLAVDAILRLNGSTNLEHIQIIKKIGGKLSDSFLDEGFILNKRFGNSQPKKIENAKILIANTSLDTDKVKIFGAKFKVDSTSKLAELEKAEKEKMKNKINKITKFGVNCFINRQLIYDYPEQLFTDAGINSIEHADFEGVERLALVTGGEVASTFDNPELVTLGTCKSVEEVIIGEDVMTKFSGCAAGQACTIVLRGATEQVLDEADRSLHDALSVLSQTTKETRTVLGAGCSEMIMSKAVDTAAQNVDGKKSLAVEAFARALRQLPTILADNAGFDSSELITKLRSAIYNGMTTSGLDLDNGVITDVRELGIVESYKLKKAVVGSAAEAAEVLLRVDNIIRAKPRTANRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBN0</accession>
    <name>A0A1E3PBN0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Hpc2-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_60422</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041569.1">
        <property type="nucleotide sequence ID" value="XM_019182989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBN0"/>
    <dbReference type="STRING" id="683960.A0A1E3PBN0"/>
    <dbReference type="GeneID" id="30200235"/>
    <dbReference type="OrthoDB" id="5576775at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014840">
        <property type="entry name" value="HRD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08729">
        <property type="entry name" value="HUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hpc2-related" evidence="2">
        <location>
            <begin position="514"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="477"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="576"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="309"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="481"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="628" mass="68418" checksum="A1407128A2BD84E3" modified="2017-01-18" version="1">MAKKGISISSLLGPKDSKESDDDLVPISSSEMESSPKKKVVVHPETHEVITIDDSDDSGPELLTQDKFEADASNIIGTPSTLKKTDSGKIDIKSMLASNENTETEKPAPKKRKPRAAKKEAEPKPEPKKRAPRGSKKKAAEEAAAAKAKAAEEASIEEDKKGKEEESKDKPASKKKGKASTNATTTEDDKAAPPGNNSKEPSPKDQPVATTTTEPQNNEKVVEAPKDTKPTEKPIPILNEIAKKSSSFPETPAKEHSYVSPGPPVSSPYVLQSQNDPKLPKPNDIKSLINTENEHRIQLLPSPIPNNNPPTANNNPLTNTSAPITSNNTTTNTTPKPKPKAQKKPSITTDNKEVKPKGKPGPKKKDDKQETPAPLTFNQIQNQNLLKPDPKANQSLPSPEIVEVQKSPAQETTPQPEATKPTEPIIALHVPLKPPNAQNSPDTIEQVGATQVVFNVLKMAEDKYGWKNLHPEASKYALELMNDEDIDDDDDDEDDEKEEEEPEPQQRIDGRRLQKGKPKIGQYDKEDPFIDDSEMIWEEQRASTKDGFFVFYGPLVEEGKSAKIERADGTIKRNRKRVAGTSNVNAGNAKRRATNSKQTTPKLLVPIAPAAGNAVPANPTNVDIAPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBX4</accession>
    <name>A0A1E3PBX4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_26833</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the thioredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041584.1">
        <property type="nucleotide sequence ID" value="XM_019181305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBX4"/>
    <dbReference type="STRING" id="683960.A0A1E3PBX4"/>
    <dbReference type="GeneID" id="30198551"/>
    <dbReference type="OrthoDB" id="10263751at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000104">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46115">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="5 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="10">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="site" description="Deprotonates C-terminal active site Cys" evidence="8">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="8">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="8">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="9">
        <location>
            <begin position="30"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="103" mass="11277" checksum="62E9AEFBE00C5B1C" modified="2017-01-18" version="1">MVTVITSAEQFKEAIAQDKLVVVDFFATWCGPCKMIAPLLEKFSNEYSNAAFYKIDVDDLGAVAQEQEVSAMPTIIFYKNGAVVDKVIGANPNAIKQKITSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBY9</accession>
    <name>A0A1E3PBY9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_25325</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041941.1">
        <property type="nucleotide sequence ID" value="XM_019181129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBY9"/>
    <dbReference type="STRING" id="683960.A0A1E3PBY9"/>
    <dbReference type="GeneID" id="30198375"/>
    <dbReference type="OrthoDB" id="1928777at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14077">
        <property type="entry name" value="STKc_Kin1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001772">
        <property type="entry name" value="KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02149">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50032">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="104"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="KA1" evidence="13">
        <location>
            <begin position="1072"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="61"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="496"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="596"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="673"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="731"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="794"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="917"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="550"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="685"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="800"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="930"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="941"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="997"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1015"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1063"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ62734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1121" mass="122848" checksum="BC01FE70EA694F4A" modified="2017-01-18" version="1">MSDQDPDYHINPNYQKSNLLPKVRPSTAGTPTSSGIKKPPTAVPATPRMLRNTSGHQILQAGTTPQRSSSTHRPSGYSTPSGPTSLNNDNTKQTQFHRRSIGEWDFMKTIGAGSMGKVKLARHRRTNEICAIKIIPRAARLYARNHANDPPPVDQKELEKRQKEYEKEIARDKRTIREASLGKILYHPFICRLFEVITMSNHYYMLFEYVSGGQMLDYIVSHGSLKERHARKFVRGIASALDYLHRNNVVHRDLKIENIMISNTGDIKIIDFGLSNFYSNDALLKTYCGSLYFAAPELLSAHPYIGPEVDVWSFGVVLYVLVCGKVPFDDQDVSVLHEKIKKGKVDYPDFLSNEVVSLLSRMLVVNPNKRAGLAEVMSHPWMTRGYEGPPTSFVPHRIPLTLPLDQATITEMANLEFGQNEDQIRNDITQVVSSKLYQDASENWYRNQRAEENVHGGQIDPTTGFHPLVSIYYLVEEMLKRKKAKQSIVDVPQAVKEQPKEITPDTAAGAPTQEKPKVKIETPSQPPVISFPEAAHTSPRATSPPGFPDRQQQLPSTSHATGNDDFLLTQQQKHPKSAAVTDEHSKGFNSILRKFSQRRAHSQRRPPPSHEPTPAVSSVEDPLYHSTGFNHNGNNGAVPMETTDNGVKYDPKHLMSPQQDALVRRVGSLKLTKKSPFDTPEKPTVTDNSLSAPTNKVNKSHNRTVSAYAGPAEALSSGDAKVAPGANASKLPATATKKFHPSARAKSVGHAARRHPSGFVPNEGSENSNALPPLPTDLNDDAFFDDVTLDDYSPTDKPSLVTSNTSSNQQGGARLGELANTELSETQILAEAARAPEGSMPSIEYPRSLFLKGFFSVQTTSTKPLPIIRANVISVLTKLGVKFTEVRGGFICVHTPSIEQPSQVPDVQITKPDDFAQEDQSLKSSKSSSGDSAANQNQETTRVHSLEHTFDQLSTPSSKGGHRRKFSIGGSILGSYRRKNGGSAPPIPPTPVAATRYDSSQMAASPSAGTSNQGASSSHTDYDSSASLDSLNAGEGGSDMLVSSRIEQNKARTPSTSGEKSQHQQQHASSQASKARTPLKFEIHIVKVPLVGLSGVHFKKVLGNTWMYKALAGQILSELNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC97</accession>
    <name>A0A1E3PC97_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_103700</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042205.1">
        <property type="nucleotide sequence ID" value="XM_019180389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC97"/>
    <dbReference type="STRING" id="683960.A0A1E3PC97"/>
    <dbReference type="GeneID" id="30197635"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000028">
        <property type="entry name" value="ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000578">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000145">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF118">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="162"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="344"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="13"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="83"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="786" mass="88520" checksum="075A955AD21DB36A" modified="2017-01-18" version="1">MSITNHNQKIHNKFRDSDELLESQESRGDIRSNEENKSKKRKRKRKSATIPAVNDDEKVALPKAGLQLGENSQGEDLRKKFKSNGSGSSGNAGFKIHTNSQKDQPSGKKKISSKKGRKCDDEEEYRDSRAQNVTRDSLNTRAKELLQIRKTLPVYKNKDDILKRLQKNRVTVLIGETGSGKSTQIPQFLLPLNKKGIAVTQPRRVAAINLATRVAEEAGSMLGEKVGYSVRFDNKSTHHTKLKYLTDGMLLRELMVQATLPQYSTIIIDEAHERTVLTDLLMGFLKELLAKREDLKLIIMSATLDAEKFSKFYDDAEILYVEGRMYKVDRMYLDRSVDDIVDSTIKAIIQVNNGELEGDILVFLPGQEEIDKAVNLLERVAPGLPREAPLIVPLPLYAALPPAEQAKVFIPMKARRRKVILATNIAETSITVPGVRYVIDSGLRKVKVWRHQLGLSTLLTAPVSKASATQRAGRAGREREGKAYRLYTEGDYLKLPNQTEPEISRSNVTSPVLMLKRAGVDDILNWSWLEHPGEDSLISALTQLYQLKALDDQGKITKLGTQMAILPLEPQLSAILLNAHETGCLTQVVDIVSCLSVDNLVMNPPSETRDEVNEKRLSLCLLGARHGDLIMLKELFDFYIQFDNSQERQQWCKELALSYRGFKNVLRVRNQIRQYMVSLTKEKYLFDEEEDETKPMDIPSVLKSFLVGFVSNTAIGMPDRSYRTTTSGQLISVHPSSMIFSKKSDAIMYTDYVYTTKGYARNVSTIDLTWLQEVGSHLLASKTKVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCB3</accession>
    <name>A0A1E3PCB3_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_103719</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042225.1">
        <property type="nucleotide sequence ID" value="XM_019180390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCB3"/>
    <dbReference type="STRING" id="683960.A0A1E3PCB3"/>
    <dbReference type="GeneID" id="30197636"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133812" evidence="2">
        <location>
            <begin position="18"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="128"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ63018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="311" mass="32222" checksum="F191E6F0CFBF9150" modified="2017-01-18" version="1">MKASLFFGLIFLTACNATSVTTKTPLATSESKEGLSRSPIFDTKLVGKRTDDLAVPLFGDYETSDNVYKQFEEEAYEDEDEYLKGKTFGRLAKRLFGFGSSGRGSSSSSQSGGRSGGSSWFGGGSRGSSGSRGSSSSGSSGSRGSRGSSGSSGSSGSRSSGKGGNSGSGGSRSGGSRGSGSGSNRLSGNNGGSRGSNGGYGPGYGSNGGYGPDYSGRRGSGWRNPALAGAAAGLGYYYIWHHDRYGHSHIVRTETRYLAENVTFTSAIFENGGVATSFRALNVAKTDDYNYWKKLMPSFIVCIATILISCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCW0</accession>
    <name>A0A1E3PCW0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Succinate-semialdehyde dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="9">1.2.1.16</ecNumber>
            <ecNumber evidence="3">1.2.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">NAD(+)-dependent succinic semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_27138</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>succinate semialdehyde + NAD(+) + H2O = succinate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13217"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>succinate semialdehyde + NADP(+) + H2O = succinate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13213"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; 4-aminobutanoate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.16" evidence="9"/>
    <dbReference type="EC" id="1.2.1.24" evidence="3"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041924.1">
        <property type="nucleotide sequence ID" value="XM_019181342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCW0"/>
    <dbReference type="STRING" id="683960.A0A1E3PCW0"/>
    <dbReference type="GeneID" id="30198588"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07103">
        <property type="entry name" value="ALDH_F5_SSADH_GabD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000004">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000005">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF5">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="12">
        <location>
            <begin position="22"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ62717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="492" mass="53510" checksum="53C2ADBFED26A6C1" modified="2017-01-18" version="1">MVSIQLQKQNLLQTKLFINGSWKEGVTGKSFAILDPATNEEITKVSKASEADYEEAIQSAFTSFQSFKKTSARERSKLLSDLYQSLIKNKEDLGKILCYENGKPLKESIGEIEYAASFFQWYSEEAPRIYGDVIPSSSASKRIITIRQPIGVVGIMTPWNFPAAMITRKLGCAIAAGCTAVIKPASETPLTALAIGKLCEEVGFPPGVVNILATDHEYTSKVGALLCEHPKIKKISFTGSTGVGKILQKQASSTLKKTSFELGGNAPFIVFEDADIDKAIEGLVACKFRQSGQTCVCANRIFVQENVFDEFLSRAIEKVKGFKLGKGYDTNDSSITHGPLINKKAFEKIKSLVDDAKSKGAKVEIGGEGSSLGDNFYKPTILTNLTPEMDIFTEEIFGPIAPIIKFETLEEVISLNNSLDVGLSGYFFTKDINRMFKLGEALEIGMIGINTGAISEAAVPFGGVKESGFGREGSKYGIEDYTTIKTMIIGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PDQ3</accession>
    <name>A0A1E3PDQ3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PH-response regulator protein palI/RIM9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_77154</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042204.1">
        <property type="nucleotide sequence ID" value="XM_019184511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PDQ3"/>
    <dbReference type="STRING" id="683960.A0A1E3PDQ3"/>
    <dbReference type="GeneID" id="30201757"/>
    <dbReference type="OrthoDB" id="2354757at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035838">
        <property type="term" value="C:growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051380">
        <property type="entry name" value="pH-response_reg_palI/RIM9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28013">
        <property type="entry name" value="PROTEIN DCV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28013:SF3">
        <property type="entry name" value="PROTEIN DCV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="213"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="350"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="405"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="558"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="632"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="696"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="417"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="570"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="697"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="755" mass="83372" checksum="11235E1F1EFCC615" modified="2017-01-18" version="1">MAPRIRPATTLVVVLSIAFAFQLIAILSVPVTKSISICTYDGYKFGVFGLCRGNSCSKVGIGYTGKELDDVDGFSLPSNARRSVSKLLVVHPISAGFSIILLFSSALLHWHGPSNSLRFLFFLLLWTIPSFLISLLSFLVDILLFVPHLDWGGWIVLAATVLIAVSGVLLCIMRRTVSSRRVLKRKDTFGNDYQLEPLNYGYGTVDGNSDVKFGKNSYEGKEGNENEEEREPFVHESNETFDNSMNYTRTHENLNDYPSTQLYDSMRGENNRSGQSNSTGYRSPENILAPLSTAYRGEFNFDRGNENPTQEYIPSAPYPSDIPNAGYDANELQQASEPWRAPYPNTNDDLSPHPEAYNANSVPYVGSKPSGPRPMPTPTTTGNEELPMLPSELAAVSFNNMDGDVLTEPYSNRDSAESLSAPPLPSNLESSAEAETGQSLPGYPFNERPDPLIQETKRLYESDPPNSFNKAEETGITREATYRTKIANAIDQLQGPETNDPAVYSNPSFINTNESLLASQSALNASQLPPKIKEDDNDDELYGPVAALSNANNEHKDRIVPQNSMDDDASSSYSSHISSTVNSPVDATFTRTHSLLTPNRGLPNKTQTTSSMAIDNIYDRYARDSTATLNNASAEGKEDGLSANDYPFPDPHEKRVTPAPSFSNISLVSSNFTSVSQRGINPKYFEKNPEEKLLYLQQQQQQQEDQSQSKHPLTVQTRPRPLQTAKNNTDILIASNPDFSVLPINKRNGKRKPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUH9</accession>
    <name>A0A1E3NUH9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Putative gamma-glutamylcyclotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_81569</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gamma-glutamylcyclotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035941.1">
        <property type="nucleotide sequence ID" value="XM_019185258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUH9"/>
    <dbReference type="GeneID" id="30202504"/>
    <dbReference type="OrthoDB" id="1044435at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045038">
        <property type="entry name" value="AIG2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009288">
        <property type="entry name" value="AIG2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31544">
        <property type="entry name" value="AIG2-LIKE PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31544:SF2">
        <property type="entry name" value="AIG2-LIKE PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06094">
        <property type="entry name" value="GGACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Gamma-glutamylcyclotransferase AIG2-like" evidence="4">
        <location>
            <begin position="12"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ56734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="146" mass="16803" checksum="B29C18179BBF9C5E" modified="2017-01-18" version="1">MVSDVEFSGKPLFVYGTLMSDKVFYKVLSVLEGDKVKKSQYKLLHRVGLKGYKRCQVRNELYPAAIKSIQDSIEGVLIYGLSSKDMKYLDDFEGDEYERVSISVDDSDGNSIEAEFYEWTAGEEYLNIGIDWELDAFKIDEFLKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUJ3</accession>
    <name>A0A1E3NUJ3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA polymerase II transcription factor B subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_36954</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the TFB2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036078.1">
        <property type="nucleotide sequence ID" value="XM_019182494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUJ3"/>
    <dbReference type="STRING" id="683960.A0A1E3NUJ3"/>
    <dbReference type="GeneID" id="30199740"/>
    <dbReference type="OrthoDB" id="364513at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2610:FF:000001">
        <property type="entry name" value="General transcription factor IIH subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040662">
        <property type="entry name" value="Tfb2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004598">
        <property type="entry name" value="TFIIH_p52/Tfb2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00625">
        <property type="entry name" value="tfb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13152:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13152">
        <property type="entry name" value="TFIIH, POLYPEPTIDE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03849">
        <property type="entry name" value="Tfb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18307">
        <property type="entry name" value="Tfb2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor Tfb2 C-terminal" evidence="10">
        <location>
            <begin position="413"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ56871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="487" mass="55340" checksum="2AC31E1007DD1E3F" modified="2017-01-18" version="1">MSSYLFKSNVNEYLEGLPESVQSRLYQSPATCLAIFRLLPSLSKFFITSMIFNEKPVVVRDFDRWVKPNGKKELVEALRSIKSLHLLKENNGIAILNPVFRKSFKNVLTGAEVGNSFGVLCDTDDAHKVGIEYLDQYAANKWETILHFMVGTELDQTPSPGVLSLLTHSGLMEGKTPKTMTITNEGFQFLLQDVNAQLWTLLLQYLRMAESLQMDPVDVLNFIFMLGSLELGKDYSLSALSETQVNMLEDLRDYGLIYQRKSSSRRFYPTRLATTLTSDTTTLRSASSAMNKVIENAKHNAYAPSDIENSGTIIIETNFKLYAYTNSPLQIAILNLFVHLKSRFSNLVTGQLTRESIRKALVSGITAEQIISYLESHAHPQLRRAAEEQLEKKIEYDSNTNEKLNILQPTIADQIKLWQLELDRIQAFDGFLFTDFASEQEYQVLSKYAEEIGVLLWNDSTKKKFFVTKEGNAQVIAFANRKLRKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUP5</accession>
    <name>A0A1E3NUP5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_65747</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="GeneID" id="30201225"/>
    <dbReference type="OrthoDB" id="3979317at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ56865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="150" mass="17510" checksum="BEB0B5853BD47ECB" modified="2017-01-18" version="1">MSEVELLKQRIEKLEKEIDDLKQVTIFSDIQPYIEENQDKFKQLKISSIGLSYLIKYQKGTILKDIFQRVAKDLLQDVEDIQLLDPKSGKVLTGEEDMSLFEHDGIFHLDFQYASANCGHNHHDHDHYDSDEDFTDDENFYSSCSCTHPH</sequence>
</entry>
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    <name>A0A1E3NUT6_WICAA</name>
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            <fullName evidence="1">Uncharacterized protein</fullName>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
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                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019186633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUT6"/>
    <dbReference type="GeneID" id="30203879"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ56898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="55" mass="6105" checksum="553510581A81454B" modified="2017-01-18" version="1">MGDFVTTILLKDRLIRLDSGRHGKIILINGYSSNSKSQFTQLVEDFQITLCAGCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUT9</accession>
    <name>A0A1E3NUT9_WICAA</name>
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        <recommendedName>
            <fullName evidence="7 8">Glucosamine 6-phosphate N-acetyltransferase</fullName>
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    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_81682</name>
    </gene>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
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    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>D-glucosamine 6-phosphate + acetyl-CoA = N-acetyl-D-glucosamine 6-phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="2.3.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the acetyltransferase family. GNA1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.4" evidence="3 8"/>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036061.1">
        <property type="nucleotide sequence ID" value="XM_019185283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUT9"/>
    <dbReference type="STRING" id="683960.A0A1E3NUT9"/>
    <dbReference type="GeneID" id="30202529"/>
    <dbReference type="OrthoDB" id="10039976at2759"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00529"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004059">
        <property type="term" value="F:aralkylamine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004343">
        <property type="term" value="F:glucosamine 6-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="Glucosamine 6-phosphate N-acetyltransferase"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
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    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039143">
        <property type="entry name" value="GNPNAT1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <begin position="8"/>
            <end position="155"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ56854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="155" mass="17133" checksum="B779373004231BF7" modified="2017-01-18" version="1">MSSLPEGYSIRRLKATDFARGVLNTLTALTTVGDVSQLQFENVVAKWDALTLMNGSYIYNPVVIINEKDEVVATGMLFIEEKLIHNAGTVGHIEDIAVRKDQQGKSLGKLLIKKLLEIGDKAGVYKIILDCDPKNVGFYEKCGLNEAGVEMQIRF</sequence>
</entry>
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    <accession>A0A1E3NV26</accession>
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    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036097.1">
        <property type="nucleotide sequence ID" value="XM_019183985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV26"/>
    <dbReference type="GeneID" id="30201231"/>
    <dbReference type="OrthoDB" id="3980702at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="117"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="402"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ56890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="425" mass="47061" checksum="4147975AB7FD87EB" modified="2017-01-18" version="1">MSSGVADDEQQQQLPNSTKTTSSAANDHDKDDQLNQLSSVNASMAKNGGGEADGDHQTSPDLSNEIEKSKSKNDNKLPAVCNNEESNIPTPNSVVDAKEDSAQSEDEGMTTKHNDDTGNNDPNDTNGANTPISKRKDSEVPEDTPKPKRSRKLQGAGVKKPLYSRYESLYIVKAFKTISDRHPNLVLIDLFKIVGILFNEEANRQGFKERTSDQLYQKYKRIQHGLNHGETEYLDFFKQNKYNPKEWVPDFTNAGSDEILKYIKFPTLDDAIKEVLGSSIANLKPPLPDLYDDEDDDNLSVSHQTPVLNINEESKGEAAEEELLKLAGYNDGGENGSGSSITKQLKEKIKTLETSISVQDKEIQLLKEGMHARDQEIAFLKNMIKEDLSYIRHKINEPAHQPSTTSPTGTSDPKALITPSSKPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV70</accession>
    <name>A0A1E3NV70_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carboxypeptidase</fullName>
            <ecNumber evidence="11">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_36180</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Release of a C-terminal amino acid with broad specificity.</text>
            <dbReference type="EC" id="3.4.16.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="11"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036308.1">
        <property type="nucleotide sequence ID" value="XM_019182405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV70"/>
    <dbReference type="STRING" id="683960.A0A1E3NV70"/>
    <dbReference type="GeneID" id="30199651"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046938">
        <property type="term" value="P:phytochelatin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.410:FF:000001">
        <property type="entry name" value="Carboxypeptidase Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF51">
        <property type="entry name" value="VACUOLAR SERINE-TYPE CARBOXYPEPTIDASE ATG42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ57101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="459" mass="51258" checksum="25A0831FB06D1746" modified="2017-01-18" version="1">MDFGKSQVPKALKQSLRLFDSSKFEFITNAKFPNHQMRVNKANPASLGIDTVKQTSGYLDFEDKHLFYYFFESRNDPVNDPVILWLNGGPGCSSMTGLFFELGPSSLGPELKPIHNPYSWNNNASVIFLEQPVGVGYSYGDTKIGSTYAAAKDVFVFLELFFQKFPSFSQNKFHIAGESYAGHYIPNIASEIVNHADRSFELDSVLIGNGITDSLIQDAYYQPMACGLGGYKQVLTDEACDQMIKDYSKCKRLVKACYATKSAFACVPASVYCGDKLVGVYQKTGLNPYDIRAPCETDNGLCYEGMDYIDSYMNQKEVQEALGAEVDSYEGCSNDVSASFILTGDESKPFQGYVAELLEKNVAVLLYAGDKDFICNWLGNHAWSNALDWKHKEEFSNQFLQPWYAGANHTAAGEVKSFANFTFLRIFDAGHMVPYNQPEVSLNMLNTWISGDYSFSAYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV83</accession>
    <name>A0A1E3NV83_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_86031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036316.1">
        <property type="nucleotide sequence ID" value="XM_019185985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV83"/>
    <dbReference type="STRING" id="683960.A0A1E3NV83"/>
    <dbReference type="GeneID" id="30203231"/>
    <dbReference type="OrthoDB" id="10017101at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="34"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="259" mass="29989" checksum="A33BDDC3056AAC09" modified="2017-01-18" version="1">MLLFELYGYESTISQPHAWRTAKNSSGYMLKYIKPTDKILDVGCGPGMITYDLSNYVPEGEIIGIEPTRELIDEALNNEKSDAVKNVKFQVASVFELPFPDNYFDIVHAHQVIIHLQHPIEALKEMRRVLRPDGYLCFKDAELRSTIYYPRKYEDPLGYYMTKIRTEFTSCFGASRSKELTLEAGFDPENIVFTSSNWTISSEDDRRWFSQMYINRLESLEFGKSDKYTSEQLELAWKEWASDDNAVCILLHGEIVAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVD4</accession>
    <name>A0A1E3NVD4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rad9-like Rad53-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_70957</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ56990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3NVD4"/>
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        <property type="entry name" value="Rad9_Rad53-bd_dom_fun"/>
    </dbReference>
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        <property type="entry name" value="Rad9_Rad53_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad9-like Rad53-binding" evidence="2">
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            <begin position="609"/>
            <end position="671"/>
        </location>
    </feature>
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            <begin position="1"/>
            <end position="31"/>
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    </feature>
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            <begin position="204"/>
            <end position="254"/>
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        <location>
            <begin position="266"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="440"/>
        </location>
    </feature>
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        <location>
            <begin position="509"/>
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    </feature>
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            <end position="253"/>
        </location>
    </feature>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="677" mass="75624" checksum="2CCCCB0AAF04A7B1" modified="2017-01-18" version="1">MSKQGTQTSPEANTSDSRPSQANGTYNQFLNDTQVIPFTKPESLIGSNSQPSRIQNLRVLSENEDNRIIDSSCDFKDSISTQALLDKEPDTVVIKEDIPVTERNEDESIRLSTAESSAPFKNLKLPDGESPWLSRVKDPFNDSETPWLNKIRDPFKETPINEKVLDAMGETPNSTRKVLVNSLNTPSAAHKIFQKLREKGNQKELDFDSQTVPELFKDTQPPSLEKEHEGSNNDVTMDLNHEIDSPRKDHFTDDVPTQVVNDTLIIGDPNQHSEPQDPKSDNTQADIQVPGTAPEVDHSLVIENDEISKDLDAGESSPINSDDEKNFSLDYGRRLHSEPVSGDYLNILAEDSIQKYHRDLTKRGLQLENQSKSFAGDTQRISQSKRLNSLPLNLDTDTLHQAKITVQGNKNTQEQGAPKQVTKSHLTSSPNVEESSPAIREKADTFEIEKAKADDNNNIETNTINTSNKSFHSQAVDFNALAENITQEFSDVDDESKVVDNTIEEEADNLKLQTEWNEGTSKSSNPVIRPTRNSKRKKSSKANDLQLQFTSSPDKSINHSDLSIVLDANTSQIDDNDHDISTNDQQSILTPEYDVNVLPNGILREETHNLSEEDIRFKDSVWCLANTRYYPGLILSLGKKGLEVKFFESTDEVNGGIFPLDIQIGDTVKIGRLPYAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVG2</accession>
    <name>A0A1E3NVG2_WICAA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
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            <end position="103"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
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        <location>
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        <location>
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        <location>
            <begin position="338"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ57084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="438" mass="49204" checksum="6293DF4D6F0B7084" modified="2017-01-18" version="1">MSLNTILRKFIQLRLSLSFRDLLPSPIIEHEAPIVPTISATIPTEIPPTEPAEDAETHLRSVPTTRVSQLRRFLDFVSRRGRRRRASTQQQQESTQQEQQHQQIAFSNSLGHESATVLAFSLSSESVNRDTNLRNPELFNFGTTVEPIQIRRDESSYHIIPLSPTRPNTRLPFNVMEDSPLSTLSDPEEIAQLPPSRIPPIEFIAQSGSELGGSGLRDSPNNYILLTDTQASEQERATSGHMRDGSSGRMSSSQSSVLTPDIQPSLFENFSIDNSSVRSMTGVSGEWDHPQLLRTSPPPRSRHRRLSLSSSFFSFRRRRDILSGTRLSTLSEGEDESSSPVPTHQPDENTLNEFTKTQRFNLIRHSLGRSRDLYKMLDIHHYSRHSNTGTPMTPSPVKAESQAHNTPTDEEILNKDLNFDSNIPIRPLAASDLDLSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVH5</accession>
    <name>A0A1E3NVH5_WICAA</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
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                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
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                <person name="Kurtzman C.P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="ODQ57040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="294" mass="32737" checksum="9997907E9A85204F" modified="2017-01-18" version="1">MSFKPQRGTVHEYASRLTAFEFDHGDKESPNAIIFIGGLGDGLLTVPYVPTINEKSLPDGWSIFQIHMSSSYIGWGTGSLDRDIKEIGLLVKYLRTKLNKKKVILFGHSTGCQDSIHYLLNRGSDIDGIVLQAPVSDREAMVMGFEEAGKSLTEFNHEARYVYEKHGPDALLPKKFSKEFFGAPINAYRWISLSEAGGDDDYFSSDLADSQLKETFGKIDKPLLVLYSGSDEFVPPKINKEAVVERYKSFTDPKYWSKYGGLVKGAAHNVGPQSEPGAVDILVEKVKGFLSEFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVJ2</accession>
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        <dbReference type="NCBI Taxonomy" id="683960"/>
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            <taxon>Wickerhamomyces</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036388.1">
        <property type="nucleotide sequence ID" value="XM_019185228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVJ2"/>
    <dbReference type="STRING" id="683960.A0A1E3NVJ2"/>
    <dbReference type="GeneID" id="30202474"/>
    <dbReference type="OrthoDB" id="1938992at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033255">
        <property type="term" value="C:SAS acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038988">
        <property type="entry name" value="Sas4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029184">
        <property type="entry name" value="Sas4_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38422">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38422:SF1">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15460">
        <property type="entry name" value="SAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Something about silencing protein 4" evidence="2">
        <location>
            <begin position="107"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="285" mass="34053" checksum="D079240753CF78D2" modified="2017-01-18" version="1">MTRTRINTKQGEPSAPRKLRSKETPKIDSSLFNYEENPNIIDPDQRIFIVDEKEVGEVLQNDIKVDQKSFSFETNLKTVDDLNNKKNGSSKRRKLRKSTITRQQPTDPLNDELYIPYNRRMEKEEKKMINWEREKIYSEADKMKAQLEKLHQNDWMKSLQTITYIRDPRDHKEMMEKKEWTIESLKGMLKRFEDWKRCEDRVLGRIRASSPSLIQHPFKYYTNMPDADLVQDSDTDEDENNMTIPQIRRLRLRKKLEKFGPIIKVKFGTKVIIAEPFKATRIENR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVS4</accession>
    <name>A0A1E3NVS4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_65472</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036420.1">
        <property type="nucleotide sequence ID" value="XM_019183916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVS4"/>
    <dbReference type="GeneID" id="30201162"/>
    <dbReference type="OrthoDB" id="3978317at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="95" mass="10619" checksum="82DFA79AE838885A" modified="2017-01-18" version="1">MPLVINTAPVELTTTEGYNEFIKSPLCNEHSTIFPNETSDQGYVYVNDEVVAIVRGEARDSLFNYTSSNLVRDDKESIVSVISFTKVFITLLLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW49</accession>
    <name>A0A1E3NW49_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosylphosphatidylinositol anchor biosynthesis protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_106890</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGF family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036565.1">
        <property type="nucleotide sequence ID" value="XM_019180483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW49"/>
    <dbReference type="STRING" id="683960.A0A1E3NW49"/>
    <dbReference type="GeneID" id="30197729"/>
    <dbReference type="OrthoDB" id="3980320at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009580">
        <property type="entry name" value="GPI_biosynthesis_protein_Pig-F"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06699">
        <property type="entry name" value="PIG-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ57358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="220" mass="24698" checksum="6EB962F0FBBFDDE2" modified="2017-01-18" version="1">MAARHTPKKTVSFSDNAPPTKESNGELDDGISIPFSLKTIPFHYLLIVFSILKQDITRSNVMNNLTNNLYALIATQLVYTFLFVTNIVELTKKKNNKKIKLSVGKDYNILLISILIAFVLILPIYILVVLFGAPFTTFLKENLILSSHIAFLAYPIIVSFLKIGEKGVFHKYLAFIAVGAWFGAIVIPLDWDRDWQNWPLPIVISSYIGSFIGYSIAGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWH0</accession>
    <name>A0A1E3NWH0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial GTP/GDP carrier protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_96782</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036681.1">
        <property type="nucleotide sequence ID" value="XM_019186536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWH0"/>
    <dbReference type="GeneID" id="30203782"/>
    <dbReference type="OrthoDB" id="409947at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001409">
        <property type="term" value="F:guanine nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000010">
        <property type="entry name" value="Probable YHM1 (Mitochondrial carrier)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053042">
        <property type="entry name" value="Mito_GTP/GDP_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46974">
        <property type="entry name" value="MITOCHONDRIAL GTP/GDP CARRIER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46974:SF1">
        <property type="entry name" value="MITOCHONDRIAL GTP_GDP CARRIER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="215"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ57474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="304" mass="33004" checksum="4287E46C74107575" modified="2017-01-18" version="1">MSPAAHSTTDKKQSGLARVLGSASAGICEIGVFHPVDTISKRLMSNSTKVTNLSQLNSVIFRDHAAEAFGKRVFTLFPGLGYAAVYKILQRVYKYGGQPFANEFLNKNFKSSFEDNFGPKTGKALMSATAGSIIGIGEVVLLPLDVLKIKRQTNPEAFRGRGFIKILSDEGLGLYRGWGWTAARNAPGSFALFGGNAFAKEYVLGLKDYSQATWSQNFVSSIFGASASLIVSAPLDVIKTRIQNRNFENPESGFTILKNMAKNEGITAFFKGLTPKLLTTGPKLVFSFALAQSLIPTFDKLFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWI0</accession>
    <name>A0A1E3NWI0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dienelactone hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_35794</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036766.1">
        <property type="nucleotide sequence ID" value="XM_019182357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWI0"/>
    <dbReference type="STRING" id="683960.A0A1E3NWI0"/>
    <dbReference type="GeneID" id="30199603"/>
    <dbReference type="OrthoDB" id="58297at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47562">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47562:SF2">
        <property type="entry name" value="CARBOXYMETHYLENEBUTENOLIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dienelactone hydrolase" evidence="1">
        <location>
            <begin position="23"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="272" mass="30788" checksum="14AB33CB75648B88" modified="2017-01-18" version="1">MLITESYHDVKTSYGTTLRLYVYHPKIPNYPRAKFPGVLVYSEIYQVTGPVSRFAKKIASEGYICVAPSIYHNFEGPEPLNYDAEGTDRGNEYKVKKPLESYDEDNKLALDYLESLETYSGKCGATGMCLGGHLAFRSVLDKRISASVCFFPTDIHDHALGAGENDDSLKRAEEMSRKDVEIVLIFGTLDNHVPPEGRDLIRKTLRDHDVNFTFMELNDAQHAFVRDESSKGRYDSAITSACFGFLFELFNRRLRTDLGENDGKEIEVEHVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWL7</accession>
    <name>A0A1E3NWL7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase eIF4A</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Eukaryotic initiation factor 4A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_46746</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. eIF4A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036778.1">
        <property type="nucleotide sequence ID" value="XM_019182598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWL7"/>
    <dbReference type="STRING" id="683960.A0A1E3NWL7"/>
    <dbReference type="GeneID" id="30199844"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18046">
        <property type="entry name" value="DEADc_EIF4AII_EIF4AI_DDX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000089">
        <property type="entry name" value="Eukaryotic initiation factor 4A-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044728">
        <property type="entry name" value="EIF4A_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="18">
        <location>
            <begin position="23"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="54"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="235"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="14">
        <location>
            <begin position="23"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ57571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="397" mass="44743" checksum="C5C8381B2EE88AE8" modified="2017-01-18" version="1">MASDGITEVDANLIESNYDNVVYTFDDLNLKKDILRGIYAYGYENPSSIQQRAILPITEGRDVLAQAQSGTGKTATFTISALQRIDENEKATQALILAPTRELALQIQKVVLSIGLHLNVTVHASIGGTSTKDDIDALKAGAQIVVGTPGRVFDMIDRRFMRTDKIKMFILDEADEMLSSGFKEQIYNIFRLLPETTQVVLLSATMPQDVLEVTTKFMRNPVRILVKKDELTLEGIKQFFINLEEEQYKLDCLLDIYDSISVTQAVIFCNTRRKVEFLTEKLTENKFTVSAIHSELSQQERDTIMNEFRTGSSRILISTDLLARGIDVQQVSLVINYDLPSNKENYIHRIGRGGRFGRKGVAINFVTDKDVGMMREIERFYSTQISELPGDIGEIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWV2</accession>
    <name>A0A1E3NWV2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin-conjugating enzyme E2 6</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">E2 ubiquitin-conjugating enzyme 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Ubiquitin carrier protein UBC6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Ubiquitin-protein ligase UBC6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_35562</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="15">Protein modification.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036858.1">
        <property type="nucleotide sequence ID" value="XM_019182328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWV2"/>
    <dbReference type="STRING" id="683960.A0A1E3NWV2"/>
    <dbReference type="GeneID" id="30199574"/>
    <dbReference type="OrthoDB" id="1158011at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23799">
        <property type="entry name" value="UBCc_UBE2J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000023">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 J2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="UBC core" evidence="17">
        <location>
            <begin position="5"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00043952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ57651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="238" mass="27427" checksum="36576FFB72083174" modified="2017-01-18" version="1">MATKQAHKRLTKEYKSMVENPPPFIIAHPSERNILEWHYLISGPPDTPYAGGQYHGTLMFTSEYPFKPPAIKMITPSGRFQPNTRLCLSMSDYHPDLWNPAWSVATILTGLLSFMTGDEDTTGSIRTRDESKKKFAKASLTFNLNSERFLREFSDLVDETRTKIDEWDKNEAKIEDEKKRKLANDNKPEEIVDPSTLDPEDRIRYEQARNRAVEGQKSNFVYYVLAIAVALGLFRYIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWY1</accession>
    <name>A0A1E3NWY1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_85779</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036853.1">
        <property type="nucleotide sequence ID" value="XM_019185945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWY1"/>
    <dbReference type="STRING" id="683960.A0A1E3NWY1"/>
    <dbReference type="GeneID" id="30203191"/>
    <dbReference type="OrthoDB" id="289247at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000309">
        <property type="entry name" value="Chromosome 21, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040250">
        <property type="entry name" value="Nucleobindin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19237">
        <property type="entry name" value="NUCLEOBINDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19237:SF20">
        <property type="entry name" value="NUCLEOBINDIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133540" description="EF-hand domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="100"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="218" mass="25168" checksum="5D233923D523BDD0" modified="2017-01-18" version="1">MKLRRTTLLPLASIISLSLAHDLKEKPPQGLSWEEWHMKAEHGLDEYDPESFFTLHDSKNKKYLDSDDILNAYGLHRDEVVGKGDGLGSHDDSESISAETKREIVSKILKLLDSDSDEKVTLDEYLKFAEKGGKFPDLGVGVGHHGDFEHEYEVHHWNKYHKDQDPDIKIIHKEDIEHELLHHEHEIEHEVDPNAAKMSDEELESRIILQNIPPKYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX16</accession>
    <name>A0A1E3NX16_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_70722</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036842.1">
        <property type="nucleotide sequence ID" value="XM_019184212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX16"/>
    <dbReference type="STRING" id="683960.A0A1E3NX16"/>
    <dbReference type="GeneID" id="30201458"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16473">
        <property type="entry name" value="RING-H2_RNF103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051826">
        <property type="entry name" value="E3_ubiquitin-ligase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765:SF416">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE GODZILLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765">
        <property type="entry name" value="RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="223"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="140"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="379"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="140"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="154"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="379"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="424" mass="48298" checksum="C81E78B9C1DC2D6C" modified="2017-01-18" version="1">MSTQNNNTSATSQPSSILFFIAVAIGVVIALVFIFFSMRYYVRTRLGIYATARLTTTSNNGGIVLLTGDFPMHSFNGYSGDLPNQLRRRNRRRKYLKKRRLTEEEVDHLFPVRTYQDWLDGGAERDADQREIHVMLKEENDDEVVEQQQDEQTPEPQNQTQDGSTADGRTITATDTIATSDTPAIKVNTPDHDTGSTYHDALDQPEQVDLGTPYEPHYDSGTCAICIDTFEPEDPVRGLICGHVFHQECLDPWLTKRKACCPMCKRDYYLKDESDEQQAQGNGAEPENQIDDLDSINQFTELQYTTLNERVTEILNRHPDLEQIATEKIKKYLKFKWRVFWLIMGISKSDLVNSAIVNEDQRLREEDPTYDVINANEVINNGNNDGNANEANNNDDNNEGNRDGAGVQTVENSQEIRDRVERMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX50</accession>
    <name>A0A1E3NX50_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="9">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_35535</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="9"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036920.1">
        <property type="nucleotide sequence ID" value="XM_019182322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX50"/>
    <dbReference type="STRING" id="683960.A0A1E3NX50"/>
    <dbReference type="GeneID" id="30199568"/>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007129">
        <property type="term" value="P:homologous chromosome pairing at meiosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000012">
        <property type="entry name" value="DEAD/DEAH box DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052247">
        <property type="entry name" value="Meiotic_Crossover_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47835:SF3">
        <property type="entry name" value="HELICASE FOR MEIOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47835">
        <property type="entry name" value="HFM1, ATP DEPENDENT DNA HELICASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0469">Meiosis</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="51"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="265"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ57713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="851" mass="96687" checksum="88750BBCEF9BF142" modified="2017-01-18" version="1">MSFDNTSDTSNIHHFNTSTNDSRLPTSVLSDKARSIFDFHYFNKMQSKSFKPIYEEDNNCVISSPTGSGKTVLFELAILRLLNLTNDPNTLKVLYISPTKALCLEREKDWCSKFSSYGLTVGALTSDTSYLETDKVKKANIIITTPEKWDLVTRKWADYSKLFKLIKLLLVDEIHILRDNRGSTLEVVITRMKKICRPLRIIALSATVPNIQDVSGWIKLNSDSGQNAVTLVFGDEYRPVKLEKLVFGYRQTMNDFVFDSFLNNKLAEIIRQHSNNKPVLIFCPTRNSTISTAKYLSKNLRVEIKSQSPALIKEKELKELSMLGFSYHHAGLSLPDRLSVEKNFINGNTKVLCSTSTLAVGVNLPAYLVVIKGTKTWTGAAFEEYSELDILQMMGRAGRPQFETQGKAVLMTDSDKQHQYEKLVKGTEKLESRLHLNLSENMVPEVFLKTVNSVEGALEWLKLTFFYTRFKNNPTAYDEIPYLANGSMDARLLKYVESKLRELTDFKLIEIKNGEYQCTQFGNAMTKHYILFDTMKMFVKCPRGLTVSDIINLVSKSSEFENIRLKRTEKKLFRELNNSPILKYPIKTYNTLSNITSNDEKINLLVQYELGGLEYPNGQEFLKIQQSFKQDKFFVFKHIGRLIRCLVDCFLEKADYVSLLNCLKLSRCLNGKCWEDTSIVLRQLEGVGIAAVRRFSNQNVKSFSEVKNLSSSTIEYYLNQRPGFGAKVLKAIAGLPILSIETHLESSTVTKGELKVIIRMNIDCQMETKSWHGKPVTVNIVSGLSTGDLVDFRRAHLNKLSGAKSFTFECSVLYKNTSLTSSVSCEEIGKLILFSVKGDFTNGSSWYFSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX52</accession>
    <name>A0A1E3NX52_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Small-subunit processome Utp12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_85785</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat WDR3/UTP12 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036882.1">
        <property type="nucleotide sequence ID" value="XM_019185946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX52"/>
    <dbReference type="STRING" id="683960.A0A1E3NX52"/>
    <dbReference type="GeneID" id="30203192"/>
    <dbReference type="OrthoDB" id="407922at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001139">
        <property type="entry name" value="DIP2p Nucleolar protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000157">
        <property type="entry name" value="WD repeat domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000178">
        <property type="entry name" value="WD repeat domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051570">
        <property type="entry name" value="TBC1_cilium_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN 3 WDR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25172">
        <property type="entry name" value="Beta-prop_WDR3_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="78"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="113"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="155"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="376"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="457"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="556"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="598"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="640"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="8">
        <location>
            <begin position="786"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="696"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="927" mass="105266" checksum="02CC8E2006D223BD" modified="2017-01-18" version="1">MVKSYQRYEQKSCFGVISSKSNIIWIPPSNKTSAGQAITAGLEEILIWDIKTGELVKKLTDGLPIGAFDSKLTKPSEVTSVELHQDTNLLAAGYEDGSIKIWDLISGTVLINFQGHKSAITILSFDKEGTRLISGSRDSNIIFWDLVGETGLFKLRSHKDQITGLWFNEDWLISTSKDGLIKLWDLQTQQCIETHVAHTGECWNLALMDDILITTGAENQAKVWKIDSEEENNKLTERGVFEKQSKARGLEVSFAKVGSNRFFYIQNADKTIELFRIRTTEEIAKATKKREKRLKEKGYDEEEIENAIRESDVNMLIQPFTVLRSIFKIKNATWALTNSTKLELLLSSSNNTIEYYTIAYTNKEVTAPSKTFTVDLQGHRTDIRAMDISDNDKLLATASNGLLKIWNTRTNNCIRTFESGYALATKFLPGGSLIVVGTRAGELELYDLATSTLLETTQAHDGAIWSLDITPDGKSLVTGSADKTIKFWDFKIEQELVPGTERMAAKMKIFHNKTLELTDDILSLKISPDTKYLAVSLLDNTVKVFFFDTLKFYLSLYGHKLPVLSIDISHDSKLIITSSADKNIKIWGLDFGDCHKSIFGHQDSIMNVRFLPESHNFFSGGKDGLLKYWDGDKFECIQKLSAHQSEIWSIAISTDGSFVVSTSHDHSIRIWEETDDQVFIEEEKEKEMDELYEGTLLASLEGEDEPKKDDDEEEDKDEATGVNQQTLETLKAGEVLLEALEIGGKDMEDEEEYQTAYKKWLKTKTGEQPIKPSKHILIQSKNVSGSEYVLDQLLAIKPAQLEDALLVLPFSFVLKFFKVIERWTRSNKVLRQHTSIICKVLFFLIKSNLYELNSMKDESIKKLITSVRSQLRAELKQSTNELGFNIQGLKVLQTQWNLTHNAEFIDEYENREQVEKQSKKRVYQTLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXG6</accession>
    <name>A0A1E3NXG6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_64051</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037106.1">
        <property type="nucleotide sequence ID" value="XM_019183662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXG6"/>
    <dbReference type="STRING" id="683960.A0A1E3NXG6"/>
    <dbReference type="GeneID" id="30200908"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF3">
        <property type="entry name" value="RING ZINC FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="110"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="13"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="182" mass="21099" checksum="90F1E1D80E733E9D" modified="2017-01-18" version="1">MLRYNRYMSTYEEEHNIRENRTPSQQPSRTHETLSSRLEHFLEGRENGTTTQQGQVELLVSALRSLNNGEFGDSLLDQLNSEKDIQGVPEEFIDSLDRVSKKSLKKDQTCSICTNDFLDDPHPLVVRLPCDGNHKFDLECISPWLKVHSTCPLCRKNLLDRKKFDDLPEDDEEVEEGWDIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXL3</accession>
    <name>A0A1E3NXL3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS10m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">37S ribosomal protein S10, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_15448</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037105.1">
        <property type="nucleotide sequence ID" value="XM_019180645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXL3"/>
    <dbReference type="STRING" id="683960.A0A1E3NXL3"/>
    <dbReference type="GeneID" id="30197891"/>
    <dbReference type="OrthoDB" id="366214at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.600:FF:000003">
        <property type="entry name" value="30S ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_uS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01049">
        <property type="entry name" value="rpsJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS10" evidence="6">
        <location>
            <begin position="29"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="180" mass="21175" checksum="6D1312B8D44A83D7" modified="2017-01-18" version="1" fragment="single">NNRPLPVNVEANYWAPMKHKPKYNDKVAELQLRSYDAQLVEFYGDFIIRAAYYLNIPVSGTIPLPKRRERWTVIKAPFVHAKTKENYQRITHKRLIKAYDTTPEVVDLWVSFLRKHSPAGLGIKVQTYQKQSLEFLEELDKVDTKQLDDTLDYNALSENSDVKKRVEQLLNDPVFKQHFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXN5</accession>
    <name>A0A1E3NXN5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thiamine thiazole synthase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Thiazole biosynthetic enzyme</fullName>
            <ecNumber evidence="6">2.4.2.60</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">THI4</name>
        <name evidence="7" type="ORF">WICANDRAFT_80033</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP-thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:55708"/>
            <dbReference type="Rhea" id="RHEA-COMP:14264"/>
            <dbReference type="Rhea" id="RHEA-COMP:14265"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:90873"/>
            <dbReference type="ChEBI" id="CHEBI:139151"/>
            <dbReference type="EC" id="2.4.2.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="6">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homooctamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">During the catalytic reaction, a sulfide is transferred from Cys-206 to a reaction intermediate, generating a dehydroalanine residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the THI4 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.60" evidence="6"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037072.1">
        <property type="nucleotide sequence ID" value="XM_019184989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXN5"/>
    <dbReference type="STRING" id="683960.A0A1E3NXN5"/>
    <dbReference type="GeneID" id="30202235"/>
    <dbReference type="OrthoDB" id="410463at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160205">
        <property type="term" value="F:cysteine-dependent adenosine diphosphate thiazole synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03158">
        <property type="entry name" value="THI4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027495">
        <property type="entry name" value="Sti35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002922">
        <property type="entry name" value="Thi4_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00292">
        <property type="entry name" value="sulfide-dependent adenosine diphosphate thiazole synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422:SF3">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01946">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="98"/>
            <end position="99"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="302"/>
            <end position="304"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="2,3-didehydroalanine (Cys)" evidence="6">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="327" mass="34962" checksum="97E4E8AF8F4E6E31" modified="2017-01-18" version="1">MSSTIQTVTEQASNLHISGTPPIRHALSDVVKTEDWSDFAFAPIRESTVSRAMTSRYFKDLDKFAVSDVVIVGAGSSGLSAAYVLAKNRPDLKIAIVEANVASGGGSHLGGQLFSAMIMRKPAHLFLEEVGVEYEDEGDYVVVKHAELFISTIISKVLSFPNVKLFNATAVEDLVTRTHPETGNITVAGVVTNWTLVSLNHDTQCCMDPNVIELAGYKNDGSRDENVKHGVVLSTTGHDGPMGAFSAKRIVSIDKRKSLGGMRGLDMNAAEAGIVKYSGKYDGVDGIYFAGMEVAELEGTNRMGPTFGAMAVSGIKAAEEILKHFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXU8</accession>
    <name>A0A1E3NXU8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">U3 small nucleolar RNA-associated protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_64160</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the ribosomal small subunit (SSU) processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the HEATR1/UTP10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037218.1">
        <property type="nucleotide sequence ID" value="XM_019183679.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3NXU8"/>
    <dbReference type="GeneID" id="30200925"/>
    <dbReference type="OrthoDB" id="31183at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
        <property type="term" value="C:t-UTP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012954">
        <property type="entry name" value="BP28_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056473">
        <property type="entry name" value="HEAT_Utp10/HEAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022125">
        <property type="entry name" value="U3snoRNP10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040191">
        <property type="entry name" value="UTP10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457">
        <property type="entry name" value="BAP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457:SF1">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08146">
        <property type="entry name" value="BP28CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23243">
        <property type="entry name" value="HEAT_HEATR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12397">
        <property type="entry name" value="U3snoRNP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01036">
        <property type="entry name" value="BP28CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 9" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 9" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="BP28 C-terminal" evidence="11">
        <location>
            <begin position="1470"/>
            <end position="1617"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="8">
        <location>
            <begin position="1708"/>
            <end position="1746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="814"/>
            <end position="833"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ58011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1748" mass="195035" checksum="90E42FA21A6DBB60" modified="2017-01-18" version="1">MSLASQLGSIAAANSTVAFDRKRRQKLHSVSLIYNSKVAATQDYEIIYSNAYEALEELITIDKRFSFFKNSLFSETSVSVDRNVQTAEQNKDLDNAINAYLSLISPRWNLNPAVQATEWLVRRFQIHIFNAENLLLSTINYYQGPVFKRILEITKLPPLFSGLAGFKNTERSPTNTSMIKLFTDLDFLNLYIKYLEDGATKKILYSNQLLFFTCSAINSIAILSNDPKKVEQLIPLILEVSAKFLASSNSDAHVASHTVLAVLSTAVPLSQEIIYAATETILANIQKKVENSGLICITKLFQSLNSSKYENIPVRIYRLLIKLITGDLERFDEILTSKTIHSEKLVTVLVKTILAYEFDSRIPLVLSILKKVSLPEYEMSYIIRDSITAIDVIQQDKSDITELIEYYIKNQKDLLLDGLNVMSLTPDLLEAKLQTSLSLTGKEDVENTPVQEVITETTEESKAKTIELFKSNKTKVVSFLTADLNAEFNKLLPLYIKALQSKVTEEFQNEVFESSDGLYTFLVRVGISPSAPGFARTSALAAFKSLIPKIDPSVNLLTLVPVLFAGLYDTRRSVRGLSSDILKIISQRKVTDKFYLENSIFGSKSKELQLISPKDGESFLKAVLENYFVESAGVSDILVNAKKNAKLFLAFLANQANVISLPSVKLVLLKIIRVSIASVKGAVLSQIFQSLLENYVAKRESFRKNVEENKVDLKDFETEVVNLVSAKEKNVFAINFLVNCLSSPYEGLAELASARSIAIFESLKYDFKLKLAKSVVDSFATEGSTSYDALLTLQSLPLSSEIIVALLKDSQINQPKDEPGVPKRRRRSSASARQALNTGELAKIAENHLQKITVILETLDQSLDTLKPSTTLLSALFNLLSDLETLGGDAGLPVLYTQETLAVTMVKVIRALKSDKAQLDSNSVRTDIVVATIRSSPSPQVQNRLLLVVAELAALAPEIVLHSVMPIFTFMGAHTIRQDDEFSVHVVEQTVVHVIPALANASNEKKSDEVEFLLTSFASAFIHIPRHRRVRLFSTLASTLGPELSLHIILFLIGGQYSAAVLKNRTSESRSLIDFASSLLKQFTAVEQLAALNSFIEIWKKIPTEQITRESPNADMFYSSPIFNSTVVSSTSEELLSLKSNLLEFIDSSLNGGESVAIPPLQLKITSILYDESETELQSADIFQTFGDLVQSLLQLIGEKSKSDISSNLFKLLSDVLTLLPIKEFVSSISNLLSDSQLDVVVRQNLTLLSLEKFEAESSEREEAQFAANNLIEVLFTNIKSNEDLAQASLDALASLIQKFGSQVDDELLTKSLHLSTSDAGLLSSNGEAIVSSLTVITNIVSVLGIKTISFFPKIIPPSLKIFNESFQIEDEEAKGTLQLSVLLLLSSYVKRIPAFVTTNLQDILRCIFKADEVSEKIRGSIVQVIVQNMDHTALLKVLASIWDETSKLNATSIGIYLNTLESTVEVIEKKTATSQAPKFFNLLLRLFEFRSESDFDNNAIHRIEAFFHDIANKYVMKLNDKTFRPLFALLVRWAFDGEGVTNADITEVERLTSFFRFFNRLQENLRSIVTSYFTYFFEPTIGILKRYISAELDDVNLRRIVLNALTSSFKYDQDEYWQAQARFEVVSESLLSQLQNVEDSIGKYLVKSIAALAQNASSDEHNKALNQLFISHMKSECKPKEKLWATRSLKSVYQKVGDQWLTLLPQLVPIIAELLEDDDEDVEMEVRTGLVKVIEAVLGEPLDRYLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY06</accession>
    <name>A0A1E3NY06_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 17">mRNA-capping enzyme subunit alpha</fullName>
            <ecNumber evidence="3 17">2.7.7.50</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 17">GTP--RNA guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 17">mRNA guanylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">WICANDRAFT_64196</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16 17">Second step of mRNA capping. Transfer of the GMP moiety of GTP to the 5'-end of RNA via an enzyme-GMP covalent reaction intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 5'-end diphospho-ribonucleoside in mRNA + GTP + H(+) = a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:67012"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="Rhea" id="RHEA-COMP:17166"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167617"/>
            <dbReference type="EC" id="2.7.7.50"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:67013"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Heterodimer. The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an mRNA 5'-triphosphate monophosphatase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the eukaryotic GTase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.50" evidence="3 17"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183688.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="30200934"/>
    <dbReference type="OrthoDB" id="200924at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:mRNA capping enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:GTP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:mRNA guanylyltransferase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:RNA polymerase II C-terminal domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07895">
        <property type="entry name" value="Adenylation_mRNA_capping"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000542">
        <property type="entry name" value="mRNA-capping enzyme subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="mRNA-capping enzyme subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="mRNA_cap_enzyme_adenylation"/>
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        <property type="entry name" value="mRNA_cap_enzyme_alpha"/>
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        <property type="entry name" value="mRNA_cap_enzyme_C"/>
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        <property type="entry name" value="mRNA_Capping_Enz/RNA_Phosphat"/>
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        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367:SF17">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03919">
        <property type="entry name" value="mRNA_cap_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01331">
        <property type="entry name" value="mRNA_cap_enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036959">
        <property type="entry name" value="mRNA_cap_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 17" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="5 17" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="mRNA capping enzyme adenylation" evidence="20">
        <location>
            <begin position="42"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="mRNA capping enzyme C-terminal" evidence="21">
        <location>
            <begin position="240"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="373"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="383"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="414"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="active site" description="N6-GMP-lysine intermediate" evidence="18">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00023134"/>
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        <source>
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            <dbReference type="ARBA" id="ARBA00044624"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00053845"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR036959"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR036959-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF03919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="ODQ58048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="443" mass="52380" checksum="74B049D505BB9585" modified="2017-01-18" version="1">MLEERDFPEIPGELMPDSIAHEVKQQVARLLNRRRLEFPGSQPVSFEAKHIRENLEGRDYFVCEKSDGLRCLMLCILDPNGEEAVFLITRENQYFRIPHLHFPLPEDENSCHNGTIIDGELVLSKRPTDGVKELRYLMFDCLTLNGKNIVTRPLDKRLGYLSEQFYKPYFKLREHHPKECEDFPFKLSLKNMHFSYKIPKVFENLKYLGHISDGLIFTCRETPYVFGTDPTLLKWKPSEENTIDFKLHLNIPLYTDLDLDERDPYRSYPNYDVKPGFELLVWDGKDQYSNFSELAIGDEEWEKLKSLEEPLEERIVECHKDKEDNWRLLRFRDDKANGNHATVVQRVLESIRDGVTKEDLIKESPTIERSWKQRELNRKSRHQQFQQHQSPHTQQQPSQHIPNGAPPSKKRPQEDDELLPTYEEEDDDSEDNEEHESKKPRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY41</accession>
    <name>A0A1E3NY41_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Isocitrate dehydrogenase [NADP]</fullName>
            <ecNumber evidence="11">1.1.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_85133</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19629"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="11 14">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="11 14">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="11"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037181.1">
        <property type="nucleotide sequence ID" value="XM_019185843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY41"/>
    <dbReference type="STRING" id="683960.A0A1E3NY41"/>
    <dbReference type="GeneID" id="30203089"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006739">
        <property type="term" value="P:NADP+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000002">
        <property type="entry name" value="Isocitrate dehydrogenase [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004790">
        <property type="entry name" value="Isocitrate_DH_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00127">
        <property type="entry name" value="nadp_idh_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006156">
        <property type="entry name" value="PRK08299.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822:SF41">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NADP]-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822">
        <property type="entry name" value="NADP-SPECIFIC ISOCITRATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000108">
        <property type="entry name" value="IDH_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="6 11 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 11 14" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11 15" id="KW-0521">NADP</keyword>
    <keyword evidence="8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="16">
        <location>
            <begin position="9"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="75"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="94"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="309"/>
            <end position="314"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="12">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="12">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ57974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="410" mass="46339" checksum="BCBBC9A98C4E3DA0" modified="2017-01-18" version="1">MSKITVKNPIVEMDGDEQTRIIWHLIRSQLVLPYLNIDLKYYDLSIEYRDETNDQVTIDSAEATLKYGVAVKCATITPDEARVEEFKLKKMWKSPNGTIRNILGGTVFREPIVIPRIPRIVPQWEKPIIIGRHAYGDQYRATDIIVPSAGELKLVFTPKDGGEVQEYPVFEFPGKGVGLAMYNTDESIEAFAESSFKLALERQLNLYSTTKNTILKKYDGRFKDIFENLYDTKYKPLFEAKGIWFEHRLIDDMVAQMLKSKGGYIIAMKNYDGDVQSDIVAQGFGSLGLMTSVLMTPDGKAFEAEAAHGTVTRHYRQHQQGKETSTNSIASIFAWSRGIIQRGKLDGTPDVVKFGELLEKATLDTVQVDGKMTKDLALATGHTERSSYVTTEEFIELVAKRLATVYKENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYM0</accession>
    <name>A0A1E3NYM0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pirin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_85300</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="2">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pirin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037420.1">
        <property type="nucleotide sequence ID" value="XM_019185871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYM0"/>
    <dbReference type="STRING" id="683960.A0A1E3NYM0"/>
    <dbReference type="GeneID" id="30203117"/>
    <dbReference type="OrthoDB" id="198735at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02247">
        <property type="entry name" value="cupin_pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02909">
        <property type="entry name" value="cupin_pirin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903:SF8">
        <property type="entry name" value="PIRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903">
        <property type="entry name" value="PIRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006232">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin N-terminal" evidence="4">
        <location>
            <begin position="22"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin C-terminal" evidence="5">
        <location>
            <begin position="175"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006232-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="34570" checksum="791B084F44B06EB8" modified="2017-01-18" version="1">MVSPRSILKYFLAVAQEEGVGAKVRRSIGTMQVRNFTPFLMLDHFNVSPPAGFPDHPHHGQETITYLISGKFAHEDFTGSKGILNPGDLQFMTAGKGIVHSEIPVAAEDGKPVTGMQLWVDLPKELKNCEPRYRDLRAKEIPVVKPNDKVTVKVISGKSYGTESVKELAYTPIDYYDFEVAPGGEFQQEFPKDFNVFLYVMDGSISINDTPIKQYNAVFFNTDGDGVVGKVPADAKETARFVIIGGQKLDQPIVQHGPFVETSREKMYEVFMNYQTGQNGFERARGFASSIASGVKEEDLVNVLTHIFFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYQ4</accession>
    <name>A0A1E3NYQ4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histone H4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_32681</name>
        <name evidence="13" type="ORF">WICANDRAFT_34084</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
            <strain evidence="13">NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the histone H4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038291.1">
        <property type="nucleotide sequence ID" value="XM_019181995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYQ4"/>
    <dbReference type="STRING" id="683960.A0A1E3NYQ4"/>
    <dbReference type="GeneID" id="30199241"/>
    <dbReference type="OrthoDB" id="3977856at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22912">
        <property type="entry name" value="HFD_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000007">
        <property type="entry name" value="Histone H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035425">
        <property type="entry name" value="CENP-T/H4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001951">
        <property type="entry name" value="Histone_H4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019809">
        <property type="entry name" value="Histone_H4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004823">
        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10484">
        <property type="entry name" value="HISTONE H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15511">
        <property type="entry name" value="CENP-T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00623">
        <property type="entry name" value="HISTONEH4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00417">
        <property type="entry name" value="H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00803">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00047">
        <property type="entry name" value="HISTONE_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="10 11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA box binding protein associated factor (TAF) histone-like fold" evidence="12">
        <location>
            <begin position="28"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ58130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ59084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="103" mass="11436" checksum="06481066FE62CDDE" modified="2017-01-18" version="1">MSGRGKGGKGLGKGGAKRHRKILRDNIQGITKPAIRRLARRGGVKRISALIYEEVRGVLKSFLENVIRDAVTYTEHAKRKTVTSLDVVYALKRQGRTLYGFGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ14</accession>
    <name>A0A1E3NZ14_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_64548</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Lipid-anchor</topology>
            <topology evidence="1 11">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037616.1">
        <property type="nucleotide sequence ID" value="XM_019183759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ14"/>
    <dbReference type="SMR" id="A0A1E3NZ14"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ14"/>
    <dbReference type="GeneID" id="30201005"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005621">
        <property type="term" value="C:cellular bud scar"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000936">
        <property type="term" value="C:primary cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905897">
        <property type="term" value="P:regulation of response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000005">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000038">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF2">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 11" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009027334" description="1,3-beta-glucanosyltransferase" evidence="11">
        <location>
            <begin position="22"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="13">
        <location>
            <begin position="376"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="482"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ58409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="545" mass="57520" checksum="0EE47867C3D99D61" modified="2017-01-18" version="1">MLFNLASSFVAAASLVSVVSAADLPAIEVVGNKFFYSNNGSQFYIKGIAYQQDTANITGGESFVDPLADADACKRDIPYLSAVNTNTIRVYALNESQDHTSCMQQLANAGIYVIADLSEPSLSIDRDSPSWTVELYNRYTKIVDEFHNYTNVLGFFAGNEVTNDNTNTDASAFVKAAVRDTKAYIKQQGYRSIPVGYSSNDDADTRIQIADYFACGDDDERADFYGINMYEWCGNSTFAKSGYADRTADFKNLTIPIFFSEYGCNEVKPREFTEVATLYGSDMTDVWSGGIVYMYFQEANDYGLVSIDGDDVKTLDDYNNYKSEILRVSPTSAASSAASASAHQLSCPTEDKNWKASTDLPPTPDDGLCSCMESSLSCVVGDSVDSDDYSDLFGIVCGEVSCDGINSNGTTGSYGAYSFCSSKQKLSFVLDLYYKAQGSAARACSFSGSASVKSASTQAACSTALAEAGASGLGTVSATVSGSGRSTATGSNGSSGSSSSAGSSSSSSGSSSRSGAAGPVVPRGEKVFASVMALLVVGGFTSILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ46</accession>
    <name>A0A1E3NZ46_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_106824</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037708.1">
        <property type="nucleotide sequence ID" value="XM_019180482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ46"/>
    <dbReference type="GeneID" id="30197728"/>
    <dbReference type="OrthoDB" id="3980718at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="688" mass="79776" checksum="8548E43887831963" modified="2017-01-18" version="1">MVYSRASVRLPNFLFTRVRHFHCSQTVFKKPNLNSVKFPFLKDKSPIIQAYQSDEKLPFNAMRDILKVPVVTPESLDRVSFAWLDYFAENDELNLQNHIKSETPEQFWMKTIRVAISDQQIMPQIVGFIRRNLKKKSFHVPESIGEIMVSSLIYSGHYKHAFNVYKDLLSYKESVNWNLILDVCLVLPDGSPLRVFETIHRDRNEGLYLKIISHLQRLKLKEQYIHSWNNKLVLLGDFPKDSSAPMNPVFNSMVETNNFRSFENCLKHMVRHLKREDIPHWLDDETSKYIAMCVKNAKDPASKVSHFISLYTPGSFKAQFFATLLHQTKIPSKKIMKLVSRAKIPENDTAIIEGKCKRLADSDPQAFWAYTDKNVEQLPPLKISIQILSLYLQNSDKLINAMNFAKDLILKYPEYTFKLARAFIMGVTNKAIETNHLAHHQLIAEFENFLRKEKLMSDEIENYLVFSELKLRHFESALMRIERLLAKPNAIIERGTIENTGRILSKNSRKLPRTFTAHGESQDHYIMRLILRMNNKCDEVSPQTWNVFLKQIIKNFDHTEVEKFCLEVIDCVKSQTELNFNSANAGHPLRLIFDDKLITSIMLHGLKDPLEPWSGLATLSKLQAKGVYIPMNLMKKRIIEVFRTSGLPNSDKKKVNSKVFITSNLGIDLILQKINEIEQSMSGTEQRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZV5</accession>
    <name>A0A1E3NZV5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_63145</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037842.1">
        <property type="nucleotide sequence ID" value="XM_019183497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZV5"/>
    <dbReference type="STRING" id="683960.A0A1E3NZV5"/>
    <dbReference type="GeneID" id="30200743"/>
    <dbReference type="OrthoDB" id="270624at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044285">
        <property type="entry name" value="PWP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14091">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14091:SF0">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="254"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="41"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="509"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="521"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="538" mass="60617" checksum="06CD49C43FA728B9" modified="2017-01-18" version="1">MLSSSTWVPRGFASEFPVQYELDDEEMERINKLAQLEINDAKQELAEEEEETTSSNKLTDQFEVDDDLKEYDLEHYDDDEDEDDGARVSMLPGLSNDAEYHENEDGEDPYISLPNQDEVVQDKKESQVYPTDNLILSTRTEDDISYLDIHVYDDGADEFADPEVPTGHERPNALYVHHDLMLPAFPLCVEWINFRPNNAENDVGNFAAVGTFDPQIEIWNLDCIDKAFPDIILGEPSNHPSLKKKKNKKSKHITTHHIDAILSLSHNKYHRNILASTSADSTIKLWDLNTASAARSLNQIHQGKNVSSSQWHQTESSILLTGGYDSRVAISDVRIAQDSEMSKYWNALGDVENVKWINETQFLAGNDQGGVYCFDIRQESKPLWTLQAHDAGISSLEVNPYVSEMLVTAAMGEKVVKLWKMPTEEGKGPSMVLSRDFGVGNVLTTSFAPDLEVAGNLVVGGVNKGLKMFDFFSNKAVRTNFKDQLKAVQQKARDEAKAQGKVDSRIARKYQGDYSETMLQVEDDDGEDEDDEDEEVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0P1</accession>
    <name>A0A1E3P0P1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">triacylglycerol lipase</fullName>
            <ecNumber evidence="1">3.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_79501</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.1.3" evidence="1"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038162.1">
        <property type="nucleotide sequence ID" value="XM_019184905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0P1"/>
    <dbReference type="GeneID" id="30202151"/>
    <dbReference type="OrthoDB" id="406844at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051299">
        <property type="entry name" value="AB_hydrolase_lip/est"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46640:SF3">
        <property type="entry name" value="LIPASE LIH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46640">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G06510)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133594" description="triacylglycerol lipase" evidence="3">
        <location>
            <begin position="22"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="4">
        <location>
            <begin position="137"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ58955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="366" mass="41652" checksum="D5CEF346EABCD527" modified="2017-01-18" version="1">MFLNRLFSCALVLMISKLVQCHENYHYDNGIYDSLLRYAYISKIAYCIDLLKDGHNLTEPIILKPKPLGFSQEGFASNDYAAQSQEEIVLQVAKLLHAPSTSHNQKAHTTSDDKNDGISESPGYFAIDHSGSGTIILSLRGSVNLRDYFTDLNTRTFDYEPVSERAKLNFTECEGCKVHQGFYKRFIDLEPQIFPTVEKLMEVFPDYKLLVTGHSMGGSLAILAGLEFALMGYDPLIVAYGNPKVSNEPLSDYMDSLFLTDLIEKTIQEDRELTSGCIRVVHNGDLVPMFPPGRKKFVQSGLEFFINKEDLPHPKKSVEYWGKADTITHDSSYKVQDLFSFNMDKIFHLTQHREYFYNVVQCTDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P176</accession>
    <name>A0A1E3P176_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 11">Cystathionine beta-synthase</fullName>
            <ecNumber evidence="4 11">4.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_94093</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>L-homocysteine + L-serine = L,L-cystathionine + H2O</text>
            <dbReference type="Rhea" id="RHEA:10112"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="4.2.1.22"/>
        </reaction>
    </comment>
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        <cofactor evidence="1 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.22" evidence="4 11"/>
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        <property type="reaction ID" value="UER00201"/>
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        <property type="term" value="F:cystathionine beta-synthase activity"/>
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    </dbReference>
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        <property type="term" value="P:cysteine biosynthetic process via cystathionine"/>
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    </dbReference>
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        <property type="term" value="P:transsulfuration"/>
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        <property type="term" value="P:traversing start control point of mitotic cell cycle"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
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        <property type="entry name" value="Cysta_beta_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
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        <property type="entry name" value="cysta_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 10 11" id="KW-0129">CBS domain</keyword>
    <keyword evidence="11" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="357"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005003"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012041"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00026192"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ59155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="495" mass="54501" checksum="8831103226F1B356" modified="2017-01-18" version="1">MTVDRDSKIPELKDSVLELIGNTPLVKLNKIPQSLGVKPQILAKLEFFNAGGSVKDRIALRMVEEAEKDGRIKPGYTLIEPTSGNTGIGLALLGAVKGYRTIITLPEKMSNEKVSVLKALGAEIVRTPTEAAFDSPESHIGVAKKLNKEIKNSVILDQYGNINNPDSHYYTTGYEIYKQSKGKITALVAGAGTGGTITGIAKYLKEQDPKIKVIGADPKGSILAVPESLNKTDTDQYKVEGIGYDFIPDVLNRGLIDEWYKTEDQESFTLARRLIKEEGILIGGSSGSSLAAAIRYAKDHPELTEDDTIVVVFPDSVRSYLTKFIDDEWMKSNGFEIEPVAKSSKGTFETEKIKSLNLKPVVAVHSDEKVEKVIKILKENGFDQLPVLSPKSGKLIGLITLSKLLGSLSSSQITINDAIESIIIDFRKLNNFDDVNIFNENKSSKKKFNKITEETTLGELKKFFEKNASAVVTDKDMKPIHVVTKVDLLDYFIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1D8</accession>
    <name>A0A1E3P1D8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_63649</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183591.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3P1D8"/>
    <dbReference type="GeneID" id="30200837"/>
    <dbReference type="OrthoDB" id="361494at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22652">
        <property type="entry name" value="NUCLEOPORIN NUP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22652:SF0">
        <property type="entry name" value="NUCLEOPORIN NUP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="WD40 repeat-like"/>
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    </dbReference>
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        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="276"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="303"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="563" mass="63405" checksum="6D5E00F6E7C05873" modified="2017-01-18" version="1">MDYYKPPVLFRQNAIKKARSDPGFYLQKNNSSEKWLLKDNVQNDILNKTCSLNKIKIASNYWRIPDDDFYLTAVSVNQQNPEQIAIASGKNESNLFIYDMNFDQDVLTHQQTVSLPNIESMEWLDYDKEESSILTGHKNGVAHMVSIPESNSNESARILKRFNHKKHFTNDKYRNSSIKNLDIPNWNKNSFLSLCNENIFLWDLNHRSDLPILKNQHLGIRNFDSSLSNNGIVALCGEFGIALNDLRAPINTPSIFTPKDSANMGFSNNIKWAPYDSNILAASHVDGVVRLWDIRAQSSFAKLKGHNDLVTSIEWSEESSSDLYTGSRDGNIIHWDLDFDEDLSNCCLNEGLDSINFHKNQFLTDDYDIYNIVNQRQCGTLIPAAKDSIIGLTSTNGNILSIDGSSYLGVHRKRGIDSFRVEEGTSAMIIDDMLNEIPFKRQVSNSGSCTLTDSSDEESIFEQSIQVDSAPSTVNNSPVHVKTRSITKLLNNSDHSVNTLVGSPIAEATSGKILKAQRSTSGSTINDDDQGVCKDFTYEKVLPLNRKLAEIINMERTQQMQYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1F3</accession>
    <name>A0A1E3P1F3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Mre11 DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_17931</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the MRE11/RAD32 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038442.1">
        <property type="nucleotide sequence ID" value="XM_019180766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1F3"/>
    <dbReference type="STRING" id="683960.A0A1E3P1F3"/>
    <dbReference type="GeneID" id="30198012"/>
    <dbReference type="OrthoDB" id="30417at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140445">
        <property type="term" value="C:chromosome, telomeric repeat region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030870">
        <property type="term" value="C:Mre11 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051880">
        <property type="term" value="F:G-quadruplex DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030437">
        <property type="term" value="P:ascospore formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010791">
        <property type="term" value="P:DNA double-strand break processing involved in repair via synthesis-dependent strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990918">
        <property type="term" value="P:double-strand break repair involved in meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035753">
        <property type="term" value="P:maintenance of DNA trinucleotide repeats"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010780">
        <property type="term" value="P:meiotic DNA double-strand break formation involved in reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097552">
        <property type="term" value="P:mitochondrial double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062176">
        <property type="term" value="P:R-loop processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000011">
        <property type="entry name" value="Double-strand break repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.110">
        <property type="entry name" value="Mre11, capping domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003701">
        <property type="entry name" value="Mre11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038487">
        <property type="entry name" value="Mre11_capping_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007281">
        <property type="entry name" value="Mre11_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00583">
        <property type="entry name" value="mre11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139:SF1">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04152">
        <property type="entry name" value="Mre11_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01347">
        <property type="entry name" value="Mre11_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 16" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 16" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11 16" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 16" id="KW-0464">Manganese</keyword>
    <keyword evidence="15 16" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 16" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mre11 DNA-binding" evidence="17">
        <location>
            <begin position="294"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003447"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM01347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ59235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="509" mass="57489" checksum="76911961EA59767C" modified="2017-01-18" version="1" fragment="single">MPQVGPIEPGPDTIRILITTDNHVGYNENDPVRGDDAWKTFEEIMYIARDNDVDMVLQSGDLFHINKPTKKSLYHVMKILRRTCFGDRPCELELLSDPSLVFDDGFNSVNYEDPNLNISIPVFSISGNHDDASGQGLLSPLDLLNVSGLINHFGRVTQSDDINITPLLLQKGLTKLSLFGLASVRDERLFKTFRDGKVKFSLPSIQTDEWFNLMCVHQNHTAHTNTAYLPEHFLPSFLDLVIWGHEHECIPYTVPNAATGFETLQPGSSVATSLCEGEAVEKKVFILNVNQKNYNLEPIKLKTVRPFHMLEISLSTVPAIRPGLAYKNEVTQFLIEKVENLIKEANDDYKLENPDDESPPLPLIRLRVDYSGGYEVENPRRFSNRFVGKVANVNSVVQFHKKDKNLNGLNSKIGKISLSRENGDDGEGAEGIHVETLINNFLKEAELSLLPEQGLNDAIRQFVDKEDKSALKEFVDTEMASEVKQFMQSNDLNFDDDFKTILKKIKKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1G4</accession>
    <name>A0A1E3P1G4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein yippee-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_32714</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the yippee family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038498.1">
        <property type="nucleotide sequence ID" value="XM_019182001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1G4"/>
    <dbReference type="STRING" id="683960.A0A1E3P1G4"/>
    <dbReference type="GeneID" id="30199247"/>
    <dbReference type="OrthoDB" id="6407410at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034751">
        <property type="entry name" value="Yippee"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004910">
        <property type="entry name" value="Yippee/Mis18/Cereblon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039058">
        <property type="entry name" value="Yippee_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13848">
        <property type="entry name" value="PROTEIN YIPPEE-LIKE CG15309-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03226">
        <property type="entry name" value="Yippee-Mis18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51792">
        <property type="entry name" value="YIPPEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Yippee" evidence="5">
        <location>
            <begin position="17"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU110713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="123" mass="14050" checksum="B299535721BBB84C" modified="2017-01-18" version="1">MGLYYQDNLDDFGHRRTIFKCSDCYSHFFTSSDIISDNFHGKNGTAYLVEKVVNVKRGPTEIRSMMTGEYTVCDTLCQQCGKYIGWEYLTADEISEKYKEGKHVIEVCGLGASGMDKKKELLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1H7</accession>
    <name>A0A1E3P1H7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_63600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1H7"/>
    <dbReference type="GeneID" id="30200823"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="101" mass="11232" checksum="CDF50F3E678C9F8F" modified="2017-01-18" version="1" fragment="single">RFNSPPPPPPPPPATAPSEDVPITSDGEHVAESFQQLNLDHGQSSTAQDTSTPSNPDEISLKDQNQMVISYIQQASLHIREAFTSDEMHKYYRIKDNTKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1K8</accession>
    <name>A0A1E3P1K8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_94696</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038576.1">
        <property type="nucleotide sequence ID" value="XM_019186458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1K8"/>
    <dbReference type="STRING" id="683960.A0A1E3P1K8"/>
    <dbReference type="GeneID" id="30203704"/>
    <dbReference type="OrthoDB" id="20669at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991:SF12">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="153"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="294" mass="32622" checksum="790C7D1335931726" modified="2017-01-18" version="1">MSLPTLHSVIKKLNPFSGEISSVLSLEDDPIERVSTLECNDELVIAGSLSGSYYVKNTETQELTKRVLTTNSNGIINFAKAIGDSVLFSTNDSHLITTDLTSLKVKSDVEFPWAINSVSVDPQNQHLKLIVGDNINSFILDDRVHRYKPVNILSGHKDFSFTCDWSHNGMTIATGNQDSTMRLYDIRNLRKNTSCVNGEIESSIRNVKFNYNDTVIAFSESIDYVNILDLKSIDEGTGSKQVISLFGKVIGLDFTNYDDGNGELLTIGVCDDAIGGILQYELESDFKCTDYDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1V1</accession>
    <name>A0A1E3P1V1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Man(5)GlcNAc(2)-PP-dolichol translocation protein RFT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_93589</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Intramembrane glycolipid transporter that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. RFT1 could mediate the translocation of the cytosolically oriented intermediate DolPP-GlcNAc2Man5, produced by ALG11, into the ER lumen where dolichol-linked oligosaccharides assembly continues. However, the intramembrane lipid transporter activity could not be confirmed in vitro.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RFT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038094.1">
        <property type="nucleotide sequence ID" value="XM_019186421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1V1"/>
    <dbReference type="GeneID" id="30203667"/>
    <dbReference type="OrthoDB" id="9979195at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007594">
        <property type="entry name" value="RFT1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04506">
        <property type="entry name" value="Rft-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="61" mass="6906" checksum="71CF777DF432A405" modified="2017-01-18" version="1" fragment="single">MSKPSKDNQDTEKLLSKSAKGVTFLLFGQVFTKFSTFILNQILISYISPKIFGINAFLEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P226</accession>
    <name>A0A1E3P226_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Copper-fist domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_63796</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038507.1">
        <property type="nucleotide sequence ID" value="XM_019183614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P226"/>
    <dbReference type="STRING" id="683960.A0A1E3P226"/>
    <dbReference type="GeneID" id="30200860"/>
    <dbReference type="OrthoDB" id="5600085at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.430.10:FF:000001">
        <property type="entry name" value="Copper fist DNA-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.430.10">
        <property type="entry name" value="Copper fist DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051763">
        <property type="entry name" value="Copper_Homeo_Regul"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001083">
        <property type="entry name" value="Cu_fist_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036395">
        <property type="entry name" value="Cu_fist_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28088">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28088:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00649">
        <property type="entry name" value="Copper-fist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00617">
        <property type="entry name" value="COPPERFIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM01090">
        <property type="entry name" value="Copper-fist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00412">
        <property type="entry name" value="Cu_FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57879">
        <property type="entry name" value="Zinc domain conserved in yeast copper-regulated transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50073">
        <property type="entry name" value="COPPER_FIST_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Copper-fist" evidence="9">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="65"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="336"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="416"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="122"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="338"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="426"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="543" mass="60415" checksum="4105CF5167F7973D" modified="2017-01-18" version="1">MILVDGEKYSCVQCIRGHRSSTCKHSARPLVQVRSRGRPSLNAGHRIAVTESELVLSRNQVTIQDSLNTKSRKSSPNPNPIKESIKKPDEKKSSCCSSKNNTNNTIKNPQPTQAASSCCASKNKSPPTVENNQQQQDQQPGKPCCSTTHSECGCGKNGVIMLRASKRQFVDVRNGNIDYVGPYNESSLNKFKIGMSNTTQPPAKKAKHIRNSSCCSAASASASNKLKVLKQIKMNEFDYQIPRHSSNFEFPGQSNQMPNQPPHLYQYPQQQYQPYQQQAYPPPQNHISRDHLAIGLPFSMTDRPQQGQEYQQYQQQVRPIQSHFHSRSDFDQAFQEYQSQSRAATTQPTTTTISSEPQQDHYQYPQPLQPQPQQKIYDVVYSSGCADECSCGPDCTCPGCLIHRTNEELKEYGILDTSTSSTPSEGPLPVPAPPNSNQPHVGYDLINLETIDEMLLREMFEEECYCKPDECCCYNCSKHGISEGIRLSDGVRVAEADGELDFGKDRSVTPNSHQSGPVSACSNWKSEACKLQGPDIPENGAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P262</accession>
    <name>A0A1E3P262_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Signal recognition particle 54 kDa protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_31559</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Signal-recognition-particle (SRP) assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum (ER) membrane. SRP is required for the cotranslational protein translocation for ER import and preferentially recognizes strongly hydrophobic signal sequences. It is involved in targeting the nascent chain-ribosome (RNC) complex to the ER and is proposed to participate in the arrest of nascent chain elongation during membrane targeting. SRP54 binds to the signal sequence of presecretory protein when they emerge from the ribosomes. SRP54 interacts with the scR1 RNA and mediates the association of the resulting SRP-RNC complex with the signal recognition particle receptor (SR) via its alpha subunit SRP101. Both, SRP54 and SRP101, are locked in their GTP bound forms in the SRP-RNC-SR complex, which dissociates upon transferring the signal sequence to the protein-conducting channel (translocon). After signal sequence transfer, SRP54 and SRP101 act as reciprocal GTPase-activating proteins (GAPs), thereby resolving their association.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Fungal signal recognition particle consists of a 7S RNA molecule (scR1) and at least six protein subunits: srp72, srp68, srp54, sec65, srp21 and srp14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The M domain binds the 7SL RNA and the signal sequence of presecretory proteins.</text>
    </comment>
    <comment type="domain">
        <text evidence="13">The NG domain, also named G domain, is a special guanosine triphosphatase (GTPase) domain, which binds GTP and forms a guanosine 5'-triphosphate (GTP)-dependent complex with a homologous NG domain in the SRP receptor subunit srp101. The two NG domains undergo cooperative rearrangements upon their assembly, which culminate in the reciprocal activation of the GTPase activity of one another. SRP receptor compaction upon binding with cargo-loaded SRP and GTPase rearrangement drive SRP-mediated cotranslational protein translocation into the ER.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038547.1">
        <property type="nucleotide sequence ID" value="XM_019181848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P262"/>
    <dbReference type="STRING" id="683960.A0A1E3P262"/>
    <dbReference type="GeneID" id="30199094"/>
    <dbReference type="OrthoDB" id="10250817at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17875">
        <property type="entry name" value="SRP54_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000022">
        <property type="entry name" value="Signal recognition particle 54 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.140:FF:000001">
        <property type="entry name" value="Signal recognition particle GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="entry name" value="Signal recognition particle, SRP54 subunit, M-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006325">
        <property type="entry name" value="SRP54_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01425">
        <property type="entry name" value="SRP54_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 54K PROTEIN SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF5">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="Signal peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10 13" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="14">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ59340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="528" mass="57518" checksum="DB0C545DC7A50C8A" modified="2017-01-18" version="1">MVLADLGRRINSAVSGAIKSNDVNEDAIDLMLKEICNALLESDVNIKLVANLRKDIKSKLNDDGSKPGVNKKKLIQKVVFDELCSLVDTETEPYKPKKGKTNVIMFVGLQGAGKTTSCTKLAVYYQRRGFKVGLVCADTFRAGAFDQLKQNATKARIPYYGSYTETNPVKVSKDGVDKFKKEKFDIIIVDTSGRHRQEEDLFQEMIEIGDAVQPNQTIMVLDASIGQAAESQSKAFKESSNFGSIILTKLDGHAKGGGAISAVAATNTPIVFIGTGEHVQDFETFSPRSFISKLLGIGDIQGLMEHVQSLDLDNKDTMKNIQEGKFTLNDFQTQMQNIMKMGPLSQIASMIPGMSQMMGQDGEAEASSRLKRMVYIMDSMTKKELDSDGQIFVDEPSRIIRVARGSGTSSTEVEMILFQQRMMATMAQRTKGMLDAKKGGAGGLPGMGPGGFKPSQQQIQQSMQQLQQNPGMMNRLMSMMGGGAPGAGGMPDMNEMMKMMGGAGGMPDISQAMNNPQMQQMMKQFGMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P285</accession>
    <name>A0A1E3P285_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_23585</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038521.1">
        <property type="nucleotide sequence ID" value="XM_019180996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P285"/>
    <dbReference type="STRING" id="683960.A0A1E3P285"/>
    <dbReference type="GeneID" id="30198242"/>
    <dbReference type="OrthoDB" id="415358at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF100">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="2">
        <location>
            <begin position="161"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="441" mass="50510" checksum="B5461EBE0E4D503A" modified="2017-01-18" version="1" fragment="single">EEFFKEYVQKRKPVIIDGVVPGLDISNFQPGSIVDTLSYDKPLLVEKKHKGGFGSGTQRIKMKFDELMGKLKQGDDYYLTTQYEENDPDVEKNIEISDSEEDDEGEEEYDADTLDLNNGADFSDTDSIDMNDLHDDFDDMSESDDDEAEETIRRVKELVQKPLTNLIKTRKLPLRPKILENLAPQQINLWMGSSSSKTPKEKSINIDPKAPDLGLGRQIFGGGVSSGLHHDHSDNLYVPIKGSKRFTIFAPSDGQNLYTVGDIRKIYKTGVIDYKPNEKAPLWRPVRDDGAIVTEAYKWAKINHPKFEQIYDVEQMEQEVEDEDEQLNRAVPDVGLDPPSFSKIPAAVVHLDKIKDDKLREKLTKLAQKRWPKFFNCQSLTTDLKPGQMLYLPSGWFHEVSSFGDLKSDNIHIALNYWYSPPNGDKKAYKDKYWKDDYKRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2E4</accession>
    <name>A0A1E3P2E4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_84950</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038581.1">
        <property type="nucleotide sequence ID" value="XM_019185812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2E4"/>
    <dbReference type="STRING" id="683960.A0A1E3P2E4"/>
    <dbReference type="GeneID" id="30203058"/>
    <dbReference type="OrthoDB" id="60843at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904775">
        <property type="term" value="P:positive regulation of ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000093">
        <property type="entry name" value="Type 2C protein Phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039123">
        <property type="entry name" value="PPTC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320:SF1">
        <property type="entry name" value="PROTEIN PHOSPHATASE PTC7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07228">
        <property type="entry name" value="SpoIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="62"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="322" mass="36216" checksum="B1F32E10F132E107" modified="2017-01-18" version="1">MFCRPLRRSAINLKPRSVKSSSRRFSTSSFSANPSSTGSDIPYKYNIAVAYQPKERDENKFVNQFFKFKKVDSPTGEDNYFIAQRSQDQVAVGVADGVGGWAELGYDSSAISRELCKAIEKGYLQAKDQDGSSYPQYLLDEAFKTIKKEQIVKVGGTTACLGAFQPDGILRVANLGDSWCGIFRENKLIAETKVQTHGFNTPYQLAIIPKELLEKQDNRFIMDKPLDADEYEFKVMQNDIVLFATDGVIDNIDIKDIELFLKDNQDKDLSTISKTFVKEVNKLSVDENFPSVFSQELSKLTGQFYTGGKEDDITVVFVKVDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2H7</accession>
    <name>A0A1E3P2H7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Histone acetyltransferase ESA1</fullName>
            <ecNumber evidence="5">2.3.1.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Histone acetyltransferase esa1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17 26">protein 2-hydroxyisobutyryltransferase ESA1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19 27">protein acetyltransferase ESA1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18 28">protein crotonyltransferase ESA1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="ORF">WICANDRAFT_78325</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="33">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Catalytic component of the NuA4 histone acetyltransferase (HAT) complex which is involved in epigenetic transcriptional activation of selected genes principally by acetylation of nucleosomal histones H4, H3, H2B, H2A and H2A variant H2A.Z. Acetylates histone H4 to form H4K5ac, H4K8ac, H4K12ac and H4K16ac, histone H3 to form H3K14ac, and histone H2A to form H2AK4ac and H2AK7ac. The NuA4 complex is involved in the DNA damage response and is required for chromosome segregation. The NuA4 complex plays a direct role in repair of DNA double-strand breaks (DSBs) through homologous recombination. Recruitment to promoters depends on H3K4me. Also acetylates non-histone proteins. In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as 2-hydroxyisobutanoyl-CoA (2-hydroxyisobutyryl-CoA) or (2E)-butenoyl-CoA (crotonyl-CoA), and is able to mediate protein 2-hydroxyisobutyrylation and crotonylation, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(2E)-butenoyl-CoA + L-lysyl-[protein] = N(6)-(2E)-butenoyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53908"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:137954"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:53909"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>2-hydroxyisobutanoyl-CoA + L-lysyl-[protein] = N(6)-(2-hydroxyisobutanoyl)-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24180"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:15921"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:131780"/>
            <dbReference type="ChEBI" id="CHEBI:144968"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:24181"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-L-lysyl-[histone] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21992"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:21993"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:45949"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="5"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038893.1">
        <property type="nucleotide sequence ID" value="XM_019184702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2H7"/>
    <dbReference type="STRING" id="683960.A0A1E3P2H7"/>
    <dbReference type="GeneID" id="30201948"/>
    <dbReference type="OrthoDB" id="787137at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005721">
        <property type="term" value="C:pericentric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044016">
        <property type="term" value="F:histone H3K4 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010485">
        <property type="term" value="F:histone H4 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106226">
        <property type="term" value="F:peptide 2-hydroxyisobutyryltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140065">
        <property type="term" value="F:peptide butyryltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140064">
        <property type="term" value="F:peptide crotonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140861">
        <property type="term" value="P:DNA repair-dependent chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031453">
        <property type="term" value="P:positive regulation of heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000526">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000013">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.60:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000002">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025995">
        <property type="entry name" value="Tudor-knot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040706">
        <property type="entry name" value="Zf-MYST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF218">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE ESA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11717">
        <property type="entry name" value="Tudor-knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17772">
        <property type="entry name" value="zf-MYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-0010">Activator</keyword>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MYST-type HAT" evidence="31">
        <location>
            <begin position="174"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="84"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="30">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="29">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00045805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00074445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00075313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00076782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00077673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PIRSR" id="PIRSR602717-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="EMBL" id="ODQ59686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="458" mass="53239" checksum="9DF08BABE84B87C6" modified="2017-01-18" version="1">MGISTPDEEGTPAEQVKGESKICTVDDLVLGCKCFVEKDGEHRLAELLSIHVKKGRKVFYVHYDEFNKRLDEWIELDRIDFSRPVTFPKPDTPKKEKQPTKKQATSKNKKKKKDIKEQSATPATPSSVPPQGDDVMDLDNLNVQGLRTEEISREDEIEKLRTGGSMTQSSGEVARVRNLSKVIMGNHEIEPWYFSPYPVELTEEDVIYIDDFTLQFFGSKKQFERFRSKCTLRHPPGNEIYRDDYVSFFEIDGRKQRTWCRNVCLFSKLFLDHKTLYYDVDPFLFYCMTRRDEQGHHLVGYFSKEKESAEGYNLACILTLPQYQRMGYGRLLIEFSYELSKKEGKVGSPEKPLSDLGLLSYRAYWADTIIKLLMENDSDHITIDEISSRTSMTTTDILHTAQTLSMLKYFKGQHVIYLSQEVEAQYLKTEKKKRRSINPELLIWKPPVFTAAQLRFGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2M0</accession>
    <name>A0A1E3P2M0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ELYS-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_91294</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038775.1">
        <property type="nucleotide sequence ID" value="XM_019186281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2M0"/>
    <dbReference type="GeneID" id="30203527"/>
    <dbReference type="OrthoDB" id="3980952at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025151">
        <property type="entry name" value="ELYS_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13934">
        <property type="entry name" value="ELYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELYS-like" evidence="3">
        <location>
            <begin position="61"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="154" mass="18044" checksum="86A976DC0BC98E51" modified="2017-01-18" version="1" fragment="single">MSAELSLFKRLPFPYAKELKDLRNESRPNLYFENVNQSLYAAIKYIRNELVFKKLKDRQKLVLDRYISDIAGLPRLNVDHYYPPENIDKMDKFLTKLKQSGISQVHYVSIVYYILKDFSFDAAESYIKNGASKELSQLGNQSLSCRISKNQYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2W6</accession>
    <name>A0A1E3P2W6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA polymerase epsilon subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">DNA polymerase II subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_52861</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase epsilon subunit B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038751.1">
        <property type="nucleotide sequence ID" value="XM_019182701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2W6"/>
    <dbReference type="STRING" id="683960.A0A1E3P2W6"/>
    <dbReference type="GeneID" id="30199947"/>
    <dbReference type="OrthoDB" id="10254730at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140529">
        <property type="term" value="P:CMG complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045005">
        <property type="term" value="P:DNA-templated DNA replication maintenance of fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016266">
        <property type="entry name" value="POLE2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12708:SF0">
        <property type="entry name" value="DNA POLYMERASE EPSILON SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12708">
        <property type="entry name" value="DNA POLYMERASE EPSILON SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="9">
        <location>
            <begin position="362"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="514"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="514"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="637" mass="72491" checksum="EB573AC697758F7B" modified="2017-01-18" version="1">MDHQPIVLPIRLQPSNLRPIAYRILSKKHGLNIKSDALKILADYIGPKFGNEWKSAKSQHFLEEIARIWKEQDRGLFIDGEGLGEVMKEIDSLNTKVAKKMNPSSTTHQPVQTSVDALMAGRRDTIVDEITISEDEVSRTSPPTSVSPPPIRESLNWKDYFKVINAFDQPLFDYNHHRKQYELSKTKDSKFTSTSVDANISLFQRRFHIVKDRLLRNENFQASSFNAFSSIQGDSENNSITQIKNLLGRNNQRFMLFGMLTLINGDWHLQDHSDKIQLDISQAEPNLGSYYVPGNMVLVDGIHASGKFYCSSMGHPPGERRIDTLEANGNIDFLGIHANLRIDKELNSKLKLLEREMDQKIIILGSNCYLDDLKTLDALSKLFNKLSEEPNDLPISIVFNGSFASSPKHSSEYKNLFDSLAAILDTFPVISGNVQLVFIPGENDQWQTPLWPKQTIPKIFGNRINRVARNVQWASNPTRMVYLSQEIVIARDDLGSRLRRNTVLFPKLNKKLDDEDEDLDEGEDDNGDESRTEIERLESLPPRISEARKVVKTILDQGHLSPFAKNLKPIIWHLDHTLHLSPIPSVLILSDPSSPAFDVTYNGCRCLNPGILIQKRKLNYIEYSLSTRKAEIKELHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3D2</accession>
    <name>A0A1E3P3D2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">General transcription and DNA repair factor IIH subunit TFB4</fullName>
            <shortName evidence="14">TFIIH subunit TFB4</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 14">RNA polymerase II transcription factor B subunit 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_31959</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 14">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the 7-subunit TFIIH core complex composed of XPB/SSL2, XPD/RAD3, SSL1, TFB1, TFB2, TFB4 and TFB5, which is active in NER. The core complex associates with the 3-subunit CTD-kinase module TFIIK composed of CCL1, KIN28 and TFB3 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the TFB4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038609.1">
        <property type="nucleotide sequence ID" value="XM_019181903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3D2"/>
    <dbReference type="STRING" id="683960.A0A1E3P3D2"/>
    <dbReference type="GeneID" id="30199149"/>
    <dbReference type="OrthoDB" id="17307at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000093">
        <property type="entry name" value="Transcription initiation factor TFIIH subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004600">
        <property type="entry name" value="TFIIH_Tfb4/GTF2H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH TFIIH , POLYPEPTIDE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03850">
        <property type="entry name" value="Tfb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="83"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="335"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="348"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ59402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="364" mass="40274" checksum="1922A4F6ACA66A3A" modified="2017-01-18" version="1">MDAIADKTFATEAGSKNVTEESPSLLTIILDTNPVVWHKISDKLSLKSMLKSLVIAMNAHLALNNSNQIAVVASHSDGARFLYPSTKTEDQETSKPKTRSEHIIAEEDNNGYESRSISPEAEKSSTKFVNSSMYRQFKVVDESFLENLYQLLNEPIPTKPPKNSLSSSLSLALTYINKVQTMNSLMKARVLVVNISQDEQLKYIPIMNCIFAAQKMKVSIDVCQLGTDATFLQQASDATNGVYLHVENLDGLIQYFTTALFIDPSIKNILVRPTSGNIDFRASCFLTGKVVDIGYVCSVCLCILSLIPENNKCPACDSEFDNHVIMKLKRRPVVVKSKKKKRKLDNGNSSTSTPKPSNTQAPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3P7</accession>
    <name>A0A1E3P3P7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_84072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039323.1">
        <property type="nucleotide sequence ID" value="XM_019185642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3P7"/>
    <dbReference type="STRING" id="683960.A0A1E3P3P7"/>
    <dbReference type="GeneID" id="30202888"/>
    <dbReference type="OrthoDB" id="435593at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061015">
        <property type="term" value="P:snRNA import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035719">
        <property type="term" value="P:tRNA import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000266">
        <property type="entry name" value="mRNA transport regulator MTR10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051345">
        <property type="entry name" value="Importin_beta-like_NTR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057941">
        <property type="entry name" value="TPR_TNPO3_IPO13_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057942">
        <property type="entry name" value="TPR_TNPO3_IPO13_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058537">
        <property type="entry name" value="TPR_TNPO3_IPO13_4th"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363:SF53">
        <property type="entry name" value="MRNA TRANSPORT REGULATOR MTR10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363">
        <property type="entry name" value="TRANSPORTIN 3 AND IMPORTIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24138">
        <property type="entry name" value="TPR_TNPO3_IPO13_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24140">
        <property type="entry name" value="TPR_TNPO3_IPO13_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24139">
        <property type="entry name" value="TPR_TNPO3_IPO13_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="1">
        <location>
            <begin position="11"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="918" mass="105185" checksum="3080F2AE4997C578" modified="2017-01-18" version="1">MSSNNSDKTSALHFLEEFQKTPEAWQVVHTILGDGQYTVEMKMFAAQTLRNKLTYDLHQVPEDSLSGLKDSIIQFLIQYSESNKPIRTQLSIALAKLSIQYLTWPNAVDEISSKLSQHIPTLLEFLKILPEESLDPKGTPLTDEEFRLRTEELIINNVERVLLLLSNYASSTSDSKASSLILDCLNSWIREIPVEQLLTIDPLTTIIFQSLRDEESFDKAIECLVSIVRETRDIENIQLIQALFEQIIQLRPLLQQNVDDVDVFGSLTRLFVESGESWHMLIAKAPKDFRPLVEILLECTAYEEDLDIVKYTFYFWYNLKQVVTIDRFKPAREEFLNIYTQLVHIMIKHLHYPDGSETDPLFSSKEEEEKFKDFRYDMGDVLKDCTAVIGASKALTIPYEQIRSSLNSSNVKWQDVEAPLFSLRAMAKEVSLKEKEVLPQIMELLVQLPENVKIRYAATLVLGRYTEWTAKHPEQLEKQLNYIINGFQAADADIITAASHALMYFCQDCSKLLTNYIEQLYNFYINIFGSPMVDLTSFYEITEGIAHIINAQDYSNIASATTMFIKPVLEKINGYVNAEGSEEVYKAIAEQIEIVRIFLEFIKPRDFHTPEDPVANLVIEMWPMVIGLLDHHGHSMKVSERCMKFTKTVLQTYNVFLTPILPSVANALVKGFQETRYGCYLWVSGIVIREFGDEYSSAEIKESVWNFAYQQIGTFLVVFEQAKPIDIPDLIEDFFRMMSDIIMFFVTNFVLSDILGSTFEVALLALDIEKFEPLIATLHFLIDLVSWGFDVPPISIYENVPEDVKLTVQRFVGQHGDSLLKHVLHGLIFKFPADAHPDASDLLGKTLRLSPSPQTAVNWINIALDALPQNTVSDQERNKLVTTISSALVSKDNRRIRTSLKDFVTWYSRKNITPRFQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3T4</accession>
    <name>A0A1E3P3T4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Adenosine kinase</fullName>
            <shortName evidence="11">AK</shortName>
            <ecNumber evidence="4 11">2.7.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Adenosine 5'-phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_84100</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>adenosine + ATP = AMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20824"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.20" evidence="4 11"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039358.1">
        <property type="nucleotide sequence ID" value="XM_019185648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3T4"/>
    <dbReference type="STRING" id="683960.A0A1E3P3T4"/>
    <dbReference type="GeneID" id="30202894"/>
    <dbReference type="OrthoDB" id="432447at2759"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00659"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004001">
        <property type="term" value="F:adenosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01168">
        <property type="entry name" value="adenosine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000014">
        <property type="entry name" value="ADO1p Adenosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001805">
        <property type="entry name" value="Adenokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769:SF3">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00989">
        <property type="entry name" value="ADENOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 11" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="12">
        <location>
            <begin position="24"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601805-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ60151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="348" mass="38005" checksum="90543BCE30CDF18D" modified="2017-01-18" version="1">MSYPLVALGNPLLDFQVNVEPSYLEKYELKANDAILVEEKHLPIFGECINDPSVQFVAGGAAQNAARGAAYILPENSVAYFGSVGKDKYSELLLEANAKAGVKSLYQFQTEHETGKCAALITGHNRSLATDLAAANHFTPDHLKKAENWEYVEKAKVFYIGGFHLTVSPEAIYTLGQHAAETNKVFSLNLSAPFIPEFFKDVLDKSVTFADYVIGNETEAEAYARSHNLETTDLKEIAKYIAKEPKVNEKQNRTVVITHGLEPTITVTYNKSADSFDIKEFPVHPLDSSKIEDTNGAGDAFAGGFLAGLVNGDDLASSIDKGQWLAKLSIQEVGPSFPYPKQPYTSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P426</accession>
    <name>A0A1E3P426_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_30061</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039446.1">
        <property type="nucleotide sequence ID" value="XM_019181682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P426"/>
    <dbReference type="STRING" id="683960.A0A1E3P426"/>
    <dbReference type="GeneID" id="30198928"/>
    <dbReference type="OrthoDB" id="5584247at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07440">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052246">
        <property type="entry name" value="Cell_Polariz_PKAAnc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13155:SF1">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13155">
        <property type="entry name" value="A-KINASE ANCHOR PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="3">
        <location>
            <begin position="210"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="406" mass="47346" checksum="3F2FD35330A92705" modified="2017-01-18" version="1">MSSPFKPSAKQTLLPNSNINNLLTDTHSIPSSFGLESNHTTTPYHHERPTLNRLPTLYEVLNRRTEPPVDLWSFYIYMRDYQNSVDYLDFWIDVITHLRLCKDYVKGLRESLLLSEKHKSSSDEKKRHTTTSSTDEGNGGAEQRASVSSSLLLEALLNEGFLDDKDNKRHKSGSIQEQRNSTRILPEQLERYTQNIKNGNISRTTLKSSSRNILNTYFQDSSPRKLQIPDRIIRKIKHDVELQGRDDPEVFDDARNYVYNLMERECFPFFLQKNALHNLNNKSCLIRLILGLFWIFAGFWISYVLIFINIKPKAIRAVIIVPFLIGVYLILSFLYKLDPIMVFAKYSDNQELYQPKNQKLKKWVRNLKEPFVLRLLKKRALWVIFLVLIITAILSILFGLVPGHRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P447</accession>
    <name>A0A1E3P447_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peroxisomal membrane protein PEX25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_92176</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039275.1">
        <property type="nucleotide sequence ID" value="XM_019186350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P447"/>
    <dbReference type="STRING" id="683960.A0A1E3P447"/>
    <dbReference type="GeneID" id="30203596"/>
    <dbReference type="OrthoDB" id="411017at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF50">
        <property type="entry name" value="PEROXIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="1" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="105"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="27814" checksum="A796EA39D4268FDE" modified="2017-01-18" version="1" fragment="single">MLERKIFDITKNISIFRQMLRFGGTPYRIRQFLIKFNKFIKSGPTNLINIYKFWINEDSLGDFIDLYYGICDEIILLYKLNVFTNPNFKSFIGKHEAYSWYMDILLGLKKNYNKLQENRNKQLQLNIQNQVKQKASLLSKRLMDSIGNNSPMKSQILREFNTKSPILNNNNQYDLEIEALKHEERIIMTDLVRLSFDFVCDSIDIFKLELNPSVYLICGAISGSFGLSKVWMMSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4B4</accession>
    <name>A0A1E3P4B4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_30983</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039241.1">
        <property type="nucleotide sequence ID" value="XM_019181784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4B4"/>
    <dbReference type="STRING" id="683960.A0A1E3P4B4"/>
    <dbReference type="GeneID" id="30199030"/>
    <dbReference type="OrthoDB" id="449382at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026893">
        <property type="entry name" value="Tyr/Ser_Pase_IphP-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF1">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13350">
        <property type="entry name" value="Y_phosphatase3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="8"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="584" mass="66482" checksum="78DE6DA6EC6F528F" modified="2017-01-18" version="1">MESPPPTHKLSLILHGKGGHKNYCYQAMLAQELATKLGIYSFRLDFRGCGDSQQNEDESMGRMISQDIEDINLSLDVFSNGASFEEIGINLTTHSIVAHSRGSVAMMRWAIEEQKKLDKGEPNYRFVQNLVNTAGRFNGKLLLDGLKIQASPEGYTLNQYRLGNYQDLVIQTPETADLGSQNLEEMKYINDEIQVLSVYGLHDHIVPIEDSTHFANLLGSRHTLRFINFADHNFYGSQEVTEKNKSRLNPNGYPLNKRNKVNYNYQATEIIIDYLSSANDLERFKTSTDIVYQSPRWREIDGIANFRDIGGWRTKDNKHYVKSGVIFRSANTSNVTETGKKQLNELGIKSVFDFRSYGEFKKAGGLEIKGVNVNNIPVFTKTDVSPQSIALRYKNLLTSWYTFKYVYENMLENGSYAFKSVLLFLRDHENVPIIFNCTAGKDRTGVMAMIILLLLGVENHIIAKEYELTTIGLIPNHAQIKKEFFEASNDFKEKFKDSDHFKEFSSLSPDAMFNNLISSKYESMIATIELFNQKFGGIEKYCIDYLGLSKEDLSKIRSNLLTNSSPYPDQNAIWKHRAQSGSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4C9</accession>
    <name>A0A1E3P4C9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_84180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039492.1">
        <property type="nucleotide sequence ID" value="XM_019185660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4C9"/>
    <dbReference type="STRING" id="683960.A0A1E3P4C9"/>
    <dbReference type="GeneID" id="30202906"/>
    <dbReference type="OrthoDB" id="2307332at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="501" mass="56607" checksum="8E98347DD37C1D77" modified="2017-01-18" version="1">MATGECVSTVNNNAAEANHGGLNTFLTSNDLYKSNSQESSMFNSDDLMFSQQGAYAQMNKSNQLNGLFNNDIYFVNGLLTPPNSDNTEVNQSVSNLNTVNNINNYNNNADINHQVLSFNKEFEDVDYFGSSFDHLPSFNLKKPQLSSPLHSPNYHTTFEEFQPYFTTASANKKLPDHSKLYVKEKPKHFEHSAKKNRNTSVPAPTVSAGGVTKPPANKRSKTLNDLKIPTKLASVSPSPIDFNPAVPNQFQWINVSPITDARYKEVLELAGQRKLDKKKVIKILDSFRQNDVSEKLNLINRKLGSKISSSSSTKLAKMNIREVNGSFQIICNTSSGYDTPVEEHESIPSGLLKLNILKDHVNYKLYDELASSLKVMAEQNSKNDKIKRPLNQFMLYKRSMIKAANIFKVVEMVKENNHDELLKMDEELKRNPKSSLLNEIVDVQPKLDHHNTCHVIALLWVTESEAVKEQFAKFAKLEKDIHGQVFPNYKFNPQKRNSTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4N6</accession>
    <name>A0A1E3P4N6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">THUMP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_62437</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039059.1">
        <property type="nucleotide sequence ID" value="XM_019183365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4N6"/>
    <dbReference type="STRING" id="683960.A0A1E3P4N6"/>
    <dbReference type="GeneID" id="30200611"/>
    <dbReference type="OrthoDB" id="367221at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051391">
        <property type="term" value="P:tRNA acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11717">
        <property type="entry name" value="THUMP_THUMPD1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2300.10:FF:000001">
        <property type="entry name" value="THUMP domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2300.10">
        <property type="entry name" value="THUMP superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040183">
        <property type="entry name" value="THUMPD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452">
        <property type="entry name" value="THUMP DOMAIN CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452:SF10">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="3">
        <location>
            <begin position="132"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="260"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="305" mass="35244" checksum="5C10F061450744E7" modified="2017-01-18" version="1">MGEKRKNNGSNGQDRKKKYKLANGIIDPSTSGIYATCLRHHEKQCQNELKNLFQDKYQEYYKDVEAEDDEEEEEELSIEDAIKKELQELQQPKDTTKKEPFQFIDLGCECVVFFKTRKPVDPVDFVERICQESHEHSIKNTRHTQKLTPITFSVSASMEEVTKLAKKVLAPHFHKEEGQEPVKFAVKVSARNFNTIDKMDIIQKVAECVGRDYGHKVDLKNYDKLVLVECFKNNIGMSVVSNYDKYQKFNLQQIFEKANESKAKKEEVSLKSTEVEEKTSESPVEDQDTKVKKLEDSTEKSAVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4V6</accession>
    <name>A0A1E3P4V6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="4">PPIase</shortName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_12602</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039555.1">
        <property type="nucleotide sequence ID" value="XM_019180536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4V6"/>
    <dbReference type="STRING" id="683960.A0A1E3P4V6"/>
    <dbReference type="GeneID" id="30197782"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000001">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="5">
        <location>
            <begin position="8"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="166" mass="18553" checksum="4C0C3C6FFD2F473E" modified="2017-01-18" version="1" fragment="single">PPITHYVYFDIAHGEEAIGQIKIGLFGSVVPRTVENFFELATKMGEKNRGYNNTIFHRIIKDFMIQGGDFEFGSGIGGYSIYGKKFEDENFKLTHCKIGRLSMANAGPDSNGSQFFITTSITSWLDGKHVVFGQVVEGLDLILEKIQTVKTGQNDRPEKDVRIVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P519</accession>
    <name>A0A1E3P519_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Factor arrest protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_67236</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039732.1">
        <property type="nucleotide sequence ID" value="XM_019184076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P519"/>
    <dbReference type="STRING" id="683960.A0A1E3P519"/>
    <dbReference type="GeneID" id="30201322"/>
    <dbReference type="OrthoDB" id="18234at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000138">
        <property type="term" value="C:Golgi trans cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000321">
        <property type="term" value="P:re-entry into mitotic cell cycle after pheromone arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040185">
        <property type="entry name" value="Far11/STRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021819">
        <property type="entry name" value="Far11/STRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012486">
        <property type="entry name" value="Far11/STRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13239:SF4">
        <property type="entry name" value="AT25231P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13239">
        <property type="entry name" value="PROTEIN REQUIRED FOR HYPHAL ANASTOMOSIS HAM-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11882">
        <property type="entry name" value="DUF3402"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07923">
        <property type="entry name" value="N1221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01293">
        <property type="entry name" value="DUF3402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01292">
        <property type="entry name" value="N1221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Far11/STRP N-terminal" evidence="1">
        <location>
            <begin position="77"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Far11/STRP C-terminal" evidence="2">
        <location>
            <begin position="423"/>
            <end position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="782" mass="89718" checksum="9F0289CD51D5E699" modified="2017-01-18" version="1">MFGTMEIGGTDDGDYDEDDIDQLNIDEVDQARSDVLDNNLTVDEEFQHSLNERANQFSAAGSEGNSINTTSFPSIPKPKVDYQYNDDFSLENELNDWFTANELKKLPLFKQIYMNVINVPDFGLLREYEQSKVVQLLLKEVQKDEPLNVLYCITYISLGAYGAHKNLSHHVESVKKNNRLLSENNITPILIKIITKSFYNAKESKDKGSFHSNILFLSSTILFFLICCELDSKDQCSPVLDQLEEQDFLNTLVIFIDEWRWNSLPNLRIRNIIALFAKTFQFLIGGLNDRSKSKDYTCAQFGISKEQNPSKLTTTPLDYYVFREDILARYPTSIPVPSTLPANFENSSSLSQFINIARTLDTSKSNAALPVPSVHIATPASSPPSTPVNKTNKVERSYQTNQSYPFIYPTVSGETEYDVVNVPKSIEEASQLCASRVQEKLSLKQLWNERDHFMQQERGWTDALNHSDSKFDDYRLLDPKPELKPLIRVENFYSKSIPYLSSLVHVLLQVIISSQENNQLLEDDSKDTLNQMDLELIKAKEVSLKNGSYILVLVSKWLKVSHILKFEYLCTLIYDSNYFAILMQYLKSTGDSLFSRIRNTVLNENRYSFWSQCASLSPSAYSIPKPTCSIDENFCFSLTNLLTIGSLICHNKTQRIIALSEKDPARLFKPFFLIINSSLWKPVLKMVKEITPFNGRKWKSYNMDLISMVYLHMKPQLKENWLSGRDLDGELKDAYGQEIALRAIIQFYNLRRYGDVMSKLGYEKRGSDFFTREMELLAVSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P544</accession>
    <name>A0A1E3P544_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_19526</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ60589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019180828.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3P544"/>
    <dbReference type="GeneID" id="30198074"/>
    <dbReference type="OrthoDB" id="4726at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12306">
        <property type="entry name" value="RRM_II_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF12">
        <property type="entry name" value="EUKARYOTIC INITIATION FACTOR 4B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
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        <location>
            <begin position="7"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="86"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="113"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="126" mass="14052" checksum="8670E1BE59032096" modified="2017-01-18" version="1" fragment="single">QKEIDSRSVYVGNVDYKATPEELDEFFRTIGSINRVTILYDRFTGYPKGYAYVEFESKDSVEPAIELSGTEFKNRQISVVAKRTNLPGFRRGRGGFRGGRGGRGRGRGRGGFRGRGGFRGGRGGFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5B9</accession>
    <name>A0A1E3P5B9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_27938</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019181428.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3P5B9"/>
    <dbReference type="GeneID" id="30198674"/>
    <dbReference type="OrthoDB" id="6354873at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000248">
        <property type="term" value="F:C-5 sterol desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="6">
        <location>
            <begin position="171"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="349" mass="41239" checksum="58AD398912213FFD" modified="2017-01-18" version="1">MDLVLEFVDSHGVDYLYAKLFPASLSSHIPEVLQKSLDLVLSELQESDVYGKIPVFFDTSIYTNASLLPRYNLLRQFLTLFPLMWFFGIALYLTVATFSYVFIFDKAIFNHPRYLKNQMSLEIKQAVNAIPIMVLLTIPWFLIELQGYSKLYWDVDSVKGGWWSIVLQYPAFIMFTDFFIYLIHRWLHWPRVYKALHKPHHKWLVTTPYASHAFHPVDGYFQSLPYHVYPFVFPLHKISYLILFTFVNFWTVMIHDGEYLANDPIVNGAACHTVHHLYFNYNYGQFTTLWDRIGKSYREPDKELFDKSKKKSTKTWEEQVVKMEAIKSVVEGNDDDRVYGTEEKINKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5I9</accession>
    <name>A0A1E3P5I9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_77313</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039840.1">
        <property type="nucleotide sequence ID" value="XM_019184537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5I9"/>
    <dbReference type="GeneID" id="30201783"/>
    <dbReference type="OrthoDB" id="19092at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000283">
        <property type="entry name" value="NAP1-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="110"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="188"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="199"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="223" mass="26104" checksum="C9900F8E647519CA" modified="2017-01-18" version="1">MSIHQDQVDLDKSHPTMISVKDFAYEEDEDLHYGVINYSQYLTDEDINYDENNEHNEHDENDIRNSIINIPMNYYFEDGPPWKEDPDLKTPMIKDDSKNQFEFSIASSDEIHGKAIALFDFIPENDNEVGLKEGQIIWISYRHGQGWLVAEDSKTGETGLVPEEYVQLVNNDIEDYIEDEPRRFLPDIINDHDHGVNHDEEEDSDWVDEDDEELPNVEKLDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5N4</accession>
    <name>A0A1E3P5N4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_82946</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039761.1">
        <property type="nucleotide sequence ID" value="XM_019185465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5N4"/>
    <dbReference type="STRING" id="683960.A0A1E3P5N4"/>
    <dbReference type="GeneID" id="30202711"/>
    <dbReference type="OrthoDB" id="10257314at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000008">
        <property type="entry name" value="Alpha-ketoglutarate-dependent taurine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051323">
        <property type="entry name" value="AtsK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468:SF9">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT TAURINE DIOXYGENASE (AFU_ORTHOLOGUE AFUA_3G01010)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="8">
        <location>
            <begin position="106"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="376"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="398" mass="45171" checksum="C25A8056E3B5A81E" modified="2017-01-18" version="1">MTTISQLSQKTKDLSLQSIQIELKNPNGVQEGYQDFAKELPALSRKRLEKYGIDLSKGYPRRPDHIPIFLDEAYKIRNRPNPNYIERGLNADPEKKALFGAATEVVNLTKYIGTEIVGLQLSDLNEQQLDELALLIAERVVVVFRKQDLSPQKQLEIGEYYGEVEEHPLTGQAKKGITVVWNKFNRGGPLIKFQRGAFAGWHTDLDHEFSPAGITHLHLDSTSETGGDTGFISGYGAFDKLSPSFQKFLEGKVAIHKSAHVYYERDDILSGPKHIEREHPLVVTHPVTGWKSLFVNPAHVVRIKDLEKEESDLVLKYLNEVYAKNLDIQVRVHWQPVKEDEGVSVIWDNRISQHVAISDYNDQWEGPVRHAHRVTSLSTPPVFDPNSKSQREALGWDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5R4</accession>
    <name>A0A1E3P5R4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH-ubiquinone oxidoreductase B15 subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_62741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039384.1">
        <property type="nucleotide sequence ID" value="XM_019183428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5R4"/>
    <dbReference type="GeneID" id="30200674"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39476:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39476">
        <property type="entry name" value="NADH:UBIQUINONE OXIDOREDUCTASE 6.6KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9398" checksum="0C75EF5A93CC2A93" modified="2017-01-18" version="1">MGGHGHGPGPLKIDNAFEQYNKFYGTAQYRFKFTAKNIPSVLLLGLVVPVGLCYLGYQSQGWVERQPKRRTTQIHERDFKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5V1</accession>
    <name>A0A1E3P5V1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_28072</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040028.1">
        <property type="nucleotide sequence ID" value="XM_019181446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5V1"/>
    <dbReference type="STRING" id="683960.A0A1E3P5V1"/>
    <dbReference type="GeneID" id="30198692"/>
    <dbReference type="OrthoDB" id="10251136at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19509">
        <property type="entry name" value="RecA-like_VPS4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000022">
        <property type="entry name" value="Fidgetin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000093">
        <property type="entry name" value="Fidgetin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF17">
        <property type="entry name" value="FIDGETIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="472"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="173"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="224"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="176"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="722" mass="80470" checksum="3ECCB87D14035E91" modified="2017-01-18" version="1" fragment="single">LSLLHSMNFIRKKPSAPLDDLTDLYNETANKTIYNLGLEQKNELKKAVQGWKALHTHVLFKLDLIDKAYSGSNYSADEIYLREGIEDLDRKGLRHLDRVNRAYDDLVNKYEMRQNLPQQNQQQQQPAQSSSRMLKTLRPERPHKFNSNHNYANKPSELSKIAATTMYYQPSKQNWSTSKSLNSSSTSAFNKSHNPDDIFDQSFEDVDFDLKTLKRDDAFSQLEATGTQSQLARSKPNDEVASQFEKLRIDSPSKRVTSGSSKPGFPSVVHSTPNLIDIDGKAQPATKPTTTSTTPRRTIPTTTAKPSATARTTRPGVKAATNPTTTQAAKRAVKPTSTSTTTRPAVKTTKSSTTKPQTTKSSTPRSRSQSPIRGETKDTETISSNEEESDGFADESQLSEKEIREKMEEQIISEIRGIDHGAAKQIFNEIVVHGDEVHWDDIAGLETAKNSLKETVVYPFLRPDLFSGLREPARGMLLFGPPGTGKTMLARAVATESKSTFFSISASSLTSKFLGESEKLVRALFMLAKKLSPSIIFVDEIDSLLSSRNESGEHESSRRIKNEFLIQWSDLTHAAAGKDTGEDLQRVLVLAATNLPWAIDEAARRRFVRRQYIPLPEPETRQAQIIKLLAHQKHTLDEKDQLKLIEMTDGFSGSDITALAKDAAMGPLRSLGDKLLSTARDEIRPINLSDFESSLKYIRPSVSKENLSEFEDWASKYGSSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5V9</accession>
    <name>A0A1E3P5V9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_67484</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040022.1">
        <property type="nucleotide sequence ID" value="XM_019184086.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P5V9"/>
    <dbReference type="GeneID" id="30201332"/>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000388">
        <property type="term" value="P:spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18019">
        <property type="entry name" value="DEXHc_Brr2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18021">
        <property type="entry name" value="DEXHc_Brr2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000133">
        <property type="entry name" value="Pre-mRNA splicing helicase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000004">
        <property type="entry name" value="RNA helicase, activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000102">
        <property type="entry name" value="RNA helicase, activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000024">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000001">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000062">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000254">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000013">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041094">
        <property type="entry name" value="Brr2_helicase_PWI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048863">
        <property type="entry name" value="BRR2_plug"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21188">
        <property type="entry name" value="BRR2_plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18149">
        <property type="entry name" value="Helicase_PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039073">
        <property type="entry name" value="BRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="484"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="702"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="1329"/>
            <end position="1505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="31"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="210"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1792"/>
            <end position="1812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="47"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1792"/>
            <end position="1811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2141" mass="243853" checksum="F732CD3DE4B8624F" modified="2017-01-18" version="1">MSDNNKYKYEEMSNKVLRQDKKLLSNRADPLKDAALSAPTSLAGKVSLKDMGSRSATTKDADGDEEKRVAKKVSDQLRSEELEKRRNIKSNRLNNSTLLDSGSIESLNYYPKSQETNEVYEKLVSWTSNQFGNDIQHNIVISATDSILEVLKDEKLTDRQKKKQIEQDLDLKITEDSFSEAIKLSNDITDYDAQDDNEGDDNEAGVAVDFEEEEEQQEQQESEEESDDDFAGPALDDFVQEEEKEGEEVIKFDQKSSAKAEIIYMKDVDAFYLQRIISSAFPNLDAMGVQSLTSTIYSLIEEQSSDKVFEQKLLENIDSEKIDFVRLLITNRQTILWGTRLSKADNSDERQAILKELESNDLKHLLDEYEESDGTENDRVTKRRKVVSTGDEQISAIRKPKIVNLDDLTFDQGSHLMTTTKVNLPKDSFKRTKKAYEEIHIPAPAKPLDDSELVSISTLPPWARQAFPSAETSTLNRIQSKVFPTAFNDDSNILLCAPTGAGKTNVAMLTILRTLSHFRRDDGSFDLSNFKIVYIAPLKALVQEQVREFSRRLEVFGITVEELTGDSNLTKQQISETQILVTTPEKWDVITRKNSDTSYTNLVSLLIIDEIHLLHDERGPVLESITARTLRNVEYSGRDVRLVGLSATLPNYKDVAKFLRVDESKGLFFFDASYRPCPLAQQFIGITEKNSLKKTIAMNEACYDKILEAAGKHQVIIFVHSRKDTFRTAKWLRDKLLEEDKLNLFLRSDSASIEILRQESEKVKDTGLADLLPTGFAIHHAGLSREDRSASEDLFAEGYAQILVSTATLAWGVNLPAHTVIIKGTEVYAPDKGDWTQLSPQDILQMLGRAGRPRYDESGEGIIITSQSEIQYYLAVLNQQLPIESQLMGKLADNLNAEITLGTVKSLQEGVEWLGYTYLYVRMLKSPDIYRVGPEYKDDVALEWKRADLIHSALTVLHQNNLVIYDSVRGTVQSTELGRISSHYYISYESMSFYNKQLKSHSSVIDIFRIFASSSEFKLIPVRQEEKAEISKLIERAPIPIKEDVNDPRAKANVLLQSYISRLSLDGFALNADMIYITQSAGRLLRAIYEIVLKKSWAGLSKYLLDLCKMVEQRIWLSNSPFRQFPDCPPKVIKNTESSNLPWSEYFNLSDPSEMAQAIRDDASARKAFELINKFPKLTMRCSVQTLTPTLLRFELDVLPEWNWDPRYHNNAESFVLFVEDTNGEKILYYDSFLVRRNYINQEHLIDFTVPIDTPVPPNYFVTLISEKWLHCEYRIPVVFNSLKLPKKFPAPTPLLDLHPVPIDDLKVPDFISSFDFDFFNKFQSQVFNTLYHTDENVFVGIVKGSGKTVIAELALLNHWKQNKGRAVYICPSQEKIDILVKQWSSRFGDIAEGKVINKFSNELSSNLKVLADSHVILSTPQQFDLVSRRWKQRKNVQSIELFIADDVQIVGNGAEGSVYENIISRMRFMSSQLENKLRIVALSTSLANGRDFGEWIGADKNNIFNFSSKERIRPLEIHLQSYNINHNPSLILAMIKPTYLAAKGYEAGSSLVFVPSRKQCIEIGLEFLRLANADETSFLNANIEDIEPLLNKVSDQSLKDSLSSGIGFYYKNMGATDKKVVEKLFNKGALSVLFATKDTAFYAPTANLVIVLSTQFYEGKEHRYIDYPINELLEMIGCSQGENGESDKVMILTNNSKRDYYRKFLNESLPVESFLNVFLHDAFLNEISTGLIKSRQDCIDWLTFSYFYRRLQANPSFYGVKDISHVGLSSYLTELVESTLNELSEAKLIELEENDEDEEEEEEEEEEEISPLDGALVASYHNVSFITMQTFILSLTRKSKLKSILEIITSASEFDSLPIRQHETSILNKIYNRVPIKSSSDANFESPYLKAFILLQAHFSRLNLPPDLASDQRFILEKVLTLLYTAVDLLSSEGYLNAMYAMDLSQMVVQAVWDTDSPLKQIPFVDDATIERASKYKVESVFDIMSIEDDERDDILRLSDRPLNQVAEFVNKYPNIEISYSLDLSEPIVSNDPKRLVVNVTRDEEPEDLEVVAPFYPFSKAENWWVVLGDSQTRQLYAIQKLALSKEEQQVKLDFTIPRAGHHNLSIWCMCDSYVDADKEISFEVDVEQGEEDSEEEVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P602</accession>
    <name>A0A1E3P602_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Translation machinery-associated protein 20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_83041</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 6">Involved in translation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 6">Belongs to the TMA20 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039907.1">
        <property type="nucleotide sequence ID" value="XM_019185480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P602"/>
    <dbReference type="STRING" id="683960.A0A1E3P602"/>
    <dbReference type="GeneID" id="30202726"/>
    <dbReference type="OrthoDB" id="10249667at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11609">
        <property type="entry name" value="MCT1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21155">
        <property type="entry name" value="PUA_MCTS-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.400.20:FF:000001">
        <property type="entry name" value="Malignant T-cell-amplified sequence 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016437">
        <property type="entry name" value="MCT-1/Tma20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041366">
        <property type="entry name" value="Pre-PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798:SF0">
        <property type="entry name" value="MALIGNANT T-CELL-AMPLIFIED SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798">
        <property type="entry name" value="MCT-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17832">
        <property type="entry name" value="Pre-PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005067">
        <property type="entry name" value="Tma_RNA-bind_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="7">
        <location>
            <begin position="91"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00060251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00070056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR005067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="181" mass="20121" checksum="7304158495C31FE1" modified="2017-01-18" version="1">MFKKFTKEDVHTRTNIKSSVQRGLKTKFVSQFDDLEPVIDDIIPKKSQVELLKCEDKIQLYISDNEVVFFQQFDELIPSLKIIHKYASAFPSVKVDRGAIKFVLSGANIMCPGLTSKGAGLPDAPGLEENTIVAIYAEGKEHALAIGKLIMGTEEIKSVNKGIGIELLHYLGDGLWNFHAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P639</accession>
    <name>A0A1E3P639_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_61468</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040116.1">
        <property type="nucleotide sequence ID" value="XM_019183184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P639"/>
    <dbReference type="STRING" id="683960.A0A1E3P639"/>
    <dbReference type="GeneID" id="30200430"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="206"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="251" mass="27977" checksum="5C1E4934F9396599" modified="2017-01-18" version="1">MSNESQSRSDAQVESQEQGQQDDHQPHASNQQFSKSDTPTLPGPINSSMPSQYGSHLPVIVSSFPSGTALQQFQYQQQAAYVQAVQNQYYQHHQQQQPIHHLPPGPLPNGQIPQLHSVPQELNHLPSHQFQTPQRTMGAPSLPLSASALPKHGMTPIATPDSQRKRQKRNDFMGDDNAVELKNLAYRSLDLPLEEVAAQVRAVEISNTSSSSTPVPTDTKTQYKKDAARERQRQIFGMVWLLKSAKLVFLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P668</accession>
    <name>A0A1E3P668_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Presequence translocated-associated motor subunit PAM17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_13728</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the PAM complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the PAM17 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040070.1">
        <property type="nucleotide sequence ID" value="XM_019180582.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P668"/>
    <dbReference type="STRING" id="683960.A0A1E3P668"/>
    <dbReference type="GeneID" id="30197828"/>
    <dbReference type="OrthoDB" id="5970083at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013875">
        <property type="entry name" value="Pam17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28021">
        <property type="entry name" value="PRESEQUENCE TRANSLOCATED-ASSOCIATED MOTOR SUBUNIT PAM17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28021:SF1">
        <property type="entry name" value="PRESEQUENCE TRANSLOCATED-ASSOCIATED MOTOR SUBUNIT PAM17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08566">
        <property type="entry name" value="Pam17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ60863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="149" mass="17198" checksum="B320BCAB837D27DE" modified="2017-01-18" version="1" fragment="single">LSWTDFFKLRKEQRNINIGSSVITALIGSTASWGYISNIEIDPTQLIFGFDPFMIFFAGFLATTGVGYLFGPLLGSIIFKTKNSKKLPLFNAKNKIFLSKIYKHRVDPSFQSFSNPVPDYYGEKINSIKQYKQWLRDNNAYKRKTKEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P672</accession>
    <name>A0A1E3P672_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetylornithine aminotransferase, mitochondrial</fullName>
            <ecNumber evidence="5">2.6.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_77600</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.11" evidence="5"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040142.1">
        <property type="nucleotide sequence ID" value="XM_019184579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P672"/>
    <dbReference type="STRING" id="683960.A0A1E3P672"/>
    <dbReference type="GeneID" id="30201825"/>
    <dbReference type="OrthoDB" id="5419315at2759"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00109"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003992">
        <property type="term" value="F:N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000004">
        <property type="entry name" value="Acetylornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01107">
        <property type="entry name" value="ArgD_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004636">
        <property type="entry name" value="AcOrn/SuccOrn_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00707">
        <property type="entry name" value="argD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002325">
        <property type="entry name" value="PRK01278.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF79">
        <property type="entry name" value="ACETYLORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ60935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="437" mass="47962" checksum="C66484D05C95206E" modified="2017-01-18" version="1">MLRATRLRSFQRSFSTSRYLQASLKNPDPASDSKSGKFIQENSKYSVTTYSRPNVVFEKGEKAFLWDVEGRKYVDFTAGIAVTALGHSHPEISKILYDQSTKLIHSSNLYHNLWTNRLSRELVETTRESGGMHDASRVFLANSGTEANEAALKFARKYGKTIDESKTEFITFTNSFHGRSMGALSVTPNPKYQSPFAPLIPGVHVAEPTLESISKLINDKTAGVIIEPIQGEGGVRPVPTEFLIELKKLTKKHNALLIFDEIQCGLGRSGRLWAHGKLPKEAHPDIVTMAKALGNGFPIGATMITENVEKALKVGDHGTTYGGNPLGARVGSYVLSQVSDKGFLQNVSTKSELFRKELNSLKEEVDEIVEVRGEGLLLGVQFKSDPSKIVDAARERGLLVITAGGNTVRFVPALNIEEDVIKEGLAIFKDAVKAVFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6I8</accession>
    <name>A0A1E3P6I8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">VHS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_84319</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May play a role in the regulation of membrane traffic through the trans-Golgi network.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Binds to ARF1 and ARF2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039674.1">
        <property type="nucleotide sequence ID" value="XM_019185688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6I8"/>
    <dbReference type="STRING" id="683960.A0A1E3P6I8"/>
    <dbReference type="GeneID" id="30202934"/>
    <dbReference type="OrthoDB" id="2018246at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14235">
        <property type="entry name" value="GAT_GGA_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16998">
        <property type="entry name" value="VHS_GGA_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.160:FF:000003">
        <property type="entry name" value="VHS domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000008">
        <property type="entry name" value="VHS domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000024">
        <property type="entry name" value="VHS domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052653">
        <property type="entry name" value="ARF-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008153">
        <property type="entry name" value="GAE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47180">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47180:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50180">
        <property type="entry name" value="GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VHS" evidence="10">
        <location>
            <begin position="22"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="GAT" evidence="12">
        <location>
            <begin position="185"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="GAE" evidence="11">
        <location>
            <begin position="460"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="342"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="226"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="366"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="391"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="458"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ60467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="576" mass="63539" checksum="FCA531FEE0F99154" modified="2017-01-18" version="1">MSYQERQHQSSSSSLVRKIYRACRSTLAEPDLALNLAVADYVNDKQGGAPREAAVTIVNLINSRDSHTSIFALNLLDVLVKNCGYPFHLQISRKEFLNELVKRFPERPPVRYSRPQRLILGQIEEWNQTLCKTSRYKEDLGFIRDMHRLLSYKGYVFPEVKAEDASVLNPSDNLKSVAEIQKEEAVAQAAKLQELVRRGRPSDLKEANKLMKIMAGFKDDNLVDSRAQVAEDLDRLKRKADIFNEMLSASEKTGHIDTSDETLVELYSSLKVAQPKIQKIIEEEQDDEEAVQDLLKINDTVNSLVEKYNLLKAGDVQNASKLHVASAGSNVKESLNLIDFDDEETSPAPESSKQSGGVDDLLGELGSLSFGNNTTSSSTNNFGSGGDITLDFSSNQPSRATSPNYDIFNQLSSNTSSTSAAPTANPTIASSNQNAFASLDTSLIGSFASPQQNQNQQHSSTSPRSTVNESQHLKFELELKKESSNTVLAKAIFSNIGTFAISNLQFLIAVPKSLSLRLEPQSGSFLGSYAKDAVTQVFRIDSNNPSAPLKIKWKLNYVVNGSDVEETSTYTFPSTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P751</accession>
    <name>A0A1E3P751_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">THIF-type NAD/FAD binding fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_29178</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the ATP-dependent dehydration of threonylcarbamoyladenosine at position 37 (t(6)A37) to form cyclic t(6)A37 (ct(6)A37) in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HesA/MoeB/ThiF family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040468.1">
        <property type="nucleotide sequence ID" value="XM_019181583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P751"/>
    <dbReference type="STRING" id="683960.A0A1E3P751"/>
    <dbReference type="GeneID" id="30198829"/>
    <dbReference type="OrthoDB" id="10265862at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061503">
        <property type="term" value="F:tRNA threonylcarbamoyladenosine dehydratase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061504">
        <property type="term" value="P:cyclic threonylcarbamoyladenosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00755">
        <property type="entry name" value="YgdL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000125">
        <property type="entry name" value="tRNA threonylcarbamoyladenosine dehydratase 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43267">
        <property type="entry name" value="TRNA THREONYLCARBAMOYLADENOSINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43267:SF2">
        <property type="entry name" value="TRNA THREONYLCARBAMOYLADENOSINE DEHYDRATASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="13">
        <location>
            <begin position="62"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ61261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="437" mass="48765" checksum="96DAEA10895F329D" modified="2017-01-18" version="1">MTSCCGKFIAAGVTGAVVAAALSEAYHRYFESPVPKSSEKALKSIKKGPIQHDEDLIREQLARNYAFLGEDGMQKIRDEFVVVVGAGGVGSWVCTMLIRSGISKIRIIDFDQVSLSSLNRHAVATLEDVGTSKVDCLKNRLLKIAPWAHIEAINQLWHLPNAEELLLHGGPTFVVDCIDNIDTKVDLLTFAKQHDLKIISSMGAACKSDPTRINIGDISTTEEDQLARAVRRRLKKRGITSNIPVVFSAEKPDPNKAQLLPLPEEEFQKGKVDELSALKDFRVRILPVLGTMPAMFGLTIVSYILTSVAGYPIEPIEGKNRIKVYDGIFQSLAGQQTRIGADQRVPISLREVPYIVEEVFRGKSPVSGFSTRLTLSKWDPSRPVSLTNVVVLTKEEQKFHEDRVLNGGEKLEDVYDEKVLNLVEKRFREEEYYENFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P760</accession>
    <name>A0A1E3P760_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome c oxidase copper chaperone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_27812</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COX17 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039999.1">
        <property type="nucleotide sequence ID" value="XM_019181413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P760"/>
    <dbReference type="STRING" id="683960.A0A1E3P760"/>
    <dbReference type="GeneID" id="30198659"/>
    <dbReference type="OrthoDB" id="1915887at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016531">
        <property type="term" value="F:copper chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1130">
        <property type="entry name" value="CytochromE C oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007745">
        <property type="entry name" value="Cyt_c_oxidase_Cu-chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05051">
        <property type="entry name" value="COX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 8" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR607745-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="56" mass="6250" checksum="7B865740066F67BD" modified="2017-01-18" version="1">MSQPTTTESKPKPCCVCKPEKETRDQCLLINGQDSGKCDDIITQYKSCMKGYGFTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7P9</accession>
    <name>A0A1E3P7P9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 19">3-isopropylmalate dehydrogenase</fullName>
            <ecNumber evidence="7 19">1.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">WICANDRAFT_61466</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17 19">Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:32271"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.85"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="19">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="19">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 19">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 19">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.85" evidence="7 19"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040114.1">
        <property type="nucleotide sequence ID" value="XM_019183182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7P9"/>
    <dbReference type="STRING" id="683960.A0A1E3P7P9"/>
    <dbReference type="GeneID" id="30200428"/>
    <dbReference type="OrthoDB" id="419183at2759"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00072"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000006">
        <property type="entry name" value="3-isopropylmalate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004429">
        <property type="entry name" value="Isopropylmalate_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00169">
        <property type="entry name" value="leuB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42979">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42979:SF1">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="16 19" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9 19" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="11 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 19" id="KW-0520">NAD</keyword>
    <keyword evidence="13 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="20">
        <location>
            <begin position="5"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00019276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU004445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ60907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="364" mass="38955" checksum="A610AEBB0E66135B" modified="2017-01-18" version="1">MSEKTIVVLPGDHVGTEITNEAIKVLKAIEEVRPNVKFNFQHHLIGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGAVRPEQGLLKIRKELNLYANLRPCNFASESLLELSPIKAEFAKGTDFTVVRELVGGIYFGERKEDDGSGVASDTETYSVPEVQRITRMAAFMALQHNPPLPIWSLDKANVLASSRLWRKTVTETIEKEFPQLTVQHQLIDSAAMILIKSPTTLNGIVITSNMFGDIISDEASVIPGSLGLLPSASLASLPDTNKAFGLYEPCHGSAPDLAANKVNPIATILSAAMMLKLSLDLFEEGVALEKAVKEVLDAGVRTGDLRGSNSTKEVGDAVVEAVKRILKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7Z0</accession>
    <name>A0A1E3P7Z0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH CENPB-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_61608</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040249.1">
        <property type="nucleotide sequence ID" value="XM_019183213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7Z0"/>
    <dbReference type="STRING" id="683960.A0A1E3P7Z0"/>
    <dbReference type="GeneID" id="30200459"/>
    <dbReference type="OrthoDB" id="2507562at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006600">
        <property type="entry name" value="HTH_CenpB_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03221">
        <property type="entry name" value="HTH_Tnp_Tc5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51253">
        <property type="entry name" value="HTH_CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH CENPB-type" evidence="4">
        <location>
            <begin position="93"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="166"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="173"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="189"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="377" mass="43483" checksum="EF00EF249B98C237" modified="2017-01-18" version="1">MSMLMSTPQCLNNTSGIATNSYKVSKPKTKPRTTLEEKIMILNHYHQSNKPQADTVELFKNRYSVSTSSFSEWLKDEESLRNLYNNSNEFLKKSKRKLRFKYDKINEEMDKLVEERIQNKKPINEPFLRENWAIFANRYGVEDPKRLLSFSHGWLSQFKKRHGLDKQSRMRKLSTTSLSSPNCDAASITSDTSSSTSSTDTLVEYSNNTNITNELENMSGINPMKPSISTQSFLNLDVNEDYLQTNINNYENLINNASNNFMPIQISNTPTLLQSASLSRVNSRPLQSNSIMNSSMTNLLNFTNTSTTSSSTNNIEYDLINEGDFEIFLNKYGEDFLGFNQDKYPESFKILGDLKDVFKKEKEGYNDKKLRELLFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P807</accession>
    <name>A0A1E3P807_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_62054</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040705.1">
        <property type="nucleotide sequence ID" value="XM_019183303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P807"/>
    <dbReference type="STRING" id="683960.A0A1E3P807"/>
    <dbReference type="GeneID" id="30200549"/>
    <dbReference type="OrthoDB" id="3886144at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF15">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="34"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="85"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="166"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="221"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="245"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="844" mass="94700" checksum="D2EC28ABB23419F6" modified="2017-01-18" version="1">MSTATATTKAQVPMSEKSATSSSVKPKNQKSRNGCLTCKKKRLKCDETKPSCNNCLKKNIECGGYSTNFRWKTFEETSTSFKVMKSTPKQASKKKTSLDQKKSNIPNPDQKKLEAKQVENVDQRIMMKRSLSLPESDIFQNALQQATLSIAGKTFQELAKQNELMALGKNPNGNNNSSTSVTSSSLPPETPTIDNNKIQHPQTIDPKPTNDVESAAPKQHSLPNSPINEPTTGLSPSIVDQFHYNLPSPMNHNNNKNQNKRRRLSNSVQTPNNDTFNNNNLITGLSPPAFLGSPSFSTLVRAFTDFDSMNIPSPSNIELSPKIEEAIEDDDEEDNSKSLEESQGALHSMRRANSVDSIFSSGSLPQYRKELITFSNSFNNNQQQQQDQFRLPPSFTISKKNELTTEFDKIANGFHKYTSTIMSIRDGPTENPWRTVIWPMALKHSVLFKSLASMTLLHIARNDEETKRIGMTYMKQAINELAQGLTDNSIPNEVALATCIALTVTETWSTQVTTGIAHLRGAKSIINKILKNHENSHQPISPTFKVLCNIFIYHDVLARIVSSQLIDSNAYDQNSEMIKYLKKVEAVEAEERQKSNSPESDTDEHVNRLLNGFSGVEVDDTIDPLLGCAQDLFLIIGRTASLITKTRSLNKISLSIVSNAVALKTELENWKHKQLIKQPKFEDPYCDLPSIIATAESYRYATLLYLHQAIPEIPSQTSRDLAEKVLMLLASIPTNSKTCLLHIFPLLVASCEMDDKEDRDWIIARWQVLSQLMGIGNIDRAYEIVKEVWRRKDSLNKGGDGFKWDIQDQIDALTADKRDDARGVNSWTHWSTVMKEWGWEILLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P829</accession>
    <name>A0A1E3P829_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_59643</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ61559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019182847.1"/>
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    <dbReference type="STRING" id="683960.A0A1E3P829"/>
    <dbReference type="GeneID" id="30200093"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18597">
        <property type="entry name" value="ABC_6TM_YOR1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18606">
        <property type="entry name" value="ABC_6TM_YOR1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000838">
        <property type="entry name" value="ABC multidrug transporter (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000010">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000973">
        <property type="entry name" value="Multidrug resistance-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="224"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="254"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="368"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="686"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="727"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="804"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="829"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="909"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="941"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="112"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="420"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="694"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="1000"/>
            <end position="1226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ61559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1233" mass="138806" checksum="72A6D4A31E083390" modified="2017-01-18" version="1">MKEHLEEKPSKYTPELQQRLFTPVLNKKVPPIPSKEEKTPFPKPNLLSAVLFWWLTPLYKIGYKRTLVSNDLWYLRDDIGVEKTYAIFNSHLDSGKSIMISLFLTFKYEYSFALFAKVLADLIQVLLPLLTKALVNFVEQRILNGGKVNKGIGYAIGCAVLVCIDGILVNHSLYHSMLVGSKVKAILTKSLLEKSTKTSEESRKEFTTSKVTSLMGTDLTRLEFAIGFLPMGFAIPLPVIIAIALVIINIGVSSLAGIGVFFVAIFILAFLGKQMFKLRGVANVFTDERVGLVRELIQSLKFIKFYAWEDAYEEKYQNIRTKESSVVFSMQRYRNIMMAFVLTLPSVISMVSFLVLHSVDNSRSVADIFSSLTLFGVLSQEIMMLPIIIGVVTDGKVAFKRIGEYLAVNEENIEITPDVLDDANSIEAEDLSLHNVQNATFDIKSGEFVVVVGIVGSGKSSLLKALNGKFTSGKLRYSGESIFCDEPWIQSTTIRENIIFGENFDQVRYKKVLKACSLESDLKNLPFSDLTQVGDRGVTLSGGQKARINLARALYKDVDIYLLDDVLSAVDAKVGDAIVNDCLFGILKEKTRVLATHHLSLVHQADRIIFLGETLQIGALKDFANNKEFQTLMSFGNSKKLEEKKEVFEVQERQTDKELINDEEQAVNSIKYVVYKQYLKAGNSRYYLAVPLNLLLLTLSVFTLLFANVWLSYWIEQKFHQPSGFYIGFYLMFTILCVLFTGCQFVMVAYLSITAAKRLNIGAFTRVLHAPMSLIDSTPVGRILNRFTKDTETLDNQIGEQLRLFLYISALLIGYMVLCVVYMPWFAIAIPFVVVICIGLVNYYQASAREIKRIEAVKRSFVYNNFNETLGGLNTIKSFGKVKDFLRKNDVFLNEMNEAGFLVIANQRFISIMLTLVACFLCLIILLLIVNQSFHISAEGAGLVTNYILQLVLLLSLLLRSFTDLENEMNSVERVCHYANEIEQEARYKTEDFQFQNGDLKFQNVCFRYRENLPLVLQNINLDIHSGEKIGIVGRTGGGKSTIGNALFRIGEYSGLIEINGVDISKIGLFNLRSQLTIIPQDPVLFKGDIKSNIDPFNQFGEDEINQALGKTGLKLSSNEKVEEGGLNFSLGERQLLALARALVRNSKVLYLDEATSSIDFETDSKVQKIINEEFTDCTVLCVAHRLHTILKYDRIIVLDGGEIVEIGEPKFLYQQNGLFKEMCEKSSIDLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P878</accession>
    <name>A0A1E3P878_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">WICANDRAFT_81856</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_019185301.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="30202547"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="250" mass="29066" checksum="8A7BC52C49870FEC" modified="2017-01-18" version="1">MRKPSINERVGYYSSWTAIEYYMFRRLSKILHPLNNNNSSAKVQFSFHKGENNQFFPYLCSDIYHDDQDITDSTTIGDHNDGFKVHDQQQMKICQVNIHSKSSSGVLIILRPLDSDLLKELESLLDSIQNDQSINPIRLKISKVSIPKDVELETILKYLLKNPPKEFNVTSTTLGNDELSLFTIVKNVDDDIKDVLKYHNQLKLKPLRSILNSYDVEHIVDNSRFIEFQEQLPCYSEFENGLKLPKYEMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8H9</accession>
    <name>A0A1E3P8H9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Coenzyme Q-binding protein COQ10 START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_61739</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for the function of coenzyme Q in the respiratory chain. May serve as a chaperone or may be involved in the transport of Q6 from its site of synthesis to the catalytic sites of the respiratory complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with coenzyme Q.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the COQ10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040384.1">
        <property type="nucleotide sequence ID" value="XM_019183238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8H9"/>
    <dbReference type="STRING" id="683960.A0A1E3P8H9"/>
    <dbReference type="GeneID" id="30200484"/>
    <dbReference type="OrthoDB" id="292693at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140104">
        <property type="term" value="F:molecular carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07813">
        <property type="entry name" value="COQ10p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044996">
        <property type="entry name" value="COQ10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005031">
        <property type="entry name" value="COQ10_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901:SF10">
        <property type="entry name" value="COENZYME Q-BINDING PROTEIN COQ10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901">
        <property type="entry name" value="SPERM PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03364">
        <property type="entry name" value="Polyketide_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coenzyme Q-binding protein COQ10 START" evidence="4">
        <location>
            <begin position="48"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="192" mass="22111" checksum="756EC8DB3BD83FBE" modified="2017-01-18" version="1">MLVIRRSQVKAAYGIRAFHSSKPGKFSLLKSLEKSDGEQSFVVKRVLNHPPELFYDVVSDVSQYSKFVPYCTRSFINARDQALQPTEAGLRVGWKSFDEEFVCNLQCDPKKVVIAESVTHSLFEHLYTKWTITPNARKNSCDMELLLKFNFKSALYNRVSSLFAESVSELVIKAFDKRAAQLRREMMKQERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8I1</accession>
    <name>A0A1E3P8I1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pescadillo homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Nucleolar protein 7 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">NOP7</name>
        <name evidence="8" type="ORF">WICANDRAFT_48648</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the pescadillo family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040885.1">
        <property type="nucleotide sequence ID" value="XM_019182613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8I1"/>
    <dbReference type="STRING" id="683960.A0A1E3P8I1"/>
    <dbReference type="GeneID" id="30199859"/>
    <dbReference type="OrthoDB" id="10264910at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17709">
        <property type="entry name" value="BRCT_pescadillo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000067">
        <property type="entry name" value="Pescadillo homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03028">
        <property type="entry name" value="Pescadillo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010613">
        <property type="entry name" value="PES"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12221">
        <property type="entry name" value="PESCADILLO - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12221:SF6">
        <property type="entry name" value="PESCADILLO HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06732">
        <property type="entry name" value="Pescadillo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="BRCT" evidence="7">
        <location>
            <begin position="347"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="451"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="467"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="521"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="541"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="558"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="568"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="589"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="601" mass="69524" checksum="4F8DC9BA9F2BDBC7" modified="2017-01-18" version="1">MRVKKKNTRGNAKNFITRTQAVKKLQVSLADFRRLCIFKGIFPREPRNKKKANKGSTAPVTFYYYKDIQYLLHEPVLQKFRDHKAFSKKLNRALGRGEVGDAKKLEENRPKYKLDNVIKERYPTFPDALRDIDDALNMLFLFSIMPATDKISTRVTKDATTICNQWLAYVSRERALRKVFVSIKGVYYEAVVKGVEIRWLVPFKFPQNIPSDIDFRIMLTFLEFYSTLLHFVLYKLYTDAGLIYPPKLDDKKIKGVGGLSAYILESKSTGSILPEIDEESGKDVEGQQISNESIKAALKADSKADPEQEEAQEESNQDVELIELDEFLDTNKNSGDLLKQPSKFENETSNLFNNFVFFISREVPLDILEFIILSCGGSIISDLSLDELKMNGEDISKLPLDKITHQIVDRPKINGKVQGRTYVQPQWVFDSINKSNLLPVNTYLPGETLPPHLSPWGDAGGYNPEEKLEEVEDEEESEEEDEEIEEGDEDVEDIEEDEEEEDEDLKAQKELELEAQGVKYSDVKQDLQDSDKKKRKQSSTKTKEDEEKELKLIMMSNKQRKLYKKMKYSNEQKESRQEELRQKRRKIEKAKSKLDKLNEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P968</accession>
    <name>A0A1E3P968_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_24758</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040971.1">
        <property type="nucleotide sequence ID" value="XM_019181053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P968"/>
    <dbReference type="GeneID" id="30198299"/>
    <dbReference type="OrthoDB" id="434783at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051752">
        <property type="entry name" value="Mito_2-oxodicarb_carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356:SF1">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00927">
        <property type="entry name" value="ADPTRNSLCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="2"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="101"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ61764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="244" mass="26726" checksum="081DB027DD6B4410" modified="2017-01-18" version="1" fragment="single">ALPFMYQFLAGAVAGVSEILVMYPLDVVKTRIQLQVGNGAQAEYSGVVDCFAKIIKNEGPSRLYKGITAPILMEAPKRATKFAANDEFSKIYKKAFGVQQLNQPLSILSGASAGAVESFVVVPFELIKIRVQDRSSKYKSVADAFFSTVKNEGVLALYNGLEATMWRHVVWNAGYFGIIFQIRSLLPKAETSTQKTMNDLTAGALGGTFGTIFNTPFDVVKSRIQNTVSQRILSGQLIDLELRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9F5</accession>
    <name>A0A1E3P9F5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Iron-sulfur assembly protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_87143</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the assembly of mitochondrial and cytoplasmic iron-sulfur proteins. Probably involved in the binding of an intermediate of Fe/S cluster assembly.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesB/IscA family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041228.1">
        <property type="nucleotide sequence ID" value="XM_019186094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9F5"/>
    <dbReference type="STRING" id="683960.A0A1E3P9F5"/>
    <dbReference type="GeneID" id="30203340"/>
    <dbReference type="OrthoDB" id="333486at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044572">
        <property type="term" value="P:[4Fe-4S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.300.12:FF:000001">
        <property type="entry name" value="Iron-binding protein IscA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="ATAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="ATAP_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050322">
        <property type="entry name" value="Fe-S_cluster_asmbl/transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017870">
        <property type="entry name" value="FeS_cluster_insertion_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00049">
        <property type="entry name" value="iron-sulfur cluster assembly accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10072:SF41">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 1 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10072">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01152">
        <property type="entry name" value="HESB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core" evidence="5">
        <location>
            <begin position="138"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="49"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="70"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="96"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00054873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00071673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="241" mass="27427" checksum="D26895BEC702ACE6" modified="2017-01-18" version="1">MLRLIPKPQQSYSCFVRRISDSSKSTRRFMQTAPLANNEVSYIPYKFQNRQQSQQASSEQHSQSQPPSGWERHSLTSRSFKDILKKNPQKQPTSPVEKEQQQEKQVKEHGKQAEAATTPAAKPKKRVRRTLKPRKALITLSPTAIQHLKGLLDQPEPKLIRIGVRNRGCSGLTYHLEYITEPNKFDEIVEQDGVKVVIDSKALFSIVGSEMDWLDDKISSRFIFKNPNSKGTCGCGESFMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9L2</accession>
    <name>A0A1E3P9L2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_82139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041197.1">
        <property type="nucleotide sequence ID" value="XM_019185339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9L2"/>
    <dbReference type="STRING" id="683960.A0A1E3P9L2"/>
    <dbReference type="GeneID" id="30202585"/>
    <dbReference type="OrthoDB" id="3979935at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013933">
        <property type="entry name" value="CRC_Rsc7/Swp82"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08624">
        <property type="entry name" value="CRC_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="534" mass="60269" checksum="C00D3DA9E62D956F" modified="2017-01-18" version="1">MADNDKPSEPASEIEASLPPNTPENPQPTLFRVEKSDLYEHTQHIRTFPKNSDVKAPVVGEFQNLDKLNDTRDEYECTKTDPKGEKKIDENGFLLGGRKYIITTFTLPGRGKKLYVLGTEAAKFLNHRDAFVLFNRFKQLYKIKANDEERKFLEDCNFTISKIKSRSVAVTTAKNLFMLFGARIVKSGKRVEDDYWEDAAIEQGFKPTDQVFPLEKGKSEVVKPKVAAPKPVPKVPKVNLVTPMPPLEDRRDYLLASSSGNPAQAIPGQGITGGIELATVATIPKYKQDSTNTVKHRSILSTLSSNNSSTSLASQAAANQIPINDAFAGEKSTGGLPYYNSNVLQRIMDEDSEKIREIEYLHGTVETNNYISLSRKPRVKQWQYYWQVKSGAPIGLLESQKDEYFKEQEESLNKVQEETIYNEFLYCDQVIKKRRKPNPNYLNHGNINGLKPPYIEKEPTPALEQAMPQHIAQPYGSVPQVAAQMGSTTQQEYYQRARPNDPYAQYTATERGIPQAATAQQIYEAQRSGIQFQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9U2</accession>
    <name>A0A1E3P9U2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mediator of RNA polymerase II transcription subunit 20</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Mediator complex subunit 20</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MED20</name>
        <name evidence="5" type="ORF">WICANDRAFT_76251</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the Mediator complex subunit 20 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9U2"/>
    <dbReference type="STRING" id="683960.A0A1E3P9U2"/>
    <dbReference type="OrthoDB" id="1854899at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.180">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013921">
        <property type="entry name" value="Mediator_Med20"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08612">
        <property type="entry name" value="Med20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="205" mass="22724" checksum="52ADEDDB88F90CE7" modified="2017-01-18" version="1">MVTTAVLLVENASPNSITAFHDAISNEIPNILGRWSFELKIFKSNTQHLQSSQSNFLYNLSLGHQPNKVVTLVNKSAIVTTSDIPKPLIDNGCSNGSVDNLDHIIQTKLQSLWLLRQTLKGENGNSYELMNSNLIIRTINVFLHGTFKAFLIQLEYSGTGNGREKIEEFTSLLNIPGGKLCTGILNEKPDYLGDLALQYTDALQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA03</accession>
    <name>A0A1E3PA03_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_82167</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041251.1">
        <property type="nucleotide sequence ID" value="XM_019185342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA03"/>
    <dbReference type="STRING" id="683960.A0A1E3PA03"/>
    <dbReference type="GeneID" id="30202588"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17327">
        <property type="entry name" value="MFS_FEN2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000064">
        <property type="entry name" value="MFS allantoate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF40">
        <property type="entry name" value="THIAMINE PATHWAY TRANSPORTER THI73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="336"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="360"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="46"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="484" mass="53901" checksum="B33660E546D388B3" modified="2017-01-18" version="1">MLHKKGDNKLDKDEAIKFLSDTKVTILSDGPSKYPKKLIRKIDWRIMTVMCGSYFLQFLDKNLLNYAGVMGIKKNLKGNQFADLGTIFYAAYIFAEPLSDYCLQVLPTGKFLSCCIIAWGVVVACHAACHTYSSLMVVRTLLGFFESSLSPGLIVISSMWYNKQENLQRTGLWVSTAGLSVIVGGLLSFGFQHIHSDFESWRIFFLVMGVITIAFGIFTYFFLPNNPTSASFLTEEEKILVLEHIRGNQTGTETKKFKIKQIKELLFKDKHTWPLFFLTTISMISTGALGTWSVTIIASFGFSSEIAALVQMPVGAAMVIAILTESYLCSYIGRRTLIFTGMCVPGIVGYIILLTTKNKVANLIAIYLNMGSTGVIALLYSWNSANTAGHTKKLARNGLTMIAFSLGSLIGPQLFRAHEKPDYRSAKVTLLITSIVCVPLSLIVGLISKRENEKKDKEGPVPLPPNYEFKDLTDIENPNFRYSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA84</accession>
    <name>A0A1E3PA84_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_26626</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041532.1">
        <property type="nucleotide sequence ID" value="XM_019181282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA84"/>
    <dbReference type="STRING" id="683960.A0A1E3PA84"/>
    <dbReference type="GeneID" id="30198528"/>
    <dbReference type="OrthoDB" id="271164at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06861">
        <property type="entry name" value="PX_Vps5p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000013">
        <property type="entry name" value="Putative Sorting nexin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000022">
        <property type="entry name" value="Sorting nexin 3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037868">
        <property type="entry name" value="PX_Vps5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015404">
        <property type="entry name" value="Vps5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09325">
        <property type="entry name" value="Vps5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="12">
        <location>
            <begin position="16"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ62325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="402" mass="45955" checksum="D4D6CA7807282E43" modified="2017-01-18" version="1" fragment="single">QSKIHQKQKQEKEPAVKLDISVGDPIKVGDLTSAHIVYSVRTNTDSELLRSPQTVVSRRYRDFRWLYRQLQSTHPGRIIPPPPDKQAVGRFNDDFIEARRFALERMLVKISKSPVLQTDPDFIMFLQSERFSSEAREREKAAGATSTFTASEKDNDASLNASGGFLSSIGGAFSFSPKIVEPDEFFKDKKSYVEALDQQFKLFLKNLETIVIQRHDLSIVTEEFANIVATLADLEVSKSSTELFQEFSTTQLRIKELLDRLSLQDMLTLGSTLDEYQRVIGSIKTTFHQRDKILVQLAGSESELKKKQASFDKTFKYNRTHTEKIDALKLELDALEQKHNGIRSKFDDISSTIKDELATFEIEKIQDFRNSIEIFLESTIESQKEAVELWETFYDVNLSSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAJ9</accession>
    <name>A0A1E3PAJ9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_60493</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041642.1">
        <property type="nucleotide sequence ID" value="XM_019183003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAJ9"/>
    <dbReference type="STRING" id="683960.A0A1E3PAJ9"/>
    <dbReference type="GeneID" id="30200249"/>
    <dbReference type="OrthoDB" id="3993941at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="591"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="271"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="596"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="611" mass="69024" checksum="86F38AED6FF2BAC1" modified="2017-01-18" version="1">MNSSGTVKSSFDNRRRKHSADSGILPSNGVDAIPKSSIVEESHLSNSQQASLDSRDLLSNPNSLTKNNKSYQSGTEKSSHSSYRSNSHEPKTPIDSEQQEDNNFNLSDFAADDMESIKNPSELLLYEPAHEMSELYDSVDNWSPMESIDIPEYSQYDMDNYRSLEPSLYETTLLQRDTQPQDLIENLPIERRISHYSQDPLSFLMSQDKVINLHLQQNIQSHAEVSKKGLSSKPIRHNIENCLKSLSSVYQSIAELYQKEQKRKSIILKSFEKWEQNKKILNEKVQDIRSENNTEGYRLTQLLKESTTVDEEIESLEKRLKQLKEKKKVLGNEIAQSQSIIESRSSSYLESLREIEKVEKNAIIKLSHERSIESGLSNSSLGFPIGNNTRQKFGLKNFLRSFSTPLDSTFDEVDAAPVIELIERQIDTFKDCIDDYKLKETSYEEAFIIWADISTILNGLETTIYDILQQNGSSKDDIKSKIVDNLTQTQKLLIERLDSINNMNGQLKSLIHTEISILDMGIKMIQPDHKSNAPSPPDDTKEAQVLAPTVNPTVAKFSISDKALASSPPHATLAVGSGSFSSSGTTLKSQIKNGLFKKDVTKDKEKQTKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAL3</accession>
    <name>A0A1E3PAL3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_76598</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041638.1">
        <property type="nucleotide sequence ID" value="XM_019184417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAL3"/>
    <dbReference type="STRING" id="683960.A0A1E3PAL3"/>
    <dbReference type="GeneID" id="30201663"/>
    <dbReference type="OrthoDB" id="1939598at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089713">
        <property type="term" value="C:Cbf1-Met4-Met28 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14705">
        <property type="entry name" value="bZIP_Zip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13044">
        <property type="entry name" value="ACTIVATING TRANSCRIPTION FACTOR ATF 4/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13044:SF14">
        <property type="entry name" value="CRYPTOCEPHAL, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="275"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="50"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="201"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="287"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="210"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="351"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ62431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="367" mass="40273" checksum="DD926FF150485F98" modified="2017-01-18" version="1">MSNTNNNENITPAALLEQLVYVDTFMPEIDDPSQLDERLTAELSAFADESFIFPDEEKPSKPSDSDATKSTGTNGADNSKNRSPNDEEILKRFDEMNQSFANNQIIHPQQPKFNTLQQTNSLQSGISNILSPLSTQQQSSNHNPITMNPIHTSNNSSGNDVLLNLPKVPVPPGAQSSLIAAGLSQTQIDALAALVAQHQGVDSNDPKSQDVGSLNSLINPSQSLINPSSTGSNSGTPISPNDLGLQSQQQQQYGQHSRSNSIVKSEIGGEVDVDKRRRNTAASARFRIKKKLKEQQLERNVRELNELTLTLEKKIQTLEMENKLLRNLVVEKGEQKNNDELEYLKKRAKLSVSNDNSNGQRGSASSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAS7</accession>
    <name>A0A1E3PAS7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cysteine protease</fullName>
            <ecNumber evidence="11">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_18503</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:67548"/>
            <dbReference type="Rhea" id="RHEA-COMP:17323"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67549"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase C54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="11"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041184.1">
        <property type="nucleotide sequence ID" value="XM_019180792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAS7"/>
    <dbReference type="STRING" id="683960.A0A1E3PAS7"/>
    <dbReference type="GeneID" id="30198038"/>
    <dbReference type="OrthoDB" id="2960936at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF49">
        <property type="entry name" value="CYSTEINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 11" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="12">
        <location>
            <begin position="8"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ61977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="297" mass="34041" checksum="40E3439C8F5EB4A3" modified="2017-01-18" version="1" fragment="single">KESNIWPQEFIDDVYTRIWLTYRTKFAPINRDEDGPSPISLHNLIRGQNYDLNSEYFTTDCGWGCMIRTGQTLLANALLNLHLGRQWRFSEEANEKHEQIVSWFIDCPSHPFSIHRIVDKGRILSNKKAGEWFGPSAAARSIQALCNDFDSGLTVYIGSDSGDIYENDVFKVAKDDTGVFQPVLILLGVRLGVESINKLYWNSLKSILQSSESVGISGGRPSSSHYFFGFQGDYLFYLDPHFPQQALLHEDKLENSVIHTKKLRKIHLSEVDPSMLLGFLIKDEHEWLDWKQKIQDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAV3</accession>
    <name>A0A1E3PAV3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Enhancer of mRNA-decapping protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_60579</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the EDC family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041728.1">
        <property type="nucleotide sequence ID" value="XM_019183021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAV3"/>
    <dbReference type="GeneID" id="30200267"/>
    <dbReference type="OrthoDB" id="3981259at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028322">
        <property type="entry name" value="PNRC-like_rgn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15365">
        <property type="entry name" value="PNRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="68"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="149"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00061292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="18108" checksum="5AD9E4B0EBACBA73" modified="2017-01-18" version="1">MVHDQFKNQDTKFFNQHKNEPIRIPSPNVLPNGQKPDFGNSSRSSKKQAHHNQKPQQRLPNGQMPDFGNSPKNNNNNNKKTGASKTNNRPKSNNKNNNNNQRKSPLSSPKTNSSQINNNNYELSFQKSAPVSAASSSSSKTPSFAGSTFSNSPQTSSIPKPTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB46</accession>
    <name>A0A1E3PB46_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">3-hydroxy-3-methylglutaryl coenzyme A reductase</fullName>
            <shortName evidence="9">HMG-CoA reductase</shortName>
            <ecNumber evidence="9">1.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_99032</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(R)-mevalonate + 2 NADP(+) + CoA = (3S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15989"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Endoplasmic reticulum membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.34" evidence="9"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041818.1">
        <property type="nucleotide sequence ID" value="XM_019186676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB46"/>
    <dbReference type="STRING" id="683960.A0A1E3PB46"/>
    <dbReference type="GeneID" id="30203922"/>
    <dbReference type="OrthoDB" id="310654at2759"/>
    <dbReference type="UniPathway" id="UPA00058">
        <property type="reaction ID" value="UER00103"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="HMG-CoA_reductase_classI"/>
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    </dbReference>
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        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl-coenzyme A Reductase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3270.10">
        <property type="entry name" value="HMGR, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.420">
        <property type="entry name" value="Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
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        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023076">
        <property type="entry name" value="HMG_CoA_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004554">
        <property type="entry name" value="HMG_CoA_Rdtase_eu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023282">
        <property type="entry name" value="HMG_CoA_Rdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
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        <property type="entry name" value="SSD"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572:SF24">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="NAD-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="Substrate-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00066">
        <property type="entry name" value="HMG_COA_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00318">
        <property type="entry name" value="HMG_COA_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01192">
        <property type="entry name" value="HMG_COA_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0521">NADP</keyword>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="287"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="457"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="11">
        <location>
            <begin position="148"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="493"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="517"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="978" mass="106237" checksum="1520791FAEA9F99A" modified="2017-01-18" version="1">MAAVLERPLNGLASVLAFIGFSYADSSSNLVNYYHSSSAKSPWVQVDDFSKISQDAEHFDVIPFQFKRGGVLELPEIANSYSSDDEQVKFLITKENETTDAEITQLISSDGTVWKFRDFHAKLGKYSDLIIKGYNKVSKLILGAEPFDIAIVTVAYLAMWYTFISLFLDMRKAGSKFWLAFSALVSSFFAFLFALYATTRYLELQIPVLSITEGVPFLVATIGFKHKIALTTKLLTKYSSQEYHKHGTPSCLYLAIKEQGSALLHDHLLVITAFIGCSFYASHLEGLRNFCIFSALILFFDLLTTVTFYAGILSLKLEINLIHRSTIIKQALQEDGLTQETVDEVLRSDEDASKVYIDDNTNSAVTAAKIGAIVLFFGFHIYGLGSAWLSDLGANSEYNDSFSLDETLFANIPIGSKGTLVSVHPARFFLPSKVSIQIEDYILSTIGYISAAIRDKYISKFVLFAFAISVTINVYLLNAARIHSSSVESDIEKNKSQKLAKSAKPTPTKKVEFEPKISNNSSSNESDVTKSSEIIPTSDNETVEARPLDKIVELFKDGKVKSLQNEEVVSLVTNGNLPLYALEKQLGNTTRAVEVRRKAIAKLAGAPVLETSKLPYLHYDYDRVFGACCENVIGFMPLPVGIAGPLIVDGTPYHIPMATTEGCLVASAMRGCKAINLGGGVQTVLTKDGMTRGPCVSFPSLKRAGACKIWLDSEEGQASIKKVFNSTSRFARLQHIQTALAGDLLFIRFRTTTGDAMGMNMISKGVEFALKQMVEEFGWDDMQVISVSGNYCTDKKPAAINWIEGRGKSIVAEAIVPKEAVEKVLKSDVGALVELNISKNLIGSAMAGSVGGFNAHAANILTAVYLALGQDPAQNVESSNCITLMKETQDGDLQISVSMPSIEVGTIGGGTILDPQGSMLELLGVRGPASVPGANAQQLAKIIASIVLAGELSLCSALAAGHLVQSHMQHNRAGATKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB79</accession>
    <name>A0A1E3PB79_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_88031</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041681.1">
        <property type="nucleotide sequence ID" value="XM_019186134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB79"/>
    <dbReference type="STRING" id="683960.A0A1E3PB79"/>
    <dbReference type="GeneID" id="30203380"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="332"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="538"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="14"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ62474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="692" mass="77755" checksum="850562A433EF9007" modified="2017-01-18" version="1">MANSSNFTKSNFDSKSSLLSSLSSSRSLSKQSSSSPLNPNNSFHHQRPENEASPSRSSSTLSEIGSQDDLQIKSSISPAYKLRKTTPYGQLQDVNKSSSKLHRIYSENETSNFSQVNGRRMFDDQRHRPHRTKFFERQSEYVPDSPLQFTARSFSNFYQANTAIEAPKYKGLEDEYIPDFDFSNTIQQWTDSYYAGANYDRSYDSSLNDSMPSSMISRPLTPVGKLHSQVSPVTVPRPKREGLSIKTDEEGLTNDEELIIAKLPHNFTDLPFSVRRKLLADLSPNGNVETINKIVKKKFRKRQKSSPAVQFLSSFSDRSYKADDKENIVLDHSLGGIIGYGAWGIIRECTSPEGQTRAMKIVRVKNDAAREFFKRETIIWAQLHHDNILRLINVKESSNAIYCLMDRAYGGTLFDLVSKWGVYPSKSIQAKERLSIIKRYTKDILSGLMYMHSKGIVHGDVKLENCLVTNKTSSGKILLCDFGMSLRFMNPIDSDEVTLPKNNTKIKRRPSIPRSYSGNSLKSPKSLLKIFSERSSANEDSKINTNTDNHQKAIGSVPDSPFSPKPFEYESKQLIDATLPHSHIGSLPYAAPELLESSPPPLGPSADIWAFGVLLYTMIVGKLPFQHSFEPRLRAMIAAGKYDTDSLKIAAQNNQELYDTVVGCLTIDMNKRLNLGEVMTLIEGVNIQEDAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBC8</accession>
    <name>A0A1E3PBC8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Signal recognition particle subunit SRP14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Signal recognition particle 14 kDa protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_60254</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of a fungal signal recognition particle (SRP) complex that consists of a 7SL RNA molecule (scR1) and at least six protein subunits: SRP72, SRP68, SRP54, SEC65, SRP21 and SRP14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SRP14 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041394.1">
        <property type="nucleotide sequence ID" value="XM_019182961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBC8"/>
    <dbReference type="STRING" id="683960.A0A1E3PBC8"/>
    <dbReference type="GeneID" id="30200207"/>
    <dbReference type="OrthoDB" id="19209at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.10">
        <property type="entry name" value="Signal recognition particle alu RNA binding heterodimer, srp9/1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003210">
        <property type="entry name" value="Signal_recog_particle_SRP14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009018">
        <property type="entry name" value="Signal_recog_particle_SRP9/14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12013">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 14 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02290">
        <property type="entry name" value="SRP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54762">
        <property type="entry name" value="Signal recognition particle alu RNA binding heterodimer, SRP9/14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0733">Signal recognition particle</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="133" mass="15169" checksum="7AB5AD3D218D0C8A" modified="2017-01-18" version="1">MTLLSNIEFLKAAAELFEQSHESRSSIYITQKRLSEVDQVNGLKDTQDEISQFNDSKPILPNSTIRKSTKSYPILIRITDGNNDKSKKKKYSTTVDSEHLGKFWKEYSQVIKTGATGLKKKEKSKKKKKTVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBH3</accession>
    <name>A0A1E3PBH3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_60822</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041980.1">
        <property type="nucleotide sequence ID" value="XM_019183062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBH3"/>
    <dbReference type="STRING" id="683960.A0A1E3PBH3"/>
    <dbReference type="GeneID" id="30200308"/>
    <dbReference type="OrthoDB" id="331602at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000144">
        <property type="term" value="C:cellular bud neck septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061645">
        <property type="term" value="C:endocytic patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001400">
        <property type="term" value="C:mating projection base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990252">
        <property type="term" value="C:Syp1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007117">
        <property type="term" value="P:budding cell bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045807">
        <property type="term" value="P:positive regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032185">
        <property type="term" value="P:septin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009826">
        <property type="term" value="P:unidimensional cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018808">
        <property type="entry name" value="Muniscin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF54">
        <property type="entry name" value="SUPPRESSOR OF YEAST PROFILIN DELETION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10291">
        <property type="entry name" value="muHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MHD" evidence="3">
        <location>
            <begin position="647"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="265"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="424"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="297"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="356"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="370"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="383"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="436"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="496"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="544"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="564"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="904" mass="99501" checksum="C532534BC1D2000A" modified="2017-01-18" version="1">MSGPERNKYSTSILVNQSPIDATEIVRVRLSQAKLLNDEFSKFFKNYHTIKTNYLNQLNKLIKDSQDLNKNIEHGIIENQVLSREELKYYNVDAIGHLSQVWNEVITEIKDEVASNYRLGQVVDRDVINPLSHYTTNDKRWSEVRHLHSKLSEVAQTIEFNQEKVEKYQGGRSSDKLEKYQTSLQTANQTWASEAPYVFEVFEYTDFDRLSFLRDSLLRFQTAYSDSLNKISQSNEKALETILNFNPETEIERFAKVSSETSYVPKEARKAAESPTVPKHHSGAPIGGTTVPSGNRQASVSTTATSQTHKAKAGLESPTSTTKQPKSKLKSRVGSIFGRKNKKNKHVDVAEAVPESETSSIHSPSKTTNRLSRASSLASRLRTQQSGSNLNSHNHPEVNNHEAGVITGAVGADAAAVGAGAAAMSSGNIPRPISKESGSFQQQKPALGAQSSSSQNIPLSLNQPPLKPTSRTSSSAHLNGDVAETGYPPKPADSPQAGSFDQRSLDPSYSDSNSAINMHQPQQQQIHAGEAHDPHRAPPPPPSRKTNQFGSQNLTNIREDGDTSFESTSRGQYDHQLQQPPAVARRDIQSGLFTNLNQADLENHQNKRISSFNSFGGDSIRQLNPQLTGSSLALNHPGLFQHAALTQPGLNASIAEVINAKFKDGEQVQSQLVGEIAFNYLSDGFDIPTRSNLKIHGVDKFDKIIPNNQFLKQIDVNEFEITPNSILSRTLGGLKYLIKNIKAPIVVHPVWRFEAHQASVMLTIKLAPHIAEQLQGDEFVEVNELSVSVAVSGSLTTGALSKPQGTFNKEKSRISWKFKDPIKLSKINEEKLVARFLTNSEARESEQGVVLKFAINGQDGGKLITSDIELESQNFTEDDPFAQETWSPVPSAKTLVAGSYSALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBM6</accession>
    <name>A0A1E3PBM6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_60827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041985.1">
        <property type="nucleotide sequence ID" value="XM_019183064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBM6"/>
    <dbReference type="GeneID" id="30200310"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="116" mass="13560" checksum="14A7E618C4EE7CC6" modified="2017-01-18" version="1">MLGIPIERIGQTYKLPIDLESQDGPDLSCHCERCEYKKDSEGKVSTNFYYGVLVFPLWIMNVFLYLKSWTLDDQFEFSQQVQEEHRDEITLLQPESITSLGLINGFIGVLWDYFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBN9</accession>
    <name>A0A1E3PBN9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HAD superfamily hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_76542</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041579.1">
        <property type="nucleotide sequence ID" value="XM_019184400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBN9"/>
    <dbReference type="STRING" id="683960.A0A1E3PBN9"/>
    <dbReference type="GeneID" id="30201646"/>
    <dbReference type="OrthoDB" id="426235at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033883">
        <property type="term" value="F:pyridoxal phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43885">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43885:SF1">
        <property type="entry name" value="SUPERFAMILY HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G13290)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="24468" checksum="B2115A05A2563BAF" modified="2017-01-18" version="1">MCQRLILNPTEKGDIPIKGVIFDMDGTLCLPQTWMFAAMRDAIGLADKSIDILKFIDALSPENQTIAKKKIADVEEKAMKEMEPQPGLIELLHFITENDVSKAICTRNLIKPVNHLMNSFIPDHKFDPIVTREFSPTKPHPAPIQHIAESWNTEPKNLIMVGDSIDDMKSGKDAGATTVLIRTHANDHIRDIEQTDYVIDRLDDLIKMMQNGLDLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBW7</accession>
    <name>A0A1E3PBW7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_88973</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042075.1">
        <property type="nucleotide sequence ID" value="XM_019186172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBW7"/>
    <dbReference type="GeneID" id="30203418"/>
    <dbReference type="OrthoDB" id="10572107at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="191" mass="22237" checksum="4B3932D1162C8792" modified="2017-01-18" version="1">MSVVYSDHSTMNSPLNNNSPLYDQSPFGAGRNDLLNYSYDFTTNLQITNYEKSLIFKFLINHSFNYLITNYQNKLHASSMQNMCQFVLLLFKRTNLSLQQFQKLLIIMIRYLNKNQTIVLSIKQIIIGCLIKIMGVDSKNWSQITGLSLENLTLLKNHINLDHDEMIITEDELLELNDHMKSLVYSKFDVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC32</accession>
    <name>A0A1E3PC32_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_82870</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Coated pit</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042186.1">
        <property type="nucleotide sequence ID" value="XM_019185454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC32"/>
    <dbReference type="STRING" id="683960.A0A1E3PC32"/>
    <dbReference type="GeneID" id="30202700"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09251">
        <property type="entry name" value="AP-2_Mu2_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14836">
        <property type="entry name" value="AP2_Mu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000002">
        <property type="entry name" value="AP-2 complex subunit mu, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043532">
        <property type="entry name" value="AP2_Mu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018240">
        <property type="entry name" value="Clathrin_mu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043512">
        <property type="entry name" value="Mu2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00991">
        <property type="entry name" value="CLAT_ADAPTOR_M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0168">Coated pit</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="12">
        <location>
            <begin position="177"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ62979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="455" mass="51510" checksum="6A1C68D72B8041D7" modified="2017-01-18" version="1">MINALFIFNQKGDVIISRLFKDGVKRNISDIFRIQVISNLDLRSPLLTLGSTTFLHIKHSDLWIVAVTRSNTDASVIFEFLYKFQSLLVQYFGDLTDEVIKDNFSKIYDLLDEILEFGYPQPMDFNNLKQIIPPPKDIVHSGLRPILNSNKSSSKVNSHRELSTDIPWRKSDIKYRRNEIFLDVFENINVLISKDGRIIKSYIDGHIDMKTHLSGMPVCKFGLNDALSLGEDDESNGDFLAQYDVKNKKAIPKAAAGSVILEDCKFHQCVQLDKFDTDRLINFIPPDGEFELMRYRASENINLPFKLTPMITETGLSKYEFRLNLKSLFPSKLSATDVVVKIPTPPSTIDVSFGSSGGKTKFSTQDGAIFWKFNKFSGLTEHSLTANITVASKSSSSSTTMNSNSRPPITLDFELLMFSSSGVVVRFLKVVEKSNYSTIKWVRYISQGGSYEVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCC1</accession>
    <name>A0A1E3PCC1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">25S rRNA (Uridine(2843)-N(3))-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_82908</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042235.1">
        <property type="nucleotide sequence ID" value="XM_019185461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCC1"/>
    <dbReference type="STRING" id="683960.A0A1E3PCC1"/>
    <dbReference type="GeneID" id="30202707"/>
    <dbReference type="OrthoDB" id="6419443at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070042">
        <property type="term" value="F:rRNA (uridine-N3-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021463">
        <property type="entry name" value="Methyltransf_34"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11312">
        <property type="entry name" value="Methyltransf_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ63028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="35168" checksum="31443EFEE2B4B071" modified="2017-01-18" version="1">MSKRAEISEFPTIPASSTYSLPSNDVLKLFQDSFRYLLNSPNIESFIQTVKGDLYERDYIKAFGDDDRRMAYVVRWTPSRALCYSSLFSSLEEIRNIITERDSRILCIGGGACGELVGLSAVFAQSRDFYNKERYRSKLDVKLIDIADWGLIADKLTSEIKKAWLYNEPDYLNVEFLNEDVLNANHDSSLQLNSLDLITLLFTTNELFSEDKAKSIRLFQALDNCQQGAHLLIAESAGSYSHITVGTKKFPIRFLIDTILLGKDGSGNWELVKQSDSCWYRCEKDYDYPLKVENMRFFFRLYKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PD37</accession>
    <name>A0A1E3PD37_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_60846</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042004.1">
        <property type="nucleotide sequence ID" value="XM_019183066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PD37"/>
    <dbReference type="GeneID" id="30200312"/>
    <dbReference type="OrthoDB" id="10487662at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF95">
        <property type="entry name" value="F-BOX PROTEIN YLR352W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="952" mass="107682" checksum="7B49FB8E049C00A1" modified="2017-01-18" version="1">MNSLDFGKYPKALKIMASDVDLIENITFAELTDLVIVSTGGQDPRESSELSTIRNIELPKLKNIIIRGVSYLINIKSSSDVERFHLSNSVEETTHLQTLHFPYLKFLVINGTHIIGLNSLYAPDCHRLTVNDFEIFDERSHILNQINHLTCYSSRTALERIDSNELVCLEVGQLTKEDLQLITSMDFPKLSKLMIDFGEHNMDDMGCSISFAPMLTDVELLYSTGLGFLRNTWSTLKNLKLELCGSWSLYDATLDNLETFELQVGESSDFVEISNCHFPKLITLAVCSDWFGGQTEGVFENIKAPLLKSIVYEMSVDTLDLTMSCSNLKSVDIQNVSCLVLGKNQSLKEFTSRDNYLKRHLQGFATIVSDIDYDPSLFPKYCDYINIHRFSPELVRSEVVFFVIQDLANPSLVNKISQIQTTPTSFILHNNYIHVLNCPDDDRFGWGDDADLEMTAKEAYDFYYPLSGEWESILENSTELLQLAHSQKFLKSVEFPYIKRLTLDKVDINSVKLKTPSLKELTLRNCTSTDESPQFTFPLLSNLDLEGASKCLIEKLDFGAYPDEKLSIVYGEISLMRNLTIGKAKTVSITVDSDIFMREWTSFMDISLPNCMQLYLDGFQNIKSISAPLLSLLSIQHINDREFHLNNFAAKHLESLILRNLMIAHFSDADFQKLEYLDIFECTMGSVTGCFPRAETVRVSSCFLDDCFKNLDTQNLTIFEISRCEFNTGDNTTVLPPALFPSLKILCIVQFPKDGHLNIQAPNLEELCLQDPLCPIEQLCNKFPKLQALSIAANSSTIMRNVTIHGIKKVELLSSALDPTFQLVDCSFPTLEQFKAIGPAGSFIIEPCEDILDFRAPNLKSYKGNNLRIDKLDLSKFPQLRELEIGGVIELTLGEVSQNLVEFTLNGVSLKELQYSKDPENIVNFDRPAMYPTDLEPILVKFEDYASANKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PDG7</accession>
    <name>A0A1E3PDG7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">54S ribosomal protein L27, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_77076</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL41 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042124.1">
        <property type="nucleotide sequence ID" value="XM_019184495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PDG7"/>
    <dbReference type="STRING" id="683960.A0A1E3PDG7"/>
    <dbReference type="GeneID" id="30201741"/>
    <dbReference type="OrthoDB" id="408933at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019189">
        <property type="entry name" value="Ribosomal_mL41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21338:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21338">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN L41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09809">
        <property type="entry name" value="MRP-L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="16687" checksum="C95339CF13B87704" modified="2017-01-18" version="1">MKASQILNFQQSPVASLLRPWKKFRDGTLFYGVAKSGSRRHPLTTKKGNKHFYKGTRSSGIGKFTTKGRYMINWDKVRTFVVPTEFNPNLKPLVSPNAPQIQNTFAGYPKGPLDPKLQFTKIKEYILYGKVETEESALKDKYLERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU65</accession>
    <name>A0A1E3NU65_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_37121</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454220">
        <property type="protein sequence ID" value="ODQ56668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035875.1">
        <property type="nucleotide sequence ID" value="XM_019182520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU65"/>
    <dbReference type="STRING" id="683960.A0A1E3NU65"/>
    <dbReference type="GeneID" id="30199766"/>
    <dbReference type="OrthoDB" id="417252at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016650">
        <property type="entry name" value="eIF3e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10317">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21357">
        <property type="entry name" value="EIF3E_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="4">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ56668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="97" mass="11084" checksum="803F4D5BCA162598" modified="2017-01-18" version="1" fragment="single">ENARHLISEVYCRVHQRINLDELSKSLNLTREEGEKWIASLIKETRMDAKIDESEGTVIMNHPSTSVYQQVIEKTKGLSFKANQILATALQKQDSVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUE6</accession>
    <name>A0A1E3NUE6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endoplasmic reticulum junction formation protein lunapark</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_81601</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in determining ER morphology.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The C4-type zinc finger motif is necessary both for its ER three-way tubular junction localization and formation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the lunapark family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035972.1">
        <property type="nucleotide sequence ID" value="XM_019185267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUE6"/>
    <dbReference type="STRING" id="683960.A0A1E3NUE6"/>
    <dbReference type="GeneID" id="30202513"/>
    <dbReference type="OrthoDB" id="1725934at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098826">
        <property type="term" value="C:endoplasmic reticulum tubular network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071788">
        <property type="term" value="P:endoplasmic reticulum tubular network maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903373">
        <property type="term" value="P:positive regulation of endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040115">
        <property type="entry name" value="Lnp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019273">
        <property type="entry name" value="Lunapark_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22166">
        <property type="entry name" value="ENDOPLASMIC RETICULUM JUNCTION FORMATION PROTEIN LUNAPARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22166:SF12">
        <property type="entry name" value="ENDOPLASMIC RETICULUM JUNCTION FORMATION PROTEIN LUNAPARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10058">
        <property type="entry name" value="Zn_ribbon_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Lunapark zinc ribbon" evidence="3">
        <location>
            <begin position="194"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="250"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ56765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="275" mass="31322" checksum="1A2F1A4F63970945" modified="2017-01-18" version="1">MSWLSFLKSKDFDPSSFEKELANISSKISKNQRSIGSFKYQERQSKSFMIIYGSLSYLFILLYFLVKNGLQLNLYHQKEIALIIGTPILLISATKTIIFYYAKRISRLESHIEHLKSQHESKIEELKNKTNFQSTQALLNRFTDGNDLNAQLDEELLAKQQQLEEIKKLSLEGNNEAQRFNAYSQAGSSGKRHWYDSIIDTVVGADELSPNNRFALICINCSSHNGLAPPGMKPEYVKYICPRCGVLNGKDPEPAPQVIVEDVDEETTNDSKKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUV2</accession>
    <name>A0A1E3NUV2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Meiosis protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_65755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036080.1">
        <property type="nucleotide sequence ID" value="XM_019183980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUV2"/>
    <dbReference type="STRING" id="683960.A0A1E3NUV2"/>
    <dbReference type="GeneID" id="30201226"/>
    <dbReference type="OrthoDB" id="3980894at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28527">
        <property type="entry name" value="MATING-TYPE SWITCHING PROTEIN SWI2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28527:SF1">
        <property type="entry name" value="SWI5-DEPENDENT RECOMBINATION DNA REPAIR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ56873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="40055" checksum="E00AFA1D1D129E5A" modified="2017-01-18" version="1">MSDFAESPSRTEFKTPNAKSVNLTAEATTRGDDDDEKDTSVMIPHKSSPVVQLPPVQSDDNPQLIVKKKRKLQLVADPDETVEPSQVIPSKSRSSSNVSTRIPSLNEISKPELSPPSLNLSYRSFEEKESPTTAPSSSSKDQIVANTASAATRNFSSPIRLDQNAITSTPDDPKKLMMSLRRQENSVDSELVKARKKLETLRRAKVILSKKDAKNNEELIDKWRDAAQRASNYLLNASVEKINKAGGKKEYERREREKLRDNLEFTMDTSFQERISEVTRSEEYEALPEDEQERILQDLEEEAEKSMRQLEKDLDAKNKHDDDNDDNDDEFTMKDLYKRLKLDYKLVFPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV11</accession>
    <name>A0A1E3NV11_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UDP-galactose transporter homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_85984</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036246.1">
        <property type="nucleotide sequence ID" value="XM_019185979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV11"/>
    <dbReference type="STRING" id="683960.A0A1E3NV11"/>
    <dbReference type="GeneID" id="30203225"/>
    <dbReference type="OrthoDB" id="1601at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005459">
        <property type="term" value="F:UDP-galactose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005460">
        <property type="term" value="F:UDP-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120112">
        <property type="term" value="P:UDP-glucose transmembrane transport into endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013657">
        <property type="entry name" value="SCL35B1-4/HUT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08449">
        <property type="entry name" value="UAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="338" mass="37609" checksum="7F504BD1584AB83C" modified="2017-01-18" version="1">MSESSHRKSIIVLLICIGGIYASFLTWGFLQEQISSQKFGPDAQVFHAPFFINLIQNLFGFIVGCIYLSYKNSYVNKNERVSVFQSQLLKSLTLVALTQSLSSPIGLSSVEHVDYLLYTLAKSCKLIPVMVVHIVLYKKSFPPYKYLVAGIVTAGVVLFTLGNPSKKTGNSSSYGNVPLGLFYLSISLLLDGLTNSTQDILFKDNKKLTGAHLMCGLNFVSFWLTLGYAGLLSNQLHYALNFINTYPLILKEILLYALFGAIGQIFIFITLEKFGSLILVTVTVTRKMMSMLLSVFLFGHDLGVYQWTGLFLVFGGIGFEAFLKFQQKPDATLKVKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV68</accession>
    <name>A0A1E3NV68_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Signal recognition particle subunit SRP68</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_65303</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SRP68 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036245.1">
        <property type="nucleotide sequence ID" value="XM_019183890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV68"/>
    <dbReference type="STRING" id="683960.A0A1E3NV68"/>
    <dbReference type="GeneID" id="30201136"/>
    <dbReference type="OrthoDB" id="10255118at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15481">
        <property type="entry name" value="SRP68-RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.40">
        <property type="entry name" value="Signal recognition particle, SRP68 subunit, RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026258">
        <property type="entry name" value="SRP68"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034652">
        <property type="entry name" value="SRP68-RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038253">
        <property type="entry name" value="SRP68_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12860">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 68 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12860:SF0">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16969">
        <property type="entry name" value="SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038995">
        <property type="entry name" value="SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0733">Signal recognition particle</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="507"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="512"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ57038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="536" mass="62073" checksum="D5B1535E6B7C14DF" modified="2017-01-18" version="1">MSESPLALTFGARNSAFLQTSEDFRKQRVKVTRRIHKLKRYLKIQIKDTKNYKSKKITSDDYSSDNKYYLLSILEAERDYLYALEIKALLDLNSSTNAQSKQKLLSTRLRKSLKISQNLVEISQNESDETKRLEILVYKELLQAYLAIVSKKWKVAISSYSLARIGLKFLEKHGDIAENKILYKDIIEETIDEPFKLATYRDSKSISIDLNEYSKIIANESAKNSETFKFIDAKDSSFLKADEEAELIKEIDWRSYTARVKDETTSRLLSRAQNVKFDDLESYDNALILWQEALENHSSFISKADQNDDEEQDDQILLAYIKYSSLITRLRRDQTLLSGNKKESLRILDTIITTINEIKELPGVYSDDELFQNLELLSQYYQGLKLKMIGQIYFDDKSYKESLLLCTKSLESLKDAKFTVEIEFISNKDLQTLQSDILTLKQNSHVLAQIEQGLSKDKSVADDVYKFSNDYKNIVNFQIEAAPVKPVLFDVAFNYISYNKSDTKTQFQKQTTQEKAHASQEQTENKKKGFFGIFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVG0</accession>
    <name>A0A1E3NVG0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_98197</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454217">
        <property type="protein sequence ID" value="ODQ56682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVG0"/>
    <dbReference type="OrthoDB" id="3444765at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ56682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="61" mass="7228" checksum="C18CAC4779FC5FB8" modified="2017-01-18" version="1">MKNAAKCDTYCELQIFVNHRIFERTLHPLVFQRVCLFERHFSLKPSGLVLSDTLSRVNLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVI3</accession>
    <name>A0A1E3NVI3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA wybutosine-synthesizing protein 4</fullName>
            <ecNumber evidence="5">2.1.1.290</ecNumber>
            <ecNumber evidence="4">2.3.1.231</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_36333</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>7-[(3S)-(3-amino-3-methoxycarbonyl)propyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine + CO2 = wybutosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:37119"/>
            <dbReference type="Rhea" id="RHEA-COMP:11844"/>
            <dbReference type="Rhea" id="RHEA-COMP:11847"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73544"/>
            <dbReference type="ChEBI" id="CHEBI:74275"/>
            <dbReference type="EC" id="2.3.1.231"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-(3-amino-3-methoxycarbonyl)propyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36903"/>
            <dbReference type="Rhea" id="RHEA-COMP:10379"/>
            <dbReference type="Rhea" id="RHEA-COMP:11844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73543"/>
            <dbReference type="ChEBI" id="CHEBI:74275"/>
            <dbReference type="EC" id="2.1.1.290"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyltransferase superfamily. LCMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.290" evidence="5"/>
    <dbReference type="EC" id="2.3.1.231" evidence="4"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036378.1">
        <property type="nucleotide sequence ID" value="XM_019182422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVI3"/>
    <dbReference type="STRING" id="683960.A0A1E3NVI3"/>
    <dbReference type="GeneID" id="30199668"/>
    <dbReference type="OrthoDB" id="47172at2759"/>
    <dbReference type="UniPathway" id="UPA00375"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007213">
        <property type="entry name" value="Ppm1/Ppm2/Tcmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46529">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46529:SF1">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13418">
        <property type="entry name" value="Beta-prop_TYW4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04072">
        <property type="entry name" value="LCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ57171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="681" mass="77040" checksum="5E9DD9E88DA69AC2" modified="2017-01-18" version="1">MAQNEEAKPELTEKQKRKIEKDLRRKKFSDIVIQGTNDYSIVSKRSVEKLYTSALNKSAHGQSPPEYFKHFVKKFQRRSPTINRGYWTRMESIKQSTFKIIQNALSQEKKVVIINLGAGYDPLAFQYLDPQNPENSKHLGKLSFVDVDYPDLNKIKTQMIDNSNELKEIIGERIRSSIEGVELKTKNYTVLSCDLKNIDLFLKQLRSLDLDSENVTKIFIAEVSIAYMLPKFADPVISATSKLPDSHFLCLEQLLPAGEYQGFGRTMLFHFSKLNSPLNSVSTYPTIPLQIERFKSCGYPAAEATDLMGFWNSLSKDLRKRVEEIEAFDEMKEFIHFAQHYIILHATNSNFTLLDKNIQVPFNAEKSELHLDFKLQDRIPNLERKFPAATVSKDGTILLNGGASISRLKSTMVGSKLENSWEIDDTQLSPRMAHSLDQLEDGSYLIVGGRHAPNRPLSECSVLKEHDGSFKWIESPPLPEPRSRHSTFSDQNNLFVYGGNFSASPFLKFHNNKWQAMTTLGEIDSKTSSAVAYNGIKGVIIGGMDGDYNITDSLHVFEIQGNTINIDFCIEHPLLSRYGAKVQFISDDVLLLIGGVSDMMLHNQETTIVEIILSTKEVKAVEISDEVWERLPLLVGFELLKYEDSLVCIGGGEVCYSFGSIWNDILVLGKSKVPTFKLQNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVQ8</accession>
    <name>A0A1E3NVQ8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="3 11">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_35880</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="3 11"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036458.1">
        <property type="nucleotide sequence ID" value="XM_019182366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVQ8"/>
    <dbReference type="STRING" id="683960.A0A1E3NVQ8"/>
    <dbReference type="GeneID" id="30199612"/>
    <dbReference type="OrthoDB" id="60984at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000015">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000111">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF0">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000110">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 11" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="6 11" id="KW-0521">NADP</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="12">
        <location>
            <begin position="14"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="13">
        <location>
            <begin position="194"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ57251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="499" mass="56800" checksum="7F641445839713E0" modified="2017-01-18" version="1">MPHVEFKEHTVITVFGASGDLAKKKTFPALFGLFREGYLSSTTKIYGFARSKLSTDEVKERITPFLKTPTEESKGKVSEFLSLITYISGPYDSSEGFKQLNSEIEKFEAEKKVEEPHRLFYLALPPSVFVDVGKQLKEHVYAKNGITRVIVEKPFGHDLKSSRELQEQLAPLFTEEELYRIDHYLGKELVKNLLVLRFGNEILNASWNRDHIKSIQVSFKEPFGTEGRGGYFDDIGIIRDVMQNHLLQVLTLLTMERPVSFDPESVRDEKVKVLKAFQQINLNDIIVGQYGKSEDGSKPAYTDDETVKPGSKAVTYAALPFKIQNERWEGVPIILRAGKALNDGKVEVRVQYKPVASGMFDSIPPNELVIRIQPNEAIYIKLNAKQPGLSTSTSLTDLDLTYKNRYTQFWIPEAYESLIRDALHGDHSNFVRDDELDVSWKLFTPLLEYLEGPEAPTPISYPYGSRGPETLHEYLKEHGYVFTSEGTYQWPITRPQGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW26</accession>
    <name>A0A1E3NW26_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Multifunctional fusion protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="8">Delta-1-pyrroline-5-carboxylate dehydrogenase</fullName>
                <shortName evidence="8">P5C dehydrogenase</shortName>
            </recommendedName>
            <alternativeName>
                <fullName evidence="8">L-glutamate gamma-semialdehyde dehydrogenase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="7">L-glutamate gamma-semialdehyde dehydrogenase</fullName>
                <ecNumber evidence="7">1.2.1.88</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_35432</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-glutamate 5-semialdehyde + NAD(+) + H2O = L-glutamate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:30235"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="EC" id="1.2.1.88"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.88" evidence="7"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036616.1">
        <property type="nucleotide sequence ID" value="XM_019182305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW26"/>
    <dbReference type="STRING" id="683960.A0A1E3NW26"/>
    <dbReference type="GeneID" id="30199551"/>
    <dbReference type="OrthoDB" id="5322683at2759"/>
    <dbReference type="UniPathway" id="UPA00261">
        <property type="reaction ID" value="UER00374"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003842">
        <property type="term" value="F:L-glutamate gamma-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000006">
        <property type="entry name" value="1-pyrroline-5-carboxylate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000005">
        <property type="entry name" value="1-pyrroline-5-carboxylate dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005931">
        <property type="entry name" value="P5CDH/ALDH4A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050485">
        <property type="entry name" value="Proline_metab_enzyme"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01236">
        <property type="entry name" value="D1pyr5carbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42862">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42862:SF1">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0520">NAD</keyword>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 7" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="92"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="587" mass="64633" checksum="69E4C98C2E8A8B36" modified="2017-01-18" version="1">MSVAKSNFLNATKTLSLASRLNCKGFAQSKRSIGQLATPQVPPIKNEPVKPFGKTDTVDWDLLRASILKFTDNGSLDIPLVINGKRIYGKGTQREIVPQINPAKISQELANITQATKEDVEDAIKAAKDAKQKWLNTSFVDRSAIFLKAADLISTKYRYDMLAATILGQGKNVYQAEIDSVAELIDFFKFNVKYAQELYATQPSESSPGVWNRAEYRPLEGFVYAVTPFNFTAIAANLVGAPALVGNTVVWKPSKTAALSNYLLLTILEEAGLPSGVVNFVPGEPNLVTDVVLNDSEFSALHFTGSTRVFKDLYKKISDNVALDKYRDFPRIVGETGGKNFHLVHPSASVVHSALSTIRGAFEYQGQKCSATSRAYIASSVWDDFKTVLVEYLKGVEPLNTSSHEGLNGFMGPVIHEQSFDKISAAIESAKKDPELSIVTGGKYDKSRGLFIEPTIVQTTNPNHPFLKTEFFGPLLTTYVYKDAEYESILEKIDTTTTYGLTGAVFAKDRDAIRLAEEKLRYSAGNFYINDKSTGAVVGQQWFGGARASGTNDKAGSGNILSRFVSVRNIKENFYEISDYKYPSNYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWE4</accession>
    <name>A0A1E3NWE4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SAGA-associated factor 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_80796</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Functions as component of the transcription regulatory histone acetylation (HAT) complex SAGA. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction and promoter selectivity, interaction with transcription activators, and chromatin modification through histone acetylation and deubiquitination. SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). Involved in transcriptional regulation of a subset of SAGA-regulated genes. Within the SAGA complex, participates in a subcomplex, that specifically deubiquitinates histones H2B.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the 1.8 MDa SAGA transcription coactivator-HAT complex. SAGA is built of 5 distinct domains with specialized functions. Within the SAGA complex, SUS1, SGF11, SGF73 and UBP8 form an additional subcomplex of SAGA called the DUB module (deubiquitination module). Interacts directly with SGF73, SUS1 and UBP8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the SGF11 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036665.1">
        <property type="nucleotide sequence ID" value="XM_019185124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWE4"/>
    <dbReference type="STRING" id="683960.A0A1E3NWE4"/>
    <dbReference type="GeneID" id="30202370"/>
    <dbReference type="OrthoDB" id="3979720at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070461">
        <property type="term" value="C:SAGA-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013246">
        <property type="entry name" value="SAGA_su_Sgf11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08209">
        <property type="entry name" value="Sgf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU261113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ57458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="155" mass="17479" checksum="B8E166DD00750B74" modified="2017-01-18" version="1">MSENSNNEPPLTLSSMSLSIYRDIMETITREIFVETIMREQITRLRYGKLEAGNTPQPIETPIMNHSTINGNGSLLLSASNSVNNTNNNSRNPSPNPELLTRIEYYNVMGRDIFGNEKQSDSSRYFTCDNCSRKIAGNRFAAHIDRCLGGRSRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWP5</accession>
    <name>A0A1E3NWP5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_35133</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Mitochondrial inner membrane magnesium transporter required for mitochondrial magnesium homeostasis. Modulates the conductance of the MRS2 channel. Involved in the splicing of mRNA group II introns in mitochondria by affecting mitochondrial magnesium concentrations, which are critical for group II intron splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Forms homooligomers. Interacts with MRS2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Mitochondrion inner membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036637.1">
        <property type="nucleotide sequence ID" value="XM_019182272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWP5"/>
    <dbReference type="GeneID" id="30199518"/>
    <dbReference type="OrthoDB" id="10251508at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045016">
        <property type="term" value="P:mitochondrial magnesium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12823">
        <property type="entry name" value="Mrs2_Mfm1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000005">
        <property type="entry name" value="Inner membrane magnesium transporter MRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.330:FF:000002">
        <property type="entry name" value="Inner membrane magnesium transporter mrs2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039204">
        <property type="entry name" value="MRS2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890:SF0">
        <property type="entry name" value="MAGNESIUM TRANSPORTER MRS2 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890">
        <property type="entry name" value="RNA SPLICING PROTEIN MRS2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22099">
        <property type="entry name" value="MRS2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 14" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ57430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="405" mass="45758" checksum="1773D007E7FABEF4" modified="2017-01-18" version="1">MSYLRTNLVLKAFTKSSVFPSVARPIIKRSISNTSHLCLNNSITSRRNAEYTSLLLEKNLVHRNHLNPTEATVLRCTVFDGDGNLEAVSADLKRSDLISKHGLFPRDLRKIEKIGYNTEIAPSISVRKDSVVITLLHIRALIKADTVIIFDDLGSRNSHAQSQFIMDLESKLKAKNVDLPYEIRALEAVLVSAMTNLDAEMKVQTTVTQGILHELEDDITREKLRYLLIQNKKISTFSQKATLVRDVIDEILDNDDDLAGIYLTEKMNGTPRDVDDHAEVEMLLESYYKHCDEIVQTINNTISNVRTTEEIINIILDSNRNQLMLLSLRFSIGLLSMGAGLFIAAAYGMNLENFIEEQDYGLGAVIAVSMISIVVLFVYSLKHLNKLQKITIGDHYRQKMKDQVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWR6</accession>
    <name>A0A1E3NWR6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP synthase mitochondrial F1 complex assembly factor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_80859</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP12 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036732.1">
        <property type="nucleotide sequence ID" value="XM_019185133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWR6"/>
    <dbReference type="STRING" id="683960.A0A1E3NWR6"/>
    <dbReference type="GeneID" id="30202379"/>
    <dbReference type="OrthoDB" id="5322896at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3580.10">
        <property type="entry name" value="ATP12 ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2180.10">
        <property type="entry name" value="ATP12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011419">
        <property type="entry name" value="ATP12_ATP_synth-F1-assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042272">
        <property type="entry name" value="ATP12_ATP_synth-F1-assembly_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023335">
        <property type="entry name" value="ATP12_ortho_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21013:SF10">
        <property type="entry name" value="ATP SYNTHASE MITOCHONDRIAL F1 COMPLEX ASSEMBLY FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21013">
        <property type="entry name" value="ATP SYNTHASE MITOCHONDRIAL F1 COMPLEX ASSEMBLY FACTOR 2/ATP12 PROTEIN, MITOCHONDRIAL PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07542">
        <property type="entry name" value="ATP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160909">
        <property type="entry name" value="ATP12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ57525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="35773" checksum="C3441A2E0281A511" modified="2017-01-18" version="1">MLLFRRSVASVARSQLSIYRSYATTVGKNSIQHQTPSLENNQQTESNRLSKTLTKFWDQVKLSKVQDNLEIQLDSKTVRTPLGHTLSIPSDRKILAYLLLHEWASLSTLSIKTHSLPLTSLASRVIDLEHADKTQDLESQAKVGRREDIVEDLLRYLDTDTLLVFAPKSEYEGKLRITQEELYRPVISKIEELLGDDVKLTWLDTEVGLTGNKQDEKVRELARQFLLSLDHWDLVALEKVTLSAKSLICGILVLKAKSQGEEILNLEEIAKCATLEIVFQTEKWGEVEDTHDVDYQDIRRNIASAAILSFREDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWZ8</accession>
    <name>A0A1E3NWZ8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_35756</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036861.1">
        <property type="nucleotide sequence ID" value="XM_019182350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWZ8"/>
    <dbReference type="STRING" id="683960.A0A1E3NWZ8"/>
    <dbReference type="GeneID" id="30199596"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004032">
        <property type="term" value="F:aldose reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051268">
        <property type="term" value="F:alpha-keto amide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051269">
        <property type="term" value="F:alpha-ketoester reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016652">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042180">
        <property type="term" value="P:ketone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19120">
        <property type="entry name" value="AKR_AKR3C2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044494">
        <property type="entry name" value="AKR3C2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="20"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="4">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ57654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="297" mass="34058" checksum="8D14F15471B65F9D" modified="2017-01-18" version="1">MSLKFFTLANGNKIPAISIIGTGTQWYNASKDPTINEALVSQLKHALSIPGIVHIDAAENYSRYPELAKALKESNKPRDQIWITDKYSKFAETPIEHLNSSLKAIGVDYVDLYLIHDPFYENKKYDIVQAWRYAEQLYKEGKAKNIGVSNFAVEDLKKILEAAEIKPQVNQIEYNAFLQNQTPGIYKFAKENNILLEAYAPLGPLSERKGETGEFYDFLDELTKKYGKDQGQVLLRWVYQTGVLPVTTSSKPERITGSSQIWDFELTDDEVFKISKLGAQHKPLRQYWVPQYEKYDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX93</accession>
    <name>A0A1E3NX93_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">26S proteasome regulatory subunit 8 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_35396</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037026.1">
        <property type="nucleotide sequence ID" value="XM_019182301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX93"/>
    <dbReference type="STRING" id="683960.A0A1E3NX93"/>
    <dbReference type="GeneID" id="30199547"/>
    <dbReference type="OrthoDB" id="1664597at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045899">
        <property type="term" value="P:positive regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000006">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000044">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000030">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="172"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="18"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ57819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="396" mass="44164" checksum="7D8E16601AC27860" modified="2017-01-18" version="1">MAAVVHEGGIKPFLEQRIQDTEIQINAKTLNLRRLEAQRNSLNAKVRLLKDELKLLQEPGSYVGEVVKALSKKKILVKIHPEGKYIVNLSPEIDIKDIKPSLRVCLKSDSYTLHKILPNKVDPLVSLMMVEKVPDSTYDMVGGLTKQIKEIKEVIELPVKHPELFESLGIAQPKGVILYGPPGTGKTLLARAVAHHTECKFIRVSGSELVQKYIGEGSRMVRELFVMAREHAPSIIFMDEIDSIGSSRVEGSSGGDSEVQRTMLELLNQLDGFENSKDIKIIMATNRLDILDPALLRPGRIDRKIEFPPPTVAARAEILRIHSRAMNLTRGINLRKIAEKMNGCSGADVKGVCTEAGMYALRERRIHVTQEDFELAVAKVLNKNDEAQVSLAKLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXE5</accession>
    <name>A0A1E3NXE5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_97240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037035.1">
        <property type="nucleotide sequence ID" value="XM_019186579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXE5"/>
    <dbReference type="GeneID" id="30203825"/>
    <dbReference type="OrthoDB" id="4078936at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="218" mass="24565" checksum="F1F83BF6603725DB" modified="2017-01-18" version="1">MFSIGAKLYKPAGLRAFHSTSKVLSAINSIDGVIKVKEPFAIAATPIDPKYRAGYEPLYVAPSHKHIATMKRVSLAVGVVGIYVSHLMFDSVVVANELSYVVGALSTLPIPAVNYFTQNYVSRIFRLYDSTKPQTLENLVTDETLLAEKISWTGRSYYFRLVKVKDLKLSNDRFGWVNWKTDKDQFYVTDDIGGIKMDRIWGIVEHNSGVDNGRFFEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXH5</accession>
    <name>A0A1E3NXH5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">WICANDRAFT_64011</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CDC50/LEM3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037065.1">
        <property type="nucleotide sequence ID" value="XM_019183652.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3NXH5"/>
    <dbReference type="GeneID" id="30200898"/>
    <dbReference type="OrthoDB" id="1933107at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010607">
        <property type="term" value="P:negative regulation of cytoplasmic mRNA processing body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000749">
        <property type="term" value="P:response to pheromone triggering conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd20710">
        <property type="entry name" value="NOT1_connector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.840">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 1 TTP binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007196">
        <property type="entry name" value="CCR4-Not_Not1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005045">
        <property type="entry name" value="CDC50/LEM3_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055454">
        <property type="entry name" value="CNOT1-like_NOT1_connector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032191">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024557">
        <property type="entry name" value="CNOT1_dom_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032195">
        <property type="entry name" value="CNOT1_HEAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032193">
        <property type="entry name" value="CNOT1_TTP_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038535">
        <property type="entry name" value="CNOT1_TTP_bind_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040398">
        <property type="entry name" value="Not1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162:SF8">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25097">
        <property type="entry name" value="ARM_Cnot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03381">
        <property type="entry name" value="CDC50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16415">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16419">
        <property type="entry name" value="CNOT1_HEAT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16417">
        <property type="entry name" value="CNOT1_TTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12842">
        <property type="entry name" value="DUF3819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04054">
        <property type="entry name" value="Not1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="2148"/>
            <end position="2171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="2436"/>
            <end position="2458"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 HEAT repeat 1" evidence="17">
        <location>
            <begin position="248"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 TTP binding" evidence="16">
        <location>
            <begin position="662"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 CAF1-binding" evidence="15">
        <location>
            <begin position="868"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1" evidence="14">
        <location>
            <begin position="1181"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1-like NOT1 connector" evidence="18">
        <location>
            <begin position="1441"/>
            <end position="1594"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-Not complex component Not1 C-terminal" evidence="13">
        <location>
            <begin position="1757"/>
            <end position="2115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2478"/>
            <end position="2509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="2484"/>
            <end position="2495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="2496"/>
            <end position="2509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF25097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ57858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="2509" mass="284321" checksum="955B34FC86B0813B" modified="2017-01-18" version="1">MSISVSLSQQESNRPQEGSRQELLNSKVKEKIISQDLIKITSSQLASLATIINESNFDSTFDQIHKICNSSVPEVYYRYITRLLLTSSTLVETNTITNQFVEKLIAKELKYLVENPQYIDRFSNIVSRTDSVLLKQFTLKELFDHFKLDEFEQVSLSLSLENSQDLKAQVKSILEENLSSSINLLKTKNTNQTLVFFTSFFGSQLPSVYQKLLTFSSLLEFLAQASSEYPGLELAISKFFKSLSEMKFQEILATLMPEHIVPERLLNDLLSIQSQEDLDDALALLLSEILYPGSQNLANTPGLTALTPQNVPEASAKGSQFGSIIRKLPKQPTWNVIFSKMLSRLVNPVITQESLSELLSAFDDSTIDAFLEYQWPNDFKINLYISLRQLNPSLGAFDLLRTNYLKPIVSDLSYPNLKNSVLYYYNVTKLELDCISNSGAIENIEHKRFLHQIFEEDIRTAPELIALGCLQYSPPSPTIDDLLENLVVHLLDGSSSYYPLILKTLDQKDQLLKIVGKLITNKVSINDQIMAYLVSSDLISGYLESVDLANGLDLAVLSVKAGWEGFSSYLFKKKRDTSFNDVLLSYLRVQSSIDESKTNNQKTTSLKAIFTLLETVAKQDLSETQFQEFQSIQSQTLQAFPRLINFGFGHDDVILANGDVNTFPFDVEQQMKVFYQRMYNHEIDIKDVITMLSELRESNVPRDQDVFACMIHSLLDEYRFFPEYPLNALAITSVLFGSMIYFQLIRGTALTIALRYILDSCNQSPDSNMFKFAVQALYAFRQRLQEFPNYCSALSEVDSLKSQPQIYQIIINAVNNKSSGGDVPLNQTQKHQQIFASNTTAAAGGSEAKPQIFKSITAQVLFPNNITQEQPRQEVSQRILFLVNNMSDENLLSKAKEIQALLSPSIFHWFATYLVGQRAKTEPNNHVLYGSLVDVISNDLFVEYVTSVTLEEVSDLLNDSEETPSERSYLKNLGSWLGRITLGRNRPLKHRNLSLKDLLLEASEASKLALVIPFVCKILDQTKGSEVFEHPNPWTIGVLRVLAELYEYASLKLNLRFEIEVLCNSLKIDLKDLEPTLFIRTHGLPDPKATLSDGVVNMNLNDTTAPGLPQFTQPTNLPDAQQQVPTRDPASLMNQPQVQQQQQQQQIIPPQLGPQVAQPQQQINEALFQHLLGSTSFVTHPNLKRLFIMALTKSIREILPPAVERAVSIAVTTARSLILKDFATEVDELKLRSAAYTLVRRLAEGLALATCRDPLKENIQTTVQSFAPQLLNVENSPFEELPQAINDNIDLACSFVENAAMDKAVKEIDEVLVASIAARRQHQERRTDQPFYSQYLSRYSVQLPEPLGLKPTGVTPRQYQVYDAFGKHELTSPNAVTTQVIQPQLEAQALAPTSSVNNVNQQQQSQQPITIEQTFAYLQSLVDSLLKAIADVEGTKLDDIPSDSPIRLIITQIFSLTARNLAGESLMLKIAQFIVNALFTTSESDLSVEVFVFLLEKICTISVSTRKDVCWWLIHASDERKFNSKVMFTLLKAGLISASDLDRPLSKSITKENKNSIDFAIKLIKESVSSENSVAFRSDFALTVDVLRDIKDNENIVEFLNFLEKEPTSLVNNEVSESDRMGYVFTEWVRLLQHEPKNEKLHIVFIHQMVKSGILNKPESMTLFFRNAVEISVAFFKESDPVSDVFLSTDALSKLIVKLLILQQESEISRSNYFKLILSIISLVFANDHENSQSEFNERPYFRIFSTLLSEWSIVATDSHDLDDFNTSFYLILADFFNAYQPLAFPGFAFAWVTLISHRMFLPNIIESNHPKAQGKFVLLLLDLLRFETKYIKSKDMPEVISVIYKGTLRIFLLLLHDYPEILVQHHYQLCCEIPQSFVQLRNIVLSAFPKSISMPDPFTQGLKVDRLPEITEAPVISYNPSNDLRSLKKPVDGYLRIPSNMLLKTIINGIKQTPVEEAGIGYSKSNFNVKAINAIVLYVGIQAVNDRQPNGQTFNPKSSHFALLSGLMQEGTVEFQYHLIEAICNQLRYPNSHTQWFSYIILHFFGAQSLWNGKKSQVQQIITRVLLERVICNRPHPWGLLITFTELLKNTELAFFDLDFTKSAPELENIFGSLLRHIGSNSKKPPNTAFRQQRLKSWQPILSPRSVLPLLFLIAIIFTPLGIALIYTTFNVQQLVVEYSKCSELANTSDFGQVPSQYVSKHFQSYDSKDYNPPQWKLTQNDDDLTCQIKFSIPHEIKAPVFLYYKLTKFYQNHREYVESYDLKQLKGEAVSADDLDSDCKPLKTNSEGKPYYPCGLIANSFFNDTFQSPYKSDDESSVYDMTDKKISWSADRSKYKKTKYDPSDIVPPPNWAKRYPDGYTSENLPDLSQWEALQVWMRTAGLPSFMKLALRNDSQTLEKGDYVINIGLNFPVSMFGGTKSMVLTSSSVVGGRNLSLGIAYLVVAALSVFFGIIFLIKYIIQPRKLGDHSYLTFDQQDKNEQRTTTGSRSNLEPQDSRDEPRNVREIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXN7</accession>
    <name>A0A1E3NXN7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_80105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <location>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ57941.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="534" mass="58834" checksum="9CD8CFB6B07A6D24" modified="2017-01-18" version="1">MIQMKRQHLMKKHLMIQMKSLLEALDEELAGAELDSDEETTLDEEALDDSDDELAGAELDSDEETTLDEEALDDSDDELAGKELDSDEETTLDDSDEETTLDEEALDDSDEEALDDSDEELAGKELDSDEETTLDDSDEETTLDEEALDEELAGAELDSDEEALDDSDDELAGKELDSDEETTLDEEALDDSDEETALDDSDEETLDDELAGAELDSDEETTLDEEALDDSDEETALLAGAELDSDEETTLDEEALDDSDEETALVEEALDDSDEETALDDSDEETLDDELAGAELDSDEETTLDEEALDDSDEETALDEEALDDSDEETALDEEALDDSDEETLDDELAGEELDSDEETTLDEEAIDDSDEDTTLDDELAGAELDSDEETALDEEALDEELAGAELDSDEEALDDSDDELAGAELDSDEETALDEEALDDSDEETTLDEEALDDSDEELAGKELDSDEEQLDLEETTLDEELELAGCEDEEDEALVEEELEMHGFELDCDEDEDEDELEVDGINDFENDEVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
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        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="368"/>
            <end position="453"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF08573"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <sequence length="512" mass="58883" checksum="5DE1B96C11E0EC54" modified="2017-01-18" version="1">MSTEKESLTLVLKKAVALAEGLEQKSGVRSSINQQVAEQIRENKNLKEQLAVEKSKNEELVRRLKLEERKNQDYNRLNVKYNQTIKEKEQLEIELEKLKYKKRTPLSPSNNANTDNPGILGMLKQTTKPKQSTKKPESIYIDKENQQPLPKKPTTNISFSSSQNQDRTYESSDLAPTQFTMDGDELETHKPTYQKPRYQHYETFDAKDNIPIMEDSIDLRVPAIEIPPSSPTVKSEAVSQDLEEVHSSPIVTRNPIKVKKEETDSLEIVDSQDGSDDLNAHFPTPKTATKFQTPFNIPKSFDMKKSLLHTPDDLTPMKKTKKSKTMKSSITSSSTKKYDFTNNPKENREWIYEDFKIDPSKNDNYDYAYDAVLRGAERRCQHGKSCKNCENFYKIAKGDQIKPGFAGLQWNAESSKLNGNQTIISKSSRHRTIWDREASPPGFGDFDFPNTQQQKENKEKSLKIRRNKAYKRLFSALNNGKYLFKDNAYNEALKNNDYSIDESVFNKYITDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
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                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3NY25"/>
    <dbReference type="GeneID" id="30203783"/>
    <dbReference type="OrthoDB" id="5594612at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        </location>
    </feature>
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    </feature>
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        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="326"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ57477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="36120" checksum="91E52B2875762E1E" modified="2017-01-18" version="1" fragment="single">MNTYQSFSPSTLDLQTLVTKTDMSKTTEAYNDILAASEDYRKALLQVAEAAGNFGAALENGAKCKGSGNAADGLLSASGLYFLVANHQQILAHSVKESFEVPVSKEINKFKLTSAKNDEIFKNEIKEKVTNLKKQERENMKLSKLKSRNLVTYRSKLLQLTSHIDEIDRAKHDYYQSAFDLVQDTSLNILKQLGSIVRAQVEIYEGIARKGWSGGGLDELITGCPDPFTNEDDEDEDEEHAQNHNDIQFGSDNAIDNEDNDDDDDEQDDGDADDDDDDTIGPIPQLKEQLPLTSNTHGLVFNSHTGSVTEPILSHSHSTKSFEKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY33</accession>
    <name>A0A1E3NY33_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">DNA replication complex GINS protein PSF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_33463</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
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            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
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    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the GINS complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GINS1/PSF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037171.1">
        <property type="nucleotide sequence ID" value="XM_019182072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY33"/>
    <dbReference type="STRING" id="683960.A0A1E3NY33"/>
    <dbReference type="GeneID" id="30199318"/>
    <dbReference type="OrthoDB" id="10252587at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902983">
        <property type="term" value="P:DNA strand elongation involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11710">
        <property type="entry name" value="GINS_A_psf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21696">
        <property type="entry name" value="GINS_B_Psf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005339">
        <property type="entry name" value="GINS_Psf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056783">
        <property type="entry name" value="PSF1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12914:SF2">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12914">
        <property type="entry name" value="PARTNER OF SLD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24997">
        <property type="entry name" value="PSF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GINS subunit" evidence="7">
        <location>
            <begin position="56"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="DNA replication complex GINS protein PSF1 C-terminal" evidence="8">
        <location>
            <begin position="134"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="183" mass="21312" checksum="86D427F11D74D32A" modified="2017-01-18" version="1">MYGDHAAKLVNEAKRAQNLESLALFQDELIRSIVKETKDLDRQKDMLGQDEFSDRADQAQVFVTHLCMRRNKRCLLAYEKLRAQRLDQLAWENTEPELTNHNEIEYFKAYSDILIDYKSSFAEVDLSGSLVPPKDVFIDVRVIKDAGEIQTEYGVFNLTKDSQFFVRKADVERLIQQGYLQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYA9</accession>
    <name>A0A1E3NYA9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RTA1 like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_34224</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lipid-translocating exporter (LTE) (TC 9.A.26.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037292.1">
        <property type="nucleotide sequence ID" value="XM_019182166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYA9"/>
    <dbReference type="STRING" id="683960.A0A1E3NYA9"/>
    <dbReference type="GeneID" id="30199412"/>
    <dbReference type="OrthoDB" id="3358017at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007568">
        <property type="entry name" value="RTA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465:SF1">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04479">
        <property type="entry name" value="RTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="284" mass="32817" checksum="D46BF7A784005DB1" modified="2017-01-18" version="1">MESPQFSYYSYNPTQVGAIIFIVLFGILILWQTFQCVQSYIYILIPFYIGLFCEFIGFIARSISHDSIFDRSPFITQTLLILIGPPFFSASIYMIFGRMVTRILHNEEFLLLKAKYITKICVVGDVISLFLQAIGGIAISGAEDNIDRFNMGKNIIIGGLVIQILFFGFFVVVETTYYIKLRMNSDSSDYNLYVLNQDIKQFPYRFNNWKSILESLFVCSMFILIRSVYRVIEFTEGNGGYISSHEWFIYVFDGLMMFFCAVLFVSQDVSNYFLKTIPLTLGKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYM6</accession>
    <name>A0A1E3NYM6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Hexaprenyl pyrophosphate synthase, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_33708</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037425.1">
        <property type="nucleotide sequence ID" value="XM_019182107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYM6"/>
    <dbReference type="STRING" id="683960.A0A1E3NYM6"/>
    <dbReference type="GeneID" id="30199353"/>
    <dbReference type="OrthoDB" id="9927103at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032478">
        <property type="term" value="C:heterotetrameric polyprenyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097269">
        <property type="term" value="F:all-trans-decaprenyl-diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000010">
        <property type="term" value="F:heptaprenyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF69">
        <property type="entry name" value="ALL TRANS-POLYPRENYL-DIPHOSPHATE SYNTHASE PDSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="53276" checksum="1BCCF10A5DD6D026" modified="2017-01-18" version="1">MLRGLQRVKIRSAHNHSLRCFNRSKSSWTTAIETAAKLVTPSMILKDPISLVSHEMSQLASNIGTLIGSGHPTLNRISAYYFESQGKKIRPLLVLLLSRALSEIPIEERNRIKIDDLDIPEQPVYEGTKSLGAILKEQHSQGQISPLRILHGINPNIILDPLTKPHETLPEFDSKRGILPKQRRLAEIVEMIHTASLLHDDVIDNSDSRRNRPSGNIAFNNKMAVLAGDFLLGRASVSISRLRNPEVIELLSTSIANLVEGEFMQLKNTVFQPDEDLIDEDKLIPKPTGAIDTTVHEYSVSVPSGQGITHAQQVDAAFEYYIHKTYLKTAALLSKSCRSAAILSGANENVIDNCYQFGRNVGLCFQMVDDILDYTQTSESLGKPGNADLKLGLATAPILFAWRENPELGELIKRKFKHEGDVEIAMKEVYKHDGLTKTKQMAEDYCHRALENLRSVLPESDSRSALEFLTNAIVTRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYN7</accession>
    <name>A0A1E3NYN7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ketoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_20360</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037391.1">
        <property type="nucleotide sequence ID" value="XM_019180868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYN7"/>
    <dbReference type="STRING" id="683960.A0A1E3NYN7"/>
    <dbReference type="GeneID" id="30198114"/>
    <dbReference type="OrthoDB" id="417891at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF133">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="5">
        <location>
            <begin position="2"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="234" mass="25089" checksum="43D0FF761BC70E1A" modified="2017-01-18" version="1" fragment="single">GQTALITGGSRGLGLHIAKRLSQNGANIILLSRQGDILKHNIKHELSIISNNQQHHYIQCDLSQLDSIGECLSSSNILQKLNILINCAGISQNSLLFSTSSSSIQSIIDTNLTSSIILSQFLMKPLMRNSPSSIINISSVLGLKGFRGTSAYSASKAGLVGFTRALAVEMGSKGLRVNSISPGLIKETEMGQGLKFENVLNKDGVSMESVYQTVEYLLRNQSVTGQNLVVDNGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ20</accession>
    <name>A0A1E3NZ20_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Replication protein A C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_33850</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037678.1">
        <property type="nucleotide sequence ID" value="XM_019182118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ20"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ20"/>
    <dbReference type="GeneID" id="30199364"/>
    <dbReference type="OrthoDB" id="25571at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045184">
        <property type="term" value="P:establishment of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030491">
        <property type="term" value="P:heteroduplex formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04478">
        <property type="entry name" value="RPA2_DBD_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040260">
        <property type="entry name" value="RFA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014646">
        <property type="entry name" value="Rfa2/RPA32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014892">
        <property type="entry name" value="RPA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989">
        <property type="entry name" value="REPLICATION PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989:SF16">
        <property type="entry name" value="REPLICATION PROTEIN A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08784">
        <property type="entry name" value="RPA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036949">
        <property type="entry name" value="RPA32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OB" evidence="6">
        <location>
            <begin position="76"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A C-terminal" evidence="7">
        <location>
            <begin position="192"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="28442" checksum="105E26CC3AFF98DB" modified="2017-01-18" version="1">MNNIAYQPYGGYNQDYNGGDYNNTSGGFDSNGGGSQGTRTQTKNSLTPVTIKQINDSKPIIQDGEFIVHNLELNLVSFIGVIRKVTDNASNLQVQLEDGTGSIELRKWVDENAHDESNAFLQDGKYVYVTGSVKEFGGKKNIQHTTFREITDFNEVLYHHLSAIDCHLKAQGVKSETKPNESSNGLFVSGPAESEGGLSVADKIYEFINENTPSMPEGVPVQLIAQNLNLLVDDVVLHCGKLTEDAKIYAGYDENGFLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ52</accession>
    <name>A0A1E3NZ52_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_64423</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037491.1">
        <property type="nucleotide sequence ID" value="XM_019183732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ52"/>
    <dbReference type="GeneID" id="30200978"/>
    <dbReference type="OrthoDB" id="5408025at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027915">
        <property type="entry name" value="DUF4452"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39615">
        <property type="entry name" value="YALI0E17897P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39615:SF1">
        <property type="entry name" value="YALI0E17897P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14618">
        <property type="entry name" value="DUF4452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="209" mass="22570" checksum="93909480601B98D9" modified="2017-01-18" version="1">MSLPGQQSNSTLHQQQQQQTNGYDYSPLANQVYPYGGGSGSASSAGGSSSNGYVYNNSPLANNAVLNSQTNSSPYNNLNLSTRNNNLNGIYPTPRVSNLSPGLNFASLYSTRARFNASKGFDLEDDLEFCPEIHEHHTVQHHRFNPYTSHTFSPQNSPSASSAHTTSPVTKKDLGQSDTPKVITPRAKKVLEIVNPATGLRVASPAPYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZD7</accession>
    <name>A0A1E3NZD7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GTP-binding protein RHO4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_23116</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037591.1">
        <property type="nucleotide sequence ID" value="XM_019180975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZD7"/>
    <dbReference type="STRING" id="683960.A0A1E3NZD7"/>
    <dbReference type="GeneID" id="30198221"/>
    <dbReference type="OrthoDB" id="4031310at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030011">
        <property type="term" value="P:maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090338">
        <property type="term" value="P:positive regulation of formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000983">
        <property type="entry name" value="Rho family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ58384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="164" mass="18138" checksum="24EE5BAEE4EAB48E" modified="2017-01-18" version="1" fragment="single">KLVVVGDGGCGKTCLLISYSQGEFPTVYVPTIFENYVTNVKGPKNKIIELALWDTAGQEEYDRLRPLSYPDVNILLVCYAINSITSLHNVRETWTPEVKHFCPGIPIILVGTKMDLSSTSDSIVDFHEAERLAQEIGAVEHIRCSAKTTENINHVFNVAITSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZP2</accession>
    <name>A0A1E3NZP2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_84434</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037883.1">
        <property type="nucleotide sequence ID" value="XM_019185711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZP2"/>
    <dbReference type="STRING" id="683960.A0A1E3NZP2"/>
    <dbReference type="GeneID" id="30202957"/>
    <dbReference type="OrthoDB" id="783096at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32440:SF0">
        <property type="entry name" value="PHOSPHATASE DCR2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32440">
        <property type="entry name" value="PHOSPHATASE DCR2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="243"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="550" mass="63906" checksum="0F18B3BB8DC36225" modified="2017-01-18" version="1">MLLSYKFQRFFKRVLIPFAVLLYIVLSFFSLHTRNIYHNGLHQLEPYRGGPFDDIKKIKCYRWYGHCNQIIENSDYDNGDIIWHRVDKNLDLNGESPSSWSFYREYLYVHISTKKRFNSPGRAIVDLAIGDQSSKDPPFYVVNDAQSRSGRPKSRLDVTYNYNNWELRFDGIWAKYDKSNSEDAITNVQYLFGNDIIEPRADWTLVNGNINSNAKITVHRGPLNEKGDILKKVPILLQQNDEFKILQVSDLHFNSDFGTCKDQVNEEHGCKADAKTLSFLHQVLDLESPDLVVLTGDIIDGFNTMDYQTAILKALSPFISRSIPFAIALGENDKNQYASREEIINFIMELPYSMMGNNDPKQHHNSISGDKTNYALRIYDSKNDHLQNVIYILDIFQEDVNQQEFLKQTFDSFTEKPKFSLEFQHHPIQQYRPKSAFAIVGAYNEKSKLKIKTNPQIRQTLSDINVQAMSVGHEHTNECCIHSEDKDNKLKPLWLCYGGGTGEGGYGNTKIKYERRVRLFRMNTQKMEITSWKRKQSEPSSVFDYQYIYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZR0</accession>
    <name>A0A1E3NZR0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein MON2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_22139</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037314.1">
        <property type="nucleotide sequence ID" value="XM_019180929.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3NZR0"/>
    <dbReference type="GeneID" id="30198175"/>
    <dbReference type="OrthoDB" id="294853at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032817">
        <property type="entry name" value="Mon2_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16206">
        <property type="entry name" value="Mon2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Mon2/Sec7/BIG1-like dimerisation and cyclophilin-binding" evidence="5">
        <location>
            <begin position="4"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2/Sec7/BIG1-like HUS" evidence="3">
        <location>
            <begin position="202"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2 C-terminal" evidence="4">
        <location>
            <begin position="869"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ58107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1484" mass="168883" checksum="7D320474AAF9EB88" modified="2017-01-18" version="1" fragment="single">MSTVSHLSTELTNLVSESKRRNSEIKHASERSLTILKAFDGRSNEATFLKELSMNPDFITPFLLSCSTRNAKFSTIAIQCLNKLILSRAIPISKLDLLLDAFIEATHLAVDIQLKVLQTLPTLFQIYGEFINNGLVAKLLLICSMLQAPSKIPMVINTSAATQQQIVLSLFDKILEDDKTDVEKEFTVKIDEDEDLKISSSAFDAYNVLNDLCSLVEHQKPTFLTFKTMSESFGFELLENVLTNYQDVFLSHTELGFLVRTRITPLLLRSFSNHRDFLIIVRVSRIILLLIRTQISILEIEAEVMLSLLTHVISKESNVPYWKKVLSLEIFNAVLSDFDLVKDIFSSYDYHEDKKKIIHGFLSVCSEIVKEPWATRVLQINDIVLVPAQEHSITMANSALKIPYIDLLDKHEAPPAPKAYTLYLILSCTNSISEGIGNFIRKISTNDSNTFTFLNELSSENHEQSEALQMKTLVTSNAKSIVSIGAEFLYASLGNELFHALIRALQKLCHAAGVLGLNAERDSLLMLFSIATISNNLRRNSKSLSISETIVETISHSIAPSPASTPNIQRFHQRYLNSRHIICFRALVSLVISLGPTLKKSWKFILSTFQWFDYYLNGPSEYLSFKETPPKPELANTDLKSAESSFSKLFESTADYDDEAFRDGLIELISISRMSVFDKIDNSEPIIEDDILLCPYNRDFFIKKLVIVSKFNAKRYLEKGNKYWIDVVKFLSDVCTSPQVASDLRVTACATFNLSTKELAAVAFSPDSSFDKIYIETELLNSLAKMVDRMLSSLGETQLSTSESEMIHDTLKTLYELLDRFGTHLSHSWEAVIHIVDCPFKFFEVSKNVSSQKQLLKSAFEILQLVLNDFLQTIPLKIMIKIIDVLQKFCTQEYELNISFSAISYYWLISDYFRHLVPNEGGPELSFNNKEELLQALNNTDKYTEVHSLWLYLLSSIVNISNDPRPEVRNGAMQTFFRIIDSHGSYLNWNKTYSIVVKELLNIEFDNKLIEDPEIIASYKDSVSIILKGLTDLYCRFFIGSNEIIYWEGLLEFFQKLVKWNATEITFFVYKSFNEITASFKTIDVKIFDLLFEFWSSQNITYVVSDTDNYQNCIVELIGSFKSLYKLSDVSVTQLEKALSIFNNAIRFPFLPRFTKDIQKPTKVQAVVLECLEVVDISTDSEVLSLMLSHMASIILLPFQTRARIVKKLQGKGKIEVPSFIAVSNEALGWLKSTLGQIKDYSPLVPNKSFLKVFKNLLDAVQSKVDDQVHQENILEYGLWKNALSILLLLGSEVSPLLGQEELDESKTHIFSEVWDHIIDSIVVSLPVANDSRDESFNEDIYQKFKKSILPNIGQEVVPDIALEKLVSSVWKSSFLYENDEIEDYLLNKLNSPAEVTQSLLSTQLNDYTTEPFKVLPQQHFRTICLQDLFMFSDLHSAKRLATKTLPFLICRLVLSFKKFAGSQKLLYYAPSSSVQQKELDLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZW0</accession>
    <name>A0A1E3NZW0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_63236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037936.1">
        <property type="nucleotide sequence ID" value="XM_019183512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZW0"/>
    <dbReference type="STRING" id="683960.A0A1E3NZW0"/>
    <dbReference type="GeneID" id="30200758"/>
    <dbReference type="OrthoDB" id="2405700at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038910">
        <property type="entry name" value="Hua1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28031">
        <property type="entry name" value="PROLINE-RICH PROTEIN HUA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28031:SF1">
        <property type="entry name" value="PROLINE-RICH PROTEIN HUA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="205" mass="22400" checksum="CEFEBC55F3086D2B" modified="2017-01-18" version="1">MGKIEELPPSYDEAISQAPTGQLPQYNNSQQHPPPPPPPPNRPSSYSSSSHQIPQQQQPQVRPTNSVPWQYPRGYWCSKCHNTGKKLKNGKSCNKCWKRFAPRNGATTVNTNVGTQYSYSSNSSNFGGFGLPFSSGSTNVGFTHGPQSYSYQTTTVNNMPPRFVKPGDPAIGGIMCGNCRGSGLVRFFLDEDLCPVCHGVGRVFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZX4</accession>
    <name>A0A1E3NZX4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Geranylgeranyl transferase type-2 subunit alpha</fullName>
            <ecNumber evidence="2 9">2.5.1.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 9">Geranylgeranyl transferase type II subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_84504</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to cysteines occuring in specific C-terminal amino acid sequences.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.60"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the protein prenyltransferase subunit alpha family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.60" evidence="2 9"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037968.1">
        <property type="nucleotide sequence ID" value="XM_019185720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZX4"/>
    <dbReference type="STRING" id="683960.A0A1E3NZX4"/>
    <dbReference type="GeneID" id="30202966"/>
    <dbReference type="OrthoDB" id="1658at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097354">
        <property type="term" value="P:prenylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.120:FF:000035">
        <property type="entry name" value="Geranylgeranyl transferase type-2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.120">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002088">
        <property type="entry name" value="Prenyl_trans_a"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129:SF2">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-2 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE ALPHA SUBUNIT/RAB GERANYLGERANYL TRANSFERASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01239">
        <property type="entry name" value="PPTA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48439">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51147">
        <property type="entry name" value="PFTA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="336" mass="40646" checksum="E914B69E91A85B3F" modified="2017-01-18" version="1">MHNVKRQHFSEEVKKQRQAKDALKIRNYRSLQDKVLNLKNERVYSADVFNLTTDLLQLNPEFYTIWNYRRDIITNYYQKNSSEEDLAKLFDQELGFIMMRLKEFPKVYWIWGHRVWTLEHHPRVNWKAELMIVSKLLTMDSRNFHGWHYRRYVISKLEESTGESYALKEFDFTTEKINKDFSNFSAWHNRTKLIPILLESKPTSQFESSLGFLQQELTYLKNAMYTDPQDQSVWIYLRWLLTSDIFIEKLDRKTYIEILNQELTNVKELNQLEKEDNGVENNWCLKSIVTIETILNKIENNPTYKTEIEESLVKLIDIDPLRKNRYTYLSENYFEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZZ7</accession>
    <name>A0A1E3NZZ7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_63283</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037983.1">
        <property type="nucleotide sequence ID" value="XM_019183520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZZ7"/>
    <dbReference type="STRING" id="683960.A0A1E3NZZ7"/>
    <dbReference type="GeneID" id="30200766"/>
    <dbReference type="OrthoDB" id="5579088at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034389">
        <property type="term" value="P:lipid droplet organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019388">
        <property type="entry name" value="FIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23129">
        <property type="entry name" value="ACYL-COENZYME A DIPHOSPHATASE FITM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23129:SF0">
        <property type="entry name" value="ACYL-COENZYME A DIPHOSPHATASE FITM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10261">
        <property type="entry name" value="FIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="294" mass="33339" checksum="FE8B79E83849D3A8" modified="2017-01-18" version="1">MASRPIVHLIQNFHVAFLDKSFFLVIYPIVLSLGLLIRTLLSQELFDELKNSYWISDQNIINKTFADHGLEIFTTLSVLIFIVRIIVSELQNFKVPLLPTSFNSKNKTIEKIKKYSKLAVLYGARYGVTYGILLILFKLKNYESVSTGGQCQPRLQNSDDVSTLSKRYLKILCSPGEEWIPGSLNISGHYFFIVTLSLSLFYELKILLHSTMDSIDKSFEEQDDGLGLSNKLKVSIASATLTTLLISLVLLVWIGTLSVTAIFYHTIREKIMGLVFGYLAPVVNYVILARYYEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0G3</accession>
    <name>A0A1E3P0G3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Coiled-coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_24600</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC124 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038156.1">
        <property type="nucleotide sequence ID" value="XM_019181040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0G3"/>
    <dbReference type="STRING" id="683960.A0A1E3P0G3"/>
    <dbReference type="GeneID" id="30198286"/>
    <dbReference type="OrthoDB" id="76412at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010422">
        <property type="entry name" value="Ccdc124/Oxs1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054414">
        <property type="entry name" value="Ccdc124/Oxs1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21680">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21680:SF0">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06244">
        <property type="entry name" value="Ccdc124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coiled-coil" evidence="4">
        <location>
            <begin position="2"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="75"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ58949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="96" mass="10706" checksum="ACC0C3325B266CC3" modified="2017-01-18" version="1" fragment="single">SASGIDSALEALTLTKKAGAVSDKDIDRHPERRFKAAYAAFEEQRLPEARKENPGLRLQQVKQLLFKEFEKSPENPFNQATNLAHNATQEDVAALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1H0</accession>
    <name>A0A1E3P1H0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_84919</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019185807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1H0"/>
    <dbReference type="GeneID" id="30203053"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="56" mass="6551" checksum="AA1AADE26DB39EAA" modified="2017-01-18" version="1">MWLKAAFVAVKLDRELKPREIENQDPNQPEHQQQNKLTTVSKPSQSDRPIDMFTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P231</accession>
    <name>A0A1E3P231_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_33088</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038461.1">
        <property type="nucleotide sequence ID" value="XM_019182030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P231"/>
    <dbReference type="STRING" id="683960.A0A1E3P231"/>
    <dbReference type="GeneID" id="30199276"/>
    <dbReference type="OrthoDB" id="93019at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051178">
        <property type="entry name" value="TfdA_dioxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43779:SF2">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT XANTHINE DIOXYGENASE XAN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43779">
        <property type="entry name" value="DIOXYGENASE RV0097-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="11"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="377" mass="42329" checksum="A0A61235AEDA09FC" modified="2017-01-18" version="1">MPSATTTLKVEPLSGAQLGAKITLPQGITDPSKLSEDDFTKLNEALHKYSVLVIPNQADLPPNSQWKLTARFDPTTQTNGQNYGHGKEFRHDRSVLKKDGTSIPDQPQVQVLGQGEWEAGHHGLGDIKLTHPTHFTFHKDTLTPEQVEANQTRFYRWHIDSALYELSPPVATTLLGIHVPPHSYTQKIVYEDTKEELELTQGATAFVSGKNAFDILSDEDKELALNTKVVYAPHPYIFISEAKATSDGLTMVNEGKELPLDELPEWEESKVKKLPLVWTNPITGEHHLQAHACCVWRLERSNGEVLELEDARKELYRIMRPAISPKYVYAHSWEAGDLVIFHNRGVWHAVTGQFKPGERRLMHQCNIASGVDPVCAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P256</accession>
    <name>A0A1E3P256_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein arginine N-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_32833</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038537.1">
        <property type="nucleotide sequence ID" value="XM_019182009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P256"/>
    <dbReference type="STRING" id="683960.A0A1E3P256"/>
    <dbReference type="GeneID" id="30199255"/>
    <dbReference type="OrthoDB" id="1368803at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032174">
        <property type="term" value="C:cellular bud neck septin collar"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000144">
        <property type="term" value="C:cellular bud neck septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005824">
        <property type="term" value="C:outer plaque of spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035241">
        <property type="term" value="F:protein-arginine omega-N monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035243">
        <property type="term" value="F:protein-arginine omega-N symmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045840">
        <property type="term" value="P:positive regulation of mitotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007857">
        <property type="entry name" value="Arg_MeTrfase_PRMT5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035075">
        <property type="entry name" value="PRMT5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035248">
        <property type="entry name" value="PRMT5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035247">
        <property type="entry name" value="PRMT5_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10738">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10738:SF0">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05185">
        <property type="entry name" value="PRMT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17286">
        <property type="entry name" value="PRMT5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17285">
        <property type="entry name" value="PRMT5_TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015894">
        <property type="entry name" value="Skb1_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PRMT5 TIM barrel" evidence="10">
        <location>
            <begin position="36"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="PRMT5 arginine-N-methyltransferase" evidence="9">
        <location>
            <begin position="300"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="PRMT5 oligomerisation" evidence="11">
        <location>
            <begin position="466"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="433"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="442"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="334"/>
            <end position="335"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="417"/>
            <end position="418"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for specifying symmetric addition of methyl groups" evidence="7">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR015894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR015894-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR015894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR015894-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ59330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="658" mass="74721" checksum="D9EB85009696D544" modified="2017-01-18" version="1">MSNDLGSPYIGIKPTLSTKATAGKNIVLVEQYVETGYDYVLLAITNPNYREKSKKIFHRAKTSSDITVPSPNLNDVNIFAGSQISNTIGLLSSWIELESDDELIAEFSKQVLINELEYAKFIGLKHIIMAPPKDLLKLSNYSKSIYDALDITESHHITISISLPLCEDIEGLSSRPQHIDSLSTWDMWNSIRTNADYNPRLKVSLALPKSGIPITVVERWFAEPVSVLLMSSSIFILNNKGYPVLSKTNQSLLHHFHIKKPIYLLHGLDKIPEEVDPSAYMTYINYILVRNQPPMSKIDKVSSKHHDVLLPPLQPLATHLDDFTYSVFEEDNVKYDLYGKAIYCALSDLSHLNNINIAIVGAGKGGLVDAAFDAVTKLGINSRVNITAIEKNNSAVIYLSKRNFDNWNQSVDILNVDMRDWSPKESYNIIISELLGSMGCNELSPECLYPLEKCLDKVHGCFIPESYTSFIAPTFTPKLYNTLKSEGNEIAFHKQYVVKQLESCLCSTKINDIWTFHHPFSNFLKTHKKRTISTFKIRHKTVIHGISGFFTAKLYKDIEISIRPDNHTPDLISWFPLFFPLEQPLYVTDDTELEILMVRESSNGKVWYEWSIETFMYLVVPGESSNEFQMRVRTGVTKLHNHRGKGFSMDLNLNYLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P280</accession>
    <name>A0A1E3P280_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_101259</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038712.1">
        <property type="nucleotide sequence ID" value="XM_019180315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P280"/>
    <dbReference type="STRING" id="683960.A0A1E3P280"/>
    <dbReference type="GeneID" id="30197561"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF11">
        <property type="entry name" value="AMIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="5">
        <location>
            <begin position="73"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="3">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="227"/>
            <end position="230"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="539" mass="59368" checksum="90786E0E16CF0974" modified="2017-01-18" version="1">MTNDWKTLAEAKKKSVDELIPQEWRIPTEIKSNVLGLHETFGILNEKEIAITKNYKVKELLSKIASKELSSVEVATAFSKRAAISTQLTNCCTELLFKEAIERAKWLDDYLEENGQVFGPLHGLPISVKDSHFIKGVDSTVGYVSFIGNKAKITDDAAVVKILRDLGAVIHVKTNVPTTMMTGDSENNIFGRTLNPNNLKLTAGGSSGGEASLVKQRGSLIGIGTDIAGSIRIPSLSCGVYGIRPTAHRFPFEGNQSPTRENALCPISVVSGPIAHDLEDLNFVLDLILNHSNASKYCHDMLPIPWNSKIELPSTIKVGVVLECDSVPIQPPLKRIIKEAVSKLDKNSNFEIIYLKGFPSYKEGWATAFKLFISDPKDESIANIFKSGEPMINSLSKGGMEVYGPPPKIPDEIFELVSKKNEIKNKWIDLFNEHDLDVILAPGAPHTASPHDDFGIAPYTTVWNLVDFPAMVIPFGAVDKEIDQYDDVKIDYCPCTAPSYDKEVYHGGPGHVQLICRTLQDEKLLKIAELTDKVLNKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2H9</accession>
    <name>A0A1E3P2H9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_18623</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019180799.1"/>
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    <dbReference type="Proteomes" id="UP000094112">
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    </dbReference>
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        <property type="entry name" value="HODM_asu-like"/>
    </dbReference>
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        <property type="entry name" value="HODM_asu-like"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="314" mass="36926" checksum="B067E500835AC544" modified="2017-01-18" version="1" fragment="single">VDPNFNWSTKEPHKYRPFKKGEYKLVMGINNIPNDEWFLVENTYKSYTDLKFSYVNNPELHDHAIFMTQDCIPSVIEFYKESTRFMLERYPMCFSVRDGLIHNHIRGDTIPFNPLVEKDPKELLHHLSRFLEEDFILLFPDPENYDTDEYIFKGGVFAFAAGFDPVERFQTPLTTIHGPVPEYRTKLKPQMNKFFEKLKKGTIVKRNNWSIQTHNKIFVIGANKGVEGEEIKSLNPDELDFKRQVFFRSERQTLTRLPKTGTIVFGIRTYLTPIWDIREEGLGDELIGGIEGMQETIGQYKRRPEWGEPVVKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
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    <name>A0A1E3P2K5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">WICANDRAFT_31744</name>
    </gene>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ59137.1"/>
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    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004741">
        <property type="term" value="F:[pyruvate dehydrogenase (acetyl-transferring)]-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PP2C"/>
    </dbReference>
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        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
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        <property type="entry name" value="GM14286P"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="82"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="514" mass="57397" checksum="DA4EAA6F8712868A" modified="2017-01-18" version="1">MKDLNIALASGILFVASYISLSSHYLSDSGDPFRNFSTTQSNSTSKNSNKQNIFLLSEDEITRRLRNLEESYYVNRGKGVVRYDISQLPSNNPIEDDRSESVITVPTSESENADFYFWGIYDGHSGYHTSLKLRDELINHVVNELGAIYKSDPTNESIRLIPSQDAIDTAIKNGFLGLDYEIVQKSLQNLLEDPNNKEKAINALPAASGACGLLTFYDSSSHLLKVAVTGDSRALLGSLNDENEWTVKSLTVDQTGDNPEEVERIRGEHPDEQGVIRNGRVLGSLQPTRAFGDFRYKLKEIAGKSIESLPEHLKIYFRAPPKNFLTPPYVTAEPVITTTKINPSKNDFLVIGSDGLYELLSNEEIVGLVVKWLENHKINDNSPALNSKSWKLFGSNSDSEKKLPKVLDISDEKYKDFQRPAFRNKNNNQQHEYLLEDDNVATHLVRNALSLGGSKEYVSTLVSIPSPTSRKYRDDLTVTVVFFGEDGVIKEDGKLEVNLPATKGGKLTEQKPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2T0</accession>
    <name>A0A1E3P2T0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcriptional regulatory protein DEP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_68302</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038922.1">
        <property type="nucleotide sequence ID" value="XM_019184112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2T0"/>
    <dbReference type="STRING" id="683960.A0A1E3P2T0"/>
    <dbReference type="GeneID" id="30201358"/>
    <dbReference type="OrthoDB" id="20886at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013907">
        <property type="entry name" value="Sds3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21964">
        <property type="entry name" value="BREAST CANCER METASTASIS-SUPPRESSOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08598">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01401">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="145"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="156"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="201"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="224"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="250"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ59715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="481" mass="55630" checksum="C408AB4796E1591C" modified="2017-01-18" version="1">MTDIETIPEKRHIYSEPGETSPLSDLPTSPGIYKQMTTDQTPTSTNNNTTNEPASKATSTKPTEGRSKSIASDESSDLSDLDSEAETERMYDGAESQLEKIAKREDQDSTLLQEDEEEADNTVVKGDYIDQLDEDKDIEIEIEANEEFGKVVEQLKTSEEPEPKAESEPVKENGTVDEDVKKRKLSESLLEPIKRRKTADEEKEDTQEEQVDEVTKDEDVKDDNGDEANHEDEEPQPEQESQVGVKKSEAEDENVNEAEDEDVGEEEDEEEEEEEELTEEQKVEKQKELEVELEKQKQRKEAIQFLTEIEVDFAKLRDRLYEDKMSRFKSELEMCLNGSHPELQNVYTKIDSHREEKVRLASLSQKYKFECIDRKTRAQRTAIHQQYFKRVSDFKSGYLKELTEKWYKVNKERRLMDSLVPEYSYKVPEYTDELVTQRAQVNEEISVLVGLKEYYGFPRAPELAPIEGEELDNDLKAMNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2V2</accession>
    <name>A0A1E3P2V2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Autophagy-related protein 101</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_68398</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATG101 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039023.1">
        <property type="nucleotide sequence ID" value="XM_019184117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2V2"/>
    <dbReference type="STRING" id="683960.A0A1E3P2V2"/>
    <dbReference type="GeneID" id="30201363"/>
    <dbReference type="OrthoDB" id="10259639at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012445">
        <property type="entry name" value="ATG101"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13292">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13292:SF0">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07855">
        <property type="entry name" value="ATG101"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="146"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ59816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="294" mass="33274" checksum="08986CCB343A91CE" modified="2017-01-18" version="1">MEFSFDLTADRQHVHDAVKGLIWTIFFYRAFGSIIPSTAEFLDIPYPYFDSPELDNEIEQKLDHLLSRIDSDIATPTLASTSTTSTPFGSPGAKNFTLSSTATSSTNMISNPYANIKFNEPEPLTTFDEFDNDKHSSSVHSLGAISNKLSTKSPSSMPTTSSTLQTTSTRTATNNNNNNVKFGQFTIKFYERKKLKKPTWFGKTEEEINWETWILNVRVVQNNTNHQSTLLENTTNQFEKTVLEISETCDTNKNHIPVITSTELFPFPYTMSTSHGNNDSWKAFIKKMLKDPIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P324</accession>
    <name>A0A1E3P324_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_84893</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038499.1">
        <property type="nucleotide sequence ID" value="XM_019185802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P324"/>
    <dbReference type="GeneID" id="30203048"/>
    <dbReference type="OrthoDB" id="4036548at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="479"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="390"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="516"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="556" mass="59191" checksum="E44B51BEDEA7F844" modified="2017-01-18" version="1">MSNSTSSSTTLLGSTSTTILSSLSSSSSSSSSSEKQATSSSITSIVTPSLSALDNSTITDTSSSNSTLSSASSSDDSSARLFHTHDSSTLNSSSITSSSSISSSSLPSLSSSSEESSSFHLHSLPKLSTDSSSSSKTSSPSESSSITSTVFSSSPSSSVIPESTTSSPTYTEASSSASPTTTQGSTTFVYTQIYCLTDESTTITTALPVSTVLQDPSTFSYSSGSTKTTDLSYFANSIPGFRTSTSSSDDNSGSTKGKIAGSVVGSVAGVSIILFLLWFFVFRKKRFGGNHSEKGSFTHSITSTRRLDDEYGFDDSHNYDYSEKPQHSRPSFLSGVLSKFKKPATNTSQDHYYHSQIQHHDTPPQDTQDLDGLGSLRSSILRGQQIPNERTPITSTMTTNNTIPTTRPSHYQSSSSPSSSSYPQNNTQLRTIDTVYEETESENSTISRPNHRLSSRNLGNSARYQNAGLTNYMLQNPFDESIGIKKPPPPPPPPKRRANSYNVVDANNRFSVESNGSSLTEDTDSSSTDESLGSIQMGDGGDARDDQRAGFFKEIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P344</accession>
    <name>A0A1E3P344_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anaphase-promoting complex subunit 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cyclosome subunit 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_83756</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the APC5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038877.1">
        <property type="nucleotide sequence ID" value="XM_019185591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P344"/>
    <dbReference type="STRING" id="683960.A0A1E3P344"/>
    <dbReference type="GeneID" id="30202837"/>
    <dbReference type="OrthoDB" id="2504561at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037679">
        <property type="entry name" value="Apc5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026000">
        <property type="entry name" value="Apc5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830:SF9">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12862">
        <property type="entry name" value="ANAPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 5" evidence="9">
        <location>
            <begin position="282"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="776" mass="88310" checksum="78081858D749AB2C" modified="2017-01-18" version="1">MEQSSTKVVLTPSFAPYKIVLLTLIAAYCAGILPKKTHRPLLTTIVKYIERPSIYHDTESSVNVPTLDEILRNIKESCLKADPVEGVSYAHDIEQRLLHALWSLKSLDSLHSFINRARSMLTKNLKDASLFMNSKPAGSVPKYLLTRSSFLGNFVTTCIASFETLEFDKSELLWTSFVNFRISSKSKWVKKHAERIPDVHDFFGTADKDDDFSQLKSVFNYLPLNENEIREEIVLVSQEDLSKLLDHQVHILETYGTPTSPELRRTLALMTQAETGKLPSIHYIKYLESLRSADYEGAFYSLHRYFDYMMSNRRTVFYHYALLCLATLHAAFDCEQEAIRAIEESISVARENKDYTCLNFVLSWLFNFMKDRPTIKHDFYLSNTHLLQFLKTNSGLTSSSLHSTAYQSEATQIMMEGGSTSSVLESLTKAAHISLSEDSSLVSFISYCALASAFWFRVGNYDLAKAYCEISLESSTSISQKVSIFIRKASVEYSLGNVEEAFIILEKQKSLVCSDLRLSKELVSHKLILLSRQCIRSSRCRMAKYYLEKLEAQHHVNIDIESELQYLTACLHLRLGNLQLAFDIVSKQTSTYARKASNKFWFLKFSLLHCEIMSQSSTPARAFSSTIKCIQSASKSGFTLVVLEGVLKLADILMKLNKLEDARGLLSDCIPYFEQLSDLALKSKAYQVIAEGLISLFKTEDIDRKKKIQQLTKIVDHLEKSIDGFKKLSMFQEIKKCLELQISIGKKINHEEMLNHAQSSLVSIDLKIKEELAIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P355</accession>
    <name>A0A1E3P355_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_78538</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039116.1">
        <property type="nucleotide sequence ID" value="XM_019184743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P355"/>
    <dbReference type="GeneID" id="30201989"/>
    <dbReference type="OrthoDB" id="5232040at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133666" evidence="1">
        <location>
            <begin position="19"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="143" mass="16031" checksum="3F8ECFDD21EE4A96" modified="2017-01-18" version="1">MLKSFVFFAAVLAPSIFALPEPQTSVVRDGQYANYTLSYGTDKIISSKAIFDSRGKWKEDSNVELYDIKYTDSFSDTDISFDGSSFTANWKIEDTNQLVSIYFLAKTTDGSDFAQDVELSITGKYKECDECDAYIALPRSIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P398</accession>
    <name>A0A1E3P398_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_83917</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039102.1">
        <property type="nucleotide sequence ID" value="XM_019185617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P398"/>
    <dbReference type="STRING" id="683960.A0A1E3P398"/>
    <dbReference type="GeneID" id="30202863"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17327">
        <property type="entry name" value="MFS_FEN2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000064">
        <property type="entry name" value="MFS allantoate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF1">
        <property type="entry name" value="ALLANTOATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="350"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="381"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="441"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="56"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="503" mass="55853" checksum="A3B031FE634B46E3" modified="2017-01-18" version="1">MTEFKEKATETIEVQSLPVSQKSEVDAAFEIALLAQDLEITDEINKKLLRKIDFYIQPIMWSIYTLQFMDKVTNGFAGVMGIQKDLGLVGDEFSWLVTGFYLAYLVAEFPISQSLQRLPFVKTLSACIIIWGIVLCAHAGAHNSAQIITARVFLGLFESSITPGFVILSSQWYKKEEQFARIAYWFSCNGAGSILGGAIGYGLLHHADSLGIESWRVLFIVTGLLTVVFGVLFYFYVPENPSKAWFLTEEEKLYQVQRIKTNQQGFGTKKFKKYQAIEAFKDIRTWIYIAWGLTAQIPNGGLGSFSSILLNRTLGYSKEKALLMGLPQGAIQIASGIITGFIATRSGKRVLIGVSCCAITLIGLCLLAFSDSPKVQLGGYYIIPFFAIGIVTILASIASDAAGHTKKLTVSAAFLVSYCVGNVIGPQTFRAKDAPLYQPARVTMAACLVVATVDLFLLLLVNIYENKKRDKKFKKDPNYYIAPENSEFLDLTDFENKNFRYSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3C1</accession>
    <name>A0A1E3P3C1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="19">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">WICANDRAFT_30895</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 19">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Membrane</location>
            <topology evidence="19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="19"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039122.1">
        <property type="nucleotide sequence ID" value="XM_019181778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3C1"/>
    <dbReference type="STRING" id="683960.A0A1E3P3C1"/>
    <dbReference type="GeneID" id="30199024"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990530">
        <property type="term" value="C:Cdc50p-Drs2p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140345">
        <property type="term" value="F:phosphatidylcholine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090555">
        <property type="term" value="F:phosphatidylethanolamine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140346">
        <property type="term" value="F:phosphatidylserine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090556">
        <property type="term" value="F:phosphatidylserine floppase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000026">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000047">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000010">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 18 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="380"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="430"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="948"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="975"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1016"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1058"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1087"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1126"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="23">
        <location>
            <begin position="119"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="22">
        <location>
            <begin position="213"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="24">
        <location>
            <begin position="908"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="702"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="27"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="62"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="16">
        <location>
            <position position="494"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="494"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="494"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="495"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="496"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="496"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="587"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="628"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="651"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="684"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="764"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="765"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="766"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="856"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="862"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="882"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="885"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="886"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="886"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049128"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051303"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="ODQ59915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1236" mass="139772" checksum="57B36FF0BA314231" modified="2017-01-18" version="1">MNLDDPFNDKYIEQDLDDSNDPFRDPGSSSNNRPTGLFSNVRQKLGLGNEYQDVGIPLTDYPSHDDPFRDPTSTQRDDRPPPDRDLNEDERNMFDIRRLYDKIRGRKRSINVDKGPRIIHLNDKTSNAAFGYGDNHVSTTKYNFATFLPKFLFQEFSKYANLFFLFTSVIQQVPNVSPTNRYTTIGTLMVVLLVSALKEILEDLKRANSDKELNSSKVEVLSPDHGEFITKKWINVAVGDIVSVKSEEAIPADLILLTSSEPEGLCYIETANLDGETNLKIKQAREETCHLVSPTDLIAMRGKILSEQPNSSLYTYEGNLNLYNKEYPLSPEQMILRGATLRNTAWIHGIVVFTGHETKLMRNATATPIKRTAVERIINLQIVALFGMLIVLALISSIGNVIQITADAKHLKYLYLEGHSKAGLFFKDLLTYWILFSNLVPISLFVTVEVIKYYQAYMIASDLDMYHAETDTPTVVRTSSLVEELGQIEYIFSDKTGTLTRNIMEFKSCSIAGHCYIDDIPEDKHAKIIDGVEIGFHDFKKLQDNLATGEDAMIIDEFLLLLAACHTVIPEVQSDGSIKYQAASPDEGALVQGGADLGYKFTVRKPKSVGIEIHGQYQEYELLHVCEFNSTRKRMSAIFRFPDGSIKLFCKGADTVILERLGEDNPYVSATTRQLEDYAAEGLRTLCIASRTVPNDEYESWVKTYEAAATDLNDRAQKLDDAAELIEKDLFLLGATAIEDKLQDGVPDTIHTLQDAGIKIWVLTGDRQETAINIGMSCKLLSEDMNLLIVNEDSVEATRENLISKLRAIREYKISQNEIDTLALVIDGKSLSYALDECDDLLLELGCLCKAVICCRVSPLQKALVVKMVKKKKKALLLAIGDGANDVSMIQAAHVGVGISGMEGMQAARSADFAIGQFKYLRKLLLVHGAWSYQRISQAILYSFYKNIALYMTQFWFVFLNCFSGQSIMESWTLTFYNVFFTVLPPFVIGVFDQFVSARLLDQYPQLYQLGQKGTFFNVTIFWGWVGNGFFHSAVIFVGSILFYRFDNALHGGQTADHWTWGTAIYTASVLTVLGKAALITNSWTKFTLIAIPGSFAFWLVFFPVYSTVAPLVNVSKEYVGVLAHLYTSATFWAMVFVLPVLCLLRDFAWKYYKRMYYPESYHYVQEIQKYNTANYRPRIEQFQKAIRKVRQVSRMRKQRGFAFSQDEGGQEKLIRMYDTTRARGQYGEMRDAELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3J6</accession>
    <name>A0A1E3P3J6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alcohol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_30689</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039256.1">
        <property type="nucleotide sequence ID" value="XM_019181753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3J6"/>
    <dbReference type="STRING" id="683960.A0A1E3P3J6"/>
    <dbReference type="GeneID" id="30198999"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000947">
        <property type="term" value="P:amino acid catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08297">
        <property type="entry name" value="CAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000002">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="10">
        <location>
            <begin position="40"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="372" mass="39755" checksum="919C614EE6074780" modified="2017-01-18" version="1">MLKTFRFNSNPTLLRYQALRGLATTSIPTTQKAIVFETNGGPLEYKDIPVPKPKPNEILVNVKYSGVCHTDLHAWKGDWPLPTKLPLVGGHEGAGIVVAKGDQVKNFEIGDYAGIKWLNGSCMTCEYCQNAFESNCAKADLSGYTHDGSFQQYATADAVQAAQIPKGTDLAQISPILCAGITVYKALKTANLKAGDWVAISGAGGGLGSLAVQYAKAMGYRVLGIDVGADKGELAKSLGAEEYVDFKASKNVVEEIQEKTKGGPQGVINVSVSDAAISQSTQYVRPTGSVVLVGLPPKSVIHSDVFSHVVKSISIKGSYVGNRADSREAIEFFTRGLVHSPIKVVGLSELPKVYELMEQGKILGRYVVDTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3N4</accession>
    <name>A0A1E3P3N4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_62666</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRN7/TAF1B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039308.1">
        <property type="nucleotide sequence ID" value="XM_019183413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3N4"/>
    <dbReference type="STRING" id="683960.A0A1E3P3N4"/>
    <dbReference type="GeneID" id="30200659"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070860">
        <property type="term" value="C:RNA polymerase I core factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001164">
        <property type="term" value="F:RNA polymerase I core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048538">
        <property type="entry name" value="Rrn7_cyclin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048540">
        <property type="entry name" value="Rrn7_cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033599">
        <property type="entry name" value="TAF1B/Rrn7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021752">
        <property type="entry name" value="TF_Rrn7_Zf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31576">
        <property type="entry name" value="TATA BOX-BINDING PROTEIN-ASSOCIATED FACTOR RNA POLYMERASE I SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31576:SF2">
        <property type="entry name" value="TATA BOX-BINDING PROTEIN-ASSOCIATED FACTOR RNA POLYMERASE I SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20645">
        <property type="entry name" value="Rrn7_cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20644">
        <property type="entry name" value="Rrn7_cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11781">
        <property type="entry name" value="Zn_ribbon_RRN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRN7-type" evidence="11">
        <location>
            <begin position="5"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Rrn7/TAF1B N-terminal cyclin" evidence="12">
        <location>
            <begin position="97"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Rrn7/TAF1B C-terminal cyclin" evidence="13">
        <location>
            <begin position="254"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="141"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="165"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
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            <end position="462"/>
        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006899"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF11781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20644"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF20645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ60101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="529" mass="61180" checksum="E7A9356C21E32BD9" modified="2017-01-18" version="1">MSNSWIRGPRCGVDNCRSKFYRSVDGRRICQFGHVNEGHIEFNDDDDDNFIVTRRLNIPTVTTGLSQASQITPGLQKDEAKRKLYGAAGRELYYKCFQCVLKSQIKSLINDHGLPEILEVVVKRYYVQYISMLNDIDTVEKSNGKLDDDDDEEEEEHGEYRDSEGFTTQDTSFTNDTSNEDHEFGFTKLPTHIDSLALCYLGCRYLNLPIFINDFVQWTTTNRIPYMRASFNVPKVLRDKLSMGKVRSFEPKRPPLKGDLCFAISKVATSLNLNHLELNYEPLLFKAIKELILSPQIYFATKKFLKLKLIKLDIKPFKGTKTAQSYINEFPEIKLISIIISITKLYFINGSVNENQPTYDWKKWKELLGNLDLDQDDDNKNFHNVLTQLLLSKNSSLEVIDWDEEKTNKYLDWFTHKIVNTQATEEGPAAMKRLFDIFKLPEDDSQEENLQQQPVQQPQSSEFTGKDVKSIESAYTQLIESGSNKEKLSDEEVMELEKFMIENLISNFGVTTSQLTHAVRHVEQKFFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3X7</accession>
    <name>A0A1E3P3X7_WICAA</name>
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        <recommendedName>
            <fullName evidence="6">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_84023</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="GeneID" id="30202882"/>
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    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HD"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
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        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="220"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
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            <end position="284"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
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        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
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            <begin position="137"/>
            <end position="230"/>
        </location>
    </feature>
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            <begin position="1"/>
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        </location>
    </feature>
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        </location>
    </feature>
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            <end position="195"/>
        </location>
    </feature>
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    </feature>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="302" mass="34384" checksum="22D7E9D5904B5ECF" modified="2017-01-18" version="1">MSISNESNTSVSSASTPTSTTTPSHSTSITSSAESSADEKGRFLLLHKEMIYNKDAPNNLSLPSMDHVLKNIESYQNEQQLSDSQLLLEFNQRNSQSSEHVQTSPEQDSNTISQYQQQYTFQQRSESTNSIEAFQNELKQEQQQQHHQPIPHHSLSQPQIPTTKSQQRPSHIHRNSTSNAITSLKRSTTPLSINTMDDDYDSRDSSVDSGLDSPNLTPNGSNNKKRTNLPRETIEILNEWILNNMDNPYPNHTQKRLLLEQTGLSNVQLSNWFINKRRRRLFSNLSGNNGQPIKKKRLIDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P425</accession>
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        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL54</fullName>
        </recommendedName>
    </protein>
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        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
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        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the mitochondrion-specific ribosomal protein mL54 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P425"/>
    <dbReference type="STRING" id="683960.A0A1E3P425"/>
    <dbReference type="GeneID" id="30197885"/>
    <dbReference type="OrthoDB" id="10252718at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013870">
        <property type="entry name" value="Ribosomal_mL54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28595">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L54, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28595:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08561">
        <property type="entry name" value="Ribosomal_L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="76" mass="8876" checksum="4BA232BC15CFE000" modified="2017-01-18" version="1" fragment="single">IKSSCPVGTLLNLKIKKSGAEPVALEDHEYPEWLWTVLDPKAQEEKLKADPAKYQKKLMRQRNRKNIKHNNFMAQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P459</accession>
    <name>A0A1E3P459_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like protein ATG12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Autophagy-related protein 12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_91432</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATG12 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038874.1">
        <property type="nucleotide sequence ID" value="XM_019186298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P459"/>
    <dbReference type="STRING" id="683960.A0A1E3P459"/>
    <dbReference type="GeneID" id="30203544"/>
    <dbReference type="OrthoDB" id="10003551at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Atg12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04110">
        <property type="entry name" value="APG12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="71" mass="7892" checksum="9CD26ECF77CE7D69" modified="2017-01-18" version="1" fragment="single">MLKSKITTSASVMLTRLPPNSSKVIKEVTKETNFKITIRCQPIGSAPQLNPTVFKISDTQPFATLIKFINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P461</accession>
    <name>A0A1E3P461_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Urea active transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_31202</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039412.1">
        <property type="nucleotide sequence ID" value="XM_019181806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P461"/>
    <dbReference type="STRING" id="683960.A0A1E3P461"/>
    <dbReference type="GeneID" id="30199052"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015489">
        <property type="term" value="F:putrescine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11476">
        <property type="entry name" value="SLC5sbd_DUR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031155">
        <property type="entry name" value="DUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154:SF4">
        <property type="entry name" value="UREA ACTIVE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="288"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="454"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="493"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="559"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="591"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="659" mass="71114" checksum="7F96950CAB3FF1D1" modified="2017-01-18" version="1">MTSATILSQSYGYGFVVGLGAAFAIVMCGITYVLAKYLNQVQNSERFTTASRNVGSGLISSAVVSSWVWPATILTSSLWGYTYGISGGYLYGMSGTFHILAFALLAIEIKRIAPACHTIGEIIKVRYDKATHFVYMFYVSATNLMVASCLLLGASQGFNAATGMNVIAANFLIPLSVAVYTMFGGIKATFLSDWIHTVIIYIVILIMCFKVLASSDIIGSPGKLYDMILESEAKFPSKTGTSFLSFKSKTMFMTTWSVNIGGFASVFGDPSYGQKAIAAEPKSVLAGYVLGGVCWLTVPWALGIIGGLTARALLVSDKFYTYPNELTSHEASAGLPLIYAMGTIMGTPGAVAAILDLFLSVTSALSAELIAFSSISTFDIYRGYIKPTATGKDLIRVSHAMTMIFSLVVACVSVIFNYVGVTVGWLIGFYGILLSPAASILVFTLYWKKTSSFAITYGSPISTICGVICWVATAYASGGAVNKNTLMTSEPSIVGNFVSFGGSWIIIPILSYLKPEKIEFDFKTYSTAFDAADDADEEEKENMTVSVSDQKALAKASKIALILTVLILIGGYMIVPLTFYGTDYKFSKKYFTQWVIIMSICLLLASIYIIVVPLYQGFESFKLIFQGVFRSKKADVIEGTGSDSEVIEVQLSDANNKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P463</accession>
    <name>A0A1E3P463_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_84015</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039247.1">
        <property type="nucleotide sequence ID" value="XM_019185634.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P463"/>
    <dbReference type="GeneID" id="30202880"/>
    <dbReference type="OrthoDB" id="3981174at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12191">
        <property type="entry name" value="gal11_coact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2920">
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="2"/>
            <end position="83"/>
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            <begin position="1201"/>
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            <begin position="1103"/>
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            <begin position="1218"/>
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            <dbReference type="ARBA" id="ARBA00009807"/>
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            <dbReference type="ARBA" id="ARBA00019613"/>
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        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
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    <sequence length="1284" mass="141954" checksum="BB5D6F2A4142A3AC" modified="2017-01-18" version="1">MDWRNELTNNERLRYVGVLVQALKELALTANNNNPAAFDVNKLKTSAGEFEKYCYQNASNRISYVHFFKQKILSIKNSIQQQKLQKQQQPGGVGANTTGNIGVSNIQLGNNTNSNNNANIPNQQNQQLGGTPNLNAGAPTPNQSQLFLAQQAQARQAAQQMRNQTPLQGANANVPGQQQQQNLQNAGLNAGLNYNNQIPQQQQQQRGNQTAPSQQQQQVQNSTNNSAQAALNVNMKTVPIPMALLQRIPNLPPNVNTWQQIFELVASRQLDSEYIGIIRQVYTIHTQLIQKQQQQQKQFGSNQQAPSTTAPTTGANMGVNAQIARQQEELRNRQLQQQQQQQQQQQQNQMPQQQQQQQQQQQQLQHNNANPQQQPQQQSAIPQLTPDLLQRLTENALQLLTKLQQEKQIPDNLTNEQRNMFIKRYISRQQQLRMEAYTKQQQQQQGANNGGAQNLNQQVVNPTGATQSRPVNQAQLQARLQNANNLAQQNQQQASQNQPNQQQQRWNEQGNNNNNNSNSNNNTTNNNVQMKLNNLPTGLTAEQQQQLNARRQQALYQQQLQQQQQQQAARGANLNTNVNQTVPGAANSNAVGGAGAGAVQNPILKNNVLVPTEQDRVELRMILNEVSKVPIKLTNLTAQLTPEQKSVVSSKVHSLLLPLFNLIEQLVPTFYTLTKNVDGTKKLLHMRFMVKEVLDGLRRNEYYASPELLEKVKAQSQKYVSFVKEQISRKNAAAAQAGGANANVNTGNANNAAINNNNNNFNINPQITPQQLLQRKQQQQQQASQLNPQITPQQLLQQQQIGTQPSQFINNNNNNNNNNAEFNPLELTNIPGSSSNNNNNNNGNFQNVDMSMINGGRLNTPDLSQMNVTPKLNQMNISGVAPSPAMGNANFGTTPNFSGSPQMMNAGVFNNNNNQQQPQPQPQPLVQGTFTQQPPAPQPIQPQQSSTPAASTPTTKKAPAKRKSTKGRKTSNASTPTLAPKSVPAPTAANGPTPGATGVSAPTGQATINNNSTPTTTLANVGGAAPGTIAPTALQAQMLKKEQEMISKEDQNKKQFEENKAKRMESYHTKPIDFFAASLANLLELKDEESKILNDSKKKTNGDFKSSPAQKGLTPSTILQTPLTFSNAAKTPNSTTKGGIPNGVSNASTYLSPNLSPSSTLKNVNGDVSTKWGPRVSREAIQKTFGNVEEIKKLLFVDEQENSNGEESGPMIPTPPPDNEKQSKEQKDSVDDDRKFKKIKIDDDNRFYDENDNQLNSDLMWDFDYVSSHIDDDDSTQPDFYINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4G0</accession>
    <name>A0A1E3P4G0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RecF/RecN/SMC N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_54232</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC6 subfamily.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_019182734.1"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019789">
        <property type="term" value="F:SUMO transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051304">
        <property type="term" value="P:chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990683">
        <property type="term" value="P:DNA double-strand break attachment to nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:resolution of DNA recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306:SF6">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecF/RecN/SMC N-terminal" evidence="13">
        <location>
            <begin position="13"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="181"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="368"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="630"/>
            <end position="664"/>
        </location>
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        <location>
            <begin position="720"/>
            <end position="754"/>
        </location>
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            <begin position="801"/>
            <end position="848"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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            <dbReference type="ARBA" id="ARBA00004286"/>
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            <dbReference type="ARBA" id="ARBA00006793"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022454"/>
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            <dbReference type="ARBA" id="ARBA00022741"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ60361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1039" mass="119127" checksum="5BA2EF1E11E218FE" modified="2017-01-18" version="1">MTQADPTKVAGVIKRVSLKNFMCHENFVLEFGPRLNFIIGHNGSGKSAILTGISICLGAKASETNRGSSLKDLIKEGANSCHIQVVLANEGDEAFEPGKYGSEIIIERVLRRDVQSAPYTLRSENGKKVSQKKADLDAILDYHNIAVNNPMAFLSQDAARSFLTASTDEQKYKFFMRGTLMDEIHQNIKKSRDQVDSMELKIIRMKEALLRLKDDARDAKKLHDRLISSNDLRERQRILHGKYFWMQADKAQDIVNEVRESQLEIQSTIEQLDRYIKDYESKIANFDISKEDLRFKKEHAETVYNDALASAGSKKSERDVFGHKVEATVRKMKETEKLIRDNKMRIADIDKDIRKEEDKIRERNGGSREALENEFENLKGRVNEIEGEKQQISENLASADSHKELYNDLQSQIDEKHQVMRNLQGYIKNAKSSKVDELAPYKDPVKRVVNDVKRGRFHGPVLGPVGTFLSVKQGFEDWASLVESRIGPNLNSFIVENVSDQRQLQSIFRKHHVRANIVLRKPEEFNYSSGIPDRRYTTVLDTLEFSNPAVKFALIDTAKIESIVLIEDRVEAQRVLESHTPNVQMIISKKDKYTGQVSSINRNGAFRVDPLYIDGSQLPKLRPKDRSQNAQVMENRLKGEQAQLRDLEAQKDQITQNINAERAHFTAKKRNLEIEEKRIKSRIYNIQQKLNDDADSGKLEGLKEDRASFEDEIKVYGATTGELQEEILKLKEQLKQKGDEYEELKAASKAAKDEVYSIQKKIMDFDSEKELNITKLQHFEKSKQKRLEEAQAMTENLPAWEKNIEENILQAEESCEKQEADSIRLDSVEAVQDAIEKVTAQVEKANEDLGKTPEQIVNDDAAAREKYAEAKAQFDNAVANKTYYHSSLNDRLKAFRESRGVTFLEADMDFKQSLQFRNFTGNLDFDVNKNKLTMYVSTKNDKKPRHVDSLSGGEKSFSQISLLLATWKPMRSRIKGLDEFDVFMDQVNRKIGMRLMLDKLSKENSQTIFITPQDIGQIAELDERFVRIHRIRDPRAARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4J9</accession>
    <name>A0A1E3P4J9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Biogenesis of lysosome-related organelles complex 1 subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_62372</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038994.1">
        <property type="nucleotide sequence ID" value="XM_019183351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4J9"/>
    <dbReference type="GeneID" id="30200597"/>
    <dbReference type="OrthoDB" id="3980145at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028119">
        <property type="entry name" value="Snapin/Pallidin/Snn1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14712">
        <property type="entry name" value="Snapin_Pallidin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="90" mass="10317" checksum="2DDF69D2987868E5" modified="2017-01-18" version="1">MELVVQSLLGGPLEGLSNHLQTLHESQVVLIARLKILEEKLRKYSDQLTIDIDVKGTNERLHIVKKKLTKIQSTLKIVEDRFEKLSTNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4P6</accession>
    <name>A0A1E3P4P6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Luciferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_62943</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the NtaA/SnaA/DszA monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039592.1">
        <property type="nucleotide sequence ID" value="XM_019183462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4P6"/>
    <dbReference type="STRING" id="683960.A0A1E3P4P6"/>
    <dbReference type="GeneID" id="30200708"/>
    <dbReference type="OrthoDB" id="3979273at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051260">
        <property type="entry name" value="Diverse_substr_monoxygenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016215">
        <property type="entry name" value="NTA_MOA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011">
        <property type="entry name" value="ALKANESULFONATE MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011:SF16">
        <property type="entry name" value="C2H2 FINGER DOMAIN TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000337">
        <property type="entry name" value="NTA_MOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="6">
        <location>
            <begin position="35"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="470" mass="52515" checksum="6A77892ACAC34CDF" modified="2017-01-18" version="1">MTGQISQARRNRGPLILSAATASTPRNWSLPNDTSREFTRDLNQSIEFAKLAEKAKFHNVFFVDHLTWFDVYGDSHEGSAKYGVNAPRIDPSLLVSALASHTKSIGFITTLSTISEHPYHFARRLASLDHLSDGRAGWNIVASYLPSVGKNLLDGAPIPEHSERYVKTEEYLDAVYELLLSSWRDDALGKYFSIKGPAITEPTKQRFPLVVQAGSSEKGIEFAAENAELVFIDTKSTDKIPEIRKLAETRFNRDPYSIKFITSLLPFIGNTHQEALDKFEEYKKAENLEANLVFFGGISGFDLSKYGWDEEVGDQGESNAIKSITKTVLNGDKGGQTKTDIAKKYGTRLSFAGTASEVADQIEDLLSKADIDGFNFIVSPYPGMLIDIVELLVPELQRRGLAQTEYSVPGGTLRENFYGEEGKTFLSGDHPAYNLRWRKGVSKEQFEKELSLYKALRNERRKSNIGKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4R4</accession>
    <name>A0A1E3P4R4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_105241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039477.1">
        <property type="nucleotide sequence ID" value="XM_019180441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4R4"/>
    <dbReference type="GeneID" id="30197687"/>
    <dbReference type="OrthoDB" id="10629736at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="280" mass="31998" checksum="55F0546C862F6ECA" modified="2017-01-18" version="1">MNDGERQQRPAKRSTPISKLVPKSDNELNIKSSKQTRSENGKVKRPISKEALQSFHTPNKHRNVQSIKRSKVIPNMPDTPVKKLLLSELASTPLTFKIHNDQSTQKNNTPKIPISDITPKSHVPKTTSNNIEKVPLQTILDQAQVQPFVEIPMMKTESSNFTSLDLTHDPNSLLYQAQLPIVHLNNWYRVTQHQIVERQTQITPTHEVISFQKFNGQVLLVDCIKISDNTKETALLLNYRQHPLASSKVQTIQQGSQISLNEGYKVFQSLRLYKNWKLQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4X3</accession>
    <name>A0A1E3P4X3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ran-specific GTPase-activating protein 30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_84213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019185667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4X3"/>
    <dbReference type="GeneID" id="30202913"/>
    <dbReference type="OrthoDB" id="512915at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030695">
        <property type="term" value="F:GTPase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008812">
        <property type="entry name" value="Ran_GTP-bd-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31010:SF2">
        <property type="entry name" value="RAN-SPECIFIC GTPASE-ACTIVATING PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31010">
        <property type="entry name" value="RAN-SPECIFIC GTPASE-ACTIVATING PROTEIN 30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05508">
        <property type="entry name" value="Ran-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="449"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="480" mass="54550" checksum="A7E50C8543030172" modified="2017-01-18" version="1">MDEILSKAGSQAVTFAIRSGISIASTYAVKSVTKFLAKVPDDQRIRLERTKQKLQTRIEIVSSAIDLIKLVASRGNTNLESTLRLTKDLKEEIDRFDDKIQEVSENYTSSKKDNQSVNNVELYMLDLLSRIEEAIPLINLSLTTSGANLSGTLSNQVSPGRLLQASNHVNKSNEKFKGELLQIGPTFELTLYSIFYHRGRIINNRLNEISWKEEFTRADISVWRQPSAIDEYQYVLKIKENFNDDRYHDDEDEPQVMELDISTITRLFFSASGKLLKLEDRSTPVLVLKINKNFQDIESDEDEDAQHESNPIEWLSFGEYEQLYDGSDESEDSDEEDSPKSAKSSIQQPLKPHNSLSLLEYILRLCVLQSNDQLSILEVNDERLALYLNDENNISDITSKLKKIEVNDDKEPSTPQKNGDNKNGDSPLPGEVESNVKLTPWERDKISKLSRTPLASSAGNQYGTPINTLRKKVLRRNLND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4Z8</accession>
    <name>A0A1E3P4Z8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pro-apoptotic serine protease NMA111</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Pro-apoptotic serine protease nma111</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_82938</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1C family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039746.1">
        <property type="nucleotide sequence ID" value="XM_019185464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4Z8"/>
    <dbReference type="STRING" id="683960.A0A1E3P4Z8"/>
    <dbReference type="GeneID" id="30202710"/>
    <dbReference type="OrthoDB" id="4217619at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120174">
        <property type="term" value="P:stress-induced homeostatically regulated protein degradation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06786">
        <property type="entry name" value="cpPDZ1_ScNma111-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06719">
        <property type="entry name" value="PDZ2-4_Nma111p-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025926">
        <property type="entry name" value="PDZ-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46366">
        <property type="entry name" value="PRO-APOPTOTIC SERINE PROTEASE NMA111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46366:SF8">
        <property type="entry name" value="PRO-APOPTOTIC SERINE PROTEASE NMA111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12812">
        <property type="entry name" value="PDZ_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="PDZ" evidence="8">
        <location>
            <begin position="271"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="983" mass="109006" checksum="DD0CC38B751E9C4C" modified="2017-01-18" version="1">MSLKRNLSSGNLNGSNQNKRLNNLPVQENGQDDEISVDQDISIDSPGSSDTFTDGKWHDTISKVVKSVVSIHFAQTASFDCDPAIVSEATGFVVDAERGIILTNRHVVGAGPFWGYAIFDNHEEAVVKPIYRDPVHDFGFLKFDPSSIKYMKVQSLELRPELAKVGCEIRVVGNDAGEKLSILAGFISRIDRNAPEYGGLTYNDFNTEYIQAAASASGGSSGSPVVDVNGYAVALQAGGSTEASTDFFLPVYRVLRALKCIQSQTPVTRGTIQVQWMLRPFEECRRLGLSDESEKKAREKFPEIIGLLVAEVILPEGPADGKIKEGDTLISINGIDISNFIKVDEILDASVEKEIEIILQRGGENVVVNCTVGDLHAISPNRYLEVAGASFNDMSYQMARIYAIPVRGVFINNATGSFFLDKTETTGWVVDSVNDHDITNLENFIEVMKTIPDRSRVAMGFRHISDLHSPHNSTIYVDRHWCTSFRLATRNDETGLWDYQELGDPLPALPPTPRSVKFIDIPMEQPGCAQLSKSLVLVTSTIHIPVDAFPAIRKRQHGLVIDAEKGLVIVSRNVVPHDCLDVHIVVADSLIIPASVEFLHPTQNFAVIKYDPSLIQTEVLTPKFSETPLKRGDKAFFIGYNYNSRVVTSETKVTDIASINVPRNVLSPRYRGSNLESVAFDTNLCTQCGSGVLADADGTIRALWLSFLGEGGKDSDVVYKMGIDITEFNTFIKDFREGRVPKVKIVDAEFFGLSLVQARIRGVSDEWIKQVENSGKDRLQFLAVSRVSVPEAGEPKTNFETGDIVLSIDGKPVYGITDITAQSTKDKLAFKIVRKRQELEIEVPTVEIKPTNHLVFWCGAVLQAPHHAVRQSMMNLPSQVYVTNRMHGSPARHYGISSTNFITHVNGIPTPTLEEFVKVVRQIGDNTYCKLRLVSFDNIPFAISIKTNYHYFPTGELKRDSNLSKWLDIEYNEENKETKVTHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5B3</accession>
    <name>A0A1E3P5B3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-processing factor 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_29761</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039677.1">
        <property type="nucleotide sequence ID" value="XM_019181646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5B3"/>
    <dbReference type="STRING" id="683960.A0A1E3P5B3"/>
    <dbReference type="GeneID" id="30198892"/>
    <dbReference type="OrthoDB" id="10257301at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000386">
        <property type="term" value="F:second spliceosomal transesterification activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000389">
        <property type="term" value="P:mRNA 3'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000348">
        <property type="term" value="P:mRNA branch site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000034">
        <property type="entry name" value="Pre-mRNA-processing factor 17, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032847">
        <property type="entry name" value="PRPF17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43979">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43979:SF1">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="2 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="205"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="249"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="333"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="432"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="93"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="137"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="497" mass="56600" checksum="4C0FD657546056EC" modified="2017-01-18" version="1">MPLINGYSSSEDEPEEIPKQVFKAQRVDAAPQVSAKPTSTALTITTIDHGDNGNKQIQKTISGHFERETFDDTTFEIQHRNFENLGYAKNKKRIKSKRAKKGKSDVVDGEHSYAGPWAKYHDSSSDEEQDVPKSASEESEAENEQFSETGEEDEEESETTEYFGESEIDYQGRSYMHIPTDVGVNLKNDAIPEECFVPKRQIHVWKGHVNGTNKILFFPNSNHLLLSCGNDSKIYLWSVYHKRELLRGFYGHTKPIKDISFNSDGTKFLSASYDHWVKLWETESGKCLAKFRLKSVANVVKFNPFNDDEFIVGLMNSKIDHYSIKTNQVIQSYDHHLGSINSITFLETKRFISTSEDKTARVWDLQINIPVKLISDPSLHSMPVTRVHPHNKYFAAQSMDNTIMVFSARDRYKTNKKKSFIGHNCAGYGIGIDFSPDGKNLVSGDSNGNALFWDWKTTKMIKKLKIDEKAITQVLWNTKEVSKVAFSGSSGKIYYYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5M3</accession>
    <name>A0A1E3P5M3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin-like protein ARP6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_77423</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039956.1">
        <property type="nucleotide sequence ID" value="XM_019184556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5M3"/>
    <dbReference type="STRING" id="683960.A0A1E3P5M3"/>
    <dbReference type="GeneID" id="30201802"/>
    <dbReference type="OrthoDB" id="337660at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="43935" checksum="1205CE347494AC5E" modified="2017-01-18" version="1">MQSYQYYPVIIQIGARFIRAGFAGDATPILRTPTNAYDLSDSQLNNNIEFKHALPEKDHIQKQDGLENNYLWTYDLTEFNAAKLECLLERIIYDIYQRNLLVDAKKCKVLVLEPPLFPIPLKKIIAKVFLFHIHAQSLRFYPEPVLSCVSAGSSSGLVIDLGWNQTTVIPIFDLRMMYKDIKVSCKAGRQLHEYVRDKLKEFESWAEVDSFSMTEKLICDTFYCRTKAILRTDAKEFEFENIIIPNSIRYELLERFMFKLEDSDLDNENHQLVPMITTTLRNLPIDLRSELSTKIIITGGLANIPGLKTRILEEVEQSLGHPVEAIDSLGSWEGASLYCSTSLMSPSTTGSKKSDELFRDKYLNGESVVTDWTDQLYTQQRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5X2</accession>
    <name>A0A1E3P5X2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Cytidine deaminase</fullName>
            <ecNumber evidence="4 13">3.5.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 13">Cytidine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_83004</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13">This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2'-deoxycytidine + H2O + H(+) = 2'-deoxyuridine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13433"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15698"/>
            <dbReference type="ChEBI" id="CHEBI:16450"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>cytidine + H2O + H(+) = uridine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16069"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12 13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.5" evidence="4 13"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039851.1">
        <property type="nucleotide sequence ID" value="XM_019185472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5X2"/>
    <dbReference type="STRING" id="683960.A0A1E3P5X2"/>
    <dbReference type="GeneID" id="30202718"/>
    <dbReference type="OrthoDB" id="414540at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004126">
        <property type="term" value="F:cytidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006216">
        <property type="term" value="P:cytidine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006217">
        <property type="term" value="P:deoxycytidine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008655">
        <property type="term" value="P:pyrimidine-containing compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01283">
        <property type="entry name" value="cytidine_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000008">
        <property type="entry name" value="Cytidine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050202">
        <property type="entry name" value="Cyt/Deoxycyt_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006262">
        <property type="entry name" value="Cyt_deam_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01354">
        <property type="entry name" value="cyt_deam_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004064">
        <property type="entry name" value="PRK05578.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11644">
        <property type="entry name" value="CYTIDINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11644:SF2">
        <property type="entry name" value="CYTIDINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="14">
        <location>
            <begin position="15"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="56"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR606262-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606262-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606262-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ60644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="144" mass="16100" checksum="B6C40101B18189DC" modified="2017-01-18" version="1">MPPAIQHDHKGLTRQEFEELKTKALEARDLSYSPYSKFRVGCCILTNSGKYFQGANVENASYGASICAERTTICSAIIQKEKSFKAIGISSDLEEVITPCGICRQFIREFGKTIPIYMFKSNGDFIKMSLEEILPLSFGPEQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P613</accession>
    <name>A0A1E3P613_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycosyltransferase family 15 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_15527</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040010.1">
        <property type="nucleotide sequence ID" value="XM_019180648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P613"/>
    <dbReference type="STRING" id="683960.A0A1E3P613"/>
    <dbReference type="GeneID" id="30197894"/>
    <dbReference type="OrthoDB" id="3979768at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF11">
        <property type="entry name" value="MANNOSYLTRANSFERASE KTR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="319" mass="37736" checksum="2E0424B889E0F764" modified="2017-01-18" version="1" fragment="single">RENGVILVFARNIDLYKLTETMWSVEQRFNKKYKYNWLIVSDALHNRNFKATVLSLSSGNVEFARVPRLNYPPNIDKARMKDSRKAFRQQSIVFRKTIKMRHQQRFWAKDILFIEQLAKYDYFMRIDPGVLLYCDVNFDFFKFMTEHQRTYGFGFAMKGKQGTFPSLWDDTLAYIKENPKDIHENNMIDFISDDKGETFNTCQFRANFEVGDLRFFRSDKYKKLAEYLDNKNGIYYEGWDESTFHTLGVALLEDRHKIQFFNNIGYNDRASDNLKSCPFDESIRLGSNCVCNPLDDLTWNSNAGSCIDKFFHVNSFQVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P643</accession>
    <name>A0A1E3P643_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_78921</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039524.1">
        <property type="nucleotide sequence ID" value="XM_019184807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P643"/>
    <dbReference type="STRING" id="683960.A0A1E3P643"/>
    <dbReference type="GeneID" id="30202053"/>
    <dbReference type="OrthoDB" id="1924787at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001080">
        <property type="term" value="P:nitrogen catabolite activation of transcription from RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN DAL81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="30"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="646" mass="74892" checksum="3C81B39E092C55DE" modified="2017-01-18" version="1">MSKRKFEPEDNKLFNVFTFSSRRVPSGRKSCSFCREKKVKCVNSEDNDERCKQCKTRDLSCSLCPAKAASTNHSSPSTVESPAVSNPQSSPVDFNINNGAQFLDLIGDMDWINVLDEELLQSDFPLAEAISINQNPGSTTRISGYNADTNGDGIPIDKRHGDTSFMYYGFSGEYDPFLRFNYYSYDDNDECFFKQKFFRKLGDSEMPAVFSFKTKKLLREQTKEYSTPGFDKKMIKKLYKCAPNLAVLYMKFIFPFLPILDGDFLGKIFSNETVEFNDISGGSPLFTLLLAKIGKGWERHDTSMSIFFDDQELPILKNRNELFTVAWNLLQDELHTPDLTTVKSLILFFQIISFKKYSQSNSFERTVLSNLVSVCFTLGLHVDCSDWKIPDDEKALRKRLWWTVFIMEKWNNFVVGTPSLINEKSYNVEIPSDINELFEDVSQYFSDINSKKFFLKLIELTFILNDTDEKLFSFQSVPKEEKPDAIRGFEEKLSIWYDGFKEIESASSVPFHSITLSYFVLKLILFRSRFQIYGVNKFKDICFDDAKVLLRDIVKFLRNLPSLVIHGFWYNWSRSLFMYIKDFSLLLVVISSTKEQSLEVLNIIKDFREWLEMNSKTLCLLWTTLLRINTATSSIGTQFKNSLKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P655</accession>
    <name>A0A1E3P655_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_84211</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039534.1">
        <property type="nucleotide sequence ID" value="XM_019185665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P655"/>
    <dbReference type="STRING" id="683960.A0A1E3P655"/>
    <dbReference type="GeneID" id="30202911"/>
    <dbReference type="OrthoDB" id="2123952at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF37">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="18"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="90"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="708"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="90"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="724"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="738"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="755"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ60327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="843" mass="94991" checksum="2E2FD19490011194" modified="2017-01-18" version="1">MSDQDQNPPLKRQKIKKACDNCRSRKIKCDGEQPCVKCRNSGANCLYTHVEKKRQVTKKDDKSSTIQSLDQRMNKMESLITTLIEKLNTPDGSSSYKDSTDGAQSSINLSASSTDINSPQNTEVQSQVLPSTSPVPPADDIGTAGLTTKSVNKFEDKYIGSQSSLSVLSPRGLMWLAKKANDPTIISQFKTIVTKSHGVFYNYMKKWIEPIDKSQVCQLPPRETMDQLMKVYEKDLLQFSFFMPLDEVRTMIDTYYGIQEKTTHKRLSNSEFLVLHCVAAISANLLAEEHAGDLTTLRTLKKIEDDNVNTAIHYYHRIAIIGEGIKSVQGILLLFSHTNFSLLPQANFTLISTAIRHSQGIGLHRKEALLGLPEEEKRKKRKVWWWAYLADRTDCLKAGKPLSIGDFDVSVMDLEEFRDLIFEGYSEELLNNIFSGEVDLMDPKAKGLTNLKEPSLPFDINAVISYFITCFYKNTGEAYEKLFSATALIGKNADQIMETIETLNLKLEAWNENVPISIRPGAPLRLGVYNDLVDDKLLMLHFTYYLHVMIINRMAFKRSWLNHDQETVDDNSTILPRQRKSIIKCLEAARTILRIVQQMNACKSSNFNIPLFVFTSAFFTLLTACLEFPTANESKNDLILIQHTIKVLFTKLQIMSNQLGDDKIYNIMAHSTKFFLRVGILVYNNANVDKIDSSALDEELRQYQKLLEESTSSHKRRRSGNLKPSSQFSPPQPSSLSHMRSTNTEQGVPQYGPSIPSSQANTPNLNNILNDPLSHATGQPYDGVSNPASVGSVSSTDGFDPFNLSEIDIAANNNVFKQLFPMPNLYFNGTDQGNDSLPVHFEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P682</accession>
    <name>A0A1E3P682_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Glycosyltransferase family 71 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_77592</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040133.1">
        <property type="nucleotide sequence ID" value="XM_019184578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P682"/>
    <dbReference type="STRING" id="683960.A0A1E3P682"/>
    <dbReference type="GeneID" id="30201824"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="term" value="P:protein O-linked glycosylation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392:SF1">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="588" mass="67797" checksum="659F67BE9CFE5533" modified="2017-01-18" version="1">MLVRSFNPRLNTILFLVIVLLFYYNAVELFKTSSSNQLVVPHKAAHISVLSILSKFPTQASPSKNCKKLWNEIRQLKIPEYNDTNFDPENFDPFFKKLINWEVQNNTALIRSYNHCFLDQNICDVELEKDLYPWFTFESPTINSGPKTKNRKLFKKLKCFKDIKKNLKGKGIVLTATEFHFKELVSLFTLLRALGNPLPIQIVHKGDLSDFVQDNLIKVATVPFDEKLKLKIGANSNAKLPKLKVEFINVSGVIVPKYDYRFKRFQNKLLAYLFNSFKEVIIMDVDSVPFIPLQDFFNIEEYKKTGAFFFKDRLMHLGHSMPDTLDFFKELLPTALEEDKLNISRATKHTLNNNFFNGFSHMMESGLVIVDRSRHFSNSLMAISLAQWTRAIMSRIWGDKELFWLSMSISGDENYSFNENGAASVGQISPIPNSHYPQQLELKELCSNHPGHVYKDNSLLWMNSGFKYCKKVMHVDEFNPFFKGWKLEDSNAFNDSPLKIRHALVPPIPNLPGKPFYPESDKPKYPWEGKPYCEGYTVCGYDKVGNDVGNVINFSQKETQQFDFLGQVYSKGMDVLIEVAGSDPKFDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6A0</accession>
    <name>A0A1E3P6A0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_84323</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039679.1">
        <property type="nucleotide sequence ID" value="XM_019185689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6A0"/>
    <dbReference type="STRING" id="683960.A0A1E3P6A0"/>
    <dbReference type="GeneID" id="30202935"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF15">
        <property type="entry name" value="GENERAL AMINO ACID PERMEASE AGP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="364"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="435"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="33"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="536" mass="60105" checksum="A4BEC16F64F608F8" modified="2017-01-18" version="1">MEKFDITSKEKEVDDSKQVEERISKRKLLPRQVSLIGIGGAIGTGLFVSIGTQLTHGGGASLLIAFCFWTTVFMGASNSIQELCTFLPVSGSFVHYTERFVDESCGFAIGWVYYLTQNANLCFELTAICLVIEFWTSKVPKAVWISVFLILYVLLNCFSLFMFGEGEFYLSMGKVILAIGLIIFTIVVMSGGNPEHKVLGFHNFAHKPFVEYLDTGSMGKFHGFMACLIFATYVFWGIDYLSLAAQNAIDPRKTMPRSFNKVLARLVIFYIGGALCVGILLPSDDQTLLDAISEHAGNAAASPYTAACVNLKVSVLPHIINALILTSIVSAGNSSLFSATKALQRLALSGKAPKIFAYTTRRGVPIYCVGMVLIISLLAYLNVSNGTNKVLTWFLNVETAGMLIVYIFICISYLRYRAGLKAQGFNRDEFIPYKGWGLPFFTWWSLFWFSLMLLMDGYTVFLKGNWDIQSFVFSYFMIAFFIVLFVGHKIFYRKGFIRASEMDFVSDIAELAEEDEYWRTHGYKLTKFDKAFGKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6A9</accession>
    <name>A0A1E3P6A9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH CENPB-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_61511</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040158.1">
        <property type="nucleotide sequence ID" value="XM_019183190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6A9"/>
    <dbReference type="STRING" id="683960.A0A1E3P6A9"/>
    <dbReference type="GeneID" id="30200436"/>
    <dbReference type="OrthoDB" id="2507562at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006600">
        <property type="entry name" value="HTH_CenpB_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03221">
        <property type="entry name" value="HTH_Tnp_Tc5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51253">
        <property type="entry name" value="HTH_CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH CENPB-type" evidence="4">
        <location>
            <begin position="93"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="166"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="173"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="189"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ60951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="377" mass="43405" checksum="412964D9587D69F2" modified="2017-01-18" version="1">MSMLMSTPQCLNNTSGIATNSYKVSKPKTKPRTTLEEKIMILNHYHQSNKPQADTVELFKNRYSISTSSFSEWLKDEESLRNLYNNSNEFLKKSKRKLRFKYDKINEEMDKLVEERIQNKKPINEPFLRENWAIFANRYGVEDPKRLLSFSHGWLSQFKKRHGLDKQSRMRKLSTTSLSSPNCDAASITSDTSSSTSSTDTLVETSNNTNITNELENMSGINPMKPSISTQSFLNLDVNEDYLQTNINNYENLINNASNNFMPIQISNTPTLLQSASLSRVNSRPLQSNSIMNSSMTNLLNFANTSTTSSSTNNIEYDLINEGDFEIFLNKYGEDFLGFNQDKYPESFKILGDLKDVFKKEKEGYNDKKLRELLFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6D6</accession>
    <name>A0A1E3P6D6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondrial 15S rRNA processing factor CCM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_61568</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Regulates mitochondrial small subunit maturation by controlling 15S rRNA 5'-end processing. Localizes to the 5' precursor of the 15S rRNA in a position that is subsequently occupied by mS47 in the mature yeast mtSSU. Uses structure and sequence-specific RNA recognition, binding to a single-stranded region of the precursor and specifically recognizing bases -6 to -1. The exchange of Ccm1 for mS47 is coupled to the irreversible removal of precursor rRNA that is accompanied by conformational changes of the mitoribosomal proteins uS5m and mS26. These conformational changes signal completion of 5'-end rRNA processing through protection of the mature 5'-end of the 15S rRNA and stabilization of mS47. The removal of the 5' precursor together with the dissociation of Ccm1 may be catalyzed by the 5'-3' exoribonuclease Pet127. Involved in the specific removal of group I introns in mitochondrial encoded transcripts.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Binds to mitochondrial small subunit 15S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040212.1">
        <property type="nucleotide sequence ID" value="XM_019183199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6D6"/>
    <dbReference type="GeneID" id="30200445"/>
    <dbReference type="OrthoDB" id="3981214at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936:SF1">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN GUN1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936">
        <property type="entry name" value="PPR_LONG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="603" mass="70858" checksum="9D0B105E6258A6EC" modified="2017-01-18" version="1">MAELEIRSKLPIILELYNHFPGKSFEKGRIIKILMRVFSQLEEYSLVEIVFSRYLELPELKVRYCNMGLKAVIFNGNQELAKQLLYQMVYSHLPMNDSTLKSFLSAVQKSGTFQSVRFAVDLLEKNPNVPVDNQSYSTIMKCYAKNATESEFKSLANVLEKRGCDLYELELHKFYHDLIQQVKIDDEKLWSKILEIRSNLSKKSYLLNSFYLEMMSIFSYKLRRNDMFRLVELLSEDSIEFSDELNNILFSFFAKVGDVDGLLVYFSSFKDKKIHIQPQFIYSLWRTLITKEPELAKIVTDTIEEFMHKHYSTAQLENKFLKAISLRHKMRNSLPDYVPAVSDKRLARSLEVLNDLLHQKKDAEIIVLINDRLRQGLRPEFPLLMSAMVGLIKIKSPEILILYKLGCQLYTDMIVDFDLVWLKKTIYDLRAEHNNGLKLFDTGSALIGDFKLKHGDKMTHSHFNDLANICIDIFNYDLAIELLQESRASSAEVEKRNNLNIYITLLKALARKKDLPNYLHVMKLFYTDDDVKLSTFGIKRLKTGRQYFIKKNAEEPFENFEEFLNQFDIHHEKIKEKYTTQREEAEESCRNAIEFFEKWNKET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6E1</accession>
    <name>A0A1E3P6E1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_62976</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039624.1">
        <property type="nucleotide sequence ID" value="XM_019183468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6E1"/>
    <dbReference type="STRING" id="683960.A0A1E3P6E1"/>
    <dbReference type="GeneID" id="30200714"/>
    <dbReference type="OrthoDB" id="44467at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071913">
        <property type="term" value="F:citrate secondary active transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006843">
        <property type="term" value="P:mitochondrial citrate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049563">
        <property type="entry name" value="TXTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788:SF5">
        <property type="entry name" value="AFR253WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788">
        <property type="entry name" value="SUCCINATE/FUMARATE MITOCHONDRIAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="27"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="185"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="269"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="160"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="160"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="179"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ60417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="368" mass="41212" checksum="D5DE853526D32AD7" modified="2017-01-18" version="1">MSDNLPPSSKHSRPSHSRLSRDLVVEPNLLTAIISGVAASIAQTTMTYPFEFLKTTTQLHRNLPGAKQVELFHQFKHYFSGCGALNIGNALKSGSRFILFNSTSKFMSTENGKTSAPRLVIAGAMTGFVESLWIIPFENIKTTMIENSLHYSAIENKEKESNLKKKNEKVNYKPPTPKKQVQTSPKTFHKPTSSLPKDAHPFLLAKEKWDKLPSSSFITTIQEIYQTRGFRGFLQGTVPTLIRQCTNSAVRFGTYTSLRQLFQPHGGELNTYLAFAIGVTSSLAVVGVTQPIDVIKTRMQSREAYYTYKNSLNCAYRIFVEEGLKSFWSGWTYRLLKVATSGGVAFAVYQNVENQITRALKEQPFQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6N3</accession>
    <name>A0A1E3P6N3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_28062</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the complex I NDUFS4 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039744.1">
        <property type="nucleotide sequence ID" value="XM_019181445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6N3"/>
    <dbReference type="STRING" id="683960.A0A1E3P6N3"/>
    <dbReference type="GeneID" id="30198691"/>
    <dbReference type="OrthoDB" id="3089at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.190:FF:000001">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 21 kDa subunit mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.190">
        <property type="entry name" value="atu1810 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006885">
        <property type="entry name" value="NADH_UbQ_FeS_4_mit-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038532">
        <property type="entry name" value="NDUFS4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12219:SF8">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12219">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04800">
        <property type="entry name" value="NDUS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5 9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ60537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="139" mass="16404" checksum="654A290FCE79CCAC" modified="2017-01-18" version="1">MQKNESEVIEKQEDHGELPKEIVSGAPQELVTERVVRIYKESKPATQSGIWGRHNWKLDWDVLGKGHRWENDLIGYQSSSDYMQGTIMKFDTKESAIKFAQGQGWTFFVQEPNERRFKKKDYSKNFYHSPTKLKHIRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6N8</accession>
    <name>A0A1E3P6N8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_89793</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039829.1">
        <property type="nucleotide sequence ID" value="XM_019186192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6N8"/>
    <dbReference type="STRING" id="683960.A0A1E3P6N8"/>
    <dbReference type="GeneID" id="30203438"/>
    <dbReference type="OrthoDB" id="2392202at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207:SF0">
        <property type="entry name" value="RIBONUCLEASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="RIBONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="3">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="216" mass="24219" checksum="96BE0A493FCCAC90" modified="2017-01-18" version="1" fragment="single">IITNDRILKTVFTHKTAATDPALYDVGDCYERLEFIGDAFLNFVIALILFQKFEKAPEGGMSWYRDRLRSNLVFSGWAADYGLAKHLLPENMAKTIGEDSSKRAADIFEAYVGGLLISQTSSASNNGSFFNFAGCKPIEDWIKSLVDSKILLLQNGEEIHVRPNQKTHRSSSSSSSSSSNLQNLECWGPVYDEKYKYATEQLYSMLNPNFAPGYEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6T7</accession>
    <name>A0A1E3P6T7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_61238</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039879.1">
        <property type="nucleotide sequence ID" value="XM_019183137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6T7"/>
    <dbReference type="STRING" id="683960.A0A1E3P6T7"/>
    <dbReference type="GeneID" id="30200383"/>
    <dbReference type="OrthoDB" id="3981245at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051767">
        <property type="entry name" value="Nucleoporin_NUP42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46527:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46527">
        <property type="entry name" value="NUCLEOPORIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18345">
        <property type="entry name" value="zf_CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="185"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="281"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="189"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="206"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="216"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="281"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="348"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="379"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="404"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="488"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="507"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="526"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ60672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="566" mass="58053" checksum="846F266760E698B6" modified="2017-01-18" version="1">MSSKPPCKFYAQGTCRKGSSCPFPHVNPNNNRNNYNGSGRSTFGQVDINKRLRDFWDQSTLDRKSTEISEELRDFETFESLSSSAFGLADPAVENLISGRDYSFEESRLDFYQAQQANAAQDYEKMINIRTQDMKNCVDFLKKDTRKPARYTQVTTREKFEHPATYAAKPFINFPLDLTGNSLSNNTQSPFGSSPFGGSSNTTSNPFGAQTSNPFGTSSTGTGAFGSQTSKPSIFGSTSLSQNPSSSPFGQSSLGNSSSTGGGAFGSQSLGGASAFGQSGFGSSAASTSPFGTANPLGSTSSATPGTSAFGSAGFGSTSSATNNNAGSAFGGSGFGSTTGPSFGGASLGSSNTKSAFGSSGFGQPSTTSTSPFGQQKPAATSAFGTSGFGTSNSTSSPFGSAGISTNKDQQNPFGANSTTGDKASTSSAFGQGSTSQSPFGQVEQKTASPFGQSSTVSPFGQSAFGQSTTQPSSSSAFGQSGAIGQSPFGQSQNTAGLGSVSFGNAFGNSLQQPSGQSFRPTFKQSAKEEARSKEDELGDEVKAQFTASAFTIGLVPEVPPPLAYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P775</accession>
    <name>A0A1E3P775_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_77914</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040482.1">
        <property type="nucleotide sequence ID" value="XM_019184632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P775"/>
    <dbReference type="STRING" id="683960.A0A1E3P775"/>
    <dbReference type="GeneID" id="30201878"/>
    <dbReference type="OrthoDB" id="28092at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026895">
        <property type="entry name" value="EMC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011678">
        <property type="entry name" value="EMC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573:SF0">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07774">
        <property type="entry name" value="EMC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133717" description="ER membrane protein complex subunit 1" evidence="12">
        <location>
            <begin position="18"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="854"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="domain" description="ER membrane protein complex subunit 1 C-terminal" evidence="13">
        <location>
            <begin position="670"/>
            <end position="881"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ61275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="885" mass="101115" checksum="AA74317B2699B2F1" modified="2017-01-18" version="1">MKFQFLGLICLITTVLAVFSDEAYVTDWKIRQIGPLDSSNILYPRKEILNLLTKTNTLAGIQDGNVRWRVHFDDVVEDSKQSVIQLGDNIVTGLNYLNRSLLQVWDPIDGFWIKDLAIFTSKEISAIKAHGENVIVVFKNGEVVLVNNDGDIKSFESISKFDNVIISPLENGNFIILFNEQDTENTHYGFYDGEKFHTHTLQISWSSIKEFHGAKLVYQTIKYYVLQFDKNTGVVSSPIPIQGKFDEFIAIRIDSLIAFERNHNIEILKLDGSLLFTIPNVHKYLLKFVENSFILFTEYHVNIIDPDTGDEISSFFLGDEKLPFDLIKSISVSTDGVENIHSMFEFSNQTYSYVWNNVKVWQRDASLSQVVAHAIVDLEVETSLTPDDLLHEEELNVFQAYLFRLTKHAKELKQLYESFWETLPLIATGEVSLFGQTKENNEYFGFIKYLILGTKSGRVIALNTLNGEKVWSFDTQQDDILSIETVENERLYVFTKSGPRLELDAKTGEFIEKKLIVPPLKVEKLDSDDKFYVEIENEFAVILSDDPSNLNSSSSSFIIKHDSNKIQGLKIEGKKVLQTWKFETTADEEIVAFASKDEDEKIANVGYVLGDRSVLYKYLYPNFAAFAVLNKPTKTLYIHIIDSITGEIVHTAYHDEHVHEDEHINILFGEHWVIYSYWSNKPYPEQKIVVIDLFESLKSNEKFSNNTSSSLDYKHPPAVLSKSFILPFKINSMAISKTRFGVTTKSIIISLTNGQILYLPKYILNSRRVQDRPLTNEEKSEFMLTPYDPIITIDDNFVISHHRQVMGADTLISVPTNLESTSIVCSYGLDVFCTRVSPSLQFDKLTSGFDKVKLIASILMLIIIVLILKPLKDSRNLKSLWVIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P789</accession>
    <name>A0A1E3P789_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_83369</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040357.1">
        <property type="nucleotide sequence ID" value="XM_019185531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P789"/>
    <dbReference type="STRING" id="683960.A0A1E3P789"/>
    <dbReference type="GeneID" id="30202777"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000134">
        <property type="entry name" value="MFS sugar transporter protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF24">
        <property type="entry name" value="HEXOSE TRANSPORTER PROTEIN (AFU_ORTHOLOGUE AFUA_8G04480)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="2"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="493"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="498"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ61150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="513" mass="57639" checksum="EC99B36382E5126D" modified="2017-01-18" version="1">MLMVIISISSANTGYDGSLLNAFQSIPDWMRAMSNPTGATLGALSNGVVFGVALGFFVTSYVSDRFGRRNTITIGNAFMFGGSVVQSCAGTWLTVSGERDHDDGMRTYAMFLIARIIIGFGNSMTMVASPSLISELSYPTHREVVTAFYNSNWYLGAIVSSWCSYGTRNLSSNWSWRVPTILQGFFPLIQFFLIYLVPESPRFLVSKGRNDEARDILLKHHGGGDESKGGALVDFELAEIEMAIEEEKLADKSSYLDFFKTPGNRKRLWILVWIAILMQLSGNGLVSYYLNKVLVSIGINNTDEQLLFTGGLMIYNYVISIFINLFAFQHTKRRPMFIISVSLMLLFYVIWTILSAINQQRNFEDKALGKGVLAMIFLYYFAYNLGLNGLPYLYMTEITSYQIRAKGININVFGQELGQIYNNFVNPIAMDAIEWKYYIVWCCILAVELIVVILTFVETSGRTLEEVAEVFDEKIENFDMKLIKEKAQISHAEHVDNSNDGSSVTDKSTGLMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7A4</accession>
    <name>A0A1E3P7A4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Vacuolar protein sorting-associated protein 29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_29479</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the VPS29 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040518.1">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="project" value="GOC"/>
    </dbReference>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="entry name" value="MPP_Vps29"/>
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        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
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        <property type="entry name" value="Metallo-depent_PP-like"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VACUOLAR SORTING PROTEIN VPS29"/>
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    </dbReference>
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        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005945"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ61311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="184" mass="20191" checksum="F75A39E90363AC9B" modified="2017-01-18" version="1">MLVLAIGDLHIPNRAIDLPAKFKKLLVPGKISQVLCLGNATSSPSTLEFLKNLSPDFQIVKGEFDENTSLPLSLIITHGSLRIGFTNGFTIVPRGDPLSLLTSARQMNVDVLIWGGTHKVEAYTLEGKFFINPGSATDDTDDIYDSVPSFTLLDIQGSVITLYIYTYIGGEIKVDKVSYRKDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7B6</accession>
    <name>A0A1E3P7B6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sodium/hydrogen exchanger</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_28608</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040523.1">
        <property type="nucleotide sequence ID" value="XM_019181514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7B6"/>
    <dbReference type="STRING" id="683960.A0A1E3P7B6"/>
    <dbReference type="GeneID" id="30198760"/>
    <dbReference type="OrthoDB" id="196264at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004709">
        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00840">
        <property type="entry name" value="b_cpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF187">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0050">Antiport</keyword>
    <keyword evidence="6 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0915">Sodium</keyword>
    <keyword evidence="8 9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="242"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="364"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
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        <location>
            <begin position="63"/>
            <end position="460"/>
        </location>
    </feature>
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            <begin position="503"/>
            <end position="531"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ61316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="599" mass="66630" checksum="2F1696A1C9B3AE20" modified="2017-01-18" version="1">MSGLILGVGKYLIKRAVADDGDVPDVTIPDDGEGDTNPVTEEIFSSWALFILLLLLISALWSSYYLQQKRIQAVHETVLSIFYGMIVGLIIRVSPGHYIQDTVTFNSSYFFNVLLPPIILNSGYELHQANFFRNIGSILTFAIPGTFISAVVLGVILYVWTALGLESVNISFVDALSVGATLSATDPVTILSIFNAYKVDPKLYTIIFGESLLNDAISIVMFETCQQFHGRDAKFTSFFEGIGLFLMTFTISLLIGVIVGILVALILKHSHIRRYPQIESCFVLLIAYESYFFSNGCHMSGIVSLLFCGITLKHYAYYNMSRRTQISTKYIFQLLARLSENFIFVYLGLALFTEVELVYKPMLIIVTTISICLARWAAVFPLSRLINWVHRMRSNGQSLPDEIPYQYQMMTFWAGLRGAVGVALAMGLQGEFKSTLLATVLVVVVLTVILFGGTTAGMLEVLNIKTGCVDEDESDDEFDIEAPKAITTNSVLANRRFNKSTPAVPYSDIRNRSSPNINPQEDLLRDDDAPTDLDEIPPLMPASVNPMERSSTSIVGTVQSILHTDDRARWFQNFDEQVLKPVLLDVDPSKNNRNGSPPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7C0</accession>
    <name>A0A1E3P7C0_WICAA</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">WICANDRAFT_77953</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ61319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040526.1">
        <property type="nucleotide sequence ID" value="XM_019184640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7C0"/>
    <dbReference type="GeneID" id="30201886"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="250"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="388"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="436" mass="49095" checksum="A6F61DCC5E497E4A" modified="2017-01-18" version="1">MSPNNQSEQHPPESVVYRFKVNEQLPNYSQPQGAASTDSPSPVLYATPEASSNHAQANGTPTLDGPQASVTPFEAHSAARDPSASSGSNQGATFKYISSNELSERSSTSSAHKPKSRIAADSSKPSHSLSLEEAKAMFETADKTTRFQHPTDPKEKKREDILLMKSILNYKPFSDGVNKYETLRDVTIDLIRSYHRRLSMKKVRSYTRKLMDELTIRQENSKNIPDGIDVLSDLEREFFDYLKERNYHQKSIDSTSGTNQLKEKTTNHKNTTFQDPHMQEHLQLMARQAVHKRAAEDFIEHERHFNGPPIPPPPPPPGIPQIIPSDMAPKRIRMVEPTSDDVLRGIAESLRALNETIIMNSNTNTNLIQKVTEALLTRDGSRRDENTRTESVNESESINKRLEKLEDNIDNIRNDLSGISSSLSMMNDFIKSKLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7J2</accession>
    <name>A0A1E3P7J2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Chaps-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_61885</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
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            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7J2"/>
    <dbReference type="STRING" id="683960.A0A1E3P7J2"/>
    <dbReference type="GeneID" id="30200511"/>
    <dbReference type="OrthoDB" id="434695at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034044">
        <property type="term" value="C:exomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015374">
        <property type="entry name" value="ChAPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975:SF1">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09295">
        <property type="entry name" value="ChAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="512"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
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        <location>
            <begin position="443"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ61328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="677" mass="76589" checksum="7FA0329C5CFD5701" modified="2017-01-18" version="1">MITSQGSIPEVKEDIPGSSIDERSNRIRTFQDLGPPDIVSLTKHVPGSKSPEVGTYLYYTGLDNSSSSSVAAHLNSLANIIGSTPQTWFGKSRPWKVTKAVYASYNAFSKVDVVVAVHIPGAVSSYVIDGYRERIQETDRIWLEVFTSGVIRSLLTSEEDDEQVNSVVESRNLNPFPTVQLAKQFLDAFEELFEEGPNIGSAPEIQTPTLISNYLVDAFLKAVELTGLYDYALDILNRLREKDEIVVSLIVKVLFLKDEEIKAVQVMYEGIKKNPRDAALLSIQSQFLIEKNHLDLALTCAIQAVNSAPSEFNPWANLAKVYLELGNVEQALLALNSCPMAAYKEKFHLKRIIPTTPEKLHLPLPIDVTLDEVSSLDSARVAQEHNSVDQALLNLQAANLKSTFARAYDILTEIVHRTGWESLLKYRAKIFVMEEEYRSKSSSTANIIDPKTNGNSTEASSEDENSFKKKRLCERWLDNLFMLLYEDLRTYTMWQAEMIHFQAQKTQYEKLPVEWELLGMVAFRLHHYKEAAAAFQRALELRFASTSTRNLLKYYQLEIKALKQKGERSQQVSNGHNNISTTSIDHAHLSPTQVSKKITSLDESILDAVVKLTVWNHRWYCEFAPYLLTALSKVVGREGLVKIQNEVRANYPDTGVYELLDESFEFFKQFQIFGAET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7K1</accession>
    <name>A0A1E3P7K1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_90912</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040618.1">
        <property type="nucleotide sequence ID" value="XM_019186265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7K1"/>
    <dbReference type="GeneID" id="30203511"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="60" mass="6837" checksum="01A98973DEDD61F7" modified="2017-01-18" version="1" fragment="single">MEFPWIYQLMGNLLWSECISNSLILINYIVGLWAKHIQKTSSSSSIPVAKHQANNYQVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7V0</accession>
    <name>A0A1E3P7V0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">STB6-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_90825</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040597.1">
        <property type="nucleotide sequence ID" value="XM_019186263.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7V0"/>
    <dbReference type="STRING" id="683960.A0A1E3P7V0"/>
    <dbReference type="GeneID" id="30203509"/>
    <dbReference type="OrthoDB" id="19806at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070822">
        <property type="term" value="C:Sin3-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038919">
        <property type="entry name" value="STB2/STB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059025">
        <property type="entry name" value="STB6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31011">
        <property type="entry name" value="PROTEIN STB2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31011:SF2">
        <property type="entry name" value="PROTEIN STB2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25995">
        <property type="entry name" value="STB6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STB6-like N-terminal" evidence="2">
        <location>
            <begin position="44"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="733"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="733"/>
            <end position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="766" mass="88323" checksum="24BAE87AC0AC281D" modified="2017-01-18" version="1">MAPSVSQAETLQAPPSSHFPGWGSSLNLQELQIQGASNDNQEVTYLFPDTIALESICDNQESQYEITSIRGFEIYIVEQWACERKYGTVITAFTGDSEHEIKVGKVNLPLDTSKWSDKLMHFFQEIAESGAKPKKTEEGFLFITNLSSFPSNLNLLPIPCGCVLDTWEYFKVNVNLRRMHCGGRSALLLCSPSDASGDKFRQIYKTHPKVDVMYAVKELVMLVQICLVDFKLLDPNFVDGLLCDKTVEKIKDWWRLFGSAYYGTTPKDGLLGPTTVAAILGFVLSCHFRLDIAGSDFPKEPFNYFSFRVNVGKFQKQYNLKRTWYLDPITVEKLFRVTTKISNTDISKLKKVVKSTVQDISGKGSSIQYAPEVLTLDLEKLIKYFHCSARLTYLWYGKGDIRDIKGFDYHERGIPASSLRSGVGKIRSLPRRLQDDNVSEKRAKERQSETCKEVEDEPQEQQLCQQLDERFKKQIMMKKKLTTDESFNNELRRRCSFPFIRNEISVGQIEYENLPISRVSNITTNTIMKRSKSFSFVENSLSRWDFVPSPYNFGDKVKSLKSKLYIHQHSTEEFNKVKNQYAKTVEKSHNELAKLNQMVKPINRQYEIQTQAGERAKMKMEDIQALSARLEYELRILSTRVRDTEDSVDQFVSKVQSLDKSISESNACLKFKSKDWDGHSENIDENNMMEICWYSIVSRFDVDRLKHLWMKFDPSFGYANWIRDKLFCSNEQQEQPLPKTTVSSYESKKQADNKNSFVNTEKAKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P836</accession>
    <name>A0A1E3P836_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_104168</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040244.1">
        <property type="nucleotide sequence ID" value="XM_019180402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P836"/>
    <dbReference type="GeneID" id="30197648"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="814" mass="94996" checksum="A8B126DE42C9D1C2" modified="2017-01-18" version="1">MNSKVTLGSFSPYNRISGYMRRALPGKLPRYVQILKQANAKSDSQEDQSKSRKDVVVEVPGVVQNWNWKNFKDLVLLDMKYKDAKFLFKPVDGLRVPKVNSRVALDRLLNNQISEHYVSDEASKNDDYIKRFPAEDTQIYYLQTVIGESSGTEVEKPKDTQVPFESAIGPLKFNISSIVIFQNTKALGITENHPNFQELIETKVNLPIIGEVPFIKVDKEQLELEDDEIITAITPSYVLPHQVYFPNARPAEVNKFRNFITNDKVDAHTLGALIAREFGLENVELYTVPYIVSKHFAKKRTPLTGVHQGKERFLPYYETHEIIAQGPLGKPLDNPNLWFTNITQAGWRRVEHQRSGTAQYNIENLTYIQLAKLLHEGKTLNKMNNKKEQLMHYDKFSTDMFRGLILSQKDISDVEIDAEFQVIDLLSNVLNYHLITNKSRLNDGIDGFHPRVSDIYEGDKALLLKPWDRVIIDRFYKIENKKDVDDFIKNFAIYQMMVHLEGDSDKNRWKFVFEITSNLLIRFLNDHGKAYYPSISNYFLKAFGYTKEYKTNILPGETTNSWTHYIEYPIESVGNTALNDELFSVAEHIFSVVRIGPFYKTDKVILTDNKIILENKALLESINPEGELIFNELDKDFKLNYFIDKICEIQPQDKRNDRQEPRPKSNTQIIFYRNYFRGDVLPEFIFKKLNYLKFRVSPEIKTDYLRKDLLVKLLTDLKRNLSTREADQVNYPLTRIERDRLYSFLKFFVDNLPEKFFSHPLILRITPDKFNKTDTDAKYASMSNRISGALENYERSEARLDDIIMDFLQRHNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P851</accession>
    <name>A0A1E3P851_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_90345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040329.1">
        <property type="nucleotide sequence ID" value="XM_019186233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P851"/>
    <dbReference type="GeneID" id="30203479"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ61122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="138" mass="15754" checksum="A842CA4D7745AFEA" modified="2017-01-18" version="1" fragment="single">PLNEIQDFKNIKSLVILGDNDIAFGTNLKVEHLSLVLPKDKLNVQGLKRLDLAIDPRLIDNMNALEFFKYCPIQELNVTIRDLTTILKYKPFLLFILSLKSLNKLSLRCSNQPQPSSLHISTSDLYDTTAQFFQSSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P896</accession>
    <name>A0A1E3P896_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuolar protein sorting-associated protein IST1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_78152</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IST1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040737.1">
        <property type="nucleotide sequence ID" value="XM_019184672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P896"/>
    <dbReference type="STRING" id="683960.A0A1E3P896"/>
    <dbReference type="GeneID" id="30201918"/>
    <dbReference type="OrthoDB" id="29853at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042030">
        <property type="term" value="F:ATPase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099638">
        <property type="term" value="P:endosome to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.60:FF:000002">
        <property type="entry name" value="Vacuolar protein sorting-associated protein IST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.60">
        <property type="entry name" value="Vacuolar protein sorting-associated protein Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005061">
        <property type="entry name" value="Ist1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042277">
        <property type="entry name" value="IST1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161">
        <property type="entry name" value="IST1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161:SF5">
        <property type="entry name" value="IST1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03398">
        <property type="entry name" value="Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="179"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="33627" checksum="8EB2B536357F1EEA" modified="2017-01-18" version="1">MAPVISSQGRLKVTLKMAISKLRFTQTKRLALVKQQRRQMAELLSLGKEESAKIRVENIVREDIYVELLEMLELYCELLLARIGLLDKKECDPGLEEAVKTIIYSASHTDLKEMHTVRDLLIHKFGAEFARAAIENDDNVIPEKITKRTAVEAPSQELVSLYLKEIAKAYDVPFSELEAEEAADDEDDDDDDDESNGGEGKPIQLEEPIEDYPEERISTPRKLSAASLPNPNEAEKSPISVRPPHKSSDNPHPTVKIPDELQKNVSKKKRTSDAGLKNSSKEKDDLDALRKRFEALKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8X0</accession>
    <name>A0A1E3P8X0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Pentafunctional AROM polypeptide</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="17">3-dehydroquinate synthase</fullName>
                <shortName evidence="17">DHQS</shortName>
                <ecNumber evidence="17">4.2.3.4</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="17">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
                <ecNumber evidence="17">2.5.1.19</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="17">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
                <shortName evidence="17">EPSP synthase</shortName>
                <shortName evidence="17">EPSPS</shortName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="17">Shikimate kinase</fullName>
                <shortName evidence="17">SK</shortName>
                <ecNumber evidence="17">2.7.1.71</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="17">3-dehydroquinate dehydratase</fullName>
                <shortName evidence="17">3-dehydroquinase</shortName>
                <ecNumber evidence="17">4.2.1.10</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="17">Shikimate dehydrogenase</fullName>
                <ecNumber evidence="17">1.1.1.25</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="24" type="ORF">WICANDRAFT_86575</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17 18">The AROM polypeptide catalyzes 5 consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:21257"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate = 3-dehydroquinate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21968"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="EC" id="4.2.3.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>shikimate + ATP = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>shikimate + NADP(+) = 3-dehydroshikimate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17 18">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="17 18">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="17 18">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="17 18">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="17 18">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="17 18">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 17 18">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17 18">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17 18">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17 18">In the 2nd section; belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17 18">In the 3rd section; belongs to the shikimate kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17 18">In the 4th section; belongs to the type-I 3-dehydroquinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17 18">In the C-terminal section; belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">In the N-terminal section; belongs to the dehydroquinate synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">In the N-terminal section; belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.4" evidence="17"/>
    <dbReference type="EC" id="2.5.1.19" evidence="17"/>
    <dbReference type="EC" id="2.7.1.71" evidence="17"/>
    <dbReference type="EC" id="4.2.1.10" evidence="17"/>
    <dbReference type="EC" id="1.1.1.25" evidence="17"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040871.1">
        <property type="nucleotide sequence ID" value="XM_019186053.1"/>
    </dbReference>
    <dbReference type="STRING" id="683960.A0A1E3P8X0"/>
    <dbReference type="GeneID" id="30203299"/>
    <dbReference type="OrthoDB" id="197068at2759"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00085"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003856">
        <property type="term" value="F:3-dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00502">
        <property type="entry name" value="DHQase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08195">
        <property type="entry name" value="DHQS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01065">
        <property type="entry name" value="NAD_bind_Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00464">
        <property type="entry name" value="SK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1090.10:FF:000007">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1970:FF:000007">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001256">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.65.10.10:FF:000007">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.65.10.10:FF:000008">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="entry name" value="Enolpyruvate transferase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03143">
        <property type="entry name" value="Pentafunct_AroM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00109">
        <property type="entry name" value="Shikimate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016037">
        <property type="entry name" value="DHQ_synth_AroB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030960">
        <property type="entry name" value="DHQS/DOIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056179">
        <property type="entry name" value="DHQS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001381">
        <property type="entry name" value="DHquinase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008289">
        <property type="entry name" value="Pentafunct_AroM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041121">
        <property type="entry name" value="SDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010110">
        <property type="entry name" value="Shikimate_DH_AroM-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000623">
        <property type="entry name" value="Shikimate_kinase/TSH1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01356">
        <property type="entry name" value="aroA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01357">
        <property type="entry name" value="aroB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01809">
        <property type="entry name" value="Shik-DH-AROM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090">
        <property type="entry name" value="AROM/DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090:SF5">
        <property type="entry name" value="PENTAFUNCTIONAL AROM POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01761">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24621">
        <property type="entry name" value="DHQS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01487">
        <property type="entry name" value="DHquinase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18317">
        <property type="entry name" value="SDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
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    <dbReference type="PIRSF" id="PIRSF000514">
        <property type="entry name" value="Pentafunct_AroM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01100">
        <property type="entry name" value="SHIKIMTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00104">
        <property type="entry name" value="EPSP_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00885">
        <property type="entry name" value="EPSP_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 17 18" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="13 17 18" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 17 18" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 17 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="14 17 18" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 17 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15 17" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11 17" id="KW-0521">NADP</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 17 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 17 18" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="3-dehydroquinate synthase N-terminal" evidence="20">
        <location>
            <begin position="75"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="3-dehydroquinate synthase C-terminal" evidence="23">
        <location>
            <begin position="189"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Enolpyruvate transferase" evidence="19">
        <location>
            <begin position="402"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="domain" description="Shikimate dehydrogenase substrate binding N-terminal" evidence="21">
        <location>
            <begin position="1257"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="domain" description="SDH C-terminal" evidence="22">
        <location>
            <begin position="1505"/>
            <end position="1534"/>
        </location>
    </feature>
    <feature type="region of interest" description="3-dehydroquinate synthase" evidence="17">
        <location>
            <begin position="1"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate dehydrogenase" evidence="17">
        <location>
            <begin position="1252"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for 3-dehydroquinate synthase activity" evidence="17">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for 3-dehydroquinate synthase activity" evidence="17">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <feature type="active site" description="For EPSP synthase activity" evidence="17">
        <location>
            <position position="821"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; for 3-dehydroquinate dehydratase activity" evidence="17">
        <location>
            <position position="1183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="46"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="81"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="112"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
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        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
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        <location>
            <begin position="137"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
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        <location>
            <position position="144"/>
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        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
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        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
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        <location>
            <position position="159"/>
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        <ligand>
            <name>NAD(+)</name>
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        </ligand>
    </feature>
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        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
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        <location>
            <begin position="177"/>
            <end position="180"/>
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        <ligand>
            <name>NAD(+)</name>
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        </ligand>
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        <location>
            <position position="188"/>
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        <ligand>
            <name>NAD(+)</name>
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            <begin position="192"/>
            <end position="195"/>
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        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
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            <begin position="262"/>
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        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
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            <position position="269"/>
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            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
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            <name>Zn(2+)</name>
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            <note>catalytic</note>
        </ligand>
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            <position position="285"/>
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        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
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        </ligand>
    </feature>
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        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
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        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
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        <location>
            <begin position="864"/>
            <end position="871"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044633"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR000514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF01761"/>
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    </evidence>
    <evidence type="ECO:0000259" key="21">
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            <dbReference type="Pfam" id="PF08501"/>
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    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF18317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF24621"/>
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    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="ODQ61664.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1536" mass="169082" checksum="A441F209A7B334AB" modified="2017-01-18" version="1">MASSGHVEKVRILNKDTIHIGYDLQDHIIDQLITTEKSSTYVIITDSNIVKKGYLDSYLTKFQSKLNNDQRVLSYVVSPGEANKTRETKAKIEDYLLSKGCTRDTVILAIGGGVIGDMIGFVAATFMRGVRVVQVPTTLLAMVDSSIGGKTAVDTPLGKNFIGSFWQPQYVFVDLTFLSTLPEREFINGMAEVIKTAAIWNEDEFTRLEQNASKFLATIKKRNSAGDTDLTPIKEHVFKLVSESIKVKAEVVSADEREGGLRNLLNFGHSIGHAYEAILTPQALHGECVAIGSVKEAELSRYLGILSPVAVARLAKIFTAYGLPISVDDKNFRKIVKSKKTPVDTLLKKMAIDKKNDGSKKKVVLLKAIGECYEKSASFVNDEDLRFVLTDETKVFPFQNDSTFNATIIPPGSKSISNRALVLAALSEGECKITNLLHSDDTEHMLNAIAKLKGAEISWKDNGETLIIKGNGGDLKSTDQELYLGNAGTASRFLTSVATLVKSHQGQNHVVLTGNKRMTERPIGPLVDALRSNGAKIDYLKNEGSLPLKISTTPLKGGHIELAATISSQYVSSLLMAAPYAETPVTLKLIGGKPISILYIDMTIELMKKFGIEVEKLPNYTYAIPKGSYVAPSEFVIESDASSSTYPLAFAAMTGSTVTVPNIGSSSLQGDARFARDVLKPMGCKVVQTETSTTVTGPPRGSLKPLPEVDMEPMTDAFLTASVLAAIATDGGENRTNIIGIANQRVKECDRIAAMVHQLNKFGVVANELPDGISIQGVKISDLKTPEEGIFSYDDHRVAMSFSLLAGMVNNGPVTIEERHCTGKTWPGWWDVLHSSLQAKLDGFEPAQVETKQQINNKSIIVIGMRAAGKSTLSKWISSSLGYKIIDLDTVFEAQYGDIREFIKKNSWEEFRSLEIKVYESIISKHSHNTVISTGGGIVESNVARAHLSNYAKEGGIVLHIERNLDNTVQFLSEDATRPRYVEEIEQVWKIREPLYNEVSNFHFFAPFCNSNKEFLELRKNFEHYIFKIIGHQNINFEEFKRSFAYVLDIEDVEDEAEHLEVNSYGANAIELVLTKPYSKRFIEQSISILRKYTDLTSILTLTSTKDLAKYEEIVNLAFKNGIDFVTLDLTLEAELLRRLINNSGYTKIIGSYVDTTGELKWGNDVFLGYYKFAQVLNADLIKLVGTATSIVDNFELENFRAKLADSNLISYNLSDFGVLSRVTNPLLTPISKSESIKQLNSLYYKIGGGLTKKYYVVGTPISHSKSPILHKTGFEILGLPHSFEKFETDDVKKVGELLKDPQFGGAAVTIPLKLDIMEYLDELTESATLIGAVNTVIPLGSGKFKGTNTDWIGIKDSLLNQGFPNVSADSEINGLVVGAGGTSRAAIYALSQLGCKTIYMINRTSSKLQLIKDHFPEDFNIHILDSIDDVENSKPISVAISCVPSDLPMDPTLLNKLERLLNKGAANKGKAGFTPMLLDCAYKPRVTPVMELAKAKFEWNIVSGEEMLVNQGVAQFKIWNSTVPPYKAISDAVNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P915</accession>
    <name>A0A1E3P915_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_76080</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041098.1">
        <property type="nucleotide sequence ID" value="XM_019184319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P915"/>
    <dbReference type="STRING" id="683960.A0A1E3P915"/>
    <dbReference type="GeneID" id="30201565"/>
    <dbReference type="OrthoDB" id="3980677at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF46">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="31"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ61891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="602" mass="67998" checksum="A051B59FBC0F823F" modified="2017-01-18" version="1">MVKHPYEQVIKFEGLGTLKPNRARTKRSRNGCFNCRKRKKKCDEMKPSCSSCINKCISCVWPKDGGDKLPKGYRVPSSTSSEMNPNEGTIFIPVEFDSKTPEVEERSPVSSIDFFESPKGMTMIPYSNVAELIDDFGSPDSTSYSLDLENSSTPSYKITFLSPFSSDPDQRFILACTNGFIESVGPQYTHPLLTTTATFSPFVNNNPISQKVAIACGCAFLAWQNPELTGLSTQKYYTAFNALETYVNNNESAYKESWVGAAFQLMCLCSKITWSCSSDNCIANLVNSYKIIRRRFIETTRYGKKIQKSDKISSLEPQSDLAHYLKDFKNDALQMEIENSLIDLDDFSTEVFESHFEKMFVESFLFNYSIALLVSEQFYLLPNPFEVFRDLRQFLKTPLFSCDVAWMNNPVFGASLDAYELAAKASYLLRNLNDPNMLVTAKKLYDLASFYPSPMIPSEIRVNSTKYKNLRDSVLMAEIVAKVSKILLRKLIIPEITEMDSVVQKDLSSIFIKLKSISSDSRVKIISCWPLLIAGAATQNEEQRELLTKTLLEAGALTHGRHVYTVLDALDIAWGKSPECEEIKTRGLDILFDRRVVGKVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P964</accession>
    <name>A0A1E3P964_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">V-type proton ATPase proteolipid subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_76040</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Proton-conducting pore forming of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Proton-conducting pore forming subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11 12">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1). The decameric c-ring forms the proton-conducting pore, and is composed of eight proteolipid subunits c, one subunit c' and one subunit c''.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Vacuole membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041057.1">
        <property type="nucleotide sequence ID" value="XM_019184311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P964"/>
    <dbReference type="STRING" id="683960.A0A1E3P964"/>
    <dbReference type="GeneID" id="30201557"/>
    <dbReference type="OrthoDB" id="1744869at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18175">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18176">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000003">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011555">
        <property type="entry name" value="ATPase_proteolipid_su_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01100">
        <property type="entry name" value="V_ATP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <keyword evidence="4 12" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="13">
        <location>
            <begin position="19"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="13">
        <location>
            <begin position="96"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ61850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="161" mass="16563" checksum="B2EF728414A05A4F" modified="2017-01-18" version="1">MYVAICTQLLRKITNSSTGCAAAMILSCAGAAIGTAKSGIGIAGIGTFKPELIMKSLIPVVMSGILSVYGLVVSVLIAGGLSPTEEYTLFNGFMHLACGLAVGFACLASGYAIGIVGDEGVRKYMHQPRLFVGIVLILIFAEVLGLYGMIIALILNTKGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P987</accession>
    <name>A0A1E3P987_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HORMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_14964</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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        <source>
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    </evidence>
    <sequence length="448" mass="50820" checksum="D4E005E1D058C79C" modified="2017-01-18" version="1" fragment="single">TKTSITCEQSQKLIQTMLTMSFGCVAFLRGLFPDENFVDQRFVPNKFEQGYNPNDPKSKNDSIRVKTLARGKSTKADLFLDWIDKGATDAIRRGYLKSLSFAIFLNKDDPNDLHEVYTFFFDYKNDDIHFSINDEDEPISLLDSRKMLQQLMKRFIIITQSLEPLPEERNVSMRMLFNESCPKDYQPPFFKDASDEPSAILKAPKTSNVDRYSAGSVDTSFHSIQVKVLSRPILISNENELIEIDPFEASQFKTPNMIKNAQLPPKSLNRFVKREPSQTTNMLRSFLESSQPEVKPTQAINHVKCECGSTEESDVSGTIKCASCDGILHGSCYGSSSRRQETTCFTCRSKAMGGIKTHQNLRLLFIARKLYKLFSKVDIPPSTRIIHEQLGFAISENTKATASVISFFFKKCILVLEDEPRKLKKQVGDATFQKGSSYVDVDIDGIYV</sequence>
</entry>
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    <name>A0A1E3P9M2_WICAA</name>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name evidence="2" type="ORF">WICANDRAFT_82220</name>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
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        <lineage>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
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                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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    </dbReference>
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        </location>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ62113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="346" mass="40099" checksum="992F8491BB5969AF" modified="2017-01-18" version="1">MNFDITFSDDEEVYHHKTQGGVNSLAREIDSDRYMRAELGLDLSDSDDFSGSENDMDAMAELYEQLEISDNYDPVRTEHFDEKGSLKVETKNADGKKKQKGKPQNGANKQENNSDTAPTKRRRERKKKENGSKDESKLKKEESEQQVPNKETKTEHEGRSKSHQRRVRRRKAEQLRKNGENGNDQTKESQDAVNTSNNEKKFKGKNQGSNKPKESDATKRTELIEQQRSTKPTKTRGKAQSDRHEKSKSPETQEKSENRTKEKTTKDENNQEKEKTPRRTKKKEELNRDKKEKKAKSKDQNIDKSNSKAQTQEASQKSNMDQLQETIKKRMKELKEMKEQALPHIA</sequence>
</entry>
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    <name>A0A1E3P9T7_WICAA</name>
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            <fullName evidence="4">SH3 domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="5" type="ORF">WICANDRAFT_87398</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
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                <person name="Scheuner C."/>
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                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
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            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000142">
        <property type="term" value="C:cellular bud neck contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044697">
        <property type="term" value="C:HICS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990344">
        <property type="term" value="P:secondary cell septum biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052557">
        <property type="entry name" value="CAP/Cytokinesis_protein"/>
    </dbReference>
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        <property type="entry name" value="Ig_CYK3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46333">
        <property type="entry name" value="CYTOKINESIS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46333:SF2">
        <property type="entry name" value="CYTOKINESIS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24584">
        <property type="entry name" value="Ig_CYK3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="5"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="75"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="148"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="205"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="148"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
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            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="206"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="238"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="295"/>
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        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="884"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="884" mass="100243" checksum="CCCD9AD0CCFEAE66" modified="2017-01-18" version="1" fragment="single">MSVPQPPFRVRTIYSWSGVEEDDLGFIEGDIIEVDSLGDGNWWHGRLKRNKMTGSFPSNYVELIKTDLKNFSPSIRAKNSSQESSPIREVQASVRQTPTTSRIASPYSSPSAKASVLSQRASRHPYIASYPENEEVDDLQFSFQSSRNYYTPSQNTSNTYNPQSPYPTEQTHLSSQRSSQQHLPHSYSVPVRLEQQQGYVDKINPHTLQSPIHNSSSTTRLQMYASPPPPLPQHSVPVKSQSSQNLKSFDPFAPGNPYSKNDYSSRKSALLKQAAHKNMRDSQSTDFVAPYDPDSLNQSRSSTSQSSNVFSYSNGSYFTNSSNSHNSSYALSDFSATSAGSFARHRYEEQLKQKENLQSSGSGSHLLVEDRKLKKPSGIFKKMFSAKDVPPLPSVENLTSKVDGMNMEDKEINSWIELKVDLNRANSLGSKERQLREKRVRENEGYIIFEPHKQLSNINNNEVYQTEQKFDLHAFNLKHVDNYVRNLQIKQTVLSPEAFITNELGLKYTSKLEYLRALFIFCTEHFTIIDSAYSNEEYIPNVELIFKQRGADTYGMAFLFKCLADSMGLNCDIIVGSLKTPQSIVKHYWNAIVINGEWRFIDVSLGNLTNPVYEILANQPDEPNESFYFLTEPLDLIYTQIPDRFEDQHIVPPIDQMVALALPPCFPSFFKNGLKIQKFSNALTRLKDFEIFEIDLKIPKDIEINAVVQTKTSLTNTLAQVYWKHNERYCKIKGHLPENNPTGFINIYSGLKGTQKSIQNIHPLSMVIPVVHEGEYRPLDFVIRYPTVPAQLNDLYIKQPQNRNLSCRGEYVFSVSQYPSNGLASPPARTKIALQSPSGKITRFIKKDQSTPFGLWELPVKCNEIGTWRGLVSVDNGTSLCVFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9Y2</accession>
    <name>A0A1E3P9Y2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_87445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041432.1">
        <property type="nucleotide sequence ID" value="XM_019186113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9Y2"/>
    <dbReference type="STRING" id="683960.A0A1E3P9Y2"/>
    <dbReference type="GeneID" id="30203359"/>
    <dbReference type="OrthoDB" id="5563016at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="289"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="356" mass="40157" checksum="097BCBBEA89022B3" modified="2017-01-18" version="1" fragment="single">MFQYQQYNNNNNNNNHISAYEGQLRSMSSMSTMNSESDSTTFNHSTSSAQTSTNSSPYNYKAVQQGLQQDSIYEEQENSPDLMNEFYSSDNIDNKNHAQNFRQSMVYSDSFDSQQFQSVRNSIIVGNDAQVPTKLAMTNSPSFSALASILEKKTKFKPSMPSTIHEVHDDGQEDEFFGNPSVTHSTETLTVQKQQDSPNLIQLDDTETNIPREMSFDSILPSPLDPNVMIAPQYYERMNQQQQQNGIDNVDIFKTPEVPQLSSFNQVETPKSPPPKYESMLDVTEPEAISTSPITQSSTIEATPRRSETTEVESPKPLPKLRVNTNVLNSPQYKQRSSSLPILTNPPQQPQPKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA89</accession>
    <name>A0A1E3PA89_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NADH dehydrogenase [ubiquinone] iron-sulfur protein 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Complex I-15 kDa</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">NADH-ubiquinone oxidoreductase 15 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_24233</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Mammalian complex I is composed of 45 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the complex I NDUFS5 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041004.1">
        <property type="nucleotide sequence ID" value="XM_019181023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA89"/>
    <dbReference type="STRING" id="683960.A0A1E3PA89"/>
    <dbReference type="GeneID" id="30198269"/>
    <dbReference type="OrthoDB" id="9992197at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24141">
        <property type="entry name" value="NDUFS5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019342">
        <property type="entry name" value="NADH_UbQ_OxRdtase_FeS-su5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15224">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15224:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="14"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR619342-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ61797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="66" mass="7577" checksum="1142C0DBA3DEE6AE" modified="2017-01-18" version="1" fragment="single">MSSGYGFNGGSSRCFGEWQRFLECYTNADTSHPVQCTPQVDDYFECLHHKKEKARVMAIKSQLLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAE9</accession>
    <name>A0A1E3PAE9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Altered inheritance rate of mitochondria protein 29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_26184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0538 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041555.1">
        <property type="nucleotide sequence ID" value="XM_019181235.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAE9"/>
    <dbReference type="GeneID" id="30198481"/>
    <dbReference type="OrthoDB" id="937at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018794">
        <property type="entry name" value="UPF0538"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18444">
        <property type="entry name" value="UPF0538 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18444:SF9">
        <property type="entry name" value="UPF0538 PROTEIN C2ORF76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10209">
        <property type="entry name" value="DUF2340"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="16373" checksum="40BC67461F6FD95B" modified="2017-01-18" version="1">MADYEPLTSTVRPLSQSILTIRIIKSFPYRNVKNYILKDYDISVKTPKDLLNDMQKIISTEGGLRPYRTVKYDSLKIYTHAHGSKTMNLVINFEHDEDWFLDLESNKPLHELGVDNETEISLFNLEAYEEFKKNPEEKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBE0</accession>
    <name>A0A1E3PBE0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_88497</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041933.1">
        <property type="nucleotide sequence ID" value="XM_019186157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBE0"/>
    <dbReference type="STRING" id="683960.A0A1E3PBE0"/>
    <dbReference type="GeneID" id="30203403"/>
    <dbReference type="OrthoDB" id="47007at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009385">
        <property type="entry name" value="PRK12744.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48107">
        <property type="entry name" value="NADPH-DEPENDENT ALDEHYDE REDUCTASE-LIKE PROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48107:SF7">
        <property type="entry name" value="RE15974P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ62726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="254" mass="27873" checksum="C3D767FD9CA48C1D" modified="2017-01-18" version="1">MAPLDISLKGQTAIVTGGTKNLGAETVRELAKFGANLVIHYHSDSEKSNGESLVKEVKEKYGVDSILVQGNLAVEKDAKKLFEETKSKFGGADIAINNAGKVLKKPIGEISEADYDQMFDINSKASFFFLKYAYQYVNEKGRIIFLVTSLLAAYTPFYGLYQGAKAPVEYFVKAASKELHDKNISVNAVAPGPMDTPFLYGQETDESVAFFKTQALENRLTKVEDIVPIIRFLATEGKWLTGQTLYASGGFTAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBE8</accession>
    <name>A0A1E3PBE8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber evidence="4 12">5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">5-proFAR isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 12">Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_38303</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.16" evidence="4 12"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041414.1">
        <property type="nucleotide sequence ID" value="XM_019182535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBE8"/>
    <dbReference type="STRING" id="683960.A0A1E3PBE8"/>
    <dbReference type="GeneID" id="30199781"/>
    <dbReference type="OrthoDB" id="446074at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04723">
        <property type="entry name" value="HisA_HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000110">
        <property type="entry name" value="1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011858">
        <property type="entry name" value="His6/HISN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02129">
        <property type="entry name" value="hisA_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090:SF2">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ62207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="273" mass="30457" checksum="FC21D86209176F67" modified="2017-01-18" version="1">MTRFRGCIDIHSGQVKQIVGGTLHSDDNNNANANSQDHGQLSTNFVSTQPSSYYASLYKEHNVKGTHVIKLGSNKANDEAALEALRTWPGELQIGGGVNYENAQYWIENGASHVIITSWLFPQGEFSKERLVKLSELIGKERIVVDLSCKRKPIVEGDEVKWVVAINKWQTLTNFELNYENFKELSENCDEFLVHAADVEGLCKGIDEELVSKLSEWCDIPITYAGGAKNIDDLKTVERLSKGKVDLTFGSALDIFGGKLVKFQDCVEWNKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBI2</accession>
    <name>A0A1E3PBI2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_25079</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041990.1">
        <property type="nucleotide sequence ID" value="XM_019181097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBI2"/>
    <dbReference type="STRING" id="683960.A0A1E3PBI2"/>
    <dbReference type="GeneID" id="30198343"/>
    <dbReference type="OrthoDB" id="10266980at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071618">
        <property type="term" value="F:lysophosphatidylethanolamine acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036152">
        <property type="term" value="P:phosphatidylethanolamine acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050846">
        <property type="entry name" value="TLCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439:SF6">
        <property type="entry name" value="AAR085WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439">
        <property type="entry name" value="CT120 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="78"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ62783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="294" mass="33927" checksum="ADAC9EC12F572866" modified="2017-01-18" version="1">MASQIASFFTSNRYHDPLSHVELPFINQYVKPLYNNEVLDKHIHELVIIILGYHSIYVSSSIFAPLLFPNAFKTLSTKNKIDFHIHVVSMVQAILILFAIIPLFNDPILSEDRVFGYTPYGGLIATMALGYFLWDTIVSLVYVRYFGIGFLLHGIVSSAVFWIGIQPYIVHYAAIFILFEISTPFLNLRWFGIRFPKLFPDWFNLVNNAILILIFFFIRICYGWYQAFQLGTDFYNASNDERFNLLYTIVIMGGNSVLNVLNLYWFYRMAKVAYAVIADMLSGGNEHDDAKKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBP6</accession>
    <name>A0A1E3PBP6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_25018</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041514.1">
        <property type="nucleotide sequence ID" value="XM_019181087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBP6"/>
    <dbReference type="STRING" id="683960.A0A1E3PBP6"/>
    <dbReference type="GeneID" id="30198333"/>
    <dbReference type="OrthoDB" id="3980768at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030695">
        <property type="term" value="F:GTPase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24205">
        <property type="entry name" value="FOUR AND A HALF LIM DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24205:SF4">
        <property type="entry name" value="PROTEIN ESPINAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="26"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="147" mass="16654" checksum="A81B5129F1C46735" modified="2017-01-18" version="1" fragment="single">MTEIILDEPLSPAHNSFLDEEDDESHKCRRCDLPISEGHAYELGGDRWHMYCFSCSKCSKLLGTSSNFLVLGTGDLICSECSYACNACGKKIDDLAILTGEQAYCSSCFCCRNCKKRIEDLRYARTSKGLFCMSCHEKLLEKKRRHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBQ5</accession>
    <name>A0A1E3PBQ5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_88501</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041937.1">
        <property type="nucleotide sequence ID" value="XM_019186158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBQ5"/>
    <dbReference type="GeneID" id="30203404"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="67" mass="7235" checksum="27A4A49EBABDF18E" modified="2017-01-18" version="1">MNRLKSLHQYGIGLGDFAGYHESVIVNDGEVSILATRNFGDGVPDSIKKSDTEDLSEIIASGKIIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBX5</accession>
    <name>A0A1E3PBX5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_82836</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042123.1">
        <property type="nucleotide sequence ID" value="XM_019185449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBX5"/>
    <dbReference type="STRING" id="683960.A0A1E3PBX5"/>
    <dbReference type="GeneID" id="30202695"/>
    <dbReference type="OrthoDB" id="273181at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033260">
        <property type="term" value="P:nuclear DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14516">
        <property type="entry name" value="DSP_fungal_PPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000110">
        <property type="entry name" value="PPS1p Protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053239">
        <property type="entry name" value="Dual_spec_PTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047949">
        <property type="entry name" value="PPS1_DSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47550">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE PPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47550:SF1">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE PPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="3">
        <location>
            <begin position="538"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="4">
        <location>
            <begin position="625"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ62916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="807" mass="92139" checksum="2A217431AFF0B555" modified="2017-01-18" version="1">MNWHYSNPLPECEAIFPWLHGIHHSNQNQRDFLNSLMNFTTIEKNNFDAFNTKNKIPSTSRCLMPVRSNCIQNKHERVVKNSGVLKGSVMPEEILCPLDVSKQDLLILLNSFLKDLKIHETNEDDFENVLRILFQDCELMGLLPIFKNLDPVKGISLRNFHIQVSKLSQISDFIVYCFNDSHLDSSDSEHISCHCASVARLLYFAQLKHAIDYPELIGSMFTTLILQDNDLSFFRKTESNQRLLAIDKIDDRDTKKPIGKFCSLYDFESFNNWDTNYLFREKLEISRMSTATHVMKNVWFGNTTDFEVLKLKGPKPFERLNGDSTLPLYVDPSFSTVTVTRDRLLNDKDDEALITPAMANWKLFVNCCEGSNFPNLKLLRFLLASKSEDLINQQTHLTFPPSGSIGIGDCTDDDLISLINTCKLLYTRSSQDTPSLIYCSDGYTESSLLGVCFAIYASGEPLTKVIVDLHTLHGRPFFLFPTDVQLISRLEELLLHYSPVHSKKFEPKTLETIDKQSIYKQLYINGIPTWCTRLNGSLPSRILGHLYLGSLHHANCPELLENLGITRIVSVGEKLNWENEDELHRTIINSNISVINGIPNTPIEKVMCVSNIQDDGIDTLTHNLKDILEFIDEAYQNNGKILVHCRVGVSRSATVCIAEVMKRLKINLIRAYIYVRVRRLNVIIQPNLRFMYELVKWEESERLINSKKKLLEAKSSITNSPVDSVIPKHPISGVELSKKLSESANSPIRIASTALDQVIEDDDDGADNGSVDEDYDDSENNYWLRDVDWHILCREIDLLNKTYIGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC63</accession>
    <name>A0A1E3PC63_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_76975</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042021.1">
        <property type="nucleotide sequence ID" value="XM_019184481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC63"/>
    <dbReference type="GeneID" id="30201727"/>
    <dbReference type="OrthoDB" id="10581214at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="152" mass="17193" checksum="8B355BBBEE5077BE" modified="2017-01-18" version="1">MAVFGVKGPRGKVVEKLFQKVIELVKQDADTNIIWLEDTGTIPFQSLALNYDADEQILSRIQYIRVFDLEGYIDSFKEIKCKSVLVLSHFGAMCKDRVYQDETPRGNALGMNAWTVDHVTRYRAVTDFCESAPTSLVYGCGVNPIFFKRLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCH5</accession>
    <name>A0A1E3PCH5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_27237</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042197.1">
        <property type="nucleotide sequence ID" value="XM_019181349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCH5"/>
    <dbReference type="STRING" id="683960.A0A1E3PCH5"/>
    <dbReference type="GeneID" id="30198595"/>
    <dbReference type="OrthoDB" id="10264707at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052742">
        <property type="entry name" value="Mito_N-acetyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015416">
        <property type="entry name" value="Znf_H2C2_histone_UAS-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43138">
        <property type="entry name" value="ACETYLTRANSFERASE, GNAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43138:SF2">
        <property type="entry name" value="PROTEIN SPT10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09337">
        <property type="entry name" value="zf-H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="53"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="420"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="469"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="492" mass="55736" checksum="F1DBB68A6121EDFB" modified="2017-01-18" version="1">MTDYRPDQHFHELLPPYTVLLKDGETTATIYPIHNSTHLPPGLLAFLADEFNMEVERGDTFPFFDTLGIEEFKNYWFGAFSAVMVLGDEPQLDRQRQWEKECLGSFFIKANFPGRSSHICTGGFLVNAGIRGKGIGKTLTECFLDWAPKLGFTSAVFNLIFETNVGTRRIFESLNFKRIGKIKAAGILKGNDSAVDAIIYGKELVHPTENIQGTYRFDKIKYYLETGRYPPMIDRQEKSRLRSSASHYRLVDGKLTLNGKEVVSDPVKQLEICTQYHLVNHGGINKTTSAITDRFHWTRIKDTVAQAIRNCDECRDPTKSLAAKKKRENITLVNNRKRQRQGSQQMDGHSESPTHSHADSDPSSHLHRTDPSLLQADLTNLDDPSGIFAAVEAAQRQRSTYNDKDSYNAYQQQSRQPLRQQFDNGNNHYNQDSNNGNQRDDNDDIPVDPEVSAFDEHVQSGEEIEIARALIQANESVDPNNGQETDDNDLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU89</accession>
    <name>A0A1E3NU89_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin-like protein ARP9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_86219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035968.1">
        <property type="nucleotide sequence ID" value="XM_019186015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU89"/>
    <dbReference type="STRING" id="683960.A0A1E3NU89"/>
    <dbReference type="GeneID" id="30203261"/>
    <dbReference type="OrthoDB" id="74201at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10208">
        <property type="entry name" value="ASKHA_NBD_ScArp9-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.60:FF:000002">
        <property type="entry name" value="Actin-like protein ARP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ56761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="434" mass="49024" checksum="2C4537A3E84C35D0" modified="2017-01-18" version="1">MAPFHEEHFLIISPGSKITLVQFGIHDSTYTPPVIEVPTKVYKHPTKENFFLSKGEETDVIYPIIKGKIVNINAFNFFLKLIYKSILSKNPNINNIPFLLLSSAGWSRLDIEYITQYVFESMELNAFTVIPSSLATVYAYGAQPNGVIIDIGFDKTEITPVVDYEVVNHAKKTLDVGGNDINKQLSKLLPNLTSSQIESLKKSSIYEVLNDEDKQNSFFGKDGFHQDEDDNEFDVAAIVTSGRTREILEEREKKGENSIPNSQLEKNSFIDQDDNEITIGKERFKGCENLISTISTKVHDCLTKIVDLSKRQECWDHILIVGKTSKLKGFIDSLNAQLIEDHLIGKELQENPAQAAFQTTSTSSIVYNQIPNSIKFGKMPEYFPEWKKAGYADGFFLGGQIVSKQIFGTNNEHFFVTRQSYNDKGPLAIWETSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUB0</accession>
    <name>A0A1E3NUB0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_65600</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035925.1">
        <property type="nucleotide sequence ID" value="XM_019183948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUB0"/>
    <dbReference type="STRING" id="683960.A0A1E3NUB0"/>
    <dbReference type="GeneID" id="30201194"/>
    <dbReference type="OrthoDB" id="3978143at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12418">
        <property type="entry name" value="RRM_Aly_REF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051229">
        <property type="entry name" value="ALYREF_mRNA_export"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025715">
        <property type="entry name" value="FoP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965">
        <property type="entry name" value="RNA AND EXPORT FACTOR BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965:SF82">
        <property type="entry name" value="THO COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13865">
        <property type="entry name" value="FoP_duplication"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="67"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="150"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="150"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="186"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="211"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ56718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="235" mass="27084" checksum="944EB5EFA9CEFBA8" modified="2017-01-18" version="1">MSALEKSLDELIGDHSKPRSRDSRRPPSRPRRDRERSRERRPQSRDFRDRYEPPRQTKLQRLSHGKAYLKITNLHYEIGEQDLKSLFETVGETSFVLIEYNHSGRSSGVAYVGYEDQDNNETAVQKFDKKKAVGEIISVEEIKPLNVLIAPRGTRSGTPRGSRAYRGETRGGHRTKKPSMDDLDEELNRYMNKEDDKPQRPVKSNGKHSKPTIEDLDKELDDYMKTDQPFPATGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUD8</accession>
    <name>A0A1E3NUD8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_71339</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036006.1">
        <property type="nucleotide sequence ID" value="XM_019184238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUD8"/>
    <dbReference type="GeneID" id="30201484"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ56799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="80" mass="9334" checksum="34E5038C7AD89102" modified="2017-01-18" version="1">MPEKPQIRTIRLFNIDGHDDYFLPIELMSGSILVDRSNVPTYVEDIFEKTSDMDSQLLRLLKVEILDLGYMKNLMRKATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUQ4</accession>
    <name>A0A1E3NUQ4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histone H3-like centromeric protein CSE4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">CENP-A homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">CENPA homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_37034</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes. Replaces conventional H3 in the nucleosome core of centromeric chromatin that serves as an assembly site for the inner kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of centromeric nucleosomes, where DNA is wrapped around a histone octamer core. The octamer contains two molecules each of H2A, H2B, CSE4/CENPA and H4 assembled in one CSE4-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with the inner kinetochore.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035967.1">
        <property type="nucleotide sequence ID" value="XM_019182506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUQ4"/>
    <dbReference type="STRING" id="683960.A0A1E3NUQ4"/>
    <dbReference type="GeneID" id="30199752"/>
    <dbReference type="OrthoDB" id="842664at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061638">
        <property type="term" value="C:CENP-A containing chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000087">
        <property type="entry name" value="Probable histone 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810:SF1">
        <property type="entry name" value="HISTONE H3-LIKE CENTROMERIC PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810">
        <property type="entry name" value="HISTONE H3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="15">
        <location>
            <begin position="39"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ56760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="133" mass="15388" checksum="1EFAD0C0B2A73DAF" modified="2017-01-18" version="1">MARISTPNSSRRRRNDEGIYRNLPGDPIPTTRKKHRYRPGTKALREIRRFQKSTDLLIAKLPFARVVREVAMQYVGTELAIRWQSVAILALQEAAEAFLVSLFEDTNLLALHAKRVTIMQKDIQLARRIRGTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUY4</accession>
    <name>A0A1E3NUY4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cytochrome-b5 reductase</fullName>
            <ecNumber evidence="4">1.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_13162</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="4"/>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036143.1">
        <property type="nucleotide sequence ID" value="XM_019180561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUY4"/>
    <dbReference type="STRING" id="683960.A0A1E3NUY4"/>
    <dbReference type="GeneID" id="30197807"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090524">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NADH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF171">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0274">FAD</keyword>
    <keyword evidence="5 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="11">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ56936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="213" mass="23924" checksum="216D84823636740A" modified="2017-01-18" version="1" fragment="single">LKLASISQESPDTKYFKFRLPDHSITGVQPGKAVLAFVNDNESGKLKVRPYSLLYIPDEKEFLHLAVKLLNGDGTSGAFHRLKVGDEVTFRGPLPIHTIDYQKLSKNVNFIAGGSGITPVFSTIQNILLNHDQNVSLIYANKDQNDIPLKSKIDQLKRKYGDRFQLIYVVDKQHNFENFVSQGRINPSLLKQHLYSKDAQLFVCGPDGLTKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUZ2</accession>
    <name>A0A1E3NUZ2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">60S ribosomal subunit assembly/export protein LOC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">60S ribosomal subunit assembly/export protein loc1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_81196</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LOC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ56958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036165.1">
        <property type="nucleotide sequence ID" value="XM_019185192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUZ2"/>
    <dbReference type="GeneID" id="30202438"/>
    <dbReference type="OrthoDB" id="1743802at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037650">
        <property type="entry name" value="Loc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28028">
        <property type="entry name" value="60S RIBOSOMAL SUBUNIT ASSEMBLY/EXPORT PROTEIN LOC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28028:SF1">
        <property type="entry name" value="60S RIBOSOMAL SUBUNIT ASSEMBLY_EXPORT PROTEIN LOC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="153"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="171"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ56958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="205" mass="23991" checksum="B45393344D7E2B00" modified="2017-01-18" version="1">MAPRQSKTAKRTATQNKTRSIESEVFKDSQARNRLGFTSNQTEKSRVRKPSKKDVKKEQSKIRLYGKKKEQKIYDEKDLDLPVLNRAIVPGVKRSRGKKGKKFVNENPEDSTQLNRIINEVILKDEKRDYSKLEKAQKLEEIRELKKKEIEQKEAAKRDKLEDKKQEIKNKATKARTDRRKRAKLQTQLAEEEASKPKKKKVAFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVD3</accession>
    <name>A0A1E3NVD3_WICAA</name>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_21656</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036305.1">
        <property type="nucleotide sequence ID" value="XM_019180909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVD3"/>
    <dbReference type="STRING" id="683960.A0A1E3NVD3"/>
    <dbReference type="GeneID" id="30198155"/>
    <dbReference type="OrthoDB" id="3973241at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF12">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="177"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="27"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="208" mass="23591" checksum="6BB5181071C09B1E" modified="2017-01-18" version="1" fragment="single">MNRLFGSKNNVPKPTLNDAIGNIEERVSSLDIKLSKINAELSAYQQKMSRMRDGPGKNSIKQRALNLLKQRRQIEAQKDQLESQSWNMSQAQMTTDNLKNTMVTVDAMKQTNKQLKKTYGKIDVDKIEDLQDEMLDLIEKSNDLQDALAQSYDVPDEISESELDAELEALGDEIDYENEMSEATGVPSYLQQDSNVPQFIDEPVDDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVN4</accession>
    <name>A0A1E3NVN4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_21433</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion inner membrane</location>
            <topology evidence="1 13">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the TIM50 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035959.1">
        <property type="nucleotide sequence ID" value="XM_019180904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVN4"/>
    <dbReference type="STRING" id="683960.A0A1E3NVN4"/>
    <dbReference type="GeneID" id="30198150"/>
    <dbReference type="OrthoDB" id="287041at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046902">
        <property type="term" value="P:regulation of mitochondrial membrane permeability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000019">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit TIM50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10 13" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="FCP1 homology" evidence="15">
        <location>
            <begin position="150"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ56752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="421" mass="48829" checksum="A24FDFA69327014F" modified="2017-01-18" version="1" fragment="single">KDKKKEQQQSILTDDLLAKAGVDVEEPTASKSNGKQEGESSSSGETEETAGNEDGKSERKKRNRQSSTDVKREKAANYFYLGFFATALGTVGYLARDWEADEDEKIKKDIPNGYSLDSMWARFNARFNSIFTYFQEPPFPDLLPDSPPEPYKRPLTLVLTLEDLLIHSEWDSKNGWKTAKRPGVDYFLGYLSQYYEIVLFSSNYMMYSEKIVEKLDPLHAYISYQLYKEHCLYKDGAHIKDLSKLNRDVSKVLIIDVDENSIKLQPENAIKLQPWNGQADDELIKLIPFLEYLATQQVKDVRPILSSFKNKSHISEEFLERSEKLRQEFEKDQAKKESTNLLNKLLGLPVVKQKFPLDAIHDEGVRNFQHFQQLIKEEEQKLKLQAEMPKETFKLKDIVEGNLPSQEEMMKKQLEKQLEFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW23</accession>
    <name>A0A1E3NW23_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_36909</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036099.1">
        <property type="nucleotide sequence ID" value="XM_019182486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NW23"/>
    <dbReference type="STRING" id="683960.A0A1E3NW23"/>
    <dbReference type="GeneID" id="30199732"/>
    <dbReference type="OrthoDB" id="27483at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030289">
        <property type="term" value="C:protein phosphatase 4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072542">
        <property type="term" value="F:protein phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051598">
        <property type="term" value="P:meiotic recombination checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000002">
        <property type="term" value="P:negative regulation of DNA damage checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902660">
        <property type="term" value="P:negative regulation of glucose mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001034">
        <property type="term" value="P:positive regulation of double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055236">
        <property type="entry name" value="EVH1_PP4R3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006887">
        <property type="entry name" value="P4R3-like_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051137">
        <property type="entry name" value="PP4R3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23318">
        <property type="entry name" value="ATP SYNTHASE GAMMA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23318:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22972">
        <property type="entry name" value="EVH1_PP4R3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04802">
        <property type="entry name" value="PP4R3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PP4R3 EVH1-like" evidence="5">
        <location>
            <begin position="11"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Serine/threonine-protein phosphatase 4 regulatory subunit 3-like central" evidence="4">
        <location>
            <begin position="148"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="702"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="708"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="755"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ56892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="793" mass="91347" checksum="40AA1EE69981A890" modified="2017-01-18" version="1">MTKTPKHQALRRVKVYLLEDAWIDNGTGYCFGEIDEDKVPYLIVRNELDQKDVLLKAKIEGSIQFQRQQDTLIVWTDTKGHNIALSFQEKEGCLSLCDFIIKCHRGKFAPLISLIAVISNDMDGDITELITGPINYPPLEPTAGDLIEISNCFNENPNSSYQKQSISNFIKNGNYISKLIDIFNESEQNRKIKNLHLLCNIVKTLILFNEGLIIEMILDDENIMGVVGMLEYDPDFPQFKSNHRSFLRDDSKFKEVLPVKDEHIKKLIKKTFKLQFLKDVVLARLLDDPTFNLISTLIHFNQVEIIEFLEKGTFIDELFKLYQDEDNTEDTTIQKRDGIKLLHQFILIAKNLQPHQKTSFFKSLIKKGLFKTINFVMKDNSTEIRVIGTELIVTIIEHDVLLINGVVDDEEEEGEEVNDDDNDDNDDFKHGNGNFFNGFDYEQDEEPPHITYRHREIVLSDDMTLLIILTQFLLEDNDPGLKIQAFEALKSLLDPVNNLNSAIMNNDDSMIDQLDATKFFKAFYDKVAPILFKPLMNIERKDDNQHNDELFIYLCELINFCSKDKSISRSFFLENHILIGITQLIKPTHKLQLRLAAVRSIKSIILLNDDYYTRYIISNNILEQVFELFDETNGISNLANSTVLDLLEMILLGIDKLDKRKNVKLLATYIVSNFKASLDSVDSVSSGKDLIKFAEQDVVNTSTTTNELNDDENYEDDDTLTRPPEISTEPLDEEDVLNNEEILKEKSLNRTSNKVPEKRSREEDFESSHEIKKKNSLREKFSNAGKKIASKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWF5</accession>
    <name>A0A1E3NWF5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vacuolar protein sorting-associated protein 62</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_36208</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036537.1">
        <property type="nucleotide sequence ID" value="XM_019182406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWF5"/>
    <dbReference type="STRING" id="683960.A0A1E3NWF5"/>
    <dbReference type="GeneID" id="30199652"/>
    <dbReference type="OrthoDB" id="188042at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009291">
        <property type="entry name" value="Vps62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053102">
        <property type="entry name" value="VPS_Associated"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48220:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06101">
        <property type="entry name" value="Vps62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="402" mass="46646" checksum="75669127DF95CB75" modified="2017-01-18" version="1">MNYVHNELPNWQLKELPPIKKFPIMQTDRTLKKGEIPKYVLDHNPLVHLYTEEEYLPYDIEDYVKHFHLQFRNQSIIQNSSLSLQELGEYSHNSSIDTSETFLTCDFEFKDDPEWLTGKRNKPNLSTGLIKDAPSVLIVVDKGNGWVDAYWFYFYSFNLGPFVMGGGPYGNHIGDWEHSLTRFFHGVPIFVWMSAHGGGGGYHYKALEKSDVDSRKPIIFSARGTHANYASVGQHSHDLPFGMLSDFTDRGPLWDPSKNYLGYTWDGSNLTPVNKESKPDNQRELEYGDWLLYKGHWGDDKLDPKDPRQRWSPFEWKYIEGPTGPLTKHLNRVKLCQRSKWWNFWHGCPPKNFIKVGEGVEKEAGVVCANLFGWVRPALLRDFLENLTWGGGLCYIMNWRWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWK5</accession>
    <name>A0A1E3NWK5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">F-actin-capping protein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_70503</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the F-actin-capping protein alpha subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036587.1">
        <property type="nucleotide sequence ID" value="XM_019184209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWK5"/>
    <dbReference type="STRING" id="683960.A0A1E3NWK5"/>
    <dbReference type="GeneID" id="30201455"/>
    <dbReference type="OrthoDB" id="340550at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008290">
        <property type="term" value="C:F-actin capping protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110085">
        <property type="term" value="C:mitotic actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.60">
        <property type="entry name" value="F-actin capping protein, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.210">
        <property type="entry name" value="F-actin capping protein, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002189">
        <property type="entry name" value="CapZ_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037282">
        <property type="entry name" value="CapZ_alpha/beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042276">
        <property type="entry name" value="CapZ_alpha/beta_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042489">
        <property type="entry name" value="CapZ_alpha_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017865">
        <property type="entry name" value="F-actin_cap_asu_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10653">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10653:SF0">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01267">
        <property type="entry name" value="F-actin_cap_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00191">
        <property type="entry name" value="FACTINCAPA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90096">
        <property type="entry name" value="Subunits of heterodimeric actin filament capping protein Capz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00748">
        <property type="entry name" value="F_ACTIN_CAPPING_A_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00749">
        <property type="entry name" value="F_ACTIN_CAPPING_A_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0117">Actin capping</keyword>
    <keyword evidence="4 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="249" mass="28434" checksum="6243F181696764C7" modified="2017-01-18" version="1">MSSVDKIISDIIKDTPVGELPKVIEDLKLIIDKNINHELSIAIKEYNLTNFTSIDVENNPIIISKYNQDDDEFFIDSKNGVKFKVDHLLLKPQEITKIEQSISKYDSELSKYVIDQYNQGEYLINNDVILIKGHKTSSLNYWTGSWRSKYDLQTGKGEINIDVHYYEDGNVRLQTLQNVEIDISSPISSIKQVETKIQLSLNKQFANLNEAVFKQLRRQLPVTRSKINWGKAIGNYKLGKLTSSSSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWU8</accession>
    <name>A0A1E3NWU8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_97011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036825.1">
        <property type="nucleotide sequence ID" value="XM_019186548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWU8"/>
    <dbReference type="STRING" id="683960.A0A1E3NWU8"/>
    <dbReference type="GeneID" id="30203794"/>
    <dbReference type="OrthoDB" id="10584940at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="181" mass="20457" checksum="DF4DBF7D2FC0F7CD" modified="2017-01-18" version="1" fragment="single">MNQKEHITPPKRFRPTSIISNPSTTSSHSNLNNYPVGLIKVYQSDEEFSNDSFSITSRKLNDARSIISQVDPTHAHNFSTTSSLSETNDVSYDTAPTIDDDSLFNLWFQTPEKERESLVIPSGGLERPRYVLDKVVESFRSEKGAFLTSDIFLNQKIYQQPETIINGSVKISIIQEINTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWW4</accession>
    <name>A0A1E3NWW4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase class V domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_96838</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036707.1">
        <property type="nucleotide sequence ID" value="XM_019186541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWW4"/>
    <dbReference type="STRING" id="683960.A0A1E3NWW4"/>
    <dbReference type="GeneID" id="30203787"/>
    <dbReference type="OrthoDB" id="5978656at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43092:SF2">
        <property type="entry name" value="HERCYNYLCYSTEINE SULFOXIDE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43092">
        <property type="entry name" value="L-CYSTEINE DESULFHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="2">
        <location>
            <begin position="65"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="432" mass="49862" checksum="DF5FA361D45873DF" modified="2017-01-18" version="1">MTKDSDLIEFGRPLREKYFSHVNPEAYLINNGAFGLAPLPILESLQRHTLRDYYWPDAYRKFEYRDDYDDSAKEVAKVLNTDYRNIALGLNVTTLINAVLRSFKFEKGDKILVQSNTYPAILKTVNFVAKQNELEVVKFDLNYPLSDDEVVSKYEDELSTNKIKIVLIDTISSKPGVRVPFERLIKLAKEYDALSLVDAAHGIGLLDIDLDELKPDFLATDLYKWYYTPKNSAALYVDPKHHKAIHSLPISHFYVDGDQVLKEEKEQTRFADQFNFIGAQITASLVTVKDASKFIEEIGGLQRVKDYNFRLAREASEAVAKLWGTEILENDEKSLLTSLFTVEYPVKEGVLIREEDWENLEKYMLIRQTIEKKLIKEYKSVAPIFLHNGKIWIRFSAQIYSELNDYIEGAKLLEKGVDEFFASEEYKKLIAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX18</accession>
    <name>A0A1E3NX18_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Carboxypeptidase</fullName>
            <ecNumber evidence="14">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_34852</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential release of a C-terminal arginine or lysine residue.</text>
            <dbReference type="EC" id="3.4.16.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="14"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036890.1">
        <property type="nucleotide sequence ID" value="XM_019182243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX18"/>
    <dbReference type="STRING" id="683960.A0A1E3NX18"/>
    <dbReference type="GeneID" id="30199489"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF190">
        <property type="entry name" value="PHEROMONE-PROCESSING CARBOXYPEPTIDASE KEX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4 14" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012949683" description="Carboxypeptidase" evidence="15">
        <location>
            <begin position="16"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ57683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="463" mass="53117" checksum="588CC30219025F38" modified="2017-01-18" version="1" fragment="single">MRLPLLLFLITGVLPSPIDIPSKQFIVEDLPGIDQIPKDERPIMYSGHLELNSTIDENYFFWKFIKPSTQFNRTIFWLNGGPGCSSMDGALMESGPFRVDENENLYMNKGSWHEVANLVFVDQPGGTGFSITKDYDKDLIRVGEDFVVFLEKYFQVFPEDLNNEIFISGESYAGQYIPFIADTIFKSDKEFAKNLKGLIIGNGWIAPNIQSLSYIPYLLDVGILHTSDEFMPRLLKQQEHCQNLVNNPVNDDKFSIYECENILTRILQYTRDKKAPKDQQCINMYDIGLKDSYPSCGMNWPPDLKYVQPYLQKDQVVESLNLNVSRVKKWRECDGDVSKFLKNKKIKPSFNLFPSLLEKVEIMLFNGGHDIICNNYGVLDLINKLHWGGSTGFSPDTEEFIWKYNDELTGSVKTSKNLTFVDVYNSSHMVPFDLPEKSRGVFDLFVKNFDIIDKDGSSFIQTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX68</accession>
    <name>A0A1E3NX68_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_97882</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036459.1">
        <property type="nucleotide sequence ID" value="XM_019186614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX68"/>
    <dbReference type="GeneID" id="30203860"/>
    <dbReference type="OrthoDB" id="416217at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF12">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="8"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="783" mass="90111" checksum="395EEEB00C78589B" modified="2017-01-18" version="1">MSNNMNAVCIGCRLRRKKCDRGKPICTSCLELNISPDLCIYPKSKLQLASKDSKITLDLLRSKNKELKEEYEAVKAGEKPSTNSLQNNNVVLSSQNNPSDKIDNTPINPSAEVLGFVQDPINTTVKETNEFLLHCGPLSWVSLMSFEKDFAQLCLQFGQIIAIAKDLRKDSRRGEFIDQADSDTRFNDIKSRLSRILLGIDPNENIFKVMEELFLEIENRLPHYDMVMTLIDRFFITTRIQKMGVIQLNEDSFYETLSQIITKDSNGRVKITVKFPDEYYKTPGVSLLLSIVATMLFQVNQLVNPITSANHLLILDCIKVLFVGFSIFKRTSPTPEAVVSPELAVQLLQGFLYYVGFDRYNPHGREHEKADKTGENAICVRKMVSYARALKLNCDIEKIYRHKSKNYRRSLKKSIEFGLPTKIKRDELYCYDDCQDEYSKSVIVLNELIADFQNIEKISNTLDFIQLVEKNLITKAKNTLRDLFKPIHEDLKTLNEMNFDDLSLVHKFMAITQTFNVRLTLLTLIETYYHHCFARLKQSGYDNTDFCKKYQILAMKYSLLVFQVISGIFSAYRRLWGHENSTTFGSLGTILAIFSTMKFTFRRVTIFAGARVFEGLPVEKSTVILDLFELGKETDSEILNYLLRTQKHPFFTIADLENGVVDNHDEILLKKFSILTDYRFSMLSAAVSMYNLAESLNNSRYDLNFVRLNRAFYFALKLVSFFTRLEYRNEVYGKDSLTSGKNKKNTINEVNLEEFYRQAISTKSNDALDLEIFFSAPNSSGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXF5</accession>
    <name>A0A1E3NXF5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_80056</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037096.1">
        <property type="nucleotide sequence ID" value="XM_019184997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXF5"/>
    <dbReference type="GeneID" id="30202243"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ57889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="232" mass="26440" checksum="E3D091D1234540FD" modified="2017-01-18" version="1">MNNIRNTPTDITNQITPPSAFATLLTEEETGFLASEYFNAVEFGTEDLDIGDRPPFELNSSDASFNNRLLNGAFGPIPEIDEEMYIRYDPRAVENPDENDVAYWITENNQENDPGLLANSLALARERELQRARHNHTTRTTRAPNWHISVYGHPSFINDVERRTVRPPPAPAPVYVHGRNTTPRTSTLDGFDNYAHLGYLEPGLEERRRSWKGIREMFGVYDDSNDENTTPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXK9</accession>
    <name>A0A1E3NXK9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GPI transamidase component GPI16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_57667</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036619.1">
        <property type="nucleotide sequence ID" value="XM_019182810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXK9"/>
    <dbReference type="STRING" id="683960.A0A1E3NXK9"/>
    <dbReference type="GeneID" id="30200056"/>
    <dbReference type="OrthoDB" id="331263at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007245">
        <property type="entry name" value="PIG-T"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12959:SF11">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12959">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-T-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04113">
        <property type="entry name" value="Gpi16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133553" description="GPI transamidase component GPI16" evidence="2">
        <location>
            <begin position="20"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="537"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="603" mass="67900" checksum="F95CF85EA53EB8E1" modified="2017-01-18" version="1">MRSFVSFIWLLFAAALSNAAEDPNEEVKDKVNDKVESINETTYPYRESLDITPLPRNNLLVSFNFELESNYVNVSKTPGHLHYTTFPRSIAPILEKTNTRELHLRFGQGWWDSEEWGELPLSGQYSGGVGVEVWSVIEAADRKESFESWKYLVNSLSGLFCASLNFIDSSKTTFPIQSFKPKGPVTLLNEKNELHLLRAALASEPVCTENLTPFVKFLPTRGKSGLSSLLDGHRVFESEWHKMSIDIETECEGDLCKYTMTQKIDAVVDVSRALRRKKNPIPKPASGDDLRCDLSKPYDAFQCFPLGDPTDLTFTLSDLFNRQISGGNLLSSTSSKVCTHVSDKWKTFLKVDGQHFGTPSNCFDINDSRDYDIYFHTDDSTDVLQTQNAPIFVSRSLTGYGQDKGGSRTVFKNPSDSTVLLVYFESLPWFMKVYLHTLSVNGAEMKEVIKSIYYSPEVDRERPSQIEFELLIPPNSNIALSYAFDKSLLLYSEYPPDANHGFSVEPGVVNVLEPTIYSLRTSSALLTLPTPDFSMPYNVIILTSTIMALAFGTLFNLLVKRVVTEKEAELIIEETGPKAVIQKFKQRIRLSLKSKSPSDQLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXL1</accession>
    <name>A0A1E3NXL1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Allantoin permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_33654</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037051.1">
        <property type="nucleotide sequence ID" value="XM_019182101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXL1"/>
    <dbReference type="GeneID" id="30199347"/>
    <dbReference type="OrthoDB" id="2018619at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
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        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF15">
        <property type="entry name" value="NICOTINAMIDE RIBOSIDE TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="378"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="402"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="447"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="485"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ57844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="561" mass="62100" checksum="0179938C9128E83A" modified="2017-01-18" version="1">MTLDIRKLRIREGLKKRLEVPTDQDAEYSNDSLTNRDIIPIPPERRTWKPFAYAQYWIVEGISITGYTQASSLIGIGLSPKQSILCALAAGIIYGFFAALMGYIGGKSHIGFPVLARMVYGIKGSVFGIVIRIITGLIWLGVQAMYGGRAVSYMLATFSPSFAKWDSFNNANGITSNIFVGLVLYYIFLIPCVYIPPEKIHLFFRIATILILGTFFGMLGYSVKTAGGVGTMFHDPPQTFKTRGELGWACVKGIFSIVGSAGTGILGQSDFTRYSSTKRGPILPQILGAPIALTFSCVIGVITTSASSQFLGEVEWNPTVLLNKIQQYEGNSSKARAVIFFGCFSFTLQQMAINLMLNCLSSSMDMVGLCPRYINIRRGSILIMAVSILIWPWKILTSAKAVVVFGSGWGVFCSAQTGSFIVKYFIVYKRKLLLKDLYIKSNESIYWFYNGVDWRAIASFLVGTVFLIPGLVWDTLDKSNGFWTWIYKVSYASGILISMASQLLLELVFPKKTIQPTTKDDDIYNEDGSRVSCVIDGQSLKDFELVVNVDNKVLDAHGKYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXT5</accession>
    <name>A0A1E3NXT5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_35309</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019182292.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3NXT5"/>
    <dbReference type="GeneID" id="30199538"/>
    <dbReference type="OrthoDB" id="5784at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:inositol phosphoceramide synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045140">
        <property type="term" value="F:inositol phosphoceramide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006676">
        <property type="term" value="P:mannosyl diphosphorylinositol ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="PAP2_Aur1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aur1/Ipt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052185">
        <property type="entry name" value="IPC_Synthase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310:SF11">
        <property type="entry name" value="INOSITOL PHOSPHORYLCERAMIDE SYNTHASE CATALYTIC SUBUNIT AUR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14378">
        <property type="entry name" value="PAP2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="7">
        <location>
            <begin position="177"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="412"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="415"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="431"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="448" mass="50273" checksum="F06138EC901A3C93" modified="2017-01-18" version="1">MAQLFQKYFLSERPTGVVIADIDLNFNPKVSIERLKSHQFTRNEVIHFGTLIFFFSIAFFINPVNVFLKLMVVSLLITVLLIPFTSQFFLNALPILAWLAFFFTSTGIPPSYRPPISVKVLPAVETILYGDNLSQLLATSTSSFLDILAWLPYGIIHFSLPFVVAGLIFLWGPPTALRSFAFAFGYMNLIGVVIQIVFPAAPPWYKILHGLDPANYSMLGSPGGLARIDTLLHLDLYTTNFSKNSPVVFGAFPSLHSGSATMDALFLSWLFPKFTPLFVVYITWLWWSTMYLTHHYFIDLMAGSFLSFVVFQYTKYKHLPINDMNAKNRWSYSQIIKPNIYAMDPLSLAPEVDIENQISYSSQRAVNNVELNVISRHSSGTSVVQGPNAIYNDSDSTSSPALSVFETDDVPRSFSRASNNSNTSIESQFDMSIPIMPPNSSSSKSRMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY38</accession>
    <name>A0A1E3NY38_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pheromone-regulated membrane protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_85167</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ThrE exporter (TC 2.A.79) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037220.1">
        <property type="nucleotide sequence ID" value="XM_019185853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY38"/>
    <dbReference type="STRING" id="683960.A0A1E3NY38"/>
    <dbReference type="GeneID" id="30203099"/>
    <dbReference type="OrthoDB" id="413008at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010619">
        <property type="entry name" value="ThrE-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051361">
        <property type="entry name" value="ThrE/Ser_Exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024528">
        <property type="entry name" value="ThrE_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31082">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31082:SF4">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06738">
        <property type="entry name" value="ThrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12821">
        <property type="entry name" value="ThrE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Threonine/serine exporter-like N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Threonine/Serine exporter ThrE" evidence="9">
        <location>
            <begin position="224"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="383" mass="41044" checksum="9C153EAC29DC664D" modified="2017-01-18" version="1">MIVSFGDATTRTSEVQLVRCPQGLNLYKLHRVHRIYKQVIHDLVGVEEASNDLDDLLSSKPAFPPWLCVLIYAFSSAMVTPFAFGGGWVNLPVSFLIGLCVGSLQFLIAPRSNLYSNVFEVTAAIVVAFVGRALGSISGSHICFSAVVQGSLALILPGYIILCGSLELQSRNLVAGAVRMFYAIIYSLFLGFGITLGAALYGWIDKNATSETTCAEQISPWYRFIFVPFFTIGLCLINQAKWFQLPVMLFISCAGYVVNFFASKHFQNSTEFTAAMGAFVIGVLGNLYSRIWKGLAVSAMLPAIFVQVPSGIASQASLLAGVQSANQLTTNSTSGAATAPAEGSSLSFGVTMVQVAIGISVGLFASTIFVYPFGKKSTSIFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYB9</accession>
    <name>A0A1E3NYB9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_64247</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion inner membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037310.1">
        <property type="nucleotide sequence ID" value="XM_019183695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NYB9"/>
    <dbReference type="STRING" id="683960.A0A1E3NYB9"/>
    <dbReference type="GeneID" id="30200941"/>
    <dbReference type="OrthoDB" id="344165at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042719">
        <property type="term" value="C:mitochondrial intermembrane space chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140318">
        <property type="term" value="F:protein transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="3 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0811">Translocation</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Tim10-like" evidence="8">
        <location>
            <begin position="21"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="88" mass="9854" checksum="7A0F3FB0CF31FCC0" modified="2017-01-18" version="1">MSLNQADLANLDESSKKEILQFIESENSKTKVQTSIHQFTDLCFKKCVDSIGNGQLSSNEESCLTNCVNRFLDTNIRVVQGLQNAQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ61</accession>
    <name>A0A1E3NZ61_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nitrogen permease regulator 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_33631</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NPR2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037703.1">
        <property type="nucleotide sequence ID" value="XM_019182095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ61"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ61"/>
    <dbReference type="GeneID" id="30199341"/>
    <dbReference type="OrthoDB" id="338854at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015824">
        <property type="term" value="P:proline transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000785">
        <property type="term" value="P:regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015840">
        <property type="term" value="P:urea transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009348">
        <property type="entry name" value="NPR2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12991:SF10">
        <property type="entry name" value="GATOR COMPLEX PROTEIN NPRL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12991">
        <property type="entry name" value="NITROGEN PERMEASE REGULATOR 2/TUMOR SUPPRESSOR CANDIDATE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06218">
        <property type="entry name" value="NPR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="326"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="334"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="542" mass="61783" checksum="9DF2DD0E8B9FB520" modified="2017-01-18" version="1" fragment="single">GFAPIEAIFYSVFHPTEGTKVSQQVPLGAIVTPPKSTSDVLAEPLFDFDLVKNYVIPKAQLCNRLVTLKVNQYRIIGYPVTIIAPHYARNSFSFNFCFVFRYESDTTPYESAIRRLGRMFKNLEEQYQLLSRKEKDRLYFKDDGRNASTNTAITSPPMTESFNNDTSYTIKLDSIESLIQQIYQDLNNYSECLIPIDSGNSVDIKLFPLLPPPPRLNQEDVPISTVHLESMVDVNWDPTMLKILPFINGINSIKKISVLANSDYNIVKQCIQHLIYYKCIIIIDIFQFSNIYAPTSDIVLFLKDSHMATECQAYVVSPSIFKDKPFESDKSADQKTSTRTTKTPNRKSSGKDVSSVSLVGDSNRSKRETVVLPSKAKLYFLYRSLHQGQTVKSWYKEHAEALEHIDIRRFLSFGVLRGLIYRVHSYPIIDSVLLKEEHSQSKSLFLQAYNDKDLDEDNEDDEGEGTNETDAVTNLQTVKAASLFKRNTDNELSEADKRKTAIILKNLNDIKNFDAVCTELEMDRKSVETLLRKIGHFNVVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ69</accession>
    <name>A0A1E3NZ69_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Malate dehydrogenase</fullName>
            <ecNumber evidence="3 12">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_34656</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3 12"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037497.1">
        <property type="nucleotide sequence ID" value="XM_019182213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ69"/>
    <dbReference type="STRING" id="683960.A0A1E3NZ69"/>
    <dbReference type="GeneID" id="30199459"/>
    <dbReference type="OrthoDB" id="4069699at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01337">
        <property type="entry name" value="MDH_glyoxysomal_mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000013">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000009">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010097">
        <property type="entry name" value="Malate_DH_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01772">
        <property type="entry name" value="MDH_euk_gproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540">
        <property type="entry name" value="MALATE AND LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540:SF16">
        <property type="entry name" value="MALATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="13">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="14">
        <location>
            <begin position="147"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="7"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="117"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ58290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="333" mass="35708" checksum="B011D783F027B283" modified="2017-01-18" version="1">MKVTVCGAAGGIGQPLSLLLKLSPYVTELSLYDVVNSPGVGADLSHIDTNTKIQSFLPDNDGLSKALQNTDIVIIPAGVPRKPGMTRDDLFAINAGIVRDLAQGVASFAPDAFVLVISNPVNSTVPIFAEVLKKNGVFNARKLFGVTTLDLVRANTFISQLSNDSLNPENLKIPVIGGHSGDTIVPLFSLGSPEFYKSLSIEQRDALIHRVQFGGDEVVQAKKGMGSATLSMAYSGFKLAEELMKAIKGDSSIVDSSFVYLSDDIKGAKEVRQLLPDLDYISLPVKLSAKGVESIEIDVLNKINDHEKELLNVAIDQLKTNISKGINFIKNQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZM6</accession>
    <name>A0A1E3NZM6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_80299</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037349.1">
        <property type="nucleotide sequence ID" value="XM_019185033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZM6"/>
    <dbReference type="GeneID" id="30202279"/>
    <dbReference type="OrthoDB" id="4096620at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="104" mass="11468" checksum="BE476F75E4C0C34B" modified="2017-01-18" version="1">MTVQRGSSVRGTNYTKPRPFSTNNPFRNASYDSNIGSRTASQNSEQQQHFNNWVAKNQSQLSFSSDDEDGDLFKVPQSIGRPTYQRTQSNSSAGSAHSARGIPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZT2</accession>
    <name>A0A1E3NZT2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein-sorting-associated protein 36</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">ESCRT-II complex subunit VPS36</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_70437</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the endosomal sorting complex required for transport II (ESCRT-II).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the VPS36 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037731.1">
        <property type="nucleotide sequence ID" value="XM_019184205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZT2"/>
    <dbReference type="STRING" id="683960.A0A1E3NZT2"/>
    <dbReference type="GeneID" id="30201451"/>
    <dbReference type="OrthoDB" id="271448at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13227">
        <property type="entry name" value="PH-GRAM-like_Vps36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021648">
        <property type="entry name" value="GLUE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040608">
        <property type="entry name" value="Snf8/Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037855">
        <property type="entry name" value="Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031558">
        <property type="entry name" value="Vps36-NZF-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13128">
        <property type="entry name" value="VACUOLAR PROTEIN-SORTING-ASSOCIATED PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13128:SF12">
        <property type="entry name" value="VACUOLAR PROTEIN-SORTING-ASSOCIATED PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04157">
        <property type="entry name" value="EAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16988">
        <property type="entry name" value="Vps36-NZF-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11605">
        <property type="entry name" value="Vps36_ESCRT-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51495">
        <property type="entry name" value="GLUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="GLUE N-terminal" evidence="6">
        <location>
            <begin position="10"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="593"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="596"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="626" mass="71180" checksum="CED6D36F0EA0EF82" modified="2017-01-18" version="1">MGQLPYWHSVELSAGARPILREKEVDVLVENSIGLYQGKSKILNRQNGRIYMTSQRLIYIDNEDSKKYSVAIELADIANIDYTPKFLKSSAKITIFFKKLKKQKAKKITNQSIRTTTWICPICTFPNSVQTNINPNNTEYLPVCETCGIKSTSEIIQDALNSSKLKEPTTPHKPLAPKQIACPTCTFLNHPSLRNCELCGTNLPSNQENDDEVSNVDELQLFDDRIEIRTESNDGLEESKSPFIKLSFHKSGDKFFHNKLNENLEKFNWDLVLNSGNVNKDSTKVLNNDNQDIIQPQSSGSTLGFGIHSLAKQQELKTFKNEQIINSSLDDLQALLNKANEILELTESFKKLSNTDNNLHQRSLTTINQVTKDLLYDSNNESQKKLYLQEISRQLSDFLINDSVLEQQGGIVTLLDLYALFNRGRIGINLISPEELFKSCEFFKSLKLPLILKRINNVLVVQDLKFGFEDGKLIEDIKIFIKNFGDEDQFDFKFANILKISDAFNWSITITEEVLKIGLNLGELVVDQQISGKFYYLNEFKQLDSFNENRYEETEIQANIDDDGSNNDQLIEKSDNKASNTFIFPEAPNLQPYPEASTQSIGQNDSVKSSTNHKSKSLLQLEGLQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZW6</accession>
    <name>A0A1E3NZW6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_32779</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:38664"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037910.1">
        <property type="nucleotide sequence ID" value="XM_019182003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZW6"/>
    <dbReference type="STRING" id="683960.A0A1E3NZW6"/>
    <dbReference type="GeneID" id="30199249"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005548">
        <property type="term" value="F:phospholipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030011">
        <property type="term" value="P:maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000007">
        <property type="entry name" value="Oxysterol binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2720:FF:000001">
        <property type="entry name" value="Oxysterol-binding OBPalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF102">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="10"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00050362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="430" mass="48867" checksum="92E7756DF7B320F5" modified="2017-01-18" version="1">MPLSKLKMKLTDSTSSSSSVAQNSNNVNTEDIDEVDDQGQSILMGIISQLRPGCDLSRITLPTFILERKSMLERITNQLQQPDILLEAHATKDYIQRFLLVVKWYLSGWHIAPKAVKKPLNPVLGEFFTAFWDLPNDAGQAYYISEQTSHHPPKSSYFYMIPNKKIRVDGACIPKSRFLGNSTAAMMDGVTVLKFLDFIDENGEFEKYTLTQPNMYARGILFGKMRIELGDHMIIKCPNTGLSIDIEFKTKGFISGTYDAIEGVVKNSNNESLFEISGKWNDVVYIKDLINGGSKKTFFDATKATPLPPKVRPLNEQGEFESRRLWKKVTDALGERNHEVATDEKFKIEDAQRVASKKRLEDGVEFHPKLFKSVENDDLEYYLYKHIPPDADPQTITKLILETAPILPGQQFTPEFDIPAYKKLHNDDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZZ8</accession>
    <name>A0A1E3NZZ8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">DNA polymerase</fullName>
            <ecNumber evidence="21">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">WICANDRAFT_63181</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Catalytic component of DNA polymerase delta (DNA polymerase III) which participates in chromosomal DNA replication. Required during synthesis of the lagging DNA strands at the replication fork, binds at/or near replication origins and moves along DNA with the replication fork. Participates in leading strand synthesis during replication initiation and termination. Has 3'-5' proofreading exonuclease activity that corrects errors arising during DNA replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 21">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 21">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 21">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="21"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037882.1">
        <property type="nucleotide sequence ID" value="XM_019183503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZZ8"/>
    <dbReference type="STRING" id="683960.A0A1E3NZZ8"/>
    <dbReference type="GeneID" id="30200749"/>
    <dbReference type="OrthoDB" id="2414538at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140445">
        <property type="term" value="C:chromosome, telomeric repeat region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904161">
        <property type="term" value="P:DNA synthesis involved in UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903459">
        <property type="term" value="P:mitotic DNA replication lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006278">
        <property type="term" value="P:RNA-templated DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05777">
        <property type="entry name" value="DNA_polB_delta_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05533">
        <property type="entry name" value="POLBc_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.730:FF:000004">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000004">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.730">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025687">
        <property type="entry name" value="Znf-C4pol"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14260">
        <property type="entry name" value="zf-C4pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 21" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 21" id="KW-0235">DNA replication</keyword>
    <keyword evidence="17 21" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14 21" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15 21" id="KW-0408">Iron</keyword>
    <keyword evidence="16 21" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6 21" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18 21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 21" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 21" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="23">
        <location>
            <begin position="247"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="22">
        <location>
            <begin position="544"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="domain" description="C4-type zinc-finger of DNA polymerase delta" evidence="24">
        <location>
            <begin position="1015"/>
            <end position="1087"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00055225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF14260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="ODQ58675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1109" mass="125920" checksum="C1A556C10EE617E8" modified="2017-01-18" version="1">MSDNSKRALNEVENIDPTSKKAKIEIGIDPTPDGTVEELTKEAHSLPVSLQSTQDDQHLHQSQTFKAKNAMAQVEPSKFEKELLSMSNDINIKNLTAEDQTWERPSLPKDWDPNTHDITFQQLDAEEALTDGSSAVRFFGVTQEGYSVLCNVTGFLHYFYVPAPMDFRKEHVSTFTHYLQQTFSGVHSVGLAFKESIWGFNGNQKVCFLKIVGVNAKDIPKIRSGFEKGMVSWNGMFNNGGDGTMTYDNIQYLLRLLIDCKITGMSWITLPKSTYTIINSHSKLSRCQLEVNIDYHDLVSHQPEGEWLKMAPLRILSFDIECAGRKGIFPEAEHDQVIQIANVVSRSGESRPFVRNVFTVNTCSPITGSEIFEHQDERDMLLAWRDFVTRVDPDVIIGYNTTNFDLPYLLDRANALGLKDFPYFGRLKNIKQEAKDSVFSSKAYGTRESKVINIDGRMQLDLLQFIQREYKLRSYTLNSVSAHFLNEQKEEVQHSIITDLQNGTSETRRRLAVYCLKDAYLPMRLLEKLMCLVNYTEMARVTGVPFSFLLTRGQQIKVISQLFRKCLEVDLVVPNLQQSGSSDVQYEGATVIEPHRGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLNKPTIERLSLRKDEDYTVTPSGDFFVKSTQRKGILPIILEELLSARKKAKADLKKETDPFKRDVLNGRQLALKISANSVYGFTGATIGKLPCLAISSSVTAFGRGMIEKTKQEVQQKYTIANGYEFDSEVIYGDTDSVMVKFGYKDLETCMKLGEEAADYVSTKFLNPIKLEFEKVYFPYLLINKKRYAGLYWTRTDTFDKMDTKGIETVRRDNCRLVSNVITKILELILEKRDVKSAESYVKQTIADLLQDRVDMQQLVITKALSRQDYANKQPHVELAERMRKRDAGSAPTIGDRVAYVIIKTAGNKSYEKSEDPLYVLEHNLPIDAKYYLENQLANPLTRIFEPILGEKKAKELLTGSHTRTVTMASASSSTGGLMKFVKKVETCKGCKNPLPKNNLGALCDNCKPKAGKLYSEALAGMNALQVKFSRLWTECQRCQGSLHQDVLCSNKDCPIFYMRKKAQKDVIAQSKELQKWNDTFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P097</accession>
    <name>A0A1E3P097_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_31535</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. MetX family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037982.1">
        <property type="nucleotide sequence ID" value="XM_019181845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P097"/>
    <dbReference type="STRING" id="683960.A0A1E3P097"/>
    <dbReference type="GeneID" id="30199091"/>
    <dbReference type="OrthoDB" id="444135at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004414">
        <property type="term" value="F:homoserine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009001">
        <property type="term" value="F:serine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009092">
        <property type="term" value="P:homoserine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00296">
        <property type="entry name" value="MetX_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01392">
        <property type="entry name" value="homoserO_Ac_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001209">
        <property type="entry name" value="PRK00175.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268:SF16">
        <property type="entry name" value="SERINE O-SUCCINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000443">
        <property type="entry name" value="Homoser_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="114"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ58775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="498" mass="56095" checksum="D6F549F5DBD822B6" modified="2017-01-18" version="1">MQKNTYSTKTSKRRIHHHNQDHPARPSNPAMNFPCLDRLQAKTDLLNSGQTPTNSSTNTEIKTNQGPEPVYSRVQSGFKKYHSDSPLFLDYGGFLPEYEIAYEQWGTLNPNKSNAILLHTGLSASSHAKSQVENTKPGWWEKFIGPGLPLDTDKYFVVCTNVIGGCYGSTGPSSIDPSDGKHYATRFPILSVNDMVRAQERLMREQFGVEKLYASVGSSMGGMQSLAYAQEFPEKVEKIVSISGCARSHPYSIAMRHAQRQVLMSDPNWKRGFYYDSIPPHVGMKLSREIATVTYRSGPEWEIRFGRERADPSQKPALCPDFLVETYLDHQGNKFSLEYDPNSLLYISKAMDLFDLGKVNRDLASSKRQEAFERYETEEYEEFAASCASSSVPDGEYKVKPKTRTITKQESDDDLLHGLKTFANKQVLVVGVKSDILFPHWQQLEIANMLKNGNKQFFGGDGSNVQHVELGEDISMYGHDTFLLDVEHVGGPVKRFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0N3</accession>
    <name>A0A1E3P0N3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA(adenine(34)) deaminase</fullName>
            <ecNumber evidence="3">3.5.4.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_32383</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>adenosine(34) in tRNA + H2O + H(+) = inosine(34) in tRNA + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:43168"/>
            <dbReference type="Rhea" id="RHEA-COMP:10373"/>
            <dbReference type="Rhea" id="RHEA-COMP:10374"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:82852"/>
            <dbReference type="EC" id="3.5.4.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family. ADAT2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.33" evidence="3"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037971.1">
        <property type="nucleotide sequence ID" value="XM_019181950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0N3"/>
    <dbReference type="STRING" id="683960.A0A1E3P0N3"/>
    <dbReference type="GeneID" id="30199196"/>
    <dbReference type="OrthoDB" id="1701769at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052718">
        <property type="term" value="C:tRNA-specific adenosine-34 deaminase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000039">
        <property type="entry name" value="tRNA-specific adenosine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF149">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="9">
        <location>
            <begin position="5"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ58764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="251" mass="28976" checksum="68F9DE671C59B506" modified="2017-01-18" version="1">MVELEKHYEFMRYALRLANNALHTNEVPVACVFVYDGQIVSYGSNNTNDSLSGITHAEFRGINIILDKVKSSPDFQQVYQNPQDIFKDIDLYVTVEPCVMCASALKQIGIRSVFFGCGNERFGGNGSVLRINKDCTTPENNYNAFPGFYRREAILLLRDFYTHENTHAPVPKSKKNRNLNKETYPDLIWSNYLNKDEFISMFGEDKIEIFEENRDLIEEVDESVLEPNNIDISDIIKFTETPLSSFKRRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0P5</accession>
    <name>A0A1E3P0P5_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_93939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038256.1">
        <property type="nucleotide sequence ID" value="XM_019186433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0P5"/>
    <dbReference type="SMR" id="A0A1E3P0P5"/>
    <dbReference type="GeneID" id="30203679"/>
    <dbReference type="OrthoDB" id="10662226at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="285" mass="32852" checksum="C95DDB6790B5D582" modified="2017-01-18" version="1" fragment="single">MAPPSIRPPGARSMQSLNSVASSNYSQGGPNPHYQSQQHIPQMLPQGEWASGRMSPPKSPSLRLNSISSNSMQFHAPQQEYKNISPVLSQSEWDRPFQDERIKELELENEKLLDENEELKQNVEFKTRDITSDNDRLKTQLSTVQMELNKLKLEHNSLKETHKSEVKKLKERNSELQNDLESMSYMNERSNETTPNGSPKTLGKEYESLNPANTTTLPESSKHLQQAQSNLENNELVQDLLETIKYLKTDNHILRNQNDDLVTRMIKDKKYMQKLVNSSRYQTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0P7</accession>
    <name>A0A1E3P0P7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_84684</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038218.1">
        <property type="nucleotide sequence ID" value="XM_019185754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0P7"/>
    <dbReference type="STRING" id="683960.A0A1E3P0P7"/>
    <dbReference type="GeneID" id="30203000"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000386">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000065">
        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF39">
        <property type="entry name" value="TRANSPORTER LIZ1_SEO1, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G00980)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="163"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="435"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="469"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="512" mass="59075" checksum="D8A22DD54306D745" modified="2017-01-18" version="1">MSTTPNKQEVFNDIQSVSSTDSKNNHRSKVVELTEEELKRRNTWYYKWSLFLWDSVDKHPLERSFLFKLDFFLLSSSMLGYFIKNLNQSNVGTAYVNGMKEYYHMNGNQYNYMQTLWTVGYIIGQIPSNLILHRISARYYLAGLELIWAFLTVLLIVPKSLNGFYAIRFFIGLTESGYFPGLEYLIGSWYSRKELTKRSTFFACAGTAAGLISGPLQQSILDSKWAQKGLHPFQWMFVIDAVISAPVAFYTLFTDPNTPSTTTAFYFTETDKKVALERRRRIGAQLNTRESYSFAKIKSFFNTWHVWVLPFLFLTFNNSYTPISQQAFQLWMKNGLKLKSYQYNIYPTAISAGGIGFSILVAYTNDFFRGRLNPYFLIFMFTSVIFSCACLSVWNIPIGLHWFSYFGIGIPLSVGQPLIFSWVNRMLAHDDMKRNFVVVCTNTLAYVTGAFVPIFTFNQTQNPEFHIGFTYTTCLSSLGLVLTVVTLFLSLRDDKRRKQSFEEKGEELPSWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0W3</accession>
    <name>A0A1E3P0W3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_105447</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037769.1">
        <property type="nucleotide sequence ID" value="XM_019180446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0W3"/>
    <dbReference type="STRING" id="683960.A0A1E3P0W3"/>
    <dbReference type="GeneID" id="30197692"/>
    <dbReference type="OrthoDB" id="2962993at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000013">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000034">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF18">
        <property type="entry name" value="NICOTINIC ACID TRANSPORTER TNA1, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G03820)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="163"/>
        </location>
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            <end position="194"/>
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        <location>
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            <end position="414"/>
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        <location>
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        <location>
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            <end position="474"/>
        </location>
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        <location>
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            <end position="481"/>
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        <source>
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        </source>
    </evidence>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="494" mass="54984" checksum="626748EC8BB0C8EE" modified="2017-01-18" version="1">MVEIDKSETQFIEDINTSNNSSNESPNNDFSDLNETKLLRKMDLHILPVISVLYLLAYLDRGNIGNAKIEGLPESLNLSSSEYNICLTVFFLTYASFEVPSNMLLKKIGKQSVFLPTIMLCWGIVMVCMGVVQNYAGLLITRLLLGLFEASLYPSIVFCLTQYYCKREMQFRQAILHGSASAAGRFSGILAFAIAKMNGVGGYEGWRWIFILEGLLTVIVAISAYFLLYDYPDTAKFLTEREREFIIWRLKNDSNPSKNLNDLDSFSSREKPDFQKFSEADDLSLKKSLVAALKSYEVYLYVLTYYGVIVPTYGFALFLPSVVKELGYTSSTAQLMTVPIYVTASIVSIIVAYYSDKTGLRSPFIVGSLFLVMLGYIFALVGEEIGYPNLIYGGVYIAGIGVYSAFPGAVSWLVNNLANSRKRAIGLALQLGIGNFGGAFASNFYIPGKYSMGHGLEIGFSSMGIIVTGIIVLYNQRCNRKRQRDLANGKYDEI</sequence>
</entry>
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    <accession>A0A1E3P0Y8</accession>
    <name>A0A1E3P0Y8_WICAA</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">WICANDRAFT_63610</name>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <evidence type="ECO:0000313" key="1">
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            <dbReference type="EMBL" id="ODQ59111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="228" mass="26758" checksum="03196BBA899E65B4" modified="2017-01-18" version="1">MPSLPSFKINYSVSLNGELLETSSFITEYLMKSMESSKFWNQLHFKLETNYSKSVNHSYICQIHDELINQSILTKFGNENFINLEYITIELEFYTSDLKLDTMIELINYFHYSLLFTALEFEMPFVFNYYSRKCFGDNFKYLVSISNSLTKTNPNKLIQSLLNLIHNYPQSSAKYLYELSTHSYKRNYQSQKFKKLKGEIDHTLTLFNDSSIDLKQISQLTNGCPSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P173</accession>
    <name>A0A1E3P173_WICAA</name>
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            <fullName evidence="2">Phosducin domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="3" type="ORF">WICANDRAFT_63730</name>
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    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
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        <name type="synonym">Hansenula anomala</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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            <taxon>Wickerhamomyces</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the phosducin family.</text>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071444">
        <property type="term" value="P:cellular response to pheromone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903333">
        <property type="term" value="P:negative regulation of protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="Phd_like_VIAF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosducin-like_chap/apop_reg"/>
    </dbReference>
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        <property type="entry name" value="Phosducin_thioredoxin-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45809">
        <property type="entry name" value="VIRAL IAP-ASSOCIATED FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45809:SF3">
        <property type="entry name" value="VIRAL IAP-ASSOCIATED FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02114">
        <property type="entry name" value="Phosducin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosducin" evidence="2">
        <location>
            <begin position="34"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ59236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="251" mass="28686" checksum="5A187DF850D9E427" modified="2017-01-18" version="1">MDPKINVQVDPNEDTEWNDILRKHGVIPERPPSPTAQLEEALQEAVVKQYENRLDGKDLDELEALEDEEDEDFLEEYKQKRLKELAQLNQVSKFGSVYPVAKPDYKSEITDASKDTLVWTHLTLQSSLQSRLLSSLLITLASKFKEIKFVEIPGSRAIENYPDANCPTILIYFNGDVIKQYITLTQLGGNSTNIKDLENILVEIGAVKDSDKRLAINQDDEDLEEAHRLRFAKKSIRGKLDDDDDDDDFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1L8</accession>
    <name>A0A1E3P1L8_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_32790</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038586.1">
        <property type="nucleotide sequence ID" value="XM_019182005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1L8"/>
    <dbReference type="STRING" id="683960.A0A1E3P1L8"/>
    <dbReference type="GeneID" id="30199251"/>
    <dbReference type="OrthoDB" id="4063222at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="169" mass="19532" checksum="2C66395890B7ABB9" modified="2017-01-18" version="1">MGQLLNLKKVKSDSQNDFLVNSLETITSLMDNVYLITKFGIGQNSKFLSKIGSNASKVWFVTLLITIRKIIKDLIRLYNLKTQVWKEIKFCEMEQLHNPLAKLILEKYELKLQDIQNDILGGALELVGSLNDLFFVSIEIFKLKVPRWIEKLIGFISAAMMIYRMSKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1R1</accession>
    <name>A0A1E3P1R1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_63352</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFB1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038054.1">
        <property type="nucleotide sequence ID" value="XM_019183531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1R1"/>
    <dbReference type="STRING" id="683960.A0A1E3P1R1"/>
    <dbReference type="GeneID" id="30200777"/>
    <dbReference type="OrthoDB" id="360521at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13229">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027079">
        <property type="entry name" value="Tfb1/GTF2H1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013876">
        <property type="entry name" value="TFIIH_BTF_p62_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12856">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08567">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BSD" evidence="8">
        <location>
            <begin position="132"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="BSD" evidence="8">
        <location>
            <begin position="207"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="359"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="586" mass="66692" checksum="63F417FE5C18DB1C" modified="2017-01-18" version="1">MSLVSGAALYKKKSGLLTIYEDRSPAILLWKAADNALPIPPVEIVLTTITNMQATPASSPKMMLKIFTKPSEGAEPVVYIFSFTNRIVMDNVKEALQQIGARHKSALQAAQSANSSPAPEKPLINTTFLDDALDSKKLLRNHQLQQKLLLENKPLMSTFKEAVMNQGLDPEEFWTTRVHLLRAFALSHSQKIGPYNVLSTIKPTATSDNQVNVNVSRDKIHAIFEQYPVVRKAYDDNVPRLSEGEFWSRFFASKLFRKLRGEKINPNARGDLLLDKYINMDIDYEKHEDETLNHKVNKTIDLEGNQEDDSTKLGNAPDMTMKANAMPETVSVLRSMNRLSQKMVNSLEHEYSRSATPGENEKSKEERENERIRDELLFHDLEVPHNTEYSEISVRQDRETHEINRPKPPTRQEYAQYVDELKTQFEQPADLETIFSNDRKRPIEEASADVINTVRINSKQSKQSWQVYRSFEEQAAHSVVDEADETFDKNALEQLRITHGTSVEFLRHFWLHFSSGDPSQATNIKTLYNSLVKSQDRVKAALESIKDEKVKEQSSHYMKPLNESLTAAIAKYQNALKEAGVTITSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1Z0</accession>
    <name>A0A1E3P1Z0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_79867</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLA2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038555.1">
        <property type="nucleotide sequence ID" value="XM_019184966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1Z0"/>
    <dbReference type="STRING" id="683960.A0A1E3P1Z0"/>
    <dbReference type="GeneID" id="30202212"/>
    <dbReference type="OrthoDB" id="10262320at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032051">
        <property type="term" value="F:clathrin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043325">
        <property type="term" value="F:phosphatidylinositol-3,4-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17007">
        <property type="entry name" value="ANTH_N_Sla2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000012">
        <property type="entry name" value="Huntingtin interacting protein 1-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035964">
        <property type="entry name" value="I/LWEQ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002558">
        <property type="entry name" value="ILWEQ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030224">
        <property type="entry name" value="Sla2_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10407">
        <property type="entry name" value="HUNTINGTIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10407:SF15">
        <property type="entry name" value="HUNTINGTIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01608">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00307">
        <property type="entry name" value="ILWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109885">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50945">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="7">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="I/LWEQ" evidence="8">
        <location>
            <begin position="794"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="256"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="320"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="447"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="275"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1043" mass="117282" checksum="03A3676B882D483A" modified="2017-01-18" version="1">MSKSEADLQTSIRKACSTDETAPKRKHVRACIVYTWDHKSAKAFWNSIKIQPIQSDEVQLFKALITIHKVLQEGHPSALKDGIRNTDWIESLGRSIHGDGFKGYGRLIKEYTLYLLRKLHFHRNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMALQDALDDFQRLIFASISHDRKSECKISALVPLVAESYGIYKFITSMLRAIYRSTGSDDALQPLRERYDSQHSRLFEFYADCSSIRYLTSLITIPRLPESPPNLFVEDDDTDDRPVSRQASQPTTSAPSPEPISAQPTTGQFQQPIPTQPTGVDFWTNQQSNYEAEQQRLAREQQQQALQQQQYAEQQRQLFEQQQQQQAEQQRAAQEQLYREQLQSQAQGRVAELERDLLALRGQYERDQLLLSQYDQRVQALETEIANTTQSATQQLASKDELTNSLQEQMGIWKSKYESLAKLYSQLRQEHLNLLNKFKKLQQKAASAQEAIDRREKLEKDLKAKNVELAGLIRERDRARLDLEKIKGGKDSEIDKLELQVRDLTLKLDDSERSQSSNLSSIFAQHKKEIESLKSQLEQQSRSFNPDLERQLREKDEELEIVQQTMDDALQELARVQQENDTAIDEQIDEVLSDHLNKLVSLVDAILQSGIKRIQDSVYELDSPMQAGNQNSSPEYLLSVIEKASSSATDFANSFNDFIADGPNGDHSEIIKSINDFSSAISDVLLNAKGIVRLVKTDAEADKIVISSRDTAQESESFFKSLLSTNLKGTEEEKTETVITGNLNVQEKLQYLINLAEGLAPRSVITHTAGDLGEVVDNELNSAANAIASASSHLSSLLNKPQDPSLSQIDFEINKAILSAAIAVTNAIALLIKAAIATQDEIVSQNKGSQTRAQYYKKHNRWTEGLISAAKAVAGSTKILIETADGVLSSKNSHEQLIVASNEVAASTAQLVAASRVKATFMSKTQDQLEVASKTVTSACRELVNQVQDILSKRTNGSADEVDYSKLSNHENKTVEMEQQVEILKLENALGAARKRLGEIRKYSYRDGDSDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P223</accession>
    <name>A0A1E3P223_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_30487</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038746.1">
        <property type="nucleotide sequence ID" value="XM_019181737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P223"/>
    <dbReference type="STRING" id="683960.A0A1E3P223"/>
    <dbReference type="GeneID" id="30198983"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19071">
        <property type="entry name" value="AKR_AKR1-5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF13">
        <property type="entry name" value="ALDO_KETO REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="27"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="4">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="273" mass="30978" checksum="375E4A5CD80428F6" modified="2017-01-18" version="1">MSKLTPTSTLTLNNGYKIPITGLGVFQTDVQETYDIVYKALEAGYKHIDSAIYYKNEKEVGDAILQFLKDHPEFKRSDIFYTTKVAFAADYETAKKAIHDSFEKVKGLGYIDLFLNHSSLPDKTGRLGTWKALQEAVDEGIVRSIGVSNFGTHHLDELYGWEGLKIKPVVNQVELHPWLPRKDLQEYAKKYDYILEAYSPLTQAKKLDAPELVSIAKKHNVSTAQVLLRWSYDQGFLPLPKTVTPKRLLPNLTVLDDVELDQEDLAKLNFPDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2F5</accession>
    <name>A0A1E3P2F5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">60S ribosomal protein L11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_78288</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038858.1">
        <property type="nucleotide sequence ID" value="XM_019184698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2F5"/>
    <dbReference type="STRING" id="683960.A0A1E3P2F5"/>
    <dbReference type="GeneID" id="30201944"/>
    <dbReference type="OrthoDB" id="1734943at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1440.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020929">
        <property type="entry name" value="Ribosomal_uL5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057266">
        <property type="entry name" value="Ribosomal_uL5_euk/arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_uL5_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003258">
        <property type="entry name" value="PRK04219.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00358">
        <property type="entry name" value="RIBOSOMAL_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL5 N-terminal" evidence="6">
        <location>
            <begin position="7"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL5 C-terminal" evidence="7">
        <location>
            <begin position="64"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="19827" checksum="090F12F535B9B646" modified="2017-01-18" version="1">MSAKAQNPMRELKIEKLVLNISVGESGDRLTRASKVLEQLSGQTPVQSKARYTVRTFGIRRNEKIAVHVTVRGPKAEEILERGLKVKEYQLRNKNFSQTGNFGFGIDEHIDLGIKYDPAIGIYGMDFYVVMGRPGNRITRRKHAKAVVGNHHKTTKEDTVQWFKQKYDAEVLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2S2</accession>
    <name>A0A1E3P2S2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_62229</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038850.1">
        <property type="nucleotide sequence ID" value="XM_019183334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2S2"/>
    <dbReference type="GeneID" id="30200580"/>
    <dbReference type="OrthoDB" id="3987248at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070336">
        <property type="term" value="F:flap-structured DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000736">
        <property type="term" value="P:double-strand break repair via single-strand annealing, removal of nonhomologous ends"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021624">
        <property type="entry name" value="Saw1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11561">
        <property type="entry name" value="Saw1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ59643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="222" mass="25896" checksum="892867936FEA35F8" modified="2017-01-18" version="1">MAPITTFIKISPNTTIPLRIFINRKSIIQQQQQQYFETAISSAQTVNHRALVRLRSTTRLILSNRQIRSLVASLQAELCMILFETSPESLIMKNEGDGKERLKFKVDVDGWELYVNLVMGDVMEVRNMMKFGEVDKKEYNGMELLIRKPREVKQREEQEEVSLLQEGEDEEMRIPEEGEVVRIPDEEDSTEDKKTQLKYDYKGTNILLKRGINIEVLKIPKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2T1</accession>
    <name>A0A1E3P2T1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_30242</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038998.1">
        <property type="nucleotide sequence ID" value="XM_019181705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2T1"/>
    <dbReference type="STRING" id="683960.A0A1E3P2T1"/>
    <dbReference type="GeneID" id="30198951"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045937">
        <property type="term" value="P:positive regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120213">
        <property type="term" value="P:regulation of histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006141">
        <property type="term" value="P:regulation of purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051000">
        <property type="entry name" value="Homeobox_DNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324:SF5">
        <property type="entry name" value="HEMATOPOIETICALLY-EXPRESSED HOMEOBOX PROTEIN HHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324">
        <property type="entry name" value="HOMEOBOX PROTEIN HHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="21"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="23"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="391" mass="44308" checksum="1B42F972F6B7F761" modified="2017-01-18" version="1">MYNEFQDSTNNNDGSQQQGGNEGAQKRTRASGEVLALLVNEFNKNANPNAQTRKRISEQTGMPERSVRIWFQNRRAKSRKMEKLQANEKEDPYTFGVGSYVSSTTRYDNIPLTLNNNYFFIDAKSLTVGSWKRIKSGNLSNDNVFSVKNLSNLSPFSINEIMSNSTDLMIVISRKNFEINYFFSAITDTSKILFRIFYPISQVNNCSISISQDGDDTNSSLCELKLLLNKPPKFAVYFSQTMAQNVNQWSICDDFSEGHQVNDAFIGGSGLPHVFTGLEQSLKFMNSFILDYNSTNVSQQEFSLPQRFDSRSPTIINNYPNHQPPQQIQENNDNDIADFEFNSNPLLQQFVQDPNFSNDPNDAFENFIKEDQGTPSGPSFDLTGAENNIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2Y2</accession>
    <name>A0A1E3P2Y2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Translocon Sec61/SecY plug domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_78478</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039053.1">
        <property type="nucleotide sequence ID" value="XM_019184730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2Y2"/>
    <dbReference type="SMR" id="A0A1E3P2Y2"/>
    <dbReference type="STRING" id="683960.A0A1E3P2Y2"/>
    <dbReference type="GeneID" id="30201976"/>
    <dbReference type="OrthoDB" id="420669at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005784">
        <property type="term" value="C:Sec61 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005048">
        <property type="term" value="F:signal sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070843">
        <property type="term" value="P:misfolded protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3370.10:FF:000002">
        <property type="entry name" value="Transport Sec61 subunit alpha isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
        <property type="entry name" value="Translocon_Sec61/SecY_plug_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006341">
        <property type="entry name" value="PRK08568.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00756">
        <property type="entry name" value="SECY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Translocon Sec61/SecY plug" evidence="13">
        <location>
            <begin position="41"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF10559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ59846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="479" mass="52972" checksum="9B086AA0E89E46BE" modified="2017-01-18" version="1">MSSFRVLDLVKPLTGYLPEVIAPERKVPFNQKLMWTGVTLLIFLVMSEIPLYGIVSSESSDPLFWLRMMLASNRGTLMELGISPIVSSGMVFQLLQGTQLLDVNLESKTDRETFQTAQKLFAILLSIGQATVYVLTGIYGRPSDLGVGVCLLLILQLVFAGIIVILLDELLQKGYGLGSGISLFMATNICEQIFWKAFAPTTVNNGRGDEFEGAVVALFHLLSVRKDKRRALVEAFYRQNLPNIFQLLATFIVFFLVVYLQGFRYEIPVRSTRQRGQNGLYPIKLFYTSNTPIMLQSALTSNFFIISQMLFQRFPLNPVVRLFGVWDARPGSAQLFASNGLAYYIQPPLSLTEALLDPIKTVIYVSFVLSVCALFSKTWIEISGTAPRDVAKQFKDQGLVIAGRRETSVYKELKRIIPTAAAFGGASIGALSVACDLLGTLGSGTSILLAVTTIYSYYEIAAKEGGFQKHIVEGFSEAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P301</accession>
    <name>A0A1E3P301_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_22558</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039066.1">
        <property type="nucleotide sequence ID" value="XM_019180953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P301"/>
    <dbReference type="STRING" id="683960.A0A1E3P301"/>
    <dbReference type="GeneID" id="30198199"/>
    <dbReference type="OrthoDB" id="68483at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14008">
        <property type="entry name" value="STKc_LKB1_CaMKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF72">
        <property type="entry name" value="SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="4"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="274" mass="31056" checksum="7BFBC0E4A5317108" modified="2017-01-18" version="1" fragment="single">LNQYKLISKLGNGHNGKVYLGLNVDTGGYYAIKEISKVSKISILNKDPQNQLTKINNEINIMKLIINHPKILKLHEILNDLKFNKIFLILEYCSKGDLKFGATFNYSIDSIRLILRDVVLGLEYLNGVGIIHRDIKPSNLLMNSDNFVKISDFGISIDKFRNSNINNFGTPAFTPPELCNGVENGNGYNKFDGKLDIWSLGVTIYCLYYQVLPFNGNNEYELFNEIINNEVVIPSDGDGDEQVGLLNDLLIKMLQKNPQKRISLQDLKNHPFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P342</accession>
    <name>A0A1E3P342_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide exchange factor LTE1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_83922</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the LTE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039113.1">
        <property type="nucleotide sequence ID" value="XM_019185620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P342"/>
    <dbReference type="STRING" id="683960.A0A1E3P342"/>
    <dbReference type="GeneID" id="30202866"/>
    <dbReference type="OrthoDB" id="10254377at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000019">
        <property type="entry name" value="Guanine nucleotide exchange factor LTE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF363">
        <property type="entry name" value="PROTEIN SON OF SEVENLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GEF" evidence="11">
        <location>
            <begin position="192"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ59906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="438" mass="50086" checksum="2AC9742EBBE61F95" modified="2017-01-18" version="1">MSTNSIFSDAKRNQLVMVHEEHSVNEGDKLVLRADLDRAKQKLIQQDQDYESSIKLVESSDFAIPYPGVDGDAIAELAAISDDSFQSDPINAALLKLEGTYTINKGSNRSIDSKELQRQVDDLDIKSARNSVFVDQRRKTKLFSLTPVKQKPISIQSSSKILLELLINHKITNEVLSIGNNANHISFILNFDSKTLAQQFTLVEKDCLLEIDWKELIELKWNFNNIVSINSWLELLIQNETIKGIDLCISRFNLTVNWIISEILLTKDITLRKLTIQRFIHIAQNCMMLQNYSTLMEILLALGSDKILKLKNTWRIIEPGDILIYKNLEKISSPFKNFANLRNELNNLKPSKGCVPFLGLHLSDLIFNKEKNSTKNDLINFNKFRTDSKIVKSLIQCIQWSTLYRLDSADEVLSKCLYIKALDEEEMNECLKDIEGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P397</accession>
    <name>A0A1E3P397_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_56036</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038579.1">
        <property type="nucleotide sequence ID" value="XM_019182768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P397"/>
    <dbReference type="STRING" id="683960.A0A1E3P397"/>
    <dbReference type="GeneID" id="30200014"/>
    <dbReference type="OrthoDB" id="166134at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098588">
        <property type="term" value="C:bounding membrane of organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15761">
        <property type="entry name" value="FYVE1_Vac1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15737">
        <property type="entry name" value="FYVE2_Vac1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052727">
        <property type="entry name" value="Rab4/Rab5_effector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021565">
        <property type="entry name" value="Rbsn_Rab-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036531">
        <property type="entry name" value="Rbsn_Rab-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13510">
        <property type="entry name" value="FYVE-FINGER-CONTAINING RAB5 EFFECTOR PROTEIN RABENOSYN-5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13510:SF44">
        <property type="entry name" value="RABENOSYN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11464">
        <property type="entry name" value="Rbsn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140125">
        <property type="entry name" value="Rabenosyn-5 Rab-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="326"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="274"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="599" mass="68548" checksum="39B0F963FBA67388" modified="2017-01-18" version="1">MSSDNPTTSITKAPTRRVLGAAPKRILGVPKSPSHSKQSSNGSINTPTPSPLKIERRDSPALEDHITPVKAPAPVFINGGANTPDILLSEADVIDGVSSPDPFIERNLTSPQPDAKSSDENLDEITCPICDEKMISLLQLNRHLDDEHKFQDAFESSSSSLNTVTPEITVTRKHWQKPSGYDRCFVKGCKKHLNIKNGLVNCRKCGKLFCHDHSDFRVKLDNNADYYAEGVWCKCCEDCFKYKPGYNDYGLVNNLTEQFKQKRQSRIDEKQLYANKLEKRIINLTKTLSQIDKEFGSTDFNSNFLNYRIINKKREAEQQLVQWEDEKLVLNCFLCLRNFSFTLRKHHCRLCGRVVCGSETTGCSKEIPVNILTELLQDLPKTNDDSLLRLCRNCKNVLFLKRNFQKDLRKPFSKLLAKFEIQQNVKKSITTLMPRFQDMLIKLQDDKAVRSKSIIADASKLRKRLMDTFAMFDKLTKEIVTLECSTESESRIQLAIQTESASFIQKNMLPLKSLPKILKQQELKQKEQHKVLSREEVQGIKQNREELMVLNEQKFLVEDMIANCKAQRKFDELPPLNENLDELNKNIDRLQAALGNEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3C3</accession>
    <name>A0A1E3P3C3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_62289</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038911.1">
        <property type="nucleotide sequence ID" value="XM_019183341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3C3"/>
    <dbReference type="STRING" id="683960.A0A1E3P3C3"/>
    <dbReference type="GeneID" id="30200587"/>
    <dbReference type="OrthoDB" id="9974421at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004771">
        <property type="term" value="F:sterol ester esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000095">
        <property type="entry name" value="Triglyceride lipase-cholesterol esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="9">
        <location>
            <begin position="127"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ59704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="462" mass="53228" checksum="1EDA7588DE5C5F92" modified="2017-01-18" version="1">MVIPFLGRLSLLEWPMVIVSFVFAYIEFIISIITKCLPSPIIHLCTNIINQVYTVFLLSPNEEDIYSIPKTKSYIDMLKLRNKIQNTENIKQIGEIFGHEIDDHIVKTNDGYLLCLHRIKPKVENAPIVYLHHGLLMCSDIWCVNIEKNKNIPFLLHDLGFDVWLGNNRGNKYSTKHIYLKPNDVKFWDFSIDEFAIYDIPDSISYILNYTNQQDLTYIGFSQGSAQAFAALSINPILNKQVKLFIALSPAMTPRGLHHKVVDALIKTSPNIMYLIFGKKTLLPTASFWESIIYPPLFVKIIDKSNEMLFNWKSLNINFNQKISSYSHLYSPTSVKSIVHWFQIIRNSNFQMFDEDFSITNTISKPFRNPNFPTRTNIKVPIRLIYGTIDSLVDINVMKKLLPSDDIETVGVLDHEHLDLIWGDDIEDLVFPHILKFLNIDSKGTSAIQNDNDSDNEKVSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3D7</accession>
    <name>A0A1E3P3D7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_68435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039070.1">
        <property type="nucleotide sequence ID" value="XM_019184118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3D7"/>
    <dbReference type="GeneID" id="30201364"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="387" mass="45981" checksum="5CC277D331CA4929" modified="2017-01-18" version="1">MAYQSRYLIQENINNFVYIKLIGYNTSSNPLVSCNTENIGIDTGEYNLQTKPTSNNKPSGTRTPLAPINPNGANTTVTNTTPTPDYDVYGMESLKWSLAMVGEFAELVVEYQHYIIGNYNEFNALGWEIIQSRMWDSFPDFHDFLKIGDLVKQLRYLCGQYLLYYQILRDKCYDVFQYSEFHNHFILDKDVYDIFPREFFERSKKNKYAFRFLNYEKELQKCGRNMLDDIIDIVGRAEYENRKHNLVEYFTRDISYMVLLEEFVNEPSVYLWKNRKARAKHNYNLEERKIFKGKHKLSKKATARFEKNKKHIRENYEKYLTFLKKIPKRLSPTELELNKLLMTIEKESALVNIIAKDEELDTRQKIFVIKHCLGRSDKSIEWDMTIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3M6</accession>
    <name>A0A1E3P3M6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_83924</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039115.1">
        <property type="nucleotide sequence ID" value="XM_019185622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3M6"/>
    <dbReference type="GeneID" id="30202868"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133640" description="Secreted protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="16142" checksum="F4A95A427F722DB2" modified="2017-01-18" version="1">MQFKNLLLSVTALASIVAAAPIAAAEGSDSANSPVGFVATSGSDPTFYLQFKEGTISGPAKVDVEGEVYSTVNNKLTIKNFSASKVSGDEISKTHSTTAFSGKWHVDNNDDTFSFLVSTANSGSSTSGSKTVFKGSYTLDSDGEQHNFTVPVNAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3Q2</accession>
    <name>A0A1E3P3Q2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_78738</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039328.1">
        <property type="nucleotide sequence ID" value="XM_019184780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3Q2"/>
    <dbReference type="GeneID" id="30202026"/>
    <dbReference type="OrthoDB" id="3982901at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="339" mass="38937" checksum="89CBE490D63E2C34" modified="2017-01-18" version="1">MSYSDFSFNSSPTIEEIRHSLAHTLHFVRRDDYSANQSLLDQCSFKLDQASTQLDYDCMEKSVIAVLMNVPVLPTNNSKKNSQHTFKETGTLYNENILLFHAYIREIAVSIMFVKGCPLKTGRLSEILSRDELADLVKFIAWNFKPLFYIVLDSTPFLLNNSRLVIEATLQKYFLDISRHLSRTHLQKLFQKIVPQKFQIKPYPSTIIQSHLVKLIKSHGIESPFIGTSTGITFARGIDFKKCNYIYIEQPGGFPHRPRDELKTLHLKDIYPNLSQVDLIHDDIVKLLTKLDIVPFKRINTNQIVKPVILPSNSAGPYEDDFKFIAELTELQMINDVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3U5</accession>
    <name>A0A1E3P3U5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Gfo/Idh/MocA-like oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_31493</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039373.1">
        <property type="nucleotide sequence ID" value="XM_019181840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3U5"/>
    <dbReference type="STRING" id="683960.A0A1E3P3U5"/>
    <dbReference type="GeneID" id="30199086"/>
    <dbReference type="OrthoDB" id="64915at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051450">
        <property type="entry name" value="Gfo/Idh/MocA_Oxidoreductases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377:SF1">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="11"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase C-terminal" evidence="2">
        <location>
            <begin position="140"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="41162" checksum="EA310C8377FF0DAD" modified="2017-01-18" version="1">MPSKNSSSPLKLIVVGLGLIGPRHAEQIQLNPDAELIGFVDPSPKAGVIAKRFKVPLYESLLQLLDSGLKPDAAYVCTPNSLHIKVAMELASNGIHMLIEKPLSTNLQEAVLLKNYAVSKGVKILVGHHRRFNPYILIAKENLERLGQVVAIDGVWTLKKNDTYFKQVEWRNLKSQGGGAIMINLVHDLDLLQYLLGPITKVYADYLPKNRAAASTADQVDEGCVCTLSFQSGAKGTFIVSDNVISPFNFEMGTGENPIVPKVESTTDGIFYRIFGTNGTMTIPNLKLYNQGYLENEGKEKGWYEIIQCEELSIGEDIEGIPFALQLSHFLKMMKDEEAPKCTVDDGISVLLVVEAIVESLETGPQTVKSVDSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3X2</accession>
    <name>A0A1E3P3X2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_30761</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039403.1">
        <property type="nucleotide sequence ID" value="XM_019181758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3X2"/>
    <dbReference type="STRING" id="683960.A0A1E3P3X2"/>
    <dbReference type="GeneID" id="30199004"/>
    <dbReference type="OrthoDB" id="189226at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036149">
        <property type="term" value="P:phosphatidylinositol acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07990">
        <property type="entry name" value="LPLAT_LCLAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032098">
        <property type="entry name" value="Acyltransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF16">
        <property type="entry name" value="LYSOCARDIOLIPIN ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16076">
        <property type="entry name" value="Acyltransf_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="5">
        <location>
            <begin position="100"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="404" mass="46099" checksum="96A5E8CD7E7F10A1" modified="2017-01-18" version="1">MTALGVVKTVLNLVVFLLGCLSIVTSQTVGIILSCGNREFRQAWLAQTKEHFIVLLTSVVNFASKSPIKVRIVTDSKSIPPSRIRLDSKGRLESSMPGGTVFIANHQIYTDWVFLWWLTYTARLGGFVYIVLKASLKKLPILGYGMKNYDFIFLSRKWETDKVVMGNQLASIDANARGKGTVAGKEPMKTTTSKDVWPAGEDKSRTWPYSLHIFPEGTNLSANTRRKTLAYAGKVGRAPFKHLLLPRTTGLRYSLLKLRDTVDEVYDITLAYSGLKASDYGQDIYKIEKVFLQGKNPERVDFFIRSFKIDEIPIGKEEETEDEYNIAQKKFEDWLFNVWSEKDELMDNYYKTGSFITNAEEPDRYSTVDTVLRVSKLDFLKIFLVPMCLVLLGRIIYQLYCLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3Z0</accession>
    <name>A0A1E3P3Z0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">V-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_91322</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038804.1">
        <property type="nucleotide sequence ID" value="XM_019186285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3Z0"/>
    <dbReference type="STRING" id="683960.A0A1E3P3Z0"/>
    <dbReference type="GeneID" id="30203531"/>
    <dbReference type="OrthoDB" id="190375at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15874">
        <property type="entry name" value="R-SNARE_Snc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016444">
        <property type="entry name" value="Synaptobrevin/VAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45701">
        <property type="entry name" value="SYNAPTOBREVIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005409">
        <property type="entry name" value="Synaptobrevin_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="25"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ59597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="113" mass="12623" checksum="B5AECA3C065979CD" modified="2017-01-18" version="1">MSSSVPYDPYIPSGESNNQTQSNSRTAALQSEIDDTVGIMRENIDKVARRGDNLSSIENKADDLAVSAQGFRRGANRVRKQMWLKDMKMRFCLVAVVIILLIVIIVPIVVHFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4A0</accession>
    <name>A0A1E3P4A0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Histone-binding protein RBBP4-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_84204</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039526.1">
        <property type="nucleotide sequence ID" value="XM_019185663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4A0"/>
    <dbReference type="STRING" id="683960.A0A1E3P4A0"/>
    <dbReference type="GeneID" id="30202909"/>
    <dbReference type="OrthoDB" id="2161379at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051972">
        <property type="entry name" value="Glutamate-rich_WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903:SF1">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="7">
        <location>
            <begin position="99"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="309"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="403"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="34"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ60319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="501" mass="56031" checksum="EA51C5BD42EB1B2C" modified="2017-01-18" version="1">MSKRLNEDVEDIPRNTASKGAAGDIPNANKIEALENDVDMGEFEDPYSDEFESDEEIVEIDSNGEGEEDAQDVIQKDQGEDLKESTIYLPHKSKPLGPDEVLEADPSVYDMLHNVNLPWPCLTLDILPDHLGNERRNFPASLYVTTATQAEKKKDNELYVLKLSQLSKTLVKDEEEDADDENEDEEYEGDPIMESESIPLRDTTNRIRVSPHSSSTGEYFTATSSENGEVLIYDLSSQYKAFDSPGHTIPKNAKKPIHTIKNHGNVEGYGLDWSPLINTGSLLSGDCSGRVYLTNRTSSNWVTDKTPFTVDNNESIEDIQWSKAEQTVFATAGTDGYIRIWDTRSKKHKPAISVVGSKTDINVISWCDKINYLLASGDDDGKWGIWDLRNFKPGNQPSPVAQYDFHKSAITSISFNPLDESIIAVSSEDNTVTLWDLSVEADDEEIKQQKSDAKDLEDIPPQLLFVHWQKDVKDVKWHKQIPGALVSTGTDGLNVWKTISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4B0</accession>
    <name>A0A1E3P4B0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Outer spore wall protein RRT8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_14098</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038914.1">
        <property type="nucleotide sequence ID" value="XM_019180595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4B0"/>
    <dbReference type="STRING" id="683960.A0A1E3P4B0"/>
    <dbReference type="GeneID" id="30197841"/>
    <dbReference type="OrthoDB" id="10012223at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005619">
        <property type="term" value="C:ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059112">
        <property type="entry name" value="CysZ/EI24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052786">
        <property type="entry name" value="Spore_wall_assembly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292:SF2">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07264">
        <property type="entry name" value="EI24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="14"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="128"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="26106" checksum="CF252B03DC8BED05" modified="2017-01-18" version="1" fragment="single">GIHYFITHPSLWCYFLAIVIPQMILTATVFVLLFATLNVIQSLLGFAMNGPVGVFIAWVNFIHEVDLVAQVVSTFLLYPKPLQLLFETVLSREGYDDVVLRGKLFRVVDVSIMKRFKRWLLDLPNNLIIYPMTSINFAITVILTFVPIVGPILLVFINAPQRGFGFHSRYYELKGIDTRQKNAFYASRRFHYIGFGIVTGILQNIPFVSTLFQFTSVTGAALLAAEFESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4C2</accession>
    <name>A0A1E3P4C2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Heat shock transcription factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Heat shock factor protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_23713</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-binding transcription factor that specifically binds heat shock promoter elements (HSE) and activates transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotrimer. Homotrimerization increases the affinity of HSF1 to DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039482.1">
        <property type="nucleotide sequence ID" value="XM_019181001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4C2"/>
    <dbReference type="STRING" id="683960.A0A1E3P4C2"/>
    <dbReference type="GeneID" id="30198247"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032993">
        <property type="term" value="C:protein-DNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000027">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF396">
        <property type="entry name" value="FLOCCULATION SUPPRESSION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="11">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00084017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ60275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="93" mass="10819" checksum="F34071D092DDE4F6" modified="2017-01-18" version="1" fragment="single">MLEDDDLKHLIWWSSTNESFVITPGEEFSKALAQYFKHTNVASFVRQLNMYGFHKVSDGNTTENSEVTTWEFRHSSGSFRKGDIDSLKSIKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4C4</accession>
    <name>A0A1E3P4C4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_92366</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039400.1">
        <property type="nucleotide sequence ID" value="XM_019186359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4C4"/>
    <dbReference type="STRING" id="683960.A0A1E3P4C4"/>
    <dbReference type="GeneID" id="30203605"/>
    <dbReference type="OrthoDB" id="4089394at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033481">
        <property type="entry name" value="Dni1/Fig1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28092">
        <property type="entry name" value="FACTOR-INDUCED GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28092:SF1">
        <property type="entry name" value="FACTOR-INDUCED GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12351">
        <property type="entry name" value="Fig1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="88" mass="9845" checksum="BCB8A1A86E15D5FF" modified="2017-01-18" version="1">MVNKFNLALAPITTLIWGLGSMWAHVAQRAGKQLVEEASMNIVMAHIGRKAGAMTWTPFTFLCIVAIGVMMLHLRDLRRQIQEVDPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4H5</accession>
    <name>A0A1E3P4H5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_31155</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Adaptor protein complex 3 (AP-3) is a heterotetramer composed of 2 large adaptins (APL5 and APL6), a medium adaptin (APM3) and a small adaptin (APS3).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038989.1">
        <property type="nucleotide sequence ID" value="XM_019181802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4H5"/>
    <dbReference type="STRING" id="683960.A0A1E3P4H5"/>
    <dbReference type="GeneID" id="30199048"/>
    <dbReference type="OrthoDB" id="10261046at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030123">
        <property type="term" value="C:AP-3 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000001">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="8">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="142" mass="16403" checksum="97AA3A8B07B40877" modified="2017-01-18" version="1">MIHSVLIFNNDCLPRLMKFYTPVDIETQVLLLRQVYQLVNQRQPNQSSFLTPPPLLEGIEDIKVIYRHYATLYFVFIVDDQESELGILDLIQVFVQSLDKCFENVCELDLVFGWQVLQTVLEEIVQGGLVIETNISNIVKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P4N4</accession>
    <name>A0A1E3P4N4_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_62993</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039643.1">
        <property type="nucleotide sequence ID" value="XM_019183473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P4N4"/>
    <dbReference type="GeneID" id="30200719"/>
    <dbReference type="OrthoDB" id="2150604at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052058">
        <property type="entry name" value="Alcohol_O-acetyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037:SF1">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="398" mass="45852" checksum="34F86A55CC0C5922" modified="2017-01-18" version="1">MTISKLNFIRKLGEVEKFFTSITRIGRYNNFSYIVAKQQTYSPEQIHYSLSQTIKSNPILASVVLNDSIYLPDSLKLNQVYSKWSNHIKLNELFSKINGIRFKYSEDSNLFKIVETDSHFAFTMDHSIFDGKSVMSLSKDFLSNLNNTPASQNVNEPIYKFNPKDTISPPLETILHPNSTIPKRLTSDLPRFESGVHSYGLGKHHFQTKVIKKPSNLNMSLTTKIQTSWVKALIEMGFPKSVQYDTCFGVDSRRWIGKLQDSSIRYGCYSTGYFQSIDPLNVDPQLYSDELRNDALTGDILQPFMMYATSGLNWDEEFSKNYKEMHRGCLEITNIGKVEELDSWFVQSTFDLAFVFGINLVVSGDDLRFVFTMVDNEKIDLQKWSGIIDKFEFFMINH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P551</accession>
    <name>A0A1E3P551_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable quinone oxidoreductase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">NADPH:quinone reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_92824</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039617.1">
        <property type="nucleotide sequence ID" value="XM_019186382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P551"/>
    <dbReference type="STRING" id="683960.A0A1E3P551"/>
    <dbReference type="GeneID" id="30203628"/>
    <dbReference type="OrthoDB" id="48317at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003960">
        <property type="term" value="F:quinone reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05286">
        <property type="entry name" value="QOR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000053">
        <property type="entry name" value="Quinone oxidoreductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047618">
        <property type="entry name" value="QOR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106:SF13">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="5">
        <location>
            <begin position="18"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00070796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="334" mass="36594" checksum="0BC52A676BEF2A3E" modified="2017-01-18" version="1">MSTQIPKIQKAIRIHEAGGLEVLHYEDVVVPSINEDEVLIKNKYAGVNFLDTYIRTGLYDAPKPCILGREASGVIVAKGKHVQGFEIGDNVAYASEGTFAQYTKFNPSIRIFKLPKDASEEKLQLYGGLFIQGFTGLTFTTEAYNVQKGDYILVHAAAGGTGSILTQVINARGAHVIATASTQEKLQIAKEKGAEFLINSKTENISKRVMEITKGKGVQASFDGVGKATFQASIDSLARKGTLVTFGNASGPVPPVHLGIMLPKNLKLSRPACFNYLVEKEEWEYYSEKLFKLIEAGDLKLDISKVYPLSEYKQATEDLEGRKTTGKLILKIPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5C0</accession>
    <name>A0A1E3P5C0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RWD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_27657</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039842.1">
        <property type="nucleotide sequence ID" value="XM_019181396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5C0"/>
    <dbReference type="STRING" id="683960.A0A1E3P5C0"/>
    <dbReference type="GeneID" id="30198642"/>
    <dbReference type="OrthoDB" id="277175at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031333">
        <property type="term" value="P:negative regulation of protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040213">
        <property type="entry name" value="GIR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12292">
        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="2">
        <location>
            <begin position="9"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ60635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="211" mass="24352" checksum="22246D7E67D53D81" modified="2017-01-18" version="1">MDYKEEQQQEIEILQSIYPDELEVVSENEYTITLLLDTVSERKHRVILHVKYPETYPEDEDNDEPKLVLISEQVELDQDDLDEIKSNLMQEAEDQIGIPSVFALASSLKDQAEQVFENKVNAKQKQYDDALLAKEREEQKKFSGTKVTVESFNSWRLKFRAEMGINDRIEKRKKMLHRGKLSGKEIFEKGLAGDDETLEELGGDVATKLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5H4</accession>
    <name>A0A1E3P5H4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">NADH-cytochrome b5 reductase</fullName>
            <ecNumber evidence="19">1.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">WICANDRAFT_28537</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">NADH-dependent reductase for DPH3 and cytochrome b5. Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase, predominantly CBR1. By reducing DPH3, also involved in the formation of the tRNA wobble base modification mcm5s 2U (5-methoxycarbonylmethyl-2-thiouridine), mediated by the elongator complex. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>2 Fe(3+)-[Dph3] + NADH = 2 Fe(2+)-[Dph3] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:71231"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="Rhea" id="RHEA-COMP:18003"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:71232"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 19">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46680"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18 19">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer. Component of the 2-(3-amino-3-carboxypropyl)histidine synthase complex composed of DPH1, DPH2, DPH3 and a NADH-dependent reductase, predominantly CBR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="19"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039913.1">
        <property type="nucleotide sequence ID" value="XM_019181501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5H4"/>
    <dbReference type="STRING" id="683960.A0A1E3P5H4"/>
    <dbReference type="GeneID" id="30198747"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090524">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NADH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000032">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000019">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF184">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18 19" id="KW-0274">FAD</keyword>
    <keyword evidence="5 18 19" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="11 19" id="KW-0520">NAD</keyword>
    <keyword evidence="10 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="21">
        <location>
            <begin position="34"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="ODQ60706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="276" mass="30305" checksum="88EA7112E97F3D94" modified="2017-01-18" version="1">MSLPIIAIAIALLAIIGSTVLFLSSKERKVLNKEKFQKFPLIQKTILSHNSGIYRFGLPKPSDILGLPIGQHISISANINGKDIVRSYTPTSNDAERGYFDLLIKSYPTGNISKYIAELEIGDSIDVRGPKGFFEYTPNMCTHFGMVAGGTGITPMYQIINAIISNPEDKTQVQLIYGNQTEADILLKKELDHIVQNHPQIKVHYLLDRPGPNWDGGVGYVTPELLNQFIPAATDKTQLLLCGPPPMVNSVKKAAVNLGFQKAKPVSKAGDQIFVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5P7</accession>
    <name>A0A1E3P5P7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein eL24-related N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_89969</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
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        <property type="protein sequence ID" value="ODQ60769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019186202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5P7"/>
    <dbReference type="STRING" id="683960.A0A1E3P5P7"/>
    <dbReference type="GeneID" id="30203448"/>
    <dbReference type="OrthoDB" id="1727108at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.20:FF:000002">
        <property type="entry name" value="60S ribosomal protein L24"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023442">
        <property type="entry name" value="Ribosomal_eL24_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01073">
        <property type="entry name" value="RIBOSOMAL_L24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL24-related N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="96"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ60769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="128" mass="14850" checksum="F3A696E0473ADB0F" modified="2017-01-18" version="1" fragment="single">MKVEVDSFSGSKIYPGRGTLFIRGDSKIFRFQNSKSASLFHQRKNPRRISWTVLYRRQHKKGITEEVAKKRSRKSVKNQRAIVGASLELIKERRAHKPEFRKAQRDAALKAEKEKRKADKAARKADKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P5W3</accession>
    <name>A0A1E3P5W3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_28550</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040043.1">
        <property type="nucleotide sequence ID" value="XM_019181503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P5W3"/>
    <dbReference type="STRING" id="683960.A0A1E3P5W3"/>
    <dbReference type="GeneID" id="30198749"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF46">
        <property type="entry name" value="SPS-SENSOR COMPONENT SSY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="417"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="502"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="533"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="45"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ60836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="599" mass="66347" checksum="08AC4DFA20C73110" modified="2017-01-18" version="1">MFKTDNSQSFFSTGDFNPHEHKGNYGGGNYNGKYHLQRKLKKQQIQMIALGGTLGVGLFLGSGKAFSIGGPLGCFLGFLISGSIVLATMLSFAEMSTLIPTTSSISGLASRFVEDAFGFALGWCYWLSFAIAMPGEVCAATIMLSYYQDLNIPSASTAGFVSLFLFAILFVNLFDVRIYGNIEYCVSLFKVLITIALIISMIIINVKENYGFRYWNKKDSTDVATYGPFRPTFDLNDIGTGALNGIGGGLGNLLGVVSSILISSYAYTGSEIGFVASMECRNPRKALPSVTKRVFGRVVFFYLLSIFLVGLNIYAGDPRLLRYYSPDAKRGPISIKFLEHLNTNCTPFSQIGSYTNGNQSPWIIALQSSGLCTFSSVLNAVFVVFGVSAGSSHLYVSSRTLYSMATQRKAFAVFTRCTRAGVPYVSVLFSGAFGLLSYLSVNNTSLDVFQDFANISSATAMLMWAGMCLSFLRFYYGLKLRPDIIARDDPSYPYRSPFQPFLAIYGLVGSLLIVILMGFVVFLKGFWDTKTFFTSYGALMLFAICYVGYKFFRSSRIHRLDQLDLDSGRREMDRIIWDEDKYYISSFKEIIKRALGWLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P601</accession>
    <name>A0A1E3P601_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peroxidase</fullName>
            <ecNumber evidence="14">1.11.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_90123</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 Fe(II)-[cytochrome c] + H2O2 + 2 H(+) = 2 Fe(III)-[cytochrome c] + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:16581"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.11.1.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Forms a one-to-one complex with cytochrome c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion intermembrane space</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peroxidase family. Cytochrome c peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.-" evidence="14"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040072.1">
        <property type="nucleotide sequence ID" value="XM_019186212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P601"/>
    <dbReference type="STRING" id="683960.A0A1E3P601"/>
    <dbReference type="GeneID" id="30203458"/>
    <dbReference type="OrthoDB" id="2859658at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004130">
        <property type="term" value="F:cytochrome-c peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000302">
        <property type="term" value="P:response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000005">
        <property type="entry name" value="Cytochrome c peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044831">
        <property type="entry name" value="Ccp1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002207">
        <property type="entry name" value="Peroxidase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356:SF58">
        <property type="entry name" value="CYTOCHROME C PEROXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356">
        <property type="entry name" value="THYLAKOID LUMENAL 29 KDA PROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00459">
        <property type="entry name" value="ASPEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 14" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="15">
        <location>
            <begin position="166"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ60865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="367" mass="41636" checksum="36F57DC08F7BBCBD" modified="2017-01-18" version="1">MSTIQLAKTTFNKKLIYGLLGAGSITIIGSQLYQDQQSKKYYKFPNNNQNHKNYGKALIAGSGLNVALASKDHEGDYQKIYNDIATKIRDNDEYDEYIGFGPVLVRLAWHLSGTFAKKGYQGCPHQTGGSYGGSIRIDKEANDPANNGLQNGRWFLEEFKTKYPWISYGDLYTLGGVVAIQEMGGPKIGWRHGRKDQGEDFAQTSRLPDASQDADYVRGLFGRMGFNDREVVSLIGAHALGSCHVLAPVLPGQKKSSGPGSGYTGRWTASPNFFTNEFYRLLLEDRWEFKDWSGPRQYEDKDGLMMLPTDYALIQDPKYKEWVVKYAKDQDLFFKDFAKDFQKLLELGIEFPRESKVFYFQTLDEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P631</accession>
    <name>A0A1E3P631_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_27689</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040103.1">
        <property type="nucleotide sequence ID" value="XM_019181399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P631"/>
    <dbReference type="GeneID" id="30198645"/>
    <dbReference type="OrthoDB" id="75720at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="199" mass="22859" checksum="AB81BF554DEBB76C" modified="2017-01-18" version="1" fragment="single">FIEKLLFVASALSYLTQRLTRDQYSKRFPVYPSVPFSNIIWYTDCCDLGLMMVLCCQCLKYRRSRHLFQTVSISLAWIVILFLGVSFLILYNATINFPEDIGRYGLLYIDFVDWIWISGAIFGSLKYTSQVSLNWIGMCTKGLSLVSLGTDLLAVSIQLLVRVMKHDHRDETFHFDPVVSKTKMSIAQHPVKQELTGHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P696</accession>
    <name>A0A1E3P696_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_22523</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the epsin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040100.1">
        <property type="nucleotide sequence ID" value="XM_019180950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P696"/>
    <dbReference type="STRING" id="683960.A0A1E3P696"/>
    <dbReference type="GeneID" id="30198196"/>
    <dbReference type="OrthoDB" id="4033880at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16991">
        <property type="entry name" value="ENTH_Ent1_Ent2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000006">
        <property type="entry name" value="Clathrin interactor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF110">
        <property type="entry name" value="EPSIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
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    </dbReference>
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    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
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            <begin position="11"/>
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        </location>
    </feature>
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            <begin position="140"/>
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        </location>
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            <begin position="345"/>
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        </location>
    </feature>
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            <begin position="276"/>
            <end position="343"/>
        </location>
    </feature>
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            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
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            <begin position="213"/>
            <end position="225"/>
        </location>
    </feature>
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        <location>
            <begin position="349"/>
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        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ60893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="457" mass="53011" checksum="21A9135E87FC48BE" modified="2017-01-18" version="1" fragment="single">MSKAFVRSIKNVANGYSSAQVKVRNATSNEPYGPSTSEMEDIADMTYEHVEFLEIMDMLDRRLNDKGKNWRHVAKALTVLDFIVRAGSENVVLWSKDNLYIIKTLREFQYVDEDGHDQGTIIRVKAKELTSLLRDDERLRTERSMRNDRRRRRRPQRSSSFDSNGDSRSNEELDEDDELQKALNESRLTAEEEERRRKANASDDDGLSTALRLSREEEEARRARESQQQNLLDLSDTQAGQQYDVFGNPINAMNTGYLQNAYGNDYMQQLQQQQYLQQQQQQQQQEQEYLQQLQLQQQQQQQQQYLQQQQQQQQQDYLQQQYLQQQQQQQQQYLQYLQQQQQQQQQQQPLKTGSNNPFSINNDSSASTEYSELNTLLATGTGIDTFGNEGNLRIPAQFTKSTNFINSSGTGIRTDTTSANPFMQKQYTGVASTGIIPSYTGYGFGNQSQQPQQRQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P698</accession>
    <name>A0A1E3P698_WICAA</name>
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        <recommendedName>
            <fullName evidence="4">Protein FYV8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_77549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="30201818"/>
    <dbReference type="OrthoDB" id="4081733at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026248">
        <property type="entry name" value="Fyv8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02076">
        <property type="entry name" value="PROTEINFYV8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="458"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="511"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="638"/>
            <end position="691"/>
        </location>
    </feature>
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        <location>
            <begin position="24"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
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        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
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        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
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        </location>
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        </location>
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            <begin position="380"/>
            <end position="401"/>
        </location>
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        </location>
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            <begin position="522"/>
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        </location>
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            <begin position="638"/>
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    </feature>
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    </feature>
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        <location>
            <begin position="673"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="870" mass="98452" checksum="6DBB458453CE1376" modified="2017-01-18" version="1">MSFEENVQRKKSTRWVRASTANYDGDDWVEEEYYEDEDEPVPDLPPIPAEHLQHYQQTTKISPEKTDNDHDDNKNNNNDNNTTTTTRNDYNDDDDANSFDDTINSPQLNLQKFQNIDLDEELPEKEQEHDPVSATVDEYANIIRDDDSEEEEDYKPTTSRFQPLPRSPEVERFETLKEQKTTPVEEENNEIEAQSSSFQPFPEEHHHISKVDHKEGSSTPQELPKHEEPHPVSTPVVIDDAKSVSKESIVEEKTQRDHVHSDFQPFPEDQSNHGAAESTVQDNDVEYEQPPVEEAIEQENTHNDIIPQVEDSEDEEYIPSSNRFKSSILEDETQQKEPVSEDSSIEEIGSTAIPPQIESSEILDEEPKRVDIPPIPSTAPIETQNHIDPVTDSLQLPTSNRQDFDDVSDTDTLHIHDPEEQSSPSIEEIRRELSNTSLHDLTHDNTYFHEHAYLGSLINTPEPEDENEVQFQPQDEKSIDETNDAAPRTLSQYYSSVNDYFDDYADTSDNENLKVNRDSRASVQSSGSLSTGSFSIKSESRYRYSKASDRDSSFNHSNSDVHSLNPAQQLLADNASTNETDQTPKDTNLNPALSINFGHWRPDTDSFRDQFISGTAPPLPQIDNYTRNSMGEIIEDRGSIIEKDKELDSSNDKGDDHEKSLDSLGNTMSTSETDNHSTLSEHKPVGESYKTESNSVLPLHIPLGSNESTELNPGQYFHEKLAATETNLPKEDNEVTKDVPPTTKLNITKISGNKRTNYSIKQISSISDPAKRIESLRNAREEQANLNLGLNEWLVFAKSSAGVETYKSNVHNSHVAQAYADASTMSRKHTLNNVGSFLHKRRVFQDTSSHAQSLAKGIFTRGKKMLKNDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P699</accession>
    <name>A0A1E3P699_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_51760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60941.1"/>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019182677.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="30199923"/>
    <dbReference type="OrthoDB" id="3980107at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="194" mass="22580" checksum="B7200159E6D8EE1D" modified="2017-01-18" version="1">MSTIIALYNRLESIILKQLSKSEFFKHHLDDPISIKITSIGIIILVLIIIYKLIFNIGLHLQVWELPGKEYFIDTPVHCAHVYINGHVIRNFNLNDQIILSTPLKYHIEFAPEDFEDNENPELGSTLGFTRKKLYFLFKDSSFFESLTSNEQKNYKISDVLIYHKKAELKDDSKPLCLHGVETGFKLDVYYNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6B1</accession>
    <name>A0A1E3P6B1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glucosidase II subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_29316</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism; N-glycan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040182.1">
        <property type="nucleotide sequence ID" value="XM_019181594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6B1"/>
    <dbReference type="STRING" id="683960.A0A1E3P6B1"/>
    <dbReference type="GeneID" id="30198840"/>
    <dbReference type="OrthoDB" id="1334205at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017177">
        <property type="term" value="C:glucosidase II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090599">
        <property type="term" value="F:alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06603">
        <property type="entry name" value="GH31_GANC_GANAB_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033403">
        <property type="entry name" value="DUF5110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF54">
        <property type="entry name" value="BCDNA.GH04962"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17137">
        <property type="entry name" value="DUF5110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133724" description="Glucosidase II subunit alpha" evidence="11">
        <location>
            <begin position="20"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 N-terminal" evidence="13">
        <location>
            <begin position="87"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="12">
        <location>
            <begin position="367"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="15">
        <location>
            <begin position="700"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5110" evidence="14">
        <location>
            <begin position="805"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ60975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="913" mass="104703" checksum="0F9F64143A143957" modified="2017-01-18" version="1">MKLFQFFILLTIYLQGAFAVKDYLFKKCAQSGFCNRNRHFAGEVSKNPDYVSKYSIDESTLNYIESNSTILGDIYKKVNDAKSVRLPFQINILENNNIRLRVDEDRSQIEWNNKFLNKERFADAWKYAFKGEPVVSQNYKFNDLQNGKISVIYGDKDQYELEIFTQDFKVVVKYNGETQLVLNDRSFLNIEHFRKEDENEANLTPEESSFNGFHDDFQDSKDDSVPFGPESVAVDVTFKGFKHVYGIPEHADSLSLKDTSESEPYRLYNVDIFEYETDSRLPMYGVIPFMVAHKVGVSAGIFWVNGADTYVDVKKDQESQDEQVLSSKIDSKTHWISENGILDVVIIIGETPTEVTQSYASLSGNVQLPQLFSLGYHQCRWNYNDEEDVLTITENMDKFEIPYDVIWLDVEYTDAKKYFTWKKELFPDPERMLNKLDETGRTLVAIIDPHLKTGYEVSKDVESKDLALLNKDGDIFKGHCWPGESVWIDTFNPKSYDYWTYLFRNSTGLAGDATNFHIWNDMNEPSIFSGPETSSPKDLVNYGGLEIRSDHNIYGLTFHEATYQALIDRYVNKRPFILTRAYYSGSQRSAAMWTGDNMSKWEYLKISLPMILTSNIAGMPFAGADVGGFFGNPSKELLTRWYQAAVFYPFFRAHAHIDSRRREPWIAGEPYTSYMRDAVKIRYSILPLIYTKFYESSKTGVPVINPLFYETPSNPKTFEIEDEFFLGGLLVKPVTDEGAEAVEIYLPDDEIYYDYETFESLQGEGDHKVDAPLEKIPILIKGGSITPRKDRYRRSSKLMKYDPYTLVIALDSNKSAKGSLYIDDGETFNYENGEFLYIDFSLQDGELQAVVKDGSLSVPNSIEKILIVGLENAPASVQVSQGSEWNADVIQETNYAIIKTPGVDLSKDWSIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6U1</accession>
    <name>A0A1E3P6U1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_28231</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040348.1">
        <property type="nucleotide sequence ID" value="XM_019181463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6U1"/>
    <dbReference type="STRING" id="683960.A0A1E3P6U1"/>
    <dbReference type="GeneID" id="30198709"/>
    <dbReference type="OrthoDB" id="5298304at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015225">
        <property type="term" value="F:biotin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901604">
        <property type="term" value="F:dethiobiotin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015295">
        <property type="term" value="F:solute:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905135">
        <property type="term" value="P:biotin import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000399">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000278">
        <property type="entry name" value="Putative MFS transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF33">
        <property type="entry name" value="VITAMIN H TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="301"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="338"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="426"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="467"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="53"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="491" mass="54458" checksum="B334AF5280CF50EF" modified="2017-01-18" version="1">MSEIDHSSLGTTGKNDVQTSTNSKEEEQYITPEFTPEELSKLNKIYLKLDLRIIPALWCLYFLTSFGSSAYGNTLTMNSEKKHSLIQTLKLTTHDTSTASALYYVGYIIFDVPMNIAMTKLAPQAWLSRIVFTVGLVYACYAALDNAGGIIAVRFISGVAGAGTWPGLSYYVSLWYPPHRMTRRIGYYFTAAQLSASAAGLVAAGFQKMDGARGLEGWRWLFLVYGVVTIFVSLLLLVWLPDNDVDRPESRFEIINKFRKLNVYPLTEDERILHEREMSMYNTKVHWGWKKLVEIFLDIKLWPLIMMYFGVVGTGFGLAVFGTTLIKAVLGVSSINVSLLYAPIWLFDLGAILLITPFSDIFKKFRPLIFSVSTVIIIIGLVVCTYAKHKWSRYGGLLISGFGLGPTVPTCMSMTAEIFGAKHGDIGVAVSSALVSGLGNLGSVMATYALYSGWPADKKRTYEYSNMMLVVILGVSIISAGVSTLMSRKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P6Z4</accession>
    <name>A0A1E3P6Z4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GB1/RHD3-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SEY1</name>
        <name evidence="12" type="ORF">WICANDRAFT_83401</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="8">Enriched in the cortical ER. Concentrated in punctae along the ER tubules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family. RHD3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040406.1">
        <property type="nucleotide sequence ID" value="XM_019185536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P6Z4"/>
    <dbReference type="STRING" id="683960.A0A1E3P6Z4"/>
    <dbReference type="GeneID" id="30202782"/>
    <dbReference type="OrthoDB" id="1597724at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048309">
        <property type="term" value="P:endoplasmic reticulum inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016320">
        <property type="term" value="P:endoplasmic reticulum membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000727">
        <property type="entry name" value="Protein SEY1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03109">
        <property type="entry name" value="Sey1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008803">
        <property type="entry name" value="RHD3/Sey1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046758">
        <property type="entry name" value="Sey1/RHD3-like_3HB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923:SF2">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05879">
        <property type="entry name" value="RHD3_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20428">
        <property type="entry name" value="Sey1_3HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="1"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="8">
        <location>
            <begin position="702"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="706"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="726"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="domain" description="GB1/RHD3-type G" evidence="11">
        <location>
            <begin position="39"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="755"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="755"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="767"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="49"/>
            <end position="56"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ61199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="783" mass="89930" checksum="55AB861747032CC1" modified="2017-01-18" version="1">MSESTIVSNVSVQVIDEDKVFQNDQIKKYLKSVIKQDLGLDYHVISVFGSQSTGKSTLLNKLFHTKFDVMNEAQRQQTTKGIWLGYSPEIETNKTESVEKLHQEHVFVMDVEGSDGRERGEDQDFERKAALFALSTSEVLIVNIWEHQVGLYQGANMGLLKTVFEVNLSLFGNKSHKVLLLFVIRDHVGATPLSNLSNTLTADLEKMWDSINKPKNVSPDVKLSDYFDLEFTALAHKLLQPEKFESDVRDLGDRFSDKQRKDYLFKNDYRQNFPIDGWTVYAERCWEQIQSNKDLDLPTQQILVARFRCDEILKEALEAFDAEFSEISKHFDFSKLEGSQLANHFSSLLTLALTAYDTQASHYNKAVYESKRKGLTSDVNLRLQNVFREYIKQLKKSQIETFTKTFTDKNNKAPFLEKSIQARQAALTNFTTQMEPVKKVDPEFFDYQADLEEFSGELDEQLVKVRQTEVDNLVNRVNKKINPIIKNKSLEIFGTPDEQLWDKVLDNFKSTLNNALARYKKDDDTYDFKIGSDPELNDEIAFRIKKSAWVSFDQFIHEYLREENVVAVLKRQFDDAFAYDKDGVPRTWKNETEISLAYREATEFSLGTLPLFSIAKLSNGTEIIPDIPVTDEDDEDEEEPHTFAHLLNSTDQLKIKKSFLKQAEISYRDANRSIVSNIARIPPFMYVLLIVLGWNEFMAVVRNPLLLMLVLLIGVAVIFIHSMNLWGPVNTATNIFIGQAKSSLRNILLDEPGPARVAAQRQQQASETEESYELDDLSNKKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P727</accession>
    <name>A0A1E3P727_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_77869</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040433.1">
        <property type="nucleotide sequence ID" value="XM_019184625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P727"/>
    <dbReference type="STRING" id="683960.A0A1E3P727"/>
    <dbReference type="GeneID" id="30201871"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="230" mass="21591" checksum="DAB967A582DD02DD" modified="2017-01-18" version="1">MYFSAGPASTNGSGVTTSGGASVYPSTDGHGSGATGSPVTFTNSAGEALTGHTSVQSVTGNDGSMSSHTVVVVPGSSSSGIFTTTGSNGDIVTTSGAYSVGGTTVSYSKGAVQGSTSPEGSLPTGAGGNQGSAAASYSKAAITGTASVTGGVDATGAAVGTNSLRKSQISGGVTATSGFAGGNTLSTSSISAGATSAVAGVSTYEGGASSNLQSKGLFLSLLSLVLFFAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7B9</accession>
    <name>A0A1E3P7B9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_90671</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040490.1">
        <property type="nucleotide sequence ID" value="XM_019186253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7B9"/>
    <dbReference type="STRING" id="683960.A0A1E3P7B9"/>
    <dbReference type="GeneID" id="30203499"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ61283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="468" mass="51369" checksum="5F5F3F00F16A5C4C" modified="2017-01-18" version="1">MGTPPQELSLRIDIAQPYVWTRSRQSLPVCANGQTPNNTFECDSGIGAFNQSASSSYYDENNWQRLIFLDYVWVNGTMSQDTLAFDQAFNVDINGSETRNLTVNEINFINANDSRVPMGGLGLSSKFDDSNNDYNTNFLDILKDQHVINSSSFSLYHKDDSSADLMLGGVNQDYYVGDFVVFDKQPYWDNRENKLVNDYPILPLSSLMVSNARGQTAYISTSNNTEPVLIDTRSSYNYLPYSMVVALAVQLNAFYSNDIDVWLLKCSITNLNATIGFQFGNVTVNVPIDEMINPAYTSSGNETLVFDDGEEACLLLVTPNYYFGYSVLGSPFFKSAYIAVDNEANKIAIAQAAKGNHTREQSSTTTDFAASAISSGHIPFASPNNITQLYTMRFDQSSIKSSAQPDMVTVSITNGEVFTGRVQPSNSSSMSSSASGGGVQLNNHYNNGNSVLGYVFSFITVVGLGILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7I1</accession>
    <name>A0A1E3P7I1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_90071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040044.1">
        <property type="nucleotide sequence ID" value="XM_019186209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7I1"/>
    <dbReference type="GeneID" id="30203455"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ60837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="141" mass="15993" checksum="C425B8260CBA88EB" modified="2017-01-18" version="1">MAIYVSCFPQKQFCIYCKCIKAYHQKLIYEKNSQGYEVNDNDIKLAKSYRDISQLEKGQLNESPEPQDGAAIESAFNFAVDELKAGVNDDYVRLYPTLLFCVNGKIMARYVKGATIFKWQSFIGSVVCGVAVVGFLTLVYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7J0</accession>
    <name>A0A1E3P7J0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_61461</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0357 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ60902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040109.1">
        <property type="nucleotide sequence ID" value="XM_019183181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7J0"/>
    <dbReference type="GeneID" id="30200427"/>
    <dbReference type="OrthoDB" id="447314at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018559">
        <property type="entry name" value="DUF2015"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28023">
        <property type="entry name" value="UPF0357 PROTEIN YCL012C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28023:SF1">
        <property type="entry name" value="UPF0357 PROTEIN YCL012C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09435">
        <property type="entry name" value="DUF2015"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ60902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="132" mass="15045" checksum="4D846AD133669CB1" modified="2017-01-18" version="1">MLVGKFHIGGGILLIIVILILFHLRERWLPKLRVWLGKRRIRLDGYSHLPSFQRDIESGLTSSNFDLVMNNSGDTRKGLDENAKSKILKIMEDNPGLSFDEARLKYTREELSKNQIGPDGIPLDPRTVTFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P7V7</accession>
    <name>A0A1E3P7V7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">Postreplication repair E3 ubiquitin-protein ligase RAD18</fullName>
            <ecNumber evidence="5 17">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">RING-type E3 ubiquitin transferase RAD18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">WICANDRAFT_104146</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">E3 RING-finger protein, member of the UBC2/RAD6 epistasis group. Associates to the E2 ubiquitin conjugating enzyme UBC2/RAD6 to form the UBC2-RAD18 ubiquitin ligase complex involved in postreplicative repair (PRR) of damaged DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with E2 UBC2, forming a complex with ubiquitin ligase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the RAD18 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5 17"/>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040209.1">
        <property type="nucleotide sequence ID" value="XM_019180399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P7V7"/>
    <dbReference type="STRING" id="683960.A0A1E3P7V7"/>
    <dbReference type="GeneID" id="30197645"/>
    <dbReference type="OrthoDB" id="9049620at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097505">
        <property type="term" value="C:Rad6-Rad18 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000172">
        <property type="entry name" value="E3 ubiquitin-protein ligase RAD18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="33"/>
            <end position="70"/>
        </location>
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        <location>
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            <end position="289"/>
        </location>
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    </evidence>
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    </evidence>
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        </source>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000094112"/>
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    </evidence>
    <sequence length="408" mass="46379" checksum="6B0A36F86C37C535" modified="2017-01-18" version="1">MNLLGDLSNITDPLDFKTTKIPKLFEIDSLLRCHICKEFLSAPMLTSCGHTFCSVCIRKYLIHTPKCPICAKELRESNLSRNVLLEQVVLSYRSVRDDLLENLKIAKEIPKVKNNEPIQHSYDLGSNSNSIEVLDKEDEDYDDDVIIIDEPKKRKSEANGIESLFKKHKSETEKKGTCPICNRLLPLKVLESTHIDACLANPEPEVAIVPQPSTSSPSKRSTSIPTQSSTPASPSPYFQPKENNSNLKKLTKLDYPSLSTSGLKQKLTKLELPVAGTRNQLEQRYNEYLILWNANCDSVVPKNPKILRKQLAQWENSLKFKNGNSEKQLDENGWNQLIAQAKKTALKNKKEEKTEEKTEEKKESEKPKDENLETGKEEEDKKESVEEEKVEAIDDSEADTTNEQFFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P891</accession>
    <name>A0A1E3P891_WICAA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">WICANDRAFT_78063</name>
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        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
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        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
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    </dbReference>
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    <dbReference type="OrthoDB" id="2103031at2759"/>
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    </dbReference>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="168" mass="19770" checksum="9A17162748C44979" modified="2017-01-18" version="1">MVWPFSQNKGSSTLRSEKKAEEELLKELPEDLGKFLKFKNRERFKFEDKADDSKFKLHKQQNPVKAIPLTNCADFQLAMVNCLNNGSTTDKLTMCNKESKRYQDCFKLQRNLLLKLGIEHANSIEKYDKIERAADDIGVQWFEEVKDPEVIPDKVLEHVYDKRDEIWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8B2</accession>
    <name>A0A1E3P8B2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
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    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_61677</name>
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    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
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        <lineage>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
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                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
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                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="ODQ61107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019183229.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3P8B2"/>
    <dbReference type="GeneID" id="30200475"/>
    <dbReference type="OrthoDB" id="433512at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:phosphate transmembrane transporter activity"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="F:selenite:proton symporter activity"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097080">
        <property type="term" value="P:plasma membrane selenite transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006797">
        <property type="term" value="P:polyphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Inorganic phosphate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004738">
        <property type="entry name" value="Phos_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00887">
        <property type="entry name" value="2A0109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="339"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="384"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="513"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="61"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="548"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="548"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ61107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="577" mass="62832" checksum="F9E2433B00B1C96D" modified="2017-01-18" version="1">MASTIDEKKNPAEVGIKTKGGNSAFHNYLNDFSHIDDPLERRRLALEKIDQASFGWYHVRAITVAGVGFLTDAYDIFAINLGLSMIAYVYWGGSIPSSTSTLLKVSTSVGTVIGQLGFGFLADYVGRKKIYGTELMVMIAATVAQCVIGESKAISFPAIMTFFRIIQGIGIGGDYPMSSVISSEFATTKWRGAIMGAVFANQGWGQLAAGLVALICVAGYKDQLIVANKASECTGDCIRACDQMWRILIGFGCVPGCIALYYRLTIPESPRYTLDVEYDIEKAVADSGKFASGAHGNADNEDVQQLRVTERVKAEIIQPPKASFKDFCTHFGQWKHMKILIGTAGSWFMLDVAYYGLGLNTATILQTIGYASSKNVYHSLYNQAAGNLILICAGSIPGYWFSVATIDTIGRKPVQLGGFLLLTIILCIIGFGYHKIGEHGLLGLYVIAQFFQNFGPNTTTFIVPGECYPTRYRSTAHGISAACGKVGAIIAQTALGTLIDHNCARDGKKKNCWLPHVMEIFALFMLVGFFISFLIPETKRKTLEQISEEVHGEVDPSKMARSNAEHYPETSSGDHIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8H7</accession>
    <name>A0A1E3P8H7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">WICANDRAFT_77894</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 14">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454209">
        <property type="protein sequence ID" value="ODQ61252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040459.1">
        <property type="nucleotide sequence ID" value="XM_019184630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8H7"/>
    <dbReference type="STRING" id="683960.A0A1E3P8H7"/>
    <dbReference type="GeneID" id="30201876"/>
    <dbReference type="OrthoDB" id="1662883at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045298">
        <property type="term" value="C:tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045143">
        <property type="term" value="P:homologous chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000008">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 14" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="15">
        <location>
            <begin position="50"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="16">
        <location>
            <begin position="249"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ61252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="449" mass="49871" checksum="18B76761CC1C692E" modified="2017-01-18" version="1">MREVISVNVGQAGCQIGNSCWELYSLEHGIQPDGYLQEGLNRPKGPEEGYSTFFSETGSGKYVPRALYVDLEPNVVDEVRTGAYKDLFHPEQLITGKEDAANNYARGHYTVGRDLLDDVLDRIRRMSDQCDGLQGFLFTHSLGGGTGSGFGSLLLEQLSLEYGKKSKLEFAVYPAAQTSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDICRRNLDISRPSFANLNNMIAQVVSSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVGYAPVVSKSRATHESNSVSEITNACFGPGNQMVKCDPRVGKYMATCLLYRGDVVTRDVQNAVAQVKTKKTVQLVDWIPTGFKIGICYEPPTAPPSANLAAVSRAVCMLSNTTAIAEAWRRIDRKFDLMYSKRAFVHWYVGEGMEEGEFTEAREDLAALERDYEEVGIDSIDQEEGEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8U2</accession>
    <name>A0A1E3P8U2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_25335</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041043.1">
        <property type="nucleotide sequence ID" value="XM_019181132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8U2"/>
    <dbReference type="STRING" id="683960.A0A1E3P8U2"/>
    <dbReference type="GeneID" id="30198378"/>
    <dbReference type="OrthoDB" id="2108at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050596">
        <property type="entry name" value="AspAT/PAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383:SF1">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="79"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ61836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="467" mass="53095" checksum="B5E9FAEAC589BD24" modified="2017-01-18" version="1">MVPDIPLNGHRTPKKTPLNNFKEEVQRNKSRERTQSPIPIPGPLSDPNVHFPALNKQNAATGVIWCTDRASERGFPNPNWVNLGQGMPETGDIEGCFERPKNLNISVESREYSPTSGIKQLRTAIANLYNEHYRQGKQSKYTWENVNVAPGGRAALTRIAAVLGDAYLSFFLPDYTAYAEMLSLFKNFAPIPVPLYETDGYKIDLKIIKGELNRGVSAILTSNPRNPTGQAMTREELHELHNLCRQKCLLIMDEFYSRYYYDGEMGESISSASEVDDVNKDPVLIIDGLTKFYRLPGWRVSWIVGPKEYISALSSAGSYLDGGTNYPFQEAAIPFLEPNLVKQETIALQTHFKFKRDYCLDRLIKMGFKFVVKPNSTFYIWVDLNHLPGKLSNCLGFFHEALHEDVIVVPGIFFDLNPLSFRELSDSAMYSYIRISYGPEFENLVKGMDRIEKILRRFDALPKDYDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8W2</accession>
    <name>A0A1E3P8W2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dienelactone hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_82023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041048.1">
        <property type="nucleotide sequence ID" value="XM_019185319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8W2"/>
    <dbReference type="STRING" id="683960.A0A1E3P8W2"/>
    <dbReference type="GeneID" id="30202565"/>
    <dbReference type="OrthoDB" id="17560at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630">
        <property type="entry name" value="DIENELACTONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630:SF44">
        <property type="entry name" value="PROTEIN AIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dienelactone hydrolase" evidence="1">
        <location>
            <begin position="32"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ61841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="246" mass="27441" checksum="14A6100DBE525022" modified="2017-01-18" version="1">MSVNLTHHCTETNFHSGESVGKIQPEYLTFKDVYVVGDESKNKGVVVILTDIFGNKYNNNQLVADAFAKAGYYALIPDLFDGDAVELENYGKPGYFDTWKKNHGFNVTSPYVKDFLKKIDSELKPTKIFSVGHCYGGKLVIQNLTKDGLLAAGAVAHTSGTELSEVKEIINPIIFSCAENDPAFTDELRTQSQQILKENKIKYQFDLFSGVAHGYAVRGDPNNPDDKYASEKTLKDQIAWFDRFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8W6</accession>
    <name>A0A1E3P8W6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_48765</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019040957.1">
        <property type="nucleotide sequence ID" value="XM_019182615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8W6"/>
    <dbReference type="STRING" id="683960.A0A1E3P8W6"/>
    <dbReference type="GeneID" id="30199861"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000017">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004762">
        <property type="entry name" value="Amino_acid_permease_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00913">
        <property type="entry name" value="2A0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF1">
        <property type="entry name" value="GENERAL AMINO-ACID PERMEASE GAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="394"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="440"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="10">
        <location>
            <begin position="45"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ61750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="548" mass="59838" checksum="31459DD592FF00B6" modified="2017-01-18" version="1">MGRFVDSFRQMEFDESLLKPGMTEAEKTEVRLANTPLQKSLKKRHLQMIAIGGSIGTGLFVGSGSALRTGGAASLPIAWVVVGTFLFAVIHALGELAVAYPVPGAFSAYATKFIDPSWGFAIGWNYVFLWLVVLPLEIIAAALTINFWDDTINSVAWVIIFYVLIMGINLFGVKGYGEAEFLFSLIKVVAICGFLILGIVLVCGGGPSGGFVGGEYYRDPGPFTNGFKGFCTVLVVASYALGGSELIGVTAAESENPRKTLPSAIKQVFWRLMLFYLGGLTLVSLLVQATDPRLLGSSSVDASASPFVIAIKNGGVKGLPSVMNVVILISVLSVGNSAVYGCSRTLTSLAAQGLAPSIFGYIDRMGRPIFGIILNGITGLLCFIVANRKIQSEVFTWLLAISGLCILFTWLTISICHIRFRLAMKAQGRSLKSLAFTSHAGFWGSVYAVVMMSLVLIAQFWTSVWPLHGKPNARVFFENYIGAPIFISFYIARKLWVRKFNEFTPLADIDLDGGRKDLDMDLLQQELAEEKAELQTKPLWFRFYKFWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P8X5</accession>
    <name>A0A1E3P8X5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_48974</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041073.1">
        <property type="nucleotide sequence ID" value="XM_019182622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P8X5"/>
    <dbReference type="STRING" id="683960.A0A1E3P8X5"/>
    <dbReference type="GeneID" id="30199868"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000297">
        <property type="term" value="F:spermine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF132">
        <property type="entry name" value="POLYAMINE TRANSPORTER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="415"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="450"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="492"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="516"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="550"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="585"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="185"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="110"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ61866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="626" mass="69096" checksum="C3F9AABA0B3F7D06" modified="2017-01-18" version="1">MSTHHSHSDIESINSQAQLDQAPQAYVPEDNQDAVSELSSFASRTTTNNNVRLVPTETVKSLQDMGVQSDAPIPDVNAPQSVKNAIFPEEYTLETSTGLVPVATLQSIGRTGTQVSRTRTAHLARRSTHIDDQEDESSSNTNVEKKEGEETPGSDEIDPEIEFVTFVTNDPENPHNWPLWLRWVYTVNLSILVIAVAFGSSCVTGGLGLINDKFHVSLEVSILTCSLMVFGFSLGPLLWSPLSEQIGRQPVYFISLLLYVIFNIPCAVAKNIGTLLVCRFLCGVWASSGLCLVGGSIADMFPTETRGTAIAMFSFAPYCGPVFGPLICGWINVKTGRFDVIIWVNLAFAGFMWITATLVPETYAPVILKRKAKRLRKETGNPKIMTEQEAQGLSFKEIVQSCLVRPLYFVISEPVLDLVCFYVCLIYALLYAFFFAYPVIFSELYGYNDAYIGLMMIPILIGAVLALVVTPIIERQYVKTAHKRKMTPEDRLVGGMIGAPFAAIALWILGATSYKHIIWVGPASSGLAFGFGMVLIYYSLNNYIIDCYAIYASSALATKVFLRSAGGGAFPLFTTQMYHKLGLQWASWLLAFISTAMVLIPYAFYFYGKTLRGKLSKKNYSLDETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9I8</accession>
    <name>A0A1E3P9I8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_82190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041283.1">
        <property type="nucleotide sequence ID" value="XM_019185345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9I8"/>
    <dbReference type="GeneID" id="30202591"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133770" description="Secreted protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="70" mass="7619" checksum="F6CB84ECA9FA5B0E" modified="2017-01-18" version="1">MPLITRLLCCVLILGPGSLSLVVHSHKVVLRVFEQVKNILLINRVAIELTTGSTTKPADKVNVDNGLFIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P9N1</accession>
    <name>A0A1E3P9N1_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Anaphase spindle elongation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_82235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041332.1">
        <property type="nucleotide sequence ID" value="XM_019185357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P9N1"/>
    <dbReference type="STRING" id="683960.A0A1E3P9N1"/>
    <dbReference type="GeneID" id="30202603"/>
    <dbReference type="OrthoDB" id="642895at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051256">
        <property type="term" value="P:mitotic spindle midzone assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007145">
        <property type="entry name" value="MAP65_Ase1_PRC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321:SF41">
        <property type="entry name" value="FASCETTO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321">
        <property type="entry name" value="PROTEIN REGULATOR OF CYTOKINESIS 1 PRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03999">
        <property type="entry name" value="MAP65_ASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="546"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="692"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="641"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="728"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="749"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="801" mass="91666" checksum="A8CA1635FCA435D6" modified="2017-01-18" version="1">MENNCFTNRMNEESISSGRLNLISSPVKISLLGTNTKQDTTPIQMESTISQAEGKENFAMISTQFSSLVQQLSKIYTEIGYSKSDVSKNEKKLFSSVSNTLDQFLNDSINFKDSLIKENGEIIQNLKIILNILDDQTGSRTIPDLYIRNLILKPSQENSTLLSIRKSLEMAITHVSKKYQEIMLKYLAQCLGLNKLLERLENHNFKEIQIPNLDNSISICNVFESATSPSEIYEMISADPKTFFENESLKDLSIRKFESISDQIKIHEQFLKERINFLKEKSNEILGLWETLAIDYTVDYASLSDQVIFHAKSDADVESLTVGASVVNQFDQILGELKELYETRFQQKQKYQVQCEELWVKLNEDETQIAHFKQSNQSLSIQSIDNYSRELQRLLELKKLLLKDLILDARNTIETLWDKLLYSTGERDKFTAFHQETFDDDLLTLHESEIEILNKKYESYKPILESVQQFKDLMSDKKKLEESSKDSSRLLQRNSHKILMEEEKIRKKLARQLPKIVSSLKLKLSEFEKDYGTSFSFLDTELMSELEEQEQSLESKNSRRVVSGGSTAPTRSSSRAPTRPTTRAPSRQPSRQPSRMTSRATSRVTSPTKPVPATTNPRYPPPTQGIRKPAVPTFKRPTLGTKPSIQTSQSCLLPKLSSPPKDTKLNIMPDMIKDHTRAGIRRTPIRAIPSFQRSTSSFQPRLSRLDEQVSGSPKARRTRLSPLKESTRSNLPVLSKGSNNIVTSPLKSLKKDGYSSEEKEHKAMDLISSDDEEDNDTYSRWRREQLQKLNKPSINLSQDTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PA51</accession>
    <name>A0A1E3PA51_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_22010</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041490.1">
        <property type="nucleotide sequence ID" value="XM_019180924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PA51"/>
    <dbReference type="STRING" id="683960.A0A1E3PA51"/>
    <dbReference type="GeneID" id="30198170"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000433">
        <property type="term" value="P:carbon catabolite repression of transcription from RNA polymerase II promoter by glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051007">
        <property type="entry name" value="creA/MIG_C2H2-ZnF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428">
        <property type="entry name" value="REGULATORY PROTEIN MIG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428:SF2">
        <property type="entry name" value="ZINC FINGER PROTEIN RSV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ62283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="73" mass="8446" checksum="F845A9466EE1B228" modified="2017-01-18" version="1" fragment="single">SNSTTNINEAGKKPKKTHSCPLCAAVFQRPEHVKRHMRSHSSEKPYECDEPNCGKRFNRGDNLKAHLRKIHHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAA1</accession>
    <name>A0A1E3PAA1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_98834</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041540.1">
        <property type="nucleotide sequence ID" value="XM_019186671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAA1"/>
    <dbReference type="GeneID" id="30203917"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="89" mass="9400" checksum="1B07CF421CC2F279" modified="2017-01-18" version="1">MIPSGNSSVAAEDQLKMSIKSSSTTSSSSFDSTPLQSLGPNSASHISDPHGGSSIEKHPKYDLANLGLNIVGMDKEGTPRCYKDTQLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAK2</accession>
    <name>A0A1E3PAK2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein transport protein SEC31</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Protein transport protein sec31</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">WICANDRAFT_87581</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPII-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat SEC31 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041461.1">
        <property type="nucleotide sequence ID" value="XM_019186118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAK2"/>
    <dbReference type="STRING" id="683960.A0A1E3PAK2"/>
    <dbReference type="GeneID" id="30203364"/>
    <dbReference type="OrthoDB" id="542917at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902953">
        <property type="term" value="P:positive regulation of ER to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070863">
        <property type="term" value="P:positive regulation of protein exit from endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.940.10:FF:000007">
        <property type="entry name" value="Protein transport protein (SEC31), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.940.10">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040251">
        <property type="entry name" value="SEC31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009917">
        <property type="entry name" value="SRA1/Sec31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923">
        <property type="entry name" value="SEC31-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923:SF11">
        <property type="entry name" value="SECRETORY 31, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07304">
        <property type="entry name" value="SRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="7 15" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="103"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="246"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="SRA1/Sec31" evidence="17">
        <location>
            <begin position="1111"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="781"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="859"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="1046"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="1065"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1079"/>
            <end position="1133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF07304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ62254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1246" mass="133407" checksum="722FA854C51B36D0" modified="2017-01-18" version="1">MVKINDINSTATFAWSNETLPTLATGTAAGVIDDDFSSNSVLSFYDLEDKEPKVTINTDAKFHDLDWSKNNTLLAGALENGTVQFWNTSKLNSEISVESVATGTKHTGAIKSLQFNPLQHNILASGGANSEIFIWDTNKLTNLAPFAPGTAMTPMDTVQSVSWNNSVSHIFASAGSAGYTSIWDLKAKKEVLHLTYTNNTGSRANLSVVAWHPTQSTKLITASESDGLPVLLTWDLRNSNAPESILTGHKKGILSIDWNTKDPEFLLSSGKDNTTILWNPISGQKLAQYPTTANWVFKTRFAPSSPDIFASASFDKKISIQTLQDTSPPAQNRINSTTENDFWNQISDTDTQQPEYFIKQAPKWYGAHSAVGFGFGGKIVTVTKTGDKQSEVKISKVKVPGSKDNENLKTALNTGDFKSLIESHLKDSHDDVSKSDWELLNELTDKSDVFEKFISLKISTDDEKDAEGEESTNGEDFFNNLGDSNQAKEYLPSGPFKLVEQDSKFTDLSNALLAKDLNKAVDISLSNDKLVEALIISQNGSEELKQKVKNAYFAKYASKDPLARLLYSVSSDNVNDLVENGDVADWKQIGTAIKTYSKNEELSKTQFAKLGDRILASDPVKNRNDAIKTYITASALDKVAAIWVSELKGLETKILKSSKKSAYDAHFESLTEFVEKFSAYRSILKLDAPVTDNEALLSTILEFTNIISTSGQFELANKFLGLLSDQIPAVKLEKERIAKASGIRTTNPTTSTTRATGAGRYGSVGGASGAARAAAVPSANPYTAGAQPQIPGFPPAPVAAQSPYAQFSQPPVPGPPAASNPYRPSAAATPSIPQAAPLASSNPYKPAASIPSNPYKPVDVATPVGPPGSVGSVPPPPPKIASKKNETEGWNDLPSTFHRQPRRGTPAAAVNVATPYGAASPSQGAASLAPPISRTSSFVNPPSIPPPPRSISKSRVPSVQENKPSPPPAVNNRYAPTQPSPQPNATPLGTNGSFPPPPPINGSPYGAPPAAAQTPKNPYAPTTAPSISPVSNPYAPPQPANVAPPGAKPTPYAPPPQSTPAINPYAPPPASSIPPPPAQGAYAPPLAAGQASATPLTAAASLQQHAQYQAAQPPQEQQQPPQASSAPPKSKYPAGDRSHIPAATQNIYQSLSKELEQVKPSIPEKFNKQVLDTEKRLNILFDHLNNEELLTQPTIDLLNSLIDSLNSKDYQTALNIHIDLMTNHSHEGGNWLVGIKRLIQFSEAVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAQ8</accession>
    <name>A0A1E3PAQ8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein ORM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_27585</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041655.1">
        <property type="nucleotide sequence ID" value="XM_019181391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAQ8"/>
    <dbReference type="STRING" id="683960.A0A1E3PAQ8"/>
    <dbReference type="GeneID" id="30198637"/>
    <dbReference type="OrthoDB" id="1932233at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007203">
        <property type="entry name" value="ORMDL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12665">
        <property type="entry name" value="ORMDL PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04061">
        <property type="entry name" value="ORMDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018147">
        <property type="entry name" value="ORMDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ62448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="21117" checksum="273E6418F4F28FCE" modified="2017-01-18" version="1">MSEPSAQTLSPNSSNARQHRRRSSSIISHVEPETFEEKIDQESTPNLNANWVHSKGAWIIHIVIILILKLFFDLVPGLSNEISWSFTNATYVIGSYIMFHYVKGTPFDFNSGAYDNLTMWEQLDEGDFYTPSKKFLVGVPIWLFLCSTHYSHYDLKLFIINLLICAVGVVPKLPIFDRLRISLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAR7</accession>
    <name>A0A1E3PAR7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Alpha-1,3-glucosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_82384</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate = an alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31307"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="Rhea" id="RHEA-COMP:19521"/>
            <dbReference type="Rhea" id="RHEA-COMP:19522"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:132521"/>
            <dbReference type="ChEBI" id="CHEBI:132522"/>
            <dbReference type="EC" id="2.4.1.265"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:31308"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ALG6/ALG8 glucosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041531.1">
        <property type="nucleotide sequence ID" value="XM_019185371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAR7"/>
    <dbReference type="STRING" id="683960.A0A1E3PAR7"/>
    <dbReference type="GeneID" id="30202617"/>
    <dbReference type="OrthoDB" id="1689333at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042283">
        <property type="term" value="F:dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004856">
        <property type="entry name" value="Glyco_trans_ALG6/ALG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413">
        <property type="entry name" value="DOLICHYL GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413:SF2">
        <property type="entry name" value="DOLICHYL PYROPHOSPHATE GLC1MAN9GLCNAC2 ALPHA-1,3-GLUCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03155">
        <property type="entry name" value="Alg6_Alg8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="184"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="409"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="433"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="471"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="502"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ62324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="543" mass="62739" checksum="408705F49227D3DD" modified="2017-01-18" version="1">MSTLKNRKEKGKNFSLWNIWVAATILKVLLFDTYHSTDFDVHRNWLAITNKLPLKDWYLEKTSQWTLDYPPFFAYFEWFLSQFVPQIVKDDGCLDIVEKGAYGWPTVVFQRSTVIISEILLFAILQTYINFSKDKILSFIIASSLVLSPGLLIVDHIHFQYNGMMFGLLIGVIVAAAHERYYLLGALFATLLCFKHIFLYLAPAVFVFLLRKVVLDVSQKSIIKFIKWDKLIKLGSIVLFIFGVAFAPFVYYGVIPNLLARLFPFSRGLTHAYWAPNIWAIYSFLDKILVVVLKRPYFNNVLAKAIGTPSIAVIDAQIASTANTGTRGLVQDVFFIILPNITPRVTFLLTLFYDILSLLPLLLQPNFKRLIGALTLNAYASFLFGWHVHEKAIMLIIIPFTFLINERRLLNSFLVLAGAGYVSLFPLLPNQNLIKQLYTFIWFIIYSLSFMEVSKQRTFKKRIFLFDRISLIYLFSLIPMVLMVFIIGLFKKRYALFAKFEFVGLMIYSVYCGIGVIASWCGLSWLYFFEDSIWSDEDEKKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAS2</accession>
    <name>A0A1E3PAS2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_76222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041249.1">
        <property type="nucleotide sequence ID" value="XM_019184344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAS2"/>
    <dbReference type="GeneID" id="30201590"/>
    <dbReference type="OrthoDB" id="3980787at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="399" mass="44952" checksum="9EF637B84497600F" modified="2017-01-18" version="1">MRDNKPSSIHSTSTIRRAHKSNVSPSFQHRSIKSTFSPTSLKPQIYKDTETREPSKYTQIFAHEQQILKNKKSRLDMLDNSQLTSFPLAPPPSSEKVTESLKSPEINAQEIPSSRPDSQLFEIDPIVLVEDYIKAKELGRRKLSVVDLKTSLKTYESMNPADLPEGPNYLTKAKSILSETISSHQSQLGTSQTSSLLLTKCSAEEEGSIATTDFYSAKTSQTLSSACSIHTNISNAPTLKLTDSHDMTLGEFTMPSASSSLKYCVICETPLYELSSLLTQDSQFTEFVCSNCTERYEMLSNLIDNFDHSISELRAIPEDASIDDIEELLNQPANKKSRNNDLHGFSSTLVSKLQNQLEYKGTTQRQQIDQKSAIWLLEAKKKLRWRWRISGLIPQFLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PAT9</accession>
    <name>A0A1E3PAT9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_24775</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ61987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041194.1">
        <property type="nucleotide sequence ID" value="XM_019181056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PAT9"/>
    <dbReference type="STRING" id="683960.A0A1E3PAT9"/>
    <dbReference type="GeneID" id="30198302"/>
    <dbReference type="OrthoDB" id="2155291at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000935">
        <property type="term" value="C:division septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035838">
        <property type="term" value="C:growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030950">
        <property type="term" value="P:establishment or maintenance of actin cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04399">
        <property type="entry name" value="RhoGAP_fRGD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF17">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN RGD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="5">
        <location>
            <begin position="2"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="3">
        <location>
            <begin position="233"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="460"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="698"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="698"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="721"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="741"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ61987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="763" mass="86800" checksum="DED601DF935F6CA0" modified="2017-01-18" version="1">MPSFADSFWTPNYSSGIEVLFTKLNQGCIENDEFINLFTSRMDYEFDYGKKLSALPDIHSPMKGGFDKDDGASLKNAFKGLALEMGNEGDSHLQIALNIKEMVIDPFSKWSQEHKQRIDYSEKILKSSLKVYKNKLKAVEKIQKKYFNKCRTLESIKSGMTEEEITKELNSIDLTDSQEQKGKSNPSDADGEEYEPLVVLGGVEYGKIGLKELIRSMLTEVPQGSFKVAILGVYEHVSTGSSIVSFLQQQLGITNIDKCERFGKDLITNGFIRHVNGVGSNFVNSSTFQYQWKSTAFQIADLPIKKNEADDDDENLNNRKVSNYIDELKNAISYEETSLKKVDNDVKELDAAYKIEVAKLDKIRCDLEELIVDHLSFMEKCELDRLRALKKVLLDFSASISNKLSNIKAIIDKIMIYEETINPTGDLMFLLQNYRTGSFVPNVVLYDNYYDSFKDQIFGVDLETRCKNDNKTVPVIISSILSYMDNIYPELPNDDVRTKIWLIPVRLQSTHALRSEIEKLGSTGLTPEFFKNFEPEVIASVLKLYLLELPDSVVPNGLYDLIRSIYNQYGSESESKERINGITNVLKNLDRSNLSTLNAICTHFTRLIKILLENKNENESNGLASSFQNGISQEFSNCILRPRYQNNLSLSDKHSFRFFHDLLEHKAEIFKNLKPTGSIKKNKSVLAGVEERITRSEQLSRQSSKLQSRLQQAVITGSRRVSNEAKSNESDHVRNLTPQNDNEKENNNHSEEQSNAENPIVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PB24</accession>
    <name>A0A1E3PB24_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">60S ribosomal export protein NMD3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_76319</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as an adapter for the XPO1/CRM1-mediated export of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the NMD3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041349.1">
        <property type="nucleotide sequence ID" value="XM_019184365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PB24"/>
    <dbReference type="STRING" id="683960.A0A1E3PB24"/>
    <dbReference type="GeneID" id="30201611"/>
    <dbReference type="OrthoDB" id="203821at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048899">
        <property type="entry name" value="NMD_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048898">
        <property type="entry name" value="OB_NMD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746:SF2">
        <property type="entry name" value="60S RIBOSOMAL EXPORT PROTEIN NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21193">
        <property type="entry name" value="NMD_SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21192">
        <property type="entry name" value="OB_NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nmd3 N-terminal" evidence="10">
        <location>
            <begin position="20"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 SH3" evidence="12">
        <location>
            <begin position="252"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 OB-fold" evidence="11">
        <location>
            <begin position="316"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="463"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="431"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="478"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ62142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="509" mass="57943" checksum="22F5AA077B489E6A" modified="2017-01-18" version="1">MSYMPLQHDPQQQTAATVLCCNCGVPMDGSTGLVMCYDCIKLTVDITEGIPRESNVSFCRNCERFLQPPGQWIRAELESRELLALCLRRLKGLNKIRLIDASFIWTEPHSRRIKVKLTVQGEAMSNTIVQQSFEVEYVVIAMQCADCAKSYTTHTWRAAVQIRQKVAHKRTFLYLEQLILKHNAHVDTISIQESKDGLDFFYSSRSHALKMIDFLGSVAPIKYKRSEELISQDIHAGTSTYKFSFSVEIVPICRDDLVILPKKLAHQLGGISQIVICSKVSNTIQFLDVNTLQIADVSPTVYYKKPFPSLAEASQLVEFIVLDVEPTGQSKGKHVLADVTCARAADMGVNDREFYVRSHLGGILHPGDSVMGYYTTNSNYNHELWDTLDPSNVPDVILVKKFYQRKTRKNRNWKLKRMAKEHADIVANEDSRAARQEAERAEKDYELFLQELEEDQEMRHAINLYKDNNPPIHKSTGEDEMEEDEEEGAPGVDIDELLDELDDMTLDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBA3</accession>
    <name>A0A1E3PBA3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipase C/D domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_60746</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041896.1">
        <property type="nucleotide sequence ID" value="XM_019183052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBA3"/>
    <dbReference type="STRING" id="683960.A0A1E3PBA3"/>
    <dbReference type="GeneID" id="30200298"/>
    <dbReference type="OrthoDB" id="5317514at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004621">
        <property type="term" value="F:glycosylphosphatidylinositol phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029002">
        <property type="entry name" value="PLPC/GPLD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23221">
        <property type="entry name" value="GLYCOSYLPHOSPHATIDYLINOSITOL PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23221:SF7">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN-SPECIFIC PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00882">
        <property type="entry name" value="Zn_dep_PLPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133766" description="Phospholipase C/D domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase C/D" evidence="2">
        <location>
            <begin position="28"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="712" mass="78687" checksum="6AE7FB9BE03F9C4A" modified="2017-01-18" version="1">MKFPVKYLRALIALICFPTQVLSAGAAVHLSLASRVFQFLPDDIKDFPEYVLAGAFFPDAFYNCGNNPNASEYAHWPPFLAHGVEYYNDQYSDNSNSDQRKLKAFLYGIFTHQVTDSSWHSIRLEQGLLKMLAVMDFDQDINDAHSFLDTGGDFLHLLKLHDYSLLNTQWKYPMNDVLNIFERVGIPMSATMVKYCVSSGQAALLAEKTVLNRVGPVYAQSSPLLDDILENYYLGGLQEISSSIVKCLPNLGLWFKYGASTDPWSLCGAIISPSATSMNEIQVRDGTYLTPFTPLSNFGSSISIGNFINNELALAVGAPNEDQVGSVYVIPLSEIEANKMGTTMVLHGSSRVDFHLPKRFGSAVTSYKLLGQDFIIVTEPGTSNIYLYSGNIKLVTISDFTARSDYGSNGRKQEAISIEIFDFDGDSIPDIVVGSPLSDDSRVPQRGTIQILSGKLLSLILMNSRFGHEIDIHSIQFQEIHLPKLYQLKEGYEQFGAQTSVNKDYIFTSSNGLGIVFALSRSSGEVKHTIFKDKIDGLPIARQTSKESHRFGSNYIMNVEIEGVELLIVSAHRYTEGSCIGCGAVFVYTLVNSDIKFLTKLTLDISRKHMLSKFGFAALVSNGKLYISAPGYLTSGAIFRISLSEILHSHKEHVIVRSIAFQNQADEYTGFGEALAVSTDQGKLIVGSPNYGFQPPVHDNNKLTGNVIIYNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBI8</accession>
    <name>A0A1E3PBI8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">quinol--cytochrome-c reductase</fullName>
            <ecNumber evidence="3">7.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">WICANDRAFT_76496</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a quinol + 2 Fe(III)-[cytochrome c](out) = a quinone + 2 Fe(II)-[cytochrome c](out) + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11484"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="7.1.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </cofactor>
        <text evidence="17">Binds 1 heme c group covalently per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome c family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.8" evidence="3"/>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041529.1">
        <property type="nucleotide sequence ID" value="XM_019184396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBI8"/>
    <dbReference type="STRING" id="683960.A0A1E3PBI8"/>
    <dbReference type="GeneID" id="30201642"/>
    <dbReference type="OrthoDB" id="5925at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.760.10:FF:000002">
        <property type="entry name" value="Cytochrome c1, heme protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.100:FF:000003">
        <property type="entry name" value="Cytochrome c1, heme protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="entry name" value="Cytochrome c-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002326">
        <property type="entry name" value="Cyt_c1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021157">
        <property type="entry name" value="Cyt_c1_TM_anchor_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10266">
        <property type="entry name" value="CYTOCHROME C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10266:SF3">
        <property type="entry name" value="CYTOCHROME C1, HEME PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02167">
        <property type="entry name" value="Cytochrom_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00603">
        <property type="entry name" value="CYTOCHROMEC1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="Cytochrome c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81496">
        <property type="entry name" value="Cytochrome c1 subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase), transmembrane anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 17 18" id="KW-0349">Heme</keyword>
    <keyword evidence="13 17 18" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome c" evidence="19">
        <location>
            <begin position="86"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="17">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="17">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="17">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="17">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR602326-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ62322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="305" mass="33403" checksum="5B28C475963A317C" modified="2017-01-18" version="1">MFSSIRSTVRTNIKNFSTTAKQAAAEPTSLAKKFIAGGVAASGLTASYLLYEDSVTADAMTAAEHGLHPPAYNWSHGGMLDTFDHASIRRGYQVYREVCAACHSLDRVAWRTLVGVSHSNSEVRAMAEEFEYDDEPDDQGNPRKRPGKLADYIPGPYANEQAARAANQGALPPDLSLIVKARHGACDYIFALLTGYPDDPPAGVVLPPGSNYNPYFPGGSIAMGRVLFDDLVEFEDGTPATTSQMAKDVTTFLNWAAEPEHDERKRLGLKAVIVLSTLYLISVYVKKFKWAPIKTRKIRFTPPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBK1</accession>
    <name>A0A1E3PBK1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_103363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019041801.1">
        <property type="nucleotide sequence ID" value="XM_019180378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBK1"/>
    <dbReference type="GeneID" id="30197624"/>
    <dbReference type="OrthoDB" id="10672785at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="338" mass="39106" checksum="45687C04D9CEDD71" modified="2017-01-18" version="1">MPSMITANKDINDTLDERVGSTRLFTTEILSYCKNANEVSHIQLDLIHNKLQKKTVPELQKLKQDLIRSFSVAKTQIDHSKLYYNNILEDKDDLKKQLSEETISKKKEQLMKKLFTSKKKIEQLTKKKLEVREINRIIDSFSGIQELSYNEDQLKQIFGDQADAIKNAPGILKRASLVRVKKEQPDVKMEDKEEDEKKDEKKDDGSKKEEDNKVEEFETKEVIRYVFKTNLANSVEKLKEIQTQLLSSIEQYEKDIDRIKRSYIEDVQKFEAIKKILDPASVDQENGIDKEHHEDEDVEMGEAEGETPEAVDETVAEAGDPEDHHSEAESDIGDEAYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PBR7</accession>
    <name>A0A1E3PBR7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tropomyosin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_82767</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042052.1">
        <property type="nucleotide sequence ID" value="XM_019185436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PBR7"/>
    <dbReference type="STRING" id="683960.A0A1E3PBR7"/>
    <dbReference type="GeneID" id="30202682"/>
    <dbReference type="OrthoDB" id="128924at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000533">
        <property type="entry name" value="Tropomyosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19269">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00261">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="65"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="98"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ62845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="18978" checksum="6ACDEE62844698A5" modified="2017-01-18" version="1">MDKLKEKLANLKLDAEKWQEKHDDLQVKVKELEQENLEKDNQIQALTRKNETLEENLEKLEEQVTEHKTAAEESHSLKSHNENFTKKNQQLEEELEESDKNLKETTEKLRETDLKAEQLERKVTSLESEREDLEKKYEELSEKYAAAKAELDEISAQLEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PC86</accession>
    <name>A0A1E3PC86_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_61067</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ63026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042233.1">
        <property type="nucleotide sequence ID" value="XM_019183106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PC86"/>
    <dbReference type="STRING" id="683960.A0A1E3PC86"/>
    <dbReference type="GeneID" id="30200352"/>
    <dbReference type="OrthoDB" id="10255148at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1120.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1120.10">
        <property type="entry name" value="Ribosomal protein l15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024794">
        <property type="entry name" value="Rbsml_eL15_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000439">
        <property type="entry name" value="Ribosomal_eL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020925">
        <property type="entry name" value="Ribosomal_eL15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003269">
        <property type="entry name" value="PRK04243.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847">
        <property type="entry name" value="RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00827">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01384">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01194">
        <property type="entry name" value="RIBOSOMAL_L15E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ63026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="205" mass="24520" checksum="C611C230B4963492" modified="2017-01-18" version="1">MIVGAYKYLEELQRKKQSDVNRFLFRVRVWEYRQKNVIHRASRPSRPDKARRLGYKAKQGFVIYRVRVRRGNRKRPVPKGATYGKPTNQGVSQLKYQKSLRTTAEERVGRRAANLRVLNSYWINQDSTYKYFEVILVDPQHKAIRRDARYNWIAKPVHKHREARGLTAAGKKSRGLNKGHRYNNTKAGRRHTWKRQNTLSLWRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PCQ2</accession>
    <name>A0A1E3PCQ2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_89274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042191.1">
        <property type="nucleotide sequence ID" value="XM_019186178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PCQ2"/>
    <dbReference type="GeneID" id="30203424"/>
    <dbReference type="OrthoDB" id="10524563at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17316">
        <property type="entry name" value="Perilipin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ62984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="268" mass="30562" checksum="7265F39A39D00CE8" modified="2017-01-18" version="1" fragment="single">MADSIKTNNVHPQDINYLSNLIKQSSTLTHLNNYPLLNEISSFFLSFYIISYFATLVSKISLDFKNEILLKYFKFSIDWIDYFDKLFDIYILGNIDHFLPILGKIHLKDLYPSNIYNVTINEIKLYYQFSIAKIDEFKENQLKPTIISNTNPIIAPINSKLSTTIDNYLPSDNSIEPSPVSSSSSELSKFSNLLTLAYQRSKPLILGKFQNVKQYPTKIQSIYNDELNKNDQNITKAITNTSIEISNKIGLTNGKKEVKTNGSSNVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3PDE6</accession>
    <name>A0A1E3PDE6_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_60943</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454208">
        <property type="protein sequence ID" value="ODQ62897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019042104.1">
        <property type="nucleotide sequence ID" value="XM_019183084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3PDE6"/>
    <dbReference type="GeneID" id="30200330"/>
    <dbReference type="OrthoDB" id="10615660at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ62897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="332" mass="38044" checksum="A8DC551D99A029EF" modified="2017-01-18" version="1">MSRRLKTCLLKLTKSPSKKLPRPLLETIEKQKALEESFGFTQYPDPKVDAFMKKMNPIRKKQMITKLHPAGLYGMRAKEDFQRKKIHKGHNKETKLIKQAQRITLSPLRLQDELFQRYKGIRYNIYKKDLLENSVTLFVDDISDTASDVNYQNKIQTPILQLDASIQFKTVYSSIYKKVLKHDPILKDLVGKIEAPKILKPFGQERPTFKTIHIPNNSVNFAANAIEFLQKEKNAYKLFGVYVSLKKSPKISEELVQILQSFNDTSTPSTSTYDNLLKIAKESQSTANATTKRLLTTLGEADLLSVSIKDESITFTKPTSTAQEKNEIWKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU95</accession>
    <name>A0A1E3NU95_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_86346</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454216">
        <property type="protein sequence ID" value="ODQ56696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035903.1">
        <property type="nucleotide sequence ID" value="XM_019186036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU95"/>
    <dbReference type="STRING" id="683960.A0A1E3NU95"/>
    <dbReference type="GeneID" id="30203282"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF11">
        <property type="entry name" value="AMIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="5">
        <location>
            <begin position="73"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="3">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="228"/>
            <end position="231"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ56696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="549" mass="62081" checksum="76B4167141C96957" modified="2017-01-18" version="1">MKSWEELVESKKKFLNKQIPEEWILQEIKSPDEEPNVCVYLDKLLDEREIYITNLTLDELLSEQLSGKLSALEIAKAFTHRAALTHQITNCCTELFFDQAYQQAERLDKYLSETGKLIGPFHGIPVSLKDQLNVKGVATSIGYVHKLFNVVENEEDEAVLVKQLRLQGALLYMKTTVPMAMLLCDTFSGAYGYTQNALNRRYSPGGSSGGEGVVVKAKACILGIGTDIGGSLRAPAIYNGIWSIRPTANRFSYLNALNSWEHQDINPSTIGPMANRLEDLEYFGKHILGDDFQWTHDPKVPPIPWKVDVEIPKKLAFGFQKWNNIVMPHPPIVRMMEVVKKALIEQGHEIIEYEPPISDMELFEFSDKVFGVDGYLELDREVANSGEPVLMDELYSSTGSRPKPITSIEPYWELAGQKYRYQQTFAKYWLSTKEKTSTGRPIDALLTPAWNCCATRLGKVPKINADYAVQYNVLDLPAIPIPVGTVDSKIDPKDPNYKPVNHFDQDCYDRYDAEFFDGANISVQIVGLQKFTEERLLKLAGVVRDSIKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUP6</accession>
    <name>A0A1E3NUP6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mto2p-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_86248</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454215">
        <property type="protein sequence ID" value="ODQ56804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036011.1">
        <property type="nucleotide sequence ID" value="XM_019186022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUP6"/>
    <dbReference type="STRING" id="683960.A0A1E3NUP6"/>
    <dbReference type="GeneID" id="30203268"/>
    <dbReference type="OrthoDB" id="3980439at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="257"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="544"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="857"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="176"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ56804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="950" mass="108690" checksum="EE903771C0C644D1" modified="2017-01-18" version="1">MASVQRQQQQRLEDHNETLESISQFNNLSLDESVDEDQGMNGGDDVGESPSEEMGNKSDTTDSSINIKNSSYVESNNNSNGEETNEQKNLENEDSEDGVDDGSDNDDSANEFLNSSSFLSNSQIIRSANLNSYKPINQKTISSPTPALKSYTPKSKDHFQFSGSKSAPPGLSDHSDAEEEDSDDKEDEDDVDLHNGHYSHSKQNNIDIDDPESTPKLPKINIDTTPWRKLRPPSALPSKEVPTQQLSLNFMKSLFDGDNENDPIRSPTRSVSLFESSGNTDSLGTTTATTSYEVENLKKQITNYKLQLRLLTEFIRNIIRNTGNGSGSIKNDIMERLERENDILFNERERLQKDLKDTIGFHETEESAKVSEKMKEILELKNDLIATRDLNSELNSYIDELKARTEDQDHLIETSNEDADRWEHLINDILTLLLDFLKGESAKAVLQARDQSKSLDTKLKVVSFSINEILENYEHLSDNISKANGYDAESISTFIEQSQTKIANSKKIEQDLKQLLKQQSDSFTSLKSEFSSLQNQFKENLEFVASLRKLLGEKREKIKKLNDTIIQLEAQVEEHGSNPHESSTGIEKETQLLRNRLTIIKTNHSKEVENLHAEIENMKLKIDEISAKGPTGDVILTEELLKKQRAIGELDNEINALRQQLETKDDQVDSTISNLKNLLRQTKRDYESLLQEYHHIQNLLKESQSELQSKDKEYRRKLEHAAKELNLAVAKQRSLAAEKSKLTFALEDSKREHSLLKLNNSTLTEKVGKLSYQVSSLTNYELGFNSLFQLQITNFQNLLHHFDSILEESSVNQAQTKLSKLVDSELDFDQIHGIMRSLFSYFENATHSLISDHSQLLINSNIEFKNSQNEINSLKNQIDSLNEELQWYIVNYRDQNDLSPRSKLRIDELEKRRKAERERRKLENQASEERILKLEKENKELKEKLKQINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVD1</accession>
    <name>A0A1E3NVD1_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_98310</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ56652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019035859.1">
        <property type="nucleotide sequence ID" value="XM_019186647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVD1"/>
    <dbReference type="STRING" id="683960.A0A1E3NVD1"/>
    <dbReference type="GeneID" id="30203893"/>
    <dbReference type="OrthoDB" id="4091755at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ56652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="51" mass="5680" checksum="8D661C20FAB5F708" modified="2017-01-18" version="1">MLTGDHPGTARTIPQDIGILPHNLYHYSQDVVDSMDQAVEVSRDYPVVITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVL7</accession>
    <name>A0A1E3NVL7_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_65436</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036383.1">
        <property type="nucleotide sequence ID" value="XM_019183910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVL7"/>
    <dbReference type="STRING" id="683960.A0A1E3NVL7"/>
    <dbReference type="GeneID" id="30201156"/>
    <dbReference type="OrthoDB" id="10265275at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045237">
        <property type="entry name" value="COPS7/eIF3m"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350:SF5">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7/DENDRITIC CELL PROTEIN GA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0736">Signalosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="187"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="187"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="200"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="26666" checksum="DEBC9FEE2B563205" modified="2017-01-18" version="1">MSKVEVHDRILAALSDKSTFGFNELRLDKSVNDVLGDDVKIKNTLELFSFGSIKDYNDHKNDFIELSSPALFKLTQLTLISEVLINNEFSYNYLKSVLNIDSTPELERLITESIYSRIIDAKLYNEQGIVQVYSSIGRDVLLNNETEKKYGSNKRVSDLIQGLRSFGAKVEKGQKYIDEVQSSITKEEMTTTTSLNNTYSGEETTPTPSGSSPTKSKETKEQQALNNKRKRKIEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVT4</accession>
    <name>A0A1E3NVT4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WIBG Mago-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_65539</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036488.1">
        <property type="nucleotide sequence ID" value="XM_019183930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVT4"/>
    <dbReference type="STRING" id="683960.A0A1E3NVT4"/>
    <dbReference type="GeneID" id="30201176"/>
    <dbReference type="OrthoDB" id="21625at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903259">
        <property type="term" value="P:exon-exon junction complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039333">
        <property type="entry name" value="PYM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015362">
        <property type="entry name" value="WIBG_mago-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036348">
        <property type="entry name" value="WIBG_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22959:SF0">
        <property type="entry name" value="PARTNER OF Y14 AND MAGO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22959">
        <property type="entry name" value="PYM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09282">
        <property type="entry name" value="Mago-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01273">
        <property type="entry name" value="Mago-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101931">
        <property type="entry name" value="Pym (Within the bgcn gene intron protein, WIBG), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WIBG Mago-binding" evidence="2">
        <location>
            <begin position="18"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="143"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="177" mass="19919" checksum="8BA9B0C2A9433BC3" modified="2017-01-18" version="1">MGGANRESVSGIHADENGDRVIGGSVRADGTVRKTVKVRPGFIPKEDVKRYDVRERIRQRQEERETKNDEKVDEVRKPGGRPNRQFGVINNILQSNSKPQQPKKDEKVQHDSNKSNNETTELESPFDKLSLGSKESSPQPSNKSTTSTKESSTSKPKKYVPPSRRKKYTLADLDNSQ</sequence>
</entry>
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    <accession>A0A1E3NVV0</accession>
    <name>A0A1E3NVV0_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">WICANDRAFT_15115</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036327.1">
        <property type="nucleotide sequence ID" value="XM_019180633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVV0"/>
    <dbReference type="STRING" id="683960.A0A1E3NVV0"/>
    <dbReference type="GeneID" id="30197879"/>
    <dbReference type="OrthoDB" id="10062838at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026505">
        <property type="entry name" value="Solute_c_fam_35_mem_F3/F4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19346">
        <property type="entry name" value="SUGAR PHOSPHATE TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19346:SF4">
        <property type="entry name" value="SUGAR PHOSPHATE TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133548" evidence="2">
        <location>
            <begin position="23"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="383" mass="43071" checksum="0D931A273E0381BD" modified="2017-01-18" version="1" fragment="single">SNKKLFAVVSLFLISLFSFVSQTELTSYLYNNLDFDQPYLLLYLTHGSWWAIWPIQVLSIAIFKHFKKSRKYKYNVLDFKRFKRNISYSLKNQHKNIFKTSGIVINENYQHDHEYPNSILTFILSKSIKSIATMIFIVTMILSIAGCTWYVAMGIAPASDITAIYNCSAFSALVFAIPILHERFSYLKLSSVALAIIGVFFVAYSGESGSSEKDFPYRVIGDIIIAIGAILYGLYEVLYKKLCCPPINSVSSRRQVAFSNFCASLIGLTTICTLWIIIIIAHVFGISKFKLPTSGYMWVIIISSLLSNLIYSLSFLALMSLTSPVLSSVSSLITILFVGLFEWILFGVKLSAGQLIGDLFVVIGFGVLSYSYWAEITEEDVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVX7</accession>
    <name>A0A1E3NVX7_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_107603</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036493.1">
        <property type="nucleotide sequence ID" value="XM_019180508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVX7"/>
    <dbReference type="GeneID" id="30197754"/>
    <dbReference type="OrthoDB" id="5364312at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="414" mass="46336" checksum="6F141F0FF114E97F" modified="2017-01-18" version="1">MSQIWNFLNKESSNQSENDNVNDMDQFTNISKEDGHVDYFNHHLQNEHSSNSDSTLEGDDTHNHNINSSNSDHETFNSQLKRRLSKLTNHKKQQQQQHDQQTTPKKNYKKHDISCLKKDENSQSSCDCLIDEQDDQVNEIVSPIIIRSSTTHLPNIDSNVLSPNSLSTTSSLIFERNVQDQITNYCNCTTTTVTSTSTNRSRRNSHNLLARSQSVSSQSSIPNHLNFEFFIPQVLDATTEVLTDPNYNENVEVIQSSNNSNLSSSLLADSTNPQSSTRPGLGSRRMSRSVPISRRQSSCAPLSPISTTTTTTNINDVDYSDKKKLNFCSFADLLSDETSSSDVLISPTSPKNNSTLGMTSNSTFQDLRDPFNKDHVFTQPPPPLQTQSNPPFPSLSKTTSIKEILLENQSELGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NW40</accession>
    <name>A0A1E3NW40_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_36592</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019182453.1"/>
    </dbReference>
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    <dbReference type="STRING" id="683960.A0A1E3NW40"/>
    <dbReference type="GeneID" id="30199699"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF33">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="132"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ57333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="689" mass="77897" checksum="3A31A717C4E98872" modified="2017-01-18" version="1">MSSKIQALDSLNEKYPAPQRFQAEPIKGSSQDGFTPVYRNSAIGQNGSLIDSIHPDLDDLDKLFQNAVKTFPNENCLGYRKFNNIIKNKYDNHYTYETYKEVDLRKNNIASGIIYFVTRHPNYNKVLNDKGKPDFVVSILSPNRKKWVLLDIASRAFSLPTTALYPTLGEESSKYILELTKSPIIFTVKSKVEEILQLKQKGLPNLNIVVSLDDFNYDDHSLFDEAKKLNLTLVDFRAIEKTGERNLLSTSFNPPSPDTIYSICFTSGTTGLPKGVVLTHRNAVAGVFKVNKADNGEQLRAISALPFAHIYERQMMNFELSSGYALAMPTLTKDPSFLFDDIKACKPHWIANVPRVYNKLEAAIKVFVQEHFKVDALGENPNAPIIDKMILRNTVREAFGFQNLKYFVTGSAPLGTDTINFLKDILCCGGMTAYGSTESFAGVCFGDPFETNTTNSSGPPGVAVEFKLRNVQQMGYSANDKPLARGELLLRGPQIFKEYYKNPKATKEAFDQEGWFYTGDIAAMDSTGRVYIIDRLKNFFKLSQGEYVTPEKIENIYLANSPLITQVFVHGDSLKNYLVGVAGIKSESIIELLKQHHNIDVTEPELKEFLKDPKFKRTIVQELNRHVKDCDLQGFEKIHNLHFDFEPLKVEDEVLTPTLKLKRNNARKKFEDILKKLYDEGSLVKNSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWK2</accession>
    <name>A0A1E3NWK2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">WICANDRAFT_96817</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036700.1">
        <property type="nucleotide sequence ID" value="XM_019186540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWK2"/>
    <dbReference type="STRING" id="683960.A0A1E3NWK2"/>
    <dbReference type="GeneID" id="30203786"/>
    <dbReference type="OrthoDB" id="942095at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036464">
        <property type="term" value="C:cytoplasmic ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000650">
        <property type="entry name" value="Serine/threonine-protein kinase ppk16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="37"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="394"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="446"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="687"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="394"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="449"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="480"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="538"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="589"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="612"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11 13">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="157"/>
            <end position="158"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="12">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ57493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="712" mass="81117" checksum="3AE691B9DFD5AFE1" modified="2017-01-18" version="1">MMNGMRNSYNEKAKIAESYNKLYSQFSSDEVKEVGNYSIIKLIGEGAFGKVYLANHRLTHTKVVLKTGDKTDPNLVREVYYHRQFKHPHITRLYEVVVCEGKIWMVLEYCPGRELYDYVLSCGRVPIDEVKKLFAQIASAVFYAHSLNCIHRDLKLENILLDKRRNAKLSDFGFTRECQQRNFLETVCGTTVYMAPELIQKQKYDGFKVDIWSLGIILYTMLYGQMPFDDEDEIRTGYKIVNEEPPYREDIPQEAITLMRRLLSKVPSERPSCLEILNDPFLENIGLSLLESSMTLLKNIVGQKNFHSKSEKHLLKKLKSLGIDTHALQKSVVSKKADSLCGLWELLLEKEKKKEFKKYKTRSRSVLRISESTSRRVSQLMDPNNSPQLSRIVSGFSNKSEPTQENQINYGDGNSMDKSTSASIASPEKKKNGFIQKFTKLWKTKKSDNTGAVSNQKNSFDSIQKDSKSHHQYNINNNITTTPSQQQQSAQQSTSKLSIPKPTFKTSPKKLTIHEPKSPTRVVNGAPETRSRPRPVSMISQQSLVSQVTTMSDMSAASHNTTNSDNTTRPAFTRKRSSDTSLHSKNTTRRSLSLISSNSSASDRSSRKGSFYDQTTSSDYTFRTNRGRKYNEAIFPKSFKRRKSPLGGIPPSSKFKRSKAFIIEEEEDDEDVDVDDDNMEDIETDLENLGIAESTRNASDFESDVSERGRQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWN9</accession>
    <name>A0A1E3NWN9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Micro-fibrillar-associated protein 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_71058</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036309.1">
        <property type="nucleotide sequence ID" value="XM_019184231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWN9"/>
    <dbReference type="STRING" id="683960.A0A1E3NWN9"/>
    <dbReference type="GeneID" id="30201477"/>
    <dbReference type="OrthoDB" id="1111734at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033194">
        <property type="entry name" value="MFAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009730">
        <property type="entry name" value="MFAP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15327">
        <property type="entry name" value="MICROFIBRIL-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06991">
        <property type="entry name" value="MFAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Micro-fibrillar-associated protein 1 C-terminal" evidence="2">
        <location>
            <begin position="95"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="277" mass="32791" checksum="136B1468647FA1EA" modified="2017-01-18" version="1">MAVKRHFTGKPIGQEYEPSDDSELESEVEEDENEKQQQQKSIKTNIQIRPVERDVIQKDEEKVEQPLEELQAEQENQSESEEEEESSDEEDSSEEESSDDEPKMLKPVFLSKTKRAQVIKSSTPPKTKDQKAAEDRERTLKTIEAHINQDIEYRKQLKESTQNYGGIDDTDDLDPEAERTEWELRQLQREQRDRAKLEQEQDELEELENRRLRTEEEREKEFREKQATEEQNEDGSSKSKAKGAFFRDEELLKRDLGEENEKYDKNLLPQRFNPDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWT9</accession>
    <name>A0A1E3NWT9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 19</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED19</name>
        <name evidence="11" type="ORF">WICANDRAFT_97023</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 19 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWT9"/>
    <dbReference type="STRING" id="683960.A0A1E3NWT9"/>
    <dbReference type="OrthoDB" id="2160599at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013942">
        <property type="entry name" value="Mediator_Med19_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28270">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28270:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08633">
        <property type="entry name" value="Rox3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="128"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="143"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ57631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="156" mass="17632" checksum="45C67744A9864A8E" modified="2017-01-18" version="1">MSDVNQTNPYYYYVDPNVQYTKSKPHPTENLIDLYNLSGIANSVARLNEDGTKGVKLRKSYKAHINDLLGKHVILPKDRTISPIVFAPEREGAPIQIKKIDEQFLRSYLNFDKTSEDGIPGFDPVNLALGGEVGQGTKRKQKKGNEEDTKRRKLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWU9</accession>
    <name>A0A1E3NWU9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_13409</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036379.1">
        <property type="nucleotide sequence ID" value="XM_019180571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWU9"/>
    <dbReference type="STRING" id="683960.A0A1E3NWU9"/>
    <dbReference type="GeneID" id="30197817"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043813">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043573">
        <property type="entry name" value="Fig4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738:SF5">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="4">
        <location>
            <begin position="143"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="679" mass="79765" checksum="ED9EC5F569A2FA2E" modified="2017-01-18" version="1" fragment="single">KSRKLILQKYTIYETSTRVYIVGSNGRETMFRVLEIDLTTSKEELSIVEDNVFFTRKEIMNVLSRLEETNAEGLTKKLTGFGLLGFIRFTDCYYLCVVTKRSPIAILGGHYLYHIDDTQLIPISNNYKKPDNYSEEARFLATFANLDLSKTFYFSYTYDLTNTLQRNILREKKKAYGFPNTEKFHDQYNEMFIWNSSLLKPIVETYDRLYDWFQPIIHGFIDQANVSIFDKKVYITVIARRSHHFAGARFLKRGVNNQGNVANEVETEQIVSDLLTTSFHDTGCGFYNNPRYTSYVQHRGSIPLFWSQEGTNLKLAKPPIEIDIIDPFFSSAALHFDDLFKRYDSPVLILNLIKQKERNPRETKLLKEFEECIRYLNQFLPEESKLKYTAWDMSRASKSDGQDVIEFLETYADETLKTTSFFHNGPSLDTTKLQQGICRTNCIDCLDRTNAAQFVIGKRALGYQLHALGIIDDVFIDYDSDVVNILTEMFHDHGDTIALQYGGSHLVNTMETYRKINQWSSHSRDMIESIKRFYSNSFVDAQRQEAINLFLGNYVWEKGKPMLWDLNTDYYLHNIQSLSKQKRSYRYWWTESNLIKFRDAYDVPKDGLVLKKLEPYQGFFDNYWNEYYVPRRLTLFEKLFEINMNSTLRYIAPIENLEDAFIISPFKSRKQTSINHKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWV3</accession>
    <name>A0A1E3NWV3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein translocase SEC61 complex gamma subunit, archaeal and eukaryotic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">WICANDRAFT_35505</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036876.1">
        <property type="nucleotide sequence ID" value="XM_019182314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWV3"/>
    <dbReference type="STRING" id="683960.A0A1E3NWV3"/>
    <dbReference type="GeneID" id="30199560"/>
    <dbReference type="OrthoDB" id="2401875at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005784">
        <property type="term" value="C:Sec61 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071261">
        <property type="term" value="C:Ssh1 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12309">
        <property type="entry name" value="SEC61 GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01067">
        <property type="entry name" value="SECE_SEC61G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ57669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="68" mass="7634" checksum="4B8EA1CC19849E87" modified="2017-01-18" version="1">MAGDGLEKFTEVPVEFFKDGSAFVQKCTKPDKKEYIKIIRAVGVGFIMMGIVGYAIKLIHIPIRYLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NWZ2</accession>
    <name>A0A1E3NWZ2_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">WICANDRAFT_35485</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat rae1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036812.1">
        <property type="nucleotide sequence ID" value="XM_019182310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NWZ2"/>
    <dbReference type="STRING" id="683960.A0A1E3NWZ2"/>
    <dbReference type="GeneID" id="30199556"/>
    <dbReference type="OrthoDB" id="256303at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051664">
        <property type="term" value="P:nuclear pore localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000728">
        <property type="term" value="P:regulation of mRNA export from nucleus in response to heat stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032185">
        <property type="term" value="P:septin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000190">
        <property type="entry name" value="Nuclear pore complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="74"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="115"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ57605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="361" mass="39917" checksum="1290657F3690B617" modified="2017-01-18" version="1">MSFFGKPAASTATSTSQATEADLANDIIVQNPPEDSISDIAFSPQAEFFSASSWDKKVRIYEVSPTGSTEGRALYEHDAPVLTTRWTLDGTKVISGGADKQVKLYDIQSGQAQTIGMHDGPVKAVRFVECGPTNTQVVVSGSWDKTLRYWDLRQPQPVSTIQLPERVYAMDASQKLLVVGTAERHVCIIDLNNPQQISKQAMSPLKWQTRSIACYPQANGYALGSIEGRCAFQYIDDQEQAKSGFTFKCHRQNETKAGRTESHIFALNSIAAHPVYGTFATAGSDGCFHFWDKDAKHRLKGFPSLGGTISAAAFNRNGSIYAIAVSYDWSKGYSFNNPQYPNLIRLHPTKDEEVKQKNKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX41</accession>
    <name>A0A1E3NX41_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_85818</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036925.1">
        <property type="nucleotide sequence ID" value="XM_019185952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NX41"/>
    <dbReference type="STRING" id="683960.A0A1E3NX41"/>
    <dbReference type="GeneID" id="30203198"/>
    <dbReference type="OrthoDB" id="1935484at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF32">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="403"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="433"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="466"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="66"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="499"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="504"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="518" mass="57874" checksum="95493A2BAFB433B2" modified="2017-01-18" version="1">MTAVLESVADNKAQWESTVETTSSHFDETGEQKYKIILPNGEELKVIKDWTDKEEARVRLKLDFLILPILAGCFFTLQLDRSNLGNALTSTLRKDLGLTSYQINVGNQLLYIGIVLCEIPSNYVLQKIGPTVWLSFQVLAWGLIALSQAFMKNYSQYLALRILLGIGESGFIPGSLYTLSKFYKRNELARRHIYFFLGNILASCLGGFIAGGIIKDLTGKNGWPGWKWLFFVEGLATIGAGLILGAFLPMSPEKTSSLLFPKFDLFSDRERLILRSRILLDDPVKALDIQKKITLRDIGNAVSKWRIWLHFLISATAIGPIIPIGTYLPTIIKNMGYTQFQANAMSTISSWICAVFLVSSAFTQDKYKPRAAALLVFSGLQSVFGIVLRAITTRHSNKLRFSMITVFSCFTSVCHLLNTSWVSVNATSPTERAVFLAIVIMAANCGGVYGAQIFQDNDAPYYPRGFLAILLLSIVLLLLVLLAFFQYKLANKKLESKYGPVQKAESRDQEKPPERQHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NX94</accession>
    <name>A0A1E3NX94_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_65114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="30201104"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133547" description="Secreted protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ57768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="19261" checksum="11DA4184A1C7A128" modified="2017-01-18" version="1">MKFSTALIASSLAILAYAKPIADALPWAQANPQAAAAAQAYADAYAEAIAIAHPDPEAYALAASADDCADVTCHMNCGMMIIAGQACSENENDNYSGPYTPGCLCDDAKDTNFQKYYDACMNCGWTLWKYYSPYLEPALKDCHASYPSISTEPTGTSRCSTTLTNSYTKETDINYSSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXK0</accession>
    <name>A0A1E3NXK0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamine amidotransferase type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_97237</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454213">
        <property type="protein sequence ID" value="ODQ57825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019186578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXK0"/>
    <dbReference type="STRING" id="683960.A0A1E3NXK0"/>
    <dbReference type="GeneID" id="30203824"/>
    <dbReference type="OrthoDB" id="14446at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061672">
        <property type="term" value="C:glutathione hydrolase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01908">
        <property type="entry name" value="YafJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026869">
        <property type="entry name" value="EgtC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052373">
        <property type="entry name" value="Gamma-glu_amide_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43187">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE DUG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43187:SF1">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE DUG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13230">
        <property type="entry name" value="GATase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="305" mass="34285" checksum="70EE876C5E996790" modified="2017-01-18" version="1">MNGDGFGVGYYSLDPELEDGPCVFRAITPAWNNMNLERLAEKTKSNLVFAHVRASTYGVLSETNCHPFTYHSLMFMHNGGISGFDKIKRKLVAHMKDEYFIFLQGSTDSECCFALFLDTLDKMGFNPADESTNFGHDVLREALLQTIEYIKNWTKDITSEPSLLNFAVTDGHSVVVSRYITSKTEEAASLHFSSGSSFVEYEPGQYRMERLDRGQNVIMVASEPLTFERGDWIAVPTNSILSIKNQTVLLHPIKDEYYQEDPLYSRSSNFAESKGLMGSVPLADTVDVEIPPLEREGRSRPLTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXL4</accession>
    <name>A0A1E3NXL4_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_35918</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454214">
        <property type="protein sequence ID" value="ODQ57287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019036494.1">
        <property type="nucleotide sequence ID" value="XM_019182373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXL4"/>
    <dbReference type="STRING" id="683960.A0A1E3NXL4"/>
    <dbReference type="GeneID" id="30199619"/>
    <dbReference type="OrthoDB" id="2537459at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133551" description="WSC domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="2">
        <location>
            <begin position="18"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ57287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="101" mass="11168" checksum="632B07F2C78A5A37" modified="2017-01-18" version="1" fragment="single">MILIQNFITFILIIRLTSATLSYCSSQNTGSQLVNSSIYQSNGLCHDKCNQEGFALAIVQYQDCWCSNYVPGNTVDVDECNMNCPGFPSEKCGGDDLYGYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXS3</accession>
    <name>A0A1E3NXS3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_94966</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 81 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037112.1">
        <property type="nucleotide sequence ID" value="XM_019186473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXS3"/>
    <dbReference type="STRING" id="683960.A0A1E3NXS3"/>
    <dbReference type="GeneID" id="30203719"/>
    <dbReference type="OrthoDB" id="4473401at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052861">
        <property type="term" value="F:endo-1,3(4)-beta-glucanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.420:FF:000008">
        <property type="entry name" value="Endo-1,3-beta-glucanase Engl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1170">
        <property type="entry name" value="glycoside hydrolase family 81 endo-[beta] glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005200">
        <property type="entry name" value="Endo-beta-glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040720">
        <property type="entry name" value="GH81_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983">
        <property type="entry name" value="ENDO-1,3(4)-BETA-GLUCANASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983:SF20">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17652">
        <property type="entry name" value="Glyco_hydro81C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52008">
        <property type="entry name" value="GH81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 81 C-terminal" evidence="9">
        <location>
            <begin position="7"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ57905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="368" mass="40896" checksum="F3E4E11EA651CFD8" modified="2017-01-18" version="1">MSKDLSYTTEQLQLLASVANDDLAVDFTSSTKGLTTYYAGKYLDKYAYILLVIDSIIQDESVRNSTLERLKDAFDTFLDNEQLFPLMYDTRYGGVTSTAAQNGDTGADFGGSYYNDHHFHYGYFIHAAAVIGMVDAKLGGSWAQDHKDWVNSLVRDVANPSKSDTHFPVSRMFDWYAGHSWAAGLFAAADGKNEESTSEDYNFAYGMKLWGKVIGDSSMEARGDLMISIMAKSMNDYFLYSDDNDVEPEQIIGNKVSGILFDNKIDHTTWFGTNTEYIHGIHMLPVTPISSVVRGPTFVSQEWSQVISPIVDSINSGWTGILRLNQALTDPKSSYNFFSQDNFSSAWLDNGLSRTWCLAFSGGLANAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXT2</accession>
    <name>A0A1E3NXT2_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_106383</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037175.1">
        <property type="nucleotide sequence ID" value="XM_019180473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXT2"/>
    <dbReference type="STRING" id="683960.A0A1E3NXT2"/>
    <dbReference type="GeneID" id="30197719"/>
    <dbReference type="OrthoDB" id="4159781at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF12">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="33"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="102"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="203"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="265"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="336"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="828"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="960"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="268"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="353"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="837"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="977"/>
            <end position="988"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ57968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="1096" mass="123844" checksum="617D8F1CAA5B5FED" modified="2017-01-18" version="1">MVAASKANSNKVVKKDKKTAAAANKRRNRVPISCTICRRRKVKCDKKRPVCTACHRTGVAHLCHYIDPPWAQPLQNSEINPPQEPESEVERLKEKIRQLESQLFPNEDHLPSPNEEADDHESNSSHEHNISTDSLPNEALNLGKKFDMLHIKSTTTVHLGATSWLAIMKGDPYLRVLWAHIFKMRKKVEEMKFKDKMIRHQQEQEQVREGKKLSTSQVCPVSGKDNSTGGQCPVSHSLNYNESVTESSSCPVMSTASISISKCPVSHVRSETMTREVRKAEDEGDDETMDENNTPETETSSDHNNKLHEKVCPLMIGDTSFMDDFNAHNASSCPVIKDKKTKKRRRSSSKSESSGKTEPKLSDKEAALQYAQNPVMMLEKYLPKKKVLWLHIKRFFEVLYVYMPYVDEPSFIEELKYIFGEEDDEDQTIKIKIRSGVDFALVGTVCIIARLSWHTLPMNNSAKLLKKSSNHSKNYQEDQSIIQFLQKPENEIPLKLVDCVKQCFSNLKLMRKSSLKIVQCALYMRLYFMYSPDDGEGADGGDSQIFLGMIIQMAISIGLHRDPKNFENFSNERQRHLWRKIWFKLISLDVTQSMNLGCPRALQNHIEFSDTELPGEVDDDGSNLEEFKNDLRELTIINNIHTQAKIDDLIAKTMKVLLNVNKPARRFQVDQLIDQLEQSISGFGVITDKYALPQIGKLLVNRSKNEPLYVSGARAIEFRLHVTVNMLLYLLNYILYVHFEPRGSQDQQVALIAKNYAQKALNCALEGYRNCTLFFDCGGDYFGPGSDIVLAPLLLLVGHRSMQFMVSLILRSRCGPFMKPSVVEKSSFPMVPDDSNDSSQTGSNNIDNRNYDLNSVEIKDESEQYVSAFNVDVNSGEALATILLSHMDSFHKLAETLGQKYSYSWRMGKAVGFFVTLLKKPVNIVKDLVKTENLKDVEADKGVSNVLNAVPLVLSTDEEKKAKTKPLTSKLDEKPQGQESQPTQSNFNQSNLFNPFFENLEANLINPNLDDATMVDQFKNDLQQSENFLSFKDPSQSMGETFGFTGLPDLDLLQGSSSLDNFMTGLQEDKDGLNSMGDGAGSAVDFNNMMSSSWFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NXT3</accession>
    <name>A0A1E3NXT3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_23213</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ57915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037122.1">
        <property type="nucleotide sequence ID" value="XM_019180981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NXT3"/>
    <dbReference type="STRING" id="683960.A0A1E3NXT3"/>
    <dbReference type="GeneID" id="30198227"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000007">
        <property type="entry name" value="Nitrogen regulatory AreA, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF281">
        <property type="entry name" value="BOX A-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ57915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="50" mass="5713" checksum="F4CDBA755F690E98" modified="2017-01-18" version="1" fragment="single">CKNCKTSTTPLWRRDETGQVLCNACGLFLKLHGRPRPISLKTDVIKSRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NY50</accession>
    <name>A0A1E3NY50_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein transport protein SFT2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_34815</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Nonessential protein required for the fusion of transport vesicles derived from the endocytic pathway with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Golgi apparatus membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the SFT2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037303.1">
        <property type="nucleotide sequence ID" value="XM_019182235.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NY50"/>
    <dbReference type="STRING" id="683960.A0A1E3NY50"/>
    <dbReference type="GeneID" id="30199481"/>
    <dbReference type="OrthoDB" id="660759at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF36">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SFT2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ58096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="146" mass="16266" checksum="580CF1EFF6F73888" modified="2017-01-18" version="1" fragment="single">QGLFEFSRWDRLIIFAVAMAGALSCWIVCFLLFPVLSLKPRKFAILWSLGSILFLFSFGALHGAKLYLIHLFSIDRLWFTISFTASIVVTLVSSLVLHSTILAIIACIVQLICSVAYTISYFPFGKNGLRLASNVAIVQVDSWINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NYB2</accession>
    <name>A0A1E3NYB2_WICAA</name>
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        <recommendedName>
            <fullName evidence="3">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_80305</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ58148.1"/>
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    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="30202281"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="567" mass="66266" checksum="76F7A8F1C0A0DC2E" modified="2017-01-18" version="1">MNKEIQLLLLDEVTTNNEKMNQIYELLKISSEIIEQFEEQGLEHDNLLIIHYRNAREILKILDRSDYLEFYIKLLEFGCSTASSNSINEIRHALSHGEPHEQEVFEFAAIFTHADKETVDFLKRKMLTFYSFDMIKQIVVKYIQKGEPEKVEFYMEYLLTKYALDKDKLGHHDQKILTSKLYEVLTYHAVRLKNYESAIGLLETVKNNGIEISPKLLHILATSFRQKKMFDAVLVVLRKAAEMMEYQNVGNFYKNMIIGELLITLRAKFPDNPKVLISQFISIFPGSEIMLNQLGLLSLIYNGTVDEIGNLNNNLPVSKSEVSDFIKITDGYPTIENMTELYITVLNHIGLHKHKYSSEFVFNELFNKFKDYVVQVQEANIPNHPFSKDKMDEGIVNVFVRRTIEDLKNPSLATAFLKSFLQTCDFRRFDGRAASMILYFNRDNAEHYELDELITIMGKLHAPLGFYAITAMILRNDKRMNYEEAYEWYKKLLTLGKSITHFDLIQVIKKHGWELPEDFDHSLLKLQYMNLQDRREIEFEQDNDEVVINDPEFVQNLSATLDEVVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZ90</accession>
    <name>A0A1E3NZ90_WICAA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_96122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZ90"/>
    <dbReference type="GeneID" id="30203758"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="88" mass="10161" checksum="949DB2F015223B81" modified="2017-01-18" version="1">MPFVRRSTASPATTTRPTEEAITLQSKWRVESTEIELKLCLRIRIAIECTLSQVLICYSPFNSRKYLFVAAQLEETTSSNYNMSYNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZE8</accession>
    <name>A0A1E3NZE8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Altered inheritance of mitochondria protein 32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_15670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIM32 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037601.1">
        <property type="nucleotide sequence ID" value="XM_019180652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZE8"/>
    <dbReference type="STRING" id="683960.A0A1E3NZE8"/>
    <dbReference type="GeneID" id="30197898"/>
    <dbReference type="OrthoDB" id="10253744at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03062">
        <property type="entry name" value="TRX_Fd_Sucrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009737">
        <property type="entry name" value="Aim32/Apd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902">
        <property type="entry name" value="ACTIN PATCHES DISTAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902:SF7">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06999">
        <property type="entry name" value="Suc_Fer-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="216" mass="24143" checksum="CF41A41CAA344F0C" modified="2017-01-18" version="1" fragment="single">PESHPINHDKPLLNTKSPTYKHVLIPTDVPATEWPSKVELVPGSLISEFTSLKRESLDPMYPVMISNIQVQDPQGDVLVFPDNEWHDVPYVSKFMTGNLTPNGIPQKGIQNKNQYVFICGHAQRDIRCGLIAPELAKEFEHVLRHENLLYDKATNPEGVKVGIVSHVGGHAYAGNVLYFDKEGLGIWYGRVEVGHVDPIVKETILGSSILQELYRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NZU9</accession>
    <name>A0A1E3NZU9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF985 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_31727</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037815.1">
        <property type="nucleotide sequence ID" value="XM_019181873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NZU9"/>
    <dbReference type="GeneID" id="30199119"/>
    <dbReference type="OrthoDB" id="6614653at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072527">
        <property type="term" value="P:pyrimidine-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06121">
        <property type="entry name" value="cupin_YML079wp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009327">
        <property type="entry name" value="Cupin_DUF985"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039935">
        <property type="entry name" value="YML079W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33387:SF3">
        <property type="entry name" value="DUF985 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33387">
        <property type="entry name" value="RMLC-LIKE JELLY ROLL FOLD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06172">
        <property type="entry name" value="Cupin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF985" evidence="1">
        <location>
            <begin position="37"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="224" mass="25464" checksum="0286AD2E35759638" modified="2017-01-18" version="1">MPDQSLQSPTIDGLHNVIVKKPLYFTQTFGKSESNDQFINELGLEGHREGGYFKETDRSPFLLENPFHKDDNKPIKPINDNQQLAKDKYRNYSTLIYYLLTPEIPYGRFHKNNNRIIHILQKGRGQYVLIYPDGSVKSFIVGFNHSKGEVSQWVVPGGVYKASFLLPLNESTGESKDDHLLISEVVVPGFEYDDHEFLEGVDTLAGMVGESKAKELSFLLHESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P026</accession>
    <name>A0A1E3P026_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS11m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_64431</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454212">
        <property type="protein sequence ID" value="ODQ58292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037499.1">
        <property type="nucleotide sequence ID" value="XM_019183735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P026"/>
    <dbReference type="STRING" id="683960.A0A1E3P026"/>
    <dbReference type="GeneID" id="30200981"/>
    <dbReference type="OrthoDB" id="1654884at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000011">
        <property type="entry name" value="37S ribosomal protein S18, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ58292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="217" mass="24620" checksum="1F963260475977CB" modified="2017-01-18" version="1">MFRNSRILGLISRPQFITRNFSTSRITNNSKPTLAETAAKFTQRESDISNIRGPGQSTTPKTVSSTKKIELIHKYVLYGSFTRNNTHLTLTAITEDINYLNRNPNLTYNDQVLYYVHLPQKVKISLSGGNVGFRKSARGEYEAGFQAAAKMFQLMEQKGYLDKDLEIIVKNFGKGREAFIDALKGKEGNRIRGKVVRLSDNTKLKFGGVRAPRRRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P070</accession>
    <name>A0A1E3P070_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_105540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037871.1">
        <property type="nucleotide sequence ID" value="XM_019180447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P070"/>
    <dbReference type="STRING" id="683960.A0A1E3P070"/>
    <dbReference type="GeneID" id="30197693"/>
    <dbReference type="OrthoDB" id="3981072at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030870">
        <property type="term" value="C:Mre11 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00027">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040227">
        <property type="entry name" value="Nibrin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12162:SF0">
        <property type="entry name" value="NIBRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12162">
        <property type="entry name" value="NIBRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="583"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="650"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="663"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="701"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="725" mass="82530" checksum="53AEA14987563803" modified="2017-01-18" version="1">MWLIKYTTEPKDDSEPQDILRWIQPNETFTFGRGERVTFSTHSSKVSKLHLQISILQSINNSSDLSQLKLLVDGKFTSIGQEVINKRLLPPEDLDKSIERILSSSTTIVLGKSGKRYDFIREPIIFNVTGCPDKYLRKLKDLDISYSSQFLKYTTHVVCSDMKVSTTTLMGMLKEIAIITPSFINSIDPKAIGQDFETNFPNIDDFLPDPNAKIRHGRSTMFKDILFVMVEGGQSTRFKNALEAGEGQVLILQLNKDTSKNELVKIIEDTLGGKQLVFISMIFQDEDFLKSVVDPSTKEYNSSKLLSELAKGFQKYEVTMEDIFNAVKDFNTDSILRTRVAKSEIQSSLPEDQPPLKKQKMNRKKKPQIKPLDSLDFFAGGGKFESSQTQPKTQVSETPEENLDEVEEPPKKKKQRNRVQSLANQMLDTNFKPSMNSIPESNDNSTGSLAPSNEQPQPLVQVEESIPKDEEDKENIQEPSKVEKQTSFHNAVINAKKSANQRINTQLGLDEIVTPDAIDQLQNLAIVEVTNIPMRQQKTSTTQSTNPQWAGRKNFKTFVKNGKSRIASKDQSFLYTRQFVPLKSFDPKYPNNPADELTKDFEKQQAQQDQVQSDDDENPVESFAFRNDLRQPTMVDKDQPATRSKLFVDQDSDSEVDDGLNMDVRRDEVSNVPEFRRRQEEEPVESVKQKKAIEKQIPIQVDEDDSDDGDDDDDDDDTPKFRFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P096</accession>
    <name>A0A1E3P096_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_18314</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_019180781.1"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:maintenance of RNA location"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="56"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="145" mass="16442" checksum="E6C50D186603D3AB" modified="2017-01-18" version="1" fragment="single">SGLLAKESNNIAGTQLKYTEPKDAASPPNSPDYYLFIFKKGSKIPREYKLNHKKFHLIGRDEKLVDLETDDDTCSKQHAVIQFRSRPITDDQGKTTQQVKPYIIDLESSNGTFLNGQEIPTSRFIELQAEDTIRFGISETDHVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0H3</accession>
    <name>A0A1E3P0H3_WICAA</name>
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        <recommendedName>
            <fullName evidence="9">MATE efflux family protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="7" type="ORF">WICANDRAFT_16331</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="30197931"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MATE_euk"/>
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        <property type="entry name" value="MATE_fam"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="178"/>
        </location>
    </feature>
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        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
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        <location>
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            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="326"/>
        </location>
    </feature>
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        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
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        <location>
            <begin position="406"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ58744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="49613" checksum="C3B0F954601CE037" modified="2017-01-18" version="1" fragment="single">VAVKDEVLNIARSALPLSITFFFEYLLAVNSLFLIGHLGSKELGSASLAVVTFNITGMAVFEGMATSLDTFCSQAFGARKYYKVGLYFQRCSVMIFTLSIPILMIWWWSKPILSYVVKEEELLDLTQIYLRILCFGAPGLIAFETGKRFLQAQRIFHASTYVLFICLPLNIVMNYFFIKLWGFIGAPIAIVVTYWLMAVLLLGYVVFVDGQQCWNGLSKRALRHWKPMLKLALPGLVMIESEYLSFEILTIMASYFGTDSLAAQSIISNIGSLWYQFPFAIGCAISTRVAIYVGSGSQRSSRVSVRIAFAVAAFTSLFSFLLLIFFKRPLTLMFTSDPHVVELSVKSMPILAINQLGDTFNIISAGILRSQGRQTIGSYLNVFAYYVVALPVSYLLAFHYKFEITGLWLGLTVGVTILGISETICVIKSNWNQIIKEALERNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0P3</accession>
    <name>A0A1E3P0P3_WICAA</name>
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        <recommendedName>
            <fullName evidence="4">ICE2-domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">WICANDRAFT_16276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_019180683.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0P3"/>
    <dbReference type="STRING" id="683960.A0A1E3P0P3"/>
    <dbReference type="GeneID" id="30197929"/>
    <dbReference type="OrthoDB" id="5577218at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048309">
        <property type="term" value="P:endoplasmic reticulum inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160031">
        <property type="term" value="P:endoplasmic reticulum membrane biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036228">
        <property type="term" value="P:protein localization to nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013635">
        <property type="entry name" value="Ice2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31726">
        <property type="entry name" value="PROTEIN ICE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31726:SF2">
        <property type="entry name" value="PROTEIN ICE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08426">
        <property type="entry name" value="ICE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="408" mass="46698" checksum="51BF4264BC821705" modified="2017-01-18" version="1" fragment="single">LRAALTTFYMVLIILTIPISFQIGGVFCGLAFTVTLFNIYWVLTTLSVLFKRNIILQVLYYFQHLLIPSLLTLFLSVFNNNNIDLLKNDNLIYYKFVLKPWRFFIINSTPLFTLLEGLCTILAIQSIGQTFEWLKYNKSESWVIMSLLSSGGIITTSLYFLYRIYSFPIEISILSASLIGAILTFCSGLGLYGIISKKGSTIESSLLFGYIVRCIYETFPELSNLASNEIGFLISSAANQLKNEIKHLPLIVNNDSLTHLFSFLTLNVPNSFQTIIEFLIATSKTITPSILINLAYRIAVFYSATRIIPALKNPVPSTKSMRLIYLFSPCIIIAVYTHLMMQYSNELDEELCIWGWWIDQAKNSGGDQVQIIVHPWQFWNWINMFSTLCVYAFEIFGEQSEEDHWKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0Q9</accession>
    <name>A0A1E3P0Q9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_79599</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038273.1">
        <property type="nucleotide sequence ID" value="XM_019184919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0Q9"/>
    <dbReference type="GeneID" id="30202165"/>
    <dbReference type="OrthoDB" id="4356994at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF12">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="8"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="798" mass="93200" checksum="8A391D756FEF5556" modified="2017-01-18" version="1">MPESRISSCVGCRLRRRKCDQKRPICTACDELKIPVELCVYRKMSLVSKDSKTTLSKLRVRNDELKIELAKCQARNGDGVDVDLERNPLFRTVRESNSSLTHFGSTSWAALIDTEPVLKAIVSQMHLLMKHQRDEYDSRKKSHHDKEMNMRPVMKGRTLLSQMGLEREDHLDFIKEMLQQVEDLLPSGEMIVHFLDMYKNYNAMKALGTFLVDFDSLNQAVLEFINVDLNGKATFNIQLPRDLDKMSHVLLFLSSLSFMIFLKNQVTVNNEGTTDVNYLVFLEFVDALFTLLHNYLAANGALRLLYTVETFQALFISGTFQNYVPHGNMIDDSEGAKTAVYMRKMVSLGKLLNLNKNIDVYYAHKSPQMRKSLKSLWYAVSSYDSLESLEIGIPPKIKVSFLRYDDFTSPFTETSITMNQVLKLYYEIELMKDTNQLIDIIENQIIKTLKDLLRNHFATLEEDVKVFQNYDFSHDSSSHDFFTRGQSFSTRCLIYSTIQTFYHICYHRLKIVHEIDSQRFKRITVLTWKYTLLVPFVMKELFKGYNRLVRHENSDAFFPVASLLMSVFPYMRLAQRRIAIFASARFMETFNYDQVSKTAKIFFYQSDNRENEIYEMLDDQSLYQKEYNLSDYSNLEIDDNPNELHSKFEELSDNKHIIVILAKTMKSSIQLLSNDRYNFNFVTYNYIFFFLLKMSNFFLNQAFPNTNFISRSNKSTPSSSTHTEFDFNELFEKAIDTRSQKFDYNNFFTNSYSSTFHPGDNNNQNHTRNGMLDDFFASLDIPGSSNVPTDSNTWFKHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P0R5</accession>
    <name>A0A1E3P0R5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">WICANDRAFT_63425</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038125.1">
        <property type="nucleotide sequence ID" value="XM_019183548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P0R5"/>
    <dbReference type="STRING" id="683960.A0A1E3P0R5"/>
    <dbReference type="GeneID" id="30200794"/>
    <dbReference type="OrthoDB" id="1747031at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990542">
        <property type="term" value="P:mitochondrial transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031921">
        <property type="term" value="P:pyridoxal phosphate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045315">
        <property type="entry name" value="Mtm1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760">
        <property type="entry name" value="FI19922P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760:SF2">
        <property type="entry name" value="FI19922P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="39"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="177"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="282"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ58918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="41939" checksum="DBA1F3D9DDF890A0" modified="2017-01-18" version="1">MSSEIAGVIEETEDLHLFPSKSPSTPPPTSSTQTKTRDITLTERMISACSGSIITSLIVTPLDVVRVRLQQQELLLPSTNCCKKQVFWEAPIAATSAAISSTLGNNKSPGCVTSDNVCITDKKMNGTWNAIYQISKSEGPTTLWRGLSLTLLMAAPANIVYFTGYEVLRDNSPIKHYDVLNPLLCGSIARVLAGTTVAPIELLKTRLQSIPSSAQNKTNALKNLLQNASQELKTKGPKTLFKGLELTLWRDVPFSGIYWASYEYFKNCFSNNLNVMANDEYNLFLHSFLSGSISGSIAALATNPFDVGKTRLQISIENNNGTLGTTSKNPSMFKFMSDIWKFEGFGALYVGIVPRVLKIAPSCAIMISSYELGKRFFSKTNVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P139</accession>
    <name>A0A1E3P139_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome assembly chaperone 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_79179</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037839.1">
        <property type="nucleotide sequence ID" value="XM_019184847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P139"/>
    <dbReference type="STRING" id="683960.A0A1E3P139"/>
    <dbReference type="GeneID" id="30202093"/>
    <dbReference type="OrthoDB" id="3980818at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12120">
        <property type="entry name" value="POC1 chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038605">
        <property type="entry name" value="Pba1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018855">
        <property type="entry name" value="Psome_chaperone_1_fun"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10450">
        <property type="entry name" value="POC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ58632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="27946" checksum="448259EF1C3454BE" modified="2017-01-18" version="1">MVFKPWTERAPRHIFEDISTLTSSPIPRIEISCSLTGQVLILPNTLHFIKEALLKQGDSKLIGGLKIFRIVKNQIESEEYIEDEQLYKANESNQEELSDEIEIYQINGINIIVTPNFQDVINYEIIARDIIKFPNIEILTITPGTLPFNCNIAEIGSSSTNFPQLSPPFIITGISASLVSNSKTQSKNNIQTLVLQSEGVSGFEKINNDSINELAHYLQDKFKLNSNFIKHVESLVNTGSSVNNFGLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P167</accession>
    <name>A0A1E3P167_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptide-methionine (R)-S-oxide reductase</fullName>
            <ecNumber evidence="5">1.8.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">WICANDRAFT_32976</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(R)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.12" evidence="5"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038141.1">
        <property type="nucleotide sequence ID" value="XM_019182019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P167"/>
    <dbReference type="STRING" id="683960.A0A1E3P167"/>
    <dbReference type="GeneID" id="30199265"/>
    <dbReference type="OrthoDB" id="44061at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028427">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00357">
        <property type="entry name" value="peptide-methionine (R)-S-oxide reductase MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46081">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46081:SF8">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="6">
        <location>
            <begin position="10"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ58934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="134" mass="15091" checksum="8B673094085BFEF7" modified="2017-01-18" version="1">MSKFPINKSNEEWRAILSPEQFQILRNKGTERPGTGEYWKTKANGIYNCAGCQNPIYKSSTKFDAHCGWPAFYEAIPGSLVYKTDETLGMTRTEMMCAKCGGHLGHVFKGEGYDTPTDERHCANSIALKFEPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P191</accession>
    <name>A0A1E3P191_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Agglutinin-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_79743</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024672">
        <property type="entry name" value="Agglutinin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033504">
        <property type="entry name" value="ALS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011252">
        <property type="entry name" value="Fibrogen-bd_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793:SF2">
        <property type="entry name" value="AGGLUTININ-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793">
        <property type="entry name" value="ALPHA-AGGLUTININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11766">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01056">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="Bacterial adhesins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133607" description="Agglutinin-like protein N-terminal domain-containing protein" evidence="13">
        <location>
            <begin position="20"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="Agglutinin-like protein N-terminal" evidence="14">
        <location>
            <begin position="50"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM01056"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ODQ59221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="638" mass="71277" checksum="ED10E562FBCECD06" modified="2017-01-18" version="1">MLRNYFLVFFASLIAIAYSKQIDEIFWSFNSIKLQEGTGYPYDLFRVNIGWEIDGGLNPQTGDLFSLDMPYVLDVRWVNETGFDTFEENFDIKTDDDVVIASCDFFQAAGRANSSKVNCRLTADVSIYRQIEGNITLDISFDAGGRNETIPAAKHWVSGQNTITFNEKLSQNITFQIPDPDTRGMVYQYDMNDELLMYYMLPSTLCPGKGIVGGSFYFYTYRGDLTTATSFVLWNMTDPELFVATELSPYGFPTAIDKLNILDTQTWNSGLKFKVQFGAIPAGQRMVYSFYNDPYYWNASSGAYMHFNPDVTVVCDDYSTATDFHYYRYRPNAPNSTDQYAVPTTVTTTNTWCSDTTSTTFQTGSGATVTEIINVAQKKTSWTTETTGGSFATTYTKNFTSDQTQVTVVVETSIPTTTYTTVTLPWNEGYTTLTTNSYSPGDMEIIVVEKTPAANFSSTTYITITKPWDEGFTSTYTNPYESGAKEIIVVVETPVSDYNATTITTVTKPWYEGYTRTYTQPFTSGETDIVVIVETPSTYNPTHTTTLTLPYDGPLNFPITFEYPYSSGDTAIIHIVQTPAPKSSSAMATSAPAVTGRARGVYDLSGDDVSSFLEPYKSMNVTLQYYNEELGKTIRTTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1H6</accession>
    <name>A0A1E3P1H6_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Knr4/Smi1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_105632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019037974.1">
        <property type="nucleotide sequence ID" value="XM_019180450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1H6"/>
    <dbReference type="STRING" id="683960.A0A1E3P1H6"/>
    <dbReference type="GeneID" id="30197696"/>
    <dbReference type="OrthoDB" id="2305498at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070880">
        <property type="term" value="P:fungal-type cell wall beta-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1580.10">
        <property type="entry name" value="SMI1/KNR4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018958">
        <property type="entry name" value="Knr4/Smi1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037883">
        <property type="entry name" value="Knr4/Smi1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051873">
        <property type="entry name" value="KNR4/SMI1_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47432">
        <property type="entry name" value="CELL WALL ASSEMBLY REGULATOR SMI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47432:SF1">
        <property type="entry name" value="CELL WALL ASSEMBLY REGULATOR SMI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09346">
        <property type="entry name" value="SMI1_KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00860">
        <property type="entry name" value="SMI1_KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160631">
        <property type="entry name" value="SMI1/KNR4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Knr4/Smi1-like" evidence="1">
        <location>
            <begin position="29"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ58767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="220" mass="25672" checksum="77DA5640E5D6D0A2" modified="2017-01-18" version="1">MVCDIGELVDSIKSIINKKFPSFDTFQDGLPHSEIRKFESEYQIKLPDEVVSYLQLVNGETPYDKENLFQLGVFLGLEMLSLDEIKREMNVWKEVVRDNPDFADDKYESTPEGAVKEVYCEPNFWVGLAIDGCGNSFGVDLSPEPNGNYGQVIIWGRDYIDERIVIFSNWAEFLKQVINDLNRDGEFYKIEDNSFEFLDEQVGNYMDYLFEKKLKEYRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1K3</accession>
    <name>A0A1E3P1K3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_22616</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038573.1">
        <property type="nucleotide sequence ID" value="XM_019180955.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1K3"/>
    <dbReference type="STRING" id="683960.A0A1E3P1K3"/>
    <dbReference type="GeneID" id="30198201"/>
    <dbReference type="OrthoDB" id="5592486at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11440">
        <property type="entry name" value="LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="3">
        <location>
            <begin position="124"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ59366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="316" mass="35364" checksum="24CAEBEAD689D55E" modified="2017-01-18" version="1" fragment="single">KDEYQAPKYPIVLCHGLSGFDRLILVPSLNLISNVVSGKKIEPEFHINDHGGVALDYWFGIQEALQAKGSTVIVAKVPGFGSIEERADELNRFISRATGYLRKQSKSEIYHEDAHPDQGKETDESFEQRQEKVKVNLIAHSMGGLDCRYLISKIENKNFEVVSLTTITTPHRGSEVADHVVDLAKNLPEDIKLPPSIYQLTTNYLKGFNKEVLNDPNVQYLSYGASFIPKWFNVFYGTWQIINEKAGPNDGMVSVDSAKWGIYLGTLVNVDHLDLINWTNGFKRLMGRISPGDLGPQIDTVALYLDIADNLAKRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1M8</accession>
    <name>A0A1E3P1M8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Vta1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">WICANDRAFT_63887</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VTA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038596.1">
        <property type="nucleotide sequence ID" value="XM_019183629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1M8"/>
    <dbReference type="STRING" id="683960.A0A1E3P1M8"/>
    <dbReference type="GeneID" id="30200875"/>
    <dbReference type="OrthoDB" id="391137at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.270">
        <property type="entry name" value="Vacuolar protein sorting-associated protein vta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044538">
        <property type="entry name" value="Vta1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039431">
        <property type="entry name" value="Vta1/CALS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023175">
        <property type="entry name" value="Vta1/CALS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041212">
        <property type="entry name" value="Vta1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46009">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VTA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46009:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VTA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04652">
        <property type="entry name" value="Vta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18097">
        <property type="entry name" value="Vta1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vta1/callose synthase N-terminal" evidence="10">
        <location>
            <begin position="13"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Vta1 C-terminal" evidence="11">
        <location>
            <begin position="311"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="164"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="175"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ59389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="39599" checksum="37D51F79E781E9DB" modified="2017-01-18" version="1">MPLTPPSSLPKAINPFVTRSNELENAEPLIAYFCKLYIVEQILNQGLHTTDDEISKFAMELLDDIENFKQVNQEDEIKDIINDKSSSILYIVGFGDKIFNNSLKQIKQHVSNKSTALSFLASVNFYELIKLWPDQDVYEMNELMKKIRYAKFHAARILKSLRKGEDPNEYDPPEFELKDEQEVPFKQEEEPTPAGLPNMEDDDDDDDNLGLPKVPQFIDDEPNKKQNQDQEPELSKEKSPSPSFKLPEPPKSKPGAIDVDPEPVTPRESTPKVNEPSSSRSLSQPIIKDNSTKPVTKFDVSQIIESGEIYNKAQKHAKFAISAMNYEDKETALKQLKEAIALLDQLEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1N3</accession>
    <name>A0A1E3P1N3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MAGE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">WICANDRAFT_63331</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ58822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038029.1">
        <property type="nucleotide sequence ID" value="XM_019183527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1N3"/>
    <dbReference type="STRING" id="683960.A0A1E3P1N3"/>
    <dbReference type="GeneID" id="30200773"/>
    <dbReference type="OrthoDB" id="205198at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1200">
        <property type="entry name" value="MAGE homology domain, winged helix WH1 motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1210">
        <property type="entry name" value="MAGE homology domain, winged helix WH2 motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037445">
        <property type="entry name" value="MAGE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041898">
        <property type="entry name" value="MAGE_WH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041899">
        <property type="entry name" value="MAGE_WH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002190">
        <property type="entry name" value="MHD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11736">
        <property type="entry name" value="MELANOMA-ASSOCIATED ANTIGEN MAGE ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11736:SF14">
        <property type="entry name" value="NSE3 HOMOLOG, SMC5-SMC6 COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01454">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01373">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MAGE" evidence="2">
        <location>
            <begin position="30"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ58822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="288" mass="32541" checksum="4478ABA46555DF44" modified="2017-01-18" version="1">MTESEEHSDYEAQPDQPQFGSSTSKVAVKVVRAALALNNSNIPITKANLQRHAWEDVPFGRANFQQALNLANSILNDTFGLTIEELPPKKSLNDGKKKESKTQQYILVNQLQDPYLKFQLESQWLSKSNALFQSELMEKRHDYRNNKELYPALSTDEGLVFSGLALAIVAIIMVSNNHINQVELIKIMKETFGVDPNTELEVLNITIEEFLKKLDKTDYVNRSCIKNDNEEVVEYSVGRRAKSEFNRESFIAFTQIIYNEPDGSPEFLAQINATIDATFGTVATGEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1T3</accession>
    <name>A0A1E3P1T3_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="10">PPIase</shortName>
            <ecNumber evidence="10">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">WICANDRAFT_63862</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 10">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the cyclophilin-type PPIase family. PPIL4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="10"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038572.1">
        <property type="nucleotide sequence ID" value="XM_019183625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1T3"/>
    <dbReference type="STRING" id="683960.A0A1E3P1T3"/>
    <dbReference type="GeneID" id="30200871"/>
    <dbReference type="OrthoDB" id="2083at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12235">
        <property type="entry name" value="RRM_PPIL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035542">
        <property type="entry name" value="CRIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45843">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45843:SF1">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="12">
        <location>
            <begin position="10"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="248"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="335"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="355"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="379"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="433"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="445"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ59365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="457" mass="53007" checksum="E72403024B97CA86" modified="2017-01-18" version="1">MSVILQTSKGNLVIDLFTKQAPTESKNFIKLCKAKYYNFAPFYNIQKDFIVELGDPLYPINKTGCSAWGLSDPSRGKSFKTSQNKLPKNRIGAVSLITQSDNTDPDERFATSIFTITLKDIEDRNVLKNNVVFGQVTEGFEILEAINNAYLDDDHSPLQDIRVHHTYILEDPFDDIEGLAIPPQSPEPTAEQLSTVKLADIEDIDLDEEEELTEERFKERQNREADSKALTLEIIGDLPSAEVKPMENVLFVCKLNPLTRDEDLEIIFSRFGKIISCEVMKDKSTGDSLQYAFIEYEEKKSCEAAYFKMDGVLIDDRRIHVDFSQSVSRLSEAWRNETNAKREKKHGGSTSDTSRNYRDRDYNRHGSSRGPYRSGQSSESRHGERYKSGEERSRDERYRSGRHGDGSRRDERYRDDRYKNDRERDHRHRSERHRSERERDSYHSSSHRHGGHHRDRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1T9</accession>
    <name>A0A1E3P1T9_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4a-hydroxytetrahydrobiopterin dehydratase</fullName>
            <ecNumber evidence="3">4.2.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">4-alpha-hydroxy-tetrahydropterin dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">WICANDRAFT_17706</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:11920"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="EC" id="4.2.1.96"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pterin-4-alpha-carbinolamine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.96" evidence="3"/>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038628.1">
        <property type="nucleotide sequence ID" value="XM_019180757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P1T9"/>
    <dbReference type="STRING" id="683960.A0A1E3P1T9"/>
    <dbReference type="GeneID" id="30198003"/>
    <dbReference type="OrthoDB" id="277398at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00488">
        <property type="entry name" value="PCD_DCoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="entry name" value="Transcriptional coactivator/pterin dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002017">
        <property type="entry name" value="PRK00823.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599:SF0">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
        <property type="entry name" value="PCD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ59421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="100" mass="11665" checksum="CC49B6CD021859EF" modified="2017-01-18" version="1" fragment="single">MINKITKIPVSKLSSEEIQKQLLNLSNWKYSNNEISKEYKLGNFEETWAFLNQISLRSHLWGHHPTITTTYNQVKITLTTHDVQGVSDIDIKFAKRIEGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P1X0</accession>
    <name>A0A1E3P1X0_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SWIRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">WICANDRAFT_33193</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454211">
        <property type="protein sequence ID" value="ODQ59240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="43"/>
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        </location>
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            <end position="331"/>
        </location>
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            <end position="335"/>
        </location>
    </feature>
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        <location>
            <begin position="517"/>
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        <location>
            <begin position="427"/>
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        </location>
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    <evidence type="ECO:0000256" key="1">
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    </evidence>
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        <source>
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        </source>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="62483" checksum="268BF6FD17560562" modified="2017-01-18" version="1">MNDAEETTATPQLPAETIIDYAEESRKIEEKAKAYLARQTKPVIVPSFAGWFDFEKIHEIEKKSLPEFFNSNSRFKTEKSYQDIRNFMIHTYRLNPTEYLTVTAARRNIASDVASIIRIHAFLEQWGLINYQIDPKTKPSLIGPQYTGHFQIVLDTPDGLKPFIPEDAKIINVEQDKQINGGGDEDESNNVPNGGDIPLNLELRRNIYDSTSDAVALNEQDRLSQSLNTKQFTCYVTGNDTTDVRYHNLRTKNSISARAFKEGHFGSNFHSSDFVRLEKLQKQGDAAPWNDQETLLLLEGVELFEDDWEKIANHIGSRTKEQSIAKFIQLPIEDKYLIKQLNDKKSVSKDSPKASSNTNDAVLKTIKFLIKNIDPELASKDLLQNDEDIQKAVKLTVGSIIGQSQLEQSSLQKDSSQLLENLVDLELSKVELKLNKLSILEKQLNAEKAELANQRKDILLDRLSLKKQSVSVRNKLLTAAKSSTPEEGAKLAQEALDIAVKAPRTVLIASAPSKQEATVVSSNEQEKNAVEQNENDQVVPVSLETPQNYQFWKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P203</accession>
    <name>A0A1E3P203_WICAA</name>
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        <recommendedName>
            <fullName evidence="4">Dolichol kinase</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">WICANDRAFT_13483</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
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            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ODQ59435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:CTP-dependent diacylglycerol kinase activity"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101026">
        <property type="term" value="P:mitotic nuclear membrane biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:regulation of isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="Dgk1-like"/>
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        <property type="entry name" value="CTP-DEPENDENT DIACYLGLYCEROL KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CTP-DEPENDENT DIACYLGLYCEROL KINASE 1"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
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        <location>
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            <end position="130"/>
        </location>
    </feature>
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        <location>
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            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="26866" checksum="622340D6C7AE5EF6" modified="2017-01-18" version="1" fragment="single">LTAHHIFGDYVRKHEIPRKVFHSSIGFITLYLYTKGLNKEIFPIPFHIAFIIIFTLDLIRLRWKYFNYLYCQVVGFLMREKEIQSYNGVLWYILGLDFVFSFFPKDICLASVLLLSWSDTAASTFGRAYGKYTPKIARNKSLAGSMAAFAVGVLSSYLLYGYFIPEYNYLNKVGEIYWTPESSYLNLHSMSLLAGFVAALSEGIDLYNWDDNFTIPTLSSIFFYIVITIFHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P2T9</accession>
    <name>A0A1E3P2T9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">WICANDRAFT_91128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038721.1">
        <property type="nucleotide sequence ID" value="XM_019186277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P2T9"/>
    <dbReference type="GeneID" id="30203523"/>
    <dbReference type="OrthoDB" id="10041630at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38421:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38421">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN USGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="290"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ59514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="394" mass="45497" checksum="D9E712FE140D68B0" modified="2017-01-18" version="1">MASPIFVSNPSPTFSWTAIFKGCQFAFLGAYRLLQNQLFFENPKYFKFTFVALQLSIVVQIVLSLPSIVTKFSYWILRFIVNKEIDIQKLNELISFFIYDVFQLQAIILLVIYTLYYDVFEEVFISGLQYIDQVTIANKKTSKNVYSTGLLKCKRVNLRNISLLSNWIAKLNRFSTHQINHLSQLLQSYLQLVMMNLFIYSCTHIPRLSSVVVSFIVSRSFNTKLGTSVTFLVFSMGLVTPPYVLLEFYSFYSITQLMVKYLLNIPYFQKLNFTDLQSDNWLYSRAGLSFGFGSVFTMLAWKFNYIALLILILQQLSLAYFISQVTDPVPESLTEPWILTQIYWTRIYNKLTVSGDGFKPIPLSYIISKHESSTPDTSAYVTPISSTTSLNRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3G9</accession>
    <name>A0A1E3P3G9_WICAA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">WICANDRAFT_62599</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039238.1">
        <property type="nucleotide sequence ID" value="XM_019183401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3G9"/>
    <dbReference type="GeneID" id="30200647"/>
    <dbReference type="OrthoDB" id="4080562at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ60031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="330" mass="37975" checksum="9114A28FFF96CF16" modified="2017-01-18" version="1">MKPSSTCLKHYSRSIFNPKNYRPHTIYESLPFTPKSLLCINTPTRIDTASSQLLVLTKKYPNLQIVSVTLDMKYVHAIGEVWFDELIGIESMYFSNKLWPDDTKFSAWIHNNKIDLNLSINLHNFKAKLFYNMIVKNPSSLKFSNYLSHLEISLPFDEKTTKFQIKSQLKPLTPRLKITSEIKDRCFIKSFNNKNAFLMFELLQKQNEEPGKEYKYVLEISSLSKTERFELFPIPSTRSLFILNRNVDFLPNDKLYARILQNCVNDVKFPKVNNGLVIDCFDMSNTDLRSSGEDVVVENSVIIGSGSGGFHVDDVAFASFGDYVKVESVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3K5</accession>
    <name>A0A1E3P3K5_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="13">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">WICANDRAFT_83935</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 13">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12 13">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 13">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 13">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="13"/>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039130.1">
        <property type="nucleotide sequence ID" value="XM_019185624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3K5"/>
    <dbReference type="STRING" id="683960.A0A1E3P3K5"/>
    <dbReference type="GeneID" id="30202870"/>
    <dbReference type="OrthoDB" id="10265628at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000097">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF57">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 13" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9 12 13" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="14">
        <location>
            <begin position="37"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ59923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="490" mass="54182" checksum="EA6492B0890AFC8D" modified="2017-01-18" version="1">MLRLQLRSIARTPLAGRLARNYAVSTKSQELISKNVQEIDPEMADILKSERLRQKSSITLIPSENFTSKAVMDLLGSEMQNKYSEGYPGERYYGGNEFIDKAESLCQKRALEAFHLDPSLWGVNVQPLSGAPANLYAYSAVLNVGDRLMGLDLPHGGHLSHGYQTASTKISFISKYFQTMPYRLDESTGLIDYDALEKSAELFRPKIIVAGASAYSRIIDYERIRKIADKVGAYVLSDMAHISGLVSAGVTPSPFPYSDIVTTTTHKSLRGPRGAMIFFRKGLRKTTKKGKEIFYDLEKKINFSVFPAHQGGPHNHTISALAVALKQAQSPEYKTYQENVVSNAKHFADVLQSKGFNLVSNGTDTHLILIDLRSKKIDGARLEAVLERINIAANKNTIPGDKSALFPSGLRVGTPAMTTRGFEQKEFDQVADYIDRAVKVALELKNNAKGDDARALLADFKRLADESDDVKALGKEVAEWVSQYPVPGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P3Y8</accession>
    <name>A0A1E3P3Y8_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome biogenesis protein SLX9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">WICANDRAFT_78252</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in ribosome biogenesis. Required for normal pre-rRNA processing in internal transcribed spacer 1 (ITS1). May be involved in the movements of the replication forks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with the 35S, 23S and 20S pre-rRNAs and with the U3 snoRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLX9 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019038819.1">
        <property type="nucleotide sequence ID" value="XM_019184690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P3Y8"/>
    <dbReference type="STRING" id="683960.A0A1E3P3Y8"/>
    <dbReference type="GeneID" id="30201936"/>
    <dbReference type="OrthoDB" id="4068648at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051880">
        <property type="term" value="F:G-quadruplex DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028160">
        <property type="entry name" value="Slx9-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15341">
        <property type="entry name" value="SLX9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="34"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ59612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="189" mass="21777" checksum="548FF9EF95BFE472" modified="2017-01-18" version="1">MAAKKRVNLRAKVASKASNKKPIQPSSIDNQLSEDPKAFLHEYKESKREKQDTRSKEFLNRLSSKDDNLSGISKSSIRRRKRKQKAELLPKMQDLLTSLEDTTSDNKEEDKSDKFANINIVDRKTGNKYIEHVQKRKNLPNPKNQKGAKVIEKMEREKFVQVLQDPNFRANPFATLRESIAARLKNGTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P419</accession>
    <name>A0A1E3P419_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">WICANDRAFT_78570</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ59944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039151.1">
        <property type="nucleotide sequence ID" value="XM_019184750.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P419"/>
    <dbReference type="STRING" id="683960.A0A1E3P419"/>
    <dbReference type="GeneID" id="30201996"/>
    <dbReference type="OrthoDB" id="413079at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051788">
        <property type="entry name" value="MFS_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23514">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23514:SF3">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="350"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="447"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="72"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ59944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094112"/>
        </source>
    </evidence>
    <sequence length="472" mass="52720" checksum="F81F664BD4657D91" modified="2017-01-18" version="1">MASIKKNAKVVYPGLEASSLITERTLSYGSIDELSPLELERLLATTQEITYKSQTIHIHSKNWRHSTYLKCQVLACFLNMFLMGMDGQTIGSLIPTLSEYYQQHDFNISFVFILQFLGYTSSALFMDKIHRRIGRRGVLSLGSFLVCLTSGINATKPPFIVFVGAYLCSGVGGGLMDGCMNVFISSLVDHNELMGILHGSFGIGGIVVPIMISYLLKWFDGDFKVHYLTMCGLNLFSCVFSLMSFKNETSVKYEYEVSLEANDECHEETSLSVILKNKTVLIFSLFLFLYTGSETSISSWLVSYLIKVKHLNQLSSSYAISWFWIGITVGRVTLGFMTSRLFLNEYRANVFYTAMALMVYSSYTTFTLMITSYQISAFSELTDTLMFVEGLFMGPLFPTSTVALMKLLPVNLRVGSMGVVATLSGSGSALFPFFIGTITHLTKLDYLPIYICFLTAGYLVIWLSIPKSCSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3P486</accession>
    <name>A0A1E3P486_WICAA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleolar protein Dnt1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">WICANDRAFT_78907</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Hansenula anomala</name>
        <dbReference type="NCBI Taxonomy" id="683960"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Phaffomycetales</taxon>
            <taxon>Wickerhamomycetaceae</taxon>
            <taxon>Wickerhamomyces</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454210">
        <property type="protein sequence ID" value="ODQ60301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019039508.1">
        <property type="nucleotide sequence ID" value="XM_019184805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3P486"/>
    <dbReference type="STRING" id="683960.A0A1E3P486"/>
    <dbReference type="GeneID" id="30202051"/>
    <dbReference type="OrthoDB" id="6365676at2759"/>
    <dbReference type="Proteomes" id="UP000094112">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018844">
        <property type="entry name" value="Dnt1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043185">
        <property type="entry name" value="Net1/Tof2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28196">
        <property type="entry name" value
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